LOCUS       DAVLVF010000001        67353 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVLVF010000001 DAVLVF010000000
VERSION     DAVLVF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 67353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67353
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.001"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..448)
                     /locus_tag="VYN66_000001"
     CDS             complement(<1..448)
                     /locus_tag="VYN66_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1494098.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNS"
     gene            570..1316
                     /locus_tag="VYN66_000002"
     CDS             570..1316
                     /locus_tag="VYN66_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1494099.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            1329..2309
                     /locus_tag="VYN66_000003"
     CDS             1329..2309
                     /locus_tag="VYN66_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV1494100.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            2504..2824
                     /locus_tag="VYN66_000004"
     CDS             2504..2824
                     /locus_tag="VYN66_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1494101.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            2870..3178
                     /locus_tag="VYN66_000005"
     CDS             2870..3178
                     /locus_tag="VYN66_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1494102.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3195..4493
                     /locus_tag="VYN66_000006"
     CDS             3195..4493
                     /locus_tag="VYN66_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1494103.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSWYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            4644..7082
                     /locus_tag="VYN66_000007"
     CDS             4644..7082
                     /locus_tag="VYN66_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV1494104.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIGGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKAALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            7101..7769
                     /locus_tag="VYN66_000008"
     CDS             7101..7769
                     /locus_tag="VYN66_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV1494105.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLVLAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            7787..8875
                     /locus_tag="VYN66_000009"
     CDS             7787..8875
                     /locus_tag="VYN66_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV1494106.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            9330..11831
                     /locus_tag="VYN66_000010"
     CDS             9330..11831
                     /locus_tag="VYN66_000010"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV1494107.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            12025..12720
                     /locus_tag="VYN66_000011"
     CDS             12025..12720
                     /locus_tag="VYN66_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1494108.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            12732..13148
                     /locus_tag="VYN66_000012"
     CDS             12732..13148
                     /locus_tag="VYN66_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1494109.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(13775..13867)
                     /locus_tag="VYN66_000013"
     CDS             complement(13775..13867)
                     /locus_tag="VYN66_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV1494110.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            14028..14999
                     /gene="ruvB"
                     /locus_tag="VYN66_000014"
     CDS             14028..14999
                     /gene="ruvB"
                     /locus_tag="VYN66_000014"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV1494111.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHAGLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            14980..15552
                     /locus_tag="VYN66_000015"
     CDS             14980..15552
                     /locus_tag="VYN66_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV1494112.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            15783..16541
                     /locus_tag="VYN66_000016"
     CDS             15783..16541
                     /locus_tag="VYN66_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV1494113.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            16550..17353
                     /locus_tag="VYN66_000017"
     CDS             16550..17353
                     /locus_tag="VYN66_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV1494114.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKIRGLATMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            17375..18634
                     /gene="rseP"
                     /locus_tag="VYN66_000018"
     CDS             17375..18634
                     /gene="rseP"
                     /locus_tag="VYN66_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV1494115.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRHKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            18647..20500
                     /locus_tag="VYN66_000019"
     CDS             18647..20500
                     /locus_tag="VYN66_000019"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV1494116.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPTLLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            20600..21979
                     /gene="bglA"
                     /locus_tag="VYN66_000020"
     CDS             20600..21979
                     /gene="bglA"
                     /locus_tag="VYN66_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1494117.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            22171..23979
                     /gene="glmS"
                     /locus_tag="VYN66_000021"
     CDS             22171..23979
                     /gene="glmS"
                     /locus_tag="VYN66_000021"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV1494118.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVIDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            24098..25147
                     /locus_tag="VYN66_000022"
     CDS             24098..25147
                     /locus_tag="VYN66_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV1494119.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(25247..29044)
                     /gene="spuA"
                     /locus_tag="VYN66_000023"
     CDS             complement(25247..29044)
                     /gene="spuA"
                     /locus_tag="VYN66_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV1494120.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWHLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDTHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVKIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQEASV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            29460..29873
                     /gene="rpsL"
                     /locus_tag="VYN66_000024"
     CDS             29460..29873
                     /gene="rpsL"
                     /locus_tag="VYN66_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV1494121.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            29893..30363
                     /gene="rpsG"
                     /locus_tag="VYN66_000025"
     CDS             29893..30363
                     /gene="rpsG"
                     /locus_tag="VYN66_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV1494122.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            30787..32868
                     /gene="fusA"
                     /locus_tag="VYN66_000026"
     CDS             30787..32868
                     /gene="fusA"
                     /locus_tag="VYN66_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV1494123.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            32972..37363
                     /locus_tag="VYN66_000027"
     CDS             32972..37363
                     /locus_tag="VYN66_000027"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV1494124.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGIEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNAAFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            37465..37728
                     /locus_tag="VYN66_000028"
     CDS             37465..37728
                     /locus_tag="VYN66_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV1494125.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            37721..37999
                     /locus_tag="VYN66_000029"
     CDS             37721..37999
                     /locus_tag="VYN66_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV1494126.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            38020..38172
                     /locus_tag="VYN66_000030"
     CDS             38020..38172
                     /locus_tag="VYN66_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494127.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            38194..39435
                     /locus_tag="VYN66_000031"
     CDS             38194..39435
                     /locus_tag="VYN66_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV1494128.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            39445..39675
                     /locus_tag="VYN66_000032"
     CDS             39445..39675
                     /locus_tag="VYN66_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1494129.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            40033..40755
                     /locus_tag="VYN66_000033"
     CDS             40033..40755
                     /locus_tag="VYN66_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1494130.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            40972..42306
                     /gene="pepC"
                     /locus_tag="VYN66_000034"
     CDS             40972..42306
                     /gene="pepC"
                     /locus_tag="VYN66_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV1494131.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(42362..43273)
                     /locus_tag="VYN66_000035"
     CDS             complement(42362..43273)
                     /locus_tag="VYN66_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1494132.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(43297..44100)
                     /locus_tag="VYN66_000036"
     CDS             complement(43297..44100)
                     /locus_tag="VYN66_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV1494133.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(44128..45117)
                     /gene="manX"
                     /locus_tag="VYN66_000037"
     CDS             complement(44128..45117)
                     /gene="manX"
                     /locus_tag="VYN66_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV1494134.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(45350..46369)
                     /gene="adhP"
                     /locus_tag="VYN66_000038"
     CDS             complement(45350..46369)
                     /gene="adhP"
                     /locus_tag="VYN66_000038"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV1494135.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWAVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(46721..47533)
                     /locus_tag="VYN66_000039"
     CDS             complement(46721..47533)
                     /locus_tag="VYN66_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1494136.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            47723..49141
                     /locus_tag="VYN66_000040"
     CDS             47723..49141
                     /locus_tag="VYN66_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV1494137.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLVITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            49194..49901
                     /locus_tag="VYN66_000041"
     CDS             49194..49901
                     /locus_tag="VYN66_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1494138.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            49996..50970
                     /gene="folP"
                     /locus_tag="VYN66_000042"
     CDS             49996..50970
                     /gene="folP"
                     /locus_tag="VYN66_000042"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV1494139.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALATGADLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            50972..52294
                     /locus_tag="VYN66_000043"
     CDS             50972..52294
                     /locus_tag="VYN66_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1494140.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALGDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            52275..52829
                     /gene="folE"
                     /locus_tag="VYN66_000044"
     CDS             52275..52829
                     /gene="folE"
                     /locus_tag="VYN66_000044"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV1494141.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            52872..53684
                     /gene="folK"
                     /locus_tag="VYN66_000045"
     CDS             52872..53684
                     /gene="folK"
                     /locus_tag="VYN66_000045"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV1494142.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(53729..54046)
                     /locus_tag="VYN66_000046"
     CDS             complement(53729..54046)
                     /locus_tag="VYN66_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV1494143.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            54403..54849
                     /gene="rplM"
                     /locus_tag="VYN66_000047"
     CDS             54403..54849
                     /gene="rplM"
                     /locus_tag="VYN66_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV1494144.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            54868..55260
                     /gene="rpsI"
                     /locus_tag="VYN66_000048"
     CDS             54868..55260
                     /gene="rpsI"
                     /locus_tag="VYN66_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV1494145.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(55522..55944)
                     /locus_tag="VYN66_000049"
     CDS             complement(55522..55944)
                     /locus_tag="VYN66_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1494146.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            56091..56267
                     /locus_tag="VYN66_000050"
     CDS             56091..56267
                     /locus_tag="VYN66_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494147.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            56669..58882
                     /locus_tag="VYN66_000051"
     CDS             56669..58882
                     /locus_tag="VYN66_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV1494148.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(59104..59580)
                     /locus_tag="VYN66_000052"
     CDS             complement(59104..59580)
                     /locus_tag="VYN66_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV1494149.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQAQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFSKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            59803..63006
                     /locus_tag="VYN66_000053"
     CDS             59803..63006
                     /locus_tag="VYN66_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV1494150.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKGG
                     AITISSHEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYYWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVGRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            63386..63771
                     /gene="tnpA"
                     /locus_tag="VYN66_000054"
                     /pseudo
     CDS             63386..63771
                     /gene="tnpA"
                     /locus_tag="VYN66_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(64150..64779)
                     /locus_tag="VYN66_000055"
     CDS             complement(64150..64779)
                     /locus_tag="VYN66_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV1494151.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(64789..65790)
                     /locus_tag="VYN66_000056"
     CDS             complement(64789..65790)
                     /locus_tag="VYN66_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV1494152.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(65821..66462)
                     /locus_tag="VYN66_000057"
     CDS             complement(65821..66462)
                     /locus_tag="VYN66_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV1494153.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(66481..67296)
                     /locus_tag="VYN66_000058"
     CDS             complement(66481..67296)
                     /locus_tag="VYN66_000058"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV1494154.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT        20008 a        12257 c        15147 g        19941 t
ORIGIN      
        1 tagaattata atgttttagg tctgtgtaga taaaatccac atattgaatt aaatcaataa
       61 atttttcatg atcaacaaag gcagtagttt caatggcagt gtgtatgtga tgttctttag
      121 ctgattttaa gatggcttta gcaaattcaa actgagcaaa tatttcacct cctgataaag
      181 ttaaacctcc accggattct tcgtaaaatt ctttgtcttt taatacctct gtaataattt
      241 cttctacagt cttttcttca ccgactaagg tgaatttctc tcgttgagca tctttcattt
      301 tttcaggttt cattctttga gattcaggat tagaacacca tggacagcgc agaggacatc
      361 cttttaaaaa aacagttgta cgaatacccg ggccgtcatg aattgaaaag tgttgaatat
      421 taaaaataat tcctttagat atttccataa gagttcctcc tcttcataag tctatcctat
      481 cacaaaaacg aaagaaaaac aattacgaac gaaagtttaa tttgttgttt tttgcttttt
      541 attttggtag aataaaacgg aggtgtaaaa tgaaaagatt ggaacaaatt attaaattag
      601 tatcagaata tgaaaagatc gacgttaata cattatcgga aaaattaaat gtatcgaaag
      661 taacgattag aaaagattta gataaattag agtcaaaagg tttattacac agagagcatg
      721 gatatgctgt attaaatagt ggagatgact taaatgtacg tttgtcaatt aactatgaaa
      781 ttaagagaaa aattgttcag gaagcagtaa aattggtgtc agataatgaa acaataatga
      841 tagaatctgg atcgacctgt gctttacttg ctgaggaaat ttgcaagcaa aaaagaaatg
      901 ttacgattgt aacaaattca ttttttatag caaattttgt gagagcttat gattcatgtc
      961 gtattattgt tcttggtgga gagtttcaga aggattcaca ggttactgtg ggacctttat
     1021 taaaagaaat gatacagact tttcatgtgc atcaagcttt tgttgggaca gatggttacg
     1081 ataaagagat gggctttaca ggaaaagatt taatgcgcag tgaggtagtt caatatattt
     1141 cagcagcgtc ggataaagtc attgtactaa ctgactcaag taaatttgat aaaaggggta
     1201 cagtaagaag atttgcttta agtcaagtct atgaagtaat aacagacgaa aaactttcta
     1261 aacaaaatat agctacatta gaaaatgctg ggataatggt taaggtagtt tcgtaagagg
     1321 ttaaatgtat gaatcaagat aggaataaac tgctctctaa aattgcttat ctgtattata
     1381 ttgaaaactt aaatcagtca caaatagcag caaaattagg aatttataga acctctatta
     1441 gtagaatgtt aacagaagca aggaatgcag gaattgttaa aattgaaata gagaattttg
     1501 ataccaatat gtttaagttg gaaaattatg taaaagaaaa atatggtctg gaaagtttag
     1561 aaattattcc aaatgaattt gatgataatc caacaatttt atctgaaaga atttctcaag
     1621 ttgcagcagg tgtccttagg aatctaattg atgataatat gaaaattggc ttttcttggg
     1681 ggaaaagttt aagtaattta gtagatttaa ttcacagtaa aagtgtccga aatgttcact
     1741 tctatcctct agcaggtggt cctagtcaca tacacgctaa ataccatgtg aatacactga
     1801 tttatgaaat gtctagaaaa tttcatggag agtgtacatt tatgaatgca acgattgtgc
     1861 aagaaaataa attgttagca gatggtattt tgcaatcaag atattttgaa aatttgaaaa
     1921 atagttggaa agatttagat atagctgtag tcggaattgg tgattttagc aataaaggaa
     1981 agcatcaatg gttagacatg cttacagagg atgactttaa agaattgacc aaagttaaaa
     2041 ctgtaggaga aatttgttgt cgattttttg attcaaaagg taaagaagtc tatgaaaact
     2101 tacaggaaag aacgatagca atctctttgg aagatttaaa aaatattcct caaagtttag
     2161 ctgttgctta cggtgatacg aaagtatctt cgattctttc tgtcttgcgt gctaatttag
     2221 taaatcattt gattacagac aaaaatacaa ttttaaaagt tttggaagaa gatggggatt
     2281 tgacttttag agagattcta ggtgagtgaa aatgatagac tgatcagttt atcgtttttt
     2341 ctttttagtc gattgcacat ttgtgctcat ataaacaaaa atagtttatc tgttgttttt
     2401 ggattgacaa ctttattatg tagttgtatt ctatagttac aaaagaaaat tttaaaattt
     2461 caaatgaaaa aagcttttta catagtgaaa tgaggaggaa tttatggaaa ttattgttcc
     2521 agatcaaatt atcatgggtt taattttata tgctggtgat gcgaaacaac atatttataa
     2581 agcgttagat tacataaaaa atggtacatg tgaacggtgt gaagaagaaa tccagttagc
     2641 tgatgccgcc ttattagaag cccataatct acaaacaaaa ttcttggcac aagaagcgtc
     2701 tggtacaaag acagaaatta cagctctctt tgttcattca caagatcatc tcatgactag
     2761 tatgacggag attaatttaa tcaaagaaat tattagtttg agaaaagaac ttcataaaaa
     2821 ataatactag agtattatca ttgttattaa catagaggag gaaaacataa tggtgaagat
     2881 tggtttgttt tgtgcagcag gtttttctac tggtatgctt gtaaataata tgaaaattgc
     2941 agcgcaagct agtggagttg aggcagaaat agaggcgttt tctcagtcta aattagcgga
     3001 ttatgcgcca aatatagatg ttgcactatt gggtccacaa gttgcttata cattagataa
     3061 atcaaaagaa atttgtgata agtgtgatgt tccgatagct gttattccga tgatggacta
     3121 tggtatgtta gatggaaaaa aagtattaga tttggcccta tctttgatta gtgggtaaga
     3181 aaaggagatt tattatggca aagatggatg ttcagaaaat cattgcaccg atgatgaagt
     3241 ttgtgaatat gcgtggcatt atagctctaa aagatgggat gttagcaatt ttgccattga
     3301 cagtagttgg tagtttattc ttgattatgg gacaattgcc gttcgaagga ttaaataaga
     3361 gcattgctag tgtttttgga gctaattgga cagagccgtt tatgcaagta tattcaggaa
     3421 cttttgctat tatgggtcta atttcttgtt tttcaattgc ctattcttat gctaagaata
     3481 gcggagtaga ggctttacca gctggagttc tatctgtatc tgcattcttt attttgctaa
     3541 gatcatctta tatccctaaa caaggtgagg caattgggga tgctattagt aaagtttggt
     3601 ttggaggtca aggaattatc ggtgctatca ttataggttt ggtagtagga agtatttata
     3661 ccttctttat aaagagaaaa attgttatta agatgccaga acaagttcca caagctattg
     3721 ccaaacagtt tgaagcaatg attccagcat ttgtaatttt cttactttct atgattgtat
     3781 atattttagc gaagtcattg actaatggcg gaacattcat agaaatgatt tattctgcta
     3841 ttcaagttcc attgcaaggt ttaactggat cttggtatgg tgcaattgga attgcattct
     3901 ttatatcatt tttgtggtgg tttggtgttc atggacaatc ggtagtaaat ggagtagtga
     3961 cagctctgct tttatctaat cttgatgcta ataaagctat gttagcctct ggtaatctat
     4021 cattagaaaa tggtgcacat attgttactc aacaattttt agattcattt ttaattctat
     4081 caggttcagg gattacgttt ggtcttgtag ttgccatgct ttttgcagca aaatcaaaac
     4141 aataccaagc cttaggaaaa gttgccgctt ttccagcaat atttaacgta aatgagccag
     4201 ttgtatttgg atttccgatt gtcatgaatc cagttatgtt tgtacctttc attcttgttc
     4261 ctgtacttgc agctgtgata gtatatggag ctattgcaac aggtttcatg cagccattct
     4321 caggggtaac attgccttgg agtacaccag ctattttatc aggatttttg gtgggtggat
     4381 ggcaaggagt tattactcag ctggtgatat tagcgatgtc tacattggtt tattttccat
     4441 tctttaaagt acaggatcgt ttagcttacc aaaatgaaat caaacaatct tagaggtatt
     4501 tgtgtgttac tgttaaactc acacatttgt gctaaaaatt agagagttaa aatttttcta
     4561 gttaaaagct tgaaaatttc tataaaaatc ggtattatat tttcgaaaga aataaaaata
     4621 ttttcgaaag aaaggtggtt acgatggtaa atacagaagt agcaagaaca acaatcaaga
     4681 cagaatattt tggcagcctt actgaaagga tgaacaaata tcgagaagat gttttaaata
     4741 aaaaacctta tattgatgct gagagagcag ttctagcaac acgcgcctat gaacgataca
     4801 aggaacaacc taatgtccta aaacgtgcat atatgctgaa agaaattttg gaaaatatga
     4861 ctatctatat tgaagaagaa tctatgattg ggggaaatca agcttcttcc aataaagatg
     4921 ctcctatttt tccggaatat acgctagaat ttgttctcaa tgagttggat ctttttgaaa
     4981 agcgtgatgg agatgttttc tatattacag aagaaacaaa agaacaactt agaagtattg
     5041 ctccgttttg ggaaaataat aatttacgtg ctagagctgg tgccttatta cctgaagaag
     5101 tgtctgttta tatggaaaca ggattcttcg gtatggaagg taagatgaat tctggagatg
     5161 ctcacctagc agttaactat cagaaacttt tgcaatttgg cttaagaggt tttgaagagc
     5221 gggctcgtaa agcaaaagca gctctagatt taacagatcc agcaagtatt gataaatatc
     5281 atttttacga ctctatattt atcgtaatcg atgctattaa agtatatgca aagcgctttg
     5341 ttgctcttgc taaaagttta gccgaaaatg caaatcctaa acgtaagaaa gaattacttg
     5401 agattgcaga tatttgctct agagtcccat atgaaccggc aactactttt gcagaagcta
     5461 ttcaatcagt ttggtttatt caatgtattt tacaaattga atctaatggc cactctcttt
     5521 catatggccg ttttgatcaa tatatgtatc catatatgaa ggctgattta gaaagtggta
     5581 aagaaacaga agatagcatt gttgaacgtc tgacaaatct ttggattaag acaattacaa
     5641 ttaataaggt tcgcagtcaa tcacatacat tttcttcagc aggaagtcct ttatatcaaa
     5701 atgttacaat tggtggacag actcgagata agaaggatgc tgttaaccca ttatcttatt
     5761 tggtattaaa atcagttgca caaacccatc taccgcaacc taatctaact gtacgttacc
     5821 atgcaggttt agatgctcgt ttcatgaatg agtgtattga agtgatgaaa cttggttttg
     5881 gtatgcctgc atttaataat gatgagatta ttattccttc ttttattgca aaaggagtat
     5941 tggaagatga tgcttatgat tacagtgcca ttggatgtgt tgaaacggca gttccaggga
     6001 aatggggcta tcgttgcaca ggtatgagtt atatgaactt ccctaaggtt ctacttatca
     6061 cgatgaatga tggaattgat ccggcttcgg gtaaacggtt tgcaccaagc tttggtcatt
     6121 ttaaggatat gaagaacttt tctgaattag aaaatgcttg ggataaaaca ctaagatatt
     6181 tgacacgaat gagtgttatt gttgaaaatt ctattgattt atcattggaa cgagaagttc
     6241 ctgatattct atgttcagca ttgactgatg attgtattgg tcgtggaaaa caccttaaag
     6301 aaggtggagc agtatatgat tatatatcag gattgcaagt tggaattgca aatttgtcgg
     6361 attcattagc tgcaattaaa aaattggtgt ttgaggaaga acgtataagc ccaagtcagc
     6421 tttggcatgc attggaaaca gattatgccg gagaagaagg taaggtcatt caagaaatgt
     6481 tgattcatga tgcacctaag tatggtaatg atgatgatta tgctgacaaa ttggttactg
     6541 ctgcttatga catttatgtt gatgaaattg ctaaatatcc taatacacgt tatggaagag
     6601 ggcctattgg aggaattcgt tattcaggaa catcttctat atcagccaac gtagggcagg
     6661 gacgtggaac attagcaact ccagatggac gcaacgcggg tacaccgtta gcagagggtt
     6721 gttcaccatc acataatatg gatcaacacg gccctacatc tgttttaaaa tctgtttcaa
     6781 aattaccaac agatgaaatc gtaggtgggg ttctcttaaa tcagaaagta aatcctcaaa
     6841 cgttagccaa agaagaagat aaattaaaac taattgcttt gttacgaaca ttctttaatc
     6901 gtttacatgg gtaccatatt caatacaatg ttgtttccag agagacgttg attgacgctc
     6961 agaaacatcc tgaaaaacac agagacttaa ttgttcgtgt tgcaggatac tctgcattct
     7021 tcaatgttct ttctaaggca acccaagatg acattatagg acgtactgag catactttgt
     7081 aaaataaaga ggttcttttt atggaattta tgcttgacac attaaattta gatgagatta
     7141 aaaagtggtc tgaaattttg ccgctagctg gggtaacttc aaatcccact attgcaaaaa
     7201 gagagggttc tattaatttt tttgaacgaa tcaaagatgt aagagaattg attggctcca
     7261 caccctctat tcatgttcag gtgatttctc aagattttga aggcatctta aaggatgctc
     7321 atgaaattcg aagacaagca ggagatgata tatttatcaa agtacctgtt actccagctg
     7381 gattacgtgc aataaaggtg ctaaaaaaag agggctacca tatcactgca acagctattt
     7441 atacagttat tcagggatta ttagctatcg aagcaggagc ggattaccta gctccatatt
     7501 ataatagaat ggaaaatctg aacattgatt caaattctgt cattcgtcaa ttagttcttg
     7561 ctattgatag acagaactct cctagtaaga ttttggctgc atcctttaaa aatgtagcac
     7621 aagtaaataa tgctttagct gcaggtgcgc atgctgttac agcaggagca gatgtttttg
     7681 aatcagcttt cgccatgcca tctatccaaa aggcggttga tgatttttct gacgattggt
     7741 ttgttactca aaatagtcgt tccatttaga tagagaggaa atacatatga gaatttttgc
     7801 tagtccttct agatatattc agggggaaaa tgccttgttt gaaaatgcca aatcaatttt
     7861 ggatttggga aattacccta ttctattatg cgatcagttg gtttatgata ttgttggaaa
     7921 acgatttgaa gattatctac ataggtatgg tttccatatt gttctggcgc tatttaatgg
     7981 tgaagcttct gacaatgaaa tcaatcgagt tgttgccttg gctgagaaag aaaattgtga
     8041 tagtattatc ggtcttggtg ggggaaagac gattgatagc gcaaaagcta ttgcagattt
     8101 gattgaaaag cctgttatta ttgctccaac aattgcatcg accgacgcac ctgtatctgc
     8161 tttatctgtt atttatacag atgaaggtgc atttgatcat tatctatttt attctaaaaa
     8221 tccagattta gttttggttg atacaaaagt tatttcacaa gcccctaagc gtttattagc
     8281 gtctggtatt gcagatggtt tagcaacttg ggttgaggcg cgtgcggtta tgcaggcaaa
     8341 tggaaaaact atgttgggac aacagcaaac attggctgga gttgcaattg cgaagaaatg
     8401 tgaagaaacg ctgtttgcag atggtttaca ggctatggca gcttgtgaag ctaaagtggt
     8461 gacaccagca ttagaaaata ttgttgaagc taatacttta ttgagtggtc taggttttga
     8521 aagtggagga ttagctgcgg cgcatgcaat tcataatggt tttactgcat tgacaggtga
     8581 cattcatcat ttaacacatg gtgaaaaagt agcttatgga actttagtac aactattatt
     8641 ggaaaataga cctaaagaag aacttgataa gtatattgag ttttacaaaa aaattggtat
     8701 gccaacaact ctaaaagaaa tgcatttgga tcaagttgga tatgatgatt taataaaagt
     8761 tggtaaacaa gcaactatgg agggtgagac aattcatcag atgccgttta agatttcgcc
     8821 ttcagatgtt gctcaagcta ttatcgctgt agatgcctat gtaaattcaa aataaacaat
     8881 aaggactact gttttccaaa tggtagtctt ttattgatcc ccgtattgaa ttctatagaa
     8941 gattgaaata ggatgagaac aaatcgattg ggaaagtaaa attaatttct ataaatgttt
     9001 tagcaattgt ttcgtactat ttcagattca gtctactata cgttcttcat aaatcaaaaa
     9061 gcgacatagg ttgtcggcta tttattgtga atacattaat tagcattcca gttttatctt
     9121 cggtctaaaa taagtatttt gtgctatacg agataagcgt cttgacttac tccttgattt
     9181 actgcataac aatgggataa aaagtgggag atagagcaat tcatagtcat caaaattaat
     9241 gagatacagt atacagtttt tcctttaaac acatttcaaa ttccctcaaa aatggtataa
     9301 tagtaacatc acaaaattgg agagagacca tgagttttta caatcataaa gaaattgagc
     9361 ctaagtggca gggctactgg gcagaacatc atacatttaa gacaggaaca gatgcatcaa
     9421 aacctaagtt ttatgcgctt gatatgttcc cttatccgtc tggagctggt ctgcacgtag
     9481 gacacccaga aggttatact gcaaccgata tcctcagtcg ttacaaacgt gcgcaaggct
     9541 acaatgtcct tcacccaatg ggttgggatg cttttggttt gcctgcagag caatacgcta
     9601 tggatactgg taatgaccca gcagaattta cagcggaaaa cattgccaac ttcaaacgtc
     9661 aaatcaatgc gcttggattt tcttatgact gggatcgtga agtcaataca acagatccaa
     9721 actactacaa gtggactcaa tggattttca ccaagcttta cgaaaaaggc ttggcctatg
     9781 aagctgaagt gccagtaaac tgggttgagg aattgggaac tgccattgcc aatgaagaag
     9841 tgcttcctga cggaacttct gagcgtggag gctatccagt tgtccgcaaa ccaatgcgcc
     9901 aatggatgct caaaatcacg gcttacgcag agcgcttgct caatgactta gatgaactag
     9961 attggtcaga gtctatcaag gatatgcaac gcaactggat tggtaaatca actggtgcca
    10021 atgtaacttt caaagtaaaa ggaacagaca aggaatttac agtctttact actcgtccgg
    10081 acacactttt cggtgcgact ttcactgtct tggctcctga acatgaatta gtagacgcta
    10141 tcacaagttc agagcaagca gaagctgtag cagactataa acaccaagcc agccttaagt
    10201 ctgacttggt tcgtacagac cttgctaagg aaaaaactgg tgtttggaca ggtgcctatg
    10261 ccatcaaccc tgtcaatggt aaggaaatgc caatctggat tgcggactat gttcttgcta
    10321 gttatggaac aggtgcggtt atggctgtgc ctgcccacga ccaacgtgac tgggaatttg
    10381 ccaaacaatt tgaccttcca atcgtcgaag tacttgaagg tggaaatgtc gaagaagctg
    10441 cctacacaga ggatggcctg catgtcaatt cagacttcct agatggattg aacaaagaag
    10501 acgctattgc caagattgtg gcttgcttgg aagaaaaagg ctgtggtcag gagaaggtta
    10561 cctaccgcct ccgcgactgg ctctttagcc gtcaacgtta ctggggtgag ccaattccaa
    10621 tcattcattg ggaagatgga acttcaacag ctgttcctga aactgaattg ccgcttgtct
    10681 tgcctgtaac caaggatatc cgtccttcag gtactggtga aagtccacta gctaacttga
    10741 cagattggct tgaagtgact cgtgaagatg gtgtcaaagg tcgtcgtgaa actaacacca
    10801 tgccacaatg ggctggttca agctggtact acctccgcta tattgacccg cacaatactg
    10861 agaaattggc tgatgaggac ctcctcaaac aatggttgcc agtagatatc tacgtgggtg
    10921 gtgcggaaca tgctgtactt cacttgcttt atgctcgttt ctggcataaa ttcctctatg
    10981 acctcggtgt tgttccgact aaggaaccat tccaaaaact ctttaaccaa gggatgattt
    11041 tgggaacaag ctaccgtgac caccgtggtg ctcttgtggc aaccgacaag gttgaaaaac
    11101 gtgatggttc cttctttcat atagaaacag gggaagagtt ggagcaagcg ccagccaaga
    11161 tgtctaaatc gctcaagaac gttgttaacc cagatgatgt ggtggaacaa tacggtgccg
    11221 atacccttcg tgtttatgaa atgtttatgg gaccactcga tgcttcgatt gcttggtcag
    11281 aagaaggttt ggaaggaagc cgtaagttcc ttgaccgagt ttaccgtttg attacaagta
    11341 aagaaatcct tgcggaaaac aatggtgctc ttgacaaggc ttacaacgaa acagtcaaag
    11401 ctgttactga gcaaattgag tctctcaaat tcaacacagc tattgcccaa cttatggtct
    11461 ttgtcaatgc tgctaacaag gaagataagc tttatgttga ctatgccaaa ggctttattc
    11521 aattgattgc accatttgca cctcacttgg cagaagaact ctggcaaaca gtcgcagaaa
    11581 caggtgagtc aatctcttat gtagcttggc caacttggga cgaaagtaaa ctggttgaag
    11641 acgaaatcga aatcgttgtc caaatcaaag gtaaagtccg tgccaaactc atggtcgcta
    11701 aagacctatc acgtgaagaa ttgcaagaaa ttgctctagc ggatgaaaaa gtcaaagcag
    11761 aaattgacgg taaggaaatc gtgaaagtga ttagtgtacc aaataaattg gttaatattg
    11821 ttgtaaaata accaatttat ttgctgattt ccaacctcaa acagtttccc aaactgtttg
    11881 agccaactaa actcgttaat atcgttgtga aataaggtag gaatccttca gagtagaatc
    11941 tggaggattt tttgaatctt cttatgaaag tatggtatac tatgggcaac tataaagttt
    12001 gaaaagtgaa aaaaggagaa agctatgcca gtaaatgaat atggtcaaat gattggggag
    12061 tcaatggaag gttatacacc aggtgaactg ccttctattg atttcttaga agggcgttat
    12121 gctaggatag aggctctttc agtggaaaag catgcggagg atttgctagc tgtttatggc
    12181 ccggatactc ctcgggagat gtggacctac ctttttcagg agccagtagc agatatgggg
    12241 gagctggtta gccttttaca tcagatgttg gctcgtaagg accgttttta ctatgcaatc
    12301 atagacaagg cgactggtaa ggctttggga tctttttctc tcatgcgcat tgaccagaat
    12361 aaccgagtaa tagaagtcgg agcagtcact ttttctccag agctaagggg tacacggata
    12421 ggaactgaag ctcagtatct cctagctcgc tatgtttttg aggagctaaa ctatcgtcgc
    12481 tacgagtgga aatgcgatgc cttaaatctg ccatccagac gagctgcgga gcgtttgagc
    12541 tttatttatg aaggaacctt ccgtcaggca gtggtttata agggacgtac gagagatacg
    12601 gattggttgt ctatgattga taaggactgg cctcaagtca aagatcgttt ggaaacatgg
    12661 ttgcgtcctg aaaattttga taaaaatgga cagcagtaca agagcttgag agaactttaa
    12721 gaggtgttga gatgattact attaaaaagc aagaaattgt caagctagag gatgttttac
    12781 atctctatca ggctgtcggt tggacaaatt atacccatca accagagatg ctggagcagg
    12841 ccttatctca ttcattagta atttatctgg cacttgatgg tgatgctgtg gtgggcttga
    12901 ttcgtttggt tggagatgga ttctcatcag tattggttca ggatttaatc gttttaccaa
    12961 tctatcagcg tcaaggaatt ggtagtgcct tgatgaaaga ggctttagag gattacaaag
    13021 atgcctatca agtccagctg gtgacagaag agacagaaag aaccttgggc ttctatcgtt
    13081 ctatgggctt tgaaatctta tccacctata attgtatagg gatgacttgg atgaatcgta
    13141 aaaaataaca aaacttgttt tttcttaagc aaaatttaag gatggtctag tattatatag
    13201 tcattaaata aagacctcct aactttattt aataaaatcc taaacttttc tttttcataa
    13261 taatctccct taacttcacc caatcaggtg gagtttttta gctctatttc aggcttttgg
    13321 ggactattct aaaaataatt tttcgatatt tttcggtatt tttcggattt tggtcgggga
    13381 attggcgggg acttttttag cgaatataac taagaaatag gtctgtggtc gcttcagcta
    13441 gttcaaactc aatttgattg taagtgactg ttaattgaag aactggaact actgtaataa
    13501 tgattttgct tgtctagtag tggcgtatta aatgagaagt aacatgtaag attgtctctt
    13561 cgtttacgaa atcaaaattt ctatgaaatt gatagaatcc ctaacgttct cttaatctta
    13621 accgtcttgt cgtacagcgg ttgaagaaaa tgtaaacctt tgacaaaatt cgtgaactat
    13681 gggataataa aaaggaattg agtactagtt ctatatcata ggctagaaaa gaccccaagc
    13741 tcacgaccaa ataagcttga ggtctttttt gacactattt gtccttgttt agccatttat
    13801 cgactatacg aagaacgaca ccgaccacaa ttggtccgat gatagttttg agtactaatt
    13861 ccatcatggg ctatctcacc tcctttcgta ggcggtgtag cagtgtcgta gaatattata
    13921 ccacataagt gctaaactta ggagtcaccc aatcaggtgg cttttttgtt tgtggcttgg
    13981 ttttttgata taatagagcc atgagtagaa ttttagataa tgagataatg ggggatgagg
    14041 agttagtaga acgcacgctc cgtcctcagt atttacgtga atatatcgga caggataagg
    14101 tcaaggacca gctacaaatc tttattgaag ctgccaaaat gcgggatgaa gcgctggatc
    14161 atgtgctctt atttgggcct ccaggtctcg ggaaaacgac catggccttt gttattgcca
    14221 acgaactggg agtcaatctt aagcagacgt cgggtccagt cattgaaaaa gccggagatc
    14281 tggtagctat tttgaatgag ttagagcctg gggatgtact ttttattgat gagatccatc
    14341 gtttgccaat gtcagtggaa gaggtgcttt atagtgctat ggaggacttc tacatcgata
    14401 ttatgattgg ggctggtgag ggtagtcgta gtgttcattt ggagttacca ccttttacct
    14461 tgattggtgc gacgactcgg gctggtatgc tctccaatcc gctacgggca cgttttggga
    14521 ttacaggcca tatggagtat tatgcccatg ctggcttgac agaaattgtc gagcggacgg
    14581 cagatatttt tgagatggaa atcactcatg aggcagcatc tgagttggcc ctacgtagtc
    14641 gtgggacccc tcgtattgcc aatcgtctcc tcaagcgcgt gcgcgatttt gcccagataa
    14701 tggggaatgg ggtaattgat gatattatta ccgataaggc tttgactatg ctggatgttg
    14761 accatgaagg tttggactat gtggatcaaa aaatccttcg taccatgatt gagatgtaca
    14821 gtggaggacc tgttggtcta ggaactcttt ctgtgaatat cgccgaagag cgtgagacag
    14881 ttgaagatat gtatgagcct tacttgattc aaaaaggttt tatcatgcgg acacggtctg
    14941 gacgggtggc gactgctaag gcatatgagc acttaggtta tgaatacagt gaaaaataag
    15001 caagaaatct tagaggcttt tagagaaaat ccagatatga tggccattct gacgatcatc
    15061 cgagaccttg gtctgaaaga ctcgtggttg gcagcaggtt ctgtcagaaa tttcatttgg
    15121 aatcttttgt cagacaaatc cccttttgat catgaaacag atatagatgt gattttcttt
    15181 gatccagatt tttcttatga ggaaacctta ttactggaga aaaagctgcg agaggatttt
    15241 cctcagtacc agtgggaatt gaaaaatcag gtctatatgc atcagcacag tcctcacact
    15301 gcttcctata ccagttctcg tgatgctatg agtaagtatc cagaacggtg tacgacagtt
    15361 ggactgcgct tgaatgaaga atcagatttt gaactctatg caccttatgg tttggaggat
    15421 attttgaatt ttcaagttcg tccaactcct catttcttag aaaatgaaga ccgaatggaa
    15481 ctctatcaaa cacgtttatc caagaaaaat tggcaggaga aatggaaaaa tttgattttt
    15541 aaaaatactt aaggaaactt taagctaggg agtgtacact aagttcataa gttaagaaga
    15601 ccttaactta aactcctaaa actttttcat aataatctcc ctataaaaat aaagtcgcct
    15661 aatcaggcgg cttatttttt tgaaaaatgg gcttggtgcc tgagaataaa tagcttagtg
    15721 atagaagaaa atggggaaat atggtataat gaaacgatag atttttgaat aggaataaga
    15781 tcatgtttgg attttttaag aaagataagg ctgtggaagt agaggttccg acacaggttc
    15841 ctgctcatat cggcatcatc atggatggca atggccgttg ggctaaaaaa cgtatgcaac
    15901 cgcgagtttt tggacacaag gcgggcatgg aagcattgca aaccgtgacc aaggcagcca
    15961 acaaactggg cgtcaaggtt attacggtct atgctttttc tacggaaaac tggacccgtc
    16021 cagatcagga agtcaagttt atcatgaact tgccagtaga gttttatgat aattatgtcc
    16081 cggaactaca tgcgaataat gttaagattc aaatgattgg ggagacagac cgcctgccta
    16141 agcaaacctt tgaagcttta accaaggctg aggaattgac taagaacaac acaggattga
    16201 ttcttaattt tgctcttaac tatggtggac gtgctgagat tacacaggcg cttaagttga
    16261 tttcccagga tgttttagat gccaaaatca acccaggtga catcacagag gaattgattg
    16321 gtaactatct ctttactcag catttgccta aggacttacg agacccagac ttgattatcc
    16381 gtactagtgg agaattacgt ttgagcaatt tccttccatg gcagggagcc tatagtgagc
    16441 tttattttac ggacacctta tggcctgatt ttgacgaagc agccttgcag gaagctattc
    16501 ttgcctataa tcgtcgccat cgccgatttg gaggagttta ggaggaaata tgacccagga
    16561 tttacagaaa agaaccttgt ttgcagggat tgccctggct attttcctac caattttaat
    16621 gattgggggc ctcttgcttc agatagcaat cggaatcata gccatgctag ccatgcatga
    16681 acttttgaag ataagaggtc tagcgaccat gacgatggag ggcctcttga ctctctttgc
    16741 aacctttgca ttaaccattc ccttggagaa ttacctgact tttttgccag ttgatgggaa
    16801 tgtggttgcc tatagtgttt tgatttcaat catgttagga acgaccgttt ttagcaagtc
    16861 ttatacgatt gaggatgcgg ttttccctct tgctatgagc ttctacgtgg gctttggatt
    16921 taatgcttta ctagatgctc gtgttgcagg tttggacaag gctctcttag ccttgtgtat
    16981 cgtctgggcg acagacagtg gtgcctatct tgttgggatg aactatggga aacgaaagtt
    17041 agcaccaagg gtatcgccta ataaaaccct tgagggtgcc ttgggtggta ttttaggagc
    17101 aattttagta accattatct ttatgatagt tgacagtaca gttgctcttc catatggaat
    17161 ttacaagatg tcagtctttg ctattttctt tagcattgct ggacaatttg gtgatttact
    17221 agaaagttcg atcaaacgtc attttggtgt taaggattct gggaaattta tccctggaca
    17281 tggtggtgtt ttggatcgtt tcgatagtat gttgcttgta tttccaatca tgcacttatt
    17341 tggactcttt taatcaaaag acggaggaaa cgctatgctc ggaattttaa cctttattct
    17401 ggttttcggg attattgtag tggtgcacga gttcgggcac ttctactttg ccaagaaatc
    17461 agggatttta gtacgtgaat ttgccatcgg tatgggacct aaaatttttg ctcacattgg
    17521 caaggatgga acggcctata ccattcgaat cttgcctctg ggtggctatg tccgcatggc
    17581 cggttggggt gatgatacaa ctgaaatcaa gacaggaacg cctgttagtt tgacacttgc
    17641 tgatgatggt aaggttaaac gcatcaatct ctcaggtaaa aaattggatc aaacagccct
    17701 ccctatgcag gtgacccagt ttgattttga agacaagctc tttatcaaag gattggttct
    17761 ggaagaagaa aaaacatttg cagtggatca cgatgcaacg gttgtggaag cagatggtac
    17821 tgaggttcgg attgcacctt tagatgttca atatcaaaat gcgactatct ggggcaaact
    17881 gattaccaat tttgcaggtc ctatgaacaa ttttatctta ggtgtcgttg ttttttgggt
    17941 tttaatcttt atgcagggtg gtgtcagaga tgttgatacc aatcagttcc atatcatgcc
    18001 ccaaggtgcc ttggccaagg taggagtacc agaaacggca caaattacca agattggctc
    18061 acatgaggtt agcaactggg aaagcttgat ccaagctgtg gaaacagaaa ccaaagataa
    18121 gacggcaccg actttggatg tgactatttc tgaaaagggg agtgacaaac aagtcactgt
    18181 tacacccgaa gatagtcaag gtcgttacct tctaggtgtt caaccggggg ttaagtcaga
    18241 ttttctatcc atgtttgtag gtggttttac aactgctgct gactcagctc tccgaattct
    18301 ctcagctctg aaaaatctga ttttccaacc ggatttgaac aagttgggtg gacctgttgc
    18361 tatctttaag gcaagtagtg atgctgctaa aaatggaatt gagaatatct tgtacttctt
    18421 ggcaatgatt tccatcaata ttgggatttt taatcttatt ccgattccag ccttggatgg
    18481 tggtaagatt gtgctcaata tcctagaagc catccgccac aaaccattga aacaagaaat
    18541 tgaaacctat gtcaccttgg ccggagtggt catcatggtt gtcttgatga ttgctgtgac
    18601 ttggaatgac attatgcgac tcttttttag ataatcgagg aatattatga aacaaagtaa
    18661 aatgcctatc ccaacgcttc gcgaaatgcc aagcgatgct caagttatca gccatgctct
    18721 tatgttgcgt gctggttatg ttcgccaagt ttcagcaggt gtttattctt atctaccact
    18781 tgccaaccgt gtgattgaaa aagctaaaaa catcatgcgc caagaattcg aaaagattgg
    18841 tgctgttgag atgttggctc caacccttct tagtgcagaa ttgtggcgtg aatcaggtcg
    18901 ttacgaaacc tatggtgaag acctttacaa actaaaaaac cgtgaaaaat cagactttat
    18961 cttaggtcca actcacgaag aaacctttac agctattgtc cgtgattctg ttaaatctta
    19021 caagcaattg ccactcaacc tttatcaaat tcagcccaag tatcgtgatg aaaaacgccc
    19081 acgtaatgga cttcttcgta cacgtgagtt tatcatgaag gatgcttata gtttccacgc
    19141 taactatgat agtttggata gtgtttatga tgagtacaaa gcagcctatg agcgtatttt
    19201 cactcgtagt ggtttagact tcaaggctat tattggtgac ggtggagcca tgggtggtaa
    19261 ggatagccaa gaatttatgg ccattacatc tgctcgtaca gaccttgacc gctgggttgt
    19321 cttggacaag tcagttgcct catttgacga aattcctgca gaagtgcaag aagaaatcaa
    19381 ggcagaattg ctcaaatgga tagtctctgg tgaagatacc attgcttact caagtgagtc
    19441 tagctatgca gctaacttag aaatggcaac aaacgagtac aaaccaagca accgtgttgt
    19501 cgctgaagaa gaagttactc gtgttgcaac gccagatgtt aaatcaattg atgaagttgc
    19561 agccttcctc aatgttccag aagaacaaac gattaaaacc ctcttctaca ttgcagatgg
    19621 tgagcttgtt gcagcccttc tagttggaaa tgaccaactc aacgaagtca agttgaaaaa
    19681 tcacttggga gcagatttct ttgacgttgc tagcgaagaa gaagtggcga atgttgttca
    19741 agcaggattt ggttcacttg gaccagttgg tttgccagag aatattaaaa ttattgcaga
    19801 tcgtaaggtg caagatgttc gcaatgcagt tgtcggtgct aacgaagatg gctaccactt
    19861 gactggtgtg aacccaggcc gtgattttac tgcagaatat gtggatatcc gtgaagttcg
    19921 tgagggtgaa atttccccag atggacaagg tgtccttaac tttgcgcgtg gtattgagat
    19981 cggtcatatt ttcaaactcg gaactcgcta ctcagcaagc atgggagcag atgtcttgga
    20041 tgaaaatggt cgtgctgtgc caatcatcat gggatgttac ggtatcggtg tcagccgtct
    20101 tctttcagca gtgatggagc aacacgctcg cctctttgtt aacaaaacgc caaaaggtga
    20161 ataccgttac gcttggggaa tcaatttccc taaagaattg gcaccatttg atgtgcattt
    20221 gattactgtc aatgtcaagg atgaagaagc gcaagccttg acagaaaaac ttgaagcaag
    20281 cttgatggga gctggttatg aagtcttgac agatgaccgt aacgaacgtg tcggtgttaa
    20341 atttagcgat agtgacttga ttggtcttcc aatccgtatc actgttggta aaaaagcggc
    20401 cgatggcatt gtagaagtta agatcaaggc aactggtgac accattgagg ttcatgcaga
    20461 taacgtgctt gaaacgcttg aaatcctaag caagaaataa aaactataat cagaagaaaa
    20521 acaaggaaaa aatgtaacta gtttttacct tgttttttct gtataatggg agaaatgagt
    20581 agataaagag gtaaatgtta tgctaagatt tccaaaggat tttgtctggg gatcctctac
    20641 ttctggaccg cagacagaag gacgtgtagc tggtgacggt aagggagaca atctctggga
    20701 ttactggttc caagtggagc caaatcgtta ctataatggg attggttcag ataagacatc
    20761 gactttttat gaaaattggg agcgggatat tgagcttttg ttagagactg gtcacacagt
    20821 ctttcggact tctattcagt ggtcacggat ttttccacaa ggctgtggaa aagtcaaccc
    20881 tcaaggtgtg gatttttatc gtaaggtctt tgaggctatt aaggctaaag gaattcgtct
    20941 gttagtcaat ctctatcatt ttgatttacc ttttgctctt caagaggatg gtgatggttg
    21001 ggaaaataag gcgacagtct cagcctatga agactatgct cgtttttgtt ttgagactta
    21061 tggagattta gtggatcaat ggattacctt taacgagccc atcgttcctg tagaatttgg
    21121 ttatttttat gatgcccatt atccacataa ggtggatgca gaggcagctg ttaaggtagc
    21181 ctatcataca caattggcca gcagccgggc tgttaaggct tgccatgaac ttttgcctga
    21241 ttccaagatt gggattgtcc tcaacttgac accggcttat ccacgtagcc agcatcctgc
    21301 tgatgtcaag gcagctcgta ttgcggccct ttttcaggcc caatctttct tagatccatc
    21361 tgtcttgggg acttatccac aggagttggt agaaatcttg catgaacacg gtcttttacc
    21421 tgatgctaca gaggaagagt tggaactcat tcgtgataat acggtggact tccttggtgt
    21481 gaactactat caacctttgc gtgttatggc tcctcgattt gctaagcatc cagatagtcc
    21541 actcttacca gaacattttt acgagcctta tgtgatgcct ggacgtaaaa tcaattctca
    21601 ccgtggttgg gagatttatg agcaagggat ttatgacatc gcccaaaata tcaaggaaaa
    21661 ttatggcaat attgagtgga tgttgactga gaatggtatg ggtgttgaag gggaagaaaa
    21721 attccgtcaa gatggaatga ttcaagatga ttaccgtatt gactttgtaa aaggtcatct
    21781 tcgtgaactt caccgtgcca ttgaagatgg tgccaactgt aagggctact tgatttggac
    21841 ctttattgac tgctggtcat ggctcaacag ctataaaaat cgctatggtt tggtcgaatt
    21901 agacttggaa acgcaagaac gtcgtctgaa gaaatcaggc cactggttca aagaattaag
    21961 cgataataat ggattttaaa aaggactctg actgattatc ccaatcggtc agagtttttt
    22021 gcttacagga gcttaatttg aactatacca atttttactc ttgacaagtg aaagaaacaa
    22081 gtatatactg tttttgctga ttctataaaa gatgaattag actataccaa ttttaaggag
    22141 aaagcacagc ttgcctgtgt cgtatatact atgtgtggaa ttgttggtgt tgttggaaac
    22201 acaaatgcaa ctgatatttt gattcaaggg cttgaaaagc ttgaataccg tggctatgat
    22261 tctgcgggaa tttttgtcct agatggtgct gataaccatt tggtgaaggc tgttggtcgt
    22321 atcgcagaat tgtctgccaa gacagctggt gttgagggaa caactggtat cggacatact
    22381 cgttgggcaa ctcatgggaa accaacggaa gacaatgctc acccacaccg ctctgagaca
    22441 gaacgttttg tcttggttca caatggggtg attgaaaact accttgaaat taaagaagaa
    22501 taccttgctg ggcaccactt caaagggcaa acagatacgg aaatcgccgt acatttgatt
    22561 ggaaaatttg cggaagaaga cggtctctca gttcttgaag cctttaaaaa agctcttcat
    22621 attatccgtg gttcatatgc ctttgccttg attgactctg aaaatccaga tgtcatctat
    22681 gtagcgaaaa acaaatctcc acttttgatt ggtcttgggg aaggctacaa tatggtctgc
    22741 tcagatgcta tggctatgat tcgtgaaacc aaccaataca tggaaattca tgaccaagag
    22801 ttggtaatcg tcaaggctga tagcgtggaa gttcaagact atgatggtaa cagtcgtgaa
    22861 cgtgctagct atactgcgga acttgacttg tcagatatcg gtaagggaac ttatccttac
    22921 tacatgctta aggaaattga tgagcaacca actgttatgc gtaaactcat tcaagcctac
    22981 acggatgatg ctggtcaagt agtgattgat cctgctatca ttaaggctgt tcaagacgca
    23041 gaccgcatct acatccttgc agctggaaca tcttaccatg caggatttgc ttctaagaaa
    23101 atgttggaag aattgacaga tacaccagtt gaacttggca tctcatctga gtggggctac
    23161 ggtatgccac ttctcagcaa gaaaccactc ttcatcttta tcagccaatc tggtgaaaca
    23221 gcggatagtc gtcaagtttt ggtcaaggct aatgaaatgg gaattccaag cttaacagtg
    23281 acaaacgttc caggttcaac cctctcacgt gaagccaact ataccatgct ccttcacgca
    23341 ggtcctgaaa ttgccgtggc atcaactaaa gcctatacag cgcaaatcgc agcccttgcc
    23401 ttccttgcaa aagcagtcgg agaagcaaat ggtaatgcta aagcgcaagc ctttgacctg
    23461 gttcatgaat tgtcaatcgt agctcagtct atcgaatcaa ctctttcaga gaaagaaacc
    23521 attgaagcca aggttcgtga acttcttgaa acaactcgta acgcctttta catcggacgt
    23581 ggtcaagatt actacgtagc catggaagca agtctcaaac tcaaagagat ttcttatatc
    23641 cagtgtgaag gttttgcggc aggagaactc aagcacggaa ccattgcctt gattgaagaa
    23701 ggaacacctg tcttggctct cttgtcagat ccagtccttg ccaaccacac tcgtggaaat
    23761 atccaagagg tggcagcccg tggtgctaag gtcctcacta tcgcagaaga aaatgttgct
    23821 aaagataccg acgatatcgt ccttacgact gtacaccctt acctctcacc aatttcaatg
    23881 gtcgtaccga cgcaattggt tgcttacttt gcaaccctcc accgtggcct cgatgtggat
    23941 aaaccacgta accttgctaa atcagtaacg gtagaataag ctaaaaaagt ctagttatct
    24001 aggctttttc taatgagcaa attggttgct tgtactcaag attcgtgtta gaataatttt
    24061 tcaaattgaa ggacagagat agacaaggag aatcacaatg gtagaattgg gaatttcaac
    24121 atttggggaa ataacggagc ttgaagggac tggacaaact tacagtcatg ctgaacgcat
    24181 tcgccagttg gtggcagaga ttgagctggc tgacaaggtt ggtttggacg tatatgggat
    24241 tggcgagcac catcgggcgg attttgcagt atcagcccca gagattgtcc tggccgctgg
    24301 ggcagtcaat accaagaaaa tccgtttgac cagtgcagtc agcattctgt caagcatgga
    24361 tccgattcgt ttgttccaac agtatgccac tatcgatgct ttgtcaaatg gacgatcgga
    24421 gattatggct ggaaggggct ctttcacgga atctttccct ctgtttggtt atgacttgaa
    24481 agactacgac tctctttttg atgagaaatt agacttgctt cagttagtca atgaaaagac
    24541 caagttagac tggcaaggtc gattgactca aacgattgct ggtaaagaag tttatcctcg
    24601 cccagttcag gacaaattgc ccttgtggat agctacaggt ggtcatgtcg aatcaacagt
    24661 gaagattgct caggctggtc taccgattgt atatgctatt attggtggta atccacgtta
    24721 ttttaaaaag ttgattcagg cttatcgtga gattggaagc gaagcgggcc atgctgacaa
    24781 agacttgaaa gtgggagccc attcttgggg gtggattgcg gaagatggtg agcaggcggt
    24841 gaaagattat ttccatccga ctaagcaagt ggtggatgct atttccaaag accgtccgca
    24901 ctggcaggaa ttgcgttatg agcaatattt ggagcaggta gggccaaatg gcgccatgtt
    24961 tgttggcaat ccagatcagg tggccgaaaa attgattcgc atgatagagg atttaggatt
    25021 ggaccgcttc atgcttcatc taccgcttgg ttctatgcct catgaccagg ttctgagagc
    25081 tattgaactc ttcggaacac aagttgcacc caaagtacga gcttattttg ctatgaaaga
    25141 ggcttaataa aaaatcctaa tcaagtttat taggacaaca aatattgtac aaaaattaat
    25201 cccccgagct ataatgcttg ggggattttt ctataggagg gctagtttag ttctcttttt
    25261 tattttttag taagaaaccg agaccaagga gcgcaaggag gctgattcct gcaaatagga
    25321 gtttgttttc gtttttgatt cctgtatttg gaagttcagc ttgtttatct ggggttgcag
    25381 gtatattttc cttgctagag ttttctaccg attcgtttcg aaccgcttct tttacagatg
    25441 cttcttgtgc ttcttgtgct ggttgttcag cttgtgaatc agctgtagct tggctaggtt
    25501 tattttccgc atcagctact ttggcatctg gtttagtaga atcaggtcta gcttctggag
    25561 ctgggtcttg atgtgcaggg tgagaagctt cattttgatg ttcaggcttg gaaggggttg
    25621 agtctggttt ctcttctgca gttgactcat ggctagttcc attttgagag actcgaagaa
    25681 cagtagctgt aagggcattc aatttcaagc ctttttcagt ccattcaagt cctttcgggt
    25741 tggcaatttt gactgatcct gcttggtttt catctgccaa aacttccgca tttcttagat
    25801 gtgcaaaggc agttcccaaa ttaaattcgc gagctttttc atccgcattg acaaagactg
    25861 cgtagatatc gccgtttgga gcagtgattt ggtagccaat cactacatcc tctttttcca
    25921 caccattttg gcctgggaca gtgatgaggt ggacacggtc tttgatatct tggagactct
    25981 taagtcggaa ggcatctgta gattgacgaa gggcaatcaa acctttcata tagtcacggc
    26041 tcttgacatt ttcaggataa gctttaccat ctgtagcctt agtccagtca aacttgttga
    26101 ctgcgtcgct agaatcgtaa gaatcatgga tgaagtaagg atagtcaaat gggttgccgt
    26161 ccttatcacg caacaagtga gatttgtttg gaaccttatc ctctgctact ggagtcttgt
    26221 aggctgggtc acggaattgt ttagtacgtc catattcctg accggagtgg ataaatggag
    26281 ttccttgagc tgtcaagacc atgagatttc caagtcgtaa acgacggtgg atttcagcat
    26341 agttctcagc cttgcttggg tcttttttga tagactgggc aatgatgtca aagagggtca
    26401 agttatcatg ggctgctatg tattggatga catctccagg gctgtcagct tcaaagttag
    26461 ttggttgagc aatgagattt ttaaagatgg tgttgacatc acgcttgcca cctgtgataa
    26521 aggcaggttg accttcgttt ggataaccag atttgaggtt gttacggatg tcatctgaaa
    26581 agacagcgac agtatcggta tgtttcatcc aatcttggtc agcagcttta gtaggcatgt
    26641 tttcatcacc ggcataggtt ctccaacctt caccaagcat gatgaggttt ggattgaggg
    26701 cgcgtgcagc cttgtaagct tcttcgatag aagcggcgtc atggtctccc atcatatcga
    26761 aacggaatcc atccactttg taggtatcaa ctaggtattt gatagagtca actaggagcc
    26821 gtttggtcat atggtgggtt gttcccaagc gtccaccacc aaagctagtt cgaggtgtgc
    26881 catcggcatc cataaagtgg tagtagtttg gctctaggtc ttcaaagata tcgactttgg
    26941 ctgtgtggtt ataaacgaca tctaggatag ctcccatacc acgtttgtgg atttcgttga
    27001 tgaggttttt aaattctgcg attcgttttt ctggattctt aggatcgctt gagtacatac
    27061 cagtcaagga gaagtagttt tgagggtcat atccccagtt gtagttgctg ttgcttgaag
    27121 cgtagtcaga caagcgttca tggtttttca attcattgac aaagtagtaa gacaagactg
    27181 gaaggagctg gatatgggtt acacccaagt ctttgagata gtctagtttt tcaatgaagg
    27241 cttcaaaagt cccaaatggt ttggtcaagt cttttgcaat ggcaggatct gaagtgaagt
    27301 cacgcacatg agcttcgtag ataacggcgt cttcacgagt cttgaaattg tgaatcttac
    27361 cataagtcaa gtcttgaggt ccgagtttag ctggatctac aaaggcggct ttagccactt
    27421 tatgggtatc gtcaattttg gcatcgtcgc tattccaagc agcaagagat ttagcgtaag
    27481 gatcgagtgc aagaacagtt ttaccttgac gctcgatttg gtattgataa tagtagccag
    27541 tgaaatctgt gattccgagt ttgtttgtgc tgtctagagt ttgtttccaa gttcctcttt
    27601 cccctttttc gagagcgaca gttccaacta ctttgtcagg gtcattcttg tcgtagacaa
    27661 caacagaaac cttatcagca cttggtgacc aaagggtcaa atcaacttgt tttccttctt
    27721 cttttaggtc agctcccagt ttgccatcat agctgtatgt ctcatctttc aggtgccagc
    27781 ttgttttggt agtgaattgg tcggaattgt agctaacagt ataagggtgt tttgtgtcag
    27841 agaaatctcc gctgtaggtc actttcttac cagcttcatc gattgcaaca tcggtaatag
    27901 ttaccttgtt tcctaggtga ttagtgatgt tggagtgttt gaggatatct tcttttttag
    27961 caccgacaag tgttgaaaag ctactttcaa tgctagaagt gcctacgtgt tgggctcctg
    28021 tcatacggat atcatggaca tagtatggat ttgtgtaaat cgattcatca tcgtctttta
    28081 ggaaaatttg gctatgattt ttcaaatctg tgaacttata attttcttta cggattttca
    28141 cgtcgtctcc ttgtttgctc tcatctagta ataaaaatcc aaattctctt gcggcttcat
    28201 taagaggaat gtcgatatag cggccatatt tgcctgtagc cgtaaagtct gttccgtcag
    28261 gccattgagc gctacttgga tttttcacat ctccccagta ccagagagat ttcttgtcat
    28321 agttgccatc tgtgcggtag tagttgacgc gaacagttcc tgcaggctgt ggctcgtaag
    28381 agaaaacctt gtaatcttgg tctaaccaag cttcgttcat ttttggaact agtttttcta
    28441 cagatttatc gccggttaga ttttttccag ctgtattgtt gatgaggaag ctaattttct
    28501 tggcttgttc tccctttaat ttgacatcta ggtaatagcc gtagtcatct ttcttggcat
    28561 ccttgaagga caaagctccg tttggccagt tttcagatgg tttttcaaca tcgtcccaag
    28621 tccatagtcc ttgagcatcc ttgttttctt caggaagttt tttgacatgg atacggaagt
    28681 agttatcttc gatgggctct tctgccttag tttcagttgt tactggacta gtagaagtag
    28741 ttggctctgt tgcaggtttt tcttctgttt tctctaggga agcgtttgca tttctaggtg
    28801 aagaaatatc actttcgttc ttaccagagc cagctgctgt atcagtaaga ggttgggcaa
    28861 gggtggttgt atttctgttg ttgggttcgt tagtggttgg tgtactttcg tcggatgaga
    28921 tagttggtgt agccatggca agcaagacaa ggcttgcgcc gataaggaca gaaccagttc
    28981 catttttgag ggaacggatg ctgtagacca tttttttctc agtgtgagat ggtgtttttc
    29041 tcataatgat tctccaatca ataaatttct atatcagtat agcatgtagt aaaacaatat
    29101 gcaagcgttt tctttgattt gtgaaaaaat tcttactttt gagaagtttt ctgtactatt
    29161 ttatatatca gtataaatga cggagataaa aatgtcacat aagccaagcc gtattattag
    29221 gaagatgatg tgttcccagc tatttttatt cagaaattga tctctaacat taccaataaa
    29281 aaagaaaaat atcttgacaa gcaagggaaa attttgttac aataatagac ggtacttttt
    29341 acttttggtc tctcaagagt gtacagggac gtgctgacaa atgttgcaaa agtacacaca
    29401 gatgatatct gtcaccaagt gtatcatcac caaaaataaa aaaacacagg agaatgtaga
    29461 tgcctacaat taaccaattg gttcgcaaac cgcgtaaatc aaaagtagaa aaatctaaat
    29521 caccagcttt gaacgttggt tacaatagtc ataaaaaagt tcaaacaaac gtttcttcac
    29581 cacaaaaacg tggtgttgca actcgtgttg gaacaatgac acctaaaaaa cctaactcag
    29641 cccttcgtaa attcgctcgt gtacgtttga gcaaccttat cgaagttact gcctacatcc
    29701 caggtatcgg acacaacttg caagagcaca gcgtggtgct tcttcgcggt ggacgtgtaa
    29761 aagaccttcc aggggtacgt taccatatcg tccgtggtgc acttgatact gcaggtgtta
    29821 acgatcgtaa acaaggccgt tctaaatacg gtactaaacg tccaaaagca taaggaaagg
    29881 ggataaagag aaatgagtcg taaaaataga gctccaaaac gtgacgtatt gccagatccg
    29941 ctatacaatt cacaactagt tactcgtctt atcaaccgcg ttatgcttga tggtaaacgt
    30001 ggtactgctg cttcaatcgt ttacggtgct tttgagcaaa tcaaagaagc tactggcaac
    30061 gatgcacttg aagtatttga aacagctatg gaaaacatca tgcctgtact tgaagtacgt
    30121 gcacgtcgtg ttggtggttc taactaccaa gtcccagttg aagttcgtcc agaacgtcgt
    30181 acaacacttg gacttcgttg gttggtaaca atcgctcgtc ttcgtggtga acacacaatg
    30241 caagaccgtc ttgcaaaaga aatcttggat gctgctaaca acactggtgc agcagttaag
    30301 aaacgtgaag atactcaccg tatggctgaa gctaaccgtg cattcgcaca cttccgttgg
    30361 taagatagga tgcgaaagcg ttaagaaagt cccagagaaa atagggaatc gaagcaggtt
    30421 gcggttgcaa ccaatgagat tcatcttttt ctccagactt ttagcttgag ctcaactaaa
    30481 tcatgatgct aggcacggaa ggatgcaagg tgaaaatagg aaactgacgc agtattcaac
    30541 gaatacaagg agttttatct ttttcacgca gcatcccgtt ccagctcaca tcggctaact
    30601 aactttagcc tgggttcaaa ttagctaaat cgattagtat tagctataac tcagcttacc
    30661 atctcgtaag ttgaaaccaa caatagcatg aaaacattga gaacgggtag gtcctgccta
    30721 tccgttttta ttaaaatcgt gttataatag aatagaaatc aaaaataaat aggagaaaca
    30781 aacctcatgg cacgcgaatt ttcacttgaa aaaactcgta atatcggtat catggctcac
    30841 gtcgatgccg gtaaaacaac aactactgag cgtattcttt actacactgg taaaatccac
    30901 aaaatcggtg aaactcacga aggtgcgtca caaatggact ggatggagca agagcaagaa
    30961 cgtggtatca cgatcacatc tgctgcgaca acagctcaat ggaacaacca ccgcgtaaac
    31021 atcatcgaca caccaggaca cgtggacttc acaatcgaag tacaacgttc tcttcgtgta
    31081 ttggatggtg cggttaccgt tcttgactca caatcaggtg ttgaacctca aactgaaaca
    31141 gtttggcgtc aagcaactga gtacggagtt ccacgtatcg tatttgccaa caaaatggac
    31201 aaaatcggtg ctgacttcct ttactctgta agcacacttc acgatcgtct tcaagcaaat
    31261 gcacacccaa tccaattgcc aatcggttct gaagatgact tccgtggtat cattgacttg
    31321 atcaagatga aagctgaaat ctatactaac gaccttggta cggatatcct tgaagaagac
    31381 atcccagctg aataccttga ccaagctcaa gaataccgtg aaaaattgat tgaagcagtt
    31441 gctgaaactg acgaagaatt gatgatgaaa tacctcgaag gtgaagaaat cactaacgaa
    31501 gaattgaaag ctggtatccg taaagcgact atcaacgttg aattcttccc agtattgtgt
    31561 ggttcagcct tcaaaaacaa aggtgttcaa ttgatgcttg atgcggttat cgactacctt
    31621 ccaagtccac ttgacatccc agcaatcaaa ggtattaacc cagatacaga cgctgaagaa
    31681 actcgtccag catctgacga agagccattt gcagctcttg ccttcaagat catgactgac
    31741 ccattcgtag gtcgtttgac attcttccgt gtttactcag gtgttcttca atcaggttca
    31801 tacgtattga atacttctaa aggtaaacgt gaacgtatcg gacgtatcct tcaaatgcac
    31861 gctaacagcc gtcaagaaat cgacactgtt tactcaggtg atatcgctgc tgccgttggt
    31921 ttgaaagata ctacaactgg tgactcattg acagatgaaa aagctaaaat catccttgag
    31981 tcaatcaacg ttccagaacc agttatccaa ttgatggttg agccaaaatc taaagctgac
    32041 caagacaaga tgggtatcgc ccttcaaaaa ttggctgaag aagatccaac attccgcgtt
    32101 gaaacaaacg ttgaaactgg tgaaacagtt atctcaggta tgggtgaact tcaccttgac
    32161 gtccttgttg atcgtatgcg tcgtgagttc aaagttgaag cgaacgtagg tgctcctcaa
    32221 gtatcttacc gtgaaacatt ccgcgcttct actcaagcac gtggattctt caaacgtcag
    32281 tctggtggta aaggtcaatt cggtgatgtg tggattgaat ttactccaaa cgaagaaggt
    32341 aaaggattcg aattcgaaaa cgcaatcgtc ggtggtgtgg ttcctcgtga atttatccca
    32401 gcggttgaaa aaggtttggt agaatctatg gctaacggtg ttcttgcagg ttacccaatg
    32461 gttgacgtta aagctaagct ttatgatggt tcatatcacg atgtcgactc atctgaaact
    32521 gccttcaaga ttgcggcttc actttccctt aaagaagctg ctaaatcagc acaaccagct
    32581 atccttgaac caatgatgct tgtaacaatc actgttccag aagaaaacct tggtgatgtt
    32641 atgggtcacg taactgctcg tcgtggacgt gtagatggta tggaagcaca cggtaacagc
    32701 caaatcgttc gtgcttacgt tccacttgct gaaatgttcg gttacgcaac agttcttcgt
    32761 tctgcatctc aaggacgtgg tacattcatg atggtatttg accactacga agatgtacct
    32821 aagtcagtac aagaagaaat tattaagaaa aataaaggtg aagactaatc cgtcctcact
    32881 ctagaaggaa gtcacttagt ggcttccttt tgtctttaga aaatacctct aaatatggta
    32941 aaatagtaga agaataatgt gaggaaaatg aatgtcaaat agttttgaaa ttttgatgaa
    33001 tcaattgggg atgcctgctg aaatgagaca ggctcctgct ttagcacagg ccaatattga
    33061 gcgagttgtg gttcataaaa ttagtaaggt atgggagttt catttcgtat tttctaatat
    33121 tttaccgatt gaaatctttt tagaattaaa gaaaggtttg agcgaagaat tttctaagac
    33181 aggcaataaa gctgtttttg aaattaaggc tcggtctcaa gaattttcaa atcagctctt
    33241 gcagtcctac tatagggagg ctttctctga aggtccatgt gctagtcaag gttttaagtc
    33301 cctttatcaa aatttgcaag ttcgtgctga gggtaatcag ctatttattg aaggatctga
    33361 agcgattgat aaggaacatt ttaagaagaa tcatcttcct aatttagcca aacaacttga
    33421 aaagtttggt tttccaactt ttaactgtca agttgagaag aatgatgtcc tgacccaaga
    33481 gcaggaagag gcctttcatg ctgaaaatga gcagattgtt caagctgcca atgaggaagc
    33541 gctccgtgct atggaacaac tggagcagat ggcacctcct ccagcggaag agaaaccagt
    33601 ctttgatttt caagcgaaaa aagctgcagc taaacccaag ctggataagg cggagattac
    33661 tcctatgatc gaagtgacga cagaggaaaa tcgtctggta tttgaagggg ttgtttttga
    33721 tgtggagcaa aaagtgacta gaacaggtcg tgttttaatc aactttaaaa tgacggacta
    33781 tacttcaagt ttttctatgc aaaagtgggt taaaaacgag gaagaggccc agaagtttga
    33841 cctcatcaag aagaattctt ggctccgagt tcgagggaat gtggagatga ataacttcac
    33901 acgcgatttg actatgaacg tacaggatct gcaggaagtt gttcactatg agcggaagga
    33961 tttgatgcca gaaggtgagc gtcgggttga gtttcatgct catactaaca tgtcgactat
    34021 ggatgctttg ccagaggtcg aagagattgt tgcaacagct gctaagtggg gacacaaggc
    34081 ggttgctatc acagaccatg ggaatgtcca gtcctttcca catggctata aggcggctaa
    34141 gaaagcggga atccagctga tctatgggat agaagccaat atcgtggagg accgtgtccc
    34201 tatcgtctat aacgaagtgg agatggactt atcagaagca acctacgtgg tctttgacgt
    34261 ggaaacgacg ggactttcag ctatctataa tgacttgatt caggttgcgg cctctaagat
    34321 gtacaagggg aatgttattg ctgaatttga tgaatttatc aatcctgggc atcccttgtc
    34381 agcttttact acagagttaa ctggaattac agatgatcat gtcaaaaatg ccaaaccact
    34441 agaacaagtt ttgcaagaat tccaagaatt ttgcaaggat acggtcctag ttgcccacaa
    34501 tgctgccttt gacgttggct ttatgaatgc taattatgag cgtcatggtc ttccaaagat
    34561 tagtcagcca gttattgata cgctggagtt tgctagaaac ctctatcctg agtataaacg
    34621 ccatggtttg gggcctttga ccaagcgttt tggtgtggcc ttggaacatc accacatggc
    34681 caactacgat gcggaagcga ctggtcgtct gcttttcatc tttatcaaag aggtagcaga
    34741 aaaacatggt gtgaccgatt tagctagact caacattgat ctaatcagtc cagattctta
    34801 caaaaaagct cggatcaagc atgcgaccat ctatgtcaag aatcaggtag gtctaaaaaa
    34861 tatctttaag ctggtttcct tgtctaatac caagtatttt gaaggagtgc cacggattcc
    34921 gagaacggtt ctagatgccc atcgagaggg cttgatttta ggttcagcct gttcagaggg
    34981 tgaagttttt gacgtggtcg tttctcaagg tgtggatgcg gcggttgagg tggccaagta
    35041 ttatgacttt atcgaggtca tgccaccggc tatctatgcg cccttgattg ctaaagagca
    35101 ggtcaaggat atggaggaac tccagaccat tatcaagagt ttgatagagg ttggagaccg
    35161 ccttggcaag cctgttttgg ctacgggaaa tgttcactat atcgaaccgg aagaagagat
    35221 ttatcgtgaa attatcgtcc gtagtttggg acagggtgcg atgattaacc gaactatcgg
    35281 tcatggtgaa catgcccaac cagcaccact tccaaaggct cattttcgaa cgactaatga
    35341 gatgttggat gaatttgcct ttttgggaga ggaactggct cgtaaactgg ttattgaaaa
    35401 caccaatgcc ttggcagaaa tatttgaacc cgttgaagtc gttaagggtg acttgtatac
    35461 gcctttcatc gacaaggctg aagaaacagt tgctgagttg acctataaga aagcttttga
    35521 gatttatgga aatccgctgc cagatattgt tgatttgcgg attgaaaaag aattaacatc
    35581 catactgggg aatggatttg ctgtgattta tctggcatcg cagatgctgg tgcaacgttc
    35641 taatgaacgg ggttatttgg ttggttctcg tgggtctgtc ggatctagtt tcgttgcgac
    35701 catgattgga attacggagg tcaatcctct ctctcctcac tatgtctgtg gtcagtgtca
    35761 gtacagtgag tttatcacag atggttcgta cggttcagga tttgatatgc cccataagga
    35821 ctgtccaaac tgtggtcaca aactcagtaa aaacggacag gatattccgt ttgagacctt
    35881 ccttggtttt gatggggata aggttcctga tattgacttg aacttctcgg gagaagatca
    35941 gcctagcgcc cacttggatg tgcgtgatat ctttggtgaa gaatatgcct tccgtgcggg
    36001 aacagttggt acggtagctg ccaagactgc ctatggattt gtcaagggtt acgagcgaga
    36061 ttatggcaag ttttatcgtg atgcagaagt agaacgcctc gctcaaggag cggcgggtgt
    36121 caagcggaca acaggccaac acccgggggg aatcgttgtt attccgaact acatggatgt
    36181 ctacgatttt acgcctgtcc agtatccagc agatgatgtc acggctgaat ggcagaccac
    36241 tcactttaac ttccacgata tcgatgagaa cgtcctcaaa ctcgatgtac tgggacatga
    36301 tgatccgacc atgattcgaa aacttcagga tttgtctggt attgacccta ataaaattcc
    36361 tatggatgac gaaggcgtga tggcactctt ttctgggact gatgtgctag gggtaacacc
    36421 tgaacaaatt ggaacgccta cgggtatgtt ggggattcca gagtttggaa caaatttcgt
    36481 acgtggaatg gtagacgaaa cccatccgac aacctttgcg gaattgcttc agctgtctgg
    36541 tctgtcccac ggtactgatg tttggttggg gaatgctcag gatctgatta agcaaggaat
    36601 agcggaccta tcgactgtta tcggttgtcg ggacgacatc atggtttacc tcatgcatgc
    36661 gggtctggaa cctaagatgg cctttaccat tatggaacgg gtacgtaagg gtttgtggct
    36721 aaagatttca gaagaggaga gaaatggcta tatcgaagca atgaaggcta ataaggtgcc
    36781 agagtggtat atcgaatcct gtgggaaaat taagtacatg ttccctaagg cccatgcggc
    36841 agcctacgtt atgatggcct tgcgtgtagc ttacttcaag gttcaccatc ctatttatta
    36901 ctactgtgct tacttctcca ttcgtgctaa ggcttttgat atcaagacca tgggtgcggg
    36961 cttggaggtc atcaagcgca gaatggaaga aatctctgaa aaacggaaga acaatgaagc
    37021 ctctaatgtg gaaatcgatc tctatacaac tcttgagatt gtcaatgaga tgtgggaacg
    37081 aggtttcaag tttggtaaat tagatctcta ccgtagtcag gcgacagagt tcctcatcga
    37141 cggggatacc cttatcccac catttgtagc aatggatggt ctgggagaga acgttgccaa
    37201 gcaactggtg cgggcgcgtg aagagggaga attcctctct aaaacagaac tacgcaagcg
    37261 tggtggactc tcatcaacct tggttgaaaa gatggatgag atgggtattc ttggaaatat
    37321 gccagaggat aaccagttga gtttgtttga tgagttgttt taaaaaattg cttaataatc
    37381 tattaaaaga ggctaacgta tatccaatag atttacatta gctttctttt ttgttaaaat
    37441 agtctatgga aagagggtga gagtatgtca aagatgagta taagcatccg tctggatagt
    37501 gaggttaagg agcaggccca acaggtgttt agtaatctgg gaatggatat gacaacagct
    37561 attaatattt tccttcgtca ggcaattcaa tatcagggat taccttttga tgttagacta
    37621 gacgaaaatc ggaagttgct ccaagcgtta acggatttag accaaaatcg taatatgagc
    37681 cagtcttttg aatcagtctc agatttgatg gaggacttac gtgcttaaga ttcgttatca
    37741 taaacagttt aaaaaagatt ttaagttggc tatgaagcgt ggtttgaagg cagaattatt
    37801 agaagaagtt ttgaattttc tggttcaaga aaaagaacat cctgccagat atcgtgatca
    37861 ttcattgacg gcatccaagc attttcaagg agttcgtgaa tgccataccc agccagattg
    37921 gcttttggtt tataaagtag acaagtcgga attgatttta aatttgctga ggacaggcag
    37981 tcacagtgat ttattttaat ctattttaag ggggttctca tgaaactaag aatatttgcg
    38041 gaagataagc cggctaagaa ggtatttgaa tatcaattag aacttgctga tcgtacaatt
    38101 cttctatcga cagcactctt gtcaggtgct attgctttag caggaatctt ttctgctttg
    38161 aaagaaaaat aaaaatagaa aagagaaaac agaatggttt taccaaattt taaagaaaat
    38221 ctagaaaaat atgcgaaatt gttggttgcg aacggaatta acgtgcaacc tggtcacact
    38281 ttggctctct ctattgatgt ggagcaacgt gaattggcac atctaatcgt gaaagaagct
    38341 tatgccttgg gtgcgcatga ggtcatcgtt cagtggacag atgatgtgat taaccgtgag
    38401 aaattcctcc atgccccgat ggagcgtttg gacaatgtgc cagaatacaa gattgctgag
    38461 atgaactatc ttttggagaa caaggctagc cgtcttgggg ttcgttcatc tgatccaggt
    38521 gccttgaacg gagtggacgc tgacaagctt tcagcttctg ctaaagctat gggacttgcc
    38581 atgaagccta tgcgtatcgc aactcaatct aacaaggtta gctggactgt agcagccgct
    38641 gctggacttg agtgggctaa gaaagtcttc ccaaatgctg cgagcgacga agaagcagtt
    38701 gatttccttt gggatcaaat cttcaaaact tgtcgtgtct acgaagcaga tcctgttaag
    38761 gcttgggagg aacatgcagc cattctcaag agcaaggccg atatgcttaa taaggagcaa
    38821 ttttcagccc ttcactacac agcgccagga acagatttaa cacttggttt gccaaagaac
    38881 cacgtttggg aatcagctgg tgctgtcaat gcacagggcg aagaattctt gccaaatatg
    38941 ccgacagaag aggtcttcac agcgcctgac ttccgtcgtg cagatggtta tgtcacttct
    39001 acaaaaccgc ttagctacaa cggaaatatc attgaaggca ttaaggtgac ctttaaggat
    39061 ggacaaatcg tagatatcac tgctgagaag ggtgatcagg ttatgaaaga ccttgtcttt
    39121 gaaaatgcgg gtgcgcgtgc cttgggtgaa tgtgccttgg taccagatcc aagtccaatt
    39181 tctcagtcag gcattacctt ttttaacacc cttttcgatg aaaatgcgtc aaaccacttg
    39241 gctatcggtg cagcctatgc gactagcgtt gttgatggag cggagatgag cgaagaggag
    39301 cttgaagctg cagggcttaa ccgttcagat gttcacgtag actttatgat tggttctaac
    39361 caaatggata tcgatggtat tcgtgaggat ggaacgcggg tacctctttt ccgtaatggg
    39421 aattgggcaa attaaggaga taatatgtta ggaagtatgt tcgttggtct cctagtggga
    39481 tttttagcag gtgctatgac caatcgtgga gagcgaatgg gatgttttgg aaaaatgttt
    39541 ctcggttgga tcggagcctt tctaggtcac ttgctctttg gaacttgggg gccagtttta
    39601 tcaggaacag ctattatccc agcgatttta ggagccatga ttgttttagc tattttttgg
    39661 agacgaggaa gttaatttcc taaatctgat actcaatgaa aatcaaagag caaactagga
    39721 aactagccgc aggttgctca aagcactgct ttgaggttgc agatagaact gacgaagtca
    39781 gctaaaaaca ctgctttgag gttgcagata gaactgacga agtcagctaa aaacactgct
    39841 ttgaggttgc agatagaact gacgaagtca gctaaaaaca ctgttttgag gttgcagata
    39901 gaactgacga agtcagtaac atatatacgg caaggcgacg ttgacgcggt ttgaagagat
    39961 ttttgaagag tatgaaacga aaaggaggtt ggtcattgta ctagcctcct tttgatatga
    40021 tataatagtt ctatgagatt agataaattt ttagttgcct gtgcggtggg aagtcggact
    40081 gaggtcaaaa acttgctcaa ggctgggcgc gtgactgtaa atggtaaaaa agaaaaatca
    40141 gctaaattgc agattgatga aaaaatagat gagattcgct ttgatgggca agtgttggag
    40201 tatgaagagt ttgtctacta catgatgaac aagcccaaag gagttatctc agcgactgag
    40261 gatcccaagc acagaaccgt tctggacttg ttggatgact tggcgcggag taaggaagtt
    40321 tttccagtag gacgcttgga tattgacacg catggtcttt tgctcttgac caataatggt
    40381 cagctggctc atgttcttct ttcgcccaag cgtcatgtgg acaagactta tcaggctcag
    40441 gtcaagggaa ttatgaccca agaagatgtg gagacattta tcaagggcat tccgctcaaa
    40501 gactttacct gtcagcccgc tagactggag attctttcca cagatgcaga aaagaatcag
    40561 agccaaatcc gtgtgaccat tgcagaaggg aagtttcatc agatcaagcg tatggtgggc
    40621 tactgtggca aggaagtggt ggacttgcaa cgattgacta tgggaacttt agtattagat
    40681 gagaacctgg aacgagggga atggcgtcgc ttgaccaagg aagaattaga aattctccgc
    40741 gctaatatta tttaatttag tttaatactc aatgaaaatc aaagagcaaa ctaggaaact
    40801 agccgcaggc tgtacttgag tacggcaagg cgacgttgac gtggtttgaa gagattttcg
    40861 aagagtataa attcgaaaaa ggtgagggag aatgtcctta ccttttatgt tttttagttg
    40921 cttcctttgt gaaaagagtt ataatagact gtagaataaa aggaggaatc tatgaacgcg
    40981 attcaagaat catttactga taaactattt gccaactatg aagcaaatgt aaaataccaa
    41041 gcgattgaaa atgctgccag ccacaacgga atttttgcag ctctggaacg tcgccaaagc
    41101 catgtagaca acacacctgt tttctcattg gatttaacca aggacaaggt cactaaccag
    41161 aaagcgtctg gtcgttgctg gatgtttgcg gctctcaaca ccttccgcca caaactcatc
    41221 tcgcaataca aattggagaa ctttgagttg tcacaagccc acactttctt ctgggataag
    41281 tatgagaaat caaactggtt cttggagcaa gtcattgcga cttcagacca agaattgact
    41341 agccgcaagg ttagcttctt actccaaaca cctcaacaag atggtggtca atgggatatg
    41401 gtcgtttccc tctttgaaaa atacggtgtc gtgcctaagt cagtttatcc tgagtctgtt
    41461 tcatctagca gcagtcgtga gctaaatgcg atccttaata aattgcttcg tcaagatgct
    41521 caaatcttgc gtgatttgct tgtttctggt gcagatcaag tgactgttca agctaagaaa
    41581 gaagacctct tgcaagaaat ctttaacttt cttgctatgt cattaggact tccaccacgc
    41641 aagtttgact ttgcttatcg cgataaagat aacaactaca aaagtgaaaa aggaatcaca
    41701 ccacaagagt tctacaagaa atatgtcaat cttcctttag aagactacgt ttctgttatc
    41761 aatgctccaa ctgctgataa accttacgga aaatcttaca cagttgagat gttggggaat
    41821 gtggttggta gccgtgcagt tcgttacatc aacgttccaa tggagcgctt gaaagaattg
    41881 gcgattgccc aaatgcaagc aggtgagact gtttggtttg gttctgatgt cggccagctc
    41941 agtaaccgta aggctggaat ccttgcgaca gatgtttatg actttgaatc aagcatggac
    42001 attaaactta ctcaagacaa ggctggacgt ttggactata gcgaaagctt gatgacccac
    42061 gccatggtct tgacaggtgt tgacttggac gaaaatggta aatcaaccaa gtggaaggtt
    42121 gaaaactcat ggggagacaa ggtcggtaca gatggttact ttgttgcctc agacgcttgg
    42181 atggacgaat acacatacca aatcgttgtt cgtaaggaat tgctgacagc agaagaacaa
    42241 gctgcctatg gagcagaacc aattgtactt gcaccatggg atccaatggg agccttggct
    42301 aaataaaagc atagaaaaaa ggaatcagat tttagaacct ggttcctttt tagttgcttg
    42361 attacatgat gtgaagaaca tgtgccacaa tacccactgc gaagagggca aggataatag
    42421 tgattggaga tacttttttc ttaagtaagt acatgcaaag taaagtaagg agtagtcctg
    42481 ataatccagg aatcaacata tccaagtttt gttggaaagt agtaactttt tcaggagttt
    42541 gagacaatcc ttgtcctact tgtgcgaatg cttcttggat acctttagac ccttctggca
    42601 atttatccca atggatataa gccttttcat ctagttgaac tttagaaaca tcgaaagcaa
    42661 atttaatatt tacccagcgt tgaacaagga cagcaagaat gaacatccca aggatagaag
    42721 ctcctttagt gatatcttga aggataccac cagacatatc tttagtgatt tctgatccag
    42781 ccttgtatcc aatctcttga acataccaca agaatgacat acgaatcaag ttccatgcaa
    42841 caaagaagag gagtggcccc aagatattgc cagtaagggc aagtgaagca ccgagagatc
    42901 caaggattgg gcgtactgta aaccagaata ctgggtcacc gataccagca agaggtccca
    42961 tcataccgat tttaacccct tggatagcag cgtcatcgat ttccacaccg ttagcacgtt
    43021 cttcttcaag cgcaagagta acccccatga ctggagcagc tacgtatgga tgagtgttga
    43081 agaactcaag gtgacgctca agagcagcga tttgatcttc ttttttagta tagagttttt
    43141 tgatagctgg aatgagtgta taagcccagc ccaagttttg catccgttca aagttccaag
    43201 acccttgtaa gaaggttgaa cgccaccaaa cttttttacg atctgattta gttaattgaa
    43261 gtttttcagt catgatgttt tcagtccttt cttatcttag tagtcttcta ggatatcgcc
    43321 gattgggtcg ttagaagttg cggctcctcc gccaccattt ccaccagttt tagaaaggtg
    43381 aaggtagata agagcgatag caacaccgat agcaccgaat ccgattagag taatatctga
    43441 cacagcagcg agaacgaaac caagagcgaa gaatggccat acttcacgag ttgccatcat
    43501 gttgataacc atggcgtaac caacggcaac gaccatacca ccaccgatag ccataccatc
    43561 tttgagccag tctggcatgg cactaaggat actttgtaca gtttcagttg gtaccataag
    43621 gagaagagct gcaggaagcg cgatacgaag tccttggaag agtagcgcaa tgaaatgcgc
    43681 acgctccaca gcgccgaagt cacctttttt agcggcagca tctgcagtat gaaccaaacc
    43741 aactgaaatt gtacgaacaa tcattgtcaa gaaaagtcca gctacggcaa gagggatagc
    43801 aaccgcttgg gcaacaccga taccagtctt ggtaaagtca ccaccaagaa ccatgataat
    43861 ggcagcagcg acagaagcaa gtgcagcatc aggagcgata gcagcaccga tatttgacca
    43921 accaagggca atcatttgaa gcgatccacc gaggataatc cctgcttcca agtgacctgt
    43981 tacaagccca ataagggtac aggctacaag tggttggtga aattggaact ggtcgaggat
    44041 gccttcaaga cctgcaaaga aggctacaac gactactaaa accatagaaa taatagacat
    44101 gtttaaaatc ctttcataaa taatggctta tttgacattg gctttgttaa tcaagtcaaa
    44161 caaatctttt ttagaatcat ttggtacttt acggacatca aattcaacac ccaagtcacg
    44221 cattttttca aatgtagcaa catcttcttt gtccatagac aaaacggtat tgaccaatgt
    44281 tttacctgtt gagtgagcca tagaaccaac gttaagagtc ttgattggca cgccgccttc
    44341 gatggcacga agggcatctt gaggtgtttc aaacaagata agggcatgtg tttctccaaa
    44401 acgtgggtct tttgaaatct caatcagttt ttgaattgga accacgttag ccttgacatt
    44461 acctggagct gcttgtttaa tcaattcttt acgaaggtcg tctttagcca cgttatctga
    44521 agcaacgatg atacgatttg cttttgaatc tggagtccaa gcagttgcaa cctgaccgtg
    44581 aagtagacgt gtgtcaagac gggcaagatt gattttcaat ttaccgtctc cgataacagt
    44641 tccttctggg atagcagttt gggcaactgg agcagctgca gcgcttgcaa cttcttcaac
    44701 tggatttagc tcttctggaa gagctttgat gccatctttg gcttctttaa tgatattagc
    44761 agcgactttt tctacacctg cagcagcgtc catgaggcgc tctgtgtagg cttgaatcaa
    44821 catcggtaag ttaagtcctg tgatgatggc aaacttacgc tcaggatttt ctcccatcac
    44881 gcgactagct tggttaaatg gagaaccact ccaaaggtca gccaaaacta gaacctcatc
    44941 ttctgcgtca aatgcagcaa cagcgttatt aaacttagcg tatagatcat caggaccttc
    45001 atttggcata aaggtcacaa cttgaacctt ttcttgttca ccaaagatca tagatcctga
    45061 ctgatgaata cccgcagcaa attcgccgtg gctcgcaata atgattccga tactcattat
    45121 tgtcattcct ccttttttgt tttagttttc atttaagaaa actttaagac tgtttaagta
    45181 taaaccgttt tcataaaaaa agcaactatt tatcataaaa aattaaaaaa tttttatttt
    45241 tgtatgttga tatgttgagt ttttgaggag taatcttgaa ttcttattta aaatttaata
    45301 taaaaaagtt ggaagaagct tccaactttt aaaataggtt tctattagat tagtgggtga
    45361 agtcgagtac catacgtcct tggatttggc ctttttccat ttcgtcgaaa atggcaacag
    45421 catcttctac tggacgtttt tgaacaactg ggactaccag accttctgca ccaaattgga
    45481 aggcttcttc taagtcttta cgagttccga caagagaacc gatgacttgg attccatcga
    45541 ggactgtttt aacgatgctg agttccatca tttcagaagg aagaccaaca gcgacgacgc
    45601 gaccaccagc acgaatggag tcaacagcct ggttgaaggc aactttagac acagcagtta
    45661 cgacagctga atgagctcct ccatcagttt tttctttaat gagtccagct acatcttcaa
    45721 cttcgaggcc gttaatcaca atgtcagcgc ctacttcttt tgcaagggca agtttgtcat
    45781 tgttgatatc gactgcgata acatgagcat tgaatacttt tttagcgtat tgaacagcga
    45841 ggttaccaag tccaccagca ccgtaaagaa cagcccattg gcctggttca acttttgctt
    45901 ctttgatagc tttataggtt gttactccag cacatgtgat agaagaagct tgggctggat
    45961 caagtccgtc aggaactttg acagcatagt cagcagttac gatacattgt tcagccatac
    46021 caccgtctac tgagtagcca gcatttttca ctgtacggca aagggtttcg cgaccagttg
    46081 tacagtattc gcaagtgcca catccttcaa agaaccaagc aacgctgacg cggtcaccga
    46141 ctttaaggct tttcacatct ggagcaatct ctttaacgat accgatacct tcgtgcccaa
    46201 gaacacgtcc tgggacttga ccaaagtcac catgagcaac gtggaggtcg gtgtggcaaa
    46261 cgccacagta ttcaacttct acaagtgctt ccccagtttc aagtggacgg agtacttttt
    46321 cttcaatagc aacaccagtg ctttctggat ttacaacaac agctttcata gctatcctcc
    46381 ttgatattga ccgagaacag ggacaacggg actggattga ttttttgtca acagagtcga
    46441 gtgtgacgag ctctcttgta tttatatgtg aagtatatca caaaagaaag ccttttccaa
    46501 ggttttggag acaaaaagta gaacaatcga ttaactggtt gatgagactg tgaaaagagc
    46561 acaaagacca gcttgcaagc tggtcatact cttcgaaaat ctcttcaaac cacgtcagct
    46621 tcatctacaa cctcaaagca gtgctttgag caacctacgg ctagcttcct agtttgctct
    46681 ttgattctca ttgagtatca gtataggtta taagaataag ttataagaat aagtcttgta
    46741 gggttttggc aaccccatct tggtcgttgg taagggaaat ttgctcatct gcataaggga
    46801 gtagctctgg attggcattt ttcatggcat aacccttccc agcaaaagcg agcatttcgg
    46861 tatcattgtg ttcatctcca aaggcaatca aatctttttt gtcacgattc attatcttga
    46921 gcaagtagtc caaagcaaag gccttgttga caccttttgg ggtacattca aggatattga
    46981 gcggacctcc ccaggtattg atagaaagtt gatgctggta gaaggcgttc atttcttttg
    47041 ccaaggcata tttgtcactg gctctggtct gcaagaggat acagttaggg tccttggtca
    47101 ccaattcagg ctggaattga tcttcaggct ggaaagcttc tacaccaaat agtttgggat
    47161 tggcaatttc ttcattggga tttgtaatgt agaatttttt acgatattct ccagcgataa
    47221 aatcggcttg aatgtcctct gaacgttgaa ccatatctag cagatatttt ttgtctacag
    47281 tcaaacactt ttcaaaatcc caaacttggt ctggtaaatg agtaagggat ccgttgaagt
    47341 taatcatagg agtgtctaag cccagttcac ggtaaaaatc ttttgacata cggtaagggc
    47401 gacctgtcgt aataataacc tgatggcctt tttcagcaac ttttttgatg gtttttttgg
    47461 taaagtcaga aatttgactg tctgagttga gcagggttcc atctaggtca actgcaataa
    47521 tttttttcgt catatggacc tttctagtgt gttttcagat aagatgtctt ctattataac
    47581 agaaagcagg cagaaaagca gattccaaac aaaaaatgaa attggattaa tttcttaaac
    47641 aaatcaaaca aagacattga aaaagtgaat gataaatgat ataattctat tattgttcgt
    47701 aaaaattaaa aggagattga tgatggacaa attatttaaa ctaaaagaga acggtacaga
    47761 cgttcgtaca gaggttctcg ctggtttaac aactttcttt gcaatgagct atattctctt
    47821 tgtaaaccca caaattcttt cgcaaacagg aatgcctgct cagggtgtgt ttcttgcaac
    47881 gattatcggt gcagttgctg gtaccttgat gatggccttc tatgctaact tgccatacgc
    47941 tcaagcacca ggtatgggac tcaatgcctt ctttaccttt acagtcgtat ttggacttgg
    48001 ttattcatgg caagaagccc tagctatggt cttcatctgt gggattattt cattggttat
    48061 taccttgaca aatgttcgta aaatgatcat tgaatcgatt ccaaatgccc ttcgctcggc
    48121 tatttcagct ggtatcggtg tcttccttgc ctatgtaggg attaagaatg ctggactttt
    48181 gaaatttacg attgatccag gcaactatac tgttgtagga gaaggggctg acaaagctca
    48241 agcaacgatt gcagcaaact cttcagcagt tccaggcttg gtcagcttta ataatccagc
    48301 tgttttagtg gctcttgcag gacttgccat tactatcttc tttgtcatca aagggattaa
    48361 agggggaatt attctctcta tcttgacaac aactgttctt gctattgcag ttggtttggt
    48421 tgatttgtct agtatcgatt ttgctaataa ccatgttggt gcagcttttg aagatttgaa
    48481 gacaatcttt ggtgcagctc ttggttcaga aggtttggga gctttggttt cagatacagc
    48541 tcgcttgcct gaaactctga tggccattct tgccttctca ttgacagata tttttgacac
    48601 aattggtacc ttgatcggta caggtgaaaa agttggtatt gtagcgacaa atggtgaaaa
    48661 tcaccaatca gccaaattgg ataaggctct ttactctgat ttgattggaa cgacagtcgg
    48721 tgccattgca ggtacttcaa acgtaacgac ttatgttgag tctgctgctg gtatcggtgc
    48781 aggtggacgt actggtttga cagccttggt tgtagctatc tgttttgcga tttcaagctt
    48841 ctttagccca cttctagcga tcgtaccaac agcggctaca gctccaatct tgattatcgt
    48901 tgggattatg atgcttggta gcttgaaaaa tatccattgg gatgatatgt ctgaagcagt
    48961 tcctgccttc ttcacatcta tctttatggg attcagctac tctatcactc aagggattgc
    49021 agttggtttc ttgacttaca ctttgactaa gcttgtcaaa ggtcaagtta aagatgttca
    49081 tgccatgatt tggattttgg atgccttgtt tatccttaac tacatcagca tggccttata
    49141 atagaatgac ccagggggat ttcccccctt ttttaataca aaggagatag gtgatgaaag
    49201 agaaaaatat gtggaaagaa ttgttgaatc gtgcaggctg gattttggtc tttttacttg
    49261 ccgtcctttt atatcaggtt cccctagtgg ttacctctat tttgacttta aaagaagtag
    49321 ccctgctaca gtcagggctg atagttactg gcctttcaat tgtggttctg gctctattta
    49381 ttatgggagc tcgtaaaacc aagttagcta gttttaattt ttcttttttt agagctaaag
    49441 atttggcacg tttgggcttg agttatttag ttattgtcgg gtcaaatata cttggttcta
    49501 tcttgttgca actgtcaaat gagacgacaa cagctaacca gtctcagatt aacgatatgg
    49561 ttcagaatag ttctctgatt tctagcttct tcttgctagc cttgcttgct ccaatttgtg
    49621 aggaaatctt gtgccgtggg attgttccta aaaagatttt ccgaggcaag gagaacttgg
    49681 gatttgtagt cggtacgatt gtgtttgctt tattgcatca accaagtaat ttaccttctt
    49741 tattgattta tggaggtatg tcgacagttc tatcttggac ggcctacaag acccaacgtt
    49801 tggaaatgtc gatcttgctt cacatgattg ttaatgggat tgctttctgt ttgttggctc
    49861 ttgtggtgat tatgagtcgg acattaggaa tttctgttta aaagttttta tgtaggaacc
    49921 gacctctttc tactagggaa agatgaatgc aatcgtgtcc atctttttct ttttatggta
    49981 aaatagaaaa ataatatgat gaaaatcctt gagggagtga ccgatatgtc aagtaaagcc
    50041 aatcatgcaa agacagttat ttgcggaatt atcaatgtaa ccccagactc cttttcggac
    50101 ggtggtcaat tttttgctct tgagcaggca ctccagcagg ctcgtaaatt gatagcagaa
    50161 ggagccagta tgctcgatat cggcggagaa tcgactcggc cgggaagtag ctatgttgag
    50221 atagaagagg aaatccagcg tgttgttcca gtgatcaaag cgattcgcaa ggaaagtgat
    50281 gtcctcatct ctattgatac ttggaagagt caagtagcag aggctgcttt ggctactggt
    50341 gccgatctag tcaatgatat cactggtctc atgggtgatg aaaaaatggc ccatgtggtt
    50401 gctaaagctg gagcgaaagt ggtcatcatg tttaacccag ttatggctcg acctcagcat
    50461 cctagttcgc ttatcttccc tcattttggc tttggtcaaa cctttacaga aaaagagtta
    50521 gctgactttg aaacattgcc aatcgaagac ttgatggtgg ctttctttga acgagcacta
    50581 gcgagagcgg cagaagctgg tattgcacca gaaaatatcc tgttggatcc aggaattggc
    50641 tttggtctga ccaagaaaga aaatcttctc cttttacggg acctggataa actacatcag
    50701 aagggctatc caatctttct cggagtgtcg cgcaagcgat ttgtcatcaa tatccttgaa
    50761 gaaagtgggt ttgaagtcaa tcctgagaca gagcttggtt tccgaaatcg ggacacggct
    50821 tcggctcatg taactagtat cgctgcgaga cagggtgtag aagtggtgcg cgtgcatgac
    50881 gtagctagtc acaggatggc agttgaaatt gcctctgcca ttcgtctggc tgatgaagcg
    50941 gaaaatttag atttaaaaca atataaataa gatgaaagaa attgaaaaca atcagtggat
    51001 tgctaactac cggacggatc aaccgcattt tggcttggaa cgaatggtgg aactgttagc
    51061 tttgcgtggc aatccccatc ttaaactcaa ggttctccat atcggaggga ctaacggcaa
    51121 gggctcgact attgcttttt tgaaaaagat gctagaaaag ctagggctga gagttggcgt
    51181 gtttagttcg ccctatctca ttcattacac agaccagatt agcatcaatg gggaatcgat
    51241 ctcagaagcg aggctagaag ctctcatggc agactatcag tctttgctgg agggagaagc
    51301 ggtcgccaat ttacagggca caaccgagtt tgagattatc acagccctag cctatgacta
    51361 ctttgcctca gagcaagtag atgtggctat catggaagtt ggcatgggtg gacttttgga
    51421 tagtaccaat gtctgtcagc ccattttgac aggaattaca actattggct tggatcatgt
    51481 ggccctactt ggtgacacct tggaggccat agcagagcag aaggcaggta ttatcaaaca
    51541 agggatgccc ttggtaacag ggcgtattgc tccagaagcc ttgactgtga ttgaccgcat
    51601 tgcggaaggg aaagatgcgc cgagacttgc ctacgggaca gattatcagg ttcgtcatca
    51661 agaaagtgtg gtgacagggg aagtctttga ctatacaagt gctgtcagac aaggtcgctt
    51721 ccagactagc ctgcttggtt tgtaccaaat agagaatgct gggatggcca tagctttact
    51781 tgatactttt tgtcaagaag atggtcgaga gctagcaagc aatgactttc ttggtcaagc
    51841 cttggaagaa acaagttggc cagggcgttt ggaaatcgtg tcaagagatc ccttgatgat
    51901 tttggatgga gcccataatc cccatgctat caaggccttg ttggtaacct tgcaagaacg
    51961 ttttgcggat tatcataagg aaatcctctt cacttgtatc aaaaccaaag ccttggggga
    52021 tatgttggac ttgctgggag ccatgccaga taccgagctt actctaacac attttgcgga
    52081 tagtcgggcg acggatgaaa gcgtgctgaa agaggcagct aagtctagaa atctcagcta
    52141 ccaagattgg catgattttc tagagcagaa tttgacagat aaaaaagaag agaaacaaac
    52201 agttaggatt gtcacaggtt ccttgtattt cttgagccaa gtgagggcct atctgatgga
    52261 gaggaagaac gagaatggat acacaaaaga ttgaagcagc tgtaaaaatg attatcgagg
    52321 ctgtaggaga gaacgctaat cgcgagggct tgcaggaaac acctgctcgt gtagcccgta
    52381 tgtatcaaga gattttttca ggtcttggtc aaacagcaga ggaacatttg tcaaaatcct
    52441 ttgaaattat tgacgataat atggtggtag aaaaggatat ctttttccat accatgtgtg
    52501 aacaccactt cttgccattt tatggtagag cgcacattgc ctacattcca gatggtcgtg
    52561 tggcaggctt gtctaagcta gcccgtacgg ttgaagttta ttcgaaaaaa ccacaaattc
    52621 aagaacgttt gaatatcgaa gtggccgatg ccttgatgga ctatctaggt gctaaaggag
    52681 cctttgttgt cattgaggcg gaacatatgt gtatgagtat gcgtggtgtt agaaaaccag
    52741 gcactgcaac cttgacgaca gtagctcgtg gtctatttga aacagataag gacctccgcg
    52801 accaagctta tcgtttaatg gggctataaa aagaatccgt ttcaagcgga tttttctaga
    52861 aaggaatcat tatggatcaa ctgcagatta aggatttgga aatgtttgcc tatcatggtc
    52921 tttttcctag tgagaaagaa ttggggcaga aatttgtcgt ttcagccatc ctatcctatg
    52981 atatgaccaa ggcagctaca gacttggatt taacagcctc tgtccattac ggagaattgt
    53041 gtcagcagtg gacgacttgg tttcaggaaa cgagtgaaga tttgattgaa acggtagcct
    53101 ataaactggt ggaacgtacc tttgagtctt atcctcttgt ccaagaaatg aagttggaac
    53161 tgaaaaaacc ttgggcaccg gtgcatttgt cactagatac ttgctcggta accattcatc
    53221 gccgcaagca acgagccttt atcgccctag gaagcaatat gggagataaa caagcaaact
    53281 tgaagcaagc cattgacaaa ctgcgagctc gtggcatcta tattctcaaa gagtccagtg
    53341 tcttagcgac ggagccttgg ggtggagtgg agcaggatag ctttgccaat caagtggttg
    53401 aggtggaaac ctggctacca gcacaagact tgttagaaac cttgttagcc attgagtcag
    53461 agctgggacg ggtgagagaa gtgcattggg gacctcgttt gattgatttg gacttgctct
    53521 ttgtggagga ccagatcctt tatacagacg acctcatatt gcctcatcct tacatagcgg
    53581 aacgcctttt tgtccttgag tccttacagg aaattgcgcc tcattttatc catccgatat
    53641 taaaacaacc gatccgtaac ttgtatgatg ctttgaaaaa atagaaaaac tctagttttc
    53701 agttacttgc aactgaaggc tagagttttt aaaataggtc attttcttct gggaggagga
    53761 tagtttctct accgtccatg tctaaaacca gtactcttgg gggataacga gggtcgaaag
    53821 gatggttaaa gtcaaaatca atggctgtag ggaggtgttg acttgaaaag tggaaggtaa
    53881 tctttccttg gttattaagc aattgaaact cgagttcttc ttccaattca aagacatttt
    53941 ttaagaaatg gtcgataata taccaaaaag agtcaatgat gtcatcaggc aagctggtaa
    54001 caatgccaaa actagcagac cgcatgtggg tattggtaaa agccatatct ctgtctcctt
    54061 tcttttccct tatcatacag caaataggat taaaaatcaa gaaaaggtga ttttttgaaa
    54121 aggattttaa ctacagggaa aaagaaggaa aaaaattccc aaaaatctac cgaagttaat
    54181 aggtaaattc ccaaattaac ttgattatat aactttcagt tattttgaga agttaccgaa
    54241 aaatattttt tatatctatt gacttttagg ggtgaaattt ggtatgatag taggcggtat
    54301 tgtttacccc atttgaaagg ccccggaacc ttccaaatac ttttcgatgg gaaggaacac
    54361 ccatcaccgt aaacaaaaat cgaactatat ataggagaaa tcatgaacaa aacaacattt
    54421 atggctaaac caggccaagt tgaacgtaaa tggtacgtag ttgacgcaac tgatgtacca
    54481 cttggacgtc tttctgcagt agttgctagc gtacttcgcg gaaaaaacaa accaacattt
    54541 acaccacaca ctgatacagg tgacttcgtg attgttatca atgctgaaaa agttaaattg
    54601 actggtaaaa aagcaactga taaaatctac tacactcact caaaccaccc aggtggattg
    54661 aaacaaatct ctgcaggtga acttcgttct aaaaatgcag tacgtttgat tgagaaatca
    54721 gttaaaggta tgcttccaca caatacactt ggacgcgctc aaggtatgaa gttgaaagta
    54781 tttgttggag ctgagcacac tcacgctgca caacaaccag aagttcttga catttcagga
    54841 cttatctaag gaaaggaaca ataaagtatg tcacaagcac aatatgcagg tactggacgt
    54901 cgtaaaaacg ctgttgcacg cgttcgcctt gttccaggaa ctggtaaaat cactgttaac
    54961 aaaaaagatg ttgaagagta catcccacac gctgaccttc gtcttgtcat caaccaacca
    55021 ttcgcagtta cttcaactgt aggttcatac gacgttttcg ttaacgttgt aggtggtgga
    55081 tacgctggtc aatcaggagc tatccgtcac ggtatcgctc gtgcccttct tcaagtagac
    55141 ccagacttcc gcgattcatt gaaacgcgca ggacttctta cacgtgactc acgtaaagtt
    55201 gaacgtaaga aaccaggtct taagaaagct cgtaaagcat cacaatttag taaacgttaa
    55261 ttcgaaagaa ttactatact tatacagagc acctttcggg gtgttctttt tttatacttt
    55321 cttactaaat tggtgcaatt gacacagttg ttgcgacttt agtcgcttac aaatgtggct
    55381 gcaacctgac aaggtcagtt gcctcaaaac gttaatcaat acaattatat caacgtttca
    55441 aagcactcaa gagtttacct catgggtgct ttttttatgt tttttgaaaa agttcacctc
    55501 aaagtttacc ttattttcac cttattgttt tagagactgt aaggcaaatt caccgacggc
    55561 catagggact acattagaag tattgacata aatctgtgtt gtacctgtgt cgctatgtat
    55621 tagagcttca gaaatggctt ctaagctcat tcctgcttgt ttagcgagcg ttgctccggt
    55681 atgacgtagt ttgtgtggcg tagcgtgtgt aagctctggg tgtcgttttc tgatagtttt
    55741 catcttatta ttgagatagt cagaatgtaa aggtttattg atattgcctt tggtatctat
    55801 gtaagtaaag atgaattgtt ctggattact gatgatacca aactttgcca gttcatattt
    55861 ttgttgtttt ttccatgaag tcagaagttg aagtaagtca ttagaaatag agaaaatggg
    55921 aaatgtttcc acaataaaac gcatattatc agatggttaa tgcgttttat atttgggttt
    55981 gaggcttata aaataggata agcgtactga aaacttagat atgacaaaca aaataaaatt
    56041 tttaaaagct acatagaaac atggtataat gtatctaaag gaggtagcat atgtccttag
    56101 atatagataa agaaaagatg acaatcatgg gaatagcttt tgaaaaccga tccgttttta
    56161 agagtgtttg gtatgcttta agtacaaata tgattgaagg atggcgacct acggttagtg
    56221 atgttgaaaa attacgagat gaagctcttg ctctggggat gacttaatga agcgcaactg
    56281 ttatgaatct tatgactatg ttgatccgga taatttatat accgatccgg gttcttcagt
    56341 tctaagaaat aagcaggaag gaaaaaagca cgcgagcttg aatatcgtat gattgctagt
    56401 aagagtttga aattatttat caatcctatc ttagttaata gtttaaaagt agtttgtttc
    56461 acaactgcat aagcttagaa aagttgaaaa gtgataactc tttaataaac tcttggtttg
    56521 ttaaaagata ttttgtccac tacatttttt tcaattgact attatttgtc atagatataa
    56581 cagatagaac attgcattca gaataatgaa aaacgcatca aaaatggtgc gttctgtttt
    56641 tatgctacca taaaaggagg aaaacaaatt gaaaattttt aagggagagt tttatcgaat
    56701 ctctgtatta acagacaagc tagtaaggtt agaatactct cgaactggaa attttgagga
    56761 tagaacgaca caacttatct ataatagaga ttttggccaa gtttcgttag attatatcga
    56821 gacatcaaat gtactagata ttatgacgga ctattttcat ctgcacttta ataaaggaga
    56881 atttaacgcc gaaaatttat ttatagaatt aaaaggaaat tttgccgtat atggtagtcg
    56941 ctggtatttt ggtgaatcta ttgaaacgtt aaaaggaaca gctcggactc tggataaggc
    57001 agatggagca atctcgttag aagatggaat tattagccga aatggtatag ccttattgga
    57061 tgattctcaa ggatttattt gggatgaaca atctggttat attgagagag aaaatcaaat
    57121 tgacctgtat ttctttgcct atgggcatga ttatagagga gcaatcagag acttttacca
    57181 tttgactggt tcaacaccct tgttgccaag atatgcttta ggcaattggt ggagtagata
    57241 ttggccttat acgtcggatg aatacttgga tttaatagac agatttgaaa cagagaaaat
    57301 tccattatct atcggtgtgt tagatatgga ttggcatata actgacattc cagctcgttt
    57361 tggaagtggc tggacaggat atagttggaa tagaaactta ataccaaatc cagaacagtt
    57421 attgcaacaa cttcatgata gaaagttaaa actctcctta aatgtccatc ctgctgatgg
    57481 gatacgggct tatgaagaag cttatcctca aatcgcaaaa cgattggggt taaatgtaga
    57541 actagaagaa cctgctattt ttgatttttt taatccctct tttagggaag cctactttaa
    57601 agatgttcat tatgagttag aaaagcaggg agtagatttt tggtggattg actggcaaca
    57661 agggacacaa ggtatgctag atccactttg gcttttaaac cattatcact atcaggatag
    57721 ttgtaaaaat gcagaaggtg gtttgatttt atcaagatat gcaggtcctg gtagtcaccg
    57781 ctaccctgtt ggtttttcag gggatactat tattagttgg aattccttaa gatttcaacc
    57841 ctattttaca gcgacagcat ctaatatcgg ttatagttgg tggagtcatg atatcggtgg
    57901 acatatgttg ggggattatg acgaagagct acaaactaga tggctacagt ttggtgtttt
    57961 tagtccgatt actcgattac atagttctag aagtcctttt aatagtaaag aaccttggtt
    58021 tttttcagaa acaacatcta agattatgaa gaaatacctc cgtttgagac atcagatgat
    58081 tccctatcta tataccatga atgtaaagac acatgaggaa ggtgccccat taatcagtcc
    58141 aatctattat ttctatccag agaatgatga gagctataat gttccaaatc aatacttttt
    58201 tggaacagaa ctgatggtgg ctcccattgt agaaaagatg gatttgacat tccaatctgc
    58261 aaaagtagat gtatggttcc ctgaaggtga atggtatgac tttttttcag agaaaaaata
    58321 cacaggtggt gtgaagttaa gtgtttatag agacatctcg actacgcctg tgtttgcaaa
    58381 aagtggtgca atcattccct tggttggttc tgagataggt atgggtgttg atttacctga
    58441 agttgtagat tggtatgtat tcccaggaaa acaacattct tttgaaatgc ttgaagatca
    58501 aaatggtcaa agatataaaa caagattatc aatcgattgg gaaatgggaa tggtagagtt
    58561 agcattacaa ggagattcta gtatcgttcc aagcaataga aaacatagaa ttcattttaa
    58621 aggaacgaat gtgtctataa ttgaattgcc aaataagaat gatacagcta aatttgaatg
    58681 gaaagataat aaaaggacat ctttaaatga cgaagttttt agactactaa agacagcttc
    58741 tcttccatat gaattaaaag atagattgtt aaatcaattc atcaatgcga aaaactctca
    58801 tgacttaatg aatatcttgc atcatcagga taaggaattg agagggcgtt tgttggaaat
    58861 gatatttact agccaaaact aattgaaata attagaaagt catttgttta gtgtatatga
    58921 agattctgta tggtattcgt tatcactggt cacattgaaa atgtgaaata agcacctaca
    58981 acatagactt tacaacactt catgttttat atcatggggt gtttttctat ttaagtcaaa
    59041 gcccatcaaa aaatctcact attcatcagg ttaaaggaaa cctaatcata gtgagattgt
    59101 gaattatagt agagtttgta tcgcctcttc aatttgtttt gggtctgttt ttgaagaaaa
    59161 gcgttcaaag acttgcccat ctcgaccgat gagaaactta gagaaattcc attcgattcg
    59221 ttttcctagt gggccggatt tctggtcttt taaccagaca tagagagggt ctgcttcctt
    59281 accgttgacc ttaatcttgg caaaacgtgg gaaggtggtt tgaaaatgta ggctacagaa
    59341 ggcgttgatt tcctctgcgc tgccgggtgc ttgtcccata aactgattgc aagggaaatc
    59401 caatatttcg aagccctgag cttgatagcg ttcatagagt tcttgaagtc cctggtactg
    59461 gggcgttaaa ccacatccag tagcagtgtt gacaatcaag agaactttgc cacgatagct
    59521 atccaaggga gttgcttggt tgttttggtt caaaacggaa aaatcataaa gtgaagtcat
    59581 gagggcctct tttctttttc tacattgtaa cagtttttac ttttttcgtc gagtcggatg
    59641 gaagatattg ttgaagaata agtcttcaat atgatagaaa ccgtttacaa ataactttaa
    59701 aaatggtatg atttaattaa accggtttac ttagttgcta taaatctcta atttgagatt
    59761 tagttcctat tgattttaca atatgtttat tggagtgtat acatgcaaac aaaaacaaag
    59821 aagctcattg tgagtttgtc ttcacttgtt ttatcaggat ttttattaaa ccattatatg
    59881 acagttggag cggaagaaac gactacgaat accattcagc aaagccagaa ggaagttcag
    59941 tatcagcaaa gggatacaaa aaatttagtt gaaaatggtg attttggtca gacggaggac
    60001 ggaagcagtc cgtggacagg aagcaaagct caggggtggt cagcttgggt agaccagaag
    60061 aatagttctg cagatgcctc aactcgagtc attgaggcta agggtggggc tatcactatc
    60121 tcaagccatg agaaattaag ggcagcggtt caccgtatgg ttcctattga agctaagaaa
    60181 aagtataaac tgcgtttcaa gattaaaaca gatcataaag tcgggattgc caaggttcgt
    60241 atcattgagg aaagtggtaa ggacaagcga ttgtggaatt ctgcaacgac gtcaggaaca
    60301 aaggactggc agaccattga agcagactat agcccgactt tagatgttga taaaatcaag
    60361 ctggagttat tctatgaaac aggaactggg actgtttcct ttaaggatat tgagctggta
    60421 gaggtagcag accagctttc tgaggattct caaacagata aacagcttga ggaaaagatt
    60481 gatttaccaa ttggaaaaaa acatgttttt tctcttgcgg actatactta taaggtagaa
    60541 aatcctgacg ttgcttcagt caaaaatgga attttagaac ctcttaagga agggacaacc
    60601 aatgtcattg tcagtaaaga tggcaaggaa gtgaaaaaga ttcctttgaa gattctagcc
    60661 tctgttaagg atgcatacac agcccgtttg gatgactgga atggcatcat cgctgggaat
    60721 caatactatg attctaaaaa tgaacagatg gccaaattaa accaggaatt ggaaggaaag
    60781 gtagctgata gcctatctag tatttcaagt caggcggacc gcacctattt gtgggaaaaa
    60841 ttttcaaatt ataaaatgtc tgcaaatctg actgccactt atcggaaatt ggaggagatg
    60901 gccaagcaag tgaccaatcc ttcttctcgt tattatcaag atgaaactgt cgttcgaaca
    60961 gtcagggatt ccatggaatg gatgcataaa catgtctaca atagtgaaaa gagcattgtt
    61021 gggaactggt gggattatga aatcggtaca cctcgtgcca tcaacaatac cttgtctctg
    61081 atgaaagaat acttctctga tgaggaaatt aaaaaatata cagatgtgat tgaaaaattt
    61141 gtaccagatc ccgaacattt ccgaaagacg actgataacc catttaaggc tctaggtgga
    61201 aacttagttg atatgggaag agtaaaagta atagctggtt tactgcgtaa ggatgatcaa
    61261 gaaatttctt ctaccattcg ctcgattgag caagtgttca agttggtaga ccaaggtgaa
    61321 ggtttttatc aagatggatc ctatatagac cataccaatg ttgcctatac gggtgcttat
    61381 gggaatgttt tgattgatgg cctgtctcaa ctgttgccag tcattcaaaa gaccaagaat
    61441 ccaatcgata aagataaaat gcaaaccatg tactactgga ttgataaatc gtttgctcct
    61501 ttgctggtga atggagagct gatggatatg agtcgtggac gctcgatcag tcgtgcaaat
    61561 agcgaggggc acgtggccgc agtagaagta ctaagaggga ttcaccgaat agcggatatg
    61621 tctgaaggag aaaccaaaca acgtttgcag agtcttgtga agaccattgt tcaatcggat
    61681 agttattatg atgtctttaa gaatttgaag acttataagg atatcagttt gatgcaatcc
    61741 ttgttaagtg atgcaggagt cgcaagtgtt ccaagaacaa gttacctatc tgcctttaac
    61801 aagatggata aaacagccat gtacaatgca gagaaagggt ttggatttgg cttgtcactc
    61861 ttttccagtc gtaccttgaa ttacgaacac atgaacaagg aaaataaacg tggttggtat
    61921 acgagtgatg ggatgttcta tctttacaat ggcgatttga gtcactatag cgatggctac
    61981 tggccaacag ttaatccata taagatgcct ggtacaacag agacggatgc taagagagcg
    62041 gatagcgata caggtaaagt tttaccgtct gctttcgttg gaacgagcaa actagatgat
    62101 gccaatgcga cagcaaccat ggatttcacc aactggaatc aaacattgac tgctcataag
    62161 agctggttta tgctaaagga taagatcgcc tttttaggaa gcaatatcca aaacacttca
    62221 acagatactg ctgcaactac aattgaccag agaaaactgg aatcaagtaa tccatataaa
    62281 gtctatgtca atgataaaga agcctccctt acagaacaag aaaaggatta tcctgaaacc
    62341 caaagtgtct ttttagaatc atccgattcg aaaaagaata ttggttactt tttctttaag
    62401 aagagttcaa tcagtatgag taaggctttg caaaagggag cctggaagga tatcaatgaa
    62461 ggacagtcag acaaggaagt tgaaaatgaa tttcttacga ttagtcaggc tcataagcaa
    62521 aatggagatt cttatggcta tatgctcatc cctaacgtgg gtcgtgccac cttcaatcaa
    62581 atgataaaag agttagaaag tagcctcatc gaaaataacg aaacccttca gtctgtttat
    62641 gatgccaaac aaggagtttg gggcattgtg aaatatgatg attctgtctc tactatttcc
    62701 aaccaattcc aagttttgaa acgtggagtc tataccattc gaaaagaagg ggatgaatat
    62761 aagattgcct actataatcc tgaaacccag gaatcagctc cagatcagga agtctttaaa
    62821 aagctagagc aagcagctca gccacaagta cagaattcaa aagaaaagga aaaatctgaa
    62881 gaggaaaaga accattcgga tcaaaagaat ctccctcaga caggagaagg tcagtcaatc
    62941 ttggcaagtc tagggttctt gctacttggg gcgttttatt tattccgtag aggaaagaac
    63001 aactaatttg ttcatcatct agatgaatta catgaaactg tggaaaatag aaaaaatccg
    63061 aagaaggcca aatatcttcg gatttttctt ttaaaaaagg ataagttgat acttatcctt
    63121 tttacttcat tatgtgacta tagcgttttg aaactagaat aggacatcat aactgctaaa
    63181 agatttctat aaattcattt gattttccta atcaatttgt tcgtatccta tttcactcta
    63241 ctatattagt ccgtctcgca cctcacggag cgagacaaaa caaccacccg ctatgcgggt
    63301 gcgcgtcgaa ggttatacca aaaaaactcc aaacgcgata caataaaggt gttcaaccca
    63361 cttgtaaagc gaaaggagaa aaaatatggc acaaaaggct catagtttat ctcacacaaa
    63421 gtggaggtgt tctatcacat tgtgttcacc cctaagtata gacgaaaagt catctataat
    63481 caatatagaa gtagtttagg cgaaatattt catcgcttgt gtagttataa aggtgttgaa
    63541 atgatcgagg gtcacttaat gccagaccat gtacacatgt tagtcagtat tccactaagg
    63601 ctaagtgttt cgagtttcat gggttattta aaaggcaaaa gtgcactcat gatgtttgac
    63661 aaacacgcca atctcaagta caagtttggg aatcggcatt tctgggcgga aggttattat
    63721 gtaagtccag tagggcttaa tgaagacaca attaagaaat atagtcaaga ttaaagaaat
    63781 tatcgagtgg atgatttctt cacgagtatg aaaatttgag aactagtaaa gtatgatata
    63841 tagtaaaatg aaataagaac aggacaaaat cgatcaggac agtcaaatcg atttttaaca
    63901 atgttttaga agtagaggtg tactattcca gtttcaatct actatagcac tagataaatt
    63961 gagtgtaaaa gaatatgagg atccctttag ggatagtggt aagtaatacc aaagtctctt
    64021 aaagaggcaa gtgacgagtc aagagcaata aggcttgaac aacgtgaaag ccagcgtctt
    64081 taggcgctgg ctgatgattt gggcttatag ctctgagata aaccacccgt tagacgggta
    64141 gttatgattt tatctgagtg taacatactg ttgggcaatc tcgctgatgc ggtcaaagtt
    64201 gccttgggaa gcgagtttat tgagttcgcc accaattcca acggcatctg caccagcagc
    64261 gaaccattga gggatgttgt ttagaccgac tcctccggtt accattacgg aaacttgtgg
    64321 aatcggtgcc ttgactgcag agatatatgc tggactgaga gtactacctg ggaagagttt
    64381 gatgatttca ctaccggctt caagtgcagt cgtgatctct gtgagggtaa tacagcctgg
    64441 aatgtacggt gtgctgtaga gattgcacat tttcgcagtt tcagcatgga aagatggaga
    64501 aacaacgtaa tttgctccgg ctagaatggc atctctagca gttacggcat caagcacagt
    64561 acctgcaccg atacaaacac tctgatcgtc ctgatacaag tctacaagtt ccttgatgat
    64621 ttgtcctgca tactgattgg tataggcgat ttcaatagct ttgataccgc ccttgataca
    64681 agcaatcgag gcttgtagtc cttcttcctt tgtatttcct cgaatgacag caacaatttt
    64741 ctgttttttt agttcaataa ttgtatctga tttggtcatg taattctcct aacgaatgat
    64801 atcttgtgca tttgccagta aattttcaat actagttgcg gaagtggaga gatggtctcc
    64861 tggaagagtg catttgagag ttgcgctcgc aactgcaaag tcaatggtag tttttaggga
    64921 ggaatgatgg agtagttggt aaagggcacc agatataaag gcatccccgc taccaattcg
    64981 ttgatagact gcagttttta gttggacaga ctcttcaaat cgttcttcca gagcataggc
    65041 ttgatagaca tttttgtctt gctcatcact agagcggagg gtatggaaaa tggccttgaa
    65101 accataggct tcttttaaaa gtcgcatgcg attttccacc tcttctaggc tagcactgtc
    65161 tcttggaaac atctctagat tttggtcatc aatcatgaga ggatcaatac cgaagcaata
    65221 gtcagtaaaa cgtgcaaact tagaaaattc atacttggct tctaggactg aaatcatctt
    65281 tgttctcaga ttgagatcca ttgaaacgac aattcctcgg cgcttggctt cttccaagag
    65341 taggagaagg atcgcacgga cctcttgacc gatagctacg gtgattccac taaaatgaaa
    65401 atggctaatc ccctgaaaga gagaatccat atctagcatg tttggccgaa tctggctgat
    65461 actcgtatgc ttacgatcgt agaaaacttc actttgacga caaccaaagc cgttctccaa
    65521 atagtagagg ccgattcgat cgccaagccg acagattgaa ctggtatcga tttggtgctg
    65581 tttcaagaat gtgagaaaac gatctccaat ctcgttggca gggagtgcgg taaagacttt
    65641 cgttgagata cccagggctt gcaaattaca agcgatgtta atttctgatc cgccaaaata
    65701 agtcgaactg gcaacatgat ctccgatact ggtggcatct aatggtgaga ttcgaattaa
    65761 tggttcgcca aacagtaata ttttcgtcat ccattagttc ctttctggtt tagaatagct
    65821 ttacttggct aatacttctt tcaaataagc agcgacttca tcatcttggc agttttcaaa
    65881 gaagtattct tggaagtatt tgccagatat ggtgtctttg aggaagtctt ggtcgattgt
    65941 tttaaggatg gtcatcaaat cattgtgggt gatttgtttc acctcgttta agatacgagc
    66001 gttgcgttgt tcagggatta cacgttctct tgggtagcca ccgcccattt cttcagcaaa
    66061 taagcgttca aacatcaatt tgagggtcag ttctgccccc cagccaaatc ctttggcata
    66121 agggatagac aaggcgttac caccattgat ttgagaataa aggtaagcgt cagttgggtc
    66181 cactgctaga ccacagacaa caccagggaa gctgtttaaa gcaagcatag cccctacacc
    66241 cgtaccacag ccggtaacaa caaagtcaac tgcctttgta tttaaaagga tggcagccat
    66301 tagtccgttc tgcacataag ttaattgact ttctccttct tctccacgca taccatagtt
    66361 aaataattgg tagtcttttt tatctgtcgc ttcttttaga ctatcgtaaa taatgtgatt
    66421 cttgctagct tgactatttt cattgattaa tgcgattttc ataagatctt ctttctattt
    66481 ttactcaggt tgttttccga tgtaggctaa gataccgcca tctacatata ggatgtggcc
    66541 attgacaaaa ttgctggcat cactagcgag aaagacagca gggcccatca aatcttcagg
    66601 atttccccaa cgtgcagcag gtgtttttgc aatgatgaac tggtcaaatg ggtggcgaga
    66661 accatcttct tgcaactcac gaagaggtgc tgtttgagga gtggcaatat aacccggtcc
    66721 aattccgtta cattggatat tggctccacc gtattcagac gcaatgttgc gggtcaacat
    66781 tttcaagccc cctttagcag cagcataagc gctaactgtt tcacgtccca gttcgctcat
    66841 catcgaacaa atattgataa tctttccatg ccctttcttt atcatagaag gaataactgc
    66901 ctttgaaacg ataaatggtg cgtttaagtc aatatcgatg accttacgga aatcagcggc
    66961 gctcatttcg cacattggaa ctcggcggat aataccagcg ttattaacga ggatgtcaat
    67021 gacaccaacc tcttgttcga tttgcttgac catggcttgg ataccgtcct cgtctgtcac
    67081 gtcacagaca tatccatggg cttcgatgcc aacttcacga taagcttcaa tccctttatt
    67141 gaccagatct tgattgatat cgttaaagac aatagtagcg ccggcttcag cgtaggattt
    67201 ggcaatagca aatccaattc cataagaagc gccggtgatg agagcaattt ttccttttaa
    67261 agaaaactgc tcaattgaga atgatgtatt tgtcatttga tacttccttt caaatttgtt
    67321 aagacttctc tataaccaaa ttatattcta gta
//
LOCUS       DAVLVF010000002        66481 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVLVF010000002 DAVLVF010000000
VERSION     DAVLVF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 66481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66481
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.002"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(21..956)
                     /locus_tag="VYN66_000059"
     CDS             complement(21..956)
                     /locus_tag="VYN66_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV1494155.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLQQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(953..1813)
                     /locus_tag="VYN66_000060"
     CDS             complement(953..1813)
                     /locus_tag="VYN66_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1494156.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRAGQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            1921..4083
                     /locus_tag="VYN66_000061"
     CDS             1921..4083
                     /locus_tag="VYN66_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1494157.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHNVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4175..5434
                     /locus_tag="VYN66_000062"
     CDS             4175..5434
                     /locus_tag="VYN66_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1494158.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNAYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5448..6314
                     /locus_tag="VYN66_000063"
     CDS             5448..6314
                     /locus_tag="VYN66_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1494159.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            6328..7161
                     /locus_tag="VYN66_000064"
     CDS             6328..7161
                     /locus_tag="VYN66_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1494160.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     VFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7162..7242
                     /locus_tag="VYN66_000065"
                     /pseudo
     CDS             7162..7242
                     /locus_tag="VYN66_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7519..8961
                     /gene="gtfA"
                     /locus_tag="VYN66_000066"
     CDS             7519..8961
                     /gene="gtfA"
                     /locus_tag="VYN66_000066"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV1494161.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            9099..10292
                     /locus_tag="VYN66_000067"
     CDS             9099..10292
                     /locus_tag="VYN66_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1494162.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVFCLYIIS
                     LIFTGDMKSILQKMGEHSMLLLFLGYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            10978..12957
                     /locus_tag="VYN66_000068"
     CDS             10978..12957
                     /locus_tag="VYN66_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1494163.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKTSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13024..14520
                     /locus_tag="VYN66_000069"
     CDS             13024..14520
                     /locus_tag="VYN66_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1494164.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSISLPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14520..15446
                     /locus_tag="VYN66_000070"
     CDS             14520..15446
                     /locus_tag="VYN66_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1494165.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15455..16522
                     /locus_tag="VYN66_000071"
     CDS             15455..16522
                     /locus_tag="VYN66_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494166.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSKTHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16533..17459
                     /locus_tag="VYN66_000072"
     CDS             16533..17459
                     /locus_tag="VYN66_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494167.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            18026..18886
                     /locus_tag="VYN66_000073"
     CDS             18026..18886
                     /locus_tag="VYN66_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1494168.1"
                     /translation="MSQFITHQSLYATREGFVDELAKQGYVEGENIEIDLQNAQGEQR
                     NLKTISQQLAESSDVVLAIARPSAQSLANTTQTTPVIFSAVTDPVSAKLVESREHPGG
                     NVTGTSDQSSDAISTQINLIKKVLPKAKTIGILYTQSEPNSVVQKDEAKRLLKEKGFT
                     VVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIISLTMETVKQVSIKHKVPVFGGSTE
                     MIAVGGLYNYGTNYEELVRQTARMLIRVLKGEKPENIAVELPEKLELHTNQEMADALG
                     IDISKLEGKE"
     gene            18898..19791
                     /locus_tag="VYN66_000074"
     CDS             18898..19791
                     /locus_tag="VYN66_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1494169.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLTGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGTVFSLSLACFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIAEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLAL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            19804..20607
                     /locus_tag="VYN66_000075"
     CDS             19804..20607
                     /locus_tag="VYN66_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1494170.1"
                     /translation="MTSLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMTDLMKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(20640..21350)
                     /gene="treR"
                     /locus_tag="VYN66_000076"
     CDS             complement(20640..21350)
                     /gene="treR"
                     /locus_tag="VYN66_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV1494171.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIREQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            21535..23502
                     /gene="treP"
                     /locus_tag="VYN66_000077"
     CDS             21535..23502
                     /gene="treP"
                     /locus_tag="VYN66_000077"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV1494172.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKGLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            23680..25305
                     /gene="treC"
                     /locus_tag="VYN66_000078"
     CDS             23680..25305
                     /gene="treC"
                     /locus_tag="VYN66_000078"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV1494173.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDTSENAGFSTGTPWLKVGKSYKDINVENEIKGLIFTFY
                     QDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            25561..25809
                     /locus_tag="VYN66_000079"
     CDS             25561..25809
                     /locus_tag="VYN66_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV1494174.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            26016..26810
                     /gene="racE"
                     /locus_tag="VYN66_000080"
     CDS             26016..26810
                     /gene="racE"
                     /locus_tag="VYN66_000080"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV1494175.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            26807..27778
                     /locus_tag="VYN66_000081"
     CDS             26807..27778
                     /locus_tag="VYN66_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV1494176.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            27754..28275
                     /locus_tag="VYN66_000082"
     CDS             27754..28275
                     /locus_tag="VYN66_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1494177.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            28272..28733
                     /locus_tag="VYN66_000083"
     CDS             28272..28733
                     /locus_tag="VYN66_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1494178.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            28724..29464
                     /gene="xerD"
                     /locus_tag="VYN66_000084"
     CDS             28724..29464
                     /gene="xerD"
                     /locus_tag="VYN66_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV1494179.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            29464..30192
                     /locus_tag="VYN66_000085"
     CDS             29464..30192
                     /locus_tag="VYN66_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV1494180.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            30211..30780
                     /locus_tag="VYN66_000086"
     CDS             30211..30780
                     /locus_tag="VYN66_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV1494181.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            30767..31489
                     /locus_tag="VYN66_000087"
     CDS             30767..31489
                     /locus_tag="VYN66_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1494182.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            31489..31731
                     /gene="yidD"
                     /locus_tag="VYN66_000088"
     CDS             31489..31731
                     /gene="yidD"
                     /locus_tag="VYN66_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV1494183.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(31956..32921)
                     /locus_tag="VYN66_000089"
     CDS             complement(31956..32921)
                     /locus_tag="VYN66_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1494184.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLAEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(33017..33769)
                     /locus_tag="VYN66_000090"
     CDS             complement(33017..33769)
                     /locus_tag="VYN66_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1494185.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINIASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(33766..34722)
                     /locus_tag="VYN66_000091"
     CDS             complement(33766..34722)
                     /locus_tag="VYN66_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV1494186.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(34712..35671)
                     /locus_tag="VYN66_000092"
     CDS             complement(34712..35671)
                     /locus_tag="VYN66_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1494187.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(36094..36795)
                     /gene="galE"
                     /locus_tag="VYN66_000093"
     CDS             complement(36094..36795)
                     /gene="galE"
                     /locus_tag="VYN66_000093"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1494188.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            37196..38260
                     /gene="pepA"
                     /locus_tag="VYN66_000094"
     CDS             37196..38260
                     /gene="pepA"
                     /locus_tag="VYN66_000094"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV1494189.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            38282..38578
                     /locus_tag="VYN66_000095"
     CDS             38282..38578
                     /locus_tag="VYN66_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1494190.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            39260..39703
                     /locus_tag="VYN66_000096"
     CDS             39260..39703
                     /locus_tag="VYN66_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1494191.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            39707..40471
                     /locus_tag="VYN66_000097"
     CDS             39707..40471
                     /locus_tag="VYN66_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1494192.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTETFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVIPKVEDLTNRGKF"
     gene            40486..41214
                     /locus_tag="VYN66_000098"
     CDS             40486..41214
                     /locus_tag="VYN66_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494193.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIENGEFMVLVGPSGSGKTTMI
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLTTGYVLQAIALFPNLTVEENIALIPEM
                     KGWTKEEIAQKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            41207..42727
                     /locus_tag="VYN66_000099"
     CDS             41207..42727
                     /locus_tag="VYN66_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV1494194.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVYL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            43072..43878
                     /locus_tag="VYN66_000100"
     CDS             43072..43878
                     /locus_tag="VYN66_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV1494195.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(44044..44580)
                     /locus_tag="VYN66_000101"
     CDS             complement(44044..44580)
                     /locus_tag="VYN66_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1494196.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTGQEVVQFYLDLLIPKN"
     gene            44713..45603
                     /locus_tag="VYN66_000102"
     CDS             44713..45603
                     /locus_tag="VYN66_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV1494197.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            45847..46200
                     /locus_tag="VYN66_000103"
     CDS             45847..46200
                     /locus_tag="VYN66_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1494198.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            46201..47238
                     /locus_tag="VYN66_000104"
     CDS             46201..47238
                     /locus_tag="VYN66_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1494199.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIIIE"
     gene            complement(47285..48292)
                     /gene="galR"
                     /locus_tag="VYN66_000105"
     CDS             complement(47285..48292)
                     /gene="galR"
                     /locus_tag="VYN66_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV1494200.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGINLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            48457..49635
                     /locus_tag="VYN66_000106"
     CDS             48457..49635
                     /locus_tag="VYN66_000106"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV1494201.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLDRLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            49654..51135
                     /locus_tag="VYN66_000107"
     CDS             49654..51135
                     /locus_tag="VYN66_000107"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1494202.1"
                     /translation="MILVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLEMIEDSQTAREILGTELMDLVTPYPSQVNRDFWEAYVHSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            51205..51609
                     /locus_tag="VYN66_000108"
     CDS             51205..51609
                     /locus_tag="VYN66_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1494203.1"
                     /translation="MKDFHFDAISAFESYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            51790..52554
                     /locus_tag="VYN66_000109"
     CDS             51790..52554
                     /locus_tag="VYN66_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HEV1494204.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            52551..53012
                     /locus_tag="VYN66_000110"
     CDS             52551..53012
                     /locus_tag="VYN66_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEV1494205.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(53063..54325)
                     /locus_tag="VYN66_000111"
     CDS             complement(53063..54325)
                     /locus_tag="VYN66_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV1494206.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGNGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(54325..54906)
                     /locus_tag="VYN66_000112"
     CDS             complement(54325..54906)
                     /locus_tag="VYN66_000112"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV1494207.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     regulatory      complement(55306..55401)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(55784..56023)
                     /locus_tag="VYN66_000113"
     CDS             complement(55784..56023)
                     /locus_tag="VYN66_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1494208.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRLLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     gene            complement(56068..56166)
                     /locus_tag="VYN66_000114"
     CDS             complement(56068..56166)
                     /locus_tag="VYN66_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1494209.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEY"
     gene            complement(56178..57005)
                     /gene="xth"
                     /locus_tag="VYN66_000115"
     CDS             complement(56178..57005)
                     /gene="xth"
                     /locus_tag="VYN66_000115"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV1494210.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            57139..57595
                     /locus_tag="VYN66_000116"
                     /pseudo
     CDS             57139..57595
                     /locus_tag="VYN66_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            57592..58122
                     /locus_tag="VYN66_000117"
     CDS             57592..58122
                     /locus_tag="VYN66_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV1494211.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNID
                     PEACRSVGLSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDG
                     VIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKPGVLYSS
                     KISSNRVNVALPYVGY"
     gene            58250..59995
                     /locus_tag="VYN66_000118"
     CDS             58250..59995
                     /locus_tag="VYN66_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494212.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLVVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            59985..61736
                     /locus_tag="VYN66_000119"
     CDS             59985..61736
                     /locus_tag="VYN66_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494213.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            61909..62601
                     /locus_tag="VYN66_000120"
     CDS             61909..62601
                     /locus_tag="VYN66_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1494214.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            62644..63870
                     /locus_tag="VYN66_000121"
     CDS             62644..63870
                     /locus_tag="VYN66_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV1494215.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            65034..>66481
                     /gene="pfbA"
                     /locus_tag="VYN66_000122"
     CDS             65034..>66481
                     /gene="pfbA"
                     /locus_tag="VYN66_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV1494216.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEY"
BASE COUNT        19374 a        12327 c        14918 g        19862 t
ORIGIN      
        1 tatattataa ttgtgttatt ttacttccaa ctattgagag aaatttcgcc actatttagc
       61 cagatttctt ttccgttatc acattgaact aaaagttttc cattttctga gatgtcttta
      121 gcaagtccct tgtagtcttt ttgctgtagt gtgaaagtga cttcttttcc tagaatgaat
      181 gactgttttt tgtataggta taatagctct tctgctggtg tttcgaagaa agcacgccag
      241 atttctatga tcaattcatt ccttgttata ggagctgtag ctttaaataa gctggcagct
      301 ttttctttta attcctgagg gaagtcttta atagtgaaat tgatacctac tccaataatg
      361 atatctgtga ctaagccagt ttctacagag gtcattgctt cagtaaggat tcctccaatt
      421 ttatgattgt ttagatagat atcattgacc cattttatgt cgacatctat taaagttagg
      481 ttcttaatag ctttgtagac agctccagct acaagtagtg tgtaggatgg taatttgtca
      541 taggggagat ttggtttaag atggagtgtc atataaatac caccttgtgg tgagtagaag
      601 gaacgttgaa aacgtcctcg gcctgctgtt tgataggaag ctagatagag ggtatttgct
      661 tcatggccta aatcaattgc ttcttttgca tctagttgtg ttgattttgt ttcgggttta
      721 aagctgactt taattggaag attttcttct agaatctctg gaagaataag gtcaccattc
      781 attagtttat atcctctatt tttgatacta tcaatttcaa tgccttcttg ttctagtcgc
      841 ttgatagctt tccaaattga tgttcggctt agggatagtt tttctgcgat tttttctccg
      901 ctgatatagt cggtttcttt agataggatt tggtagacag cttggtagga tttcataatg
      961 ttgcctttct cactagttgg tattgagagt attcttttct tgtatgactt ggagtctgat
     1021 taaagtattg tttataagct ttcgaaaaat ggagtggatc tgaaaaacct accgagtatg
     1081 caattacctt gatggactct tgggtatttt cgagaagttg tccagctcgg tgcattcgaa
     1141 cgtagagtag gtattctttg ggtgataagg tattaaattc tttgaatacg cttgataagt
     1201 agcttctgtg aacggatagt tcttttgcta aatcttgaat tgtaagtgat tgaggatagt
     1261 ggctatcaat taatcgtttg cattcaagat agagttggtg ggttgatgaa atattctttt
     1321 ttttctgatt gggagcaata gttcccagat gaaacatcag ttcatgaagt tgtcccatga
     1381 tatggagttg agctaattca cttgattttg taatctgagc gaagcggaca atgtctgaga
     1441 tgagttttgc agtagtctgg gtatgacaag tttcagattg gatgagatag gattgatcag
     1501 aaatttggga aagagcaaaa taatcagggg ctttccctcc agtgattcct aaccagtagt
     1561 aggcccaagg ttctttacta tctgcttgat aaaaggttag ttcctctggt tttaatagaa
     1621 agaaatctcc ttcttttaaa tcaacaattt tacccttgta atgaaatttt ccttgtcctt
     1681 tagtaatgta atgtagaacg tatgtatcac gaatggctgg accaaaagag taattaggtg
     1741 tgcattcctc atatccataa aagcttaggg caaggtcgat tgttccagtc tggtattctg
     1801 aaaaaactaa catgtgctac ctcctaccta acattttacc atattcttga gacatttttc
     1861 tattttggaa agcgatttca ggtgataaaa tataatcaat aaagtgatga ggtgaagtaa
     1921 atgggagtta ggatagagaa taatctattt tatgttgaga gtaaaaatct aagtttgatt
     1981 attgaaaatc gaaatggcta cttacttttg aaacatttag gaaagactat taagaactat
     2041 aaaggttcca atagtgttta tgaacgagac catgcctttt caggaaatcc aacggctact
     2101 aatcgaacct ttagtttaga tactcaacgt caaatttttg gacaacatgg cttaggagat
     2161 tttaggaaac caaccataca ggttcagcat aatgtaactg aagtaacaga ctttcgattt
     2221 gtagaagcaa agattttaaa aggtcagaat ggtccacagg gcttaccttc tccacatagc
     2281 atggacgata cagagactct tgtcttaatg ttagaagatt ctaaggctca acttagtctg
     2341 actttgtatt atactacttt taataatgat gcgactattg ctagctacag taaattagat
     2401 aataatagta atcaggaagt tgtcatccat aaagattttt cttttatggc tgattttcca
     2461 gctgcagatt atgaaatagc aactctgcag ggtgcttatg ctcgtgaaaa gactgttaga
     2521 cgtcaacagg tagaacaagg aatcttttcg attagttcaa accgaggtgc ttctggtcat
     2581 gctcaaacac cagctcttct actatgcgaa caaggagtca cagaggatgc tgggaatgtg
     2641 tttgctattc aactaatgta tagtggcaac tttgaagctt ttgttcaaaa aaatcaattg
     2701 aatgaagttc gggtggctat tggcattaat ccagaaaact tttcttggaa gttagcccct
     2761 gaggaatact ttgaaacacc ggtagcttta gtgactcatt cagatcaggg attaactggt
     2821 attagtcatg aaagtcagaa ttttgtactg aagcacatta tgctaagtga attttctaaa
     2881 aaagaacgtc caattctaat caataactgg gaagctactt actttgactt tcagagagaa
     2941 aaactgttag agttagcaga tgaagctaag aaagttggca ttgaactttt tgtattagat
     3001 gatggttggt ttggcaatcg ttttgatgat aatcgtgctt taggtgattg ggttgttaat
     3061 gaggaaaaac tgggtggaag tctagaaagt ctgatttcag ctatccatga aagaggtttg
     3121 cagtttggac tttggttaga acccgaaatg atttctgtag atagtgattt gtatcgtcaa
     3181 catcctgact gggctattca ggttcctgat tatgagcata cttattctcg gaatcaatta
     3241 gtacttaatc ttgccaatcc tcaggtagta gaatacttga aaagtgtctt agatcaactc
     3301 ctatcttatc atgagattga ttacattaaa tgggatatga accgcaatat cactaagcta
     3361 gggaatggat taacttatct agagacacag atgcaatctc atcagtacat gctggggctt
     3421 tacgaactcg tttcttatct gacagagaag cacagccata ttctctttga gtcctgctct
     3481 ggtggtggtg gacgaaatga tcttggtatg atacgctatt tcccacaagt ctgggctagt
     3541 gataatactg atgccattgc acgtttacca attcaatacg gttcatccta tctctatcca
     3601 accatttcta tgggggctca tgtgtcagca gtaccgaatc atcagatggg acgaatgaca
     3661 ccattagaaa cacgtggcca tgtagcaatg atgggaaatt tgggctatga gcttgatttg
     3721 acaaatttat cagatgaaga gaaagctacg attgctaatc aggtgaactt gtataaagaa
     3781 ttacgaccag tagttcagtt aggacagcag tatagactaa ttaatcctga tactgcatcc
     3841 aatgaagcag ctgtacaatt taattacgga aatcaaacga ttgtaaccta cgttcgcgtt
     3901 ttatctgttg tagagaccat ggaaacaact ttaaaattaa aagatttgga tgaagaggga
     3961 ctatataaat tacaggaaaa tggcgaagtt tactcaggtg cagaactaat gtatgcgggc
     4021 ttaactgtta tcttatccca aggagatttt ttgagtagac agtatatttt tagaaaacta
     4081 tgatatgtac tgaccccaaa agttagattt attctgtcta acttttgggg tgcagttcaa
     4141 taagggtgta taaatttata aaggaggctt tctaatggaa tggtataaaa aaatcggact
     4201 tcttgcaact acaggtttag ctttgtttgg gctcggcgct tgctccaact atggtaaatc
     4261 tgcggatggc acagtgacca tcgagtattt caaccagaaa aaagaaatga ccaaaacctt
     4321 ggaagaaatc actcgtgatt ttgagaagga aaaccctaag atcaaggtca aagtcgtcaa
     4381 tgtaccaaat gctggtgaag tattgaagac acgcgttctc gcaggagatg tgcctgatgt
     4441 ggtcaatatt tacccacagt ccatcgaact gcaagaatgg gcaaaagcag gtgtttttga
     4501 agatttgagc aacaaagact acctgaaacg cgtgaaaaat gcctacgctg aaaaatatgc
     4561 tgtaaacgaa aaagtttata acgttccttt tacagctaat gcttatggaa tttactacaa
     4621 caaagataaa ttcgaagaac tgggcttgaa ggttcctgaa acctgggatg aatttgaaca
     4681 gttagtcaaa gatatcgttg ctaaaggaca aacaccattt ggaattgcag gtgcagatgc
     4741 ctggacactc aatggttaca atcaattagc ctttgcgaca gcaacaggtg gaggaaaaga
     4801 agcaaatcaa taccttcgtt attctcaacc aaatgccatt aaattgtcgg atccgattat
     4861 gaaagatgac atcaaggtca tggacatcct tcgcatcaat ggctctaagc aaaagaactg
     4921 ggaaggtgct ggctataccg atgttatcgg agccttcgca cgtggggatg tcctcatgac
     4981 accaaatggg tcttgggcga tcacagcgat taatgaacaa aaaccgaact ttaagattgg
     5041 gaccttcatg attccaggaa aagaaaaagg acaaagctta accgttggtg cgggagactt
     5101 ggcatggtct atctcagcca ccaccaaaca tccaaaagaa gccaatgcct ttgtggaata
     5161 tatgacccgt ccagaagtca tgcaaaaata ctacgatgtg gacggatctc caacagcgat
     5221 cgaaggggtc aaacaagcag gagaagattc accgcttgct ggtatgaccg aatatgcctt
     5281 tacggatcgt cacttggtct ggttgcaaca atactggacc agtgaagcag acttccatac
     5341 cttgaccatg aactatgtct tgaccggtga taaacaaggc atggtcaatg atttgaatgc
     5401 cttctttaac ccgatgaaag cggatgtgga ttaggagtag agagactatg aaaaaagtat
     5461 tacaaaaata ttgggcatgg gcttttgtgg tcatccccct cttgttacaa gcaattttct
     5521 tctatgtgcc gatgtttcaa ggagcctttt acagttttac caactggaca ggattgactt
     5581 acaactacaa atttgtcggc ttaaacaact ttaagctcct cttcatggat ccaaaattca
     5641 tgaatgcgat tggctttacc gcaatcatta cgattgccat ggtggttggt gagattgcac
     5701 tcgggatctt cattgcgcgt gtcttgaact ctaaaatcaa aggccaaacc ttcttccgtg
     5761 cttggttctt cttcccagct gttttatctg gtttgacagt ggctttgatc ttcaagcaag
     5821 tcttcaacta cggtcttcca gcgattggga atgcccttca tattgaattt ctccaaacca
     5881 gtcttttagg gactaagtgg ggagcaatct ttgcggctgt ctttgtcctt ctttggcaag
     5941 gggtggctat gcccatcatc atcttcctag ctggtttgca atctattcca actgagatta
     6001 cagaggcagc aaggattgat ggtgcgacta gcaagcaagt tttctggaac attgaattgc
     6061 cttacttgct accaagtgtc tctatggtct ttatcctagc cctaaaaggt gggctgactg
     6121 cctttgacca agtctttgcc atgaccggtg gtggtccaaa caatgccaca acctcacttg
     6181 ggctcttggt ttataactat gcctttaaaa acaaccaatt cggttatgcc aatgccattg
     6241 ccgtaatctt gttcctctta attgtagtga tttcgatcat ccaattgaga gtatctaaga
     6301 aatttgaaat ttaagaggag aagcatgatg aaacaagatg aaagaaaagc cctgattggc
     6361 aaatacattc tattgattct aggatcggtt ctgattttag tgccgctcct tgctaccctc
     6421 tttagttcct tcaaacccac taaggatatt gtagataatt tctttggctt tccaaccaac
     6481 ttcacatggg acaactttag ccgtctctta gctgatggga ttggaggcta ttattggaac
     6541 tctgtcgtca tcactgtctt gtctttactt gcagtaatga tctttatccc tatggcagcc
     6601 tactccatcg ctcgcaatat gagtaaaaga aaagtcttta ccatcatgta taccctctta
     6661 atcctcggaa tcttcgtacc tttccaagtc atcatgattc cgattacggt tatgatgagt
     6721 aaactcggtt tggctaatac ctttggtttg atcttgctct acttgaccta tgcgattcca
     6781 cagaccctct ttctctatgt tggctatatc aaaatctcga ttccagaaag tctggatgaa
     6841 gcagcagaga tcgatggggc taatcaattt acaacctatt tccgcatcat cttcccaatg
     6901 atgaaaccga tgcatgcgac aaccatgatc atcaatgccc tttggttctg gaatgacttc
     6961 atgttgccac tccttgtctt gaaccgggat tccaaaatgt ggactctgcc tttgttccaa
     7021 tacaactacg caggccaata tttcaacgac tacggaccaa gctttgcctc ttacgtggtc
     7081 ggcattatca gtatcaccat tgtctatctc ttcttccaac gccatatcat ttcaggaatg
     7141 agcaacgggg cagtgaagta atactcaatg aaaatcaaag agcaaactag gaagctagcc
     7201 gcaggttgct caaaacactg ttttgaggtt gtggatgaaa ctgacgaagt cagtaaccat
     7261 acctacggta aggtgaagct gacgtggttt gaagagattt tcgaagagta taagaagagc
     7321 tcatggaatc tatagtcttt gacacagagg aagagttcag cttgttgata attctatgta
     7381 gaaaacaaga aagtagtact ttgtttatca cagtctatat tagtacgagt ttgatgataa
     7441 atagagattt aaaatgaatt tgatttacga agccagatca tatgagactt ggcttcaatt
     7501 agaaaaagga gagtaatgat gccaattcaa aataaaacca tgttgattac ctattctgat
     7561 agccttggaa ataatcttaa agacttatat gataatttgg aagagcattt tggagatgct
     7621 attggaggag ttcacctttt accatttttc ccatcaacag gtgatcgtgg atttgcgcca
     7681 gttgactacg acgaagtgga ctcagctttt ggtgattggg aggatgttaa gcgtttaggt
     7741 gagaaatatt atcttatgtt tgattttatg attaatcata tttctcgtca atccaagtat
     7801 tataaggact atcaagaaaa acatgaagcc agtgaattta aagatctctt tttaaactgg
     7861 gataagtttt ggccagaaaa ccgtccgaca cagtctgatg tagatttaat ttacaagcgt
     7921 aaggatcgtg caccaaagca agagattgtg tttgaagatg gttcagtgga acatttgtgg
     7981 aatacctttg gtgaggagca gattgatctt gatgtgacca aagaagtaac tatggaattt
     8041 atccgtaaga ccattcagca cttggcaagt aatgggtgtg atttgattcg tctagatgcc
     8101 tttgcttatg cagtgaagaa attggatact aatgatttct ttgtggaacc agatatttgg
     8161 gatttattgg acaaagttcg agatatcgct gctgagtatg ggacagagct tttacctgag
     8221 attcatgaac actattcgat tcagtttaaa atagcagacc atgattacta tgtttatgat
     8281 tttgctcttc caatggtgac actttatact ctttacagtt ccagaacaga gcgtttggct
     8341 aagtggttaa agatgagccc gatgaagcaa tttacgacgc tagataccca tgatgggatt
     8401 ggagtagtag atgtcaagga tatcctgacc gatgaggaga ttgactatgc ttcaaatgaa
     8461 ctctataagg ttggagccaa tgtcaaacgt aagtactcta gtgccgagta taacaactta
     8521 gatatctacc aaatcaattc aacctactat tcagcgcttg gagatgatga tgtcaagtat
     8581 tttctcgctc gtctaattca agcttttgcc ccaggtattc ctcaggttta ctatgtgggt
     8641 ctattagcag gcaagaatga cttgaaatta ttagaagaaa ctaaagaagg tcgaaatatt
     8701 aatcgtcatt actatagcaa cgaggaaata gcaaaagaag tgcaacgacc tgttgtgaag
     8761 gcccttctca atctattttc tttccgtaac cgttcagaag cctttgatct agaagggact
     8821 actgagatag agacaccaac agctcacagc attgtaatca aacgtcaaaa taaagataag
     8881 tccgtaacag cagtagcaga aattgacttg caaaatcaga cttatcgggt aattgagaat
     8941 ggagttgaag taacattttg aagccttgat atggtggata aaatagggtt tttattttgg
     9001 aaaacgtttc ctttgttttc aaattgctaa aaaagtggta caatatagga tagcttacta
     9061 ttatctgaat cagctgattt ggagagaaag gattcatttt gaaatcaata ggctttattg
     9121 aaaagctgaa ggggttgtct agtaaagagc tgattttatt gggaattatc ctgagtatct
     9181 ttttaccctt ttatcttttt gtagttgtat tctgtttata tattatcagt ctgattttta
     9241 caggagacat gaaaagtatt cttcagaaaa tgggggagca ttcgatgctg cttctttttc
     9301 ttggctatag tactgttata tccattcttg cacaaaattg gatgggtctt gtggcttcag
     9361 taggaatgtt tctatttact attttctttt tgcactatca gtcgatttta tcccataaat
     9421 tctttcgatt gattttgcag ttcgtcttgt ttggtagtgt cttgtcagct gcttttgcca
     9481 gtttagaaca tttccaaatt gtgaagaaat ttaactatgc ttttctttcc cccaatatgc
     9541 aggtgtggca tcagaaccgg gcagaagtga ccttctttaa tcctaattat tatggaatta
     9601 tttgttgttt ctgtattatg attgctttct atctgtttac aacgaccaag ttgaattggt
     9661 tgaaagtatt ctgtgtgatt gcaggctttg ttaatctctt tggtttgaac tttactcaaa
     9721 atcgaactgc ctttcctgct attatcgctg gagcaattat ctatctcttt acgactatta
     9781 aaaactggaa ggccttttgg cttagtattg gggtcttcgc gattggtttg agtttcctct
     9841 tttctagtga tttgggagtt cgaatgggta ctttagactc ttctatggaa gaacgcattt
     9901 ctatctggga tgctgggatg gccttgttta agcaaaatcc tttttggggt gaagggccat
     9961 tgacctatat gcactcttat cctcggatac atgctcctta tcatgaacat gcccacagtc
    10021 tttatattga tacgattctg agttacggaa ttgtgggtac cattttatta gttctgtctt
    10081 ctgttgctcc tgttcgcttg atgatggata tgagtcagga gtcggggaaa cgtccgatta
    10141 tcggccttta tctatctttc cttacagtgg ttgctgtgca cggaattttt gacttggctc
    10201 tcttctggat tcagtcaggc tttattttct tgctagttat gtgcagtatt ccattggagc
    10261 atcgaatgtt ggtatcggac atgacggatt aagttttata agtttgaaat cttctacctt
    10321 gggtaggaga ttttttatac gatgggcatg tcgtgcatct gaggtgtaag tcctctgtag
    10381 ggatccacta ccggtgaata caatagcgct ccccaagcct gactatcgtg aggtagcgga
    10441 ttaaaatggt ctggggatag atcgttttaa gtctgacgca ggaaataaga attgtcagag
    10501 aaagcaatag tgaaatcgta ggctatgaat agaaacgtga tagcgaggag catatagtgg
    10561 ataaggtagc gagaaatttt aaagtataaa agatagtctt aatctatata tgtaaatcac
    10621 aaaagtaatt gaattcggaa taaggttggt gtgaaaagac atagtaaact gaatcttgaa
    10681 tactagaatg atacttctac aatattgtta taatttaatt tgaattccaa aatcacattg
    10741 ttccgtccgt atttcatttt actgtatgtc ttcctaaact ccaaagtttc tgcgtcaaat
    10801 ttcctatttt cactgtagtc tttgacactt cgaacgacaa tttgaatcat atattcaaaa
    10861 aaaaatgaaa aatttgtgcc aacaagccta aatattctga aaatttgttt gatttttaat
    10921 ggataatgtg atataatggt tgtcaatcta taattcacat aaacagagga gtagaaaatg
    10981 aaaaaaaatc gtgtatttgc tacagcaggt cttgttttat tagcagcagg tgtacttgca
    11041 gcatgcagtt cttcaaaaac atctgattca tcagccccta aagcttatgg ctatgtttat
    11101 acagcagacc cagaaacctt ggactacctg atttcaagta aaaatagtac aacagtagtg
    11161 acttcaaatg ggattgatgg tttattcact aacgataatt acggtaatct tgctcctgca
    11221 gttgcagagg attgggaagt ctctaaggat ggtttgacct acacttataa gattcgtaaa
    11281 ggggttaaat ggtttacctc tgatggagaa gaatatgcag aggtgacggc taaagatttc
    11341 gtgaacggtt taaaacacgc agcagataaa aaatcagaag ctatgtattt agctgaaaat
    11401 tcggttaaag gcttggcaga ttatctatca ggaacttcaa cagatttttc aacagttggt
    11461 gtcaaggcgg ttgatgatta tacgttacaa tacactttga accagcctga accgttctgg
    11521 aactctaagt tgacctattc tattttctgg cctctgaatg aagaattcga aacatcaaaa
    11581 ggaagcgatt ttgctaaacc aacagatccg acatccttgc tttataatgg tccattcttg
    11641 ttgaaagggt tgactgcaaa atcttctgta gagtttgtaa aaaatgagca atattgggat
    11701 aaagaaaatg tccacctaga tactatcaat ctagcttact atgatggatc agatcaggag
    11761 tcgctagagc gtaacttcac tagtggagct tatagttatg cccgtcttta ccctaccagc
    11821 tccaactatt ctaaggttgc agaagaatac aaggacaata tctattacac acaatcaggc
    11881 tctgggattg ctggtctggg tgtgaatatt gatcgccaaa gttacaacta tacttctaaa
    11941 actacagatt cagagaaagt agctactaag aaggcattgc ttaacaaaga tttccgtcaa
    12001 gccttgaatt ttgctcttga tcgctcagct tactcagctc aaatcaatgg taaagatgga
    12061 gcagctttag cagttcgtaa tttatttgta aaaccagact ttgtttcagc tggtgagaag
    12121 acctttggtg atttagtcgc tgctcaactt cctgcttatg gtgatgagtg gaaaggtgtg
    12181 aatttagctg atgggcagga tggtttattc aatgctgaca aggccaaggc agagtttgcg
    12241 aaagctaaga aagctttaga agcagacggc gttcagtttc ctattcatct ggacgttcca
    12301 gtagaccaag catcaaaaaa ctatatatct cgtattcagt cctttaaaca atctgtagaa
    12361 acagttcttg gtgttgaaaa tgtcgttgtt gatattcaac aaatgacaag tgatgaattc
    12421 cttaatatta cttactatgc tgccaatgct tcatctgagg attgggatgt atcgggagga
    12481 gtttcatggg ggccagacta tcaagaccca tctacttacc tggatatttt aaaaacaact
    12541 agcagtgaaa ctacaaaaac atatttagga tttgataatc caaatagccc ttcagtagtt
    12601 caagttggtt tgaaagaata cgataaatta gttgatgaag ctgccaaaga gacaagcgac
    12661 ttgaatgtcc gttatgaaaa atatgcagcg gctcaagcat ggttgacaga tagttcactc
    12721 tttattcctg ctatggcttc ttctggtgca gcaccagtgc tttcacgaat tgttccattt
    12781 actggagctt ctgcgcaaac aggctctaag gggtcagatg tttacttcaa atatttgaaa
    12841 ttacaagata aagcggtgac taaggaagag tatgaaaaag ctcgtgaaaa atggttgaaa
    12901 gaaaaagctg aatcaaatga gaaagctcaa aaagaattgg caagtcatgt gaagtaaata
    12961 atttctaata gaactttctc tccatgagga gaaggttctt ttgggatttt taaaggaaat
    13021 aatatgaaaa aatatatttt tatgcgtgtt ttgcggtcat tggtttcgat tttcttagta
    13081 acgactttga cctacacgat tatctatacc ttggttcctc gaaaattgat tttcaagcag
    13141 gatcctaact ataataaaat tgcgacaacg gctgataaac gtgataacta tgaaaatact
    13201 gtgtttgagc gtatgggcta cattgagtat tacgatacta aagagttgca agaaaaggca
    13261 agtagcatgg attcttctgt aacagtagaa gcaaatgcga ccaataaagc tatttatgaa
    13321 aagtacatca atcaattagg tcatggttgg actttgggag aatttactga aagtggtcaa
    13381 ttctatgcta ctcgtgaaat tccaattttt gaacgtgttt ttcacttcta tgctaacttg
    13441 attgacattg accatacaaa taaaatccaa gaccctgaaa atccagactt gaaacgctac
    13501 cttcgttttg aaaacgatcc agctatcgga tggtcattgg tcggttcagg aactaaacat
    13561 aaatatctct tgtactttaa cagtcagttc ccatttgttc atcaaaactt tgtgaacttg
    13621 aatttaggtg actcttaccc aacctatgct aatacaccag ttcttcaggt tattactcaa
    13681 ggtcaaggac aaaccaaaac tgcccaagtt cagttcccaa caggtaagaa aacgtcttct
    13741 gtaaatattt actcaagaac ctacaagtca cctagtcagg ctgactctcg tgaagtagct
    13801 agctatggga aagatgatcc ttatacagcg actgaaagta attaccaata tccatctatg
    13861 attgtcagct ctgctattac tggtttgatt ggtttggttc ttgcctatgc tcttgccgtg
    13921 ccacttggtt cagccatggc tcgtttcaag aacacttgga ttgatagcct ctcaacaggg
    13981 gctttgacct tcttgcttgc tcttccaacg attgccttgg tttacatcgt tcgattgatt
    14041 ggatcatcta tttcccttcc agattcattc cctatcttgg gagctggaga ttggcgttct
    14101 tacgttttac cagcagtcat ccttggtttg ttgggtgctc ctggtacagc catttggatt
    14161 cgtcgttaca tgattgactt gcaatctcaa gactttgttc gtttcgctcg tgcaaaaggt
    14221 ttgtctgaaa aagaaatttc aaacaaacac atctttaaaa atgccatggt tccgctggtt
    14281 tcaggaattc ctggtgccat tattggggtt atcggtggtg caacccttac tgaaacagtc
    14341 ttcgccttcc caggtatggg taaaatgttg attgactctg taaaagcatc taataactct
    14401 atggtcgttg gtcttgtctt catctttaca tgtatttcta tcttctcacg tcttttggga
    14461 gatatttgga tgactattat tgacccacgt attaaattga ctgagaaagg aggcaaataa
    14521 tgtctacaat cgataaagaa aaatttcagt ttgtaaaacg tgacgatttt gcctctgaaa
    14581 ctattgatgc gccagcatat tcttactgga aatcagtgtt taaacaattt atgaagaaaa
    14641 aatcaactgt agtcatgttg ggaatcttgg tagccatcat tttgataagt ttcatctacc
    14701 caatgttttc taagtttgat ttcaatgatg tcagcaaggt aaacgacttt agtgttcgtt
    14761 atatcaagcc aaatgcggag cattggttcg gtactgacag taacggtaaa tcgctctttg
    14821 acggtgtctg gttcggagct cgtaactcca tcctcatttc tgtgattgcg acagtgatta
    14881 acttggttat cggtgttttt gtcggtggta tttggggtat ttcaaaatca gttgaccgtg
    14941 tcatgatgga agtttacaac gtcatctcaa acatcccacc tcttttgatt gttattgtct
    15001 tgacttactc aatcggagct ggattctgga atctgatttt tgccatgagc gtaacaacat
    15061 ggattggtat tgccttcatg atccgtgtgc aaatcttgcg ctatcgtgac ttggaataca
    15121 acttggcgtc acgtactttg ggaacaccaa ccttgaagat tgttgccaaa aatatcatgc
    15181 ctcaattggt atctgttatt gtgacaacca tgactcaaat gcttccaagc tttatctcat
    15241 acgaagcctt cttgtctttc ttcggtcttg gattaccgat tacagtgcca agtttgggtc
    15301 gtttgatttc ggattattca caaaacgtaa caaccaatgc ttacttgttc tggattccat
    15361 tgacaaccct tgtcttggta tccttgtccc ttttcgtagt tggtcaaaac ttagcggatg
    15421 ctagtgatcc acgtacacat agataggagt agaaatgaca aaagaaaaaa atgtaatttt
    15481 gactgctcgc gatattgtcg tggaatttga cgttcgtgac aaagtattga cagccattcg
    15541 cggcgtttcc cttgaactag tcgaaggaga agtattagcc ttggtaggtg agtcaggatc
    15601 aggtaaatct gttttgacaa agaccttcac aggtatgctc gaagaaaatg gtcgcattgc
    15661 ccaaggtagt attgactacc gtggtcagga cttgacagct ttatcttctc acaaggattg
    15721 ggaacaaatt cgtggtgcta agattgcgac tatcttccag gacccaatga ctagtttgga
    15781 ccccattaaa acaattggta gtcagattac agaagttatt gtaaaacacc aaggaaaaac
    15841 agctaaagaa gcgaaagaat tggccattga ctacatgaat aaggttggca ttccagacgc
    15901 agatagacgt tttaatgaat acccattcca atattctgga ggaatgcgtc aacgtatcgt
    15961 tattgcgatt gcccttgcct gccgacctga tgtcttgatc tgtgatgagc caacaactgc
    16021 cttggatgta actattcaag ctcagattat tgatttgcta aaatctttac aaaacgagta
    16081 tcatttcaca acaatcttta ttacccacga ccttggtgtg gtggcaagta ttgcggataa
    16141 ggtagcggtt atgtatgcag gagaaatcgt tgagtatgga acggttgagg aagtcttcta
    16201 tgaccctcgc catccatata catggagtct cttgtctagc ttgcctcagc ttgctgatga
    16261 taaaggggat ctttactcaa tcccaggaac acctccgtca ctttatactg acctgaaagg
    16321 ggatgctttt gctttgcgtt ctgactacgc aatgcagatt gacttcgaac aaaaagctcc
    16381 tcaattctca gtatcaaaga cacattgggc taaaacttgg cttcttcatg aggatgctcc
    16441 aaaagtagaa aaaccagctg tgattgcaaa tctccatgat aagatccgtg aaaaaatggg
    16501 atttgcccat ctggctgact aggaggaagg aaatgtctga aaaattagta gaaatcaaag
    16561 atttagaaat ttccttcggt gaaggaagta agaagtttgt cgcggttaaa aatgctaact
    16621 tctttatcaa caagggagaa actttctcgc ttgtaggtga gtccggtagt gggaaaacaa
    16681 ctattggtcg tgctatcatc ggtctaaatg atacaagtaa tggagatatc atttttgatg
    16741 gtcaaaagat taatggtaag aaatcgcgtg aacaagctgc ggaattgatt cgtcgaatcc
    16801 agatgatttt ccaagaccct gccgcaagtt tgaatgaacg tgcgactgtt gattatatta
    16861 tttctgaagg tctttacaat caccgtttat ttaaggatga agaagaacgt aaagagaaag
    16921 ttcaaaatat tatccgtgaa gtaggtcttc ttgctgagca cttgactcgt taccctcatg
    16981 aattctcagg cggtcaacgt caacgtatcg gtattgcccg tgccttggtc atgcaaccag
    17041 actttgttat tgcagatgag ccaatttcag ccttggacgt ttctgtacgt gcccaagtct
    17101 tgaacttgct caaaaaattc caaaaagagc tcggcctgac ctatctcttc atcgcccatg
    17161 acttgtcggt tgttcgcttt atttcagatc gtatcgcagt tatttacaag ggtgttattg
    17221 tagaggttgc agaaacagaa gaattgttta acaatccaat tcacccatat actcaagcct
    17281 tgctttcagc ggtaccaatc ccagatccaa tcttggaacg taagaaggtc ttgaaggttt
    17341 acgacccaag tcaacacgac tatgagactg ataagccatc tatggtagaa atccgtccag
    17401 gtcactatgt ttgggcgaac caagccgaat tggcacgtta tcaaaaagga ctaaactagt
    17461 aatggtttta taatttccat gtcaattttt atggaaatta taaaactttt tttgttggac
    17521 tgatttgaaa aactttgaaa gcttcctgaa agaatttttg aaaaatttta aaaattatga
    17581 aatgttattg acaggtgatg aaaaaggtgg taggacagga agcatcaaaa aagaaagcag
    17641 aaaaagaaat gaataagaga caagccgtaa ctatgaatca aaacttttac tttagctact
    17701 ttagatagtg tttgtagaca tgtcaccatg gatgcaaaca gttcaagcaa tttgtttgta
    17761 tctatgtggc taagattttt gattgtggtc catatagatg aatatctaaa tggactagct
    17821 aagttgtaac ttgttaggtt atttcaagta tccgtttaga tagtatctag gcggtttttt
    17881 gttttatctt ttctgagaag gagtttcatt atgaaagttg ttcgaaaatc actagccccc
    17941 tcttggtagt ggggctcctc ttgacttctc tgatcagttt gcatcagttg aaggcagata
    18001 agaaaaagat gtctttcgta tcggtatttc gcaatttatt acccaccagt ccttatacgc
    18061 tactagagaa ggatttgtgg atgagctggc caaacaaggc tatgttgagg gggaaaatat
    18121 tgagattgat ttgcaaaatg cacagggaga acaaagaaat ctaaaaacga tttcccagca
    18181 actagcagaa tccagcgatg tcgttctagc catcgcaagg ccttctgctc agagcttggc
    18241 caatacgacg caaacgacac cagttatctt ttcagctgta acagacccag tcagtgccaa
    18301 gttggttgag tcaagagaac atcctggggg caatgtaact gggacaagcg atcagtcatc
    18361 agatgccatt tcaacccaaa tcaatttgat aaagaaagtg ttgccaaagg ctaaaacgat
    18421 tggaatcctc tatactcaga gcgagcccaa ttcggttgtt caaaaggatg aagctaagcg
    18481 acttctgaaa gaaaaaggct ttaccgttgt tgaaaaaaca atcttggaca gtaataacgt
    18541 caaggcggca gcagagagtt tgatggcaga ggtggatatg gtttttgtcc caactgataa
    18601 tatcatttcg ttaaccatgg aaacagttaa gcaggtttct attaaacaca aggttccagt
    18661 attcggtggt tcaacagaaa tgattgctgt tggtggcttg tataactacg gaaccaatta
    18721 tgaagaattg gtacgtcaga cagctcgcat gctgattcgt gttttaaaag gtgagaaacc
    18781 agaaaatata gcagttgagt tgcctgaaaa actggaattg cataccaatc aagaaatggc
    18841 agatgcattg ggaattgata ttagtaagtt agaaggcaag gaataaggag gattaagatg
    18901 aattttgtgt tatctagttt atcagaaggt ttgttgtggt cgattgtggc gattggggtc
    18961 tacttgactt tccgtatttt ggatattgcg gatatgactg ctgagggagc ctttcctctg
    19021 ggggcagcag tcgttgtatc acagatacag gcaggggcaa atccttggct tgcgacttta
    19081 cttgctttgc tgacaggtat ggtagcaggt cttgtatcag gaatgctcca caccaagatg
    19141 aaaatcccag ctctcttgac agggattgtg accttgacag ggctctattc aatcaatatt
    19201 aaaatcatgg gcagtgtacc caatctttcc ttgggagatt ctgcaaccgt ctttaaacag
    19261 ttggcgagct tgggtctgac aaatgaagga actgttttct cacttagttt ggcctgtttt
    19321 ttacttgttt gtttggtctt gacccttttg atgaaaacag agattggcct ggtcttgcgt
    19381 tcgactgggg ataatatccc gatgagtgag gccaatggag tcaatgtaga caccatgaag
    19441 attgttggtt acatgatttc aaacggtttg attgctctat gtggttcctt gtttgcccaa
    19501 aatgatggat tttcagatgt gacttctggg acaggaacca ttgttgttgg tttgagctcg
    19561 gtgattattg cggaagtctt gatacacgac ttgaccattg gaggccgctt attatccatc
    19621 ggaatcggtg ctattgttta ccgtttgatt attttaaata tctatgaaat tccaaatcta
    19681 gatcaaaact tggtacgtct ctttaatgca atcttgctcg ctttggtttt atttgcacca
    19741 gaattgcaaa agagattaaa gattcgtggt ctgaaattga gaaatgaata ggaggagaaa
    19801 gttatgacaa gtttgttaac acttgaaaat attcacaaga catttgaagc agggacggtc
    19861 aatgagaacc atgtcctcaa aggattagat ttagaggttg aagagggaga ttttatctct
    19921 gtgattggtg gaaatggagc aggaaaatcc actttgatga atatcttggc tggcaatcta
    19981 tcggtggacg aaggggacct tctactggca gggaaatcca ttaaaaatct gagtgtcaga
    20041 aagagagcca aggatattgc ccgtgttttt caagatccta agatgggaac agcttctcgt
    20101 ttgacgattg aggagaatat ggctattgcc ttgagacgcg ggcagaagag agggcttggt
    20161 tggggcgtga aggagaagga tagaatccag ttccaagagg ccttgaagga gttgaatatt
    20221 ggtcttgaaa accgcttaaa agtagatact caatatctct caggaggaca aagacaggct
    20281 ttgactctag tcatgacaga tctgatgaaa ccaaaacttt tacttttgga tgaacatact
    20341 gcggcacttg acccgaaaac cagtcaaatg gtgatggact tgacgcaaaa gattgtggag
    20401 caccatcagt ggacgacttt gatgattacc catgatatga atcatgcgat tgagtacggc
    20461 aatcgtctca ttatgctcta tcaaggcaag atagtggtgg atgtcaaggg agaagagaaa
    20521 aagcatctga cagttgaaga ccttatgcat ctcttccaga aaaatagtgg ccaaagtctg
    20581 gtcagtgatg aattagtttt gggataaaga aaaaggctga gatctggtgt ctcagtcttt
    20641 tatttcttgc gttttgcaaa atctacaaat ctaaatttct ctaacttatg gcgactttcg
    20701 gtaaactgaa attggcgtcc gtcttggaga tagacttttg atttaatcga aacgacataa
    20761 gggtctttgc caatgtccat gagaatcttg tctcggtcgc ttgagtggtc aatggtgatt
    20821 tccttctgag cataatcaat aaggagtttg aggccatttt ctatataaga atagatagac
    20881 tgctcagcaa tttggcgagt gagatttgga attagttcca tatccagata gtccgtatcc
    20941 agaacggata ccagatcatc caccacacgc tggcggacca ccttccaaac catccgaaac
    21001 tctgggaaac cggttatcag tgaggatttt ttatcaataa taatcttgtc cagactgacc
    21061 acgttggttt tagagcgcag tccaagttct ttaactagtt cttggtagct ggttaggttg
    21121 gatacaggga aattgacggt ttcttctttg acgacttgag aaccttgctc tcttatcttt
    21181 ttgatcaatc cttcctcttg gaggagagac agggcctttc ggacggtatc acgactcact
    21241 tgatattgct ccataaggtc gtgctcgcta ggaaggaaat ctccgacagc gtaagtctcg
    21301 ttttgaatgg tttcttggat ttgcttaaat aattgttggt atttcttcat tttgcctgct
    21361 ttaataggtt tttagtctag tttaatgaac acctgtatct attttaccat acaagtgcta
    21421 gaaaatttac aaaaaaacac cctttttata ccaaacaagt tgcagacaag tttggtatcg
    21481 tttacaatat acttatcaaa tcaaacttgc tttgacaagt ataaggagaa tcaaatggga
    21541 aaatttgaac aagaagccaa agatctgctt caggcaattg gaggcaaaga aaatgtgact
    21601 gccgtaactc actgtgcgac acggatgcgc tttgttttag gagatgataa gaaggctaat
    21661 gttaaagcta tcgagtcaat tccagctgtt aaaggaactt ttacaaatgc aggtcaattt
    21721 caggtaatca ttggaaatga cgtgcccatc ttttataatg attttacagc cgtttcaggt
    21781 attgagggtg tttccaaaga agcagccaag tctgcagcta agagtaatca aaacgtggtc
    21841 caacgtgtta tgaccactct ggcggagatt tttactccga ttattccagc cttgatagtc
    21901 ggaggattga tcctcggttt ccgtaatgtc ttggaaggtg tccattggtc gatgttggat
    21961 ggcaagacca tcacagaatc ctctcagttt tgggcaggtg tcaatcactt cctctggttg
    22021 cctggtgaag ctatcttcca gttcttacca gtagggatta cttggtctgt ttctcgtaag
    22081 atgggaacca gccaaatttt gggaattgtt ctcggaatct gtttggtatc gcctcagttg
    22141 ctcaatgcct atgcggttgc ttcaacgcca gcagctgata tcgcggcaaa ctgggtttgg
    22201 aattttggct attttactgt taatcgtatc ggttaccaag cccaagttat cccagccttg
    22261 cttgcaggtt tgagtctgtc ttatcttgaa atcttctggc gcaagcatat cccagaagtc
    22321 atttctatga tttttgtacc tttcttgtca ttgattccag ccttgatttt ggctcatact
    22381 gttttgggac caatcggttg gacaattgga caaggacttt catcagttgt cttggcaggt
    22441 ttaactggtc cagttaaagg gctcttcggt gcaatttttg gtgccctcta cgctccattt
    22501 gtcatcacag gtctgcacca tatgaccaat gccattgata cacaattgat tgcggatgct
    22561 ggtggcactg ccctatggcc aatgattgct ctttctaata ttgctcaagg ctcagccgtg
    22621 tttgcctatt atttcatgca tcgccatgat gagcgtgagg ctcaggtttc acttcctgca
    22681 accatttcag cctatctcgg tgttacagaa ccagctcttt ttggggttaa cgtaaaatat
    22741 atttatccat ttgttgctgg gatgactggt tcagcccttg caggcatgtt atccgttact
    22801 tttaatgtaa ctgcggcttc tattggtatc ggtggtttgc caggtattct ctctattcaa
    22861 cctcaataca tgctgccatt tgcaggaact atgctagttg cgattgttgt tccaatgctc
    22921 ttgactttct tcttccgcaa ggctggtctc tttacaaaaa cagagggcga tacgaacttg
    22981 caggcagaat tcgttgctca agaagaagca gaatttgtga accatgaacc agtagaactt
    23041 acttcggtag aaattatcag cccactaact ggccaagtga aagaattgag tcaagcgacg
    23101 gatcctattt ttgcatcagg tgtcatgggg caaggtctgg tcattgaacc aagccaaggt
    23161 gagttgacct ctccagttaa tgggacagtg acggttcttt tccctaccaa gcatgccatc
    23221 ggcattgtct ctgacgaggg agttgaattg ctcatccaca tcggtatgga tacagtaggt
    23281 cttgatggca aaggttttga aagtcttgta gtccaaggag atcacgttac agttggtcag
    23341 caactgattc gttttgatat ggatgtcatt aaggctgcag gtctggtgac agaaactcct
    23401 gttatcatca ccaaccaaga tgcttataca gcgactattc ccggaactta tccgacaacg
    23461 attcaagctg gagcatctct catggtcgct acacgaatct aatactcttc gaaaatctct
    23521 tcaaaccacg tcagctctat ctgcaacctc aaaacagtgt tttgagcaac ctgtggctag
    23581 cttcctagtt tgctctttga ttttcattga gtataattga aatagcacaa atagggacgg
    23641 aagactgctt cctccctttt atcagaaaag gagaaacaga tgacacttga taaaggaaaa
    23701 gtagtctatc aaatctatcc aaaatcttac aaagacacca ctgaaaacgg tattggggat
    23761 ttccgtggga ttatcgaaaa aattccctat ttagccaaac taggcgtgga tatggtctgg
    23821 ctcaatccat tctatccaag ccctcaacgg gataatggtt acgatatttc agattatatg
    23881 gcagtggatc ctctttttgg tgatatggct gattttgagg aaatggtgtg tgtcggtaaa
    23941 gagcacaaga ttgactttat gctggacatg gtacttaatc attgttcgac agagcatgaa
    24001 tggtttcaga aagccctagc tggtgataag tattatcaag actttttctt catccaagac
    24061 caaccaacag attggcagtc taagtttggt ggttctgcat gggcgccttt cggggatact
    24121 gggaaatact atcttcacct atttgatgag acccaggctg accttaactg gcgcaattcc
    24181 aatgtccgta aggagctttt caaggttgtt aatttctggc gcgacaaggg tgtcaaaggt
    24241 ttccgatttg atgtgatcaa tttgatcggt aaggacgagg tttcagtgga ctgtcctgaa
    24301 aatgaaggga agccagctta tacagacaaa cccatcgttc ataactattt gcgtatgatg
    24361 aaccaagcca cttttggatc tgacgatagc tttatgacag ttggggaaat gtcttctacc
    24421 accatggaaa actgtgtcct ctattcgtcg cctgatcgtc aggaattatc catgaccttt
    24481 aattttcatc acctcaaggt ggactataag gatggacaaa agtggacctt ggctcccttt
    24541 gattttgaag agttgaagag tctttaccat agttggggta aggaaatgag tgataaagac
    24601 ggttggagtg ccctcttttg gaacaatcac gatcaaccac gtgccttgaa ccgttttgtc
    24661 gatattcaaa acttccgcaa ggaaggagcc accatgctag cagccagcat tcacctgtca
    24721 cgtgggacac cttatatcta catgggtgag gaaatcggga tgattgaccc agactatgat
    24781 tctatggctg attatgtgga tgtcgaatcg cttaatgcct atcagatgct cttagaagaa
    24841 ggaaagagtc agcaagaagc cttccagatt attcaagcta agtcgcgtga taattcacgt
    24901 attcccatgc agtgggatac ttctgaaaat gcaggatttt caacaggcac tccatggttg
    24961 aaagtcggta aatcctataa agatatcaat gtagaaaatg aaatcaaagg tctgattttc
    25021 accttctatc aagacttgat tcgactgcgt aaggaaatgc caatcatttc agaaggaaat
    25081 tacaaaccag cctttgaaga tagcaaacaa gtctacgctt ttgaacgcca gtttgaggat
    25141 caaaagttac tagtgctcaa taacttttat gccaaagaag tggaaatcga cttgccagca
    25201 gtctaccaaa atggacagat tttgatttca aactatgaag atgcagaagt gtctgagaaa
    25261 attctactca aaccctatca aacactggct atttatgtaa attaatactc ttcgaaaatc
    25321 tcttcaaacc gtgtcagttt tatctgtaac ctcaaagcgg tgctttgagc aacctgtggc
    25381 tagcttccta gtttgctctt tgattttcat tgagtataaa cttagcaagg agacctcttt
    25441 ttcagaaaag cagggttctt tttgctgtct attccatcct tgaaagtgta gaacaaactg
    25501 gtcagggtgg ctagtttgtg gtataatgaa agggattcaa aaagaaacag gagaaaaatg
    25561 atggatttac ttttagcaat tgtattgatt gtgctagctt ttctaggagg agctcttgga
    25621 ggaatgtact tggttcgtaa gcaaatcgaa aaagaatttg ctgacaaccc acgtttgaat
    25681 gctgaagcag ttcgtactct tttgagtgca aatggtcaaa aaccaagcga agctaaggta
    25741 caacaagttt accaccaaat catccgccaa caaaaggcag cccttgctaa caataaaaag
    25801 aaaaaataaa taaggaaaag tctgggatga aagttccaga cttctcacta tgttggttat
    25861 ggtttaggga tgagactttt tccttgcatt ctattgatat ttgattatga ttaagagaga
    25921 ggtagttgac attctatcaa gctgtcggtc agttcgttca gtacaaggaa tcataatgaa
    25981 cgatcaatca gaaaaaagac tagaaagaag actgtatgga taatcgacca attggttttt
    26041 tggattcggg tgtcgggggc ttgaccgttg tgcgcgagct catgcgccag cttccccatg
    26101 aagaaatcgt ctatattgga gattcggcgc gggcgcccta tggcccccgt cctgctgagc
    26161 aaattcgtga atatacttgg cagctggtca actttctctt gaccaaggat gtcaaaatga
    26221 ttgtcattgc ttgtaacact gcgactgcgg tcgtctggga agaaatcaag gctcaactag
    26281 atattcctgt cttgggtgta attttgccag gagcttcggc agccatcaag tccagtcaag
    26341 gtgggaaaat cggagtgatt ggaacgccca tgacggtaca atcagacata taccgtcaga
    26401 aaatccatga tctggatccc gacttacagg tggagagctt ggcctgtccc aagtttgctc
    26461 ccttggttga gtcaggtgcc ctgtcaacca gtgttaccaa gaaggtggtc tatgaaaccc
    26521 tgcgtccctt ggttggaaag gtggatagcc tgattttggg ctgtactcat tatccactcc
    26581 ttcgccctat tatccaaaat gtgatggggc caaaggttca gctcatcgat agtggggcag
    26641 agtgcgtacg ggatatctca gtcttactca attattttga aatcaatcgt ggtcgcgatg
    26701 ctggaccact ccatcaccgt ttttacacaa cagccagtag ccaaagtttt gcacaaattg
    26761 gtgaagaatg gctggaaaaa gagattcatg tggagcatgt agaattatga caaataaaat
    26821 ttatgaatat aaggatgacc aggactggta tgttgggtct tatagtattt ttggtggcgt
    26881 taacagtttg agcgactata agacagattt tcctctgttt gaattctcca aaatatttgg
    26941 agatgaagag tatggtttcc cgctttcagt tactgtttta cgctatggtt ctacctaccg
    27001 tttgttctcc tttgtggtag acatgcttaa tcaagaaatg ggacgaaact tggaagttat
    27061 tcaacgtcat ggggccctgc tcttggttga aaatgggcaa ctcttgtatg tagaattgcc
    27121 taaagaaggg gtcaatgttc atgatttctt tgagacaagc aaggtcagag aaaccttgtt
    27181 gattgcgact cgtaacgaag gtaaaaccaa ggaattccga gctatctttg ataagttagg
    27241 ctacgatgtg gaaaatctta atgactaccc tgacctgcct gaagtagcag aaacaggtat
    27301 gacctttgaa gaaaatgccc gccttaaggc agaaaccatt tctcaattaa cgggcaagat
    27361 ggttttggca gatgattctg gtctcaaagt cgatgtcctt ggtggcttac caggcgtctg
    27421 gtcagctcgt ttcgcaggtg tgggagcaac tgaccgtgaa aataatgcca aactcttgca
    27481 cgaattggcc atggtctttg aactcaagga ccgctcggct cagttccaca caaccctagt
    27541 cgtagccagc ccaaataagg aaagtttagt tgttgaagca gactggtcag gttatattaa
    27601 ctttgaacct aagggtgaaa atggctttgg ctatgatccc ctcttccttg taggagagac
    27661 aggtgagtca tcagctgaat taaccctaga agaaaaaaat agtcaatctc accgtgcctt
    27721 agccgttaag aaacttttgg aggtatttcc atcatggcaa agcaaaccat cattgtaatg
    27781 agcgattccc atggcgatag cttgattgtg gaagaagtcc gtgatcgcta tgtgggcaaa
    27841 gtcgatgccg tttttcataa cggcgattct gaactacgtc cggattctcc actttgggag
    27901 ggcatccgcg ttgttaaagg gaacatggac ttctacgccg gctacccaga acgtctggtg
    27961 actgagcttg gttcgaccaa gattatccaa actcatggtc acttgtttga catcaatttc
    28021 aactttcaaa agttggacta ctgggctcag gaggaagagg ccgctatctg cctctatggt
    28081 cacttgcatg tgccaagtgc ttggttggaa ggcaagatcc tctttctaaa tccaggttct
    28141 atcagtcaac cacgaggtac catcagagaa tgtctctatg ctcgtgtgga gattgatgat
    28201 agttacttca aagtggactt tttgacacga gatcacgagg tgtatccagg tttgtccaag
    28261 gagtttagcc gatgattgcc aaggagtttg agactttctt gttggggcag gaggaaactt
    28321 ttttgacccc tgctaaaaat ctagctgtgt tgattgatac ccacaatgcg gatcatgcga
    28381 ccctcttgct cagtcagatg acctataccc gtgttcccgt tgtgacagat gaaaaacagt
    28441 ttgttgggac gattggactc agagatatta tggcttatca gatggagcat gacttgagcc
    28501 aagaaatcat ggcggatacg gatatcgttc atatgacaaa aacggacgta gcggttgttt
    28561 cgcctgattt caccattacg gaggtcttgc acaagctagt agatgagtcc ttcttaccgg
    28621 tcgtggatgc agagggtatt ttccaaggga ttattacgcg caagtccatc ctcaaggccg
    28681 ttaatgccct cttgcatgac tttagtaagg aatatgagat tcgatgccaa tgagagacag
    28741 gatttcagcc tttttagagg aaaagcaggg cttgtctgtc aattccaagc agtcctataa
    28801 gtatgatttg gagcaatttt tagacatggt aggtgagcgg atttctgaga ccagtctcaa
    28861 gatttaccaa gcccagctag ccaatctaaa aatcagcgcc cagaagcgaa agatttcggc
    28921 ctgtaaccaa tttctatact ttctctatca aaaaggagag gtggacagct tttaccgctt
    28981 ggaattagcc aaacaagctg aaaagaagac ggaaaagcca gagattctat acctagactc
    29041 tttttggcag gaaagcgacc atccagaggg ccgcttgcta gcgctcttaa tcctagaaat
    29101 ggggctcttg cccagtgaga ttttagccat caaggttgcg gacatcaatc tggattttca
    29161 ggtgctgcga atcagcaagg cttcccaaca gaggattgtc accattccca cggccttgct
    29221 ttcagaattg gaacccttga tggggcagac ctatcttttt gaaagaggag agaaacccta
    29281 ttctcgtcag tgggcctttc gtcagttaga atcttttgtc aaggagaaag gttttccatc
    29341 cttatcagct caagtcttac gtgaacagtt tattctaaga caaatagaaa acaaggtcga
    29401 tttgtacgaa attgcaaaaa aattaggatt aaaaacagtc ctgaccttag aaaaatatag
    29461 ataatggata ttaaattaaa agattttgaa ggacccctgg acttgctctt gcatctggtt
    29521 tctaagtacc agatggatat ctacgatgtg cccattacgg aagtcatcga acagtatcta
    29581 gcctatgtct caaccctgca ggccatgcgt ctggaagtga cgggtgagta catggtcatg
    29641 gctagtcagc tcatgctgat taagagtcgt aaactccttc cgaaggtagc agaagtgaca
    29701 gacttggggg atgacctgga gcaggacctc ctctctcaaa tcgaagaata tcgcaagttc
    29761 aagctcttgg gtgagcactt ggaagccaag caccaagaac gggcccagta ttattccaaa
    29821 gcgccgacag agttgattta cgaagatgcg gagcttgtgc atgacaagac gaccattgac
    29881 ctctttttgg ctttttcaaa tatcctagcc aagaaaaaag aggagtttgc acaaaatcac
    29941 acgacgatct tgcgggatga gtataagatt gaggacatga tgattatcgt gaaagagtcc
    30001 ttgattggac gagatcaatt gcgcttgcag gatttgttca aggaagccca gaatgtccaa
    30061 gaggtcatca ccctcttttt ggcaacccta gagttaatca aaacccagga gctgatcctc
    30121 gtgcaagagg agagttttgg agatatctat ctcatggaaa agaaggaaga aagtcaagtg
    30181 cctcaaagct agacttgata gagaggaaag atgagtactt tagcaaaaat agaagcgctc
    30241 ttgtttgtag cgggtgaaga tgggattcgg gtccgccagt tagctgaact cctctctctg
    30301 ccaccgacag gcatccagca aagtttagga aaattagccc agaagtatga aaaggaccca
    30361 gattccagtt tggctttgat tgagacaagt ggtgcttata gattggtgac caagcctcaa
    30421 tttgcagaga ttttgaagga atactctaag gcgcctatca accagagctt gtctcgggct
    30481 gcccttgaga ccttgtccat tattgcctac aaacagccga ttacgcggat agaaattgat
    30541 gccatccgtg gagttaactc gagtggagcc ttggcaaagt tgcaggcttt tgacctgata
    30601 aaggaagacg ggaaaaagga agtattgggg cgccccaacc tctatgtgac tacggattat
    30661 ttcctagatt acatggggat aaaccattta gaagaattac cagtgattga tgagcttgag
    30721 attcaagccc aagaaagcca attatttggt gaaaggatag aagaagatga gaatcaataa
    30781 gtatattgcc cacgcaggtg tggccagtag gagaaaagca gaagagctga ttaagcaagg
    30841 cttggtgacg gttaacggcc aagtggtgcg tgaactagca accactatca agtcaggcga
    30901 caaggtcgaa gttgaaggtc aacctatcta caacgaagaa aaggtctact atctgcttaa
    30961 caaaccacgc ggtgtgattt ccagtgtgac agatgataag ggtcgcaaga cggttgtcga
    31021 cctcttgccc aatgtcaaag agcgtattta ccctgtgggt cgtttggact gggatacatc
    31081 aggtgtcttg attttgacca atgatgggga ctttacagac gagatgattc accctcgtaa
    31141 tgagattgac aaggtttatg tcgcgcgtgt taaaggtgtg gccaataagg acaatctccg
    31201 ccccttgacc cgtggtcttg agattgatgg taagaaaacc aagccagctg tttatgaaat
    31261 tctcaaagtg gacccagtca aaaatcgctc tgtggtgcag ttgaccatcc atgaagggcg
    31321 taaccatcag gttaaaaaga tgtttgaagc tgttggtctc caagtagata agttgtctcg
    31381 gactcgtttc ggacacctag acttgacagg actccgtcca ggagaatccc gtcgtcttaa
    31441 taaaaaagaa atcagccaac tacacaccat ggctgtaact aagaaataat gaaacgaatt
    31501 ttaatagcgc ctgtgcgctt ttaccaacgt tttatctcac cagtctttcc accctcttgt
    31561 cgctttgagc tgacttgttc caactacatg attcaggcta ttgaaaaaca tggttttaag
    31621 ggggtattga tgggcttggc tcggatttta cgttgtcatc cctggtcgaa aacaggtaag
    31681 gaccccgttc cagaccactt ttcccttaaa cgaaatcaag aaggggaatg aggtggggta
    31741 aatagatttc aaaatgataa aaacgcatcc tatcaggttt gagtaaactt gataggatgc
    31801 gttttagaat gtcaaaattt tatactcttc gaaaatctct tcaaaccgcg tcagctttca
    31861 tctgcaacct caaaacagtg ttttgagcaa cctgcggcta gtttcctagt ttgctctttg
    31921 attttcattg agtattaaat tgagtttgaa gtggcttatt tcaaagcttt ttgtatgtct
    31981 tcaatcatga gttttgttga ttcaagtccg cctccgctta gataccagag gtctggtgtt
    32041 agttggataa tcttaccatt tttagcagca ggtgtttcag cgataagggc attttctagg
    32101 acaccgtcgt tgctagagtt gtccccaccg atggcaaggg tacggttgat gacaaagagg
    32161 atgtcagggt tgatttcttt gacactttca aagctgactt cttgtccgtg gcgtgagtct
    32221 tcaaattttg tatcagttgg tttgaatttc aaggtttggt acaagaaaga gaaacgagat
    32281 ttggcaccaa aggctgccat ttttccttca ttaaggagga tcgcaagggc ttttttgtca
    32341 gagctttcat ttttagtagc gacttcttgg atgctcttgt ctagcttggc caattcttcc
    32401 ttggctttct gtgtaccagt ttcgccgaag gcacttgcta aggattcgat attagccttg
    32461 gtagaagtcc agtagtcgtc cttgcttgct tggaagagaa cggttggggc gatttctttg
    32521 aatttgtcta cgaatttttg tgtacgtggt gaagcgataa tcaaatcagg ctcaagggcg
    32581 gcgatagctt ctaaatcagg ttctttcata gaaccaacat ttttgacagt tcccactagg
    32641 tcttttagat aagtcggaac agtttttgta ggcattccga cgatattttt ttcaaatcct
    32701 aaagcgcgaa tagtatccgc agcgccgagg tcaaaggtca caatcttttc aggaactttg
    32761 gaaagtttga cctcggccag tgaactttta atggttacct ctgttggagc agagctactg
    32821 gtctctgtct gactagtgct tgagtttgta ctacatgcac caagtaggag caagaagctg
    32881 gccactaggg cagtgaaata aagtttaagg gatgttttca taatttctcc tctttaaaat
    32941 gtgataacga tttagggagt ctcttgatct tattgactaa gagactgaag gttctctaac
    33001 ttgagctttt atgttactag ctatagatac agatcttttt gtcattgata tcagctagcg
    33061 tgatgggaat ctcataaagt tgactgagca ggtcagcctg catgatttga tcggttcttc
    33121 ccttgctaaa gacctggccg tccttgaagg cgacaatttc atctgcatac tgactggcta
    33181 tgttgatatc gtggaggacg atgataatgg tcttgccgag ttcctccacc agtcgtcgaa
    33241 gaatctgcat catgctgacg ctttgcttga tatcgagatt gttgagtggt tcgtccagca
    33301 agataaagtc cgtatcctgg gccagtacca tagcgataaa gacgcgctgg agttgccccc
    33361 ctgacaggct attgatgtag cggtctttta agttggtcag ttccaaatag ttcagagttt
    33421 ctcggatttt ttcccagtct tctgatctaa gtcgacctcg gctgtaggga aaacgtccaa
    33481 aactgaccag ttcttcaaca gtcaatttgg cttggtaatt gattttctgt tttaggatgg
    33541 ttagttcttg ggccagttct tgcgaattcc agctctcgat ttcacgtcct ttgatactga
    33601 gaactccctg atctttcttg gttagtctgc tcatgatgga gaggagagtc gattttccag
    33661 caccatttgg accaataaag gctgtcagtt tttgaggact gacttcaagc gaaatgcctt
    33721 gcaaaatatc ctgtttttga atggatttgt caatgttttc cagtttcact gacgagccct
    33781 cctatatagt aagataaaga ataagaagcc acccacactc tcaatgatca tactgatacg
    33841 aatttccagt gcaaagactc gttcaatcaa ggcttgcccc aaggttaagc taataaatcc
    33901 aaccagaatg gccactataa agagtaactt gtgctgatag tctttgacaa tcaggtaggt
    33961 gaggttggcc agcataaagc cgaagaaggc cataggtcct accaaggcag tggccgttga
    34021 ggtcaaaagc acgattcccc agaggagctc tttctgttct ttttcaacat cgagtcccaa
    34081 tatctgagcc gtttctcttt gcaggtgcaa gacatctaga acgactgctt ttcgaaagaa
    34141 aaagattgtc aaagcgagga tgatcagaga accgatggct aggatggaag tgttgagatg
    34201 ttgaaaggag gcaaaaagac tattttgcag tttatcgtat tcgtttggat ccattaggac
    34261 ttgaaggaag gtgctgatat ttcgaaagag acttctgagc gctagacaga tcagcaggat
    34321 gaagaccagg tcttgcttca tcagtgtctt caagtaacct tgtaaggcga gaaagaagag
    34381 ggactggaca agaagtaaga ctaggaattc taagataggg gatttgccaa gttgaagaaa
    34441 cttgctttca aaaaccagta gtagggtttg tagtaggacg tagaaggatt caattcccaa
    34501 aatactaggc gtcaggaagc gattttccgt cagggtttga aaactaatgg tcgaaatccc
    34561 agtcgcgatg gctaccaaga gataaacgat gatcttttgg gaacgcaact tccaagcaaa
    34621 ggctgacaag tgagtgatgg gccaaaagta gagaagacaa gctccgatgg caagaataat
    34681 gagaatccag aagagcttgg tatgtttgct tttagtctgc atcttttcgt ccccctctcc
    34741 agagaagtag gataaagacg agactaccga tgattcctag caagagactg acagacaact
    34801 catagggcct aatcagaact cgggatagga tatcgcaagc cagaactaga ttggcaccaa
    34861 ccagtgcgac catgagtttg gtttgactta gattatctcc atagcgcttg cgaacaagat
    34921 tgggaacgat aactccgaga aatggtaggc cacccacggt aatcatggtg acgcttgtcg
    34981 ttagcgccac cagaaagagg gccagttttt caagtaggga gtaggaaatc cccaaactct
    35041 cgctggtttc tttccctaga ttcatgatgg tgaaggtttg ggataatttc caaacggtta
    35101 tcaggatgat gaggcctaag aagagccact catactgatg ggtctgaatc atggagaagg
    35161 agccctgggt ccaggcagtc atactctgaa ccagattgaa acgataggcg ataacttctg
    35221 tgactgagcc gataatcccg ctatagatga tcccaatcag aggcaacatc cacctttcct
    35281 ttacagtaaa aatggtcata aaggctagga agaagagggt gaatacgatg gatgaaacaa
    35341 aagcgaagag catcttttgg gtcagactag ccgatggaaa gacaaaaagg ctcagcacca
    35401 ttcccagttt ggcggcttca gtcgttccaa ctgtactcgg tgcagcaaac tgattttggg
    35461 taatagtctg catgagaagg cctgccatac tcatactaga ggcagtcagg agaatactga
    35521 tagttcttgg gagacgggac tcttgaaaga ggagccaggt ctgctggtcg aaatcaaata
    35581 gctttcccca tgaaaaatca ctggtcccaa tgctaataga gagaaagact aggagtagaa
    35641 gtaagccaat taaataatga gaaagtttca taccccgtcc tttcatgtag atttggtatc
    35701 gaaagatatc tgcggatata aatgtaacat tatttttcta atctgtcaat aaaatttctg
    35761 acaatttaat aaatacaaca aggagagagc aacaagactt tctcctttgt tatcctattc
    35821 taaaatgttt ttaccttaat ctgataaaat aatatcttcg agggagtagc tagccgtcca
    35881 atcaagatat tgtttagctt ttgaagcatc tgctaggaca ctggctgggt cactagcacg
    35941 tcgagcaaca atctcgtgtg ggatttttta atttagtaat tcttcagcag ttttaaagat
    36001 ttctttgata gtatagcctt ttttagttcc taagttaaag atttgagaag aactgtcttc
    36061 ttgaaatagg tagttcattc ctttaacatg agcctatgca aggtccaaga cataaatgta
    36121 atctcgaata catgaaccgt cacgtgtatc gtagtcatct ccaaatattt ttaagctatc
    36181 attttgtccc aatgcggtct tgttgatatt tggaatgatg tgagttggat ttttcacacg
    36241 cagaccgttt gaagcatcca tttcagcccc agcaacatta aagtaacgga aaataacata
    36301 tttccagtcg tagcgattgg ccatccagta aatcattcgt tcgcccatca gttttgtctc
    36361 tgcataaggg ttgacagggt cgagcagggt atcttcagtc accggcttgt caatacagtt
    36421 atttccatag agagaagcag tcgaagagaa catgattttt tgaatgccaa cttcagataa
    36481 gactttgaga acttggttca taccagcaac gttggcagtg aagtatttac ttggattttc
    36541 aatactttcg ctcacaacaa tttcacctgc acaatggagg acagcatcaa tctgattttc
    36601 ttctaagtaa gcctttaagg cgctagcatc ataaacatcc agttctttaa agctagcacg
    36661 actatccaca gccgctcgat ttcctgtgga gagattatcg aggacatgca ccttatagcc
    36721 agcatttaaa agagctttaa cggtatggga gccaatgtag ctagtcccac ctgtaacaag
    36781 aattgttttg gtcatgattt tttccttttc tagttttgta ctatagtaaa ttgaaataag
    36841 atatgaacaa ctctattagg aaagtcaaat taatttctag aaatgtttca gcagctacag
    36901 cgtactattc caaactcaac caactatata tttaaagaaa gaagaaaggg aagtcaacta
    36961 ttttctgtaa atttcatgaa aaatccaaac aactttgatg gtgtattgtt tttttgtttg
    37021 gaaataaata ttcgagttaa ttttggaatg tttactagat tttgcactac atgcaagaga
    37081 atactatcct tttctaagat agagcaggac taagatacta acttagaaat ccaccttccc
    37141 actatccttc tcctataaaa agtggtaaaa tggatgaaag aaagaaggaa ctgaaatgac
    37201 aacattattt tcaaaaatta aagaagtaac agaacttgct gcagtctcag gtcatgaagc
    37261 gcctgtccgt gcttatcttc gtgaaaagtt gacaccgcat gtggatgaag tggtgacaga
    37321 tggcttgggt ggtatttttg gtatcaaaca ttcagaagct gtggatgcac cgcgcgtctt
    37381 ggtcgcttct catatggacg aagttggttt tatggtcagc gaaatcaagc cagatggtac
    37441 cttccgtgtc gtagaaatcg gtggctggaa ccccatggtg gttagcagcc aacgtttcaa
    37501 actcttgact cgtgatggtc atgaaattcc tgtgatttca ggttctgttc ctccgcattt
    37561 gactcgtgga aaggggggac caaccatgcc agccattgcc gatatcgttt ttgatggtgg
    37621 ttttgcggac aaggctgagg cagaaagttt tggcatccgt cctggtgata ccatcgtacc
    37681 agatagttct gcaattttga cagccaatga aaaaaatatc atctcaaaag cttgggataa
    37741 ccgctacggt gtcctcatgg taagcgagct agctgaagct ttatcaggtc aaaaactcgg
    37801 caatgaactc tatctgggtt ctaacgtcca agaagaagtt ggtctgcgtg gcgctcatac
    37861 ctctacaacc aagtttgacc cagaagtctt cctcgcagtt gattgctcac cagcaggtga
    37921 tgtctacggt ggtcaaggca agattggaga tggaaccttg attcgtttct atgatccagg
    37981 tcacttgctt ctcccaggga tgaaggattt ccttttgaca acggctgaag aagctggtat
    38041 caagtaccaa tactactgtg gtaaaggcgg aacagatgca ggtgcagctc atctgaaaaa
    38101 tggtggtgtc ccatcaacaa ctatcggtgt ctgcgctcgt tatatccatt ctcaccaaac
    38161 cctctatgca atggatgact tcctagaagc gcaagctttc ttacaagcct tggtgaagaa
    38221 attggatcgt tcaacggttg atttgattaa acattattaa accataaggg aggtggtagg
    38281 gatggtagaa ccaaacctag aaagccttat aaaagatctt tacaatcatg ctcgacatga
    38341 tttgagtgaa gatttagttg ctgctctcct agagactact aaaaaactgc ctactacaaa
    38401 tgagcaattg caggcagttc gtctctcaag cctggtcaat cgtgaattgc tcctaaatcc
    38461 caaacatcca gcacctgagt tgctcaactt ggctcgcttt gtcaaaagag aagaagccaa
    38521 gtacagagga actgcgactt ctgcgcttat gtatgaggaa ctttttaaaa tgctttgatt
    38581 ccattgtgga tcaaagcatt ttttataaga cctatctcgt gagcgttcaa atagtagtaa
    38641 caaggaatca tgagaagtca gccgagacca tagtagtgaa gaaacttctg taatggaagt
    38701 ggagtgaagt gaggaatcat caaacggtag gggataggct gtttgaaggg tgctacgaaa
    38761 aagatagggt aaggtgaaat tatagcggaa tgaaataaga tatgaacaaa ttgattaggg
    38821 aaatcaaatg aatttataga aatcttttag cagtcatggc gtcctattct agtttcaaaa
    38881 tgctataaat acgaacaaat tgattgggaa attcaaatcg ctttctatca atgttttaga
    38941 agatacagtg ccctagtcca acttcaatat actataaaag aacagatgga agcaattatg
    39001 atttacttct atctttaaaa taaatgatct gataggaaaa atgtgtcccc tcataaaaaa
    39061 taggatagta cggatggaag gcttttgtag actggaggac gaaggaattt agaaactctc
    39121 gttaaaagtt agttgagccg gagtatggtg gtgtgaataa ttatcaaaaa ttatttccaa
    39181 atcgaaatag ttgacagtta gaacgatgag tgttacaatt attttattca tttcgatatc
    39241 gaaataaatt ggaggtgtta tgaaagatag tcatttgcta gcccatcata ttcgtttgtt
    39301 gaatgggcgg atttttcaaa agttactgag tcaagatcct gaggctcttt ataggggtga
    39361 acagggcaag attttagcgg ttttatggaa tagtgaaact ggctgcgcaa ctgcgacaga
    39421 tatcgcgctt gcgactgggc ttgcgaataa tacgctgacg actatgataa aaaagctaga
    39481 ggaacaaaag cttgtaattg ttagtccgtg tggaaaagac aagcgtaaga agtatttagt
    39541 tttaacggag ttaggcaagt tccagaaaga agtggggcat cgtgtcagtc agaaattgga
    39601 tactatcttt tacaaaggat tttcagagga agaaattcac caatttgaag gttttcaaga
    39661 aagaattttg gcaaatctga aagagaagga aaatgaggtt tagaatatgt cacaacaagt
    39721 gaaaaatgct cacaacctat acatccatgc cattcaagac ggacgagttg ctgaggctca
    39781 agcccagtct gtaggggata cctatatcca acattcgaca ggtgttcctg acgggaaaga
    39841 aggatttgca gccttctttg cagatttctt tgagcgtcat ccagagcgtc agattaagat
    39901 tgtccgcacc attgaggatg gcaatctggt ctttgttcat gttcatcaat atctgaatgg
    39961 tggagaagct caatgggtga cgacggatac tttccgtgcg gatgagaatg gtcgtattgt
    40021 tgagcattgg gatgtcattg actactatcg aacacctgaa aatgaccaac tagatcaaat
    40081 ttttggagat tttgaaatca aggatttgga caagaaagca gaaaataaaa agctggttcg
    40141 ccgtttcttg acagaaactt tccaaaatgg ggagctagag cagtggagtg attatgtggc
    40201 agacgatttg attcagcata atcatgagat tggacaagga agtgctgctt ataaaaacta
    40261 tgtggctgaa tatagtgtca cttttgactt tgttttccaa ctcttaggac aaggaaacta
    40321 tgtggttagc tatggtcaga ctcagattga tggcgttgct tatgcccagt acgatatctt
    40381 ccgtttagag aacgggaaaa ttgtggagca ttgggataat aaggaagtca tacctaaggt
    40441 agaagacttg actaatcgag ggaagtttta aattgaggac aaagaatgat tgaatacaaa
    40501 aatgtagcac tgcgctacac agaaaaagat gtcttgagag atgtcaattt acagattgag
    40561 aatggggagt tcatggtttt agttgggcct tctgggtcag gtaagacgac catgattaag
    40621 atgattaacc gtctcttgga accaactgat ggaaatattt atatggatgg caagcgcatc
    40681 aaagactatg atgagcgtga acttcgtctt actactggat atgttttaca ggctattgct
    40741 ctctttccca atctaacagt tgaggaaaat attgctctca ttcctgaaat gaagggttgg
    40801 actaaggaag aaattgctca aaaaacagaa gagcttttgg caaaagttgg attaccagta
    40861 gctgagtatg ggcatcgact tccaagtgag ctatctggtg gagaacagca acgggtcggt
    40921 attgtccgag ctatgattgg tcagcccaag attttcctca tggatgaacc cttttcggcc
    40981 ttggatgcta tttcgagaaa acagttgcag gttctgacaa aagaattgca taaagagttt
    41041 gggatgacaa cgatttttgt aacccatgat acggatgaag ccttgaagtt ggcggaccgt
    41101 attgctgtct tgcaggatgg agaaattcgc caggtagcga atcccgagac aattttaaaa
    41161 gtgcctgcaa cagactttgt agcagacttg tttggaggta gtgttcatga ctaatttaat
    41221 tgcaactttt caggatcgtt ttagtgattg gttgacagcc ctatctcaac atttgcagtt
    41281 gtcacttttg accttgttac tagctatttt gcttgcgatt cccttggctg tttatcttcg
    41341 ctatcatgag aagttggcgg attgggtctt acagattgca gggattttcc agaccatccc
    41401 gtctctggcc ttgttgggac tctttattcc cttgatggga attgggactt taccggcatt
    41461 gacagctcta gtgatttatg cgatttttcc gattttacaa aatactatta ctgggctgaa
    41521 gggaattgat ccgagtcttc aagaggctgg gattgccttt gggatgacca gatgggagcg
    41581 tctcaagaag tttgaaattc cacttgccat gcctgttatc atgtctggaa ttcggacggc
    41641 agcggtcttg attatcggta cggctacctt ggcgaccttg attggggcag ggggactggg
    41701 ttcctttatc ctgttgggaa ttgaccgtaa taatgccagt ttgattttga ttggggccct
    41761 ttcttctgca gtgctagcta ttgcctttaa cttcctacta aaagtaatgg aaaaagcgaa
    41821 attgcggacg attttctctg gttttgcctt ggtggcttta ttactgggtc tgtcttatag
    41881 tccagccctt ttggctcaaa aagagaagga aaacttgatt attgctggga aaataggccc
    41941 agaaccagaa attttggcta atatgtataa attgctgatt gaagaaaata ccagcatgac
    42001 tgcgactgtt aaaccgaatt ttgggacgac aagtttcctt tatgaagctc tgaaaaaagg
    42061 tgatattgac atttatcctg aatttaccgg tacggtgact gaaagtttgc ttcaaccatc
    42121 acccaaggtg agtcatgagc cagagcaggt ttatcaagtg gcgcgtgatg gcattgccaa
    42181 gcaggatcat ctagcctatc tcaaacctat gtcttatcaa aatacctatg ctgtagctgt
    42241 tccgaaaaag attgctcaag aatatggctt gaagaccatt tcggatttga aaaaagtgga
    42301 agggcagctg aaggcaggct ttacgcttga gtttaacgac cgtgaagatg gaaataaggg
    42361 cttgcaatca atgtatggtc tcaatctcaa tgtggcgact atgcagccag ctcttcgtta
    42421 tcaggcaatt cagtcagggg atattcaaat cacggatgcc tattcgactg atgcggaatt
    42481 ggagcgttat gatttacagg tcttggaaga tgacaagcaa ctcttcccac cttatcaagg
    42541 ggctccactc atgaaagaag ctcttctcaa gaaacaccca gagttggaaa gagttcttaa
    42601 tacattggct ggtaagatta cagaaagcca gatgagccag ctcaactacc aagtcggtgt
    42661 tgaaggcaag tcagcaaagc aagtagccaa ggagtttctc caagaacaag gtttgttgaa
    42721 gaaatgatgg gacagagact ttaaacatac tgaactcaac ttccttaaac gactagataa
    42781 aagaatgctc agacaatttc gtctgggctt ttttgatgtg agaatgactg aatgtcactt
    42841 aaaatggaaa gaaagagaaa attttcatga ttttctaaaa ttttactgta aagacagttg
    42901 actattcata aaataggtgt ataatatttt gtgaactact taacaaataa gaggtttcag
    42961 ctcatgtcga ctaaggttag aaatcttgtg tataggcagt tcattatcta atagagcgtt
    43021 gcgtccgaaa gtctatccag acacggctct ttaaaaacaa aaggagaaat gatgcatact
    43081 tatttgcaaa agaaaattga aaatatcaaa acaaccctag gtgaaatgtc aggtggttac
    43141 cgtcgtatgg ttgcggctat ggctgattta ggattttcag gaactatgaa ggctatctgg
    43201 gatgacctct ttgcccatcg tagttttgcc cagtggattt atttgctggt tttaggaagt
    43261 tttcctctct ggctggagtt ggtttacgaa catcgtattg ttgactggat tgggatgatt
    43321 tgtagcttga cagggattat ctgtgtaatc tttgtatcgg aaggtcgagc aagtaattat
    43381 ctttttggct tgattaactc tgttatttac cttattttgg ccctacagaa aggcttttat
    43441 ggtgaggtgc tgacgacact ttacttcaca gtcatgcagc caattggact tctagtttgg
    43501 atttatcagg cacagtttaa gaaggaaaag caggagtttg tcgcgcgtaa actggacggc
    43561 aagggctgga caaagtatct ttccattagt gtgctttggt ggttggcctt tggcttcatt
    43621 tatcagtcta ttggtgccaa tcgtccctat cgtgattcaa tcacagatgc aaccaatggg
    43681 gtagggcaaa tcctcatgac agctgtttac cgtgaacagt ggatattctg ggcggctacc
    43741 aatgtttttt caatctatct ctggtgggga gaaagcctgc aaattcaagg gaaatatcta
    43801 atttatctca ttaacagtct agttggttgg tatcaatgga gcaaggcagc taagcagaat
    43861 actgatttac ttaactagga aaagatgttt gaaagtgctg ttttgagatt tcgattaaaa
    43921 cagatatagt tgataatcaa ggatttatag tatgaaaaag aggatcggcg ggtcctcttt
    43981 tgttgttgaa aagataaaaa actcagtaac ctagaaataa gacaactgag ctttactcta
    44041 tattcaattt ttaggaatga gaaggtctag ataaaattgg acaacttcct ggcctgtgaa
    44101 atcttgacct tttttgagcc accaggtcaa tgtctcgata aagttggaca cgaccaagtg
    44161 ttggaggtaa gaagtaggca tattagggtg ggcttctttt aaattatcag ctagcacgga
    44221 atagacatgg tgttctagct ctttatggag ttgacggagg aagtagtcat ttttggaaaa
    44281 tagcagactg gtgatatggt cttggttttt atgaaaatgg agaaagaggt gggcgaggta
    44341 gtcctcggtt gaaatggctt gctctctttc aaaaagatga tggaagaggt agcggcagag
    44401 ctggtccaga agaagctcct tactttcata gtgacagtaa aaggtggatc ttcccacatc
    44461 tgcgagatca atgatatcct gaacagtagt ggcctcgtag cccttagcat tcaaaagttg
    44521 tataaaagct tgatagatgg cttttttggt tttgctgata cggcggtcaa tgttagtcat
    44581 atggacactt aaggcaaatt gttcagaact gaataaagct gacgttttgc ttctatcctt
    44641 tctttgagtt ttagtggata atgataatga acaaggtgtt cataaatcta ttataacaaa
    44701 ggaatgagaa atatgaaggc aaaatatgct gtttgggtgg cttttttctt aaatttgact
    44761 tatgccattg ttgagtttat tgcaggtgga gtatttggtt ctagcgctgt tcttgctgac
    44821 tctgtgcatg acttgggaga tgcgattgca attggaatat cagcttttct agaaacaatc
    44881 tccaatcgtg aagaagacaa tcagtacacc ttgggctata agcggtttag cctgctagga
    44941 gccttggtaa cagctgtgat tctcgtaacg ggctctgttc tagtcatttt ggaaaatgtc
    45001 acgaagattt tgcatccgca accagtcaat gatgagggga ttctctggtt aggaattatt
    45061 gcgattacta tcaatctgtt agcgagtctg gtggttggta agggaaagac aaagaatgag
    45121 tctattctga gtttgcattt tctggaagat acgctagggt gggtagctgt tatcctgatg
    45181 gcgattgttc ttcgatttac ggactggtat atcctagatc ctcttttgtc ccttgtcatt
    45241 tctttcttta ttctttcaaa agcccttcca cgtttttggt ctacactcaa gattttcttg
    45301 gatgctgtgc cagaaggtct tgatatcaag caagtaaaga gtggcctgga gcgattggac
    45361 aatgtggcca gccttaatca gcttaatctc tggactatgg atgctttgga aaaaaatgcc
    45421 attgtccatg tttgtctaaa agaaatggaa catatggaaa cttgtaaaga gtctattcga
    45481 attttcctaa aagattgtgg ttttcaaaat attaccattg aaattgatgc tgacctagaa
    45541 actcaccaaa cccataagcg aaaggtgtgt gacttggaac ggagttatga gcatcaacat
    45601 tagaaaaaag tgaaaaatac ttgggtacta tcttatttgg aatagagtaa tttctttatt
    45661 atttaaatat ttcaaaaatt ggtaagagaa gagcattgta taaactccgg atatatgatt
    45721 gttaatgata aaaatttttc gattagatac aaaatgcttg acttggagtc aactcaaagt
    45781 tatataataa gataagtgag ttagaatagc gtgaattcag tgaatgaaat gagaggaggt
    45841 tagcgtgtga atattaaatc tgccagtgat ttgttgggaa tttcagcgga tacgattcgg
    45901 tattatgaac gggttggtct tgtgccaccg attactcgta ctgctactgg gattcgtgat
    45961 tttcaagatc aggatatcga agcgctggaa tttattaagt gttttcgttc ggcgggtgtc
    46021 tctgtagata gtttagttga ctatatgtcg ctctaccaaa agggagatga aacgagagag
    46081 gagaggcttg gtattttaga agaggaaaag caaaaattag aggagcgctt gtctcagcta
    46141 cagacagctt taaatcgttt aaatctcaaa attaaacttt ataaggaagg aaaattttaa
    46201 atgaaatcag cagtatatac aaaggcaggt caggttggac ttgctagcat tgaacgtccg
    46261 caaataatag aagcggatga tgtgattatt cgtgtggttc gtgcgtgcgt ttgtggttca
    46321 gatttatgga ggtaccgtaa tccagaaacg aaagctggac ataaaaatag tggacacgaa
    46381 gcgattggga ttgttgaaga agctggggaa gccattacga cggtgaaacc aggtgatttt
    46441 gtgattgtcc cttttacaca tggatgtggt gagtgtgatg cctgtcttgc tggatttgac
    46501 ggttcttgcg acaatcatat tggcaataat ttggggggtg attttcaggc agaatatatt
    46561 cgcttccact atgcaaactg ggcgctggtt aaaatccctg gtcaaccttc tgattataca
    46621 gaagggatgc tcaagtccct tttgactctt gcagatgtca tgccgacagg ctatcatgca
    46681 gcgcgtgttg caaatgttca aaaaggggac aaggttgttg ttatcggtga tggggctgtt
    46741 ggtcaatgtg ctgtcatcgc ggctaagatg cgtggagcat cacaaattat ccttatgagc
    46801 cgtcatgaag accgtcaaaa gatggctatg gagtcaggtg cgacagctgt tgttgcagaa
    46861 cgtggtcaag aaggaattac caaggtgcgt gaaatcctcg gtggaggagc agatgcagca
    46921 cttgaatgtg ttggtacgga ggctgctata gaacaggcgc taggtgttct tcataatgga
    46981 gggcgtatgg gctttgtagg agtcccacac tataataatc gtgctcttgg ttcgacattt
    47041 atgcaaaata tctctgtagc aggtggggca gcttctgcta caacatacga taagcaattt
    47101 ttactaaaag ccgtccttga tggtgatatc aatccaggtc gcgtctttac ttcaagttat
    47161 aaactggaag atatcgacca agcctacaaa gatatggatg aacgtaagac cattaagtct
    47221 atgattataa tcgaataaaa aacgaatagg agttctaaaa ctcctattcg ttttttatgt
    47281 tatcctattc ttgatttagg gtactttctc ttaatgtcag tctggttccc agcatggtca
    47341 ggctagggat tttccgaccg tggaggactt ccttgttaag aatatccata cctgctcggc
    47401 ccatttcttc agtataaact gtaatactag agaggggagg atagacctgt ttggtcagac
    47461 tagtgtcgtt aaaggaaatg aggctgacgc gatctggcag gttgattcca gcttcttgga
    47521 gggcacggag ggcaccgata gctaaactat cgctggctgc gaaaaatgct ggcggaagtt
    47581 ggtctcccaa gctctgaatg gcctccttca tcaagtcata gccagactgg gcagtaaagt
    47641 ttccttgaaa gatcagttca tcatgataga ttccttttgc ttgactgtag tttctgaaat
    47701 tttccagccg cttatcctca atgatttctt cttggtctgt tgtttcttca aggcctgtta
    47761 gaatcccgat acggtccatt ccttgactga ggaaataatc gacaacctgt ttcatagcag
    47821 tgtaaaaatc cgtgataata caggtatgtc ccagggaaag tgtatcgctg tctagaaata
    47881 caagaggctt ttggtattct tcaaaggcag aaatctgagc tcgactaaac tttccgatgc
    47941 agagaatccc aatcacttcc tcgcttaggg taaaagggtg gtcattaaaa tagcgcaaga
    48001 tatcgtagtc caactcttgg gctctttttt ctattcctag gcgaatctgg tagtagtaga
    48061 ggtcgtctag ctccccttgt tcgctgaccc attgaataat ggcaatcttt tgcttgggtt
    48121 tgtgggactc gcctgtcttg aggtgcttgg tgtagcccag ctcttcagca acggttaaaa
    48181 tacggtgtct ggtttcttct gtaacagata ggctctggtc gcggttgagg acacgggata
    48241 cggtcgcgat tgagacagag gctagctgtg caatgtcttt taaggtagcc ataaatcctc
    48301 cttgattagg ttagtatatc atatttttct tctttttact gatattttag taaaatttta
    48361 gtaaaaagga ttgaccttgg gaaattcctt ggatacaata gaaagaaaac gattacacgt
    48421 taagatggct taacggacag tcaaaggaga attcatatgg cacaacatct tactactgaa
    48481 gtccttcgca aagactttct tgctgttttt ggtcaagaag cagaccaaac cttcttttca
    48541 ccaggccgta ttaatttgat tggtgaacac acagactaca acggtgggca cgtttttcct
    48601 gctgctattt ccttgggaac ttacggtgca gctcgtaagc gtgacgacca agtcttgcgt
    48661 ttctactcag ctaactttga ggacaagggc attatcgaag tgcctctcgc tgacctcaag
    48721 tttgaaaaag agcacaactg gaccaattat ccaaaagggg ttcttcattt cttgcaagaa
    48781 gctgggcacg tgattgacaa aggttttgat ttttatgttt atgggaatat cccaaatggt
    48841 gctggcttgt cttcttcagc atccttggag ctcttgacag gagtcgtggc agagcatctc
    48901 tttgatttaa aactagaccg tttggatttg gttaaaatcg gaaaacaaac agaaaacaac
    48961 tttatcggag tcaactctgg tatcatggac cagtttgcta ttggtatggg ggctgaccaa
    49021 cgtgctattt acttagatac caatacttta gaatacgact tggtgccact tgatttgaag
    49081 gacaatgtcg ttgttatcat gaacaccaac aaacgccgtg aattggcgga ctctaaatac
    49141 aatgaacgtc gtgctgagtg tgaaaaagca gtggaagaat tgcaagttgc cttggatatt
    49201 cagaccctgg gtgaattgga cgagtgggcc gttgaccaat atagctattt gattaaagat
    49261 gaaaatcgtt tgaaacgtgc tcgccatgct gtgcttgaaa accaacgtac ccttaaagct
    49321 caagcagccc ttcaagcagg tgatttggaa acatttggtc gtttgatgaa tgcgtcacac
    49381 gtttctctgg agcatgatta tgaagtaact ggtttggaat tggatactct tgttcacaca
    49441 gcttgggcac aagaaggagt tctcggtgct cgtatgacag gggctggttt tggtggctgt
    49501 gccattgcct tggttcaaaa agatactgtt gaggccttta aggaagctgt aggcaaacac
    49561 tacgaggaag tagttggata cgctccaagc ttctatatcg ctgaagttgc aggtggcact
    49621 cgcgtccttg actagtcaaa aggaggctct atagtgatct tagtagataa atttgtaaca
    49681 catgtcattt ctgaaagttc atttgaggaa atggatcgaa tctacctgac caaccgtgtt
    49741 ttggcacgag tgggagaagg tgttttggaa gttgagacca atctggataa attgattgac
    49801 ctcaaggacc agctagttga ggaagccgtt cgattagaga tgattgagga tagtcagact
    49861 gcgcgtgaaa tccttggtac tgaactgatg gacttggtga ctccttatcc gagtcaggtc
    49921 aatcgtgact tctgggaagc ctatgtccac tctcctgagc aagcgataga ggatttttat
    49981 caactcagtc agaaaaatga ttacatcaaa ctcaaggcca ttgctaaaaa tatcgcttat
    50041 cgtgttccat ctgattatgg agaacttgaa attaccatca atctctctaa gcctgaaaag
    50101 gatccgaaag agattgcggt agccaagttg gtgcaagcta gtaattatcc tcagtgtcaa
    50161 ctttgtttag agaatgaggg ttaccatggt cgggtcaacc acccagctcg cagcaaccac
    50221 cggattatcc gttttgaaat ggttggtcag gaatgggggt tccagtattc gccctatgct
    50281 tactttaatg agcattgtat ctttttagat ggccagcatc gtcccatggc cattagtcgt
    50341 cagagttttg aacgtctgtt ggctatcgta gagcagtttc caggatattt tgctggatct
    50401 aatgccgacc tgccgattgt ggggggctct attctaactc atgatcatta tcagggaggc
    50461 cgtcacgtat ttcctatgga attggctccc ttgcaaaaga ccttccgatt tgctggtttt
    50521 gagcaggtca aggctggaat tatcaagtgg ccaatgtctg tcctacgctt gacttcggat
    50581 tccaaagagg atttgattaa cttagctgat aagattttcc aggaatggcg tcagtattca
    50641 gattctagtg tgcagatttt agcagagaca gacgggacac cgcatcacac catcacacca
    50701 atagcgcgta agcgtgatgg acagtttgag ttggacttgg tcttgcgaga caatcagact
    50761 tcagcagagc atcctgatgg catctatcat ccccacaagg atgtccaaca tatcaagaag
    50821 gaaaatatcg gcttgattga ggtcatgggc ttggcaatct tgccaccacg tctgaaagaa
    50881 gaagtagagc aagtcgctag ctatcttgta ggagaagctg ttacagttgc cgattatcat
    50941 caggagtggg cagaccaact caaatcccaa catccagacc taacggataa agaaaaagcc
    51001 cttgcaatcg tcaaggactc tgtgggtgct atctttgtgc gtgtacttga ggatgcagga
    51061 gtctacaagc agacagaaca agggcagaca gcctttatgc gctttgtgga acaggtcgga
    51121 attttactag actaggagct ttctccttgc acagtcctat aaaaagtagt atcatagtgt
    51181 cgtttgaaat atcaggagga aacgatgaaa gactttcatt ttgacgctat atctgccttt
    51241 gaaagttacg aaattgaaaa aatgagagat ggtcacgttg tggtgacgac caaagtagtg
    51301 aactcatcgc tcaactacta tggcaatgcc catggtggct atctattcac gctatgtgac
    51361 caaatcagtg gtttggtggt tatctcgctg ggacttgatg gggtgacact ccaatcctct
    51421 atcaactacc ttaaggcagg aaaactcgac gatgtattga ccattaaagg agaatgtgtc
    51481 catcaaggtc gtacaacctg tgtgatggat gtggatatca ccaatcaaga gggcagaaat
    51541 gtctgtaagg caacctttac catgtttgtc acaggccaaa ggtctgagga aagacgggta
    51601 agtatataaa gaaaggtgag tgggataaat ttatctcact catttttaat ttatgagata
    51661 tgttagaagg aaatataaaa gagagtgatg aaatacaaat gtaataggta catatatgtg
    51721 gtatactagt tatagtagat tttaattgct tatatcgagt gagttctcat attagtgagg
    51781 aatttttaaa tggaattaca ctttaattta gaattagtag aaacctataa aagtaatagc
    51841 caaaaggcac gtatcttaac ggaggattgg gtttatcgtc agtcttattg ccctaattgt
    51901 gggaacaatc ccttaaatca ttttgaaaat aatcggcctg tagcagattt ttactgtaat
    51961 cattgtagtg aggagtttga actaaagagc aaaaaaggaa atttttcatc aacaatcaat
    52021 gatggtgctt atgcaacgat gatgaagcgt gtgcaggcag ataataatcc taatttcttt
    52081 tttttaactt acacaaaaaa ttttgaggta aataactttc ttgtccttcc gaagcaattt
    52141 gttacaccga aatcgattat tcaaagaaaa ccacttgcac caactgctag acgagcaggt
    52201 tggattggtt gtaacattga tttatcacaa gtaccttcta aaggaaggat atttcttgtg
    52261 caagatggac aagttagaga tccagaaaaa gttacaaaag aatttaagca aggtttattt
    52321 ttaaggaaga gctctctgtc atcaagaggt tggacaatag aaattctaaa ttgtatagat
    52381 aagatagagg gttcagaatt tacccttgaa gatatgtatc gttttgaaag tgacctaaaa
    52441 aatatctttg ttaagaacaa tcatatcaaa gaaaagatta ggcaacagct tcaaatatta
    52501 agagacaaag aaataataga atttaaaggt agaggaaagt atcggaaatt atgaaaacga
    52561 aacaacttgt tgcatcagaa gaggtgtatg atttcttaaa agtcatctgg cctgattatg
    52621 aaactgaaag ccgttacgat aacctaagtt taatcgtctg taccttatca gatcccgatt
    52681 gtgtgagatg gttatctgaa aatatgaaat ttggtgacga aaaacaacta gctttgatga
    52741 aggaaaaata tgggtgggaa gtaggagata aattgccaga gtggctacat agctcctatc
    52801 atagattatt gttaataggt gaattattgg aaagcaatct aaaactgaaa aagtatacag
    52861 tagaaattac agaaacttta tcacgtttag taagtataga ggctgaaaat ccagatgaag
    52921 ccgaacgact tgtaagagaa aagtataaga gttgtgaaat tgttcttgat gcagatgatt
    52981 ttcaggacta tgacactagc atatatgaat aggtagatgt ttttattttg tcacacaaaa
    53041 aagaggctcg cacctctttt tcttatttct ttttatgatt taatacggca ttgaggacaa
    53101 tagcgagtag gctggctacg acgattccgt ttgagaagaa catttggaag gctgtcggca
    53161 tgctgacaaa gagattactg ttgttgagac cgacacctgc agcgattgaa acagctgcga
    53221 taaggaagtt gtgttcattg ttagcaaagt caactcgggc gaggatttgc atcccttgaa
    53281 tagatacaaa accaaacatc accagcatgg caccgccgag gacggagctt ggaatgattt
    53341 gggcaagggc accaaactta ggaagcagtc caaggagaac caggaaacca gctgcgtagt
    53401 agattggcag gcgttttttg atgcctgaca atttaaccaa accaacgttt tgtgaaaatc
    53461 cggtgtaagg gaaggtgtta aagattcctc cgagaagtac cgccaaacct tctgcgcggt
    53521 aaccgttgcg aaggcgcgtg ctgtcgattg gatcttttgt gatatcagac aaggctagat
    53581 aaacaccagt tgactcaacc atagacaccg ttgcgatgat acacatcatg acaatagatg
    53641 agatttcaaa ggttggcatc ccaaagtaga gtggagttgg gacatggaca agtggagcta
    53701 ccgcaacagg agagaagtcc accaagccca tagtagcagc aatggcagtt ccaacaacca
    53761 gaccaatcaa aatagagata gacttgataa atcctttggt aaagatgttg atcaagagga
    53821 taatcagaac agtaatagct gcaagcaaga gactttgacc agttggctct ggaacgttat
    53881 ttcccatatt tccaatagcg acagggatca aggttaaacc aatcgtggta ataacagatc
    53941 ctgttacgat agatgggaag agattggcta cttttgagaa gatgcctgaa acaagaacca
    54001 cgtaaatccc agatgcgata agggcaccaa acatagcgcc attaccatgg ctttgcccaa
    54061 tcataatcaa gggagcgacc gactggaatg caactccaag aacgactggg agtccaatcc
    54121 caaagtattt gttgagttgg agttggagga aggttgccac cccacacatg aagatatctg
    54181 tagaaatcag gtaggtcaac tgctcagctg aatagccaag ggctgtcgca atcatgatgg
    54241 gaaccaggat agatcctgag tacatggcta gtaagtgctg caagcccaga acggctgctt
    54301 gcgagtgttt ttcttgagtt tgcattagag atctgcctcc ttaaatacga cctgaccatt
    54361 ttcaaaacga tccaagcgag caagtgatag gacagggtag cctgcttttt caagcaaatc
    54421 acgaccatct tggaaggatt tctcaatcac gataccgata gcttggactg tggcaccggc
    54481 ctgttcgatg atttgaatca agcctttagc agcttggcca ttagcaagga aatcgtcgat
    54541 aatcaaaacc ttgtcctctg gtgagaggaa ttttccagcg atagaaacag tgctggtcac
    54601 ctgcttggta aaggagtaga cttgagcagt taagatgcct tcgttcatgg tgatgttctt
    54661 agcttttttg gcgaaaatca tgggaacgtt taaggcttca gctgtaaaaa cggctggggc
    54721 aatacccgac gcttcaatgg ttacgacctt ggtaatgcca gtagcagcaa atttttccgc
    54781 aaaaacctta ccaatctctc gcatcaagct aaagtcaact tggtgggtta aaaaggaatc
    54841 taccttgagg atgttatcac ccaagatatg cccatccttg aggatgcgct cttctaataa
    54901 tttcataaga cctcctaaag tctaaaagtt aatttacttg ttgtttaaat atttctatag
    54961 tgatcccttt tgctaatact atatatttga taaaactatt acgagcgaag cgagtcttat
    55021 caaatatttc ccgttgtagt ggtatcatag acaataatct tgttattgtc tatgacggga
    55081 tttttgagag taaaatagtt cggggaacta ttttagccta agcctagaaa tgaaagagct
    55141 aggggctcaa aaattaggga tgaaattccc tggattcctg aaattattca caggataatt
    55201 tcacctcccg tccgcactaa ttaagggaaa tattaaaaat attcaaaaaa gacctactta
    55261 atctctaagt aagtccccta aatagacatg gcaaaaacgg ccatatctca ctgctgactt
    55321 acttattgtt aggtgttccg gcaccttgta gaaacgtcgt gccaattcac gacataaaca
    55381 agtaaaacga tattcaattt taaataggct tgagccaatg tttttatttt acactaaata
    55441 actttagaaa tcaactattt tgttagtgtt ttggtttaaa aagcgaacaa aaataagaga
    55501 gggtgaacaa aaactccatt gtaagctaac atttatacta aatgaaaatc aaagagcaaa
    55561 ctaggaagct atccacaacc tcaaaacact gttttgaggt tgtggataga attgacagag
    55621 tcagtatcat atacctacgg taaggcgacg ttgacgtggc ttgaagagat tttcgaagag
    55681 tattagaaga tttttccatc ataaaaggca tactatcaag tttttagaca cctgacaata
    55741 tgcctttttc taactttaaa gacttttccc aatttttatt attctactcg ctaaatctta
    55801 aaaaatagcc atctggatcc aaaactgcaa atttatgagg atagatatag ggatcactga
    55861 cacgaaactt tcttttggtc aagagacgat aaataggata gtttgcctcc atcacttttt
    55921 gatagagttt tggaacatcc tttatgccaa aggagagatt gactccacga ccaaaggggt
    55981 aggtcagttc agctagttga tcctttgttc cctcctctaa cattagttga cactcttcaa
    56041 gagaaagaga aagttttctt ctggacgtta gtattcaatc ctaaaaccca gtaaaccaca
    56101 gtagaaggac cgggactgtt cgatattcga tacaagaaac tcgggaatga ccgcattgta
    56161 gtccatatag aaaatcctta caagtcaatt tccaagacaa tcggtgtatg gtcttggcga
    56221 gcacctgagt caatcatatc agatttagtg accttgtcag cgatacggtt acttgtgagc
    56281 cagtagtcga ttctccagcc tgtattgttg attttagaag ttttgctgcg ttgtgcccac
    56341 caagtgtagc gttcaggaac atcgccatga acatggcgga aggtgtctgt aaatccagtt
    56401 gccaaaaggt tggtaaatcc agcacgttcc tcgtcagtaa atccaggtga acggcggttg
    56461 ctagcaggat ttgcaaggtc gatttcattg tgggctacgt tgtagtcacc ggtcgcaagg
    56521 actggttttt ctttgtctag ttcagccaaa tactcagcat atttaacatc ccagacttgg
    56581 cgttcttcca agcgtttgag accgtcacca gcgtttggag tgtaaacttg ggttacgaaa
    56641 aatgcatcaa attctagagt gatgatacgg ccttccaagt ccatggtaga aggggcaccg
    56701 atttctggaa aactgatcgt aggcgtaagt tctttcttat aaaggaacat ggttccagca
    56761 tagcctttac gggcaggctc ttgggaagag cgccatgtgt tttcgtagcc tgggaagagt
    56821 tcttctaaaa tttccacgtg tttctttgta ggtcctttgg cagaaagctt ggtttcttgg
    56881 atagcaatga tatcagcatt ttcagcgacc aaggtttgta ggacttcttg ggacaatttg
    56941 gcacgagctg agtcactagt tagggcagcg tttagggaat caatattcca tgagataagt
    57001 ttcataaagt tacctttttc attcagatta tagattttat tataccaaaa aaagatctat
    57061 ttccccaacg tatggtttga aaaattactc tctttcgttt ataattaaga atgattttat
    57121 gaaagggagt gaaaatatat gaaattctac tcttatgact atgtactcag ccaaatcggt
    57181 cagcaaaatg gtatcatggt tggctttggg attgttctat tagctgtgac agtttttttt
    57241 gctttcaagg cataccatga taaaaaggga accgaatttc gtgagttggt catgatttca
    57301 gctctggcct tatttagctc tgcttttggt cagcatcacg acttatcaaa acaatcaagt
    57361 ttctaacaat aaatttcaaa cttcacttca tttcatcgag gttgtttcca aagatttggg
    57421 agtagacaag tcagaagtct atgttaatac ttccacaaac acagatggcg cacttatcaa
    57481 ggtgggagat cgctattatc gtgccctaaa cggaagtgag ccagacaagt acctgttaga
    57541 gaaagtcgaa ttgtataaga cagacgcaat tgaactggtg gatgtgaaca aatgacactt
    57601 aattatatcg aaattttaat caaactggtc ttgactctca aatggctcaa caacaatgtt
    57661 cactttgtga aacgtttgat tgatggtaag ccaactctcc ttatcaaaaa tgggaatatt
    57721 gacccagaag cctgtcgttc agttggtttg tctgcatcgg atgtagccct caaacttcgt
    57781 agccaaggga ttttccagat gaagcaagtc aaacgagctg tgcaagagca aaatgggcaa
    57841 cttatcgttg tgcaaatggg agatgaaaat cctaagtatc cagttgtgac tgacggtgtg
    57901 attcaagtag atgtcttgga atcgattggt cgtagcgaag agtggttgct tgataacctc
    57961 agtaaacaag ggcatgacaa tgtagccaat atctttattg ctgaatatga caagggtgct
    58021 gttacagtcg taacttatga aaaagaaaaa cctggggtct tgtactcttc gaaaatctct
    58081 tcaaaccgcg tcaacgtcgc cttgccgtat gtaggttact gacttcgtca gttctatcta
    58141 caacctcaaa gcagtgcttt gagcagcctg cggctagttt cctagtttgc tctttgattt
    58201 tcattgagta ttggcctcag gtttccattt gcaatcagaa agggatttta tgtccattat
    58261 tcaaaaactt tggtggtttt tcaagttaga aaaacgccgt tatctagtcg gaattgtggc
    58321 cctggtcttg gtttccgtcc tcaatctcat tcctcctatg gttatggggc gggttattga
    58381 tgccatcaca tcggggcaat taacccagca ggacctcctt cttagcctat tttacttgct
    58441 acttgcagcc tttggtatgt actatttgcg ctatgtgtgg cgtatgtata tccttgggac
    58501 ctcttattgc ttgggacaga tcatgcggtc tcgcttgttt aagcatttca caaaaatgtc
    58561 gtcagccttt tatcaaacct atcggacggg tgatctgatg gcacacgcaa ccaatgatat
    58621 caatgccttg actcgtttag caggtggcgg tgtcatgtct gcggtggatg cctctatcac
    58681 ggctctggtg actttgttga ccatgctctt tagcatctca tggcagatga ctcttgttgc
    58741 cattctcccc ctacctttca tggcctatgc gactagtcgc ctagggagaa agactcataa
    58801 ggcctttggc gaatcccaag ctgctttttc tgaactcaat aacaaggtac aggagtccgt
    58861 atcaggtatc aaagtgacca agtctttcgg ttatcaggca gacgagttga agtcttttca
    58921 ggcagtcaat gaattaacct tccaaaagaa cctgcaaacc atgaaatatg atagtctctt
    58981 tgaccctatg gttctcttgt ttgttggttc gtcctatgtt ttaacgcttt tggttggctc
    59041 cttggtggtt caggaagggc agattacagt tgggaatcta gtcaccttta tcagctattt
    59101 ggatatgctg gtctggcctc ttatggccat cggcttcctc tttaatacta ctcagcgagg
    59161 gaaggtttct taccagcgga ttgaaaatct tttgtctcag gaatctcctg tacaagaccc
    59221 ggagtttcct ctggatggta ttgaaaatgg gcgtttggag tatgccattg acagctttgc
    59281 ttttgaaaat gaggaaacac tgacggatat tcactttagt ttggcaaaag ggcaaacact
    59341 gggcttggtt gggcagacag gctctgggaa aacgtcctta atcaagctcc tcttgcgtga
    59401 atacgatgtg gataagggcg ccatttatct aaacggtcac gatattcggg actatcgtct
    59461 gacagacctt cgcagtctca tgggctatgt tcctcaggac cagtttcttt ttgcgacttc
    59521 aatcctagac aatatccgct ttggcaatcc taacttgccc ctttcagcgg tcgaggaagc
    59581 tactaagcta gcccgagttt accaagatat tgtagacatg cctcaaggat ttgatacgct
    59641 gattggtgaa aaaggagtca gtctttctgg tggtcaaaag caacggttgg ctatgagtcg
    59701 ggctatgatt ttagaccctg atatcttgat tttggatgat tccttatccg ccgtagatgc
    59761 caagacagag tatgcgatta tcgacaacct caaggagacg cgaaaggaca agacaaccat
    59821 tatcactgcc catcgcctga gtgcagttgt ccatgcagat ttgattttag tcctgcaaaa
    59881 tggtcaaatt atcgaacgag gcaggcacga agacttgcta gctttggatg gctggtatgc
    59941 ccaaacctac cagtctcagc agctggaaat gaagggagaa gaagatgcag aataaacaag
    60001 aacaatggac tgtattgaag cgcttgatgt cctatctcaa gccttatgga ctccttacct
    60061 ttttggcact cagttttctc ctagcgacga cggtcattaa aagtgtcatt ccccttgtgg
    60121 cttcccactt tatcgaccag tatctcagca atcttaacca actagccgtg accgttttgc
    60181 tggtctacta tggtctctac atcctacaaa ctgtagttca gtatgtcggc aatcttctct
    60241 ttgcgcgcgt gtcttacagt attgttaggg atattcgtcg ggatgccttt gccaatatgg
    60301 agaaactggg catgtcttac tttgacaaga cgccagcagg ttctatcgtt tctcgtttga
    60361 ccaacgatac cgagacgatt agtgatatgt tttctgggat tttatccagc tttatctcag
    60421 cagtttttat ctttctgaca accctttata ccatgttggt gctggatttt cgtttgacgg
    60481 ctttagtctt gctctttctc cccttgattt tccttttggt caatctctat cgaaaaaagt
    60541 cagtgaaaat catcgagaaa accagaagtc tcttgtcaga tatcaatagt aagctggcag
    60601 agaatatcga gggaatcagg attattcagg cttttaatca agagaagcgc ctgcaggcag
    60661 aatttgatga aatcaaccaa gagcacttgg cctatgccaa ccgttctgta gccttggatg
    60721 ccctcttttt gagacctgcc atgagtttgt tgaaacttct aggttatgct gtcttgatga
    60781 cctattttgg ctatcgtggt ctttctatcg ggataacggc cgggaccatg tatgctttta
    60841 tccagtacat caaccgcctc tttgaccctt tgattgaggt gacgcaaaac ttttcaacac
    60901 tgcaaacgtc tatggtttct gcaggccgtg tctttgcctt aatcgatgag aggacctatg
    60961 agccccttca agaaaatggg caagccaaag tcaaagaagg caatatccgt tttgaacatg
    61021 tgtgtttctc gtatgacggt aaacatccga ttctggatga catttctttc tctgttaata
    61081 agggtgaaac cattgccttt gtaggtcata caggttcagg gaaatcgtct attatcaatg
    61141 tcctcatgcg cttttatgaa ttccagtcag ggagagttct cttggatgat gtggatatca
    61201 gggatttcag tcaagaagag ctgagaaaaa acatcggttt ggttttacag gaacccttcc
    61261 tctatcatgg aactattaag tccaatatcg ccatgtacca agaaatcagt gatgagcagg
    61321 ttcaggctgc ggcagccttt gtggatgcag attcctttat tcaagaactt cctcaggggt
    61381 acgactcccc tgtttccgag cgtggttcga gcttctctac tgggcagcgc cagcttcttg
    61441 cctttgctag aacagtcgcc agccagccta aaatcctgat tttggatgaa gcgacagcca
    61501 atattgactc tgaaacagaa agtttggttc aagcttctct ggcgaagatg agacagggcc
    61561 gaacaactat tgctatcgct caccgccttt ctactattca agacgccaac tgcatctatg
    61621 tcttggataa gggacgcatt atcgagagtg gaacccatga ggaactcttg actctgggag
    61681 gaacctatca caagatgtat agtttgcagg caggggccat ggcctatact ctttgaaaat
    61741 ctctttaaac catgtcagct ttatctgcaa tctcaaagct gtactttgat tttcattgag
    61801 tactagaagg aaatccttca aattacagat ttctttcacc gccttttcca ttttgtggta
    61861 taatgaaaaa tgttgacaaa tagtataata aaaacaaagg agaacagcat gctgaaatgg
    61921 gaagacttgc ctgtggaaat gaaatcaagc gaggttgagt cttactacca gcttgtctct
    61981 aaaaggaagg gttcgctgat tttcaagcgt tgcttggact gggttttggc cttggtctta
    62041 ctggttctaa cttctcccat ctttctcatc ttgagcattt ggatcaagtt ggatagcaag
    62101 gggccagtga tttacaagca agagcgtgtg acccagtaca accgtcggtt caagatttgg
    62161 aagttccgta ccatggtgac ggatgcggat aaaaaaggaa gtctggtgac ttctgctaac
    62221 gatagccgta ttaccaaggt tggaaatttc atccgacgtg tccgtttgga cgaactgcct
    62281 cagttggtca atgtccttaa aggtgagatg tcctttgtcg gtacacgacc tgaagtgcca
    62341 cgttatacag agcagtatag ccctgaaatg atggcaacct tgctcttgca agcaggaatt
    62401 acctctccag ccagcatcaa ctacaaggat gaggacacca tcatcagtca aatgacggag
    62461 aaaggtctgt cagttgatca ggcctatgtg gagcatgttc ttcctgaaaa gatgcgctat
    62521 aacctcgcct atctccgaga gtttagtttc tttggggaca tcaaaatcat gtttcaaacc
    62581 gtgtttgagg tactaaaata aagtagtcat aagaaaatga gtacagataa aaggagcaaa
    62641 tcaatgccaa attacaatat tccattttca ccgcctgata tcacagaagc agaaattgct
    62701 gaagtagcgg ataccctgcg ttctggttgg atcacaacag gtcctaaaac aaaagaactg
    62761 gagcgccgct tgtctcttta cacacagaca cctaagactg tttgtctcaa ctctgcgaca
    62821 gccgctctgg agttgatttt acgcgttttg gaagtgggac ctggtgatga agtcatcgtt
    62881 ccagccatga cctatacggc ttcatgtagt gtcattacgc acgtgggagc aacccctgtc
    62941 atggtggata tccaagcaga tacgtttgag atggactatg acctgcttga gcaagctatc
    63001 actgagaaaa ctaaggtgat tatcccagta gagctcgcag ggattgtttg cgattatgac
    63061 cgtttgttcc aagtcgtgga gaaaaaacgt gacttcttta ccgcttcaag caagtggcaa
    63121 aaggccttta accgtattgt cattgtctct gatagtgccc acgctttggg atctacttat
    63181 aaaggacaac cttctggttc tatcgctgat tttacttcct tctcattcca tgctgttaag
    63241 aactttacaa cggcagaagg tggaagtgcg acttggaaag ccaatccagt gattgatgac
    63301 gaagagatgt acaaggaatt ccaaatcctt tcccttcacg ggcaaactaa ggatgctctt
    63361 gccaagatgc aactggggtc atgggaatac gatatcgtta caccagccta taagtgcaac
    63421 atgaccgata tcatggcttc acttggtttg gtacaattgg accgctatcc aagtttgttg
    63481 caacgccgta aggacattgt ggaccgctat gatagtggtt ttgcaggttc tcgcatccat
    63541 cctttggcac acaagactga aactgtcgaa tcttcacgcc acctctacat cacccgtgta
    63601 gaaggagcaa gcctagaaga acgcaacctc atcatccaag aattggctaa agcaggaatt
    63661 gcaagtaatg ttcactacaa accgcttcct ctcttgacag cctataagaa tcttggattt
    63721 gatatgacga actatcctaa ggcctatgcc ttctttgaga atgaaattac cctccctctt
    63781 catactaaat taagcgatga agaagtagac tatatcattg agactttcaa aacagtttct
    63841 gaaaaagtgc taactttatc aaaaaaatga caaactacag tcaagcgaaa gtgatcctgt
    63901 ccctaaaaag tctaattgag tgtaaaaact gttgttttca attgataata gtttacacct
    63961 gtagttgagg cctctttctc ctcagagaga gaatttttat aggattttcc tttcttgtgg
    64021 gagtcccgtg gtttgaaata agatgtgagc aatttggtat agcatttaga atccttacta
    64081 gacatcattt agaaaatcta gtgtcttgtt ctagttttca attcacccta ttttttgaaa
    64141 gacgtgagtt tccatgagtg agattgtgga aactagcgtc ttttttttgt tttcagaata
    64201 ttgttcaaag ttttgtgcct gtctttcatg ttctagtcat tcttttgcat gatagaattt
    64261 atagcatgtt gatattataa taacgcaaat atcccatatg tttagtgatg cttgctatac
    64321 atcattagat ctcctgcgag acaatctata aaacacttgt ctacgattac ctatatgccc
    64381 tattccagta ttttagaagc actgcatcta tttttatcga ggttaaatct agcttttata
    64441 gaaggtctat ttaagaaata tattgtagtg ttttagtttc aatccgccat atgagcgata
    64501 ttcaggtaaa tatccctggc gaatgcttgt atgacaaggt atttgttctt tcatttataa
    64561 tttacaacat atcaacaaat ttaaatatag taaatgggat attttatatt caagctaaga
    64621 aagatagcat cacttttgaa tggaaggcta aagagcaaac taggaagttg gccatagata
    64681 gctcaaaacc ctgctttgag gttgtagata tagtaaaatg aaacaagaat aggacaaatt
    64741 gatcgggaca gttaaatcga tttctaacaa tgttttagaa gtagaggtgt actattatag
    64801 tttcagtcta ctatagaact gaccaagtca gtaacctaga cttagggcaa ggcggcactg
    64861 acctagtttg aagagatttc cgaagagtat aaattttaat attttcttgt gttattcctt
    64921 gacaattcaa tttggaaaat atatgataaa gataatgaca gcggtgtcat tctatctatt
    64981 ttaagaaaag taataatcaa ttgttaaaaa tagtaaaaaa attggaggtt ctgatgaaat
    65041 attttgttcc taatgaggta ttcagtattc gtaaattaaa ggtggggact tgctcggtac
    65101 tattggcaat ttcaattttg ggaagccaag gtattttatc ggatgaagtt gttactagtt
    65161 cttcaccgat ggctacaaaa gagtcttcta atgcaattac taatgattta gataattcac
    65221 caactgttaa tcagaatcgt tctgctgaaa tgattgcctc taattcaacc actaatggtt
    65281 tagataattc gttaagtgtt aatagtatca gctctaatgg tactattcgt tccaattcac
    65341 aattagacaa cagaacagtt gaatctacag taacatctac taatgaaaat aagagttata
    65401 aggaagatgt tataagtgac agaattatca aaaaagaatt tgaagatact gctttaagtg
    65461 taaaagatta tggtgcggta ggtgatggga ttcatgatga tcgacaagca attcaagatg
    65521 caatagatgc tgcagctcaa gggctaggtg gaggaaatgt atattttcct gaaggaactt
    65581 atttagtaaa agaaattgtt tttttaaaaa gtcatacaca cttagaattg aatgagaaag
    65641 ctacaattct aaatggtata aatattaaga atcacccttc cattgttttt atgacaggtt
    65701 tatttacgga tgatggtgcg caagtagaat ggggcccaac agaagatatt agttattctg
    65761 gtggtacgat tgatatgaac ggtgctttga atgaagaagg aactaaagca aaaaatctac
    65821 cacttataaa ttcttcaggt gcatttgcta ttgggaattc aaataacgta actataaaaa
    65881 atgtaacatt caaggatagt tatcaagggc atgctattca aattgcaggt tcgaaaaatg
    65941 tattagttga taattctcgt tttcttgggc aagccttacc caaaacgatg aaggatgggc
    66001 aaatcataag taaggagagc attcagattg aaccattaac tagaaaaggt tttccttatg
    66061 ccttgaatga tgatgggaaa aaatctgaaa atgtgactat tcaaaattcc tattttggca
    66121 aaagtgataa atctggggaa ttagtaacag caattggcac acactatcaa acattgtcga
    66181 cacagaaccc ctctaatatt aaaattttaa ataatcattt tgataacatg atgtatgcag
    66241 gtgtacgttt tacaggattc actgatgtat taatcaaagg aaatcgcttt gataagaaag
    66301 ttaaaggaga gagtgtacat tatcgagaaa gcggagcagc tttagtaaat gcttatagct
    66361 ataaaaacac taaagaccta ttagatttaa ataaacaggt ggttatcgcc gaaaatatat
    66421 ttaatattgc cgatcctaaa acaaaagcga tacgagttgc aaaagatagt gcagaatatt
    66481 t
//
LOCUS       DAVLVF010000003        64601 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVLVF010000003 DAVLVF010000000
VERSION     DAVLVF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 64601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64601
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.003"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(168..242)
                     /locus_tag="VYN66_000123"
                     /pseudo
     CDS             complement(168..242)
                     /locus_tag="VYN66_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            313..969
                     /locus_tag="VYN66_000124"
     CDS             313..969
                     /locus_tag="VYN66_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV1494217.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWHNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGDLLPNPEIFGLDFALVGMFIGI
                     FASQFQMMQRRISVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGMVLDGQ
                     "
     gene            959..1168
                     /locus_tag="VYN66_000125"
     CDS             959..1168
                     /locus_tag="VYN66_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV1494218.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPFILVKYKGLPTIVERFLKFL
                     PVSIIFALILSRVVTGKVGNLPQIK"
     gene            1386..2216
                     /locus_tag="VYN66_000126"
     CDS             1386..2216
                     /locus_tag="VYN66_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1494219.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2370..3224
                     /locus_tag="VYN66_000127"
     CDS             2370..3224
                     /locus_tag="VYN66_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1494220.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3324..4697
                     /locus_tag="VYN66_000128"
     CDS             3324..4697
                     /locus_tag="VYN66_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1494221.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYHTHIELVE
                     ELIRSYE"
     gene            4690..5751
                     /locus_tag="VYN66_000129"
     CDS             4690..5751
                     /locus_tag="VYN66_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV1494222.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            5753..6445
                     /locus_tag="VYN66_000130"
     CDS             5753..6445
                     /locus_tag="VYN66_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1494223.1"
                     /translation="MESLIQTYLPNVYKMGWSGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAIPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6474..7043)
                     /locus_tag="VYN66_000131"
     CDS             complement(6474..7043)
                     /locus_tag="VYN66_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1494224.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7178..7792
                     /locus_tag="VYN66_000132"
     CDS             7178..7792
                     /locus_tag="VYN66_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1494225.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7794..9440
                     /locus_tag="VYN66_000133"
     CDS             7794..9440
                     /locus_tag="VYN66_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1494226.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9452..10738
                     /locus_tag="VYN66_000134"
     CDS             9452..10738
                     /locus_tag="VYN66_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1494227.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVSYIKETLISFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10790..11323
                     /locus_tag="VYN66_000135"
     CDS             10790..11323
                     /locus_tag="VYN66_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1494228.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            11401..11871
                     /gene="nrdI"
                     /locus_tag="VYN66_000136"
     CDS             11401..11871
                     /gene="nrdI"
                     /locus_tag="VYN66_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV1494229.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12164..13426)
                     /locus_tag="VYN66_000137"
     CDS             complement(12164..13426)
                     /locus_tag="VYN66_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV1494230.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(13736..14194)
                     /locus_tag="VYN66_000138"
     CDS             complement(13736..14194)
                     /locus_tag="VYN66_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV1494231.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(14191..14631)
                     /locus_tag="VYN66_000139"
     CDS             complement(14191..14631)
                     /locus_tag="VYN66_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1494232.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            14987..16936
                     /gene="mutL"
                     /locus_tag="VYN66_000140"
     CDS             14987..16936
                     /gene="mutL"
                     /locus_tag="VYN66_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV1494233.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(17261..17728)
                     /locus_tag="VYN66_000141"
     CDS             complement(17261..17728)
                     /locus_tag="VYN66_000141"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV1494234.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(17729..18934)
                     /locus_tag="VYN66_000142"
     CDS             complement(17729..18934)
                     /locus_tag="VYN66_000142"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV1494235.1"
                     /translation="MEYRKIQETLEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLIERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(18954..19589)
                     /gene="ribE"
                     /locus_tag="VYN66_000143"
     CDS             complement(18954..19589)
                     /gene="ribE"
                     /locus_tag="VYN66_000143"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV1494236.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSINGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(19574..20674)
                     /gene="ribD"
                     /locus_tag="VYN66_000144"
     CDS             complement(19574..20674)
                     /gene="ribD"
                     /locus_tag="VYN66_000144"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV1494237.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(20808..20922)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            21079..21672
                     /gene="ruvA"
                     /locus_tag="VYN66_000145"
     CDS             21079..21672
                     /gene="ruvA"
                     /locus_tag="VYN66_000145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV1494238.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            21682..22245
                     /locus_tag="VYN66_000146"
     CDS             21682..22245
                     /locus_tag="VYN66_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV1494239.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTETELEAMLENPAIIRNRAKLFATRANAQ
                     VLLQLQAEYGSFDAYLWSFVEGKIVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            22242..22919
                     /locus_tag="VYN66_000147"
     CDS             22242..22919
                     /locus_tag="VYN66_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1494240.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSVIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVRL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(23056..24087)
                     /locus_tag="VYN66_000148"
     CDS             complement(23056..24087)
                     /locus_tag="VYN66_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV1494241.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(24342..25025)
                     /locus_tag="VYN66_000149"
     CDS             complement(24342..25025)
                     /locus_tag="VYN66_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV1494242.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILTSMFLWLLVFFATSFLPASLNPVLDPLPLAIIGVALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(25037..25981)
                     /locus_tag="VYN66_000150"
     CDS             complement(25037..25981)
                     /locus_tag="VYN66_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1494243.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            26113..28944
                     /gene="uvrA"
                     /locus_tag="VYN66_000151"
     CDS             26113..28944
                     /gene="uvrA"
                     /locus_tag="VYN66_000151"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV1494244.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            28937..29998
                     /locus_tag="VYN66_000152"
     CDS             28937..29998
                     /locus_tag="VYN66_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1494245.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            30130..30528
                     /gene="spx"
                     /locus_tag="VYN66_000153"
     CDS             30130..30528
                     /gene="spx"
                     /locus_tag="VYN66_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV1494246.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            30594..31163
                     /locus_tag="VYN66_000154"
     CDS             30594..31163
                     /locus_tag="VYN66_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1494247.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            31250..31516
                     /locus_tag="VYN66_000155"
     CDS             31250..31516
                     /locus_tag="VYN66_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV1494248.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            31520..31939
                     /gene="ruvX"
                     /locus_tag="VYN66_000156"
     CDS             31520..31939
                     /gene="ruvX"
                     /locus_tag="VYN66_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV1494249.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            31955..32260
                     /locus_tag="VYN66_000157"
     CDS             31955..32260
                     /locus_tag="VYN66_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV1494250.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            32518..33768
                     /locus_tag="VYN66_000158"
     CDS             32518..33768
                     /locus_tag="VYN66_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1494251.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            33765..34307
                     /locus_tag="VYN66_000159"
     CDS             33765..34307
                     /locus_tag="VYN66_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV1494252.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            34453..35985
                     /gene="cls"
                     /locus_tag="VYN66_000160"
     CDS             34453..35985
                     /gene="cls"
                     /locus_tag="VYN66_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV1494253.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            36243..37781
                     /locus_tag="VYN66_000161"
     CDS             36243..37781
                     /locus_tag="VYN66_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1494254.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWGLHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            37901..40108
                     /gene="nrdD"
                     /locus_tag="VYN66_000162"
     CDS             37901..40108
                     /gene="nrdD"
                     /locus_tag="VYN66_000162"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV1494255.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            40120..40260
                     /locus_tag="VYN66_000163"
     CDS             40120..40260
                     /locus_tag="VYN66_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494256.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            40304..40810
                     /locus_tag="VYN66_000164"
     CDS             40304..40810
                     /locus_tag="VYN66_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1494257.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            40803..41393
                     /gene="nrdG"
                     /locus_tag="VYN66_000165"
     CDS             40803..41393
                     /gene="nrdG"
                     /locus_tag="VYN66_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV1494258.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            41401..42018
                     /locus_tag="VYN66_000166"
     CDS             41401..42018
                     /locus_tag="VYN66_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV1494259.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRYASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            42284..42592
                     /gene="rpsJ"
                     /locus_tag="VYN66_000167"
     CDS             42284..42592
                     /gene="rpsJ"
                     /locus_tag="VYN66_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV1494260.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            42809..43435
                     /gene="rplC"
                     /locus_tag="VYN66_000168"
     CDS             42809..43435
                     /gene="rplC"
                     /locus_tag="VYN66_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV1494261.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            43460..44083
                     /gene="rplD"
                     /locus_tag="VYN66_000169"
     CDS             43460..44083
                     /gene="rplD"
                     /locus_tag="VYN66_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV1494262.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            44083..44379
                     /locus_tag="VYN66_000170"
     CDS             44083..44379
                     /locus_tag="VYN66_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV1494263.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            44397..45230
                     /gene="rplB"
                     /locus_tag="VYN66_000171"
     CDS             44397..45230
                     /gene="rplB"
                     /locus_tag="VYN66_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV1494264.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            45334..45615
                     /gene="rpsS"
                     /locus_tag="VYN66_000172"
     CDS             45334..45615
                     /gene="rpsS"
                     /locus_tag="VYN66_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV1494265.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            45627..45971
                     /gene="rplV"
                     /locus_tag="VYN66_000173"
     CDS             45627..45971
                     /gene="rplV"
                     /locus_tag="VYN66_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV1494266.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            45984..46637
                     /gene="rpsC"
                     /locus_tag="VYN66_000174"
     CDS             45984..46637
                     /gene="rpsC"
                     /locus_tag="VYN66_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV1494267.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            46641..47054
                     /gene="rplP"
                     /locus_tag="VYN66_000175"
     CDS             46641..47054
                     /gene="rplP"
                     /locus_tag="VYN66_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV1494268.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            47064..47270
                     /locus_tag="VYN66_000176"
     CDS             47064..47270
                     /locus_tag="VYN66_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV1494269.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            47295..47555
                     /gene="rpsQ"
                     /locus_tag="VYN66_000177"
     CDS             47295..47555
                     /gene="rpsQ"
                     /locus_tag="VYN66_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV1494270.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            47581..47949
                     /gene="rplN"
                     /locus_tag="VYN66_000178"
     CDS             47581..47949
                     /gene="rplN"
                     /locus_tag="VYN66_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV1494271.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            48027..48332
                     /gene="rplX"
                     /locus_tag="VYN66_000179"
     CDS             48027..48332
                     /gene="rplX"
                     /locus_tag="VYN66_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV1494272.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            48356..48898
                     /gene="rplE"
                     /locus_tag="VYN66_000180"
     CDS             48356..48898
                     /gene="rplE"
                     /locus_tag="VYN66_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV1494273.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            48916..49185
                     /gene="rpsN"
                     /locus_tag="VYN66_000181"
     CDS             48916..49185
                     /gene="rpsN"
                     /locus_tag="VYN66_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV1494274.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            49470..49868
                     /gene="rpsH"
                     /locus_tag="VYN66_000182"
     CDS             49470..49868
                     /gene="rpsH"
                     /locus_tag="VYN66_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV1494275.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            50060..50596
                     /gene="rplF"
                     /locus_tag="VYN66_000183"
     CDS             50060..50596
                     /gene="rplF"
                     /locus_tag="VYN66_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV1494276.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            50680..51036
                     /gene="rplR"
                     /locus_tag="VYN66_000184"
     CDS             50680..51036
                     /gene="rplR"
                     /locus_tag="VYN66_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV1494277.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADSPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            51054..51548
                     /gene="rpsE"
                     /locus_tag="VYN66_000185"
     CDS             51054..51548
                     /gene="rpsE"
                     /locus_tag="VYN66_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV1494278.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            51520..51744
                     /gene="rpmD"
                     /locus_tag="VYN66_000186"
     CDS             51520..51744
                     /gene="rpmD"
                     /locus_tag="VYN66_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV1494279.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            51889..52329
                     /gene="rplO"
                     /locus_tag="VYN66_000187"
     CDS             51889..52329
                     /gene="rplO"
                     /locus_tag="VYN66_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV1494280.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            52342..53652
                     /gene="secY"
                     /locus_tag="VYN66_000188"
     CDS             52342..53652
                     /gene="secY"
                     /locus_tag="VYN66_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV1494281.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            53803..54441
                     /locus_tag="VYN66_000189"
     CDS             53803..54441
                     /locus_tag="VYN66_000189"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV1494282.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            54558..54776
                     /gene="infA"
                     /locus_tag="VYN66_000190"
     CDS             54558..54776
                     /gene="infA"
                     /locus_tag="VYN66_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV1494283.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            54801..54917
                     /gene="rpmJ"
                     /locus_tag="VYN66_000191"
     CDS             54801..54917
                     /gene="rpmJ"
                     /locus_tag="VYN66_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV1494284.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            54935..55300
                     /gene="rpsM"
                     /locus_tag="VYN66_000192"
     CDS             54935..55300
                     /gene="rpsM"
                     /locus_tag="VYN66_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV1494285.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            55318..55701
                     /gene="rpsK"
                     /locus_tag="VYN66_000193"
     CDS             55318..55701
                     /gene="rpsK"
                     /locus_tag="VYN66_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV1494286.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            55744..56679
                     /locus_tag="VYN66_000194"
     CDS             55744..56679
                     /locus_tag="VYN66_000194"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV1494287.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            56691..57077
                     /gene="rplQ"
                     /locus_tag="VYN66_000195"
     CDS             56691..57077
                     /gene="rplQ"
                     /locus_tag="VYN66_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV1494288.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            57342..57608
                     /locus_tag="VYN66_000196"
     CDS             57342..57608
                     /locus_tag="VYN66_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV1494289.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            57618..58955
                     /locus_tag="VYN66_000197"
     CDS             57618..58955
                     /locus_tag="VYN66_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV1494290.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALTETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            59199..59891
                     /locus_tag="VYN66_000198"
     CDS             59199..59891
                     /locus_tag="VYN66_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1494291.1"
                     /translation="MVKVRLYLIRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(59994..61630)
                     /locus_tag="VYN66_000199"
                     /pseudo
     CDS             complement(59994..61630)
                     /locus_tag="VYN66_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(61620..62648)
                     /locus_tag="VYN66_000200"
     CDS             complement(61620..62648)
                     /locus_tag="VYN66_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494292.1"
                     /translation="MNITIPKGSLFTLLGPSGCGKTTLLRMIAGFNSIKDGEFYFDDT
                     KINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLKQKKVPKEELIQQTNKYLELMQI
                     AQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEPLSNLEAKLRLDMRQAIREIQH
                     EVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRPKELYHKPANEFVATFIGRTNI
                     IPANLEKRSDGAYIVFSDGYALRMPALDQVEEQAIHVSIRPEEFIKDESGDIEGTISD
                     SVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKDNRIRLRINTQKLNIFSADGSQNL
                     IKGVNHGM"
     gene            complement(62723..63829)
                     /locus_tag="VYN66_000201"
                     /pseudo
     CDS             complement(62723..63829)
                     /locus_tag="VYN66_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100970.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(64061..64258)
                     /locus_tag="VYN66_000202"
     CDS             complement(64061..64258)
                     /locus_tag="VYN66_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1494293.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(64333..>64601)
                     /locus_tag="VYN66_000203"
     CDS             complement(64333..>64601)
                     /locus_tag="VYN66_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV1494294.1"
                     /translation="FSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPF
                     HQFGENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
BASE COUNT        19347 a        12108 c        14240 g        18906 t
ORIGIN      
        1 ttcaatctgc tatataatag tccatgctgt ggctggattc ttttcagcct acttatactc
       61 ttcgaaactc tcttcaaacc acgtcagctt caccttgccg tatatgtgtt actgacttcg
      121 tcagttctat ccacaacctc aaaacagtgt tttgagctga cttcatcagt tctatctgca
      181 acctcaaaac agtgttttga gcaacctgcg gctagtttcc tagtttgctc tttgattttc
      241 attgagtatt attaagacaa tatgaggaga aagatgaaag aaaaaggatt ttaggagggg
      301 gcgcaggcag ccatgccaac ggctcttggc tatgtcagta tcggcctggc ctgtggaatt
      361 atcggtgcgc cctatgtgac acctgttgag atgggcttga tgagtctctt tgtttatgct
      421 gggagtgccc agtttgccat gttggcactg attgtggttc aagctcctgt ggcagctatt
      481 gctatgacgg tttttctaat caatttgcgt ctctttttgt tgagcttaca cgcatcgact
      541 tatttccgtc ataccagtct ttggcacaat atcggtatgt ctagtatctt gacagatgag
      601 acctatggcg ttttgatggg cgaattagcc catacagata aggtcaatcc gatgtggatg
      661 cacgggaaca atctcaacag ctatgtggct tggtttgtgg gaacagtggt cggaacggct
      721 ctaggtgacc tgttgccaaa tccagaaatc tttggtttag attttgccct ggttgggatg
      781 tttatcggga tttttgcttc gcaattccag atgatgcaaa gacggatttc tgtccgcaat
      841 ctgctcatta tcttagcagt tgttgcagtg tccttctttt tacttttgac agtggtgtct
      901 cagtcactag ctgttctgtt tgcgacgcta cttggttgta gcatggggat ggttttagat
      961 ggtcagtaag tatcttttat tagcagttat tttctctggt cttgtgacct ggattccccg
     1021 tatgattccc ttcatcttgg tcaagtataa gggcctgcct acgattgttg agcgtttttt
     1081 gaagtttttg cctgtttcca ttatctttgc cttgattctt tcaagagtag tgacaggtaa
     1141 ggttgggaac cttcctcaaa tcaaatgact ggacttctta gcagtctttc caacagcttg
     1201 ggtagctttt cgctaccgca atctagtcgg tacggttctc tttggagtag tcttgattgc
     1261 cgtcttgcgt ttggtctttt aaataagtca taaagaaaac ttatcacaga gatagatatc
     1321 atataatggc gtaattactt tttttctgat aagattataa ggtattctat tttggaggaa
     1381 atgacatgaa aaaaatcgtt aaatactcat ctcttgcagc ccttgctctt gttgctgcag
     1441 gtgtgcttgc ggcttgctca ggtggtgcta agaaagaagg acaagcagct agcaagaaag
     1501 aaatcatcgt tgcaaccaat ggatcaccaa ggccatttat ctatgaagaa aatggcgaat
     1561 tgactggtta cgagattgaa gtcgttcgcg ctatctttaa agattctgac aaatatgatg
     1621 tcaagtttga aaagacagaa tggtcaggtg tctttgctgg tcttgacgct gaccgttaca
     1681 atatggctgt caacaatctt agctacacta aagaacgtgc ggagaaatac ctctatgccg
     1741 caccaattgc ccaaaatcct aatgtccttg tcgtgaagaa agatgactct agtatcaagt
     1801 ctctcgatga tatcggtgga aaatcgacgg aagtcgttca agccactaca tcagctaagc
     1861 agttagaagc atacaacgct gaacacacgg acaacccaac tatccttaac tatactaagg
     1921 cagatttcca acaaatcatg gtacgtttga gcgatggaca atttgactat aagatttttg
     1981 ataaaatcgg tgttgaaaca gtgatcaaga accaaggttt ggacaacttg aaagttatcg
     2041 aacttccaag cgaccaacaa ccgtacgttt acccacttct tgctcagggt caagatgagt
     2101 tgaaatcgtt tgtagacaaa cgcatcaaag aactttataa agatggaact cttgaaaaat
     2161 tgtctaaaca attcttcgga gacacttatc taccggcaga agctgatatt aaataatttc
     2221 ttgaaatcat ccattctgag taagatgggt ggtttctcag tttttaggga tatagtttta
     2281 aactatatat ctgactatct aataacaatt tgtgaaatca aacaaattgt gagatactag
     2341 tacggtatta tttttaagga gaaagaatca tgaaaatcaa aaaatggctt ggtctagcag
     2401 cccttgctac agtcgcaggt ttggctcttg cagcttgcgg aaactcagaa aagaaagcag
     2461 acaatgcaac aactatcaaa atcgcaactg ttaaccgtag cggttctgaa gaaaaacgtt
     2521 gggacaaaat ccaagaattg gttaaaaaag acggaattac cttggaattt acagagttca
     2581 cagactactc acaaccaaac aaagcaactg ctgatggcga agtagatttg aacgctttcc
     2641 aacactataa cttcttgaat aactggaaca aagaaaacgg aaaagatctt gtagcgattg
     2701 cagatactta catctctcca atccgccttt actcaggttt gaatggaagt gccaacaagt
     2761 acactaaagt agaagacatc ccagcaaacg gagaaatcgc tgtaccaaat gacgctacaa
     2821 acgaaagccg tgcgctttac ttgcttcaat cagctggctt gattaaattg gatgtttctg
     2881 gaactgctct tgcaacagtt gccaacatca aagaaaatcc aaagaacttg aaaatcactg
     2941 aattggacgc tagccaaaca gctcgttcat tgtcatcagt tgacgctgcc gttgtaaaca
     3001 ataccttcgt tacagaagca aaattggact acaagaaagc acttttcaaa gaacaagctg
     3061 atgaaaactc aaaacaatgg tacaacatca ttgttgcgaa aaaagattgg gaaacatcac
     3121 ctaaggctga tgctatcaag aaagtaatcg cagcttacca cacagatgac gtgaaaaaag
     3181 ttatcgaaga atcatcagat ggtttggatc aaccagtttg gtaataagaa acagggaggt
     3241 gggagagaaa attccacctc ttgcttttgt atagagtata gattgtaaag aagactattc
     3301 gttcatagaa aggtagagag aatatggttt ttcctagcga acaagaacag attgaaaaat
     3361 ttgaaaagga tcatgtagtc caacattatt ttgaggtttt gcgtaccttg atttctaaga
     3421 aatcagtctt tgcccagcag gttggactca aggaagtcgc aaattatctg ggtgagattt
     3481 tcaagcgtgt tggagctgaa gtggagattg atgagagcta tacagcgccc tttgtcatgg
     3541 cacatttcaa gagttcgcgt ccagatgcca agaccttgat tttctataac cactatgaca
     3601 ctgtgccagc ggatggggat caggtctgga cagaggatcc ttttacgctt tcggtccgca
     3661 atggcttcat gtatgggcgt ggggttgatg acgacaaggg tcatatcaca gctcgcttga
     3721 gtgctttgag aaaatatatg cagcaccatg atgatttacc tgtcaatatc agctttatca
     3781 tggagggagc ggaggaatcg gcttcaacag acctagataa gtatttggaa aagcatgcag
     3841 acaaactccg tggggcggat ttgttggtct gggaacaagg gaccaaaaat gccttggagc
     3901 agctggaaat ttctggtggc aataagggga ttgtgacctt tgatgccaag gtaaaaagcg
     3961 ctgatgtgga tatccactcg agttatggtg gtgttgtgga atcagctcct tggtatctcc
     4021 tccaagcctt acagtctctt cgtgctgcgg atggccgtat cttggttgaa ggcttgtacg
     4081 aagaagtaca agagcccaat gaacgagaaa tggccttgct agaaacttat ggtcaacgaa
     4141 acccagagga agttagtcgg atttatggat tggagttgcc tctcttacag gaggagcgga
     4201 tggcctttct aaaacgtttc tttttcgagc cagcgcttaa tatcgaagga atccagtctg
     4261 gttatcaagg tcagggtgtt aagactattt tacctgcaga agccagtgcc aagctagagg
     4321 ttcgtctggt tccgggccta gaaccgcatg atgttctgga aaaaattcgg aaacagctag
     4381 acaaaaatgg ctttgataag gtagaattat actatacctt gggagagatg agctatcgaa
     4441 gcgatatgag cgcaccagcc attctcaatg tgatcgagtt ggccaagaaa ttctatccac
     4501 agggcgtttc agtcttgccg acgacagcgg ggacaggacc tatgcatacg gtctttgatg
     4561 ccctagaggt accaatggtt gcattcggtc taggaaatgc caatagccga gaccacggtg
     4621 gagatgaaaa tgtgcgaatc gctgattatc acacccatat cgaattagta gaggagctga
     4681 ttagaagcta tgagtagaga tattatcaag ttagatcaga tcgatgtgac ttttcaccaa
     4741 aagaagagaa ccattacagc ggttaaggat gtgaccattc acatccaaga aggggatatc
     4801 tacggaatcg ttggatattc tggagcagga aaatcaaccc ttgtacgggt gattaatctc
     4861 ttgcaaaaac catctgcagg gaaaattacc attgacgacg atgtgatttt tgacggcaag
     4921 gtgaccttga cggcagagca gttgcgtcgt aaacgtcaag atatcggaat gattttccag
     4981 cattttaacc tgatgagcca aaagacagca gaggagaatg tagcctttgc ccttaaacac
     5041 tctgaactca gcaaggaaga aaagaaggct aaagtagcta agttgttgga cttggttggt
     5101 ttggcagatc gtgctgaaaa ctacccttca caactatctg gagggcaaaa acagcgtgtg
     5161 gcaattgcgc gtgccttggc caatgatcca aaaatcttga tttcagacga gtcaacttct
     5221 gcccttgatc cgaagacaac caagcagatt ttggccttgt tgcaagattt gaaccaaaaa
     5281 ttaggcttga ctgttgtctt gattacgcat gaaatgcaga ttgtcaaaga cattgccaac
     5341 cgtgtggcgg ttatgcagga tgggcatttg attgaagagg gtagtgtgct tgaaatcttc
     5401 tcagacccta aacaaccttt gactcaagac tttatctcaa cagctacagg tattgacgaa
     5461 gccatggtca aaatcgagaa gcaagaaatc gtggaacact tgtctgaaaa cagtctcttg
     5521 gtgcaactca agtacgctgg atcttcaaca gacgagccac ttttgaatga attgtacaag
     5581 cattatcaag taatggctaa tattctctat gggaatatcg aaatcctcga tggtactcct
     5641 gttggagaat tggtggtggt cttgtcaggt gaaaaagcag cgttggcagg tgcccaagaa
     5701 gccattcgtc aagcaggcgt acagttaaaa gtattgaagg gaggacagta agatggaatc
     5761 attgattcaa acctatttgc caaatgtcta taagatgggt tggtctggtc aggcaggctg
     5821 gggaacagct atctacctaa ccctctatat gacagttctt tccttcatta tcggaggctt
     5881 cttggggcta gtggcaggtc tctttctcgt cttgacagcg ccaggtggtg tcttggagaa
     5941 taaagtcgta ttctggattt tagacaaaat tacctcaatt tttcgtgcga ttccctttat
     6001 catcctcttg gcaatcttgt caccactttc tcacttgatt gttaaaacaa gtatcgggcc
     6061 aaatgcagcc cttgtcccac tttcttttgc agtctttgcc ttctttgccc gtcaggtgca
     6121 ggttgtcttg gctgaattgg atggcggtgt cattgaggcg gctcaagcga gcggagcgac
     6181 tttctgggac atcgtgggtg tttacctatc agaaggtctt ccagatttga tccgtgtgac
     6241 gactgtgacc ttgatttccc ttgttgggga aacagctatg gccggtgcgg ttggagctgg
     6301 tggtatcggt aacgtagcca tcgcttatgg atttaaccgc tacaatcacg atgtgaccat
     6361 cttggcaacc attattatca ttttgattat ctttgcaatc caattcttgg gagatttctt
     6421 gactaagaaa ttgagtcata aataaaaaga gccgcgtggc tctttttaac tgatcagatt
     6481 ttctgtgcaa attttttact caaggcttgt ccaatcaagg cacccactag ggctccgatg
     6541 acaatacttg cgataaatag aaggacagtt ccagggtttg gagcgaccat gatacggtcg
     6601 atatattctt gggattttcc tcttgccaga agagtagcca tataggcttt gggcgcaatc
     6661 cacataagca agattggtcc tgttgtacta aaggcgaaaa taatgaaaga aaggaagttc
     6721 tttgttttgt ccttgtattt tcctaaatga gctacttcat ctgctaggag gccacagata
     6781 attccaggaa ggaaggaacc agcaccgtgt ttagttccca agaaaaagag ggcaatgaca
     6841 aggccgatag tggtaatggc tccaaagcgc ggaacttttg cgactaggat catatagacg
     6901 ctaccgccga caagggcagt aaaggcaggc gcataaaaca tgtttccagt ttggtcaaag
     6961 agattgccca aaaggacacc aatccccatg cagaggaagt aaagaactgc aaaaagcagt
     7021 gtagttaaga tagatttctt catgaattgt ctcctgataa ttttttcaca attctcattg
     7081 taccacggtt tggtgggatt gtaaatggat actagaagtt tttgaaaacg cttgaaatat
     7141 gatataatgg tttcatagtt atttttaggg gaatatcatg ggtagatttt tagactttgt
     7201 ctttaatcgt ttctttttag ggatgattgc gacagccttc ttttggctat taactttagc
     7261 aggagggatt atccttggtc tagcgccggc tagtgccacc ttgatgagct tatatgcaga
     7321 acatggttat agctttcggg aatacagttt gaaggaggct tggtctcttt acaagcaaaa
     7381 ttttgtctca agcaacctga ttttctatag ctttttaggt gtgggtctag ttttgaccta
     7441 tggtttgtat ctcttggtgc aattgcctca tcagaccatt gttcatttga ttgcgaccct
     7501 tttgaatgtc ctagtagttg ccctgatctt tttggcttat acagtatctt taaaattaca
     7561 agtttatttt gccttgtcct atcgaaatag tctcaaatta tccttgattg gcatctttat
     7621 gagtctagca gctgtggcca agattctcct tgggactgtg ctacttgtag caattggtta
     7681 ctatatgcct gccctgctat tttttgtagg aattgggatg tggcatttct ttatcagtga
     7741 tatgttggaa cctgtctatg aaatcatcca tgaaaaattg gcgacaaaat agaatgaagc
     7801 agttttggct acatacgctt ctaagaacct atagttcagt gatgatcatt atcattgcga
     7861 gttttgcaat cttactctct tacgctgact gggattcacg tgaaaaggaa gcccagagag
     7921 tagcccagcg tgtaactgct agaacagtga gtgaaattga atattaccat agagagtcaa
     7981 cccagatagc tcaggcttta gttgaaaacc aagctcgtat tgagggaatc tataaatact
     8041 ttagtcttag catgccagac tatttttact ggcaattaga gcggaaagct tcgccttata
     8101 tatcagtctc tctgtatgaa aatgttgatg acctctatgt tcgaaatgat tttgtaactg
     8161 gagtggccat tgcttttcaa gattacaagg aagtctatgt ttctactaaa gacaaacgta
     8221 gtggagaaaa aatcagggct gaggatttca aaccagcagg aaatagtttt gccattccgg
     8281 tgtcagatcc agtgtcagat caagatttag gagtgattta catctccttg gatcctgctg
     8341 ttttatacca tgccattgat aatactagag gtcatactcc gatggcagta acagtgacct
     8401 caccttttga tacggagatt tttcatattg gtgagacagt tgataaggag agtgaaaatt
     8461 ggctagttgg cttaacttct catggatatc aggttcaggt ggcagttcct aaaaactttg
     8521 ttttacaagg aacagtgact agctctgctt tgattgtggg tttgagcctt ctctttattg
     8581 tcattcttta tctgactttg aggcagactt ttgctaatta ccaaaagcag gtagtggatt
     8641 tagtagaatc cattcaagtc attgctcaag gcgaagaggg gcgtcggatt gacatttccg
     8701 agaaagatca ggaattactc ctaatcgcgg agacgaccaa tgatatgttg gatcgattgg
     8761 aaaagaatat ccatgatatt taccagttag agcttagtca aaaagatgcc aatatgcgag
     8821 ccttgcaggc gcaaatcaat cctcatttta tgtataatac gctggagttc ttgcgcatgt
     8881 atgcagttat gcagagtcaa gatgagttgg cagatatcat ttatgaattc agtagtctct
     8941 tgcgtaacaa tatttccgac gaaagagaga ccctcctcaa acaggaatta gaattttgcc
     9001 gtaaatacag ctatctctgc atggttcgct atcccaagtc cattgcctat ggtttcaaga
     9061 tagatccaga gttagagaat atgaagattc ccaagtttac cttgcaaccg ctggtagaaa
     9121 actatttcgc gcatggtgtt gaccacaggc ggacagataa tgtgattagc atcaaggctc
     9181 ttaaacagga tggttttgtg gaaattttgg tggtcgataa tggtagagga atgtcggctg
     9241 aaaagttggc aaatatccga gaaaaattaa gtcagagata ttttgaacac caagccagct
     9301 acagtgatca aaggcagtct atcgggattg tcaatgtaca cgagcgtttt gtgctctatt
     9361 ttggagaccg ctatgccatt actatagagt ctgcagagca agccggtgtt cagtatcgta
     9421 ttacaattca agatgagtag aaagggagaa aatgtataaa gtattattag tagatgatga
     9481 gtacatggtg acagaaggtc tgaagcgttt gattcccttt gataagtggg atatggaggt
     9541 tgtcgcaaca gccagtcatg ccgatgaagc tctagaatat gttcaggaaa atcctgtcga
     9601 tgtcatcatt tccgatgtca atatgccaga caaaacaggg cttgatatga ttcgggagat
     9661 gaaagagatc ttaccagatg ctgcctatat cctgctctca ggttatcagg agtttgatta
     9721 tgtaaaaaga gcaatgaacc ttagtgtggt ggactatttg gtcaagcctg ttgataaggt
     9781 agagctggga aatctgctgg agaagattgc aggtcagctc ggcgagagag ggaagaaaag
     9841 tcagactctt agtcaagaat tagacgaggc tggatttgtt agttatttag gggataagga
     9901 gaattggtgg ataggtctat ccaaggaaaa acaaggttcc ttcaccattc cctactatgt
     9961 cttgggccaa gactggcaga ttttcatttc tggccaacct ctagatggtt tagtcgttac
    10021 accttttgaa gctccttatc aagaacactt tgaacgctgg aagctgaatg ctgagaaaac
    10081 cctcttttac ggttctgtaa atttgcagca gtctgagagt ctctttgcct attacgaacc
    10141 gatttatagg gttatcattc agggaaatct caatcaaatc gtagaagagt taaatctctt
    10201 ggagaaggta gttcttgaaa atacgccgcg tgttccgatt actaaacagc tttttattca
    10261 gtttgtcatg gatgttttcc atttatttga acatctcaaa gctgatgacc tgacagacat
    10321 cgtcaaaacc attcatgcta ttcaatcctt cgatgaattg gtttcttata tcaaggaaac
    10381 tctgatcagc tttttcggtc aataccgtat gaatgaaaat gtggtcagtg tgctggaagt
    10441 cattggtcgt gattaccaaa aagagctttc cctcaaggat atcagtaagg ctctctttat
    10501 caatcctgtt tatctagggc agttgattaa gcgtgaaacc gattcgactt ttgcagagtt
    10561 actaaacaaa caacgtatta aggctgccca acaacttttg ctttcaacta gtgacagcat
    10621 cgaagatatt tgttatgctg ttggttacag taaccttgga tatttctata aagttttccg
    10681 aaaattgtgc ggaaaatcgc caaaagccta ccgaaaacag gtagaaacta tactataaga
    10741 tttgtattcc tttacaaaag gtgctataat attatcagga ggttctatca tgaaaaagaa
    10801 accaatttat ctatgggtct tgctaatctt gtctgccctt atttcagttc cgtctctgtt
    10861 tggaattgtg agtcccttgc ctagtaaaga agcccttcgt gctgctcaga aacaagttgc
    10921 aggggtcaat gctcagcaat tagaagacca gcttaattat acctatagag tagcagaagc
    10981 atctcattct atttttaatg ttgctttgat tgtgctatct actattttag tggtcgtagc
    11041 gattgtcttc cttgttcgta aaaatttgca atatgcaaac tatacttacg ttggctatgt
    11101 tttgttagct attattggtt cgatttatgg ctatgttggt ttacaagacg ctgtgcagtt
    11161 ggttcaagat gagagcatgc gtttgacagt gagtattggt tcaaaagctg ttagcatttt
    11221 ctatatcgtc atcaatgttc tcttcctagc ccttgtcttc tataagatgt ggcgacaaca
    11281 gaaagccttg gcagaagaag aggaaacaga agagcttacc taagtattga caaaaaagaa
    11341 ctatcaagat acaatcttgg tagttgtttt ttgattatag ataaaatcat ggaggtacat
    11401 atgaagacaa tttctttagt ttatattagt ttgagtggca acactgaaag ttttgtgacg
    11461 cgcttgaaag actatctctt gtcccagtac aaagggattg aggttcaaaa gattcatatc
    11521 aaggatttgg tcaaggaagg caaaaatttc tatgaaatgg accatcccta tgtcgctttt
    11581 ttgccgacct atctcgaagg tgggaatggc gtggataacg gagatgttga gattttgacg
    11641 acaccggtgg gagattttat tgcctatggt aacaatgcta gtaagtgttt tggtgtggtt
    11701 gggtcaggaa atcgtaactt taataaccaa tactgcctaa cagccaagca atacagtcaa
    11761 cgctttggct tccctgtatt ggctgacttt gaaatgcgag gcatgctgga agatatcaaa
    11821 catgtcgcag ctattatcgc agatttgtat gagttggaaa aggagaatta atactcaatg
    11881 aaaatcaaag agcaaactag gaagctagtc gcaggttgct caaagcacag ctttgaggtt
    11941 gcagatagag ctgacgtggt ttgaagagat tctcgaatag tataaagttc ttataagatg
    12001 tggttgaagt tgttcaactg ttttttgagt ataaacagtc tttaagaacg taaaaaagca
    12061 taagttcagg tcttcaaaac cttgttctta tgctttttat catagaaata tttgctgaat
    12121 tttctcttga aatgaaacct tatcttcatc tcgcgcttgt cccttaccct gcttttccga
    12181 ttgcgagtgc gtagataaag aagatggcga agccaaagag gaaaatcaat cccgcaatgg
    12241 caaggtgttt gagccaagaa ggaagatttt tgttttcacg tgttaccaag gcgtaattca
    12301 aaagagcaaa gaaaggtgtt gtcaggaaag agccaatcat ggcaaagcgg agcatggttg
    12361 aaacctgacc agcgaagaac ttgataatga cgataccgat gatagcagtg atggtcatcc
    12421 agatgttcaa agatttacga ttgtcctctt tttgactgat tagcagtcgg agagattcct
    12481 gattaacgcg agaatagcca tcgataacag ttataactgt tccaaagata cagaggaagg
    12541 caataaaggt aatcaagtaa cgggaccatt cgccaagaac agaggcatac atgcccacga
    12601 attgagagat gtatttggct gaagcagctt caaccgcctg ccctgtagga tactgaatca
    12661 gtgctcccag tgccacaaag aagacggcta ggatagctgt tccaatataa ccagtgttaa
    12721 agtcaaacag agcgtcttct gtgttaaagt tgacggtctt tctcttttca gctgaccaaa
    12781 gtgaattgat ggctgaaatt tcaataggag ccggcatcca tcctaggagg gagacgatga
    12841 agggcagagc tgccatttgc caaggtgtct tctcgacaaa atcagaactg tattctggat
    12901 gcttgaccgc cgcaatgata actgcaagaa cagtcgcaat ggttaaggca gacataatcc
    12961 atttgaccat gccgtctaaa agtttgtagc ctccaaagag tagcatagcc caaatgattg
    13021 caacgagaat gagggaccac tgagtaatgc taagtccaat cattgggaag gcactggcga
    13081 tgatagctga gcacagaatg gcaacaccag ccgtgttgac catagccgaa aagacattga
    13141 gaataaagaa aatccagaga tagagttttc ctttttcggc ataaccttca accaaagtct
    13201 ttccagtatc agctgtgtat tcagcaccaa aacggaaaaa tggatattta aagacattgg
    13261 ctaagatgac caagagaagt agagaccaac cgtaagaacc gccagcttga gttgaggata
    13321 caatgtggga acctccaaca gcggcagttg ccattaggat tccaggtccc attgctttta
    13381 gcttgcttgc ccaggttgat tgatttaagg aaatagcttg cgacatgata aatactcctt
    13441 tttgaatttt cagaataatc tgtatatgca atctatattc tacaaaaaat tttggaaaaa
    13501 tgcaagaata ttttgaaaaa agatagaaaa tttacaaaga aaatctgaaa taactgttat
    13561 gatatagcag attgaaacta gaatagtaca cttttgcttc taaaacattg ttagaaatcg
    13621 atttgactgt cctgattgat ttatcctgtt cttatttcat ttcactatag taaggtgcag
    13681 caggataaag caaaaccgag aagtttcttt ctcggttttg cttattatga ggttcctatt
    13741 ttctatgctc caagagttga ttgatgtgat ctactttttt agcctctctt ttatagagaa
    13801 tagcccagat gatgagatag acaatcgcaa actcgataat gagctggagg tagaaaatcc
    13861 agtggaaggg gaaccaacca gcaagagttg ctagtgggac aaagccagcc agcataagga
    13921 agaaatgagt cagagtggca cgaagcatgc tccagtcacg gctgaataag cggttgccaa
    13981 agttgaagag cataccgatg gctgcccaga taagtgtgca gtagagcaag accagggcac
    14041 cgtgaacctg atgctgatcc atcacttggc cgatgagaga gtagggattt agtggtgcgt
    14101 aggtatttgg tgcataaatg agtgaaaaga ggatggagag gatgaggccg ataagaacac
    14161 cggcagctgc atcgtgaaat acttgttttt tcatagttct aatttctcct tgatggtttt
    14221 tagataacgg cgtgaagagt aggtgaagct ttcgtttttc aagaaaattt ctaccagacc
    14281 gtttggcgtg agcttgaggt gagagatgga atcgatattg atgatttctg attgggaaat
    14341 ttggataaaa ttgcttggca agagtttaag aacctgatag agtcgcaaat caatgctgta
    14401 ggtctgactc gcggtttctg ctagaacctt ccgattctcg atatagaagc gctgaatctt
    14461 gccaatctca actagataga cctgatcatc gatttttcct ttgatttttt ctctttggtc
    14521 cagattttct gcgaactcga tgactttctg gactttttcg gtttcttgag gtgcttggac
    14581 aatcagcttt tcctcctcgt aagtctcact aatctgtagt tctactttca taaatttctc
    14641 tccttgtttt tcatacaaaa ttaatctcac ttcttgtaca cctttattaa tactcttcaa
    14701 aaatctcttc aaaccacgtc agcgtcgtct tgccgtagga tagttgttga ctttgtcagt
    14761 tttatctaca acctcaaaac agtgttttga gctgtctgcg tctagctttc tagtttgctc
    14821 tttgattttt attgagtata ccactatttt actccctctg gcaagggact ttgtctatgt
    14881 ggagggattg ggctcctatg tggtggagtt tttctgttct ttctgaaata tggtataata
    14941 gcactaatta atttctagga aaatagatac agaaaggggc tgaaagatgt ctcatattat
    15001 tgaattgcca gagatgctgg caaaccaaat cgcagctggg gaggtcattg aacgtcctgc
    15061 cagtgtggtt aaagagttgg tagaaaatgc cattgacgcg ggctctagtc agattatcat
    15121 tgagattgag gaagctggtc tcaagaaggt tcaaatcacg gataacggtc atggaattgc
    15181 ccacgatgag gtggagttgg ccctgcgtcg ccatgcgacc agtaagataa aaaatcaagc
    15241 agatctcttt cggattcgga cgcttggttt tcgtggtgaa gccttgcctt ctattgcgtc
    15301 tgttagtgtc ttgactctgt taacggcggt ggatggtgct agtcatggaa ccaagttagt
    15361 cgcgcgtggg ggtgaagttg aggaagtcat cccagcgact agtcctgtgg gaaccaaggt
    15421 ttgtgtggag gatctctttt tcaacacgcc tgcccgtctc aagtatatga agagccagca
    15481 agcggagttg tctcatatca ttgatattgt caaccgtctg ggcttggccc atcctgagat
    15541 ttcttttagc ttgattagtg atggcaagga aatgacgcgg acagcaggga ctggtcaatt
    15601 gcgccaagca atcgcaggga tttacggttt ggtcagtgcc aagaagatga ttgaaattga
    15661 gaactctgac ctagatttcg aaatttcagg ttttgtgtct ttacctgagt tgactcgggc
    15721 taaccgcaat tatatcagcc tcttcatcaa tggccgttat atcaagaact tcctgctcaa
    15781 tcgtgctatt ttggatggtt tcggaagcaa gcttatggtt ggacgttttc cactggctgt
    15841 cattcacatc catatcgacc cttatctagc ggatgtcaat gtgcatccaa ctaagcaaga
    15901 ggtgcggatt tccaaggaaa aagaactgat gaccctggtt tcagaagcta ttgcaaatag
    15961 tctcaaggaa caaaccttga ttccagatgc cttggaaaat cttgccaaat cgaccgtgcg
    16021 caatcgtgag aaggtggagc aaactattct cccactcaaa gaaaatacgc tctactatga
    16081 gaaaactgag ccgtcaagac ctagtcaaac tgaagtagct gattatcagg tagaattgac
    16141 tgatgaaggg caggatttga ccctgtttgc caaggaaacc ttggaccgat tgaccaagcc
    16201 agcaaaactg cattttgcag agagaaagcc tgctaactac gaccagctag accatccaga
    16261 gttagatctt gctagcatcg ataaggctta tgacaaactg gagcgagaag aagcatccag
    16321 cttcccagag ttggagtttt tcggacaaat gcacgggact tatctctttg cccaagggcg
    16381 agatggactt tacatcatag atcagcacgc tgctcaggaa cgggtcaagt acgaggagta
    16441 ccgtgaaagc attggcaatg ttgaccagag tcagcagcaa ctcctagtgc cctatatctt
    16501 tgaatttcct gcggatgatg ccctgcgtct caaggaaaga atgcctctct tagaggaagt
    16561 gggcgtcttt ctagcagagt acggagaaaa tcaatttatt ctacgtgaac atcctatttg
    16621 gatggcagaa gaagagattg aatcaggcat ctatgagatg tgcgacatgc tccttttgac
    16681 caaggaagtt tctatcaaga aataccgagc agagctggct atcatgatgt cttgcaagcg
    16741 atctatcaag gccaatcatc gtattgatga tcattcagct agacaactcc tctatcagct
    16801 ttctcaatgt gacaatccct ataactgtcc tcacggacgt cctgttttgg tgcattttac
    16861 caagtcggat atggaaaaga tgttccgacg tattcaggaa aatcacacca gtctccgtga
    16921 gttggggaaa tattaaaagt ataaaaaagt ctgggaaaaa ttttcaaaat cagaaaaacg
    16981 cataaaatca ggtgttcaaa aaccttgatt ttatgcgttt tatcatggaa atagttactt
    17041 catgttttcc taattctttt cgaaactctt tttaaaccac gtcagtttta tcagtaatct
    17101 caaaacagtg ttttgagcta attttgccag ttttgtctgt aacatcgaag ttgtgtttta
    17161 ccactctgcg actggtttcc tagtttgctc tatgattttc acagagtatt aaattgcgat
    17221 tttgccaagt ttctttattc gtctaaaagt agagtctgtt ctatgcgtct aatgtacgaa
    17281 ttaggttgac catttcaata gctccttgtg cacactcaga acccttattt cctgctttgg
    17341 taccagctcg ttctatggct tgttcaattg tatctgtcgt tagcacacca aacataacag
    17401 gaatttcgct atttaaactg atttgggcga ttcccttaga tacctcgcta catacataat
    17461 cataatgact tgtattccct ctaatgacag ctcccaagca gataattgca tcatattttt
    17521 tactttttgc catttttgat gcaatcagtg gtatttcaaa agctcctgga acccaggcta
    17581 cctctatatc tttctcgttt acattctctc ttttgagatt atctagtgct ccagataata
    17641 attttgaagt tataaattca ttaaatctcg ctacaacaat acctatttta atattgtttg
    17701 ctactaaatt accttcataa gtgttcattt atttttcctc catatttaaa atgtgaccca
    17761 ttcgattttt ctttgtttct aaataaaaac tatcgtaagg attggcttct atttcgattg
    17821 atattctact ggaaatggta attccatatt tttctaactg ttcaaccttg tcaggattat
    17881 ttgtcagtaa atgaagtgac tgaagtccca gatctttaag catttgtgct ccaatatgat
    17941 attctcttaa atcaccttca aagcctaatg caagattggc atcaagcgta tccatgcctt
    18001 gatcttgtaa atgataggct tttaatttat tgataagtcc aattcctcgt ccctcctgtc
    18061 gcaagtaaag taagacaccc gaaccattct caacaatcat tttcatagct ttatcgaatt
    18121 gctgtccaca atcgcaacgt aaagagccta aaacatctcc tgttaaacat tcggagtgga
    18181 cccgacataa tacattggct tcatcctcta tgtttcccat aataagagca agatgatgtt
    18241 ccccatttag tttatctata tagctaattg ctttgaaatt accgtatcta gtaggcatat
    18301 tgacagttga aactcgttct atcagctgat catatacttt tctatattct tgtaattctt
    18361 tgatggtaat tagtggaatg ttgtgtttct tcgagaactg aattaaatca tctgttctca
    18421 tcattttgcc atcatgattc attatttcac aacataggcc acactctttt agtccagcta
    18481 attttaataa atcaacagtt gcttctgtgt gtccatttct ttctagaaca ccaccttttt
    18541 ttgcaattaa aggaaacatg tgtcctggcc tgcgaaaatc agagggtgtt atatcttcag
    18601 ctacacacat acgtgcggtc agtcctcttt cctcggcaga aatacctgtg gtcgtttctt
    18661 tataatcaat tgaaactgta aaagcagtct tatgattatc tgtattgttt tcaaccatag
    18721 gtgaaagcat taattgatta gctaaacttt cgctcatagg catacaaatt aatcctttgg
    18781 cataagtagc cataaaatta acattttctg ttgtagctgc ttgtgcagaa caaattaagt
    18841 ctccttcatt ttctctatcc ttgtcgtcta taacaagaac aagtcgtccc ttctgcaatg
    18901 cttctaatgt ttcttgtatt tttcgatatt ccattgactg attatccttt ctgctaaaat
    18961 ccattttgat ataatagttc cttagatatt tctgattttg gagagttatc catcagtttt
    19021 tgcacatatt tacctaagat atcattttca agatttactg tactcccgac ttgtttactt
    19081 ttaagaatgg tttgtttcaa ggtatgaggg ataacagata ctgaaaagtt tactttggag
    19141 actttagcga cagtcagact aatgccgtca attgtaatag atcctttttc aactattaaa
    19201 tctaaaattt ctttttgtgt gttgatttga taccatacag cattatcatc tttttttatt
    19261 gacgagattt ttcctgtacc atcaatgtgt cctgtaacga cgtgaccccc aagtcgaccg
    19321 ttgatagata aggctctttc tagattcacc tcacttccat gttttaatag agtaagagct
    19381 gttcgactcc atgtttcatt cattacatca actgtaaagg attgatgatt gaaatgagta
    19441 actgtaagac agataccatt tactgctata ctatcgccta aatggatatc cgttaatatt
    19501 tttgaggctt taattgatag tttacaatta cgagagtctt tctgtattct ttcaactttt
    19561 ccgatttctt caattattcc tgtgaacatg gataaatcac ttcactttct atgagatagt
    19621 catttcctat ttgagaaaaa gcataaggtt tcaatctaat agcgtcattt ggcaaagaaa
    19681 tgccttcacc tccgacagga aacttggcac tgcctccaaa aatttttggt gcaatatata
    19741 ttttcagctc atcaacaatt tgttgttcca aagcactcca attcattaga ctgccccctt
    19801 ctagaactag gctatcaatc tgcatgtttc ctagatgttg cattaaactc gataagtcta
    19861 tatgattgcc ttttttcttt atggaaagta tttcacagcc atgattttga tatagcttca
    19921 ttttattttt gtcttcagag gaagtggcaa tgtaagtttt aatatcattt gctgttttta
    19981 cgattttaga ggtaagagga gttcgtaaat gtgtatcgca tatgatacgg ataggatttt
    20041 tcccttcctc caatctacat gtcagcaaag gatcgtcttg aataacagta ttgactccca
    20101 ccataattgc actaacatgg tgtcgtaact gatgcacatg ctttcttgct tcttcttcag
    20161 taatccattt ggattgattt gttttagtgg ctatttttcc atccattgac attgcatatt
    20221 tcataaaaac atagggtaca tgctgggtaa tatactttct aaaacttttt attaagttaa
    20281 gacactcatt ttctaaaatt ccaacagtaa cttgaagatt attttcctca agtatcttta
    20341 ctccttttcc agatacaata ggattacagt ctaggcttcc aatgactact cttgtaatac
    20401 cactatcgat tatagcatct atacagggag gtgttttccc gaagtgacaa cagggttcaa
    20461 gtgttacata aagcgtcgct ccgacagggg attctctaca gtttttaaga gcatttctct
    20521 cagcatgtgg gccaccaaaa aactcatgat aaccttgtcc gatgatgtga ttatctttta
    20581 caataactgc gccgaccata ggattgggat tgacgtaacc agcccctttt tgtgccagtt
    20641 ttattgctaa tttcatatat tttgaatcgc tcatctcact acctccaaaa aaatatacct
    20701 tgaatagggg actactcaag gcatacaaaa gaaaacttat gcgattaaca aaaatactct
    20761 gaaatgacaa gtaatcattt cagagcacgc aaaaagcaca aatatacttt tatcttcttt
    20821 catccagact atactgtcgg ctttggaatt tcaccaaatc atgcctttcg gctcgtgggc
    20881 tataccaccg gtagggaatt acaccctgcc ctgaagatag ttattcaatt acagatgatt
    20941 atagtactta attttgaata tgtcaacaga taaataccga ttgtttttga tatactgtat
    21001 ttgtgataat cgattctcgc tcctcggata aagaaaatat gatatactag ataaacgaaa
    21061 taagagagaa ggaatactat gtacgcatat ttaaaaggaa tcattaccaa aattactgcc
    21121 aaatacattg ttcttgaaac caatggtatt ggttatatcc tgcatgtggc caatccttat
    21181 gcctattcag gtcaggttaa tcaggaggat cagatttatg tgcatcaggt tgtgcgtgag
    21241 gacgcccatt tgctttatgg atttcgctca gaggatgaga aaaagctctt tcttagtctg
    21301 atttcggtct ctgggattgg tcctgtatca gctcttgcta ttatcgctgc tgatgacaat
    21361 gctggcttgg ttcaagccat tgaaaccaag aacatcacct acttgaccaa gttccctaaa
    21421 attggcaaga aaacagccca gcagatggtg ctggacttgg aaggcaaggt agtagttgca
    21481 ggagatgacc ttcctgccaa ggtcgcagtg caagcaagtg ctgaaaacca agaattggaa
    21541 gaagctatgg aagccatgtt ggctctgggc tacaaggcaa cagagctcaa gaaaatcaag
    21601 aaattctttg aaggaacgac agatacagct gagaactata tcaagtcggc ccttaaaatg
    21661 ttggtcaaat aggagcagag aatgacaaaa cgttgttcgt gggtcaagat gacaaacccg
    21721 ctctacatcg cctatcatga tgaggagtgg ggtcagcccc tccatgatga ccaagcattg
    21781 tttgagttgt tgtgtatgga aacctatcag gcaggcctgt cttgggaaac ggtactcaac
    21841 aaacgccaag ctttccgaga agcctttcat agctatcaaa ttcactcagt cgcagagatg
    21901 actgagactg aattggaagc catgctggag aatccagcta tcattcgaaa tagagccaag
    21961 ctttttgcta cacgcgctaa cgcccaagtc cttctacagt tacaggcaga gtacggctct
    22021 tttgatgcct atctttggtc ttttgttgag gggaaaattg tcgttaacga tgttcctgat
    22081 tatcgccaag cgccagctaa aacaccctta tctgagaaat tagccaaaga tctcaaaaaa
    22141 cgaggcttca agttcacagg cccagtcgcc gtattgtctt ttctacaggc tgcagggcta
    22201 gttgatgacc acgagaatga ttgtgagtgg aaaggtctta aatgatgtct aacaaaaata
    22261 agggaattct gatttttgcg attctctata cagtcctctt tgtgtttgat ggcgttaaat
    22321 tgctggcttc tttaatgcca tctgtcattg caaattatct tgtttatgta gttttagctt
    22381 tatatggctc cttcttgttc aaggatagat tgatccaaca atggaaggag attagaaaga
    22441 ctaaaagaaa attcttcttt ggcgtcttaa caggatggct ctttctcatt ctgatgactg
    22501 ttgtctttga atttgtatca gagatgttga agcagtttgt gagactagat ggacaaggtc
    22561 taaatcagtc taatattcaa agtacctttc aagaacaacc actactgata gctgtttttg
    22621 cttgtgtcat tggacctctg gtagaagaat tatttttccg tcaggtctta ttgcattact
    22681 tgcaggaacg gttgccaggt ttactaagca ttattctggt aggacttgtt tttgctctga
    22741 ctcatatgca cagtttggcc ctatcagagt ggattggtgc agttggttac ttaggtggag
    22801 gccttgcctt ttctattatt tatgtgaaag aaaaagagaa tatctactat cccctacttg
    22861 ttcacatgtt aagcaacagc ctctccttaa tcattttagc tatcagtata gtaaaatgaa
    22921 atgagaacag gacaaatcga tttctaacaa tgttttagaa gtagaggtgt actattctag
    22981 tttcaatata ctgtaatatg tgatgaaaat gccagtaatg ataccgagaa aaaagctgag
    23041 aaacttttcc cagctttatt tgttatagtc aaagagaatg acttgttcct gtgcatctac
    23101 atgagcatgg accccaaagg gtacaattgc tcttggagtt gcgtggccga cattcagatt
    23161 atagacaatc gggatattgc tgtcaatgat atccaatagt gcctctttat agtcgtcatg
    23221 gaaagtttca tccataggtt ttccgaccaa gagtccattg atgaccgcga atatgccagt
    23281 gtcctttaaa gttagcaaca tctttttgaa gtcttctggc ttaggctttt cttcgcttgt
    23341 ttcgagcaag aggattttcc cttcccagtc tgacaagtca gggaaaagtt tgtatttttg
    23401 gcagagttcc gtgctatctg cgtatcgaga gttgtcaaag atatcgtaga gggattcgag
    23461 gcaaccaccg aggattttcc cctcgaactg ggcacttcct tgcaacaagt caaaacctgt
    23521 atttgtatga ctgacacgag gtgttcccag ggccgtggga ctaaaatcag ttcgttcctc
    23581 ataccaaacg tcactagggc ggatttctga aattcttccc gtctcaatca attctttaaa
    23641 gtagtgaagg ctataggcta gcatttcttt gtctaattca caaatgtctg ctaaaaagga
    23701 ttgaccataa aaagtcttga ttcctaattt atgcaacatg aggtggttca tggttgtatc
    23761 cgagaagcca agaaaaattt tttgtttgat aaccttttgg agttggtcat tttcaaaaag
    23821 ataaggtagc aagcgatagg tatcgtctcc accgatggca cataggatca tgtcgatgct
    23881 atcatcagaa aaggcatgaa tcaaatcctc tgcacgagct tcaggatggt ccttgataaa
    23941 gtctaatcct tttaacgaat ggggcaaaaa gatgggattg agtcccagat ccttgagacg
    24001 ttggataccc aagtccactt cgtgtttgac aaaatcctct ccgataatgc cactagataa
    24061 actaacaata ccaatagtag aaaccatatc tcatcctcct agaaatagat tgagcctatt
    24121 ttatcacaaa atataagaga aagctatgtg ggatttcaga agaatacttt taatactctt
    24181 cgaaaatctc ttcaaaccac gtcagtttta tttgcaacct caaagctgtg ctttgagcaa
    24241 cctgcagcta gcttcctagt ttgctctttg attttcattg agtataactc aaaaaagtaa
    24301 gtgaaaaagc aaccgcacct gcagttgctt ttatcgtttt cttattcttg atagcgtgtt
    24361 ggtcctgcac ttgcactacg gatattcaag cgtttcttga gatagaagcg aagggctagg
    24421 agggctactc caataatagc tagtggcaat ggatccagta ctgggttaag gctagctggt
    24481 aggaagcttg ttgcaaagaa gacaagcaac caaaggaaca tagaagttag gataactagt
    24541 acagatttcc agaaaggtgg acgttgactg cgatccatat ctggtccata gtattggtaa
    24601 acaaagtagt acatcaagta gaaggcaaat ccaccaacca gtccaactaa tagaagagta
    24661 atcaatccat agccgaaagc ttgatctgcc gcaaagaagg ttgtgagggc gctgacaagg
    24721 gcaaagaggc tagtgatgaa aagagctgag tccataatca tcagttttgg atcatcattt
    24781 tcttttggat gctctttttc gtactgctct ttgacagtga agctatgagc ccaatgagtt
    24841 ggtgcgccgt atagggaacg ggcagttgta cctttagatt gctcctcaag gatttgggga
    24901 atgacttcct caaaaatagt ctggatttca gcgtctgttt tcccatcttt gatgaattgt
    24961 tgggtagcaa tgtggacaaa ctcttggttt ttcttagtta atttttgtag atcaatttga
    25021 gacataggaa ctcctcttag aaccattttt tatggatgag atagagagtg agcgagacac
    25081 tcatagcaaa ggcgataaag acgattaacc agaaggcatt tggctctccg tttaggggaa
    25141 tttcattatc cttaaagttc atcccgtagg cagaaaagac catggttggg atggacatga
    25201 cgatggtcac aagggccaag gttttcatga tgttgttctg gttgttagaa atgatagagg
    25261 caaaggtctc tgtcatagaa tgcaagacgt ttccataaat atctgccatc tcgatggcct
    25321 gttgggtttc aatcagggtg tcttcaagca ggtcttcgtc ctcaaggtat ttcttgatat
    25381 tgctggttga actggtcaat ttcttaatca cgcgctcatt tgttttgagg gaggccttga
    25441 aatagacgat agttttttcc aattccatga gctcaatcaa ttcttcatta cgagttgatt
    25501 gatgcagttg actttcgatt tgttcactct tgcggtcgat tgaacgaagg gctgttaggt
    25561 aaagctctgc attgcgataa agaatttgaa agataaaacg tgaacgcatg aaggtataga
    25621 aattacgcaa tcgacggttg ataaagacat caaggacagg tagtggttcc aaacacgtag
    25681 tgataatggt ttcctcagtg atgataatac caagcgggat ggttacgtag taggtgcggt
    25741 tatttctttc ctccgtgacc ggcacgtcta cgataatcag ggtatactcg tcttcaatgg
    25801 taatacgaga catttcttcc gcatcgagcg gtgctcgaag gtcggcaata tcaatatcga
    25861 aggtgttggc gatttcgagt gattcatttt gagtcggatt gacgagattg atccaagtac
    25921 ccggttcaag cgtatcgatc tctttaaatt cagttgttgt agagagaaaa acttgtttca
    25981 tatcccttat cctttctcat ttttcagatt ttttcacact gtactattat actacaaaat
    26041 cggccttttg ggtaatagaa tctcactttt tttggtataa tggtaagcaa taatggacta
    26101 gaaagaacaa agatgcaaga taaaattgtc attcatgggg cgcgtgccca taatttaaaa
    26161 aatattgatg tggagattcc gcgagacaag ttggttgtcg tgactggctt gtcaggttca
    26221 gggaagtcca gtctggcttt tgatactctc tatgcggagg gacaacgtcg ctatgtagag
    26281 agtttgtctg cctacgctcg tcagttcttg ggaaatatgg aaaaaccaga tgtagatgcc
    26341 attgatggtc tcagcccagc tatttccatc gaccagaaaa cgactagtaa aaatcctcgc
    26401 tcgacggtgg gaacgacgac tgaaatcaat gactatctgc gtctcctcta cgcacgtgtg
    26461 gggacgcctt actgtatcaa cggacatgga gctatcaatg cttcttctgt ggagcaaatc
    26521 gttgataagg ttttggagtt acctgaacgc cagcgcttgc agatcttggc tcctgtcatc
    26581 cgcaagaaaa aaggccaaca taagagtgtt atcgagaagg ttcagaaaga cgggtatgtt
    26641 cgtgttcgtg tggatgggga agtctatgat gtgaccgaag tgccagagtt gtctaagagc
    26701 aagcaacaca atatcgatgt cgtggttgac cgtattgtta tcaaggaggg cattcgtagc
    26761 cgtctctttg attccattga agctgccctt cgtatcgcag aaggttatgt gattatcgac
    26821 accatggacg actctgagtt gttgttctct gagcattatg cctgtccagt ttgtggattt
    26881 actgtcccag agttagaacc tcgtctcttc tctttcaatg ctccctttgg ctcttgtagt
    26941 gagtgtgacg gcttggggat taagctggag gtggatactg atttggtagt gccagatgcc
    27001 agcaaaacct tacatgaggg agcgctggca ccttggaatc ctatctcatc caactactat
    27061 ccaaacatgt tagagcaggc catgaaagtc tttggagtgg ctatggataa gccttttgag
    27121 gacctgtcag aagaagataa gaacttgatt ctctacggct cagatggtaa ggaattccat
    27181 ttccattatg aaaatgaatt tggtggtgtg cgcgatatcg acattccgtt tgagggagtt
    27241 atcaataata tcaaacgtcg ttaccatgaa accaatagcg attacactcg cactcagatg
    27301 cgtctctaca tgaatgagct gacctgcgga acctgtcaag gctatcgtct caatgaccag
    27361 gccttgtctg tccgtgtggg cggccagcaa gggccacata ttggagaaat ctcagacctg
    27421 tctatcgctg accacttgga cttggtgagc cagttaactt tgtctgaaaa tgaagccatc
    27481 attgctcgtc ccattctcaa ggaaatcaag gatcgtttga ccttccttaa taatgtgggt
    27541 cttaactatc taaccctgtc acgttcagca ggaacccttt caggtgggga aagtcagcgc
    27601 attcgtttgg caacccagat tggttccaac ctatcaggtg tcctctatat cctagacgag
    27661 ccgtcaatcg gtcttcacca gagggacaat gaccgcctga ttgccagtct gaaaaagatg
    27721 cgtgacttgg gcaatactct tatcgtggtg gaacacgacg aagataccat gcgtgaagct
    27781 gattatctga ttgacgttgg tcctggtgcc ggtgtttttg gtggtgagat tgttgcagca
    27841 ggtacaccta agcaagtggc tcgcaacagc aagtctatca caggtcagta cttgtcaggc
    27901 aaacgtgtca tcccagtacc agaagagcgc cgtgtcggaa atggtcgttt tattgaagtg
    27961 acaggagcgc gtgagaacaa cttgcaaaat gtcacggctc gctttccact aggaaaattt
    28021 atcgcagtga caggtgtgtc gggttcaggg aaatcgactc taatcaacag cattctcaaa
    28081 aaagccattg cccagaagct caaccgtaat tcagacaaac ctggtaagtt taagactatt
    28141 acagggattg agcatgtcga ccgtttgatt gatattgacc agagccctat cggacggacg
    28201 ccgaggtcta acccagctac ctatacagga gtttttgacg atatacgtga cctttttgct
    28261 cagacaaatg aagccaagat tcgaggctac aagaaggggc gcttcagttt caacgtcaag
    28321 ggaggtcgct gtgaagcttg ctcaggtgac gggattatca agattgagat gcacttcttg
    28381 ccagatgttt atgtggcttg tgaagtttgc cacggaaccc gctataacag tgaaacccta
    28441 gaagttcact acaaggaaaa gaatatttcg caggtcttgg atatgacggt caacgatgcg
    28501 gtagaatttt tccaacacat tccgaaaatt caacgcaaac tccagaccat caaggatgta
    28561 ggtctaggct atgtgacctt gggacagcca gctaccaccc tttctggggg agaagcccaa
    28621 cgtatgaagt tagctagcga actccacaaa cgctcgacag gaaaatcttt ctacattctg
    28681 gatgagccga cgacagggct tcatacagag gacattgctc gcctgctcaa ggttttagct
    28741 cgctttgtag acgccggcaa tacagtcctc gtcatcgagc acaatctgga tgttatcaag
    28801 acggcagacc atatcattga cttgggacct gagggcggtg tcggtggtgg aaccatcatc
    28861 gtaacaggaa ctccagaaga agtagctgcc aacgaagcca gctatacagg acactatttg
    28921 aaaggaaagt tacatcatga ataaacgcgt acaagcattt ctagctaaaa tgcaagaaaa
    28981 agaactagat ggtatcatca tcaacaatct taaaaacgtc tattatttga ctggtttttg
    29041 gggctcaaac ggaacagtct ttatcagccg tgaccgtcag gtcttagtga cagactctcg
    29101 ctatatcatc gcagctaagc aagaaaccag tggttttgag attgtggctg atcgtgatga
    29161 attggctgtc attgcaggaa ttgttaagga catgaacttg actcgtatcg gttttgaaga
    29221 tgagatttca gtgtcttatt accaccgtat gcaggcagct tttgcaggtt tgaacttgct
    29281 tccacaaact cagtttgtgg aaggtcttcg tatgattaag gatgaggcag agattgcagc
    29341 gattcgcaag gcttgttcta tctcagacca agctttccgc gatgcgcttg actttatcaa
    29401 accaggaaaa actgaaattg agattgccaa cttccttgat ttccgcatgc gtgagttggg
    29461 agcatctggc ttatcttttg atacgatcct agctagcggt atcaattctt ctaaacccca
    29521 tgcccatcca atgcacaaac cagtggagtt gggagaagcc attaccatgg acttcggctg
    29581 tctctatgac cactatgtca gtgatatgac ccggactatc tatctagggc atgttagcga
    29641 tgagcaggca gagatttaca atacggttct aaaagctaac caagccttga ttgaccaagc
    29701 taaggcaggc ttaggtttcc gtgactttga caaaatccct cgtgatatta tcattgaggc
    29761 aggttatggt gactacttta ctcacggcat tggccacggt attggtctgg atatccatga
    29821 ggaaccctac tttagtcaga cttctacaga aactattaag acaggtatgg ccttgaccga
    29881 tgaaccaggt atctatatcg aaggcaaata tggcgttcgt atcgaggatg atatcctgat
    29941 tacagagaca ggttgtgaat tattgaccct agctccaaaa gagttgatag tcatttagtt
    30001 gtttgtcaag aaaaaagtac gaaaatgacg aaaaaagtca aaaaaaatta aaaataggtc
    30061 gcaagtcgga tgttttttat ggtataatag actaaactga tagtaacatg tagcgaaagg
    30121 ggtaggtaca tgattaaaat ttatacagtc tcaagttgta ctagttgtaa aaaagcaaaa
    30181 acctggctca atgcccacca gttaagttat aaagaacaaa accttggtaa agaaggaatt
    30241 acgagagaag aattactgga tattctaacc aaaacagata acggaatagc cagcatcgtt
    30301 tcgtctaaaa atcgctatgc caaagccctt ggagtggata ttgaagattt gagtgtcaat
    30361 gaagttctca atctgattat ggaaacaccg agaattttaa agagcccaat ccttgtagat
    30421 gaaaaacgcc tgcaagttgg ctacaaggaa gacgatattc gtgccttcct accacgctct
    30481 gtccgtaatg tagaaaatgc agaagcacgt ttgcgtgcag ctctataaac atcaaggctg
    30541 ggagcacttc ccagtcttat tctattttaa tttcaaaaag aaagaagaaa gaaatgaaaa
    30601 aaatagttct tgttagtcta gctttccttt ttgtcctggt tggttgcgga cagaaaaaag
    30661 aaactggacc agctacaaaa acagaaaaag atacgcttca gtcggcattg ccagttattg
    30721 aaaatgctga gaagaataca gttgtaacta agactttggt cttgcccaag tcagatgatg
    30781 gtagccagca gacacaaaca attacttaca aagacaagac ttttttgagt ctagctatcc
    30841 aacaaaaacg tccagtctct gatgagttga agacttatat tgaccaacat ggagtggagg
    30901 aaactcaaaa agctcttctt gaagcggagg agaaggataa gtctatcatt gaagctcgta
    30961 aattggcagg tttcaaactt gaaacaaaac tattgagcgc aacggaactt caaacaacga
    31021 ctagttttga ttttcaagtt ctggatgtca agaaggcttc ccagttggaa catctgaaga
    31081 atattggttt ggaaaatctt ttgaaaaatg aaccaagcaa atatatttca gatagattgg
    31141 caaatggcgc gacagaacaa tagaaaatcc aaataaatag actgcctgaa ttgtagaagg
    31201 taaggaatta tgctataatg gtactattct aaggaaagag ggtgttagaa tgggatttac
    31261 tgaagaaaca gtacgtttta aattggacga ttccaataaa aaagaaatta gcgaaacttt
    31321 gacagatgtt tatgcttcgt tgaacgataa gggttacaac ccaattaacc aaatcgtagg
    31381 ttacgtattg agtggagacc ctgcctacgt tcctcgttat aataatgcac gaaatcaaat
    31441 ccgtaagtat gagcgtgatg aaatcgttga ggaattggtt cgctactacc tcaaaggaca
    31501 aggagtcgat ctataaccta tgagaattat gggattggac gtcggttcaa aaacggtagg
    31561 ggtggcgatt agcgatccgc ttggttttac agctcaaggg cttgaaatca tccagataaa
    31621 tgaagaacaa ggccaatttg gttttgaccg cgttaaggaa ttggttgata cttacaaggt
    31681 ggaacgattt gtagtgggct tgcctaaaaa catgaacaat acaagtggac cgcgcgtaga
    31741 agctagtcaa gcctacggag caaagctaga agagtttttt ggtttaccag tagactatca
    31801 ggatgaacgc ttgacaacag tggctgctga gcgcatgttg attgaacaag cagatatcag
    31861 tcgcaataag cgcaagaaag tcattgataa gttagcagct cagctgattt tacaaaatta
    31921 tttagataga aaattttaat ataaaggaga ggctatgtca cacgatcata accacgacca
    31981 cgaagaacgt gaactaatca cactagtaga tgagcaagga aatgaaacct tgtttgaaat
    32041 ccttttgaca attgatggaa aagaagaatt tggtaaaaac tatgttcttc tagtaccagt
    32101 taacgcagaa gaagacgaag acggacaagt tgaaatccaa gcttactcat tcattgaaaa
    32161 tgaagatgga acagaaggcg aattgcaacc aatcccagaa gactcagaag acgaatggaa
    32221 catgattgaa gaagtcttca acagctttat ggaggagtga tacagtctgg gagactgtat
    32281 cagcccaact ccagaaatgg ggaagagttg gaacgtccag tgaacatttt taccccaact
    32341 cttagaaatt gagaagagtt ggaacatcta gtggatgttt ttagcccata ttcacattca
    32401 tagtagctag ttattagcta accaggtaag aggtcgggac gaaaatcctg gccttgtttt
    32461 tattagtaac catactttaa tgcgataatt gattggactt ttgtcaagga gatgtgtatg
    32521 tttgaagtag aagaatggct ccatagtcgg attggtttga attttcgatc aggtttgggc
    32581 cgaatgcagc aagcggtgga tttgttagga aatcctgagc agtcttaccc tattatccac
    32641 gtaacaggga ctaatgggaa aggatctacc attgctttta tgagggaatt atttatgggg
    32701 catggcaaaa aagttgcgac ctttacctcc cctcatatcg tctctatcaa tgaccgaatc
    32761 tgcattaatg ggcaacctat agcagacgca gactttatcc gtttgactga tcaggtcaag
    32821 gagatggaga aaacgcttct gcaaactcct gcccagttgt ccttttttga attgctgacc
    32881 ttagttgctt ttctttattt tagggagcaa gaggtggatt tggttttatt agaagtggga
    32941 attggtggct tacttgacac gaccaatgtg gtaactggag agcttgctgt catcacctcc
    33001 attgggcttg accatcaaga aaccttgggt gatagtctag aagcaattgc agagcagaaa
    33061 gctggtattt tcaaggctgg taaaaaggca gtgattgcga aattgcctcc agaagctaga
    33121 cttgtctgtc agaaaaaagc cgaatcttta gctgttgacc tttatcaggc aggtcaagat
    33181 ttttcagtgc tgaatggtga tttttcaagc tctttactaa atctttcaca gctgaacata
    33241 ggcttagaag gagcctatca gcaggagaat gcggccttgg cgttgcaaac ttttcttctt
    33301 tttatgggag aaagaaagga agctattgat gaacaggctg taagaaaggc cttggaacag
    33361 acccattggg ctggtcgctt ggagcgtatt cgcccacaga tttacttgga tggtgctcat
    33421 aacctccctg ccttgactcg cttggttgag tttatcaaag aaaaagagca ggaaggctat
    33481 cgacctcaaa tcctctttgg atccttgaaa cgtaaggatt atcaagggat gttgggttat
    33541 ctgactgaaa atttacctca agtggaactc aaaatgaccg gctttgacta tcaagggtct
    33601 ttggacgaga cagatgtaac aggttaccat gtaattccct cttaccgaga atttatcagc
    33661 agtttcgaag aaagggcaga cgctcaagac ttgctgttcg ttacagggtc tctctatttt
    33721 atctcagaag tacggggcta cctgctggac cgtgagcaga taaattgacc cccttttttg
    33781 aacttgttat actaggggaa ttgctggtta gagaaaattt ctctaaattg gtagcagaaa
    33841 ggaaattcat catgaaatta aaaagattca cactttctct tgcttctcta gcaagtttta
    33901 gtctcttagt agcttgttca caaagagctc aacaggttca acagcctgtt gctcagcagg
    33961 tccaacagcc tgctcaacag aataccaata ctgcaaatgc aggaggtaac caaaatcaag
    34021 cggctccagt acaaaaccaa cctgttgctc aaccgaccga tattgatggg acttatactg
    34081 gtcaggatga cggagaccgt atcactttag tggtaactgg aacgactggt acatggactg
    34141 agctcgaatc tgacggggat cagaaagtca aacaggttac atttgattca gcaaatcaac
    34201 gcatgattat tggcgatgat gtcaaaattt acactgtaaa cggtaatcaa atcgtcgtag
    34261 atgatatgga tagagaccca tcggaccaaa tcgttttaac taaataagac tatgcaagta
    34321 gaaagggctg gatttttcag cctttttact tttacctaga aaataatcat aaatacttgc
    34381 cttctaccga atacctgcgt tatactagta tcaattactt gtttttagca ttcaaactat
    34441 caaggagggg atatgaaata taggaaattt caattattga tgtccaagta tggctttagt
    34501 ctttcgatta tgctacttga actttgtctt gtttttggtc tctttcttta tttaggacgc
    34561 atggctccca ttttatggat tactgtcctc attctactga gtatcatcac aatcatttcg
    34621 atagtcaacc gtaatacgac tcctgagaat aaggtaacct ggttgttagt agcctttgtg
    34681 ccagtatttg gtcccttgct ctatctgatg tttggtgaaa ggcgattgtc caaaaaagaa
    34741 atcaaacaac tgaagaagct aggctctatg catttccaag aagcaaatag ccagctacta
    34801 aaagagaaat taaaagaaag tgacaaggca gcttatggag tcatcaagtc cttattgagt
    34861 atggatacca atgctgacat ctatgatcaa actgcctcta cattttttcc taacggagaa
    34921 gctatgtgga aaaagatggt agaagatctt aaaaaggctg agaaatttat tttcttggaa
    34981 tattacatta tagaagaagg tttgatgtgg aatcgcatac tagatatact agagcaaaag
    35041 gtagctcagg gtgtagaggt taagatgctc tatgatgata tcggctgtat ggctacttta
    35101 acaggagatt atgcacatcg acttcgtcag ctgggcatcg aggcccataa attcaataaa
    35161 gttattcctc gtttgacagt ggcttataat aacagagatc atagaaaaat attgattgtt
    35221 gatggtcaga tagcctatac tggtggggtc aatctggcag atgagtacat taaccacgtc
    35281 gagagatttg gttattggaa ggatagtgga attcgcttag acggactagc agtaaaagcc
    35341 ctgacacgct tatttttgac cacttggtac attaatcgag gagaaattag tgattttgat
    35401 caatatcatt tagaaaatca ttctatcccg agtgacggtt taaccattcc atacggaagt
    35461 ggacccaagc caatttttcg agcgcaggta gggaaaaaag tttatcagag tttaatcaat
    35521 caagcaacag aatcggtcta tattacgaca ccttatttga ttatagatta tgatttaaca
    35581 gagacaatca aaaatgcagc tatgagaggg gtcgatgttc gaattatcac cccttacata
    35641 ccagataaga agttcattca gttagtcacg agaggagctt atcccgacct tctttctgct
    35701 ggtgttcgga tttatgagta tagtccaggt tttattcata gtaagcagat gttggtagac
    35761 gaagattttg cggtggtggg gacaatcaat ctcgactacc ggagcttggt acaccattat
    35821 gaaaatgcag tcttactcta taaaactcct tctataaggg aaatcgcccg agattttcga
    35881 aatatatttg cagattctca ggaagtctat cctcattcta tcaaaacgag ctggtatcaa
    35941 aagcttgtaa aagaaatcgc ccagctattc gcccctatct tataagaaat ctagaactta
    36001 ggatacaaac cagacctatt tttcttgtct tttactaata gaaagaaaga ggaggggaat
    36061 tcatgctaac taaggaagaa gtgaatgata tgattgagtt caaacagaca catttatacc
    36121 gctttcggga aattgaaacc tttgtgaaac tatgcaaaaa atcactcaaa cagccatcgc
    36181 aagctgacaa tcctaggact ttttgatata ctaagacaga taaattgaac aggagaaacg
    36241 atatgagtgt gtatggtaga gtagaagaag ttcataagga gaatcgtgaa cctctagaat
    36301 accaaatcga acaagaatcg catcatcgtg aatcaagtcg tcttcctttg gtgaaaattt
    36361 tactatggag tacgcttgta acggggataa ctctaggagt accgctatta ctcgacttaa
    36421 tgagtgcaca agaagtgcag gatttttatg caggttgggg gcttcatcag acagggaaga
    36481 tttacagcga ctattatgga agtcaaggtt tgctttatta tttgctgact tacgtgagtc
    36541 agggcggatt tttctttgcc atctttgagt ggttagcctt ggtagcagga ggatttttcc
    36601 tttttagatc agcggacacc ttgacagagc aaggagacca agctggacat ctggtgacta
    36661 ttttttacat gctagttaca ggtcttgctt ttggtggagg ctatgcgact cttttagcgc
    36721 ttcctttctt attcgcagcc tttagtttag ttgcggctta cctaagcaat ccaagccatg
    36781 ataagggatt tgtacggatt gggctagctt tggcaggcgg atttttcttt gctcccttat
    36841 catcgctcct gtttattgct gtagtgagtt taggcttgtt ggtctttaac cttgggcata
    36901 gacgctttgc gcatgggttt tatcagtttc ttgcagtggc tttaggtttt tcacttgtct
    36961 tttatccaac tgcctactat agtgctgcaa caggaagttt tggggatgcg attagtggta
    37021 ttcgttatcc tattgacagt attcgctttg attttacttc taaaatttta gagaatatgt
    37081 ttttttatgg cttgttgtcc cttggtttgg gatttgtggt ttgtatcttt ttaggtctct
    37141 ttcaatccaa accctttaaa ctatacgtca tttcggttcc tgcaagtctt gtggtaattt
    37201 taggtttgat tttacttttc ttttcacaag agcctctgca cgcttcttat ttgatggtcg
    37261 tcttccctgt tttcctactt ttattggtaa ccaatattaa gagtcaacag agggggcgta
    37321 gtgctagaag aagccgaaga gaaacgccag ttagcctatg gagtcgtttc ttcaaaggaa
    37381 atctatatct gctagttttt gggtttgtct atcttttgtc tgttcctttt ttgatgaagt
    37441 ttgtccttta tccagtacct tatcaagaac gtaatcgtct tgctgatttg gtaaaagagg
    37501 agacaaatac ggaagatgct atctatgcat gggatgatac tgcgactctt tatcgtaaga
    37561 gtgagcgctt gtcgccatcg gcgattttgt ccccgttgca ctatacagca actgaggaaa
    37621 atcgtaataa gttacttaat gacttgaaag aaaaacaacc taaggtgatt gtggtaaatg
    37681 acaaggtggt agtctggtct gaagtggaaa cacttttaaa agaaaattac caacaagtaa
    37741 agactgatta ctcagagttt aaagtctata aaattaaata accaaatcaa tatcttgtgt
    37801 atttttaaaa attttaggat ttttaacaca agatattgat ttttcttttt agagtggtat
    37861 aatacttttt agaaagaaca ttttagaaaa gagcatgcgt atgattgcac tagaagaaaa
    37921 aattacaatt ttgccaactc tcttcgtcga gaaacgagat gggagacgtg ttgtatttga
    37981 tgtggacaag attgacaagg ctctccacaa ggcggctgac aaggttatgg atgtgacacc
    38041 cctggttgaa aaacgcctca atgatctgac tgagcgaatt attacagaaa ttcatagtcg
    38101 ctttccacag ggaattaaga tttacgaaat tcaaaatatc gtagaacatg aactccttga
    38161 agccaaagaa tatgcgctgg ctgaggagta tattacttat cggacacaga gggattttga
    38221 gcgctcaaaa gcgacggata tcaactttag tattcataaa cttctcaaca aagaccagac
    38281 agttgtcaat gaaaatgcta ataaagacag tgatgtcttt aacactcagc gtgatttgac
    38341 agcagggatt gttgggaaat caatcggact gcaaatgctt cctaagcacg tagccaatgc
    38401 ccaccaaaag ggggatatcc actatcacga tttggactac agtccctata cccctatgac
    38461 caactgctgt ttgattgatt ttaagggtat gttggaaaat ggttttaaga ttggaaatgc
    38521 agaggtagag agtcccaagt ctatccagac tgcgacagca cagatttctc aaatcattgc
    38581 caacgttgct tctagccagt acggtggctg ttcagctgac cgtatcgatg aaattttggc
    38641 gccttatgca gagaagaatt atcaaaaaca tctcaaagat gcagaagagt gggtattgcc
    38701 tgaaaaacgg gaagattacg cttggaagaa aacgcaaaag gacatctacg atgccatgca
    38761 atctcttgag tatgaaatca atactctctt cacttcaaat ggacaaacac cttttacttc
    38821 gttaggtttt ggtctgggaa ccagtcgttt tgaacgagaa attcaaaaag ctattttaaa
    38881 cattcgcatc aagggtcttg gttcagaaca ccgtacggct atctttccta aacttatttt
    38941 cactctgaaa agaggcctta atttagagga aggaactccc aactacgaca tcaagcagtt
    39001 ggctcttgag tgtgcaacca aacggatgta tccagatgtt ttatcttacg ataagattgt
    39061 tgacttgaca ggatccttca aggtgcctat gggttgccgt tctttcctcc aaggatggaa
    39121 ggatgaaaat ggtgtagaag tcaattcagg tcgcatgaat ctgggtgttg tgacggttaa
    39181 tctgcctcgt attgctcttg agtctgaagg tgatatgaat aagttctggg aaatcttcaa
    39241 cgagcgaatg aatatcgcag aagatgctct tgtttaccgt gtcgaacgca ctaaagaggc
    39301 gacaccagca aatgctccta ttctttatca gtacggtgct tttggccatc gtctaggtaa
    39361 agaagaaagt gttgaccagc tctttaagaa tcgtcgtgct accgtttcgc tgggctatat
    39421 cggcttgtat gaagtagcga cagttttctt tggtaacagc tgggaaagta atccagatgc
    39481 taaggaattc acgctagaca tcattcacga tatgaaacgc cgtgtagaag agtggtcaga
    39541 ccaatatggc taccatttct ctatctactc aacaccatcc gaaagtctga cagaccgttt
    39601 ctgtcgacta gatatagaca agtttggctc tattcctgat atcacagaca aggaatacta
    39661 caccaactct ttccactacg atgttcgtaa aaatccaaca ccgtttgaaa aattggactt
    39721 tgagaaagtc tatccagaag caggtgcgtc aggtggtttc atccattatt gtgagtatcc
    39781 agtccttcag caaaatccaa aggccttgga agctgtctgg gattatgctt atgaccgtgt
    39841 aggctatcta ggcaccaata ctccgattga ccgttgctac aagtgtgact ttgaagggga
    39901 ttttgaacca actgagagag ggtttgcttg tccaaactgt ggcaatagcg accctaaaac
    39961 agtagatgtg gtgaagcgaa cttgtggcta cctaggtaat cctcaagcaa gaccgatggt
    40021 caacggccgt cacaaggaaa tcgctgcgcg tgtcaaacat atgaatggtt caacgattaa
    40081 aatagctggg catcaagtaa caaattagaa agaaatgaaa tgggaaaata tcaactagac
    40141 gataaggggc gcgcacaagt gacccgttat cacgagaaac actctaaagg tggagctggt
    40201 aagaaagaac gcttgcttag cttcagagaa caatttttaa acaagaacaa gaaaaaataa
    40261 aagtgagagc cagctctcgc ttttctcata gtgggaggta aggatggaat tacgcagacc
    40321 aagattagcg gataagaaag ctgttttaga tatgatgaca gagtttgaaa aatttcagtc
    40381 gcctcacgac ggcggtttct gggatacaga gaactttgtg tatgaagact ggttagaaag
    40441 caatcaggaa caggaaatgg ggattaatct gcctgaagga tgggttcctg caattcagtt
    40501 agtggctttt tctgagaaag gtcaagcagt tggatttctt aatctccggt tgcgcctcag
    40561 taactttcta ctagaagaag gtggccacat tggctactcc attcgtccat ctgaaagagg
    40621 caagggttat gcaaaagaga ctctccgtca gggcttgcaa gttgctaagg aaaagaacat
    40681 caagaaagct ctggtgacct gtagcgtgaa taatcctgct agcagagcag tcattctagc
    40741 aaatggtgga atatttgagg atgctcgcaa tggagtcgag cgttattgga tagaggtagc
    40801 gaatgaataa tccaaaacca caagaatgga aaagcgagga acttagtcaa ggtcgtatca
    40861 ttgactacaa ggcctttaac tttgtggacg gcgaaggcgt gcgtaactct ctctatgtat
    40921 caggctgtat gtttcactgc gagggatgtt ataatgttgc aacttggtct tttaatgctg
    40981 gcattcccta tacagcagaa ttagaagagc agatcatggc agatcttgct cagccctatg
    41041 ttcaaggttt gactttgttg ggaggggagc cttttcttaa tactgggatt ctcttgccac
    41101 ttgttaagcg gattcggaag gaattgccag acaaggacat ctggtcctgg accggctaca
    41161 cttgggaaga aatgatgttg gaaactccag ataaactgga attcttgtca ctgattgaca
    41221 ttcttgtcga tggaagatat gatcgaacta agagaaatct tatgctccag tttcgaggtt
    41281 catctaacca acgaattatc gatgtgcaaa aatcgctcaa aagtgggcaa gtagtgattt
    41341 gggacaagct caatgacgga aaagaaagct atgaacaggt gaagagagaa tgaagaaaaa
    41401 atgaagaaaa aagacttagt agaccaacta gtctcagaga tcgagacggg gaaagtcagg
    41461 acactgggaa tatacggtca tggagcttca ggtaaatcaa cctttgcaca ggaattgtac
    41521 caagctttag attctactac agtaaatttg ctagaaacag atccctatat cacctccgga
    41581 cgctatttgg tagtacccaa ggacgcgccg aatcaaaagg tgacagccag tctgccagtg
    41641 gcgcatgaac tggagagttt gcagagagat atccttgcct tgcaggcggg tatggatgtc
    41701 ttgacaattg aagaaccttg gaaggctagt gaggtcttgt ctggagccaa accaattttg
    41761 attgtcgaag ggatgtctgt tggttttcta cccaaggaac tctttgaaaa aaccatctgt
    41821 ttctacacgg atgaggagac cgaattaaag cgacgccttg ctagagatac gactgtgaga
    41881 aatcgctatg catcctttat attggctagc catcagatga gacgggagca gtatctgcga
    41941 tactataaag aaactgagtc taaggcggat atcttagtgg accaatcaga agataaattt
    42001 gatgtcaaga ggacttaact tatgtataga agaaatatag aagaaaatcc ctaattttag
    42061 aaagaaaaag taggaaaaca gtttcatccc aaaaaaacga aaaaacctaa caaatccctt
    42121 gcaatcgcag gggatttgtg ttattctatt atggtgctgt aaattacagc cttagttttg
    42181 atgcaagagg ttgcgacacg ctcggttgca ttgccacgca acaccgcgtc ggttttcttg
    42241 tggagctagc ctattatctt aaatagacga aaaggagaaa aagatggcaa acaaaaaaat
    42301 ccgtatccgt ttgaaagctt acgaacaccg tacgcttgac acagcggctg caaaaatcgt
    42361 agaatcagct actcgtacag gtgcacaagt tgcgggtcca atcccacttc caactgaacg
    42421 tagcctctac acaatcattc gtgcgactca caaatacaaa gactctcgcg aacaatttga
    42481 aatgcgtaca cacaaacgtt tgatcgatat cgttaaccca actcaaaaaa cagttgatgc
    42541 cttgatgaaa ttggatcttc caagtggtgt aaacgtagaa atcaaacttt aatctaaaat
    42601 ataaaagagc agaggctggt gtttcaatct aattgaacac gggctaaact cggtgtgaaa
    42661 aagataaact tcctcgcgtc tgctagacac tgtgtcagtt tcctattttc actttgagtt
    42721 tgacgccctt tgtatcttag acttgagcat aaaaaacgct cgttaaaaac tttttgaata
    42781 aaaaatatag aaaaggaact attttctcat gacaaaagga atcttaggga aaaaagtggg
    42841 aatgactcaa atcttcactg aagctggcga attgatccct gtaacagtta ttgaagcaac
    42901 tccaaacgtt gttcttcaag ttaaaactgt tgaaacagac ggatacaacg ctatccaagt
    42961 tggtttcgat gacaaacgcg aagtattgag caacaaacct gctaaaggac atgtagcgaa
    43021 agctaacacg gctcctaagc gcttcattcg tgaattcaaa aacgttgaag gcttggaagt
    43081 tggtgctgaa attacagttg aaacattcgc agctggagac gttgttgacg taactggtac
    43141 ttctaaaggt aaaggtttcc aaggtgttat caaacgccac ggacaatcac gtggaccaat
    43201 ggctcacggt tctcgttacc accgtcgtcc aggttctatg ggacctgttg cacctaaccg
    43261 cgtattcaaa ggtaaaaacc ttgcagggcg tatgggtggc gaccgcgtaa caattcaaaa
    43321 ccttgaagtt gtacaagttg ttccagaaaa gaacgttatc cttatcaaag gtaacgtacc
    43381 aggtgctaag aaatctctta tcactatcaa atcagcagtt aaagctggta aataataaag
    43441 aaaggggaaa tcagtcacaa tggcaaacgt aacattattt gaccaaactg gtaaagaagc
    43501 tggccaagtt gttcttagcg atgcagtatt tggtatcgaa ccaaatgaat cagttgtgtt
    43561 tgatgtaatc atcagccaac gcgcaagcct tcgtcaagga acacacgctg ttaaaaaccg
    43621 ctctgcagta tcaggtggtg gacgcaaacc atggcgtcaa aaaggaactg gacgtgctcg
    43681 tcaaggttct atccgctcac cacaatggcg tggtggtggt gttgtcttcg gaccaactcc
    43741 acgttcatac ggctacaaac ttccacaaaa agttcgtcgc ctagctctta aatcagttta
    43801 ctctgaaaaa gttgctgaaa acaaattcgt agctgtagac gctctttcat ttacagctcc
    43861 aaaaactgct gaatttgcaa aagttcttgc agcattgagc atcgattcta aagttcttgt
    43921 tatccttgaa gaaggaaatg aattcgcagc tctttcagct cgtaaccttc caaacgtgaa
    43981 agttgcaact gctacaactg caagtgttct tgacatcgca aatagcgaca aacttcttgt
    44041 cacacaagca gctatctcta aaatcgagga ggttcttgca taatgaattt gtatgatgtt
    44101 atcaaaaaac ctgtcatcac tgaaagctca atggctcaac ttgaagcagg gaaatatgta
    44161 tttgaagttg acactcgtgc acacaaactt ttgatcaagc aagctgttga agctgctttc
    44221 gaaggtgtta aagttgccaa tgttaacaca atcaacgtaa aaccaaaagc taaacgtgtt
    44281 ggacgttaca ctggttttac taacaaaact aaaaaagcta tcatcacact tacagctgat
    44341 tctaaagcaa tcgagttgtt tgctgctgaa gctgaataat ctaaggagga aatatcgtgg
    44401 gaattcgtgt ttataaacca acaacaaacg gtcgccgtaa tatgacttct ttggatttcg
    44461 ctgaaatcac aacaagcact cctgaaaaat cattgcttgt tgcattgaag agcaaggctg
    44521 gtcgtaacaa caacggtcgt atcacagttc gtcaccaagg tggtggacac aaacgttttt
    44581 accgtttggt tgacttcaaa cgtaataaag acaacgttga agcagttgtt aaaacaatcg
    44641 agtacgatcc aaaccgttct gcaaacatcg ctcttgtaca ctacactgac ggtgtgaaag
    44701 catacatcat cgctccaaaa ggtcttgaag taggtcaacg tatcgtttca ggtccagaag
    44761 cagatatcaa agtcggaaac gctcttccac ttgctaacat cccagttggt actttgattc
    44821 acaacatcga gttgaaacca ggtcgtggtg gtgaattggt acgtgctgct ggtgcatctg
    44881 ctcaagtatt gggttctgaa ggtaaatatg ttcttgttcg tcttcaatca ggtgaagttc
    44941 gtatgattct tggaacttgc cgtgctacag ttggtgttgt cggaaacgaa caacatggac
    45001 ttgtaaacct tggtaaagca ggacgtagcc gttggaaagg tatccgccca acagttcgtg
    45061 gttctgtaat gaaccctaac gatcacccac acggtggtgg tgaaggtaaa gcaccagttg
    45121 gtcgtaaagc accatctact ccatggggca aacctgctct tggtcttaaa actcgtaaca
    45181 agaaagcgaa atctgacaaa cttatcgttc gtcgtcgcaa cgagaaataa tattaaacta
    45241 gtcgcttaag caactagtaa atccgccagc tcggtagcgc tccataggag tgcaagccgc
    45301 tgtggtacaa catttaaagg agaaaatata aaaatgggac gcagtcttaa aaaaggacct
    45361 ttcgtcgatg agcatttgat gaaaaaagtt gaagctcaag ctaacgacga aaagaaaaaa
    45421 gttattaaaa cttggtcacg tcgttcaacg atcttcccaa gtttcattgg ttacactatt
    45481 gcagtttatg acggacgtaa acacgtacct gtttacatcc aagaagacat ggtaggccac
    45541 aaacttggtg aatttgcacc aactcgtact tacaaaggtc acgctgcaga cgacaagaaa
    45601 acacgtagaa aataaggaga acataaatgg cagaaattac ttcagctaaa gcaatggctc
    45661 gtacagtacg tgtttcacct cgtaaatcac gtcttgttct tgataacatc cgtggtaaaa
    45721 gcgtagccga tgcaatcgca atcttgacat tcactccaaa caaagctgct gaaatcatct
    45781 tgaaagtttt gaactcagct gtagctaacg ctgaaaacaa ctttggtttg gataaagcta
    45841 atttggtagt atctgaagca ttcgcaaacg aaggaccaac tatgaaacgt ttccgtccac
    45901 gtgcgaaagg ttcagcttca ccaatcaaca aacgtacagc tcacatcact gtagctgttg
    45961 cagaaaaata aggaggtaaa atcgtgggtc aaaaagtaca tccaattggt atgcgtgtcg
    46021 gcatcatccg tgattgggat gccaaatggt atgctgaaaa agaatacgcg gattaccttc
    46081 atgaagatct tgcaatccgt aaattcgttc aaaaagaact tgctgacgca gcagtttcaa
    46141 ctattgaaat cgaacgcgca gtaaacaaag ttaacgtttc acttcacact gctaaaccag
    46201 gtatggttat cggtaaaggt ggtgctaacg ttgatgcact ccgtgcaaaa cttaacaaat
    46261 tgactggaaa acaagtacac atcaacatca tcgaaatcaa acaacctgat ttggatgctc
    46321 accttgtagg tgaaggaatt gctcgtcaat tggagcaacg tgttgctttc cgtcgtgcac
    46381 aaaaacaagc aatccaacgt gcaatgcgtg ctggagctaa aggaatcaaa actcaagtat
    46441 caggtcgttt gaacggtgca gatatcgccc gtgctgaagg atactctgaa ggaactgttc
    46501 cgcttcacac acttcgtgca gatatcgatt acgcttggga agaagcagat actacatacg
    46561 gtaaacttgg tgttaaagta tggatctacc gtggtgaagt tcttccagct cgtaaaaaca
    46621 ctaaaggagg taaataacca atgttagtac ctaaacgtgt taaacaccgt cgtgagttcc
    46681 gtggaaaaat gcgcggtgaa gcaaaaggtg gaaaagaagt agcattcggt gaatacggtc
    46741 ttcaagctac aactagccac tggatcacta accgccaaat cgaagctgct cgtatcgcca
    46801 tgactcgtta catgaaacgt ggtggtaaag tttggattaa aatcttccca cacaaatcat
    46861 acactgctaa agctatcggt gtgcgtatgg gatctggtaa aggggcacct gaaggttggg
    46921 tagcaccagt taaacgtggt aaagtgatgt tcgaaatcgc tggtgtatct gaagagattg
    46981 cacgtgaagc gcttcgactt gctagccaca aattgccagt taaatgtaaa ttcgtaaaac
    47041 gtgaagcaga ataaggagaa ggcatgaaac ttaatgaagt aaaagaattt gttaaagaac
    47101 ttcgtggtct ttctcaagaa gaactcgcga agcgcgaaaa cgaattgaaa aaagaattgt
    47161 ttgaacttcg tttccaagct gctactggtc aattggaaca aacagctcgc ttgaaagaag
    47221 ttaaaaaaca aatcgctcgt atcaaaacag ttcaatctga agcgaaataa tagactaggg
    47281 aaggagaaat ttcaatggaa cgcaataatc gtaaagttct tgttggacgt gttgtatctg
    47341 acaaaatgga caagacaatc acagttgtag ttgaaacaaa acgtaaccac ccagtctatg
    47401 gtaaacgtat taactactct aaaaaataca aagctcatga tgaaaacaat gttgccaaag
    47461 aaggcgatat cgtacgtatc atggaaactc gcccgctttc agctacaaaa cgtttccgtc
    47521 ttgtagaagt tgttgaagaa gcggtcatca tctaatcaaa cctgaaagga gaaaactgaa
    47581 atgattcaaa cagaaactcg tttgaaagtc gcagacaaca gcggtgctcg cgaaatcttg
    47641 actatcaaag ttcttggtgg ttcaggacgt aaatttgcaa acatcggtga tgttatcgtg
    47701 gcatctgtaa aacaagctac tcctggtggt gcggttaaaa aaggtgacgt tgttaaagca
    47761 gttatcgttc gtactaaatc aggtgctcgt cgtgctgatg gttcatacat caaatttgac
    47821 gaaaacgcag cagttatcat ccgtgaagac aaaactcctc gcggaacacg tatctttggc
    47881 ccagttgcac gtgaattgcg tgaaggtggc ttcatgaaga tcgtgtcact tgctccagaa
    47941 gtactttaat ttttaggaac aaactagtcc cctagcttca agctagggtg cccttatggg
    48001 cgtaagaaaa atcaaggaga aacctaatgt ttgtaaaaaa aggcgacaaa gttcgcgtaa
    48061 tcgctggtaa agataaggga acagaagctg ttgtccttac tgcccttcca aaagtaaaca
    48121 aagttatcgt tgaaggtgtt aacattgtta agaaacacca acgtccaact aacgagcttc
    48181 ctcaaggtgg tatcatcgag aaagaagcag ctatccacgt atcaaacgtt caagttttgg
    48241 acaaaaatgg tgtagctggt cgtgttggtt acaaatttgt agacggtaaa aaagttcgct
    48301 acaacaaaaa atcaggcgaa gtgcttgatt aatcacgaag gaaaggagaa gtataatggc
    48361 aaatcgttta aaagaaaaat atcttaatga agtagttcct gctttgacag aacaattcaa
    48421 ctactcatca gtgatggctg tgcctaaagt agataagatt gttttgaaca tgggtgttgg
    48481 tgaagctgta tcaaacgcta aaagccttga aaaagctgct gaagaattgg cacttatctc
    48541 aggtcaaaaa ccacttatca ctaaagctaa aaaatcaatc gccggcttcc gtcttcgtga
    48601 aggtgttgcg atcggtgcaa aagttaccct tcgtggtgaa cgtatgtacg aattcttgga
    48661 taaattggta tcagtttcac ttccacgtgt acgtgacttc cacggtgttc caacaaaatc
    48721 atttgatgga cgcgggaact acacacttgg tgtgaaagaa caattaatct tcccagaaat
    48781 caacttcgat gacgttgaca aaactcgtgg tcttgacatc gttatcgtaa caactgctaa
    48841 cactgacgaa gagtcacgtg cattgcttac aggccttgga atgccttttg caaaataata
    48901 taggaggtaa atctaatggc taaaaaatca atggtagcta gagaggctaa acgccaaaaa
    48961 attgttgacc gttatgctga aaaacgtgct gcattaaagg cggcagggga ctacgaaggt
    49021 ttatctaaat tacctcgcaa cgcttcaccg actcgtttac ataatcgttg tagagttacg
    49081 gggcgcccac attcagttta ccgcaaattt ggtctgagtc gtatcgcttt tcgcgaactt
    49141 gcgcataaag gtcaaattcc tggtgtaaca aaagcatctt ggtaatttaa gatatcaagg
    49201 gcgtcaaaac tccaagtaaa aataggaaac ttgacgaaga aactaaagtt tctaggaaag
    49261 tttatctttt tcacacagag tttagcccgg gttcaattgg gcttgccaat ttgaacacga
    49321 gctacagctt tggcaaaaaa gaccaatttg ctttggagca ttgcttctgc attaaattgt
    49381 ctatttttgc tcgtgctgtt acgctctttg tatcatgtat taactagcaa gtgcaacttg
    49441 caaactacta gtaagaggag aaaaacaaaa tggttatgac tgacccaatc gcagacttcc
    49501 taactcgtat tcgtaatgct aaccaagcta aacacgaagt acttgaagta cctgcatcaa
    49561 acatcaaaaa agggattgct gaaatcctta aacgcgaagg ttttgtaaaa aacgttgaaa
    49621 tcatcgaaga tgacaaacaa ggcgtcatcc gtgtatttct taaatacgga ccaaatggtg
    49681 aaaaagttat cactaacttg aaacgtgttt ctaaaccagg acttcgtgtc tacaaaaaac
    49741 gtgaagacct tccaaaagtt cttaacggac ttggaattgc catcctttca acttctgaag
    49801 gtttgcttac tgataaagaa gcacgccaaa agaatgttgg tggtgaggtt atcgcttacg
    49861 tttggtaaaa tcaagataca aagctcgtaa agaacaaagc aaaattagga agttggagaa
    49921 gtttgtttac aaacaagcca acttatctat tttgcacagt tcttagagcg tgttcagttc
    49981 agctcttgaa ctaaataagt atctgaaccc cgtgaaaact ggccgttttg gcctgacaat
    50041 ttaacaggag aaaataaaca tgtcacgtat tggtaataaa gttatcgtgt tgcctgctgg
    50101 tgttgaactc gctaacaatg acaacgttgt aactgtaaaa ggacctaaag gagaacttac
    50161 tcgtgagttc tcaaaagata ttgaaatccg tgtggaaggt actgaagtaa ctcttcaccg
    50221 tccaaacgat tcaaaagaaa tgaaaactat ccacggaact actcgtgccc ttttgaacaa
    50281 catggttgtt ggtgtatcag aaggattcaa gaaagaactt gaaatgcgtg gggttggtta
    50341 ccgtgcacag cttcaaggat ctaaacttgt tttggctgtt ggtaaatctc atccagacga
    50401 agttgaagct ccagaaggaa ttacttttga acttccaaac ccaacaacaa tcgttgttag
    50461 cggaatttca aaagaagtag ttggtcaaac agctgcttac gtacgtagcc ttcgttcacc
    50521 agaaccatat aaaggtaaag gtatccgtta cgttggtgaa ttcgttcgcc gtaaagaagg
    50581 taaaacaggt aaataatgtt gagtggttga tcatcaacca ccaacctatt ttccaacttt
    50641 gtgcatagca cacgatttaa aactaaagag gtgaaaactg tgatttcaaa accagataaa
    50701 aacaaactcc gccaaaaacg ccaccgtcgc gttcgcggaa aactctctgg aactgctgat
    50761 agcccacgtt tgaacgtatt ccgttctaat acaggcatct acgctcaagt gattgatgac
    50821 gtagcgggtg taacgctcgc aagtgcttca actcttgata aagaagtttc aaaaggaact
    50881 aaaactgaac aagccgttgc tgtcggtaaa ctcgttgcag aacgtgcaaa cgctaaaggt
    50941 atttcagaag tggtgttcga ccgcggtgga tatctatatc acggacgtgt gaaagctttg
    51001 gctgatgcag ctcgtgaaaa cggattgaaa ttctaatagg aggacactag aaaatggcat
    51061 ttaaagacaa tgcagttgaa ttagaagaac gcgtagttgc tgtcaaccgt gttacaaaag
    51121 ttgttaaagg tggacgtcgt cttcgtttcg cagctcttgt tgttgttggt gaccacaatg
    51181 gtcgcgtagg atttggtact ggtaaagctc aagaagttcc agaagcaatc cgtaaagcag
    51241 tagatgatgc taagaaaaac ttgatcgaag ttcctatggt tggaacaaca atcccacacg
    51301 aagttctttc agaattcggt ggagctaaag tattgttgaa acctgctgta gaaggttctg
    51361 gagttgccgc tggtggtgca gttcgtgccg ttgtggaatt ggcaggtgtg gcagatatta
    51421 catctaaatc acttggttct aacactccaa tcaacattgt tcgtgcaact gttgaaggtt
    51481 tgaaacaatt gaaacgcgct gaagaaattg ctgcccttcg tggtatttca gtttctgatt
    51541 tggcataaga aaggggataa aatggctcaa attaaaatta ctttgactaa gtctccaatc
    51601 ggacgcattc catcacaacg taaaactgtt gtagcacttg gacttggcaa attgaacagc
    51661 tctgttatta aagaagataa cgctgctatc cgtggtatga ttacagcagt atctcactta
    51721 gtaacagttg aagaagtaaa ctaatgaagt tttaggggat gtgcactgta ccatccccta
    51781 aaactagata tagtcatcta tgatgacatc gtataggcga gttgatgggg gagacaacct
    51841 tttctccctt atcggcgcta gcattttaca aaagaggaga aaataaaaat gaaacttcat
    51901 gaattgaaac ctgcagaagg ttctcgtaaa gtacgtaacc gcgttggtcg tggtacttca
    51961 tcaggtaacg gtaaaacatc tggtcgtggt caaaaaggtc aaaaagctcg tagcggtggc
    52021 ggagttcgcc ttggttttga aggtggacaa actccattgt tccgtcgtct tccaaaacgt
    52081 ggattcacta acatcaacgc taaagaatac gcaattgtga accttgacca attgaacgtc
    52141 tttgaagatg gtgctgaagt aactccagtt gttcttatcg aagcaggaat tgttaaagct
    52201 gaaaagtcag gtattaaaat tcttggtaac ggtgagttga ctaagaaatt gactgtgaaa
    52261 gcagctaaat tctctaaatc agctgaagaa gctatcactg ctaaaggtgg ttcagtagaa
    52321 gtcatctaag agaggtgacc tatgtttttt aaattattaa gagaagctct taaagtcaag
    52381 caggttcgat caaaaatttt atttacaatt tttatcgttt tggtctttcg tatcggaact
    52441 agcattacag ttcctggtgt gaatgccaat agcttgaatg ctttaagtgg attatccttc
    52501 ttaaacatgt tgagcttggt gtcggggaat gccctaaaaa acttttcgat ttttgcccta
    52561 ggagttagtc cctatatcac cgcttctatt gttgtccaac tcttgcaaat ggatatttta
    52621 cccaagtttg tagagtgggg taaacaaggg gaagtaggtc gaagaaaatt gaatcaagct
    52681 actcgttata ttgctctagt tctcgctttt gtgcaatcta tcgggattac agctggtttt
    52741 aataccttgg ctggagctca attgattaaa actgctttaa ctccacaagt ttttctgacg
    52801 attggtatca tcttaacagc tggtagtatg attgtcactt ggttgggtga gcaaattaca
    52861 gataagggat acggaaacgg tgtttccatg attatctttg ccgggattgt ttcctcaatt
    52921 ccagagatga ttcagggcat ctatgtggac tactttgtga acgtcccaag tagccgtatc
    52981 acttcatcta tcattttcgt aatcattttg attattactg tattgttgat tatttacttt
    53041 acaacttatg ttcaacaagc agaatacaaa attccaatcc aatatactaa ggttgcacaa
    53101 ggtgctccat ctagctctta ccttccgtta aaggtaaacc ctgctggagt tatccctgtt
    53161 atctttgcca gttcgattac tgcagcgcct gcggctattc ttcagttttt gagtgccaca
    53221 ggtcatgatt gggcttgggt aagggtagca caagagatgt tggcaactac ttctccaact
    53281 ggtattgcca tgtatgcttt gttgattatt ctctttacat tcttctatac gtttgtacag
    53341 attaatcctg aaaaagcagc agagagccta caaaagagtg gtgcctatat ccatggagtt
    53401 cgtcctggta aaggtacaga agaatatatg tctaaacttc ttcgtcgtct tgcaactgtt
    53461 ggttccctct tccttggtgt gatttccatt ttaccgattg cagctaaaga tgtatttggt
    53521 ctttctgatg ttgttgcctt tggtggaaca agtctcttga tcattatctc tacaggtatc
    53581 gaaggaatca agcaattgga aggttaccta ttgaaacgta agtatgttgg tttcatggac
    53641 agaacagaat aaaagtattt actgaatcag taaatactga gggagtggag gtttaaactc
    53701 tgacatttgt aagagttgga tctcccctct tctattttgt ttttaaatag gggtgaaaag
    53761 actttttgct tctatttaaa aataaaataa ggagatcaaa tcatgaatct tttgattatg
    53821 ggcttacctg gtgcaggtaa gggaactcaa gcagcaaaaa tcgtagaaca attccatgtt
    53881 gcacatatct caacaggtga tatgttccgc gctgcaatgg caaatcaaac tgaaatgggt
    53941 gttcttgcta agtcatatat tgacaagggt gaattggttc ctgacgaagt tacaaatgga
    54001 atcgtaaaag aacgcctttc acaagatgat attaaagaaa caggattctt attggatggt
    54061 tacccacgta caattgaaca agctcatgcc ttggacaaaa cattggctga acttggcatt
    54121 gaactagaag gtattatcaa tattgaagtg aaccctgaca gcctcttgga acgtttgagt
    54181 ggccgtatca tccaccgcgt aactggagaa actttccaca aggtctttaa cccaccagtt
    54241 gactataaag aagaagatta ctaccaacgt gaagatgata agcctgagac agtaaaacgt
    54301 cgtttggatg ttaatattgc tcaaggagaa ccaatcattg ctcactaccg tgccaaaggt
    54361 ttggttcatg acatcgaagg taatcaagat atcaatgatg tcttctcaga tattgaaaaa
    54421 gtattgacaa atttgaaata aagcgttttt cgcacttgca aaaatccgct acaaatgtta
    54481 tactgagata gtctgactta taattgttgt ctctgtgtct agaggcatcg aatcgaaatt
    54541 tatggaggtg cttttgcgtg gcaaaagacg atgtgattga agttgaaggc aaagtagttg
    54601 atacaatgcc gaatgcaatg tttacggttg aacttgaaaa tggacatcag attttagcaa
    54661 cagtttctgg taaaattcgt aaaaactata ttcgtatttt agcgggagat cgtgttactg
    54721 tcgaaatgag tccatatgac ttgacacgtg gacgtatcac ttaccgcttt aaataatcga
    54781 aaaacttgga gggataagaa atgaaagtaa gaccatcggt caaaccaatt tgcgaatact
    54841 gtaaagttat tcgtcgtaat ggtcgtgtta tggtaatttg cccagcaaat ccaaaacaca
    54901 aacaacgtca aggataagat agaaaggaga aaacatggct cgtattgctg gagttgatat
    54961 tccaaatgac aaacgcgtag taatctcatt gacttatgtt tatggtatcg gacttgcaac
    55021 atctaagaaa attttggctg ctgctggaat ctcagaagat gttcgtgtac gtgatcttac
    55081 atcagatcaa gaagatgcta tccgtcgtga agtggatgca atcaaagttg aaggtgacct
    55141 tcgtcgtgaa gtaaacttga acatcaaacg tttgatggaa atcggttcat accgtggtat
    55201 ccgtcaccgt cgtggacttc ctgtccgtgg acaaaacact aaaaacaacg ctcgcactcg
    55261 taaaggtaaa gctgttgcga ttgctggtaa gaaaaaataa tataggaggt aaaagtcttg
    55321 gctaaaccaa cacgtaaacg tcgtgtgaaa aagaatatcg aatctggtat tgctcatatt
    55381 cacgctacat ttaataacac tattgttatg attactgatg tgcatggtaa tgcaattgct
    55441 tggtcatcag ctggtgctct tggtttcaaa ggttctcgta aatctacacc attcgctgct
    55501 caaatggctt ctgaagctgc tgctaaatct gcacaagaac acggtcttaa atcagttgaa
    55561 gttactgtaa aaggtccagg ttctggtcgt gagtcagcta ttcgtgcgct tgctgccgct
    55621 ggtcttgaag taacagcaat tcgtgatgtg actccagtgc cacacaatgg tgctcgtcct
    55681 ccaaaacgtc gccgtgtata atcatcgcat tacactgctt ttcgtttaag agggagtaac
    55741 taaatgatcg agtttgaaaa accaaatata acaaaaattg atgaaaataa agattatggc
    55801 aagtttgtaa tcgaaccact tgaacgtggc tacggtacaa ctcttggtaa ctctcttcgt
    55861 cgtgtacttc tagcttctct accaggagca gctgtgacat ctatcaacat tgatggtgtg
    55921 ttacatgagt ttgacacagt tccaggtgtt cgtgaagacg tgatgcaaat cattctgaac
    55981 attaaaggaa ttgcagtgaa atcgtacgtt gaagacgaaa aaatcatcga actggatgtt
    56041 gaaggtcctg ctgaagtaac agctggtgac attttgacag atagcgatat tgaaattgta
    56101 aatccagatc attatctctt tacaatcggt gaaggttctt ctctaaaagc gactatgact
    56161 gttaacagtg gtcgtggata tgtacctgct gatgaaaata aaaaggataa tgcaccagtt
    56221 ggaacacttg ctgtagattc tatttataca ccagttacaa aagtcaacta tcaagtggaa
    56281 cctgctcgtg taggtagcaa tgatggtttc gacaaattaa cccttgaaat cttgacaaat
    56341 ggaacaatta ttccagaaga tgctttaggg ctttcagcac gtattttgac agaacatctt
    56401 gatttgttta caaatcttac tgagattgct aagtcaactg aagtgatgaa agaagctgat
    56461 actgaatctg acgaccgtat tttagatcgt acgattgagg aactggactt gtctgtgcgt
    56521 tcatacaact gtttaaaacg tgccggtatc aatactgtgc atgatttgac agaaaaatct
    56581 gaagcagaga tgatgaaagt acgaaatctt ggacgcaaga gtttggaaga agtgaaactc
    56641 aaactcattg atttgggtct tggattaaaa gataaataaa ggaggaatac atggcttacc
    56701 gtaaactagg acgcactagc tcacaacgta aagcaatgct tcgcgatttg acaactgacc
    56761 ttttgatcaa cgaatcaatc gtgacaactg aagctcgtgc taaagaaatc cgtaaaactg
    56821 ttgaaaaaat gattactcta ggtaaacgtg gtgatttgca tgcacgtcgt caagcagctg
    56881 ctttcgtacg taatgaaatc gcatctgaaa actatgatga agcaactgat aagtacactt
    56941 ctactacagc acttcaaaaa ttgttctcag aaatcgcacc tcgttatgct gaacgtaacg
    57001 gtggatacac tcgtatcctt aaaactgaac cacgtcgtgg tgatgcagcg ccaatggcga
    57061 tcatcgaatt agtataaaat catcaatttt gttgagtgtt atgatgatgg agtcttgtgc
    57121 tcttagtcta gctctggtct accgctagga tttcggtcct agcgggaaca ctcatcataa
    57181 gttgggatag tagacgcttg tttacgaaat tgtttttttc ttaagaacaa cttcgtaagc
    57241 aggcgttttt gagtattttc gttagaatta tgctatacta tttgaaaaga atcctgttta
    57301 atgttaaggt ttcttatttt aagaagaatt ggagtttact tatgaaagcc attataactg
    57361 ttgttggtaa agataaatct ggaattgttg caggtgtttc tggtaaaatt gcagaattag
    57421 gattgaatat tgacgatatc tctcaaactg tcttggatga atattttacg atgatggctg
    57481 ttgtatctag tgatgaaaag caagatttta cctatcttcg taatgaattt gaagcttttg
    57541 ggcaaacttt gaatgtaaaa atcaatattc agagtgcagc gattttcgaa gctatgtata
    57601 atatctagga ggttatcatg gatattagac aagttactga aaccatcgcc atgattgagg
    57661 agcaaaattt cgatatcaga accattacca tggggatttc tcttttggat tgtatcgatc
    57721 cagatatcaa tcgtgctgcg gagaaaatct atcaaaaaat tacgacaaag gcggctaatt
    57781 tagtagctgt tggtgatgaa attgcggctg agttgggaat tcctatcgtt aataagcgtg
    57841 tatcggtgac acctatttct ctgattgggg cagcgacaga tgcgacggac tacgtggttc
    57901 tggcaaaagc gcttgataag gctgcgaaag agattggtgt ggactttatt ggtggttttt
    57961 ctgccttagt acaaaaaggt tatcaaaagg gagatgagat tctcatcaat tccattcctc
    58021 gcgctttgac tgagacggat aaggtctgct cgtcagtcaa tatcggctca accaagtctg
    58081 gtattaatat gacggctgtg gcagatatgg gacgaattat caaggaaacg gcaaatcttt
    58141 cagatatggg agcggccaag ttggttgtat tcgctaatgc tgttgaggac aatccattta
    58201 tggcgggtgc ctttcatggt gttggggaag cagatgttat catcaatgtc ggagtttctg
    58261 gtcctggtgt ggtgaaacgt gctttggaaa aagttcgtgg acagagcttt gatgtagtag
    58321 ccgaaacagt taagaaaact gcctttaaaa tcactcgtat cggtcaattg gttggtcaaa
    58381 tggccagtga gagactgggt gtggagtttg gtattgtgga cttgagtttg gcaccaaccc
    58441 ctgcggttgg agactctgtg gcacgtgtcc ttgaggaaat ggggctagaa acagttggca
    58501 cgcatggaac gacagctgcc ttggccctct tgaacgacca agttaaaaag ggtggagtga
    58561 tggcctgtaa tcaggtcggt ggtctatctg gtgcctttat ccctgtttct gaggatgaag
    58621 gaatgattgc tgcagtgcaa aatggctctc ttaatttaga aaaactagaa gctatgacgg
    58681 ctatctgttc tgttggattg gatatgattg ccatcccaga agatacgcct gctgaaacta
    58741 ttgcggctat gattgcggat gaagcagcaa tcggtgttat caacatgaaa acaacagctg
    58801 ttcgtatcat tcccaaagga agagaaggcg atatgattga gtttggtggt ctattaggaa
    58861 ctgcacccgt tatgaaggtt aatggggctt cgtctgtcga cttcatctct cgcggtggac
    58921 aaatcccagc accaattcat agttttaaaa attaagaaaa taggagaaat tttaagttct
    58981 atttaagatt agatgtgtat actataatca ttaaataaag acctcctaat attatttgaa
    59041 acagataaca ctgaattagt ttgaatttga ttttcatcta atatctttat ttaatgaact
    59101 cctaaacttt ttcataataa tctccttcaa aagtcgcctg tatgggtggc ttttatttta
    59161 tcattcatga tataatagaa gcaaacggag gacggaaaat ggtaaaagta cgattgtatt
    59221 tgatacgtca tggcaagacc atgtttaaca cgattggtcg cgcgcaaggt tggagcgata
    59281 ctcccttaac tgctgaaggt gaacgaggga ttcaagagtt aggaattggt ttgcgagaat
    59341 ctgatctaca gtttgagcgc gcttattcga gtgattctgg tcgtaccatt cagaccatgg
    59401 gaattatcct tgaagaactt ggcttgcagg ggaaaatccc ttatcgcatg gacaagcgta
    59461 tcagagaatg gtgtttcggt agttttgatg gagcctatga tggcgatctt ttcatgggca
    59521 ttattcctcg tatctttaat gtggaccacg ttcaccaatt gtcttatgct gaactggctg
    59581 agggcttggt agaggtcgat acagctggtt gggctgaagg ctgggaaaaa ctcagtggcc
    59641 gaatcaagga aggctttgaa atgattgcaa aagaaatgga agatcaaggt ggaggtaacg
    59701 cccttgttgt cagccatgga atgactattg gaaccattgt ttatctgatt aatggcatgc
    59761 atccgcatgg tctagataat ggtagcgtga cgattcttga atatgaggac ggtcagttta
    59821 gggttgaagt tgtcggtgac cgtagttacc gagagctagg acgtgagaag atggaagaag
    59881 gctctattta atcagtctag acttgcttgc catgagctag ggatttgata agaatatcaa
    59941 gataagaaaa aacagccgag ggtactcctt tcggctgttt ttgatgtgga aaactaaagt
    60001 gtaatgctat tgcttttaga gattttcata aacaagagca aggaacctac tgttagaaca
    60061 gtcaggatag ttgacaaggc tgcggctaca ccgtaatttc ctctgagaac ctctgtataa
    60121 atagctacag tcattgttct tgttttgaca ttgtagagga ggatagaagt agagagttct
    60181 gaaatcagtg tgacccaaga taggatggct ccagaaataa tatccgatag catcattgga
    60241 gttgtaatct tagcaaagat attgagacga ctacttccta agctttcagc agcttcttca
    60301 atacttggtg ctatttgttg taagctagca acagatgagc gaatagtata aggtaatttt
    60361 ctggcagata gagacataat caagatgaaa gcagtccctg taatcataag aaatccactt
    60421 ccaaatagac cagagaaatg aaggcaatcc ctagaacggt tcctggtaca atataaggta
    60481 ccatactgag gctgtcaatt aagtttgtaa acaaattccg ttttctaacg gctaggtagg
    60541 agataaatgt cgcaaataga acaactagaa ctaaggcaat caaagggata cgaatggtat
    60601 tgaaaatagc agatcccata cgatggaaag ctaccttgta actgtttgga gaataacctt
    60661 taacagatac catacctgat gtttttagga aagaggtata aattaagtag atttgaggta
    60721 aaacagagat aaagataatt ccgtagactg ttgcataaat ggcagctatt tttccttttg
    60781 tagttttttt aggctcaatt ggatggagca gattcatgct gaaactgtag cggtttgcaa
    60841 tgtgtttttg gataaggaaa attgccaagg caatgataat cgccataatt gcaaaagcag
    60901 aatttcctcc aacctcgcta ataaattggg tataaatcag gacagggaaa gtccgatatc
    60961 cttcgccaat caacataggc gttccaaagt ctgagaatgc tctcataaat acaagcaagg
    61021 agctgctagt aaggttggaa ctaggagagg taaaacaacc gttacgatac gcttaaatcc
    61081 gaaggacccc catgctttca gctgcttcaa gtagagaatt gtcaatactg ttcattgttc
    61141 cagcaacata tagaaatacc agtgggaata gttgcagtgt aaagacaagt acaattcctt
    61201 tgaatccata aatatcgata gctggaagat gaagggcatt tgtcaaaaat ttagtgatga
    61261 ccccatttcg tcctagcaag agaacccagg agtaggctcc tacgaaagga gctgacatgg
    61321 aagcaatgat aatcaatatt tgtagaaatt tctccccctt gaagtcatac atagagaaga
    61381 gataagctaa tagggttcct acaactaagg aagtgacagt agcggtaatg gaaaccttga
    61441 aactgttgac tagtgtctca gagtagtaga ctttactaaa gaaagtgaca aaattagcta
    61501 gtgagaatta tccttcatgt ataagtgctt acttgagcac ggtaatgata ggataaacga
    61561 gaaagacaag ataggtaaga aagatgaaga aagaggaggc tgtccaaata tttagttttt
    61621 tacattccat ggttgactcc ttttatcagg ttttgggaac catctgcaga aaagatgttt
    61681 aatttttgcg tattgattcg tagacgaata cgattgtctt tttgtagatc ttcttcaaaa
    61741 gttgattctt cactaacttg aatttttgag gcaaaacctg tctcaatgaa atactccgta
    61801 tttagtccaa gatagacgct atcgctaata gttccttcaa tatctccaga ttcatctttg
    61861 ataaactctt cgggacgaat gcttacatga atagcttgct cctcaacctg atcaagagct
    61921 ggcattcgaa gggcatagcc atctgaaaag acgatataag cgccgtcgct ccgtttttca
    61981 agattggcag ggataatatt tgtgcgtccg ataaaggttg ccacaaactc attagctggt
    62041 ttatgataga gttcttttgg tcggccgatt tgttggatca ccccatcttt cataacagca
    62101 atttgatctg aaatagccat ggcttcttct tggtcgtggg ttacataaac agttgtaatt
    62161 cccacttcgt gttggatttc tcggatggct tgacgcatat ccaagcgaag tttggcctcc
    62221 agattactaa gtggctcgtc catgaggaga acacttggat taaccgctaa ggcgcatgcc
    62281 aaggtgacac gttgttgttg tccaccactg agtttatcgg gctttcgatc cgcatattga
    62341 gcaatttgca tgagttcaag atacttgttg gtctgttgaa tcaattcttc ttttggaacc
    62401 ttcttttgct taagaccaaa agcaacgttg tctcggacag tcaaatgtgg gaaaatagcg
    62461 tagttttgga aaaccatccc gatattgcgt ttgctgggtt ccatattatt gatttttgta
    62521 tcatcgaagt aaaattctcc atctttgata ctgttgaaac ctgcaatcat acgaagaagg
    62581 gtcgttttcc cacatcctga aggtccaaga agggtaaaga gacttccttt tggaattgta
    62641 atgttcaaat tctcaataac agggacatcg tggtagattt tttggcgtta ataattttga
    62701 tctcactcat agtgaacctt ttttactgtt tagattggat atctgtaaag acttcgttgt
    62761 atttcttaac gatatctgat ttattcttga tgacataatc ataatcttca gtgagtgttt
    62821 tgattttgtc aattggtttc atgttttcgc ttgttttagc atttttacga acaggacggt
    62881 tagtagtggt tgtaccaagt gtatcttgta cttcttgaga gataataaaa tcgataaatt
    62941 tcttgtcatt ttccatattt ttagcttttt taacgatagc agcactagca ggtaggaaga
    63001 cggttccttc ttttggatag actactttaa tgttagctcc gtcatttaag agtttaactg
    63061 ctggatcctc ataagagaga ccaacagcca tttctccatc aacgactgct ttatagacac
    63121 cagatgaact tgaaccgatt ttatagtgta ttgaaactag aatagtacac ctctacttct
    63181 aaaacattgt tagaaatcga tttgactgtc ctgattaatt tgtcctgttc ttatttcatt
    63241 tcactatgcc atcaataagt gtgaaaagat cttttacata agaccaagcc ttatcatctt
    63301 tgtaaccacc ttgagcttgt agcatatttg ttaattgagc aaaggcgcta gaagagtttg
    63361 ctgggtcagc agttgcgatt tttcctttta gttcaggttt gagaaggtcg ctatatcctt
    63421 cgatgttcat gcctttagtt aaatcagggt tgacgattaa aacactacca tctagtgtat
    63481 aaggagtaga gaagccagtt gtgttttgat attctttgat aacattatca ttttcttttg
    63541 aaatatagtt ttcaaagagt tctccgtggg tagcatattg tgtataagaa ccaccaaaga
    63601 taacatcagc tacaggaact tctttttctg actctagttt tttgaaaagt tctccagtac
    63661 cagcttgaat cagttctatt ttgataccat atttttcttc aaaggcagga atagttgctc
    63721 caattaagcc ttctgagttt ggtgaataaa cgactagcga accaccgtct cctttatcag
    63781 atgaactgtc atcggcagat tcattagaag aacaagcagc ataatacatc tatttctttt
    63841 tcatgatgga tatctccgtt gtgttattta agtttatttt aaaacaatgt aagcgttttt
    63901 aaaacctaca attctattct atagtgtatt gaatctataa cagtacactt tgactgctaa
    63961 aatatttcta taaattaatt tgactttcct gatagagatg ttcacatctt atttcaattc
    64021 actatattag agtaaaattc tctacaaaaa gaagaatagc ctatttgact attcttccga
    64081 gtgatttcaa tttctttggg gaaatatgga gatacttttt aaatactttg gcaaaatatt
    64141 tatagtctga aaatccaact tggtcagaga tagcgcttaa aggggagttg ggagattcag
    64201 ccatcttatc tatagcttgt ttcaggcgat attgtaagat atattcattt agagtcatgg
    64261 ggaagtcttc tttgataacc ttatatagat gcttgttatc tgcaaatagc tagagcattt
    64321 caaggaaata aactagaaat aacaattaat atggtggttc agaaatacct tttgataatc
    64381 aataagatct tcaggatgaa gtgcgttgat tccatccatt tcatatttcc gatttaataa
    64441 acgatatttg ttttcaccaa attgatgaaa agggagtagt tgaacttggt cgatatttaa
    64501 tgagttaaat agagtagcga atttttctgc atcctctaaa ctattgttaa aattaggaat
    64561 aactgggatt cttaaaacga tagttttatt ttgagaaaaa g
//
LOCUS       DAVLVF010000004        57399 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVLVF010000004 DAVLVF010000000
VERSION     DAVLVF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 57399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57399
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.004"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(54..803)
                     /locus_tag="VYN66_000204"
     CDS             complement(54..803)
                     /locus_tag="VYN66_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV1494295.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            936..3272
                     /locus_tag="VYN66_000205"
     CDS             936..3272
                     /locus_tag="VYN66_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV1494296.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLMETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDSIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEQNFLRKSMLRALPSALMVVFSVLFVKIF
                     GSSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTGQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            3374..4051
                     /locus_tag="VYN66_000206"
     CDS             3374..4051
                     /locus_tag="VYN66_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV1494297.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            4038..4835
                     /locus_tag="VYN66_000207"
     CDS             4038..4835
                     /locus_tag="VYN66_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV1494298.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4852..5955
                     /locus_tag="VYN66_000208"
     CDS             4852..5955
                     /locus_tag="VYN66_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1494299.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDNLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            6025..6198
                     /locus_tag="VYN66_000209"
     CDS             6025..6198
                     /locus_tag="VYN66_000209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494300.1"
                     /translation="MDRTDEVSSKHCFEVVDRTDEVSSKHCFEVVDRTDEVSNHTHGK
                     ATLTWFELDFRRV"
     gene            6301..7320
                     /gene="galE"
                     /locus_tag="VYN66_000210"
     CDS             6301..7320
                     /gene="galE"
                     /locus_tag="VYN66_000210"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1494301.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            7322..8275
                     /locus_tag="VYN66_000211"
     CDS             7322..8275
                     /locus_tag="VYN66_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1494302.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKIK"
     gene            complement(8337..8543)
                     /locus_tag="VYN66_000212"
     CDS             complement(8337..8543)
                     /locus_tag="VYN66_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV1494303.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            8595..9071
                     /locus_tag="VYN66_000213"
     CDS             8595..9071
                     /locus_tag="VYN66_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV1494304.1"
                     /translation="MAKEPWQEDIYDQEESRAGRRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            9080..9751
                     /locus_tag="VYN66_000214"
     CDS             9080..9751
                     /locus_tag="VYN66_000214"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV1494305.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            9882..10220
                     /locus_tag="VYN66_000215"
     CDS             9882..10220
                     /locus_tag="VYN66_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV1494306.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            10223..10852
                     /locus_tag="VYN66_000216"
     CDS             10223..10852
                     /locus_tag="VYN66_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV1494307.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            11092..12243
                     /locus_tag="VYN66_000217"
     CDS             11092..12243
                     /locus_tag="VYN66_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1494308.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVMPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            12556..13305
                     /gene="truA"
                     /locus_tag="VYN66_000218"
     CDS             12556..13305
                     /gene="truA"
                     /locus_tag="VYN66_000218"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV1494309.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            13295..14068
                     /locus_tag="VYN66_000219"
     CDS             13295..14068
                     /locus_tag="VYN66_000219"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1494310.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            14046..14507
                     /locus_tag="VYN66_000220"
     CDS             14046..14507
                     /locus_tag="VYN66_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1494311.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            14734..16075
                     /locus_tag="VYN66_000221"
                     /pseudo
     CDS             14734..16075
                     /locus_tag="VYN66_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(16203..16406)
                     /locus_tag="VYN66_000222"
     CDS             complement(16203..16406)
                     /locus_tag="VYN66_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1494312.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(16399..17481)
                     /locus_tag="VYN66_000223"
     CDS             complement(16399..17481)
                     /locus_tag="VYN66_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1494313.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(17607..18389)
                     /locus_tag="VYN66_000224"
     CDS             complement(17607..18389)
                     /locus_tag="VYN66_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV1494314.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(18389..19732)
                     /locus_tag="VYN66_000225"
     CDS             complement(18389..19732)
                     /locus_tag="VYN66_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV1494315.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(19831..21147)
                     /locus_tag="VYN66_000226"
     CDS             complement(19831..21147)
                     /locus_tag="VYN66_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV1494316.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            21340..22566
                     /locus_tag="VYN66_000227"
     CDS             21340..22566
                     /locus_tag="VYN66_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV1494317.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            22765..24339
                     /locus_tag="VYN66_000228"
     CDS             22765..24339
                     /locus_tag="VYN66_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1494318.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRIEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            24604..25392
                     /gene="codY"
                     /locus_tag="VYN66_000229"
     CDS             24604..25392
                     /gene="codY"
                     /locus_tag="VYN66_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV1494319.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            25392..25967
                     /locus_tag="VYN66_000230"
     CDS             25392..25967
                     /locus_tag="VYN66_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1494320.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            26049..26134
                     /locus_tag="VYN66_000231"
     tRNA            26049..26134
                     /locus_tag="VYN66_000231"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26083..26085,aa:Leu,seq:aag)
     gene            26417..27547
                     /gene="ugpC"
                     /locus_tag="VYN66_000232"
     CDS             26417..27547
                     /gene="ugpC"
                     /locus_tag="VYN66_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV1494321.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            28048..28806
                     /locus_tag="VYN66_000233"
     CDS             28048..28806
                     /locus_tag="VYN66_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1494322.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            28894..29406
                     /locus_tag="VYN66_000234"
     CDS             28894..29406
                     /locus_tag="VYN66_000234"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV1494323.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            29588..30532
                     /locus_tag="VYN66_000235"
     CDS             29588..30532
                     /locus_tag="VYN66_000235"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV1494324.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            30541..31218
                     /locus_tag="VYN66_000236"
     CDS             30541..31218
                     /locus_tag="VYN66_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV1494325.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            31316..32074
                     /locus_tag="VYN66_000237"
     CDS             31316..32074
                     /locus_tag="VYN66_000237"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV1494326.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            32112..33617
                     /gene="lytC"
                     /locus_tag="VYN66_000238"
     CDS             32112..33617
                     /gene="lytC"
                     /locus_tag="VYN66_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV1494327.1"
                     /translation="MSVTFFIGEQRLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            34115..34633
                     /locus_tag="VYN66_000239"
     CDS             34115..34633
                     /locus_tag="VYN66_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV1494328.1"
                     /translation="MVELKKEAAKDVATLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGHLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLAIYRYLITLFQKALDVTDEEGDDVTNDIFVGAKAELEKTVW
                     MLAAELGQAPGL"
     gene            34763..35269
                     /locus_tag="VYN66_000240"
     CDS             34763..35269
                     /locus_tag="VYN66_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV1494329.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEAYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            35269..35439
                     /locus_tag="VYN66_000241"
     CDS             35269..35439
                     /locus_tag="VYN66_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494330.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            35471..36703
                     /gene="clpX"
                     /locus_tag="VYN66_000242"
     CDS             35471..36703
                     /gene="clpX"
                     /locus_tag="VYN66_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV1494331.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            36712..37299
                     /locus_tag="VYN66_000243"
     CDS             36712..37299
                     /locus_tag="VYN66_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV1494332.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHAPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            37310..37690
                     /locus_tag="VYN66_000244"
     CDS             37310..37690
                     /locus_tag="VYN66_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV1494333.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            37742..38632
                     /gene="rapZ"
                     /locus_tag="VYN66_000245"
     CDS             37742..38632
                     /gene="rapZ"
                     /locus_tag="VYN66_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV1494334.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            38629..39606
                     /locus_tag="VYN66_000246"
     CDS             38629..39606
                     /locus_tag="VYN66_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV1494335.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            39603..40514
                     /gene="whiA"
                     /locus_tag="VYN66_000247"
     CDS             39603..40514
                     /gene="whiA"
                     /locus_tag="VYN66_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV1494336.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(40548..41516)
                     /locus_tag="VYN66_000248"
     CDS             complement(40548..41516)
                     /locus_tag="VYN66_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1494337.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEDYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQTLQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            41652..42509
                     /locus_tag="VYN66_000249"
     CDS             41652..42509
                     /locus_tag="VYN66_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV1494338.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYMSTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            42496..43275
                     /locus_tag="VYN66_000250"
     CDS             42496..43275
                     /locus_tag="VYN66_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV1494339.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            43299..44651
                     /locus_tag="VYN66_000251"
     CDS             43299..44651
                     /locus_tag="VYN66_000251"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV1494340.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            44725..45099
                     /locus_tag="VYN66_000252"
     CDS             44725..45099
                     /locus_tag="VYN66_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV1494341.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPNELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            45101..45949
                     /locus_tag="VYN66_000253"
     CDS             45101..45949
                     /locus_tag="VYN66_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1494342.1"
                     /translation="MKLAVFTDSSAYLSAEILQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            46226..46993
                     /locus_tag="VYN66_000254"
     CDS             46226..46993
                     /locus_tag="VYN66_000254"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV1494343.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            47005..48189
                     /locus_tag="VYN66_000255"
     CDS             47005..48189
                     /locus_tag="VYN66_000255"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV1494344.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDLDLLLQAENLRQAQGKEVNPQVIKETYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            48186..50057
                     /locus_tag="VYN66_000256"
     CDS             48186..50057
                     /locus_tag="VYN66_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1494345.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQN
                     FNLLVQAQDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(50200..51384)
                     /gene="mntE"
                     /locus_tag="VYN66_000257"
     CDS             complement(50200..51384)
                     /gene="mntE"
                     /locus_tag="VYN66_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV1494346.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            51688..54384
                     /locus_tag="VYN66_000258"
     CDS             51688..54384
                     /locus_tag="VYN66_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV1494347.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            54397..55188
                     /gene="yghU"
                     /locus_tag="VYN66_000259"
     CDS             54397..55188
                     /gene="yghU"
                     /locus_tag="VYN66_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV1494348.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLRTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            55304..55714
                     /locus_tag="VYN66_000260"
     CDS             55304..55714
                     /locus_tag="VYN66_000260"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1494349.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            55726..57321
                     /locus_tag="VYN66_000261"
     CDS             55726..57321
                     /locus_tag="VYN66_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494350.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGRTFEGQVGKDSYLKTFKVQ"
BASE COUNT        16661 a        10814 c        13182 g        16742 t
ORIGIN      
        1 ccaatctact atataaatga aaaccttagc tgtttggcca aggttcattt cttctatgca
       61 agttcttctc ttttcttatc tgggttccag atgaagcttg cgataaagct aaagatgatg
      121 gttaggatag cgaggataat agcaaggatg agggcaggga tgcggataaa ccaccagagt
      181 ggatttggtt ttttatcatt gtgccattgt ttcgtttctt cgtccagacg ttggaattct
      241 gtttgaatac gattggcgac tgtttcaatg ccttcatcat tcattttctt gatatctgag
      301 atatcgattg gatttccaaa gttcatatcg acacgttcac ggctaatcaa gcccttcaaa
      361 gtcatgggac cggtgtaggt aaccggcatg atacggacct tggccatttt ggcaatcagt
      421 gctgcgcccc ccttgacatc gtttgagtgg cggctaccac ttggaaacat gatgagagag
      481 cggtcacttt ttttgagaac gttgatagga tatttgatgg ctgaggcgct gggattttca
      541 cggtcgatgg gaaaggcgcc acacatacga atccaccaac caaagatacg gttggtaaag
      601 agttcttttt ttgccataaa gatgaactgt tttggcttgg tcgcaaaggc catataaaca
      661 ggatcccacc aggtacggtg aggcgcaact aaaatataat tttcatcttg attaggaatt
      721 ttatcagtat tatgatagtg agcattgcca ttgatggacc ataagagcaa tacaactaat
      781 ccacgcaaat aagtataaaa catgcgatct ccttcgattg ttttcttgtt attattatac
      841 cttatcaaag gagggctggc aaacttttcc cttgactaga tacatattta ggatgaaatt
      901 agaattctgt taaaaaaaat gatataatag aatttatgga taaaaataag attatgggat
      961 taacccaaag agaagtcaag gaaagacagg ctgagggttt ggtcaatgac tttaccgcat
     1021 cagccagtac cagcacttgg caaatcgtta aacgaaatgt ctttaccctt tttaacgctt
     1081 tgaactttgc cattgctttg gctcttgcct ttgtgcaggc ttggagcaat ctggtcttct
     1141 ttgctgttat ctgctttaac gctttttctg ggattgtgac cgagctacga gccaaacaca
     1201 tggtggacaa gctcaatctc atgaccaagg aaaaggtcaa aaccatccgt gatggtcagg
     1261 aagttgctct taatcctgaa gaattagtgc taggagatgt cattcgtttg tctgcaggag
     1321 agcagattcc tagtgatgcc ttggttttgg aaggctttgc ggaagtcaat gaagccatgt
     1381 taacgggaga aagtgatttg gtgcaaaagg aagttgacgg cttacttttg tcaggaagtt
     1441 tcctagccag tgggtcagtt ttatctcaag ttcaccatgt cggtgcagac aactatgctg
     1501 ccaaactcat gcttgaggct aagaccgtta aacccatcaa ctcccgtatc atgaaatcgc
     1561 tggacaagtt ggctggtttt actgggaaga ttatcattcc ctttggtctg gctctcttgc
     1621 tggaagcctt gcttttaaaa ggcctgcctc tcaagtcatc cgttgtaaac tcgtcgacag
     1681 ctcttttggg aatgttgcct aagggaattg cccttttgac cattacttcg ctcttgactg
     1741 cagtgattaa gttgggcttg aaaaaggtct tggtgcagga gatgtactct gttgagacct
     1801 tggcgcgcgt ggatatgctc tgtctggaca agacgggtac catcacccaa ggaaagatgc
     1861 aggtggaggc tgttcttccg ttgatggaaa cgtatggtga agaggctatt gccagcatct
     1921 tgactagcta catggcccat agtgaggata agaatccaac tgcccaagcc attcgccagc
     1981 gttttgtggg agatgttgct tatcctatga tttccaatct tcccttctcg agcgaccgca
     2041 agtggggggc tatggagtta gaaggcttgg ggacagtttt cttaggggcg cctgagatgt
     2101 tgctggattc tgaagtccca gaagccaggg aggccttgga gagaggatca cgtgtcttgg
     2161 tcttagctct cagtcaggag aaattagacc atcacaaacc acagaaacca tctgatattc
     2221 aggctctagc cttgctggaa atcttggact ccattcgaga gggagcagca gagacgctgg
     2281 actatctccg ttctcaggag gtaggactca agattatctc tggtgacaat ccagttacgg
     2341 tgtccagcat tgcccagaag gctggttttg cggactatca caactatgta gattgctcaa
     2401 aaatcaccga tgaggaattg atggccatgg cggaggagac agctattttc ggacgtgttt
     2461 cccctcatca aaagaaactc atcatccaaa cgttgaaaaa agcgggtcat acaacggcta
     2521 tgacagggga cggggttaat gatatcttgg cccttcgtga ggcggattgt tctatcgtga
     2581 tggcggaggg ggatccagca acccgtcaga ttgccaatct ggttctcttg aactcagact
     2641 ttaatgatgt tcctgagatt ctcttcgagg gtcgtcgcgt ggtcaataac attgcccaca
     2701 tcgccccgat tttcttgata aagaccatct attccttcct gttagcagtc atctgtattg
     2761 ccagtgcttt actaggtcgg tcagagtgga ttttgatttt ccccttcatt ccgatccaga
     2821 ttaccatgat tgaccagttt gtggaaggtt tcccaccatt cgttctgact tttgagcgaa
     2881 atatcaaacc tgttgagcag aatttcctca gaaaatccat gcttcgtgcc ctaccaagcg
     2941 ctctcatggt cgtctttagc gtcctgtttg tgaaaatatt tgggagtagc caaggttggt
     3001 ctgagttaga aatctcaact ctcctctatt atctcttggg gtcaattggt ttcttatccg
     3061 tatttagagc ctgcatgcca tttaccctat ggcgtgtcct cttgattgtt tggtcagtag
     3121 gaggtttcct agccacagct ctcttcccaa gaatccaaaa actgcttgaa atttcaacct
     3181 taacaggaca aacattacct gtttatggtg tcatgatgtt ggtctttacc gtgattttca
     3241 tcctgaccag tcgctatcaa gccagaaaat aaagaaaggc tgcaatctgt ggattgcagt
     3301 ttttttaggt acaagattgc cagtcgaaat gtggtataat aagaggtaat agagttttgg
     3361 aaagtgagag aagatgattt caaagagatt agaattggta gcttcctttg tgtcacaggg
     3421 ggctatttta ctagatgtgg gaagtgacca tgcttatctg cctatcgagt tggttgagag
     3481 aggccaaatc aaaagcgcta ttgcaggtga ggtggtggaa ggtccctatc agtctgcggt
     3541 taaaaatgtt gaggctcacg gcctaaagga gaaaatccaa gtccgtttag ccaatggctt
     3601 ggcagctttt gaagagactg accaagtgtc tgtcattacc attgctggca tgggtggtcg
     3661 tttgattgct aggattttag aagaaggttt ggggaagtta gctaatgtag agcgtttgat
     3721 cctccagccc aataatcgtg aagacgactt gcgtatctgg ctacaggatc atggattcca
     3781 gattgtagca gaaagcattt tagaagaagc tggaaagttt tatgagattt tggtggtgga
     3841 agcaggacaa atgaagctat cagccagtga tgttcgcttt ggtcccttct tgtccaaaga
     3901 agtcagtcca gtatttgtcc aaaaatggca aaaagaagct gagaagctag agttcgccct
     3961 cggacaaatc ccagaaaaaa atctggaaga acgtcaagtt ctagtagata agattcaagc
     4021 tatcaaggag gtgctccatg ttagcaagtg aagtgatcca agcttatgaa gccttttgcc
     4081 ctcaggaatt ttctatggag ggagacagcc gtggtctgca aattggcact ttagacaagg
     4141 gtatccaaag ggtcatggtt gccctcgata ttcgtgaaga gacggtggca gaggctattg
     4201 aaaagggtgt ggacttgatt atcgtcaagc acgcgcctat ctttcgtcct atcaaggact
     4261 tgcttgccag ccgtccgcaa aatcagattt acatcgacct gattaagcat gatatcgcag
     4321 tttatgtcag ccataccaat attgatatcg ttgaaaatgg gctcaatgac tggttttgtc
     4381 agatgctagg aatcgaggag acgacttatc tgcaggaaac aggtccagaa cgtggaattg
     4441 gacgtattgg gaatattcag cctcagacat tttgggaatt ggcccaacag gtcaagcaag
     4501 tctttgacct agatagcctt cgaatggtgc attatcaaga gaatgatttg cagaagccta
     4561 tttcaagagt agcaatttgt ggtggaagcg ggcagtcttt ctataaggat gctttggcaa
     4621 agggggcaga tgtctatatc actggtgata tctactacca tactgctcag gatatgctgt
     4681 ctgatggctt gttagcattg gatccaggtc actatatcga agtgcttttt gtggaaaaaa
     4741 tcgcagcact ccttactcaa tggaaagaga aaaaaggctg ggaactagag attttaccta
     4801 gtcaagcatc gaccaatcct ttccgtcata tctagttaga aagtgaagac aatgaaaaaa
     4861 gttgccatta ttggagcagg aattgtggga gcaacagctg cctactacct ctcgagagaa
     4921 agtgacctag aggtgaccgt ttttgaccat gagcaaggtc aagccaccaa ggccgcagca
     4981 ggaattatca gtccttggtt ttccaaacgc cgtaataaag cctggtacaa gatggcgcgc
     5041 ttgggggctg atttttatgt ggatttatta gctgatttag agaaatcagg acaagaaatc
     5101 gacttttacc agcgttcggg agtctttctc ttgaaaaagg atgaatccaa gttggaagaa
     5161 ctctatcaac tggctctcca gcgcagagaa gaatctcccc tgataggcca gttagccatt
     5221 ctggatcaag catcagctaa tgaattattc cctggcttgc agggattcga caacttgctc
     5281 tatgcttctg gtggagcgag agtagatggc caactcttgg taactcgtct gctagaagcc
     5341 agtcatgtca agttggtcaa agaaaaagtg actctgacac tcttatcatc aggctaccag
     5401 attggcgaag aggtgtttga tcaggttatt ttggcaacgg gagcttggtt gggggacatg
     5461 ttagaacctt taggttatga agtggatgtc cgtcctcaaa aaggacaact acgagattat
     5521 cagcttgccc aagacatgga agattaccct gttgtcatgc cagaagggga gtgggatttg
     5581 attccctttg caggtgggaa attatccttg ggcgctaccc atgaaaatga catgggattt
     5641 gatttgacgg tagatgaacc cttgctccaa caaatggagg aggcggcctt gcctcattac
     5701 ccagttttag ctagagtgac ttcaagggct gagcgtgtgg gaatccgtgc ctataccagt
     5761 gatttctctc ccttctttgg gcaggtgcca gaattggcag gtgtctatgc agccagtgga
     5821 ctaggttcat caggcctcac aactggtcct atcattggtt accatctagc ccaactgatc
     5881 caagacaagg agttgacctt ggaccctcta aattacccaa ttgaaaacta tgtcaaacga
     5941 gtaaaaagcg aataatactc aatgaaaatc aaagaacaaa ctaggtaact agccgcgggt
     6001 tactcaaagc actgctttga ggttgtggat agaactgacg aagtcagctc aaaacactgt
     6061 tttgaggttg tggatagaac tgacgaagtc agctcaaaac actgttttga ggttgtggat
     6121 agaactgacg aagtcagtaa ccatacccat ggcaaggcga cgttgacgtg gtttgaattg
     6181 gattttcgaa gagtataagc attttactga aattttatca ggtagtttag gatggcaaat
     6241 gacattccct gtcaaaaatg gtaaaataag aaaaaataat ctgagaatcg aggaaaaaag
     6301 atgcaagaaa agattttggt aactggtggt gccggtttta ttggaaccca cactgttatt
     6361 gaattgatcc aagcaggcca tcaagttgtt gtggtggata atcttgtcaa tagcaatcgt
     6421 aagagtttag aagttgttga aagaatcaca ggagttgaga ttcctttcta tgaggcagat
     6481 attcgtgaca cagctaccct cagagatatt ttcaagcagg aagaaccgac tggtgtcatt
     6541 cactttgctg gtttgaaggc tgttggcgaa tcaacacgta tccctcttgc ctactatgac
     6601 aacaatatcg ctggaactgt cagccttttg aaagccatgg aagaaaacaa ctgtaaaaac
     6661 atcatcttca gttcttctgc gacagtttac ggggatccgc acacagtgcc catcttggaa
     6721 gatttcccac tttcagtgac caacccatac ggtcgtacta agctcatgct agaggaaatt
     6781 ttgactgata tttacaaagc agactcagaa tggaatgttg tcttgcttcg ttactttaac
     6841 ccaatcggag cccatgagag tggtgatttg ggagaaaatc caaacggtat tccaaacaat
     6901 ctcttgccat atgtgactca agtagccgtt ggaaaattag agcaagtgca agtgtttgga
     6961 gacgattacg atacggaaga tggaacaggt gttcgtgact atatccacgt tgtcgatttg
     7021 gctaagggtc acgttgcagc tttgaaaaaa atccaaaaag gttcaggact aaacgtttat
     7081 aaccttggaa ctggtaaagg ttactcagtt cttgaaatta tccaaaacat ggaaaaagcg
     7141 gtgggccgtc ctattcctta ccgcatcgta gaacgtcgcc caggtgatat cgctgcctgc
     7201 tactcagacc cggcaaaagc taaagcagaa ctcggttggg aagcagaact cgacatcacc
     7261 caaatgtgtg aagacgcatg gcgttggcaa agcaagcatc caaatggatt tgaagactaa
     7321 gatgatgatt tcaatcatcg tcccttgttt aaacgaagag gaagtacttc ctctttttta
     7381 tcaggctctg gaagctttac ttccagattt ggaaacagaa atcgagtatg tctttgtcga
     7441 tgatggatca agtgatggga ccttggaact cttaaaggcc tatcgggagc aaaatccggc
     7501 agtccattat atttctttct ctcgaaattt tggcaaagaa gcagcccttt atgcaggctt
     7561 gcaatatgcg acaggagatt tggtggtggt gatggatgca gacctccaag atcctcctag
     7621 tatgttgttt gagatgaaaa atgtactaga caaaaatgta gacttggact gcgttgggac
     7681 acggagaact agtcgggagg gagaaccctt ctttcgcagt ttctgtgctg ttctctttta
     7741 tcgcctcatg caaaaaatca gcccagtagc tctgccgtcg ggtgtccgtg attttcgtat
     7801 gatgagaagg tctgtagtcg atgccatttt aagcttgacc gagtccaatc gtttttcaaa
     7861 ggggctcttc gcctgggtgg gtttcaaaac ctactatttg gactatccaa atgtcgaaag
     7921 gcaggctggc aagaccagtt ggagttttag gcaactcttt ttttactcca ttgaagggat
     7981 tgttaatttt tcagatttcc ctttgactat agcctttgta gctggtctcc tatcttgttt
     8041 tctttctctg ctgatgacct tttttgttgt ggttcggacc ctcattttgg gcaatccgac
     8101 atctggttgg acctctctga tggctgttat tctctttctt ggaggcattc aactcttgac
     8161 cattgggatt ctcggtaagt atatcagtaa gatttattta gagactaaaa aaagaccact
     8221 ttatcttatc aaagaaaaaa gtgaccttcc tgattttaca gaaaaaataa agtgaaaaga
     8281 ctataatttt acatggaaat gtgctaaact agagggagtg gattatcgcc attgatttag
     8341 gcttcctggt ttccaatcag ggcgcgagtt gggcaatttt taacagcctc taagacatcc
     8401 tgactaggag aaatttcttt ttccagttgg tcagggtcat cgtaaaaacg cacgattcca
     8461 ttatcgtggt aatcaaataa atcagaataa gtttggcaaa gtccacaggc gatacatcgt
     8521 tcaggtataa gtgtgatttt catgttcata tttatattgt aataagaaaa gtaaaaaaaa
     8581 acaaggagta aggtatggca aaagaaccgt ggcaagaaga tatttatgat caagaagaat
     8641 caagagcagg gcgtcggcat cgaaaccacg gaggggctga taggatggct aatcgtattt
     8701 tgacgatcct agctagtatt ttctttgtaa ttgtggtggt gatggtcatc gttctcatct
     8761 atctatcatc gggggggagt aatcgcacag cagccttaaa agactttcat gattctgatg
     8821 caagtgtagt acaaatctca tcttcaagca gttctcagcc tgagcagagt tcagagccag
     8881 aatctacttc tagtagttca gaagaagctg ctaatcctga aggaacgatt aaagttctcg
     8941 caggagaagg ggaagcagct attgccgctc gtgcaggaat ctccattgct cagttagagg
     9001 ccttgaatcc tgggcacatg gctacaggat cttggtttgc taatccaggt gatgttataa
     9061 aaataaaata ggagccgata tgaaaacaat tcaaattgct attgatggtc ctgcttccag
     9121 cggtaagagt acggtcgcaa agattattgc taaggatttt gggttcacct accttgatac
     9181 aggagctatg tatcgtgcag cgacctatat ggctcttaag aaccaattag gagttgaaga
     9241 agtcgaagcc cttctagcct tgttggacca gcatccaatc agctttggac gttcagaaac
     9301 tggagaccag cttgtttttg taggagatgt ggatattacc catcctatcc gtgaaaatga
     9361 agtgaccaat catgtttctg ctattgcagc aattcctgaa gtgcgtgaga aactggtttc
     9421 tctccaacaa gaaattgccc agcaaggcgg gattgtcatg gatggtcgcg atattggaac
     9481 tgttgtattg ccacaagcag aattgaaaat tttcctagta gcttctgttg atgagagagc
     9541 agagcgtcgt tacaaggaaa atattgccaa gggaattgaa acagaccttg aaaccctaaa
     9601 aaaggaaatt gctgcgcgtg actacaagga tagtcatcgt gagacttctc ctctcaaaca
     9661 agcagaggat gctgtctacc ttgatacaac tggtttgaac attcaagaag tagttgaaaa
     9721 aatcaaagca gaagctgaaa aaagaatgta aatgtagaaa agccgataga ttgatatcgg
     9781 tttttttcta aatttgtcaa aatgctgatt ttagggtata atagttgctg ttcgagaaat
     9841 tttgattttc aaagaatagt gaatgatgat aaggagaaac catgaacaat ttaccaaatt
     9901 gtccaaaatg taactcagag tatgtctacg aagacggtgc cctactggtt tgcccagagt
     9961 gtgctcatga gtggaatcct gctgaagttg cagaagtaga agaaggtctt gtcgctatcg
    10021 atgccaacgg aaataaattg actgatggtg atacagtaac tctcatcaag gacttgaaag
    10081 taaaaggtgc gccaaaagat ttgaaacaag ggacgcgcgt gaaaaatatc cgcatcgtag
    10141 aaggcgatca caatatcgac tgtaagattg atggttttgg tgccatgaaa cttaaatcag
    10201 agtttgtgag gaagatttaa gcatgtcaaa acactataaa cttgtatttt atagccgtat
    10261 cttcttgttt ctagcggctt ttacgggagt ttatcttgaa attactaggc atggtggttt
    10321 tgggatgctt ctctattaca cggttctgtc caacctcttg gtaactattt ttacccttta
    10381 tcttctaaag gttatgagcc gtgtaggtga aaattggcaa aggccaagtc tcttgcgctt
    10441 aaaaggtggg gtcaccatga gtatcatgat tacctgtgtg atttaccatt tcctcttggc
    10501 gcccattgcg actaatttct ataccctaga aaatttcctt tgccactata tcgttcccat
    10561 ctggttttta gcggataccc tcttttttga caaacagggt caatacaaga tttgggatcc
    10621 agcagtgtgg accattttac cctttctgta tatgatgttt gctcttttta atggcttggt
    10681 tctaaaactc aatattccaa atgccaagga taatcctttc ccttacttct ttttgaatgt
    10741 gaacaagggt tggaatgttg tgtttaagtg gtgtctgatt atctttgttg cctatatggt
    10801 agcaggattt attttctact ttatcaagca aatcaagaga aagtcatcct aatactcttc
    10861 gaaaatctct tcaaaccacg ttagtttcca tctgcaacct caaaacactg ttttgaacaa
    10921 cctgcggcta gctttctagt ttgctctttg attttcattg agtatgagat acaagggatt
    10981 cattttcgaa tttaagaagg agtctacaaa ccaatgaggc ttcttctttt cttgcttatt
    11041 tgataactat caaaaaagta gaagattaag agaaatatag aaagagattt catgaaacaa
    11101 tttttagaac gggccagcat tttggctctc tccctcgttt tgattacctc cttttccatt
    11161 tcgagtgccc taccagccat gtttgactat tatcagggtt attctaagga acaaattgag
    11221 ctcttggtga gcttgccttc ctttggaatc atgatgatgt tactgctaaa tggtttctta
    11281 gaaaaaatat ttcctgagcg cttacagatt agtttgggct tgctgatttt atcattgagc
    11341 ggtacagctc ccttctggta ccaagcctat ccctttgtct ttggaacacg gcttctcttt
    11401 ggtttgggtc ttgggatgat caatgccaag gccatttcta ttatcagtga acgctaccaa
    11461 ggaaaaaggc gaattcagat gttagggcta cgcgcttctg cagaggtcgt tggagcttct
    11521 ctcattacct tggccgtcgg tcagttgttg gcctttggtt ggacagctat ctttctagcc
    11581 tatagtgctg gatttttggt gatgcccctt tatctgctct ttgtccctta tggaaaatca
    11641 aagaaagaag tcaagaaaag agcgaaggaa gcaagtcgtt taactcgaga aatgaaaggc
    11701 ttgattttta ccttagctat cgaagcggca gttgtagttt gtaccaatac agctattacc
    11761 atccgtattc caagtttgat ggtggaaaga ggattggggg atgcccagtt atctagtttt
    11821 gttcttagta tcatgcagtt gatcgggatt gtggctgggg tgagtttttc tttcttgatt
    11881 tctatcttta aagagaaact gctcctctgg tctggtatta cctttggctt ggggcaaatc
    11941 gtgattgcct tgtcttcatc cttgtgggtg gtagtagcag gaagtgttct ggctggattt
    12001 gcctatagtg tagtcttgac gacggtcttt caacttgtct ctgaacgaat tccagctaaa
    12061 ctcctcaatc aagcaacttc atttgctgta ttaggctgta gtttcggagc ctttacgacc
    12121 ccatttgttc taggtgcaat tggcttacta actcacaatg ggatgttggt ctttagtatc
    12181 ttaggaggtt ggttgattgt aatctctatc tttgtcatgt acctacttca gaagagagct
    12241 taggattgat tcctaagttt ttcttttgat actcaatgaa aatcaaagag catagttgat
    12301 tgagtttgga atagtacgct gtagctgcta aaatatttct agaaattaat ttgacttttc
    12361 taatagagtt attcatatct tatttcaatc tactatagaa agctagccgc aggttgctca
    12421 aaatagtgtt ttgaggttgc agatggaaac tgacgtggtt tgaagagatt ttcgaagagt
    12481 atgagatttt tatacccata caatatttgt tttaggtcct tgtttacaga ctaatttctt
    12541 gataaaatag aggttatgac gagatataaa gcaactattt cctatgatgg ttatgccttt
    12601 gctggctttc agcgccagtc tcatgcgcgt agcgttcagg aagaaattga aaaaaccttg
    12661 accagattaa ataaagggca aaccattact gttcacggtg ctggtaggac agatagtggg
    12721 gttcatgccc tgggacaggt cattcatttt gacctgcctt atcagatgga tgaggagaaa
    12781 ctccgttttg ccttggatac ccagtctcct gaagatattg atgtgatttc gattgagctt
    12841 gtggcagatg attttcattg ccgttatgcc aaacatagca agacctatga gtttattgtg
    12901 gatagaggac gtcccaaaaa tcctatgcgc cgtcactatg ccacccactt tccctaccca
    12961 ctcgatgtgg aacgaatgca gattgcaatc aaaaagctag agggaaccca tgattttacc
    13021 ggttttacag cctctgggac tagtgtagag gataaggttc gcaccatcac agaagctagt
    13081 ttaatagtcg atgagacagg acaatttttg acctttacct tttcaggaaa tggtttcttg
    13141 tataaacaga ttcgcaatat ggtggggact cttctcaaaa tcggtaacaa ccgcatgcca
    13201 gtagagcaga ttgatctcat cttggagaag aaggacaggc aacttgcagg tcccactgca
    13261 gcaccaaatg gtttgtattt aaaggagatt cgttatgaag aataatcgta ttttagcact
    13321 ttctggaaat gatattttta gtggtggtgg actgtcagct gatttggcta cctatacctt
    13381 gaacggcttg catgggtttg tagcagtgac ttgtttgaca gccttgacag aaaaaggatt
    13441 tgaagtcttt ccaactgatg ataccatttt tcaacatgaa ttagatagct tacgtgatgt
    13501 ggaatttggg ggaattaaga ttggtcttct ccctactgtc agtgtggctg agaaggcctt
    13561 ggactttatc aaacaacgcc caggagtacc tgtggtgttg gatcctgtct tggtctgcaa
    13621 ggaaacgcat gatgtagctg tcagtgagct ctgccaagag ttgattcgct ttttccctta
    13681 tgtcagtgtg attacgccta atctcccaga agcagaatta ttatccggtc aggaaattaa
    13741 aaccttggaa gacatgaaaa ctgcagcgca gaaattgcat gatttaggag cgccagcagt
    13801 cattatcaag ggaggcaatc gtcttagtca ggacaaggct gtggatgtct tttatgatgg
    13861 acagaccttt actatcctag aaaatccagt tatccaaggc caaaatgctg gtgcaggttg
    13921 tacctttgcc tctagcattg ccagtcactt gattaaaggt gataaacttt tgccagcagt
    13981 agaaagctct aaggctttcg tttatcgtgc tattgcacaa gcagatcagt atggagtaag
    14041 acaatatgaa gcaaacaaaa acaactaaaa tcgcccttgt atccctatta accgcccttt
    14101 ctgtggttct aggttatttc ttaaaaatcc caacacctac aggaattcta actcttttag
    14161 atgctggtgt cttctttgcg gccttttact ttggtagtcg tgaaggagcg gtagtcggag
    14221 gactagcaag tttcttgatt gacctcttat caggctaccc tcagtggatg ttctttagct
    14281 tggtcaacca tggcttgcag ggatttttcg caggatttaa aggaaaaagt cagtggttag
    14341 gccttatttt agcaactatt gccatggtag gaggctacgc cttgggttct actttgatga
    14401 atggctgggc agcagccctc ccagaaattc taccaaattt catgcaaaat atggtaggga
    14461 tgattgtagg atttattctt agtcaaagta tcaagaagat taagtaatac tcttcgaaaa
    14521 tctcttcaaa ccacgtcagc ttccatctgc aacctcaaaa cactgttttg agcagcctga
    14581 ggctagcttt ctagtttgca ctttgatttt cattgagtat aaagaggttg agtaaaaagt
    14641 atttcaaata agaaaaacgc atagtatcag gtgttgaatg aactgccccc caaaagttag
    14701 acagaaaaaa tctaactttt ggggtgtttt attatgaaac taacttatga tgataaagtt
    14761 cagatctatg aacttagaaa acaaggatat agcttagaga agctttcaaa taaatttggg
    14821 ataaacaatt ctaatcttag gtacatgatt aaattgattg atcgttacgg aatagagttc
    14881 gtcaaaaaag ggaaaaatcg ttactattct cctgatttaa aacaagaaat gattcataaa
    14941 gtctgacatg aaggctagac taaagataga gtttctcttg aatacggtct cccaagtcgt
    15001 acgatacttc ttaactggct agcacaatac aggaaaaacg ggtatactat tgttgagaaa
    15061 acaagaggga gagtacctaa aatgggacgt aagccaaaaa agagacctga agagaggaca
    15121 gaattagaac gtcttcaagc agaaaatgag tacctgagag cggagaatgc catcctaaaa
    15181 aagttaagag aactccgatt gaaggaggaa aaagagaaag acagaaattg tttaagaatt
    15241 aatgactgag ttttcgttag atcttcttct aaaagccatt aaactagctc gttcgaccta
    15301 ctactatcac ttgaaacagc tagacaaaac agataaggac caagagctta aagctgaaat
    15361 tcaatccatt tttatcgaac acaagggaaa ttatgcttat cgtcgaattc atttagaact
    15421 aagaaatcgt ggttatctgg taaatcataa aagagttcaa ggcttgatga aagtactcaa
    15481 tttacaagct aaaatgcgac agaaacgaaa atattcttct cataaaggag acgttggtaa
    15541 gaaagcagag aatctcattc aaggccaatt tgaaggctct aaaacaatgg aacagtgcta
    15601 cacagatgtg actgaatttg ccattccagc aagtactcaa aagctttact tatcaccagt
    15661 tttagatggc tttaatagcg aaattatcgc ctataatctt tcaacttcac ccaacttaga
    15721 acaagtacaa acaatgttgg aacaggcatt cacagagaag cactacgaga atacgattct
    15781 ccatagtgac caaggctggc aatatcaaca cgattcttat catcagtttc ttgagggtaa
    15841 gggaattcaa gcatccatgt cacgcaaggg aaacagccaa gacaacggta tgatggagtc
    15901 cttctttggg attctgaaat cggaaatgtt ttacggttat gagaagtcgt ttcagtcgct
    15961 taagcaattg gaacaagcta ttgtagacta tattgattac tacaacaata aacgaattaa
    16021 ggtaaaacta aaaggactta gtcctgtgca atacagaact aaatccttcg gataaattaa
    16081 ttgtctaact gtttgggggc agtacatttt aacacttgat actatgcgtt ttattgtggg
    16141 aagattttcc tcattttcta gtgaaattga actttttcta acatcttttg atagatgact
    16201 tcttaatcaa ttatcatttc cattgttgtt tcttggggtt tcatcccttg tctttgataa
    16261 aagcggagag caccctcgtt atcattccag acacttaggg ttagattatt gcagccaatt
    16321 tctttagcat aatagcagaa aaatgacaat aaaatagccc ctagatattt tgaaaggaaa
    16381 gcataggggc tatatagttt aatcaaaata aagcaaatct ttgctggtgc ttgtaaagag
    16441 gtcgaagcca tccttggtaa caacaccgca gtcttcaata cgaacaccga ctttaccagg
    16501 gatatagata cctggttcaa cagagaagca catgccttct tcgatgacca tgtcgtttcc
    16561 ttccatgata gatgggaatt catggacatc cataccgata ccatgcccga gacggtggtt
    16621 gaagtactca ccataaccag ctttttcgat gacctcacgg gcagcgcggt ccacttcatg
    16681 agcagtcaca cctggcttga taaagtcaag agcagcttgt tgggcttcaa gagtcaagtt
    16741 gtaaatatct ttcttgaatt ggtctggttt gccgacagcg actgtacgag tcatatctga
    16801 cgcatagcca ttgaccagaa cacccaggtc aaagaggaga agagcatcat tttcaacctt
    16861 attagctgct ggaatgccgt gtggattcgc agcattatca ccagtcaaga ccatggtatc
    16921 aaagctcatt tcataacctt cacgtttcat ggcaaagtcg atttgtgcga tgatatctgt
    16981 ctcagtctta tcaagagaaa tattgtcaaa accaacatga acagccttgt cagcataaag
    17041 acctgcaacc atcatttttt gcacttcatc agctgatttg atgaggcgca tgcgttggat
    17101 acgaggagtg aggttgtcaa actcagcagt ctcaaaaact gttttcaaac catggtattt
    17161 ggtcaagatg agattgtcaa actcaacagc gacacgtttg aagtcaagtt gtggaagagc
    17221 atgtttgatt ttttgccatg gattttcaga atcgacatag cccactactg ggaaggaaac
    17281 ggtgctactt gcacgttcta cttcaagagc tgggacaaag aggagaggtt cctgatccgc
    17341 taggacaaag aggaacattt ggcgttcatg gggatcactg taaaaaccag tgaggtaatt
    17401 gattgtgacg gggtcagata cgacagcgac gtctagtttt tctgattcaa gatatgttag
    17461 gatttgttgt aatttagaca tatgctacct tctttctacc cctctatttt ggcaaaaaat
    17521 gagagaaaat gcaagggaga agggtaaaag aaaagacaaa aaactccgac aagataacgg
    17581 agttctttga tatcattttc ctttgtttaa gaaaagtcag ccttgctttt gacgtcactg
    17641 tactcttcag cgatttcttg gctgagaatg tcctcatagg cagggagttg gatgtcctga
    17701 ccatatttct tcttgagggc agtagtaact aggcgataag ccagattggc attacgagag
    17761 aggataggcc cgtggaagta ggaaccaaag acattcttat aatgaacccc ttcaccgacc
    17821 ttttcttcgt tgtttccatt tccatagaca acctgtccca gcggtttttg gtcatcagag
    17881 aggaaggtac gaccttggtg attttcaaat ccatagtagg tttcatcgaa atcttcattg
    17941 tgaatcttga tgtcaccgat aaaacggtta ttggtctggt tgagcgtgta gtgtcccatg
    18001 acccctagcc cttcgatacg ttttcctgaa gcttcaacat aatattgacc caatagttgg
    18061 aaaccaccgc agatagccag aactacaccg tcgttttgga tgtagttgtc aatgctctct
    18121 tttttagcag gtaggtcgtc tgcaatgata ctttgttcaa agtcttgacc accaccgaaa
    18181 aaggcgatgt cgtagtgatt ttcatcaaag tcatcatgga gagaaacgat gtcaacggtc
    18241 acatgggctc ccagtttttc agccacatac ttgagcatga ggatgtttcc attgtccccg
    18301 taggtattca tgagatttcc gtagaggtgg gcaatgttga gctgataggg gtaattgcta
    18361 tcttttgagg aaagtgaagt ataaaccatt agttcatctc ctttctaaca atctgacgac
    18421 tagccagcag ttcacgaaat tccagcatgg cagtataagt tgccagaata taggcatgct
    18481 tgcagtcttg attctcaatg gtcttgagaa cttgctccag attactcgtt tcagtgattt
    18541 tctcagctgg atagccagtc actcggaggc gacgagcgat ttcagaatga cgaacaccgc
    18601 cagcgttgat ttcaggaatg tccatgtcag tgatttgctc aaagtctgca tcccagatcc
    18661 agctagtgtc aattccatct gcatagttgg cattaaggag gacagatagg ctaaatggat
    18721 aaggtgctag tttgatcatt tcgatagctt gggttgcacc gactggattt ttaatcaaga
    18781 caagggtaca ttccttgtca ccgatatgaa aggtttcttg gcgtccaaag acagcacggc
    18841 tcttgtcaaa tccctgtttg atgagttggg aatctgcgcc gaggaaacgg gcgatggcca
    18901 cagcagctag ggcgttatag atattataga gcccgccgat ttggataccg tattcttggc
    18961 cgtctatgac aaagcgagag cgattgttgg tcaactcaac cagttttgtc aaacgatagt
    19021 cgagatcagg acgtttacat ccacaacctt cacagatata ggcacccaag tttgcatagg
    19081 tattatgctc atatttgagg atgccttggc agtcaggaca gagaatccct tcggtattgt
    19141 agtgagcaag ttgggctggt cctttttcca agtcaaaacc aaaatactct atagggtttg
    19201 gaatagttgg cttgtagaaa agtggactgt ctccgttaag gagaacagta gcagttggaa
    19261 cttttcgaat ggcatccaat atcatgttat aggtagtata gatttcaccg aaacggtcca
    19321 tctggtcacg gaagatatta gtaatgacaa aaagactagg ctggatatag tcacagatac
    19381 gagatagact ggcttcgtca atttcgagga cggcaatatt tttcccagtt tttgaagatt
    19441 tggctgttag gaaggttgtt gcaatccctg taatcatgtt ggcaccgctt gggttggtta
    19501 gaacttgacc ataaacctct tttaaaatgc cgacagtgag ggcagttgtc agggtttttc
    19561 catttgttcc agtgacaacg acaatctcgt agttcttagc taggctttgt aaaatatctt
    19621 tatcaaattg aagggcgact ttccctggga gcgtacttcc acgtccaaga cggcttaaaa
    19681 cgaagtggga agaacgccca gcaagaaggc ccaaagtagt ttttaagttc atgtttctat
    19741 tatatcacaa aagagggaaa agacagattt ggtgtttcgt agaagtaaaa acagcccaaa
    19801 gagggctgtc agttaagatg agatttcaac ttaaatcgca aacaagtcat tcaagttctc
    19861 agccaaggtt gggtgagtga agatttgttt tgtgaagtaa gtgtaaggaa tcttgttgtc
    19921 catagcaaca gtaatgatgt tgatgatttc ttgagaacct tctgagaaga tgcttgcacc
    19981 aagaatttct tttgtttcag tattaacaac agctttgaaa gctccgcgaa ggtctccatt
    20041 tacgtgacca cgaggcatgg ctgcaacagg gatttctttc actgcgtatg gaagtttcaa
    20101 atcagctgct tggctttcag tcaaaccaac ttgtgaaagt gcaggtgtga tgaacatagt
    20161 atttggcaca ttgagacggt cctcaagtgt gtagctgcca tctccagcaa ggtagctgta
    20221 aacaacacgg aagtcatcaa gtgaaatgta agtaaattga aggccaccgt tgacatctcc
    20281 aactgcaaag acaccaggaa cgtttgtttg acagtgtttg tctactttaa tagcaccacg
    20341 ttcagttagt tcaatatctg tattttcaag ttgaagtggt tctacatttg gtttgcgtcc
    20401 agttgcgtag agaagggcgt cgaaacggta agtttcgtct tcagttacga caagcacttg
    20461 gtcaccatcg tttttgattt cagtagtatg gatattttga agcaattcaa tgccatcttc
    20521 ttccatgtat tgtttagcaa gagctgcgat ggaaggttct gcacgaggta ggaatgtatc
    20581 caaggcatct aggactgtga ccttgcttcc aagtttgttg taaaggccgg caaattcaag
    20641 accgatattt ccgccaccaa ggattccaag tttttcaggt aatttgtcca agctttggat
    20701 acctgttgag tcaaagatgt ttttgcttgt agcaagtcca ggaattggca agacgtttga
    20761 aacagcacca gtgttgatga cgattgtttc agcagtcagt tctttctttt catcaccagc
    20821 ttggatttcg atgactttat ttgaaaggaa gtgagcttcc gcatcaaaga tatctacgcc
    20881 tgtaccagca acagtcgcat agtttttacc gttgaggcga ccagtgatcg tgtttttagt
    20941 agcaatcact tcttcaaaag acaagtcctt ttcagcagca actagcaagg ttttagttgg
    21001 gatacaacca atgttgatac aagttccacc gtacatagcc ttgctgcgtt caacgagggc
    21061 aacttttttg ccagctgaag ccaatttacc tgctagtgtt ttaccagctt taccaaatcc
    21121 aataacaatt aaatcgtatg ttaacattta tcttcctcct attcgaattt cattctagca
    21181 caagaaaaaa acttttgcac aattctgcta cggaaaagac agaaaaagat aaaaacgctt
    21241 tccagacaaa aaactagtaa ttttttgaca agcttgataa actaaaatcc tatctatttt
    21301 tatataagaa agaataaatg ttcgaaaata ggaggtttta tgaaatcgaa tcgttatatt
    21361 attgcctttg ctggggtcat tttacactta atgctgggtt cgacttatgc ttggagtgtt
    21421 tatcgtaacc ctattattga aaaaacggga tgggatcagg cttctgttgc cttcgccttt
    21481 agtctagcaa tcttttgttt gggcttatcg gctgcattta tggggcgttt ggtagaaaaa
    21541 tttggtccga aagtcatggg gagtctatct gcttttctat acgcaggtgg aaatatctta
    21601 acaggatttg caatagaccg tcaggaactg tggttgttgt atctagctta tggcatttta
    21661 ggtgggcttg gtttgggagc aggctatatt acccctgtgt caacgattat aaaatggttt
    21721 cctgataaac gtggtctcgc aacaggttta gcgattatgg ggtttggttt tgcttcttta
    21781 ttgactagtc ccatagcgca acacctcatc gcaggggtag ggattgtaga aactttttat
    21841 attttaggag caagttactt tattatcatg ctcctagctt cacaattcat taagcgtcca
    21901 aatgagcaag agcttgcaat tttatcttct tcagggaaag aaaaaacagc ctctttgacg
    21961 caaggaatgg ctgcaaatca ggctctaaaa agcaatcggt tttatatgct ttggattatt
    22021 ttctttatca acatagcttg tggtttaggc ttaatttcag cggcatcgcc aatggcacag
    22081 gagatggctg gcttgtctac aagtcatgca gcagtaatgg tgggtgtttt ggggattttc
    22141 aatggatttg gtcgcttgct ctgggcgagt ttgtctgact atatcggtcg ccctctaacc
    22201 tttagtatat tactgcttgt caatcttttc ttttctctct cactttggct ctttacagat
    22261 tccgttttat ttgtagttgc tatgtctatt ttgatgactt gctatggagc tggtttttct
    22321 ttgattccag cttatctcag tgatattttt ggaaccaagg aattggccgc tctgcatggt
    22381 tatattttaa cagcttgggc aatggctggt ttagcgggac ctattttatt agcagagact
    22441 tataaaatgg ctcattcgta cacacaaacc ttgttcgttt ttctcatttt atacagtatc
    22501 gccttggctt tgtcttatta tctaggtcgt tcaatcaaaa aagaaagtca aaaaccgctt
    22561 acatgatttt ctgtagaaat ggggctatct tttgctctgg tcttgtgtta tactagatag
    22621 gttgcaaaga aaacagtact tttcttttgt ggaaaagaag caacacgatt ttatatctag
    22681 ttatatgaaa atacgtcata aaaagaaaag tataaactaa gccctatagg gtggcttcgc
    22741 accaccttta gaaagaagaa taacgtgaaa tttaatgaat taaacttgtc tgctgatttg
    22801 ctagcagaaa ttgaaaaagc tggttttgta gaagctagtc ctatccaaga acaaactatt
    22861 cctttggccc ttgaaggcaa ggatgttatc ggtcaagctc agactggtac aggaaaaact
    22921 gcagcctttg gcttgcctac ccttgaaaaa atccgtatag aagaagcgac tattcaagcc
    22981 ttggtcatcg ctccaactcg tgaactagct gtccaaagtc aagaagaact cttccgcttt
    23041 ggtcgtagta agggagtcaa agtccgttca gtatatggcg gatcaagcat tgaaaaacaa
    23101 attaaggctc ttaaatctgg tgcccatatc gtggtgggaa ctccaggtcg cctcttggac
    23161 ttgattaaac gcaaggcctt gaaattacaa gacattgaaa cccttatcct tgacgaagcg
    23221 gatgaaatgc ttaacatggg cttccttgaa gacatcgaag ccattatttc ccgtgtacct
    23281 gagaaccgtc aaactttgct tttctcagca actatgccag atgccatcaa acgtatcggt
    23341 gttcagttta tgaaagcccc tgaacatgtc aagattgcgg ctaaggaatt gacaacagaa
    23401 ttggttgacc agtactatat ccgtgttaag gaacaagaaa aatttgacac catgactcgt
    23461 ctcatggatg tggcacaacc agaactcgct attgtatttg gtcgtaccaa acgccgtgtg
    23521 gatgaattga ctcgtggttt gaaaattcgt ggcttccgtg cagaaggaat tcatggcgac
    23581 ctagaccaaa acaaacgtct tcgtgtcctt cgtgacttta aaaatggcaa tcttgatgtt
    23641 ttggttgcga cagacgttgc agcgcgtggt ttggatattt caggtgtgac ccatgtctac
    23701 aactacgata ttccacaaga tcctgagagt tatgttcacc gtatcggtcg tacaggtcgt
    23761 gctggtaagt caggtcaatc tattactttt gttgctccaa acgaaatggg ttaccttcaa
    23821 atcattgaaa acttgactaa gaaacgcatg aaaggtctca aacctgcaag tgtagaagaa
    23881 tccttccaat caaaaaaaca ggtagctctc aagaaaatcg aacgtgattt tgcagatgaa
    23941 accattcgtg ccaactttga gaaatttggt aaggatgctc gcaaattggc tgctgagttt
    24001 actccagaag aattggcaat gtatatcttg agtctgacag tccaagaccc agatagcctt
    24061 ccagaagtgg agattgcacg tgaaaaacca ctaccgttta aaccatcagg taatggtttc
    24121 ggtggtaaag ctaagggagg tcgtggaggc cgtcgtgggg acgaccgtcg agagcgtgat
    24181 cgtcgtggca atggtcgccg tgatgagttc aaaaaaggaa gtcgtggcaa cgatcgtttt
    24241 gataaggaaa aacgttaccg taaggataat aaaaaaccac gcaatacttt aagcgaaaag
    24301 caaacaggct ttgttattcg taacaaaggt gataaataag aaaaagcggt tcaaaatgaa
    24361 tcgctttttt atataaggag accgaatgtg aaaaagacaa ataaaattat tatattatct
    24421 tttataatat tatgatacac aaaaataggc gacttgtcaa tagaaaagga acaatttaat
    24481 taaaaatatt ctggttttta attaaattgt aagtcaatgc aagtcaataa ggaattttca
    24541 gataattatt gaaaaagaga cgtttttatg ttataatgat agcgattaaa ttcgaggtga
    24601 aaaatggcac atttattaga aaaaactaga aaaattacat caattttgaa acgctcagag
    24661 gagcagttgc aggatgagct cccttacaac gctattacgc gtcagttagc ggatattatt
    24721 cattgcaatg cctgcattat caatagtaag ggacgtctgc ttggctattt tatgcgttat
    24781 aaaacaaata cagatcgcgt agagcaattc ttccaaacta agattttccc agatgactac
    24841 gttcaagggg ctaatatgat ttacgaaaca gaagcaaact tgcctgttga gcatgatatg
    24901 agtattttcc ctgttgagag tagagatgat tttccagatg gcttgacgac tattgcaccg
    24961 attcatgtat cggggattcg ccttggttct ttgattattt ggcgtaatga taaaaaattc
    25021 gaagatgagg acttggttct tgttgagatt gccagtaccg ttgttgggat tcagcttctt
    25081 aacttccaac gtgaagaaga tgagaaaaat attcgtcgtc gtactgctgt caccatggcg
    25141 gtcaataccc tttcttactc cgaactccgt gctgtttcag caattttagg ggaattaaat
    25201 ggaaatgaag ggaagttgac tgcgtcagtg attgcagatc gtatcggaat cactcgctct
    25261 gtgattgtca atgctcttcg taaacttgag tctgcgggga ttattgaaag tcgctcactt
    25321 ggaatgaagg gaacctatct taaggtcttg atttcagata tttttgaaga agtgaagaaa
    25381 agagattact aatgacaaag gctttaattt cgattgatta tacagaagat tttgttgctg
    25441 atagtgggaa gctgacagca ggtgctccag ctcaggcgat ttcggatgcc atcagcaagg
    25501 taactcgatt agcttttgaa cgaggagatt atatcttctt tactattgat gctcatgaag
    25561 aaaacgattg tttccatccg gaaagtaagt tatttcctcc tcataatctg attgggacga
    25621 gtggacggaa tttatatgga gatttgggga tcttttatca agagcatggt tcagacagtc
    25681 gtgtcttttg gatggataaa cgccattact cagctttttc agggactgac ctagatattc
    25741 gtttgagaga gcgtagagtg tctaccgtta tcttaacagg tgtcttgacg gatatctgtg
    25801 tcctacatac agctatagat gcctataatc taggatatga catcgagatt gttaaaccag
    25861 ctgttgcttc catctggcct gaaaatcatc aatttgccct aggtcatttc aaaaatacac
    25921 tcggagctaa gttagtagat gaaaatctaa atgaactttc tgagtaacct ggttggtgaa
    25981 aatgaaaaaa tttgaaaaaa agtgttgaca agcatcccaa aagttgatat actagtaaag
    26041 taatcgatgc ggggatggcg gaattggcag acgcgcagga ctaaggatcc tgtgaccgct
    26101 ttaggtcgtg agggttcaag tccctctctc cgcataggaa aagagttagc taaagctagc
    26161 tcttttgtat ttctctaaaa aattagaatc cagtcaaatg tagtggagaa tcagcgaaaa
    26221 tatgggaaaa ctgatccttg tttctgcttt tgtcatagaa gaataataaa gattatcttc
    26281 ttcaaattct ccgatattct ctaaagtttt gtgcaagttg cacagaactt ttttattttt
    26341 ttggtcatct tgccatagaa atataaagcg ttttcatcta taatataatt atcaaaagac
    26401 aaaaggagtt cacctcatgg tagaattgaa tcttaaaaat atttacaaaa aatatccaaa
    26461 cagcgaacac tattcagttg aagatttcaa cttgaacatc aaagataaag aatttatcgt
    26521 tttcgtagga ccttcaggat gtggtaaatc aactacactc cgtatgattg ctggtcttga
    26581 agacatcaca gaaggtactg catctatcga tggcgtggtt gtcaacgacg tagctccaaa
    26641 agaccgtgat atcgccatgg tattccaaaa ctacgctctt tacccacaca tgactgttta
    26701 tgacaacatg gctttcggtt tgaaattgcg taaatacagc aaagaagaca ttaacaaacg
    26761 tgttcaagaa gcagctgaaa tacttggatt gaaagaattc ttggaacgta aaccggctga
    26821 cctttcaggt ggtcaacgtc aacgtgttgc catggggcgt gcgattgtcc gtgatgcgaa
    26881 agtattcttg atggacgaac ctttgtcaaa cttggatgcc aaacttcgtg tatcaatgcg
    26941 tgctgaaatc gctaaaattc accgtcgtat cggagctaca actatctatg taactcacga
    27001 ccaaacagaa gcgatgacac ttgcagaccg tatcgttatt atgtcagcta ctaagaaccc
    27061 tgctggtaca ggtactatcg gacgtgtaga acaaatcggt actcctcaag aagtttacaa
    27121 aaatccagtt aacaaattcg ttgcaggatt catcggaagc ccagctatga acttcatcaa
    27181 cgtgaaattg gttggtagcg aaattgtttc tgacggtttc cgtttgaaag tgccagaagg
    27241 agcattgaaa gttcttcgtg aaaaaggcta cgaaggaaaa gaattgatct ttggtatccg
    27301 tccagaagac gtgaatgcag aacctgcttt ccttgaaaca ttcccagact gtgttgtaaa
    27361 agcgactatc tctgtatcag aactgcttgg ttcagaatct cacctttact gccaagttgg
    27421 taaagacgag tttgttgcaa aagttgatgc tcgtgactac ttgcaaacag gtgcaacagt
    27481 tgagcttgga tttgacttga acaaagcaca cttcttcgat gtagaaactg aaaaaacaat
    27541 ctactaagaa taaataaaat tcaaagcact acaagaaaag atatctcttt atcaattgta
    27601 gtggagagat atcagttaat ctagggagag aaacaaaatg cttctctcct ttttgctagg
    27661 gaagtcatat tatacatcta tattgtgatg ctctttatac tcttcgaaaa tctcttcaaa
    27721 ccacgtcaac gtcgccttgc cgtacgtatg attactgact tcgtcagttt tatctgcaac
    27781 ctcaaagctg tactttgagt agcttacggc tagtttccta gtttgctctt tgatttccat
    27841 tgagtattat ttgtgggtac catctacaag tgaagctata tgcgtaaact acgtgagcaa
    27901 ttgaattcga actagagagg taataataaa tttatgctat agttatggtg acttgtatgc
    27961 ttttgattct agtttatcaa ataatagatt agaattgtca gataatatca ttttgtgtta
    28021 taatgaagaa aaaacagagg tgttcaaatg tcagaagcag gtcataagtt tttagcaaaa
    28081 ttggggaaaa aacgcttacg tccaggtgga aagcgtgcca cagattggtt aattgcagaa
    28141 ggaggatttt caaaagaaaa gagaatacta gaggttgcgt gtaatagggg aactacagca
    28201 attgagttgg cacagcgttt tggttgcaag ataactgctg ttgatatgga tgctcaagct
    28261 ttagaagtgg ctaaaaaatc tgctggaacg gcaggtgttg ctcatttaat cagttttgaa
    28321 agagcaaatg caatgaaact tccttatcaa gatgctagtt ttgatattgt tataaatgaa
    28381 gctatgctga ctatgcaagc cgatcaagct aagaaaaaat gtgtaatgga atatctaagg
    28441 gtattaaaac ctggaggtct tctcttgaca catgatgtgc ttcttaagga agctaaagag
    28501 tctatcagac aggaattatc acaagcaatt catgtaaatg taggtccttt aactcaagat
    28561 ggttgggaac aggtgatgat agaatcaggt tattgtgatg tgaaagcatt gactggtgaa
    28621 atgacattaa tgaaattatc gggtatgatt tatgacgaag gtttgctagg aactttgaaa
    28681 atttgtgtaa atgcttgtaa aaaggagaat agaaagcagt ttttaactat gtataaaatg
    28741 tttgctaaga ataaacagaa attgggcttt attgcgatgg ctagttataa atcgtcaaaa
    28801 cgttagataa ttattgaagt taacttttcc ttttttcttt cttaaaaaat atgctataat
    28861 agagagtaaa aaactttgaa agaaagaaaa aagatgaatt taaaagatta cattgcaaca
    28921 attgaaaatt atccaaagga aggcattacc ttccgtgata ttagtccttt gatggctgat
    28981 ggaaatgctt atagctacgc tgttcgtgaa atcgttcagt atgctactga caagaaagtc
    29041 gacatgatcg tgggacctga agctcgtgga tttatcgtgg gttgtccagt tgcctttgag
    29101 ttgggaattg gttttgcgcc tgttcgtaag ccaggtaaat tgccacgcga agttatttct
    29161 gctgactatg aaaaagagta cggtgtcgat accttgacta tgcacgcgga tgccattaag
    29221 ccaggtcaac gtgttcttat tgtagatgac cttttggcga caggtggaac tgttaaggca
    29281 actatcgaga tgattgaaaa acttggtggt gttatggcag gttgtgcctt ccttgttgaa
    29341 ttggatgaat tgaacggccg tgaaaaaatc ggtgactacg actacaaagt tcttatgcat
    29401 tattaatgaa aacagtccct agggctgttt tctctacact aggatataaa aatagactat
    29461 aactagttag agaaaaacta taattgaaaa ctatatcttc ttgcagtata ataaaaggac
    29521 taagtgtttg agatttgtct tcaaacacat gcaattattc ctgagagagt acagttagga
    29581 gagggttatg ccgattcgaa ttgataaaaa attgccagct gttgagattt tacggacaga
    29641 gaatatcttt gtcatggatg atcaacgtgc tgcccaccaa gatatccgtc ctttgaagat
    29701 tttaatttta aatctcatgc cacagaaaat ggtcacagag acccagttgt tgcgccactt
    29761 ggctaataca cccctacaac tggatattga ttttctctat atggagagcc accgttctaa
    29821 aacaactcgt tcagagcaca tggagacctt ctataaaact tttcctgaag tcaaggatga
    29881 gtattttgat gggatgatca tcacgggtgc tccagttgag catttaccat ttgaggaagt
    29941 ggactattgg gaggaattta gacagatgct tgagtggtct aagactcatg tctattcgac
    30001 ccttcatatc tgttggggag ctcaggctgg gctttatctg cgctatggtg tagaaaaata
    30061 ccagatggac agtaagctat caggtattta tcctcaggac accctaaaag agggtcacct
    30121 tctatttaga ggctttgatg atagctatgt atcccctcat tcacggcaca cggagatttc
    30181 taaggaagag gtcttaaaca agactaatct cgagatttta tcagaaggac ctcaggttgg
    30241 ggtttctatt ttggccagtc gtgatttacg agaaatttat agttttggtc atttggagta
    30301 tgaccgtgat accttggcaa aagagtattt tcgagatcgt gatgcaggtt tcgacccaca
    30361 tattccagaa aattacttta aagatgatga tgttaatcag gtaccttgtc tttgttggtc
    30421 ttcatctgca gccctctttt tcagtaattg ggtaaaccat gcggtctatc aggagacgcc
    30481 ttttgactgg agaaagatag aagatgatgc atctgcatat gggtatttat aagaggaatt
    30541 atgacatatt tagacgcttt taaatcaggt accttggttt taccgagtgc cctgctcttg
    30601 cattttaagg aactctttcc ttctagcgac gattttctgg tttggcaatt tttctatttg
    30661 caaaatacga caggcttaga agaaatgtcg ccaagccaga ttgctgaaag gattggcaag
    30721 gaaatttcgg atgtcaacca gtccatttct aatctgacgg aaaggggact gctccagtat
    30781 cgtactatcg aattaaatgg cgaaattgaa ttgctctttg atgctagttt ggccttggaa
    30841 cgtttggatg acctgtttgg agcagttcat tcaagttcag accagctaac accacaaaac
    30901 cagctcaagg atttggtgga aaccttccag caggagttgg gacgattgtt gacgcctttt
    30961 gagattgagg atttgaccaa gacactaaag gaagatggaa ccagtgctga cttgattaag
    31021 gaggctcttc gtgaagctgt tttgaatgga aaaccaaact ggaagtacat tcaggcgatt
    31081 ttgagaaact ggcgccatga aggaatcaag agtgtggctc aaattgaggc caagagagca
    31141 gaaagagaag caagcaatcc tcagttgaca caggtatctg cagatttcag aaatgccatg
    31201 gatctctgga aggattaatc catgcaagta ggcttgaaat ccgagtaaga tttgcaagct
    31261 gtgtataatt gtgatagaat aaatagaaaa taaattgaaa aaagaggtat gtgaaatgtc
    31321 acgtaaacca tttatcgctg gtaactggaa aatgaacaaa aatccagaag aagctaaagc
    31381 attcgttgaa gcagttgcat caaaacttcc ttcatcagat cttgttgaag caggtatcgc
    31441 tgctccagct cttgatttga caactgttct tgctgttgca aaaggctcaa accttaaagt
    31501 tgctgctcaa aactgctact ttgaaaatgc aggtgctttc actggtgaaa ctagcccaca
    31561 agttttgaaa gaaatcggta ctgactacgt tgttatcggt cactcagaac gccgtgacta
    31621 cttccatgaa actgacgaag atatcaacaa aaaagcaaaa gcaatctttg cgaacggtat
    31681 gcttccaatc atctgttgtg gtgaatcact tgaaacttac gaagctggta aagctgctga
    31741 attcgtaggt gctcaagtat ctgctgcatt ggctggattg actgctgaac aagttgctgc
    31801 ctcagttatc gcttatgagc caatctgggc tatcggtact ggtaaatcag cttcacaaga
    31861 cgatgcacaa aaaatgtgta aagttgttcg tgacgttgta gctgctgact ttggtcaaga
    31921 agtcgcagac aaagttcgtg ttcaatacgg tggttctgtt aaacctgaaa atgttgcttc
    31981 atacatggct tgcccagacg ttgacggtgc ccttgtaggt ggtgcgtcac ttgaagctga
    32041 aagcttctta gctttgcttg actttgtaaa ataatcagta agtagcaaaa gctaggtgga
    32101 acagcattca gatgtctgtt acatttttta taggagagca aagattgaaa acaaaaattg
    32161 gattagcaag tatctgttta ctaggcttgg caactagtca tgtcgctgca aatgaaactg
    32221 aagtagcaaa aacttcgcag gatacaacga cagcttcaag tagttcagag caaaatcagt
    32281 cttctaataa aacgcaaacg agcgcagaag tacagactaa tgctgctgcc cactgggatg
    32341 gggattatta tgtaaaggat gatggttcta aagctcaaag tgaatggatt tttgacaact
    32401 actataaggc ttggttttat attaattcag atggtcgtta ctcgcagaat gaatggcatg
    32461 gaaattacta cctgaaatca ggtggatata tggcccaaaa cgagtggatc tatgacagta
    32521 attacaagag ttggttttat ctcaagtcag atggggctta tgctcatcaa gaatggcaat
    32581 tgattggaaa taagtggtac tacttcaaga agtggggtta catggctaaa agccaatggc
    32641 aaggaagtta tttcttgaat ggtcaaggag ctatgatgca aaatgaatgg ctctatgatc
    32701 cagcctattc tgcttatttt tatctaaaat ccgatggaac ttatgctaac caagagtggc
    32761 aaaaagtggg cggcaaatgg tactatttca agaagtgggg ctatatggct cggaatgagt
    32821 ggcaaggcaa ctactatttg actggaagtg gtgctatggc gactgacgaa gtgattatgg
    32881 atggtgctcg ctatatcttt gcggcctctg gtgagctcaa agaaaagaaa gatttgaatg
    32941 tcggctgggt tcacagagat ggtaaacgct atttctttaa taatagagaa gaacaagtgg
    33001 gaaccgaaca tgctaagaaa atcattgata ttagtgagca caatggtcgt atcaatgatt
    33061 ggaaaaaggt tattgatgag aacgaggtgg atggtgtcat tgttcgtcta ggttatagcg
    33121 gtaaagaaga caaggaattg gcgcataaca ttaaggagtt aaaccgtctg ggaattcctt
    33181 atggtgtcta tctctatacc tatgctgaaa atgagaccga tgctgagaat gacgctaaac
    33241 agaccattga acttataaag aaatacaata tgaacctgtc ttaccctatc tattatgatg
    33301 ttgagaactg ggaatatgta aataagagca agagagctcc aagtgataca gacacttggg
    33361 ttaaaatcat caacaagtac atggacacga tgaagcaggc gggttatcaa aatgtgtatg
    33421 tctatagcta tcgtagttta ttacagacgc gtttaaaaca cccagatatt ttaaaacatg
    33481 taaactgggt agcggcttat acgaatgctt tagaatggga aaacccttat tattcaggag
    33541 aaaaaggttg gcaatatacc tcttctgaat acatgaaagg aatccaaggg cgcgtagatg
    33601 tcagcgtttg gtattaagcg atgatttgaa agagggatgt gatagtagca ccctcttttt
    33661 ctttgtttta tgatagttca tcctcgagta aattcaagat cttgctcgga aatgaagctt
    33721 atatagtaga ttgaatatag acaaatacct tgtgattggt aaaacatttt agaaattcat
    33781 ttacctttcc taatcgactt ggtttcatct tatttcaatc tattatagta ttggggaatt
    33841 tcttcaaacc acatcagctt ggtcagttct acctgcgacc tcaaaacttg tgctttggtc
    33901 aagctgggtt tagtttccta gtttgctgat ggatttccat tgactataag catccaaccc
    33961 tctttttgtc ttctaaagaa ttcttaaatt atcagtctat tgcaactttt ctcatgtggg
    34021 tcatttggct tgcaattgtc tttccttagt agtatactga ggtagtaatc attaagaagt
    34081 ggttacataa aaataatgaa tgaggtaaaa taaaatggta gaattgaaaa aagaagcagc
    34141 aaaagatgtc gcaacattaa caaaagcagc gccagtagca ttggcaaaaa caaaggaagt
    34201 tttgaatcaa gcagttgctg atttgtatgt agctcacgtt gctttgcacc aagtgcactg
    34261 gtatatgcat ggtcgtggtt tccttgtatg gcatccaaaa atggatgagt acatggaagc
    34321 tcttgatggt catttagatg aaatcagcga acgcttgatc acacttggtg gaagcccatt
    34381 ctctactttg acagagttcc ttcaaaacag tgaaatcgaa gaagaagctg gtgaataccg
    34441 taatgttgaa gaaagcttgg aacgtgttct agctatttat cgctacctca tcactctttt
    34501 ccaaaaagcg ttggatgtga ctgacgaaga aggcgatgat gttacaaacg atatttttgt
    34561 tggtgctaag gctgaacttg agaaaacagt ttggatgctt gccgcagaac ttggacaagc
    34621 acctggtttg taagaaacaa gacaacatag ataaatctgt aggaaatttc ttacagattt
    34681 tttctatttt gtaagctatt ccaaaccagt ctttttttga gttttttgat aaaatagtac
    34741 tatcagtgaa aaggatggaa gcatgactaa gaaaatcgta gctatttggg cccaggatga
    34801 agagggtgtg attggtaagg acaatcgtct gccttggcat ttgccagcag aattgcagca
    34861 ctttaaagaa acaactctga atcatgctat cttgatgggg cgggtaacct ttgatggaat
    34921 ggggcgtcgc ttgcttccaa aacgggagac cctgattttg acgcgtaatc cggaagaaaa
    34981 gatagatggg gttgctactt ttcaggacgt ccagtctgtt cttgactggt atcaggctca
    35041 agaaaagaat ctctacatta tcggtgggaa gcaaattttt caggcttttg aagcttacct
    35101 tgatgaagtg attgtcactc acattcatgc tcgggtggaa ggagatacct atttccctga
    35161 agagcttgac ttgtctcttt ttgagacagt ttcaagcaaa ttttacgcca aagatgagaa
    35221 gaatccttat gattttacca tccaataccg caagagaaag gaagtttaat ggaacggagt
    35281 ttatttggtc tattcacggc ttttctgtgt ttcatctgct ttctggcagg agcacaagct
    35341 tttcgtaaaa agcgttacgg gctttctatc ctcctttggt taaatgcctt taccaatctg
    35401 gtcaatagta ttcatgcttt ttacatgacc ttattttaga tagaatgaca gtatagaatg
    35461 gaaggaaatc atgtctacaa atagaaaaaa tgatatgatg gtttattgct cattttgtgg
    35521 caaaaaccaa gaagaagtac aaaaaataat tgctggcaac aatgctttta tttgtaatga
    35581 atgcgtggag ttagctcagg aaatcattcg agaagaattg gttgaggaag tcttggcaga
    35641 cttgtctgag gtgccaaaac caattgaact cctccatatc ttgaaccact atgtaattgg
    35701 tcaagatcgt gccaagcgtg ccttggcagt ggcggtttat aaccactaca aacgcatcaa
    35761 tttccacgat acacgcgaag agtcagaaga tgtggatttg cagaagtcaa acattttgat
    35821 gattggccca actggttcag ggaaaacttt ccttgcccag accttggcta agagcttgaa
    35881 tgtacctttt gctattgcgg atgcgacagc tctgacggag gctggttatg tgggtgagga
    35941 tgtggaaaat atactcctca aactcttgca ggttgctgac tttaacatcg aacgtgcaga
    36001 gcgtggcatt atctatgtgg atgaaattga caagattgcc aagaagagtg agaatgtgtc
    36061 tatcacacgt gatgtttctg gtgaaggggt gcaacaagcc cttctcaaga ttatcgaggg
    36121 aactgttgct agcgtaccgc ctcaaggtgg acgcaaacat ccacaacaag agatgattca
    36181 agtggataca aaaaatatcc tcttcatcgt gggtggtgct tttgatggta ttgaagaaat
    36241 tgtcaaacaa cgtctgggtg aaaaagtcat cggatttggt caaaacaata aggcgattga
    36301 cgaaaacagc tcatacatgc aagaaatcat cgctgaagac attcaaaaat ttggtattat
    36361 ccctgagttg attggacgct tgcctgtttt tgcggctctt gagcaattga ccgttgatga
    36421 cttggttcgc atcttgaaag agccaagaaa tgccttggtg aaacaatacc aaaccttgct
    36481 ttcttatgat gatgttgagt tggaatttga cgacgaagcc cttcaagaga ttgctaataa
    36541 agcaatcgaa cggaagacag gggcgcgtgg acttcgctcc atcatcgaag aaaccatgct
    36601 agatgttatg tttgaggtgc cgagtcagga aaatgtgaaa ttggttcgca tcactaaaga
    36661 aactgtcgat ggaacggata aaccgatcct agaaacagcc tagaggtgac tatggaactt
    36721 aatacacaca atgctgaaat cttgctcagt gcagctaata agtcccacta tccgcaggat
    36781 gaactgccag agattgccct agcagggcgt tcaaatgttg gtaaatccag ctttatcaac
    36841 actatgttga accgtaagaa tctcgcccgt acatcaggaa aacctggtaa aacccagctc
    36901 ctgaactttt ttaacattga tgacaagatg cgctttgtgg atgtgcctgg ttatggctat
    36961 gctcgtgttt ctaaaaagga acgtgaaaag tgggggtgca tgattgagga gtacttaacg
    37021 actcgggaaa atctccgtgc ggttgtcagt ctagttgacc ttcgtcatgc cccgtcagca
    37081 gatgatgtgc agatgtacga atttctcaag tattatgaga ttccagtcat cattgtggcg
    37141 accaaggcgg acaagattcc tcgtggtaaa tggaacaagc atgaatcagc aatcaaaaag
    37201 aaattaaact ttgacccgag tgacgatttc atcctctttt catctgtcag taaggcaggg
    37261 atggatgagg cttgggatgc aatcttagaa aaattgtgag gaaaagaaaa tggcaaaaac
    37321 aattcataca gataaggccc caaaggctat cggaccctat gttcaaggaa aaatcgttgg
    37381 caaccttttg tttgctagcg gtcaagttcc cctatcccct gaaactgggg aaattgtagg
    37441 agagaatatc caagaacaga cagagcaagt cttgaaaaac atcggtgcta ttttggcaga
    37501 agcaggaaca gactttgacc atgttgtcaa aacaacttgc ttcttgagcg atatgaacga
    37561 ctttgttcct tttaatgagg tttaccaaac ggccttcaaa gaggaattcc cagctcgttc
    37621 agctgtggag gtagctcgtc ttcctcgtga tgtaaaagtc gaaattgaag tcatcgcaga
    37681 gattggataa gctagttgaa gtttggtgtt gccaaacttc ttttgatata aggagaaaaa
    37741 gatgacaaag aaacaacttc acttggtgat tgtgacaggg atgagtggcg cagggaaaac
    37801 tgtagccatt cagtccttcg aggatctagg ttatttcacc attgataata tgccgccagc
    37861 tctcttgcct aagtttttgc agctggttga aattaaggaa gacaatccta agttggcctt
    37921 ggtagtggat atgcgtagcc gttctttctt ttcagagatt caagctgttt tggatgagtt
    37981 ggaaaatcaa gatggtttgg atttcaaaat cctctttttg gatgcggctg ataaggaatt
    38041 ggtcgctcgt tacaaggaaa ccagacggag tcacccacta gcagcagacg gtcgtatttt
    38101 agatggaatc aagttggaac gtgaactctt ggcacctttg aaaaatatga gccaaaatgt
    38161 ggtggatacg actgaactca ctccacgtga gctgcgtaaa acccttgcag agcagttttc
    38221 agaccaagaa caagcccagt ctttccgtat cgaagtcatg tctttcggat ttaaatatgg
    38281 aatcccgatt gatgcggact tggtctttga tgtccgtttc ttgccaaatc cctattatct
    38341 accagaactg agaaaccaaa cgggtgtgga tgaacctgtt tatgattatg tcatgaacca
    38401 tcctgagtca gaagactttt atcaacattt attggccttg attgagccga ttctgccaag
    38461 ttaccaaaag gaaggtaagt ccgttttgac cattgctatg ggatgtacgg gtggacaaca
    38521 ccgtagtgtg gcatttgcta aacgcttggt gcaggactta tccaagaatt ggtctgttaa
    38581 tgaagggcat cgcgacaaag accgcagaaa ggaaacggta aaccgttcat gagaaaacca
    38641 aagataacgg tgattggtgg agggactgga attcccgtca ttctaaaaag tctgcgggaa
    38701 aaagatgtgg aaatcgcagc tatcgtgacg gtggcagatg atggtggttc ttcaggtgaa
    38761 ctccgaaaaa atatgcaaca gttgacaccg ccaggtgatc ttcgtaatgt ccttgtggcc
    38821 atgtcggata tgcctaagtt ttatgagaag gtctttcagt atcggttctc tgaggatgcc
    38881 ggagcctttg ctggccatcc attgggaaat ctcatcattg ctggcttgtc agaaatgcag
    38941 ggttcaacct ataatgccat gcagttattg agcaaatttt tccatacaac agggaaaatt
    39001 tatccttcca gtgaccatcc tttgaccctt catgcagtct ttcaggatgg gacagaagtg
    39061 gctggagaga gtcatattgt agaccatcga ggcataattg acaatgtcta tgtgaccaat
    39121 gccctaaacg atgatacgcc tctggccagc cgtcgagtag tgcagaccat ccttgaaagt
    39181 gacatgattg tcctagggcc aggttccctc tttacctcta ttttgcccaa tatcgtgatt
    39241 aaggaaattg ggcgggctct tttggaaact aaggcagaaa ttgcctatgt ctgcaatatc
    39301 atgacccaac gtggggagac ggaacacttt acagatagcg accacgtgga agtcttgcat
    39361 cgtcaccttg gtcgcccttt tatcgacact gtcttggtga atattgaaaa agtgcctcag
    39421 gaatacatga attccaaccg ttttgatgaa tacttagtgc aagtggaaca cgattttgta
    39481 ggtctttgta agcaagtttc gcgcgtgatt tcatctaact tccttcgtct ggaaaatggc
    39541 ggtgccttcc acgatggaga tttgattgtg gacgagttga tgcgcattat acaggtgaaa
    39601 aaatgagttt cacagtagca gtaaaagaag aaatcctggg ccaacaccat ctgagccggc
    39661 atgaattatc tgccattatc aaaatgtctg gtagcatcgg tctctcgact tcgggcttga
    39721 ctttgtcagt tgtgacagaa aatgccaaac tggcccgtca cctctatgag tcctttctcc
    39781 atttctatga aatcaaatct gaaattcgtc accaccaacg gagcaatctt cgcaagaatc
    39841 gcgtctatac cgtttttaca gatgaaaagg tgcaggattt gttaagtgat ttgcacttgg
    39901 cagactcttt ctttggcctg gaaacaggta ttgatgaggc gattttatcg gacgaggaag
    39961 caggtcgtgc ctatctctgt ggcgctttct tggcaaatgg gagcattcgt gaccctgagt
    40021 caggcaagta ccagttggaa atcagttctg tttatctgga ccacgcgcaa ggaattgcct
    40081 cccttctcca acagttttta ctggatgcca aggtgcttga gcgcaagaag ggggctgtga
    40141 cctatctcca gcgagcagaa gacattatgg acttcttgat agtaatagga gccatgcagg
    40201 cacgtgatga ttttgagcgg gttaagattt tgcgagaaac ccgtaacgac ctcaatcggg
    40261 ctaataatgc cgagacagct aatatcgctc ggacagtttc tgccagcatg aagaccatta
    40321 acaatatcag taaaatcaaa gatatcatgg gcttagaaaa tttgccagta gatttgcagg
    40381 aagtggcgca actgaggatt cagcacccag actactctat ccagcagtta gcagatagtc
    40441 tcagcacccc tctgaccaaa agtggtgtca atcacagact cagaaaaatc aataagatag
    40501 cggatgaatt ataaaaccac gaatcctatg tgactcgtgg ttctttttta taaactggta
    40561 gagtgttttg gttgtacttt ttgttcaggg tcaatgtagt tgatagcgtt gttgacagca
    40621 gttggagctt ctccgaggcc tgtcgcaatc agatcaattt ttccgtcata gtagcagcag
    40681 tcaccgatag catagatacc tgcttggctg gattcctgtt tgctgttgac gataatcttg
    40741 tgacggttga ggtcgagccc ccagttttta aggttaccga cagaagattt gaaaccatag
    40801 ttgacaaaga ggtggtctag gtcaatggtt tcagtttcat cagatttgac ttttgtgatt
    40861 tcaagtttat caagtgtttt tccatttcca aggagttggc taggggcgaa tggtgtcttg
    40921 atggttacag atgattcttg caaggtttga acactgtgtt ccaaggcacg gaaattatct
    40981 ctgcggtgaa caagggtagt tggtgcgatt ttttcaaaag ccaaagccca atccacagcc
    41041 gagtctcccc caccaagaat cgtcactttc ttaccagcgt attgctgaat gttagaaacg
    41101 tggtagtgga tattttcata gtcctcaacc ccttcaagtt ccagcggacg tggtttgaag
    41161 gcaccgccac ccatagcgat gataactgtt ttagtcaggt gacttccttt ggaagttgtg
    41221 atggcaaatt cttcttcttg tttgtcaatc tcaagaaccg tttcattgag atgaataggg
    41281 gtatcaaatc catttagctg ttcaatcaag cggttagtca actcttctcc agtcaggttt
    41341 gggaagcctg gtacgtctag gatttccttt tcagggtaga gaatagcagg ttgtccacct
    41401 agctggggaa gagagtcgat gatttgaacc ttggcttggc gtaggtgggc ataaaaggct
    41461 gcaaaaagcc cgacaggacc accacccaca atggtaatat catagagttg agacatagtt
    41521 tctcctttgt tttttctagt cagtttattt tatcatattt ttgttcaatg taaaatggac
    41581 taggataggg aaaaaaatgc cagaaaatgg tataatagga cgaaagtgtt ttgataggga
    41641 ggatatcagg gatgaatttt caacaattat ccaatctgca atattggacc agtttgtttg
    41701 caagtccatg gacgatagct atcaatctga ttgatatttt gattgttgct tatattttat
    41761 accattttac aaaagctatt gcaggaacca agattatgat tttggtacgt ggagttttgg
    41821 tgtttatttt agctcaaatc cttgcaaata tgattggttt gactacgatt tcttggttaa
    41881 tcaatcaaat tattacttat ggggttattg cggcggttgt tatcttctct ccagagattc
    41941 ggactggttt ggaacgtttg ggaagagcga cagatttctt ttccaatgcc cctattagtg
    42001 ctgaggaaca gatgattcgt gcctttgtta agtctgttga atacatgagt cctcgtaaaa
    42061 tcggggcctt ggttgctatt cagcgtgtac gtaccttgca ggagtatatg tcgacaggaa
    42121 ttcccttgga tgctaagatt tctgcagaac ttctcattaa catttttatt cccaacactc
    42181 ccctacatga cggtgcggtg attatcaaag aagaacgtat cgctgtgacg tctgcctatc
    42241 tgcccttgac aaaaaacaca gggatttcca aggaatttgg gaccagacac cgggcggcta
    42301 tcggtttatc agaagtctca gatgccttga cttttgtcgt atcagaggaa acgggaggaa
    42361 tttcgataac ctataatgga aggtttaagc acaacctaac acttgatgaa tttgaaacag
    42421 aattacgtga aatcttactt ccaaaagagg aagtgggtct tagttttaaa gaacgattgc
    42481 taggaggatg gaaacatgaa aaaaaatagt ttatatatca tatcctcact cttttttgct
    42541 tgtgtcttat ttgtctatgc tacggcgacg aattttcaaa acagtaccag tgctaggcag
    42601 gtaaaaacgg aaacctatac taatacagta accaatgtcc ctattgacat acgctataat
    42661 agtgataagt attttattag cggttttgct tcagaagtat cagtggtctt gactggtgca
    42721 aatcgcctat cgctagctag tgaaatgcaa gaaagtacac gtaaattcaa ggttactgct
    42781 gacctaacag atgccggtgt tggaacgatt gaagttcctt tgagcattga agatttaccc
    42841 aatgggctga ccgctgtggc gactccgcaa aaaattacag tcaagattgg taagaaggct
    42901 cagaaggata aggtaaagat tgtaccagag attgacccta gtcaaattga tagtcgggta
    42961 caaattgaaa atgtcatggt gtcagataaa gaagtgtcga ttacgagtga ccaagagaca
    43021 ttggatagaa ttgataagat tatcgctgtc ttgccaacta gcgaacgcat aacaggtaat
    43081 tacagtggtt cagtaccttt gcaggcaatc gatcgcaatg gtgttgtctt accggcagtt
    43141 atcactccgt ttgatacaat aatgaaggtg actacaaaac cagtagcacc aagttcaagc
    43201 acatcaaatt caagtacaag cagttcatcg gagacatctt cgtcaacgaa agcaactagt
    43261 tcaaaaacga attaaaaata gaaaaggatt ttataacaat gggtaaatat tttgggactg
    43321 atggagtccg tggagaagct aacctagagc taacaccaga attagccttt aaactaggac
    43381 gttttggagg ctatgttctt agtcaacatg aaacggaagc gccgaaagtc tttgtaggac
    43441 gtgacacacg tatttcaggg gaaatgctgg aatcggcctt ggtggcaggt ctcctttcag
    43501 tagggattca cgtatacaaa cttggtgtcc ttgcaacacc agcagtagct tacttggttg
    43561 aaactgaagg agcaagtgcc ggtgtcatga tttctgctag ccacaaccca gcccttgata
    43621 acggaatcaa gttctttggc ggtgatggct tcaaactaga tgatgaaaaa gaagcagaaa
    43681 ttgaagcctt gctagatgct gaggaagaca ctcttcctcg tccaagtgca gaaggcttag
    43741 gaattttggt agattatcca gaaggcttgc gtaagtatga aggatacctt gtttcaactg
    43801 gaactcctct tgatggaatg aaggttgcct tggatacagc taatggagca gcttctacca
    43861 gtgcccgtca aatctttgca gaccttggtg cccaattgac ggttatcggg gaaacaccag
    43921 acggtcttaa catcaacctt aatgttggtt caacacatcc agaagccctt caagaagtgg
    43981 tcaaagaaag tgggtcagct attggtttgg cctttgatgg agacagtgac cgcttgattg
    44041 ctgttgatga gaatggtgac atcgtcgatg gtgacaagat tatgtacatc atcggaaaat
    44101 acctttctga aaaaggacaa ttggctcaaa atacaattgt gacaactgtt atgtctaacc
    44161 ttggtttcca caaggccttg aatcgcgaag gtattaacaa ggcagttact gcagttggtg
    44221 accgctacgt tgttgaagaa atgagaaaat caggttacaa ccttggtggt gaacagtctg
    44281 gtcacgttat cttgatggat tacaatacca caggtgatgg tcaattatca gcagttcaat
    44341 tgactaaaat catgaaggaa actggtaaga gcttatcaga gttggcggca gaagtaacga
    44401 tttatccaca aaaattagtt aatatccgag tggaaaacgt catgaaggaa aaggccatgg
    44461 aagtgccagc tatcaaggcc atcatcgaga agatggaaga agaaatggcg gggaacggcc
    44521 gtatccttgt tcgtccaagt ggaacagaac ccctcttgcg tgttatggca gaagcgccta
    44581 caacagaaga agtagactac tatgttgata ccatcacaga tgtagttcgt gctgaaattg
    44641 ggattgacta aatcctagaa aactagatga aaataaaaaa ttatggtaca atagcttata
    44701 atattgtagc cgatggagaa agacatgaat cttaaacgtg aacaagaatt tgttagtcag
    44761 tatcattttg atgcgcgtaa ttttgaatgg gaaaatgaaa atggagctcc tgaaaccaag
    44821 gtggatgtga atttccaatt gctccaacat gaccaagaaa atcaagtgac ttccttgatt
    44881 gtcatcttga gctttatgat tgtatttgat aagtttgtca ttagtggaac aatctcacaa
    44941 gtaaaccata tcgatggccg tattgtcaat gaaccaaatg aattgaacca agaagaggtg
    45001 gaaaccttgg ctcgtccatg tttgaacatg ctcaatcgtt tgacttacga agtgactgaa
    45061 attgcattag acttaccagg aattaatttg gagttctaat atgaaattag ctgttttcac
    45121 agattcctct gcttatctca gtgcagagat cttgcaaaga gaagacttgt ttgtcttgga
    45181 tattcctgtc aatattgatg gtgaggaata tgtcgaaggc atcaatctgt ctgctgagga
    45241 attttaccaa aaaatggctc aggcttctga attgcctaag accagtcaac caagtattgc
    45301 caagttagat gagatcttaa cttcgctcaa agaacaaggc tatacacatg ccttggggct
    45361 tttcctatct tctggaattt caggttttta ccaaaatatc cagtatatgg tcgatgacta
    45421 tgagggctta accattgctt tcccagacac tttgattaca agtgctcccc taggcatcat
    45481 ggttgaaagt gtctttaatt ggcgtgatca gggcgatgat tttgccagca ttcaggataa
    45541 gctagccatt caaatcagcc gtacgtcagc ctttatcatg gtagatgact tggatcattt
    45601 ggtaaaaggt ggacgccttt caaatggggc tgccattttg ggcaatttgc ttagcattaa
    45661 gccaatcctt tattttaacg atcaaggtgt gattgaagtt tacgaaaaag ttcgtactga
    45721 aaagaaggcc accaagcgct taattgaaat tatcaaggaa acaacggctt caggtcaata
    45781 ccgggtcatt gtcattcatg ggaatgctcc tgaaaaggct gaagaattgc gtcagcactt
    45841 gcttgatttt ggcttgggtt cggatgtttc acttgctaca tttggtagtg tcattggaac
    45901 gcacctagga gcaggaagta ttgctctggg ttatattcca gtgatttagt tggcgcttct
    45961 aggctagtta agatgccttg tatcttagaa attgaacacg gactacctgc ctcttgaaaa
    46021 aagatgcctg tcttgccttt cgtggaaagt cagtgccatt ccctattttt cagaggcagc
    46081 taaggtcctt tgtatcttga taattgaaca cgcctggaac cctgtgtgaa aaagatagtt
    46141 cttccaagaa gtagacactt cttgattgga attcctattt tcactttgtg ttcttacgga
    46201 ctttgtatct tagacaggag tagagatgag tattcgagta attattgccg gttttaaggg
    46261 aaagatgggc caggctgctt gtcagatggt attgactgat ccagacttgg acttggtggc
    46321 agttttggat ccttttgagt ctgagtcaga atggcagggt attcctgttt tcaaggataa
    46381 ggctgattta gctggttttg aagcggatgt ctgggtagat tttactactc cagctgttgc
    46441 ctacgaaaat acacgttttg ctcttgaaaa tggctttgct ccagtagttg gaacgactgg
    46501 tttcacgagt gaagaaattg cagagctaaa agaattttct cgtgcccaag acttgggtgg
    46561 cctgattgcc cctaactttg ccttgggtgc tgtcttactc atgcaatttg cgacgcaggc
    46621 tgccaaatat ttcccaaatg tggagattat tgagctccat catgacaaga aaaaggatgc
    46681 tccgagtgga acagccatta aaacagctga gttgatggca gaggttcgag agtccattca
    46741 gcaaggtgca gcagatgagg aagagctgat tgctggtgct cgtggtgctg actttgatgg
    46801 tatgcgcatc cactcagttc gtttgccagg cttggtagct catcaggaag tcatctttgg
    46861 caatcaggga gaagggttga ccctccgtca tgactcctat gatcgcatct ccttcatgac
    46921 aggagtcaat ttgggaatta aagaagttgt caagcgtcat gagcttgtct atggattaga
    46981 acacttatta tgagattaat gcaaatgcct tctgaatttc agaaggcttt accagtatta
    47041 gaaaaaatta aagaagcagg ctttgaggct tattttgttg ggggctctgt tcgagatgcc
    47101 cttctccata gccctatcca cgatgtggat attgcgacgt cttcttatcc agaagagacc
    47161 aagcagattt ttccgcgaac agccgatatc ggaatcgagc atggaaccgt cttggtctta
    47221 gatggggatg aggagtatga ggtaacaacc tttcggacag aggatgtcta tgtggactat
    47281 cgcagaccca gtgcggtttc ctttgtgcgc tcgctagaag aagacctcaa acgccgtgat
    47341 ttcacagtca acgcctttgc cttggatgag acaggagaaa tcgttgactt gttccatggt
    47401 ttagaagatt tggaaaagca agtcttgcga gcagttggag tggctagtga gcgtttcaac
    47461 gaagatgctt tacggattat gcgtggtttc cgttttcagg ctagtcttgg ttttgcactt
    47521 gagccagaaa catttaaagc tatgaagacc ttgacgccgc ttttggagaa aatttctgta
    47581 gagcgtacct tcgtcgagtt tgataaactc ttgctggctc cattttggag aaggggcttg
    47641 gcttccatga ttgagagtca agcttatgac tatctccctg atatggcatc tagccaggac
    47701 aagctcaaca gactgtttga tttggagact gattttactt ttgaatcctc tgaacaagcc
    47761 tgggcggctt tactatgggc tttggagatt gaaaatgcgc agtcattttt gaaatcttgg
    47821 aagacctcac gccagtttgc caagcaagtt caggatttgc tgattatttt ggctctgcgt
    47881 gaaaatggag aattgagtaa gcgtgattgt tatcgctttg acttggattt acttttacag
    47941 gctgaaaatc ttcgtcaagc tcagggaaaa gaagtcaacc cacaagtcat taaagaaacc
    48001 taccagagct tgactattca tgataagaaa gaaatccaaa tcaacggcgg tattttgatc
    48061 aaggaatatg gctatcagcc aggcccagac ttgggagaga ttttaacaga gattgagttt
    48121 gccattgtcg atggagaatt ggagaataat cgtgaagcca tccatgctta cctgagggag
    48181 aaaaaatgag tgattttatc gttgaaaaac taagtaaatc cgttggtgac aagaccgttt
    48241 ttagggatat ttcctttatt atccatgact tagacagaat tggtttaatc ggtgtcaatg
    48301 ggactggcaa gaccaccctt ttggacgtcc tttctggtgt ttctggattt gatggggatg
    48361 tcagtccttt ttcagctaaa aatgattacc agattggtta cttgactcag gatcctgatt
    48421 ttgatgatag aaagacagtt ttggatacgg ttctatctag tgaactcaag gaaatccagc
    48481 tcattcgtga gtatgaattg attatgctcg actatagtga ggacaagcag gcgcgtttgg
    48541 aacgtgtcat ggcagagatg gactctctcc aagcttggga aattgagagt caggtcaaga
    48601 ccgttcttag caaattgggc attcaagact tatctactcc tgttggggaa ttgtcaggtg
    48661 gtctgagaag acgggtacag ttggcacaag tcttacttgg caaccacgac cttttgcttt
    48721 tggatgagcc gaccaaccat ctggatattg cgattattga gtggctgacc ctctttttga
    48781 aaaattctaa gaagaccgtc ctttttatca ctcacgatcg ttatttctta gacgctttgt
    48841 caacacggat tttcgagttg gagcgagcag gcttgacaga atatcagggc aattaccagg
    48901 actatgttcg ccttaaggca gaacaggacg agcgcgatgc agctctactt cataaaaaag
    48961 aacaactcta caaacaagaa ctggcctgga tgcgcagaca accgcaggca cgtgcgacca
    49021 agcaacaagc tcgtatcaat cgtttccatg atctgaaaaa ggaagtttca ggcagtagtg
    49081 ctgagacaga cttgactatg aactttgaaa ccagtcggat tgggaagaaa gtcatcgagt
    49141 ttcaggatgt ttcctttgcc tatgaaaata agcccatttt gcaaaatttt aatctcttag
    49201 ttcaggctca agaccgtatt ggaattgttg gggacaatgg tgttggaaaa tcaaccctac
    49261 ttaacctgat tgcaggaagt cttgagccga cagcaggaca agttgtgatt ggggaaactg
    49321 ttcgcatcgc ctatttctct caacaaattg agggtttgga tgaaagcaag cgtgtgatca
    49381 attacctgca ggaagtggca gaggaggtca agaccagtgg tggttctacg acttccatcg
    49441 ctgagttgct ggagcaattc ctcttcccac gttcgacgca tgggactttg attgagaaat
    49501 tgtcaggggg tgagaaaaaa cgtctttatc tcctcaaact gcttttggaa aaaccaaatg
    49561 ttcttctttt agacgagcca accaatgacc tagatattgc aactttgaca gtcttagaga
    49621 atttcttgca aggttttgca ggtcccgttt taacagtcag tcacgaccgc tatttcttgg
    49681 ataaggtagc gaccaagatt ctcgcttttg aggatggcaa gattcgtcct ttctttggtc
    49741 attacaccga ctatcttgat gaaaaagctt ttgaaacaga tatggccaat caagtgcaaa
    49801 aggccgaaaa ggaaaaagtg gtcaaggttc gagaagacaa gaaacgcatg acctaccaag
    49861 aaaagcagga gtgggcaagt attgaaggtg atattgaaac cttggaaaaa cgtatcgctg
    49921 ctattgaaga ggaaatgcag gctaacggct ctgactttgg taagctggct actctccaaa
    49981 aagaattgga tgagaaaaat gaagcactcc ttgaaaaata cgaacgctat gagtatctca
    50041 gtgaatttga tagttaagac tattcgaaaa tcaaagtgca aactatgtcg gcttcacatt
    50101 gccgactcaa gtacagcctg tgactggctt cctaatttgc actttgattt ttattgagta
    50161 taaaagaata gaaaaatccc gtctcatgaa aggatttctc tattcttttt tggtttcttg
    50221 atgaaagata ttttgccagg tttcgtggcg acgccagata ctggtatgga tgataccatc
    50281 taactcatag cagatgagtt tggttttttg actgatggaa gtaatttgaa tgtccttgat
    50341 agcagaattt aactcttttt tggtcttata agcctcttta tccatctgct ctccatcttg
    50401 gcgaatatag acaaaatcat cagtcaagag ttcttctagt tggtttcctt ggtcaatcaa
    50461 ttgttcacgc ataagcaatt ttttatagac attgtctaaa atttcatcct cagggatagg
    50521 tgctggttcg atatggacat cggtatcaaa gacgccaaaa cgctcctcca gcatagactc
    50581 gacctgatcc gcgatttcat ggctttcaaa aacagacaag tcaggattca tctctagtgt
    50641 aatatccagg tagatgttgc taccgtaggt gcgacctctt tgcgatttga ccttgctgat
    50701 tttgggaatt tccatgatag ccttttggta gtcctcgagc aggcggtcgt caaagccatc
    50761 tgaaagacta aaggaagact cgatgaagat atcataggca gtcttcaaga taaagaaagt
    50821 gatgatgata gcaaccagtt tatccacaat cggataatta aaactactag ctaggatggc
    50881 aatggcggtt ccaagtgagg taacagcgtc agaaagattg tccttagcag ctgccttcag
    50941 cgccttggag ttggatttct tactgaggcg agtattgtag agatagacca caaacataat
    51001 cgctgcagaa atgattccta gagttgcacc aagaggatca atgaccgttt cttcccgact
    51061 gagaatcttt tgaatggtat ctcttagaac atcgaaaccg acatagaaca tgatgataga
    51121 agtgatcaag cttgccaaat cttcaatctt ccaatgacca aaacggtggt cacggtctgc
    51181 aggctggcgc gccatccgaa tcccgattaa gagggctaca tttccaatga tgtccgatac
    51241 gttattaaaa ccatcggcca ccaaactgga tgaatgaagg agatgaccag ctgctaattt
    51301 ggctgcagac aagatcaaat aggtcgaaat actgataatg gctccacgct cagctaactt
    51361 gagatttgag atagattgct tcattcaact tcctttctag tcacatagca ttctaccatt
    51421 atactggttt tcaaggaaaa attcaaataa ggcagttttt actctttgaa aactccccaa
    51481 aatttggtat aatggtacta ctcaagggag tagctggcag aaacctgtga tagtgtcgtc
    51541 attccgaatt ttatgctgaa aagtatgctt tccggcccta tcttaaacag cgagacttgt
    51601 tatgattaac aggtctcttt ttgtttgtcg tgaaaagata tgacagaaag agagtctgtt
    51661 ttgcacacaa tgtcaaggag gagacacatg tcaaaagaac aaaaacgcca agcgttttat
    51721 actcagagcc ctgaagaggt cttgcaggct gtggatgcga ccgagcaagg tttgtcatca
    51781 agtgaggcgg aaaagcgcct tgccgaattt gggcacaatg aactcgaaga aggcgagaaa
    51841 cgatcaatct tggtcaaatt catcgagcaa tttaaggatt tgatgattat catcctagtt
    51901 gcggcagcaa tcttgtcagt cgtgacttct ggtggggaag atatcgcaga tgccattatc
    51961 atcctagctg tggttatcat caacgctgcc tttggtgttt accaagaagg aaaagctgaa
    52021 gaagctattg aagccctcaa atccatgtct agtccagttg cccgcgttct tcgtgatgga
    52081 cacatggcag agattgactc taaagaattg gtaccaggcg atatcgttgc ccttgaagca
    52141 ggtgacgtgg taccagcgga cctacgtttg atagaagcca actctcttaa aattgaagaa
    52201 gcagccttga caggtgaatc tgtaccagtc gaaaaagact tgtcagtcga gcttgcgaca
    52261 gatgctggta ttggtgaccg tgtcaacatg gccttccaaa actcaaacgt aacctatggt
    52321 cgtgggatgg gtgttgttgt caatacaggt atgtacactg aagttggtca tattgctggt
    52381 atgcttcaag atgcggatga gactgataca ccactcaaac aaaatttgaa caacctttct
    52441 aaggtattga cctatgctat cttggtcatt gcccttgtta cttttgtagt gggtgtcttc
    52501 attcaaggga aaaatccact tggtgagttg ttgacttctg ttgcccttgc cgttgcagcc
    52561 attccagaag gacttcctgc tatcgttacc atcgttcttt ctcttggtac tcaagttttg
    52621 gccaaacgac attccatcgt tcgtaagttg ccagcagttg aaacacttgg ttcaactgaa
    52681 atcatcgctt ctgataagac tggtacgctg actatgaaca agatgacagt cgaaaaagtc
    52741 ttctacgatg cggttctaca tgactcagct gatgatattg aactaggtct tgaaatgcca
    52801 ctacttcgtt cagttgtctt ggccaatgat acgaaaatcg atgtggaagg taacttgatt
    52861 ggtgacccaa ccgaaacagc ctttatccaa tatgccttgg acaagggcta tgatgtcaaa
    52921 ggtttcttag agaaatatcc tcgtgtagct gaattgccat ttgactctga ccgtaagctc
    52981 atgtcaacag ttcacccatt gccagatggt cgtttccttg tagcagtcaa gggtgcgcca
    53041 gaccaactct taaaacgttg tcttcttcgt gataaggctg gggatattgc tccgattgat
    53101 gagaaggtta caaatctcat tcatacaaac aattctgaaa tggctcatca agccttgcgt
    53161 gtccttgcag gtgcttataa gattatcgat agtattccag aaaatctcac ttctgaagag
    53221 cttgaaaatg atttaatttt tactggtttg attgggatga ttgaccctga acgtcctgaa
    53281 gccgctgagg ctgttcgtgt ggctaaggaa gcgggaatcc gtccaattat gattacaggt
    53341 gaccaccaag acacagcgga agccattgcc aaacgtttgg gaatcattga cgcaaacgat
    53401 acagaaggtc acgttttaac tggtgctgaa ctcaatgaac tgtcagatga agaatttgaa
    53461 aaagtcgttg gtcaatactc tgtttatgcc cgtgtgtctc cagaacacaa ggttcgtatc
    53521 gtcaaggctt ggcaaaaaca aggtaaagtc gttgccatga caggtgatgg tgtcaatgac
    53581 gcgccagctc tgaaaacagc cgatatcggt atcggtatgg gaatcactgg tacagaagtt
    53641 tctaaggggg cttctgacat gattcttgca gatgataact ttgcgactat tatcgtcgca
    53701 gtggaagaag gacgtaaggt cttctcaaac attcaaaaga ctattcagta cctactttct
    53761 gctaatactg ctgaagtatt aaccatcttc ctatcaacct tgtttggttg ggatgtcttg
    53821 cagccggttc atcttttgtg gatcaacttg gtaacggata ccttcccagc tattgctctt
    53881 ggtgttgaac ctgcggagcc tggtgtcatg aatcataaac cacgtggacg caaggcaagc
    53941 ttcttctcag gtggtgtttt gagttctatc atttatcaag gtgtactcca agcagctctt
    54001 gttatgagtg tttatggcct tgcgattgct tacccagttc atgtgggtga caatcatgct
    54061 attcatgcag atgccctaac gatggccttt gcaacccttg gtttgattca gctcttccat
    54121 gcctacaatg tcaagtctgt ctaccaatcc atcttgacag ttggcccatt caagtctaag
    54181 acctttaact ggtccatctt ggtatccttt atccttctca tggcaacaat cgttgtagaa
    54241 ccgcttgaag gaatcttcca cgtaaccaaa ctagacttgt cacaatgggg aattgttatg
    54301 gctggaagct tttcaatgat tatcatcgtc gaaatcgtta agtttattca acgcaaactc
    54361 ggttttgaca aaaatgcgat ttaagggagg tttcggatgt cagcatatca attaccgacc
    54421 gtatggcagg atgaagctag taatcaagga gcttttacgg ggctaaacag accaacagca
    54481 ggtgcccgtt tcgaacaaaa cttgccaaaa ggagaacaag cttttcagct ttattcactg
    54541 agaacaccaa atggtgtgaa ggttactatc ttattggaag aattactaga agctggtttt
    54601 aaggaagcgg cttacgactt gtataagatt gctatcatgg atggggatca attcggatca
    54661 gactttgtga agctcaatcc aaattccaag attccagcct tattggacca gtcaggtact
    54721 gaagatgtaa gagtctttga gtctgctcat attcttcttt accttgctga gaaatttgga
    54781 gcctttttac caagtaatcc tgtggaaaag gtagaagttt tgaattggct attctggcaa
    54841 gcaggtgcag caccttttct aggtggggga tttggacatt tcttcaatta tgctcctgaa
    54901 aaattggaat atcctattaa ccgttttacg atggaagtga aacgccagtt ggatttattg
    54961 gataaggaat tggctcagaa accttatatt gcaggcaatg actatacgat tgcagatatt
    55021 gctatctggt cttggtatgg acagttagtt caaggaaatc tttaccaagg ttctgcaaaa
    55081 ttcttggatg cctcaagtta tcaaaatcta gtaaaatggg cagaaaaaat tgccaatcgt
    55141 ccagctgtta agcgtggctt ggaagtaact tatacagaaa ttaaatagca aaaaagtcat
    55201 cttcggatgg ctttttgcta cagttgaggg aaagggtttg cagttatcgg cttttgtgct
    55261 ataattgcta taatatagta aaaagcaaga ggagtgtgag gaagtggaaa agaaaattgt
    55321 gaaggatatc ttatttttat ctcaagtgtc tcagccggca agtcaggagg acctttatct
    55381 tgccagagat ttgcaggata cactcttagc aaatcgtgat acctgtgttg gtctagctgc
    55441 caatatgatt ggggtgcaga agcgcgtgat tatctttaat cttggcttgg ttcccgtggt
    55501 catgtttaac ccagtgcttc tgtcctttga aggatcttat gaggcagaag aaggctgttt
    55561 gtccttggta ggtgtgagat caactaagcg ttatgaaacc ataaggcttg cctatcgtga
    55621 cagcaagtgg caggaacaga ccattacctt gacaggcttc ccagctcaga tttgccagca
    55681 tgagctggat cacttggaag gacgaattat ttaggaggaa agcaaatgaa acgaatagtc
    55741 tttgaactta tttttatcgc aacgacctgg tatatctttt taccgcccct taacctgacc
    55801 agctgggaat ttctcttctt cctctgtggg catttgttag ttgtggcaat cttatttggc
    55861 tttggcaagg ggataaacct tgtcaaaacg gttcatgtgc gccacggtaa ggcggaagct
    55921 gccttaaatc ttgagggttt caaaatcaat cggttaggga aaattctgtt agcttcgatt
    55981 ggaggaattc ttctcttggc agctttggtt tccttggtaa cttctagcat gtttcaggct
    56041 aaaaattatg ccaatgtagt cacggttacg gaaaaagact ttactgaatt tcctaagagt
    56101 gacaccagta aggttcctat cctagataga agtactgctg aaaaaattgg agaccgctac
    56161 ttgggttccc taaccgataa ggtgtcgcaa tacgtagcgg cagataccta tacccaattg
    56221 acaattgatg ggaaacctta tcgggtcaca ccactagaat atgcagaccc tatcaaatgg
    56281 tttaacaatc aagccaaggg aattggtgag tatatcaagg tggacatggt aactggaaat
    56341 gcggatttgg tggacttgaa gacaccaatc aagtattcag actcggagta ttttaaccgt
    56401 gatgtcaaac gtcacctgcg cttgaagtac ccgaccaaaa tctttaaaac tccatctttt
    56461 gaggtggacg atgagggcaa tcctttctat gtagcaacgg tttaccaaaa gcaatttgga
    56521 cttgctgttc ctcgtcctgc ttcagtcatt atcttggatg ctacaaatgg agaaaccaag
    56581 gaatacagct tatcagatgt tccagaatgg gtggacagga tctatccagc agaggaaacc
    56641 attgagcaaa ttaactacaa cggcaagtac aaggacggtt tcttgaatgc catgatttcc
    56701 aagaaaaacg tgacccagac taccaagggc tataattact tgtctatcgg taatgacatc
    56761 tatctctaca caggtgtgac gtcggctaat gcggatgaga gtaatcttgg tttcatcctt
    56821 gaaaatatgc gaacaggaga aatcactaag tatagcttgg cttctgcgac agaagaatca
    56881 gcccgtgaat cagcagaagg tgctgttcag gagaaatcct acaaagcaac cttcccaatc
    56941 ctcatcaacc tcaatgacaa gcctctctac atcatgggct taaaggacaa tgctggcttg
    57001 gtcaaagagt acgccctggt agacgcagtc gagtaccaaa atgttatcgt tgctactaca
    57061 gtggaagaga tgctcagcaa gtatgccaat aaaaacgacc ttgaaattga caatgcaacg
    57121 acagaaagca tcaagggagt agtagcagac ctcaaatcag ctgttatcaa gggcgacacc
    57181 gtctacttct ttaaagttga tggcaagatt tacaaggtta aggcctcagt atccgatgac
    57241 cttccttacc ttgaaaatgg tagaaccttc gaaggtcaag taggaaaaga cagctatctc
    57301 aagaccttta aagtacagta atactctgcg aaaatctctt caaaccacgt caactttatc
    57361 tgcaacctca aagctgtgct ttgagcaacc tgcggctag
//
LOCUS       DAVLVF010000005        47396 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVLVF010000005 DAVLVF010000000
VERSION     DAVLVF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47396
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.005"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(159..1139)
                     /locus_tag="VYN66_000262"
     CDS             complement(159..1139)
                     /locus_tag="VYN66_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV1494351.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1456..3471)
                     /gene="recG"
                     /locus_tag="VYN66_000263"
     CDS             complement(1456..3471)
                     /gene="recG"
                     /locus_tag="VYN66_000263"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV1494352.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLI
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLALIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3490..4593)
                     /locus_tag="VYN66_000264"
     CDS             complement(3490..4593)
                     /locus_tag="VYN66_000264"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV1494353.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4583..4951)
                     /locus_tag="VYN66_000265"
     CDS             complement(4583..4951)
                     /locus_tag="VYN66_000265"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV1494354.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(4990..6021)
                     /locus_tag="VYN66_000266"
     CDS             complement(4990..6021)
                     /locus_tag="VYN66_000266"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1494355.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6023..7054)
                     /locus_tag="VYN66_000267"
     CDS             complement(6023..7054)
                     /locus_tag="VYN66_000267"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1494356.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7135..9648)
                     /gene="secA"
                     /locus_tag="VYN66_000268"
     CDS             complement(7135..9648)
                     /gene="secA"
                     /locus_tag="VYN66_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV1494357.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRSLKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(9794..10081)
                     /locus_tag="VYN66_000269"
     CDS             complement(9794..10081)
                     /locus_tag="VYN66_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494358.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(10109..10741)
                     /locus_tag="VYN66_000270"
     CDS             complement(10109..10741)
                     /locus_tag="VYN66_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1494359.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            complement(10745..11386)
                     /locus_tag="VYN66_000271"
     CDS             complement(10745..11386)
                     /locus_tag="VYN66_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494360.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLVFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            complement(11379..12170)
                     /locus_tag="VYN66_000272"
     CDS             complement(11379..12170)
                     /locus_tag="VYN66_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494361.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYPGLKRKLTLQVASIPCLIYLVTVLTIAIITYFLGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            complement(12192..12584)
                     /locus_tag="VYN66_000273"
     CDS             complement(12192..12584)
                     /locus_tag="VYN66_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494362.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAADMARQNWWDLSAAISGSWSPN"
     gene            12911..13762
                     /locus_tag="VYN66_000274"
     CDS             12911..13762
                     /locus_tag="VYN66_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1494363.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
     gene            complement(13994..14344)
                     /locus_tag="VYN66_000275"
     CDS             complement(13994..14344)
                     /locus_tag="VYN66_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494364.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMIRLRRE
                     LGKSKGVHKGRCRN"
     gene            complement(14484..14819)
                     /locus_tag="VYN66_000276"
     CDS             complement(14484..14819)
                     /locus_tag="VYN66_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494365.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(14907..16217)
                     /gene="der"
                     /locus_tag="VYN66_000277"
     CDS             complement(14907..16217)
                     /gene="der"
                     /locus_tag="VYN66_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV1494366.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(16231..16944)
                     /locus_tag="VYN66_000278"
     CDS             complement(16231..16944)
                     /locus_tag="VYN66_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV1494367.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(16941..17837)
                     /gene="dnaI"
                     /locus_tag="VYN66_000279"
     CDS             complement(16941..17837)
                     /gene="dnaI"
                     /locus_tag="VYN66_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV1494368.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(17838..19007)
                     /locus_tag="VYN66_000280"
     CDS             complement(17838..19007)
                     /locus_tag="VYN66_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV1494369.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(19008..19481)
                     /gene="nrdR"
                     /locus_tag="VYN66_000281"
     CDS             complement(19008..19481)
                     /gene="nrdR"
                     /locus_tag="VYN66_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV1494370.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            19621..19986
                     /locus_tag="VYN66_000282"
     CDS             19621..19986
                     /locus_tag="VYN66_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1494371.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            19991..20686
                     /locus_tag="VYN66_000283"
     CDS             19991..20686
                     /locus_tag="VYN66_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494372.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            20703..21470
                     /locus_tag="VYN66_000284"
     CDS             20703..21470
                     /locus_tag="VYN66_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494373.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLGISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(21823..23022)
                     /locus_tag="VYN66_000285"
     CDS             complement(21823..23022)
                     /locus_tag="VYN66_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1494374.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(23019..23912)
                     /locus_tag="VYN66_000286"
     CDS             complement(23019..23912)
                     /locus_tag="VYN66_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494375.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQEIYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            24029..24109
                     /locus_tag="VYN66_000287"
                     /pseudo
     CDS             24029..24109
                     /locus_tag="VYN66_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            24619..24807
                     /locus_tag="VYN66_000288"
                     /pseudo
     CDS             24619..24807
                     /locus_tag="VYN66_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            25222..25839
                     /locus_tag="VYN66_000289"
     CDS             25222..25839
                     /locus_tag="VYN66_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV1494376.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(26159..27046)
                     /locus_tag="VYN66_000290"
     CDS             complement(26159..27046)
                     /locus_tag="VYN66_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1494377.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(27195..29078)
                     /locus_tag="VYN66_000291"
     CDS             complement(27195..29078)
                     /locus_tag="VYN66_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1494378.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGDKVKVGDVLGTFDSNKIATAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            29455..30909
                     /locus_tag="VYN66_000292"
     CDS             29455..30909
                     /locus_tag="VYN66_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1494379.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFRETGVKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRTSEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGITRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            30890..31855
                     /locus_tag="VYN66_000293"
     CDS             30890..31855
                     /locus_tag="VYN66_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1494380.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKPAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(31890..33164)
                     /locus_tag="VYN66_000294"
     CDS             complement(31890..33164)
                     /locus_tag="VYN66_000294"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV1494381.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(33164..34336)
                     /locus_tag="VYN66_000295"
     CDS             complement(33164..34336)
                     /locus_tag="VYN66_000295"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV1494382.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(34467..34715)
                     /locus_tag="VYN66_000296"
     CDS             complement(34467..34715)
                     /locus_tag="VYN66_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494383.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPISQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(34783..35408)
                     /locus_tag="VYN66_000297"
                     /pseudo
     CDS             complement(34783..35408)
                     /locus_tag="VYN66_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(35470..35919)
                     /locus_tag="VYN66_000298"
     CDS             complement(35470..35919)
                     /locus_tag="VYN66_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1494384.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(35921..36679)
                     /locus_tag="VYN66_000299"
     CDS             complement(35921..36679)
                     /locus_tag="VYN66_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1494385.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAKPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(36794..38773)
                     /gene="pknB"
                     /locus_tag="VYN66_000300"
     CDS             complement(36794..38773)
                     /gene="pknB"
                     /locus_tag="VYN66_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV1494386.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(38770..39510)
                     /locus_tag="VYN66_000301"
     CDS             complement(38770..39510)
                     /locus_tag="VYN66_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV1494387.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            complement(39525..40838)
                     /gene="rsmB"
                     /locus_tag="VYN66_000302"
     CDS             complement(39525..40838)
                     /gene="rsmB"
                     /locus_tag="VYN66_000302"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV1494388.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNALLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(40831..41766)
                     /locus_tag="VYN66_000303"
     CDS             complement(40831..41766)
                     /locus_tag="VYN66_000303"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV1494389.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHAFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(41779..44175)
                     /locus_tag="VYN66_000304"
     CDS             complement(41779..44175)
                     /locus_tag="VYN66_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV1494390.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEVAYK
                     QDSNPRYHARDVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(44241..44555)
                     /locus_tag="VYN66_000305"
     CDS             complement(44241..44555)
                     /locus_tag="VYN66_000305"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV1494391.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(44580..45206)
                     /gene="gmk"
                     /locus_tag="VYN66_000306"
     CDS             complement(44580..45206)
                     /gene="gmk"
                     /locus_tag="VYN66_000306"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV1494392.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(45336..46949)
                     /locus_tag="VYN66_000307"
     CDS             complement(45336..46949)
                     /locus_tag="VYN66_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV1494393.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(47019..47309)
                     /locus_tag="VYN66_000308"
     CDS             complement(47019..47309)
                     /locus_tag="VYN66_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1494394.1"
                     /translation="MEVEEKSIELSYEYVDDGYSGSNFERPNFKNMIDDLNKGKFSII
                     MVKDLSRFGRDYIESGKYLQKIFPEKGIRFISVNDNYDSDNADVSDIHLRFV"
BASE COUNT        13646 a        10721 c         8958 g        14071 t
ORIGIN      
        1 cattgttaga aatcgatttg actgtcctga tcgttttgtc ctattcttgt ttcattttac
       61 tatatttcca tgataaaacg catagtatca agttttttta atcccctgat actatgcgtg
      121 tttgtaattt ttaagatttt gtgcttagag tcgactcctt attttagata tttaaaagga
      181 atctcacttc cacagagcca gttgtagact tggtcattga caaatacatt catggcttcg
      241 tgagcatact caggcatgat gcgataggtt ttatcgcagg tcagacgatt ataaatcgca
      301 aactgggtaa tgggatagca aacatcgtcg tccaagcccg taatcatctt aacctcacct
      361 tggatacgat gggcaagatt tttgacatcg atataggcaa gggtcgccat gatttcctcc
      421 tctgtttcat ggaaggggtc gtgaaacttg aaataacgga aaagttcgtc gtaagcctcg
      481 ctagtattac caatctcaat cacccgtctg aagtctgaca agaagggata aatggcaact
      541 gttttctgaa ttcgaggatt gagcgctgct gcaactagag ctagagcccc tccttgtgag
      601 gcaccatagc tagaaagacg cttctcatca acctgagaca gactagcaac aatttcgacc
      661 aactggtaaa tatccagata aacatcctta taaaagaggt ggtcccgacc ttccatagca
      721 ccacggataa tatgcccctt cacggtattt cctaaaggag aacgcaagcc gtcttgtgag
      781 taacctgact ggccccgcac atccatggaa acaacaccgt aaccagctac ggtgaagccc
      841 agcatgtcgg cccagtccca gccacgtccc atataaccat gaaaatggaa gattaatgga
      901 accttctcct cactctttgg aagaacaatg cgtgcataga cctttccttc cttgcttcct
      961 tcaaatgtta actcatagca cttgacttga ggaatgtgga aatctctttc ctccaagtgg
     1021 taggatggaa gtgtggaaac atttttcact tccccatccc agaaagcatc aaagtcttcc
     1081 ggaacctcat cccttcctct ataggtctta atttcttcta gcaaagctgg atttttcata
     1141 gcatgctcct tttattttgt aatcgttacc acaactgtag catatctatg taatcaatgc
     1201 aagaaagaag ggtaaataga aagtgaattt agattcttta cttttaggat gatgggctga
     1261 aagtagtttt agaaacgaaa aaagagcatt tcgctctctc ttctgtttat actagttttt
     1321 tgcttccttc ttttggaaag tggtttggta atttacttta atcgttactg tcatatgaga
     1381 gtcctcgttt ctttttgatg actttagtat aagagccaaa cctaaaaact agcttaagtt
     1441 ttccttagag aaagcttaat ccagatgttc tttcttttcc agatgaaggg caatcatgcg
     1501 ccactctgga tcttcttgcc aagcttctat agaactaatg tagctagcaa cctttcttgc
     1561 ttcttctaaa atcggaaaat cttcgataat atcagccact tggaactctg gaagtcctga
     1621 ctgtctggtt ccaaaaatct caccagaacc acgcattttc aaatcttcct ccgcaaggac
     1681 aaatccattg gtcgtttctg tcatgatacg catgcggtct ttcccagaat ccgtcttggg
     1741 attagcaacg agaacagcgt aggactgctt gtccccccga ccgacacgac ctctaagctg
     1801 gtgaagttga ctgagaccga agcgatcggc atccatgata atcatgacag tcgcattggg
     1861 aacgttgacc ccaacctcaa taaccgtcgt cgaaaccaga atatccgtct ttctctcctt
     1921 gaaatcctgc atgatctggt ctttttcgtc actcttcatc ctaccatgta gaagagccac
     1981 ctctgccttg cctgcaaaat gagtcgtcaa ctcctctgat aaggcaatgg catttttcaa
     2041 atctagagct tctgattctt caatcaaagg agagatgaca taggcttggg aacctttttg
     2101 aatttccccc tctaaccaag tcaagacctg aggtagttgc tcatgtttga tccagcgcgt
     2161 cacaataggc ttccgacctg ctggcatctg gtcgataatg gaaacatcca tatctccaaa
     2221 ggctgtgatg gcaagcgtcc gtggaatggg agtcgccgtc atcatgagga catctggatt
     2281 gtcacctttt tcccgtaaaa tacgcctttg ccctacacca aaacggtgct gctcatcgat
     2341 aataatcaaa gcaagacgag catactccac cccatcttgt atcagagcgt gagttccgat
     2401 aatcaaatca gcctcaccct tggcaatggt ctccaagacc tctctctttt ctgcagcttt
     2461 caaggaacct atcaagagag ccagtttcaa attgggaaaa aggttctgta aactctcaaa
     2521 gtgttgctct gcgaggattt ctgttggtac cattagggca gcctgataac ctgctgtcac
     2581 tgccgcaaac atggccaagc cagcgactac cgtttttcca ctccccacat ccccttgtag
     2641 gagacgattc atgtggtggt cggacttcat atcagttaaa atttcctgca aactcttttc
     2701 ctgagcttgg gtcagggcaa aaggaagact tactttaact gctgtcactt tttcctgaga
     2761 ccaattcaga accagaccac ttccctgaac tctattttca gacttgagca tctgcagctg
     2821 catttggaaa taaaagagtt cctcaaactt tatacggcga agagcctgct tgtattctgc
     2881 caaatccttt ggaaaatgca tagctcggac tgcctgacaa cgggacatga gtttgtattt
     2941 gtctagtaaa gactggggca gattttcttc tatcaagagg tccagtccct gatcaaaagc
     3001 cgtcttgatg accttgacca gactggcctg actgattccc tgagccagac gatagacagg
     3061 ctggaggtca tcttctacct gagccagaac cttcatccca gtcagactag ccttagcgcg
     3121 gtcccatttt ccaaagacag caagggttgc tcccaactct attttatcag ccagataggg
     3181 ctggttaaag aaattcaccg caaaaacgac ctctccctgc ttgagactaa aacgcaggcg
     3241 attgcgcttg aaaccataat actggacact agcaggagtc actacctgac cagaaagaac
     3301 tgccttctca ccgtcttcca gctccagcac ctgcttggtt ttgaagtctt cataacggaa
     3361 aggaaagtag agcaagagat cttgcaagtt ttcaattcct agtttggcgt atttttctgc
     3421 tgactttggt cccacaccag gcaagacatg caagggttga tgtagattca tgctccactc
     3481 ctttcttttc taataatatt ctctcggaat acggtcgctg aggaggcaaa ccacctcata
     3541 gttaatggtt acgcggtagg tcgctacctg agttgcagtg atttccttat ccccattgga
     3601 gccaatcaag gttaccttgg ttcctagcgg ataaagctta ggcaatcgaa tagtgatttg
     3661 gtccatcgaa accctgccga caattgggca agcttggcca tctaccaaga cagagaaatt
     3721 ttgcatgtct cttgtccatc catctgcata cccgattggc acggtcgcga tgacttgctc
     3781 gctatccgct tgataagttg ctccatagcc catgcaagct ccagctggaa ctgtcttgac
     3841 atgaaccaga gcagactcca aggtcaaggc cggtatcaaa tcataaggca aatccaagac
     3901 cgctccactt ggattgaggc catacatggc atctcccata cgaaccgcat tgaaaatagt
     3961 ctctacatgc caaagagtcg ttgcagaatt gctagcatga accagctctg gaacttcctt
     4021 catactagct aaaatagttt taaaccgttc taactgggca ttaaaatagt catctgattc
     4081 ctcatcagca gtagcaaagt gggtaaagat tccttcaaca caaacaccgt gttgttggag
     4141 caaatcttga gcctgctcaa cctcacttgc ctctctaaaa ccaatccgtc ccatccctga
     4201 atcaatcttg aggtggactg tcaatccagt taggtccact tccttatcta agagtgcttg
     4261 aatccactcc agtccagcca ctgtcaaggt gaagtcatat tctttagcta gagcaacagc
     4321 ttcgatttca gaaactccta aaatgaggat tggcttgctg agtccagctt gtctgagttc
     4381 aatggcttca tcgatattgg aaacgcaaaa gccatcaaca tcatcttgaa ttgccttggc
     4441 aacggcaaca gctccatgac cataagcatt ggccttaacc acagccaact tgagcgttcc
     4501 ttgagggata tgagccccca tttgctgaat attttgtcga atagctccca gatgaatcag
     4561 agccttggtt ggtctatgtg gactagcttt catgattttc ctccaaaatg acactggctg
     4621 tcacaaactg atcggtgtgg ctgatagaca gccaaatctt tcctgaaaat ggtgcctgac
     4681 taaaataagg cgccccacgt tcattgttca agacttccaa atcctgaaaa ccgagcttgc
     4741 taatgcccgt tcccatagcc ttggaaaagg cctccttagc cgaccagcga ccagctaaat
     4801 attctatttg cctgcgtcct ttgagactgg tgaagcgctc catttcctga gcggtcagta
     4861 cacgcttagc aaatccttca tgtcgtgtaa ctgcgctttc tatcgaagcc aattcttcga
     4921 tgtcaattcc gtgtccaact atcattctca tcaaaaggag ttgagattcc ccaactccat
     4981 ccttttcact tattttgtca aggtaacata aatctcttct accaaggcct ctgtattttc
     5041 ccaacctagg caagggtcag taatagagca accaaagatc tctggttggt tttgacgacc
     5101 atctgctagg taagattcaa tcataaatcc tcgaaccgtc tttttaattt tctcattcca
     5161 atcacgattc tgcaaggtct ggcgaacaat tcgaatctgc tccatatatt gcttgcctga
     5221 gttatcatgg ttggtgtcaa tgaggataaa aggattttca agtcccatgg tttcatagcg
     5281 ttcaatggct tggagtagat tttcatagta gtaattcggc atataattgc catactcgtt
     5341 gactgctcca cggaggataa catgggccaa aggattacct gatgtctcaa cttcctgccc
     5401 atgataaagg aaggtttgct tgttttgagc agcatagatg gcgttaaaca taacacccaa
     5461 atttcctgag gttggatttt tcatccctac tggtgcatca atcccagaag ccacaaagcg
     5521 gtgctcttgg tcttccacag aacgagctcc aacggcatgg tagctgacca agtcatccac
     5581 caagatcaga tttgacggat aaagcatctc atctgccgtt gtcaaaccag tctctgtaat
     5641 cacgcggtag tgcaactggc gcacagcctg caagccatta atcaggcttg gagccttaga
     5701 agtatctggc tggtgaacta atcctttata gccgtctcca ttggtacgag gcttagcagt
     5761 atacacgcgc atgaccatga aaatcttatc cgctaccttc ttttgcaagg cagataaacg
     5821 gcgagcatat tccaagaccg cctcttcatt atcagaagag caaggaccaa tcaccaagag
     5881 aatacggtca tcttcccctg aaataatatc tgccaattcc ctgtcacggc tttccttgag
     5941 tctcaaggct tccgcagaca attgggtttc agccttgatg acttccatat cgatttcttg
     6001 acctttttca ataaatgcca tcttattctc ctaacgtttt gtaaatttct ctgacaagag
     6061 cttctgtgtt atcccaaccc aggcaagggt ctgtgataga cttaccaaat acttctggct
     6121 cattttgtcg accatcttcc agataagact caatcataaa accacgaacg aactgcttaa
     6181 ttttttcatt ccaagcacgg ttaatcaagg tctggcggac aattcggatc tgttcaatat
     6241 actgcttacc agaattgtca tgattggtat caatgatgat aaaaggattt tccaagccca
     6301 ttttctcata ctgggcaatg gtatcaatta aattgtcata atagtagttg ggaatatttt
     6361 ttccatactc attaagagca ccacgaagaa tagcgtgtga aagcgggttc ccagttgttt
     6421 ctacttcttt tcctaagaaa aggaaacttt gtttgttttg agcagcataa atcccattaa
     6481 acatgacatt gagatttcca gaggttggat ttttaaaacc agtagaaaat cctgccccac
     6541 ttgccacaaa gcggtgttgc tggtcttcaa ctgaacgggc accaactgcc atgtaagaaa
     6601 tcaaatcatc tacaagcgga aggttttcag gataaagcat ttcatcagca gttgtcatcc
     6661 ctgtttctgt gatgacacga tagtgaagat ggcgaacggc tttgattcca ttgataagac
     6721 taggcgcttc tgtcgcgtta ggctggtgaa tcaagccctt atagccatct ccgttggtac
     6781 ggggtttggc agtataaaca cgcataacca taaagatacg atctgccact tcttcttgta
     6841 ggactgccaa acgcttagcg tattcaagga cagcttcttc gttgtcagat gagcatggcc
     6901 cgattaccaa gagaattcgc tggtcttctc cacgtataat ggcttctagc tcttgatctc
     6961 gctgtgattt cctctccaaa gcctgacctt ctaattttga caaggcacga acttcttcta
     7021 tatttatttt agagcttttt gctgtaaata ccataactca tctccttata aagcaaacgg
     7081 atacccagta aaaatttact tttcacaaga tatccgcctc taaactatct cattttattg
     7141 tcttttaccg tgacagtttt taaatttctt accagaacca catgggcaaa gttcattgcg
     7201 tccaatctgg ctcaaatcca aatcttctgg catacttgct tggtgagcag cgatattgcg
     7261 agtcgctgtt gtactgatat gacgttctgc ctgtggtctt tcttgttcat gaatttgtgc
     7321 tttcatcatc aagcgtgtca catcaaactc aatcgaacca atcatatcat taaacatacg
     7381 gaaaccttct gcctgatact caacaacagg gttgttctga gcatagccac gaagtccaac
     7441 cgcgttacgc aattgatcaa gggcatcgat atgatctgtc cacttgttat ccaccactcg
     7501 tagaatcaaa actttttgga attctttaac tgcttcttca tcgcgtagtt ttgaaacctg
     7561 actatcgtaa accttcaagg aacgttggaa aagctcttcc ttgatggcct tatcagacaa
     7621 gcctgacaag tcttccatcg taatagaatc ttcaggaagc aagttgtact tagcaaagtt
     7681 caaaattgcc tctagttttt catcttgttt ggcacgcgca tgaccatcaa cgacacgttc
     7741 aatcgtgcgt ttgatcatag actgaatttc aggtgccaag tcacgatctg cagtgatgac
     7801 atcgtaacgt tgagcataga taatctcacg ttgttcacgc atgacatcat cgtattgaag
     7861 gacttgttta cgggtatcgt agttatttcc ttcgacacgt ttctgagctg cttcaacctg
     7921 acgcgtcaac atgcgagact caatggcctc ttcagacatg ttgagacgtt caaagattcc
     7981 cttcaagcgt tcagaaccaa aacgtttcat caaatcatct tcaagagata ggtagaattg
     8041 tgactcacct ggatctcctt gacgacctga acgtccacga agctggttat cgatacgacg
     8101 actttcatga cgttctgtac caataacaca aagtcctcca agttcacgaa caccttcacc
     8161 aagcttgatg tcggtaccac gacccgccat gttggttgcg atggtaacgg caccacgttg
     8221 accagcattc atgatgattt gggcttctct atagtggttt ttggcattca agacttcgtg
     8281 aggaacacca gctgcaacca atttcttaga aatgtagtca ctagtttcaa ccgctactgt
     8341 accaaccaag acaggttgac ccttttggta acgagcctta acgtcttcga caaccgcttt
     8401 aaacttagat tcgatacttg cataaagaag gtctgagtgg tcaatacgtt gaacaggacg
     8461 gtttgttggg attggaataa cacgaatgtt gtagatttca cgaaattctt cttcctcagt
     8521 cttacctgta cccgtcatac cagacaattt tttgtacata cggaaaaggt tttggtacgt
     8581 gattgaggca gatgtcttgg tttcatcctg gattggcaca ccttctttgg cttcaatagc
     8641 ttggtgcaat ccatcagaat aacgacgacc ttccatggta cgacctgtaa attggtcgac
     8701 aatcaagatt tcttgctctt cgctcaccac atagtcaata tcgagaagca tgatgtagtt
     8761 ggcacgaagg gcgttatcga taaagtgggt caaagccacg ttttcgatgt catagaggtt
     8821 ttcaagtttg aagtagcttt cagccctgtc aatccctgaa tcagacaaac caatagtctt
     8881 agactgcaca tcgatgatgt agtcatcttt gttcaaagat tttacatagt ggtctgccat
     8941 gtgatacaac tgactggttt caaccgcatt ggcacctgat acaatcaaag gtgtacgagc
     9001 ctcgtcaatc aagatagagt caacctcatc gaccaaggca tagttaagcg gacgttgtac
     9061 catgttttca gcgcgaacga ccatgttgtc acgaaggtag tcaaatccga tttctgagtt
     9121 agttgagtaa gtaatatcac actcataggc ttcttttttc tccattggag atttggtagc
     9181 caagttaatc cctactgaca aaccaagcca agagtacaat tcacccatct cagtcgcgtc
     9241 acgttctgac aggtattcat taaccgtaac tacgtgaacc cctttacctg aaagggcatt
     9301 gaggtatacc ggcatggtcg cagttaaggt tttcccttcc cctgtacgca tctctggcac
     9361 gtcaccatgg tgaagaacaa tcccccccat gacctgaacc ttataaggga agagacctag
     9421 gacacgtttg gcaccttcac ggacaaccgc aaatgcttcg taaagcaatg aatccagtga
     9481 ttctccattt tgataacgtt ccttaaattc aactgttttt gcttttagtt ggtcgtcagt
     9541 caaagcagcc atttggtctt cgtatttgaa aaccttgtca gccatctttt ccagacgacg
     9601 gatttctcct ttatcatttt cgataattgt ttttaaaata ttagccatgt ttttccttac
     9661 tttaaattcc gaatatttta gaatgttcct ttaattttag cacaattact gattattttc
     9721 aaggaagaat ccccattttg aaaaggaaaa agaggttagt tttcaagcta acctctccga
     9781 cttttatgac tgtttaattg ccaaaaatcg gcaaaatggc aaatgtgata aataagttaa
     9841 tccaaagaga aagacagcaa atcagtccaa aaacaaagag actgactttc ttggacagca
     9901 aggccatgag aatggacaga attccaaaag ctagcaagat tttctgtatg atgaagatgt
     9961 caatccttac ccccagaatc gctctatccc aagggataaa gaagaaagac agccagatca
    10021 gcagaactaa accaatataa accttggaat agcgtgtaat aaatgatttt agatgtgcca
    10081 taagccatct cctaggattg tatatccttt acatttgtat atgtccattt tcgacaacta
    10141 atacatctgt acaaaggtct gaaatatctt ccgatatgtg cgacgtcagg ataaccagac
    10201 cattttcttt tttcaggtaa gataaaatga cctgtttggt taacctcaca ctcttctcat
    10261 ccaaagcatt cataggttca tcgagaaaga gtatagaagg agaggaaata aaggcttgaa
    10321 tcaaggccat tttttgcttt gttcccaagg ataaatgacg gtattcaacg tcttcaaatt
    10381 cctgtaaatc atagtattga atccaatagg caattctttt ttccgtaact ttagatgaaa
    10441 aataccttaa cagttccaaa ttttctctca gggataaatg agataaaaac tcgacttttt
    10501 caattaaaat tcctgcatcc tgaatataat gattttttat cccgtagact ttcccgtctt
    10561 gaagaacttt tcctttgtca agcttaatat agccagcaag tacctttaaa agaaccgtct
    10621 tgccagatcc attatcacct ttaagtccat aaatttttcc tgattgaaac accaaattca
    10681 actgatttaa aataggtctt ttcccataaa ttttctgtcc atttataatt tcaagtctca
    10741 tcgtttatcc tttcttaaat ttctcttcca agcgactaac aatcaacata actaataaac
    10801 ttaagagtag taaatacact gttacaatat gatccatcat ccattcttgt cccacaaata
    10861 ctaaaaaata gagaaaaaag gaaaaaccag cgcccgcact aatcagtgct atcagataag
    10921 acagtagtaa gtacaacatc attaactgag caagtaaaaa gaaaagtgcg actgttttga
    10981 aatgaattcc tagcggaagt aaaccaagga accagacaaa caaatcttga gtaaaaaatt
    11041 tagaaatgac aaccaatcgt ttccttgtat aatcgaaaaa ggagcttgat tgatagcgaa
    11101 tcaagtgatg gtaatggtct gccaaatcac tttcaagtaa aatgacagag agccatccta
    11161 ccatgaaaag cgaaaggttt aacaagagtt tggggatttc taaattcgct tcagaatagt
    11221 cattatagga aaaaattgaa atcaattctc tcttgaaaaa gataacagca tcctgctttt
    11281 gaaccgctgt tgacaataga tggtttatca aaatcatcat cagaactata cgaagtagta
    11341 aaacaatctt tctctttagc aaaatattgt taaaattctt aaacattatc ttcatatttt
    11401 tcaagcacca tgtaagggac aatataaagg atataaggag taaagagttt catcaaactt
    11461 acatccccat agctagcttg catcaaactc gtcataggaa ccatatagta aggcaaggca
    11521 ctatagagca gcatagaacc aagccaaaga aacatcaaat aggtgattgc cgaacgagtc
    11581 acattcccca cataatcaac tatttgtaaa aaatagattg cattgatgaa aataccgatt
    11641 agcaggacac aagtaaagaa caaatagctt tttatctctc catctaggag tctttgtaaa
    11701 ccacttccat cagaaaatag agaattccat ccaagaggag aaaaagtccc aaggaaatag
    11761 gttataattg caattgtcag cacagtcact aaatatatca aacaagggat acttgcaact
    11821 tgcaaagtca actttctttt taaccctgga tagctcactt ctcttccaat actcaatcgt
    11881 aaaactttat ttttcagctc aatatattga aaacctagca aaacaatgat gattggaata
    11941 ataaatgatt gataaaaacc tagattgaac tggagaatac gaaaatctaa tgctgatttt
    12001 aataagtcat ttttaattgc ttgtccatcg aatttagcta gatcgggcgc atgaatatca
    12061 aaaaagaaat aagtcatcaa aacgtcacga taaaccaaag caagaaaaag caaagtaagc
    12121 aagacataaa caaatttatg ccttgccaaa gttaagagag gaatcttcat aaattatacc
    12181 tactttacga attaatttgg tgaccaacta cctgaaatgg cagctgataa atcccaccaa
    12241 ttttgtcttg ccatatctgc tgcataaaca tagccaaccg ttgcccaatt accatgagtt
    12301 gaacgagtac cacaggttgt taacgattct ccactacgag cttctccatt agaatttact
    12361 atacgatgct taacagtcat gggatgtaaa tttctacaag gatttaagtt catgatatat
    12421 tccgcacctt cattttcttt agtcaaataa cctgttctct tataactggt agtaataaca
    12481 aaattactaa aagcacctat ctgatttgct tctacgaaag tagttccaac agcacttatc
    12541 attaaaacag gaataaccat tttcaataat ttcttaattt tcattttttc tgtttccttt
    12601 cattttttat tcaagaagat tccttataac ttctatttta aatatagact tattctaaac
    12661 gatggtatat catacaaatt cctcctttac ttattatata tagactctaa tttctttatc
    12721 cccaaaacca tgtttcaaac cagataagtt gaatccaact ccaaatcaaa aatccagagt
    12781 ttttcggtaa attcttcaaa tattttttaa agcccatgac tttttccctc ctttttgata
    12841 taataatacc acctttttct acgtttttta taaatgtaga aaatattcac atttacctag
    12901 gaggaacaat atgagatggg attatggaca aatttttaaa gaaattcgaa agtcaaaagg
    12961 attgacccaa caagatgtat gtggacaagt catacatcga acaactctaa caaatattga
    13021 acacggtaaa gttattccca gttttgaaaa catggtattt cttcttgaac aaattgatat
    13081 gagcttggca gaattcaagt atatatgcaa cgaataccat cctagtaaaa ggcgagatat
    13141 tattgtagag agccaaaatc cgtctacttt tcaagatact agaaaaatgg ttgaactcac
    13201 tgagaaatgt caaaaatatc ttaagacaca tcacgatgtt cctattcaaa atatctatcg
    13261 tcatacaaaa attgtcacag agttacgaac taaaggattc aaaaacaacc acgtcttgaa
    13321 agatttgtat gaagaaattt gggactatct tgaacctatg gatacatggt acattagtga
    13381 tttgaaattg cttggaacca ttctcttttt ctttccttct gaaaatcttc cccttcttat
    13441 tgatagaatt atgaaaacca tcgagaaata taaatacttc cgagaaacaa aagcattttt
    13501 atcatctttt ttagccaatc tctccactgt atattttcaa catcatttat tcaaagaatg
    13561 tgaaacaatc accctacaac ttttggtatt agcagaagaa ttaaaaatat acgatattct
    13621 tggattctct caagtacgct tgggaatact acaacataac tccgatttga ttgataaagg
    13681 aataacttta ttacgactaa ccaaagaaga ggcattagtt aaaatactcg aaaaagaaat
    13741 taatgatttt tcaaacctat aatttgtaat aactctcatc attttattat agtagattgt
    13801 aatagaatgt aatagaatgt gaacgaatca attaggggat ctaaagcatt ttataccaat
    13861 gttttataag ttgtattgta ttattccata ttcaatccac tatacaagat gaatatttct
    13921 ctcaagtttc ataatcaaaa taaaaatgac tgtatctata gctccataaa caaatcatag
    13981 atacagtcat tttttagttc ctacaccttc ctttatgcac acctttagat ttccctagtt
    14041 cccgcctcaa gcgaatcatt tttttgaaaa agacataggc taaccaatca ctagcaaaag
    14101 cgataagtag taataatagc caaatacccc ataacttgat atctgtcaaa tttctcaaga
    14161 cgatattgaa aaacagaact gaaaaaactg tccaagcaag gctaaaaaga gcatagaggg
    14221 tgatgtaaat ccagtaaaaa tagtaaaaaa ttgggaaaaa tttactttct ctattatcct
    14281 tgtcaatcca gctgaaaaag taaaaacagg gtaataagat tatcatttta aacaagtgtt
    14341 ccatcacaag cctccttttt taaattgttt tctttagttt cgttctatta aatgttatta
    14401 ccaatataaa ccttggaata gcgtgtaata aatgatttta gaggtaccat aagccacctc
    14461 ctacaaatag aaaccgatat aaatcaatgc cttccaccct tagacttccc tagttcctgt
    14521 ctcaagcgaa acatttcttt gaaacaggaa taagttaacc aattcatacc aatagctagc
    14581 agaataaaaa gaaaccaaat gccccataac ttgatatctg tcacatttct caagacggta
    14641 ttgaaaaaca gaactgaaac aactgtccaa gcaaggctaa aaagagaata gaaggggatg
    14701 taaaaccagt aaaaataata aaaaattgga aaaaacttac tatttctgtt ggccttttca
    14761 atccagttat caaaataaaa gtacggtgct aaaagtaaga atttaaacaa atgttccatc
    14821 accgacatcc cccttctttt gatagcgttt tctattattt tattatatca aaaaaatccg
    14881 gaactgtcat tccagattct acttttttat ttgcgttttc ttgcgatgag atgaatcggt
    14941 gttccctcaa aaacaaaggc cttgcggatt tgattttcca agaaacgcag gtaagaaaag
    15001 tgcatgagtt cttcttcatt gacaaagatg acaaaggttg gtggtttggt tgccacttgg
    15061 gtcgcataga aaatcttgag acgttttcct ttgtctgtcg gtgttgggtt gatggcaatg
    15121 gcatccatga tgacatcgtt caagacagct gatggaatac gtgtattttg actttcgctg
    15181 atttgcttaa tcatctcagg aagtttgtgg agacgttgct tggttaaagc tgatacaaag
    15241 ataatcggtg cgtaaggcag gtattggaac tgctcacgga tatcttcttc ccagtttttc
    15301 atagtgtggt tatctttttc aagcgtatcc cacttgttga ccacgataat catcccttta
    15361 ccagcttcat gagcaaatcc agcgatacgc ttgtcgtatt cacgaatccc ctcttccgca
    15421 ttgatgacca ttaagaccac atctgaacgg tcaatagcac gcatggcacg catgacagag
    15481 tacttctcag tattttcata aaccttacca gacttacgca taccagccgt atcaatcatg
    15541 gtaaactctt gaccatctgt atctgtaaag tgggtatcaa tagcgtcacg agttgttcca
    15601 gcaacaggac tggcaatgac acggtcttct cccaagatag cattaatcaa gcttgatttt
    15661 ccaacgttag gacgaccaat caagctaaac ttaatgacat ctggattttc ttcttcatat
    15721 tcatttggaa ggttttccac aatcgcatct agtacatccc ctgtaccgat accatggaca
    15781 gatgagatag gcagtggttc acccaaaccg agagcataga aatcatagat atcatttctc
    15841 atctcagggt tgtccacctt gttgactgcg aggataactg gtttgtgggt cttataaagc
    15901 ttacgagcta cgtattcgtc tgcatcagta attccttcct taccagacac gacaaaaacg
    15961 ataacatctg cttcttccat ggcaatttct gcctggtgct tgatttgttc catgaaagga
    16021 gcatcgacat catcaattcc tcctgtatca atcatgctaa aagaacgatt gagccactca
    16081 cccgttgcat aaatacggtc acgtgtcact ccttcgacat cttctacaat ggagattcgc
    16141 tcaccagcga tccgattaaa tagggttgat ttcccaacat tgggacgtcc tacaatggca
    16201 atagttggta gggccataat ttctcacttt ctacaataat ttcttctgtt caagattttt
    16261 tctagttgag cttggttcag cttgaccaaa ctgttctgct aggcgctgac tccagcttgt
    16321 ggtcgcacgc gccccagcat agtcagcctg aacacggtca taagcttgga ttgcctcagt
    16381 tgactgttct tggtattctt cctcaaagac aacattctct agtggcagtc tcggtttcat
    16441 atcatgatgt tgatttggca cacccagtgc catcccaaag acagaatagg tgtagtcagg
    16501 taggttaaag agctctgcca cttcttcaga cttgtatcga accaaaccga taatcacacc
    16561 accatagccc aagctttcag ctgccaacaa ggcgttttgt ccagcaagag ctgcatcgac
    16621 cgaactaatc aagagacctt ccacaccttg gggttggaag gtgtcggtat gaagtctggc
    16681 tcccttttct gctcggttca aatctccgac aaagagaagg aaaacagcag actggcgaat
    16741 ggcttcttga ggtaccaatt catacaaggc atctttcttc tcttgacttc gtaccacaat
    16801 cacagagtag gattggaaat tcttccaaga tgatgccatc tgggctgctg tcaaaatctc
    16861 atttaagtct acttggggaa tttcttgctc tttaaacctg cgcactgaag tatgagcctt
    16921 catcaattta atggtttctg tcatcgacgg tttactcctt ctaaacgagt ctcctcagcc
    16981 aaataacgga tgcgttccat gacccgtctg gcttcccagg tttcgtcatt tccatgtttc
    17041 actttcgcaa aatgcttctc caaatcttca aagttgaagt tggatgtgaa aaaggtcggt
    17101 aaattttcct gcatccgata ttggagaatg acctgcagga tttcatcacg cacccaagcg
    17161 gtggattgct cggcgccaat atcatctaaa atcaggacct cagacagctt aatctcatcc
    17221 accaaggtct taacattgcc atcactgata gcatttttga catcaatgac aaagctagga
    17281 tagtggagga gagttgatga aacaccacgt ttttctgata aatcatgagc taaggccgcc
    17341 accatgaaac ttttacccac accaaagtct ccatataagt aaagaccttt tcgaatagct
    17401 ggatattgct ccacgaaggc tagtagcttt tcaaaaactg gtaagcgccc caaatcatcc
    17461 aagtcaactt gagccaaact agctttcttg agactggctg gtagattgat taacttgaga
    17521 cggttcttaa tagccgcttc tttttcagcc gcgattagct caggagtttc ttcatatgaa
    17581 acatctgcat agccatgatt cttaaccaaa atcggcttgt agcctttggc aatataatcc
    17641 gtatccccac ggagaaactt gtcacgctcg gtgatgtact gattaaactt ggagatactg
    17701 cgatttaatt cctctggagt caaggattct tgctggataa aagccgcaac atcagggtcc
    17761 ttcatgattt tctggaccaa atcttgataa taaaaacggc tgggttgacg tttgagtacg
    17821 tctccgacac tttccatcta atctcctcct ttttctaatc gagctaatag ttcttgcttc
    17881 ttacgttcta gttccagacg agtttcctcg ctggtttcat tcttatattc aggattactc
    17941 catttaggaa cattggtttt tactggggca gtctgattct gtttttgtgt ttttgctttc
    18001 tgtcctcgct cacggatgcg caagactgcc ttttctgccg aatgaatctt ttgataggca
    18061 tagtcattgg ctaccttcat ggcatatttc tcattgatat ttgccgaatc gaccttatta
    18121 aaggtcaata aaagaatgat attgatgact tcgtccagca agcccaagcc agccatctgt
    18181 tgcaagagtt ctctttctgt ttgggtaatg gttcccttgc gtgtttgctt gatttctgcc
    18241 aagaactgca gggcagtttt acttttggct tctttgataa tggttgtttc cttaagacta
    18301 aagtcagagg aaactggttt ttgagcaatt ttttcacgca tgcgtttggt tgaaataacc
    18361 tgagaaacag ctgttgactt ggccaattga taggtttcaa accaagtcca tttcttctcc
    18421 tcggcaatag caaagaggtt taagacatcg gactgctcat ccgcaaaacg aagtccatct
    18481 cgagccatca gctggcgaaa atgttccaag tcaaaatcat tggccacttt cttcttgaga
    18541 ccaaggtctt cttgactgcc tagttctgcc aattctggaa agacttgatt gagtgagaca
    18601 ggtatttctt caccatcagc actttcaact ttcaaatcct ccacagcaga gtcgccaatc
    18661 tttttctcta agagtctgcg ataaacagga tgctccaaga agtcttgact agatagagga
    18721 gcatggaggg ctagctgata aacatccccc ttttgataga gggtcaagag attaaaagca
    18781 gataagattt tcaatgattt tatcagtcta tccatcccaa agttgagatg attgaggata
    18841 cttgaaaaaa gatattcctt tctaccatta tcccaaaaac tgattgtata aagataaagg
    18901 ctcagtgcct cctgaccgat aatcgggagg tagcactgta ccagagatga ggtatcttgc
    18961 gacacccgat tattctttag ataagaaaaa cggtcaattg gcttcattta tctttccttt
    19021 ttctttttag aggactgggt gatttgttgg agcaagctct ctaactcact gacatcctta
    19081 aaactacgat agacactagc aaaacgtaca taggtaatct cgtccaattc agccaactcc
    19141 tccatgacga gtgaaccaat gtcctcactt tgaatttcat tttcatttcg accacggagt
    19201 ttctgttcga tacgattgac taccatgttg atttcatcac ttgacacagg acgtttctgg
    19261 gctgagcgga taatcccatt aaagatttta tctctggaga attgttcccg tgtgccatct
    19321 tttttaacaa ccactaaggt tctttcttct actcgttcgt aggttgtaaa acggtgttgg
    19381 cactcgtcgc actctcgtct tctacgaatg gtgttccctt cttctgcttg gcgactatcg
    19441 ataacacttg acttggtagc cccacatttt ggacaacgca tcctttccct ccttatcgtt
    19501 ttcttttcat tataccattt tttaagcgat tcccaaaaca attcttcttt ttgcttgaca
    19561 agttttttgt tttgttgtat tatttaatta agacaaaagg ataagagaaa ggagactaag
    19621 atgtcctgga catttgacaa caaaaaaccc atctatttac agattatgga gaaaatcaag
    19681 cttcagattg tttcccatac actggaaccc aatcaacaac ttccaaccgt gagggagcta
    19741 gctagcgagg ctggtgtcaa tcccaatacc atccaaagag ccttatcaga ccttgaacga
    19801 gaaggatttg tctacagcaa gcgaacaact ggacgatttg tgactaagga taaggagcta
    19861 atcgcccagt cacgcaaaca attatcagaa gaagaattgg aacacttcgt ttcctccatg
    19921 acccattttg gctatgaaaa agaagaacta ccaggcgtag tcagtgatta tattaaagga
    19981 gtttaagcct atgtcattac tagtatttga aaatgtatcc aaatcatatg gagcaacacc
    20041 agcccttgaa aatgtttctc ttgacattcc agctggaaaa attgtcggcc ttcttgggcc
    20101 aaacggctca ggaaaaacaa ccctgattaa actaattaat ggcctcttac aaccagatca
    20161 aggacgtgtc ctcatcaacg acatggaccc aagcccagca accaaggccg ttgtagctta
    20221 tttgcctgat acgacctatc tcaatgagca aatgaaggtc aaagaagccc taacctactt
    20281 caagaccttc tataaagatt tcaatcttga acgcgcccat catctacttg cagacctggg
    20341 cattgatgaa aatagtcgtc tcaagaaact atcaaaagga aacaaagaaa aggttcaact
    20401 gattttggtt atgagccgtg atgctcgtct ctatgttttg gacgaaccca ttggtggggt
    20461 ggatccagca gcccgtgctt atatcctcaa taccattatc aacaactact caccaacttc
    20521 taccgttttg atttctaccc acttgatttc tgatatcgag ccaatcttgg atgaaattgt
    20581 cttcctaaaa gacggaaaag tcgtccgtca aggaaatgta gatgatattc gctacgagtc
    20641 aggtgaatcc attgaccaac tcttccgtca ggaatttaag gcctaagcaa aggagattat
    20701 ttatgttttg gaatttagtt cgctacgaat ttaaaaatgt taacaagtgg tatttagccc
    20761 tctacgcagc cgtgctagtc ctttctgccc tcatcggaat acagacacaa ggctttaaaa
    20821 atctacctta ccaagaaagt caggctacta tgctactttt tctagctaca gtctttggtg
    20881 gcttgatgct tacacttggg atttcaacca ttttcttgat tattaaacgc ttcaaaggta
    20941 gtgtctacga ccgacaaggc tatctgactt tgaccttgcc agtttctgaa caccatatca
    21001 tcacagccaa actaatcggt gcctttatct ggtcattgat tagcaccgct gtattggctc
    21061 taagtgctgt tattattctg gctttaacag ctccagaatg gattcctctt tcttatgtga
    21121 ttacatttgt agaaacacat ctccctcaga tctttcttac aggtatatcc ttcctactaa
    21181 atactatttc aggaatcctc tgcatctacc tggctatttc cattggacag cttttcaatg
    21241 aataccgtac agcactcgct gttgcagtct acattggtat ccaaatcgtc attggattta
    21301 ttgaactttt cttcaatctt agttctaatt tctatgtcaa ttcactggta ggactcaatg
    21361 accatttcta tatgggagca ggtatagcca ttgttgaaga actcatattc atagctatct
    21421 tttatctcgg aacctactac atcttgagaa ataaggttaa tttgctttaa ataattttta
    21481 cctagatatg taacatactc atagaacaaa agagaccagg caaaaagtct ttacaattag
    21541 aaaacgcata gtatcaagtg ttaaaaatcc ttgatattat gcgttttttg tgagctgact
    21601 tatttccttt cactatatcg caaaatgaaa taagaacgga acgatgggat tttggaattc
    21661 aaatcaattt ataagaatgt tttagaagta atattatcct attccagatt cagttcacta
    21721 tacaattgag ttttcaagca acctgtttac ataatgtgta cataattagg ttcgtgattc
    21781 cacccttttc acctttaaaa acctcgcttt cgcaaggctc ttctatttat aagataaggc
    21841 acgtttaaag gttttccaaa tccctaaatc atccgtttga agaacgagac tagcatacat
    21901 gcgtccgata aatcctgttg ctaccaccgc aaaaatcact gtaatagcaa gtgaaatcca
    21961 tgcttccgct ccctccgcat agccattaat cgttcgaaac ggcataaaga aggtcgaaat
    22021 aaagggaata taagaaccaa tcttcaagag gagattgtca ccagctgcac ctagagctgt
    22081 cactccaaaa aaaccaccca taatcaaaat catcaaaggc gacaaggctt tccctgagtc
    22141 ttcaggacga gaaaccatag atcctaggaa ggctgccaag actacgtaca tgaaaagact
    22201 gatcaaaata aagagcaagg tattcagtga gatagcatct cccaagtgat ccaaaatacc
    22261 agactgagcc aagaatggca aatctttaaa gagcaaaacg gcagccagac cacctacaac
    22321 atagatccca atatgcgtta aaatcactag aaacagagcc atcatccgcg catagaaata
    22381 gtgacttgcc cttatgctag aaaagacgac ttccataatt ttggtgcctt tttcactggc
    22441 aacttcctga gctgttacac ccgcataggt aatcagaatc atataaagaa agaatcctaa
    22501 ggcacctgct gcaattgttt gaataaactt tttattttcc ttggcttcat caatcttttc
    22561 tgtgaattga attgtctgcg ctaagcgttt ttcctgctct tgagacaagg aagcagttga
    22621 acgattaagc tgattttgca gttcattgag tgtacctgta acctcaaatt taattccatt
    22681 ttcaagcgat gtttcgccat gataaactgc ctttagaaca ctatcttctt gatcaatggt
    22741 caaataacct tttaattttt cttctttaat tgcttctttg gcacttgctt cgtctttata
    22801 gtcgaagtta acaccattta cattcttcag tccttctgct acagatggca ctgttgtcac
    22861 tactgccact ttattatttt tagccataga agaaccttgg agatgcccaa ttcctacaga
    22921 gattcctaaa aagaggaacg gcgaaatcac cataaagaag aaactccatg actcgacatg
    22981 tcgaagatag gtttccttga ttacaaccca catatttctc atacttccac tcctgattct
    23041 agtttaaaga tttcatcgat agttggcgct tgttggtcaa atgttgcgat atattgacct
    23101 tgagtcaaga ttgggaagag ttcccttcca gcgctctcat cctccaaaat caatttccaa
    23161 ctgccttgtt tggtcaagct cacctgtttg acatgaggaa gattttccaa ttcttccttg
    23221 cttcgttcac ttgaaacaaa gagacgcgtt ttcccgtatt gattgcggac atcctgaact
    23281 ggtccgtgca agaccacacg gccatctcga atcatcagaa tatcgtcaca aagttcctca
    23341 acattggtca tgacatggtc agaaaagata atggttgctc cgcgctcttt ttcctgaaaa
    23401 atgacttgtt tgagcaattc tgtattaact gggtccaatc cactaaaagg ctcatccaag
    23461 ataatcaggt ctggttcatg aatcagagta ataatgagct gaatcttctg ctgatttcct
    23521 tttgacagac tcttgatttt atctgtcagc tttcctttta cttccaacct cttcatccat
    23581 tgagggagtt tttctttgac ttctttggca tccatgcctt ttagagtcgc caagtagcga
    23641 acttgttcaa gaactgtcaa tttaggcatg agactgcgtt cttcaggcag ataaccaatc
    23701 cgagcataga tctcctgacg aatatcctga ccatccagac cgatttctcc ctgatattct
    23761 aggaatttta aaatactatg gaaaatcgtt gtttttccag caccattttt tccgactagt
    23821 cccaaaatac gacctggtcg cgcttgaaag tcaataccaa acaaaacttg cttggatcca
    23881 aaacttttct ctagacttct tacttctagc atctttcacc tccgaaattt cttgcactca
    23941 ttatactcct ttttgatagc ctttacaatg ttttttgtcc atttttagaa gactattgct
    24001 gtgtaaaata tggcctggag cacttttata ctcaatgaaa atcaaagagc aaactaggaa
    24061 gctagccgta gactgctcaa agtacagctt tgaggttgca gataaaactg acgaagtcag
    24121 ctcaaaacac tgttttgagg ttgtggatag aactgacgaa gtcagtaact atacgtacgg
    24181 caaggcgaag ctgacgtggt ttgaagagat tttcgaagag tattagtgat aaatccatta
    24241 tacagcagca aacttaattt ataccttccg ctcctcaact gtctattttt aatcctgaat
    24301 tgttatttga gtaactcctt tttcctcgta aagttttctt cctctaaaac ttctggaaaa
    24361 aggctaatag tttcagacaa catttttata agaaacaagt tcatctgtca tttcaagaag
    24421 gagtaatcct ttatctacta atggacggaa cagaattcaa ccgcttgtcc gatatgtttt
    24481 ctaaggatta tatagtaaaa tgaaataaga acaggacaaa ttgatcagga cagtcaaatt
    24541 gatttctaac aatgttttag aagtagatgt atactattct agtttcaatc tgctatatct
    24601 attatgcaca cccctatagg atctaatgaa aatcacaaca ggctcattca tagatagtta
    24661 cctcaggaaa ctaagaaaac gactacaaag gaagtcgcat tcatcgaaaa gtggattaac
    24721 aactatccta aaaaatgctt gaactacaag tcccccagag aagacttctg gatgactaac
    24781 ttgaacttga aatttagcaa taattaattc accatctaac tatatttagt aattatttca
    24841 gaactgatta atattaaaat taactaacaa ttcaaaggat tcatactagc cataaattac
    24901 gtccatcaga gagagactct tactactttt agattttagt ctttctagct tcagaataca
    24961 tctaaacttt agggaaaatg actattcgaa agcgcgaatg cctcaaaatt atctcagata
    25021 agctattcga aacttagaat gcttttaaat ttatggaatt gcgattattc gaaacctaga
    25081 atgcatataa cctttagttg acagacctat tctaagtctc gaagggctat ttactttcta
    25141 ttccttatca aaaaagactc attccccctt tctcctccaa aatatggtat agtagaaata
    25201 tactatctat gaggagttta catgtcacag gataaacaaa tgaaagctgt ttctcccctt
    25261 ttgcagcgag ttatcaatat ctcatcgatt gtcggtgggg ttgggagttt gattttctgt
    25321 atttgggctt atcaggctgg gattttacaa tccaaggaaa ccctctctgc ctttatccag
    25381 caggcaggca tctggggtcc acctctcttt atctttttac agattttaca gactgtcgtc
    25441 cctatcattc caggggcctt gacctcggtg gctggggtct ttatctacgg gcacatcatc
    25501 gggactatct acaactatat cggcatcgtg attggctgtg ccattatctt ttatctagtg
    25561 cgcctatacg gagctgcctt tgtccagtct gtcgtcagca agcgcaccta cgacaagtac
    25621 atcggctggc tagataaggg caatcgtttt gaccgcttct ttatttttat gatgatttgg
    25681 cccattagcc cagctgactt tctctgtatg ctggctgccc tgaccaagat gagtttcaag
    25741 cgctacatga ccatcatcat tctgaccaaa ccctttaccc tcgtggttta tacctacggt
    25801 ctgacctata ttattgactt tttctggcaa atgctttgac acgtaaaaaa tccgtttggt
    25861 ttcccaagtg gatttttaaa gcgtagatta actatagctt gatactaaat atactttggt
    25921 atggaaatca tgcatatttt tcgatagtga agcgaggact tacctagcct ttccaccgtg
    25981 atagaaacac ctgaaatcta atggtttcag gtgttcggaa actttgagcc tagtgtctca
    26041 aagtttaggt atggaatttc gaagaaagtc gctaccgtcc gtaatcactt aaggaaaggc
    26101 tcaaaaatat tgttttcaac cacaaaatcc gtttggtttc ccaagcggat tttgtgcttt
    26161 attttgaaac ttcttttgca agaacaaagt tcccaagtgt ggcagaacca tttcctgcga
    26221 ctgctggcgt cacgatatag tcacgcacat ctggtactgg taggtaacca ttaagaagag
    26281 atgtaaattt ctcacggaca cggtccagca tatgttgttg agccatgacc cctccaccaa
    26341 agacaatcac gtctgggcgg aaagtcactg tcgcattaac cgcagcttga gcgatatagt
    26401 aggcttgaac atcccaaaca gggttgttga gttcaatagt ttccccacgt acacctgtac
    26461 gagcttccaa acttggacca gctgcatagc cttccagaca tcccttatgg aaaggacaaa
    26521 cacccttaaa ctctttttca atatccattg ggtgtctagc aacataataa tgacccattt
    26581 cagggtgacc cacaccaccg ataaactcac cacgttggat gacgcctgca ccgatacctg
    26641 taccgattgt gtagtaaacc aagttttcga tacgaccacc agcattgtta cgggcaacca
    26701 cttcaccgta agcagagctg tttacgtctg ttgtgaagta cattggcacg tttagggcgc
    26761 gacgaagggc accaagcaag tccacatttg cccagtttgg ttttggagtc gtcgtgataa
    26821 agccataagt ttttgagttt ttgtcaatat caatcggccc aaatgaacca actgcaagac
    26881 cagcaaggtt atcgaatttt gagaagaact caatggtttt atcgattgtt tcgattggag
    26941 ttgttgttgg aaattgtgtt ttttctacaa cgttaaagtt ttcatcaccg acagcacaga
    27001 caaactttgt accgccagct tccaagcttc catataattt tgtcatgata aacctcttgt
    27061 ttttattttc tttattatag catacttcga aagtctaaat gtctctattt tttagatttt
    27121 cctctgtaaa tcttactatc taataaaaac gaacaaacat gtcatttgtt cgttttcaca
    27181 ttagagagga ttgattagat tttcacttcg atcacagcat cccccttcgc aactgaacct
    27241 gttgcgactg gagctactga agcgtagtca gctgtatttg taacgataac cattgttgta
    27301 tcatcaagtc cagctgtagc gattttattt gagtcaaatg ttccaagaac atcgccaact
    27361 ttcaccttat caccttgagc aacttttgct tcaaaaccgt caccgttcat agatacagtg
    27421 tcaataccaa catgaatcaa aacttcagca ccatttcttg ttttcaaacc aaaagcgtgc
    27481 cctgttggaa aggcaattga aacttcagca tcagctggtg catagaccac gccttggctt
    27541 ggtttcacag cgataccttg tcccatagct ccacttgaga agactgggtc attgacatca
    27601 gcaagagcga caacatcacc gacgatagga gttacaagtg tttcattttg aagagctgct
    27661 ggcgcaactt cttctttttc ttcagccact tcagcttgtt ttgcagctgc agttgcgtct
    27721 acttcatctt cgtaaccaaa catgtaagta agagcaaaac caagggcaaa tgatacagct
    27781 accataagaa ggtattgtgg aagttgtccg ttaccaacat aaagcattgt accagggatg
    27841 atggtgatac cattaccagt accagcaagt ccaaggatag aagccaatcc accaccgatt
    27901 gcaccagcaa tcaatgaaag gaagaatggt ttacggaagc gcaagttcac cccgaagata
    27961 gcaggctctg taatacctag gaaggcagaa agagcagccg ggaaagcaag tgttttcagt
    28021 tttggatttt ttgttttaac accaaccgca acagtagcag caccttgagc tgtcatagca
    28081 gctgtgatga tagcgttgaa tgggttagca tggtcagcag caagtaattg cacttcaagc
    28141 aagttgaaga tgtggtgcac acctgacacg acgatcaatt ggtgaacccc accaatcaag
    28201 aaaccaccaa gaccaaatgg catgctaaga atcgcttttg tagcaataag gatgtagttt
    28261 tcaacaacgt ggaaaactgg tccaatgaca aagagtccaa ggatagacat gaccaaaagt
    28321 gtcacgaatg gtgttaccaa gaggtcaatg acatctggaa caaccttgcg gacagctttt
    28381 tcaaatttag ctccgacaac cccgatgatg aaggctggaa gaacggaacc ttgcaaacca
    28441 acaacaggga tgaaaccaaa gaagttcatc gctgttactt caccaccttg agcaactgcc
    28501 caagcgtttg gaagtgagcc agagacaagc atcataccaa gaacgatacc aacggcagga
    28561 tttccaccaa atacacggaa ggttgaccac acaaccaaac ctggcaagat gatgaaggct
    28621 gtatctgtca agatttgtgt gtaagttgca aagtcacctg gaagtggcat ttcaagagcg
    28681 ttgaaaagac cacgcacacc catgaagaga cctgtcgcta cgataactgg gatgattgga
    28741 acgaaaacat caccaaaagt acggatagca cgttggaacc agttcccttg tttagcaact
    28801 tctgctttca tgtcatcctt agatgatgtt ggtaatccaa gtacaacaac ttcatcgtac
    28861 attttgttaa ctgtacctgt accaaagata atttggtatt gccctgagtt aaagaaagca
    28921 ccttgaactt tttccaagtt ctcaatcact tctttattga ttttctcttc atctttgacc
    28981 atgacacgta gacgtgtcgc acagtgggca acactattga cattttcacg tccgcccaag
    29041 gcatcgatga ctttttttgc aatttcctga ttgttcattt gcaaaaatct ccttatataa
    29101 cattttgttc ttgtttgaaa gcgattttat tcgcccttac gactattatt ttatcatgtt
    29161 ttaaaaatat gtcaagcgtt ttgcagaaaa aatattctat ctacttagtg ggcaagcttt
    29221 agattgattg tttttgactg tttcatcagg aatttcttga aaaataagtt tgaaaccttg
    29281 gttaaaaagt cttttatttc attatctttc acattttcgt aaaaatttga ttgatttcct
    29341 gtatttattt tatgataatc gtttgacagt tttttctctt ttttaacata aagggcttgc
    29401 attttttatc gaaaacagtt actattaaaa gtgataagat ttttggagga atgaatggaa
    29461 tggacaactg agcgtcgtta cagactttat caagattgga cgcaagaaga aattcaacat
    29521 ataaaggaaa atatggcaca atctccatgg catactcatt accatgttga gccaaaaaca
    29581 ggacttctca acgacccaaa tggcttttct tactttgatg gcaagtggat cctcttttac
    29641 cagaattttc cttttggtgc agcccacggt ttaaaatctt gggcacagct agaaagtgat
    29701 gatttggttc actttagaga aactggagtc aaagttttgc cagatactcc attagatagt
    29761 cacggtgcct actctggttc tgccatgcaa tttggcgata acttattcct attttataca
    29821 ggaaatgttc gcgatgaaaa ctggatccgt cacccatacc agatcggtgc tttgatggac
    29881 aaggagggta agattacaaa gattgacaag atcttgattg accagccagc agactctact
    29941 gaccacttcc gcgatccaca aatttttaac tttaaaggtc aatactatgc tgtcgtcggt
    30001 ggacaggact tggagaaaaa aggcttcgtc cgtctctaca aggctgtcaa taacgactac
    30061 acaaactggc aagcagttgg cgaccttgac tttgctaacg accgtactgc ctacatgatg
    30121 gaatgtccta atctggtctt tgtagaggaa cagcctgtcc ttctctactg tccacaagga
    30181 ttggataaga aagttctaga ctacgataat atctttccaa atatgtataa gattggggct
    30241 tcctttgacc ctaaaaatgc caaaatggta gatgtgtctc aacttcaaaa catggattac
    30301 ggtttcgaag cctatgcaac tcaagccttc aacgctcctg atgggcgtgc tctagcagtt
    30361 agctggcttg gtttgccaga tgtttcttac ccatctgacc gttttgacca ccaaggaacc
    30421 ttctctttgg tcaaggaact cactatcaaa gacgacaagc tctaccagta tccagtcgct
    30481 gctattaagg accttcgtac ttctgaagaa gccttctcaa accgttccca aaccaagaac
    30541 acttacgaac ttgaactcaa cttggaagct aatagccaga gcgagattgt cttacttgct
    30601 gataaagaag gtaagggact ttcaatcaac tttgaccttg taaacggtca agtgacagtg
    30661 gatcgtagcc aggctggaga gcagtatgcc caagaatttg ggataactcg ttcttgccct
    30721 atcgagaatc aggctactac tgctacaatc ttcatcgata actctgtctt tgaaattttc
    30781 atcaataaag gagaaaaagt attttctggt cgtgtcttcc cacatgcgga ccaaaatggt
    30841 atcctgatca aatctggaaa cccaactgga acttactatg aattagatta tggtcgcaaa
    30901 actaactgat gtcgccaaac ttgcaggcgt cagtcctact accgtttctc gggttatcaa
    30961 taaaaaagga tatctatctg agaaaaccat ccaaaaagtc aatgaagcca tgcgagaatt
    31021 gggctataaa cccaacaacc tggctcgtag tctgcaagga aaaccagcta agttaatcgg
    31081 cttgattttc cccaatattt ccaatgtttt ctatgcagaa ttgattgata aattggaaca
    31141 ccaactcttc aaaaatggtt acaagaccat catctgcaac agtgaacatg attctgagaa
    31201 ggaacgtgaa tacatcgaaa tgttggaagc caatcaggtg gacggcatca tttctggtag
    31261 tcacaaccta ggaatcgaag actacaatcg tgtgacagcg ccgattattt cctttgaccg
    31321 aaacctatcg ccagacatcc ctgtcgtctc ctctgacaac tatgctggtg gggttcttgc
    31381 tgcccaaacc ttggtcaaga caggtgccca gtctatcatc atgattacag ggaatgacaa
    31441 ttccaattcg ccaaccggac tgcgccacgc tggttttgca tccgtactcc caaaagctcc
    31501 tattatcaat gtttccagtg acttttctcc cgtcagaaaa gaaatggaaa tcaagaatat
    31561 cttgacccgg gaaaaaccag atgccatttt tgcttcggat gatttgacag ctattctggt
    31621 cattaaaatc gctcaagaat tgggcatttc tgtcccaaaa gagctcaagg tcatcggcta
    31681 tgatgggacc tactttatcg aaaattacta ccctcaattg gctactatca agcaaccttt
    31741 ggaagagatt gcttgtctca ctattgatct tctcttgcaa aagattgaag gcaaggaagt
    31801 cgccacaact ggttacttct taccagttac gctattacca ggaaaaagta tttaaacaca
    31861 agaaaactca gaccgattcg tctgagtttt tatgatctta aattttcgag atagcgctgg
    31921 gctgtctcta ggttaaaggt tttatctgag atgaggcgct ctactagggg agcaacttca
    31981 gattcactag ccccagctag gagagctagg gatttggcct gtagtttcat gtggccttgc
    32041 tggatgcccg tacttaccaa ggctttgagg gctgcaaaat tttgagcaag accgatggac
    32101 acgataatct gggctaattc tctggcagaa ggatttccta gtagatcatg actgagagct
    32161 acacgtgggt tgaggccgat agagccaccc ttagtcgcta caggcatggg cagggtcatc
    32221 tcaccgacca attcttctct ttcaaggtcc agcgtccagc agctaagacc ttgatagcgt
    32281 ccatctcgac tggcaaaggc atgggcccca gcttcgatgg cacgccagtc attaccagtg
    32341 gcaatcaaaa tcgcatcaat accattaaaa attcctttat tatgagtagc agctcggtaa
    32401 ggatcagcct gcgcaaactg actagccaac gcaattttct ccgcaatctc tcgtccttga
    32461 tccttttggc ggctcaagta gcgaaaggcg atgcgacagc ttgcagtcac cagagaatca
    32521 gtcgcgtagt tggacaggat tcccatgaga ctctgtccct gactgagttc ttctaagact
    32581 ggtttcaagg cttccagcat ggtgttgagc atattggcac ccatggcttc ctgggtatcg
    32641 acatgaatat aaacaacgag aaagtctggt tcgcctttta tctgctcgac atgcagatca
    32701 cgcgccccac ccccacgttt aacgatagaa ggataggctt gattggcaag ctccaagagc
    32761 tccgctttct tgctggcaat cttctcttgc gctagtttag gattagcaac ttgataaagg
    32821 gctacctgcc caatcatctg gcgctgatgg acttgtgcag taaaaccacc tgcacgcttg
    32881 atgattttgc tggcatagct ggccgccgca accacagagg gttcttctgt cacataggga
    32941 acggtgtatt cctgaccgtt gacaagtacc tccggaacca gcgaataagg cagagaaaaa
    33001 gttcccacca cattctcact cagctggtct gccacagtca cactcatctg ttcatccttc
    33061 tccagactag cttgtctctc aggactaagg agcgcctgag cttttagcag ctcgaggcgc
    33121 tcttggtatg attttttaga aaatccattc caacttatct tcattatttt tcaaccttgc
    33181 tataacggcg ttggtggtca agaatttcaa ccaaggcaaa atcttgattt tcatagccag
    33241 caaactgggc agagtttgtt tcatccaagt ttacttcctc aaaaaagacc ttttcatagt
    33301 ctgcaacgga tagggcagtt cgttggttga gcttgttcaa gcggtcttta tccaaataag
    33361 cttcatatcc ttcaaccaat tcaccactga agaactcagc cacagctcca cttccgtaac
    33421 tataaagggc gattttatcc ccagctttca agctatctgt attttccaag agagacaaaa
    33481 gtccaaggaa aagtgaacct gtgtagatat tccccacctt ttgactgtag agaatagact
    33541 ggtcaaaatg cttttgtaag aggtcttttt tctcttgagg caggctctta tccatgattt
    33601 ttttcaagcc ttttagcgct aatttaggat aaggcaagtg gaaacaaaca gccgcaaaat
    33661 catccaaagt aagctggtag cgtttttgat attcaagcca agtcgttttc aaactatcca
    33721 agtattgttg ggtagaatag acaccattta cataaggagt tgtcgagtaa tttggtcgcc
    33781 agaaatccat gatgtcacgg gtctgagcta cattgtcatt attaaaggcc atcatgcgtg
    33841 gattttgtgt aatcaacata gctacacttc cagcaccttg agttggttct cccggagttt
    33901 caataccata tttggcaata tcactggcaa tgaccaagac cttggactcc ggagaatttt
    33961 ccacatgcaa tttggcataa tggagggcag ctgtcgcccc atagcaggct tctttaatct
    34021 cgaaactacg agcaaagggc tggatgccca gcaagccatg cacaaagacg gccgcagcct
    34081 tactctggtc aattcctgac tcggtagcca caatgaccat gtcaacttct tgtctttctt
    34141 gctcagttaa aatagagtca ctagcactgg ccgccaaggt cacgatgtcc tcagttaggg
    34201 gcgcaatact caattccttg agtaagagtc ctttacttaa tttttcaggg tcaattcccc
    34261 tcgcttctgc taagtcttgt aatttcaaga catattgact ggtcgcaaaa ccaatcttat
    34321 caataccgat tgtcatattt acctctgttt tatcattcat gtaaaaaatc gttctatact
    34381 attttatcac aaatggcagt aaaagagaga aaaaagactt gattcaccaa atcaagcctc
    34441 ttattggtca tcattttaaa gaatgattag ttgctagaga gttcaccgat ataagtagct
    34501 ttataagctc cattcacagt tatcagctcc tggaggatca aatttcctga gtaaatcctt
    34561 cccatctcat ctacaaattt ttgataaaac tgactgatcg gaatttctct gacatcctta
    34621 tcaaatgtct tatcaagtgt tttactaacc ttctcagcaa tcaattgatg ctcttgccat
    34681 ccactttgaa actctgagcc cgaactagaa accatgactg ggataaacaa caaggtcagt
    34741 agatttacag acaataagga aagtagtaga cttcctgcaa aactagaatc ctagttcatg
    34801 attgataatg ccagcaatca aattcattcg taatccgaag cgtttacgat gatttcgata
    34861 ggttgttgaa aacattttaa acgtttttac tttggcaaag atgttctcaa ccttgcttct
    34921 ctccttagat agcgcatggt tacaggcttt atcttcaact gttagcggct tgagtttgct
    34981 ggatttacgt ggagtttgtg cttgaggata tatcttcatg agcccttgat aaccactgtc
    35041 agccaagatt ttaccagctt atccgatatt tctgcgactc attttgaaca acttcatatc
    35101 atgacaatag ttcacagtga tatccaaaga aacaattctc ccttgacttg tgacaatcgc
    35161 ttgagccttc atagcgtgaa atttcttttt accagaatca ttcgctaatt gtttttttag
    35221 gacaattgat ttgtacttcc gtcgcatcaa tcattaccgt gtcttcagaa ctgagaggag
    35281 ttcttgaaat cgtaacacca cctttgaaca agagttactt caacccattg gctccgacgg
    35341 agtaagttgc tttcgtggat accaaaatca gccgcaattt cttcataagt gcggtattct
    35401 cgcacatata gaaagcgttg ttaatttatt tatctcattt ttcagaaaat tcttttattt
    35461 ctgtaaagtc tacgatactc gatgtgtttt tatataatga tacagtctga gaatcactgt
    35521 tccgctagcc attccaatag agattaccaa agccaacatg acaaccaagg tcgcacttgc
    35581 cagtgcttta ttatagtccc ctgtcacaaa aaaggcagtt gttcggtagg agagataacc
    35641 tggaaccagc ggtgccaaaa tggccaagat aaagaccaca gcaggtgtct tataaagaat
    35701 acttaaaatc tggctgacac aagaaccaat aatggctgca atgaaggtag ctacaatgac
    35761 attggtcggt tccttgagca agagatagat tagccagaca gtcatgccca aaatccctcc
    35821 aggtaagagc atagaccgtt gcacattgag tacgattaaa aaagtgataa tggcaagaaa
    35881 acttgctact gcttgtaata aaaaggttgt tagtgtcata ttagttcatc aataccaagg
    35941 cgacagaagt tcctgcccct aaagcgaggg taatgagcag ggattcaaac atcttactca
    36001 taccagagtt tatgtggttg gtcataatat cacggaccgc attggtcaag gcaatacctg
    36061 gtacaaacgg catgaccgca ccagctataa tcaaatctgc cgttgaagga aaacctgtgt
    36121 agcgagccca aaactgggca attatcccaa agacaaaggc tccagcaaag gctgtcacaa
    36181 agggaattcg gataaatttt tccacataga gggaaaaggc aaaaccaaat aaggtcgcca
    36241 ctcctgcccc aagtgcgtcg tagatatttc cgctaaacat aactgaaaag aaaggagcac
    36301 taaaggtcgc agccagagtt acctgcaact tagtataggg aaggggtttg gcttgcaagg
    36361 ccgtcaattg cttaaaggct gtttctaagt caatctgccc cccaactagc tgacgagaaa
    36421 tctggttcac atcgcagact ttttcgatgt tataagaaga ggaggtcacg cgcttcatgc
    36481 gcgaaatatt ggtattttca atagagaaaa agatagcggc aggcatggca aggacattgc
    36541 aatccacaat cccctgcgaa tgcgcgattc gaatcatggt atcttctaca cgatggattt
    36601 ctgagccact tttaaggaga atagtccccg ctagcataat cacatcaatg acggcattta
    36661 attcttttga ttcttccatg ctttcctcct tttatcaact ccctctattc tatcacaaat
    36721 ccggactcaa aaaaaatctt tgccatgaaa tcatgacaaa gattgattac tcattttgat
    36781 tatccatctg cttttaagga gtagctgaag ttgttttagg tttgtagatt gaaatcttga
    36841 ctctagtctt atttaggtct accttttcac ctgctctagg actttgttca acaaccatgc
    36901 cttctacact acctgcaggc gctgtcgtca cttctacaac ttctatatta gcttccttaa
    36961 tcccaacaat ttgaatcaaa ttgttcttag taaactccaa gctggaacca atgtaactcg
    37021 gcatggcaac acttgtaact tttttagcta ctgtcaagac aatttgagta ggtttactca
    37081 catcataagt cgttccggca cctggacttt gtttcataat cgttcctggt tcgctttcgc
    37141 tggactcttc ttcctctatc ttaatcaaat tctcaggaac cttcttctgc ttgagttctg
    37201 agattacttc tgtagagttc cgtccaatat agttccctaa ttgaatcgtc gtagcttttt
    37261 tagctactgt caaaacaatt tgagttgcct tgctcaagtc ataggtcgta ccttctggta
    37321 gactttgctt caagaccgtt ccagcctcac tctcattgga ctcttcttcc tcaattttaa
    37381 tcaaattatc tggaactttt ttctctttta attccgcgat aacatcagaa gatttccggc
    37441 cgacataatt actaatttgg aaggattgtt tgcctgatga gacaaccaga ttgattttcg
    37501 ttccttcttt tcgaccagtt ccagcgccag gatctgtacg gataatccgc ccttcttcca
    37561 ccttttcact agcctctgtc ttctcctcac caatctcaaa attggctttt ttgagcgttg
    37621 ccttggcctc tgcaactgtc tgacctgcca catccggaat ggcaatggtt gcaggagttc
    37681 tggataagat ccaaataaga gaagctgcca ccaatacaag gctggccaac aaaatcaggt
    37741 aacgcatctt aaatctatgt tttttcggtg cttgtggttg gtaagtttcc tctgtcacag
    37801 cctggcttgg gtttttgatt gatttgtgtt ctgtctgcgc ttgaacctta ggaatagatg
    37861 tcaaggtact ctgagaaacc ttcggcaagg tcttggtatc tgccttgctc gtttcatcaa
    37921 agattaactt actttcattt ctacgattgt aggacaagct actagacaag tccacataca
    37981 tctctgaaac cgatcggtag cgattggtca actttttagc agttgccttg ataataacat
    38041 tttctaaagc ctgaggtaca gatggatttt ctgcaataac ggacggcagg ggtttctgga
    38101 aatgctggag ggcaatggtc accgcgctat ccccgtcata agggatatgg cctgtcaaca
    38161 tctcatagaa aataatcccc atggcataga tatcactctg cacagtcgcc ttagaaccac
    38221 gcgcctgctc tggtgacaag taatgaactg agcccaacat cgagttagtc tgggtcagac
    38281 ttgtctctgc aaaggctaca gcaatcccaa agtctgtgac cttggcagtc ccatctggtg
    38341 tcaaaaggat attttgaggt ttcaagtccc tgtgaacaat tcctcgagta tgggccaagc
    38401 gcatagccaa gagaatttgt cccatgatac ggacggcttc ttcattagaa agaggataat
    38461 gttccttgat atagcgtttg aggtctagtc cagcaacata ctccattgca agatactgtt
    38521 gaccgtcttc ctcaccaata tctgttatcc gaacgatatg aggatggtct agatctgcca
    38581 tagctctcgc ttcacgctga aaacgagcta cagctatcgg gtccgtctgg tagttggtcc
    38641 tcagaacctt cactgccact tcttccccat ctaagattaa gtctttggct aggtaaacat
    38701 ccgccatgcc tcctcgacca atctgtttga caatccgata gcgtccggca aaaatcttgc
    38761 cgatttggat cattcttcat cctcctcgtt catagaaaca agggcaaccg taatgttgtc
    38821 taaacctcct gcattgttag caaaacgaac aagtgtctcc gttttatctg ctaaaggaat
    38881 atcactggtt acaatatcac gaatctcact gcctgaaatc atgttggtca agccgtcact
    38941 attgagcaag agatagtcac ctgactcaag gataactgtc ccaaaatcag gctgaatttc
    39001 atctttttgc ccaatagact gggtgataat atttttttgc ggatgagctt ctgcctcttc
    39061 tggtgtcaat tgaccagcct tgagcaattc attaaccaag gaatgatcgc tcgtcaactg
    39121 atggtattct tctccacgaa tcaagccgat acgcgaatca ccaatatgag catagatagc
    39181 ctgattatca ataatagcaa ggacttccaa agtagttccc atgcctctgt aagcttcatc
    39241 ctgaccaagc tggtgaatct tttgattttc aatttctagg taatgggcga accattcacg
    39301 cacttcattg actgtatcga tctgggtatc aacccaagct acacccaggt ctgtgaccgc
    39361 catttcacta gcgatattcc ctgcacgatg acctcccatc ccatcagcta aaataatcat
    39421 ggtacgtcca gctctattga catagtggtt gacatagtct tggttatttg ttcgtttctg
    39481 accaacatct gttaataatg aaatttccat gtgtcagttc cttcctaatc cgatatcttg
    39541 cgaaattgac tgatgaagaa tccatcactt ccatacaatt caggtgtaat gaggatacag
    39601 ccgtctttca tgatatcctt acattcatgt tctagtttta cctgctcgaa ctcgggatga
    39661 ctctctaaaa aggccttaac gacttgaaaa ttctcctctg agacgatagt gcaggtgcta
    39721 taagttatta taccaccttt gcctagtatt tgacaaacac tacctaatat ttctaactga
    39781 atttcctgca aggacgcgaa atctgccgtt tctttattgt atttgatatc tggttttcgg
    39841 cgcaaaagac cgattcctga acaaggagca tccaccaaaa tcttatcaaa ggaatcctgg
    39901 tcaaaaaact catgcacctt tctggcatcc aatttttgag tttgaacccg atcttcaact
    39961 cccagacgtt gggcattttc ttgaattaaa tccaacttgt ggtcgtacaa gtccagagca
    40021 gtaacctgac ctgtcgtaag ataagaggct atatgggctg ttttcccacc tggagccgca
    40081 caggcatcaa gcactcgctc atcaccttgt aaatcaagcg tcggagcaac cagctgactg
    40141 gactcgtctt ggatggtaat ggctccatcc gcaaacaaat tatgccctgc aaaatgcccc
    40201 tgctccttaa ccagaccagt ggttgctaaa agggaatcat tcgcctccaa caaggcttgg
    40261 atttcctctt ttcgacttag gtctgttaca cgaatactgg ctttgtttcg cactaacagg
    40321 ctttcaaaga tggcttttgc tctctcctct ccgtattctt ccttgagttt ggcaactagc
    40381 caaactggga gagaataggc aatggagtca cgcttgtttt ttcgcttgat gctagcaata
    40441 tctggccagc cttcacgcaa gatacggcga aggagggcat tgaccaattt ttcactgcct
    40501 tttttacgaa gtttggccaa ttccactgct tcattgacca cagcatgatc tggaatcttg
    40561 tccaaatagc ggagttggta ggcactcatg agaagaagga catagagcca gctgtctaac
    40621 tggtctctgt cttcgataaa gtgggatagg taccattcca gagtcagttt acgggctacc
    40681 gttccataga ccagctcggt cactaagccc ttgtctgctg ccaaaagttg actccccttt
    40741 agatgcttat ttaaggcgat atttgagtat gcttggttca caaaaacatc ctctagcact
    40801 gctagagcta aacttctagc cgtttctact ttagtcacca aatcgttctc ctacagtcaa
    40861 tgtacgtcca actccgttga ggaaggaagc aatgtccatc ttaggcttac cagctggctg
    40921 cacttgtttg agggatagag ccccttcagc cgttgcgaca atcaattctt tcttgccgat
    40981 agagagaatc tcacctggat ttccctgacc ttctactggt agggcttcat aaatcttaaa
    41041 gcggtcgccc ttaaggaaag catgggcaac aggccagggg ttcattccac gaatttggtt
    41101 aaagagttga cgattggttt tgttccagtc cagtttttct tcctctggct ttatatttgg
    41161 agagaaggta acctgactcg tatcctgcgg ttcaggtttg atatcaccag caatataggc
    41221 aggcagagtg tccaaaagca aatcacgacc aactagcgcc aatttttcaa acaaggtgcc
    41281 aacattgtcc tcatctgtga tcggaatgct gcgacgagaa atcatatctc ctgcatccat
    41341 ttccttaacc atttccatga tggtcacacc agcttcctca tccccttgaa tcaaggcata
    41401 atggataggc gcaccaccac ggtgtctagg aaggagggag gcatgaacgt tgacagcaaa
    41461 gtccatgcta tcaaggagtt tgcttgggag aaactgccca aaagcagcag tcacaattcc
    41521 atctgctcct agcttcataa gttcttccat ctctggactt ccagataatt tttcaggttg
    41581 gtagatagat agtcctgctt ccttggcagc ctgcttgact ggggtttctt ggataacttt
    41641 tttacgacca acagcacggt ctggctgggt cacaacggct agaatttcgt aacggtcatc
    41701 tgtcaaaagt ccttttaaga ctgttgctga aaagtcgggg gtccccataa agattagttt
    41761 tgtcatatct tctccttctt ataaaaattg ctgcggctca tggtcaatgc tgagacggag
    41821 ctcactattt tcccgttctt gagtcaaggc caagacctgg ttgagggtcg gccccagctc
    41881 atcttctaaa cggtatttaa ttaaaatctg gtaatgatag aggttgtggg tacgggcaat
    41941 cggttttggc gttggcccca gaatgggact ggtctctgac aagcctgacc gcaaaatgtt
    42001 catgacttca taggcacgtt tgaaaacctc ttcttctttc ttgtgagaaa gggtaatacc
    42061 aatcgtgaaa tagtaaggtg gatagccgag ttgtcgtctg attcccattt cataggcata
    42121 aaagccttcg taatcttgat ccttggcaaa tcgaatagca tagtgctgcg aattgtagga
    42181 ctgtatcaag acttgacctg ctttttcagc ccgacctgct cgacctgcca cctgagtcaa
    42241 gagctggaag gttctctcag aagaacggaa atcaggcaga ttcaaggccg tatccgcatt
    42301 tagaactccg actagggtaa cattgggaaa atccaaaccc tttgcaatca tctgagtacc
    42361 aagtaaaata tccgcttccc ctcgcccaaa ctggtcaagc aaggcttggt gactgccttt
    42421 ctttcgagtc gtatccacat ccatcctcaa aatgcgagct tggggaaaga gttctgctag
    42481 ctcatcataa gccttctgag ttcccgtccc atagtaacga atactgcggc tcttacagtt
    42541 aggacagacc tgaggaatat ccttcgagaa accacaataa tggcagttca tagtcttggt
    42601 atccatatgc aaggtcagag aaatatcgca gttgggacaa gtatccaccg tcccacactc
    42661 ccgacacatg acaaagctag aataaccacg gcgattgagc atgagaacca cctgctcttt
    42721 tttaaccaga cggtcttgga tagcctctag caaaggaggc gtaaagtttg acgtctcatt
    42781 ttgtccgata tagtctcgaa agtcaatcac ttgaacctca gggattgtag ccaaaggatt
    42841 agcacgttgg gttagacgta agtgttgata gacgcctttg ccagcacgtg cccggctctc
    42901 taagctcggc gttgcagatc caagtaccag agttgcttga ttatactgag cccgtaaaat
    42961 agctacatct ctggcatggt aacggggatt gctgtcctgc ttataagcca cttcatgctc
    43021 ttcatcaata atcatgacac ccagattttt cagaggagca aagatagcag atctggcacc
    43081 aacaacaact tgggcatcgc cacgctccac cttgcgccat tcatcatact tttcaccatt
    43141 ggataatcct gagtgaagaa tggctacctt gtccccaaaa cgtgctataa aacgctcggt
    43201 catctgagga gtcaaggaaa tctcaggtac cagcaaaata gctgtcttgc ccttatccag
    43261 ggcaccttgg ataatctgca agtaaacctc ggtcttccca cttcctgtaa tcccttgaag
    43321 tagaaaggga ggttgagaac tgccaataga actcacaacc gcatcacgcg cctgtctttg
    43381 ttctggattt aactccaaag gtctacttgc ttcaattcct tcaaaataag cagccgagcg
    43441 ttgaacttcc ttttggacta tggtaacagc accttgatcc acaaagaagt tgacttgctc
    43501 tcgcgagtag gactctaaca agctagccaa ggaagcgctc tctggatgag acagcagata
    43561 atctctcagt tccaactttt tcttggcacg tgtagaaatc tcaacacctt ctaattgagc
    43621 atggtcaacc tcataccaag actgggtctt gaccttcttt tgatcgactg cctgatattc
    43681 cagaccaagc aggccttttc tagtcaaacg catcatttca gcttgcttgg caaggtctag
    43741 tgaagaaaag gctagcgaat cttctgaacc aaacaggcgc actcgttctt cctgactcaa
    43801 gccttccaga ggatagagaa tcttgtcata gctagaattc agaaatcctg gaagcatggc
    43861 cttgaggata gaaattttgt aggagaagac agatttgcgt aactcctcag ccagccagag
    43921 ttgttctggc gtgagaacag gagaaaaatc cagcacctct gcaatatctt ttaaatcttg
    43981 ctccatctct tctccatctg attgggactc caaaccaaga acaatccctt gaatcaggcg
    44041 attcccctta ccaaaaggca catgaacccg catcccaact tccagcattc cctcaaattc
    44101 ctccggaatc ctgtaactat agggctggtc cgtctgcatc aagggcacat ctacgataat
    44161 cttagctagg gccatcgtct cacctcctcc ttgtcagtac attcttgcaa tagaaaaaat
    44221 aagattgagt ccccccaacc ttaaattttt tcaccatctt ctttttcttt agcaatttgc
    44281 tctttgattt tcttttcttc ttcttctttg cggcgttttt cttcttcgat acggcgacgc
    44341 actgcttcac gttttccttc tggatctggg tgaattgtaa cgtttcctga ttcgatttct
    44401 tctaaagcgc gaagagttga tttttcagac ttaaaacctt gagttgctgg ggcacctgct
    44461 tccaattcgt gggcacgttt tgcttccaag attacgagtg aatattttga aggaaccttg
    44521 tcgagcaagg tatcaataga gggttttaac atcatttgct tgtacctatt ttctaaattt
    44581 tatcgggtag ttggagattt tggtaacatc tcctgatagt gaccaatgac acgatccaca
    44641 cagaagtgtt ctgcttcaat cacacatttg acacgttcag cagctagggg tacctgatcg
    44701 ttgacaatcg cataatcata ctcacgcatg agggcaattt cttccttggc cttttcgatt
    44761 cgttgggcaa tcacttctgc actatctgtt ccacgaccta ccaagcgatc ttgcaattca
    44821 tccaaatctg gtggtgtcag gaagataaag acagcatctg gaaccttttt cttgacctga
    44881 agagcaccct gaacttcaat ttcaaggaaa acatcgattc ccttgtccaa ggtttcattg
    44941 acataggtca gaggagttcc atagtagttg ccgacatatt ctgcgtattc caacatctgt
    45001 ccttgacgaa tcagctcttc aaattcttca cgagtacgga agaaatagtc aacaccgtcc
    45061 acttctccag gacgttgtgc gcgtgtcgtc atcgatacag aatattgaaa ttggttttca
    45121 gaactctcaa aaatctctct tctaaccgtt ccttttccaa cccctgaagg accagaaaaa
    45181 acgattagta agcctcggtc tgccattgtg tctcctttta gtcaatctgt gaaataacat
    45241 ttctctagaa taatggcaaa aagccagatt atcctttaca gtctttctat ctagtgtaac
    45301 aaaaaagcag taatttttca actgctcttt cttatttatt tagcataatc tactgcacga
    45361 agctcgcgaa tcacggttac cttgatattt cctggataat cgagattgtt ttcaattttc
    45421 ttacgaactt tgtgagccaa gattgtgact ttgtcgtcct tgatttttcc tggattgacc
    45481 atgatacgaa tttcacgtcc tgcttgaagg gcaaagctag tttgcactcc ttcaaagccg
    45541 ttagcaattt cttccaaatc atggagacgc ttgatgtagc tttcaagaga ctcactacga
    45601 gcacctggac gggctgcgct caaggcatct gctgcagcga cgataactgc tatcacgctc
    45661 tcagcttcaa catctccgtg gtgactagca atcgtattca ccacaactgg gggttccttg
    45721 tacttacggg ccaattccat accgatttca acgtggctac cttcaacctc atggtcaatg
    45781 gctttcccga tatcgtgaag gaatccagca cgacgggcaa gagccgcatt ttcaccaagt
    45841 tcgctcgcca tgataccagc caacttagca acctcaatcg aatggcgcaa aacattttgt
    45901 ccatatgaag tacggaactg caaacgtccc ataatcttca tcaagtctgg atgaaggttt
    45961 ggcgcaccaa tttcataggc agcagcctca ccgtattcac gaatcttatt gtcaatctct
    46021 tgacggtttt tctcaaccaa ctcttcgata cgagctggat gtatacgacc atctttgagc
    46081 aacatttcca tagtcatacg ggcaatctca cgacgaatcg gatcaaatcc tgacaaggtc
    46141 accacttctg gtgtatcgtc gataatcaca tcgacccctg tcaaactttc aaaggtacga
    46201 atgttacgac cttcacgacc aataatgcgt cccttcatag tatcgtctgg cagatgaact
    46261 gttgagtttg ttgactccgc tacatattca ccagcgatac gttgcatagc ttgaaccaag
    46321 atgtccttgg ccattttgtc agaacgttcc ttgacctctt gctcagcttc gcgaatgcga
    46381 ctggcaatct ccctggtcaa gttttcctct gtctgagcca agataatatc tcgtgcttct
    46441 gcctgagaca gagcaccaat acgctctagt tctgcttctt tttgtctttc gacttcctct
    46501 aattgctctt cacgcgcatc aaggtttttc gctctatcag aaatactttg ttctttttgt
    46561 tcaagtgttt gttctttact cgtcaaattg tcgtccttac ggtcaaggct agtagctctc
    46621 tctgtcaaac gactttcgat ttgtttgagt tcttgacgtt ctgatttgaa ttcagcgtcc
    46681 acttcttcac ggtattttct ggcttcttct ttggcctcca atagtgcttc ttttttaaga
    46741 gacttgcttt cacgtttggc ttcattaaca agtaaatccg cttcacgctc agcttgtcca
    46801 cgtaaattag ttgcttcttg ttcagcattt aaaagcatca actctgcagc ttcctgagat
    46861 gatttcatct tagctgagat gctgacatat ccaatgacta aaccaatgat gacggcaaaa
    46921 acagcaatcg caagcgacat gatttccatg tttttacctc attttattgt tattccgaat
    46981 gacatacatt cttttacatt ctaccataaa aaaagaattt aaacaaacct taaatggata
    47041 tcgctaacat ccgcattatc actatcatag ttatcattta cagatataaa gcgaatacct
    47101 ttttcaggaa atatcttttg taagtacttt cctgactcga tataatctct gccgaaacga
    47161 gataaatctt ttaccataat gatagaaaac tttcctttat tcaggtcatc aatcatattc
    47221 ttaaaatttg gtcgctcaaa attagaacca ctatatccat catctacata ttcataggat
    47281 aattcaatag atttctcttc tacctccaaa aagcaaaaag gataaatttc ccctacactt
    47341 taatagtgaa aatttatccc tcttggtaag ctcttttatt aatttttttg ataaag
//
LOCUS       DAVLVF010000006        45227 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVLVF010000006 DAVLVF010000000
VERSION     DAVLVF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45227
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.006"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            86..1777
                     /locus_tag="VYN66_000309"
     CDS             86..1777
                     /locus_tag="VYN66_000309"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV1494395.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            complement(2092..2619)
                     /locus_tag="VYN66_000310"
                     /pseudo
     CDS             complement(2092..2619)
                     /locus_tag="VYN66_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2626..3102
                     /locus_tag="VYN66_000311"
     CDS             2626..3102
                     /locus_tag="VYN66_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1494396.1"
                     /translation="MDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYTSI
                     YFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            3186..3893
                     /locus_tag="VYN66_000312"
     CDS             3186..3893
                     /locus_tag="VYN66_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1494397.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            3894..5225
                     /locus_tag="VYN66_000313"
     CDS             3894..5225
                     /locus_tag="VYN66_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV1494398.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            5392..6267
                     /locus_tag="VYN66_000314"
     CDS             5392..6267
                     /locus_tag="VYN66_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV1494399.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            6385..7248
                     /gene="pstC"
                     /locus_tag="VYN66_000315"
     CDS             6385..7248
                     /gene="pstC"
                     /locus_tag="VYN66_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1494400.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            7241..8056
                     /gene="pstA"
                     /locus_tag="VYN66_000316"
     CDS             7241..8056
                     /gene="pstA"
                     /locus_tag="VYN66_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1494401.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            8058..8810
                     /gene="pstB"
                     /locus_tag="VYN66_000317"
     CDS             8058..8810
                     /gene="pstB"
                     /locus_tag="VYN66_000317"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1494402.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            8825..9475
                     /gene="phoU"
                     /locus_tag="VYN66_000318"
     CDS             8825..9475
                     /gene="phoU"
                     /locus_tag="VYN66_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1494403.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            9543..9959
                     /locus_tag="VYN66_000319"
     CDS             9543..9959
                     /locus_tag="VYN66_000319"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1494404.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTA"
     gene            complement(10082..10522)
                     /locus_tag="VYN66_000320"
     CDS             complement(10082..10522)
                     /locus_tag="VYN66_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1494405.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLEVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVFDK
                     IFEEYYDRLPEEERFIIPNYSYLALANYTVQKLPEKLVEILSFW"
     gene            10734..11750
                     /locus_tag="VYN66_000321"
     CDS             10734..11750
                     /locus_tag="VYN66_000321"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1494406.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            11772..12671
                     /gene="galU"
                     /locus_tag="VYN66_000322"
     CDS             11772..12671
                     /gene="galU"
                     /locus_tag="VYN66_000322"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV1494407.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(12743..13420)
                     /locus_tag="VYN66_000323"
     CDS             complement(12743..13420)
                     /locus_tag="VYN66_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1494408.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(13404..13943)
                     /locus_tag="VYN66_000324"
     CDS             complement(13404..13943)
                     /locus_tag="VYN66_000324"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV1494409.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            complement(13955..15085)
                     /locus_tag="VYN66_000325"
     CDS             complement(13955..15085)
                     /locus_tag="VYN66_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV1494410.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDDAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            complement(15153..15851)
                     /gene="dapD"
                     /locus_tag="VYN66_000326"
     CDS             complement(15153..15851)
                     /gene="dapD"
                     /locus_tag="VYN66_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV1494411.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            complement(16081..16992)
                     /locus_tag="VYN66_000327"
     CDS             complement(16081..16992)
                     /locus_tag="VYN66_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1494412.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            complement(17057..19522)
                     /locus_tag="VYN66_000328"
     CDS             complement(17057..19522)
                     /locus_tag="VYN66_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1494413.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            19664..20920
                     /locus_tag="VYN66_000329"
     CDS             19664..20920
                     /locus_tag="VYN66_000329"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV1494414.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            complement(20998..23061)
                     /locus_tag="VYN66_000330"
     CDS             complement(20998..23061)
                     /locus_tag="VYN66_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1494415.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLVRTKELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(23063..23341)
                     /locus_tag="VYN66_000331"
     CDS             complement(23063..23341)
                     /locus_tag="VYN66_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494416.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            23493..24341
                     /locus_tag="VYN66_000332"
     CDS             23493..24341
                     /locus_tag="VYN66_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1494417.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            24963..25535
                     /locus_tag="VYN66_000333"
     CDS             24963..25535
                     /locus_tag="VYN66_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494418.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(26579..28837)
                     /locus_tag="VYN66_000334"
     CDS             complement(26579..28837)
                     /locus_tag="VYN66_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEV1494419.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(28863..30380)
                     /gene="malQ"
                     /locus_tag="VYN66_000335"
     CDS             complement(28863..30380)
                     /gene="malQ"
                     /locus_tag="VYN66_000335"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV1494420.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            30930..32201
                     /locus_tag="VYN66_000336"
     CDS             30930..32201
                     /locus_tag="VYN66_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1494421.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            32312..33604
                     /locus_tag="VYN66_000337"
     CDS             32312..33604
                     /locus_tag="VYN66_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1494422.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            33606..34448
                     /locus_tag="VYN66_000338"
     CDS             33606..34448
                     /locus_tag="VYN66_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1494423.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            34701..35501
                     /locus_tag="VYN66_000339"
     CDS             34701..35501
                     /locus_tag="VYN66_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV1494424.1"
                     /translation="MLPYPFSFFSSILGFRKPLSKRFGLNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTVSQASSVVIG
                     PSQIKELPKNLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            35511..36497
                     /locus_tag="VYN66_000340"
     CDS             35511..36497
                     /locus_tag="VYN66_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1494425.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            36633..36713
                     /locus_tag="VYN66_000341"
                     /pseudo
     CDS             36633..36713
                     /locus_tag="VYN66_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(36878..37819)
                     /locus_tag="VYN66_000342"
     CDS             complement(36878..37819)
                     /locus_tag="VYN66_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1494426.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(37797..39560)
                     /gene="aspS"
                     /locus_tag="VYN66_000343"
     CDS             complement(37797..39560)
                     /gene="aspS"
                     /locus_tag="VYN66_000343"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV1494427.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            complement(39841..39921)
                     /locus_tag="VYN66_000344"
                     /pseudo
     CDS             complement(39841..39921)
                     /locus_tag="VYN66_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(39957..40187)
                     /locus_tag="VYN66_000345"
     CDS             complement(39957..40187)
                     /locus_tag="VYN66_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494428.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(40205..40846)
                     /locus_tag="VYN66_000346"
     CDS             complement(40205..40846)
                     /locus_tag="VYN66_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1494429.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVAFAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(40858..41580)
                     /locus_tag="VYN66_000347"
     CDS             complement(40858..41580)
                     /locus_tag="VYN66_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV1494430.1"
                     /translation="MKKKRRLLFLMSIVLGMFVGMFKARVESHEIILDVKALMPWISA
                     ICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITG
                     VAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFAT
                     PKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQLLAFL
                     LVGAIHVYINVMQLPMVKRYFK"
     gene            complement(41583..41780)
                     /locus_tag="VYN66_000348"
     CDS             complement(41583..41780)
                     /locus_tag="VYN66_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494431.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNLYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(41780..41986)
                     /locus_tag="VYN66_000349"
     CDS             complement(41780..41986)
                     /locus_tag="VYN66_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1494432.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(41996..42310)
                     /locus_tag="VYN66_000350"
     CDS             complement(41996..42310)
                     /locus_tag="VYN66_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494433.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     FQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(42565..43854)
                     /locus_tag="VYN66_000351"
     CDS             complement(42565..43854)
                     /locus_tag="VYN66_000351"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV1494434.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETISMAAYFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(43932..45179)
                     /locus_tag="VYN66_000352"
     CDS             complement(43932..45179)
                     /locus_tag="VYN66_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV1494435.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDVISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
BASE COUNT        13779 a         9713 c         8575 g        13160 t
ORIGIN      
        1 caaaaaaaca ggagatttca aaaatttttt cttctttctt taaaattgtg ataaaatagt
       61 agaaaagtcc aaaaaggagc tcttaatgaa tacaaaagaa ttgattgcta gcgaattatc
      121 tagcatcatt gatagcctgg accaagaggc tattttaaag ttactggaaa cccctaaaaa
      181 ctcagaaatg ggagacattg ctttccctgc tttttctctt gccaaagtcg aacgtaaagc
      241 accacaaatg attgcggctg aactggctga aaaaatgaac agccaagcct ttgaaaaagt
      301 tgtcgcaaca ggaccttacg ttaacttttt ccttgataaa tctgccattt ctgctcaagt
      361 attgcaagct gttaccactg aaaaagaaca ctatgctgac caaaatattg gcaaacaaga
      421 aaatgttgtt atcgacatgt ctagtccgaa tatcgctaaa ccattttcta ttggccacct
      481 gcgttcaact gttatcggag atagcttgtc acatattttc caaaaaatcg gttatcaaac
      541 ggtcaaggtc aaccatttgg gagactgggg taaacaattt gggatgttga ttgttgccta
      601 caaaaaatgg ggcgacgaag aagctgtaaa agctcatcca atcgatgaac tccttaaact
      661 ctatgtccgc atcaacgctg aagctgaaaa tgaccctagc ttggatgaag aagcgcgcga
      721 atggttccgt aaacttgaaa atggagatga ggaagctctc gctctttggc aatggttccg
      781 cgatgaaagt ttagtggaat ttaaccgcct ttacaatgaa ttgaaggttg aatttgacag
      841 ctataacgga gaagccttct acaatgataa gatggatgca gttgtagaca ttctttctga
      901 aaaaggacta cttcttgaat cagaaggtgc ccaagttgtg aatcttgaga aatatggaat
      961 tgaacatcca gccctcatca agaagtctga tggtgcaact ctctatatca cacgtgactt
     1021 ggctgcagcc ctctaccgta aaaatgaata ccaatttgct aaatctatct acgtcgttgg
     1081 tcaagagcaa tctgcccact ttaaacaact caaagctgtc ttgcaagaga tgggctacga
     1141 ttggagtgac gacattaccc acgttccttt tggtctggtt acaaaagaag ggaagaagct
     1201 ctctactcgt aaagggaatg tcatcttgct agagcctact gttgcagagg ctgttagccg
     1261 tgccaaggtc caaatcgagg ctaaaaatcc tgaactagaa aacaaagacc aagtagcaca
     1321 tgctgttggg gttggagcca ttaaattcta tgacctcaaa actgaccgta caaatggata
     1381 cgacttcgac ctagaagcta tggtatcctt cgagggtgaa actggacctt acgttcaata
     1441 tgcctacgct cgtatccaat ctatcttacg caaagccgat ttcaaaccag aaacagctgg
     1501 caactatagc ttgaatgata ctgaaagctg ggaaatcatt aaactcattc aagacttccc
     1561 acgtattatc aaccgtgcgg cagataactt tgaaccttct atcattgcta aatttgcaat
     1621 tagcctggct caatccttta acaaatacta tgcacataca cgtatcttgg atgaaagccc
     1681 agaacgcgac agccgtctag ccctcagcta cgcaaccgca gtcgttctca aagaagccct
     1741 tcgcttgctt ggagtagaag cgccagagaa gatgtaaatc gccaaagtta cttgattgca
     1801 aagcaatcta agtaactaac gccaaatcct attcggactt tggctaggcg cttcactagt
     1861 tttaggacat aaatatgatc gatttatgtc ctaaaacgtc gtaatcttaa agttattgga
     1921 actaaagttc ctaattgtta gacgctagcc gcttatatgc gaactagcta actgcttcac
     1981 tagtcatgac ccctaaatat aatcgattta gaggccatga cgtcgtaacc agagacactt
     2041 gcctaaacac ttcactaatt cacctaacca aataatagac ttcctgcaaa actagaatcc
     2101 tagttcatga ttgataatac cagcaatcaa attcattcgt aatccgaagc gtttacgatg
     2161 atttcgatag gttgttgaaa atattttaaa cgtttttact ttggcaaaga tgttctcaac
     2221 cttgcttctt tccttagata gcgcatggtt ataggcttta tcttcagctg ttagcggctt
     2281 gagtttgctg gatttacgtg gagtttgtgc ttgaggatat atcttcatga gcccttgata
     2341 atcactgtca gccaagattt taccagcttg tccgatattt ctgcaactca ttttgaacaa
     2401 cttcatatca tggcaataga aacaattctc ccttgacttg tgacaatcgc ttgagccttc
     2461 atagcgtgaa atttcttttt accagaatca ttcgctaatt ctttttttag ggcgattgat
     2521 ttgtacttcc gtcgcatcaa tcattaccgt gtcctcagaa ctgagaggag ttcttgaaat
     2581 cgtaaaacca ctttgaacaa gagttacttc aacccattgg ccccgatgga taaagaagac
     2641 ttgataagaa ctctagagtt agctccccat gttgaaggtg gctattttag acaaacaaaa
     2701 aaagcttcag acactataaa ccttgctggt aagggaaggg cgctttatac cagtatttat
     2761 ttcttattag aagaaacgaa tccttcacat tttcaccgat tgacagcaga tgaaatctgg
     2821 tattttcatg caggatcccc acttactgtt cacatgatta cagcagatgg tcactacgaa
     2881 gcagtcactt taggcctgga catatccaaa gggcaacaac ttcactactg tgttcctaag
     2941 ggcactattt ggggatcaac agttgataag gattatgctc tggtttcttg tcttgttgct
     3001 cctggttttg aatttgagga ttttgaatta tttgaaaggg tagatctact ggcgacctat
     3061 ccagagcaca aggagatgat tgagcgtttg accagatact agtcttgata agcaaattta
     3121 atctgattta gcaatgttag gatttctgat aattgtgtta taatgttacc aataggagat
     3181 aatagatgac aaaacaagtc ttattagtgg atgatgaaga acacattctg aaattgcttg
     3241 actaccattt aagtaaggaa ggcttttcta ctcaattggt aacaaatgga cggaaggcct
     3301 tagctttggc agaaacagaa ccctttgatt ttatcttgct tgatatcatg ttaccacaat
     3361 tagatggcat ggaagtttgt aagcggctga gagccaaagg cgtcaaaact ccaattatga
     3421 tggtttctgc gaaaagtgat gaatttgata aggttttggc cttggaatta ggggctgatg
     3481 actacctgac caagcctttt agccctagag aattgctggc gcgtgtcaag gctgtcctca
     3541 ggcgaactaa aggagaacaa gaaggagatg attcagataa tatcgctgac gattcttggc
     3601 tatttgggac cttgaaagta taccctgagc gtcatgaagt ctacaaggcg aataagttac
     3661 tgagtttgac cccaaaagaa tttgaactct tgctctatct tatgaaacat cccaacatga
     3721 cactgactag agagcgtctt ttggaacgta tctgggggta tgactttggg caggaaacac
     3781 gtttggtgga cgttcatatt ggtaagttga gggaaaaaat tgaagacaat cctaaagccc
     3841 ctcaatttat tcgaaccatt cggggttatg gttataagtt caaggagtta tagatgaaac
     3901 gctaccttca attttggcta gtcaatctaa gtgttagcct cattctgatt gcagggatgg
     3961 cattaacttg gatcagtaaa ggcatcggtc tctttcttct agccctttct ttgggacaag
     4021 gtggctactg gctcttttgc ctttggaaat gggaagttgc ttttgagacc ttgcaccaac
     4081 ctctcttgac cagtagtgaa tactttttag aaaaagggca agaagactta aagtctttgg
     4141 ctcagtatgt gtctggccta aaaaccaaag tctcccaaca ggatcagcaa tacaaggacc
     4201 tagccgaaac aatggaggtt cttctgtcgc acctaaccat ggggacgttt ttagtttccg
     4261 ctcaaggtca gatgttatta tccagtcgct ctctgcctca ttattttccc gatgtggacg
     4321 gcgacataag ttcacttgat gacctcaaac ggatggatat tcggaattta gttcaccaag
     4381 cctttgatca aaaaacaagg ttaaaacaag aagtaagtgg gtttcatgag ggtgacttga
     4441 ttttagaagt gacagcagtt cccgttttta gccccaccca atctgtggaa gctgtgctcg
     4501 tcttgctata tgatttaaca acgattagaa cttatgaaaa gttaaattta gcctttgtct
     4561 caaatgcctc ccatgaattg aggacaccag ttacttcaat taagggcttt gctgaaacca
     4621 ttaaggggat gtcagctgaa gaagaagcgc tcaaggatga ctttctagac attatttaca
     4681 aagaaagttt gcgtcttgag catattgttg agcatcttct taccttatct aaggctcaac
     4741 aaatgcctat acaatggacg accctttctt tggctgaatt tgtacaggat ttgactcaaa
     4801 gcttgcaacc tcagctcaag aagaaggatt tacagctaaa ggtgcaggtg ccagatgatg
     4861 tcaccctcgt atcagatagt caattacttt cccaaatctt actcaatctt ttatccaatg
     4921 ccatccgtta cactgaacaa gggggaaaaa ttgaggtcaa gacccaaaag gtgaacgaag
     4981 gcattaagat ttctgtatca gatacaggga ttggtattag tcaattagag caggatcgta
     5041 tttttgaacg tttttaccga gttaataaag gtcgaagcag acaaactggt ggcactggtc
     5101 ttggccttgc cattgtcaaa gaactcagtc aattattagg tggccaagtc acggtgacga
     5161 gtcagcttgg cagaggcagt tgcttcacga tttttcttcc taaccaatct ttcgcacagg
     5221 actaataatt tttaaagcta tttttaggag cagccatttt tcaaaagtgg ctttttttga
     5281 ttctttacac aatctttaca tgaatcaggg ataaaccttt acaaagactt gttagactat
     5341 aaatgtagta agcctacaca agaaaaatac atagagataa aggtgattat tatgaaattc
     5401 aaaaaaatgc ttactcttgc agccattggc ttatcaggat ttgggcttgt tgcctgtggc
     5461 aatcagtcag ctgcttccaa acagtcagct tcaggaacga ttgaggtgat ttcacgagaa
     5521 aatggctctg gaacacgggg tgccttcaca gaaatcacag ggattctcaa aaaagacggt
     5581 gataaaaaaa ttgacaacac tgccaaaaca gctgtgattc aaaatagtac agaaggtgtt
     5641 ctctcagcag ttcaagggaa tgctaatgct atcggctaca tctccttggg atctttaacg
     5701 aaatctgtca aggctttaga gattgatggt gtcaaggcta gtcgagacac agttttagat
     5761 ggtgaatacc ctcttcaacg tcccttcaac attgtttggt cttctaatct ttccaagcta
     5821 ggtcaagatt ttatcagctt tatccactcc aaacaaggtc aacaagtggt cacagataat
     5881 aaatttattg aagctaaaac tgaaaccaca gaatatacaa gccaacactt atcaggcaag
     5941 ttgtctgttg taggttccac ttcagtatct tctttaatgg aaaaattagc agaagcttat
     6001 aaaaaagaaa atccagaagt tacgattgat attacctcta atgggtcttc agcaggtatt
     6061 accgctgtta aggagaaaac cgctgatatt ggtatggttt ctagggaatt aactcctgaa
     6121 gaaggtaaga gtctcaccca tgatgctatt gctttagacg gtattgctgt tgtggtcaat
     6181 aatgacaata aggcaagcca agtcagtatg gctgaacttg cagacgtttt tagtggcaaa
     6241 ttaaccacct gggacaagat taaataaaat gtttgctcca taaatctcta aagagatgca
     6301 gacgtttcat cgtacaataa gataaagaag gcaagtaggg aggtgtcgta tctcccttac
     6361 tttcttcact agaaaggaca agatgtgata aaacaagcct tcaaagaagc agtttttagg
     6421 gcaatttttt tcatgagtgc aacagtagct gttgtagcta ttttgctaat ctgtttcttt
     6481 atttttagta atggcttacc tttcatggct aactacggct ttgcccgttt tttattaggc
     6541 agtgattggt cgccaacgaa cattccggca agctatggta ttttaccaat gatcgttggt
     6601 tccttattaa ttaccttagg agcgattgtg attggggtgc caacaggcat cttgacatcg
     6661 gtgtttatgg tttattattg tccaaagccc gtctatggct tcttaaaatc agctatcaac
     6721 ttgatggcgg ccattccatc tattgtttat ggttttttcg gcctacaatt attggtgcct
     6781 tggattagaa gctttttagg aaatggcatg agtgtcctaa ccgcttcgtt actattagga
     6841 ataatgattt tgccaaccat tatcagtttg tcagaatctg ctatccgaac agttcccaaa
     6901 acgtattatt ctggtagctt ggctctagga gctagtcatg aacggagtat ttttagtgtc
     6961 atcttgccag ctgcgagatc tggtatttta tcagcagtta ttttaggcat cggtcgcgca
     7021 gtaggtgaaa ccatggcagt tattttggtg gcaggcaacc agccgattat tccaagtgga
     7081 ctcttttcag gaaccagaac cttaacaacc aatattgttc tggaaatggc ttacgcatca
     7141 ggtcagcata gggaagccct tattgcaacc tcagcagttc tctttttcct tattctcttg
     7201 attaatgcct actttgccta cttgaaagga aaatcatctt atgagtaaat acctgctaaa
     7261 acttctcgtt tattgttttt cagctttaac ctttggctct ctctttttaa tcattggttt
     7321 tatcctcatc aaaggtttac ctcatctaag tctatccctc ttttcttgga cttatacttc
     7381 tgagaacatt tcccttatgc cagcgattat ttccaccgtt attctggtct ttggtgctct
     7441 tcttttagcc ttgcccatag ggatttttgc tggtttttat cttgtggaat atacaaaaaa
     7501 agattccctt tgtgttaaaa tcatgcgatt ggcctcagat accttgtctg ggattccttc
     7561 cattgttttt ggtctgtttg gcatgctctt ctttgtagtc ttcttaggtt ttcaatactc
     7621 tctgttatca ggaatcttaa cctcagtcat catggtgttg ccagtcatta ttcgctcaac
     7681 agaagaagcc cttttatctg ttagtgatag catgcgtcaa gcaagttatg gacttggggc
     7741 aggtaagtta cggactgttt ttagaattgt tctaccagtt gccatgccag gtattttagc
     7801 tggagtgata ctagctattg gccgtatcgt tggtgagaca gctgccctca tgtatacatt
     7861 aggtacctct accaatacgc caagtagtct catgtcttca ggtcgttctc tagccctgca
     7921 tatgtatatg ctgtcaagtg aggggctaca tgtcaatgaa gcctatgcta ccggtgtgat
     7981 tttgattatt actgttttaa tgataaatac tctatcaagc ttattatctc gaaaacttgt
     8041 gaaaggagct tcctagtatg ggaacatttt cagtcagaca cctagactta ttttacgggg
     8101 attttcaagc cttaaaaaat atttcgattc aattaccaga aagacagatt actgccttga
     8161 taggcccatc tggttgtggc aaatcaactt ttctaaaaac ccttaaccgg atgaacgatt
     8221 tggttccttc ttgccatatt gaaggccaag tcctcttaga tgagcaagat atttatagta
     8281 gcaaattcaa ccttaatcag ctacgtaagc gtgtagggat ggtttttcaa cagcctaatc
     8341 cctttgccat gtctatctat gataacgtgg cttatggccc aaggacacat ggtattcgag
     8401 acaaaaaaca attagatgcc ttagtggaga aatctttaaa aggggcagcc atttgggaag
     8461 aagtcaaaga tgatcttaaa aagagtgcca tgtccttatc tggcggtcag cagcaacgcc
     8521 tttgcattgc gcgagcttta gcagtagaac ctgatattct gttaatggat gagccgactt
     8581 cagccttaga ccctatctcc actttaaaaa ttgaagacct cattcagcaa ctaaaaaagg
     8641 attatacgat tatcattgtt acccataaca tgcaacaagc ttcacgtatt tcagataaaa
     8701 ctgctttttt cttaacagga gaaatttgcg aatttggaga taccgttgac gtgtttacca
     8761 atccaaaaga tcagcgcaca gaagactata tttcaggacg gttcggataa ggaaggaaaa
     8821 acctatgaga aatcaatttg acttagaatt gcatgaatta gaacaatcct ttttaggact
     8881 agggcaactt gtccttgaaa cagcttcaaa agccttactg gccttagcct ccaaagacaa
     8941 ggagatggca gagctaatta tcaataagga tcatgctata aaccaaggtc aaagcgctat
     9001 cgaattgacc tgtgcccgtt tgttggcctt gcagcagcca caagtgtctg accttcgatt
     9061 tgtgattagc atcatgtctt cttgttcaga ccttgaacgt atgggagacc atatggcagg
     9121 cattgccaaa gctgttttgc aactaaaaga aaatcaacta gcccctgacg aagaacagtt
     9181 acaccaaatg ggtaaattat ccctcagcat gctagccgat ttattggttg cctttccttt
     9241 gcaccaagcc tcaaaagcta ttagtattgc tcaaaaagat gaacagattg accaatatta
     9301 ttatgcctta tcaaaggaaa tcattggact tatgaaagac caagaaacct caattcccaa
     9361 tggaactcaa tacctttata tcatagggca tctggaacgc ttcgctgatt acattgctaa
     9421 catttgtgaa cgcctagtct acctagaaac aggagaacta gtggatttga attaattcaa
     9481 ctaatcctta aaagagaaga gtacgattaa gtgctctttt ttatggttgt aaaaaagttc
     9541 atttgaccaa tttaagcagt gtagatagtg aggagctgtt tcaattctat cgtgaacgag
     9601 ggaatgctga aaactttatc aaagaaagga aagcaggatt ctttggggat aagacagata
     9661 gttcgaccat gattaagaat gaagtacgta tgatgatggg ctgtctggct tataatctct
     9721 acctcttttt aaagcagcta gctggtgatg aagtaaaggc cttgactatc aagcgttttc
     9781 gacgtctctt ccttcatatt gccggaaaat atgtctctac tgctagacga catattctca
     9841 aattctcaag tctatacgcc tattcaaaac agtttcaagc cttatttgat acaatctgcc
     9901 agataaatct gatactccct gttccatata gagctagagg gcaggggaaa actgcctaac
     9961 agaataagtc accttatttt aaaaatcgag catcaaacca agggaggagt ctgccctttt
    10021 ttaggaaaaa atcaagacaa atctcctcaa tcatgtctcg aacatcagaa attaagcaaa
    10081 atcaccagaa ggacagtatt tcaactagct tttctggtaa tttttgaact gtgtagttcg
    10141 ctagtgctag atatgaataa tttgggatga taaatctttc ttcctcaggt agcctatcat
    10201 aatactcttc aaaaatctta tcaaaaacac tctctttctt ttgggcgata gtttcatctt
    10261 cgtatgtagg agtcctcatc aagaaatact tcaattctag gtattcctta tccaactcta
    10321 tataacttgg catcaacttg taatcttcaa cctccaaacg ttcagcaata tattttaact
    10381 ttgttagtgt tggtctggat tctccatttt caattctaat taattgacgg atacttaatt
    10441 cagactcatc accacaaaat tctgaacgac tgattttttt agccaaacgt aatcttttaa
    10501 ttttttcgcc aaactctcgc aacctacaag aacttcctga gttgtttacc tctattataa
    10561 gcatatactg aatcaaacta tctatcagat ttcttctcac tttaactaaa gactaagagt
    10621 ttatcccttc atctcggtct ttgtgtattt ttccaccata ccccagtaat gcaagtgcaa
    10681 aatcccctag aatatgatag aataagagaa agaaatctat caaggaggaa atcatggaaa
    10741 aacaaaccgt cgccgtcttg gggcctggtt cttggggaac cgccctttca caagtcttaa
    10801 atgacaatgg acacgaggta cgtatttggg gaaatcttcc cgagcaaatc aatgaaatta
    10861 atacacacca tactaataag cactacttta aagatgtcgt tctagacgaa aatatcattg
    10921 cctacaccga cttagcagaa acattgaaaa atgtggatgc gattttgttt gttgtcccaa
    10981 caaaagtgac acgacttgtt gcccagcaag ttgcacaaac cttggaccat aaggttatca
    11041 tcatgcacgc atcaaaggga ttagaacctg atagccataa acgattatca accattcttg
    11101 aagaagaaat tcctgaacat ctccgtagtg atatcgtcgt tgtttcaggg cctagtcatg
    11161 cagaagagac cattgtgcgt gacctaactt taataactgc tgcttctaaa gatttacaaa
    11221 cagctcaata cgttcaggag ctatttagta atcactactt ccgactttat accaatacgg
    11281 atgttatcgg ggttgaaact gctggtgctc ttaaaaatat tattgctgtc ggtgctggag
    11341 ctttacatgg tcttggattt ggtgataatg ctaaggcagc catcatcgct cgaggtttag
    11401 cagaaatcac ccgcctaggg gtagcactcg gggccagtcc attgacctat agcggcttat
    11461 ctggtgtggg agatttgatc gtaacgggaa cttccatcca ctctcgtaac tggagagctg
    11521 gagatgctct cggacgagga gaatccctag ctgatataga agctaatatg ggcatggtaa
    11581 tcgaaggaat ttcaacgact cgagcagcct atgaactagc ccaagaactt ggagtctata
    11641 tgcccattac acaggctatt taccaagtta tttatcacgg aaccaatatc aaagatgcca
    11701 tttatgacat catgaacaat gaatttaaag cagaaaatga gtggtcttaa ccctctatag
    11761 aaaggatttt tatgacatca aaagttagaa aggcagttat ccctgctgct ggactaggaa
    11821 ctcgattttt accagcaacc aaggcccttg ccaaagaaat gttgccaatc gtagacaaac
    11881 caactatcca gtttatcgtg gaagaggctc tcaaatcagg tattgaagat attctagttg
    11941 tcactggtaa atcaaaacgt tctattgagg accacttcga ttcaaacttc gaattggaat
    12001 ataacctcaa agaaaaaggg aaaacagatc ttttgaagct agttgataaa acaactgaca
    12061 tgcggctgca ttttatccgc caaactcatc cacgcggtct cggagatgct gttttgcaag
    12121 ccaaggcttt cgtcggaaat gaaccttttg tcgttatgct tggtgatgac ttgatggata
    12181 tcacagacga aaaggctgtt ccacttacca aacaactcat ggatgactac gagcgtaccc
    12241 acgcgtctac tatcgctgtc atgccagtcc ctcatgacga agtatctgct tacggggtta
    12301 ttgctccgca aggcgaagga aaagatggtc tttacagtgt tgaaaccttt gttgaaaaac
    12361 cagctccaga ggacgctcct agcgaccttg ctattatcgg acgctacctc ctcacgcctg
    12421 aaattttcca aatcctcgaa aaccaagctc caggtgcagg aaatgaaatt cagctgacag
    12481 atgcaatcga caccctcaat aaaacacaac gtgtatttgc tcgtgagttc aatggggctc
    12541 gttacgatgt cggagacaag tttggcttca tgaaaacatc catcgactac gccctcaaac
    12601 acccacaagt caaagatgat ttgaagaatt acctcatcca acttggaaaa gaattgactg
    12661 agaaggaata aaataaaata aaaagataag gttcaaaaaa tgccttatct tttttcatta
    12721 cacaaatttt ctttcaactt cgtcacattc ccattccctt gtagaaaagc aaaatggcga
    12781 gtcctacgaa caagactacc gctcctaatc tctggctggt gttatacatc cgtttttctc
    12841 ctctaactgg aaagataact gctagaaatg cgccaccaac tgcaccaccg atatggcctg
    12901 ctaggctgat tcctggaatc agaacacttc caataatgtt aaccacaaaa agtgtcagat
    12961 aggattgccc tagctgttgg atataaggat tgcgagttgc atagcgaaga acaataatcg
    13021 cggcaaatag cccataaaga gaggtagagg cgcctgctgc taaggattta ggactaaata
    13081 caaaaacaaa gagattgccc atcattcctg ataaaagata gagaaagaaa aactgcttag
    13141 aaccgaaaat ctcctctacc tgccttccaa gataataaag tgaaagcata ttaacaatga
    13201 aatgttccca cccaatatga acaaaaatgg cagacaagag acgccaaacc tgctcgggaa
    13261 agaggcgaat agctggccca tacatggctc caaatcgaaa taatgtatct gccctgtcaa
    13321 agtttccgcc tgcagtgacc aacattagta aaaataccaa ggccgtcact aagaggaaga
    13381 aactcgtcac agggtaacgt ctatcaaaga tttccttcat caattaatac ctcctgaaca
    13441 ggaatatcat ggttttcagg tataaagtcc tgaatttgac aaggatgaac cgtactcaaa
    13501 gtatgaccag aaaaatgttc cagatagcgg tcataataac ctccaccata tccaatccga
    13561 tatccatccg tcgtaaaagc caggccagga acatgaatca aatcaatctg agatgcatcc
    13621 accacttcca aatctccctg tggctccagt aaggcaaagg cagtttttac caactgctgc
    13681 ggatcataga ctacaaagtc catgcgcccc ttgggataag ttttgggtat taaaaccttc
    13741 ttgccgtcct tcagcgcctg ctcaatcagt tcccgcgttt gaaactcatg agaaaaagag
    13801 aggtaggttg cgatgacctt ggcttcttga taaaaggggt gttgtaaaat tcgctcggtt
    13861 aaagcttggt ctatagcctg tttttgctct tgagatatag ccttcatttc atgcaagact
    13921 tgcttgcgta attccgattt catagacaag ccctctattc tgctgccttc tttttcagga
    13981 aactagacac cgcagccacc ccaatagcta agacttcttc cttaggactc atttgagggt
    14041 gatgaagagc gtagggacta tcgataccta gccaaaacat cacgccatca acctttgaaa
    14101 ggagataacc aaagtcctcg cctgtcatag caggttcgat atcaatcaac tcgattccgt
    14161 ctttttcgtc aaagaagtcc atcagttcac gcgccaaggc tggattgttc tcaacaggta
    14221 ggtatccacc ttgtttgagt tccacttcga cttccatatc aaaggcagct gcaacccctt
    14281 ctgcaactgt ttttaccctc ttttgcacca agagactcat gtcctgtgtc aaggcacgaa
    14341 tagttccatg taaaaaagct gtgtctgtga tgacattgtt ggtggttcca gcttgaaaaa
    14401 cgccgaaggt caccactgct ccctcgattg ggttgacatt gcggctaaca actgactgca
    14461 cttgggtcac aaagtaacta gccgccacca aggcgtcatt ggcttcatga ggaaaagctg
    14521 cgtggccacc tttgcctttg aaacggatct tcacctcgca agttcctgca aagagtgtat
    14581 gagtattagt cgcaatctgg ccgactttca aatctggacg gacatggagg ccataaaatt
    14641 gatctggcaa ccaatctcca aaagcatcgt cctcatacat gagcatacca ccagcttcat
    14701 tttcttcagc aggctgaaat agaaagagca gattattctt gggttgctcc tcaagggcgc
    14761 gctcaagaca gcctaaggca atggtcatat gaaaatcatg gccacaggca tgcatgcgac
    14821 cttggtgttg agaagcaaaa ggtagacctg tttgttcgac gataggcagg ccatcaatat
    14881 ctgtccgcca accaatggtt cgctccggct gacttccctg caggtagacc aaaatccctg
    14941 tccgccaagt acgaatttga acaaaatcct tgcccgtagt caatttctca atcacatcca
    15001 gcaaataagc ctgagtcttg aactcctcca agccaatctc tggaatctgg tgtaaatctc
    15061 gtctagtctg aatcaaatct aacatctatc tgtcctccga tatagcagaa agaggctgga
    15121 aaaagggttc cgcctctttt ttacttttac aattacaagg tacgaagcgc atcctctagc
    15181 gctgtttttt gttgagtttg ggcatcaatt tctttgataa tacgagctgg aacacctgct
    15241 actaccacgt tttctgggac atcttgggta acaatagctc ctgctgcgac aactgaacca
    15301 ctaccgattt ggactccttc gataaccact gcattagccc cgatgagaac attgtctccg
    15361 acacggactg gttcagcact agctggctca atcacacctg ccaaaactgc acctgcacca
    15421 acgtggctat tttttccaac gatggcacgg ccaccaagga tggcacccat gtcaatcatg
    15481 gttccagcac cgatttcagc accgatattg ataacagctc ccatcatgat aacagcattg
    15541 tcaccaattt ccacctggtc acggataata gcacctggct cgatacgagc gttgatagca
    15601 cgcttatcta gcaaaggaac tgcagaatta cgagcatctt gctcgacaac ataatcttga
    15661 ttttctacca aaccttcaag aagcggagcc acgtccttcc agtctccgaa taggacattt
    15721 cctagtttga caacagagct aggcacagca gttgcgagtt gcccctcaaa ggttactttg
    15781 acactggttt tcttttcagc attggcgata aattggataa tttcttgagc gttcattttt
    15841 gtagcagtca taggtgcctc ctggttcgtt ataatgatac ctattctacc aaaaaaggcc
    15901 tcaaatttga aacctttttg tctgttttta ggtatgattt tttagggatg tgagataaaa
    15961 cgtgctgtcg gtaaaagcaa gcccctaccg atacaaccta aaaacactct tcacaactat
    16021 ctccccgtgt tttattgact attctagtat agcacacttt tagaattaag aaaagacttt
    16081 ttatctactt tctttcgctt cttctataga caaaaatatc atgggtaaaa taatcaaggc
    16141 tatagctaga aggagggacc aatccactac taatcctaag aacaaaacac tcaagagagc
    16201 agaagagaga ggttcactgg cactgataac ggcaaccacc aaaggagaaa ccaaggacac
    16261 agccttcatg gaaatgaaaa aagcaaaagc cgttccaaag aaagcgataa tgaggcaaat
    16321 caagatactc caaatatcaa gagtaaagga aagctgataa accggcgaga ggacattgct
    16381 aaacaaacct gccaaaatca tcccccaccc aaccgtagga acaaaaccat aacgcttagc
    16441 aaaaggttgg ggcaagataa cattaaacat aacacccatg gcactcagca aacctgttat
    16501 aagagctagc ggcgtcatgg ataactgaga gaggtctccc tttgtcgcca tcaagcaaac
    16561 acccagcatg gcaaccaaaa catagaaaac agcgcttttt gacgctcgtt tttgataaac
    16621 caagcgattg taaaagagga taaagacagg gctaataaac tgtaaaatag ttgctgtcgt
    16681 agcatttgag tattctacac agagatagaa aaaatactga actgaaaaaa tccccaaaat
    16741 agcataggct aaaaagggca ggtaattttt cttgtctcgc caaatatcta gcacttgcga
    16801 ttttaattgt attgcagacc aaatgagtac aagactccct gccagtgtca aacgcataga
    16861 ggtaatccag cccgaagaca cctgataatg agtaaagaag tactctccta aaattccaca
    16921 gattccccat attaagccgg ataggagcga ataaattttt ccgttaacaa tctttttctg
    16981 atactgattc atacccttat tgtaacacga aaagaaaaaa gtggcaatcc attgattggc
    17041 cacttcattt agagaattat cgtctcgccc ttgaagaaga aggtcgtgta gtacttgagt
    17101 tactgctatc gctagaacta ctacttgaac tgctggagct ggatggagtt ggtagactcc
    17161 ccacaatact agaccaagca ttctgataat ccgcatcact tccgccaata gcaaagcgat
    17221 aacttgtcgc tggcgctcct gacttattag cccaatagct ggtaacagtc gaacctgtga
    17281 cttctacctc ttttccttca acagaaacct tccctggttt ttgacctgtt gatttcaaga
    17341 cttccgattt cactacacta ggatctaaag caaagcgctc gttcccccaa atgcttgggg
    17401 aagcttgctg aatcgcattt accagatgag ccatgtaatt agagttatta gaataacctg
    17461 ctctacgtga caatgaatga ttatcatcat gcccaatcca gccacctagg gttaatctag
    17521 gtgtcgaaag catgagccac atattttcgt cttggttggt tgtaccagtc ttcccaatcc
    17581 aatctgcatt agccagagta ggatttaaag aagtcaggtt agacttgaag gttgttgtca
    17641 cacgagagga tagaacttct cgtagcaatc cctgcataat cgtcgcagta gcttttgaat
    17701 agacttgaac cggtttatcc tgatactcat acaccactct accatctgct gcttcaatct
    17761 ttgaaatcac atgcttctga tgataaactc cattattagc taaggtctga tagccattgg
    17821 tatgctgggc aactgtgact tcaataccac cacccattgg caagctctca ataccgtact
    17881 caggaatctc gtaacccatc ttttccatat aacccttgac atcaacaccc ttttcacgga
    17941 gcatacgata ggtccagtaa gcagggatat tccatgaata gttcagagct tctcccaagg
    18001 tcatcattcc tgttcccttg ctattagcat acataatcgg attgccatta gcaaagtttg
    18061 ttggatagtt agatagaatc gtttcacttc ccatcaagcc ctggtcaata gcaataccgt
    18121 aggccagcaa gggcttggta gtagaagctg gcgaacgttt ggtatcaaag gcatgattat
    18181 tttgattttc ttgataatta cgaccaccta caaagcctag aatagcacct gtttggttgt
    18241 ccatcaagac attccctact tctacacgac ctgttccatc gtctaaaaga tagccataat
    18301 cagcaaccgc actttgcatg gcagaatgaa ttttctgatc tatggtagta gtaatcttat
    18361 aaccaccatt ttcaatttcc ttggctgcca aatctcgata aaacttctga gttgcctcat
    18421 ttttcaactc cttagcggag acattgtctc tctgagctag atagtcatac atacgttctt
    18481 gagcttctgc caaagttgta aagtataaat agtctcgtga aattcctgta accgtgcccg
    18541 atggtaaaaa gtcctgttta aggtcataat ccttgtactg agaatactcg tctttgctta
    18601 atgcacctgt acgatacata ctgtaaagaa ctgccttagc ccgtcttaag ccaatttcta
    18661 ggtcttcatc actcttcaac tccccagtat tttcataagg agagtaagta atgggactct
    18721 gtggaagtcc tgctaaaaat gctgcttgag gaacagtcaa ctgactggca tctacaccga
    18781 aaattccctc agctgcttgc cgagcccctg caatattctg tcccttatta tttcggccaa
    18841 agggagccac attgagatag gtcgttaaaa tctcatcttt attcatggcg cgttccaagg
    18901 caagagcatc cacaatctct gccgccttac gagccaaggt cggcgcatcc ccaaccacct
    18961 gctgtttaat tagttgctgg gtcaaggttg aacccccact agaggaaccc aaacctacaa
    19021 atttccccaa ggtcgcacga atcaccgcct tgggtactac acccttatgt tctttaaagt
    19081 gttcatcttc tgtcgcaatg atagccttct tcagattttc cgaaatttgc tcagatgaga
    19141 tagaagtgcg caacaaatca ctctctatgg aagcaatcac cgtcccgtcc gaataggtaa
    19201 tctctgaaat agaagagatg tccttgacct gattcaccaa ttcttctgtc tgaggcaccc
    19261 gaaccttgtc aaataaggcc actccgtacc ccaaagcaat cccagctccc aacattcctc
    19321 ctagaaaacc gagtacaaag agtaagttaa ataaggcttt tatactcagt aaaatagctg
    19381 ggaaaatggc tgacttatct aaggttttag attttttggt acttgaacct ttcttgccag
    19441 gtctagctga ttttttattt ttttgttttt gctggaaaaa ttccagcatt tttcgtttta
    19501 attcatttaa ttgattttgc atggatttcc tcactttatc tattatacca caaaagggaa
    19561 attttcaata aaatagccac tttcttccct attctgctag gctattgccc aagtttgtga
    19621 tacaataggt agaaacaata attttaaaaa ggagaaaaaa cacatgcaca tttttgatga
    19681 gctaaaagag cgtggtttga tatttcaaac gactgatgaa gaagctttgc gtaaagccct
    19741 agaagaaggt caagtttctt attatactgg ctacgatcca actgctgaca gccttcacct
    19801 aggccacctt gtcgcaatct tgacaagtcg tcgcttgcaa ctagcaggtc acaaacctta
    19861 tgcgctcgtt ggcggtgcta caggtctcat cggagatccg tccttcaaag atgctgaacg
    19921 tagtctccaa acaaaagaca cagtagatgg ctgggtcaag tctatccaag gacaactttc
    19981 tcgttttctt gactttgaaa atggcgaaaa caaggctgtc atggtcaaca actacgactg
    20041 gtttggcagc atcagcttca ttgacttcct ccgtgatatt ggaaaatact tcacggtcaa
    20101 ctacatgatg agtaaggaat ctgttaaaaa acggatcgaa acaggaattt cttacactga
    20161 gttcgcttac caaatcatgc aagggtatga cttcttcgtc cttaaccaag accataatgt
    20221 cactcttcaa atcggtggtt ctgaccagtg gggaaatatg acagctggta ccgaattgct
    20281 tcgtcgtaag gcggacaaga ctggtcacgt tatcactgtt ccactaatca cagatgcaac
    20341 tggtaagaaa tttggtaaat cagaaggaaa tgccgtctgg ctcaatcccg aaaagacttc
    20401 tccatacgaa atgtaccaat tctggatgaa cgtgatggac gctgacgctg ttcgcttctt
    20461 gaaaatcttt actttcttgt cacttgatga gattgaagat attcgtaaac aatttgaagc
    20521 agcgccacac gaacgcttgg ctcaaaaagt cttggctcgt gaagttgtta cacttgttca
    20581 cggagaagaa gcctacaaag aagcacttaa catcactgag caactctttg caggaaacat
    20641 caaaaacctt tctgtcaaag agctcaaaca aggacttcgt ggtgtgccaa actaccaagt
    20701 acaggcagac gaaaacaaca atatcgtgga actgctcgtc tcatctggta tagttaactc
    20761 aaaacgccaa gcccgtgaag acgtccaaaa cggagccatc tacgtaaacg gcgaccgcat
    20821 ccaagagctt gactatgtct tgagtgacgc tgataagtta gagaatgaac tgactgttat
    20881 ccgtcgtggg aagaaaaaat actttgtatt gacttactaa actattcaac atttatctat
    20941 aaacaaagga gttaacctcg agaaaggtaa ctcctttttg ctgttaataa ctctcatcta
    21001 tctattttta atagacaggc tacgcaggac aatgcgcaag gttgttagat tatgtaagat
    21061 agagagattt gaaggactga gccaattaaa taagccgaag ccaatcaaac tactatttac
    21121 gacaacggta tcttgaatat ttttcttgat gagtgtttgc aaagatgatg ataacgaatc
    21181 caactcttgg aagaaatcca aacgattatc taacaataag atatcactca tctgcttaga
    21241 aatatctgca ctctcattca tcaccacacc gatatctgat agagttaaag ccgctgagtc
    21301 attcaatcca tctccaacca tcaaaatagt gtgacctgct ttctgcagtt tctctactaa
    21361 ctcaaatttc ccatcaggtt tcaagtctgt atagacctga tcaaagggca aatctttgac
    21421 taattccttt gtcctaacca aggtgtcccc tgttgccaga atcaattttt tcccctgtgc
    21481 cttaagttta tccaaggctg tttttgcttc ttttctcaaa ggagtatgaa tgcagaacat
    21541 tccaatcaat tcattttgat aagccaagaa taagagattg tagtgactct tgtactcttc
    21601 aattaaagca ttttgttctg aactgatatg aatctgctca tcctgcatca agacataatt
    21661 cccaataaga actggttggc catctatatg agatttgatc cccttgcttg cgatatattg
    21721 gagtttccca tgcatttcct catgttcaat tccctctatc tcagcttgct tgacgatggc
    21781 attagcaata ggatgataaa tgtgttcctc aagacaggca ctgattctga gaatatcttc
    21841 ctcactatag tctccaaaag gtaacacctt ttcaactata ggataactag ttgtgattgt
    21901 tcctgtctta tcaaacaaga aagtatcaac ttccagatat ttctccagaa catctccatc
    21961 cttaatcacc atttcacggt tcaacccttc cttgataact gtcaaataag ctacaggagt
    22021 agagattttc aaagcgcagg agaaatcgac caataggaaa gaaatagcct tagaaaaaga
    22081 acctgtcaat aggtaagtca gcccagcccc caagaaatta tatttgacga ctttatccgc
    22141 catcttgatg aaatagcgtt gtttcgtttt cttgttttct tcagatttct tcatcaactc
    22201 aatcagctgt aaaatacggc tgttcatctg attatctgtt acacgaatgc gtaactctcc
    22261 agtttctaat actgtatttg cacaaaccaa atcagactct cttttttcaa ctggaaaact
    22321 ctctcctgtc aaggaacttt cgttgaccat acctaaacct gaaactactt gtccatcaaa
    22381 cagaatttca tttccttgag ataagatcaa gacatctcct atttgaacat cggaactctt
    22441 gatactaaca accgtatcgc cctgtactag gaatacatcg ctctcttttg caagaagact
    22501 ctgttctaaa tctgttgcag tttttttcaa ggaccactga tctaaatgat tccccaaatc
    22561 aagcataaac atgatattgc tagctgtctt ggattggttc ataaacaaag acaataaaat
    22621 agccgaacag tccaagactt ccatcgttag ttccttacgc gctagtgttt gataggcttc
    22681 tctaatataa cccaaagcct gataacaagt ccatatatag cgaataggat acggcacaaa
    22741 actacgaaaa agtacacgct taaccgctgc acctgaaaca atagaataag cactctcttc
    22801 tctacgaatg ggaagagtca tcaactcaga aactttccct ttatcaattc tttttaaaaa
    22861 ggcttctgca ttatctaata cagaaaagcc ttcttttatg cgtagagtaa agtgctgttg
    22921 atccatgtaa aactggatag actcaatccc cttttcatct ctcgccaagg aacgaagata
    22981 gtcttgaata tccaaggtaa gtgaaaaaga agatgatagt cggatatgtt ggtatcctct
    23041 atgtagcact ttaaaagaca tattattcac ctataaggct atctaattgc tcttcttttt
    23101 tctcttgctc gtacaaatat ttggcatctt gcaagacatc gtctccatgt tgcttcacaa
    23161 cagaaacaga tgcatctagc tcgtctttca acttgtaagc cttagccaaa gctttagaat
    23221 aacctttttt agcttcctta cttgctaaga ttttcaaacc aagggtacca aatgcgacac
    23281 cacccaaaaa taatgaagat tttttcgcaa cttttgcaac ggttaatact tcttttaaca
    23341 tacttatttc ctccttatca ttattatata gcaatttaga tataaaagca atttcattct
    23401 ataaattgga gaattagttt tatttctact gaatctccca tctacttatt cctaaagttg
    23461 ctttcatttg cctcttttga tacacttaaa ctatgaatac aaatctcaag cccaaacttc
    23521 agcgttttgc ttctgcgact gcctttgcct gtcctatctg tcaagaaaat ctgactctgt
    23581 tagagactaa tttcaagtgc tgcaaccgtc attcttttga cttggcgaaa tttggctatg
    23641 tcaatctagc acctcaaatc aagcaatctg ctaactataa caaggaaaat tttcaaaacc
    23701 gtcaacaaat cctagaagcc ggcttttacc aagctatctt agatgctgta tctgacttgc
    23761 ttgcaagctc aaaaactacc acaacaattt tggatatcgg ttgtggtgaa ggattctatt
    23821 ctcgcaaact acaagaaagt cactctgaaa aaactttcta tgcctttgac atctccaaag
    23881 attcagtcca aatcgctgct aaaagtgaac ccaactgggc agtcaattgg tttgttggcg
    23941 acttggcacg acttcctata aaagacgcta acatggatat tctgcttgat atcttctcac
    24001 ctgccaacta tggagaattt cgtcgcgttt tatccaaaga cggtatcttg ataaaggtta
    24061 tcccaactga aaatcacctc aaagaaatcc gtcaaagagt acaggaccag ctgacaaaca
    24121 aggagtattc taaccaagat atcaaggagc atttccagga acactttacc atcctatcta
    24181 gtcaaactgc ctctctgact aagactatca cagcagaaca actccaagcc ctactcagta
    24241 tgactcctct cctctttcac gttgaccaga gcaagattga ctggagccaa ctgacagaga
    24301 ttaccatcga agcagagatt ttggttggga aagcattcta aactagactt cccaaaggta
    24361 taaaacttac cgaaaatctt caatattata aaaacgcata agaacaggta cacaaacctt
    24421 aatcttatgc gttttgttat agtttccata cacattaagg ggaaatagtt gaaatcagtt
    24481 ctcttaataa ccattgaata ccatatacta catttacccc aaatatgata taggccgtat
    24541 aaatcaagta tttcgatttc tcccgtctca aagaataaat cttgtacaaa atagataaat
    24601 ttaaaataat aaataataga gacaaaatct tcctccaaaa tcaatattaa aagtagccag
    24661 tcaatcatag ttctaatctt tattttcgat tcgaaattac aaacttatca atgaacaata
    24721 agtgatataa ggccagtacc agaccccaaa cgctaccacg aaaagagaat tcttgatgat
    24781 tgagagtaaa caaatcatgg ttaccaatat agaggcaggt caaaataaca aataaaatcc
    24841 aaaataaatc cgtccattta tattttgaaa aatatttttt taacataata gcatattttt
    24901 ttgactataa taacagtata tgataaaatg taagcagata caaataaaaa ggagaaaagc
    24961 tcatgaacaa attaatgaaa tttatttcgg tttttttgac gtcaattgtg ttaattgtat
    25021 cagcgattcc aagtgtttca gctgtatacg cttctgaaca agtatcacaa attgaaacaa
    25081 atatggaact tcaacctgtc acttctctaa cagaagaaca aatcaataca cttgcaaacg
    25141 aaatccaatc ttttcatcca gacgtctcac aacaatggat caaagaagta attaaccgac
    25201 aattacaagg cgattataca atcccaccta catactctcc atttagagca gcttggcaag
    25261 gtattacagt taatcaaatg ggtgctctat tagatactgc aatagcttta gcattaggag
    25321 gaactactgc aggccttgca aatctaatta aagtaaaagg aaaacatgca gcaaaaagtg
    25381 ctattcgttc agcaatttct agatatctag gtagttggtt tgtaaatgat gttgctttag
    25441 aattcgctat gaatttatta tcaccgggga cttatttagc acaattatgg gataaaaatg
    25501 atgccattcc taacaacgga aggattaact tttaatcatg agatatacaa taaaattaat
    25561 tgggtataac gtattttcgt tgtttatatt ctttatctta tcaatgttgt ttaaagttcc
    25621 ctttaggtgg tggatcagca ttgcaatgac gacttcgtcg tacattgtat ataattactt
    25681 aaagaaaaag agagaaaagg aatgatctta taccagatca ttccttttct gctttctgta
    25741 aatccctcgg agtgtgtttg gcgctttgat acgaagtgtc aaagctgtat aaacgttact
    25801 atttccaaaa aagtaacaga aaattcctgc tttgtaggac tgctttgggt gagtaataga
    25861 catgtagact caccctatct cctgatggcg aaaagaattt agcaatgatg caagttgttc
    25921 tgaactcgag aaaaaacatg ccgaaatcga agaaaagcgt gcagaacttg aagcgaagaa
    25981 gcaagaaatg ttgaagctct tggataaata gaatttagaa aacagaaaaa actaaaaaaa
    26041 tgtagggttt atttccctac attcttaaaa taacttagta cataaatgac aatgctgcca
    26101 ctgccgtcaa tattgcaaaa actattttcc aaattttttt attttttcca aaaatcttat
    26161 caggtaacaa gcttattaca agaaaggtaa taaacaacac caatttaagt gtgtatatca
    26221 taaaaacacc ttctttccta caattatatt ataatacttt atgtaagcga atacaagaga
    26281 tatgaggtaa ccaactacga aaaaatataa ggctgaagac agtaaaaacc agcatttaaa
    26341 gccttcactg tatctaggat ttccctattt ttagacaaga ttgacccaaa tccacagcat
    26401 ttaaaaaagc gaatttcaat cgaaactcgc ttttttacaa aatgtattag tataaatttg
    26461 tatcttatac tcaatgaaaa tcaaagagca aactaggaag ctagccgcag gttgctcaaa
    26521 gcaccgcttt gaggttgcag ataaagctga cgtggtttga agagattttc gaagagtatt
    26581 agttcaagtg ccagatgtct tcattatact gagcgattgt acggtcagat gagaagaatc
    26641 ctgctttaga aatgttaacg atgactttat ccaaccatgc gtcacggtct tcgtagtcag
    26701 caagcatttg ctctttgact ttgatgtagt cttccaagtc aagaagagtc atgaaccagt
    26761 ctttgttgat caattcattg taaagacgtt ccaagcgctc tttgtttcca gctgcaagaa
    26821 ctgcatcact aacgatgaag tcaaccaatg gtttgatagc ttcacgagcg tagaattcgc
    26881 ttgatttgta agctgctttt gcgtaaaggt cgataacagt ttctgaatct tcaccgaaga
    26941 tgtagatgtt ttcttctcca accaactcag cgatttccac gttagcaccg tccatagtac
    27001 caagtgtcaa agctccgttc aacatgaatt tcatgttacc agtacctgaa gcttctttag
    27061 aagcaagtga aatttgttct gagatatcac atgctgggat aaggaaactt gctgcagtaa
    27121 cgttgtagtt ttcaaccata actacttgca agtgtggagc tactgctgga tcgttagcaa
    27181 taacttctga catgcaaagg attaaatgga taatgtcttg agcgattgtg taggctggag
    27241 ctgctttacc accaaagaag attgtgattg gacgagcagg gatgttacca gctttgatgt
    27301 caaggtattt gtggatcacg tacaaagcgt tcatttgttg gcgtttgtac tcgtgaagac
    27361 gtttgatttg gatatcaaag atagaatttg gattgatttc cacaccttgg tgttctttca
    27421 agtgacgagc caatttacgt ttgttgtgag ccttgatgct ttccaatttt tctttgacag
    27481 ctgctttgtc ttcataagac aacagttttt caagctcatc tgcttcatgg tgccaaccat
    27541 ctccaagaat ctcatccaag tagtgagaca atcttgggtt agcatgcata agccaacgac
    27601 ggaaagtgat accgtttgtt ttgttgttga acttttctgg gtaaaggtcg tagaaggctt
    27661 tcaactcaga atttttcaag atttcagtat ggagtgctgc aaccccgtta acactgtatc
    27721 cgtagtggat atccatgtga gccatgtgaa cacgtccgct ctcatcgatg atttgaacag
    27781 ctggatcttt gtactctgcc ttcacacgac ggtccaattc ttcgatgatt ggtaccaagt
    27841 gaggaaccac ttcttgcaag aattcaagag gccatttttc aagcgcttca gcaaggattg
    27901 tgtggttagt gtaggcagtc atgctacgaa caattgagat tgcttcgtca agatcgatac
    27961 cacgtgcagt caaaagacga atcaattcag gaatcaccat tgatgggtga gtatcgttga
    28021 tttggacaac tgcgtagtca gcaaggtcat gcaagttgct tcctttttcg attgcttcgt
    28081 cgatgatcaa ttgcgcaccg tttgaaacca tgaagtattg ttggaagata cggagcaatt
    28141 caccttgacg gtcactatca tctgggtaaa ggaagagagt taagttgcga gcgatatctg
    28201 tcttgtcaaa gttgatacca tctttaataa tagaagaatc aactgaatcc aagtcaaaca
    28261 aacgcaagcg gtttttagtc gctgtttcat aaccagtaac atcaatatcg taaagagttg
    28321 atgtcaaagt aaagtctgca aatggtactt ggtagctacg gcttgagcga accaaccagt
    28381 tttgctctgt caaccatgca tttggaattg tttcttgttg gttgttttta agaacttgtt
    28441 ggaaaagacc aaagtggtag ttaagaccaa caccgtcacc attcaaacca agagtagcaa
    28501 ttgagtcgat aaagcaggca gccaaacgtc ccaaaccacc attaccaaga gatggttcca
    28561 attcaacttc ttcaacttcg atcaagtctt tacctgcagc tgcaagttct tttttaacat
    28621 cgtcgtaaag accaaggtta atcaagttgt ttgacaagag tttaccaatc aagaactcag
    28681 ctgagatgta gtaaactttt ttcttaccag tgttgactgg tttttggctg cttgcaagct
    28741 tgctgtagtt aagaagagca aggtaaagct cttcattgct acattctgca atggttttat
    28801 tgtaacgatt ttgtacaaat tcttgtagtg ataacatgtt tagttgtctc ctgattattg
    28861 tcttatttct ttaaatctac caaattttca ttaattcggc gataaattgt tgtcaagtca
    28921 agcaaacctt cctcgacagc tggtgtcaat tgatcttcag tcatacgcca agaccagttt
    28981 ccaccaaggg tagatgggaa gttcatacga gctgcctcat ccaattctag taaatcttgc
    29041 atagttgcaa ttgccataaa gctaactgat gaaaatactg tacgaagcat agcgtgtacc
    29101 actgtttcgt attctttacg gttcgtgtaa cgagccatgt actcacgagt cgcatcatca
    29161 atctcattac ggtaccaacc aagaaccgta ttgttatcgt gtgttcctgt gtacataact
    29221 gagttagcag gtgccaagtg tgggctatca atgctttcgt cttctgggtt gaaggcaaat
    29281 tgaagaatct tcattcctgg gaagccagta cgttcacgca attcgatcac ttcatctgtc
    29341 atgaagccaa ggtcttctgc gatgatgttt agctcaccaa gttcttcctt aacggctgca
    29401 aaaagcttgt agcctggacc tttcacccac tcaccaggtg ctgctgtatc ggaaccagca
    29461 gggatttccc agtaagattc gaagccacgg aagtggtcga tacgaacgat atcgtagatt
    29521 ttgaagcttt cacgcaagcg ttcaatccac catttgtagc cgtctttgtc cattgcttcc
    29581 cagtcataga ttgggttacc ccaaagctga ccagttacag aaaactcatc tggtgggcat
    29641 cctgcgatac aagtagcctt accattgaca tctgttttga agagatgtgg atttgcccac
    29701 atatcacttg aatcttccgc tacgtagatt ggcatgtccc caacgatttc gatgtggttg
    29761 tcgttagcgt aagctttcaa tttcaaccat tgttggaaga agaagtattg agtcacacgg
    29821 tggtaaacca acttgtctgc caattgctca cgatagcttt caagtgctga agctttacga
    29881 gcacgagcat ctgcatctgg ccattcagtc caagcaagat tgtcaaaata ctctttgata
    29941 gccatatact cagcaaagag ctcaagccat gattggttgt cttgagcaaa tttctcaaaa
    30001 tctttaacat ctccgacttc aaagaaacgt ttcaccgctt tttctaaaag aggacgacgt
    30061 gcatagtaga ttttagcata gtcaacttca gacgcatcgc taccaaagtc aactccttca
    30121 aggtcacttg cttccaacaa accttgctcc accaagatat ctaaatcgat aaaatgagtg
    30181 tttcctgcga aggctgagaa agattggtaa ggagaatccc cgtaactagt tgctcctaat
    30241 ggaaggattt gccagtaacg ttgttttgta cggaccaaga aatcaacgaa gtcgtaagca
    30301 ctttgaccaa atgatccgat tccgtaagct cctggaagag aagagatgtg catcaacaca
    30361 ccactttgac gttttttcat aatagcacct cgtgtgttaa aataatggaa cgttgcgtat
    30421 tttgcagacg caaacgtttg cgttacttat aagtatactc cttttcaagc ataattgcaa
    30481 gcgtttttaa aaaacttttt tgattatttt taaggagact aactcatttt agcaaagata
    30541 acttaaaact atctaatcct aaggtttgac tctcacgaga cagtctacaa agtacaaaac
    30601 ctccttaggg agcttgaatt tatatgtaac aaagcacaaa cgtccataga aaatagatag
    30661 ggattagaac cagggaggta gcccctcctg gtttccctct ttagacagat tccatatatt
    30721 tttattaaaa atactgtatt cttatctatt tcatactttt cttctattaa aatgcaaaat
    30781 tatgctatca aatcaagctt aaaaattttt taaaattttt acaaaaaata cttgcaaccg
    30841 ttttctattt gtgctatact aagctcataa aggaaaacgt ttgcgtttcc ttatcaataa
    30901 aacttgctat tctttgggag gaatacacta tgtcatctaa atttatgaag agcactgcgg
    30961 tgcttggaac tgttacactt gctagcttgc ttttggtagc ttgcggaagc aaaactgctg
    31021 ataagcctgc tgattctggt tcatctgaag tcaaagaact cactgtatat gtagacgagg
    31081 gatataagag ctatattgaa gaggttgcta aagcttatga aaaagaagct ggagtaaaag
    31141 tcactcttaa aactggtgat gctctaggag gtcttgataa actttctctt gacaaccaat
    31201 ctggtaatgt ccctgatgtt atgatggctc catacgaccg tgtaggtagc cttggttctg
    31261 acggacaact ttcagaagtg aaattgagcg atggtgctaa aacagacgac acaactaaat
    31321 ctcttgtaac agctgctaat ggtaaagttt acggtgctcc tgccgttatc gagtcacttg
    31381 ttatgtacta caacaaagac ttggtgaaag atgctccaaa aacatttgct gacttggaaa
    31441 accttgctaa agatagcaaa tacgcattcg ctggtgaaga tggtaaaact actgccttcc
    31501 tagctgactg gacaaacttc tactatacat atggacttct tgccggtaac ggtgcttacg
    31561 tctttggcca aaacggtaaa gacgctaaag acatcggtct tgcaaacgac ggttctatcg
    31621 taggtatcaa ctacgctaaa tcttggtacg aaaaatggcc taaaggtatg caagatacag
    31681 aaggtgctgg aaacttaatc caaactcaat tccaagaagg taaaacagct gctatcatcg
    31741 acggaccttg gaaagctcaa gcctttaaag atgctaaagt aaactacgga gttgcaacta
    31801 tcccaactct tccaaatgga aaagaatatg ctgcattcgg tggtggtaaa gcttgggtca
    31861 ttcctcaagc cgttaagaac cttgaagctt ctcaaaaatt tgtagacttc cttgttgcaa
    31921 ctgaacaaca aaaagtatta tatgataaga ctaacgaaat cccagctaat actgaggctc
    31981 gttcatacgc tgaaggtaaa aacgatgagt tgacaacagc tgttatcaaa cagttcaaga
    32041 acactcaacc actgccaaac atctctcaaa tgtctgcagt ttgggatcca gcgaaaaata
    32101 tgctctttga tgctgtaagt ggtcaaaaag atgctaaaac agctgctaac gatgctgtaa
    32161 cattgatcaa agaaacaatc aaacaaaaat ttggtgaata aaaaatttgt tcaagggggg
    32221 tggaaatcaa atcccccttt gaatttatca atagagacac aaataattta gctttcttat
    32281 aaaaaagtag tatcctatga aaggagttaa tatggaaaag caacaaccta gtaaagcagc
    32341 cctgctgtct atcattcctg ggttaggaca gatttacaat aaacaaaaag ccaaaggttt
    32401 tatcttcctt ggtgtaacca tcgtatttgt cctttacttc ctagcacttg caacccctga
    32461 attgagcaac ctcatcactc ttggtgacaa accaggtcgt gataattccc tctttatgct
    32521 gattcgtggt gccttccatc taatctttgt aatcgtttat gtactctttt atttctcaaa
    32581 tatcaaagat gcacatacga ttgcaaaacg cattaacaat ggaattccag ttccacgcac
    32641 actcaaagac atgatcaaag ggatttatga aaatggcttc ccttacctct tgatcattcc
    32701 atcttatgtt gccatgacct tcgcgattat cttcccagtt atcgtaacct tgatgatcgc
    32761 ctttaccaac tacgacttcc aacacttgcc accaaacaag ttgttggact gggttggttt
    32821 gaccaacttt acaaacattt ggagcttgag taccttccgt tctgcctttg gttctgttct
    32881 ttcttggact atcatttggg ctttggcagc ttctacttta caaatcgtaa ttggtatctt
    32941 cacagctatc attgccaacc aaccatttat caaaggaaaa cgtatctttg gtgttatttt
    33001 ccttcttcct tgggctgtcc cagccttcat cactatcttg acattctcaa acatgtttaa
    33061 cgatagtgtc ggtgctatca acactcaagt attgccaatc ttggctaaat tccttccttt
    33121 ccttgatgga gctcttattc cttggaaaac agacccaact tggactaaga ttgccttgat
    33181 tatgatgcaa ggttggctcg gattcccata catctacgtt ctgaccttgg gtatcttgca
    33241 atctattcct aacgaccttt acgaagcagc ttatattgac ggtgccaacg cttggcaaaa
    33301 attccgcaac atcactttcc caatgatttt ggctgttgcg gcacctactt tgattagcca
    33361 atacaccttc aactttaaca acttctctat catgtacctc ttcaatggtg gaggacctgg
    33421 tagtgtcgga ggtggagctg gttcaaccga tatcttgatc tcatggatct accgtttgac
    33481 aacaggtaca tctcctcaat actcaatggc ggcagctgtt accttgatta tctctatcat
    33541 tgtcatctca atctctatga tcgcattcaa gaaactacac gcatttgata tggaggacgt
    33601 ctaagatgaa taactcaatt aaactcaaac gtagactgac tcaaagcctt acttaccttt
    33661 acctgattgg tctatcaatt gtaattatct atccactgtt gattaccatt atgtcagcct
    33721 ttaaagcagg taacgtctca gcctttaaac tagatactaa tatcgacctc aattttgata
    33781 actttaaagg cctcttcact gaaaccttgt acggtacttg gtacctcaac actttgatta
    33841 tcgccttaat taccatggct gttcaaacaa gtatcatcgt acttgctggt tatgcttaca
    33901 gccgttacaa cttcttggct cgtaaacaaa gtttggtctt cttcttgatc atccaaatgg
    33961 tgccaactat ggccgctttg acagccttct tcgttatggc gcttatgttg aacgccctta
    34021 accacaactg gttcctcatc ttcctctacg ttggtggtgg tatcccgatg aatgcttggc
    34081 tcatgaaagg ctacttcgat acagtgccaa tgtctttaga cgaatctgca aaactagacg
    34141 gtgcaggaca cttccgccgc ttctggcaaa ttgttcttcc acttgttcgc ccaatggttg
    34201 ccgtacaagc tctctgggcc ttcatgggac ctttcgggga ctacatcctc tctagtttct
    34261 tgcttcgtga gaaagaatac tttactgttg ccgtaggtct ccaaaccttc gttaacaatg
    34321 cgaaaaactt gaagattgcc tacttctcag caggtgctat cctcatcgcc cttccaatct
    34381 gtattctctt cttcttccta caaaagaact ttgtttcagg acttacaagt ggtggcgaca
    34441 agggataatt tatccccgcc accctttttc attttatact cttcgaaaat ctcttcaaac
    34501 cacgtcagct ttatctccaa cctcaaagtt gtgctttgag caacctgtgg ctagtttgca
    34561 ctttgatttt cattgattat tagcaattgt cactgtaaat aatatccttg tagcaagcaa
    34621 tttttctcct agacttgaaa taaagcgcat ttctctatat aataatactt atatagaaaa
    34681 caccttttag aaagatacct atgcttccat atccattttc ctttttttca agtattttgg
    34741 ggtttcgtaa gcccctgtcc aaacgtttcg ggctcaactg gtttcaactt ctctttacca
    34801 gtatcttcct tatcagcttg tctatggtac ccattgctat ccaaaacagc tcccaggaga
    34861 cctatccgct agaaactttt atcgataatg tctatgaacc tctgacaaat gaggttgtcc
    34921 aggatctctc tgaacatgct acaattgtcg atggcacatt aacttatact ggaacagtta
    34981 gtcaagcctc ttctgttgtg attggtccaa gtcaaatcaa ggaattacct aagaacttgc
    35041 aactgcattt cgatacaaat gagctagtca tcagcaagga aagcaaggaa ctgacccgca
    35101 tctcttaccg agccattcag actgagagtt tcaaaagcaa agacagcttg acccaagcaa
    35161 tttctaaaga ctggtaccaa caaaatcgtg tctatatcag cctcttccta gttctcggtg
    35221 cgagcttcct ctttggtttg aatttcttta tcgtctctct aggagctagt cttctccttt
    35281 atatcactaa gaaatcacgc ctcttttcat ttagaacctt taaagagtgc tatcacttta
    35341 tcttgaactg tttaggattg ccgactctga ttacacttat tttgggatta tttggccaaa
    35401 atatgacaac cctgattact gtacaaaata ttctttttgt tctgtatctg gtcactatct
    35461 tttataaaac acatttccgt gatccaaatt accataaata ggagattttt atgcccgtta
    35521 cgattaaaga cgtggccaag gctgctggtg tttcgccttc aaccgtaacc cgtgttattc
    35581 aaaataaatc aaccattagc gacgaaacaa aaaaacgcgt tcgcaaggct atgaaggaac
    35641 tcaactacca cccaaacctc aacgctcgta gcttggtaag cagctatact caggttatcg
    35701 gattagttct tcctgatgac tcagacgcct tctaccagaa tcctttcttt ccatcggttc
    35761 tacgtggcat ctctcaagtc gcatctgaaa accactatgc cattcagata gcaacaggga
    35821 aagatgagaa ggagcgtctc aacgctattt cacaaatggt ctacggcaag cgtgtagatg
    35881 ggctaatctt tctctatgcc caagaagaag accctctcgt aaaactcgtc gcagaagaac
    35941 agttcccctt ccttatccta ggtaaatctc tatctccttt catcccactt gtcgacaacg
    36001 acaatgttca agctggtttt gatgcgactg aatatttcat caaaaaaggc tgcaaacgca
    36061 ttgcctttat cggaggaagt aaaaagctct tcgtgaccaa agaccgttta acaggctatg
    36121 aacaggcgct taaacattac aaacttacca ctgacaacaa tcgcatctac tttgccgacg
    36181 agtttctgga agaaaagggc tataaattta gcaagcgatt attcaagcac gatccacaaa
    36241 ttgatgctat catcacaacc gatagcctcc tagctgaagg tgtttgtaac tatattgcca
    36301 aacaccagct ggatgtccct gttctcagct ttgactcggt taatcccaag ctcaacttgg
    36361 cagcctatgt cgatatcaat agtttagagc ttggtcgtgt ttcccttgaa actattctcc
    36421 agattattaa tgataataaa aacaataaac aaatttgtta ccgtcaattg atcgcccaca
    36481 aaattatcga aaaataagag actgggcaaa gagtcgttaa aagcaaaaac gcatactatc
    36541 aggtattgaa aaaacttgat actatgcgtt ttattgtggg aagatttact tccttttcta
    36601 ctgaaattga gtcttttccc aagatctttt tatactcaat gaaaatcaaa gtgcaaacta
    36661 ggaagctagc cgcaggttgc tcaaaacact gttttgaggt tgtggataga actgacgaag
    36721 tcagctcaaa acatggtttt gaggttgcag atagaactga cgaagtcagc tcaaaacatg
    36781 gttttgaggt tgcagataga actgacgaag tcagtaacat atatacggca aggtgaagct
    36841 gacgtggttt gaagagattt tcgaagagta ttaatcacta attatctatc tcaacaaatc
    36901 ttcctagaat atgaacattt tccgagacag agacaaagga gcttggatcc acttgtgtca
    36961 taatctgttt aaattcatta aactctgcac gtgtaatgac agtgattaaa actgcctttc
    37021 tctcgtgatt ataggttcct tctgcatcgt ggatcatggt tgctccgcgg tgcaattttt
    37081 tatggatttt ttcaattacc ttctctggat gatttgtcac aatcatggcc tgcatacgct
    37141 tttgcttagt aaagactgcg tctgtcacac ggctagagac aaagatggta atcatagaat
    37201 aaagagcgta tttccaacca aaggtcaaac ctgctatcag catgatagtt ccatttacca
    37261 agaaagaaat actaccgaca ttcttacccg ttttcttgcg aatagtcagg ctgacgatat
    37321 ccgtcccacc actggagata ttgtttcgaa gagcaaaacc aatccccaaa cccataacaa
    37381 cacccccaaa aagggaattg ataatgggat cctccgtcaa ggttgccaca gggacaaact
    37441 ggataaagaa ggaactcata gataccgtga taaaggtaaa gacggtgaac ttatggccaa
    37501 tctgatacca agctaagacc atcaaaggga agttaatggc gtagaagctt agcgaaatcg
    37561 gaatatgaaa accaaaccag tgattactca aggcagagat aatctgtgcc agacctgttg
    37621 caccactcga atacacatgc cctggttgga aaaagaaatt aactgctact gctgataaaa
    37681 aaccatagac cagagaggcc gaaatcttct catcatactt ttctcgagag atactttgta
    37741 agacacgtaa aatttttatc tgataagcaa agcggcgcag ataatagcgc caccgcttaa
    37801 ttcgttttgt ttgtttcatc ttcttctact tgtaagctga gttcctctag ttgtttgaga
    37861 gcgactgttg atggagcttg tgtcattggg tcagttgcct tgttgttctt aggaaaggca
    37921 atgacttcac ggatattttc ttctccagca agcaacatga caaaacggtc aagcccgata
    37981 gccaaaccac cgtgtggtgg gaaaccatag tccatggctt caagaaggaa accaaactgg
    38041 tcattggctt cttcagttga gaaaccaaga gccttgaaca tgcgttcttg aaggtctttt
    38101 tggttgatac gaaggctacc accaccaagc tcataaccgt tcaagacgat atcgtaagca
    38161 atggcacgaa ccttagccaa atcaccttct aattcgtgag cagtctcttc ctgtggaagt
    38221 gtgaatggat ggtgggcgct catgtagcgg ccttcttctt cagaccattc aaacatcggc
    38281 cagtcaacca cccaaaggaa gttgaactta tcattatcaa tcaagccaag ctctttagca
    38341 atacgtccac gaagggcacc cagtgttgca ttagccactt caagcgtatc cgccacaaag
    38401 agaaccaagt ccttatcttc aagagcaagc gctgttgtca attcttcttg gataccagtc
    38461 aagaacttgg caactggtcc gtttaattct ccatcaacca ccttgaccca agcaagacct
    38521 ttggcaccat actgtttggc tacttccgtc atcttgtcga tgtctttacg tgaatagttg
    38581 tccgcagctc ctttgaccac aatcgctttt acagcaggtg cttctgaaaa gactttaaag
    38641 tctacacctt tgaccacttc tgtcaagtcc tgaagcaaca tgtcaaaacg agtatctggc
    38701 ttgtcagaac cgtaaagagc catagcatca tcgtatttca tacgagggaa cggtagcgtt
    38761 acttcgatgc cttttgtttc cttcatcacg cgcgcgatca agccttctgt aatatcttgg
    38821 atttcttgct cagtaaggaa ggacgtttcc aagtcgacct gagtaaattc aggctggcgg
    38881 tctccacgca agtcctcgtc acggaaacat ttaacgattt ggtagtaacg gtcaaaacca
    38941 gcattcatca agagctgttt cgtgatttgt ggactttgag gaagagcgta aaaatgcccc
    39001 ttattaacac gagacggcac taaataatca cgcgcccctt caggcgttga cttagaaagg
    39061 aatggtgtct ccacgtcgat aaactccaac tcatccaagt agttgcggat agagtgggtc
    39121 accttggcac gaagtttaag attttccaac atttctggac gacgaaggtc aaggtaacgg
    39181 taacgcaaac gtgtatcgtc atttgcctca atgccatcct taatctcaaa tggtgttgtc
    39241 ttagctgtgt taagcacaat aagagctgtc acgtttaact caaccgcacc agttggcaac
    39301 ttatcattgg cttgctcacg cgcagcgacc tgaccagtca cctcaataac aaattcgcta
    39361 cgaaggcttt cagctgttgc cataacctct gcagatactt tttcagggtt gataaccaac
    39421 tgcatgattc cttcacggtc acgaagatcg ataaagatca aaccaccaag gtcacgacga
    39481 cggccaaccc atcctttcaa ggttatttct tgtccgatgt gttcctcacg aacacgacca
    39541 gcatacatac tacgtttcat tatttctctc ctcttttatt ctgttactat tttaccataa
    39601 aagcgcaggc tcttcatgaa aatcatcaga aaagtttgcc agtctttaaa agtcaggtga
    39661 aagccctaaa aattagcgct aatactcttc gaaaatctct tcaaaccacg tcagcgtcgc
    39721 cttaccgtag gtatggttac tgacttcgtc agtttcatct acaacctcaa aaccatgttt
    39781 tgagctgact tcgtcagttt catctacaac ctcaaaacca tgttttgagc tgactttgtc
    39841 agtttcatcc acaacctcaa aacagtgttt tgagcaacct gcggctagct tcctagtttg
    39901 ctctttgatt ttcattgagt ataatacaaa aatccgatga acttcaccgg actcttttat
    39961 tttgaatttt tgcctgcttt acgcttttca gcgatttcgg ctgcctttcg aggcaagaca
    40021 atttccgtta tgtaagccgt cccaaaacgc agtacacctg caataggagc aaagacaact
    40081 gctagatagt tatagaagaa atcgcctttg aaggcataag ctagcgctcc aatgatgaaa
    40141 aatagaacga ctgcctgaat cactgctaat aaaattactc gtttcatgtg acctcctgac
    40201 tctattatag catgagaatc atcaaaaagc cgactaaatt attcaaagcg tgaagagaaa
    40261 tactgtagac cagacctttt ctgctaatgt aagccaaacc caaactaaaa ccaaggctaa
    40321 aatagacaaa aaattgttgc acatcacctg gaaaatgaat caaggcaaat agaagactag
    40381 ataccagaag aaaaatcagg gttcgtttac tattgtcctg cttaggaaag agatagcgtg
    40441 ctaacatccc tctaaaaaca atctcttccg tcaaaggagc aaaaataacc acagcaaaga
    40501 atgagaaaag tggttgagac aaggtcaagt ctgtcgctat ttgctgattt actgaaggat
    40561 catctggcaa gaagaattga acgaccagag ataagaacca aaccaagaca ggaagccaaa
    40621 taaatcgatt aaagccgctc ttctcaatat gaacaggagc cttctgatac catttgtaaa
    40681 tgccgtacac atatactcca gcaaaggcca catagagtag agtaacagca tagggtgaag
    40741 cgcctaaagc aagcgacgca gtcgcgagcc cctgaataaa gccatagata aataaaaagg
    40801 atagaagggc tagaagaatc cagccaaggt ttttaagtaa tttcatagat aactccttta
    40861 tttgaaataa cgttttacca taggtaactg catcacattg atataaacat ggatggctcc
    40921 tacaagcaag aaagctagta actgaatctc tcctgtcaag aaagaaatga taataagaaa
    40981 aatatataag gctggtaaga catattggtg taattggaat aaaattcgaa aactctgttc
    41041 caaattagcc tgacgctccc cttcatcata agaatttata tagttcaaga catcctttgg
    41101 tgtagcgaaa aattccaaat caaactgacg aacaatcgca atggttttaa aaagagattt
    41161 ttgagcgact aagaatacca caaagagtaa gaaagaaagg aaaaatgttt gagggtttgt
    41221 atgcaatata atcacctcac ttaatgaaat aaaaatagcc aatggaatcg ctacacctgt
    41281 aatattaaaa gcaatggttc caaactcaag attccgatac atttgcacat aataggtttc
    41341 attcagatcg tcatccattt cctcttgata caaggaatga aattttctgc ttttctttaa
    41401 gaaattgaaa gtcaaaaaca tactaatgaa acctatcagt aaacaaatag ctgatatcca
    41461 tggcatcaag gcttttacat ctaaaataat ttcgtgggat tcgacacgtg ccttaaacat
    41521 ccctacaaac atgcccaaga caatagacat caaaaataac aatctacgtt tctttttcat
    41581 attcattctc ctttttcact tgctagattt ttggatttct tttcaatcca ttcaattact
    41641 gggatgagag caaagtagac ccaaacaaat tggtcgcttt gatagagatt aaaccagctt
    41701 aggtccatcc caatcagtag aaatacactg actaataaag ctatgaccac tacataataa
    41761 atcactttat acttgttcat cactcgtcct cctccaaacg aaataccgat tcgactgttt
    41821 cgttgaaaat ttgagatatt ttcagggcaa tgataatgga tggggtgtac tcatcccgtt
    41881 ctagtaggct aatggtctgt ctggaaaccc ctgccagttt ggctaggtcg gtttgattga
    41941 gaccatcgcg agctctaagc tcttttagac gatttttaag ttgcatgtta cacacctact
    42001 ctccgtcaaa ttcaacggtt tggatatcct caatgcgttg caacttgaat ttttcttttc
    42061 ccgtattatc tacacgtcgt agctttaccc attcctcatc aacatccaca acttcccagt
    42121 tatctggccc aatatacact cccgttataa ttggttcctt tccaatcatt tcttgaaata
    42181 atctcgacat ttctgcgttt cctttctctt ttcgctcaag ttttttgatt ttattctcta
    42241 gtttcttgat ttttttagaa ttattagaat aaaagaaaat cataaatagt ataaatccta
    42301 gtacccacat catagctcct ttcttctttt ctcttaactt gcttacatcg taacacatct
    42361 ttttcttttt gacaagtata gttgtcaaaa agtttatgat ttttgtcatt ttgcaaagga
    42421 aaaaggtcag gagtaagttc ctgaccactt tatctatcat taatactctt ctaaaatctc
    42481 ttcaaaccac gtcagcttca tctacaacct caaaaccatg ttttgagcaa cctgcggcta
    42541 gcttcctagt ttgctctttg attttcattg agtataaaat cctagttttt caaagatttc
    42601 tgagaagttt tggctgattg tctcaagtga cacttgcact tcttctcggg tttggttgtt
    42661 cttgaccgtc acttgtccgc tttcgacttc gctctctcct agggtgatga gggtcttagc
    42721 cgcaaagaca tctgctgact tgaactgagc tttgagctta cggttgaggt aatcacgctc
    42781 tgctttgaaa ccttgttggc gaagagcttg gactagttct agggctttga catttgctcc
    42841 atcacccaag actgcgatat agacatctag ggcgttttcg atagggaggg ccacgccttg
    42901 tttttcaagg atgagaagca ggcgctctac accaagccca aaaccaaatc cagcagtttc
    42961 agggcctcca aagtaagcaa ccaaaccatc gtagcgacca cccgcacaga ctgtcaagtc
    43021 attgccctca atctctgtga taaactcgaa aatggtgtgg ttgtagtagt ccagaccacg
    43081 caccatattg gtatcgatga tgtaatctac tccaagattt tccaacatct gacgcacagc
    43141 atcaaaatga gtttggcttt cttcatcaag aaagtccaag atagacggcg cattctctac
    43201 tgccaccttg tcttcttttt ccttagagtc caagacacga agaggatttt cctccaagcg
    43261 acgttggcta tccttagaca aggtctcctt gagcggtgtc aaatagtcaa tcaaggcttg
    43321 gcggtaggct gcacggctct caggatttcc aagagtgttg aggtgcaatt tgacaccttg
    43381 aataccgatt tccttcaaaa aataggctgc catagagatt gtttccacat cggtagctgg
    43441 attgctagag ccaaaacact caacaccaat ctggtggaat tggcgcaagc gccctgcctg
    43501 tggacgctca taacggaaca taggtcccat atagtagaac ttgcttggct tttgcacttc
    43561 tggggcgaag agtttatttt ccacatagga acggacaacg ggcgcagttc cttctggacg
    43621 gagggtaata tgacggtcac ccttgtcata aaaatcgtac atttccttgg ttacgatatc
    43681 cgttgtatct ccgacagagc gactgataac ctcgtaatgc tcaaaaatag gcgtgcgcac
    43741 ttctgcatag ttatagcgct tgaaaatctc acgggcaaag ccctcaacgt actgccactt
    43801 agcagattca gcaggtaaaa tatcctgcgt tccttttggt ttttgtaatt tcatagggaa
    43861 tcctctttaa acttaatagt cttattttac cacaaataaa gggattaaaa cagtaagaaa
    43921 aaaattagga tttagatatc atttttgaga ttaagaattg tcaaaaaaat agctagcaag
    43981 gaaagaccaa caaatagcat ccaagtcaac tgtatattcc atacggctac tagtgaaaaa
    44041 caagctgttc ccacaggtat ggataaggta aacaatagac ctaaaaaatt actagtacga
    44101 gctagaacct ctggagctag atttttcatg agcatggcac taatctttgg ttgaacttta
    44161 ccagacacat acagggtaaa gaagagaaat agcaaaccaa gcacgacttg attgaataaa
    44221 ttagccaaac caactagact aagtcctacg gtcgcccaca tcatcaatct aggcaaggac
    44281 tgcttcccaa aataatcatt gcccgtaagg ctactgatga tgattgctac taaaacacag
    44341 aattgattga taaatagtgc ctctgtataa gaaaaattca agagagaatg gctcaaaaag
    44401 aagatattat aaattccacc caaagcgcca cccaaggaat taataagcaa gacagcaaag
    44461 agcataaaac caaagttttt ctgcccactt ttaagaaaaa cgagacgtaa atttcggtaa
    44521 attgttagga actggtcttt gatagaaagc ttctcatttt ttaagttttc accatcagca
    44581 gatgacattg acaggctcaa tttgcttttt cctaaaaaga ggatagtggc tgatactagg
    44641 aaaaagcagg cattgattcc cgcaacgaga gaaaaattgt tgaccgatag agctaagagc
    44701 cagactccga aagcttgacc accaatagct gaaatatagg tgatgaactg tgaaaaagaa
    44761 taagcctcca tcagatcatc ttcagctact ttttccttaa taagaggcat gcgcaagcca
    44821 cctgcaaaat cactgatgac atcactaatg acattgatca aacacaggct agaaaaggca
    44881 aagagactag cttgctgaac aactagggct gctaggaaaa atagaaccgc ctgaaacaaa
    44941 ccgctataga ccatccattt gaccttgtcc ctcgtgtaat ctgcccgaat ccctacaaaa
    45001 actgtaaaga gggtcggaag aatcatgaca atattcgcca tagcaacagc aaaagatgct
    45061 tgtgacaagg tcgatgcata gacgataaag accaggttga aaatcgaagc accaaaagca
    45121 ttgaagaagc gtgataaagt caaaattcta taagctggat ttctaaacaa tagtttcata
    45181 gataatctcc cttcttgttc taagttctac atcaagaata gctttag
//
LOCUS       DAVLVF010000007        44134 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVLVF010000007 DAVLVF010000000
VERSION     DAVLVF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.007"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..476
                     /locus_tag="VYN66_000353"
     CDS             <1..476
                     /locus_tag="VYN66_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1494436.1"
                     /translation="VLDYFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREAL
                     ITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPGKE
                     VEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            514..819
                     /locus_tag="VYN66_000354"
     CDS             514..819
                     /locus_tag="VYN66_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1494437.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            867..2342
                     /locus_tag="VYN66_000355"
     CDS             867..2342
                     /locus_tag="VYN66_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV1494438.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2692..9393
                     /locus_tag="VYN66_000356"
     CDS             2692..9393
                     /locus_tag="VYN66_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV1494439.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTDLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFLYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKNLTVEWEEVPAEN
                     LIKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9641..10497)
                     /locus_tag="VYN66_000357"
                     /pseudo
     CDS             complement(9641..10497)
                     /locus_tag="VYN66_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10708..11067)
                     /locus_tag="VYN66_000358"
     CDS             complement(10708..11067)
                     /locus_tag="VYN66_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV1494440.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMIVELFLMK"
     gene            11159..12115
                     /locus_tag="VYN66_000359"
                     /pseudo
     CDS             11159..12115
                     /locus_tag="VYN66_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            12099..12656
                     /locus_tag="VYN66_000360"
     CDS             12099..12656
                     /locus_tag="VYN66_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1494441.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12649..12903
                     /locus_tag="VYN66_000361"
     CDS             12649..12903
                     /locus_tag="VYN66_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494442.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTYHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12915..14969
                     /locus_tag="VYN66_000362"
     CDS             12915..14969
                     /locus_tag="VYN66_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV1494443.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLSLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            15148..16008
                     /locus_tag="VYN66_000363"
     CDS             15148..16008
                     /locus_tag="VYN66_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV1494444.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLRDPVFKKD"
     gene            16219..16929
                     /gene="ccdA2"
                     /locus_tag="VYN66_000364"
     CDS             16219..16929
                     /gene="ccdA2"
                     /locus_tag="VYN66_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1494445.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16949..17515
                     /locus_tag="VYN66_000365"
     CDS             16949..17515
                     /locus_tag="VYN66_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV1494446.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            17526..18638
                     /gene="msrB"
                     /locus_tag="VYN66_000366"
     CDS             17526..18638
                     /gene="msrB"
                     /locus_tag="VYN66_000366"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1494447.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISEDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18906..19643
                     /locus_tag="VYN66_000367"
     CDS             18906..19643
                     /locus_tag="VYN66_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1494448.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19640..21331
                     /locus_tag="VYN66_000368"
     CDS             19640..21331
                     /locus_tag="VYN66_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1494449.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS"
     gene            21447..22010
                     /locus_tag="VYN66_000369"
     CDS             21447..22010
                     /locus_tag="VYN66_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV1494450.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGHIG
                     KESSQEIGELIVETILDILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKT"
     gene            22032..27701
                     /locus_tag="VYN66_000370"
     CDS             22032..27701
                     /locus_tag="VYN66_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEV1494451.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGYLSGDILKTLGLDTV
                     LEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEALKEEAPKTEESPKEEPKS
                     EIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPTEEPKV
                     EQVGEPVEPSEDEQAPTAPVEPEKQPEAPEEEKAVEETPKPEDKIKGIGTKEPVDKSE
                     LNNQIDKASSVSPTDYSTASYNDLGPVLETAKGVYASEPVKQPEVNSETNKLKTAIDA
                     LNVDKTELNNTIADAKTKVKEHYSDRSWQNLQTGVTEAEKVAANTDAKQSEVNSETAS
                     LKTAISRLNTDKVELENQLKIAQGKTETDFSMESWTVLSTAKNKAQEVKDNGTATQEQ
                     INEAEKSLKTALADLSVDKTALGSAIDTATKKNKENYTNQTWAELETALTAAKSVNTN
                     ESKQSDVNEAAEKLTATMEKLVELSEKPRLTLSIEKRDIDRKATVTYTLENPANTQIK
                     SITATLKKGEEVVKDFVLTEENLKTNHLTALFEKLDYYKEYTLSTDMVYNRGNDDETE
                     SISEELIQLNLKKLELKDIQTVSLMKFENGQESQVTHLSDKPTDLSKLYLKVTSSTSK
                     DAVLAVSSIEEEIIENKKIFKIHADTPELVVRKKDGSLSKGFDYYMERVIPHDGDIYY
                     DFKDLISAMTSNPTGTFILGRDISSRNVKPDGNGKSYIKGEFKGKLLGTNDNVRHSIF
                     DLEYPLFDTIKSGVVKDIDFKHVNMVFPDSNQGDNVATIARVIKDKTKIENVNVEGYL
                     EGRDHVAGLVNNLEGNSEIENVSFTGKIKSKGGNSITAGIAGRNILSRVKRAYVNADI
                     EVHRSSNSSMLVAVNGINADASGGWGTWGRLTESVAKGTLETKQGGQAGGASSTVWPY
                     GAIDNVVSYAKVTKGKELFGSDGDLNYDWFMKKISNIFGVQGISSGDSGSDSKFTRIS
                     EEEANQKVASYNITAPNLMSDSSLLVDRLNESWKNTDQFESIQDYQAQNQLIYQNLTK
                     FTPYYNKEFIVHEGNALTPEQEILKTKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGS
                     QKRYKVTSTGKFSITNLPEYQVEDLNVVYTSEHIVHPLDSSLINNLVEELKKVELYTE
                     STYQVLGIDKDNANKLNRTKRLFLDESLDAVKTQLPTFVKTMFENEWLHINGESSGAV
                     AALRQKIMDNKTAILLALTYINRYYDVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRL
                     GSSGENRLKGSENAETFKQLFASETKQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDY
                     IQFEERPSLVEEIKDAKYRVYDNLTAPYYQGYILPLLTLKNTHLAILSNYSTMTFVSR
                     EKRPNWKNEDFDKWVKYVATAHRNHVDTWYKILPDNIKGKMVKENVTAVWEGLSIPGS
                     EWVDQNAVDRKGRDYAPAREFFNLVGGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSE
                     YGISVFTHELTHVNDTWIYLGGYGRRENMGPEAYAQGLFQSPVPGQPGWGALGLNMAF
                     ERKNDGDLIYNASPTQFENRKELDSYMKNYNDTLMMVDYLEGDAVISKGKEAITKWFK
                     KVEPKVVSQTAQYDTVRQLTAEEKEKLSVSSVDDLVDQGLMSDRAVGNNTYNPADFET
                     SYIAIDYMTGIYGGGKNSVGSPGALMFKHNTFRMWGYYGFEEGVLGYASNKFKQASRD
                     EGHAGLSDNFIISKISKGEFLTMEAFKKGYFKKVVEELKTKGIRPVTINQKTYSTFEE
                     LQEGFKQAVERDLKKNQLDERETRNFKFQVFRQLLQQTDSFKTSIFR"
     gene            27762..29483
                     /gene="pabB"
                     /locus_tag="VYN66_000371"
     CDS             27762..29483
                     /gene="pabB"
                     /locus_tag="VYN66_000371"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV1494452.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFHSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAYPFDPEILRQKIEAECQTCDANQDYRLRISLSKSGEIEFSRQIL
                     TPLSPSFCQAKLCLQEANLNQSFTYFKTTYRPHLSIEEQEKIYHNGKGELLETSIGNL
                     VLKIDGKLYTPPIRLGILPGIYHQYLLETGQVEEKVLTLADFAQAEAIYGCNAVRGLY
                     ELQVIGL"
     gene            29603..31129
                     /locus_tag="VYN66_000372"
     CDS             29603..31129
                     /locus_tag="VYN66_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1494453.1"
                     /translation="MKSKIVLGASLAIATLSLVSLVEIEGLSPFLIENVSANTHSANK
                     VINHKVSIYLENADEGKGLTVNFSTDSVSPNLFDEFEKKSGITITTMLVNAKTGEVVE
                     KRLTPSVFLRSNDLTSGTISSFIFSEYPDGEYKYVVSKGDFIDPKTQFKHQYRGESPV
                     FRIRNRKYVELGTTDKKLDERRDNSVYKDGVVEHKVNLSLTSYQGGNGVTAIFSTDSV
                     NSNLLNSFGEKAKKVLIRSKLINVKTGEVIDETFSPKVSLTSKILKSGSTAVFYFIDL
                     TDGEYKYVAYESQQYTDPQTTLTHQYRGESPIFSIKDGKFSGLVSASKPDENPKPTPK
                     PDEKPKPSAPQQEKTKPTVQSGWVGSSYYQNGKKVTSKWIFDKKYNSYFYLDASGNYV
                     QNAWVGNYYLKSGGYMAKSEWIYDKNYGSYYYLTSEGSYARNTWSGNYYLKSNGKMAK
                     SEWVYDSNYKSYYYLTSEGSYARNTWVGNYYLKSNGKMAVNERTPDGYRVDGSGKWVK
                     "
     gene            31216..32135
                     /locus_tag="VYN66_000373"
                     /pseudo
     CDS             31216..32135
                     /locus_tag="VYN66_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            32270..33229
                     /locus_tag="VYN66_000374"
     CDS             32270..33229
                     /locus_tag="VYN66_000374"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV1494454.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            33326..34165
                     /locus_tag="VYN66_000375"
     CDS             33326..34165
                     /locus_tag="VYN66_000375"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV1494455.1"
                     /translation="MTKADTIFKENIECILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(34213..34383)
                     /locus_tag="VYN66_000376"
     CDS             complement(34213..34383)
                     /locus_tag="VYN66_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV1494456.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            34504..35388
                     /gene="miaA"
                     /locus_tag="VYN66_000377"
     CDS             34504..35388
                     /gene="miaA"
                     /locus_tag="VYN66_000377"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV1494457.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            35381..36619
                     /gene="hflX"
                     /locus_tag="VYN66_000378"
     CDS             35381..36619
                     /gene="hflX"
                     /locus_tag="VYN66_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV1494458.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            36612..37235
                     /locus_tag="VYN66_000379"
     CDS             36612..37235
                     /locus_tag="VYN66_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV1494459.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            37250..38179
                     /locus_tag="VYN66_000380"
     CDS             37250..38179
                     /locus_tag="VYN66_000380"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV1494460.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            38200..38955
                     /locus_tag="VYN66_000381"
     CDS             38200..38955
                     /locus_tag="VYN66_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1494461.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(39009..39917)
                     /locus_tag="VYN66_000382"
     CDS             complement(39009..39917)
                     /locus_tag="VYN66_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1494462.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            39981..40250
                     /locus_tag="VYN66_000383"
     CDS             39981..40250
                     /locus_tag="VYN66_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV1494463.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            40250..40630
                     /locus_tag="VYN66_000384"
     CDS             40250..40630
                     /locus_tag="VYN66_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1494464.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            40719..40946
                     /locus_tag="VYN66_000385"
                     /pseudo
     CDS             40719..40946
                     /locus_tag="VYN66_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40986..41711)
                     /locus_tag="VYN66_000386"
     CDS             complement(40986..41711)
                     /locus_tag="VYN66_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEV1494465.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGKILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTILKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            41873..43714
                     /gene="typA"
                     /locus_tag="VYN66_000387"
     CDS             41873..43714
                     /gene="typA"
                     /locus_tag="VYN66_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV1494466.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            43734..43988
                     /locus_tag="VYN66_000388"
     CDS             43734..43988
                     /locus_tag="VYN66_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV1494467.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLLLAYFSVRDITLMPVKKSKRR"
BASE COUNT        13644 a         8071 c         9741 g        12678 t
ORIGIN      
        1 tggtattaga ttatttcttt gacaaaaacc ttgtgttttg cttagaagcg gataatcaag
       61 aacatctctt tgatcaggtt gcaactttat tggaagaacg agaaattgta actccaactt
      121 atcgtgaagc cttgatcacg cgtgaaaagt catttccaac tggtttagat atggaatttc
      181 taggaaagga cttgccaaat gtagcgattc ctcacacaga tattgttcat aatctagctg
      241 agaaagtggt ggttgttcga ttagaaaaac cagtaacttt tcacaatatg attgcgccag
      301 gtaaggaagt agaagtatct ctactcttct ttatcattaa caattcaagt tcaagtcaaa
      361 cgaacattct ggctcagttg atggactttt tcacaggaaa tggtcatctt gaagacctat
      421 caaaaatttc tgaaccagaa aaactttatg cttacattgc tgaagcaacc gcttaatctt
      481 gtctattaaa aaataaaatc ggaggaaatc caaatgatta aaattcttgc tgcctgcggt
      541 gcaggtgtta actcaagtca ccaaattaaa agtgctctag aagaagaact ttcaaaccgt
      601 ggttatgatg ttcactgtga tgcagtcatg gtgaaagatg taaacgaaga ccttatgaaa
      661 ggttacgata tctttacacc aatcgctgca acagaccttg gttttgaacc aggtatccca
      721 gttatcgaag ctgggccaat cttattccgt atcccagcaa tgagcgctcc agtatttgac
      781 aatattgaag cagctattaa agaacatgga ttaagctaat tctaatttgt taacattcat
      841 ataacataaa aaagggagga actgttatgg atgcaatctt tgacctaatc ggaaaggttt
      901 tcaatcccat cttagaaatg ggtggacctg tcatcatgtt gatcattttg acagtattgg
      961 ctttactttt tggagtgaaa ttctccaaag cgcttgaagg tggtatcaaa cttgccatcg
     1021 ctcttacagg tatcggtgct atcatcggta tgctaaatgg tgctttctca gcatcacttg
     1081 caaaattcgt tgaaaacact ggtatccaat tgagtattac agacgttggt tgggcaccac
     1141 tcgcaacaat cacttggggt tctgcttgga cactatactt cttgctcatc atgttgattg
     1201 tcaacgtagt gatgcttgct atgaagaaaa ccgatacact tgatgtcgat atctttgata
     1261 tctggcactt gtctatcaca ggtctcttga ttaaatggta tgctgataac aatggtgtga
     1321 gtcaaggggt ttcactcttt attgctacag cagctatcgt ccttgtcggt gtgttgaaaa
     1381 ttatcaactc tgacttgatg aaacctacat ttgatgacct tcttaacgcc ccaagttcat
     1441 caccaatgac atcaactcac atgaactaca tgatgaaccc agttatcatg gttttggata
     1501 agatttttga aaaattcttc ccaggccttg ataaatatga ctttgatgct gctaaattga
     1561 acaagaaaat cggtttctgg ggatctaaat tcttcatcgg tttcatcctt ggtatcgtta
     1621 tcggtattat gggaactcca catccaattg caggtgttgc agatgcagat aaatggcgtc
     1681 ttgttatcaa aggatggttg tctcttggtt tgactgccgg tgtatctttg gaactcttct
     1741 cacttatcgg ttcatggttc atcgcagccg tagaaccact atcacaaggt attacaaacg
     1801 ttgctactaa acgtcttcga ggacgtaaat tcaatatcgg tcttgactgg ccattcatcg
     1861 ctggtcgtgc tgaaatctgg gcttgtgcca acgtacttgc accaatcatg ttgattgaag
     1921 cagtgcttct ttcaaaagtt ggaaatggta tcttgccact tgcaggtatc atcgctatgg
     1981 gtgttactcc agctctcttg gttgtaactc gtggtaaatt gctccgtatg attatcttcg
     2041 gaacactctt gttgccactc ttccttcttt caggtacact tattgcacca tttgcaacag
     2101 aacttgctaa aggtgtaggt gccttcccag aaggtgtgag ccaaactcaa ttgattactc
     2161 actctactct tgaaggacca atcgaaaaac ttcttggttg gacaattggt aacactacaa
     2221 ctggtgatat caaagcaatc cttggtgcag tagtcttcct tgtattctat atcggtatct
     2281 ttgcttggta cagaaaacaa atgatcaaac gtaacgaaga gtacgcagca aaagcaaaat
     2341 aatgcgctcc tataaaatat aaactcaatg aaaatcaaag agcaaactag gaaactagcc
     2401 gcaggttgct aaaaacacag ttttgaggtt gtagataaga ctgacgaagt cagctcaaaa
     2461 catggttttg aggttgtaga tgaaactgac gaagtcagct caaaacatgg ttttgaggtt
     2521 gtagatgaaa ctgacgaagt cagtaatcat atctacggca aggcgacgct gacgaagttt
     2581 gaattggatt ttcgcagagt gtaaattgtt gaaactaggt tgtcatacca tgtataccac
     2641 ttgggctttg gtagtagcta actgcactaa atataatata aggagggttt tatggggaaa
     2701 ggccattgga atcggaaaag agtttatagc attcgtaagt ttgctgtggg agcttgctca
     2761 gtaatgattg ggacttgtgc agttctatta ggaggaaata tagctggaga atctgtagtt
     2821 tatgcggatg aaacacttat tactcatact gctgagaaac ctaaagagga aaaaatgata
     2881 gtagaagaaa aggctgataa agctttggaa actaaaaatg tagttgaaag gacagaacaa
     2941 agtgaaccta gttcaactga ggctattgca tctgagaaga aagaagatga agccgtaact
     3001 ccaaaagagg aaaaagtgtc tgctaaaccg gaagaaaaag ctccaaggat agaatcacaa
     3061 gcttcaagtc aagaaaaacc gctcaaggaa gatgctaaag ctgtaacaaa tgaagaaatg
     3121 aatcaaatga ttgaagacag gaaagtggat tttaatcaaa attggcactt taaactcaat
     3181 gcaaattcta aggaagccat taaacctgat gcagacgtat ctacgtggaa aaaattagat
     3241 ttaccgtatg actggagtat ctttaacaat ttcgatcatg aatctcctgc acaaaatgaa
     3301 ggtggacaac tcaacggtgg ggaagcttgg tatcgcaaga ctttcaaact agatgaaaaa
     3361 gacctcaaga aaaatgttcg ccttactttt gatggcgtct acatggattc tcaagtttat
     3421 gtcaatggtc agttagtggg gcattatcca aatggttata accagttctc atatgatatc
     3481 accaaatacc ttcacaaaga tggtcgtgag aatgtgattg ctgtccatgc agtcaacaaa
     3541 cagccaagta gccgttggta ttcaggaagt ggtatctatc gtgatgtgac tttacaagtg
     3601 acagataagg tgcatgttga gaaaaatggg acaactattt taacaccaaa acttgaagaa
     3661 caacaacatg gcaaggttga aactcatgtg accagcaaaa tcgtcaatac ggacgacaaa
     3721 gaccatgaac ttgtagccga atatcaaatc gttgaacgag gtggtcatgc tgtaacagac
     3781 ttagttcgta cagcgagtcg taccttaaaa gcacatgaat caacaagcct agatgcgatt
     3841 ttagaagttg aaagaccaaa actctggacc gttttaaatg acaaacctgc cttgtacgaa
     3901 ttgattacgc gtgtttaccg tgacggtcaa ttggttgatg ctaagaagga tttgtttggt
     3961 taccgttact atcactggac tccaaatgaa ggtttctctt tgaatggtga acgtattaaa
     4021 ttccatggag tatccttgca ccacgaccat ggggcgcttg gagcagaaga aaactataaa
     4081 gcagaatatc gccgtctcaa acaaatgaag gagatgggag ttaactctat ccgtacaacc
     4141 cacaaccctg ctagtgagca aaccttgcaa atcgcagcag aactaggttt actcgttcag
     4201 gaagaggcct ttgatacgtg gtatggtggc aagaaacctt atgactatgg acgtttcttt
     4261 gaaaaagatg ccactcaccc agaagctcga aaaggtgaaa aatggtctga ttttgaccta
     4321 cgtaccatgg tcgaaagagg caaaaacaac cctgctatct tcatgtggtc aattggtaat
     4381 gaaataggtg aagctaatgg tgatgcccac tctttagcaa ctgttaaacg tttggtcaag
     4441 gttatcaagg atgttgataa gactcgctat gttaccatgg gagcagataa attccgtttc
     4501 ggcaatggta gcggagggca tgagaaaatt gctgatgaac tcgatgctgt tggatttaac
     4561 tattctgaag ataattacaa agcccttaga gctaagcatc caaaatggtt gatttacggt
     4621 tcagaaacat catcagcaac ccgtacacga ggaagttact atcgccctga acgtgaattg
     4681 aaacatagca atggacctga gcgtaattat gaacagtcag attatggaaa tgatcgtgtg
     4741 ggttggggga aaacagcaac cgcttcatgg acttttgacc gtgacaacgc tggctatgct
     4801 ggacagttta tctggacagg tacggactat attggtgaac ctacaccatg gcacaaccaa
     4861 aatcaaactc ctgttaagag ctcttacttt ggtatcgtag atacagccgg cattccaaaa
     4921 catgacttct atctctacca aagccaatgg gtttctgtta agaagaaacc gatggtacac
     4981 cttcttcctc actggaactg ggaaaacaaa gaattagcat ccaaagtagc tgactcagaa
     5041 ggtaagattc cagttcgtgc ttattcgaat gcttctagtg tagaattgtt cttgaatgga
     5101 aaatctcttg gtcttaagac tttcaataaa aaacaaacca gcgatgggcg gacttaccaa
     5161 gaaggtgcaa atgctaatga actttatctt gaatggaaag ttgcctatca accaggtacc
     5221 ttggaagcaa ttgctcgtga tgaatctggc aaggaaattg ctcgagataa gattacgact
     5281 gctggtaagc cagcggcagt tcgtcttatt aaggaagacc atgcgattgc agcagatgga
     5341 aaagacttga cttacatcta ctatgaaatt gttgacagcc aggggaatgt ggttccaact
     5401 gctaataatc tggttcgctt ccaattgcat ggccaaggtc aactggtcgg tgtagataac
     5461 ggagaacaag ccagccgtga acgctataag gcgcaagcag atggttcttg gattcgtaaa
     5521 gcatttaatg gtaaaggtgt tgccattgtc aaatcaactg aacaagcagg gaaattcacc
     5581 cttactgctc actctgatct cttgaaatct agtcaagtca ctgtctttac tggtaagaaa
     5641 gaaggacaag aaaagactgt tttggggaca gaggtgccaa aagtacagac cattattgga
     5701 gaggcacctg aaatgcctac cactgttccg tttctataca gtgatggtag tcgtgcagaa
     5761 cgtcctgtaa cctggtcttc agtagatgtg agcaagcctg gtattgtaac ggtgaaaggt
     5821 atggctgacg gacgagaagt agaagctcgt gtagaagtga ttgctcttaa atcagagcta
     5881 ccagttgtga aacgtattgc tccaaatact gacttgaatt ctgtagacaa atctgtttcc
     5941 tatgttttga ctgatggaag tgtacaagag tacgaagtag atagctggga gattacggaa
     6001 gtagataaag ccaaactttc agtagctgga tcacgtattc aaatgactgg tcagttagct
     6061 ggagaaacta ttcatgcaac ccttgtggta gaagaaggaa atgctgcagc acctgtagtg
     6121 ccaactgtta ctgttggagg tgaagcagta acaggtctta ctagtcgaca accaatgcaa
     6181 tatcgtactc tatcttatgg tgcccaattg ccagaagtca cagcaagtgc tgaaaatgct
     6241 gatgtgacag ttcttcaagc aagcgcagca aacggcatgc gtgcaagcat atttgttcag
     6301 cctaaagatg gtggccctct tcaaacctat gcaattcaat tcctagaaga agcaccaaaa
     6361 attgctcact tgagcttgca agtggaaaaa gctgacagtc tcaaagaaga ccaaactgtc
     6421 aaattgtcgg ttcgagctca ctatcaagat ggaacgcaag ctgtattacc agctgataaa
     6481 gtaaccttct ctacaagtgg tgaaggggaa gtcgcaattc gtaaaggaat gcttgagttg
     6541 cataagccag gagcagtcac tctcaaagcg gaatatgagg gagctaaagg ccaagttgaa
     6601 ctcactatcc aagccaatac tgagaagaag attgcgcaat ctatccgtcc tgtaaatgta
     6661 gtcacagatt tgcatcaaaa acctactctt ccaacaacag taacggttga gtatgacaaa
     6721 ggtttcccta aagctcacaa agtcacttgg caagctattc cgaaagaaaa actagactcc
     6781 tatcaaacat ttgaagtact aggtaaagtt gaaggaattg accttgaagc gcgtgcaaaa
     6841 gtctctgtag aaggtatcgt ttcagttgaa gaagtcagtg tgacaactcc aatcgcagaa
     6901 gcaccacaat taccagaaag tgttcggaca tatgattcaa atggtcacgt ttcatcagct
     6961 aaggttgcat gggatgcgat tcgtccagag caatacgcta aggaaggtgt ctttacagtt
     7021 aatggtcgct tagaaggtac tcaattaaca actaaacttc atgttcgcgt atctgctcaa
     7081 actgagcaag gtgcaaacat ttctgaccaa tggaccggtt cagaattgcc acttgccttt
     7141 gcttcagact caaatccaag cgacccagtt tcaaatgtta atgacaagct catttcctac
     7201 aataaccaac cagccaatcg ttggacaaac tggaatcgta ctaatccaga agcttcagtc
     7261 ggtgtcctat tcggagattc aggtattttg agcaaacgtt cagttgataa cttaagcgtt
     7321 ggcttccatg aagaccatgg agttggtgca ccgaagtctt atgtgattga gtattatgtt
     7381 ggtaagactg tcccaacagc tcctaaaaac cctagttttg ttggtaatga ggaccatgtc
     7441 tttaatgatt ctgccaactg gaaaccagtt actaatctaa aagcccctgc tcaactcaag
     7501 gctggagaaa tgaaccactt tagctttgat aaagttgaaa cctatgctgt tcgtattcgc
     7561 atggtgagag cagacaacaa actaggaacg tctatcacag aggtacaaat ctttgcgaaa
     7621 caagttgcgg cagccaagca aggacaaaca agaatccaag ttgacggtaa agacttagca
     7681 aacttcaacc ctgatttgac agactactac cttgagtctg tagatggaaa agttacggca
     7741 gtcacagcaa gtgttagcaa caatggcctc gctaccgtcg ttccaagcgt tcgtgaaggt
     7801 gagccggttc gtgtcatcgc gaaagctgaa aatggcgaca tcttaggaga ataccgtctg
     7861 cacttcacta aggataagaa cttactttct cataaaccag ttgctgcggt taaacaagct
     7921 cgcttgctac aagtaggtca agcacttgaa ttgccgacta aggttccagt ttacttcaca
     7981 ggtaaagacg gctacgaaac aaaaaacctg acagttgaat gggaagaagt tccagcggaa
     8041 aatctgataa aagcaggtca atttaccgtt cgaggccatg tccttggtag tgatcttgtt
     8101 gctgaggtca ctgtacgagt gacagacaaa ctaggcgaag ctctttcaga taaccctaac
     8161 tatgatgaaa acagtaacca ggcctttgct tcagcaacca atgatattga caaaaactct
     8221 catgaccgcg ttgactatct caatgacgga gatcattcag aaaatcgtcg ttggacaaac
     8281 tggtctccaa caccatcttc taatccagaa gtatcagcgg gtgtgatctt ccgtgaaaat
     8341 ggtaagattg tagaacggac tgttgctcaa gccaaacttc acttctttgc agatagtggt
     8401 acggatgcac caactaaact cgttttagaa cgctatgtcg gtccagagtt tgaagtgcca
     8461 acctactatt caaactacca agcctacgac gcagaccatc cattcaacaa tccagaaaat
     8521 tgggaagctg tgccttatcg tgcggataaa gacatcgcag ctggtgatga aatcaacgta
     8581 acatttaaag ctgtcaaagc caaagctatg agatggcgta tggagcgtaa agcagataag
     8641 agcggtgttg cgatgattga gatgaccttc cttgcaccaa gtgaattgcc tcaagaaagc
     8701 acgcaatcga agattcttgt agatggaaaa gaacttgctg atttcgctga aaatcgtcaa
     8761 gactatcaaa ttacctataa aggtcaacgg ccaaaagtct cagttgaaga aaacaatcaa
     8821 gtagcttcaa ctgtggtaga tagtggagaa gatagccttc cagtacttgt tcgcctcgtt
     8881 tcagaaagtg gaaaacaagt caaggaatac cgtatccagt tgactaagga aaaaccagtt
     8941 tctgagaaga cagttgctgc tgtacaagaa gatcttccaa aactcgaatt tgttgaaaaa
     9001 gatttggcct acaagacagt tgagaaaaaa gattcaacac tgtatctagg tgaaactcgt
     9061 gtagaacaag aaggaaaaac tggtaaagaa cgtatcttta cagcgattaa tcctgatgga
     9121 agtaaggaag aaaaactccg tgaagtggta gaagctccga cagaccgcat cgtcttggtt
     9181 ggaaccaaac cagtagctca agaagctaaa aaaccacaag tgtcagaaaa agcagataca
     9241 aaaccaattg attcaagtga agctagtcaa actaataaag cccagttacc aaatacaggt
     9301 agtgcggcaa gccaagcagc agtagcagca ggtttagctc ttctaggttt gagtgcagga
     9361 ttagtagtta ctaaaggtaa aaaagaagac tagaaatttt gataccttct ttatcattaa
     9421 agaccaaaga aaaactcttg tcctaaatag aagataaaga gtctggaaca aaatagttct
     9481 caaccacaaa aaagctagat atttccaatt gcggaactct agctttttaa ttttgagtcg
     9541 ttctcttatt gtatagtaga ttgaaactag agtagtacac ttctacttct aaaacattgt
     9601 tagaaatcga tttgactgtc cttatcgatt tgtcctgttc ttatttcatt tcactatgtt
     9661 ttaggaggtt attggccata agtaccaatc ccatgtcaat tctcacttga cgcttgcctc
     9721 tcagattaca tctcttgtaa cccaaacaag cctttatctg cccaaagaca ggttctacat
     9781 caatcttgcg ttgagcgaaa atctgtctat cttggggaga taaaagcgcc tgacattctt
     9841 tagctttcaa gttttgatag cgttcgttca tatacaatcc cttttgaggg gctgattcag
     9901 gttcatcagc gtagtaaacc ttgatttcct gttgaaagtc tgtctgtgtt ttctggtact
     9961 tgatatggtg aaaacgatag caccagccat caggatgtat gtagctatcc tccttgtcat
    10021 tatagtgcca attcgctaag tttttagctg actgtttata ccctctcttt tgttccttat
    10081 caaacatggc atatttaatc agaaggggag tgatttcttc ttgaaaatag acttgtatct
    10141 gttcttgaag tttggcggag aatttctctg tcgttttctt ccacacaaaa ctatacttgt
    10201 tggcattgga ttcaatctta gttccgtcaa taaacaaaca atctaaggtc actaactctt
    10261 ccatttttca atttttcgac cagagaaaat cccttgtgaa taggcaaata gaagagtaga
    10321 tacaagcatt ttagggtgat aagacgggcg accaaaggca tgataggagg tgtagaagtg
    10381 acgttcctcc aaggtattca ccactttttc aatagtaaag acgagatggt cttgtggtaa
    10441 gaaggaactg atttctagtg gtaaagttgt ttgatttgtg ttatagtgaa tatgcatggg
    10501 atagcctttc taaatggttt attaagcaat tctattttac caagactatc gtaaccatgc
    10561 aaaaaagcaa cggcaaatcc gttgcctttt ttggatataa gagactattg tcccagactc
    10621 ttttctttgt ataaaattgt atagtgttat tgtctatact attttgtaga gaaagcaaaa
    10681 atccctagaa aatgcttagg gattcgacta tttcattaag aacaattcaa caatcatctc
    10741 tatatacatg ataagggtta ataaaaatcc tgtacgccag aagaatttga tgaatttagg
    10801 atagtaaaag ctgcgttttt ttaaaaggaa gaaaaagacg agaataatgg ctaggaacga
    10861 gagggctagc cccagtctag ggaggaaatt atgggtaaag gttttagctg taatcaagta
    10921 atactcaaat accaagactg gaaaagccaa atccgcaaag tttcgtccta gtttttttaa
    10981 tctaaaaagt ttggttagga taatgcagac gactaaggtc agtatcaata ataaaataga
    11041 tgctaatttc attaaaatca tacccatatt gtatcataaa aaaaggctaa tggaaacgag
    11101 aaacagagga atttatagga aagtcaggct tttgagattg tgggtgattt ttgttataat
    11161 gaaagttata aaatcttatg ataccttgaa tgattattat cgaaaactct ttggagaaaa
    11221 gacttttaaa gtccctattg atgcgggatt tgactgtccc aatcgtgatg gaactgtggc
    11281 tcatggaggc tgtacttttt gtacggtttc cggttctgga gatgccattg tagccccaga
    11341 tccgcctatc cgtgagcaat tttataagga aattgacttt attcatcgta agtggccaga
    11401 tgttcagaag tatctggttt attttcaaaa ttttaccaac acccatgaaa aggtggaagt
    11461 tattcgagag cgttatgaac aggctatcaa tgagccaggt gtggtaggaa tcaatattgg
    11521 aacgcgccca gactgtttac cagacgaaac catcgaatat ttggctgagt tatcggagtg
    11581 catgcatgtg acggttgaat tgggcttgca gactacttat gaagcaacct ctgacctgat
    11641 taaccgtgcg cattcttatg aattgtaggt ggaaacggtc aagcgtttga gaaagtatcc
    11701 aaaaattgag attgtttccc atttgatcaa tggtttgcct ggtgaaaccc atgagatgat
    11761 ggttgaaaat gtccgccgtt gtgtcacgga taacgatatt caagggatta aactgcattt
    11821 gctccatctt atgacaaata ctcgtatgca acgagattac catgagggac gtttacaatc
    11881 gatgagtcag gacgaatatg tcagggtcat ctgtgaccaa ctggaaatta ttcccaaaca
    11941 tatcgtcatc catcgaatta caggagatgc gcctagggat atgctgattg gtcctatgtg
    12001 gagcctcaat aaatgggaag tcctcaacag cattgagatg gagatgagac gtcgtggaag
    12061 tgttcaagga tgcaaggctg taaaacagga gtttgaaaat gaaaagacca cttgagatgg
    12121 cacatgattt tttggctgag gtcgtgacaa aagaggatgt cgtagtggat gcgactatgg
    12181 gaaatggtca tgacacgctt tttttagcca agctagccaa gcaagtctat gcctttgata
    12241 ttcagaagca agccttggaa aagacccaag agcgtttgca tcaggctggc ttgacaaatg
    12301 cccagttaat cttgcaaggc catgagacac tggaccagtt tgtgacaaaa gctaaggcag
    12361 ggatttttaa tttgggctat ttgccgtcag ctgataagtc tgtcatcacc cgaccgcaga
    12421 caacgattga ggcattagaa aagctatgtg gcttacttgt caaaggtgga cgaattgcta
    12481 ttatgattta ctatggtcat gaaggaggcg acctcgagag agatgctgtc ttggattttg
    12541 tgatccagtt gaaccaacaa gagtacacag ctgccattta ccgaacttta aaccaagtca
    12601 acaacccgcc gtttttagtg atgattgaaa aattagagag atacagacat ggataaacaa
    12661 tacctacacg aaaagctgga tgccatgcgc cagaattttg ttgaatcaac ctaccacgaa
    12721 cgagcggtgg gcgtgctaga ccaagcgcat atgagcaaaa aaatgcttaa aatcaagaaa
    12781 aaattagttg ctcttgaaat ggaacggtgc cagagaaaaa ttgagcacaa agactgttcc
    12841 aagattgacc aaaaaatcaa agagcagaag gagatatttg aatcctgttg taaaaaagat
    12901 taaggaggac gtgcgtggaa ttactgattt acctcatcct atttttactg gtcttgattg
    12961 tctcaagtac aaccaataag ctcctaccct ttttgcctct ccctttggtg cagattcttt
    13021 tgggaattgt gattggtctc tttttaccca atactgactt tcaccttaat acggagttgt
    13081 ttttggcact ggttatcggc cccttgcttt tccgagaggc tgaagaagca gatgttacgg
    13141 ctattttaaa acactggcga atcattgttt atctcatatt tccagtgatt tttatctcga
    13201 ccctgagttt gggtggcttg gcccatcttc tttggttcag ccttcccttg gcagcttgct
    13261 tggctgttgg ggcagccctt ggtcctacgg acttggtggc ctttgcctct ctttcggagc
    13321 gttttagctt tcctaagcgc gtgtccaata ttcttaaggg cgaaggactc ttgaatgatg
    13381 cttctggttt ggtggctttt caggtagctt tgacagcttg gacaactgga gctttttctc
    13441 tggggcaagc tagcagttca ctcatctttt caatcctagg cggtttttta attggatttt
    13501 taacagccat gaccaaccgc ttcctccata ccttcttgct aagtgtgcgt gcaacggata
    13561 ttgccagtga acttttatta gaattgagtt tgcctcttgt gaccttcttt ctggcagaag
    13621 aagttcatgt ttcaggtatt attgccgtcg tagttgctgg aattttaaag gcaagtcgtt
    13681 tcaagaaaat cacgctcctc gaagcccaag tggatacggt gaccgagacg gtctggcata
    13741 cagtgacctt tatgctcaac ggttctgtct ttgtgatttt agggatggag ctggaaatga
    13801 tagcagaacc tatcttgacc aatccaatct ataatcctct acttttattg ctatctctta
    13861 tcgcccttac ctttgtcctc tttgtcattc gttttattat gatctatggc tattatgcct
    13921 atagaacccg acgcctcaag aaaaagctaa ataagtatat gaaggacatg tttctcttga
    13981 ccttttcagg tgttaaggga acggtgtcga ttgctacgat tctcttgata ccaagtaatc
    14041 tagaacagga gtatcctctc ttgcttttcc ttgttgcagg tgtgacgctt gttagctttt
    14101 taacaggtct cttggtcttg cctcatcttt ctgatgaaga ggaagaaagc aaggattatc
    14161 tcatgcatat cgccattttg aatgaagtaa cgctagagtt ggaaaaagag ttggaagaca
    14221 ccagaaataa actttccctc tatgcggcta ttgacaatta tcatggacgt attgaaaatc
    14281 tcattttaag ccaagaaaac caggatgatc aagaagactg ggctgctttg aaactcttga
    14341 ttcttagtat tgaaagtgat ggtttggaac aggcctatga agaggggaat attagcaatc
    14401 gtgcttaccg agtttaccaa cgttatctga aaaatataga acaaggaatc aatcgtaaac
    14461 ttgcctcaag actgacctat tattttcttg tttccttgag gattttacgt tttctccttc
    14521 atgaagtttt tactcttgga aagaccttcc gtagctggaa ggacaaggag caaagccgtc
    14581 tccgtgctct tgattatgac caaattgcag agctctatct tgccaataca gagatgatta
    14641 ttgaaagttt ggaaaacctg aagggagtct acagacgctc tttgattagt tttatgcagg
    14701 agtctcgtct tcgagaaaca gctattatca gcagtggtgc ctttgtcgaa cgggttatca
    14761 atcgtgtcaa acccaacaat atcgatgaaa tgctgagagg ctattatctg gagcgcaagt
    14821 tgattttcga atacgaagaa aaacgattaa ttacaactaa gtatgccaag aaattacgac
    14881 aaaatgtaaa taacttagag aactattcct tgaaggaagc tgccaatacc ctgccgtatg
    14941 atatggtgga attggtaaga agaaattagt taatactctt cgaaaatctc ttcaaaccac
    15001 gtcagcgtcg ccttggatta tatatgtgac tgacttcgtc agtttcatct acaacctcaa
    15061 agcagggctt tgagcaacct gcggctagct tcctagtttg ctctttgatt ttcattgagt
    15121 ataagattat aaatgaagga gtatgacatg aaaaagtggt ggaaagagct gatagacaag
    15181 cctttattaa aagctttttt gcattattat caagcatcag atagtgagtt gaccagtgtc
    15241 gcagtagcct actattggtt gatttcgatt ttccccctgc ttttggtggt ggtcaatatc
    15301 ctcccctatt ttcagattcc tgtgggagaa tttctgtgct ttatgaaaga cgttctgccc
    15361 ccaagcctct atgaaggtgt ggaaaaaata gccagagaag ttttgacgca accttcaaca
    15421 ggtttattga gtttttctgt tttatcggcg ttatggagtt tttcaaaatc tatgaatttc
    15481 ttgcaaaaag cttttaacaa ggcttatggc gtcgaaaaga gtcgtggact tatttcccat
    15541 cagatgctaa gtctcctagt cagttttggc ttgcagctcc tctttgcgtt tgccttgttt
    15601 ttgatcttat ttggtcagat gattttggct ttacttgctc attattggac aaaagatggc
    15661 attatctatc aggctttaca agggttggca ggtcctctga tttacgccct cctctttgct
    15721 atcttggtca tgctttatta ttttcttccc aatatatcta acaggaaaat tagctatacc
    15781 ctaccaggca gtgcctttgt tctcttagtg attttaggtt tactaactct gttctctagt
    15841 tatctcaatt actatgtcca tcatttggtg gatgtccgaa ttttaggttc cgttcttctt
    15901 gttgttatga tgttttggtt tatcttgatt gctaaaattg ttatcctagg cgcggttatc
    15961 aatgccagta tgcagagttt gagagatccg gtctttaaaa aagactaact atttatccat
    16021 tacaaaagcg catagtatca aaactttata gaaccttgat actatgcgct tttgtgcttt
    16081 gacagttttt tgtatagttt ttgtccaaga tttctaaatc atctgattaa attcctattt
    16141 tcccctatca ttgtccctgt tttttcgaaa atggaaggag tataataaat ctatcaataa
    16201 aatgatagga ggaactttat gggtcatatt ttcttttttc taagtgtctt tttggcaggg
    16261 attctatcct tcttttctcc ttgtatctta cctttgttac cggtctatac aggagtgtta
    16321 ctagatgata aggatggtgc tcaggcttct agcggcaaat tttcaatctc agttactagt
    16381 ttattacgaa ctctggcctt tatagcagga atttccttta tatttatttt gttgggctat
    16441 ggagctggtt ttttaggcga tttgctttat gcttcttggt tccaatatct tactggggca
    16501 attattatcc ttcttggttt gcaccaaatg gagattctac actttaaggg gctttataag
    16561 gaaaagaggc tacaactgca aggacagggg caaaatggta agggctatag tcaggcattt
    16621 ttattgggct tgacctttag ttttgcttgg acgccttgcg tggggccggt tctggggtct
    16681 gttttggcct tggcggcttc aggtggttca ggagcttggc agggagctgg tctcatgttg
    16741 gtgtatacgc tgggcttggc gctaccattc ttgcttctag ctctgacctc tagttatgtt
    16801 ttgaaacatt tccgaaaact tcatccctat ctcggaatcc tcaaaaaagt gggtggtttt
    16861 ctcattattg tgatgggact cttggttctg tttggaaatg cttcaatttt aagtcaatta
    16921 tttgaataaa atggaaagga atatcaatat gaaaaaatgg caaacatgtg ttcttggagc
    16981 aggttcgctc ctttgtttga cggcttgttc aggcaagtcc gtgactagtg aacaccaaac
    17041 gaaagatgaa atgaagacgg agcagacagc tagtaaaaca agcgcagcta aagggaaaga
    17101 ggtggctgat tttgaattga tgggagtaga tggcaagacc taccgtttat ctgattacaa
    17161 gggcaagaaa gtctatctca aattctgggc ttcttggtgt tccatctgtc tggctagtct
    17221 tccagatacg gatgagattg ctaaagaagc tggtgatgac tatgtggtct tgacagtagt
    17281 gtcaccagga cataagggag agcaatctga agcggacttt aagaattggt ataagggatt
    17341 ggattataaa aatctcccag tcctagttga cccatcaggc aaacttttgg aaacttatgg
    17401 tgtccgttct tacccaaccc aagcctttat agacaaagaa ggcaagctgg tcaaaacaca
    17461 tccaggattc atggaaaaag atgcaatttt gcaaactttg aaggaattag cctaggaggt
    17521 gtcttatgaa tgataagtta aaaatcttct tgttgctagg agtatttttt ctagccataa
    17581 ccggtttcta tgttctattg atacgaaatg cagggcagac agatgcctcg caaattgaaa
    17641 aggcggcagt tagccaagga ggaaaagcag tgaaaaaaac agaaattagt gaagacgcag
    17701 acttgcacga aatttatcta gctggaggtt gtttctgggg agtggaggaa tatttctcac
    17761 gcgttcccgg ggtgacggat gccgtttcag gctatgcaaa tggtagagga gaaacaacca
    17821 agtacgaatt aattaaccaa acaggtcatg cagaaaccgt ccatgtcacc tatgatgcca
    17881 agcaaatttc tctcaaggaa atcctgcttc actatttccg cattatcaat ccaaccagca
    17941 aaaataaaca aggaaatgat gtggggaccc agtaccgtac tggtgtttat tacacagatg
    18001 acaaggattt ggaagtgatt aaccaagtct ttgatgaggt ggctaagaaa tacgatcaac
    18061 ctctagcagt tgaaaaggaa aacttgaaga attttgtggt ggctgaggat taccatcaag
    18121 actatctcaa gaaaaatcca aatggttact gccatatcaa tgttaatcag gcggcctatc
    18181 ctgtcattga tgccagcaaa tatccaaaac caagtgatga ggaattgaaa aagaccctgt
    18241 cacctgagga gtatgcagtt acccaggaaa atcaaacaga acgagctttc tcaaaccgtt
    18301 actgggataa atttgaatcc ggtatctatg tggatatagc aactggggaa cctctctttt
    18361 catcaaaaga caaatttgag tctggttgtg gctggcctag ttttacccaa cccatcagtc
    18421 cagatgttgt cacctacaag gaagataagt cctacaatat gacgcgtatg gaagtgcgga
    18481 gccgagtagg agattctcac cttgggcatg tctttacgga tggtccacag gacaagggcg
    18541 gcttacgtta ctgtatcaat agcctctcta tccgctttat tcccaaagac caaatggaag
    18601 aaaaaggcta cgcttattta ctagattatg ttgattaata ctcttcgaaa atctcttcaa
    18661 accacgtcag cgtcgcctta ccgtatgtag ggttactgac ttcgtcagtt ttatctacaa
    18721 cctcaaagct atactttgag ctgacttcgt cagttttatc tacaacctca aagcggtact
    18781 ttgagaaacc tgtggctatc ttcctagttt actttttgat ttttattgag tataagaggt
    18841 ctttcctaag cagttagagg agaattttgc tatactgata ctagtaagtg gtaaaggagc
    18901 agagcatgac ctacacaatc ttaatcgtag aagatgaata tctggtaaga caaggtttga
    18961 ctaaactggt caatgtagca gcctacgata tggaaatcat cggtcaggct gaaaatggaa
    19021 ggcaggcttg ggaattgatc caaaagcagg tgccagatat cattttaacc gatatcaaca
    19081 tgcctcatct aaatggcatc cagttggcca gtctggtacg agaaacctat cctcaggttc
    19141 atttggtctt tttaacaggt tacgatgatt ttgattatgc cttgtctgct gtcaaactag
    19201 gtgtggacga ctacctgctc aaaccctttt ctcgtcagga tattgaggaa atgttgggga
    19261 aaatcaaaca aaaactagac aaggaagaga aagaagagca gttacaagat ttattgacta
    19321 ataggtttga aggaaacatg gcccagaaaa tccagtctca tctggctgat agccaattta
    19381 gtttaaagtc tttagccagt gacttaggtt ttagtccgac ctatctgagt tccttgatta
    19441 agaaagagtt gggcttgcct tttcaggatt atctggtgag agaacgtgtt aaacaagcca
    19501 agctcttgct tttaactaca gatctgaaga tttatgagat cgcagagaag gttggttttg
    19561 aagatatgaa ctattttacc caacgtttta agcagattgc aggtgtgaca cctcgtcagt
    19621 ttaagaaggg agaagaccga tgaagcgttc ttctctttta gttagaatgg ttatttccat
    19681 ctttctggtc tttctcattc tcctagctct ggttggaact ttctactatc aatcaagttc
    19741 ttcagccatt gaggccacca ttgagggcaa cagccaaacg accatcagcc agactagcca
    19801 ctttattcag tcttatatca aaaaactaga aaccacctcg actggtttga cccagcagac
    19861 ggatgttctg gcctatgctg agaatcccag tcaagacaag gtcgagggaa tccgagattt
    19921 gtttttgacc atcttgaagt cagataagga cttgaaaact gttgtgctgg tgaccaaatc
    19981 tggtcaggtc atttctacag atgacagtgt gcagatgaaa acttcctctg atatgatggc
    20041 tgaggattgg taccaaaagg ccattcatca gggagctatg cctgttttga ctccagctcg
    20101 taaatcagat agtcagtggg tcatttctgt cactcaagaa cttgttgatg caaagggagc
    20161 caatcttggt gtgcttcgtt tggatatttc ttatgaaact ctggaagcct atctcaatca
    20221 actccagttg gggcagcagg gctttgcctt cattatcaat gaaaaccatg aatttgtcta
    20281 ccatcctcaa cacacagttt atagttcgtc tagcaaaatg gaggctatga aaccctacat
    20341 cgatacaggt cagggttata ctcctggtca caaatcctac gtcagtcaag agaagattgc
    20401 aggaactgat tggacggtgc ttggcgtgtc atcattggaa aagttagacc aggttcggag
    20461 tcagctcttg tggaccttgc ttggggccag tgtcacatct cttcttgtct gtctctgctt
    20521 agtgtggttc agtcttaaac gctggattgc tcctttgaag gatttgagag aaaccatgtt
    20581 ggaaattgct tctggtgctc aaaatcttcg tgccaaggaa gttggtgcct atgaactgag
    20641 agaagtaact cgccaattta atgctatgtt ggatcagatt gatcagttga tggtagctat
    20701 tcgtagccag gaagaaacga cccgtcagta ccaacttcaa gccctttcga gccagattaa
    20761 tccacatttc ctctataaca ctttggacac catcatctgg atggctgaat ttcatgatag
    20821 tcagcgagtg gtgcaggtga ccaagtcctt ggcaacctat ttccgcttgg cgctcaatca
    20881 aggcaaggac ttgatttgtc tctctgacga aatcaatcat gtccgccagt atctctttat
    20941 ccagaaacaa cgctatggag ataagctgga atacgaaatt aatgaaaatg ttgcctttga
    21001 taatttagtc ttacccaagc tggtcctaca accccttgta gaaaatgctc tttaccatgg
    21061 cattaaggaa aaggaaggtc agggccatat taaactttct gtccagaaac aggattcggg
    21121 attggtcatc cgtattgagg atgatggcgt tggcttccaa gatgctggtg atagtagtca
    21181 aagtcaactc aaacgtgggg gagttggtct tcaaaatgtc gatcaacggc tcaaacttca
    21241 ttttggagcc aattaccata tgaagattga ttctagaccc caaaaaggga cgaaagttga
    21301 aatatatata aatagaatag aaactagcta actcccagtc aattctggga gttttatgcg
    21361 taattgggca aactttctag gttgtctatc ttgctaaaaa caagaaaaaa cggtatgata
    21421 gaagagtgac aacagaggta acagagatga acgagacaca gattcaaaga gaaacacgtc
    21481 aggtagtgga agatgtttta gaaaagacca atttgaagca gggggccctt tttgttttag
    21541 gtctatcttc tagtgaggtg ctaggtggtc atattgggaa agaatccagt caagagattg
    21601 gtgagctgat agtggagact atcttggata ttctaggtag tagaggtatc catttagcag
    21661 ttcaaggttg tgagcatgtc aatcgggctc tcgttgttga acgtcaggtg gcagagcagt
    21721 ttggtctgga aattgtcagt gtccatccta ctcttcatgc aggaggttcg gggcagttgg
    21781 cagcctttaa gtttatgcag gatccagttg aggttgaatt tatcaaggct catgctggat
    21841 tggatatcgg agacactgca attggcatgc atgtcaagca tgttcaggtt ccgattcgcc
    21901 ctattttgag agagattggt catgcccatg taacggcact ggctagtcgt ccaaaattaa
    21961 tcggaggtgc gcgtgcgcac tatccgcaag atgctattag aaagacctga gaatgaataa
    22021 aggagataaa agtgtttaaa aaagaccgtt tttcaattcg taagattaag ggagttgtag
    22081 gctctgtatt tcttggaagc cttttgatgg ctccttctgt agtggacgca gccacctatc
    22141 actatgtaaa taaagagatt atttcacaag aagctaaaga tttaattcag acaggaaagc
    22201 ctgacaggaa tgaagttgta tatggtttgg tgtatcaaaa agatcagttg cctcaaacag
    22261 ggacagaagc atctgttttg acagcttttg gttatctatc aggtgacatc ctcaaaacgc
    22321 ttggcttgga cactgtttta gaagaaacct cagcaaaacc tggagaggtg actgtagtcg
    22381 aagttgagac tcctcaatca acaacaaatc aggagcaagc taggacagaa aaccaagtag
    22441 tagagacaga ggaagctcta aaagaagaag cacctaaaac agaagaaagt ccaaaggaag
    22501 aaccaaaatc ggagataaaa cctactgacg acacccttcc taaagtagaa gaggggaaag
    22561 aagattcagc agaaccagct ccagttgaag aagtaggtgg agaagttgag tcaaaaccag
    22621 aggaaaaagt agcagttaag ccagaaagtc aaccatcaga caaaccaaca gaggaaccaa
    22681 aagttgaaca agtaggtgaa ccagtcgagc caagtgaaga cgaacaggca ccaacggcac
    22741 cagttgagcc agaaaagcaa ccagaagctc ctgaagaaga gaaggctgta gaggaaacac
    22801 cgaaaccaga agataaaata aagggtattg gtactaaaga accagttgat aaaagtgagt
    22861 taaataatca aattgataaa gctagttcag tttctcctac tgattattct acagcaagtt
    22921 acaatgatct tggacctgtt ttagaaactg caaaaggtgt ctatgcttca gagcctgtaa
    22981 aacagcctga ggtaaatagt gagacaaata aacttaaaac ggctattgac gctctaaacg
    23041 ttgataaaac tgaattaaac aatacgattg cagatgcaaa aacaaaggta aaagaacatt
    23101 acagtgatag aagttggcaa aacctccaaa ctggggttac agaagctgaa aaagttgcag
    23161 ctaatacaga tgctaaacaa agtgaagtaa attctgagac tgcaagtctt aaaacagcaa
    23221 tttctagatt aaatacagat aaagttgaac tcgaaaatca acttaagatt gcacagggaa
    23281 aaactgaaac tgatttcagc atggaatctt ggactgtttt gagtacagct aaaaataaag
    23341 ctcaggaagt aaaagataac ggaacagcga cccaagaaca aataaacgaa gcagagaaaa
    23401 gtttgaaaac agcccttgct gacttatctg ttgacaaaac agctttggga agcgctatcg
    23461 atacagctac taagaaaaat aaagagaatt atacaaatca aacatgggca gagttggaaa
    23521 cagcgcttac agcagcaaaa tctgtaaata ctaacgagtc taaacaaagc gacgttaacg
    23581 aagctgctga aaaattaact gcaactatgg aaaaattggt tgaattgtct gaaaagccac
    23641 gtttgactct atctattgaa aagagagaca tagatcgaaa agctacggtc acgtatactt
    23701 tggaaaatcc agcaaatact caaataaaat caataactgc aactctgaaa aaaggagagg
    23761 aagttgttaa ggattttgtt ttaacagaag aaaatcttaa aactaatcat ttaactgctt
    23821 tgtttgaaaa gctagactac tacaaagaat acactctatc tacggatatg gtatataaca
    23881 gaggaaatga tgatgagacg gagagtattt cagaagaact aattcagctg aatcttaaga
    23941 aactagaatt gaaagatatc cagacagtct ctctgatgaa atttgaaaat ggtcaagaaa
    24001 gtcaggttac acacttgtct gataaaccga ccgatttgag taaattatac ttaaaagtta
    24061 catcatcaac gtctaaggat gctgtgttag ccgttagctc tatagaagaa gagattattg
    24121 aaaataaaaa aatctttaaa attcatgcag atacaccaga attagtagtt cgtaagaaag
    24181 atggcagtct ttctaaggga tttgactatt acatggagag agttatccct catgatggtg
    24241 atatttatta tgattttaaa gacttgattt cagctatgac atctaatcct acaggtacgt
    24301 ttattttagg gagagatatt tcctcgcgta atgtaaaacc ggatggaaat ggaaaatctt
    24361 atatcaaagg tgaatttaaa ggaaaactac ttggtacaaa tgataatgtt agacactcga
    24421 tctttgactt agaatatcca ttgtttgata ctataaaatc aggagtcgtg aaagatattg
    24481 atttcaagca tgtcaatatg gtattcccag atagcaatca aggagataac gtagcaacaa
    24541 ttgcaagagt aatcaaagat aagacaaaaa ttgagaatgt taatgttgaa ggttatctcg
    24601 agggtcgaga tcatgtagct ggtttagtta ataatcttga aggtaattcc gaaattgaaa
    24661 atgtatcctt tacaggaaag attaagtcta aaggtggtaa ttctattaca gcaggtattg
    24721 caggtagaaa tatcttatct cgtgttaaac gtgcctatgt aaatgcagat atagaggtac
    24781 atcgatcatc taattctagc atgcttgttg cagtaaatgg aataaacgca gatgcaagtg
    24841 gtggttgggg aacctggggt agacttactg agtcagttgc taaaggaaca ttagaaacca
    24901 agcaaggagg gcaagctgga ggagcctctt ctacagtctg gccttatgga gcaattgata
    24961 atgttgtcag ctatgcaaaa gtgaccaaag ggaaagaatt atttggatca gatggtgatt
    25021 taaattatga ttggtttatg aaaaaaatta gtaatatttt tggtgttcaa ggcatcagct
    25081 caggcgattc gggtagtgat tctaaattta cgcgtatttc tgaggaagaa gcaaatcaaa
    25141 aagttgcttc ttataatatt acagctccca acttgatgag tgactcaagt cttttggtag
    25201 acagactcaa tgaaagttgg aaaaatactg atcaatttga aagtatacag gattatcaag
    25261 cccaaaacca attaatttac cagaacttga ctaaatttac tccttattac aataaagaat
    25321 ttattgttca tgaaggaaat gctctcactc ctgaacagga aatattgaag actaagaaaa
    25381 tcaaatcgat tgtagggttg aaaggaacag agttcgttgt agatggcagt gatattgata
    25441 ctatcatgct tcattttgag gatggtagtc agaagcgata taaggtaacc tctactggta
    25501 agttttcgat aactaattta ccggaatatc aggttgaaga tttaaatgtg gtgtatacat
    25561 cagaacatat agtacatcca ctagattcgt cactgataaa taaccttgtt gaagaattga
    25621 aaaaagtaga attgtataca gagtctactt accaagttct aggtattgat aaagataatg
    25681 ctaacaaatt gaatagaact aagagattat tcttagatga gtcattagat gctgttaaaa
    25741 cacaattgcc tacctttgta aaaacaatgt ttgaaaatga gtggttgcac atcaatggag
    25801 aatctagtgg agctgtcgct gctttacgac aaaaaatcat ggataataag acggctatat
    25861 tgcttgcttt gacttatatc aatcgttatt atgatgttaa attctcagac tataatatta
    25921 aaaaactgat gctctttaaa ccaactttcc atggtgaaaa gattgatcta ttagatcgat
    25981 taatccgact aggatcatct ggagaaaata gattaaaagg ttcagaaaat gcagaaacgt
    26041 tcaagcagtt atttgctagc gaaacgaagc agaaggattt ggtgacttat ttagactaca
    26101 atcgctcttt gttgactaat tatcaaacaa caggggaatg gtttaaagaa acgactaagg
    26161 attatattca atttgaggaa cgtccatcct tagtagagga gattaaagat gccaaatatc
    26221 gagtttacga taatttaacc gcaccgtatt atcaaggata tatcctacct ctcttaacgt
    26281 tgaagaatac tcatttagca attttatcga actatagtac tatgacattt gtcagccgtg
    26341 aaaaacgccc taattggaaa aatgaagatt ttgataagtg ggtgaaatat gtagcaactg
    26401 ctcatagaaa ccatgttgat acttggtata agattttacc tgataatatc aaaggtaaaa
    26461 tggttaaaga gaatgttact gctgtttggg aaggattgag catcccagga agtgaatggg
    26521 tagaccagaa tgctgtagat agaaaaggaa gagattacgc cccagctcga gaattcttta
    26581 atcttgtagg tggtcctatg ggaggttggt atgcttacca tggttatggt gcgcatgctg
    26641 gaggaagaaa tcgggttaat tatgaggtgt ttgatgtatt aagtgagtat ggtatatcgg
    26701 tctttactca tgaattgact cacgttaatg atacttggat ttacttagga gggtatggta
    26761 gacgtgaaaa tatgggacct gaagcttatg ctcagggatt attccagtct cctgttccag
    26821 gtcagccggg ttggggggcg cttggtctca atatggcatt tgaaagaaaa aatgatggtg
    26881 atctaattta taatgcctcc ccaactcaat ttgaaaatag gaaagagtta gatagttata
    26941 tgaaaaatta taacgatact ctcatgatgg tggactactt ggaaggagat gcagttatct
    27001 ctaaaggtaa agaagctatt actaaatggt ttaaaaaagt tgaacctaag gttgttagtc
    27061 aaacagctca gtatgatacg gttcgtcagt tgactgctga agagaaagaa aaactttctg
    27121 tttctagtgt tgatgattta gttgatcaag gattaatgtc agacagagct gtaggaaata
    27181 atacctataa tccagctgat tttgaaacaa gttatattgc gatagattat atgactggta
    27241 tttatggtgg aggaaagaat agtgtaggtt caccaggtgc cttgatgttt aaacataata
    27301 ctttccgtat gtggggctac tatggttttg aagaaggtgt tctaggatat gcttctaata
    27361 aatttaagca agcatctaga gatgaaggtc atgctggact tagtgataac tttattattt
    27421 ctaagatttc caaaggggaa tttttaacta tggaagcatt taaaaaggga tattttaaga
    27481 aagtagtaga agagttaaaa actaaaggta ttcgtcctgt tactatcaat caaaaaacat
    27541 atagcacctt tgaagaattg caagaaggat ttaagcaggc tgttgaaaga gatttgaaga
    27601 aaaatcagtt agatgagagg gaaacaagaa acttcaaatt ccaagttttc agacaacttc
    27661 ttcaacaaac agatagcttt aaaacttcta tctttagata atcgaaatct ctcatctgct
    27721 ttgcggatga gggttttctt attttatgct atacttaaag tatgcataga aaaacagtga
    27781 ttgattttag gactttgggg gagagatata cctttaccca gcctattaaa gagttgaaaa
    27841 cgagaaatgt agaagaagtg gcagatttgc tggcacaagt ggaaagctac caagagcaag
    27901 gttattatgt ggtggggtat gtcagctacg aggctgcacc tgcttttgag gaaaaattag
    27961 cagttcacaa ggatccccta ctgggagagt acctgcttta ttttactgtt catgatagcg
    28021 tagaaacttc ccctattcct ctgacttatg aggatattga tttgccctca aattggcagg
    28081 aagtaacgtc tgcagcagac tatgaaaagg ccattgccca aattcaccat catttacggc
    28141 agggagatac ctatcaggtc aactacaccg tccaacttaa gcaaaagtta aatgccaatc
    28201 cttttgccat ctacaatcgt atggtggtag agcaggaggc gggctacaat gcctatgtgg
    28261 aacatgacga gatggcagtg atttccatga gcccagagct cttttttgag caaaatgatc
    28321 gcgagttgac aacacgacca atgaagggga cgactcagcg tggggtaact gaccaagaag
    28381 atcttgaaca ggccagttgg ttggaacagg atcccaaaaa tcgctctgaa aatatgatga
    28441 ttgtggacct cttgcgcaat gatatgaacc gtatttctga agttgggagc gagcacgtgg
    28501 agcgtctgtg tcaggtagag cagtattcaa ctgtttggca gatgacttcg accatcaaga
    28561 gtcagttgcg agaggatgtg gaccttgttg aaatcttcca ctcactcttt ccttgtggtt
    28621 ccataacggg tgcaccgaaa attgcgacaa tggagattat caaggacttg gagcctcaac
    28681 cgcgtggagt ctactgtgga acgattggtc tcttgcttcc aaatggacga cggattttta
    28741 atgtggccat tcgtaccatt caacttcaca aaggtcaagc catttatgga gttggcggag
    28801 ggattacttg ggatagcact tgggaatctg aataccgtga agttcatcaa aaggcagcag
    28861 ttctctatcg taaacaagct cgcttccaat taattacaac tggaaaaatc agccaaaaac
    28921 aactgatgtt tgaagaacaa catctggaaa ggctgagaaa agctagtcgg tattttgctt
    28981 atccttttga cccagaaatt ttgagacaaa agattgaagc agagtgtcag acttgtgatg
    29041 ctaatcaaga ttaccgcttg cgaatttctc tcagcaagtc tggagagata gaattcagtc
    29101 gtcaaatctt aacacctctt agtccaagtt tttgtcaggc caaactttgt ctgcaagaag
    29161 cgaatttgaa tcaatccttt acctatttta aaacgactta cagaccgcat ttgagcatag
    29221 aggaacaaga gaagatttac cataatggaa aaggagaact tcttgaaacc tctatcggaa
    29281 atttggttct gaaaatcgat gggaaactct acacaccgcc tatccgactt ggaatcttgc
    29341 caggaattta tcaccagtat ttactagaaa caggacaggt agaagagaaa gtcttgacct
    29401 tggcagattt tgcccaagca gaagctattt acggctgtaa tgcagtgaga ggcttgtatg
    29461 agttgcaagt gataggattg tgagttttag ctattaatag tttttaatat atattaaaat
    29521 atattggtgt ttacttttga tgagatatca tatttgtgtt ataatgtaac cgtaaacaat
    29581 agaaatttaa aggagatttt tgatgaaatc aaaaatagtt ttgggagctt cgctagcgat
    29641 cgcaacactt agcttagtat ctcttgtcga gattgaagga ctctcaccgt ttttaataga
    29701 gaatgttagc gcaaatactc attctgcaaa taaagttatt aatcataaag taagtattta
    29761 cttagaaaat gctgatgaag gtaaaggtct aactgtaaac ttttcaacag acagtgttag
    29821 tccaaattta tttgatgagt tcgaaaaaaa gagtggaatt acaataacta ctatgttagt
    29881 gaatgctaag acaggtgaag ttgtagaaaa aagattaaca ccaagcgttt ttttacgttc
    29941 caatgattta accagtggga ccatatcttc gtttattttt agtgaatatc ctgatggaga
    30001 atataaatat gttgtgtcta aaggagactt tatcgatcct aagactcagt ttaaacatca
    30061 gtatagagga gaatctccag tttttagaat acgtaatcga aaatatgtgg agttgggaac
    30121 aactgataaa aaattagatg aaagacgtga taatagcgtt tataaagatg gggttgttga
    30181 acacaaggta aatttaagtt taacaagtta tcaaggaggt aatggagtta ctgcaatttt
    30241 ttcaactgat agtgttaact ctaacttact taattctttt ggtgaaaagg ctaaaaaagt
    30301 tttgattagg tctaagttaa ttaatgttaa gactggcgag gttattgatg aaacgttttc
    30361 tccaaaagta agtttaacct ctaaaatttt gaaaagtggt tctacagcag tattttattt
    30421 tatagattta actgatggcg aatataaata tgttgcgtat gaaagccagc aatatactga
    30481 tcctcaaaca acattgactc atcagtatag aggagaatct cctattttta gtatcaaaga
    30541 tggaaaattt tctggtttag tatctgcctc aaaaccagac gaaaacccga agccaacgcc
    30601 aaaaccggat gagaaaccaa aaccttcagc accacaacag gagaaaacaa aacctacagt
    30661 tcagtctggt tgggtaggtt catcttatta tcaaaatggt aaaaaagtca ctagtaagtg
    30721 gatttttgat aaaaaatata attcatactt ctatctagat gcatcaggaa attatgttca
    30781 aaacgcttgg gtaggtaact attatctcaa atcaggcgga tacatggcta aaagtgagtg
    30841 gatttatgat aagaactatg gttcatacta ctatctaaca tcagaaggca gctacgctcg
    30901 taacacttgg tcaggcaatt attatttgaa gtcaaatggt aagatggcta aaagtgagtg
    30961 ggtatacgat agtaactaca aatcatatta ctacttgaca tcagaaggaa gttatgctcg
    31021 taacacttgg gtaggcaatt actatcttaa atcaaacggt aaaatggctg ttaatgaacg
    31081 tacaccagat ggttatcgtg tagatggttc aggtaaatgg gttaaataag aaactaaaaa
    31141 ataggataat aggtaatatc agatgaataa aaaaaattat tttcatgtgc tttagcattt
    31201 tcaacgattg gaacattgtc aacgattgat aaatcaggag ttcttccttt ttcagcgcaa
    31261 acggtgcagg ctgacataca aacgcttacc ccaatggcaa aatattatat tattcttccg
    31321 aaggatgcag aaatatataa aacttggaga ggtactgtca atattcccat tattgatgct
    31381 acaaaaacta ctccagaatt atcttatttc aaagaagatc atcgcaacta tatagctaat
    31441 gagaataagt ctggtgccaa ttacatagag tggaagggaa cagtagaaga gtttaaagag
    31501 gccattaaga agttgactga taaaaaatca actactgcaa caccaaaaaa ggatgaaaag
    31561 ccaacaccaa aaccggatga gaagccaaaa ccaacaccta cagttcagtc tggttgggta
    31621 ggttcatcct attatcaaga tggtaaaaaa gtcattagta agtggatttt tgataaaaaa
    31681 tataattcat acttctatct agatgcatca ggaaattatg ttcaaaacgc ttgggtaggt
    31741 aactattatc ttaaatcagg cggatacatg gctaaaggtg aatgggttta tgatgccacc
    31801 tatcaagctt ggtattattt gacatcagat ggttcttatg cttacagtac atggcaagga
    31861 aattactatc taaaatcgga tggtaaaatg gctgtcaatg aatgggttga tggtggacgt
    31921 tattatgttg gcgctgacgg agtttggaag gaaggtcaag caagtacagc ttcttctagt
    31981 aatgatagca atagtgaata ttctgctgct ttaggaaagg caaaaagtta taattcgtta
    32041 ttccacatgt caaaaaaaaa cgtatgtata gacaattaac ttctgatttt gataaatttt
    32101 caaatgatgc agctcaatat gccattgatc attagatgat taacagatac tggtaggcga
    32161 aaaaattcct tataatccat ttacgcaaac ctttgcataa gtgaatggat tttgatatac
    32221 tagtactgta agcattttca attactacac aatgaaaaag gagaatatga tgagtcaaaa
    32281 gattattggg attgaccttg gtggaacttc tatcaaattt gcaatcttaa caacagcagg
    32341 agaaatccaa ggaaaatggt caatcaagac caacattttg gatgagggaa gtcatatcgt
    32401 tgatgatatg attgagtcta ttcaacatcg tttggacttg cttggattgg cagcagcgga
    32461 cttccaaggc attggaatgg gatcaccagg tgtggttgac cgtgacaaag ggactgttat
    32521 cggtgcctac aacttgaact ggaaaaccct tcaaccaatc aaacaaaaga ttgaaaaagc
    32581 tttgggcatt ccatttttca tcgataatga tgccaacgta gcagctcttg gtgagcgctg
    32641 gatgggtgct ggagataacc aaccagacgt tgtctttatg acactcggta ctggtgttgg
    32701 tggcggtatc gtcgcagaag gcaaattgct tcacggtgtt gctggtgcag caggtgagct
    32761 tggtcacatc actgttgact ttgaccagcc aatctcatgt acttgcggta agaaaggctg
    32821 ccttgagaca gttgcttcag caacagggat tgtcaacttg actcgtcgct atgccgatga
    32881 atacgaaggc gatgcagcct tgaaacgctt gattgataat ggagaagaag taactgctaa
    32941 gactgtcttt gatctcgcaa aagaaggaga cgaccttgct ttgattgttt accgtaactt
    33001 ctcacgttac ttgggaatcg cttgtgctaa cattggttca atcctaaacc catcaacaat
    33061 cgtcatcggt ggtggtgtgt cagctgcggg agaattcctt ctacaaggtg ttcaaaaagt
    33121 ttacgatgaa aatagtttcc cacaagtacg cacatctact aaattagctc ttgcaactct
    33181 aggaaatgac gctggagtta tcggagcagc atcacttgta ttgcaataag ataatgagag
    33241 aggaaaatgc taacctagag ttagtaatgt tcctctttta ttttttatgc tatactagtt
    33301 aggacaatga atgaggtgag agtaaatgac aaaagcagat acgattttta aagagaatat
    33361 tgaatgcatt ctaaaggaag gtgttttctc agagcaagct cgtcctaagt acaaggatgg
    33421 gactgttgct aattccaagt atgtaactgg tgccttttcc gagtacgact tatctaaagg
    33481 agaatttcct atcacaacct tgcgtcccat tgcaatcaaa tccgccatca aagaagttct
    33541 ctggatttac caagaccagt caaatagctt agaagtgctt aatgacaagt acaatgttca
    33601 ctactggaat gactgggaag ttggagacac gggaaccatt ggtgagcgct atggtgccgt
    33661 tgttaagaaa cacgacatta tcaataagct tctcaaacag ttggaaacca atccttggaa
    33721 tcgccgcaat attatttcgc tctgggatta ccaagctttc gaagaaacag atgggctgct
    33781 cccgtgcgcc tttcagacca tgtttgatgt ccggcgtgtt gatggggaaa tctatctgga
    33841 tgcgaccttg acccagcgct ccaatgatat gctggtggcc caccacatca acgctatgca
    33901 gtatgtggct ttacagatga tgattgccaa acattttggt tggaaggttg ggaagttctt
    33961 ctatttcatc aacaacctcc atatctatga taatcaattt gaacaagctc aggaattgct
    34021 ccgtcgggag ccgtcaaact gccaaccacg cttggtttta aatgttcctg atgggactaa
    34081 tttctttgat atcaaagcag aagattttga gttggtggat tatgaccctg ttaagccaca
    34141 gttgaagttt gacctagcta tttaaaagaa tagaaaaaag aagttgagaa taatcccaac
    34201 ttcttttgtt tcttaacgtg atacgcggcg acgagctgct tttttacgat tttcttcgat
    34261 gaaagctgct ttttgctctt ctggttcgat tactttcttt ttaaatgcgt atactgcacc
    34321 tgcaacggca gcgacagttc ctgcgacacc tgttacaaga cctttagcga atcctttagc
    34381 catgagtctt cctcctttat attctcaatc agccagcctc ctcaagaggt cacatttttc
    34441 tgactgacct ttttgtgtta taataatagt aacgaaaaaa tgggaatttt tcaaggaaaa
    34501 aagatgaaaa caaaaataat tgtgattgtt ggaccgactg ctgttggaaa gacggctcta
    34561 gctattgaag ttgcaaagcg ctttaatggc gaagtggtta gtggagatag ccagcaagtc
    34621 tatcgaggac ttgatattgg gacggccaag gctagtccag aagagcaggc agctgttcct
    34681 catcatttaa tcgatgttag agagataacc gagtcttact cggcttttga ttttgtttca
    34741 gaagctaaga tgactattga ggatattcag agccgtggca agctagccat tatcgccggt
    34801 gggactggac tttatatcca gagcttgcta gaaggttacc acctaggtgg ggagactcct
    34861 catgaggaga ttttagctta tcgagctagt ttggagccat attcagatga ggaattagcc
    34921 catttggtgg agcaagcagg ccttgagatt ccccagttta atcgtcgtcg tgctatgcgt
    34981 gccttagaaa ttgcccattt tggtcaggat ttggaaaatc aagagatctt gtatgaaccg
    35041 ctgattatct gcttggatga tgaacgtagt caactttatg agcgtatcaa ccaccgagtg
    35101 gatttgatgt ttgaggctgg gcttttggat gaggccaagt ggctgtttga ccattcccct
    35161 aatgtacagg ctgctaaagg aattggctat aaggaactct ttccttattt ccgtggagag
    35221 cagacctttg aggaagctcg tgagagtctt aaacaggcga cccgtcgttt tgccaagcgt
    35281 cagctgacct ggttccgtaa tcgcatgcag gtcacctttt atcagattgg agaatctggt
    35341 gtacaagacc gcattttaag ccagatagag gagtttttag atgattgaaa cggagaaaaa
    35401 agaggagcga gtcctgctga ttggtgtgga attgcagggt atggacagtt ttgacctctc
    35461 catggaagaa ttggctagtt tagcgaaaac ggcaggggca gtcgttgtag atagctacag
    35521 acaaaaacgt gaaaaatatg attccaagac cttcgtcggc tctggtaagt tggaagagat
    35581 tgcgcttatg gtggatgcag aagaaatcac tactgtcatc gtcaacaatc gtctgacccc
    35641 aaggcagaat gtcaatctag aggaagttct cggtgttaag gtcattgacc gtatgcagtt
    35701 gattttggat atctttgcca tgcgggctcg aagccatgaa gggaagctcc aagtccacct
    35761 agcccaactc aaatacctct tgcctcgctt ggttggtcag gggattatgc tcagccgtca
    35821 ggcaggggga attggttccc gtggtcctgg tgaaagccaa ctggagctga accgtcgtag
    35881 cgttcgcaat caaatcacgg atatcgagcg ccagcttaag gtggttgaga aaaatcgtgc
    35941 gactgtcaga gaaaaacgtt tggagtctag cacttttaag attggtttga ttggttatac
    36001 taatgctggg aaatcaacta tcatgaacat cttgaccagt aagacccagt atgaagcaga
    36061 tgagctcttt gcgactctgg atgcgacaac caagagtatt catctgggag gcaatctcca
    36121 agtaactttg acagataccg ttggctttat ccaagatttg ccgacagagt tggtgtccag
    36181 tttcaagtca accttggaag aaagcaagca tgtggacctt ctggttcatg ttatcgatgc
    36241 tagcaatcct taccacgagg agcatgaaaa aacggttctc tccatcatga aagacctgga
    36301 catggaagat attcctcact tgacgcttta taataaagcg gatttggtgg aggatttcac
    36361 gcctacccaa acgccatata ccctcatttc tgccaagtct gaggacagtc gtgaaaactt
    36421 gcaagcatta ttgctagata agattaagga aatttttgaa gcatttaccc tgcgagtgcc
    36481 tttttcaaag tcctacaaga ttcatgattt agagagtgtt gcaattctgg aagaacgtga
    36541 ttatcaggaa gacggcgaag tgattacagg ctacatttcc gagaaaaata aatggaggtt
    36601 agaagaattt tatgactgat attaaaactt tggctctaaa gtatggaggt tatacaagtc
    36661 tggacaaggt ctatctggat cagcttctag ctggtaaaac agatcaggag cagttaacac
    36721 ttattacacc tccgccaagt gttgttaatg cctactttgc agaactctat cagaaaaaga
    36781 gtcctcaggc tgcgacggat tattttgcag aactcagtca ggaactgaat ctttacaata
    36841 ctgagccaag tttcacccta gaaaataagc cttttattcg gcttaatctg tctggtaaat
    36901 cctttggttt ttgttatgag agtgacggcc ttggtcggat tttctttgaa aatgaggaga
    36961 aaatctcgga tgacttgctc tttgaaattg cgcaaatttt cccccatcaa ttagtctttg
    37021 aagagtctgg taagatttac atgaagcctg tcgaggacga ggaagttatc agcctagaaa
    37081 gtctcacagc tttgactgat ttggaaagat tggctgatgg tcgaaaacgt ctcaaaggct
    37141 acagccaaga ggatttattg caagaagctg ctgctttttc tggcaaacgc tatttccgat
    37201 cggaaaaccg cacagccatg ttatatattg attaattaga aagtatcgaa tggatattca
    37261 atttttagga acgggggctg gtcagccctc taaagcccgc aacgtttcaa gtctcgccct
    37321 gaaacttttg gatgagatta acgaagtttg gctctttgac tgtggagaag gtacgcaaaa
    37381 tcgcattctg gaaaccacaa ttcgaccacg taaggtcagc aaaatcttta ttacccatct
    37441 gcatggagac cacatttttg gtttgccagg tttcctttct agccgtgcct ttcaggccaa
    37501 tgaagagcag acagatttgg aaatctacgg acctcaagga atcaagtcat ttgtcttaac
    37561 cagccttcgt gtgtcaggtt ctcgtctgcc ctaccgcatt catttccatg agtttgacca
    37621 agattctctg ggtaaaattc ttgaaaccga taaattcact gtgtatgcag aggagctgga
    37681 ccacactatt ttctgtgttg gctatcgtgt catgcaaaag gatctagaag ggacgctgga
    37741 tgctgaaaaa ctcaaggctg ctggtgttcc gttcggcccg ctttttggta aaatcaaaaa
    37801 cggccaggat cttgttttgg aagacggaac tgaaatcaag gcagcagact atatctcagc
    37861 gccacgtcca ggtaagatta tcactatttt aggagacact cgaaaaacgg gtgccagtgt
    37921 gcgtctggct gtcaatgcag atgtcctagt tcatgagtcc acttatggca agggtgatga
    37981 aaaaattgct cgtaaccatg gtcactcaac taatatgcaa gctgcacaag tagcggtaga
    38041 agcaggtgcc aaacgcctcc tactcaacca tatcagtgcc cgtttcctct caaaagatat
    38101 tagcaaactc aagaaagacg ctgccacaat ttttgaaaat gtccatgtgg tcaaagactt
    38161 ggaagaagtg gaaatctagc agtcacagaa aggataagta tgcctactat tctcattacc
    38221 ggagctagcg gtggtctagc tcaagaaatg gtcaaactcc tgcccaatga ccaactcatc
    38281 ttgcttggta gaaataagga aaaattagcc caactctacg gaaattattc ccatgcagaa
    38341 ttgattgaaa ttgatattac cgacgattca gccctagaag ctctggtaac tgatctttat
    38401 ctccgctatg gcaagattga tgtcttgatt aacaacgctg gttacgggat ttttgaggga
    38461 tttgaccaga ttgctgataa agatattcac cagatgtttg aggtcaatac ctttgccctg
    38521 atgaatctgt ctcgtcacct tgcggctcgt atgaaggaaa gcagcaaagg gcatatcatc
    38581 aacatcgtca gcatggcagg tctaatagct actggcaagt ctagtcttta ctcagcgacc
    38641 aagtttgcgg ctattggttt ttcaaatgct ctgcgactcg aacttatgcc ctatggagtc
    38701 tatgtgacaa cagtcaatcc aggtccaatc cgaacaggat ttttcgacca agccgaccca
    38761 gatggaactt atcttaaatc ggttgaccgc ttcctgctag aaccagatgc agtggctaaa
    38821 aagattgtca agattatagg aaaaaacaaa cgagaactca atctcccgat tttgttgaac
    38881 ctagctcata agttttatac cctctttcct aagctagctg ataagttggc aggggaaact
    38941 tttaattata agtaaaaaga accaatgtgc aggttgttgc tagcctacat attggttctt
    39001 atcatttctc aactatcaaa ctgaacttct tctaggagat agtctataaa gcgttcgccc
    39061 atcttagata ggctggtttt ctcatgctgg atatagacca gctcgatcgg gtcatcaata
    39121 tccagtggga tagaaacgat attgtctccg tttaggttgc tgttcaaaat ccctgtcgca
    39181 atggtataac catccaaacc aatcaagaga ttaaagaggg tggcacggtc actgaccaca
    39241 atggatttct tgtggtgttc ttgagaaaga atctcttctg aaaagtagaa ggagttgtgc
    39301 gtcccttggt catagctgag gtaagggaaa ttctccaaat cagacagttt caccttgtct
    39361 ttctttgcca gagggttggt cttgctgaca aagatatgtg gttgcgctgt gaagagatgg
    39421 tgggctagca ggtgattgtc atccagcatc ttggttaaaa catcacggtt gtaactgttt
    39481 aagaagagga ccccgacctc actgcggaag ttcttgacgt cgtcgataat ctcccaagtc
    39541 cgagtttcac gaaggaagag ttcgtatttc tccatatcgc ttttcttgag caaagagaca
    39601 aaggcattga ccacaaaggc atagtgttga gacgaaacgc taaatagttc gcggtgggcg
    39661 acaggatttt tatagcgttc ctccagaagc tgggtctgct cgacaacctg acgggcataa
    39721 gagagaaact ccatcccatc acgggtcaag gtgattccct tgggattgcg gataaagatc
    39781 tcaatgccca tttcattttc caaatctcgc actgcattgg agagacttgg ctgagtgata
    39841 aagagttgct tggctgcctc attcatggag ccagtttcga cgattttgat aatataatgt
    39901 aattgttgaa ttctcatgtt tttattgtac catacttgcc ttagaatcgc taaagaagat
    39961 aggaaaataa ggagcattgt gtgagtctct tcttgaaaaa aacacaaaat tttgctagaa
    40021 tgagtcttat gaaaacattc tatgatgtgc agcaattcct caaacgattt ggtattattg
    40081 tttacatggg aaaacgctta tatgatattg aactgatgaa gttggaacta tctcggattt
    40141 acgatgcagg gttgatggat aaattagact atctagaggc agaagcagtt cttcgcagag
    40201 agcacaaggt agaattggat tatattgaga aaaatggaga aaagaactaa tggttacttg
    40261 gattttgtgg gcacttatac tagcaatgtt ggcgtggatg ggctttaact atcttcgtat
    40321 tcgccgtgcg gctaaaattg tggacaatga ggagtttgaa gccttgattc gtacgggtca
    40381 attgattgat ttgcgcgacc cagcagaatt ccacagaaaa catatccttg gtgcacgcaa
    40441 tattccttca agtcagttga aaactagtct tgcagccctt cgtaaagata aacctgtcct
    40501 tctctacgag aaccaacgtg cgcaacgagt tacaaatgca gctctttact tgaaaaaaca
    40561 aggtttttct gagatttata tcctttctta tggcttggat tcttggaaag ggaaagtgaa
    40621 gactagctaa ccttatttcc aaattagtcg aatgatagga tgaagctatt gttttatagt
    40681 ataattaatt tgtataaaat ttaaggagtt cttagaacat aaaagaaaag aagaaaaatc
    40741 cactttttgt gggtatcttg tcgattattc ttggtttgtt atttccaatt gtaggcctga
    40801 ttttgggtat cataggattg gtcttggcta tttcatacca aaaagagtca taattagact
    40861 ataaaataga aaagattctt aatatcttag gaattgtgat ttctgtagtt aactggattg
    40921 tagcaatcgc cttaattttt cggtaaataa aagaaaaaag ctttagtttt aaagcttttt
    40981 ttgttttatc gactcatctc taaatagttt ttaatgattt gtaactcttt ttggttcaaa
    41041 gggcggtagt tactttctgc taaatctggg ttcaatgtga agtccccaaa ttggattctc
    41101 ttgaggatag tcaccttaac accaaccgag aggaacattt tcttgatttg atgaaatttt
    41161 ccttctgaaa tggtgataga ggcttgactg atggagggac ttgcagatag aatctctagt
    41221 tttgcgggtt tacagacagt accatctaaa aagacaattc ccttttgaaa ggcttggata
    41281 tggtcaggtg ttagaagtcc attaacctca acttgataag tcttatcgac atgatattgg
    41341 ggatggagga gttggaagcc caagggaccg ttatcggtca agaggaggag tcccgttgta
    41401 tctcggtcca gtcggccaac ggcatagagc ttgtcagact ggatgtcaga tggaagcagg
    41461 tccatgacgg tcggaagttc cttgtctttg ttggctgtaa cgacaccagc aggtttatga
    41521 agcataagat aggtgtgttc atagccttga atgattcgat cctgaaaaag gagttcttgt
    41581 agtcctgtat cgatattttg agctagggag cgggctgggc aaccatcgac tagaattttc
    41641 cctctgagga gtgcttgctt catggccttt cggctgattt tttcttgggc taataaatta
    41701 tctaaacgca tactgtgcct atcttatcat aagtcgcttg ctttgaaaag gcttgacgtg
    41761 aaaagaaaag cggagtgaaa acatttacac ttgcttgagt tatgttattt atttgaaatt
    41821 atggtataat cgttcagtta gaaaataaat tttgaatatt atagaggaaa tcatgacaaa
    41881 attaagagaa gatatccgta acattgcgat tatcgcccac gttgaccacg gtaaaacaac
    41941 cctggttgac gaattattga aacaatcaga aacgcttgat gcacgtactg aattggcaga
    42001 gcgtgctatg gactcaaacg atatcgaaaa agagcgtgga atcaccatcc ttgctaaaaa
    42061 tactgccgtt gcttacaacg gaactcgtat caacattatg gacacaccag gacacgcgga
    42121 cttcggtgga gaagttgagc gtatcatgaa aatggttgac ggtgttgtct tggtcgtaga
    42181 tgcctatgaa ggaaccatgc cacaaactcg tttcgtattg aaaaaagcct tggaacaaga
    42241 ccttgtccca atcgtggttg ttaacaaaat cgataagcca tcagctcgtc cagcagaagt
    42301 agtggatgaa gtcttggaac ttttcatcga gcttggtgca gatgacgacc agcttgattt
    42361 cccagtggtt tatgcttcag cgatcaacgg aacttcttca ttgtcagatg atccagctga
    42421 ccaagaagcg actatggcac caatctttga cacgattatc gaccatatcc cagctccagt
    42481 agataactca gatgagcctt tgcagttcca agtgtcactt ttggactaca atgacttcgt
    42541 tggacgtatc ggtatcggtc gtgtcttccg tggtacagtt aaggttgggg accaagttac
    42601 cctttctaaa cttgacggta caactaaaaa cttccgtgtt acaaaactct tcggtttctt
    42661 tggtttggaa cgtcgtgaaa tccaagaagc caaagcgggt gacttgattg ccgtttcagg
    42721 tatggaagac atctttgtcg gtgaaaccat cactccgaca gatgcagtag aagctcttcc
    42781 aatcctacac atcgatgagc caactcttca aatgactttc ttggtcaaca actcaccatt
    42841 tgctggtaaa gaaggtaaat gggtaacttc tcgtaaggtg gaagaacgct tgcaggcgga
    42901 attgcaaaca gacgtttccc ttcgtgttga cccaactgat tcaccagata aatggactgt
    42961 ttcaggacgt ggagaattgc acttgtcaat ccttatcgaa acaatgcgtc gtgagggcta
    43021 tgaacttcaa gtatctcgtc cagaagttat cgtaaaagaa atcgacggtg ttaaatgtga
    43081 accatttgaa cgtgtacaaa tcgacactcc agaagaatac caagggtctg ttatccaaag
    43141 cctttctgaa cgtaaaggtg aaatgttgga tatgatttca actggtaatg gtcaaactcg
    43201 tttggtcttc cttgttccag cgcgtggttt gattggatac tcaactgagt tcttgtcaat
    43261 gactcgtggt tacggtatca tgaaccatac cttcgaccaa tacttgccat tgattccagg
    43321 ggaaattggt ggacgtcacc gtggtgccct tgtttctatc gatgctggta aggctacaac
    43381 ttactcaatc atgtctatcg aagaacgtgg tacgatcttt gtcaacccag gtactgaggt
    43441 ttatgaagga atgatcatcg gtgaaaactc tcgtgaaaat gacttgacag ttaacatcac
    43501 gaaggcaaaa caaatgacca acgttcgttc agctactaag gaccaaacag ctgttatcaa
    43561 gacgcctcgt atcttgacac ttgaagagtc tcttgagttc ttgaacgacg atgagtacat
    43621 ggaagtaacg cctgagtcta tccgtttgcg taaacaaatc cttaacaaag cagagcgtga
    43681 gaaagctaac aagaagaaaa aatcagctga ataagagcta gaaagagaga aagatggtct
    43741 atttagtcct aggaatttta ctgctcctac tctatgtatt tgcgacacca gaaagcatta
    43801 aagggacagt caatatcgtc gctatggtat gtattttagt ggcactcttg attttattgg
    43861 ttctatcttt tctgaaaatt tttcaattac caacagaaat attcctagca atagccatgt
    43921 tgctcctagc ttactttagt gttagagaca ttacactcat gccagtcaaa aaaagtaaaa
    43981 gaagataaaa aagagagttt tggctctctt ttttgctagg aattttacaa gattgcaatt
    44041 tatttttgag ttatcaccat ctcttttgtt tggataatat gaatgggaaa aacttgaata
    44101 aaattcgtta atggaatcta ttttatattt ggtt
//
LOCUS       DAVLVF010000008        40701 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVLVF010000008 DAVLVF010000000
VERSION     DAVLVF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.008"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            58..339
                     /locus_tag="VYN66_000389"
     CDS             58..339
                     /locus_tag="VYN66_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV1494468.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            471..1415
                     /gene="manA"
                     /locus_tag="VYN66_000390"
     CDS             471..1415
                     /gene="manA"
                     /locus_tag="VYN66_000390"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV1494469.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(1437..2912)
                     /locus_tag="VYN66_000391"
     CDS             complement(1437..2912)
                     /locus_tag="VYN66_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEV1494470.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMNFLVPNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVPSFQSSSWSSSPNLCNQKGLE"
     gene            3078..3242
                     /locus_tag="VYN66_000392"
     CDS             3078..3242
                     /locus_tag="VYN66_000392"
                     /inference="COORDINATES: protein motif:HMM:NF024803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1494471.1"
                     /translation="MVPLKSENGYRTYSQEDLERLQVILYYKYLGFSLEKIAELLKEE
                     RTDLLLHLTR"
     gene            3243..3749
                     /locus_tag="VYN66_000393"
     CDS             3243..3749
                     /locus_tag="VYN66_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HEV1494472.1"
                     /translation="MDYLTRERQHLDTLISTLQKTIQEQKGERKMTIEEKFTGFSYQD
                     NQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAFNQVFQTLAQNLQVGLPATATENQ
                     EQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEFKENIDKFGSETAQYTSDAIAAYV
                     QTNAEKIG"
     gene            complement(3769..4224)
                     /locus_tag="VYN66_000394"
     CDS             complement(3769..4224)
                     /locus_tag="VYN66_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1494473.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4244..5419)
                     /locus_tag="VYN66_000395"
     CDS             complement(4244..5419)
                     /locus_tag="VYN66_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1494474.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFTGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5478..6323)
                     /locus_tag="VYN66_000396"
     CDS             complement(5478..6323)
                     /locus_tag="VYN66_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1494475.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6448..7005
                     /locus_tag="VYN66_000397"
     CDS             6448..7005
                     /locus_tag="VYN66_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1494476.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7024..7491
                     /locus_tag="VYN66_000398"
     CDS             7024..7491
                     /locus_tag="VYN66_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV1494477.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7580..8209
                     /gene="upp"
                     /locus_tag="VYN66_000399"
     CDS             7580..8209
                     /gene="upp"
                     /locus_tag="VYN66_000399"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV1494478.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8383..8973
                     /gene="clpP"
                     /locus_tag="VYN66_000400"
     CDS             8383..8973
                     /gene="clpP"
                     /locus_tag="VYN66_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV1494479.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9052..9300
                     /locus_tag="VYN66_000401"
     CDS             9052..9300
                     /locus_tag="VYN66_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV1494480.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9401..10561
                     /locus_tag="VYN66_000402"
     CDS             9401..10561
                     /locus_tag="VYN66_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1494481.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            10829..11698
                     /locus_tag="VYN66_000403"
     CDS             10829..11698
                     /locus_tag="VYN66_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1494482.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11702..12658
                     /locus_tag="VYN66_000404"
     CDS             11702..12658
                     /locus_tag="VYN66_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1494483.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12658..13422
                     /locus_tag="VYN66_000405"
     CDS             12658..13422
                     /locus_tag="VYN66_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494484.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13422..14132
                     /locus_tag="VYN66_000406"
     CDS             13422..14132
                     /locus_tag="VYN66_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494485.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14546..15202
                     /locus_tag="VYN66_000407"
                     /pseudo
     CDS             14546..15202
                     /locus_tag="VYN66_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
     gene            15310..16405
                     /gene="prfB"
                     /locus_tag="VYN66_000408"
     CDS             join(15310..15381,15383..16405)
                     /gene="prfB"
                     /locus_tag="VYN66_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV1494486.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16423..17115
                     /gene="ftsE"
                     /locus_tag="VYN66_000409"
     CDS             16423..17115
                     /gene="ftsE"
                     /locus_tag="VYN66_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV1494487.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDIPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17108..18034
                     /locus_tag="VYN66_000410"
     CDS             17108..18034
                     /locus_tag="VYN66_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1494488.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKV"
     gene            18320..20500
                     /locus_tag="VYN66_000411"
     CDS             18320..20500
                     /locus_tag="VYN66_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1494489.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            20552..21367
                     /locus_tag="VYN66_000412"
     CDS             20552..21367
                     /locus_tag="VYN66_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1494490.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            21507..22850
                     /locus_tag="VYN66_000413"
     CDS             21507..22850
                     /locus_tag="VYN66_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1494491.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            23066..24256
                     /locus_tag="VYN66_000414"
     CDS             23066..24256
                     /locus_tag="VYN66_000414"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV1494492.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSEEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIKK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            24809..25744
                     /locus_tag="VYN66_000415"
     CDS             24809..25744
                     /locus_tag="VYN66_000415"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV1494493.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            25778..26815
                     /locus_tag="VYN66_000416"
     CDS             25778..26815
                     /locus_tag="VYN66_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1494494.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            26988..27593
                     /locus_tag="VYN66_000417"
     CDS             26988..27593
                     /locus_tag="VYN66_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV1494495.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELTAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            27750..28280
                     /locus_tag="VYN66_000418"
     CDS             27750..28280
                     /locus_tag="VYN66_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV1494496.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            28301..29203
                     /locus_tag="VYN66_000419"
     CDS             28301..29203
                     /locus_tag="VYN66_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HEV1494497.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVSCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDID
                     AACGQLRFNTMKRDRQKAVAAVNP"
     gene            29203..29685
                     /locus_tag="VYN66_000420"
     CDS             29203..29685
                     /locus_tag="VYN66_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1494498.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQPL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            29687..31228
                     /locus_tag="VYN66_000421"
     CDS             29687..31228
                     /locus_tag="VYN66_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1494499.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGIMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(31284..32087)
                     /locus_tag="VYN66_000422"
     CDS             complement(31284..32087)
                     /locus_tag="VYN66_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1494500.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(32171..32384)
                     /locus_tag="VYN66_000423"
                     /pseudo
     CDS             complement(32171..32384)
                     /locus_tag="VYN66_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32863..33072)
                     /locus_tag="VYN66_000424"
     CDS             complement(32863..33072)
                     /locus_tag="VYN66_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494501.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            33242..33514
                     /gene="rpsP"
                     /locus_tag="VYN66_000425"
     CDS             33242..33514
                     /gene="rpsP"
                     /locus_tag="VYN66_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV1494502.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            33534..33773
                     /locus_tag="VYN66_000426"
     CDS             33534..33773
                     /locus_tag="VYN66_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV1494503.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            34350..35138
                     /locus_tag="VYN66_000427"
     CDS             34350..35138
                     /locus_tag="VYN66_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1494504.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPKMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            35169..35687
                     /gene="rimM"
                     /locus_tag="VYN66_000428"
     CDS             35169..35687
                     /gene="rimM"
                     /locus_tag="VYN66_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV1494505.1"
                     /translation="MNYFNIGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            35677..36396
                     /gene="trmD"
                     /locus_tag="VYN66_000429"
     CDS             35677..36396
                     /gene="trmD"
                     /locus_tag="VYN66_000429"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV1494506.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDDPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            36408..36746
                     /locus_tag="VYN66_000430"
     CDS             36408..36746
                     /locus_tag="VYN66_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494507.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(36777..37097)
                     /locus_tag="VYN66_000431"
     CDS             complement(36777..37097)
                     /locus_tag="VYN66_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494508.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAKKNR
                     FKKE"
     gene            37195..37410
                     /locus_tag="VYN66_000432"
     CDS             37195..37410
                     /locus_tag="VYN66_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV1494509.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(37479..37565)
                     /locus_tag="VYN66_000433"
                     /pseudo
     CDS             complement(37479..37565)
                     /locus_tag="VYN66_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(37616..38152)
                     /locus_tag="VYN66_000434"
     CDS             complement(37616..38152)
                     /locus_tag="VYN66_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV1494510.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            38578..38916
                     /locus_tag="VYN66_000435"
     CDS             38578..38916
                     /locus_tag="VYN66_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494511.1"
                     /translation="MKKRINKKASVFLVASVFSFSFATTASVVNAVTAHPAPSPIIDV
                     WEYGVNDNYGYSNYFVSSPSNIGSKSSVTNFWGTVKAQDKKDYGWAYSSATKSWNDVR
                     LNAYWDYYRF"
     gene            38982..>40701
                     /locus_tag="VYN66_000436"
     CDS             38982..>40701
                     /locus_tag="VYN66_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1494512.1"
                     /translation="MKLKLCIIGFFFCLIATIGLVTISDTEIPIPLPIDGAFSIQGKS
                     NLSNNEIYEMVRDLSKTEKVTIYKPIVQSSGQLKYVNFDDVNNEQLKSAPIVGMYYTL
                     GKMDVDSLKPLTMTGLQTVYMAYPWYIGGILQFTGTLRILLMGSIYLTLLVVLFVVRT
                     RQIKEGVIRRSLGLPIYDLRREYGISLIFELIMMALLMISYSSFLGNGFFTYSSKLFF
                     SLLLTNFILFQIIDLITFVLFWLTIQIEKPIEIIKNKAKNKLIFVVWLAIISIIILVS
                     GVFLQETKSSQSSINIQIQNLVPWDTVKDWRRIEFLGIESNSTKNREVNDSDGQYLQI
                     VAALKNLDFLYIERSSAYVPDFMKTSHVIENFSKQLENDGITNPEINKELIYINQTGA
                     NLQNKVNGTNYHLLDNKIATIYIPEKWKENQKSIENTVVAEQFIGTNYTREQLAVQII
                     PDGEKIFYFNEDADNNLKMKDILPLANVADSKDNIVVVLDTDKMMENNKFSLASNILY
                     KSLFSPEAVKKINEMTVLLNFSMNPVDVYQIVKLKIQSLEHQILLSQILQKIIYSIVF
                     ILIYQYV"
BASE COUNT        12661 a         7378 c         8721 g        11941 t
ORIGIN      
        1 ttatataatg ctttttgatt ttagtatagt attatcgtag aaaatggact cggtgctatg
       61 gatacaccgg atgagaatgg ttatgtagca gatgactatc ggattactta cttagaggcc
      121 cacatcaagg ccatgcgaga tgccatttac aaagacgggg ttgacttgct tggttatacg
      181 acatggggct gtatcgattc ggtttcagct ggcacagggg aaatgaataa gcgctatggt
      241 tttatctatg tagatcgaga taatgtagga aatgggactc tcaaacgtag caagaagaag
      301 tccttctatt ggtacatgtc atttatagca atggtgtaag catcgagtag agcacatttg
      361 tttactattt ttataaaatc ttctccctgc tttataagat gtaaaaaaga gttcaattcg
      421 aactcttttt ttgctataat gaggggagaa aaatcagaca ggagattaag atgtcagaac
      481 cattattttt acaatcagtt atgcaagaaa aaatctgggg tggtaccaag ctacgtgatg
      541 agtttggcta cgacatccca agtgaaaaag tcggagagta ctgggctatc tcagctcatc
      601 taaatggtgt ttctaaagtt gccaatggtc gttacgaggg aacagatctt gctactttgt
      661 atgcggaaca ccgtgaatta tttggcaatc gtcctgagcc tgtatttccg cttttgacta
      721 agattttgga tgctaacgac tggctcagcg ttcaagttca cccagacgat gcttatggac
      781 tagagcatga aggcgaactt ggaaaaacag aatgctggta catcatcgct gcggatgaag
      841 gttcagagat tatctacggc cacaatgcca agtcaaaaga agaactccgc cagcaaatcg
      901 aggataagaa ctgggatgcg ctgttgacaa aagtgccagt taaagcagga gatttcttct
      961 atgtaccaag cggtaccatg cacgctatcg gggcgggtat cttgattctt gaaacccagc
     1021 agtctagcga taccacttac cgtgtctatg actttgaccg taaggatgac aagggcaatt
     1081 tacgtgaact tcaccttgaa aaatctatcg atgtcttgaa cattggtgaa cccgcaaata
     1141 gccgtccagt gacaatcaaa gcagatgatc tacgttccac tctccttgta tccaatgatt
     1201 tcttcgcagt ttacaaatgg gaaattactg gaaaagttaa ctttgaaaag acagctgact
     1261 acagcttgtt gagtgtcttg gctggtcaag gtcaactaac tgttgacggg aaaaactatc
     1321 caatccaaaa agggagacac tttatcctac caagtgatgt tgaagcttgg actctggaag
     1381 ggcaagattt agaattaatt gttagtcatc cataaaaaag aaagggcctg agttcattac
     1441 tcaagtcctt tttgattaca taaattgggc gatgaagacc acgatgatga ttggaatgac
     1501 ggaacgaaga aacgaagaag gaagagccag acttggaaca gtccttgttt ccatgctctt
     1561 tcatcgagat ggagttcctc cattgcaaga gcctttttaa agatatagcc tgtaaaaagt
     1621 gaaaggtaga gagctccaaa tggcatgagg agattgggaa ccaagaagtt catagcgtca
     1681 aagaaggtct taccaaaaat gtgaacatcc gccatgacac cgtaagatag ggctgaagga
     1741 atgccaaaga caaaggtcaa aattcctaaa ataacactcc atttggcacg tttgctgtta
     1801 tcctggttgg tgatattgcc cacattgatc tccagcatca cgacagaaga agtgacagtc
     1861 gcaaagagga agaggacgta gaaaatggtt ccaaaaggca tcttgtcaaa gagttgaggc
     1921 aagacgataa agagcaagct cggtccccct tcagactgga tattgaaggg ggaccgagct
     1981 tggaaaatgg ctagacctgc catgatggat atcgagatat tcatggctac gatggagatt
     2041 cctgactgga ctagattggt tttcttgtcc aagtaagaag cataggtcaa catgactgta
     2101 acccctagtg agagggcaaa gaaagattgt cccagagcat agaggagacc agcgctagtc
     2161 agttttgaaa agtctggttt gaggaagtaa agaacccctt ccatggcatt tggcaaactg
     2221 agagagcgac cgatgataac aacaaagatg ataaagagca ggggcatcat gactttcgaa
     2281 gctctttcaa tccctttttg aaccccacgt gatacaataa agatatttaa taagataaag
     2341 gctgcttgag ctcctagggc aatggctgga tttgaaatga ttgaagtaaa taactgagca
     2401 taatcacccg ttccaccaag ttggaacaat ttcccaaact caatacctag atagactaga
     2461 atccatcctc cgataacact gtaaaaagat aagaggataa agagggcaaa ggcgccaatc
     2521 caaccgataa agttgtactt gttattcttg cccagttttc caaaggtttt gatagcggaa
     2581 acgccagcac tacggccaag ggcaaactca gccagcagga gagggaaacc gattaaaata
     2641 gtggaaatga gaaagattag taaaaagcct ccaccgccat tagcagcagt catgtaggga
     2701 aacttccaaa cggaaccaag cccgatgacc gagccagcag atgctagaat aaagccaagt
     2761 ttcgaccccc attgtgattt ttctgacata gttaaactct gatctccttt ttcaaaataa
     2821 gaaaaagcta cttgagttgt caaatagcct tctgtttgat tgatagactt gactatccta
     2881 ttatgttttc taaggtctgt caagaaaaca attttcaagt tttcacacct ttctcaaaaa
     2941 cgcttgactc tgacctaagg ggaggggtta tactatcatt gtaaggagga aatcatgtac
     3001 catataaaag aagctgcgca gctttcaggt gtctctgtca agaccctgcc ctgcatcact
     3061 atgacaagat aggactcttg gtccccttaa agtcggaaaa cggctatcga acctatagtc
     3121 aagaggattt ggaacgcctt caggtcattc tttactacaa atatctaggc ttttctttag
     3181 agaaaatagc agaactgtta aaggaagaaa ggacagattt attgctccat ttgactaggt
     3241 agttggacta tctaactcgc gaaaggcaac atctggatac cttgatttcc accttgcaaa
     3301 aaactattca agaacaaaaa ggagaaagaa aaatgaccat tgaggaaaaa ttcacgggat
     3361 ttagctatca agacaatcaa aaataccacc aagaagcggt agagaaatat ggtcaagaag
     3421 tcatgggaca agcgctcgaa cgccaaaaag gtcacgaaga cgaggctacg gccgccttta
     3481 accaagtctt tcaaactttg gcacaaaatc ttcaagttgg tttacctgca acagcaaccg
     3541 aaaaccagga gcaagcagcc aagctcttgc aagccattcg cacttatgga tttgactgct
     3601 ctattgaggt attcggtcat atcggtaaag gttacgtcta caacccagag tttaaggaaa
     3661 acattgacaa gtttggttct gaaacagccc agtacacgtc agatgccatt gcggcttacg
     3721 ttcagacaaa tgcagaaaaa ataggctagg aatttcctag cctatttttt acttcaaatc
     3781 ataaagccag tcgtcaccgt ttttgtagta aaagaattca ctgagatctt cttctagaaa
     3841 cacacgaagc atatcagaca tatcatcggt tgcaagtttt agatgagaaa gattttcaaa
     3901 gtcctcccac caaactttcc cttcgtctga agactggagt tcaccagtaa agtgttctgt
     3961 cttgtaaaaa aggacgacat aacgataatc cttgtcgtca taccagtttt tgataccaca
     4021 gagttggggt ttggaaatga tcagaccagt ttcttctttc acttcacgaa tgacagcatc
     4081 gacaaaggat tcgccacgtt caacatgacc accaggaaaa gtaatgccag accagtcggg
     4141 actaactcgg tcttggacca ggaccttatc tccgttttta atcatacaca tgttaacaaa
     4201 ttcgactgcc tctcttctgt tcattcttca caacctttaa tctttaatca taatgcagac
     4261 ttcccgccac ccagccggta cagagggcag aagtgatgtt aaagccaccc gtgtgggcat
     4321 tgatatccat aacttcgcct gtaaagtgga ggccaggtac cagcttactt tcaagggttt
     4381 taggattgat ttccttgaga ctgactccac ccttggtaac aaaggacttt gcaagggaca
     4441 tttttccagt tacaggaatt ttaagttctt taatggactg gacaagttgt tctcgttcct
     4501 tttcagtcag ttgtttgact ttttcaggat atccttgtac aaaaaattcg gccaagcgtt
     4561 ctggtaacaa ggtttttaaa gcgtttttca aggatttttc ccgattttct tctagaaatg
     4621 taaccaagtc cttctcagaa agttgaggca aaacatcgag tgagagaacc tccccacctt
     4681 tgacaaagct agacatgcgt agggcagcag gacctgacaa accaaagtgg gtaaagagta
     4741 aatcatgagt gatgacatgc ttaccataac ttagggtcac atcgtccaga gaaatccctt
     4801 gtaaggcttt atgtggaaaa tctgttaata aaggactttc agcagcctca agatcggtga
     4861 tggtatgctt aaaatggcga gcaatctcgt gaccaaaacc agtcgaacca gtcgaaggat
     4921 aagacttacc acctgttgtg acaatgagtt tctcacaagt gaaggtttga tccgctgact
     4981 taaggacaaa ctggtcatct acttttttaa cagaaacgat ttctatttga gtagcaactt
     5041 gaccacctag ttcggtgatt ttcttttcca aagcttcgat aatagtccga gacttgtcac
     5101 tggctggaaa gacgcgtccg tggtcttcga ccttaagttt aacaccattt tctgtaaaaa
     5161 agttgatgat gtcatgatta tcgaactggg agaaaacact gtaaagaaag cgtccgtttc
     5221 caggaattcc agctagcagg ttgtctaagc taccattgtt ggtcacattg caacgtcccc
     5281 caccagtccc agctaatttt tttccaagtt tccgattttt ttcgatgagg agggttttct
     5341 gtccataaaa gctactggaa atcgtagcca tcataccagc aggtccccca ccgatgacaa
     5401 tagtatcaaa atgtttcata gctctattgt accacaaaaa aacaagagat gatggtcacc
     5461 tcttgtcaag aatgcaatta atcaatttca tagcccatca gcaaaccgcc ctcttctgca
     5521 tagaaactgc agagaccaga ggttggtaga attttaatat ccgcttgtgg gaaggtttca
     5581 cggattcgct ctgagagctg ttgacaacat ttttcgttat tgcgttgggc catgacaata
     5641 cggccaccag catatccagc ttttactaac tcatcatagg cagcttgaac tgatttcttt
     5701 gatccccttg ctttttgtag caattcgaga gttccagttt cactagcttt tccgaccata
     5761 cgaatgttga gaaggccaac gaccgtaccg ataagcttgc tcaaacggcc gttcttcacc
     5821 aagttatcga ctttggctag gacaaagagc aacttagttt tttcttgata ggcagtgata
     5881 gcttcaacca cttcttcaaa agacaagccc tggtcaatca agtcattcaa tttttctacg
     5941 agtaggtcaa cttcaccacc agcagataaa ctatcaatca catgaatctt agtgtcagga
     6001 tggtcttcta gataaatatt ctttgctagt tgagcactat tgtgactgcc agaaagggta
     6061 cctgtgatgg ttactaggaa aatgtttttg gcaccttcaa atgctcgcaa atagtcatct
     6121 gggcttggac aagccgattt tgaagcttct gcagttgcat acatggtttc catcatttgg
     6181 tcaatatcga gactggcgtc atcaacaaag acctgatcag ctacttgaat ggttaagggg
     6241 acacttacaa aggttgtgtt aatagctggt gttggcagtt gacgataatc acaaccagag
     6301 tcagcaataa ttttccaagt catagaaatt ctccatcttt gtcagttata cattgacaaa
     6361 ggttctgtct ttttttacaa ttatatcatg aaaacccttg aaacaaaagc atcatccgtc
     6421 tgttgtgaca agtagaaaga aaatgttatg tctgaacgta gaatctctga aaagtctctt
     6481 gaaaatctca gaaaatcaaa ccaagaatcc aatttattaa ccagagaagc cattgaaaca
     6541 gccctcttgc aactcttgga aaaaaaggag ctgaccaaga ttagtatttc tgaattggtc
     6601 aaacgtgcag gtgtttcgcg tgcggccttt tatcgcaatt atgactccaa agaggaaatt
     6661 ttagagagcg tctttaaacg aactgtccac aatattatgg aacagctgca tcattacgat
     6721 ttaaagacag acctttattt ggtttgggtt caccttttcc gggaggccag aaaggaagct
     6781 agagtgattc aattggcctt ggattaccat ctggaaaaaa tctttgtcca agccatgcag
     6841 gaatttctag aaaaatacca tgggaaatca aaaggtgtca gctcttatct tcattccttc
     6901 tggagctcag ccatcgtctc tgtccttcta aaatggatca aggatggcat gaaggtacca
     6961 gctgaaaaga ttgcagattt aggtttacca ttttttaaaa aatagagaaa aaggagaaaa
     7021 gagatgactg aaaaaagact ggcctgggat gagtattttg cagcccaagc cctactaatt
     7081 gcgaatcgtt ccacttgtaa acgtgctaaa gtgggcgcga ttctggtaaa agataataag
     7141 gttatttcca ctggttacaa tggttcggtg tcagggactg agcattgtat tgatcacgaa
     7201 tgtttggtca ttgaaggcca ctgtgttcgc acccttcacg ctgaggtcaa tgctatcctt
     7261 caaggtgctg agcgtggtgt tcccaaaggc tttacagcct atgtaaccca ttttccttgt
     7321 ctcaactgta caaaacaatt gcttcaggtc ggctgcaagc gcgtggttta tatcaaccag
     7381 taccgaatgg acgactacgc ccaatacctt tatcaagaaa aagggacaga attgacccat
     7441 ttaccacttg agacagtaca gacagctctt aaagaggctg atctaatgta aaaattatca
     7501 aaaataaatg gcttagaaag atttttaaac cgttttttgg tataataaga agaataaatt
     7561 gaaagaagga attccaaaaa tgggaaaaat tgaagttatt aatcacccac tgattcaaca
     7621 caaattgtca atcttgcgtc gtacagatac ttctacaaaa gcttttcgtg agctagtaga
     7681 tgagattgca atgttgatgg ggtatgaagt acttcgtgat cttccactag aagatgtgga
     7741 aatcgaaaca ccaattacaa aaacagttca aaaacaattg gcaggtaaga aattggccat
     7801 cgtcccaatc ttgcgtgcag gtatcgggat ggttgatggt ctcttgagct tggttccagc
     7861 tgctaaagtt ggccacatcg gtatgtaccg tgatgaagaa acacttcaac cagttgaata
     7921 cttggtgaaa ttgcctgagg acattgacca acgtcaaatt tttatagtag acccaatgtt
     7981 ggcaacaggt ggctcagcaa tcttggctgt tgattctctt aaaaaacgtg gcgcatcaaa
     8041 tatcaaattt gtctgccttg tatctgctcc agagggtgta aaagcccttc aagaagctca
     8101 tccagatgta gaaatcttta cagcagcctt ggatgaacgt ttgaacgaac acggttatat
     8161 cgttccaggt cttggagatg ctggagaccg cttgttcggt acaaaataag atcgaaaaga
     8221 agaatgactt ggaaaagtat ttccagtcac gaaaggaggt tgggtttttg tttctgtcta
     8281 atgaaagcag agcaaaaatt tgaccttttt tgaccaagat attataatag tcttatcttc
     8341 agtcatttga ccaacaaaat taaaactcaa aaggagaaat gaatgattcc tgtagttatt
     8401 gaacaaacaa gccgtggaga acgttcttac gatatttact cacgtcttct caaagaccgc
     8461 atcattatgc tgacaggtcc ggttgaagac aatatggcta actctgttat tgcccaattg
     8521 cttttcttgg atgcccaaga tagtacaaaa gatatttacc tttatgtcaa tacaccaggt
     8581 ggttctgttt cagctggttt ggcaatcgta gataccatga actttatcaa ggcagatgtc
     8641 caaaccattg ttatgggaat ggctgcatct atgggaacag tcatcgcatc aagtggagca
     8701 aaaggcaaac gtttcatgct tccaaatgct gaatacatga ttcaccaacc aatgggcggt
     8761 acaggtggtg gtacccaaca aactgatatg gctatcgctg cagaacactt gctcaaaact
     8821 cgtaatacct tggaaaaaat cttggctgaa aattcaggtc agtcaatgga aaaagtccat
     8881 gcagatgcag aacgtgataa ctggatgagc gcccaggaaa cacttgaata tggctttatt
     8941 gatgaaatta tggccaacaa ttcattgaac taatgatgat agaaggcaaa ctcgactggg
     9001 tttgcttttt ttggtataat agggagagat ttcttagaaa gaggatttat catgtttgaa
     9061 aaagtcaatc gctctggctt gattatctat ctttactata atcgtgatgc caaaaaactg
     9121 caggattatg gagatattac ctatcattcc aagaaacatc gttacttaca actctatgtt
     9181 ccaactcaag aagtggagca attggtcgga cgcttgagca aggaaaaatt tattaaaaaa
     9241 gttagggttt gtcatatcca agagttggaa acaccctttg tgggcaatct ttatcgatag
     9301 gaaaacgtta tcatcgaaaa agttcaagga aaacattgac aattttctga taattcggta
     9361 tattcttaac agactattta agaaataagg agacaaaaag atgaagaaaa aatttgccct
     9421 atcgtttgtg gcgcttgcaa gtgtagcact tcttgcagcc tgtggagaag tgaagtctgg
     9481 agcagtcaac actgctggta actcagtaga ggaaaagaca attaaaatcg ggtttaactt
     9541 tgaagaatca ggttctttag ctgcatacgg aacagctgaa caaaaaggtg cccaattggc
     9601 tgttgatgaa atcaatgccg caggtggtat cgatggaaaa caaatcgaag tagtcgataa
     9661 agataataag tctgaaacag ctgaggctgc ttcagttaca actaaccttg taacccaatc
     9721 taaagtatca gcagtcgtag gacctgcaac atctggtgcg actgcagctg cggtagcgaa
     9781 cgctacaaaa gcaggtgttc ccttgatctc accaagtgcg actcaagatg gattgactaa
     9841 aggtcaagat tacctcttta ttggaacttt ccaagatagc ttccaaggaa aaattatctc
     9901 aaactatgtt tctgaaaaat taaatgctaa gaaagttgtt ctttacactg acaatgccag
     9961 tgactatgct aaagggattg ccaaatcttt ccgcgagtca tacaagggtg aaatcgttgc
    10021 agatgaaact ttcgtagcag gtgacacaga cttccaagca gcccttacaa aaatgaaagg
    10081 gaaagacttt gatgctatcg ttgttcctgg ttactataat gaggctggta agattgtaaa
    10141 ccaagcgcgt ggtatgggaa ttgacaaacc aatcgttggt ggtgatggat tcaacggtga
    10201 ggagtttgta caacaagcaa ctgctgaaaa agcatcaaac atctacttta tctcaggctt
    10261 ctcaactact gtagaagttt cagctaaagc taaagccttc cttgacgctt accgtgctaa
    10321 gtacaatgaa gagccttcaa catttgcagc cttggcttat gattcagttc accttgtagc
    10381 aaacgcagca aaaggtgcta aaaattcagg tgaaatcaag gataaccttg ctaaaacaaa
    10441 agattttgaa ggtgtaactg gtcaaacaag cttcgatgca gaccacaaca cagtcaaaac
    10501 tgcttacatg atgaccatga acaatggtaa agttgaagca gcagaagttg taaaaccata
    10561 atagaaaaat gttgaaatag ggaatgagcc tttgactcac tccctgtttc gatatttaat
    10621 actcttcgaa aatctcttca aactgcgtca acgtcgcctt ggattatata tgtgactgac
    10681 ttcgtcagtc ttatctacaa cctcaaagca gtgctttgag caacctgcgg ctagtttcct
    10741 agtttgctct ttgattttca ttgagtataa gaacctatca aaaagtgagg gaaacccctc
    10801 ggaattataa atagaaagag tgaatcttat gctccaacaa ctcgtaaatg gtttgattct
    10861 aggtagtgtt tacgcgctgt tagccctagg atataccatg gtttacggaa ttatcaagct
    10921 catcaacttc gcccatggtg atatttatat gatgggagcc tttatcggtt atttcttgat
    10981 caattctttc caaatgaatt tctttgtagc gcttattgta gctatgctag cgacagctat
    11041 tcttggtgtc gtgattgagt ttcttgctta ccgacctttg cgccactcta ctcgtattgc
    11101 tgttttgatt acggctattg gggtttcttt cctattggag tatggaatgg tctatctggt
    11161 tggtgccaat acccgtgcct tccctcaagc gattcaaaca gttcgatatg atttgggacc
    11221 aattagctta acaaatgtgc agttaatgat tttggctatt tccttgattt tgatgatttt
    11281 gttacaagtc attgtccaaa agactaagat ggggaaagcc atgcgtgcag tatcagtaga
    11341 tagcgacgcg gcgcaattga tggggatcaa tgtaaaccgt acgattagct ttaccttcgc
    11401 tttgggttct gctcttgcgg gtgcggctgg tgttctgatt gctctttatt ataactctct
    11461 tgagcctttg atgggggtta ctccaggtct taaatctttc gttgccgcag tacttggtgg
    11521 tatcggaatt attcctggtg cggctcttgg tggctttgtg attggtctat tggaaacctt
    11581 tgcgactgcc tttgggatgt cagatttccg tgatgccatt gtttatggaa tcttgttgtt
    11641 gatcttgatt gtccgcccag ctggtatcct tggtaagaat gtgaaagaga aggtgtaaac
    11701 gatgaaggaa aatttaaaag ttaatattct atggttactc cttttgttag ctggctatag
    11761 cttgattagt gtactggttt cagtcggagt acttaatcta ttctatgtac agattttaca
    11821 gcaaattgga attaatatta ttttggctgt tggtctcaac ttaatcgttg gtttttcagg
    11881 acaattttca cttggtcatg ctggtttcat ggcgattggt gcctatgcag cagctattat
    11941 tggttctaaa ttaccaacct acggtgcctt ctttggagct atgcttgtag gggctttgct
    12001 ttcaggagca gttgccttac ttgtcggcat tccaaccttg cgcttgaagg gggactatct
    12061 tgcggtagca actctgggtg tttctgaaat tatccgtatc tttatcatca atggtggaag
    12121 ccttacaaat ggtgcggcag gtatcttagg gattcctaac tttacaactt ggcaaatggt
    12181 ttacttcttt gtcgtgatta caaccattgc aaccttgaac ttcttgcgta gcccaattgg
    12241 tcgttcaacc ctctctgttc gtgaagatga aatcgctgct gagtcagttg gggttaatac
    12301 gactaaaatt aaaatcatcg cttttgtctt tggtgccatt actgcaagta ttgctgggtc
    12361 acttcaggca ggatttatcg ggtctgttgt accgaaagat tacaccttca tcaactcaat
    12421 caacgttttg attattgttg tatttggtgg actcggttcc attacaggtg cgattgtttc
    12481 ggctattgtt ctgggaattt tgaatatgct tctccaagat gttgctagtg tgcgtatgat
    12541 tatttacgct ttggccttgg tattggtaat gattttcaga ccaggtggac tccttggaac
    12601 atgggaactg agcctatcac gtttctttaa aaaatctaag aaggaggaac aaaactaatg
    12661 gcattacttg aagtaaaaca gttaaccaaa cattttggtg gtctaacagc tgttggagat
    12721 gtgactcttg aattgaacga aggggaactg gttggattaa tcggtccaaa cggagctggg
    12781 aaaaccaccc ttttcaacct tttgaccggt gtttatgaac caagcgaggg aacagtaacc
    12841 ctagatggtc accttttgaa tgggaaatca ccttataaga ttgcctcttt gggacttgga
    12901 cgtactttcc aaaatatccg tctctttaaa gatttaacag ttttagacaa tgttttgatt
    12961 gcttttggaa accatcacaa acaacatgtt tttactagtt tcttacgctt accagctttt
    13021 tacaagagtg aaaaagaatt aaaggctaaa gctttggaat tgttgaaaat ctttgattta
    13081 gatggtgatg cagagactct tgctaaaaat ctttcctacg gacaacaacg tcgtttggaa
    13141 attgttcgtg cccttgctac ggaacctaaa attctcttct tagatgaacc agcagcaggt
    13201 atgaacccac aggaaacagc cgaattgact gagttaattc gtcgtatcaa agatgaattt
    13261 aagattacaa tcatgttgat tgaacacgat atgaatctgg tcatggaagt aacagaacgt
    13321 atctacgtac ttgaatatgg ccgtttaatt gctcaaggaa ctccagacga aattaagacc
    13381 aataaacgcg ttatcgaagc ttatctagga ggtgaagcct aatgtctatg ttaaaagttg
    13441 aaaatctttc tgtgcattac ggtatgatcc aagcagtccg tgatgtaagc tttgaagtta
    13501 atgaaggaga agttgtttcc cttatcggtg ccaacggtgc aggtaagaca actattcttc
    13561 gcaccttgtc aggtttggtt cgaccaagtt caggaaagat tgaattttta ggtcaagaaa
    13621 tccaaaaaat gccagctcag aaaattgtgg caggtggtct ttcacaagtt ccagaaggac
    13681 gccacgtctt tcctggcttg actgttatgg aaaatcttga aatgggagct ttcttaaaga
    13741 aaaatcgtga agaaaatcaa gctaacttga agaaggtttt ctcacgcttt cctcgtcttg
    13801 aagaacgtaa gaaccaagat gcagctactc tttcaggagg ggaacaacaa atgcttgcca
    13861 tgggacgcgc tcttatgtca acaccaaaac ttcttctttt agatgaacca tcaatgggac
    13921 ttgccccaat cttcatccaa gagatttttg atatcattca agatattcag aagcaaggaa
    13981 caaccgtcct cttgattgaa caaaatgcca ataaagcact tgcaatctct gaccgaggat
    14041 atgtactgga aacagggaaa atcgtcctat caggaacagg aaaagaactc gcctcatcag
    14101 aagaagtcag aaaagcatat ctaggtggct aaaacaatcc agtggattgt tttagtcggc
    14161 agatgaagat tacgaagtaa tcatcattat agtctgggag actagtttag gttgcagata
    14221 aagattgcga agcaatcatc aaatccagtg gattgtttta gtcggcagat ggagattacg
    14281 aagtaatcat caatatagtc cgggggacct ttttagtcgg tagattgaga ttgcaaacaa
    14341 atctgcatct acattgaaag cttaatttct aataattgaa aaaatcgaat gaaaaatttc
    14401 ttaccttcat tcacagagct cgatttcaga gctctttttg ctagcttatt catacttttc
    14461 tgaatttcga aaaagaaatg taagcgtttg atagatttac aaaaagattg tataataggg
    14521 ataagaatag aaaaggagaa gtctcatggc agttaaagat tttatgaccc gcaaggtagt
    14581 ttatattagt ccagatacaa cagtatctca tgcagcagat ttgatgagag agcaaggttt
    14641 gcaccgtctg cctgttatcg aaaatgatca attagttggt ttggtgactg agggaaccat
    14701 tgcacaagca agtccatcta aagcaacaag tctttctatc tatgagatga attatcttct
    14761 gaataagaca aaagtaaaag atgtcatgat tcgcgatgtt gtcactgtct caggctatgc
    14821 tagtctataa gatgcaactt atctgatgtt gaaaaataag attagtattc tccctgtcgt
    14881 agataaccat caagtatacg gagttattac tgaccgtgac gttttccaag cctttcttga
    14941 aattgcaggt tatggcgaag aagggattcg tgtacgcttt gttacagaag atgaagttgg
    15001 tgttcttgga aaaattgttt ctttgattgt agaagaaaat ttgaatatct cccatacagt
    15061 caatattccg cgtaaggatg gtaaggtgat tatcgaagtg caaatcgatg gatcaattga
    15121 tttaccagcc ttgaaagaaa aatttgaagc aaatggtatt caagtggaag aaatcgctcg
    15181 cacttcagca aaagtcttgt aagaagggaa gcccaaaggc ttcttttttc atgaaaaggg
    15241 gattagagaa aaagatggaa agaaatgata aaatatgcta taatgaaata atgtaaaaaa
    15301 ggagtattta tggacatttc agtaattcgt cagaaaattg acgcaaatcg tgaaaaatta
    15361 gcttctttca gggggtctct ttgacctcga agggctagag gaggagattg ccatcttgga
    15421 aaacaagatg acagaacctg atttttggaa cgataatatt gcagcccaaa aaacgtcgca
    15481 agaattaaat gaattaaaaa acacttacaa taccttccat aagatggaag agttgcagga
    15541 tgaagtcgaa attttattgg attttttggc tgaagacgag tcagtgcatg atgaactggt
    15601 agcgcagtta gccgaacttg ataagataat gaccagctac gagatgactc tactcttgtc
    15661 agaaccttat gaccacaaca atgccatctt ggaaatccat ccaggttctg gtggtactga
    15721 ggcgcaggac tggggtgata tgttgcttcg tatgtatact cgttatggta atgctaaagg
    15781 ctttaaagtg gaagtgttgg attaccaagc aggtgatgag gctggtatta agtcggtaac
    15841 tttatcattt gaagggccta atgcctatgg tctcctcaag tcagaaatgg gtgttcaccg
    15901 cttagtgcga atctcaccat ttgactctgc caaacgtcgc catacctctt tcacatctgt
    15961 agaagtgatg ccagaattgg atgatactat tgaagtggaa atccgtgaag atgatatcaa
    16021 gatggatacc ttccgttcag gtggtgccgg tggacaaaac gtcaataagg tttcaacagg
    16081 tgtacgttta acccacattc caactggaat tgttgtccaa tcaacagtgg atcgtaccca
    16141 gtatggaaat agagatcgtg ccatgaagat gttgcaggct aagctctatc aaatggagca
    16201 agagaagaag gctgcagagg tagattctct caaaggtgag aaaaaggaga tcacttgggg
    16261 aagccaaatc cgttcttatg tcttcacgcc ttatactatg gtaaaagatc accgaactag
    16321 ctttgaggtt gctcaggtag ataaggttat ggatggggac ttagatggtt ttatcgatgc
    16381 ttatctcaag tggcgaatta gctaagatag aaaggaactc acatgtcaat tattgaaatg
    16441 agagatgtcg ttaaaaaata cgacaacgga acgactgctc tacgcggtgt ttcggttagc
    16501 gttcaaccgg gggaatttgc ttacatcgta ggaccttcag gagcagggaa gtcaactttt
    16561 attcgttctc tgtatcgtga agtaaaaatc gataaaggaa gcctatcagt tgctggtttt
    16621 aatctggtta agatcaaaaa gaaagatatc ccgcttctac gtcgtagtgt tggggttgtc
    16681 ttccaggatt ataaattgtt accaaagaaa actgtctatg aaaatattgc ttacgctatg
    16741 gaagtaatcg gggaaaatcg ccgtaatatc aaaagacgag tgatggaagt tttggacttg
    16801 gttggattga agcataaggt tcgttctttc ccaaatgaac tctcaggtgg ggagcaacag
    16861 cggattgcga ttgcgcgtgc aattgtaaat aatcccaaag tattgatagc tgatgagcca
    16921 acaggaaatc tggatccgga taattcatgg gaaattatga atcttttgga acggattaac
    16981 ctacaaggaa caactatttt gatggcgact cataatagcc agattgtaaa taccttgcgc
    17041 caccgtgtca ttgccattga aaatggccgt gtcgttcgtg acgaatcaaa aggagagtat
    17101 ggatacgatg attagtagat tttttcgcca tttatttgaa gccttaaaaa gtttgaaacg
    17161 aaatggttgg atgacagtag ctgctgtcag ttcagtcatg attactttga ccttggtggc
    17221 aatatttgca tctgttattt tcaatacagc gaaactagct acagatattg aaaataatgt
    17281 ccgtgtagta gtttatatcc gaaaggatgt ggaagataat agtcagacaa ttgaaaaaga
    17341 aggtcaaact gttacaaata atgactacca caaggtatat gattctttga agaacatgtc
    17401 tacggttaaa agtgttacct tttcaagtaa agaagaacaa tatgaaaaat taaccgagat
    17461 aatgggagat aactggaaaa tctttgaagg agatgccaat cctctctatg atgcctatat
    17521 tgtagaggca aacactccaa atgatgtaaa aactatagcc gaagatgcta aaaaaattga
    17581 aggtgtctct gaggttcaag atggcggtgc caatacagaa agactcttca agttagcttc
    17641 atttatccgt gtttggggac tagggattgc tgctttgtta atttttatcg cagttttctt
    17701 gatttcaaat accattcgta ttaccattat ttcccgcagt cgcgaaattc aaatcatgcg
    17761 cttggtcgga gctaaaaaca gttatatccg tggaccgttc ttgttagaag gagcctttat
    17821 cggtttattg ggagctatcg caccatctgt tttggtcttt attgtttatc aaattgttta
    17881 ccaatctgtc aacaaatcgt tggtagggca aaatctatcc atgattagtc cagatttatt
    17941 tagtccgttg atgattgccc tactatttgt gattggggtt ttcattggtt cattgggatc
    18001 aggaatatcc atgcgccgat tcttgaaggt ttaggtaaaa tagctgcttt tatgaggaga
    18061 ttgtaaaatc tccttttttg ctacaagagt ttttgaaaag agatgcgcag aagaaaagag
    18121 cttccaaaga agtcccccag agaagacttc ttgatggcta atttgaactt gaaatttggc
    18181 gcactagaaa ttttttagaa aaaagtgtaa tttttttata aaagccttta caaaaaaaat
    18241 taaagtggta taattaaatc atagaaagtg caaacgtttt cgtaaaacgt ttgcctaaat
    18301 aaaaataaag gagatttgaa tgatgaaaga tacattcaaa aatgtcttgt ctttcgaatt
    18361 ttggcaaaaa ttcggtaagg ctttgatggt agttatcgcg gttatgccgg ctgctggttt
    18421 gatgatttca atcggtaagt ctatcgtgat gattaaccca acctttgcac cacttgtcat
    18481 cacaggtgga attcttgagc aaatcggttg gggggttatc ggtaaccttc acattttgtt
    18541 tgccctagcc attggaggaa gctgggctaa agaacgtgct ggtggtgctt tcgccgctgg
    18601 tcttgccttc atcttgatta accgtatcac tggtacaatc tttggtgtat caggcgatat
    18661 gttgaaaaat ccagatgcta tggtaactac tttctttggt ggttcaatca aagttgctga
    18721 ttactttatc agtgttcttg aagctccagc cttgaacatg ggggtattcg tagggattat
    18781 ctcaggtttt gtaggggcaa ctgcttacaa caaatactac aacttccgta aacttcctga
    18841 tgcactttca ttcttcaacg ggaaacgttt cgtaccattt gtagttattc ttcgttcagc
    18901 aatcgctgca attctacttg ctgctttctg gccagtagtt caaacaggta tcaataactt
    18961 cggtatctgg attgccaact cacaagaaac tgctccaatt cttgcaccat tcttgtatgg
    19021 tactttggaa cgtttgctct tgccatttgg tcttcaccac atgttgacta tcccaatgaa
    19081 ctacacagct cttggtggta cttatgacat tttaactggt gcagctaaag gtactcaagt
    19141 attcggtcaa gacccactat ggcttgcatg ggtaacagac cttgtaaacc ttaaaggtac
    19201 tgatgctagt caatatcaac acttgttaga tacagtacat ccagctcgtt tcaaagttgg
    19261 acaaatgatc ggttcattcg gtatcttgat gggtgtgatt gttgctatct accgtaatgt
    19321 tgatgctgac aagaaacata aatacaaagg tatgatgatt gcaacagctc ttgcaacatt
    19381 cttgacaggg gttactgaac caatcgaata catgttcatg ttcatcgcaa cacctatgta
    19441 tcttgtttac tcacttgttc aaggtgctgc cttcgctatg gctgacgtcg taaacctacg
    19501 tatgcactca ttcggttcaa tcgagttctt gactcgtaca cctattgcaa tcagtgctgg
    19561 tattggtatg gatatcgtta acttcgtttg ggtaactgtt ctctttgctg taatcatgta
    19621 ctttatcgca aacttcatga ttcaaaaatt caactacgca actccagggc gcaacggaaa
    19681 ctacgaaact gctgaaggtt cagaagaaac cagcagcgaa gtgaaagttg cagcaggctc
    19741 tcaagctgta aacattatca accttcttgg tggacgtgta aacatcgttg atgttgatgc
    19801 atgtatgact cgtcttcgtg taactgttaa agatgcagat aaagtaggaa atgcagagca
    19861 atggaaagca gaaggagcta tgggtcttgt gatgaaagga caaggggttc aagctatcta
    19921 cggtccaaaa gctgacattt tgaaatctga tatccaagat atccttgatt caggtgaaat
    19981 cattcctgaa actcttccaa gccaaatgac tgaagcacaa caaaacactg ttcacttcaa
    20041 agatcttact gaggaagttt actcagtagc agacggtcaa gttgttgctt tggaacaagt
    20101 aaaggatcca gtatttgctc aaaaaatgat gggtgatgga tttgcagtag aacctgcaaa
    20161 tggaaacatt gtatctccag tttcaggtac tgtgtcaagc atcttcccaa caaaacatgc
    20221 ttttggtatt gtgacggaag caggtcttga agtattggtt cacattggtt tggacacagt
    20281 aagtcttgaa ggtaaaccat ttacagttca tgttgctgaa ggacaaaaag ttgcagcagg
    20341 agatctcctt gtcacagctg acttggatgc tatccgtgca gcaggacgtg aaacttcaac
    20401 agtagttgtc ttcacaaatg gtgatgcaat taaatcagtt aaattagaaa aaacaggttc
    20461 tcttgcagct aaaacagcag ttgctaaagt agaattgtaa tatacttgag gttggaagct
    20521 gtattccaac ctcttatttt gggagaaaag aatgaaattt ttaacactca atactcacag
    20581 ttggatggag aaagaagcag aggaaaaatt ccagattttg cttgaagata ttcttgaaaa
    20641 ggactatgat ttgatttgtt tccaagaaat caatcaggag atgacctcgt cagaggtgga
    20701 ggttaatgac ctttatcaag ctttgccagc agctgagcct attcaccaag accattatgt
    20761 tagactcttg gttgaaaagt tgttagagca agggaaaaat tactactgga cctgggctta
    20821 taaccatatc ggctatgacc gctaccacga aggtgtggct atcttgtcta aaacacctat
    20881 tgaagccaga gaaattttgg tttcagatgt ggatgatcca acagactatc atactcgccg
    20941 tgttgcccta gctgaaactg tagtcgatgg caaggagcta gcagttgcca gtgttcatct
    21001 ctcttggtgg gataaaggtt tccaagaaga atgggcacga tttgaggctg tcttgaaaaa
    21061 attgaacaag ccacttttac tagctggaga ttttaacaac ccagcaggtc aggaaggtta
    21121 ccaagctatt ttagctagtc cattaggctt acaagacgca tttgaagttg ctcaagagaa
    21181 aagtggtagc tatactgttc cgcctgaaat tgatggctgg aaagggaaca ctgaacccct
    21241 tcgaatcgat tatgtcttta ctaccaaaga gttagcggtg gaaaatttac atgtcgtatt
    21301 tgatggtaac aagagtccac aagtgagtga tcactatggc ttgaatgcta tattaaactg
    21361 gaaataataa ctgaaaagag gttggaacta taaaattcca gccttttctt actagagaag
    21421 ctactggaaa tagcctaaat aagtgagact actgtaatgg aataaaatat ggtataattg
    21481 ataaggtaga tagaatcgag gatgttatgt catttacgaa atttcaattt aaaaactata
    21541 ttagagaagc cttgaaggag ttaaaattta caactccaac agaggtgcaa gacaagttga
    21601 ttcctattgt tttggcaggt cgtgacctag taggagaatc aaaaacaggt tcaggtaaga
    21661 ctcatacttt cttgttaccg attttccagc aattagatga agctagcgat agtgtacaag
    21721 cagtgattac tgcaccgagt cgtgagttgg ctactcaaat ttaccaagta gcgcgtcaga
    21781 tttcagctca ctcagatgtc gaagttcgtg tggttaatta tgtgggtggt acggataagg
    21841 ctcgccagat tgagaaattg gcaagcaatc agcctcatat tgttattgga acaccaggcc
    21901 gtatctacga cttggttaaa tctggtgatt tagctattca taaagccaag acatttgttg
    21961 ttgatgaagc agatatgacc ttggatatgg gattcttgga aactgttgat aagattgctg
    22021 gcagtcttcc aaaagacttg caattcatgg tcttctcagc gactatccca caaaaactgc
    22081 aaccattctt gaaaaaatac ttatcaaatc ctgttatgga gaaaattaag accaaaacgg
    22141 ttatttctga caccattgat aattggttga tttcgaccaa gggacatgat aagaatgctc
    22201 aaatttacca gttgactcag ttgatgcagc cgtatttggc aatgattttt gttaacacta
    22261 aaacacgtgc tgatgaattg cattcatatc tgactgctca aggcttgaag gttgcaaaaa
    22321 tccatggaga tattgcccct cgtgaacgca agcgaatcat gaatcaggtg caaaatctgg
    22381 attttgagta tattgtcgca acagatttgg cagcgcgtgg gattgacatt gaaggtgtca
    22441 gccatgtcat caatgatgcc attccgcaag acttatcttt ttttgttcat cgtgttggtc
    22501 gtactggacg aaatggccta ccaggtacag ctattaccct ttatcagcca agtgatgact
    22561 cggatatccg tgagttggag aaattgggaa tcaagtttag tcctaagatg gtcaaagacg
    22621 gggaatttca agatacctat gaccgtgatc gtcgtgccaa ccgtgagaaa aaacaagata
    22681 aacttgatat cgaaatgatt ggtttggtta aaaagaaaaa gaaaaaagtc aaaccgggtt
    22741 ataagaagaa aattcaatgg gcggttgatg aaaagcgccg taaaaccaag cgtgctgaaa
    22801 atcgcgctcg cggtcgtgca gagcgtaaag ctaaacgcca aacattttaa tagaaattgt
    22861 tggagtattg agctccaact tttttattta tgagaacgaa ctatctaaac cgaaacacta
    22921 cattaaagac tgcaaattgc gattaaaaat ggtataatga taaagttata tagtcccgat
    22981 aagatggtag gtatttatta cgaagagttt tcctatcagt actttgtaac tctataacaa
    23041 tattttttaa gggggggaca tttttatgtc agagcgtaaa ttattcacgt ctgaatctgt
    23101 atctgagggg catccggata agattgcaga ccaaatttca gatgcgattt tggatgctat
    23161 tttagcaaag gatccagagg cgcacgttgc tgctgaaaca gctgtatata ctggttctgt
    23221 ccacgttttt ggtgaaattt ctacaaatgc ctatgtggat attaaccgtg tggttcgtga
    23281 taccattgca gagattggtt ataccaatac agaatatgga ttttctgctg agacggtggg
    23341 agtacaccca tctttggtgg aacaatctcc tgacatcgct caaggtgtta acgaagcctt
    23401 ggaggttcgt ggaaatgctg atcaagatcc actggacttg attggagcag gtgaccaagg
    23461 gctcatgttt ggatttgcag tagatgaaac agaagagctc atgccattgc caattgcact
    23521 cagtcataaa ttggttcgtc gtctggcaga acttcgtaag tctgaagaaa ttagctatct
    23581 ccgtccagat gcaaaatcac aagttacagt tgagtacgat gaaaatgacc gtccggtacg
    23641 tgtagataca gtcgttattt ctactcagca tgatccagag gccactaatg aacaaatcca
    23701 tcaagatgtg attaaaaagg tcatcaaaga agttattcca tcttcttatc ttgatgataa
    23761 gacaaaattc tttatcaatc cgacaggtcg ttttgtaatc ggtggtcctc aaggggactc
    23821 aggtttgact ggtcgtaaga ttattgtaga tacttatggt ggctactctc gtcatggtgg
    23881 tggtgccttc tctggtaaag atgcgactaa ggtggatcgt tcagcctctt atgcggctcg
    23941 ctatattgcc aagaatatcg ttgcagcaga ccttgctaag aaggcagaag tgcagttggc
    24001 ctatgctatc ggtgttgcgc aacctgtttc tgttcgtatc gatactttcg gtacaggaac
    24061 agtagctgaa agtcaacttg aaaaagcggc tcgtcaaatc tttgaccttc gccctgcagg
    24121 gattatccaa atgctggacc tcaagcgtcc aatttaccgt caaacatcgg cttacggtca
    24181 catgggacgt acagatattg atcttccatg ggaacgtttg gataaggtag atgctttgaa
    24241 agaagcagta aaataagatt ttaagagggg aacgtcctct cttttttata gtttttaact
    24301 atactgggat actgttctga aaatccattt tgtgaaagta gagatttaca tgtatagtag
    24361 attgaaacta gaatagtaca cctcaacttc taaaacattg ttagaaatcg atttgactgt
    24421 cctgattgat ttctcctgtt cttgtttcat tttactatat ttctttaaaa atgataaagg
    24481 ttgagatttc tcctcgtaat agataatcat ggggatattt caatccaaag ttttattcgt
    24541 tatcacttga ctattgcaag gttttctaga gcaacagagt catggaatgg actcatggtt
    24601 gagatttctc cttgttgctt ggacttcatt caaaagtctg ttacccaagc cttgttcaaa
    24661 cttccaatac actagctgtt tccatagcat gacttctgta ctagactttc ttttccgaat
    24721 aaatagatag aaccacagaa tctagtaaac ctagaattaa aattatggta taatattagc
    24781 aataaaagaa atctggagga ttagaatcat ggtatcaacg aaaacacaaa ttgctggttt
    24841 tgagtttgac aattgcttga tgaatgcagc aggtgtggct tgtatgacga tagaggagtt
    24901 agaagaggtc aaaaactcag cggcaggaac ctttgttact aagacagcga ccttggactt
    24961 ccgtcagggg aatcctgagc cacgctacca agatgttcca cttggttcca tcaactctat
    25021 gggcttgcca aataatggct tagactatta tttggattat cttttagatt tgcaggaaaa
    25081 agagtcgaac cgaactttct tcttatctct ggtcggcatg tctccagagg aaacccatac
    25141 tattttgaaa aaagtccaag agagtgattt tcgtggtctg actgagctaa atctttcctg
    25201 tccaaatgtt ccaggtaaac ctcagattgc ctatgatttt gagacaacag accggatttt
    25261 ggcagaagtg tttgcttact tcaccaaacc tcttggaatt aaattgccac cttattttga
    25321 tattgttcac tttgatcaag cggcagctat tttcaacaaa tatccgctca agtttgtcaa
    25381 ctgcgttaac tctatcggaa acggcctcta tatagaagac gaatctgtcg ttattcggcc
    25441 taagaatggt tttggtggaa ttggtggaga atacatcaaa ccgactgctc tagccaatgt
    25501 tcacgccttt tatcaacgtt taaatcctca aatccaaatt atcggaacag gtggcgttct
    25561 gactggtcga gatgcctttg aacacatcct ctgtggagca agtatggtgc aggtgggaac
    25621 gacccttcac aaagaaggtg tcagtgcttt tgaccgcatt accaatgaac tgaaagcaat
    25681 catggtggaa aaaggctacg agagcttaga agatttccgt gggaaattgc gctatattga
    25741 ctaaattaaa tcgaaaaatc tgaagaaagg agagacgatg ctagccattg aagaaagtca
    25801 gaagttgact ttatcaaatt taccgagcct gagcctattt acagggacag atcagggtca
    25861 gtttgaagtg atgaagagtc aagtgttgaa acagattggg tatgattctg ctgacctcaa
    25921 ctttgcctac tttgatatga aagaagtagt ttacaaggat gtggaactgg agttggtcag
    25981 ccttcctttc tttgcggatg agaaaatcgt gatattagat tattttatgg atatcacgac
    26041 tgctaagaaa cgctttttga cagatgatga gcttaagtca tttgaggaat accttgacaa
    26101 tccttctcca acaaccaagt tgataatctt tgcagaagga aagctggata gcaaaagacg
    26161 gttagtcaaa ttacttaagc gtgatgccaa ggtcttcgat gcagtagaag taaaagaaca
    26221 agaattgcgc cagtatttcc aaaagtggag tcagaaacaa ggtctgcagt ttaccaatca
    26281 ttcttttgaa aatctcctca tcaagtcggg gtttcaattt agcgaaatcc agaaaaatct
    26341 tctcttttta cagtcctata aggcgaattc tgttatcgag gaagaggata ttgttaacgc
    26401 aattcccaag accttgcagg acaatatttt tgatttaact cagtttattc tgactaaaaa
    26461 gatggatcag gcgcgcgatt tggtgagaga cttgaccttg caaggggaag atgaaatcaa
    26521 actgattgca gtcatgctgg gacaatttcg gacttttact caggtgaaga ttttggcgga
    26581 gtctggccaa acagaatcgc agattgcaag tagtttaggt agttatctgg gacgtaaccc
    26641 aaatccttat caaatcaagt ttgcattaag agattcgaga ggactttctt tgagctttct
    26701 gaagcaagct atttcctatt tgattgagac agactatcag attaagacag gtctttatga
    26761 aaaaggtttc ctttttgaaa aggcactctt acagattgct agtcaggtca attgacattt
    26821 gttgaaacta caacccacgt caggattatc ttgtcgtggg tttcttgctg atttgcttat
    26881 tttattagaa agtatgtcag tgatttcctt atctataagc aaaaaacttg aaaaaagtga
    26941 atgtttgcgt tatcattttc atatagaaag aaaaagaggt attacagatg gctattatct
    27001 taccagaact tccatatgca tacgacgctt tggaaccata catcgatgcg gaaacaatgc
    27061 atttgcacca tgacaaacac catcaaactt atgtcaacaa tgccaatgca gctttagaaa
    27121 aacaccctga aatcggtgaa gaccttgaag ccttgcttgc tgatgtagaa tctatcccag
    27181 ctgatatccg tcaagcactt atcaacaatg gtggcggaca cttgaaccac gctcttttct
    27241 gggaattgat gactcccgag aaaacagctc cttcagcaga actgacagca gcaatcgatg
    27301 caacatttgg ttcatttgaa gaattccaag cagccttcac tgcagcagca acaactcgtt
    27361 ttggttcagg ttgggcatgg ttggttgtca acaaagaagg gaaacttgaa gtgacttcaa
    27421 cagcaaacca agacacacca atctcagaag gtaaaaaacc aatcttgggc ttggacgttt
    27481 gggaacatgc ttactacgtg aaataccgca acgtgcgtcc tgactatatc aaagctttct
    27541 tttcagtaat caactggaat aaagtagatg aattgtacgc agctgctaaa taatgatagt
    27601 tggagggaag aattgttctt ctctttttta ggttataaga ttctggtctg acaaaatcgt
    27661 cagacttttt tcatttttat gagaaacgtg ctaactgcta gaaattatgg tagaataaag
    27721 tattaagtaa tcgtggaaaa aggatatcta tgcgaaaaga aattgcacct gaattataca
    27781 actataacaa gtttcctggt ccggagttcc atttacacgg ggacaaggtc gaaacggaag
    27841 ggatagcttt ttccttggtt gaaaatatca aggatgcctt tgatgtgacg acttttaatc
    27901 agcgtttttc agaagtatta accaagtttg attatatcgt gggggactgg agcaacgaac
    27961 agcttcgcct acgaggtttt tacaaggatg accgaacaga agaaaaactt gaaaaaatca
    28021 gtcgtttaca agactacctt ttagagtatt gtagttatgg ttgtgcctat tttgtcttag
    28081 aaaatgaagc ccctaagcga gcatcatttg acaagaaaat gcgtaagacg gaagaagaaa
    28141 caccttctag aaaaggaaag aaaccgactc aaactaaacg aaagtcgaat gcagataaga
    28201 aaaatagacg tcgtcagaaa gaccaacatt ctcagaaaga ggacaaggaa caacgtcatt
    28261 ttgtcattcg tcagaagtga gtagagaata aggagaagaa atgaaaccct caattcatag
    28321 tttggcacat caaaccatgc aggaatgggt attggagcag ggagaaaaga aattccgtgc
    28381 agatcaaatc tgggaatggc tctaccgtaa acgtgtgcag tcatttgaag aaatgaccaa
    28441 cctttccaag gatttgattg ctaagctcaa tgaccagttt gtggtcaatc ccttgaaaca
    28501 acgtattgtg caagagtctg ctgatggtac agtcaaatat ctctttgaat tgcccgacgg
    28561 tatgttaatt gagactgtac tcatgcgtca acactatggt ttatctgtct gtgtgaccac
    28621 tcaggtcagc tgtaatatcg gttgtacctt ctgtgcctct ggtttgatta agaagcagcg
    28681 tgacctcaat aacggtgaaa tcgtagcgca aatcatgctg gttcagaaat actttgctga
    28741 tgaaggggtt caggtcaatc ttgccgtgtc tcttcacgca cccaacaatg aacttcgttc
    28801 aagtatcatg aaaattaacc gtgcctttcc gattgaaaaa ctctttgctg ctattgaata
    28861 ctacattgag acaacaaacc gtcgtgtaac ctttgaatat atcatgctta atgaagtcaa
    28921 cgacggtgta gaacaagcct tggaattggc tgaattgctc aagaacatca agaaattgtc
    28981 ttatgtaaac ttgattcctt ataacccagt tagtgagcat gaccaatata gccgtagtcc
    29041 caaagagcgc gtgttggctt tctatgatac gcttaagaaa aaagggggca actgtgtggt
    29101 tcgtcaagag catggtacag atattgacgc agcttgtgga caattgcgct ttaacacaat
    29161 gaaacgtgac cgccagaagg cagtcgcagc ggtaaatcct taatgatgaa gaagacttat
    29221 aatcatattt tggtctgggg agtcattttc tatagcattt gcattgtcta tttttgcttt
    29281 actcctcaag aacaatctac cgtgggagtg ggaactccag atattcagcc tcttggacgc
    29341 ctggtttttc ttttgactcc tttcaattct ctctggaaac tgggcgaagt gagtgacatt
    29401 ggacaattat gttggatttt tttacaaaat atcctcaatg tcttcttgtt ttttcctctg
    29461 attttccaac tcctttatct atttccaaat ttgcggaaaa caaaaaaggt ccttcttttt
    29521 agttttcttg tgagtcttgg aatcgagtgt acgcaattaa tcttggactt tttctttgat
    29581 tttaatcgcg tctttgagat tgatgatttg tggaccaaca ctttgggtgg ctatctggct
    29641 tggctccttt ataaacgatt acataaaaac aagataagga attaaaatga gtattttaga
    29701 agttaaaaat ctgagtcacg gttttggtga ccgtgcaatt tttgaagatg tgtccttccg
    29761 tctcctcaag ggagaacata tcggcctggt cggtgccaat ggtgaaggaa aatcaacctt
    29821 tatgagtatc gtgactggta aaatgctgcc agatgaagga aaggttgagt ggtccaaata
    29881 tgtgacggct ggttacttgg atcagcactc tgtccttgct gaaagacagt cggtgcgtga
    29941 tgttctccgt acggcttttg atgagctttt caaagctgaa gctcgtatca atgacctcta
    30001 tatggaaatg gctgaagacg gtgcggatgt tgatgctctc atggaagaag tgggagaact
    30061 tcaagaccgt ctggagagtc gtgatttcta taccttggat gctaagattg acgaagtagc
    30121 gcgtgctctt ggtattatgg actttggcat ggatacggat gtaacttctt tgtcaggtgg
    30181 gcaaagaacc aaggtgcttt tggcaaaact tctccttgaa aagcctgata ttttgctgtt
    30241 ggacgagccg accaactact tggatgctga gcatattgat tggctcaagc gctatctcca
    30301 aaactatgag aatgcctttg tcctcatttc gcacgatatt ccattcctca atgacgttat
    30361 taatattgtc tatcatgtgg aaaatcaaca gctgacgcgt tactctggtg actactacca
    30421 gttccaagaa gtttatgcta tgaagaaatc tcagctagag gcagcctacg aacgccagca
    30481 gaaagagatt gcagacctca aggactttgt ggctcgtaat aaagcccgtg ttgcaactcg
    30541 taatatggct atgtctcgtc aaaagaaatt ggataagatg gatattatcg aactccaaag
    30601 tgagaaacca aaaccatcct ttgatttcaa accagctcgt acaccagggc gctttatctt
    30661 ccaagccaag aacttgcaaa ttggttacga ccgtcctctt actaagcctt taaatcttac
    30721 cttcgaacgc aatcaaaagg ttgcgattat tggtgctaat ggtattggaa aaacaactct
    30781 cttgaagagt ctcttaggca ttatctcgcc aatcgctggg gaagtggagc gtggagatta
    30841 tttagaactt ggttattttg agcaggaagt agaaggcggt aatcgccaaa ctcctcttga
    30901 agctgtctgg aatgcctttc ctgcccttaa tcaagcagaa gtccgtgcag cccttgcccg
    30961 ttgtggtttg acaaccaaac atattgaaag ccagattcaa gtattatcag ggggagagca
    31021 agccaaggtt cgtttctgtc tcttgatgaa tcgtgaaaac aacgttttag tgctggacga
    31081 gccgaccaac catttggatg tggatgcaaa ggatgagctc aaacgcgctc tcaaagaata
    31141 taggggatct atccttatgg tctgccacga gccagacttt tatgaaggct ggatagacca
    31201 aatatgggat tttaataatt taacttaaga atgagagtaa aagaaataca gtaccgaatg
    31261 tggatactgt atttttttgg tttttaagat ttaaaatcgt agtctttcac cacttcgatg
    31321 ctgtcgattg tgatagcagt agttggcttg tctttttcat ctttttcggc cttagcaatc
    31381 ttatccacaa catccatacc gtcaatcact tgaccaaaga ctgggtgttt gccatctaga
    31441 ctagggtttc caccttcttt gtaggcttcg ataattttct gtggatactt gcttgtaggg
    31501 agtttagaag aggtatctgt agagttttgg ttgatgaaga actggctgcc attggtgttt
    31561 ggttgaccag tattagccat agcaagagca ccacggatgt tatacaaata aggagtaatc
    31621 tcgttcttga aaccagttcc tttgtcttta gtcttatcct tgtcatgcca gatggactga
    31681 ccacctgtac cgtccccttt tggatctcca gtttggacca taaagccatc gatgacacgg
    31741 tggaaggtaa taccgttata gtagccttct ttggcgtgag tgaggaaatt ttcaaccgct
    31801 agaggagcga gtttagggaa gagtttaatg cgaatatcac cttggcttgt gtggagaatc
    31861 acttcggctt catcttcagc aacttcctta gatagttgtg ggaaattggc gttttcgttt
    31921 gttaaagcat cgtttaactc cttggcagat tgggcagcta ctttggaact ttcttcagca
    31981 gcaagactgg aatcaacata atcatcacca cgcagactgc gttggacgct gctacaccca
    32041 gctagggcta cagtagacag taaaagaagg gttgctagtt ttttcattct gatcctctca
    32101 aaaattcatt tataaccatt gtaccctaaa aggaagtgga tttcaaatat tagtagttta
    32161 cagaactggc ttagataata gttgggattt catcatcatc ttgaaaatct tcaacttctt
    32221 ccccagtaaa tggtttctga aaagaactta gtataaaacg ggcaattggt tcaacaatta
    32281 ggaaatttag aggaagagct gcaataaagt ttacgaacca ggcgcttaga taggtattgg
    32341 gtgtgatagc aatgccattg taaaaaaagt ccatagaagg acatgaaagt gaccagctaa
    32401 ttcttcttct gaaaaatgct catgaagggc ttgatccagg tcgcaagatt tcattttaga
    32461 aagtccagta actcccttct tcttcaggat attctttttc atggtagtat ttagatggtc
    32521 aagtgagtca aaagaagttt cgacaagagc ataccctttt tctaccagag tttttagatg
    32581 tttaggggca tcaataccat aagtgtactc aaagtatttt ggaaaccaga tcttaagaaa
    32641 gctcgtaaag ctagccagtt ctctaaacgc taagaactag ctacaacaca gcatttacaa
    32701 cacttcatgg ttcacaccat ggggtgtttt ttaatgattt taagcaaaat tcacacttaa
    32761 ttgaaagtta tttaaattag atgaaaattt attttttgaa aaagtgggaa aacattgatt
    32821 ctaagcgaat tttgaatcat attcgaatgg gagtttttaa aatcatgttt cagtggtaaa
    32881 agtaccaaac tggattctcc atagtaaaat gatatctcct gctagtgaat catctactaa
    32941 taagtccata tttcgcttgg gaacgggctt gggacttaga agccaagtat ccaactctcg
    33001 ttcaggcgat atgaagggtt tgacttggtg ttctttataa aatttttcta aaatagtatt
    33061 cacggatagc atctttcaaa catttatttc ctattctaac aaatttctaa atactttaag
    33121 aaatttatag tctgtcaagt ttttttcttg acacatgtca gaaatctgct ataatagaac
    33181 atgtgctaaa tagctcagct atttcaccga aataaaaaat taaagaaatg agactttaaa
    33241 aatggcagtt aaaatccgtt tgactcgtat gggttctaag aaaaaacctt tctaccgtat
    33301 caacgtagca gattcacgtt caccacgtga cggacgtttc atcgaaacag ttggaactta
    33361 caacccactt gttgctgaaa accaagtaac tttgaaagaa gaccgcgttc ttgcatggtt
    33421 ggctaatgga gctcaacctt cagacacagt acgtaacatc ctttcaaaag aaggcgtatt
    33481 gaaaaaattc cacgattcta aattttcaaa ataagtttaa agtaggttga cagatggata
    33541 cgattgaaaa tctcattatt gcgattgtga aacccttaat ttcacaacca gatgccttaa
    33601 ctatcaagat tgaggataca ccagaatttt tggaatatca tttgaatctt gatcaaagcg
    33661 atgtgggtcg tgtaatcggt cgtaagggtc gcactatttc tgcgataaga acgattgtct
    33721 actctgtccc aactgaatac aaaaaagtaa gaatcgttat tgacgaaaaa taagaagggc
    33781 gggacggatg tctcgctttt ttgtgtggaa tagaagatat agtaaaatga aacaagaaca
    33841 ggacaaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaag taaaggtgta
    33901 ctattctagt ttcaatatac tataaaagat tgaacgttta ggcaattcca agactactta
    33961 atcgaacatt aacagcagtc tcggactgat gaaggtctct ttatgaagtt agtggaggaa
    34021 gtcggaaaag tagccgaggt tttgaatagg cgctctggtc gaaaagaagg gataggattc
    34081 gaacgaggaa cttgccagag aactggctga tatcatttac tacaccgtcg caatcgtagc
    34141 tattaatcat attgacctta ccaagactgt ctttgagaaa gacaaaaccg cagccgttaa
    34201 ttaccaacat aaacataatt tggaaggatt attgaaggga aagtagagat gttgtcccct
    34261 tcctccaact tgagaaaatc cttggaacgt gataaaataa aggataatga tttttaataa
    34321 ttcattatat agaatgagtg gatttcttta tgaagaggtt tgaagtatct acagaaattg
    34381 gtagcctctc tgttacttat caaaagcaaa agaaagtgct agtttgtcta aacggagcag
    34441 gtttgctacc aagttatgaa aatttttcac ttatacttga aaaacctcct cctacaattg
    34501 gttatttgac aattgatttt ccgaacacag gtaggagtcc gattcatgac caagctggaa
    34561 aaaatctaga taatcttgca gatgcggttt atgaagtact tgaagaatta ggaatttctg
    34621 aatatatact ttgtgcacat agttggagtg gaattttagc ttgcaaatta ctcgagaaac
    34681 caattaagcg tcagacttta gtagcaattg aaccgacaac taaaaaagtc atgtttgctg
    34741 atttttcaga aaatccttat ccaaaaatgg aagagcagat gaggctgatt gacgagtgtg
    34801 gtcctgaact ttattttaag aacttaactc aagcaacatt tagtcctgaa acgaataaaa
    34861 aaatctggga attaatgcaa gaaaaaggct tagagttgga aaatcaagat ccagaatttc
    34921 agatatctgg agagattact gaggaagatt ttgagaattt gtcgatagaa tctcatgtcc
    34981 ctgtttttat tttttgtcag acttatagag aaaaagagta cagagaatca gaatattgga
    35041 cttccaatac taaactcatt ttaggaggga atcaccatta tttacaatgg tcagaatcgg
    35101 aaaaaattgc ggctattatt cgagaattgt cagaataaga tggaaaaaag gagattacag
    35161 gagacaagat gaactacttt aatattggga aaatcgttaa tacgcaggga ttacagggtg
    35221 agatgcgagt cttgtctgtg acggattttg cagaagaacg gtttaaaaaa ggagctgagc
    35281 tggctttgtt tgatgaaaaa gatcagtttg tccaaacagt gaccatcgct agccaccgta
    35341 aacagaagaa ctttgacatt attaaattca aagatatgta ccatatcaat actatcgaaa
    35401 agtacaaggg atacagtctc aaggtcgctg aggaagattt gaatgaccta gacgatggtg
    35461 aattttacta tcacgagatt atcggtttgg aagtctatga gggtgatagc ttggttggaa
    35521 ccatcaagga aatcctgcaa ccaggtgcta atgatgtctg ggtggtcaaa cgaaaaggca
    35581 aacgtgattt gcttttacct tatatcccac cagtggttct caatgttgat attccaaata
    35641 aacgggtcga tgtggaaatc ttagaagggt tagacgatga agattgatat tttaaccctc
    35701 tttccagaga tgttttctcc actggagcac tcaatcgttg gaaaggctcg agaaaaaggg
    35761 ctcttggata tccagtatca taattttcga gaaaatgctg aaaaggcccg tcatgtagat
    35821 gatgatccct acggaggcgg tcagggcatg ttgctcagag cacaacctat tttcgattcc
    35881 tttgatgcta ttgaaaagaa aaatccgcgc gttattctcc ttgatcctgc tggaaagcag
    35941 tttgatcagg cttatgctga agatttggct caagaggaag agctaatctt tatctgtggg
    36001 cactatgagg gttatgatga gcgcattaag accttggtaa cagatgagat ttccctaggc
    36061 gactatgtcc tcactggtgg agaattggca gctatgacca tgattgatgc tacagttcgc
    36121 ctgattccag aagtgattgg caaggagtct agccaccaag atgatagttt ttcttcaggt
    36181 cttttagaat atcctcagta cacacgtccc tatgattatc gaggcatggt cgtgccagat
    36241 gtattgatga gtggccacca tgaaaagatt cgtcagtggc gattgtacga gagtttaaag
    36301 aaaacctacg agcgcagacc ggatttactt gaacattatc aactgacagt agaagaagaa
    36361 aaaatgctgg cagaaatcaa agaaaacaaa gaataaagga gaaacctatg caagtaatca
    36421 aacgtaatgg cgaaattgct gaatttaatc cagataagat ttaccaagcc atcttgaagg
    36481 cagcccaaac tgtttatgtt ttgacagatg atttgcgtca aaatcttgct caagtcacta
    36541 agaaggtagt tttggattta caagaagcca aggtggaacg tgcgactatc agtatgattc
    36601 aatctatggt tgaacatcgt ttattgggcg caggttacat taccattgca gaacactaca
    36661 tttcctatcg tttacaacgt gacttggaaa gaagtggtta tggagatcat atcgcagttc
    36721 atttacattt tgaacaaatt cgctaagaaa aaagagtggg atgaagcaac tacatctcac
    36781 tctttcttaa atctattttt tttggctgtt tggacagttg aggggacaaa tcgcaggcgt
    36841 tgaatatcgc ttatacggat aatccggctc acatttttgg caaaattttt cacgataatt
    36901 tgctggcctt gctgatcata tttgatgata tcacctgtaa agcttgtctc agacaagata
    36961 aggtgaacag cggtttgttt ctggatagcc tcttcaatag tggctgtaag ggagttggtt
    37021 tcagtctggt caggttggtc atgtccattt aaaaaatcat gtattttatc tagaacttgc
    37081 tggaatttat gtctcatatt ggcctccctt cttctatatc aatattatat aaaattttcc
    37141 taaaatctac aagattttac gaataaacta atgaaactaa aaaaggagaa gaaaatggca
    37201 gaatttacat ttgaaatcga agagcacttg ttgactcttt ctgaaaacga aaaaggttgg
    37261 accaaggaaa ttaaccgtgt gagctttaat ggtgcccctg caaagtttga tattcgtgct
    37321 tggagtccag accatactaa aatgggcaaa gggattactc tctcaaatga agaatttcaa
    37381 acgatggtgg atgcctttaa aggcaactaa tactcttcga aaatctcttc aaaccgcatc
    37441 agctttgcct tgccgtatgt atggttactg acttcgtcag tttcatccac aacctcaaaa
    37501 cagtgttttg agcagcctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat
    37561 aagttttcaa aataaaaaaa aaggatattg agtcatgata actcgatatc cttttttagt
    37621 tagtaaagta agcctgattt ttaaggcgtt gaagtagggg acggctaata aaactaatag
    37681 ccagaagttt gccaaggtct ggaatgataa agggaagaac ccccacaaca agagcttttt
    37741 caaatgccat tccagctagg aaatgcaagc tgagaatccc gccgacaaag acaagggcat
    37801 cacccaagag gtttgcaaga aaaatcttaa caacaccact cttgctgttg gttagagagg
    37861 aagtaagtcc agagtaaacg agataaaacc aaagatagcc tgcagtaggg ccaactaaag
    37921 cctgaaaacc agctccacct cctgcaaaga caggaagacc gatagcacct agaagaagat
    37981 agagtccagc agaaagtaca gcctctctcg gtctaaagac agtagcaatc aagccgattg
    38041 caaagttttg cagagtgaag ggaacaggtc caattggaag actgatttgt gccaaaacag
    38101 caatgagagc agccccaata gcagggatag cataaacgtg agcttttttc aaaatagtta
    38161 acctcttttc taatgtatag taactattat actcatatag gagtcgatgt caaccatttt
    38221 ttaaaaacaa ggtaacaaaa actgtaacag ccgattttcg tgactagtca agttggataa
    38281 tttttttgtt gattcgaaaa ttgtctgtat agataatcct attttattaa taaaaaatat
    38341 actagtaaat acttgacaaa ttaaaaaata agtgttacat taaaaatgta aacggataca
    38401 tttagatgtg ttcaatttta aaaggagggg tagttatgag atattttaac gactctagat
    38461 accataacgg tgattaaaaa cctaaatata atcaattcat attaaagtaa tatggaggga
    38521 ctaaaaagag actattattt aatcttcatc aaatatttaa attcaaggag aagtaaaatg
    38581 aaaaaaagaa ttaataagaa agcatctgtt tttctagttg caagtgtttt cagttttagc
    38641 tttgctacta cagctagtgt cgttaacgca gtaacggcac accctgctcc aagtcccatt
    38701 attgatgtat gggaatatgg tgtaaatgat aactatggtt attcgaatta ctttgttagt
    38761 tctccaagta atattggatc aaaatcttca gtaacgaatt tttggggaac agttaaagct
    38821 caggataaaa aagattatgg atgggcatat tcaagtgcaa caaaatcttg gaacgatgtt
    38881 cgcttaaatg catattggga ttattataga ttttaaaaat aatttatgaa aacaagagac
    38941 ccaattataa gattgggtct cttgtaatat ggtaattgct tatgaaatta aaattatgta
    39001 tcattggatt cttcttttgt ttgattgcta caattggtct ggtcactatt agtgataccg
    39061 aaatccccat acctttacca attgatggag ctttttctat ccaaggaaaa agtaatcttt
    39121 ctaataacga aatatacgaa atggttcgtg acttatcaaa aacggaaaag gttaccattt
    39181 acaagccaat cgttcagagt tcgggtcagt tgaagtatgt aaattttgat gatgtaaata
    39241 atgaacaatt aaagtcagca ccaatagtag ggatgtatta cacacttggg aaaatggatg
    39301 tagacagttt gaaaccactt acgatgacgg gactacaaac agtctatatg gcttatcctt
    39361 ggtatatagg aggaatatta caattcactg gaactttaag aatactgtta atgggttcaa
    39421 tttatttaac tttattagtt gttttatttg ttgttaggac gaggcagatc aaagaaggag
    39481 tgatacgacg ttctttgggc ttgcctatat acgacttaag aagagagtat ggcatttctc
    39541 ttatttttga actgattatg atggctttat tgatgatttc ttacagttct tttttgggta
    39601 atggtttctt cacctatagt tctaagttat ttttctctct gcttttaacg aattttatac
    39661 tatttcaaat cattgatctt attacttttg ttttattttg gttgacgatt cagattgaaa
    39721 agcctattga aattattaaa aacaaggcaa aaaacaagct tatttttgtc gtatggcttg
    39781 ctattatttc gattatcatc cttgtctcag gagttttttt acaagaaaca aagagtagtc
    39841 aatctagtat taacatacag atacagaatt tagtaccatg ggatacagta aaagactgga
    39901 gaaggattga gttccttgga attgaaagta actctactaa aaatagagaa gttaatgatt
    39961 cagatggtca gtatctacag atagttgctg ccttaaaaaa cttagatttc ttatatatag
    40021 aacggtcttc agcatatgtt ccagatttca tgaaaacttc acatgttata gaaaattttt
    40081 ctaaacaatt agaaaatgac gggataacga atccagaaat aaataaagag ttaatttata
    40141 tcaaccaaac aggtgctaac ctacaaaata aagtgaatgg aacaaactat catcttttag
    40201 ataataagat agcaaccatc tatattcctg agaagtggaa agaaaaccag aaatctattg
    40261 agaatacagt tgtagcagaa caatttattg gaacaaacta tacaagagaa cagcttgcag
    40321 ttcaaataat tcctgatggg gaaaaaattt tttattttaa tgaggatgct gataataatc
    40381 ttaaaatgaa agatatttta ccgctggcaa atgtagcaga tagcaaagat aatatagttg
    40441 ttgtgttaga tacggacaaa atgatggaaa ataataagtt ttctttggct agtaacatac
    40501 tatataaatc tctttttagt cctgaagcgg taaaaaaaat aaatgagatg actgtcctgt
    40561 tgaatttctc aatgaatcca gtagatgttt accaaattgt gaaattaaag attcaatcct
    40621 tagagcacca aattttactt tcacaaatct tgcagaagat aatttacagt attgtcttta
    40681 tactcattta tcaatatgtc c
//
LOCUS       DAVLVF010000009        40237 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVLVF010000009 DAVLVF010000000
VERSION     DAVLVF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40237
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.009"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            5..75
                     /locus_tag="VYN66_000437"
     tRNA            5..75
                     /locus_tag="VYN66_000437"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="VYN66_000438"
     tRNA            110..183
                     /locus_tag="VYN66_000438"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            266..355
                     /locus_tag="VYN66_000439"
     tRNA            266..355
                     /locus_tag="VYN66_000439"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Ser,seq:tga)
     gene            366..439
                     /locus_tag="VYN66_000440"
     tRNA            366..439
                     /locus_tag="VYN66_000440"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Met,seq:cat)
     gene            443..515
                     /locus_tag="VYN66_000441"
     tRNA            443..515
                     /locus_tag="VYN66_000441"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Phe,seq:gaa)
     gene            528..608
                     /locus_tag="VYN66_000442"
     tRNA            528..608
                     /locus_tag="VYN66_000442"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:562..564,aa:Tyr,seq:gta)
     gene            613..683
                     /locus_tag="VYN66_000443"
     tRNA            613..683
                     /locus_tag="VYN66_000443"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:645..647,aa:Trp,seq:cca)
     gene            698..770
                     /locus_tag="VYN66_000444"
     tRNA            698..770
                     /locus_tag="VYN66_000444"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:731..733,aa:His,seq:gtg)
     gene            784..855
                     /locus_tag="VYN66_000445"
     tRNA            784..855
                     /locus_tag="VYN66_000445"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:816..818,aa:Gln,seq:ttg)
     gene            865..948
                     /locus_tag="VYN66_000446"
     tRNA            865..948
                     /locus_tag="VYN66_000446"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:899..901,aa:Leu,seq:caa)
     gene            1055..2332
                     /locus_tag="VYN66_000447"
     CDS             1055..2332
                     /locus_tag="VYN66_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV1494513.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2408..3892
                     /locus_tag="VYN66_000448"
     CDS             2408..3892
                     /locus_tag="VYN66_000448"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV1494514.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3953..5233
                     /locus_tag="VYN66_000449"
     CDS             3953..5233
                     /locus_tag="VYN66_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1494515.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5237..5857
                     /locus_tag="VYN66_000450"
     CDS             5237..5857
                     /locus_tag="VYN66_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1494516.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6023..7135
                     /locus_tag="VYN66_000451"
     CDS             6023..7135
                     /locus_tag="VYN66_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1494517.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7458..7883
                     /locus_tag="VYN66_000452"
     CDS             7458..7883
                     /locus_tag="VYN66_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1494518.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7894..8253
                     /locus_tag="VYN66_000453"
     CDS             7894..8253
                     /locus_tag="VYN66_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV1494519.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            8360..9004
                     /gene="pcp"
                     /locus_tag="VYN66_000454"
     CDS             8360..9004
                     /gene="pcp"
                     /locus_tag="VYN66_000454"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1494520.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(9032..9889)
                     /locus_tag="VYN66_000455"
     CDS             complement(9032..9889)
                     /locus_tag="VYN66_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV1494521.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10019..11161
                     /gene="tgt"
                     /locus_tag="VYN66_000456"
     CDS             10019..11161
                     /gene="tgt"
                     /locus_tag="VYN66_000456"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV1494522.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11259..13076
                     /locus_tag="VYN66_000457"
     CDS             11259..13076
                     /locus_tag="VYN66_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1494523.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILVAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13229..14380
                     /gene="nagA"
                     /locus_tag="VYN66_000458"
     CDS             13229..14380
                     /gene="nagA"
                     /locus_tag="VYN66_000458"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV1494524.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14543..15601
                     /locus_tag="VYN66_000459"
     CDS             14543..15601
                     /locus_tag="VYN66_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV1494525.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15752..16117
                     /locus_tag="VYN66_000460"
     CDS             15752..16117
                     /locus_tag="VYN66_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV1494526.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16193..17134
                     /locus_tag="VYN66_000461"
     CDS             16193..17134
                     /locus_tag="VYN66_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV1494527.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17082..18098
                     /locus_tag="VYN66_000462"
     CDS             17082..18098
                     /locus_tag="VYN66_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV1494528.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVIQMRAGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18100..18426
                     /locus_tag="VYN66_000463"
     CDS             18100..18426
                     /locus_tag="VYN66_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV1494529.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            18419..18823
                     /locus_tag="VYN66_000464"
     CDS             18419..18823
                     /locus_tag="VYN66_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV1494530.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSSSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18828..19088
                     /locus_tag="VYN66_000465"
     CDS             18828..19088
                     /locus_tag="VYN66_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494531.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19051..19512
                     /locus_tag="VYN66_000466"
     CDS             19051..19512
                     /locus_tag="VYN66_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1494532.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19490..19936
                     /locus_tag="VYN66_000467"
     CDS             19490..19936
                     /locus_tag="VYN66_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1494533.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKNQKRSLKRKRIPSQFNYNALNPE"
     gene            20039..20626
                     /locus_tag="VYN66_000468"
     CDS             20039..20626
                     /locus_tag="VYN66_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1494534.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKVILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMLEQARLKVEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVHSQILYSAEDLFQGNHSEFFLIVAQKSLA"
     gene            20687..21640
                     /locus_tag="VYN66_000469"
     CDS             20687..21640
                     /locus_tag="VYN66_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1494535.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEMDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSNLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21691..22881
                     /locus_tag="VYN66_000470"
     CDS             21691..22881
                     /locus_tag="VYN66_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV1494536.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYFAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23030..23401
                     /gene="rnpA"
                     /locus_tag="VYN66_000471"
     CDS             23030..23401
                     /gene="rnpA"
                     /locus_tag="VYN66_000471"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV1494537.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23376..24200
                     /locus_tag="VYN66_000472"
     CDS             23376..24200
                     /locus_tag="VYN66_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1494538.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24219..25205
                     /locus_tag="VYN66_000473"
     CDS             24219..25205
                     /locus_tag="VYN66_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV1494539.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25256..25879
                     /locus_tag="VYN66_000474"
     CDS             25256..25879
                     /locus_tag="VYN66_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV1494540.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26010..27467
                     /locus_tag="VYN66_000475"
     CDS             26010..27467
                     /locus_tag="VYN66_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1494541.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27490..27771
                     /locus_tag="VYN66_000476"
     CDS             27490..27771
                     /locus_tag="VYN66_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1494542.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27848..28333
                     /locus_tag="VYN66_000477"
     CDS             27848..28333
                     /locus_tag="VYN66_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1494543.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGLYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28350..29015
                     /locus_tag="VYN66_000478"
     CDS             28350..29015
                     /locus_tag="VYN66_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV1494544.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            29019..29882
                     /locus_tag="VYN66_000479"
     CDS             29019..29882
                     /locus_tag="VYN66_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV1494545.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29884..30588
                     /locus_tag="VYN66_000480"
     CDS             29884..30588
                     /locus_tag="VYN66_000480"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV1494546.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30767..32440
                     /locus_tag="VYN66_000481"
     CDS             30767..32440
                     /locus_tag="VYN66_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1494547.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINKEIVVQAFLEDI"
     gene            32552..33643
                     /gene="ulaG"
                     /locus_tag="VYN66_000482"
     CDS             32552..33643
                     /gene="ulaG"
                     /locus_tag="VYN66_000482"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV1494548.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33757..35733
                     /gene="tkt"
                     /locus_tag="VYN66_000483"
     CDS             33757..35733
                     /gene="tkt"
                     /locus_tag="VYN66_000483"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1494549.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            35850..36149
                     /gene="yajC"
                     /locus_tag="VYN66_000484"
     CDS             35850..36149
                     /gene="yajC"
                     /locus_tag="VYN66_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1494550.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36196..36624
                     /locus_tag="VYN66_000485"
     CDS             36196..36624
                     /locus_tag="VYN66_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV1494551.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLCQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36626..37036
                     /locus_tag="VYN66_000486"
     CDS             36626..37036
                     /locus_tag="VYN66_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494552.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37336..39987
                     /gene="adhE"
                     /locus_tag="VYN66_000487"
                     /gene_synonym="adhC"
     CDS             37336..39987
                     /gene="adhE"
                     /locus_tag="VYN66_000487"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV1494553.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12207 a         7212 c         9065 g        11753 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttaacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaact attttggagg attacccaag tccggctgaa gggaacggtc
      301 ttgaaaaccg tcaggcgtgt aaaagcgtgc gtgggttcga atcccacatc ctccttttat
      361 attaacgcgg gatggagcag ctcggtagct cgtcgggctc ataacccgaa ggtcgtaggt
      421 tcaaatcctg ctcccgcaat aaggctcggt agctcagttg gtagagcaat ggattgaagc
      481 tccatgtgtc ggcggttcga ttccgtctcg cgccatttat atattttgga agggtagcga
      541 agaggctaaa cgcggcggac tgtaaatccg ctccttcggg ttcgggggtt cgaatccctc
      601 cccttccatt ttacgggcat agtttaaagg tagaactaag gtctccaaaa ccttcagtgt
      661 gggttcaatt cctactgccc gtgttaatag aattatggcg ggtgtggtga agtggttaac
      721 acaccagatt gtggctctgg catgcgtggg ttcgatcccc atcactcgcc tattttatat
      781 tattggggta tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga
      841 atccagctac cccagttact atttgccggc gtggcggaat tggcagacgc gctggactca
      901 aaatccagtg tccgcaagga cgtgccggtt cgaccccggc cgccggtata gtatagtgtt
      961 aggaacgttg ttattcttcg ttcctttttt atattatttt tggtataatt atagttattc
     1021 aaattttatt tagattaaga aagtgtaggg gagtatgtct tgttctatcg atttattaaa
     1081 acatcggtat ttgaaaaata ttaaagaaaa tcctgaattg tttgtcggaa ttgagttgga
     1141 gtatcctgtt gcaagtttag aaggggatgc tacagatgtt gaagttatga aggatctatt
     1201 tcattattta gtttctactt tggatctcac cgtagcaaag gtagatgatt ttggcaatct
     1261 gatccagtta gtagatccga taagtcagga tgctatttta tttgaagttt cctatacaac
     1321 gattgagttt gcatttggta aggctgaaac gattcaagag gtcgaaaatc gtttcaataa
     1381 ttatatgaat gtaattcaga gaaagttagc tgaatcaaat catgctattg ttggctgtgg
     1441 tatccatccc aactgggata aaaatgagaa ttgtccagtg gcttatccac gctatcagat
     1501 gttgatggat tatttgaatt tgagtagaaa tattattaaa tcagatttac atcatttccc
     1561 tgaatatggt acttttatct gtgggagcca ggttcagctg gatatttcaa aaaccaacta
     1621 cttacgggtg attaatgctt ttactcaaat tgaagcggct aaggcttatt tatttgcaaa
     1681 ctctgaattt tcgggtgcgg attgggatac gaaaatttca agggatattt tctgggaaga
     1741 atctatgcat ggtatctatc cagagaatgt tggggtcaat gctagactcc ttaatgatga
     1801 agctgatttt tttgactatc taaatcattc tgcgattttt actgcggaac gtgatgggca
     1861 gacctattat ttttatccta ttcaggctgg ggactatttg gctacgtccg aaatccaagc
     1921 atttgctctg aatggggatg aggttattat ttacccccaa gagaaggatt ttgaaactca
     1981 tcgtagttac cagtaccaag atttaacgac tcgaggaaca gttgagtttc gtagtgtgtg
     2041 tacacagcca cttgatagga cttttgcttc tgcagctttt cacttgggat tattggttaa
     2101 tttagacaag ttagaagctt acttagaaac agcacctttc tttaaagtat ttggttatga
     2161 ttacaagtct ttaaggagac aattttctaa gaaaaatctt acagatgagg aagaaactac
     2221 gattattgaa ttttccaaag acttactcct actagctgag gagggactag tggtgagaaa
     2281 taaggaagaa atgacctatt tacagccttt gagagaagaa ttgagcctat aatttctctt
     2341 ataaagggag aattttctga aaaatcatga tataatggac gagactatag ataaaggata
     2401 gagagtaatg acattagttt atcaatcaac gcgtgatgcc aacaatacag taactgccag
     2461 ccaagcaatt ttgcaaggtt tggcgacgga cggcggtttg tttacaccgg atacttatcc
     2521 aaaggtagat ttgaactttg acaaattgaa agatgcttct taccaggaag ttgctaagct
     2581 agttttgtca gcatttttag atgactttac agttgaggag ttggactact gtatcaacaa
     2641 tgcctacgat agcaaatttg atactccagc tattgcacca ttagtgaaat tagatgggca
     2701 atacaatttg gaacttttcc atggttcaac gattgccttt aaggatatgg ccttgtctat
     2761 tttgccatac tttatgacga ctgctgctaa gaaacatggt ttggagaaca agattgttat
     2821 cttgacagcg acatctggtg acacggggaa agctgctatg gcggggtttg cgaatgtgcc
     2881 tggtactgag attatcgtct tttatccaaa ggatggtgtc agcaagattc aagagttaca
     2941 aatgaccact cagactggcg acaatactca tgttattgct attgatggta actttgacga
     3001 tgcgcaaaca aatgtgaagc acatgtttaa cgacgtggct cttcgtgaaa aattgactac
     3061 caacaagttg caattttcat cagctaactc tatgaacatt ggtcgtctgg tgccacaaat
     3121 tgtttattat gtttatgctt acgctcaatt ggttaagact ggtgaaattg tagctggtga
     3181 aaaggttaac ttcacagtac caacaggaaa ctttggaaat atcttggctg ccttttatgc
     3241 caaacaaatt ggtttgccag ttggtaaatt aatctgtgct tcaaatgaca acaatgtttt
     3301 gacagacttc tttaaaacac gtgtctatga caaaaaacgt gagtttaagg taacaaccag
     3361 cccatctatg gatatcttgg tatcttcaaa cttggagcgc ttgattttcc atcttttggg
     3421 aaataatgct gaaaagacaa ctgaacttat gaatgccttg aacacgcaag gacaatataa
     3481 gttgacagac tttgatgcag agattttgga cctctttgca gctgaatatg cgactgagga
     3541 agaaacggca gcagagatca agcgtgtttg tgagttagat tcttatatcg aggaccctca
     3601 tacagctgtt gcttcagcag tttataaaaa ataccaatcg gccactggag atgtaactaa
     3661 gacagtgatt gcttcaacag ctagtccata caagttccca gtagttgcag tagaagctgt
     3721 aactggaaaa gcaggtttaa cagactttga agccttggct caattacatg aaatctcagg
     3781 cgttgcagtg ccaccagcag ttgatgggct tgaaatagct ccaattcgtc acaagacaac
     3841 agtggcagct gctgacatgc aagcagcggt tgaggcttat ttaggacttt aagacagagg
     3901 gagcaaactc ggttgggaaa ccaactgagt ttcttttcat caggaggaga gattgtttaa
     3961 gaaaaataaa gacattctta atattgcatt gccagctatg ggtgaaaact ttttgcagat
     4021 gctaatggga atggtggaca gttatttggt tgctcattta ggattgatag ctatttcagg
     4081 ggtttcagta gctggtaata ttatcaccat ttatcaggcg attttcatcg ctctgggagc
     4141 tgctatttcc agtgttattt caaaaagcat agggcagaaa gaccagtcga agttggccta
     4201 tcatgtgact gaggcgttga agattacctt actattaagt ttccttttag gatttttgtc
     4261 catcttcgct gggaaagaga tgataggact tttggggacg gagagggatg tagctgagag
     4321 tggtggactg tatctatctt tggtaggcgg atcgattgtt ctcttaggtt taatgactag
     4381 tctaggagcc ttgattcgtg caacgcataa tccacgtctg cctctctatg ttagtttttt
     4441 atccaatgcc ttgaatattc ttttttcaag tctagctatt tttgttctgg atatggggat
     4501 agctggtgtt gcttggggga caattgtgtc tcgtttggtt ggtcttgtga ttttgtggtc
     4561 acaattaaaa ctgccttatg ggaagccaac ttttggttta gataaggaac tgttgacctt
     4621 ggctttacca gcagctggag agcgacttat gatgagggct ggagatgtag tgatcattgc
     4681 cttggtcgtt tcttttggga cggaggcagt tgctgggaat gcaatcggag aagtcttgac
     4741 ccagtttaac tatatgcctg cctttggcgt cgctacggca acggtcatgc tgttggcccg
     4801 agcagttgga gaggatgatt ggaaaagagt tgctagtttg agtaaacaaa ccttttggct
     4861 ttctctgttc ctcatgttgc ccctgtcctt tagtatatat gtcttgggtg taccattaac
     4921 tcatctctat acgactgatt ctctagcggt ggaggctagt gttctagtga cactgttttc
     4981 actacttggg acccctatga cgacaggaac agtcatctat acggcagtct ggcagggatt
     5041 aggaaatgca cgcctccctt tttatgcgac aagtatagga atgtggtgta tccgcattgg
     5101 gacaggatat ctgatgggga ttgtgcttgg ttggggcttg cctggtattt gggcagggtc
     5161 tctcttggat aatggttttc gctggttatt tctacgctat cgttaccagc gctatatgag
     5221 cttgaaagga taggaaatgc aaaaaacagc ttttatttgg gatttagacg ggactttatt
     5281 ggactcttac gaagcgattt tatcagggat tgaggagact tttgctcagt tttctattcc
     5341 ttatgataag gagaaggtga gagagtttat cttcaagtat tcggtgcaag atttgcttgt
     5401 gcgggtggca gaagatagaa atctggatgt tgaggtgcta aatcaggtgc gtgcccagag
     5461 tctggctgag aagaatgctc aggtagtttt gatgccaggt gcgcgtgagg tgctagcttg
     5521 ggcagacgaa tcaggaattc agcagtttat atatactcat aaggggaaca acgcttttac
     5581 cattctcaag gacttggggg tggaatccta ttttacagag attttaacca gtcagagtgg
     5641 ctttgtgcgg aagccaagtc cagaagcggc tacctatctg ctagataagt atcagttgaa
     5701 ttctgataat acttattata taggggatcg gactctggat gtggaatttg cccagaatag
     5761 tgggattcaa agcatcaact ttttagagtc tacttatgaa gggaatcaca ggattcaagc
     5821 gttagcagat atttcccgta tttttgagac taagtgataa aaagattgtg tcagttttgt
     5881 gacagagacc taacaaacta tttcaagtaa cctagtttgt tacaaggaat agacagttct
     5941 gttaaatagg cccgagaggg ctttttttct acattttttg tgttatgata gacaggtact
     6001 catttgaaag gaatttgaaa gaatgaagaa aagaatgtta ttagcgtcaa cagtagcctt
     6061 gtcatttgcc ccagtattgg caactcaagc agaagaagtt ctttggactg cacgtagtgt
     6121 tgagcaaatc caaaacgatt tgactaaaac ggacaacaaa acaagttata ccgtacagta
     6181 tggtgatact ttgagcacca ttgcagaagc cttgggtgta gatgtaacag tgcttgcgaa
     6241 tctgaacaaa atcactaata tggacttgat tttcccagaa actgttttga caacgactgt
     6301 caatgaagca gaagaagtaa cagaagttga aatccaaaca cctcaagcag actctagtga
     6361 agaagtgaca actgcgacag cagatttgac cactaatcaa gtgaccgttg atgatcaaac
     6421 tgttcaggtt gcagaccttt ctcaaccaat tgcagaagtt acaaagacag tgattgcttc
     6481 tgaagaagtg gcaccatcta cgggcacttc tgtcccagag gagcaaacga ccgaaacaac
     6541 tcgcccagtt gaagaagcaa ctcctcagga aacgactcca gctgagaagc aggaaacaca
     6601 agcaagccct caagctgcat cagcagtgga agtaactaca acaagttcag aagcaaaaga
     6661 agtagcatca tcaaatggag ctacagcagc agtttctact tatcaaccag aagagacgaa
     6721 aataatttca acaacttacg aggctccagc tgcgcccgat tatgctggac ttgcagtagc
     6781 aaaatctgaa aatgcaggtc ttcaaccaca aacagctgcc tttaaagaag aaattgctaa
     6841 cttgtttggc attacatcct ttagtggtta tcgtccagga gacagtggag atcacggaaa
     6901 aggtttggct atcgacttta tggtaccaga acgttcagaa ttaggggata agattgcgga
     6961 atatgctatt caaaatatgg ccagccgtgg cattagttac atcatctgga aacaacgttt
     7021 ctatgctcca ttcgatagca aatatgggcc agctaacact tggaacccaa tgccagaccg
     7081 tggtagtgtg acagaaaatc actatgatca cgttcacgtt tcaatgaatg gataaacccg
     7141 acttgataac atcattttga cgaatgagat ctagctttcg tgatggaaag cgattctcgt
     7201 tcgttttttc tttgtcatac tcttcgaaaa tctcttcaaa ccacgtcagt tttatctgaa
     7261 acttcaaagc tgtgctttga gcaacctgcg actagcttcc tagtttgctt tttgattttc
     7321 attgagtatc aatttgaatg gaaaatggaa agttatcatc ttgtaatgag ttaagcaaca
     7381 ttcttgcaat ctattttact ttatatcaca attaattagt caaatattga taaatcaata
     7441 aaaagagagg ggaagaaatg ctagagattc aagatttact gtatcaactc cgcttgtctg
     7501 agcaagcgag tacgcaattg tttgaaaaaa ggcttgggat tagtttgaca cggtatcaga
     7561 ttttactgtt tttgctggag cattctcctt gtaaccaaat ggcggttcag gagcgtttga
     7621 aaattgatca ggctgctttg acacggcatt tcaaaatttt ggaaacggaa ggtttggtgg
     7681 agcgtcatcg taatcctgaa aatcagcggg aagtgttggt agaggctgcg aagtatgcca
     7741 aggagcagtt agtggtgaat ccccctctgc aacatatcag ggttaaggaa gagatagaaa
     7801 gtatcttaac agagtttgag agaacagaac tcagccgttt attaaataaa ttggttttgg
     7861 gtattgaaaa tatagaaatt taaggagaaa tagatgtcaa ttattttaac aacgatcgtt
     7921 gctttggagc atttttacat tttttatttg gaaagtattg ccacgcaatc agatgcgact
     7981 agtcgtgtat ttaatatgga aaaggaagaa ttggctcatc cgtcagtaag ttcattgttc
     8041 aaaaatcaag gaatttataa ggctctgcta ggagtctttc tcttgtatgt catttatttc
     8101 tcacagaatt tagaaattgt gactattttt gtcttatttg tgattggtgc tgcgacttac
     8161 ggctctttaa cagcggataa aaaaattatt ttgaaacaag gtggatcagc tattttggcc
     8221 ttgattagta ttttactctt taaatacact tgaaggtcga ttctaatctc gctaatcctt
     8281 tttaatccag aataagggaa atatgttata cttgttttta agaaaaaagt ctcattgaat
     8341 tggttttgag gagttagaaa tgaaagtatt agtgacaggt tttgagccct ttggagggga
     8401 aaagggcaat ccagctttgg aggccattaa aggtttacca gctgaaatcc atggtgctga
     8461 ggtccgttgg ctagaggtgc cgacagtttt tcacaaatct gctcaagtat tggaagaaga
     8521 gatgaatcgt tatcaacctg actttgtcct ttgtattggg caagctggtg gaagaactag
     8581 tttgacacct gaacgagtga ccattaatca agacgatgca tgcatttctg ataacgaaga
     8641 taatcaaccg attgaccgtc ccattcgccc agatggtgct tcggcctact ttagtagttt
     8701 gccgattaaa gcgatggttc aagctataaa aaaagagggc ttaccggcct ctgtttccaa
     8761 tacggcaggg acttttgtct gcagccattt gatgtatcag gctctctatt tggtagaaaa
     8821 gaaatctcca tatgttaagg caggttttat gcatattcct tatatgatgg aacaggtggt
     8881 gaacagaccg actactccag ctatgagttt agtggatatt cggcgaggga tagaagcagc
     8941 aatcggcgct ataatagaac atggagatca ggaactcaag ttggtaggcg gagaaactca
     9001 ttgatagaaa aaagcttgag gagaaaaacc ttcaagcttt tggacgtttt cgggccaata
     9061 ctgctcggta aaacataatt ttagtgcatt ggatataagg taggagtgaa aaactagcaa
     9121 tgccaaaggt aatccaattg aggaagtacc aaggaagaag ctgtaaatct aggacaaagt
     9181 gctggaactt gtagcccttc ataaaggaac ggctagtttt taggattcgt cttggtggga
     9241 cctgtcctag gtctagacta taacagagaa gaaattccac ctgtgaatag gcataatact
     9301 gtggaatata gaggatattt cctacaatga tcaagatgag acttgcaaga aagtagagtc
     9361 caaagaccat gaggaaacgc tcggtttcaa ctgatgagag atctagattt ggaaactcag
     9421 gatgtagggt gacgaatttt ttggctaaaa agctactata aaagaggagg taaatcccaa
     9481 gtaaattagg gatactccat aaaaagagat agaaacgttt gagaagtagg gtcaaaaagg
     9541 tttgagaaaa gcgctcctca tcaaagagag ctaggctgtt ttttacagat ggctccgttt
     9601 tagaatcttt catgagtgtc agtgttgcat agacggaact ggtcaaaaga atagtcccga
     9661 taaaggagac tagtagagga aagaggtagg tttgaagtat ttggccaagt atgctgaaaa
     9721 atggctgttc taaaacagtc ccgtggatcc gagataaggg attaagaaaa ccagataaga
     9781 tgaccagcat actgggaagg atatagagga gaaagagacg gggggtgtca gcctgaaaat
     9841 gttttgactc ctgacgaatt gtttttaaat caatttttgg atagttcatt ctcttattat
     9901 accatagttc ttatacatag ttcgtgacag ttcctacttt ttttgataaa atcatacagt
     9961 gtgtccttgg gcacactgta tgaactggga ctgtctttcc cagcttcgga ggtaaaaaat
    10021 gtcagattca ccaatcaaat atcgtttgat taagaaagaa aaacacacag gagctcgtct
    10081 gggagaaatc atcactcccc acggtacctt tccgacacct atgtttatgc cagttgggac
    10141 acaagccact gtcaaaactc agtcacctga agaattgaag gagatgggtt cgggaattat
    10201 cctatcaaac acctatcatc tctggcttcg ccctggagat gaactcattg cacgcgctgg
    10261 tggtctccac aagttcatga attgggacca gcctatcttg acagatagtg gtggttttca
    10321 ggtttattct ttagcagata gccgtaatat cacagaagaa ggagtaacct ttaaaaatca
    10381 tctaaatggt tctaagatgt tcctatcccc agaaaaagcc atctctattc agaataatct
    10441 gggttcagac atcatgatgt cctttgatga atgtcctcag ttttatcaac cttatgacta
    10501 cgttaagaaa tcgatcgagc gtaccagccg ttgggctgag cgtggtttga aggctcaccg
    10561 tcgtccacat gaccaaggtt tgtttggaat tgtgcaaggt gcaggatttg aagaccttcg
    10621 ccgccaatca gctcatgatc ttgtcagcat ggatttctca ggctactcta tcggtggttt
    10681 ggcagtggga gaaacccatg aagagatgaa tgcggtcttg gactttacaa ctcaactgct
    10741 acctgaaaat aaacctcgtt atctgatggg tgtgggagcg ccagatagct tgatcgatgg
    10801 ggtcattcgt ggggtggata tgtttgactg tgtcttaccg actcgaattg ctcgtaacgg
    10861 gacttgtatg accagtcaag gacgtttggt tgtgaaaaat gcccagtttg ctgaggactt
    10921 tacgccactg gatcctgagt gtgactgcta cacatgtaat aactatacac gcgcttacct
    10981 tcgtcacctg ctcaaggctg atgaaacctt tggtatccgc ttgactagct accacaatct
    11041 ttacttcttg cttaacctga tgaagcaagt gcgacaagcc atcatggatg acaatctctt
    11101 ggaattccgt gagtattttg tggaaaaata tggctataat aagtcaggac gtaatttcta
    11161 aaatggaatt gatataaaaa aatcctaagt tttctcttag gatttttctt ctttttttga
    11221 tagaataaag tgtacaatga aaggaagaat aaactcgtat gcgcattaaa tggttttcct
    11281 tgattaggat tataggttta cttttggtac tcttgtatca cttctttcag acgatctttc
    11341 ctggaggatt tttcggggta gatgtctttt tcacattttc aggtttcctg attacagctc
    11401 tactcattga agaattttct aagaacaatg agattgattt gataggattt tttagaagac
    11461 gcttttatcg gattgtgcca cctgtggttt tgatggtctt ggtgaccatg ccttttactt
    11521 tcttggttcg ccaagactat gttgctggaa ttggtggcca gattgcgggc gtcttaggct
    11581 ttatgaccaa cttctatgaa ctcctaacag gtgggagtta tgaatctcag ttcattcctc
    11641 atttgtttgt tcataattgg agtttggcag ttgaggttca ctactatatt ctttggggat
    11701 tggcagtttg gttcttatcc aaacaagcta aatcaaatgg tcagttgaag gggatggtct
    11761 ttctcttatc tgctgctgcc ttcttgatca gtttcttctc catgtttatt ggtagttttc
    11821 tagtgacctc ttattcctct gtttatttct ccagtttaac tcatgtctat ccattctttt
    11881 tgggaagtat gttagcaact attgtaggtg ttcgtcagac gacttccctc gtcaagcagt
    11941 tggataaaat ctgggattta cgaaagactt tggtagtttt tggaggaggc tttggtttcc
    12001 tagttctttt gactttcttt gtcaaattca cttatctttt tgcctatctt atcggcttct
    12061 tgcttgccag tcttgcagct cttgccatga ttctggcggc gcgtgtctta catgaaaaga
    12121 cacatcatat acaggagccg aagattatca gctttttagc ggatactagc tatgcggttt
    12181 atcttttcca ttggcctttc tatatcattt tctcacagtt gacatcaaat cttcttgctg
    12241 tattactgac tctgatttgt tcttatggct ttgccagtct gtcattctat gtattggaac
    12301 cttggattgc aggcaagaac acacctattt tacaaactct tcgtcccctg ccttatattc
    12361 acgcaattct tgtagcaggt acaggaatct tgaccatcat tgtctgcacg gtgaccttgt
    12421 tggcaccaca agtgggagcg tttgagacag acttgactgt caatggcttg aagcaagctg
    12481 caacaaatat tggccagacc aaggtgatgg cagaacgggc agatgcaaac agtttgggaa
    12541 ttgctgatgg cactatgtta attggtgact cagtggcttt aagggcaaat acagcactac
    12601 agacagctct tcctggagca cagattaacg cgcaggtcag cgtaacaacc aagaccgcaa
    12661 atgaaatcat gctaaataat agccagaata aatttttacc taagacggtg gtcattgcga
    12721 ctggggtaaa taatcctgag aattacaagg atgactggga cagtatcgtg aaaaatcttc
    12781 ctaagggaca ccatatgatt ttggtgactc cttatgaggg agataagaca aaagagacct
    12841 atgccatcgt tgagaaggct gctgcctata tgagagaatt ggcagagaag acaccttaca
    12901 ttacgatagc agattggaat caagttgcga aagagcatcc agaaatttgg gctggaacag
    12961 accaggttca tttcgggagt gagagtagca ctatcgaagc aggagcaaaa ttgtatgcag
    13021 atacgattgc cacagctttg cagacagctc aagacaagcc ggttaaatca aaataacttg
    13081 tattaaaaaa gagaagagtt ggagaaatcc agctctttta aaatatctcc aaaaaatagg
    13141 tctataccat ttacaaataa aaaagaaagg tttataatgt aattgacata ataaattgta
    13201 gaatcaatct tttaaggagg ttaacattat gcctaactat attaaagcgg atcagttttt
    13261 ctacccacac ggagttcgtc gaggtggtta cttggaactt gtggacggca agtttgggaa
    13321 acatgtagag cagattcctg aaggggctga ggtgattgac tatacaggtt atagcattgc
    13381 cccaggtctt gtggatactc atattcatgg atatgcaggt gtagatgtga tggacaacaa
    13441 cattgaaggt acattgcata ctatgagtga aggacttctt agtaccggtg ttaccagttt
    13501 cttacccaca actttaacag ccacttatga gcaattgctt gcagtcactg aaaatcttgg
    13561 aaaccattat aaagaagcaa caggtgctaa gattcgtggg atttattatg aaggtccata
    13621 tttcacagaa acttttaagg gggcacaaaa tccaacttat atgagagacc cgggtgttga
    13681 ggagtttcat tcttggcaaa aagcggcaaa tggcttgctt aataaaattg cccttgcacc
    13741 agaacgtgat ggggtggaag actttgttcg tacagttacg ggcgaaggtg tgacggttgc
    13801 tcttggacat tcaaacgcga cttttgatga agccaaaaaa gcagtcgatg ctggagcgag
    13861 tgtttgggtg catgcctata atggaatgcg tgggttgact caccgtgaat tgggtatggt
    13921 tggagccatg taccaattgc cacataccta tgcagagttg atctgtgatg gtcaccacgt
    13981 agatccaaag gcttgcgaaa ttcttatcaa acaaaaagga acagaaaaca tcgctcttat
    14041 cacagactgt atgacagctg ggggattgga agacggagat tatatgttgg gagaattccc
    14101 agttgtcgtt gcaaatggaa ctgcacgcct caaatcgaca ggtaacttgg caggttctat
    14161 cctcaaactc aaagatggtt tgaagaatgt ggtcgaatgg ggaattgcga atccgcatga
    14221 agcagtcatg atggccagct tcaacccagc taaatccgtt cacatcgatg acgtctgtgg
    14281 ccaaatccgt gaaggctacg acgctgactt catcgtatta gataaagatt tggaattggt
    14341 agcaacctat ctagatggcg taaaacgtta tcaagcataa gagagaaggc agaagttaga
    14401 gactagcttc tgctttttta tacagggtac tttactggta aataaaaagt taaaaaaatc
    14461 acaaaaaaag cttgaagaaa caaagaaaaa ggcttacaat tgaactataa atattacaaa
    14521 taaaaaaacg gaggagtgct ttatgaaagc ctatacttat gttaaaccag gacttgcttc
    14581 ttttgttgat gtagacaaac cagttattcg caagccaaca gacgctattg tgcgtattgt
    14641 aaaaaccact atttgtggaa cagacctcca tattatcaaa ggggatgttc ctacttgcca
    14701 aagtggtacc attcttggcc acgaagggat tgggattgtt gaagaagttg gggaaggagt
    14761 ttccaacttc aaaaaaggcg acaaggtctt gatttcttgc gtctgtgcct gtggtaaatg
    14821 ctactactgt aaaaaaggaa tttatgccca ctgtgaagac gaagggggct ggattttcgg
    14881 tcacttgatt gatggtatgc aggctgaata tctacgtgtc cctcatgcag ataatactct
    14941 ttaccatact ccagaagact tgtcagatga agctttggtt atgctgtcag acattctgcc
    15001 tactggatat gaaattggtg tcttaaaagg gaaagtagaa cctggttgca gcgtagccat
    15061 tattggttca ggtccagttg gattggctgc tcttttaaca gcccaattct attcaccagc
    15121 taaattgatt atggtagacc tagacgataa ccgcttggaa actgccctat cattcggtgc
    15181 gactcataag gttaattctt cagaccctga aaaagccatt aaagaaattt atgatttgac
    15241 agatggtcgt ggtgtggatg tcgctatcga agctgttggt attcctgcaa catttgattt
    15301 ctgtcaaaag attatcggtg tagacggaac ggttgccaac tgtggtgtgc atggtaaacc
    15361 agttgaattc gatttagata aactttggat tcgcaacatc aatgtaacaa ctggtttggt
    15421 atctacaaat acgactccac aattgttgaa agcacttgaa agtcataaga ttgaaccaga
    15481 aaaattggta actcactatt tcaaactcag tgaaattgaa aaagcctatg aagtcttcag
    15541 taaggcagca gaccaccatg ccattaaggt cattatcgaa aacgatatct cagaagccta
    15601 agtagtaaaa atatttttgt acataagtaa atagaaattc agtcatccat cagatggctg
    15661 gattttttat caaaaaatta agaaatgagc atatttcttt ccttgtctgg cggaattggt
    15721 tataatatac ggtacaaagg aatgaatgaa tatgtatcgt gttatagaaa tgtacggaga
    15781 ttttgaaccg tggtggttct tagaaggttg ggaagaagat attgtagcaa gtagaaaatt
    15841 tgaccagtat tatgatgctc tcaaatacta caaaacttgc tggtttagat tggaacaaga
    15901 atcccctctt tataaaagta gaagcgactt gatgaccatt ttttgggacc cggaagacca
    15961 acgctggtgt gatgaatgtg atgagtattt acaacaatac cattctttgg ctcttttgca
    16021 ggatgagcag gttatcccag acgaaaaact acgctcaggc tatgaaaaac aaaccagtca
    16081 ggaaaggaat cgttcttgcc gtatgaaatt aaaatagaga aaagtaactt ttttggagtt
    16141 gcttttttta tttttctaac gctttgcgaa tagtataggt gaggaggtaa gtatggttca
    16201 agaaattgca caagaaatca ttcgttcagc tcggaaaaaa gggacgcagg atatttattt
    16261 tgtccctaag ttagacgcct atgagcttca tatgagggta ggagacgagc gctgtaaaat
    16321 tggtagctat gattttgaaa agtttgcagc cgttatcagt cactttaagt ttgtggcggg
    16381 tatgaatgtg ggagaaaaaa gacgtagtca actgggttcc tgtgattatg cctatgacca
    16441 gaagatggcg tctctacgtt tatctactgt aggcgattat cgggggcatg agagtttggt
    16501 tatccgtttg ttgcacgatg aggagcagga cttgcatttt tggtttcagg atattgaaga
    16561 attaggcaag cagtacaggc aacggggact ctatcttttt gctggtccgg ttgggagtgg
    16621 taagacgacc ttgatgcatg aattgtccaa gtcactcttt aaaggacagc aagttatgtc
    16681 catcgaagat cctgtcgaaa tcaagcagga cgacatgctt cagttgcagt tgaacgaagc
    16741 aatcggccta acctatgaaa atctaatcaa actttccttg cgtcatcgac cagatctctt
    16801 gattatcgga gaaattcgtg acagcgagac ggcgcgtgca gtggtcagag ctagtttgac
    16861 aggtgcgaca gtcttttcaa ccattcacgc caagagtatc cgaggtgttt atgagcgtct
    16921 gctggagttg ggtgtgagtg aagaagaatt ggcagttgtt ctgcaaggag tctgctacca
    16981 gagattaatc gggggaggag gaatcgttga ctttgcaagc agagattatc aagaacacca
    17041 agcagccaag tggaatgagc aaattgacca gcttcttaaa gatggacata tcacaagtct
    17101 tcaggctgag acggaaaaaa ttagctacag ctaagcaaaa aaatatcatc accctattta
    17161 acaatctctt ttctagcggt tttcatctgg tggagactat ctccttttta gataggagtg
    17221 ctttgttgga caagcagtgt gtgatccaga tgcgtgcggg cttgtctcag gggaaatcat
    17281 tctcagaaat gatggaaagt ttgggatgtt caagtaccat tgtcactcag ttatctctag
    17341 ctgaagttca tggcaatctt cacctgagtt tgggaaagat agaagaatat ctagacaatc
    17401 tggccaaggt caagaaaaag ttaattgaag tagcgaccta tcctttgatt ttgctgggtt
    17461 ttcttctctt aattatgctg gggctacgga attacctgct cccacaactg gatagtagca
    17521 atattgccac ccgaattatc ggtaatctgc cacaaatttt tctaggcatg gtagggcttg
    17581 tttccgtgct tgccctttta gcactaactt tttataaaag aagttctaag atgagtgtct
    17641 tttctatctt agcacgcctt ccctttattg gaatctttgt gcagacctac ttgacagcct
    17701 attatgcacg tgaatggggg aatatgattt cacagggaat ggagctgacg cagatttttc
    17761 aaatgatgca ggaacaaggt tcccagctct ttaaagaaat cggtcaagat ctggctcaaa
    17821 ccctgaaaaa tggccgtgaa ttttctcaga cgataggaac ctatcctttc tttaggaagg
    17881 aattgagtct catcatagag tatggggaag ttaagtccaa gctgggtagt gagttggaaa
    17941 tctatgctga aaaaacttgg gaagcctttt ttacccgagt caaccgcacc atgaatttgg
    18001 tgcagccact ggtttttatc tttgtggcac tgattatcgt tttactttat gcggcaatgc
    18061 tcatgcccat gtatcaaaat atggaggtaa atttttaaaa tgaaaaaaat gatgacattc
    18121 ttgaaaaaag ctaaggttaa agcttttaca ttggtggaga tgttagtagt cttgctgatt
    18181 atcagcgtgc ttttcttgct ctttgtacct aatctgacca agcaaaaaga agcagtcaat
    18241 gacaaaggaa aagcagctgt tgttaaggtg gtggaaagcc aggcagaact ttatagcttg
    18301 gataaaaatg aagatgctac tctgagcaag ttacaaggag atgggcgcat cacggaagaa
    18361 caggttaaag cttataatga atactatact aaaaatggag gggcaaatcg taaagtcaat
    18421 gattaaggcc tttaccatgc tggaaagtct cttggttttg ggacttgtga gtatccttgc
    18481 cttgggctta tccagctctg tccagtccac ttttgcagca gtagaggagc agattttctt
    18541 tatggagttt gaagaactct atcgggaaac ccaaaaacgc agtgtagcca gtcagcaaaa
    18601 gactagtcta aatttagatg ggcagacgct tagcaatggc agtcaaaagt tgacagttcc
    18661 taaaggaatt caggcaccat caggccaaag tattacattt gaccgagctg ggggcaattc
    18721 gtccctggct aaggttgaat ttcagaccag taaaggagcg attcgctatc aattatatct
    18781 aggaaatgga aaaattaaac gcattaagga aacaaaaaat tagggcagtg attttactgg
    18841 aagcagtagt cgctctagct atctttgcca gcattgcgac cctccttttg ggacaaattc
    18901 aaaaaaatag gcaagaggaa gcaaaaatct tgcaaaagga agaagtcttg agggtagcta
    18961 agatggccct gcagacgggg caaaatcagg taagcatcaa cggagttgag attcaggtat
    19021 tttctagtga aaaaggattg gaggtctacc atggttcaga acagttgttg gcaatcaaag
    19081 agccataagg tcaaggcttt taccttgtta gaatccctgc ttgccctcat tgtcatcagt
    19141 gggggattac tcctttttca aactatgagt cagctcctca tttcagaagt tcgctaccag
    19201 caacaaagcg agcaaaagga gtggctcttg tttgtggacc aacttgaggc agaattagac
    19261 cgttcgcagt tcgaaaaagt agaaggcaat cgcctataca tgaagcaaga tggcaaggac
    19321 atcgccatcg gtaagtcaaa gtcagacgat tttcgtaaaa ccgatgctag tggacggggg
    19381 tatcagccta tggtttatgg cctcaaatca gctcagatta cagaggacaa tcaactggtt
    19441 cattttcgtt tccagtttca aaaaggctta gaaagggagt tcatctatcg tgtggaaaaa
    19501 gaagaaagtt aaggcaggtg ttctcctcta cgcagtcacc atagcagcca tctttagtct
    19561 tttgttgcaa ttttatttga accgacaaat cgcccactat caagactatg ctttaaataa
    19621 agaaaaattg gttgcttttg ccatggctaa acgaaccaag gataaagccg agcaagaaag
    19681 tggggaacag gtttttaatc taggtcaggt aagctatcaa aacaagaaaa caagcttggt
    19741 gacgactgtt cgtacgtcta agagccaata tgagtttctg tttccttcag tcaaaatcaa
    19801 agaagagaaa agagataaaa aggaagaggt agcgaccgat tcaagcgaaa aagcggagaa
    19861 aaaaaatcag aagagaagcc tgaaaagaaa gagaattcct agtcaattca actataatgc
    19921 gttgaatcca gaatagtcca ctgtagtttc tagaaaattg ctggaaatgg atgttaagct
    19981 ccaattcatt tgtttatatc ttatttcagt ttactatact ttgtgctaaa ctaaagatat
    20041 gaaacatgat tttaaccaca aagcagaaac tttcgactcc cctaaaaata tcttcctcgc
    20101 aaacttggta tgtcaagcag tcgagaaaca gattgatatt ttatcagaca aagtaatttt
    20161 agatttcggt ggtggcacgg gtctattagc cttgccccta gccaagcagg ctaagtcagt
    20221 tactcttgta gacatttctg agaaaatgtt ggagcaagct cgtttgaaag tggagcagca
    20281 agcaatcaag aatatccagt ttttggagca agatttaccg aaaaatccct tggagaaaga
    20341 gtttgattgc cttgctgtta gtcgggttct tcatcatatg cctgatttgg atgcggctct
    20401 ctcactgttt catcaacatt tgaaggaaga tgggaaactc atcattgctg attttaccaa
    20461 gacagaagct aatcatcatg gatttgattt agctgaactg gaaaacaagc taattgagca
    20521 tggtttttca tctgtgcata gtcagattct ctatagcgct gaagacctgt ttcaaggaaa
    20581 tcactcagaa ttctttttaa tagtagccca aaaatcactc gcctagtcag ggagtgattt
    20641 ttctataagg atggaaaaaa gaagggaaat ttgataagat aggaatatgg attttgaaaa
    20701 aattgaacaa gcttatacct atttactaga gaatgtccaa gttatccaaa gtgatttggc
    20761 gaccaacttt tatgacgcct tggtggagca aaacagcatc tatctggatg gtgaaactga
    20821 gctaaaccag gtcaaagaca acaatcaggc ccttaagcgt ttagcactac gcaaagaaga
    20881 atggctcaag acctaccagt ttctcttgat gaaggctggg caaacagaac ccttgcaggc
    20941 caatcaccag tttacaccag atgccattgc tttgcttttg gtgtttattg tggaagagtt
    21001 gtttaaagag gaggaaatta ctatcctcga aatgggttct gggatgggaa ttctaggcgc
    21061 tactttcttg acctcgcttg ataaaaaggt ggattacttg ggaatggaaa tggatgattt
    21121 gctgattgat ctggcagcta gcatggcaga tgtaattggt ttgcaggctg gctttgtcca
    21181 aggagatgcc gttcgtccac aaatgctcaa agaaagcgat gtggtcatca gtgacttgcc
    21241 tgttggctat tatcctgatg atgccgttgc gtcgcgccat caagttgctt ctagccaaga
    21301 acatacttac gcccatcact tgctcatgga acaagggctt aagtacctca agtcagacgg
    21361 atacgctatt tttctagctc cgagtaattt gttgaccagt cctcaaagtg atttgttaaa
    21421 agaatggctg aaagaagagg cgagtctggt tgctatgatt agtctgcctg aaaatctctt
    21481 tgctaatgcc aaacaatcta agactatttt tatcttacag aagaaaaatg aaatagcagt
    21541 agagcctttt gtttatccac ttgctagctt gcaagatgca agtgttttaa tgaaatttaa
    21601 agaaaatttt caaaaatgga ctcaaggtac tgaaatataa aatagatttt gttataatag
    21661 ttgaaaacgc ttaaaaaggg gtatcatgtt atgacaaaaa caattgcaat caatgcagga
    21721 agttcaagtt tgaaatggca attatactta atgccagaag aaaaagtgtt agccaaaggt
    21781 ttgattgaac gtatcggttt gaaagattca atttcaactg taaaatttga cggccgttct
    21841 gaacaacaaa ttttggatat tgaaaatcat acacaagccg ttaaaatttt attggatgac
    21901 ttgattcgtt tcgatattat caaggcttat gacgagatta caggtgttgg acatcgtgtt
    21961 gttgctggtg gagaatattt caaagaatca acagttgttg agggagatgt tttagaaaaa
    22021 gttgaagagt tgagtttgtt ggctcctcta cacaacccgg ccaatgcagc aggtgttcgt
    22081 gccttcaagg aattgttgcc agacattacc agtgtagttg tttttgatac ttccttccac
    22141 acaagtatgc cagagaaagc ttatcgctac cctctaccaa caaaatatta cacagaaaac
    22201 aaggttcgta aatacggtgc tcatggtaca agtcaccagt ttgtagcagg agaagctgca
    22261 aaactcttgg gacgtccatt agaagacttg aagttaatta cctgtcatat tggtaacgga
    22321 ggctcaatta cagctgtgaa agccggcaaa tctgtagaca cttctatggg gttcactcct
    22381 cttggtggta ttatgatggg aacgcgtaca ggggatattg atccagctat cattccttat
    22441 ttaatgcaat atacagagga ttttaacaca ccagaagata tcagtcgtgt tcttaaccgt
    22501 gaatcaggtc ttttgggagt ttctgctaat tctagcgata tgcgcgatat agaagcagct
    22561 gtagcagaag ggaatcacga ggctagcttg gcttatgaaa tgtatgttga ccgtatccaa
    22621 aaacatatcg gtcagtactt tgcagtgcta aatggagcag atgccattgt tttcacagca
    22681 ggtgtcggtg aaaatgcaga gaatttccgt cgtgatgtaa tctcagggat ttcgtggttt
    22741 ggttgtgatg ttgatgatga aaagaatgtc tttggcgtta caggagacat ctcaacagag
    22801 gcagctaaaa tccgtgtctt ggttattcca acagatgaag aattagtcat tgcccgtgac
    22861 gttgaacgct tgaaaaaata agtgaaacta aaaaaatatt caatacaagg agttgggaaa
    22921 gttatttttc cagcttcttt ttctgatgaa attgtccaaa accttgctat gattggcttt
    22981 tttgaaaaat atggtataat agtagtaatt taatagatgg agttgagttt tgaagaaaaa
    23041 ctttcgtgta aaaagagaga aagattttaa ggcgattttc aaggagggga caagttttgc
    23101 taatcgcaaa tttgtggtct accaattaga aaaccagaaa aaccattttc gagtaggtct
    23161 atcagttagc aaaaaactgg ggaatgccgt cactagaaat caaattaagc gacggattcg
    23221 gcatattatc cagaatgcaa aagggagtct ggtagaagat gtcgactttg ttgtcattgc
    23281 tcgaaaagga gtcgaaacct tgggatacgc agagatggag aaaaatctac tccatgtatt
    23341 aaaattatca aagatttacc gggaaggaaa tgggagtgaa aaagaaacta aagttgacta
    23401 gtttgctagg actgtctctg ttaatcatga cagcctgtgc gactaatggg gtaactagcg
    23461 atattacagc cgaatcggct gatttttgga gtaaattggt ttacttcttt gcgaaaatca
    23521 ttcgcttttt atcgtttgat attagtatcg gagtggggat tattctcttt acggtcttga
    23581 ttcgtacagt cctcttgcca gtctttcagg tgcaaatggt ggcttctagg aaaatgcagg
    23641 aagctcagcc acgcattaag gcgcttcgag aacaatatcc aggtcgagat atggaaagca
    23701 gaaccaaact agagcaggaa atgcgtaaag tatttaaaga aatgggtgtc agacagtcag
    23761 attctctttg gccgattttg attcagatgc cggttatttt ggccttgttc caagccctat
    23821 caagagttga ctttttaaag acaggtcatt tcttatggat taaccttggt agtgtggata
    23881 caacccttgt tcttccgatt ttagcagcag tattcacctt tttaagtact tggttgtcca
    23941 acaaagcttt gtctgagcga aatggcgcta cgactgcgat gatgtatggg attccagtct
    24001 tgatttttat ctttgcagtt tatgcgccag gtggagtcgc cctatactgg acagtgtcta
    24061 atgcttatca agtcttgcaa acctatttct tgaataatcc attcaagatt atcgcagagc
    24121 gcgaggccgt agtacaggca caaaaagatt tggaaaatag aaaaagaaaa gccaagaaaa
    24181 aggctcagaa aacgaaataa ataaggagga atctggtaat ggtagtattt acaggttcaa
    24241 ctgttgaaga agcaatccag aaaggattga aagaattaga tattccaaga atgaaggctc
    24301 atatcaaagt catttctagg gagaaaaaag gctttcttgg tctatttggt aaaaaaccag
    24361 cccaagtgga tattgaagcg attagtgaaa cgactgttgt caaagcaaat caacaggtag
    24421 taaaaggcgt tccgaaaaaa atcaatgatt tgaacgagcc tgtgaagacg gttagtgaag
    24481 aaaccgttga ccttggtcat gtggttgatg ctattaaaaa aatagaggaa gaaggtcaag
    24541 gtatttctga tgaagtcaag gctgaaatct taaaacatga aagacatgcc agcactatct
    24601 tagaagaaac tggtcacatt gagattttaa atgaacttca aatcgaggaa gcgatgaggg
    24661 aagaagcagg cgctgatgac cttgaaactg agcaagatca aactgaaaat caagacttga
    24721 aagagatggg cttgaaggtc gagcaaagtt atgatattgc ccaggtggct acggatgtga
    24781 ctgcctatgt tcaagcgatt gtggatgaca tggatgttga agctacactt tcaaatgatt
    24841 ataaccgtcg tagcatcaat ctacaaattg acaccaacga accaggtcgt attatcggct
    24901 accatggtaa agtcttgaag gccttgcaac tgttggctca aaattatctt tacaaccgct
    24961 attccaaaac cttctacgtt acaatcaatg tcaatgatta tgtcgaacac cgtgcagaag
    25021 tcttgcagac ctatgcgcaa aaattggcga ttcgtgtttt agaagaagga cgtagtcatc
    25081 aaacagatcc aatgtcgaat agtgaacgca agattatcca ccgtattatt tcacgtatgg
    25141 atggcgtgac cagttactct gaaggtgatg agccaaatcg ctatgtcgtt gtagatacag
    25201 aataagtaaa atcagggttg acctgatttt ttgctagcta gaggaggtta aactgatgtt
    25261 gaataagata agagactatt tagactttgc tggtttgcag taccgtaatc ctgataaagc
    25321 gggagcagag cgagagaaga tgctggcatt ccgccacaaa ggacaagagg cccgaaaggc
    25381 ttttacagaa ctggccaaag cctttcaagc aagccatcca gaatggcaac tccaacagac
    25441 tagtcagtgg atgaatcagg cccagcgttt gagaccacat ttttgggttt atctacagag
    25501 agacggacaa gtgacagaac ctatgatggc cttacgtttg tatgggacat ctactgactt
    25561 tggaatttct ttggaagtca gtttcatcga acgtaagaag gatgagcaaa cactgggcaa
    25621 gcaggccaaa gttttagaca ttccaaccgt taaagggatt tattatctaa cctactctaa
    25681 tggtcaaagt caacggtggg aggcgaatga agaaaagcgt cgtactttac gcgagaaggt
    25741 gagaagtcaa gaagttcgaa aagttttagt gaaggtagat gttcctatga cagaaaattc
    25801 gtctgaagaa gaaatcgtag aaggcttatt gaagtcttat tctaaaattc ttccctatta
    25861 tctagctacg agaaaataag ataatttgta aaacatcata aatcatacag tccaagagtg
    25921 aacagtccgc tgtgtaattc ttggtctttt tgtttgcgct ttcgcattat ataataaact
    25981 tacaaaaaca attcaaaagg agaacaatta tggaagtcgt ttcaagtgtt ctaaattggt
    26041 tttctagcaa tattttgcag aatcccgcat ttttcgtagg tttattggtg ttgataggat
    26101 atgcactttt gaaaaaacct gcccatgacg ttttttcagg gtttgttaaa gcaacagtag
    26161 ggtatatgtt gcttaacgtg ggtgctggtg gtttggttac aacctttcgt ccaatcttag
    26221 cagctcttaa ctacaaattc caaattggtg cagcggttat cgacccttac tttggacttg
    26281 ctgcagcaaa caacaaaatt gcagcagagt ttccagattt tgttggaact gcaactacag
    26341 ctctattgat tggttttgga ataaatatct tgctcgtagc tcttcgaaag attacgaagg
    26401 taagaaccct ctttattact ggtcacatca tggtacaaca agctgcaaca gtatctctta
    26461 tggttctatt cttagtacca caattgcgca atgcttacgg tacagcagcg attggtatca
    26521 tctgtggact ttactgggca gttagttcaa atatgactgt tgaggcaact caacgcttga
    26581 ctggtggtgg cggatttgcg attggtcacc aacagcaatt tgcaatctgg tttgtagata
    26641 aagtagcagg acgctttggt aagaaagaag aaagtttaga caatcttaaa ttacctaagt
    26701 tcctctcaat cttccacgat acagttgttg catctgctac cttgatgctc gtattcttcg
    26761 gggccattct tttaatcttg ggtccagaca ttatgtctaa taaagaagtc atcacttcag
    26821 gaactctatt caatcctgct aaacaagatt tctttatgta cattatccaa acagccttta
    26881 ccttctcagt ttacttgttc gttttgatgc aaggtgtccg aatgttcgta tctgagttga
    26941 caaacgcctt ccaaggtatt tcaaacaaat tgttgccagg ttcattccca gcggttgacg
    27001 ttgcagcttc ttatggattt ggttctccaa atgctgtctt gtcaggattt acctttggtt
    27061 tgattggtca attgattaca attgtcttgc tcatcgtctt taaaaatccg attcttatta
    27121 ttacaggatt tgtaccagtg ttctttgaca atgcagccat tgcggtctac gctgataaac
    27181 gcggcggatg gaaagcggct gttatccttt cctttatatc aggtgtcctt caagttgctc
    27241 taggagctct ttgtgtggcc cttctcgatt tggcatctta tggtggctac catggaaata
    27301 tcgactttga attcccatgg cttggatttg gatatatctt caaatacctt ggtattgttg
    27361 gttatgtact tgtgtgtctc ttcttgcttg ttattcctca acttcaattt gccaaagcaa
    27421 aagataaaga gaaatattac aacggtgaag ttcaagaaga agcttagtat ctagaaaagg
    27481 agaaataaaa tggttaaagt attagcagcg tgcggaaatg gaatgggttc atcaatggtt
    27541 atcaagatga aggttgaaaa tgctctccgt aagcttaatc aaacagattt tacagtcaat
    27601 tcatgcagtg tcggtgaagc taaaggttta gcagtaggat atgacatcgt aatcgcttct
    27661 cttcatttga ttcaagaatt ggaagggcga actaatggga agttaattgg acttgataac
    27721 ttgatggatg ataaagaaat caccgaaaaa ctcagtcaag cactacagta aaaggttgga
    27781 gggggctgga cagaaactga gagttatcgt ttctgtcctt ctccctcttt aaataaagga
    27841 ggcagatatg aatttaaaac aagctttaat tgacaatgac tcgatccgac taggtttaga
    27901 ggctaacaat tggaaagaag cagtcaaggt agcagtagat cccttaattg aaagtggggc
    27961 aattttgcca gagtattacg atgctatcat tgaatcgact gaagagtatg ggctttacta
    28021 tatcttgatg ccaggtatgg ctatgcccca cgctagacct gaagctggtg tgcaaagtga
    28081 tgccttttca ttgattacct tacaaaatcc tgttgtattt tcagatggga aagaggtatc
    28141 tgttttgttg gcactagcag caacaagctc aaaaattcac acaagtgtag ccattccaca
    28201 aattattgcc ctgtttgaat tagaagattc tattgcacgt ttacaggctt gccagactaa
    28261 agaagatgtc ttggctatga ttgaagaatc taaggatagc ccttatctcg aaggattgga
    28321 tttggaaagt tagaaagagg aataaagaaa tgacaaaaag aatacctaat ttacaagttg
    28381 cattagacca ttcagacttg caaggagcga ttaaagcagc tgtttctgtt ggtcaggaag
    28441 tagatattat cgaagctgga actgtttgct tgcttcaagt tggaagtgaa ctggctgaag
    28501 tcttgcgtag ccttttccca gataagatta ttgtggcaga cacaaaatgt gctgatgctg
    28561 gtggaacagt tgctaaaaat aatgcggttc gtggagcaga ctggatgact tgtatctgtt
    28621 gtgcaaccat ccctactatg gaagcagctc taaaggctat caagactgaa cgaggagaac
    28681 gaggcgaaat ccagatcgag ctttatggcg attggacttt tgaacaagct cagctttggc
    28741 tagatgcagg tatctcacaa gctatttatc accaatctcg tgatgctctt cttgctggtg
    28801 aaacttgggg tgaaaaagac cttaataagg ttaaaaaact cattgacatg ggcttccgtg
    28861 tatctgtaac aggtggtcta gatgtagata ctctcaaact ctttgaaggt gttgatgtct
    28921 ttacctttat cgcaggtcgt ggaattacag aggctgcgga tccagcagga gcagcgcgtg
    28981 ccttcaagga tgaaatcaaa cgaatttggg ggtaaatcat ggtacgtcca attggaattt
    29041 atgaaaaggc aaccccaaca cactttactt ggctagaacg tttaaatttt gccaaggagt
    29101 taggctttga ttttgtcgag atgtctattg acgaacgtga cgagcgttta gcaagacttg
    29161 actggagtaa ggaagaacgc ttggaagttg tcaaagcaat ctatgaaact ggtgttcgta
    29221 ttccttctat ctgtttttca ggccatcgtc gctacccatt gggttcaaaa gatccagttc
    29281 tagaggaaaa atctctagaa ctcatgaaaa aatgtatcga attagctcaa gacttgggag
    29341 ttcgtacgat tcaattagct ggttacgatg tttactatga ggaaaagtca ccccagacac
    29401 gccaacgttt tatcaaaaat ttgagaaaag cctgtgactg ggctgaagaa gctcaggtgg
    29461 tacttgctat tgaaattatg gatgatcctt tcatcaatag catcgaaaaa tatttggcta
    29521 tagaaaaaga gattgactct cccttcctct ttgtatatcc agatattggt aatgtgtctg
    29581 catggcataa tgatatctat agtgagtttt atcttggtca tcatgccatc gcagctctcc
    29641 atctcaagga tacttatgca gtgacagaaa gttcaaaggg ccagttccga gatgtacctt
    29701 tcgggcaagg ttgtgtcaaa tgggaagaag ctttcgatat tttaaaggaa accaattata
    29761 atggaccttt cctaatcgaa atgtggtctg aaaattgtga aacagtagaa gaaacacgcg
    29821 cagccattca agaggcgcaa gcttttctct atccactcat taagaaagca ggtttgatgt
    29881 aagatgaatc aagtaatcaa tgctatgcgt aaacgagtct gtgatgccaa tcaatcattg
    29941 ccaaaacatg gacttgtcaa atttacctgg gggaatgtat ctgaagttaa tcgcgaactc
    30001 ggtgtcattg ttatcaaacc atcaggcgtg gattatgacg aattgacacc tgaaaacatg
    30061 gtagtgactg atctagatgg taagatccta gaaggggatt taagaccatc ttccgacctc
    30121 ccaactcatg tgcaattata taaggcttgg tcagaaattg gtagtgtggt tcacacccat
    30181 tcgacagaag ctgttggttg ggctcaggca ggtcgtgata ttcctttcta cggaacaacc
    30241 catgcagatt atttctacgg ttcaatccct tgcgcccgta gtttgaccaa ggacgaagta
    30301 gaagtggcct atgaaaaaga tactggcctg gttatcgtag aagagtttga acatcgcgga
    30361 cttaacccgg ttgaagtacc aggaattgtt gtacgcaatc acggtccatt cacctggggc
    30421 aaaaatccag agaatgctgt ttatcactct gtcgtactag aggaagtatc aaagatgaat
    30481 cgctttacag aacaaatcaa tccaagagtt ggacctgctc cccagtacat actagaaaaa
    30541 cactaccaac gtaaacatgg accaaatgct tattatggtc aaaagtaaga acgatgaagg
    30601 aggagaaaaa gataaattta gctcctcttt ttacatttga tttttattga gagtaaagtt
    30661 ggaattgaag taattttaaa agatttttta gaaatagcgc ttgatatata tatggtaaaa
    30721 taaaaagaat tgctgtgata tcaatagatt tgggggattt tttaatatgg tactggataa
    30781 ggcaagttgt gatttgcttc aatatttgat ggatcaagaa acgtccaaaa cgattatggc
    30841 gatttcgaaa gatttgaaag agtcaagaag gaaaatttat tatcacattg acaaaatcaa
    30901 tgctgctctg gttgacgagg cgcttcacat cattagtatt ccacgaattg gtattcactt
    30961 aacggaagag cagagagatg cttgttgtaa actattatcg gaagtagatt cgtacgatta
    31021 tatcatgagt gcgcatgaac gtatgatgat aatgttacta tggataggta tttctaaaga
    31081 acgtattacg attgaaaaat tgatagagtt aacagaggta tctaggaata ctgttctcaa
    31141 tgatttgaat agtattcgtt atcaactaac tttggaacaa tatcaggtga tcttgcaagt
    31201 gagcaagtca cagggatacc accttcatgc ccaccctctt aataaaattc agtatcttca
    31261 atcgcttcta tatcatattt ttatggaaga aaatgccact tttgtatcta ttttagaaga
    31321 taagatgaaa gagaggttag atgatgagtg tttgctttct gttgaaatga accaattttt
    31381 taaggaacag gttcctttag ttgaacaaga tttagggaag aaaataaacc atcatgaaat
    31441 aacttttatg ttgcaggttc taccttattt gctgttaagc tgtcataatg ttgaacagta
    31501 tcaagaaaga catcaggata tagagaaaga attttctttg ataagaaaaa gaatagagta
    31561 tcaggtgtct aagaaattag gagaacggtt gtttcaaaag tttgaaattt ctttgtcagg
    31621 acttgaagtt tctcttgtag ctgttctcct cctctcctat cgtaaggatt tggatattca
    31681 tgcagaaagt gatgattttc ggcaattaaa acttgcttta gaagaattta tctggtattt
    31741 tgaatcacaa atccgaatgg agattgagaa caaggatgat ttgttacgaa atttgatgat
    31801 ccactgtaaa gccttgttat ttagaaagac ttacggtatt ttttctaaaa atcctctaac
    31861 aaaacaaatt cgatccaagt atggagaatt atttttagtc actagaaaat ctgcggaaat
    31921 tttagaagga gcatggttta ttcggctaac agacgatgat attgcctatt tgacgattca
    31981 tattggagga tttttaaagt atacaccatc gtctcaaaaa aatatgaaaa aagtttatct
    32041 cgtttgtgat gaaggtgttg cggtttcgag acttttgctg aaacaatgca aactttattt
    32101 tccaaatgag caaattgaca ctgtatttac aacagaacaa tttaagagtg tggaagatat
    32161 tgcacaagtt gatgtagtga ttactactaa tgatgatttg gatagcagat ttccgatttt
    32221 aagggttaat cctatccttg aagcagaaga tattttgaaa atgctagact atcttaaaca
    32281 caatatattt cgtaataaga gcaaaagttt cagtgaaaat ctttctagtc ttatttcgtc
    32341 ttatattgta gacagcaagt tggctagtaa gttccaagaa gaggttcaaa cacttataaa
    32401 taaagaaata gtagttcaag cttttttgga agatatttga aggacagtcc aatgatgaac
    32461 acaaacctgt gtttttcttg gtctttttta gtgttttgaa gggtggtata ctaatctcaa
    32521 agataacaat tatatccaaa ggaggcaaca tatgccaaac gtcaaagaaa ttacaagaga
    32581 gtcatggatt ttagccactt tcccagagtg gggaacatgg ttgaacgaag aaatcgaaga
    32641 agaagtcgta cctgaaggca actttgccat gtggtggcta ggcaactgtg gtacttggat
    32701 taagacacca gctggtgcta acgttgtcat ggacctttgg tcaaaccgtg gaaaatcaac
    32761 caaaaaagtg aaagatatgg ttcgtgggca ccaaatggca aatatggcag gtgttcgtaa
    32821 gttgcaacca aacttgcgtg ttcagccaat ggttatcgat ccatttgcta tcaacgaact
    32881 agactattac ttagtttcac acttccacag tgatcatatc gacccataca cagctgcagc
    32941 aattctcaac aatcctaagt tagagcatgt taagtttatc ggtccttacc actgtggacg
    33001 aatctgggaa ggatggggtg ttccaaaaga acgtatcatc gttgttaaac caggtgacac
    33061 tatcgaatta aaagatatga agattcatgc agtagaatca tttgaccgta cttgcttggt
    33121 aactctccca gtgaacggtg ctgatgagac aggcggtgaa cttgctggct tggctgttac
    33181 agatgaagaa atggctcaaa aggctgttaa ctatatcttt gaaacaccag gtggaaccat
    33241 ctatcatggt gcagattctc acttctcaaa ctattttgca aaacatggta aagactttaa
    33301 aattgatgtt gctttgaata actatggtga aaatccggta ggtatccaag acaaaatgac
    33361 atctatcgat cttcttcgta tggcagaaaa tctgcgtacc aaagtcatta tcccagttca
    33421 ctatgatatc tggtctaact tcatggcttc tactaatgag attctagaac tttggaaaat
    33481 gcgaaaagat cgcttgcaat acgatttcca tccatttatc tgggaagttg gcggtaagta
    33541 cacttatcct caagatcaac acttagtaga ataccatcat ccacgtggtt ttgatgattg
    33601 ttttgaacaa gactctaaca ttcaatttaa agctttgcta taatataaaa atattctttg
    33661 gaggttatcc gtatgttgca tgatacggat aattttcata taatagtaaa atagaatgtg
    33721 tgattcaata atcacctcaa atagaaagga aattctatgt caaatctatc tgttaatgca
    33781 attcgttttc taggtattga cgccattaat aaagccaact caggtcatcc aggtgtggtt
    33841 atgggagcgg ctccgatggc ttacagcctc tttacaaaac aacttcatat caatccagct
    33901 caaccaaatt ggattaaccg cgaccgcttt attctttcag caggtcatgg ttcaatgctc
    33961 ctttatgctc ttcttcacct ttctggtttt gaagatgtca gcatggatga gattaagagt
    34021 ttccgtcaat ggggttcaaa aacaccaggt cacccagaat ttggtcatac ggcagggatt
    34081 gatgctacga caggtcctct agggcaaggg atttcaactg ctactggttt tgcccaagca
    34141 gaacgtttct tggcagccaa atataaccgt gaaggctaca atatctttga ccactatact
    34201 tacgttatct gtggagacgg agacttgatg gaaggtgtct caagcgaggc agcttcatac
    34261 gcaggtttgc aaaaacttga taagttggtt gttctttatg attcaaatga tatcaacttg
    34321 gatggtgaga caaaggattc ctttacagaa agtgttcgtg accgttacaa tgcctacggt
    34381 tggcatactg ccttggttga aaatggaaca gacttggaag ccatccatgc tgctatcgaa
    34441 acagcaaaag cttcaggcaa gccatcttta attgaagtga agacggttat tggatatggt
    34501 tctccaaaca aacaaggaac taatgctgta cacggcgccc ctcttggagc agatgaaacc
    34561 gcatcaactc gccaagccct cggttgggac tacgaaccat ttgaaattcc agaacaagta
    34621 tatgctgatt tcaaagaaca tgttgcagac cgtggcgcat cagcttatca agcttggact
    34681 aaattagttg cagattataa agaagctcat ccagaactgg ctgcagaagt agaagccatc
    34741 atcgacggac gtgatccagt cgaagtgact ccagcagact tcccagcttt agaaaatggt
    34801 ttttctcaag caactcgtaa ttcaagtcaa gatgccttga atgttgtggc agctaaatta
    34861 ccaaccttcc taggtggatc agctgacctt gctcactcaa acatgactta tatcaagacg
    34921 gatggacttc aagacgatac caatcgcttg aatcgtaaca ttcagtttgg tgttcgtgaa
    34981 tttgcaatgg gaacaatctt gaacgggatg gcccttcatg gtggacttcg tgtatacggt
    35041 ggaactttct tcgtcttctc tgactatgtg aaggcagctg tccgcttgtc agccttacaa
    35101 ggacttcctg tgacttatgt ctttacccat gattcaatcg cagttgggga agatggtccg
    35161 actcacgaac cagttgagca tttagcaggt cttcgtgcta tgccaaatct aaatgttttc
    35221 cgtccagcag atgcgcgtga aacgcaagca gcttggtacc ttgcagtgac aagtgagaaa
    35281 acaccaactg cccttgtctt gacacgtcaa aacttgactg ttgaagatgg aacagacttc
    35341 gacaaggttg ctaaaggtgc ttatgttgta tatgaaaatg cagccgactt tgataccatc
    35401 ttgattgcga caggttcaga ggttaatctt gctgtctcag ctgctaaaga attggctagt
    35461 caaggcgaaa aaatccgcgt agtcagcatg ccatctacag atgtctttga taaacaagat
    35521 gcagcttaca aggaagagat tcttccaaat gcagtccgcc gtcgtgttgc agtcgaaatg
    35581 ggtgcaagtc aaaactggta caaatatgtt ggtctcgatg gtgccgttct aggtattgat
    35641 accttcggag cctctgcccc agcaccaaaa gtattggcag aatatggctt tactgtagaa
    35701 aatcttgtaa aaattgttcg aaacttgaaa taatcctaaa aatcagggcg taagctctgg
    35761 tttttcttac cagaaaagta aggtacaatc ttgtaaaagt agctgaaatt tgatatagta
    35821 gtcctatgta aaagacaaag gagaaaacaa tgaatccaaa tattactttt ttaatcatgc
    35881 ttgtaggtat gatggccttg atgttcttta tgcaacgttc tcaaaagaaa caagctcaaa
    35941 agcgtatgga aagcttaaat aaactacaaa aaggctatga agtgattaca atcggtggac
    36001 tttatggaac agtcgatgaa gtagatacgg agaagggaac aatagttctt gacgtagatg
    36061 gagtttactt gacttttgaa ctagctgcta tcaagacagt attaccgctg aaagaaacag
    36121 cttcactaga aggcgcaatt gaaaaataag acgggatcac gaactcccgt ttttctataa
    36181 aagaaaggaa atgggatgaa aaaattagtc tttgtctgtc tgggaaatat ttgccgtagc
    36241 cctatggccg agtttgttat gaaatcaatg acagataact acgaaatcca aagtcgagca
    36301 acttcctctt gggaacatgg caatccgatt cataagggga ctcagggaat ttttcaagag
    36361 tatgagattc cttatgacaa gaacaagaca tcgcttcaga ttagtaagga agattttgaa
    36421 gcctttgatt atattatcgg aatggacgct tcaaatattt ctgacttatg tcagatgtgt
    36481 ccagtagact gtcaagataa gatttactca ttttcatctg aaagtgttcc agacccttgg
    36541 tatacaggag attttgaaga aacctatcga cgtgttcaag agggctgtca agcttggtta
    36601 gaacgtttag aaaaggagag ttaaggtgga aaatcttacg aatttttacg aaaagtatcg
    36661 tgtctatctg actcgtccac gtttagagct tttggcagta gttaccattg ttttttgtgc
    36721 tgtactcgtc ttttttctaa atattccagg aaaaggtgtc ttaaaactcg ataatggaac
    36781 gattgtttat gatggcagtc ttgtccgtgg taaaatgaat ggccaaggta ccattacctt
    36841 ccaaaatgga gaccaatata caggtggctt caacaatgga gccttcaacg gaaaaggtac
    36901 ctttcaatct aaagaaggct ggacctacga aggtgatttt gtaaatggtc aggctgaagg
    36961 aaaagggaaa ctaacaacag aacaagaagt cgtttatgaa ggaactttta aacaaggcgt
    37021 ttttcaacaa aaataaagcc tccttatcaa aggaggtatt attagaatca caaggtaagc
    37081 gcttacctgt aaatcccttt ctttccaaat ccctcttcca agcaagtttg tgaaataaaa
    37141 aatatttgaa ataaatttca caaacttcaa agataaaact tgataagaaa agaaaatgag
    37201 aaaagtttca caagagttta aaaattcttt gtgaaatgtt tatgaaactg tttacaaagt
    37261 tgaaataatg cgttataata aacttgtgaa aaaattaaca aaggatatcg ttccttgaaa
    37321 gctatggagg aaaatatggc tgataaaaaa actgtgacac cagaggaaaa gaaactcgtt
    37381 gctgaaaaac acgtagatga gttggttcaa aaagctctag ttgcccttga agaaatgcgt
    37441 aaattggatc aagaacaagt tgactacatc gttgccaaag catcagtagc agctttggat
    37501 gcccacggag aattggcttt acatgccttt gaagaaacag gacgtggtgt atttgaagac
    37561 aaagcaacta agaacttgtt tgcttgtgaa cacgtagtaa acaatatgcg ccacactaag
    37621 acagttggcg ttatcgaaga agacgatgta acaggattga ctcttattgc tgaaccagtt
    37681 ggtgttgttt gtggtattac tccaacaaca aacccaacat caacagcaat cttcaaatca
    37741 ttgatttcat tgaagacacg taacccaatc gtctttgcct tccatccatc agcacaagaa
    37801 tcatctgctc atgcagctcg tatcgtccgc gatgcagcta tcgcagctgg tgctcctgaa
    37861 aactgtgtgc aatggattac tcaaccatct atggaagcaa caagtgccct tatgaaccac
    37921 gaaggtgttg cgacaatcct tgcaacaggt ggtaatgcca tggttaaggc ggcttattca
    37981 tgtggtaaac cagctcttgg ggtaggtgcc ggaaacgttc cagcttatgt tgaaaaatca
    38041 gcaaacattc gtcaagcagc acacgatatc gtcatgtcta aatcatttga taacggtatg
    38101 gtctgtgcat ctgaacaagc agttatcatt gataaagaaa tttacgatga atttgtagca
    38161 gagttcaaat cttaccacac ttactttgta aacaaaaaag aaaaagctct tcttgaagag
    38221 ttctgcttcg gcgtcaaagc aaacagcaaa aactgtgctg gtgcaaaatt gaacgctgac
    38281 atcgttggta aaccagcaac ttggattgca gaacaagcag gatttacagt tccagaagga
    38341 acaaacattc ttgctgcaga atgtaaagaa gttggcgaaa atgagccatt gactcgtgaa
    38401 aaattgtcac cagttattgc agttttgaaa tctgaaagcc gtgaagatgg tattactaag
    38461 gctcgtcaaa tggttgaatt taacggtctt ggacactcag cagctatcca cacagctgac
    38521 gaagaattga ctaaagaatt tggtaaagct gttaaagcta ttcgtgttat ctgtaactca
    38581 ccttctactt ttggtggtat cggggacgtt tacaatgcct tcttgccatc attgacactt
    38641 ggatgtggtt cttacggacg caactcagtt ggggataacg ttagtgccat taacctcttg
    38701 aatatcaaaa aagtcggaag acggagaaat aacatgcaat ggatgaaact tccttcaaaa
    38761 acatactttg aacgtgattc aattcaatac cttcaaaaat gtcgtgacgt tgaacgtgtc
    38821 atgatcgtta ctgaccatgc catggtagag cttggtttcc ttgatcgtat catcgaacaa
    38881 ctggaccttc gtcgcaataa ggttgtttac caaatctttg cggatgtaga accggatcca
    38941 gatatcacaa ctgtaaaccg tggtactgag attatgcgtg ccttcaaacc agataccatc
    39001 atcgcactcg gtggtgggtc tccaatggat gctgccaaag taatgtggct cttctacgag
    39061 caaccagaag tggacttccg tgaccttgtc caaaaattca tggatatccg taaacgtgcc
    39121 ttcaagttcc cattgcttgg taagaagact aaattcatcg cgattccaac tacatctggt
    39181 acaggatctg aagtaacacc atttgccgtt atctctgata aagcaaacaa ccgtaaatac
    39241 ccaatcgctg actactcatt gacaccaact gtggcaatcg tagatcctgc tttggtattg
    39301 acagttccag gatttgttgc tgctgatact ggtatggacg tattgactca cgcgacagaa
    39361 gcatacgtat cacaaatggc tagtgactac actgatggtt tagcacttca agccattaaa
    39421 ttggtctttg aaaatctcga aagctcagtt aagaatgcag acttccactc acgtgagaaa
    39481 atgcataacg cttcaacaat cgctggtatg gcctttgcca atgccttcct aggtatttct
    39541 cactcaatgg cccataagat tggtgcgcaa ttccacacaa tccacggtcg tacaaatgct
    39601 atcttgcttc catacgttat ccgttacaac ggtacacgtc cagctaagac agcaacatgg
    39661 cctaagtaca actactaccg tgcagatgaa aaataccaag atatcgcacg catgcttgga
    39721 cttccagctt ctactccaga agaaggggtt gaatcttacg caaaagctgt ctacgaactc
    39781 ggtgaacgta ttgggatcca aatgaatttt agagaccaag gaattgacga aaaagaatgg
    39841 aaagaacatt ctcgtgaatt agccttcctg gcttatgaag accaatgttc accagctaac
    39901 ccacgtcttc caatggtaga ccatatgcaa gaaatcatcg aagatgcata ctatggctac
    39961 aaagaaagac caggacgccg taaataattg tttatcagtc tagaagcaag acaaaaactc
    40021 aatttgaggg aaagatccag taatttttct atgataaaag gcatcctatc aaggtttttg
    40081 aacacctgat aggatgcctt tttatgatat tgaggccttt ttgccctttt tgaaaaacta
    40141 gaatagaaac aaaatatata atagattgaa actggaatag tacacttctg cttctaaaac
    40201 attgttagaa ttcgatttga ctgtcctgat cgatttg
//
LOCUS       DAVLVF010000010        38255 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVLVF010000010 DAVLVF010000000
VERSION     DAVLVF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38255
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.010"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            157..1182
                     /gene="trpS"
                     /locus_tag="VYN66_000488"
     CDS             157..1182
                     /gene="trpS"
                     /locus_tag="VYN66_000488"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV1494554.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1334..2812
                     /gene="guaB"
                     /locus_tag="VYN66_000489"
     CDS             1334..2812
                     /gene="guaB"
                     /locus_tag="VYN66_000489"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV1494555.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2863..3960)
                     /gene="recF"
                     /locus_tag="VYN66_000490"
     CDS             complement(2863..3960)
                     /gene="recF"
                     /locus_tag="VYN66_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV1494556.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKASVNGN"
     gene            complement(3963..4331)
                     /gene="yaaA"
                     /locus_tag="VYN66_000491"
     CDS             complement(3963..4331)
                     /gene="yaaA"
                     /locus_tag="VYN66_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV1494557.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            4490..5740
                     /locus_tag="VYN66_000492"
     CDS             4490..5740
                     /locus_tag="VYN66_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1494558.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYCSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            5737..7020
                     /locus_tag="VYN66_000493"
     CDS             5737..7020
                     /locus_tag="VYN66_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1494559.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSIDTLVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            7062..7883
                     /locus_tag="VYN66_000494"
     CDS             7062..7883
                     /locus_tag="VYN66_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1494560.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKISFLPLFYFIFFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7894..8439
                     /gene="pgsA"
                     /locus_tag="VYN66_000495"
     CDS             7894..8439
                     /gene="pgsA"
                     /locus_tag="VYN66_000495"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV1494561.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8436..9263
                     /locus_tag="VYN66_000496"
     CDS             8436..9263
                     /locus_tag="VYN66_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1494562.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            9248..10087
                     /locus_tag="VYN66_000497"
     CDS             9248..10087
                     /locus_tag="VYN66_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1494563.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10080..10874
                     /locus_tag="VYN66_000498"
     CDS             10080..10874
                     /locus_tag="VYN66_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1494564.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10933..11751
                     /gene="mreC"
                     /locus_tag="VYN66_000499"
     CDS             10933..11751
                     /gene="mreC"
                     /locus_tag="VYN66_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV1494565.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11751..12245
                     /gene="mreD"
                     /locus_tag="VYN66_000500"
     CDS             11751..12245
                     /gene="mreD"
                     /locus_tag="VYN66_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV1494566.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            12339..13517
                     /locus_tag="VYN66_000501"
     CDS             12339..13517
                     /locus_tag="VYN66_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV1494567.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            13741..14520
                     /gene="rpsB"
                     /locus_tag="VYN66_000502"
     CDS             13741..14520
                     /gene="rpsB"
                     /locus_tag="VYN66_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV1494568.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            14599..15639
                     /locus_tag="VYN66_000503"
     CDS             14599..15639
                     /locus_tag="VYN66_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV1494569.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            15885..16208
                     /gene="tnpB"
                     /locus_tag="VYN66_000504"
                     /pseudo
     CDS             15885..16208
                     /gene="tnpB"
                     /locus_tag="VYN66_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(16363..16650)
                     /locus_tag="VYN66_000505"
                     /pseudo
     CDS             complement(16363..16650)
                     /locus_tag="VYN66_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            16641..17039
                     /locus_tag="VYN66_000506"
     CDS             16641..17039
                     /locus_tag="VYN66_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1494570.1"
                     /translation="MKAPIPKAPLAHSFGSASIIAHTIHQKFNLKVPNYRQEEDWAKM
                     GLPITRKEISNWHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(17236..18156)
                     /gene="cysK"
                     /locus_tag="VYN66_000507"
     CDS             complement(17236..18156)
                     /gene="cysK"
                     /locus_tag="VYN66_000507"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV1494571.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYNGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(18441..19076)
                     /locus_tag="VYN66_000508"
     CDS             complement(18441..19076)
                     /locus_tag="VYN66_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV1494572.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDNGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            19132..20430
                     /locus_tag="VYN66_000509"
     CDS             19132..20430
                     /locus_tag="VYN66_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1494573.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSNLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            20427..21089
                     /locus_tag="VYN66_000510"
     CDS             20427..21089
                     /locus_tag="VYN66_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV1494574.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREEGASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            21169..21717
                     /gene="raiA"
                     /locus_tag="VYN66_000511"
     CDS             21169..21717
                     /gene="raiA"
                     /locus_tag="VYN66_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV1494575.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            21853..23826
                     /locus_tag="VYN66_000512"
     CDS             21853..23826
                     /locus_tag="VYN66_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV1494576.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            23823..24275
                     /gene="rplI"
                     /locus_tag="VYN66_000513"
     CDS             23823..24275
                     /gene="rplI"
                     /locus_tag="VYN66_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV1494577.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            24319..25671
                     /gene="dnaB"
                     /locus_tag="VYN66_000514"
     CDS             24319..25671
                     /gene="dnaB"
                     /locus_tag="VYN66_000514"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV1494578.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            25673..25939
                     /locus_tag="VYN66_000515"
     CDS             25673..25939
                     /locus_tag="VYN66_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494579.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            26028..27146
                     /gene="cbpD"
                     /locus_tag="VYN66_000516"
     CDS             26028..27146
                     /gene="cbpD"
                     /locus_tag="VYN66_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV1494580.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSGG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNYQNQSGNISSYGSNNSSTV
                     GWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQTGWLKENGLWYYLGSSGAM
                     KTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      27229..27331
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            27402..27692
                     /locus_tag="VYN66_000517"
     CDS             27402..27692
                     /locus_tag="VYN66_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV1494581.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            27655..28413
                     /locus_tag="VYN66_000518"
     CDS             27655..28413
                     /locus_tag="VYN66_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1494582.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            28451..29458
                     /locus_tag="VYN66_000519"
     CDS             28451..29458
                     /locus_tag="VYN66_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1494583.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            29458..30186
                     /locus_tag="VYN66_000520"
     CDS             29458..30186
                     /locus_tag="VYN66_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494584.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            30305..30763
                     /locus_tag="VYN66_000521"
     CDS             30305..30763
                     /locus_tag="VYN66_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV1494585.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            30765..33197
                     /locus_tag="VYN66_000522"
     CDS             30765..33197
                     /locus_tag="VYN66_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1494586.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKSKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            33518..34171
                     /gene="rr06"
                     /locus_tag="VYN66_000523"
     CDS             33518..34171
                     /gene="rr06"
                     /locus_tag="VYN66_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV1494587.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            34168..35508
                     /gene="hk06"
                     /locus_tag="VYN66_000524"
     CDS             34168..35508
                     /gene="hk06"
                     /locus_tag="VYN66_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV1494588.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSRLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            35591..36118
                     /locus_tag="VYN66_000525"
     CDS             35591..36118
                     /locus_tag="VYN66_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV1494589.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            36197..38089
                     /gene="pspC"
                     /locus_tag="VYN66_000526"
     CDS             36197..38089
                     /gene="pspC"
                     /locus_tag="VYN66_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEV1494590.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSEN
                     NPTVTSSGQDISKKYADEVKSHLEKILSEIQTNLDRSKHIKTVNLINKLQDIKRTYLY
                     ELNVLEDKSKAELPSKIKAELDAAFEQFKKDTLPTEPGKKVAEAKKKVEEAEKKAKAQ
                     KEEDYRNYPTITYKTLELEIAESDVKVKEAELELVKKEADESRNEGTINQAKAKVESE
                     QAEATRLKKIKTDREKAEEEAKRRADAKEQDESKRRKSRVKRGDLGEPATPDKKENDA
                     KSSDSSVGEETLPSPSLKPGKKVAEAEKKVEEAEKKAKDQKEEDHRNYPTITYKTLEL
                     EIAESDVEVKKAELELVKEEAKGSRNEEKVKQAKAEVESKKAEATRLEKIKTDRKKAE
                     EEAKRKAAEEDKVKEKPAEQPQPAPAPKPENPAEQPKAEKPADQQAEEDYARRSEEEY
                     NRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQYNGSWYYLNANG
                     DMATGWFQYNGSWYYLNANGDMATGWLQYNGSWYYLNSNGAMVTGWLQYNGSWYYLNA
                     NGSMATDWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDSYRVNA
                     NGEWVN"
BASE COUNT        12027 a         6240 c         8438 g        11550 t
ORIGIN      
        1 aatattcaaa tttttatttt tcaattttgt gtaaattata tttacagtat cctttacaca
       61 aatctgtaaa aagcaaggct gatttatttt gataaattac ggttatttca ttaaaaaaat
      121 gctataattg aaaggactat atcgaaggag aacaaaatga ctaaacccat tattttaaca
      181 ggagaccgtc caacaggaaa attgcatatt ggacattatg ttggaagtct caaaaatcga
      241 gtattattac aggaagagga taagtatgat atgtttgtgt tcttggctga ccaacaagcc
      301 ttgacagatc atgccaaaga tcctcaaacc attgtagagt ctatcggaaa tgtggctttg
      361 gattatcttg cagttggatt ggatccaaat aagtcaacta tttttattca aagccagatt
      421 ccagagttgg ctgagttgtc tatgtattat atgaatctag tttcgttagc acgtttggag
      481 cgaaatccaa cagtcaagac agagatttct cagaaaggat ttggagaaag cattccgaca
      541 ggattcttgg tctatccaat cgctcaagca gctgatatca cagctttcaa ggctaattat
      601 gttcctgttg ggacagatca gaaaccaatg attgagcaaa ctcgtgaaat tgttcgttct
      661 tttaacaatg catataactg tgatgtcttg gtagagccgg aaggtattta tccagaaaat
      721 gagagagcag ggcgtttgcc tggtttagat ggaaatgcta aaatgtctaa atcactaaat
      781 aatggtattt atttagctga tgatgcggat actttgcgta aaaaagtaat gagtatgtat
      841 acagatccag atcatatccg cgttgaggat ccaggtaaga ttgagggaaa tatggttttc
      901 cattatctag atgtttttgg tcgtccagaa gatgctcaag aaattgctga tatgaaagaa
      961 cgttatcaac gaggtggtct tggtgatgtg aagaccaagc gttatctact tgaaatatta
     1021 gaacgtgaac tgggtcctat tcgtgagcgc cgtattgaat ttgctaagga tatgggagaa
     1081 gtttataata tgattcaaaa aggtagtgaa agagcgcgtg aagttgcggg tcaaacccta
     1141 tctgaggtaa aaggggcaat gggacttcat tactttaact aagtttattt tacaagaaac
     1201 tatcttttct atctcgaaga aaagatagtt ttttatttac ccctgtaaca tttgacaaat
     1261 ttttatagaa tggtatgata gatacaatat aaaaagagtc aagctcaaaa agaaagaaaa
     1321 gaggaaactt cgaatgtcta attgggacac taaatttttg aaaaaaggtt ttaccttcga
     1381 tgatgtattg cttattccag ctgaaagtca tgtgttgcct aacgatgcag atttaacaac
     1441 taaattggca gataatttga ctttaaatat cccaattatt accgctgcca tggacacagt
     1501 tacagagagt caaatggcca ttgctattgc tcgtgcaggc ggtctcggag ttatccataa
     1561 aaacatgtca attgctcaac aagcagacga ggttcgtaag gtaaaacgtt ctgaaaatgg
     1621 agttattatt gatccgttct tcttgacgcc tgaacataca attgctgaag cagatgagct
     1681 tatgggtcgt taccgcatca gtggtgttcc agttgttgaa acacttgaaa atcgtaaatt
     1741 ggttggtatt ttgacaaacc gagatcttcg ttttatttca gattataatc aaccaatttc
     1801 aaaccatatg actagtgaaa atcttgttac tgctcctgtg ggtacggatc ttgcaacggc
     1861 tgagagtatt cttcaagagc atcgtattga aaaacttccg ttggtcgatg aagaaggcag
     1921 tctttctggt ttgatcacta tcaaagatat tgaaaaagtt attgagtttc caaatgcggc
     1981 taaagatgag tttggtcgtc ttctagttgc aggtgcagta ggtgttactt cagatacatt
     2041 tgaacgtgca gaggctcttt ttgaggcagg agcggatgcg attgttattg atactgcaca
     2101 tggtcattct gcaggtgtct tgcgtaaaat tgccgagatt cgtgctcatt tcccagatcg
     2161 gactttgatt gctggaaata ttgctactgc tgaaggtgca cgtgcccttt atgaagcggg
     2221 tgtagacgtt gttaaggttg gtattggacc aggttctatc tgtactactc gtgtgattgc
     2281 tggtgttggt gttccgcaag taacagctat ctacgatgct gcagctgttg cgcgcgaata
     2341 tggtaaaacg attattgctg acggtgggat caagtattct ggagatattg taaaagcact
     2401 tgctgcaggt ggaaatgctg ttatgcttgg atctatgttt gctggaactg atgaagctcc
     2461 aggcgaaact gaaatcttcc aaggacgtaa attcaagact taccgtggta tgggatcaat
     2521 tgctgctatg aagaaaggtt caagcgaccg ttatttccaa ggttctgtca atgaagcaaa
     2581 caagcttgtt ccagaaggaa ttgaaggtcg tgttgcttat aaaggagcgg cagctgatat
     2641 tgttttccaa atgattggtg gtattcgctc tggtatgggt tactgtggtg cagctaacct
     2701 taaagaacta cacgataatg ctcaatttat tgaaatgtct ggtgctggtt tgaaagaaag
     2761 ccatcctcat gatgtgcaaa ttactaatga ggcaccaaat tattctatgt aaaaaacaat
     2821 gaaaagaact ccagtgaaaa caggagttct tttacaatgt tgtcaatttc catttacaga
     2881 agctttacca tcctgaatag tgaagatact tagattttct ggcagatttt gaagatggtc
     2941 taagcttgtt gttgtgataa aggtttggat tgattgagaa atcgtttcta ataattttaa
     3001 ctgtctagtg ttgtcaagtt cactcatcac atcgtcaagc agtaatatcg gagattctgt
     3061 ggtaatgctt tccattaatt cgatttctgc taattttatc gagaggacga gactacgatg
     3121 ttgaccttgg cttccgaaac tagcatccat cccatttata taaaaagaaa tgtcatctcg
     3181 atgaggaccg acaccagtat tctttttaaa taaatctctg gatctacttt tttctaaagc
     3241 aattttgaaa gattcggata agttttgttt gtcagttata ttgacagaag attgatagga
     3301 tattgacaac tcttcgatct gattagagag ttcaaaatgt ttcttacgcc caaatgattc
     3361 tagttttttt atgaaatcta agcggtgatt cattacacga catccataat caactagctg
     3421 atcatctaac acagaaagga atgtttcatc tattttttga tctgatttta ggtaagtgtt
     3481 tctttgcttt aggatgtggt tataattggt taagtcagat aagtagattg gcttaatttg
     3541 cccaagttcc atatcaatga attttcgtcg aatcgaaggt gctcctttaa ttagttgtaa
     3601 atcttcagga gcaaataaga caacattcat gtgtcctaca taatctgaaa ggcgtgcctg
     3661 ttttaagtga ttaacttttg ttacacggcc tttttgtgtt agttcgattt ctaggggaat
     3721 ggatccagtt tttttctgaa cgagacctga aagatgaagt tgttcctcat caaaatgaat
     3781 gagattttta tctgttcgag ttcgatgact acgcgttaag gctaaaaaat agatagcctc
     3841 taacatgttt gtttttcctt gtgcattacg tcccaaaaag acattcaatt taggattaaa
     3901 gtctattttc gtttctttgt aattacgaaa agtcttgaga gatagatgtt gtagccacat
     3961 gattatctac cagggaatcg tggagcttgt tggcttttag gtgatgaagc aggtttcgat
     4021 ttgtcttttt taactccctt attcatcttt ttgacaagtt tagcgatccg ttctttttca
     4081 actttatcag cttggtattc atcttgttct tcagaagtag gttgtgtcaa caagatgtca
     4141 atgttcatgt cagggatgtc aactttatcg ccaatacgaa gtttttttcc acgacgactc
     4201 tctaattccc tattaaagta aacagaatgt tcagagagaa atgatttaat agctcctccg
     4261 ctatgtgtaa ttccaagttc tttgagtagt gcttggaggg taataaattc ttcaaataat
     4321 ttgtattcca taattcacct caatctttgg tattatacca tattttccta aaaatagtga
     4381 gagagacagc gagaaatgta agcgattttt atttcaaaag tgttacaggg aacaagaaaa
     4441 tttcaggttt tcgtgatata atagaagtct gtatataagg aggtaaatca tggagttagt
     4501 gcatggaatt tcaacacatt ttatccaatc aaaaaagttt aaaacaaaca aaattaccgt
     4561 gcgttttacc gctccattat cccttgatac gattgcaggt cacatgttga gtgcaagtat
     4621 gctagagact gctaatcaga tgtaccccac ttctcaagat ttgaggagac acttggccag
     4681 tctatacggt acagatatgt caaccaattg tttcagaaga gggcaaagcc acattataga
     4741 attgacattt acctatgttc gtgatgagtt tttaagtagg aaaaacgtgc taacctctca
     4801 gattttggaa cttgtaaaag aaactctttt ttcacccgta gtagttgata atgggtttga
     4861 tccggcctta tttgaaattg agaaaaaaca attgctagca agtttagcag ctgatatgga
     4921 tgattctttt tattttgcac ataaagaatt ggataaattg ttttttcatg atgaacgtct
     4981 tcaattggaa tatagtgatt tacgaaatcg tattttagct gaaactccac aaagttctta
     5041 ttcttgtttc caagaatttt tagccaatga tcgaatagat ttctttttcc taggtgattt
     5101 taatgaggtt gaaattcaaa atgtattaga atcatttggc tttaaaggtc gaaaaggaga
     5161 tgtgaaggtt cagtattgtc aaccttattc taatatcctt caggaaggta tggttcggaa
     5221 aaatgtggga caatccattt tggaattagg ttatcattac tgttctaaat atggtgatga
     5281 gcaacattta cccatgattg taatgaatgg tttacttggt ggatttgctc actctaagct
     5341 ctttacaaat gtccgtgaaa atgctggatt agcttatacc atttcaagtg agcttgattt
     5401 atttagtgga ttcttgagga tgtatgctgg tatcaatcga gaaaatcgta accaggctcg
     5461 taaaatgatg aataatcaac tgcttgattt aaaaaaaggt tattttacag agtttgagtt
     5521 aaatcagacc aaggaaatga ttcgttggtc gttgttactt tctcaagata atcaatcttc
     5581 attgattgaa cgtgcttatc aaaatgcctt atttggaaaa tcttcagcag actttaaaag
     5641 ttggattgca aagcttgaac agattgacaa agatgctatt tgtagagtag ctaataatgt
     5701 gaaactacaa gcgatttact ttatggaagg aatagaatga caaaggttgt ttttgaagaa
     5761 aaatactatc cagctgtaaa agaaaaggtt tatcgaactc gtttggccaa cggattgaca
     5821 gttgctcttt tgcctaaaaa ggaatttaaa gaggtttacg ggagtgtaac tgtacagttt
     5881 ggttcgatag atacgcttgt cacagaagtt gacggagatg taaaacaata tcctggagga
     5941 attgctcatt ttcttgaaca taaattattt gagagagaag attctagtga tttgatgtcg
     6001 gcttttacga gtctaggtgc agatagtaat gcctttacaa gctttacaaa aacaaactat
     6061 cttttttcag caacggatta ttttttagaa aatttatatt tacttgatga attggtaaca
     6121 tcagcacact ttactgaagc ttccattctg acagagcagg atattattca gcaagaacga
     6181 gaaatgtacc aagatgatcc agattcgtgt ttattctttt caactttagc gaatttgtat
     6241 cctggtacac ctttagcaac tgatatagtt ggaagtgagg agtccatttc ccaaatcaat
     6301 ctaactaatt tgcaagaaaa ttttacaaag ttttacaaac ctgtaaacat gtctctgttt
     6361 ttagttggta attttgatgt ggagcgagta caggactatt ttgaaagcaa agaactgaaa
     6421 gattcagatt ttcaggaagt agcaagagaa aagttgtttt tacagcctgt aaagccaaca
     6481 gatagtatga gaatggaagt atcttctccc aaactagcga ttggagttag aggtaagcga
     6541 gaagtttctg aagcggattg ctatcgacat catattttat taaaattatt gtttgcaatg
     6601 atgtttggtt ggacttcgga tcgttttcaa aaatgttatg aatcaggtaa aattgatgcg
     6661 tccttatctc tggaagttga aataacaagt cgctttcatt ttgtcatgtt gacaatggat
     6721 acgaaagagc cagttgcttt gtctcatcaa tttaggaagg ctattcgtaa ttttacaaag
     6781 gatttagata ttacagagga acatttagat attatcaaaa gagagatgtt tggcgaattt
     6841 ttcagtagca tgaactctct tgaatttatt gcaacgcaat atgatgcttt tgaaaatggt
     6901 gagacaattt ttgatttgcc gaaaatttta caggaaatta ctttagagga tgtccttgat
     6961 gctggacatc atttaataga tgatggtgac atagttgatt ttacaatatt cccatcgtag
     7021 taacctatta taatagacac tagaaagaag ggatgacaag tatgagaaaa aaaacaattg
     7081 gagaggtttt acgattagct agaatcaatc agggattgag tttagatgaa ttgcagaaaa
     7141 agacagaaat ccagttagat atgttggaag caatggaagc agacgatttc gatcaacttc
     7201 caagtccttt ttacacgcgt tctttcttga aaaaatatgc atgggctgtt gagttagatg
     7261 accaaattgt tttggatgct tatgattctg ggagtatgat tacttatgag gaagtagatg
     7321 ttgatgaaga tgagttgaca ggtcgtagac gttcaagtaa gaaaaagaag aaaaaaatat
     7381 catttttacc tttattttat tttatctttt ttgctttatc gattttaatt tttgtgactt
     7441 attatgtttg gaactatatt caaactcaac cagaggagcc ttctctttct aattacagtg
     7501 tggttcaatc aacaagttca actagctctg ttccccactc ctcaagtagt agttcttcta
     7561 gtatagaatc agctataagt gtatcaggcg aaggaaatca tgtagaaatc gcttataaga
     7621 caagtaagga aacagttaaa ttgcaattgg cagtttcaga tgttacaagt tgggtcagtg
     7681 tttcagaaag cgaacttgag ggcggtgtaa ccttatcgcc aaagaagaaa agtgcagaag
     7741 caacagttgc aactaaaagt cctgtaacaa ttacgttagg tgttgtaaaa ggtgttgatt
     7801 tgacagtaga taatcagact gttgatttat cgaaattaac agctcagact ggacaaatca
     7861 ctgtaacctt tactaaaaat taaggaaaaa cgaatgaaaa aagaacaaat tcccaatctc
     7921 ttaacaatag gtcgaattct ctttatacct atttttatct ttattttaac gataggaaat
     7981 tcgatagaga gtcatatagt tgcagctatt atctttgctg ttgccagtat taccgactat
     8041 ttagatggat atttagctcg taaatggaat gtggtcagta attttggtaa atttgcagat
     8101 cctatggcgg ataagttact agttatgtcg gcttttatta tgttgattga gttaggtatg
     8161 gctccggctt ggattgttgc agtgattatc tgtcgtgagt tagctgtgac aggtttaagg
     8221 cttttattgg ttgaaactgg tggaacaatt ttagcagcag caatgcctgg aaaaattaaa
     8281 acttttagtc agatgtttgc cattattttc ttgctattac attggacttt gcttggtcaa
     8341 gttctacttt atgtagcctt atttttcact atctactctg gctatgacta tttcaagggt
     8401 agtgcctatg tatttaaagg gacatttggt tcgaaatgaa atcaataatt gatgtaaaaa
     8461 atctttcttt tcgctataaa gaaaatcaga actactacga tgtgaaggat attacgtttc
     8521 acgtgaaacg tggagaatgg ctttcgattg tagggcataa tggtagtggt aaatcaacga
     8581 cggttcgatt aattgatggc ttactggaag cagaatccgg agagattgta attgatggcc
     8641 aacgtttgac tgaggaaaat gtttggaata tacgtcgtca aatcggtatg gtttttcaaa
     8701 atccagacaa tcaatttgtt ggagcgactg ttgaagatga tgttgctttt ggtttggaaa
     8761 atcagggact ttctcgtcaa gaaatgaaaa agagagtgga agaagctctg gctttagttg
     8821 gcatgttgga ctttaaaaag agagagccag cgcgtctatc aggtggccaa aagcaacgtg
     8881 tggccattgc aggtgttgta gccctaagac cagctatttt aatcttagat gaagcaacga
     8941 gtatgttgga tcctgagggg cgtagagaac ttattgggac agtaaaagga attcgaaaag
     9001 actatgatat gacggtcatt tctattaccc atgatttgga agaagtcgcc atgagtgatc
     9061 gcgtattggt catgaaaaaa ggggaaattg aatcaactag tagtccaagg gagcttttct
     9121 ctcgaaatga tttagatcaa attggattag acgatccttt tgctaatcaa ttaaaaaaat
     9181 ctttgagcca gaatggctat gatttacctg aaaattattt gacagaaagt gagctagagg
     9241 ataagctatg ggaattgctc tagaaaatgt gaattttaca tatcaagaag gtactccctt
     9301 agcttcagca gctttgtcgg atgtttcttt gacgattgaa gatggctctt atacagcttt
     9361 aattgggcac acaggtagtg gtaaatcaac tattttacaa ctcttaaatg gtttattggt
     9421 gccaagtcaa gggagtgtga gggtttttga taccttaatc acctcgactt ctaaaaataa
     9481 agatattcgt caaattagaa aacaggttgg cttggtattt cagtttgctg aaaatcagat
     9541 ttttgaagaa acggttttga aggacgttgc ttttggaccg caaaattttg gagtttctga
     9601 agaagatgct gtgaagactg cgcgtgagaa actggctctg gttggaattg atgaatcact
     9661 ttttgatcgt agtccgtttg agctgtcagg gggacaaatg agacgtgttg ccattgcagg
     9721 catacttgcc atggagccag ctatattagt cttagatgag ccaacagctg gtctagatcc
     9781 tctagggaga aaagagttga tgaccctgtt caaaaaactc caccagtcag ggatgaccat
     9841 cgtcttggta acgcatttga tggatgatgt tgctgaatat gcgaatcaag tctatgtaat
     9901 ggaaaaggga cgtttagtaa aggggggcaa accaagtgat gtctttcaag acgttgtttt
     9961 tatggaagaa gttcagttgg gagtacctaa aattacggcc ttttgtaaac gattggctga
    10021 tagaggcgtg tcatttaaac gattaccgat taagatagag gagttcaagg agtcgctaaa
    10081 tggatagtat gattttaggg cgttatatcc caggggattc gattgttcac cgattggatc
    10141 cacgtagcaa attgcttgct atgatgctac tgattttgat tgtgttttgg gctaataacc
    10201 ccttgacgaa tctgattctt tttatagcga cagggatatt tattgccttg tcaggagtat
    10261 ctctttcatt ttttattcag ggcttgaagt ctatgttttt cttgattgcc ttcacaacta
    10321 tttttcaact atttttcatt tctaatggga atgttttatt tgagttttcg tttgtgagaa
    10381 tcacggatta tgctttgcaa caagctggga ttattttttg tcgctttgta ttgattattt
    10441 tcttttcaac tttgttaacc ttaacaacca tgcctttaag tttggcatca gctgtcgaag
    10501 ctttattagc acctttaaag cgtgtgaaag ttccagttca tgaaattgga ttgatgctgt
    10561 ctatgagttt gcgttttgtc ccaaccttga tggatgatac gactcggatt atgaatgcac
    10621 agaaagctcg tggagtggat tttggagaag gaagcatcgt tcaaaaagta aaggcgatga
    10681 ttcccatttt gattcctctt tttgcgacaa gtttaaaacg tgcagattcc ttggctatcg
    10741 ctatggaagc gcgtggctat cagggtggaa aaggcagaag tcaataccga caattgaaat
    10801 ggactctgaa ggatacgctg accattcttg ttattctcgt acttggttgt tgtttatttt
    10861 tcttaaaatc ttagtagctt tcaggaattg ataaaaagtt actgtaacag tttttgatat
    10921 aaaggtagga atatgaaccg ttttaaaaaa tcaaaatatg tcattattgt ttttgtcact
    10981 gttctgcttg tgtcagctct cttagcgacg acttattcaa gtacaattgt gacaaaatta
    11041 ggagatggaa tctcattggt tgatagagtt gtacaaaaac cttttcagtg gtttgattct
    11101 gtcaaatcag atttggctca tttgacacga acatataatg aaaatgaaag tttgaagaaa
    11161 cagctttacc aattagaagt taaatcaaat gaggtggaaa gtttaaagac agaaaatgaa
    11221 caactgcgcc aattgcttga tatgaagtct aaattgcaag ccacaaagac tttagcagca
    11281 gatgttatta tgcgttctcc ggtatcttgg aagcaggagt tgaccttaga tgcaggtaga
    11341 tcaaaaggtg cttctgagaa catgttagct attgcaaatg gtggcttgat tgggagtgtt
    11401 tcaaaagtag aggagaactc tactatagtc aaccttctga caaatacgga aaatgctgat
    11461 aagatttctg ttaaaattca acatggctct actacaattt atggaattat tattggctat
    11521 gacaaggaaa atgacgttct taaaattagc caattaaata gtaatagcga tattagtgcg
    11581 ggagataagg tgactactgg tggattagga aactttaacg ttgctgatat tcctgttggt
    11641 gaagtggttg ccacaacgca tagtacagac tatttgacac gagaagtaac tgttaaattg
    11701 agtgcagata ctcataatgt agatgtgata gaattagtgg ggaattcata atgagacagt
    11761 tgaagcgagt tggagtattt ttattgcttc ctttctttgt tctaattgac gcccatatta
    11821 gccagcttct gggctcattt ttcccccatg tacatttggc tagtcatttt ctttttctat
    11881 ttctcttatt tgagacgata gaagtatcag agtatctcta cctagtctat tgttttgtta
    11941 taggcttggt ttatgatgtt tactttttcc atctaatagg gattacaact ctcttattta
    12001 tcttattggg agccttcctt cataaattga atagtgttat tttgttgaat cgttggacaa
    12061 gaatgctagc tatgattgtg ctgacattcc tgtttgaaat gggtagttat cttttggctt
    12121 ttatggtagg gttgacagta gatagcatgt cgatttttat agtctatagc ttggtaccga
    12181 cgatgatttt aaatttttta tggattactg tttttcaatt tatttttgaa aaatattatc
    12241 tataagaacg acatataaat gtaacaaagg cgtaatattt attaggcctt tttttggtat
    12301 actagtattg tctttaaaag aaggagtatc tacgtaatat gaagaaaaaa atcttagcgt
    12361 cacttttatt aagtacagta atggtttctc aagtagctgt tttaacaact gcgcatgcag
    12421 aaacgactga tgacaaaatt gctgctcaag ataataaaat tagtaactta acagcacaac
    12481 aacaagaagc ccaaaaacaa gttgaccaaa ttcaggagca agtatcagct attcaagctg
    12541 agcagtctaa cttgcaagct gaaaatgata gattacaagc agaatctaag aaactcgagg
    12601 gtgagattac agaactttct aaaaacattg tttctcgtaa ccaatcgttg gaaaaacaag
    12661 ctcgtagtgc tcaaacaaat ggagccgtaa ctagctatat caataccatt gtaaactcaa
    12721 aatcaattac agaagctatt tcacgtgttg ctgcaatgag tgaaatcgta tctgcaaaca
    12781 acaaaatgtt agaacaacaa aaggcagata aaaaagctat ttctgaaaaa caagtagcaa
    12841 ataatgatgc tatcaatact gtaattgcta atcaacaaaa attggctgat gatgctcaag
    12901 cattgactac gaaacaggca gaactaaaag ctgctgaatt aagtcttgct gctgagaaag
    12961 cgacagctga aggggaaaaa gcaagtctat tagagcaaaa agcagcagct gaggcagagg
    13021 ctcgtgcagc tgcggtagca gaagcagctt ataaagaaaa acgagctagc caacaacaat
    13081 cagtacttgc ttcagcaaac actaacttaa cagctcaagt gcaagcagta tctgaatctg
    13141 cagcagcacc tgtccgtgca aaagttcgtc caacatacag tacaaacgct tcaagttatc
    13201 caattggaga atgtacatgg ggagtaaaaa cattggcacc ttgggctgga gactactggg
    13261 gtaatggagc acagtgggct acaagtgcag cagcagcagg tttccgtaca ggttcaacac
    13321 ctcaagttgg agcaattgca tgttggaatg atggtggata tggtcacgta gcggttgtta
    13381 cagctgttga atcaacaaca cgtatccaag tatcagaatc aaattatgca ggtaatcgta
    13441 caattggaaa tcaccgtgga tggttcaatc caacaacaac ttctgaaggt tttgttacat
    13501 atatttatgc agattaattt acagagggac tcgaatagag ccctcttttc aggttttacc
    13561 gtgacaatcc ctattaaaaa ttatatcaaa atagcttgaa aatattggaa aagtatggta
    13621 gaatgaaaat tgtcgtgtga acgataatac tcattcttga tgaattgtga agcagttgcc
    13681 cttgggtcgt tttgcgagtt gaagtcaaga agaggaaaaa aacaaaaagg agaaatactc
    13741 atggcagtaa tttcaatgaa acaacttctt gaggctggtg tacactttgg tcaccaaact
    13801 cgtcgctgga atcctaagat ggctaagtac atctttactg aacgtaacgg aatccacgtt
    13861 atcgacttgc aacaaactgt aaaatacgct gaccaagcat acgacttcat gcgtgatgca
    13921 gcagctaacg atgcagttgt attgttcgtt ggtactaaga aacaagcagc tgatgcagtt
    13981 gctgaagaag cagtacgttc aggtcaatac ttcatcaacc accgttggtt gggtggaact
    14041 cttacaaact ggggaacaat ccaaaaacgt atcgctcgtt tgaaagaaat taaacgtatg
    14101 gaagaagatg gaactttcga agttcttcct aagaaagaag ttgcacttct taacaaacaa
    14161 cgtgcgcgtc ttgaaaaatt cttgggcggt atcgaagata tgcctcgtat cccagatgtg
    14221 atgtacgtag ttgacccaca taaagagcaa atcgctgtta aagaagctaa aaaattggga
    14281 atcccagttg tagcgatggt tgacaccaat actgatccag atgatatcga tgtaatcatc
    14341 ccagctaacg atgacgctat ccgtgctgtt aaattgatca cagctaaatt ggctgacgct
    14401 attatcgaag gacgtcaagg tgaggatgca gtagcagttg aagcagaatt tgcagcttca
    14461 gaaactcaag cagattcaat tgaagaaatc gttgaagttg tagaaggcga caacgcttaa
    14521 tttatacaaa tagtaattac ctaggagggc ggggcttagc ccggctctcc tattttcaaa
    14581 aaatatagga gaattaaaat ggcagaaatt acagctaaac ttgtaaaaga gttgcgtgaa
    14641 aaatctggtg ccggtgttat ggacgctaaa aaagcgcttg tagaaacaga cggtgacatc
    14701 gaaaaagcga ttgaattgct tcgtgaaaaa ggtatggcta aggcagctaa gaaagctgac
    14761 cgtgttgctg cagaaggttt gactggtgtt tatgttaacg gtaatgttgc agcagttatt
    14821 gaagtaaacg ctgaaactga cttcgttgca aaaaacgctc aattcgttga attggtaaat
    14881 actacagcta aagtcattgc tgaaggaaaa cctgctaaca acgaagaagc tcttgctttg
    14941 ataatgcctt caggtgaaac tcttgaagct gcatacgtat ctgcaacagc aactatcgga
    15001 gagaaaatct cattccgtcg ctttgcattg attgaaaaaa cagacgcaca acactttgga
    15061 gcataccaac ataacggtgg acgtatcggt gttatttcag ttgttgaagg tggagacgaa
    15121 gcacttgcta aacaattgtc aatgcacatc gcagcgatga aaccaacagt tctttcttac
    15181 aaagaattgg atgagcaatt cgttaaagat gagttggcac aattgaatca cgttatcgac
    15241 caagacaacg aaagccgtgc aatggttaat aaaccagctc ttccacactt gaagtatgga
    15301 tcaaaagctc aattaactga tgatgttatt gctcaagctg aagctgacat caaagctgaa
    15361 ttggctgcag aaggtaaacc agaaaaaatc tgggacaaaa ttattccagg taaaatggat
    15421 cgcttcatgc ttgataacac taaagttgac caagcttaca cacttcttgc acaagtttac
    15481 atcatggatg acagcaagac agttgaagca taccttgaat cagttaacgc ttcggtagtt
    15541 gagtttgctc gctttgaagt tggtgaaggt atcgagaaag ctgcaaacga cttcgaagct
    15601 gaagttgcag ctacaatggc agcagccttg aataactaat aatagaaagg aagcaaattg
    15661 cttccttttt tgtatagggc ttagattttt cctgaaattg ttttttcccc agcctcactc
    15721 gattagaata attaaaacaa aaagccctat aataagggct aagtgcattt aggagactat
    15781 acttcaaatt catagtaagc gcgtcataac aaggtatcta tcctaattcc ccaacttcca
    15841 gtatactaga taaaaaataa ttatcggagg aaaggtattg tactatgata attcaactaa
    15901 gtgatttagg tcaagttcac cttgtttgtg acaagacaga tatgaggtag ggaatagact
    15961 cattagccta tgtagttaaa acccactttg aattggattc tttctccggt caagtctttc
    16021 tcttttgtgg tgaacgtaaa gaccgcttta aagcccttta ctgggatggt caaggatttt
    16081 ggctactata taaacgcttt gagaacggaa aactgacttg gctaagtaca gaaaaggatg
    16141 tcaaagctct cgcacctgaa caagtagatt ggcttatgaa gggcttttct atcactccaa
    16201 aaatatagta gattgaaact agaatagtac acctctgctt ctaaaacatt gttagaaatc
    16261 gatttgattg tcctgatcga tttgtcctgt tcttatttca ttttactata aatccatcag
    16321 aaagttgtga tttctattga aatgaggact ttctttttac actcatctgc tttcaaaaag
    16381 cattctagtc catctccgat taacgatgga ctttatcacc tccttctcca gtccttgtat
    16441 gacatcttcc aaagttcgaa aggctttatt cttaaatcca cgtttacgaa tctctttcca
    16501 tacttgttca atggggttca tctctggtgt gtatggagga ataaatgcaa agccaatatt
    16561 agtcggaatc tttaaggtac ttgatttatg ccatatagca ttgtccataa cgagtaaaag
    16621 ataatcatct ggataagctt gtgaaagctc ctattcctaa agccccttta gcgcatagct
    16681 ttggctcagc ttctattatc gctcacacca tccatcagaa gtttaatctg aaggtaccca
    16741 attatcgcca agaagaagat tgggctaaga tgggtttacc aatcacacgc aaggaaatct
    16801 ctaattggca tatcaagacg agtcaatact atttggagcc cctctataac ctcttgcgag
    16861 agagactatt gactcagccc ttacttcatg cggatgaaac ttcttatagg gtgctagaga
    16921 gtgatagtca gctgacttac tattggactt ttttgtctgg gaaagctgag aaacaaggga
    16981 ttacgcttta ccatcatgtc ctaatcgatt tgttcatatc ttatttcaat ccattataaa
    17041 tagtgagaaa tatctatcct atcttctaga atgtcttcca aacgtggaaa ctctcgtaaa
    17101 caaagaggtt ttagaggctt atttaccatg gactaaaatt gtacaagaaa agtgcaaata
    17161 agaaatctcc agattaggaa ctatccgtga gttctctagt ctggagattt ttcaatatac
    17221 ttcgttattg gacggttaca attcataaag tgctgtagag agataacgtt caccgttatc
    17281 tggtgctagg gcaaggactt ttttacctgt acctaatttt ttggcaacct cgatggctcc
    17341 gtagatagct gcagctgagg aaatccctac aaggaagcct tcttttccac caatttcacg
    17401 tccgagtgca agagcgtcat ctgatgttac acgaacgata ccattatagg ctttagtatc
    17461 aagtgtatca ggaataaatc cagctgagat accttgaatt ttgtgaggac caggtttttc
    17521 accagataga atagcagatt catctgcttc tactgcaaaa acttgaatgt tagaattttc
    17581 tgatttgagt gcatgagaaa caccagaaat cgttccaccg gtacctactc cagcaacaaa
    17641 ggcatctaat ccatctttac cgaaagcagc tagtatctca gctcctgttg ttctttcgtg
    17701 tacttctgga ttagctggat tgtcaaattg aagaggaagg aaaccatcac gttcagcagc
    17761 gatttcttga gccttagcaa tagcaccttt cattccctcg ctaccaggag ttaggacgag
    17821 ttcagcacca taagcttgga taattttacg tcgttctaca ctcatagttt caggcataac
    17881 gatgacgact ttataccctt tagcagcacc tacccatgaa agtccaatac cagtgtttcc
    17941 acttgttgct tcaacaatag tagaaccagg tttcagaata ccatcttgtt cagctttttc
    18001 aatcatgcta agggcaatac ggtcttttac agatgaacca ggattaaatg cttcaagctt
    18061 tatatagacg tctgcagcac cttctggcac gatgttgtta agtttaacaa tcggtgtttg
    18121 accgattaat tcagtaatgt tgttataaat agacatatga atacctcttt gtataagata
    18181 tctttagtat aacgctatcc ataccatttg taaaatatag aaatcctatc gaagcgatag
    18241 gatttctata tatcatagat caaagtcatt aattttcaag tgattgcgtt cagctaattc
    18301 tagtaaatag gtgtgaagga aaacgctatc aggtttcgtt tgaattgctt cattttctag
    18361 tatattttgg tacgcatctt tttattttgc tcgctaaagt agttgtgaga atgtcaacga
    18421 aacgctgtat tattcattgt ttacactaag tttacaggaa cttcaacctc tcgtaaacct
    18481 tggtcagtta aagtgacttt tccattaaaa aactccacaa gtgcagcttt aatagtttct
    18541 ttttcttctt tatcaacata aatcatcgta tcgacttgat ctgtaaagtt tgtatccagc
    18601 tccatgagat catgttcttt aaggaagtta ctgtactctt ggtactgagc ataagacatt
    18661 tgaatagcaa tgccagcctg ttcttttatt tcaataatac caatttcttt gacagctaag
    18721 gcgacactgc cggcgtaagc acgaattagt cctccagcgc ctagtttaat accaccaaag
    18781 tagcgtgtca cgaccacaca gacattggtg agattgtgat tttctagtac cccaagcatg
    18841 ggaacaccag cagtaccact aggctcacca ttatcacttg tacgtttaat ttcactacgt
    18901 tctccaataa taaaggcaga gcagttatgt gtcgctttgt agtgttcttt tttgatggca
    18961 gtaatgaagt cacgagcctc ttcttcgcta taaacacgct tggcatggca gataaagcga
    19021 gattttttta tttcttcttg gacttggccg tcctctttaa ttgtcctaaa ttccatgatt
    19081 taattgtact aaaaaataat ctaaagtgct agtttttacg aatagagaag tatgaaagta
    19141 aatttagatt atctcggtcg tttatttact gagaatgaat taacagaaga agaacgtcag
    19201 ttggcggaga aacttccagc aatgagaaag gagaagggga aacttttctg tcaacgttgt
    19261 aatagtacta ttctagaaga atggtatttg cccatcggtg cttactattg tcgagagtgc
    19321 ttgctgatga agcgagtcag aagtgatcaa actttatact attttccgca ggaggatttt
    19381 ccgaagcaag atgttctcaa atggcgcggc caattaactc cttttcaaga gaaggtgtca
    19441 gagggactgc ttcaagcagt agacaagcaa aagccaacct tagttcatgc ggtaacagga
    19501 gctggaaaga cagaaatgat ttatcaagta gtggctaaag tgatcaatgc gggtggtgca
    19561 gtgtgtttgg ctagtcctcg catagatgtt tgtttggagc tgtacaagcg cctgcaacag
    19621 gatttttctt gcgggatagc tttgctacat ggagaatcgg aaccttattt tcgaacacca
    19681 ctagttgttg caacaaccca tcagttattg aagttttatc aagcttttga tttgctgata
    19741 gtggatgaag tagatgcttt tccttatgtt gataatccca cgctttacca cgctgtcaag
    19801 aatagtgtaa aggagaatgg attgagaatc tttttaacag cgacttcgac caatgagtta
    19861 gataaaaagg tccgtttagg agaactaaaa agactgagtt taccgagacg gtttcatgga
    19921 aatccgttga ttattccaaa accaatttgg ttatcggatt ttaatcgcta cttagacaag
    19981 aatcgtttgt caccaaagtt aaagtcctat attgagaagc agagaaagac agcttatccg
    20041 ttactcattt ttgcttcaga aattaagaaa ggggagcagt tagaagaaat cttacaggag
    20101 caatttccaa atgagaaaat tggctttgta tcttctgtaa cagaggatcg attagagcaa
    20161 gtacaagctt ttcgagatgg agaactgaca atacttatca gtacgacaat cttggagcgt
    20221 ggagttacct tcccttgtgt ggatgttttc gtagtagagg ccaatcatcg tttgtttacc
    20281 aagtctaatt tgattcagat tggtggacga gttggacgaa gcatggatag accgacagga
    20341 gatttgcttt tcttccatga tgggttaaat gcttcaatca agaaggcgat taaggaaatt
    20401 cagatgatga ataaggaggc tggtctatga agtgcttgtt atgtgggcag actatgaaga
    20461 ctgttttaac ttttagtagt ctcttacttc tgaggaatga tgactcttgt ctttgttcag
    20521 actgtgattc tacttttgaa agaattgggg aagagaactg tccaaattgt atgaaaacag
    20581 agttgtcaac aaagtgtcaa gattgtcaac tttggtgtaa agaaggagtt gaagtcagtc
    20641 atagagcgat ttttacttac aatcaagcta tgaaggattt tttcagtcgg tataagtttg
    20701 atggagactt cctgttaaga aaagttttcg cttcattttt aagtgaggag ttgaaaaagt
    20761 acaaagagta tcaatttgtt gtaattcccc taagtcctga tagatatgct aatagaggat
    20821 ttaatcaggt tgagggcttg gtagaggcag caggctttga gtatctggat ttattagaga
    20881 aaagagaaga gggagccagt tcttctaaaa atcgttcaga gcgcttgggg acagaacttc
    20941 ctttctttat taaaagtgga gtcactattc ctaaaaaaat cctacttata gatgatatct
    21001 atactacagg agcaactata aatcgtgtta agaaactgtt ggaagaagct ggtgctaagg
    21061 atgtaaaaac attttccctt gtaagatgag gaaaaaattt gcaaaaatag aatgaaagcg
    21121 ttataataaa atcataaaaa caaaaatgtt tagaaagaag gtactcatat gattaaatat
    21181 agtatccgtg gtgaaaacct agaagtaaca gaagcaattc gtgattatgt agtttctaaa
    21241 ctcgaaaaga tcgaaaagta cttccaacca gaacaagagt tggatgcccg aattaactta
    21301 aaagtttatc gtgaaaaaac ggctaaagtg gaagtaacga ttccgcttgg atctattact
    21361 ctccgcgcag aagatgtatc tcaagatatg tatggttcaa ttgaccttgt aactgataaa
    21421 attgaacgtc agattcgtaa aaataaaaca aaaatcgagc gtaaaaataa aaataaggta
    21481 gcaactggtc aattatttac agatgctttg gtggaagatt caaatgttgt ccagtctaaa
    21541 gttgttcgtt caaaacaaat tgatttaaaa ccaatggatt tggaagaagc aattctacaa
    21601 atggatttat tggggcatga tttctttatc tatgtggatg ttgaagatca aacaaccaat
    21661 gtgatttatc gtcgtgagga tggcgaaatt ggtttgttag aggttaaaga atcttaattt
    21721 caatatgtgt aaaggtaggt ttactgaatt gtaaactacc tttttcttat aattgataga
    21781 attactgatt acacttttaa aaagtcgctt tttgaggggt attatggtat aatgggtgcc
    21841 aagaggtttt gaatgaaaaa attttatgta agtccaattt ttcctattct agtaggattg
    21901 attgcgtttg gagtcttatc cactttcatt atttttgtta ataataatct gttgacggtt
    21961 ttaattttgt ttctttttgt aggaggctat gtttttttat ttaagaaact gagagtgcat
    22021 tatacaagga gtgatgtaga acagatacag tatgtaaacc accaagcgga agaaagtttg
    22081 acagctctat tggaacagat gcctgtaggt gttatgaaat tgaatttatc ttctggagag
    22141 gttgagtggt ttaatcccta tgctgaattg attttgacca aggaagatgg tgattttgat
    22201 ttagaagctg ttcaaacgat tatcaaggct tcagtaggaa atccgtctac ttatgccaag
    22261 cttggtgaga agcgttatgc tgttcatatg gatgcttctt ccggtgtttt gtattttgta
    22321 gatgtatcca gggaacaagc cataacagat gaattggtaa caagtcgacc agtgattggg
    22381 attgtctctg tggataatta tgatgatttg gaggatgcaa cttctgagtc agatattagt
    22441 caaatcaata gttttgtagc taattttata tcagagtttt cagaaaaata catgatgttt
    22501 tctcgtcggg taagtatgga tcgattttat ctatttactg actacacggt gcttgagggc
    22561 ttgatgaatg ataaattttc tgttattgat gctttcagag aagagtcgaa acagagacag
    22621 ttgcccttga ccttaagtat gggattttct tatggcgatg gaaatcatga tgagataggg
    22681 aaagttgctt tgctcaattt gaacttggct gaagtacgtg gtggcgacca ggtggttgtt
    22741 aaggaaaacg acgaaacgaa aaatccagtt tattttggtg gtgggtctgc tgcttcaatc
    22801 aagcgtacac ggactcgtac gcgcgctatg atgacagcta tttcagataa gattcggagt
    22861 gtagatcagg tttttgtagt cggtcacaaa aatttagaca tggatgcttt gggctctgct
    22921 gtaggtatgc agttgttcgc cagcaatgtg attgaaaata gctatgctct ttatgatgaa
    22981 gaacaaatgt ctccagatat tgaacgagct gtttcattca tagaaaaaga aggagttacg
    23041 aagttgttgt ctgttaagga tgcaatgggg atggtgacca atcgttcttt gttgattctt
    23101 gtagaccatt caaagacagc cttaacatta tcaaaagaat tttatgattt atttacccaa
    23161 accattgtta ttgaccacca tagaagggat caggattttc cagataatgc ggtcattact
    23221 tatatcgaaa gtggtgcaag tagtgccagt gagttggtaa cggaattgat tcagttccag
    23281 aattctaaga aaaatcgttt gagtcgtatg caagcaagtg ttttgatggc tggtatgatg
    23341 ttggatacta aaaatttcac ctcgcgagta actagtcgga catttgatgt tgctagctat
    23401 ctcagaacgc gcggaagtga tagtattgct atccaggaaa tcgctgcgac agattttgaa
    23461 gaatatcgtg aggtcaatga acttatttta caggggcgta aattaggttc agatgtacta
    23521 atagcagagg ctaaggacac gaaatgctat gatacagttg ttattagtaa ggcagcagat
    23581 gccatgttag ccatgtcagg tattgaagcg agttttgttc ttgcgaagaa tacacaagga
    23641 tttatctcta tctcagctcg aagtcgtagt aaactgaatg tacaacggat tatggaagag
    23701 ttaggcggtg gaggccactt taatttggca gcagctcaaa ttaaagatgt aaccttgtca
    23761 gaagcaggtg aaaaactgac agaaattgta ttaaatgaaa taaaggaaaa ggagaaagaa
    23821 gaatgaaagt aatcttttta gcagatgtta aaggaaaagg taaaaaaggc gaaattaagg
    23881 aagtaccaac agggtatgcg caaaactttc ttatcaaaaa gaatctagcc aaagaagcga
    23941 ctgctcaagc tgtaggtgaa cttcgtggta aacaaaaatc ggaagaaaaa gctcacgctg
    24001 agatgattgc agaaggaaaa gcaattaaag cacaacttga agcagaagaa actgttgtag
    24061 aatttgttga aaaagttggt ccagatggtc gtacctttgg ttctattacc aataagaaga
    24121 ttgcagaaga attgcaaaag caatttggaa ttaagattga taaacgtcat attcaagtac
    24181 aagctccgat tcgagcggtt ggtttgattg atgtgccagt gaaaatctat caagatatca
    24241 caagtgtaat caatcttcgt gtgaaagaag gataagttta caccttcttg acaagattgt
    24301 aaaaggaagg gaagtctgat ggcagaagta gaagagttac gagtacaacc tcaagatatc
    24361 ttagctgagc aatccgtttt aggggctatc tttattgatg agagtaaact tgtttttgtg
    24421 cgagaataca ttgagtctcg ggactttttt aagtatgccc atcgtttgat tttccaagcc
    24481 atggtcgatt tatccgatcg tggtgatgcc atagatgcaa caacggttcg tactatcctt
    24541 gataatcaag gtgatttaca gaatattggt ggcttgtctt acttggttga gattgttaat
    24601 tctgtgccaa cttctgctaa tgcggagtat tatgctaaga ttgttgcaga aaaagcaatg
    24661 ctacgtcgtt taattgccaa gttgacagag tctgtcaacc aagcttacga agcgtcacaa
    24721 ccagctgatg aaattattgc tcaggcagaa aaagggctga ttgatgtcag tgaaaatgca
    24781 aatcgaagcg ggtttaagaa cattcgagat gtgttgaatc tcaactttgg aaatctggaa
    24841 gctcgctcgc aacaaacgac cgatattaca ggtattgcga caggttatcg tgatttggat
    24901 catatgacaa caggacttca tgaggaggag ttgattatct tagcagctcg tccagcagtt
    24961 ggtaagacag catttgcctt gaatatcgct cagaacattg ggactaagtt ggacaaaacg
    25021 gttgctattt tttcactcga aatgggtgcg gaaagcttgg tagatcgtat gttagctgca
    25081 gaaggcttgg tggagtcaca ttctatccgt acagggcaat tgacagatga ggagtggcaa
    25141 aaatatacta ttgctcaggg taatctagct aacgccagta tctatatcga tgatacgcca
    25201 ggtattcgga ttacagagat tcgttctcgt tctcgtaaat tggctcaaga aactggaaat
    25261 cttggtttga ttttgataga ctatttacaa cttatcacgg gaactggtcg agaaaatcgt
    25321 caacaagaag tttctgaaat ttctcgtcag ttgaaaatac tagccaagga attgaaggtt
    25381 ccagtaatcg ctctgagtca gctttctcgt ggtgtagaac aacgtcagga caagagaccg
    25441 gtcttgtctg atattcgtga atctgggtct attgagcagg acgctgatat cgtagctttt
    25501 ctctatcgcg atgactacta tgaacgtggt ggtgaagaag aggagggtat cccaaataat
    25561 aaggtggaag ttattatcga gaaaaaccgt agtggagctc gtggaacagt ggaattgatt
    25621 ttccaaaaag aatacaataa attttcaagt atctcaaaga gggaggcata agatgtcaga
    25681 tgcatttaca gatgtagcca agatgaaaaa aatcaaagaa gaaatcaagg cacatgaggg
    25741 acaagtcgta gaaatgactt tggagaatgg tcgtaagcgc caaaaaaata gattgggtaa
    25801 gctaattgaa gtttatccat ccctatttat tgtggagttt ggggatgtgg aaggagataa
    25861 acaagttaat gtttacgttg aatcctttac ttactcagat attcttacag aaaagaattt
    25921 gattcattat cttgactaaa gtgagaaatt ttctcacttt ttcttttttc tccgaataat
    25981 ttaggtgaag gcaatcatcg ctttatatta tttttcaagg aggaagaatg aaaattttac
    26041 cgtttatagc aagaggaaca agttattact tgaagatgtc agttaaaaag cttgttcctt
    26101 ttttagtagt aggattgatg ctagcagctg gtgatagtgt ctatgcctat tccggaggaa
    26161 atggatcgat tgcgcgtggg gatgattatc ctgcttatta taaaaatggg agccaggaga
    26221 ttgatcagtg gcgcatgtat tctcgtcagt gtacttcttt tgtagccttt cgtttgagta
    26281 atgtcaatgg ttttgaaatt ccggcagctt atggaaatgc gaatgaatgg ggacatcgtg
    26341 ctcgtcggga aggttatcgt gtagataata caccgacgat tggttccatt acttggtcta
    26401 ctgcaggaac ttatggtcat gttgcctggg tgtcaaatgt aatgggagat cagattgaga
    26461 ttgaggaata taactatggt tatacagaat cctataataa acgaattata aaagcaaaca
    26521 cgatgacagg atttattcat tttaaagatt tggatggtgg cagtgttggg aatagtcaat
    26581 cctcaacttc aacaggcgga actcattatt ttaagaccaa gtctgctatt aaaactgaac
    26641 ccctagttag tgcaactgtg attgattact attatcctgg agagaaggtt cattatgatc
    26701 aaattctcga aaaagacggc tacaagtggt tgagttatac ggcttataac ggaagtcgtc
    26761 gctatataca gctagaggga gtgacttctt cacaaaatta tcagaatcaa tcaggaaata
    26821 tctctagcta tggatccaat aatagttcaa ctgtcggttg gaagaaaata aatggtagtt
    26881 ggtatcattt caaatcaaat ggttctaaat caacaggatg gctgaaagac ggttctagct
    26941 ggtattattt gaaattatct ggtgaaatgc agacaggatg gttaaaggaa aatggtttgt
    27001 ggtattatct gggtagttca ggggcaatga aaacaggctg gtaccaggtc tctggtaagt
    27061 ggtattattc ttactcttca ggcgccttag ctgttaatac gacggtggat ggctacagag
    27121 taaacagtga tggagaacga gtatagaaaa ttggagtagg agaaatttcc tgctcttttc
    27181 tgtaagcagt ttccttgaca tttttcttat tttgcgctat catatataca tataatatat
    27241 gggagtctgt gtacagtctg agaggaagtg ttaaacttcg accgcacctg atctgggtaa
    27301 tgccagcgga gggaacgata cttagtctaa ttttgcacct tttccatgta tggtaaaggt
    27361 ttttcttttt tttaaaagga aaacgagaag aggaggttct tatgaaagca agcattgcct
    27421 tgcaagtttt acccctagca caggggattg atcggatagc tgttattgat caggtcattg
    27481 cttatctgca aactcaagaa gtgacgatgg tagtgacacc atttgaaacg gtcttggaag
    27541 gggagtttga tgagcttatg cgcattctaa aagaagcgct ggaagtggca gggcaggagg
    27601 cagacaatgt ctttgccaat gtcaaaataa atgtaggaga gattttaagt attgatgaga
    27661 aacttgaaaa gtatactgag acgacacatt agtctattgg gctttctcgg agtattgtca
    27721 atctggcagt tagcaggttt tcttaaactt ctccccaagt ttatcctgcc gacacctctt
    27781 gaaattctcc agccctttgt tcgtgacaga gaatttctct ggcaccatag ctgggcgacc
    27841 ttgagagtgg ctttactggg gctgattttg ggagttttga ttgcctgtct tatggctgtg
    27901 ctcatggata gtttgacttg gctcaatgac ctgatttacc ctatgatggt ggtcattcag
    27961 accattccga ccattgccat agctcctatc ctggttttgt ggctgggtta tgggattttg
    28021 cccaagattg tcttgattat cttaacaaca acctttccca tcatcgttag tattttggac
    28081 ggttttaggc attgcgacaa ggatatgctg actttgttta gtctgatgcg agcaaaccct
    28141 tggcaaatcc tgtggcattt taaaatcccg gttagcctgc cttactttta tgcaggtctg
    28201 agggtcagtg tctcctacgc ctttatcaca actgtggtat ctgagtggtt gggaggtttt
    28261 gaaggtcttg gtgtttatat gattcagtct aaaaaactgt ttcagtatga taccatgttt
    28321 gccattatta ttctggtgtc gattatcagt cttttgggta tgaagctggt cgatatcagt
    28381 gaaaaatatg tgattaaatg gaaacgtact taaaatagag cgtttttgaa aaagaaaaga
    28441 ggaaatcaaa atgaagaaaa catggaaagt gtttttaacg cttgtaacag ctcttgtagc
    28501 tgttgtgctt gtggcctgtg gtcaaggaac tgcttctaaa gacaacaaag aggcagaact
    28561 taagaaggtt gactttatcc tagactggac accaaatacc aaccacacag ggctttatgt
    28621 tgccaaggaa aaaggttatt tcaaagaagc tggagtggat gttgatttga aattgccacc
    28681 agaagaaagt tcttctgact tggttatcaa cggaaaggca ccatttgcag tgtatttcca
    28741 agactacatg gctaagaaat tggaaaaagg agcaggaatc actgccgttg cagctattgt
    28801 tgaacacaat acatcaggaa tcatctctcg taaatctgat aatgtaagca gtccaaaaga
    28861 cttggttggt aagaaatatg ggacatggaa tgacccaact gaacttgcta tgttgaaaac
    28921 cttggtagaa tctcaaggtg gagactttga gaaggttgaa aaagtaccaa ataacgactc
    28981 aaactcaatc acaccgattg ccaatggcgt ctttgatact gcttggattt actacggttg
    29041 ggatggtatc cttgctaaat ctcaaggtgt agatgctaac ttcatgtact tgaaagacta
    29101 tgtcaaggag tttgactact attcaccagt tatcatcgca aacaacgact atctgaaaga
    29161 taacaaagaa gaagctcgca aagtcatcca agccatcaaa aaaggctacc aatatgccat
    29221 ggaacatcca gaagaagctg cagatattct catcaagaat gcacctgaac tcaaggaaaa
    29281 acgtgacttt gtcatcgaat ctcaaaaata cttgtcaaaa gaatacgcaa gcgacaagga
    29341 aaaatggggt caatttgacg cagctcgctg gaatgctttc tacaaatggg ataaagaaaa
    29401 tggtatcctt aaagaagact tgacagacaa aggcttcacc aacgaatttg tgaaataatg
    29461 acagaaatta gactagagca cgtcagttat gcctatggtc aggagaggat tttagaggat
    29521 atcaacctac aggtgacttc aggcgaagtg gtttccatcc taggcccaag tggtgttgga
    29581 aagaccaccc tctttaatct aatcgctggg attttagaag ttcagtcagg gagaattgtc
    29641 cttgatggtg aagaaaatcc caaggggcgc gtgagttata tgttgcaaaa ggatctgctc
    29701 ttggagcaca agacggtgct tggaaatatc attctgcccc tcttgattca aaaggtggat
    29761 aaggcagaag ctatttcccg agcggataaa attcttgcga ccttccagct gacagctgta
    29821 agagacaagt atcctcatga acttagcggt gggatgcgcc agcgtgtagc cttactccgg
    29881 acctaccttt ttgggcacaa gctctttctc ttagatgagg cctttagcgc cttggatgag
    29941 atgacaaaga tggaactcca cgcttggtat cttgagattc acaagcagtt gcagctaaca
    30001 accctgatca tcacgcatag tattgaggag gccctcaatc tcagcgaccg catctatatc
    30061 ttgaaaaatc gccctgggca gattgtttca gaaattaaac tagattggtc tgaagatgag
    30121 gacaaggaag tccaaaagat tgcctacaaa cgtcaaatct tggcagaatt aggcttagat
    30181 aagtagaaaa atagggagtt ggtgaagatt atcctttacc agcgcccttt ttcttttaaa
    30241 aatgagaaaa tttcggtata atagtcaaac aaggtcaagg tttaaagaga gaggtgggtt
    30301 tgttatgaga tttaaaaata catcggatca tattgaggcc tacatcaagg cgattttaga
    30361 tcaatctggt atcgtggagt tgcaacggag tcagttggca gatacctttc aggttgttcc
    30421 tagtcagatt aactacgtga tcaagacacg ctttacggaa agtagaggct acttggttga
    30481 aagtaagcgt ggtggcggag gctacattcg tataggacgg attgagtttt ctagtcatca
    30541 tgaaatgctc cgggagctgc tttactcgat tggtgagcga gtcagtcaag aaatttatga
    30601 ggatattctc cagcttttgg ttgagcagga attgatgacc aagcaggaga tgaatttgct
    30661 agaatcagta gctttggatc gcgttttagg agaagaagct ccagttgttc gagcaaacat
    30721 gctacgtcag atcatacaag aggtagatag aaaagggaag taagatgaac tattcaaaag
    30781 cattgaatga atgtatcgaa agtgcctaca tggttgctgg acattttgga gctcgttatc
    30841 tagagtcttg gcacttgttg attgccatgt ctaatcacag ttatagtgta gcaggggcaa
    30901 ctttaaatga ttatccgtat gagatggacc gtttagaaga ggtggctttg gaactgactg
    30961 aaacggacta tagccaggat gaaaccttta cggaattgcc gttctcccgt cgtttgcagg
    31021 ttctttttga cgaagcagag tatgtagcgt cagtggtcca tgctaaggtg ctagggacag
    31081 agcacgtcct ctatgcgatt ttgcatgatg gcaatgcctt ggcgactcgt atcttggaga
    31141 gggctggatt ttcttatgaa gacaagaaag atcaggtcaa gattgctgct cttcgtcgaa
    31201 atttagaaga acgggcaggc tggactcgtg aagatctcaa ggctttacgc caacgccatc
    31261 gtacagtagc tgacaagcaa aattctatgg ccaatatgat gggcatgccg cagactccta
    31321 gtggtggtct cgaggactat acgcatgatt tgacagagca agcgcgttct ggcaagttag
    31381 aaccagtcat cggtcgggac aaggaaatct cacgtatgat tcaaatcttg agccggaaga
    31441 ctaagaacaa ccctgtcttg gttggggatg ctggtgtcgg gaaaacagct ctggcgcttg
    31501 gtcttgccca gcgtattgct agtggtgacg tgcctgcgga aatggctaag atgcgcgtgt
    31561 tagaacttga tttgatgaat gtcgttgcag ggacacgctt ccgtggtgac tttgaagaac
    31621 gcatgaataa tatcatcaag gatattgaag aagatggcca agtcatcctc tttatcgatg
    31681 aactccacac catcatgggt tctggtagcg gaattgattc gactctggat gcggccaata
    31741 tcttgaaacc agccttggcg cgtggaactt tgagaacggt tggtgccacc actcaggaag
    31801 aatatcaaaa acatatcgaa aaagatgcgg cactttctcg tcgtttcgct aaagtgacga
    31861 ttgaagaacc aagtgtggca gatagtatga ctattttaca aggtttgaag gcgacttatg
    31921 agaaacatca ccgtgtacaa atcacagatg aagcggttga aacagcggtt aagatggctc
    31981 atcgttattt aaccagtcgt cacttgccag actctgctat cgatctcttg gatgaggcgg
    32041 cagcaacagt gcaaaataaa tcaaagcatg taaaagcaga cgattctgac ttgagtccag
    32101 ctgacaaggc cttgatggat ggcaagtgga aacaggcagc ccagctaatc gcaaaagaag
    32161 aggaagtgcc tgtctataaa gacttggtga cagagtctga tattttgacc accttgagtc
    32221 gcttgtcagg tatccccgtt caaaaactga ctcagactga tgccaagaaa tacttaaact
    32281 tggaagctga actgcacaaa cgtgtcatcg gtcaagacca agctgtttca agtattagtc
    32341 gtgccattcg ccgcaatcag tcaggaattc gcagtcacaa gcgtccgatc ggttccttta
    32401 tgttcctagg acccacaggt gtcggtaaga ccgagttggc caaggctttg gcagaagttc
    32461 tctttgatga cgaatcagcc cttatccgct ttgatatgag tgagtatatg gagaaattcg
    32521 cagccagccg tctaaatgga gctcctccag gctatgtggg ttacgaagaa ggtggggagt
    32581 tgacagagaa ggttcgcaat aaaccctatt ccgttctcct ctttgatgag gtagagaagg
    32641 cccatccaga tatctttaat gttctcttgc aggttctgga tgacggtgtc ttgacagata
    32701 gcaagggacg caaggtcgat ttttcaaata ccattatcat tatgacatcg aacttaggtg
    32761 cgactgccct tcgtgatgat aagactgttg gttttggggc taaggatatt cgttttgacc
    32821 aggaaaatat ggaaaaacgc atgtttgaag aactgaaaaa agcttataga ccggaattca
    32881 tcaaccgtat tgatgagaag gtggtcttcc atagcctatc tagtgaccat atgcaggaag
    32941 tggtgaagat tatggtcaag cctttagtgg caagtttggc tgaaaaaggc attgacttga
    33001 aattacaagc ttcagctctg aaattgttag caaatcaagg atatgaccca gagatgggag
    33061 ctcgcccact tcgcagaacc ctgcaaacag aagtggagga caagttggca gaacttcttc
    33121 tcaagggaga tttagtggca ggcagcacac ttaagattgg tgtcaaagca ggccagttaa
    33181 aatttgatat tgcataaaag aataaaagta ccagcatctg accataagtc acagtggagt
    33241 gaaactcaat gaaaatcaaa gagcaaacta ggcagctagc cgcaggttgc tcaaaacact
    33301 gttttgaggt tgcagataga gctgacgtgg tttgaagaga tttttgaaga gtataagctg
    33361 caagatgaat gattttcttg tattgacgtt gttgttgaca aaaagtagcg gataaatgaa
    33421 atccattcca ttatcataga tgataggctg gtaggaaatt ttcaaatagc atacagaaaa
    33481 tagatgtatg gagttctggt agtagaaagg gagagagatg aacattttag ttgcagatga
    33541 cgaggaaatg attagagaag gaattgcagc atttctgaca gaagagggtt atcatgtcat
    33601 tatggctaag gatggacaag aggtcttgga aaaatttcaa gatctcccta tccatctcat
    33661 ggtactggat ttaatgatgc ctaggaagag tggttttgaa gtgttaaaag aaatcaatca
    33721 aaagcacgat attcctgtca tcgtcttgag tgctctggga gatgaaacta ctcagtcaca
    33781 ggtatttgat ctctatgctg atgatcatgt gacaaaacct ttttctttgg tactgcttgt
    33841 caagcgtatt aaggcgctta tcagacgtta ctacgtcata gaggatcttt ggcgatatca
    33901 ggatgtaaca gtggatttta cctcttacaa agcacattat aaaaatgaag aaattgatct
    33961 caaaccaaag gaattactgg tactaaagtg tttgattcag cataaaaatc aagttttaag
    34021 tagagagcag atattggaag aaatttcaaa agatgtagct gatttacctt gtgatagggt
    34081 cgttgatgtc tatattcgta ctcttcgcaa aaaattagct ttagattgta tcgtgactgt
    34141 gaaaaatgtt gggtataaga ttagcttatg ataaaaaatc ctaaattatt aaccaagtct
    34201 tttttaagaa gttttgcaat tctaggtggt gttggtctag tcattcatat agctatttat
    34261 ttgacctttc ctttttatta tattcaactg gagggggaaa agtttaatga gagcgcaaga
    34321 gtgtttacgg agtatttaaa gactaagaca tctgatgaaa ttccaagctt actccagtct
    34381 tattcaaagt ccttgaccat atctgctcac cttaaaagag atattgtaga taagcggctc
    34441 cctcttgtgc atgacttgga tattaaagat ggaaagctat caaattatat cgtgatgtta
    34501 gatatgtctg ttagtacagc agatggtaaa caggtaaccg tgcaatttgt tcacggggtg
    34561 gatgtctaca aagaagcaaa gaatattttg cttttgtatc tcccatatac atttttggtt
    34621 acaattgctt tttcctttgt tttttcttat ttttatacta aacgcttgct caatcctctt
    34681 ttttacattt cagaagtgac tagtaaaatg caagatttgg atgacaatat tcgttttgat
    34741 gaaagtagga aagatgaagt tggtgaagtt ggtgaagttg gaaaacagat taatggtatg
    34801 tatgagcact tgttgaaggt tattcatgag ttggaaagtc gtaatgagca aattgtaaaa
    34861 ttgcaaaatc aaaaggtttc ctttgtccgt ggagcatcac atgagttgaa aaccccttta
    34921 gccagtctta gaattatcct agagaatatg cagcataata ttggagatta caaagatcat
    34981 ccaaaatata ttgcaaagag tataaataag attgaccaga tgagccgctt attggaagaa
    35041 gtactggagt cttctaaatt ccaagagtgg acagagtgtc gtgagacctt gactgttaag
    35101 ccagttttag tagatatttt atcacgttat caagaattag ctcattcaat aggtgttaca
    35161 attgaaaatc aattgacaga tgctaccagg gtcgtcatga gtcttagggc attggataag
    35221 gttttgacaa acctgattag taatgcaatt aaatattcag ataaaaatgg gcgcgtaatc
    35281 atatccgagc aagatggcta tctctctatc aaaaatacat gtgcgcctct aagtgatcaa
    35341 gaactagaac atttatttga tatattctat cattctcaaa tcgtgacaga taaggatgaa
    35401 agttctggtt tgggtcttta cattgtgaat aatattttag aaagctatca aatggactat
    35461 agttttctcc cttatgaaca cggtatggaa tttaagatta gcttgtagac agattagttt
    35521 tttattaaag ttcatatagg gttaacataa gtgtgttatt ctttgtgtag ataaaagaaa
    35581 ggatactaat atggtattag cgattatttt agtaacattc tttattcgat tgattttttt
    35641 aaagcgttcg atagagaatg agaaacgaat ccttagcaat ggcgggaaag aatttggagt
    35701 tgagaataca aaacgattaa ctatggctca tattgttttt tatctctctt gcttggttga
    35761 ggcaatggtg cacaagacaa tttttgatgg catgggcatg gttggtttgg tcttgcttat
    35821 tttttctatg ctgatgttga tgttggtgat tcacttgttg ggagatattt ggacagtgaa
    35881 gcttatgctt gtcaataatc acaaatatgt agatcatatc ttgtttagga cagtaaaaca
    35941 ccctaattac tttttaaata ttttacctga gttgattggc ttgaccttgt tgagtcatgc
    36001 ttatgtgact tttgttttag tttttccagt ttatgcagtt attttgtatc gacgaatagc
    36061 tgaagaggaa aagctattac atgaagttat aatcccaaat ggaagcataa agagataaat
    36121 acaaaattcg atttatatac agttcatatt gaagtgatat agtaaggtta aagaaaaaat
    36181 atagaaggaa ataaacatgt ttgcatcaaa aagcgaaaga aaagtacatt attcaattcg
    36241 taaatttagt attggagtag ctagtgtagt agttgctagc ttgttcttag gaggagtagt
    36301 ccatgcagaa ggggttagaa gtgagaataa ccccacggtt acatctagtg ggcaagatat
    36361 atcgaagaag tatgctgatg aagtcaagtc acatctagaa aaaatattga gtgagatcca
    36421 aacaaattta gatagaagta aacatatcaa aactgtaaat ctaattaaca aattgcaaga
    36481 cattaagaga acgtatttgt atgaattaaa tgttttagaa gataagtcga aagctgagtt
    36541 gccgtcaaaa ataaaagcag agttagacgc agcttttgag cagtttaaaa aagatacatt
    36601 accaacagaa ccaggaaaaa aggtagcaga agctaagaag aaggttgaag aagctgagaa
    36661 aaaagccaag gctcaaaaag aagaagatta ccgtaactac ccaaccatta cttacaaaac
    36721 gcttgaactt gaaattgctg agtccgatgt gaaagttaaa gaagcggagc ttgaactagt
    36781 aaaaaaggaa gctgacgaat ctcgaaacga gggcacaatt aaccaagcaa aagcgaaagt
    36841 tgagagtgaa caagctgagg ctacaaggtt aaaaaaaatc aagacagatc gtgaaaaagc
    36901 agaagaagaa gctaaacgaa gagcagatgc taaagagcaa gatgaatcaa agaggcgaaa
    36961 gagtcgggta aaacgaggag atcttggaga gccagcaaca cctgataaaa aagaaaatga
    37021 tgcgaagtct tcagattcta gcgtaggtga agaaactctt ccaagcccat ccctgaaacc
    37081 aggaaaaaag gtagcagaag ctgagaagaa ggttgaagaa gctgagaaaa aagccaagga
    37141 tcaaaaagaa gaagatcacc gtaactaccc aaccattact tacaaaacgc ttgaacttga
    37201 aattgctgag tccgatgtgg aagttaaaaa agcggagctt gaactagtaa aagaggaagc
    37261 taagggatct cgaaacgagg aaaaagttaa gcaagcaaaa gcggaagttg agagtaaaaa
    37321 agctgaggct acaaggttag aaaaaatcaa gacagatcgt aaaaaagcag aagaagaagc
    37381 taaacgaaaa gcagcagaag aagataaagt taaagaaaaa ccagctgaac aaccacaacc
    37441 agctccagct ccaaaaccag agaatccagc tgaacaacca aaagcagaaa aaccagctga
    37501 tcaacaagct gaagaagact atgctcgtag atcagaagaa gaatataatc gcttgactca
    37561 acagcaaccg ccaaaaactg aaaaaccagc acaaccatct actccaaaaa caggctggaa
    37621 acaagaaaac ggtatgtggt acttctacaa tactgatggt tcaatggcga caggatggct
    37681 ccaatacaat ggttcatggt actacctcaa cgctaatggt gatatggcga caggatggtt
    37741 ccaatacaat ggttcatggt actacctcaa cgctaatggt gatatggcga caggatggct
    37801 ccaatacaat ggttcatggt actacctaaa cagcaatggt gctatggtaa caggatggct
    37861 ccaatacaat ggttcatggt actacctcaa cgctaacggt tcaatggcaa cagattgggt
    37921 gaaagatgga gatacctggt actatcttga agcatcaggt gctatgaaag caagccaatg
    37981 gttcaaagta tcagataaat ggtactatgt caatggctca ggtgcccttg cagtcaacac
    38041 aactgtagat agctatagag tcaatgccaa tggtgaatgg gtaaactaaa cctaatataa
    38101 ctcgttaatg ctggcttcct gtaagaactc tttaaagtat tccctacaaa taccatatcc
    38161 tttcagtaga taatataccc ttgtaggaag tttagattaa aaaataactc tgtaatctct
    38221 agccggattt atagcgctag agactacgga gtttt
//
LOCUS       DAVLVF010000011        37875 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVLVF010000011 DAVLVF010000000
VERSION     DAVLVF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37875
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.011"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            147..281
                     /gene="rpmH"
                     /locus_tag="VYN66_000527"
     CDS             147..281
                     /gene="rpmH"
                     /locus_tag="VYN66_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV1494591.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            565..1458
                     /locus_tag="VYN66_000528"
     CDS             565..1458
                     /locus_tag="VYN66_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV1494592.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATLEY
                     KLETIPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            1763..2536
                     /locus_tag="VYN66_000529"
     CDS             1763..2536
                     /locus_tag="VYN66_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV1494593.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            2536..3096
                     /gene="rnmV"
                     /locus_tag="VYN66_000530"
     CDS             2536..3096
                     /gene="rnmV"
                     /locus_tag="VYN66_000530"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV1494594.1"
                     /translation="MKEKISQVIVVEGRYDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            3168..4067
                     /locus_tag="VYN66_000531"
     CDS             3168..4067
                     /locus_tag="VYN66_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1494595.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            4387..4683
                     /locus_tag="VYN66_000532"
     CDS             4387..4683
                     /locus_tag="VYN66_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1494596.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            4760..6790
                     /locus_tag="VYN66_000533"
     CDS             4760..6790
                     /locus_tag="VYN66_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1494597.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     LGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            6792..7433
                     /locus_tag="VYN66_000534"
     CDS             6792..7433
                     /locus_tag="VYN66_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494598.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            7426..7719
                     /locus_tag="VYN66_000535"
     CDS             7426..7719
                     /locus_tag="VYN66_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494599.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            7970..8842
                     /gene="rsmA"
                     /locus_tag="VYN66_000536"
     CDS             7970..8842
                     /gene="rsmA"
                     /locus_tag="VYN66_000536"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV1494600.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLMPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            8844..9722
                     /gene="rsgA"
                     /locus_tag="VYN66_000537"
     CDS             8844..9722
                     /gene="rsgA"
                     /locus_tag="VYN66_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV1494601.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            9733..10389
                     /locus_tag="VYN66_000538"
     CDS             9733..10389
                     /locus_tag="VYN66_000538"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1494602.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            10352..11014
                     /locus_tag="VYN66_000539"
     CDS             10352..11014
                     /locus_tag="VYN66_000539"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV1494603.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLLLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            11016..12272
                     /locus_tag="VYN66_000540"
     CDS             11016..12272
                     /locus_tag="VYN66_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV1494604.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            12262..13203
                     /locus_tag="VYN66_000541"
     CDS             12262..13203
                     /locus_tag="VYN66_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV1494605.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            13470..14297
                     /gene="purR"
                     /locus_tag="VYN66_000542"
     CDS             13470..14297
                     /gene="purR"
                     /locus_tag="VYN66_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV1494606.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            14364..15614
                     /locus_tag="VYN66_000543"
     CDS             14364..15614
                     /locus_tag="VYN66_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV1494607.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            15740..16534
                     /gene="pflA"
                     /locus_tag="VYN66_000544"
     CDS             15740..16534
                     /gene="pflA"
                     /locus_tag="VYN66_000544"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV1494608.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(16609..17535)
                     /gene="yidC"
                     /locus_tag="VYN66_000545"
     CDS             complement(16609..17535)
                     /gene="yidC"
                     /locus_tag="VYN66_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1494609.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(17613..17891)
                     /locus_tag="VYN66_000546"
     CDS             complement(17613..17891)
                     /locus_tag="VYN66_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV1494610.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            17925..18665
                     /locus_tag="VYN66_000547"
     CDS             17925..18665
                     /locus_tag="VYN66_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV1494611.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            18721..19404
                     /locus_tag="VYN66_000548"
     CDS             18721..19404
                     /locus_tag="VYN66_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV1494612.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            19517..20509
                     /locus_tag="VYN66_000549"
     CDS             19517..20509
                     /locus_tag="VYN66_000549"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV1494613.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            20574..21113
                     /gene="rsmD"
                     /locus_tag="VYN66_000550"
     CDS             20574..21113
                     /gene="rsmD"
                     /locus_tag="VYN66_000550"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV1494614.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            21103..21591
                     /gene="coaD"
                     /locus_tag="VYN66_000551"
     CDS             21103..21591
                     /gene="coaD"
                     /locus_tag="VYN66_000551"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV1494615.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGRDIACYVPESILEEIRNEK
                     KD"
     gene            21575..22612
                     /locus_tag="VYN66_000552"
     CDS             21575..22612
                     /locus_tag="VYN66_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV1494616.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     INDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            22670..22900
                     /locus_tag="VYN66_000553"
     CDS             22670..22900
                     /locus_tag="VYN66_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV1494617.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDENTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            22924..24207
                     /gene="murA"
                     /locus_tag="VYN66_000554"
     CDS             22924..24207
                     /gene="murA"
                     /locus_tag="VYN66_000554"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1494618.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            24200..24388
                     /locus_tag="VYN66_000555"
     CDS             24200..24388
                     /locus_tag="VYN66_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1494619.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            24427..25251
                     /gene="endA"
                     /locus_tag="VYN66_000556"
     CDS             24427..25251
                     /gene="endA"
                     /locus_tag="VYN66_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV1494620.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            25480..26811
                     /locus_tag="VYN66_000557"
     CDS             25480..26811
                     /locus_tag="VYN66_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV1494621.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTNTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRIAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(27009..27079)
                     /locus_tag="VYN66_000558"
     tRNA            complement(27009..27079)
                     /locus_tag="VYN66_000558"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27045..27047),aa:Cys,seq:gca)
     gene            27495..31106
                     /gene="rpoB"
                     /locus_tag="VYN66_000559"
     CDS             27495..31106
                     /gene="rpoB"
                     /locus_tag="VYN66_000559"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1494622.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TSEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            31139..34816
                     /gene="rpoC"
                     /locus_tag="VYN66_000560"
     CDS             31139..34816
                     /gene="rpoC"
                     /locus_tag="VYN66_000560"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV1494623.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGAIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            34927..35340
                     /gene="ndk"
                     /locus_tag="VYN66_000561"
     CDS             34927..35340
                     /gene="ndk"
                     /locus_tag="VYN66_000561"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV1494624.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(36269..36901)
                     /locus_tag="VYN66_000562"
     CDS             complement(36269..36901)
                     /locus_tag="VYN66_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494625.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(36903..37670)
                     /locus_tag="VYN66_000563"
     CDS             complement(36903..37670)
                     /locus_tag="VYN66_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1494626.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
BASE COUNT        11164 a         7270 c         8715 g        10726 t
ORIGIN      
        1 agggcttgca ttcgaaaaag ctggaaacat ttgcctagag ctattgacaa aagtgaaaga
       61 atcagataga atagtaaagt atgtttagta actgatcact ttatagccgg ctaaacgaat
      121 acaaaatcta tgaggaggta ttcatcgtga aacgtactta tcaaccaagt aaacttcgtc
      181 gtgcgcgtaa acacggattc cgtaaccgta tgtcaactaa aaatggtcgt cgcgtattgg
      241 cagctcgtcg tcgtaaagga cgcaaagttt tggctgcata atccaaacga attctatcaa
      301 aaatcagtag gaactcgagt ctactgattt ttatttttgt aaaaaagttc agtagatgca
      361 aatggattcg gaagcgatgt tatagtagat tgaaactaga atagtacacc tctgtttcta
      421 aaacattgtt agaaatcgat ttgactgtcc tgatcgattt gtcctgttat tattttattt
      481 tactataaag ttgaagtagg tggagatggt acagcaacaa tcgtctttaa agatggttca
      541 gctattacaa ttccaggaaa tcaattggta gcacaagatc caaaagcaca agatagcact
      601 aaaccgactg ctgaaaaatc aactgttaaa gcacctgctc aaagagtaga tgtaaaagat
      661 ataactcatt taacagatga agaaaaagtt aaggttgcta ttttacaagc aaatggttca
      721 gcattagacg gagcgacaat caatgtagct ggagatggta cagcaacaat cacattccca
      781 gatggttcag tagtgacgat tctaggaaaa gatacagttc aacaatctgc gaaaggtgaa
      841 tctgtaactc aagaagctac actagagtat aagctagaaa ctataccagg tggagataag
      901 ggaggcaata ctggaagctc agatgctaat gcgaatgaag gcggtggtag ccaggcgggt
      961 ggatcagctc acacaggttc acaaaactca gctcaatcac aagcttctaa gcaattagct
     1021 actgaaaaag aatcagctaa aaatgccatt gaaaaagcag ccaaggataa gcaggatgaa
     1081 atcaaaggcg caccgctttc tgataaagaa aaagcagaac ttttagcaag agtggaagca
     1141 gaaaaacaag cagctctcaa agagattgaa aatgcgaaaa ctatggaaga tgtgaaggaa
     1201 gcagaaacga ttggagtgca agccattgcc atggttacag ttcctaagag accagtggct
     1261 cctaatgctg ctcctaagac aacaagtgca ccgcaagcaa ctgcaggaac aatgcaagat
     1321 gttacctacc agtcacctgc tggcaaacaa ttacctaaca caggttcagc atcaagtgca
     1381 gcacttgcta gtcttggtct agtggtggca acaagtggtt ttgctttgct aggaagaaag
     1441 actagacgta gaaaatagaa cagctagaaa attctattct ctacttaaag ttagattata
     1501 agggggattt tgagaagtca tcaatcctag tgatgggtga gaaaagtgag aacccaagat
     1561 aatcacatac tttagctgaa taggaatatt ctatcaatgt agccaatctc ttctgtctct
     1621 aactgtggaa taggagatgg gcaatatcgg atagaaaaga tagcagaata gctctctatt
     1681 gaagagagga ggggaaaccg aaaaattagg tgcccctcct cttttttggt ataatagaag
     1741 atagaaaacg aggttagaag agatgatttt tgatacacat acacacttga atgtagaaga
     1801 atttgcaggt cgtgaggcag aagaaattgc cttggctgct gagatgggtg tgatacagat
     1861 gaatattgtt ggttttgata aaccgacgat tgagcgtgcc ttggagttgg tagatgagta
     1921 tgagcagctc tatgcgacta ttggttggca tcctacagaa gctggtactt atacagagga
     1981 agttgaggct tacttgttgg ataagttaaa acattccaag gttgtggctt taggtgaaat
     2041 tggcttagac taccattgga tgacagcgcc caaagaggtg caggagcagg tttttcgccg
     2101 tcagattcag ctatctaagg acttggattt gccttttgtt gtccataccc gtgatgcgct
     2161 ggaagatacc tatgagatta tcaagagtga gggcgttggt cctcgtggtg gtatcatgca
     2221 ttcattttca gggacgcttg agtgggcaga gaagtttgtg gatcttggta tgaccatttc
     2281 cttctcagga gtggtgactt tcaagaaggc aactgacctc caagaagcag ctaaagagtt
     2341 acctttggac aagatgttgg tggaaacaga tgcgccttac ttagcacctg tacccaagcg
     2401 tggtcgtgaa aataaaacag cctatactcg ctatgtggtc gactttatcg ctgacttgcg
     2461 tggtatgacg acagaagagc tggcggtagc aacgactgca aatgcagaac gaatttttgg
     2521 attggacagc aagtaatgaa agagaaaatt tctcaagtta tcgtggttga aggtcgctat
     2581 gatacggtca atctcaagcg ttatttcgat gtggagacct atgagacacg aggttctgcc
     2641 atcaatgatc aggatataga gcgaattcag cgtctacacc aacgtcatgg agtcattgtc
     2701 tttacagacc cagattttaa tggggagcgg attcggcgca tgattatgac ggccattcca
     2761 acagttcagc atgcctttct caagcgagat gaagctgttc ccaagtccaa gaccaagggg
     2821 cgttctctgg gaattgagca tgccagctat gaagacctga aaacggctct agctcaggtg
     2881 acagaacaat ttgaacatga gagtcagttt gacattagtc gtagcgactt gattcgcctt
     2941 ggttttctag caggggcaga cagccgtaag cgtagagaat atctcggaga gactctccga
     3001 atcggctatt ctaatggcaa gcaactcctc aaacgcctag agttgtttgg ggttactttg
     3061 gcagaagtgg aagaagctat gaaatcttat gagtaggaaa gatgtagccg ttacaatttt
     3121 ttaagtttca cagtattttt cgaagcaggt agaagaggag gcgtctgatg ttaattggtc
     3181 aaaaaattaa agagattcgg atagaaaaag gaattagtcg tccagatttt tgtggagatg
     3241 agcaagaact gacagttcgt caactgtcgc gaattgaaag tggagcttcg caaccgagtt
     3301 tgcccaagtt agcctatatt gctcgccggc taggagttcc agtttatagc cttatgccgg
     3361 atttttcagc tcttccttct gcttatttag aattgaaata ccagatttta cgtgaaccaa
     3421 tctatggtaa agaagaggag tatgataaga aagaagcgtg tttggaagag atttataaaa
     3481 catactttga taatcttcct aaagaagaac aattagcatg tgaagtattg caggcgtgtt
     3541 tggatacttc tagaactaga aggcctgaat atgcagagtt aatacttgag gaacatatgt
     3601 ctcagatcat agaaaaagaa gtttattcaa caaatgatat gttgttgatt cgtttgtttt
     3661 tttatcaaat gctcattaga aaagatcttg ccaaatttat aaatcaaatc gaaaagctaa
     3721 tgctctttct tttggaacag aaaaaggtaa ctaaattaga aaacttcttt ataattagag
     3781 atactcttat ttcaggaatg tgttgtcttg aaaaggtagg agtaactgat tgttttaatg
     3841 attatctatc gtgtttacaa gaaattatgg ataaaactca agattatcaa aagaaacctc
     3901 ttgtatttat gtttttgtgg aagcaagcat taagagtaga aagagatttt agtttagctg
     3961 aatcatttta tcagtcttct aaaacatttg cgaagctaat tggagatgga tttctagtaa
     4021 agaaattgac agaggaatgg caagaggatg tcaaaaaata tttataaaca tagtgaatca
     4081 gtgacaaaaa tgtccttgtc ctcgtatcaa aacagttcta aagttcgtct ttagaactgt
     4141 ttttttagat ataagctaaa aataatatga aatagttaga ttttaaggac attgatgtcc
     4201 ttgatagtgt gataaagatg gagtataatg actataaaag ttcatttcag ggtgctattt
     4261 tgacaattta gagaaaaaca aataatttta gaatatcatt tggtaatata atagtatcaa
     4321 cgttgataat ggaaagagga gcaagtaact caacaatctt gtaatcaaaa atagaggagt
     4381 atcaatatga aacaaacagt taaaaagtta gctcttgtag cgagcattgc agcaacatta
     4441 ggtggaggtg tatcagttgc ctctgcagcc gttcaatacc cagaaggtgg tgtttggact
     4501 tatggttcag gtaacggtgg tgcttactca aactactatc acccttcaaa ataccatagt
     4561 tcaacagttg ttagcagaaa aactggttca tctgacaagg gatatgctgg tgctggaggg
     4621 acttctcgtg catggattcg tacttcttgg ggagagaaag ttgcattcta ctataatgtt
     4681 tagaatggta tagccctatt gtttagcttt acttggtcac cttctcacta agtgaccaag
     4741 taactttttg gaggaaatga tgaaacgttt atttattttg atttcaatgg tattggtatc
     4801 gctttatatg gtgataactt ccgttgacca tcgagaagag attttatttg gtaactatcc
     4861 ttctgttgat gtgacaggaa tgatgataaa tcaaccagta gctagtcgcg aagaggtgac
     4921 agaggctttg agtcacttgg cggtagagca taatagtctc atcgcccgtc gaatcgttga
     4981 gccaaatgaa gctggagaaa cactctttac ctatgccact tatggtgagg gagagcttcc
     5041 agaaggtctg accatttcct ccaaggagag tgcagaaacg agtgatttat tagggtctta
     5101 cttgattgtg tcaggaagtt tggatggagt gagcttacag accaccttga aagagcttgg
     5161 ttatcaaggc tttgtttcga atggagaaga tccattttcg atagtcttac tattggcggc
     5221 cacccctatg gtgctactga gtttagctat ttttctgctg acctttatga gtctgaccct
     5281 gatttatcgg atcaaatccc ttcgtcaggc agggattcgc ttaatagctg gtgagagctt
     5341 gtttggagtt gctctcagac cagtgttaga agatgtgaga cagcttatct gctcagtgct
     5401 ggtatccagt cttttgggat tggggattct ctggtatcaa ggtgccttgt ttatggcaac
     5461 ggtacaactg gtcatcattg ctcttctact ttatggattg accttggcag ggatttctac
     5521 cttactaagt gtcgtctatc tacttggttt acaggaaaat agtctggtgg atctattgaa
     5581 agggaaactc cctctcaaac gtatgatgac attgatgatg gtggggcaac tcttagctgt
     5641 attggtggtc ggatcgagtg cgacagctct cctaccccac taccgtgaaa tgcaggaaat
     5701 ggagagagct agcaataaat ggagccagtc ctcagaccgt taccgtctat cctttggttg
     5761 gtctagtgca tttgccgatg aagaaggaac gcgtaaggat aatcgtgagt ggcagacatt
     5821 tactgaagaa cggttagcca atacagactc tttttatatt atgagcaatg ttgacaattt
     5881 ctcagatgga gcagaagtgg acctagatgg caatcgtctc agtgactaca caccgttagg
     5941 gaatgttatc tatgtctcgc cgcgctatct gatagaagaa aagattaccg tttcttcaga
     6001 gtttatggac aagatgcaaa acttgtctga gggagagttt gggctgatct tgcctgagag
     6061 cttgcgagag cagtctgcct actaccaagg attgtttaca gattacctgc aaaacttttc
     6121 atctgaaagt gtagaagtga cgagtcagaa acactacctc ccacaggtaa ggctagcttt
     6181 tacagaaaca ggacaggaac gtttcctcta taatgatggg tacaagacaa cacgccagta
     6241 cctaaaagat ccgattattg tagttctaac gccgcaagcg actggaacaa gacctgttgc
     6301 agggatgttg tggggaacta cggctaatag tgccttgaaa ctagatcgat atggagacag
     6361 catcacagct ctaaaagagc aaggtctgta tcacaaggtt tcttacttgg taaaaagcca
     6421 gctatttttt gccaaggtac taaatgacaa acgggtggag ttttactctc tccttattgg
     6481 gacgattttg accctgtcta cggctatctt gttatttgat tccatgaatc ttctctattt
     6541 tgagcagttc agacgggaac ttatgattaa acgtcttgct ggtatgacaa tctatgagct
     6601 tcatggcaag tatttactgg cgcaaggagg agttctcttg cttggcctag tcctatctag
     6661 tattttgaca agagatggtt tgattagcgc tctagttgta gctttgttta cgcttaacgc
     6721 cctcttgatt ttagtaaggc aggataaaaa agaagaagct ggtagcatgg cagtattgaa
     6781 aggaaaataa gatgattgat attcaaggat tggaaaagaa atttaatgac cgcgcgattt
     6841 tctctggttt gaatctcaag ctggagaagg gcaaggttta tgccttaatc ggaaagagtg
     6901 gaagcggaaa gacgacgctg ctgaatatct tgggaaagct agaaaagata gatggtggaa
     6961 gggttctcta tcaggggaaa gatttaaaaa ccattcctac tcgtgagtat tttcgagacc
     7021 agatgggcta tctctttcaa aatttcggcc tcttagaaaa ccaatcaatc aaagaaaatt
     7081 tggatttggg ttttgttggt cagaaaatct caaaagtaga acgtttggaa aggcaagtgg
     7141 gggctttaga aaaagttaat ctagggtatt tggatttaga acaaaaaatt tatactttat
     7201 ctgggggaga ggcccaacga gttgcccttg ctaagactat tttgaaaaat ccacccttga
     7261 ttttggcaga tgaaccaaca gcagctcttg atcctgaaaa ttcagaggag gttatgaatc
     7321 tcttggtgga tttgaaagat gaaaatcgaa ttatcatcat tgcgacccat aatcccctag
     7381 tctggaataa ggctgatgaa atcattgata tgaggaaact tgctcatgtg tgaaaaaatc
     7441 cgtattcgca gggtatctga ttatcctagt gccagaggtg gtttagaaga tatcctcatc
     7501 atggaaaata tgaccaatca tctccttttg gttcaaatcc gagtgcatgg ctatttgctt
     7561 gattttgcta gtattgaagg gcaaaggcaa aagcattatc gtttgaaaaa tttacctcag
     7621 acggttgaac tgacagtgga tgatgtggag gaggatgtgg atttgaccct acccgaaaat
     7681 cgaagttatc aagaagctga tttttttgat actcaatgaa aatcaaagag caaactagga
     7741 agctagccgc aggctgtact tgagtacggc aaggtgaagc tgacgtggtt tgaagagatt
     7801 ttcgaagagt atgaacgtat gtttcgagag aagcactaag tccctcttta aagatttcca
     7861 agactatctt tcttcatgag gaaagatagt tttttggtat gattttcatt cccaaaatac
     7921 aaggggaatg tgttacaata gtagtaacag ataatagaaa agagaataga tgagaattgc
     7981 agattatagc gtgaccaagg cagtgttgga gcgtcacggt tttaccttta aaaagtcctt
     8041 tgggcaaaat ttcttgacgg ataccaatat ccttcaaaaa attgtggata cggctgaaat
     8101 tgatgatcag gtcaatgtca tcgaaatcgg gccaggtatt ggtgccttga cagaattttt
     8161 ggctgagcgt gcagcccaag tcatggcttt tgagattgac caccgtttga tgccaatttt
     8221 ggcagatact ctgcgtgatt ttgataatgt gaccgtagtt aacgaagata ttctcaaggt
     8281 tgatttggcg caacatatcc aaaattttaa aaatcctaac ctgccaatca aggtagtggc
     8341 taatttgcct tactacatca cgacgcctat tctcatgcac ttgattgaga gtggcattcc
     8401 tttttgtgag tttgtggtca tgatgcagaa agaagtagcg gaccgcattt cagcccagcc
     8461 taacaccaag gcttacggta gcttgtctat cgccgtgcag tattacatga cagccaaggt
     8521 tgcctttatc gtgcctcgta cggtctttgt gccagcgcca aatgtggatt cagccatctt
     8581 gaaaatggtg cgtcgtccag agccagccgt agcagtagaa gatgagaact ttttctttaa
     8641 ggtttccaag gctagtttta cccatcgccg caagaccttg tggaataact tgacaggtta
     8701 ctttggtaag actgaagagg tcaaggacaa gctgaccaag gctttggacc aggcaggctt
     8761 gtcaccaagt gtgcgtgggg aagctctcag cttggcagaa tttgccggtc tagcagacgc
     8821 acttaaaggg caaggactct aagatgcagg gacaaatcat taaagccttg gcaggtttct
     8881 actatgtgga gagtgatggc caggtttatc aaacacgcgc gcgtgggaat ttccgtaaaa
     8941 aaggccatac cccttatgtt ggggactggg tagatttctc tgccgaggaa aattcagaag
     9001 gctatatcct caaaattcac gaacggaaaa acagtctggt tcgtccgcct attgtcaata
     9061 tcgatcaagc tgtagtaatc atgtccgtca aggaacctga ttttaacagc aatttgctgg
     9121 atcgtttctt ggttcttttg gagcacaagg gcatccatcc cattgtctat atttccaaaa
     9181 tggatttgtt ggaagatagg ggagaactgg atttttacca gcagacctat ggtgacatcg
     9241 gctatgactt tgtgaccagt aaagaggaac tcctgtcttt gttaacaggc aaggttacgg
     9301 tctttatggg gcagacaggt gttgggaagt caactcttct caataaaatc gcaccagacc
     9361 tcaatcttga aacgggagaa atttcagaca gtctaggtcg cggtcgccat accactcgag
     9421 ctgttagttt ttacaatctc aacgggggta aaatcgcaga tacaccaggc ttttcatctc
     9481 tggactatga agtatcaagg gctgaagacc tcaatcaggc ctttccagag attgctactg
     9541 ttagccgaga ttgtaagttc cgtacttgta cccataccca tgagccgtct tgtgccgtca
     9601 aaccagctgt tgaagagggt gttattgcaa ccttccgttt tgacaattac ctgcaattcc
     9661 ttagtgaaat tgaaaatcgt agagaaacct ataaaaaagt cagcaaaaaa attccaaaat
     9721 aaggagaaac ctatgtctca atacaagatt gctccgtcaa ttctggcagc agattatgcc
     9781 aactttgaac gtgaaatcaa acgtctagaa gcaactgggg cagaatatgc ccatatcgat
     9841 atcatggaca gtcattttgt accgcaaatc agttttggtg caggtgtggt cgagagcctt
     9901 cgtcctcata gtaagatggt tttcgattgc cacttgatgg tgtcaaaccc tgagcatcat
     9961 ctggaagatt ttgcgcgtgc aggtgcagac atcatcagta tccatgtaga agcaacgcct
    10021 catattcatg gcgccctcca aaaaattcgt tcactcggag ttaagccttc agtcgttatc
    10081 aatcctggca catcagttga agccatcaag cacgtccttc atctagttga ccaagtttta
    10141 gtcatgacgg ttaatccagg ttttggtggg caagcctttc tgccagaaac catggataag
    10201 gttcgtgagt tggttgctct tcgtgaggaa aaaggtttga actttgaaat cgaagtggat
    10261 ggtgggattg atgaccaaac tattgcccaa gccaaagaag ctggtgcgac cgtttttgta
    10321 gcaggttcct atgtctttaa gggagatgtc aatgagcgag tacaaactct cagaaaacaa
    10381 ctggactagg gttgccgttt ttgcaggcgg agaccgcggt cattatcgga cagattttga
    10441 tgcttttgtt ggggtggatc gaggctcgct ctgggtcttg gaagaagact tgcttcttgc
    10501 tctagcagtt ggggattttg attctgtgac cgaagaagag cgacaggtga ttcaaaaacg
    10561 tgcccagcat tttgtccaag cccgaccaga aaaagatgat accgatttgg aattggctct
    10621 cttaaccatc tttgagaaaa atcctcaggc tcaggttact attttcggtg ccttgggtgg
    10681 ccgtattgac catatgttgg ccaatgtctt tctgcctagc aatcctaagt tggcacccta
    10741 tatgcatcaa atagaaattg aggatgggca aaacttgatt acttattgtc cagaagggac
    10801 cagtcagcta gaacctcgtt cagactacga ctatctagcc tttatgccag ttcgggatag
    10861 ccagctgact attcttggag ccaagtatga gttgacagag gaaaattttt tctttaaaaa
    10921 agtgtacgct tctaacgaat atatagatag ggaagtgtcg gtaacttgcc cagatggtta
    10981 tgtggtcgta ctgcatagca aggacaggag gtaggatgga aagtttactt attctattat
    11041 taattgccaa cctagctggt ctctttctga tttggcaaag gcaggatagg caggagaaac
    11101 acttaagtaa gagcttggag gatcaggcag atcatttgtc agaccagttg gattaccgct
    11161 ttgaccaagc cagacaagcc agccagttag accaaaaaga tttggaagtg gttgtcagcg
    11221 accgtttgca agaagtgcgg attgaattgc accaaggtct gacccaagtc cgtcaagaaa
    11281 tgacagataa tctcctccaa actagagaca agacagacca acgtctccaa gccttgcagg
    11341 aatcaaatga gcaacgtttg gaacaaatgc gccagacggt cgaggaaaaa ctagaaaaga
    11401 ccttgcagac acgcttacag gcttcctttg agacagtttc taaacaactg gagtctgtca
    11461 atcgtggcct tggagaaatg cagacagttg cccgtgatgt cggagctctt aacaaggttc
    11521 tctctggaac caagacgcga gggattctgg gagaattgca actggggcaa attattgaag
    11581 acatcatgac acctgcccag tacgaacgag aatacgcaac ggttgaaaac tctagtgaac
    11641 gagtggagta tgccatcaag ttacccggac aaggcgacca agaatacgtc tatctgccaa
    11701 ttgactctaa gtttccactg gcagattatt accgcttgga agaagcctat gagacaggtg
    11761 acaaggatga gattgaacgc tgtcgtaagt cactcctagc aagcgtcaag cgctttgcca
    11821 aggatatcaa gagcaagtac atagcaccgc ctcggacgac caattttgga gttttgtttg
    11881 ttccgacaga aggtctttat tcagagattg tgcgcaatcc ggtcttcttt gatgatttga
    11941 gacgggaaga acagattatc gttgcaggac caagtaccct atcagccctt cttaactccc
    12001 tatcagttgg tttcaagacc cttaatatcc aaaagagtgc cgaccatatc agcaagactc
    12061 ttgccagtgt caagaccgag tttggcaagt ttggtggtat tctggtcaag gcacaaaaac
    12121 atctccaaca tgcctctggc aatattgatg aattattaaa ccgtcgtacc atagctatcg
    12181 agcggacgct ccgtcacatt gagttgtcag aaggcgagcc tgcgcttgat ctactccatt
    12241 ttcaagaaaa tgaggaagaa tatgaagatt agtcacatga aaaaagatga gctatttgaa
    12301 ggcttttacc taatcaaatc agctgacctg aggcaaactc gagctgggaa aaactaccta
    12361 gcctttacct tccaagatga tagtggcgag attgatggga agctctggga tgcccaacct
    12421 cataacattg aggcctttac cgcaggtaag gttgtccaca tgaaaggacg ccgagaagtt
    12481 tataacaata cccctcaagt caatcaaatt actctccgcc tgcctcaagc tggtgaaccc
    12541 aatgacccag ccgatttcaa ggtcaagtca ccagttgatg tcaaggaaat tcgtgactac
    12601 atgtcgcaaa tgattttcaa aattgaaaat cctgtctggc aacggattgt ccgaaatctc
    12661 tacaccaagt atgataagga attctactcc tatccagctg ccaagaccaa ccaccatgcc
    12721 tttgaaacgg gcttggccta tcatacggcg accatggtgc gtttggcaga cgctattagc
    12781 gaagtttatc ctcagctcaa taagagcctg ctctatgcgg ggattatgtt gcatgactta
    12841 gctaaggtca tcgagttgac ggggccagac cagacagagt acacagtgcg aggtaatctt
    12901 cttggacata tcgctctcat tgatagcgaa attaccaaga cagttatgga actcggcatc
    12961 gatgatacca aggaagaagt cgttttgctt cgtcatgtca tcctcagtca ccacggcttg
    13021 cttgagtatg gaagcccagt ccgtccacgc attatggaag cagagattat ccatatgatt
    13081 gacaatctgg atgcaagcat gatgatgatg tcaacagctc ttgctttggt ggataaagga
    13141 gagatgacca ataaaatctt cgctatggat aatcgttcct tctataaacc agatttagat
    13201 taataattta agaaaaatga gcatttttta ggataagaat gttcgttttt ttatgtgaat
    13261 atggtataat aagtaaaaga caaaaatgaa tactcttcga aaatctcttc aaactagggt
    13321 agtatcgcct tgtcgtatgt atatatgcag gtatattaca gggtttgtca gttctattga
    13381 caatctcaaa acagtgtttt gaaccaccag cgaccagctt tctagtttgc tttttgattt
    13441 tttgaataaa aatggaatag gaaatagaaa tgaaattaag aagaagtgat cggatggttg
    13501 tcatttccaa ctatttgatt aataatcctt ataaactaac tagtctcaat acttttgctg
    13561 aaaagtatga gtctgctaaa tcatccatct cagaagatat cgtcattatc aaacgcgcct
    13621 ttgaggaaat tgaaatcggt catatccaga cagtgactgg ggctggcgga ggtgtcatct
    13681 tcacaccgtc tatttcgagt caggatgcta aggaaatggt tgaagacttg cgtaccaagt
    13741 tgtcagaaag tgaccgtatc ttgccaggtg gttatatcta tctgtctgat ttgcttagca
    13801 caccagccat cttgaaaaat attggtcgta ttattgccaa aagctttatg gaccaaaaaa
    13861 ttgacgcggt tatgaccgta gcaactaagg gtgtgccact tgcaaatgca gttgccaatg
    13921 tcctcaatgt ctcttttgtc attgtgcgcc gtgacctgaa aattaccgaa ggttcaactg
    13981 ttagcgtcaa ctatgtttca ggttcaagtg gtgaccgtat cgagaaaatg ttcctttcaa
    14041 aacgtagtct taaggcaggt agccgtgtct tgattgtgga tgacttcttg aaaggtggcg
    14101 gaacggtcaa cggtatgatt agtctcttgc gcgagttcga ctcagaactg gcaggtgtag
    14161 cggtctttgc ggacaatgcc caagaagaac gtgaaaagca gtttgactac aagtcactct
    14221 tgaaggtaac taatattgat gtcaagaacc aagccatcga tgttgaggtt ggcaatatct
    14281 ttgacgaaga taaataagag atagaactaa aggttggaac gattgtccca gcctttcttt
    14341 gcaaacagaa tagaaggaag cttatgaaaa caccatttat caatagagaa gagttagaag
    14401 cgattgttgc cgagttcccg actccctttc acttgtatga tgagaagggg attcgtgaga
    14461 aggcaagagc cgtcaaccaa gctttttcgt ggaacaaggg ctttaaggaa tattttgcag
    14521 ttaaggctac tccaactcca gctattttga aaattctcca agaagaaggt tgtggtgtgg
    14581 actgctctag ttatgtagag cttttgatga gccataaact ggactttctg ggttctgaga
    14641 ttatgttctc ttccaacaac acgccagaca aggaatacgc ctatgcacgt gaattgggtg
    14701 cgaccattaa cttggatgcc tttgaagata ttgaacatct ggagagagta gcaggcattc
    14761 cagaaatcat ctcttgtcgt tataatcctg gaggcgtttt tgaactgggg acagacatta
    14821 tggacaatcc tggggaggct aagtttggca tgaccaagga ccagctcttt gaagcctttg
    14881 ctatcttgaa ggaaaaagga gccaagactt ttgggattca ctccttccta gcgtccaata
    14941 ccgtgaccca tctctattat ccagagttgg ctcgtcagct ctttgaactg gctgttgaaa
    15001 tcaaggaaaa gttgggcatt tggctagact ttatcaatct ttctggcggt attggtgtta
    15061 attatcgtcc agaccaggag ccgaacgata tcgccttgat tggtgaggga gttcgtaagg
    15121 tgtatgaaga agttcttacg tcagcaggtc ttggtcaggt caagattttc accgaattgg
    15181 gtcgttttat gctggcacct cacggtgctc tagtcacaag agtgactcat aagaaggaaa
    15241 cctaccgtac ctatctaggt gtggatgcct cagcagtcaa cctcatgcgt ccagctatgt
    15301 acggagctta ccatcatatt agcaacgtga cccatccaga tggaccagct gaagcggtag
    15361 atgtggttgg ttcactctgt gaaaacaatg ataaatttgc agtcaatcgc gaactgcctc
    15421 atacagaaat cggtgatttg ctggtaattc atgatacagg tgcccacgga ttttcaatgg
    15481 gctaccagta taatgccaaa ttacgttctg cggaaatcct ctataccgaa gaaggtaaag
    15541 cccgtcaaat ccgccgtgca gagcgccctg aggactattt tgcaacctta tatggcttcg
    15601 attttgaaga ataatctgat aatagattga aaatgaaatt gaaaaacaga ttgctttcta
    15661 aaaaataggc aaaaatcttg tttttccttc aagtcgtgat ataataaaac tataaaacgt
    15721 tttcaaggaa ggtaacgata tgtctgaaga aacaattgat tatggacaag tgacaggaat
    15781 ggtgcattcg acagaaagct ttgggtcagt agatgggcct ggtattcgct ttattgtctt
    15841 tttgcagggc tgtcacatgc gttgccagta ttgccacaac ccagacactt gggctatgga
    15901 gtccaataag tcacgtgaac ggacggtaga tgatgtcttg acagaggcct tgcgctaccg
    15961 tggtttctgg ggaaataagg gtgggattac agtcagtgga ggagaagctc tcttgcagat
    16021 tgatttcctg attgctctct tcaccaaggc taaggaacaa ggaatccact gtaccttgga
    16081 tacctgtgct cttcctttcc gtaataaacc acgttacctt gagaagtttg acaaactcat
    16141 ggctgtcact gacttggttc ttttggatat caaggaaatc aacgaagaac agcacaagat
    16201 tgtcactaga caaaccaata aaaatatctt ggcttgtgcc cagtatctat cagatattgg
    16261 aaaacctgtc tggattcgcc acgtgctagt tccaggattg acagacagag atgatgactt
    16321 gattgaactt ggtaagttcg tcaagaccct caaaaatgtt gataagtttg aaattctacc
    16381 ttatcacacc atgggtgagt tcaagtggcg tgaacttgga attccatatt ccctcgaagg
    16441 agtcaaacca ccaacagcag atcgcgtcaa gaacgctaaa aaactcatgg ataccgaaag
    16501 ttatcaagat tatatgaaac gtgtacatgg atagaaaaga agcctgatgg aaacatcggg
    16561 cttttgattt gcaaaaagac ttagcaaatc agctaagcct ttttcttctt atctcgaacg
    16621 ttgttttcca gcgttgcgat ttttgtgttt tttcttgctt gtgatagcag ttggttgttc
    16681 aggggtaacg tcttttcgtc cacttggttt agagaaagca cttgcttttg gtgggttctt
    16741 ggctagttct tcacggactt ttttgcgaag ttttggacga acgatatagt tgacgataaa
    16801 ctgttggaga atcatcatga aaccaccgac aacccagtaa agtgtgacac tagctggtga
    16861 gaagagggag aagacgacga tcatgagtgg gctcatgtaa atcattttct tgatttgttc
    16921 tctttgcatt tcatcttcta ctccgtgaag tgaaaggagc gattgaagat agtaaaggac
    16981 accagcacag gcaaccaaaa tcatacttgg agaacctaga ggaatgccta ggtagcttgc
    17041 ttgagcaacc ccttcagtat gttgggcagc aaagtagata gcagagaaga aaggcatttg
    17101 aaggaggata gggaaacatc ctacaccgcc aaacatgctg ataccgtgct ctttttgagc
    17161 agcaaagaga gcttgttggg cttcgagttt ttcttcttga gtagtcgctt ctttgagacg
    17221 cgtttggtgt ggctcaagga cgtgcttgag ggcgttcatc ttttcagagt gaagcgttgc
    17281 cttccatgat tggtagatac caagtggcaa gataatcaag cgcacgataa tggttacgat
    17341 gatgatagcg acaccaaagc ctagaccttt atcagtagcg aagtacttga tagcttcagc
    17401 cataggggct ccgatggtat tccaaataaa tcctgttggc tgacctgtgg ttttatcgac
    17461 attgacacaa ccagtcaaga caagcaacat agccgctccc atagctgaga gtgcaaaacg
    17521 tttaatagat ttcactgttt ttattccttc tttaaaaatt atacctttct attctactgt
    17581 ttttttacaa aatatacaat agttctggag acctaatttg cgattttaaa gtcagagtag
    17641 ggaggaaagt tgcttagttt gacatctaag tagcttactt ttgaaaaagg tgtcggtcct
    17701 tttcggattt cttggataaa ttttgccatg atagcagatg agtctgcttg ggctaagatt
    17761 tccactgtgc catcgtcgtt attccatact cgacctgtga tgccaccaat ttcaagtgcc
    17821 aagctgtaaa caccccaacg aaagccgact ccctgtaccc taccttgggc aatcattcta
    17881 accttttgca taccaaaccc ctttgattgt gttataatat ttctatgact attataacct
    17941 caaaagccaa ttctgtggta aaaaatgcca agaaattacg ccaaaaaaaa taccgcaagt
    18001 ctgcctactt gattgaaggc tggcacttgt ttgaagaagc tgtccaagct ggagtgacga
    18061 ttgagaaaat ctttgcccta gaaaattatc gagatcagtt agctactttt tcgcaaactg
    18121 tctgggtgtc agaggatatt ttgctagatt tggcggattc tcagactcca cagggaattg
    18181 ttgccgtggt tcaaaaagaa gaagtaggac aagctgattt gagtcagggc aagttcttgt
    18241 ttttggaaga tgtgcaagat cctggtaatg taggaactat cattcgaact gcggatgcag
    18301 caggttttac aggagtgatt gtttcagata agtcggcaga catctacagt ctcaaaaccc
    18361 tacgttccat gcaaggtagt catttccatc tgcccattta ccggatgact agtcaagcgc
    18421 ttcttgacga aactaaaaag gtagctatcc cagtgctagc aacaacccta tctaaagatt
    18481 ctgttgatta cagagaactg ccttctatag aaaattttgt actagttatg ggaaatgagg
    18541 gtcaaggaat tagtcccctt atggctgaaa gtgcagacca gttggtccat attagcatga
    18601 aggggcaggc cgagagtttg aatgttgcgg ttgcagccgg tattttaatc ttccatttaa
    18661 gctaatttta actttttttg ttataatcaa ggaaagatgt tcacagaaaa ggagaaatgg
    18721 atgaatcaca ctattataca tgaccgtgca ggtctcaatc aattttacgc taaggtttat
    18781 gcctttgttg gtctgggaat cggactatct gctttggtat caggccttat gttgacggtc
    18841 tttcagtctc agttggttta ctttttgatg caggggcgtc tctggttgac cattgctact
    18901 tttgcggagt tggctttggt tttcgttgcc agtagcatgg cttcaagaaa tagtccagct
    18961 gctcttccag tatttttact ttactccgtt ttaaacggct ttacccttag ttttgtggtg
    19021 gctttctata ctccgggtac agttttatct gcctttgtat cgagcgccct tctcttcttt
    19081 gtcatggcgg cagttggtat ttttaccaag aaagacttga gtgggattgg tcgagcaatg
    19141 atggcagctc tcattggttt actgattgct atggtagtca atattttctt ggctagtggc
    19201 ttctttgatt atatgattag cgtagccatg gttttggttt tttcagggtt gattgcttgg
    19261 gataaccaaa agattcgcct cgcctatgaa caatcacaag gtcgtgtagc gacaggctgg
    19321 gtggtgtcaa tggctctcag catctatctt gattttatca atcttttcct aagtattcta
    19381 cgtatctttg gtcgcaatga ctaaaaggtc tatgaaatca gtgttttgag tgacactgat
    19441 tttttttaaa attttctctt ttcttttctt ggaaataggt gtataatacg tttattaaat
    19501 ttttgaggag ttgtctatga agaaaagttt tatccatcaa caagaagaaa tttcctttgt
    19561 caaaaacact tttacccagt atttgaaaga taagctagaa gttgtcgaag ttcaaggtcc
    19621 tatcttgagt aaggtcggtg acggaatgca ggacaacctg tctggtgtgg aaaatccagt
    19681 atcggtcaag gttctccaaa tccctgatgc tacttatgaa gtggtgcact cacttgctaa
    19741 atggaaacgc cacaccttgg ctcgttttgg ctttggtgaa ggagagggtc tctttgtcca
    19801 catgaaagcc cttcgtccag atgaggattc cttggatgca acccactctg tttatgttga
    19861 ccagtgggac tgggagaagg ttatcccaaa tggtaagcgt aacatcgttt atctaaaaga
    19921 aacagttgag aagatttata aggctattcg cctgactgag ctagctgttg aagcccgcta
    19981 tgacatcgag tctatcttgc caaaacaaat cacctttatc catacagaag aattggtaga
    20041 acgctaccca gacttgacac cgaaagaacg tgaaaatgcg atttgtaaag aatttggagc
    20101 cgtctttttg attggtatcg gtggcgagtt gccagatggt aaaccgcacg atggacgtgc
    20161 accagactat gatgactgga caagcgagtc tgagaatggc tacaagggtc taaatggtga
    20221 tattcttgtc tggaatgagt ctttaggtgg agcctttgag ttgtcttcta tgggaattcg
    20281 tgtagatgaa gaaactctta gacgtcaggt ggaaattaca ggtgatgaag accgcttgga
    20341 attggaatgg cacaagtctt tgttgaatgg tctattccca ttgacaatcg gtggaggaat
    20401 tggacaatct cgtatggcca tgttcctact tcgcaagaga cacatcggag aagtgcaaac
    20461 aagtgtttgg cctcaagaag tccgcgatac ttacgaaaat attttgtaga gaatcgaacc
    20521 gcaaggttcg gttttctttc tctttttgtc tataatttgg tataataaac agtatgaaaa
    20581 tcgtatcagg aatctatggg ggacgtcccc tcaagacact agaaggcaag acgacaagac
    20641 ctacttcgga taaggttagg ggagccattt ttaacatgat tggtccctac tttgaagtgg
    20701 gacgagtctt ggacctttat gcaggtagtg gtggtttatc tatcgaagca gtatcgcgtg
    20761 gcatgtccag tgctgttttg gtggagcgag accgtaaggc tcagaccatc gtggctgaaa
    20821 atatccagat gaccaaggaa gttggaaaat ttcaactcct aaagatggat gcagaaaggg
    20881 cattggaaca ggtatctggg gaatttgacc tcgttttctt agaccctccc tatgccaagg
    20941 aacaaatcgt agcagatatt gaaaaaatgg ctgagagaga gcttttttct gaagatgtta
    21001 tggttgtgtg cgagacggat aaagccgttg aacttccaga agaaattgcc tgtctgggta
    21061 tctggaagga aaagatttat ggaattagta aggtgacagt ctatgtcaga taagattggc
    21121 ttattcacag gctcatttga tccgatgaca aatgggcatc tggatatcat tgaacgggcg
    21181 agcagacttt ttgataagct ttatgtgggt atttttttta atccccacaa acaaggattt
    21241 ctccctcttg aaaatcgtaa acgggggcta gaaaaggctt tgggacattt ggaaaatgtt
    21301 gaagtcgtgg cttctcatga tgaattggtg gtcgatgttg caaaaagatt gggtgctact
    21361 tgtctagtgc gtggtttgag gaatgcgtcg gatttgcaat atgaagccag ttttgattac
    21421 tacaatcatc agctgtcttc tgatatagag actatttatt tacatagtcg acctgaacat
    21481 ctctatatca gttcatcagg cgttagagag cttttgaagt ttggtcggga tattgcctgc
    21541 tatgttcccg agagtatttt ggaggaaata agaaatgaaa aaaaagatta gatggccctt
    21601 atacgtcatt gcggccttga ttgtgacttt cttggcattt gtagtgccct tgccttatta
    21661 tatagaggtt ccaggtggtt cggaagatat tcgccaagtc cttaaaataa atgacacaga
    21721 agataaggaa gctggtgcct atcaattcgt tacggttggt gttcaacatg ccactttagc
    21781 tcatatgatt tatgcttggt tgacaccttt tacagatatt cgtagtgctc aggagactac
    21841 aggtggttct tccgatgttg aatttatgcg aatcaatcaa ttctacatgc aaacatcgca
    21901 aaatatggcc aagtatcaag gactaaaaac agctggtaag gatatcgaac tcaagtactt
    21961 tggagtttat gttttgaatg tgacggataa ttcaaccttt aaagggattc tcaatatctc
    22021 tgatacagtc acagcagtca atgatcagac ctttgatagt tccaaagact tgattgatta
    22081 cgtcagttct caaaaattag gggattccgt caaggtcacc tatgaagagg atgggcaaac
    22141 caagtctgca gaaggaaaga ttatcacctt ggaaaatggc aaaaatggaa ttggaatcgg
    22201 cttgattgac cgtacagagg taatcagcaa tgtcccaatt agcttttcaa cagctggtat
    22261 tggcggtcca agtgctggtc tcatgtttag tctagctatc tatactcaaa tagctcaccc
    22321 agatcttcgt aatggtcgta ttgttgccgg tacaggtacc attgaccgcg atggtaatgt
    22381 gggagacatt ggaggtattg ataagaaggt tgtagcttcg gctagggcag gtgctgctat
    22441 tttctttgct cctgataacc ctgttagcga agaagaacaa aaggcgcatc cggacgcgaa
    22501 aaacaactac caaacagccc tagaagcggc taaaacaatc aagacggata tgaaaatcgt
    22561 gcccgttaaa accctacaag atgcgattga ttacttgaaa aacaatccct aatttttgag
    22621 tgaagtttcc aaactagcgc acaaacagag aagatggtat aatagtcaaa tggttcaatt
    22681 attattcact ctaagtagtc atatgctctt tatttatgtg agtttttacc ttttaaagga
    22741 tcttgttaga tgggagaagg ttttaaaagt gacagatgag aatacaagaa aagttcgttt
    22801 attagtagcc ttttttagca ttgtcatagg ctacatcctg agttctttct ttattagcct
    22861 gtatcatttg tggcaagaag cgcttagagg attattatga aatcaagagt aaaggaaacg
    22921 agtatggata aaattgtggt tcaaggtggc gataatcgtc tggtaggaag cgtgacgatc
    22981 gagggagcaa aaaatgcagt cttacccttg ttggcagcga ctattctagc aagtgaagga
    23041 aagaccgtct tgcagaatgt tccgattttg tcggatgtct ttattatgaa tcaggtagtt
    23101 ggtggtttga atgccaaggt tgactttgat gaggaagctc atcttgtcaa ggtggatgct
    23161 actggcgaca tcactgagga agccccttac aagtatgtca gcaagatgcg cgcctccatc
    23221 gttgtattag ggccaatcct tgcccgtgtg ggtcatgcca aggtatccat gccaggtggt
    23281 tgtacgattg gtagccgtcc tattgatctt catttgaaag gtctggaagc tatgggggtt
    23341 aagattagtc agacagctgg ttacatcgaa gccaaggcag aacgcttgca tggcgctcat
    23401 atctatatgg actttccaag tgttggtgca acgcagaact tgatgatggc agcgactctg
    23461 gctgatgggg tgacagtgat tgagaatgct gcgcgtgagc ctgagattgt tgacttagcc
    23521 attctcctta atgaaatggg agccaaggtc aaaggtgctg gtacagagac tataaccatt
    23581 actggtgttg agaaacttca tggtacgact cacaatgtag tccaagaccg tatcgaagca
    23641 ggaaccttta tggtagctgc tgccatgact ggtggtgatg tcttgattcg agacgctgtc
    23701 tgggagcaca accgtccctt gattgccaag ttacttgaaa tgggtgttga agtaattgaa
    23761 gaagacgaag gaattcgtgt tcgttctcaa ctagaaaatc taaaagctgt tcatgtgaaa
    23821 accttgcccc acccaggatt tccaacagat atgcaggctc aatttacagc cttgatgaca
    23881 gttgcaaaag gcgaatcaac catggtggag acagttttcg aaaatcgttt ccaacaccta
    23941 gaagagatgc gccgcatggg cttgcattct gagattatcc gtgatacagc tcgtattgtt
    24001 ggtggacagc ctttgcaggg agcagaagtt ctttcaactg accttcgtgc cagtgcagcc
    24061 ttgattttga caggtttggt agcacaggga gaaactgtgg tcggtaaatt ggttcacttg
    24121 gatagaggtt actacggttt ccatgagaag ttggcgcagc taggtgctaa gattcagcgg
    24181 attgaggcaa gtgatgaaga tgaataagaa atcaagctac gtagtcaagc gtttactttt
    24241 agtcatcata gtactgattt taggtactct ggctctagga atcggtttaa tggtaggtta
    24301 tggaatcttg ggcaagggtc aagatccatg ggctatcctg tctccagcaa aatggcagga
    24361 attgattcat aaatttacag gaaattaggc tggagaacca gcctttttct aaagataagg
    24421 agaaatatga acaaaaaaac aagacagaca ctaatcggac tgctagtgtt attgcttttg
    24481 tctacaggga gctattatat caagcagatg ccgtcggcac ctaatagtcc caaaaccaat
    24541 cttagtcaga aaaaacaagc gtctgaagct cctagtcaag cattggcaga gagtgtctta
    24601 acagacgcag tcaagagtca aataaagggg agtctggagt ggaatggctc aggtgctttt
    24661 atcgtcaatg gtaataaaac aaatctagat gccaaggttt caagtaagcc ctacgctgac
    24721 aataaaacaa agacagtggg caaggaaact gttccaaccg tagctaatgc cctcttgtct
    24781 aaggccactc gtcagtacaa gaatcgtaaa gaaactggga atggttcaac ttcttggact
    24841 cctccaggtt ggcatcaggt caagaatcta aagggctctt atacccatgc agtcgataga
    24901 ggtcatttgt taggctatgc cttaatcggt ggtttggatg gttttgatgc ctcaacaagc
    24961 aatcctaaaa acattgctgt tcagacagcc tgggcaaatc aggcacaagc cgagtattcg
    25021 actggtcaaa actactatga aagcaaggtg cgtaaagcct tggaccaaaa caagcgtgtc
    25081 cgttaccgtg taacccttta ctacgcttca aacgaggatt tagttccctc agcttcacag
    25141 attgaagcca agtcttcgga tggagaattg gaattcaatg ttctagttcc caatgttcaa
    25201 aagggacttc aactggatta ccgaactgga gaagtaactg taactcagta aaagataagc
    25261 ctaaactcct atgtcactta tggatgtagg agttcttttt actagtttaa gcagggctag
    25321 aacaggtact aagaaaaaat agcaacttct aaaactaact tccagttttg ggagagagat
    25381 ggaagttact ttgagaagtt acggtaagaa aaaataatat gtattggatt aaattttgtg
    25441 atataataaa ggataagata ctattttagg agaagaacta tggaagaccc aagtagtcag
    25501 aatttgttgc tacaatttgt tttattgttt atcttgacgg tattaaatgc ctttttctca
    25561 gccacagaaa tggccatggt ttctcttaat cgtgcccgag ttgaacaaaa ggcggaagaa
    25621 ggagacagac gctacatccg cctgctgaag gtactagaaa atcctaacca ctttttatca
    25681 accattcagg taggaatcac cctgattaca atcttatcag gggcgagttt aactaatact
    25741 ctaggacgtg tgattgcctc ttggcttggg aatggcgaaa cagcacaagc cgtggcaact
    25801 tttctatcct tggcattttt gacctatatt tccatcgttt ttggggaatt atatcctaag
    25861 agaatcgctc ttaatctaaa ggatgccttg gcaattcgta tagcgccggt tatcattggg
    25921 atcggaaaac tagtcagtcc ttttgtttgg cttttagctg cttcgaccaa tttcttgagc
    25981 catttgactc ctatgtcatt cgatgatgcg gatgaaaaga tgacccgtga tgaaattgcc
    26041 tatatgctga caaatagtga agaaacattg gatgctgatg agattgagat gctacaaggt
    26101 gtcttttcgc tcgatgaact gatggcacga gaggttatgg ttcctcgaac ggatgccttt
    26161 atggtggata ttcaggatga tagtcaagcc attatccaaa gtattttaaa acaaaattat
    26221 tctcgtatcc cggtttatga tggggataag gacaatgtaa ttggaatcat tcacaccaag
    26281 agtctcctta aggcaggctt tgtggacggt tttgacaata ttgtttggaa gagaattttg
    26341 caagatccac tttttgtacc tgaaactatt tttgtggatg acttgctaaa agaactgcga
    26401 aatacccaaa gacaaatggc aatcttgctg gatgaatacg gtggtatggc tggtttggtg
    26461 actttggaag acctcttaga ggaaattgtt ggtgaaattg atgatgaaac ggacaaagca
    26521 gcaatagatg ttcatcagat tggcgaggat acctacattg ttcaaggaac tatgaccctc
    26581 aatgatttca ataattactt tgatgttgaa ctggaaagtg atgacgttga taccatcgct
    26641 ggttattatt tgacaggagt gggaactatt ccaacgactg agaaactcag ctatgaattg
    26701 gttagccaaa acaaacagtt tatcttaacc aatgataaag tgaagaatgg acgtgttacc
    26761 aaggtaaaag tccaaatcac agaagtcgaa atagaagaag aaacagaata aactagaggc
    26821 ttttgtcacc aggcgtgacg aagctttttt caattgattt caaaagggaa ttatgccatg
    26881 gaaagaactc tgccacaaac tcgctccaaa agtttttaaa gttatacgta tttattcgag
    26941 ggaaaataaa aaaagccctt caaattgggc tttctgttca tttgaaacct gataaatcaa
    27001 gttatagaag gcggtagacg gatttgaacc gacgatcaag cttttgcaga gccgtgcctt
    27061 accacttggc tataccgcct taacgtttat tattatacct tgaaaatgat tttcagtcaa
    27121 tagttttttc agttttttgt accatagcgt gattcgaatg tgatgccaag ttttctatgg
    27181 gaaaaaattt accaaaaacc tactaaaacc ccttgaaaaa tctagctaaa tagggtataa
    27241 tatgggtaat catttgtcgt aggttttgtc tgaaatattg tccagacaag gctcacagca
    27301 gttaaatctt ctgaaaaagt cagatttagc tgctcttttt gtgctttttt tcaagatttt
    27361 gagcatttgt aacagaggct taaagattct gaaaattcgt caagaggaca cggtgataag
    27421 gggtttacaa ccatatgacg attagaaaag cctgattgac aaggcttgga acttatttac
    27481 aaaggagaat catcttggca ggacatgacg ttcaatacgg gaaacatcgt acccgtcgta
    27541 gtttttcaag aatcaaagaa gttcttgact taccaaattt gattgaaatt caaactgact
    27601 cattcaaagc tttcctagac cacggtctta aggaagtgtt tgaagatgta ttgccaattt
    27661 caaacttcac agacacaatg gagttggaat ttgttggata tgaaatcaag gaaccaaaat
    27721 atacgctaga agaagctcgt atccacgatg ctagctactc agcaccaatt tttgtaacct
    27781 tccgtttgat caataaagaa acaagcgaaa tcaagaccca agaagttttc tttggtgatt
    27841 tcccaatcat gacagaaatg ggtactttca tcatcaatgg tggtgaacgt attatcgttt
    27901 ctcagttggt ccgctcacca ggtgtttact ttaacgacaa agtagacaaa aatggtaagg
    27961 tgggctatgg ttcaactgtt atccctaacc gtggagcttg gttggaactt gaaagcgact
    28021 caaaagatat cacctacact cgtatcgacc gtactcgtaa gattccattt acaaccttgg
    28081 ttcgtgctct tggtttctca ggtgatgatg aaatctttga tatctttggt gacagcgaat
    28141 tggttcgcaa cactgttgaa aaagatatcc acaagaatcc aatggactct cgtacagacg
    28201 aagccttgaa agaaatttac gaacgccttc gtccaggtga gcctaagacg gctgaaagct
    28261 cacgtagctt gcttgtagct cgcttctttg acccacgtcg ttatgacttg gcagcagttg
    28321 gtcgttacaa aatcaataaa aaactcaatg ttaaaacacg tttactcaac caaaccattg
    28381 cagagccatt ggtagaccct gaaactggag aaatcttggt agaagctggt acgattatga
    28441 ctcgtagcgt gattgaaagc attgaaagcc atttggatgg cgacttgaac aagattgtct
    28501 acatcccaaa cgatgcagcc gttgtgactg agcctgttgt tcttcaaaaa ttcaaggtta
    28561 ttgctccaac tgatccagat cgcgtcgtaa cgatcattgg taatgctaac ccagatgaca
    28621 aggttcgtac ggtgactcct gcagatatcc ttgctgagat gagctacttc ctcaacttgg
    28681 ctgaaggact tggccgtgta gatgatatcg accaccttgg aaatcgtcgt atccgtgcgg
    28741 ttggtgaatt gcttgccaac caagtacgtt tgggactttc tcgtatggaa cgtaatgtcc
    28801 gtgaacgtat gtctgttcag gacaatgaag tcttgacacc acaacaaatt atcaatattc
    28861 gtcctgtaac agctgcagtt aaagaattct ttggttcatc acagttgtca cagttcatgg
    28921 accaacacaa cccgctttct gagttgtctc acaaacgccg tttgtcagcc ttaggacctg
    28981 gtggtttgac tcgtgaccgt gctggatatg aagtgcgtga cgtgcactac actcactatg
    29041 gtcgtatgtg tccaatcgag acacctgaag gacctaacat cggtttgatc aataacttgt
    29101 catcttacgg acacttgaac aaatatggtt ttgttcaaac accataccgt aaggttgacc
    29161 gtgaaacagg tgttgtcacg aacgaaattg tttggttgac agctgatgaa gaagatgaat
    29221 atactgtagc tcaggctaac tctcgtctga atgaagatgg aacctttgct gagaagattg
    29281 tcatgggacg tcaccaaggg gtcaaccaag agtatccagc taatattgtt gactacatgg
    29341 acgtttcacc aaaacaggta gttgccgttg cgacagcatg tattcctttc ttggaaaacg
    29401 atgactccaa ccgtgccctc atgggagcca atatgcaacg tcaggctgtg ccactgatta
    29461 atcctcaggc accttacgtt ggtactggta tggaatacca agcagcccac gattctggtg
    29521 cggctgtgat tgctcagtat gatggtaaag ttacttacgc agatgctgac aaggtagaag
    29581 ttcgtcgtga agatggttca ttggatgttt accacatcca aaaattccgt cgttcaaact
    29641 caggtactgc ttacaaccaa cgcactctcg taaaagttgg tgatgtcgtt gaaaaaggcg
    29701 atttcatcgc tgacggacct tctatggaaa atggagaaat ggcgcttgga caaaacccaa
    29761 tcgttgccta catgacttgg gaaggttaca acttcgagga tgccgttatc atgagcgaac
    29821 gcttggtgaa ggacgatgtc tacacatctg ttcaccttga agaatacgaa tcagaaacgc
    29881 gcgatacaaa gcttgggcct gaagaaatca ctcgcgaaat tccaaacgtt ggtgaagatg
    29941 ccctcaaaga ccttgacgaa atggggatta tccgtattgg tgctgaggtt aaagaaggtg
    30001 atattcttgt aggtaaagta acacctaagg gtgagaaaga tctttcagct gaagaacgtc
    30061 tcttgcacgc tatctttgga gacaagtctc gtgaagtgcg tgatacttct cttcgtgtac
    30121 cacacggtgc cgatggtgtc gttcgtgatg ttaagatctt tacacgtgta aatggagatg
    30181 agttgcaatc aggtgttaac atgttggttc gtgtttacat cgctcaaaaa cgtaagatta
    30241 aggtcggaga taaaatggcc ggacgtcacg gaaacaaagg ggttgtctct cgtatcgttc
    30301 ctgtagaaga catgccttac cttccagacg gaactccagt cgacatcatg ttgaacccac
    30361 ttggggtacc atcacgtatg aatatcggtc aggttatgga gcttcacctt ggtatggcag
    30421 ctcgtactct tggtattcac atcgctacac cagtctttga tggagcaagt tctgaagatc
    30481 tttggtcaac tgttaaagaa gcaggtatgg atagcgatgc caaaacgatc ctttacgatg
    30541 gacgtactgg tgaaccgttt gataaccgtg tttctgtcgg agtcatgtac atgatcaaac
    30601 tccaccacat ggttgatgat aaattgcacg cgcgttcagt cggaccatac tcaactgtta
    30661 cccaacaacc actcggaggt aaggctcagt ttggtggaca acgtttcggt gagatggagg
    30721 tttgggctct tgaagcctac ggtgcgtcaa atgtccttca agaaatcttg acttacaagt
    30781 cggacgatat caacggacgt ttgaaagcct atgaagctat tacaaaaggc aaaccaattc
    30841 caaaaccagg tgttccagaa tccttccgag ttcttgtcaa agaattgcaa tctcttggtc
    30901 ttgacatgcg tgtcctagac gaagatgacc aagaagtgga acttcgcgac ttggatgaag
    30961 gaatggacga agatgtcatc cacgtagatg accttgaaaa agcccgcgaa aaagcagccc
    31021 aagaggctaa agcagccttt gaagctgaag aagctgagaa agcaacaaaa gcggaagcaa
    31081 cagaagaagc tgctgaacaa gaataagcag ttcacttaga atagaaaggg aagaaatagt
    31141 ggttgatgta aatcgtttta aaagtatgca aatcacccta gcttctccaa gtaaagtccg
    31201 ttcatggtct tatggagaag tcaaaaaacc tgaaacaatc aattatcgta ccttgaaacc
    31261 agaacgtgaa ggactctttg atgaagtgat ctttggtcct acaaaagact gggaatgtgc
    31321 ttgtggtaag tacaaacgca ttcgttacag aggaattgtt tgtgaccgct gtggggttga
    31381 agtaacgcgt acgaaagttc gtcgtgagcg tatgggacat atcgaattga aagctcctgt
    31441 atctcacatc tggtacttca aggggattcc aagccgtatg ggcttgaccc ttgatatgag
    31501 ccctcgtgcc ctcgaggaag ttatctactt tgcggcttat gtggtgattg atcctaagga
    31561 tacaccactt gagcacaagt ctatcatgac agagcgcgaa taccgagagc gcttgcgcga
    31621 atatggttat ggttcatttg ttgctaagat gggtgcggaa gccatccaag accttttgaa
    31681 gcaagtagat cttgaaaaag aaattgctga actcaaagaa gaattgaaaa ctgctactgg
    31741 acaaaaacgt gtcaaagcca tccgtcgttt ggatgttttg gatgcctttt acaagtctgg
    31801 aaacaaacct gaatggatga ttcttaacat ccttccggtt atcccaccag atcttcgtcc
    31861 aatgttgcag ttggatggtg gccgttttgc ctcatctgac ttgaatgacc tttaccgccg
    31921 tgttatcaac cgtaacaacc gtttggctcg tttgcttgag ttaaatgcac caggtatcat
    31981 cgttcaaaat gagaagcgta tgcttcaaga agcagttgac gctttgattg acaatggtcg
    32041 tcgtggtcgt ccaatcacag gaccaggtag ccgtccattg aaatcattga gccacatgct
    32101 taaaggtaaa caaggacgct tccgtcaaaa cttgctcggt aaacgtgttg acttctcagg
    32161 acgttccgtt atcgccgttg gtccaactct taagatgtac caatgtggtg tgccacgtga
    32221 aatggcgatt gaactcttta aaccatttgt catgcgtgaa atcgttgccc gtgatatcgt
    32281 gcaaaacgtc aaagcagcta aacgcttggt ggaacgcgga gatgagcgta tctgggatat
    32341 ccttgaagaa gtgattaaag aacacccagt gcttttgaac cgcgcaccga cccttcaccg
    32401 tttgggtatc caagccttcg agccagtctt gattgatggt aaggctcttc gcttgcaccc
    32461 acttgtctgt gaagcctaca atgctgactt tgacggggac caaatggcca tccacgtacc
    32521 actttcagaa gaagcacaag cagaagctcg tatcctcatg ctagctgctg agcacatctt
    32581 gaacccgaaa gatgggaaac cggtagttac tccatctcag gacatggttt tgggtaacta
    32641 ctacttgacc atggaagaag ctggtcgcga aggtgaagga atggtcttca aagaccgtga
    32701 cgaagcggtt atggcttacc gcaatggtta tgttcacctc cactcacgtg ttggtatcgc
    32761 aacagacagc ctcaacaagc cttggacaga agagcaaaga cataaggtct tgcttacaac
    32821 agttggtaaa attctcttca acgatatcat gccagagggg ctaccatact tgcaagaacc
    32881 aaacaatgcc aacttgacag aaggtgttcc agctaaatac ttcttgccac ttggtggagc
    32941 tatcaaggaa gctatcagca atcttgagct caaccctcca ttcaagaaga aaaacctcgg
    33001 aaatatcatc gctgaaatct tcaaacgttt ccgtacgaca gaaacttctg ccctacttga
    33061 ccgcatgaag aacctcggtt accaccactc aactcttgca ggattgacag tgggtattgc
    33121 cgatatccca gtcgttgatg acaaggctga aatcattgaa gaatcacaca aacgtgtcga
    33181 acaaatcacc aaacaattcc gtcgtggtat gatcacagac gacgagcgtt acaatgctgt
    33241 tacagctgaa tggcgtgctg cccgtgaaaa acttgagaaa cgcttgattg ctaatcaaga
    33301 tcctaagaac ccaatcgtta tgatgatgga ctctggagcc cgtggtaaca tctcaaactt
    33361 ctcacagctt gccggtatgc gtggtctgat ggctgctccg aatggacgta tcatggaatt
    33421 gccaatcctt tcaaacttcc gcgaaggttt gtcggtattg gaaatgttct tctcaactca
    33481 cggtgctcgt aaaggtatga ccgatacggc ccttaagaca gccgactcag gttacttgac
    33541 tcgtcgtttg gtcgacgttg cccaagacgt tatcatccgt gaggacgact gtggaactga
    33601 ccgtggtctc ttgatccgtt ctatcgcaga aggaaaagag atgatcgagt ctctcgaaga
    33661 gcgtctcaac ggtcgttaca ctaagaaaac tgttaaacat ccagaaactg gtgcagtgat
    33721 tattggtcca aatgaattga ttacagaaga caaggcgcgt gaaattgtca atgctggtgt
    33781 ggaagaagtg actatccgtt ctgtatttac atgtaacact cgtcacggtg tctgccgtca
    33841 ctgttacggt atcaacttgg cgactggtga tgcggttgaa gttggtgaag cagttggtac
    33901 aatcgctgcc caatctatcg gggaacctgg tacacagctt acaatgcgta ccttccacac
    33961 aggtggggtt gcctcaaata ccgatatcac tcagggtctt cctcgtgtcc aagaaatctt
    34021 tgaagcccgc aatcctaaag gggaagcggt tattacagag gttaaaggac aagttactgc
    34081 tatcgaagaa gatgcatcaa ctcgtaccaa gaaagtcttt gttaagggtg aaactggcga
    34141 aggtgaatat gtcgttccat ttacagctcg tatgcgtgtc gaagttgggg gccaagtagc
    34201 gcgtggtgct gctctgacag aaggttctat ccaaccaaaa cgtctccttg cagttcgtga
    34261 tgtcttgtca gttgaaacgt accttctcgg tgaagtacaa aaagtttacc gtagccaagg
    34321 ggtagaaatc ggtgacaaac acatcgaggt aatggttcgt caaatgatcc gtaaagtccg
    34381 tgtcatggat ccaggtgata cagatcttct catgggtacc ctcatggata tcaatgactt
    34441 tacagatgcc aacaaagatg tccttatcgc aggtggagtt ccagcgacag gtcgcccagt
    34501 ccttatggga attaccaaag cctcacttga aacaaacagt ttcttgtcag cggcttcctt
    34561 ccaggaaaca actcgtgtcc ttactgacgc agctatccgt ggtaagaaag accatctcct
    34621 tggacttaaa gaaaatgtta tcatcggtaa gatcatccca gctggtactg gtatggcccg
    34681 ttaccgtaac cttgaaccac atgctgtcaa cgaagaagaa taccttaatc ctccagtaga
    34741 ggaagaagga aatgaagaaa caacagaagt agttgtggat actgccgttg aaactgtgga
    34801 agaaacagta gaataagaga gaatgagaga acccgagggt tctctcattc tctcttctga
    34861 aaactttctc catttttcca cgaaatgtgg taaaataaaa gaaagaagct gacaaaggag
    34921 acgggtatgg aacaaacatt ctttatcatc aaaccagatg gtgtaaaaag agggctagtg
    34981 ggtgaagtgt taaagcgcat cgaacaacgt ggatttacaa tcgaaaaatt ggagtttcgt
    35041 tcacaggttt cagaagagtt gattgaccag cactatcagg acttggttgg tcagagtttt
    35101 tacccaccga ttcgtgaatt catgacttca ggtccagttc ttgtgggtgt catttctggt
    35161 cccaaagtaa tcgaaacttg gcggaccatg atgggtgcaa ctcgtccaga agaagcttta
    35221 ccaggcacta ttcgaggtga ttttgcaaaa gctgctggag aaaatgagat tatccaaaat
    35281 gttgtacatg gttcagattc agaagagtca gctaagcgag aaattgctct ttggttttaa
    35341 gagtggattg gctcaatcaa ttggttaaaa gctcatttga atagaaagta tagtcaatta
    35401 gtttaagaca tgacgcatga tatcaaactt tttagttttt gatatggtgc gttttttgat
    35461 taagtgacta ttagtttgtc tcgctccgtt aggtgcgaga caaaaaaaac acccgctatg
    35521 cgggtgtgcg tcgaaggtta taccaaaaaa actccaaacg cgatacaata aaggtgttca
    35581 atccaattgt aaagcgaaag gagaaaaata tggcacaaaa ggttctttta tcctgttctt
    35641 gtttcaattg actatatttc tatacttttt cttcctttct ctgtgaaaat cgcaagtttt
    35701 tcccaccttt tctcaatact gaaacaacat ctttccagaa cttcaataat ttctttttgt
    35761 gttaaatcat tatttaacta aatctttgga ggcaatacgt tttcaaagct acttttttac
    35821 cataggtttt gagccttcaa cacagattac taagccaaaa atgctctaaa tttttgaaat
    35881 tctaacaaat tccactcaac tttatcatca tctttgactt tcacaatatc ttcctcaaaa
    35941 taacatggaa taaagatagt tttcttttaa aacggttgta ggaggaaaat ctctcctaca
    36001 ctattattga ttctgagaga aaattgcaaa atatcatcta ccaaactttc aattattaag
    36061 attttttgca gtttatctga attagaatcg caaggcttct caataatata attgtcgaat
    36121 tttttcattc taacttcctc tccattattt cttcctttat caagaaaaag ttacctcaat
    36181 attaataaag tacaattcta aattaactcc aattcttgat tcatgataat atttcagtaa
    36241 ctcatcgtgc aattaaaaac aaggattttt atttcagatg atccatcgtg aacacttcat
    36301 cagccatctc ccaaattgcc ggattatgtg ttgcgataat gattagccta ttatcatctc
    36361 gaagagatag caaaatctcc ataatcaact gagaggttgc tgggtctatt gaagctgttg
    36421 gctcatctgc cagaataaag ggtggattct ttaagataat ttttgccaag gcaacccgtt
    36481 gcgattctcc gcccgataac tcaaagatgc gcttatctag gtcaagataa accaggccga
    36541 cctgttctaa agcctgcttc tgcctcaacc gctgttccga ccgactcaac ttttgaccaa
    36601 tgagacctag cttaaggttt tcttcaatac tttggttttc aattaagcca aagttctgga
    36661 agaggtagcc caattcgtga cggaaaaaat cacttgattt ataattggcc aagtctttac
    36721 ctcggtaaaa aatcgtccca tcataaggtt ctaatttccc aatcatgttc atcaaggttg
    36781 tttttccgct accacttgaa ccaattaagg catagacttt tccagcctca aatgtcattg
    36841 aaagattcga aaataactct cgttctccaa aagatttact cacctgttta agttcaatca
    36901 tattaacctc ccttcaaaac aagcatggac atcttgtttt ctttctgcat ttggacatgt
    36961 aactgtaaaa gagatagacc agtaaagagt aacaagacta agaaagcaac tcctatctct
    37021 acctgaagaa atacactcgc aacaaatccc agtaaaaaca cacccagttg agcaaagaga
    37081 taagtgcgat ggatttctaa gaacctgaga cctgcaatgc gtttgataaa aatggcacgt
    37141 ctaaattctt caaagtagag cctattcata gtgttaaaca acaagattga agttgcaatc
    37201 ccaagcacag ctcctgctag cattacccaa cgttccctct ggatattatc caataatgtg
    37261 atgtagttgt ggtaacctgt ttgcatttct gagacccaat tttcaatgcc ttgtctctgg
    37321 ataagctcct gggcatcaga caattgatta aagagaacgt agttctgtac tgcgtctatc
    37381 caaaacaaaa tggactgtgg accagttgat tggggtgtta taacaatgat gattggatct
    37441 ttcaaaaact gctggtaaga aataggggtc gtattataca caaaacgatc ctgacctgat
    37501 tctaaataac ctaccgtagc agtcatttgc tgtcgttcat cttgactaga tatgcgactg
    37561 gttaagtcgt cttcaaaaac agatttataa tgttcttcct ctgaacggag gtgttcaggc
    37621 agcaataaga caaactcccc tacatcaagg tgattcatct tttgactcat tttagttgaa
    37681 ctgtaggact tactttttca gagggctcca aattttattt tcatttactt tttttagccc
    37741 ataaaatatt taccatactt ataaaaagta gtaatactcc aactaaactg acttgtaatg
    37801 aattatatgt attttctgaa gtgttaggta aataattttg agagtaccct tctcgggcaa
    37861 tagtaataat attgt
//
LOCUS       DAVLVF010000012        37514 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVLVF010000012 DAVLVF010000000
VERSION     DAVLVF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.012"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(47..1714)
                     /locus_tag="VYN66_000564"
     CDS             complement(47..1714)
                     /locus_tag="VYN66_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV1494627.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(1717..2082)
                     /locus_tag="VYN66_000565"
     CDS             complement(1717..2082)
                     /locus_tag="VYN66_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1494628.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(2239..2427)
                     /locus_tag="VYN66_000566"
     CDS             complement(2239..2427)
                     /locus_tag="VYN66_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV1494629.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(2543..3028)
                     /locus_tag="VYN66_000567"
     CDS             complement(2543..3028)
                     /locus_tag="VYN66_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEV1494630.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNVQIDSSILKGYRLPFEGLSYAAFFRYFIPKYVSESRVL
                     YLDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENVTQDL
                     I"
     gene            complement(3297..4841)
                     /locus_tag="VYN66_000568"
     CDS             complement(3297..4841)
                     /locus_tag="VYN66_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV1494631.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            5054..5356
                     /gene="gatC"
                     /locus_tag="VYN66_000569"
     CDS             5054..5356
                     /gene="gatC"
                     /locus_tag="VYN66_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV1494632.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            5356..6822
                     /gene="gatA"
                     /locus_tag="VYN66_000570"
     CDS             5356..6822
                     /gene="gatA"
                     /locus_tag="VYN66_000570"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV1494633.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWNHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            6822..8264
                     /gene="gatB"
                     /locus_tag="VYN66_000571"
     CDS             6822..8264
                     /gene="gatB"
                     /locus_tag="VYN66_000571"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV1494634.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            8393..8953
                     /gene="efp"
                     /locus_tag="VYN66_000572"
     CDS             8393..8953
                     /gene="efp"
                     /locus_tag="VYN66_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV1494635.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            8975..9364
                     /locus_tag="VYN66_000573"
     CDS             8975..9364
                     /locus_tag="VYN66_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1494636.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            9357..9779
                     /gene="nusB"
                     /locus_tag="VYN66_000574"
     CDS             9357..9779
                     /gene="nusB"
                     /locus_tag="VYN66_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV1494637.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     AVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(9828..10055)
                     /locus_tag="VYN66_000575"
     CDS             complement(9828..10055)
                     /locus_tag="VYN66_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1494638.1"
                     /translation="MLSGLIFGLSHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRL
                     TILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(10546..10731)
                     /gene="briC"
                     /locus_tag="VYN66_000576"
     CDS             complement(10546..10731)
                     /gene="briC"
                     /locus_tag="VYN66_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV1494639.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(10890..11657)
                     /locus_tag="VYN66_000577"
     CDS             complement(10890..11657)
                     /locus_tag="VYN66_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV1494640.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(11654..12520)
                     /locus_tag="VYN66_000578"
     CDS             complement(11654..12520)
                     /locus_tag="VYN66_000578"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV1494641.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(12557..13924)
                     /gene="accC"
                     /locus_tag="VYN66_000579"
     CDS             complement(12557..13924)
                     /gene="accC"
                     /locus_tag="VYN66_000579"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV1494642.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASRNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(13936..14358)
                     /gene="fabZ"
                     /locus_tag="VYN66_000580"
     CDS             complement(13936..14358)
                     /gene="fabZ"
                     /locus_tag="VYN66_000580"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV1494643.1"
                     /translation="MIDIQGIKEAIPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(14355..14840)
                     /locus_tag="VYN66_000581"
     CDS             complement(14355..14840)
                     /locus_tag="VYN66_000581"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV1494644.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(14843..16078)
                     /gene="fabF"
                     /locus_tag="VYN66_000582"
     CDS             complement(14843..16078)
                     /gene="fabF"
                     /locus_tag="VYN66_000582"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV1494645.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(16100..16831)
                     /gene="fabG"
                     /locus_tag="VYN66_000583"
     CDS             complement(16100..16831)
                     /gene="fabG"
                     /locus_tag="VYN66_000583"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV1494646.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(16865..17785)
                     /gene="fabD"
                     /locus_tag="VYN66_000584"
     CDS             complement(16865..17785)
                     /gene="fabD"
                     /locus_tag="VYN66_000584"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV1494647.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTSLLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTTHLAHVEDQASLVALLKK"
     gene            complement(17778..18752)
                     /gene="fabK"
                     /locus_tag="VYN66_000585"
     CDS             complement(17778..18752)
                     /gene="fabK"
                     /locus_tag="VYN66_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV1494648.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(18871..19095)
                     /locus_tag="VYN66_000586"
     CDS             complement(18871..19095)
                     /locus_tag="VYN66_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1494649.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(19155..20129)
                     /locus_tag="VYN66_000587"
     CDS             complement(19155..20129)
                     /locus_tag="VYN66_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV1494650.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(20129..20563)
                     /locus_tag="VYN66_000588"
     CDS             complement(20129..20563)
                     /locus_tag="VYN66_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1494651.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(20657..21442)
                     /locus_tag="VYN66_000589"
     CDS             complement(20657..21442)
                     /locus_tag="VYN66_000589"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV1494652.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            21811..23175
                     /locus_tag="VYN66_000590"
     CDS             21811..23175
                     /locus_tag="VYN66_000590"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV1494653.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            23178..23549
                     /locus_tag="VYN66_000591"
     CDS             23178..23549
                     /locus_tag="VYN66_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV1494654.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            23763..25037
                     /gene="serS"
                     /locus_tag="VYN66_000592"
     CDS             23763..25037
                     /gene="serS"
                     /locus_tag="VYN66_000592"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV1494655.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVEAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(25062..25961)
                     /locus_tag="VYN66_000593"
     CDS             complement(25062..25961)
                     /locus_tag="VYN66_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV1494656.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACSGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(26071..26619)
                     /locus_tag="VYN66_000594"
     CDS             complement(26071..26619)
                     /locus_tag="VYN66_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV1494657.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(26837..28159)
                     /locus_tag="VYN66_000595"
     CDS             complement(26837..28159)
                     /locus_tag="VYN66_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV1494658.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFTFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(28250..28338)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(28502..30838)
                     /locus_tag="VYN66_000596"
     CDS             complement(28502..30838)
                     /locus_tag="VYN66_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV1494659.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKGQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQIGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(30942..31490)
                     /locus_tag="VYN66_000597"
     CDS             complement(30942..31490)
                     /locus_tag="VYN66_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV1494660.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(31487..31789)
                     /gene="zapA"
                     /locus_tag="VYN66_000598"
     CDS             complement(31487..31789)
                     /gene="zapA"
                     /locus_tag="VYN66_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV1494661.1"
                     /translation="MANLNRFKFTFGKKTLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            31876..32757
                     /locus_tag="VYN66_000599"
     CDS             31876..32757
                     /locus_tag="VYN66_000599"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV1494662.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            32761..33375
                     /gene="lepB"
                     /locus_tag="VYN66_000600"
     CDS             32761..33375
                     /gene="lepB"
                     /locus_tag="VYN66_000600"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1494663.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            33609..35975
                     /locus_tag="VYN66_000601"
     CDS             33609..35975
                     /locus_tag="VYN66_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV1494664.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKNNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNSIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(36023..37306)
                     /locus_tag="VYN66_000602"
     CDS             complement(36023..37306)
                     /locus_tag="VYN66_000602"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV1494665.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDQKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT        10877 a         8637 c         7264 g        10736 t
ORIGIN      
        1 tttactaaaa agaaaaaaga actgatttct cagtccttca ttaatcttat tccacactaa
       61 ataggtatgg gtaaacaggt tgttgacctt ggtgaatctc gacttcaacg tcttcgaatt
      121 cttctacgat ttcttgagcg atttcattgg caagttcttc gcttccgtct tcaccgacat
      181 agaaggttac gatttcactg tcttcatcca acatatgttt caaggtttca gtcaatgttt
      241 ggtgcatatc agggtttgac acaagaattt ttccatccac catacctaaa ttatcgtttt
      301 catggatttc taagccatcg atcgttgtat cacgcacggc tgttgtgacg cttccgctaa
      361 cgacatcgct aagagcagct gtcatacgct cttggttttc ttcaatggac ttgcttggat
      421 caaaggcaag aagacttgtc ataccttgag gaagagtgcg agcctctacc actaccgctg
      481 gttgctccaa aacttctgcc gcagattgag ctgccatgaa gatgttcttg ttgtttggca
      541 agaagatgat gttacgggca ttaacctgtt caacagcctt gataaagtct tctgttgaag
      601 ggttcatggt ttgaccgcct tcgataacat aatccacgcc ttgagaacag aagatatctg
      661 ctagaccttt accagccacc acagcaatca aagcatactc tttttcttca gccgacttga
      721 taacttgagt agcttctttc tcaacctgtg cttcgtgttg gttacgcata ttgtcaactt
      781 ttaccttgac caagctacca tatttgagac cttcttgcat aacaagtcct ggatcttctg
      841 tatgaacatg gactttgaca atttcatcat cgttaacaac gaggagagaa tctccaagct
      901 catccaagta gttacggaat tcatcgtagt caaaatcttt agcataggtt ggaccttgct
      961 taagagctac catgatttca gtacagtaac caaacgtgat gtcctcagtc gctacgtgac
     1021 cagctacaga cttatgatgc tctacattga tcatctcact catgttggca ggagtcgcta
     1081 caaagtcctc agatgcaata tattcgccag taagggctga aaggaaacct tcgtagatga
     1141 agaccaatcc ttgaccacct gagtccacaa cgccaacttc tttcaatact ggaagcatgt
     1201 ctggtgtttt agctagagct gttttagcac cttccaaggc tgcgcgcatg acttcaacag
     1261 tgtcatctgt ttgctcagct tttttcttag caccgatagc agctccacga gaaactgtca
     1321 aaatcgttcc ttcaacaggt ttcatcactg ccttataggc aacttccaca cctgattgga
     1381 aggccagagc caagtcttga cctgttaact cgtctttatc cttgatagct tgggaaaatc
     1441 cacggaaaag ctgagacgta atcactcctg agttcccacg cgcacccatc aaaagccctt
     1501 tggcaagaat gctcgctact tctccaactg tagaagctgg cttgtctgca acttctttag
     1561 caccattttc aatggtcatt cccatatttg tcccagtatc tccatctgga actggaaaga
     1621 cgtttaatga attgacatat tcagcttgct tattcaagcg agttgatgca gcctgcacca
     1681 tttcttgaaa taagctagta gtaatttttg acacggttat tctcctacaa ctttgatatt
     1741 ttgaatgtag acatttacag tctgagcagt aattccaagc tggttttcca agctaaaggc
     1801 aacacgctct tgaatgtttt ttgacacttc actaatcttt gttccgtagc ttaacacggt
     1861 atatacatca actgcaatac tgccatcttc ggctgccttt acgacgacac ctttagaata
     1921 attttcctta cctagcaggg cttggaaatt atctttgagg gcatttttac tagccatacc
     1981 gaccacacca aaaatctcag ttgctgctcc acctacgacg gttgcaatca cttcatctgt
     2041 tagttcgatt tgaccatctt ttgtattaat ttttacagtc atccttttta cctcaactag
     2101 ttgatactct attttatcat atttcagccc aagtgtaaaa gcagaatgat gtatcagcgg
     2161 atatttactc tatttttcaa acgatgttat acccacaata aaagaaaaaa gaccctaagg
     2221 tctcctttgc ttttattatt aaacgcgttc aactttacct gatttcaaag cacgagctga
     2281 agcccaaact tttttaggtt taccatcgat aagaacagta actttttgaa ggtttggttt
     2341 tacggcacgt tttgtttggt tcatcgcgtg tgaacggttg tttcctgata cagtcttacg
     2401 acctgtaaag taacatactt tagccattgt gttttcctcc tattagatct aatatagcgg
     2461 atgtgctagc accacatacc gtactatgtt atcacacttt cttgtttttt gcaagggaat
     2521 tggaagattt tttatttgtg tcttaaatca ggtcttgcgt gacattttct gctctccaca
     2581 tgccattgtt gattaacaga acaccagaat taaaattatg tgtataaaaa tcatctctaa
     2641 ctgcagctaa gggtatagcc gtcaagtcca aatcccacag ctcatctatc gattttctta
     2701 caacaatatc tgaatccaaa tacagtacac gagactcgct tacatacttt ggaataaaat
     2761 acctaaaaaa agccgcatat gaaagtccct caaaggggag acgataacct ttcagaatac
     2821 tactgtcaat ctgaacattc acaatctcac tattcaaagt ctctagtctt ttttccatca
     2881 attggaacca ttctcgcgga aggtcatcat taaaaacata aaacttaaga ttataatgat
     2941 gaacacaaag agattttatt gttgtttcaa ctttatccat ataagcatta tctgcaccta
     3001 agacaatcgc ttttttctct tctttcactc tttatcttat ttctttttat tcccatcata
     3061 ttattcccat catatgtttc tacgtaacca ttattttcgc ctattcgttc gtaaaaccat
     3121 accagtggag attttagatg aagtcccatt acggtttaca atttttacat tacgacacgg
     3181 agttttacaa atcgatttca tttgccaaac gtagttagtg aggcagttag ctagttcgcc
     3241 aaatagcgac tagcgtccaa caatttggaa ctttagctcc aattgttggt actgagtcac
     3301 atcttctcct ctaactctac gtctggatac ttgtccgcaa accagcggag ggcaaagtca
     3361 ttttcaaaga gaaagactgg ttggtcaaaa cggtctttgg ctaagatatt gcgacttgac
     3421 gacatccgtt catccaagtc ctcaggcttg atccaacgaa cggtcttttt acccattggg
     3481 ctcatgacca cttccgcatt gtattcgcct tccatacggt gtttaaagac ttcaaactgg
     3541 agttgaccaa cagctcccag catgtactca cctgtttggt aattcttata aagctgaacg
     3601 gctccttctt gcaccaattg ctcaatcccc ttgtggaagg atttttgctt cataacattc
     3661 ttggcagaaa ctttcatgaa aatttcagga gtaaaggttg gcagtggttc aaattcaaac
     3721 ttgttttttc caaccgtcaa ggtatcccca acctgataag taccggtatc gtaaaccccg
     3781 ataatatcac ctgccacggc attgatcaca ttctcacgac tttccgccat aaactgggta
     3841 acattagata gtttagcccc cttaccagta cgagggagat tgacactcat gccgcgctca
     3901 aattcgcctg atacgatacg gacaaaggca atacggtcac ggtgacgagg atccatgttg
     3961 gcttggattt taaagacaaa gcctgagaaa tccttgtcat aaggatccac aatttcaccg
     4021 tctgttttct tgtgaccatg tggttctgga gcaaacttga ggaagatttc aaggaaggtc
     4081 tgcacaccaa agtttgtcag ggctgaaccg aaaaagacag gcgtcaattc tccagccaga
     4141 atagcttcct ctgaaaactc attcccagct tcatttaaaa gctcaatgtc atccttgact
     4201 tgctcgtaga aaggattgct accaaagagt ttgtccccgt cttctagact ggcaaaacgc
     4261 tcatcccctt tgtaaagctc taaacgttgg ttatagaggt catacaagcc ctcaaaggct
     4321 ttccccatcc cgataggcca gttcataggg tagctagcaa tgcccaagat ttcttccaat
     4381 tcttgcaaga gatccaaagg ctcacgaccg tcacggtcca gcttgttcat aaaggtaaaa
     4441 actggaatgc cacgatgttt cacaacctca aacaatttct tggtttgagc ctcgatcccc
     4501 ttggcagagt ccacgaccat gaccgcagca tccaccgcca tcaaggtacg ataggtatct
     4561 tctgagaagt cctcgtgccc tggcgtgtct aagatattca cgcgcttgcc gtcgtagtca
     4621 aattgcataa cagatgaagt aacagaaatc ccacgttgct tctcgatatc catccagtca
     4681 gatttagcaa aagtccctgt tttcttccct tttaccgtac cagcctcacg aatctcaccc
     4741 ccaaagtaga gtaactgctc agtgatggtt gttttccccg cgtccgggtg ggagataatg
     4801 gcaaaggtac gacgtttctt aatttcttct tgaatattca taagttctct ttctttgatt
     4861 ctctattttt cttgtttcaa tagctgagaa tgatttttac attggatttt accattcctt
     4921 tcaatactcc attatatcgg attttagcat ttttttcaat ttctatctct tttcacttcc
     4981 ccctccctta tttataggaa aatatggtaa aatagaacag actaaaaatc atcatttcac
     5041 gaaaggatgc aagatgaaaa ttacgcaaga agaggtaaca cacgttgcca atctttcaaa
     5101 attaagattc tctgaagaag aaactgctgc ctttgcgacc accttgtcta agattgttga
     5161 catggttgaa ttgctgggcg aagttgacac aactggtgtc gcacctacta cgactatggc
     5221 tgaccgcaag actgtactcc gccctgatgt ggccgaagaa ggaacagacc gtgatcgctt
     5281 gtttaaaaac gtacctgaac aagacaacta ctatatcaag gtgccagcta tcctagacga
     5341 tggaggagat gcctgatgac ttttaacaat aaaactattg aagagttgca caatctcctt
     5401 gtctctaagg aaatttctgc aacagaattg acccaagcaa cacttgaaaa tatcaagtct
     5461 cgtgaggaag ccctcaattc atttgtcacc atcgctgagg agcaagctct tgttcaagct
     5521 aaagccattg atgaagctgg aattgatgct gacaatgtcc tttcaggaat tccacttgct
     5581 gttaaggata acatctctac agacggtatt ctcacaactg ctgcctcaaa aatgctctac
     5641 aactatgagc caatctttga tgcgacagct gttgccaatg caaaaaccaa gggcatgatt
     5701 gtcgttggaa agaccaacat ggacgaattt gctatgggtg gttcaggtga aacttcacac
     5761 tacggagcaa ctaaaaacgc ttggaaccac agcaaggttc ctggtgggtc atcaagtggt
     5821 tctgccgcag ctgtagcctc aggacaagtt cgcttgtcac ttggttctga tactggtggt
     5881 tccatccgcc aacctgctgc cttcaacgga atcgttggtc tcaaaccaac ctacggaaca
     5941 gtttcacgtt tcggtctcat tgcctttggt agctcattag accagattgg accttttgct
     6001 cctactgtta aggaaaatgc cctcttgctc aacgctattg ccagcgaaga tgctaaagac
     6061 tctacttctg ctcctgtccg catcgccgac tttacttcaa aaatcggcca agacatcaag
     6121 ggtatgaaaa tcgctttgcc taaggaatac ctaggcgaag gaattgatcc agaggttaag
     6181 gaaacaatct taaacgcggc caaacacttt gaaaaattgg gtgctatcgt cgaagaagtc
     6241 agccttcctc actctaaata cggtgttgcc gtttattaca tcatcgcttc atcagaagct
     6301 tcatcaaact tgcaacgctt cgacggtatc cgttacggct atcgcgcaga agatgcaacc
     6361 aaccttgatg aaatctatgt aaacagccga agccaaggtt ttggtgaaga ggtaaaacgt
     6421 cgtatcatgc tgggtacttt cagtctttca tcaggttact atgatgccta ctacaaaaag
     6481 gctggtcaag tccgtaccct catcattcaa gatttcgaaa aagtcttcgc ggattacgat
     6541 ttgattttgg gtccaactgc tccaagtgtt gcctatgact tggattctct caaccatgac
     6601 ccagttgcca tgtacttagc cgacctattg accatacctg taaacttggc aggactgcct
     6661 ggaatttcga ttcctgctgg attctctcaa ggtctacctg tcggactcca attgattggt
     6721 cccaagtact ctgaggaaac catttaccaa gctgctgctg cttttgaagc aacaacagac
     6781 taccacaaac aacaacccgt gatttttgga ggtgacaact aatgaacttt gaaacagtca
     6841 tcggacttga agtccacgta gagctcaaca ccaattcaaa aatcttctca cctacttctg
     6901 cccactttgg aaatgaccaa aatgccaaca ctaacgtgat tgactggtct ttcccaggag
     6961 ttctaccagt tctcaataaa ggggttgttg atgccggtat caaggctgct cttgccctca
     7021 acatggacat ccacaaaaag atgcactttg accgcaagaa ctacttctat cctgataacc
     7081 ccaaagccta ccaaatttct cagtttgatg aaccaatcgg atataatggc tggattgaag
     7141 tcaaactaga agacggtacg accaagaaaa tcggtatcga acgtgcccac ctagaggaag
     7201 acgctggtaa aaacacccat ggtacagatg gctactctta tgttgacctc aaccgccaag
     7261 gggttccctt gattgagatt gtatctgagg cagatatgcg ttctcctgaa gaagcctatg
     7321 cttatctgac agccctcaag gaagttatcc agtacgctgg catttctgac gttaagatgg
     7381 aggaaggttc gatgcgtgtg gatgccaaca tctcccttcg tccttatggt caagagaaat
     7441 tcggtaccaa gactgaattg aagaacctca actccttctc aaacgttcgt aaaggtcttg
     7501 aatacgaagt ccaacgccag gctgaaattc ttcgctcagg tggtcaaatc cgccaagaaa
     7561 cacgccgtta cgatgaagcg aataaagcaa ccatcctcat gcgtgtcaag gaaggggctg
     7621 ctgactaccg ctacttccca gaaccagacc tacccctctt tgaaatttct gacgagtgga
     7681 ttgaggaaat gcggactgag ttgccagagt ttccaaaaga acgtcgtgcg cgttatgtat
     7741 ctgaccttgg tttatcagac tacgatgcta gtcagttgac tgctaataaa gtcacttctg
     7801 acttctttga aaaagctgtt gccctaggtg gtgatgccaa acaagtctct aactggctcc
     7861 aaggggaagt cgctcagttc ttgaatgctg aaggtaaaac actggaacaa atcgaattga
     7921 caccagaaaa cttggttgaa atgattgcca tcatcgaaga cggtactatt tcatctaaga
     7981 ttgccaagaa agtctttgtc catctagcta aaaatggcgg tggcgcgcgt gaatacgtgg
     8041 aaaaagcagg tatggttcaa atttcagatc cagctatctt gatcccaatc atccaccaag
     8101 tctttgccga taacgaagct gctgttgccg acttcaagtc aggcaaacgt aacgccgaca
     8161 aagcctttac aggattcctt atgaaggcaa ccaaaggcca agccaaccca caagttgccc
     8221 ttaaactact tgcacaggaa ttggcgaagt tgaaagaaaa ctagacagaa caaaaccagc
     8281 cctaaggttg gttttttctt ctctaccaac tcccaataac tattttggct ttatttccag
     8341 agtattttat ggtaaaatga agagtaataa tatttattaa agaggtaaaa acatgattga
     8401 agcaagtaaa ttaaaagctg gtatgacctt tgaaacagct gacggcaaat tgattcgcgt
     8461 tttggaagct agtcaccaca aaccaggtaa aggaaacacg atcatgcgta tgaaattgcg
     8521 tgatgtccgt actggttcta catttgacac aagctaccgt ccagaggaaa aatttgaaca
     8581 agctattatc gagactgtcc cagctcaata cttgtacaaa atggatgaca cagcatactt
     8641 catgaataca gaaacttatg accaatacga aatccctgta gtcaatgttg aaaacgaatt
     8701 gctttacatc cttgaaaact ctgatgtgaa aatccaattc tacggaactg aagtgatcgg
     8761 tgtcaccgtt cctactactg ttgagttgac agttgctgaa actcaaccat ctatcaaagg
     8821 tgctactgtt acaggttctg gtaaaccagc aacgatggaa actggacttg tcgtaaacgt
     8881 tccagacttc atcgaagcag gacaaaaact cgttatcaac actgcagaag gaacttacgt
     8941 ttctcgtgcc taatctctag aaagaggtca ttctatggga attgaagaac aacttggcga
     9001 aatcgttatc gccccacgtg tacttgaaaa aatcattgct atcgctactg caaaggtaga
     9061 gggtgttcac tctttttcaa acagatcagt gtctgatacc ctttcaaaac tttcactcgg
     9121 ccgtggcatt tatcttaaaa acgtggacga agaactcaca gcagatatct atctctacct
     9181 tgagtacgga gtaaaagttc ctaaggtagc ggttgctatc cagaaagctg tcaaagatgc
     9241 cgtccgtaat atggctgatg tagaactcgc tgctatcaat attcacgttg caggtatcgt
     9301 cccagataaa acaccaaaac cagaattgaa agatctattt gacgaggact tcctcaatga
     9361 ctagtccact attagaatct agacgccaac tccgtaaatg cgcttttcaa gctctcatga
     9421 gccttgagtt cggtacggat gtcgaaactg cttgtcgttt cgcctatact catgatcgtg
     9481 aagatacggc tgtacaactt ccagcctttt tgatagacct cgtttctggt gttcaagcta
     9541 aaaaggaaga actagataag caaatcactc agcatttaaa agcaggttgg accattgaac
     9601 gcttaacgct cgtggagaga aacctccttc gcttgggagt ctttgaaatc acttcatttg
     9661 acactcctca gctggttgct gttaatgaag ctatcgagct tgcaaaggac ttctccgatc
     9721 aaaaatctgc ccgttttatc aatggactgc tcagccagtt tgtaacagaa gaacaataaa
     9781 aagaaaaagt gtttgatatc tatcaaacac tttttctttg cctcccacta tctaaaaaaa
     9841 tgataataga tataattgta aacaaaaatc cagataggtt ttgcatgatt gagaaagtta
     9901 aaaaaactat ggcagagaat cgttaatctc agattgtcgg tagaacgata aacaagggca
     9961 aaaaagaaac caatcagact ataatataat aaactaattg gatctctgtg agatagtatc
    10021 aaatgactaa gcccaaagat aaggcctgat aagatgacat ctaaataata cttggactag
    10081 ggaaagaagg tattcataaa ataccctcta tcaagagtct cctcaaaaac aggaccgatg
    10141 attacaggca ggacaaaaga taagatagtc gataaaaagg ttggttgtcc atttgaaaga
    10201 accacggtaa aatactcatc atgaatattc ctatgattaa tcaaatgagc atagcgtgcc
    10261 caaaaattac cgagaatctg ataaaccaca taagttgcaa ataaggagaa gacaaatgac
    10321 cagttccagc tctttttctt ttttaacctc caaattaata gaaaaggaaa cttcccacta
    10381 atcccactgt taaaataaga gaatagacat cagctcctaa ccctaaaatg atcgtcacat
    10441 acaatccaat tgtttgtggt aaataggtag atagtaaaat aataagcaaa aatattccaa
    10501 attgtcttag tttctttgtg tttctcatcg tacttttttg aaagattacc ctgttcggaa
    10561 gccgtacttc caagcatcta tataagaatt aagtgcccct tgcctcatat agggagcaaa
    10621 ttctctataa tataaccatc tactatatcc atcttcccaa acagcaagac cacctgaagt
    10681 ttgctccaag tcctcagttg aaataactgt aaatgtttct gtacctgtca ttgcaagtac
    10741 cttcttaaaa tagattgttg taggctcaca tttatagtat atttcttttt ttgtctattt
    10801 tatagtctat ctcctcaact ggcaattttt cgacctgaat tgcatttttc cataaaaaat
    10861 gagacctttc tagtctcatt tagtcattct tagtattttc taaatcgttg atagcgttct
    10921 tccagcaact cttctagcgg tttttgtgaa agtctagcca gctccgtttg gagttctttt
    10981 ttgacactct taattagttc tttactagaa agtcctactt cagaaatcac cttatccacc
    11041 acgtccattt ctaacagttc atgcgaagtg attttcatca gttctgctgc ttccatggcg
    11101 cgagtaccgt ccttccataa aatggaagca aagccttctg gactgagaat ggcatagata
    11161 gaattttcca gcatccagac acggtccgcg acagctagag ccagagcccc gcctgaacca
    11221 ccttcaccga taataatggc gataatagga actttcaggt cactcatttc catgagattg
    11281 cgagcgatag cttccccttg accacgttct tccgctccga caccaggata agcacctgct
    11341 gtattgataa aggtcacaac tggacggcca aatttctcag cctgtttcat caaccgcagt
    11401 gcctttcggt agccttctgg atgtggttgg ccaaaattcc gtttgaggtt gtcttgcaaa
    11461 ctcttgcctt tttggatacc aaccactgtt acagcttggt ctccaagcca accaatacca
    11521 ccaacaactg caccatcatc acgaaaagaa cggtcaccat gtaattggat aaattcatca
    11581 aaaatgcctg tcgcaaagtc caaggttgtc aagcgactct gctcacgcgc ttctctgact
    11641 atttttgcaa tattcatcta ggactccctc catgcaatct gactaggcta gcaatcgtat
    11701 ctggtaagtc tcttcttttg acaatagcat ccacaaagcc atgttctaat aggaattctg
    11761 ccttttggaa atcctcaggc aagctttcac gaaccgtatt ttcaatcaca cgacgcccag
    11821 caaaaccaac caagctctgt ggttcagcca gaatgatatc gccttccata gcgaaagaag
    11881 ctgtcacacc accagtcgtt ggatctgtca aaatggtcag gtaaaagaga ccagcatttg
    11941 aatggcgttt aaccgctgca gagatcttag ccatctgcat gagactcatg attccttcct
    12001 gcatacgggc tccaccagag gctgtgaata ggacaactgg caatttttcg acagtcgcat
    12061 actcaaacaa acgagtgatt ttttcaccta caaccgtacc catagaagcc atgataaaat
    12121 tagaatccat aatcccaaga gccacagtct gacctttaat aagagcagtt cctgtcacaa
    12181 cggcttcatg cagacctgtt ttttcacgca tagatgccag tttcttttgg taaccaggga
    12241 aatgcaaggg atccttgctt tcaatccctg taaacaattc tttgaaggtt cccatatcaa
    12301 tcgtcaaagc caagcgttct tgggcagaaa tacgaaaggt atagctacag tgcggacaga
    12361 tacgttcact tcccagatcc ttctgataga tggtatgctt acagcctgga cactgggaaa
    12421 ataattcatc tggaacctct ggcttagctt gaggtttttc cctaaccgaa cgattgggat
    12481 tgattcgaat atacttatct tttttactaa atagagccat tgattcccct tttcggttta
    12541 aactcttaaa gtcattttat tctttttctt gatatttagg taagaaggtt tccatcaaga
    12601 aggaagtatc ataatcccca gcaatgacat tgcgatctga aatgagatca agctggaaat
    12661 ctgcattggt ctgcactcct tcaatctcta attcatagag ggcacgttgc attttcatca
    12721 aggcgtcaaa acgattttcg ccgtgtacta tgattttggc aatcatacta tcataataag
    12781 gcggaatggt ataacctgga taaactgctg aatccacgcg caagccaact ccaccacttg
    12841 gcagatagag attagtaatc ttacctggac ttggagcaaa gttaaaggct gggttttctg
    12901 cattgatacg acactcgatg gcatgaccgc gtaggacaat atcttcttgc ttaacagaca
    12961 aaggctgacc tgccgcaatg caaatctgtt ccttaacgat atcaacacct gaaacaaact
    13021 ctgttactgg atgttctacc tgaacacgag tattcatctc catgaaatag aaatttctac
    13081 ttgcttcatc aagaagaaat tcaatggttc ctgcattctc atagccaaca aactctgccg
    13141 ctcgaacagc agcagcacct atttcatgac gcagcgtttt tccgattgca atcgagggac
    13201 tttcttccaa aaccttttgg ttattccttt gaagagaaca atcccgttca cccaagtgaa
    13261 tcacatgtcc atgctcatca cctaggattt gaacctcaat gtgccgagct ggatagataa
    13321 cccgttctat gtacatggca ccattgccat aattggcctt ggcctcacta gaggcagttt
    13381 caaaggcaga aacgaggtca tctggttttt caaccttacg aatcccttta ccacctccac
    13441 ctgctgaagc cttgagcata acaggatagc caattttttc agcaacaatc aaagcttctt
    13501 cagagttatg cacttctcca tctgaacctg gtataacagg cacacctgct ttaatcatct
    13561 gagcacgcgc attgatctta tcccccatca tatccataac atgaccagat ggaccgataa
    13621 acttgatacc tacttcttca cacatggtcg caaatttgga attttcactg agaaatccaa
    13681 aaccagggtg aatagcttct gcctcagtca agactgcagc tgatagaact gcattaatat
    13741 tgagataaga ctctgttgcc ttgccaggac caatacaaac tgcttcatct gccaaaagcg
    13801 tatgaagagc ttccttatca gcagttgaat aaaccgctac cgtcgcaatc cccaattcac
    13861 gtgccgcacg gataatacga accgcaattt caccacgatt ggcaattaaa atttttcgaa
    13921 acatggagaa cctccttagt tcccaattgc aaaagtaagg ataccactgg ctgcaagctt
    13981 gccatccact tcagcctttg cttcaaccac agctatggtg ccacgacgtt ttacaaaagt
    14041 cgctgtcata accaattggt cgcctggtac aacttgcttc ttgaacttaa ccttgtccat
    14101 accagcgtaa aagaccagtt ttcctttatt ttcaggtttt gataactcca acacaccagc
    14161 agtttgcgcc aaggcttcca taatcagaac acctggcata actgggtatt gaggaaagtg
    14221 gccgttaaag aaaggttcgt taatggtcac atttttgata gcaacaatgg tatcctcgct
    14281 cacttccaag acacggtcca ctagaagcat aggataacgg tggggaatag cttctttgat
    14341 tccttgaata tcgatcattt gatacgtacc aatcctttac caaactcaac catttcttcg
    14401 ttagagacga gaatttccgt taccacacca tccttaggag ctgggatttc attcatgact
    14461 ttcatggctt cgataattac caatgtttga ccttttttga cactatcacc aactgtaacg
    14521 aaggcaggtt tatctggtcc agcagccaag taaaccactc caacaagtgg actctctaca
    14581 agatttccct cagtagccac acttgcttca gctggagctg gaacttcttc tgctacagtc
    14641 tctgctggag cagatgtagg agctactgga ctcggtgttg ctagaacggg tgctggagcg
    14701 acttgagttg caacttcagg cacaggtctt gcttcattct tgctaaactg caactcatcc
    14761 gtcccatttt tataagaaaa ttctctcaaa cttgactggt caaattgagt catcaagtct
    14821 ttaatatcgt ttaaattcat acttatctat tctcccaacg tttgaaagca agaactgcat
    14881 tgtggcctcc aaaaccaaaa gtatttgaaa tagcgtatgg aatttctttc tccaaacctt
    14941 gtccataaac gacattagct tcgatataat ctgatacttc acttgtccca gctgtcattg
    15001 gtacaaagtt atgacgcata gcttcgatgg tgacgatagc ttctactgca cccgcagccc
    15061 ccagcaaatg tcctgtaaaa gacttggttg atgatacagg tacttcctta ccaagaacag
    15121 ctacgatagc accactttct cctttttcat tggcaggagt tgacgttccg tgagcattaa
    15181 cataggctac ttgctctgga gaaatctcag cttcttccaa ggctagtttg atggccttga
    15241 tagctccctg accttctgga tgtggagaag tcatgtggta ggcatcacaa gtatttccgt
    15301 aaccaaccac ttcagccagg atagtagctc cacgtttttc agcgtgttca agactttcta
    15361 gaaccaacat ccctgaacct tcacccataa caaacccatt gcgatcctta tcaaatggga
    15421 tcgaagcacg agttggatcc tctgtagtag agagagctgt taaggcttgg aaaccagcga
    15481 tggcaaaagg tgtgatagaa gcttctgttc ctcccaccaa catcacatct tggaaaccaa
    15541 acttaatgga gcggaaggca tccccaatcg catcatttga tgaagagcag gcagtattga
    15601 tagatttaca aacaccgttt gcaccaaaac gcatggctac attcccagaa gccatatttg
    15661 gtaaagcttt tggaagagtc attggtttga cacgtttggg tcctttttca tgaaggcgaa
    15721 gtacctgatc ttcaatttcc ttgattccac caataccaga tgcaacgata acaccaaaac
    15781 gatccctatt aagagcctct acatcaagat tggcatggtt tacagcctct tgggctgcat
    15841 acaaggcata taaagaatag ttatcaaaac ggttggtatc tttttttaca aagtatttat
    15901 cgaacggaaa atcttggatt tctgccgcat tatgcacatc aaagtcacta tgatcaaatt
    15961 ttgtaatgcc accaatgccg attttcccag ttgctaaact attccaaaat tcttctggtg
    16021 tatttccgat tggagatgtt actccataac ctgttaccac tactcgattt agtttcattc
    16081 ttttcacctc tagctttcgc tacatactta agccaccatc aatggcaatc acttgaccag
    16141 ttagataatc ttggcctgct aaaaatactg tcaaatctgc aacctgctct gcctgcccaa
    16201 attctttcat cggaatctga gctagtgtag cttccttaat cttatctgat aagatagctg
    16261 tcatatcaga ctcaatcatt cctggagcaa tcacattgac tcgtatattc cgactagcga
    16321 cctcgcgtgc cacagacttg gtaaagccaa tcaagccagc cttagaagca gcatagttag
    16381 cttgaccaat attccccatc aaaccaacaa cactagacat attaatgata gcaccttctc
    16441 tggctttcat catcggtttc aagactgatt gtgtcatatt aaaggcacca gtcagattga
    16501 ccttgagcac tttttcaaaa tctgcttctg tcatcttgag cataagagta tcttgggtaa
    16561 tccctgcatt gttgaccaaa acatctactg aacccagttc tgcaatagct tgatcaatca
    16621 tacgcttagc gtctgcaaaa tctgatacat ctcctgaaat gggaaccacc ttgataccat
    16681 agtttgaaaa ctcagcgagc aattcttctg agattgcccc acgactgttt aagacaatgt
    16741 tggctcctgc ttgagcaaac ttgtgggcga tggcaagacc aattccacga ctcgaacctg
    16801 taataaagat atttttatgt tctagtttca tttttttcct ttcaaaactt ctacttattt
    16861 tagtctattt ttttaaaagt gctactaaac tcgcttgatc ttccacatga gctaagtgag
    16921 tagtttgatc aattttttta acaaaacctg acaagacttt ccccggtcca atctcgataa
    16981 agttgcttat gcctgcttct tgcatgaccc caatactttc atagaaacga acgggttcct
    17041 tgacctgacg cgtcaagagc tgagcaatgt cctctttttg catcacagca gcttctgtat
    17101 tgccgactag gggacaagta aaatctgaaa aacttacctg agccagagtt tcagctagtt
    17161 tctggctagc aggttcaagg agagaggtgt gaaagggacc tgacacctta agaggaatca
    17221 agcgtttggc acctgcttct tgcaaaagtt caaccgctcg atcaactgca accacttctc
    17281 cagcaatgac gatttgtgca ggtgtgttat agttggctgg agtaaccact ccaagttcag
    17341 aagctttttg acaggcttct tcaatgacct ctactggcgt attgagaact gctaccatct
    17401 tgccagagtc agcaggagcc gcttcttcca tataggctcc acgcttagct accaaggcaa
    17461 ccgcatcttc aaaatccaag gcgccgcttg ccaccaaggc agagtattct ccaagagaca
    17521 aaccagcaac catatcaggc tgatagccct tttcttgcaa taaacggtag atagcaaccg
    17581 aagtcgctag aatggctggt tgcgtatagc gggtctgatt gagtttgtct tcttccgtat
    17641 cgatgagata acgcaaatca taacctagca cctgactcgc tcgatcaatc gtttctttaa
    17701 caatcggata ctgatcatag aaatcccgtc ccatccctag atactgggca ccttgaccag
    17761 caaataaaaa ggctgtttta gtcatttctt acaactcctg tccagcgaga ggcttcttct
    17821 tgaattttct tagcggctcc gtaatacaaa tcttttagga tttcttcagc tgtttcttct
    17881 ttagaaacaa gccctgcgat ttgacctgcc atgacagagc caccctccac atcaccgtga
    17941 acaactgctt tggctagggc acctgctccc atttgttcaa agatttctaa atcaggatct
    18001 tcctgcttaa aggcatcttt ttcagccagt tcaaaatctc tagtcaactg atttttaata
    18061 gcacgaacag catgaccaaa gtgctgagct gaaatcgtag tgtcaatatc ccttgctttt
    18121 aaaattttct ccttgtagtt tggatgggca ttcgactctt ttgcaactac aaaccgtgtc
    18181 cccacctgta cagcctctgc acctagcata aagccagccg cagcaccttc accatccgca
    18241 attcctcctg cagcaataac aggaatagat acagctgtgg ctacctgtcg caccaaggtc
    18301 atggttgtta atttaccgat atgcccccca gcttccattc cttctgcaat aacagcgtct
    18361 gcaccgattt tttccatgcg tttagctaaa gcgacactag gaacgacagg aataacgatt
    18421 atcccagctt catggaaacg ttccatatac ttgcttggat ttcctgctcc tgttgtgaca
    18481 actttaacac cttcttcaat aacgagatcc acgatatctt ccacaaaggg agataagagc
    18541 atgatgttga ccccaaaggg tttatcagtc aatgatttga ttttatcaat attggccttg
    18601 acaacttctt tcggggcatt tcccccaccg ataattccta atcctccagc cttggaaaca
    18661 gcccctgcca aatcaccatc agcaacccag gccatccctc cttggaaaat aggatagtca
    18721 atcttcaata attctgtaat acgcgttttc atagtgcctc caaccttcct tgcttacgta
    18781 atagttcgat ttcaccataa tttgacagtc aaactattac ctaaacaaga gggagtgggt
    18841 ttctccctac tccttctact aatattctgc ttattttgct tgctcttcaa cgtaagcaac
    18901 caagtcacca actgttttca agtcattttc tgcttcgatt tggatatcaa aagcatcttc
    18961 gatttctgag attacttgga acaagtccaa tgaatctgcg tccaaatcat caaaagttga
    19021 ttcaagtgtt acttctgatg cgtcttttcc aagttcttca acgataattt cttgtacttt
    19081 ttcaaatact gccatgatag gactccttta aaataaatag tttttttata acaatgtgtt
    19141 caccacatga ttacctaaat tgtaagaatg agcgtgcccc aggtcaaacc tccaccgaag
    19201 cctgatagaa gaacagtctg gctaccatct aaagggatga gaccttgttc tacacactct
    19261 gaaagtaaaa tcgggatact ggctgcactg gtattgccat attccatcat attggctgga
    19321 agtttggctc ggtcaacacc aatttttcta gccatcttat ccaaaatacg gtcattggct
    19381 tgatgaagta gcagataatc caagtctgtc acctctatag gagattcatc aatagtctgc
    19441 ttgatagact tggctacatc tcgaatggca aaatcaaaga ctgtgcgtcc atccatcttc
    19501 aaaaacgaat ctgcactttc ttgatctgaa aatggagaat gcaaacctga atgcccataa
    19561 gttaaacact cgctgcgact tccatcgcta ttgagactct cagctaagaa atgctcttgc
    19621 tcgctagctt ctaacaagac accaccagca ccatctccaa acaacacagc tgttgatcga
    19681 tccgaccaat cgactgcctt agagagggtt tcactaccaa tcaccaagcc tttttgaaag
    19741 cgaccagaag cgataaactt ttcagcagtt gaaagagcaa atacaaatcc actgcaagcc
    19801 gcggttaagt caaaagcaaa ggctttatta gcaccaatat tagcttgaac acgagcagct
    19861 gtagagggca tcatcgaatc tggagtaatg gtagctagga tgataaaatc cagttcttct
    19921 cctgttattc cagcttttgc catcagtttc ttagcaacct ctgtagccaa atcactggta
    19981 gattctgttc ttgaaatatg cctttgtcgt attcccgttc gacttgaaat ccactcatca
    20041 ttggtatcca taatctgagc caagtcgtga tttgtaacca cttgctctgg cacataatga
    20101 gcaacctgac ttatttttgc aaaagccatt atttcaaatc ctccaaaaat tggtaaagat
    20161 tagtcaagcc tttacccatg acagcaattt cttcctcgct catgccatca ataatttttt
    20221 ctaccatggc cttgtggaag cgtttatgca gtctatgaat caagcgaccc ttctttgtca
    20281 aatgcagatg caccacacga cgatcctgtt ctgaccgaac tcgctcaatg tagcccttac
    20341 gctctaaatt gttcaaactt gtcgtaacag ttccaagagt taccattaac tcttttgaca
    20401 cttgacttgg agtcacatct ggagccttac cgatgacatc aattgtatgc atttctttga
    20461 tggagatatc cttaaaacga ctacctctca agttcacttc ctcaattaca aggacattgt
    20521 taaatataga tgttaaatat tcattgattc gttggtagtc catttttcat atccctcctt
    20581 cttcaaaaaa cttttacaat caaaacattt gacagaaaga attatatcaa aattcaaaga
    20641 atgatgcaag tattttttat tttcctgtaa atttaggtct tcttctctcc gaatgagctc
    20701 gcactccttc tttgaaatcc tctgtttgag ctaaggattt ctgtaggttc agttctaaag
    20761 tagcgtaacc ctgccagtct ttaaattggc tctcccaaac caacttctta atggcagcat
    20821 aggaattact tgaagcacgt cttaattttt taagaagctg ttctctcgtc ttttcaagtt
    20881 tttcagcttc agaaacgcgg taaaccaacc cccattctag ggctttttct gccgttaaag
    20941 cctctcctgt catagctaat tgagcagcac gcgtcacacc gatactgcgg ctcaagagat
    21001 gaattccccc tgcatctgga gccaaaccaa caccaacaaa ggcttggata aacttagcct
    21061 tatccgtcgc caaacagaaa tctgccgcaa cagccatatt cgctgcggca cctgcaacag
    21121 caccgtcaac ttccattaaa acaggtttgg ctatttgctt gattttataa gaaatcgtat
    21181 tgaccaactc tgcgattttt gtcaatgatg gaatatcatc ctcatccact gcccgcttca
    21241 tctctaccaa atctccccca actgagaaga cctttccatt ggcattgatt aagataaaat
    21301 gcacagctgg attttcttct gccaaagtca gagcttctaa aatctcctca cacatgggaa
    21361 tatgaaaacc atttgcgacc tcaggacggt tcaaggtaag gattgccaag tcctcttcaa
    21421 gctgataaat aatgtgttcc atcagggctc ctttttattt tataagcagt ttatactctt
    21481 cgaaaatctc ttcaaaccgc gtcagcttcc atctgcaacc tcaaaacagt attttgagct
    21541 gacttcgtca gtcttattta caacctcaaa gcagtgcttt gagcagtctg cggctagctt
    21601 cctagtttgc tctttgattt cattgagtgt taaattattt cactgtcaaa gtatatcttt
    21661 tttaggagca gaaagcaaga aaaaatcgac tgaaagtgtc tcagccgata agaaaggctc
    21721 gctttaggct agaaagcatc ttattttctt cattgaaaaa agcctttctt tctgctataa
    21781 tcgtataaaa tacttacttt aggagttctt atgaaagttg ttaaatttgg aggtagttct
    21841 cttgcctctg ctagtcaatt agaaaaagtt ttaaacatcg tcaaaagcga ttcagagcgt
    21901 cgttttgtag tcgtttctgc gcctggtaaa cgcaatgctg aagatactaa ggttacggat
    21961 gccctgatta aatactaccg cgactatgtt gcgggtaacg atattagcaa gagccaaagc
    22021 tggattatcg accgctatgc tgctatggtt agtgaattgg gactaaaacc agctgtgcta
    22081 gaaaaaattt ctaaaagcat tcacgccttg gccactcttc ctattgaaga aaatgaattt
    22141 ctctacgata ctttcctagc agccggtgaa aataacaatg ccaaattgat tgctgcctac
    22201 tttaaccaaa atggtatcga tgcacgctat atgcacccta gagaagctgg gattgtggtc
    22261 acaagtgaac ctggtcacgc tcgcatcatt ccatcaagtt atgacaagat tgaagaattg
    22321 acaaacacca atgaagtcct tgtcattcct ggtttctttg gtgtcactaa ggaaaatcaa
    22381 atctgtactt tctcacgtgg aggttctgat attacaggtt ctatcattgc tgctggtgtc
    22441 aaagccgacc tctatgaaaa ctttacggac gttgatggta tctttgcagc ccaccctggt
    22501 attatccacc aaccacactc gattcctgag ttgacctacc gtgaaatgcg cgagttggcc
    22561 tatgcaggct tctcagtcct tcatgacgag gctcttcttc ctgcctaccg tggaaaaatt
    22621 cctctggtta tcaagaatac caacaaccct gaccatccag gtactcgtat cgttctaaaa
    22681 cacagtaatg atgaatttcc agttgtggga attgctggtg actcaggctt tgtcagcatt
    22741 aacatgtcga aatacctcat gaaccgtgag gttggatttg gccgcaaggt tctgcaaatc
    22801 ctggaagaac ttaacatcgg ttgggaacat atgccaacag gtatcgacga tctttctatc
    22861 attctccgtt ctcgccaact aactcccatc aaggaagaag aaatcctgcg tcagttggtt
    22921 caaaaggcta aagtagatca tgcagaaatc gaacacgacc tttctatcat tatgattgtt
    22981 ggtgaaaaaa tgaagagcca catcggagtg actgctactg cgacacgcgc tctatctgaa
    23041 aacaaaatca acatccagat gatgtctcaa ggttctagtg aagtttctat tatgtttgtt
    23101 gtcaataaag accaagagaa agcagcaatt aaagccctct acaatgcctt ttttggagaa
    23161 agtaaggaag actaagcatg gcccaatcac ttaacaaaac agtactcctc agtacgacag
    23221 gcacttccta cctctctatc gctgggaaag ttgggaaatt ccttgtcgga gatcaggctt
    23281 tggaatttta cccagatgtc aatgtcgaac aatttatcca gattccttgg agccatatca
    23341 atcagattgg agccaatgtc actggacgca aaatcagccg ccacttcgaa gtctttacag
    23401 acaaaggaaa attcctcttt gcttcaaaag actcaggtgc cattctcaaa attgcacgcg
    23461 aaaaactagg caatgacaag gtcgtcaaac ttccgaccct gattcagaca attagccaaa
    23521 aatttaaaaa tctatttgca aaaaagtaga aactttagta taatcatagc aacggataag
    23581 taggttatct tcttctttca gaaagtctgc gggtactgcg agcagatagg ggggataggt
    23641 gaaattctac cgttagtaac aattacatac acaatcaagt gcacacctgt gaagttggat
    23701 ggaaccgtgg ccctgccact ccaacgtttt gtcaggcgtg cttttttcat aaaggagttc
    23761 ttatgttaga tatcaaacgt attcgtacag attttgaagc tgtcgcagaa aaattagcta
    23821 cacgtggtgt agaggctgct gtcttgaatg aaatgaaaga aatcgatgct aaacgtcgta
    23881 acatcttggt caaggttgaa actctcaaag cagaacgtaa cacagtttct gctgagattg
    23941 cccaagctaa gcgcaacaag gaaaatacag atgacaagat tgctgccatg caaaatctat
    24001 ctgctgaggt taaagccttg gatgctgaat tggcagaaat cgatgctaaa ttgacagaat
    24061 ttacaacgac tcttccaaat atcccagctg acagcgttcc tgttgggact gacgaagacg
    24121 acaatgtgga agttcgccgt tggggtactc cacgcgagtt tgacttcgaa cctaaagctc
    24181 actgggatct cggtgaagac cttggtatcc ttgactggga acgcggtggt aaggtaacag
    24241 gcgctcgctt cctcttctat aaaggcctcg gtgctcgttt ggaacgtgct atctacaact
    24301 ttatgttgga tgaacatgga aaagaaggct atactgaagt catcacacct tacatagtca
    24361 accatgattc tatgtttggt actggtcagt atccaaaatt taaggaagat acttttgaac
    24421 tcagcgatac caactttgtc ttgattccaa ctgctgaagt tcctctgaca aactactacc
    24481 gtgatgaaat cttagacggc aaagatcttc caatctactt cactgccatg agtccgtcat
    24541 tccgttctga ggctggttct gccggtcgtg atacgcgtgg cttgatccgt ttgcaccaat
    24601 tccacaaggt tgaaatggtc aaatttgcca aaccagaaga atcttacgaa gaattggaaa
    24661 aaatgacagc caacgctgaa aacattcttc aaaaactcaa ccttccatac cgtgtcgttg
    24721 ctctctctac tggagatatg ggcttctcag ctgcgaagac ttacgacttg gaagtgtgga
    24781 ttccagcaca aaacaattac cgtgaaatct caagctgttc aaacacagaa gatttccaag
    24841 cccgtcgtgc ccaaatccgt taccgtgatg aagcagatgg caaggtgaaa ctccttcata
    24901 ccttgaacgg ttctggactt gcagttggac gtacagtggc tgcaattctt gaaaattacc
    24961 aaaatgaaga tggttctgtg accatcccag aagcacttcg tccatacatg ggtggagctg
    25021 aagtcatcaa accataaaaa ataaggttta gctatttcta gctagacctt ttttcgtaac
    25081 caaatcagat aagcacctag tacaaagaat aaaatagtta ggcatataat ggtttcagcc
    25141 aataccaggt aatccagaaa tggaagtttc aaaattccct gagccatctt gagcgaggtc
    25201 gctgtgataa tggttgggaa ggtgagggct gagaaggctg gttgaaaacc ttgttttaaa
    25261 atgttgggca gacgagttaa aacaaagaaa aagaaggatt gagaagccaa aatcatgaca
    25321 atcaagaccc aagtcggcag gctggttcct cctactcgaa ctagagaagc caagagtaga
    25381 gagaaaggag cacagtagat tccttcttgt ccaagcaagg ctagtgggag tggatgtttc
    25441 tttaaatcgc tataaataag gggatagaga tagaaggtca agagaaaacc aaaactcaag
    25501 gtcgcatagg caatttcgat aatacctacc agaggatagg tcaaggcagc cactgctatc
    25561 cccacataga gaaccgtcca acttggagtg gcatgaaccc tccgccctga acaagcaaac
    25621 ttgatggtaa aaccagcaat caaggtcaaa tccaagagaa atgaaaacca ccaaatccct
    25681 tgtgctacca aaggaagata agagaatacg cgaaagacat aggtcgataa aatcatccca
    25741 gccataggaa aggttgccat tcctgacaaa agagggggct tggtcaattc ttgcttggtt
    25801 tctttccaat taaagagatg cagaattaga aagtaaatcc ataaaaccaa accagtcaga
    25861 ctaaaaagat gggatagaac cggcaacgta tctaaaataa gatttccagc tcctgccaaa
    25921 cctagcaaac aacctgaaaa tactaagggg agttttttca tcctaacctc caataatcat
    25981 gttagtttca gtataacata aaagcgctta aatgaggatt taaaaaaacg aagtccgctt
    26041 atttcagact tcattttact cagatatgaa ttaggcataa ggttgcaatt ctggattaat
    26101 tggtgtatta gctaagttgt tggcatagtt acagaggatt gctaggctga caccaaaaac
    26161 cacatccaag gcattttgtt gagtgtagcc agcttctaaa aactcagaca aggcttcatc
    26221 tcctacacga cccttggtat tgataactgc caaggtaaac ttagctaggg tatccaattt
    26281 aggatctgtt tcaattggag tacgattgcg aagagcttga atcaagtcat cattcatctg
    26341 gatttgtttg atggaaaagg ctgtgtgacc tgcgacacag aaggcacaac cattggtcac
    26401 ggctgccgtg atttgcacca cttcacgctc aacgggtgtc aggctgttgc gacggtggat
    26461 agatgagaca atttggtagg cttctaaaac agtcggggca ttggccaaga gaccgattag
    26521 gttgggaata tagccattgt tgtctttttc tactgtttca agaatttctt tcacttctgc
    26581 tggtgctgac tctactgtat ggatagtaaa tgttgtcata agatacctct tttcttatta
    26641 ttgacactaa tattattgga aaatcttata aaatcctgat tcctaagttt atctaagata
    26701 aagctttatt ctctcataag attttcgttg ttatattagt ttatcacact tccaatcact
    26761 tgcataatat atattatata tcaggctgat aaaaattatt tataggcaaa aaaatcacac
    26821 gagctgtgtg attccattat ttgtcaaaat actttttagt ttcagcaata acgactggcg
    26881 acaagaccaa gagggcaatc aagtttggca gagccatcaa ggcgttaacg atatctgcga
    26941 taatccagac catatccaac tcgataaatc ctcctaacaa gaccatgagc acaaaaaaca
    27001 cacggtagag ccagataaag cgagccccaa agaggaactc aaaacagcgt tctccgtaat
    27061 agttccaacc tagaatcgtt gtaaaggtaa aaagtacaag gaagatggtc aagagggcag
    27121 gcccaaagtg tgaaaagact gttgagaaag ctgactgagt caaggcaacc ccattcaagt
    27181 caccactcca aactccagtt accaagatgg tcaaaccagt tagagtacaa atgatgaggg
    27241 tatcaataaa ggttcctgtc atggaaatca aaccttgctc tactggttca tttgtcttgg
    27301 cagctgcagc tgcaatagga gcagaaccca gaccagattc gtttgagaac acaccacgcg
    27361 ccacaccatt ttgaatagcc atccgaacgc tagcaccagc aaatccacct accgcagcaa
    27421 ggggactaaa agctgaggta aagactaaag cgattgtgcc agggattttt ccgatattaa
    27481 agaaaataac tgtaagagtt cctaagatat aaatgagggc cataaaagga acaacagtag
    27541 ttgaaacctt agaaatagac ttgagtccac caaagactgc aatcgctaca aagacagaca
    27601 agacgagagc tgtgatggct ggcgaaatcg tcgttgtatt ttggatagat tctgtaatcg
    27661 agttgacttg ggtgaaggtt ccgattccca agagagcaac caatactcct gctactgcaa
    27721 acaaaacagc aagtggtcgc cacttttctc ccatccctag aaggatatag tgcatgggac
    27781 ctcccgctac tgcaccatgg tcgtccttgg tgcggtattt gatggccaag agtccttccg
    27841 catacttggt agccattcca aagaaagccg ccatccacat ccaaaataga gctcctggtc
    27901 caccaacctt gatagccgtc gcaactccta tgatatttcc tgttccaaca gttgatgcca
    27961 aggctgtaca cagagctgca aaactggata catcaccatg tcccttatcc tggataaaaa
    28021 taagctgaaa ggccttgggc agacgcaaaa cctgcaagag tcctagccgc atggttaggt
    28081 aaatccctgt tccgaccaat aaaatcaaga ggggcggtcc ccaagcaaaa gcatcgattg
    28141 atttaagcaa ttctaacatt ttcttctcct atcgtttcaa ccccaaaaga aagagcacat
    28201 gcaagataca tgtactctgg aatgcttaga taaatgctaa aaagcggtct atcctagctc
    28261 tgtcctttta cctgagagtt tgagcagttg cctgccttgc cccttcggtg cctttacggt
    28321 ctctccagag ttccgtccat ttacagtcat ggaaaatcaa acgattcccc acttctatta
    28381 aacttcattc ggtgttggta tttaattgat tctaatttta caaaaaatgt tggcttttgt
    28441 caatgtgttt attagtaaaa attagttcaa cagtttttac tttataaagt ccagaatact
    28501 gctatccttt aaaagtgaca atagtcgcac cactgcctcc agcattttgt ggggcatagc
    28561 cgaaactctt gacatgtttg tttctttgca agtatttggt aactccttca cggatgactc
    28621 ctgttccgat accatggatg atatcaactt gagccatatt gttaagcaag gcttggtcga
    28681 tgaaggtatc tagctcattc atggcttctt catagcgctt gcctcgaaga tccagtctag
    28741 cttgaggtcc tcgcccagaa gttcgtttca caacattgac ctgtttcttc ttgactggtt
    28801 tttcttgctg ggcttgaaca agatcaaact ctttctcttc caaggtcatc ttaatcaagc
    28861 caatttgggc ttcccagcgg ccgtccttga gttgactggt caaggtacca cgctgaccat
    28921 aactgagaac cacgatatca tctcccacct ttggagctcg ttttttcttg gccttttgaa
    28981 ggaccttgtt tttagacaag tccacttttt caggagccaa ttttttcaac ttggccttgg
    29041 cttcaatgat ttcgtggggc ttgagttggg atttactgtg gagatttttg agaatctggt
    29101 cactttcact tagggccata tccacaatct cagcagcctg ttcacgcgcc ttgttaagct
    29161 cggtttcctt ttcacgatta agctcgttgt agagtttttt tagcgcacgg ttcatcttga
    29221 gattttcttg ctccacctca cggatattgt ccaagcgttt gcggctttcc agcgtctgct
    29281 cttctaattg ctcaatgata cgattgacgt cattgtcctg atcgatctgc tgactggcat
    29341 ctcctacgat aacttcagat aggcctagac gtttggcaat ttcaaaggca ttacttcggc
    29401 caggaacacc ctgcataaag cgataggtcg ggcgaagagt tgcagtatca aactccatac
    29461 tggcattttg cacaaaggct gtctcaatac cgtaggcctt gagttctgga tagtgggtcg
    29521 tcgccatggt cttgatttga cgcaggcgaa ggtcctccag aatagccatg gcaagggctg
    29581 ctccctcttg gggatcagta ccagccccca actcatccaa aagtaagagt gaatgttggt
    29641 tgaccttgcc aagaatatcc acgatattgg tcatatggct agagaaggta gacaagctct
    29701 gctcaataga ctgctcatct ccaatatcag caaagatttc ttcaaaaata ccaacacgac
    29761 ttcccttgtc tgctaaaatc ggcaatcctg actgggccat gacctgtgtc aagcccagag
    29821 ttttaagcat gatggtcttc ccacctgtat tgggacctgt aatgacaata gctgttaaat
    29881 cttgaccaaa atagacatca tttgcgacgg catttttgac caaaggatgg cagacatgga
    29941 gcagttgaat ctcttgattt tctgacagct gaggcacgac tgcttgtctt tcttgaataa
    30001 atcgaacctt ggcacgaatc aagtccagat gaccgataat ccaagcgtca ttagcaatct
    30061 cagccgcatg agggcggaca cgctcagaaa tttcttggag aatgcgaagc atttcatagc
    30121 gctcatctgc tcgcagacta gcaatttctt cgctcagttt gactacctca cgtggttcga
    30181 tatagacggt gtttccacta gcagaaatat catgaacgac acctgcaatc ttattgcggt
    30241 aggtgttttt gactggtaaa acctgacggc catttctgct agcaacaatt ccttccgtca
    30301 acatctgccc tttttgcttg agcaagtctt gtaaaacatc gcgtacctga ctctcgctat
    30361 catgtatttt tcgacggatt cgcgccaatt cttcactggc aaaattttca atgaaacccg
    30421 catcattaaa ggcctgaaga tttccttgta attgcggaaa atcatgtaat ttctcaaacc
    30481 aaagggctaa ttcttccaag ctgacatttt ccagattggc gtaaaaactt tgaagttctc
    30541 ggctggcaag aagcacgcgt ttcaagagta ggaactcctc gatattgaga tccgctccca
    30601 tctccaacct cttgcagact cctgcaattt ccttagttga gagaatagta aaatgcggtt
    30661 gctcgacgaa aagagcctgc atttctttca tctcagcaaa agcctgtttg attttatctg
    30721 ctttggcagt cggagccagc tgtctcaatt gctccaagcc ctgctcggtc aacaaatgag
    30781 gctcaaacaa ggccttgacc ttatcgaact ctaatgtttc taatattttc ttattcatct
    30841 ttatttcctt gtctttgaat gtctaggtgt ggtagctttt tacattctga tagattcgag
    30901 tcaaatctgt aaacaaaggg ctaggaaaac tcctgccctt tttatccgat taaatttgtc
    30961 acccagattt gtttgagcca actggttgtt atcggtatgc tctggatgat gtgttttgcg
    31021 acgatactct tttcaagagg attttgtata actgccatag ggatggtcgc caagattgtc
    31081 aaggccattt gcaagacaaa taaggtcacc aacatggaca agatacctgc tgaaacttgg
    31141 aacaacttac cacccagttt tttgctagga atcaagtgta agagaagacc aagcaaacga
    31201 ccaatgctat agacaatccc aaatacaagc aagtagccga tacctgcata aaagacctta
    31261 tccagctgaa agagttgatc cgatgggaaa aagaaagtcc cctgaccttc ctgcgaattt
    31321 gcataaggga gcaataaatg gaattgctct ccaagcccct tataaaactg gccagccata
    31381 aaagccgatg ccatggctga aatcaggtaa taaacctgta agagcaggcc tctccgatag
    31441 ccgatataaa atccccaaac caagaccaat agaagaagga atgaaatcat aaggaatcct
    31501 caatcttgct ctgttcttgc ttacaagtca caagcttgtg acggagttct tctagctctt
    31561 gctccttatc gtcaaattca atctcacggc tgagctgagt tgataaacag ttgactgcca
    31621 acaaaagagc gattgtttca tcatctgcgc taggcatttg ttctttaatt gcttggtatt
    31681 tttctgtcgc aaccttagcg atttcctcca taaaaaggtt atcatgttcg cttgtcaagg
    31741 ttaacgtttt tttcccgaat gtaaatttga atcgatttag atttgccata aaattcacct
    31801 cacgatatta taccaaaatt cgctaatttt gtcagttttt acaaatttta ctgcttttgt
    31861 ggtacaatag aaactatggc aagtatcaca ctcacaccaa gcgaaaagga tattcaggct
    31921 tttcttgaac actatcaaac cagtctggct cctagcaaga atccctatat ccgctacttt
    31981 ttgaaactac ctcaagcaac ggtttctatc tatacttctg gaaaaatctt gcttcagggt
    32041 gaaggggctg aaaaatacgc cagtttcttt ggctatcaag ctgtagagca aaccagcgga
    32101 caaaatcttc ctttaattgg gacagatgag gtgggaaatg gttcctactt tggtgggctt
    32161 gcagttgtgg ctgcctttgt cacacctgac cagcacgact ttttacgaaa actcggtgtg
    32221 ggggattcta agactctgac cgaccaaaag atccgtcaga ttgctcctat tctcaaggaa
    32281 aaaattcagc accaggcact ccttctctca cccagcaagt acaacgaggt catcggagac
    32341 cgctacaatg ctgtttcggt taaggttgcc ctccataatc aggctatcta tctcctcctt
    32401 caaaaaggtg ttcagcctga gaaaattgtg attgatgcct ttaccagtgc taaaaattat
    32461 gacaagtact tggcacaaga ggccaatcgt ttcagcaatc ctatcagctt agaagaaaag
    32521 gctgagggca aatacttggc tgtcgcagtt tcttctgtca ttgcgcgtga tctctttctg
    32581 gaaaatcttg aaaatctggg acgagaactg ggttatcagc tcccaagtgg agctggaaca
    32641 gcttctgaca aggtggctag ccagattttg caagcctatg gtatgcaggg actcagcttc
    32701 tgcgccaaat tgcactttaa aaacactgaa aaagcgaaaa aacgcttaga aaggtaagtt
    32761 atgaattcat ttaaaaattt cttaaaagag tggggattgt tcctcctaat tctgtcatta
    32821 ctagctttaa gtcgtatctt tttttggagc aatgttcgcg tagaaggaca ttccatggat
    32881 ccgaccctag cggatggcga aattctcttc gttgtaaaac accttcctat tgaccgtttt
    32941 gatatcgtgg tggcccatga ggaagatggc aataaggaca tcgtcaagcg cgtgattgga
    33001 atgcctggcg acaccattcg ttacgaaaat gataaactct acatcaatga caaagaaacg
    33061 gacgagcctt atctagcaga ctatatcaaa cgcttcaagg atgacaaact ccaaagcact
    33121 tactcaggca agggctttga aggaaataaa ggaactttct ttagaagtat cgctcaaaaa
    33181 gcccaagcct tcacagttga tgtcaactac aacaccaact ttagctttac tgttccagaa
    33241 ggagaatacc ttctcctcgg agatgaccgc ttggtttcga gcgacagccg ccacgtaggt
    33301 accttcaaag caaaagatat cacaggggaa gctaaattcc gcttctggcc aatcacccgt
    33361 atcggaacat tttaagaaac ctaagaggcc gagaatcacc aatctcagcc tcttcttcta
    33421 tcgtgagaag tgattgttta ttgacaaata ctcaatgaaa atcaaagaac aaactaggaa
    33481 gctagccgta ggctgcttaa aacagtgttt tgaggttgca gatggaagct gacgtggttt
    33541 gaagatattt tcgaagagta taaaataaaa acaatcccaa ctctcaccta ttcatgcaaa
    33601 ggaattttat ggaagtttat ttttcaggaa ccattgaacg gattattttt gaaaatccca
    33661 gcaattttta tcgcatcctc ctcctagaaa tcgacgatac ggacgcagag gattttgatg
    33721 actttgaaat cattgtcaca ggaaccatgg ctgatgtaat tgagggcgaa gactatactt
    33781 tttgggggca aattgtccag cactccaagt atggagaaca actgcaaatc agtcgttatg
    33841 atcgcgcaaa accaactagt aagggcttgg tcaagtactt ttcaagtagc catttcaagg
    33901 ggattggtct caagacagct cagaaaatcg tggataccta tggcgaaaat accattgacg
    33961 aaattttgca acacccagaa aagttagaag gcatcgcagg actctctgcc aaaaatcgcg
    34021 aggctttcgt ctccactctc cgtctcaact acggtacgga gatggttttg gccaaactag
    34081 ccaactacgg cattcccaac aaactagcct ttcagattca agacttttac aaggaagaaa
    34141 cccttgatgt ggttgaaaat tatccctacc aactggttga ggatatcaag ggtttgggct
    34201 ttaccattgc tgaccaacta gctgaggaac taggcatcga aagtcaggct cctgaacgct
    34261 tccgcgccgg tctagttcac agtctttttc aggcctgtat ggaaacagga gacacctatg
    34321 ttgaagcacg ggatttgctg gaacaaaccc ttactctcct tgagtcttcc cgtcccgtgg
    34381 aactagaccc cagccaagtt gcccaagagc tctcctacct gatcgaagaa gacaaggttc
    34441 agcagattga taccaagatc tttgacaaca gcctcttttt cgctgaggaa ggcatccgca
    34501 gtcacttggt tcgtatcctt gaaaaaggaa aacagaagag tcatgattta gaaaccattc
    34561 aaaaacatat cactactatc gaggaagagc tgggaattga gtatgataac attcaaaaac
    34621 aggctatctg tgacgctatc cagaacaagg tctttatcct gacaggtggg cctggtactg
    34681 gtaagacaac tgttatcaat ggaatcatcg ctgtttatgc ccttttagaa ggacttgact
    34741 tcaggaagaa aaacaatctg cccattcttc ttgctgctcc aactggacga gccgctcgcc
    34801 gcatgaatga attgacaggt ttgcctagcg cgaccataca ccgccacttg ggaatgacag
    34861 gtgacgatga taccagtcat ctggaagatt acctagatgc tgactttatc atcgtggatg
    34921 aattctctat ggtggatact tggctggcca atcaactctt ctccaatatc tcttctaaca
    34981 gtaagatcct catcgtgggt gacagtgatc agctaccttc tgtcagtcct ggacaggttc
    35041 tagcggatct gcttcatatt cctttgattc ctcagactcg cttggaaaaa atttatcgac
    35101 aaagcgaaga atcaaccatc gtcaccctag ctagtcagat tcgacagggc atcttgccag
    35161 ctgatttcac ccaaaaaaaa gctgaccgtt cctactttga aattgctagt ggccatattc
    35221 ctgccactat tgaaaaaatc ttaggtgccg ccctcagaaa tagtattcct gcccgtgata
    35281 tccaagttct ggctcctatg taccgaggga cggcagggat tgatgctatc aatcagctca
    35341 tgcaagacct gctcaatccc ccacaaaaag atcaactcag ttttgaagct cctcagtgcc
    35401 actatcgtaa gggagacaag gtcattcatt tggttaacga tgctgaaatc aatgtcttta
    35461 atggggattt aggagctatc acagacctga ttcctggtaa atacaccgaa tcgaaacaag
    35521 acgagattgt cattgatttt gatggtaatg aagtctctta cccccgtaac gaatggtaca
    35581 agattcgctt ggcctatgcc atgagtattc ataagtctca gggaagtgag ttcccagttg
    35641 tcatcctacc gattactagt gctagccgac gtatgctgga gcgaaatctc atctacacag
    35701 ccattacacg tgccaaaagc aaactcatct tactaggcga attacaagcc ttcgactatg
    35761 ctacccaaca catcggaact gcccgaaaaa cctatctgat tgaacgcttc agtgatctac
    35821 tggagaatgt tgaagaaaag caacaagctg tctctgaaac agtcacatca agtgcctctg
    35881 aacaatccta catcctaacc gaagaaaact gggacagcat tccagccatg attgggatta
    35941 cagacacaga cctcaaagag atttttggaa aatagtgcat cagaaaaacc accaattcag
    36001 gtgggttttc tgtttattaa gattatttta ctgttgctgt gcttgtgatc aattcaacag
    36061 cttttttgat agtgatatca tgtttcaaca tgtcagctga aagcaagttt tgaacttgtg
    36121 caacttccat gttgtagtct gctgccaatt gctcaacttc tttttggatt tcttcttctg
    36181 aagcatcaaa tccttcagct ttggcaactg cttcgataac aaggttagtc ttagtacgtg
    36241 actcagcttc tgcttggtat tggttgtgaa ggtcttcttg agtagttcca gtgatttgga
    36301 agtacatgtc agggttgatc ccttgacgtt gcaaattccc aaggaattca tttactgaac
    36361 ggtgaacttc ttcatggatc atttcttctg gaagttctac gatttcagca ttttctacag
    36421 ctgtatcaat tgctgcacct tcaactgcat ctttgtaagc ttcttcttta gcagcagcca
    36481 attctttgcg gtatttttct ttcaagtcag caagtgtttc aacttcttca tcaatgtctt
    36541 ttgcaagttc atcgtcaaga gctggaactt ctttagcttt tacttcgtgg atagttgtca
    36601 cgaatttagc ttctttacct gcaaggtctt ctgcttggta gtcttctggg aatgttacga
    36661 taacatcaac ggtttcgcca gctgagtgac ctaccaattg gtcttcgaaa ccagggatga
    36721 attgacctga accaagtcca agtgagaagt tttcaccttt tccaccgtca aattcaacac
    36781 cgtcgataga accaacgaag tcgatcacaa cagtgtcgcc gttttcagca gcagcttcct
    36841 tgataaccaa ttcagccagg ttgttgcgtt cgcgttcgat acgctcttcg acatcagcgt
    36901 cagttacttc tttttctaca tcaactgata cttcaaggtt tttgtagtca cccaatttta
    36961 cttcaggttt tgtaacgact tcagcagtga taacccagtc ttgacctttt tccattgaag
    37021 ttacgtcaat ttttggttgg gcaaccactt caagaccagc ttcttttaca gctgcttcat
    37081 aagcgtttgg caaaagagcg ttcataacgt cttggtaaag tgattcttca ccaaattttt
    37141 ggtcgaagat aggacgtgga aggtgacctt tacggaaacc tggaacatta agagatttct
    37201 tcactgactt gaagacacgg tccaattctg gtttgatttg gtcttgagag atagtgaaag
    37261 tcaagacacc acggtttgtt tctttgtttt caaatgatac agacattctg tcatttctcc
    37321 ttaaaatttt ttaaatacag tctattataa cattttctat aaataaaatc tagactaatg
    37381 cagccaatta tagacaacaa gcctttttcc taactaaaac actcataaag tatagacaat
    37441 tttagactac tttggatatc ttttggttag tcattttcca aaactaattt atctaatttt
    37501 tcagttatat agta
//
LOCUS       DAVLVF010000013        36823 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVLVF010000013 DAVLVF010000000
VERSION     DAVLVF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36823
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.013"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            158..1387
                     /gene="arcA"
                     /locus_tag="VYN66_000603"
     CDS             158..1387
                     /gene="arcA"
                     /locus_tag="VYN66_000603"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV1494666.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1445..2461
                     /gene="argF"
                     /locus_tag="VYN66_000604"
     CDS             1445..2461
                     /gene="argF"
                     /locus_tag="VYN66_000604"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV1494667.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            2626..3573
                     /gene="arcC"
                     /locus_tag="VYN66_000605"
     CDS             2626..3573
                     /gene="arcC"
                     /locus_tag="VYN66_000605"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV1494668.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            3784..5295
                     /locus_tag="VYN66_000606"
     CDS             3784..5295
                     /locus_tag="VYN66_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV1494669.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            5317..6648
                     /locus_tag="VYN66_000607"
     CDS             5317..6648
                     /locus_tag="VYN66_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV1494670.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(7053..7343)
                     /locus_tag="VYN66_000608"
     CDS             complement(7053..7343)
                     /locus_tag="VYN66_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494671.1"
                     /translation="MGALGYYEGFVPYVSNQYQNQAEEEYKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(7331..7582)
                     /locus_tag="VYN66_000609"
     CDS             complement(7331..7582)
                     /locus_tag="VYN66_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1494672.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            7786..7863
                     /locus_tag="VYN66_000610"
                     /pseudo
     CDS             7786..7863
                     /locus_tag="VYN66_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(7981..8865)
                     /locus_tag="VYN66_000611"
     CDS             complement(7981..8865)
                     /locus_tag="VYN66_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1494673.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(9010..10161)
                     /locus_tag="VYN66_000612"
     CDS             complement(9010..10161)
                     /locus_tag="VYN66_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV1494674.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(10307..12073)
                     /locus_tag="VYN66_000613"
     CDS             complement(10307..12073)
                     /locus_tag="VYN66_000613"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV1494675.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGYTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELDESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDSGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(12130..15246)
                     /locus_tag="VYN66_000614"
     CDS             complement(12130..15246)
                     /locus_tag="VYN66_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1494676.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            complement(15256..17550)
                     /locus_tag="VYN66_000615"
     CDS             complement(15256..17550)
                     /locus_tag="VYN66_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1494677.1"
                     /translation="MKLWYKKAAANWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELCIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKGCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPRTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQDREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            complement(17609..18409)
                     /locus_tag="VYN66_000616"
     CDS             complement(17609..18409)
                     /locus_tag="VYN66_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1494678.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            complement(18406..19179)
                     /locus_tag="VYN66_000617"
     CDS             complement(18406..19179)
                     /locus_tag="VYN66_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1494679.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            complement(19204..19674)
                     /locus_tag="VYN66_000618"
     CDS             complement(19204..19674)
                     /locus_tag="VYN66_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1494680.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            complement(19665..20096)
                     /locus_tag="VYN66_000619"
     CDS             complement(19665..20096)
                     /locus_tag="VYN66_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1494681.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            complement(20089..20529)
                     /locus_tag="VYN66_000620"
     CDS             complement(20089..20529)
                     /locus_tag="VYN66_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEV1494682.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            complement(20541..21179)
                     /locus_tag="VYN66_000621"
     CDS             complement(20541..21179)
                     /locus_tag="VYN66_000621"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEV1494683.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            complement(21294..22697)
                     /locus_tag="VYN66_000622"
     CDS             complement(21294..22697)
                     /locus_tag="VYN66_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV1494684.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGEITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            22873..23646
                     /locus_tag="VYN66_000623"
     CDS             22873..23646
                     /locus_tag="VYN66_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1494685.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            complement(24817..26322)
                     /gene="adcA"
                     /locus_tag="VYN66_000624"
     CDS             complement(24817..26322)
                     /gene="adcA"
                     /locus_tag="VYN66_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV1494686.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYTEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(26332..27138)
                     /locus_tag="VYN66_000625"
     CDS             complement(26332..27138)
                     /locus_tag="VYN66_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1494687.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAVVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            complement(27131..27835)
                     /locus_tag="VYN66_000626"
     CDS             complement(27131..27835)
                     /locus_tag="VYN66_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1494688.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            complement(27835..28275)
                     /gene="adcR"
                     /locus_tag="VYN66_000627"
     CDS             complement(27835..28275)
                     /gene="adcR"
                     /locus_tag="VYN66_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV1494689.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            complement(28430..29698)
                     /gene="dltD"
                     /locus_tag="VYN66_000628"
     CDS             complement(28430..29698)
                     /gene="dltD"
                     /locus_tag="VYN66_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV1494690.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLQQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(29691..29930)
                     /gene="dltC"
                     /locus_tag="VYN66_000629"
     CDS             complement(29691..29930)
                     /gene="dltC"
                     /locus_tag="VYN66_000629"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV1494691.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(29944..31188)
                     /gene="dltB"
                     /locus_tag="VYN66_000630"
     CDS             complement(29944..31188)
                     /gene="dltB"
                     /locus_tag="VYN66_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV1494692.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQTIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(31185..32735)
                     /gene="dltA"
                     /locus_tag="VYN66_000631"
     CDS             complement(31185..32735)
                     /gene="dltA"
                     /locus_tag="VYN66_000631"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV1494693.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNAHIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFENLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(32756..32887)
                     /locus_tag="VYN66_000632"
     CDS             complement(32756..32887)
                     /locus_tag="VYN66_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV1494694.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(33201..34379)
                     /locus_tag="VYN66_000633"
     CDS             complement(33201..34379)
                     /locus_tag="VYN66_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1494695.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            complement(34835..36112)
                     /gene="ltrA"
                     /locus_tag="VYN66_000634"
     CDS             complement(34835..36112)
                     /gene="ltrA"
                     /locus_tag="VYN66_000634"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEV1494696.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLKVEIPKPDGGIRQLGISTVMDRMIQQAIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVVSEAAAKRMMYSVSRFIEKRLGLKANMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     SIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
BASE COUNT        11232 a         7765 c         6361 g        11465 t
ORIGIN      
        1 tgttgtgtat aataacataa aattgtgcta aatatcataa tttatattat tttccgagaa
       61 attatagcca ttgacattac ctaggaattg cgttataaca tacatgtaag cggtacccaa
      121 aatggtgcca agtcaaaatt tttaaggagg aaaatacatg tcttcacatc caattcaggt
      181 cttctcagaa attgggaaac tgaaaaaagt tatgttgcac cgtccaggca aggagttaga
      241 aaacttgttg ccggactatc ttgaaaggct tctttttgat gatattcctt tcttggaaga
      301 tgctcaaaaa gaacatgatg catttgccca agctcttcgc gatgaaggaa ttgaggttct
      361 ctacctagaa caactcgctg ctgaatcatt gacctctcca gaaatccgcg atcaatttat
      421 cgaggaatac ttagacgaag ccaacatccg tgatcgtcaa actaaggttg ctattcgtga
      481 attgcttcac ggcatcaagg acaaccaaga attggttgaa aaaacaatgg ctgggattca
      541 aaaagttgaa ttgccagaaa ttcctgacga agctaaagat ctaactgact tagttgaatc
      601 agattatcca tttgcaattg acccgatgcc aaacctctat ttcactcgcg acccatttgc
      661 aacaattgga aacgccgtat cgcttaacca catgtttgca gacactcgta accgtgaaac
      721 actctacggt aagtatatct tcaaatacca cccaatctat ggcggaaaag tggatttggt
      781 ctacaaccgt gaagaagata cgcgtatcga aggtggagac gagctagttc tttctaaaga
      841 cgtccttgca gtaggtatct ctcaacgtac agacgcagct tctatcgaaa aacttttggt
      901 caacatcttc aagaaaaatg ttggcttcaa gaaagttttg gcctttgaat ttgctaacaa
      961 ccgtaaattc atgcacttgg atactgtctt cactatggta gactatgaca agttcactat
     1021 tcacccagaa atcgaaggcg accttcacgt ttactcagtt acttacgaaa acgaaaaact
     1081 taaaattgtt gaagagaaag gtgacttagc tgaacttctt gctcaaaacc ttggtgtaga
     1141 aaaagttcat ttgattcgtt gcggtggtgg caatatcgta gcagctgcgc gtgaacaatg
     1201 gaacgacggt tctaacactt tgaccatcgc acctggtgtg gtagttgttt atgaccgcaa
     1261 taccgtgacc aataagattt tggaagaata cgggcttcgc ttgattaaga ttcgcggaag
     1321 tgaattggtt cggggccgtg gtggacctcg ttgtatgtct atgccatttg aacgtgaaga
     1381 agtgtaatcg ctgttcggta ttcgtcaata gaaaatgtaa aaaatagaaa gaggaaataa
     1441 taaaatgaca aattcagtat tccaaggacg cagcttctta gcagaaaaag actttacccg
     1501 tgcagagtta gaatacctta ttggtctttc agctcacttg aaagatttga aaaaacgcaa
     1561 tattcaacac cactaccttg ctggcaagaa tatcgctctc ctatttgaaa aaacatctac
     1621 tcgtactcgt gcagccttta caactgcggc tatcgacctt ggtgctcacc cagaatacct
     1681 cggagcaaat gatattcagt tgggtaaaaa agaatctact gaagatactg ctaaagtatt
     1741 gggacgtatg tttgacggga ttgaattccg cggattcagc caacgtatgg ttgaagaatt
     1801 ggcagaattc tcaggcgttc cagtatggaa cggtctaact gacgaatggc acccaactca
     1861 aatgctcgct gactacttga ctgttcaaga aaacttcggt cgcttggaag gcttgacatt
     1921 ggtatactgt ggtgatggac gtaacaatgt tgccaacagc ttgctcgtaa caggtgctat
     1981 ccttggtgtc aatgttcaca tcttctcacc aaaagaactc ttcccagaaa aagaaatcgt
     2041 tgaattggca gaaggatttg ctaaagaaag tggcgcacat gttctcatca ctgaagatgc
     2101 tgatgaagca gttaaagatg cagacgttct ttacacagac gtttgggtat caatgggtga
     2161 agaagacaaa ttcgcagaac gtgtagctct tcttaaacct taccaagtca atatggactt
     2221 agttaaaaaa gcaggcaatg aaaacttgat cttcctacac tgcttgccag cattccacga
     2281 tactcacact gtttatggta aagacgttgc tgaaaaattt ggtgtagaag aaatggaagt
     2341 aacagacgaa gtcttccgca gcaagtacgc tcgccacttc gatcaagcag aaaaccgtat
     2401 gcacactatc aaagctgtta tggctgctac acttggtaac ctttatattc ctaaagtata
     2461 attttagata ataaaccgtc taccaacagc tatgagggct gcgactaata gctttagtcc
     2521 ggtcctcttt tatataatgg taatctatta tttcttataa aatatgtgaa aaatcattaa
     2581 attgaaatct aaacgcattc tattgagtgt gataaaggag aatttatggc aaatcgtaaa
     2641 attgtagtag ctttgggagg aaatgcgatt ctttcttctg acccatcagc aaaggctcaa
     2701 caagaagctt tagttgaaac agctaagcat cttgtaaaat tgattaaaaa tggagatgat
     2761 ctgattatca ctcacggtaa tggacctcaa gttgggaatc tcttgctcca acatttggca
     2821 tcagactctg aaaagaaccc tgccttccca ctcgactcac ttgtcgctat gacagaaggt
     2881 agcatcggtt tctggttgaa aaatgctttg caaaatgctc tcttggatga aggcatcgaa
     2941 aaaaatgttg cctctgttgt aacgcaagtt gtcgtagata aaaatgatcc agcttttgtt
     3001 aacttgagta aaccaatcgg tcctttctat tcagaagaag aagcaaaagc agaagccgaa
     3061 aagagcggag cgactttcaa ggaagatgct ggccgtggct ggcgtaaggt cgttgcctca
     3121 ccaaaacctg ttgacatcaa agaaattgaa accatccgta ctcttttaaa taatggtcaa
     3181 gtcgtcgtag ctgcaggtgg tggcggtatt cccgtcgtca aagaaaacaa tggacatttg
     3241 actggtgtcg aagcggttat tgataaagac ttcgcttccc aacgtttggc agaattggtt
     3301 gatgcagacc tcttcatcgt tttgacaggt gtagattatg tatttgttaa ctacaacaag
     3361 ccaaaccagg aaaaattgga acatgtgaat gttgcccagc tggaagaata tatcaaacaa
     3421 gatcagtttg caccaggtag catgcttcca aaagtagaag cagctatcgc ttttgtcaat
     3481 ggtcgtccag aaggaaaagc agttattact tcccttgaaa atctaggcgc cttgattgaa
     3541 tctgaaagcg gaacaattat tgaaaaagga taagttgttt tactaataag atgtattcta
     3601 tttctagtat ctttatatca aattagaaat tattcttgaa aacatgtaca atatttcaaa
     3661 agatattagt tttttagact ttaatatggt aaaacaaata taaatagaaa gcgttttctt
     3721 aaatgtttat ttaagaaagt agttggtttt ttacactttg ttagacatca ggaggaaaaa
     3781 caaatgagtg aaaaagctaa aaaagggttt aagatgcctt catcttacac cgtattattg
     3841 ataatcattg ctattatggc agtgctaact tggtttatcc ctgcgggggc ctttatagaa
     3901 ggtatttacg aggctcagcc tcaaaatcca caagggattt gggatgtcct catggcaccg
     3961 attcgggcta tgctaggtac tcatccagag gaaggttcgc tcattaaaga aacgagcgca
     4021 gcgattgatg tagccttctt catccttatg gttggtggtt tccttggcat tgtcaacaaa
     4081 actggtgctc ttgacgtagg gattgcctct atcgtgaaga agtataaggg ccgcgaaaaa
     4141 atgttaattt tggtactgat gcctttgttt gccctcggtg gtacaactta tggtatgggg
     4201 gaagaaacaa tggccttcta tccactcctt gtgccagtta tgatggccgt tggttttgat
     4261 agcctgactg gtgttgcaat tattttgctc ggttctcaaa tcggctgttt ggcatctact
     4321 ctgaatccat ttgcgacagg tattgcttca gcgactgcgg gagttggtac aggggacggt
     4381 atcgtacttc gtctgatttt ctgggttacc ttgactgctc ttagtacttg gtttgtttac
     4441 cgttatgcgg ataagattca aaaagatccg actaagtcac tggtttatag tactcgcaaa
     4501 gaagatttga aacactttaa cgtagaagaa tcttcatctg tagaatctac acttagcagc
     4561 aaacaaaaat cagttctctt cttatttgtg ttgacattca tcttgatggt attgagcttc
     4621 attccatgga cagaccttgg cgttaccatt tttgatgact ttaatgcttg gttgactggt
     4681 cttccagtta ttggtaatat tgtcggttca tctacttctg cactaggtac ttggtacttc
     4741 ccagaaggcg ccatgctctt tgcctttatg ggtatcctga ttggtgttat ttatggtctt
     4801 aaagaagata agattatctc ttccttcatg aatggtgctg ctgacttgct cagtgttgcc
     4861 ttgatcgtag cgattgctcg tggtattcaa gttatcatga acgacggtat gattaccgat
     4921 acaatcctca actggggtaa agaaggcttg agcggtctat cttcacaagt ctttatcgtt
     4981 gtaacttata tcttctatct acctatgtca ttcttgatcc catcttcatc tggtcttgcc
     5041 agcgcaacta tgggtatcat ggctccactt ggagaatttg taaatgtccg tcctagcttg
     5101 attatcactg cttaccaatc tgcttcaggt gtcttgaact tgattgcacc aacatctggt
     5161 attgtgatgg gagctcttgc acttggacgt atcaacattg gtacttggtg gaaattcatg
     5221 ggcaaactcg tagtcgctat tattgtagtg accatcgccc ttcttctcct tggaaccttc
     5281 cttccattcc tataaaatag tgagtgaggt gattccatga aaatagatat aacaaatcaa
     5341 gttaaagatg aatttcttat atcattaaaa accttgattt cctatccttc agtactcaat
     5401 gaaggagaaa atggaacacc ttttggacaa gcaatccaag atgtcctaga aaaaacttta
     5461 gagatttgtc gagacatagg tttcactacc tatcttgacc ctaaaggtta ttacggatat
     5521 gcagaaatcg gtcagggagc agagcttctg gccattctct gtcatttgga tgttgttcca
     5581 tcaggtgatg aagcagattg gcagacaccg ccatttgaag caactatcaa agacggctgg
     5641 gtattcggac gtggtgtcca agatgataaa ggcccttcgc tcgcagctct ctatgcagta
     5701 aaaagcttgc tggaccaagg tattcagttc aaaaagcgcg tacgctttat ctttggtacc
     5761 gatgaggaaa ccctctggcg ctgcatggca cgctacaata ccatcgaaga acaggccagt
     5821 atgggctttg cacctgactc atcttttcct ctgacctatg ctgaaaaagg gcttctacag
     5881 gtcaaacttc atggcccggg atcggatcaa ctagagcttg aagtaggagg cgcctttaac
     5941 gttgtaccag acaaggccaa ctaccaaggt ctcctctatg aacaggtttg taacggtctc
     6001 aaagaagctg gttatgatta ccaaaccact gaacaaaccg taacggttct cggagtgcca
     6061 aagcatgcta aggatgctag tcaaggtatc aatgctgtca tccgactagc taccattctt
     6121 gctcctctcc aagaacaccc tgctctcagt tttcttgcaa cacaagcagg tcaagacggc
     6181 acaggaagac aaatctttgg tgatatagca gatgaacctt ctggtcacct atcctttaat
     6241 gtcgcaggtc tcatgatcaa tcatgaacgt tctgaaatcc gtattgacat tcggactcct
     6301 gtcttagctg acaaggaaga actagtagag ttgcttacaa gatgtgcaca aaactaccaa
     6361 ctccgctacg aagagtttga ctatctagcg cctctatacg tcgcaaaaga cagtaaactc
     6421 gttagcacac tgatgcaaat ctaccaagaa aagactggcg ataacagtcc tgctatttca
     6481 tccggtggtg ccacttttgc tcgcaccatg ccaaattgtg tagccttcgg cgccttattc
     6541 ccaggagcga agcagacaga acatcaggca aatgaatgtg ccgttctaga agatttgtac
     6601 cgtgctatgg atatttatgc cgaagccgtc tatcgacttg caacttaatc aggcaactgt
     6661 ttctacaaaa aaaatcgacc gattaatgaa ctgcacccca aaagttagac agaataaatc
     6721 taacttttgg ggtgttttat tatgaaattg agttatgaag ataaagttca gatctatgaa
     6781 ctaagaaagc aaggacaaag cttcaaacag ctttcaaaaa gatttggtgt ggatgtttct
     6841 ggtctaaagt catctgaatc tttgagatga gctttataaa ttgctttttt cagtttttgc
     6901 actggtattt cgataaactc aaacttttta gccgtggtat tgcctgattt tatagtatat
     6961 tgaaactaga atagtacacc tctacttcta aaacattttt agaaatcgat ttgactgtcc
     7021 tgatcgattt gtcctgttct tatttcattt tactatattt gagccacttc gtctttaacg
     7081 gctttattca taagctcttg taatttttct ttactatcaa ttacttctga ttttccgttg
     7141 taatttattg taataggttt taacttacct aatttctcga cacgctcatt aatttgatct
     7201 tttttgaagg ctgcatatgt ttttcctaag attttttcga aaatatattt atcagatagc
     7261 ggtttgtatt cttcttcagc ttggttttgg tattgatttg aaacataagg aacaaatcct
     7321 tcatagtaac ctaatgctcc cataagttca aaagcttgtt ttctaattca aaccattgca
     7381 actcagattt cagcttttca gataaatcct gctcatccaa ataatgactt gaaattagtg
     7441 ctgaactcgt ttctgtatcc tgtacaggct gagcacccat accagcaaaa aataaactcg
     7501 ttcctagcaa gaccgaacaa gctcctattg catatttcct caaagaaaaa cgctgctttc
     7561 tctcaaattg aaattctttc atcccatctc ccatcattca ttattactgt atattttgta
     7621 tatcagaaat agtttgtatt cacaaatctt tctagttatt cccttatcat tcctaattaa
     7681 gggagataac atacaataat ttttagttaa atgtatatcg atgttttttg tttttcttaa
     7741 taaacgcaat acaaaaagag cctgttgcca agctctttat actcaatgaa aatcaaagag
     7801 caaactagga aactagctac aggttgctca aaacaccgtt ttgaggtagt agataagact
     7861 gacgaagtca gctcaaaaca ctgttttgag gttgcagata gaactgacga agtcagtaac
     7921 atctatacgg caagacgacg ctgacgtagt ttgaagagat tttcgaagag tattagtcta
     7981 ttatttcttc tcagcgcgaa gggctgacaa gatttgtgta cggatatcat ccacaccatt
     8041 tggagtattt ggtaaaaaga tggtttgatt tcctttagag gcaaaggtat tcaaggtatc
     8101 caaatactgg ttggtcaaga ggatagacat gatttgttct tctgtcatgc caacattggc
     8161 ttccttgagt tcggtgatag actctgccaa tccatccaca atcgccttac gttgttgggc
     8221 aatccccaca ccatgaaggc ggtctttttc tgcttcggct tcagctgcag tgacaatttt
     8281 aatcttgtca gcttccgcca attcttgtgc tgcgacccgc ttacgttgcg ccgcattgat
     8341 ttcattcata gattgcttaa cttctgcatc tggttcgacc ttggtaatca aggttttcac
     8401 gataatgtag ccgtaagtgg tcatttcttc tgctacttgg tgttgaacct caagggcaat
     8461 ctcatctttt ttctcaaaca attcatccaa ggttaatttt ggaacagaag agcgaagagc
     8521 atcttcgata taagatttaa tctgagattc tggacgtatg agtttatagt aagcatctgt
     8581 cacgctctgc tcgttgacac ggtactgagt cgctacattc atcataacga acacattgtc
     8641 cttggtctta gtctcaacca caatatcact ttgcaacaag cgcaactgaa tccgtgctgc
     8701 aatcgagtca atcccaaaag gcaagcgaat atgaataccg ctattagcaa ccttttggta
     8761 tttcccaaag cgttcaataa tcgccaccga ctgctgacga accacataaa ctgtactcag
     8821 tgtgactatc accaatagga gcacacaaac aatcagaaaa atcatgaaaa atattgccat
     8881 aatggaacct ccacaagtat ttttctagta ttatagcaca tttaaagaag gctgtgccgt
     8941 ttttactgcg atttttcctg aaatgtcaat aattagaggt gaattgtcct attgtcgtcc
     9001 aatctcttgc taaaataact ctttataaaa ggcaatcgtt tcttctaagg ttggcataaa
     9061 tggatttcct ggtgcgcagg catcaatcaa ggcattctta gaaaggtatt caaagtcgaa
     9121 atctttttct tcaataccaa gttcagtcag tttcttagga atacctactg tctcagaaag
     9181 cttctcaatc tcagcaatcg cataatcggc acattcttga tctgatttac cttctacatg
     9241 aagtcccaag gctttggcaa cattgcggaa agcttctggt acacgtttag cattttcacg
     9301 ttctataact ggtagcaaca tggcacagca cacgccatgt ggcaaattat atactgcacc
     9361 gagttggtga gccattgagt gaacatagcc aagaccagca ttattaaagc tcataccacc
     9421 aaggaagatg gcattgacca taccttcacg cgcttcaata tcatgtccat tttctacagc
     9481 acgaggaaga tattccttga taagctcaat agcaccaata gacagtttct tggttacatc
     9541 ataagcacca ggtgttacca aagcttcaac agcatgagtc agagcatcca taccagtagc
     9601 agcagtcagg cctttaggtt tggaaagcat gagttcaggg tcattgacag aaatcaaggc
     9661 aaggctattc ttgtcaacca ttaccatctt aaccttgcgt tcttcatcag taatcacata
     9721 gttaatggta atttctgctg atgtacctgc cgttgtattg atagccacaa ctggcaaacc
     9781 ttttttagca gacttatgaa gcccttcata atcctgtggt tttccaccat ttgtagccat
     9841 gatagaaata caactagctg catcctgtgg agaacccccg ccaagactga tgataaagtc
     9901 acagccatgt tcttgcaaag cagctacccc atctgtgaca tttttacaag tagggtttgg
     9961 ctctacatcg ctaaagatga catattcgat tccttctgta tccagtggtt ttaaagtctt
    10021 aggtaaaata tcactgcctt cgatgtactt atctgtcacc aaaagtgcct ttttatagcc
    10081 aagttccttg atataaggac ccacttcatt tacaacacct tttccaataa ggttgactgc
    10141 cggaacataa aatatagcca tataattttc ctcctgcgcc tccgcgctat ctatttaaca
    10201 tcattatatt actttttgta ttcgttttca attatttgtg aattgtttaa caaatctttt
    10261 taaaaggata ggagggacgt aagttcacct cctatataaa caagttttat ttatgtagtg
    10321 gtcccaacaa ctgacatgca cgatagtctg ctgattctag atcttctgtt ccaaataagg
    10381 accaattttt aggtctaaag atatcttcct caggtacatt atgcatattg acaggaattc
    10441 tcaacataga agccaaggta atcaagtctg ctccaatgtg tccatatgtt atggctccgt
    10501 gattagctcc ccaattattc atgacgtcat agacagactt gaaagcacct tttcctgtca
    10561 aacgtggagc aaaccaagta gttggccatc ctgaatctgt acgattatct aaagtatggt
    10621 gaacatcttc aggaagttca agtgtgtaac cttctgcaat ttgtagcact ggaccaaccc
    10681 cttttagaag attgagacgt accattgtta ctggcatatc ccccttcgtc aagaaacgag
    10741 ttgagaatcc tcctccacgg aagtactcgc ggtttgctgg tgggaagtct gtattttcaa
    10801 gcatagcctg tacttcactt tcatccaact cccagaatgg tttcataaca ggtttgccat
    10861 ctcgagtagc ttgacctgta ccatccaatg tacaagaacc agagttgatt agatgtaaga
    10921 agccagctgc agcacgaccc tctaaagtat atcctgttac acgttcaaca gcctctggac
    10981 tccaataagt acgcacatca gcaaagattt gtggagtatt tgttagtaga taattaaaga
    11041 gcatagacac accatttagt gaatcattct ctgtcgcaaa tacaaatggt tttcgaatac
    11101 cattccagtc aaactgagta ttgaggaaag tttccataaa atctccattt ggaaaatggt
    11161 ctgtccactg acgttgacct tggaaaccag ctactaaagc atggtgacca actgcttctt
    11221 cctcaaaacc aagttcagca agtcttgggt taccaaccat taagtcacgt ccaatcatga
    11281 acatcttaat aacaaattcc cattgttttt ctttctcttc tttagaaaga atcaaatcct
    11341 cacgatttcg gtcaactcct tctttgatat gttcttttac ccaaaccata gcacgttcaa
    11401 actcttcagg gtcgtaaata ccacggtcca tacggcgtgt gaactccgtc atatctaccg
    11461 attcatttcg cattcctaag tattcttgga agaaatccgg atttacaata gaaccaccaa
    11521 tccccatcga aacactaccc attgataggt aagcagtgtc tctcatcaag ccagttgcaa
    11581 gagctgcacg cgcataacgt aaaagttttt ctttgacatc ttctggaata tctgtatcat
    11641 tagcttcctg aacatctcta ccataaatcc caaaggctgg aatccctttt tgagcatgtg
    11701 aagctagtac agctgcaaga tagacagctc ctgggcgttc tgtcccatta aatccccaaa
    11761 tagcatgagg aatatctgga gacatatcca tagtttcact accataacac cagcatggtg
    11821 taactgtaat tgttgcgcaa acatttgatt ttttaaacaa ctcatgggaa gctgcagcct
    11881 ctggaacacg accaatggta gatggagaaa tcacacattc cacaggttcc ccatctggat
    11941 atttcaatgt gcttgaaatc aaatctgcca cacttttagc catgttcatt gtttgtactt
    12001 caagtgattc gcgtacacct tgacgacgac catcaatagt cggacgaata ccaatacgtg
    12061 gatgttgaat catactattt tcctcttatt tttctgtttc tttcatacta ccgatgataa
    12121 tttttaaaat tacttataag tataaacctc tacctctgcc aaacttaaag gaattcctgc
    12181 gtttctcaaa gaaattctaa ccttttttcc cttcagccct tttaagtcta tggatagatt
    12241 attgctttct aaactgtcaa tatgttgttt aaaaacttca tgattatttg aatcatatat
    12301 aataatatca aaatttgata atctctgaat ttcagcatct gttctattat aaataattaa
    12361 cttttctaat tcttcggttt gtgctaaatc tatctcccac catgaaggag aatctccttt
    12421 ggtatgagtc actgaatgat gaccataatc tccgttttta tttccatcaa ctgctagacg
    12481 agaaaaacca ttgtaatccg ttgaactctg tcgagtttct ttggttaatg caatattaga
    12541 aagtttcttc ggagttctct ttgaaccata gacttcaact tccgctaaac ttaacggaat
    12601 agctgctgat tttaattcta ttttgactag cttagcccct acagaaggta cttttaaaga
    12661 taacaaacca tcaactactt tatcaaaatg tcttctaaaa acttcttctc ctgatgaaga
    12721 tagaaaaata acatcaaaat tagataaacg ctgaggttcg gcatctgttc tattataaat
    12781 atcaacttta gaaaccgtaa actcttctcc cagatcgacc tgccaccaag cgttagaatc
    12841 tgccttagta tgagtcaccg aatgatgtcc gtaatctcca tctttattac catcaactgc
    12901 taaagcagct acaccctcat aattggttga actttgagta gctaccttat ttgtagagac
    12961 tactttatct tctgttattt tatctatatc ctcttcagat tgcttaccat ctgattctct
    13021 aaaaacctct acttctgcta aactcaaagg cactccactc gttagtaatt taactttaat
    13081 ataccttgct cctttttctt tgaaatctag actaacagat tcacccgaca aattattaac
    13141 atgtttctta gcaacttcgt ttctattatt gtcatataga atcacatcaa aattagatag
    13201 acgttgagtc tcagcatctg tgcgattata aattttaaca agcccaactt tatccatttt
    13261 tttcaaatcg acttcccacc aagagggatt atctgcccta gtgtgtgtta ccgaaccaga
    13321 gttaaaatta ccatttctgt taccatccac agctctatta gggtctccat tgtaatctac
    13381 agaactttgt gttgttggtt ttgcatatgc tatattcaaa ttaccatcat taaatttatc
    13441 tggtgttgga aaagggactg ggcctgtccc ttcagtattt atagacatct ctaccattcc
    13501 attatgatta accgtaatgg tataaacatg ggtattctca tcccaatttt ctgtataagt
    13561 ataatgattt ttatcgccac ttatatttat ctgaggttta tgaccagtat gcccttttaa
    13621 tgtaagtgtt gttgtcctaa agtcattatc tacaggattg atggaataat ttttgcttat
    13681 ccagttcgct aactctaatt cttctttctt ccaacttttt gatgcatcaa aatttcctga
    13741 taatgcccac atagctgtct tatctgtcct gtaattgttc aataaaatat gtaaattatt
    13801 tggattttct ttaacaacag cgtaagtatg tggcgtcaaa tctaacgata acgactttgt
    13861 attattagta tacataggca acattacttg ctgattttta ttgatattag cattactatt
    13921 ataaatatac caggaattac ctacacgctg agcataccca tctccttcat aaagttttgg
    13981 ataaagcgag tttaaatagt tgactttact agacaattcc tcactatttt tagtcaaaat
    14041 ttttgcatta gggaatatag atgaaatctt ttccttatca attttctcat gtattacagg
    14101 aagaatatga tatctcccat tattatataa aggcattgtt tcatcattcg aataaagtcc
    14161 ttgataaaat ccgtttaatg aactaatcct accttctcca ttccaaaata cagcttttgt
    14221 tctatttaca acttcttcct tacttggagc tggattttgt atagcatgtc taaagaaagg
    14281 aataatacct ttagtaaatg ctggagttgg tacatcattt gtcataaatg tatacgcggc
    14341 acattcaaaa ttataaactg tgcctccccc agtatataca ttcatcattt ccatagcaat
    14401 cattgattct ggttccgatg cataggatct catatcccta gctcttccag tttcaaatgt
    14461 gtttgtataa tgtttttccc accatttcca tgtatctgtt gatgagcccc agttatcaca
    14521 taagccactc aaccaaaatc cactaacgat agaatctgta cccgcatcat ctcttattgg
    14581 cgtattttta gttgccaaca ccaaattttt atgatatttt tgactcgctt caaagaatgt
    14641 cggatcattc ataatagttt cccagaacca tttttcatga tcatgccaga taaaatgcgc
    14701 tccatatttg gcacaaactt ccaaatattt agcactatgc ggagctaact ggttattgta
    14761 aatccaataa ttctcaatat ttaaaacacc ttttaacaca ctatactttt ggaattgttc
    14821 atctaaccac tctggaggaa ctgtattacg ctctccagcc gacataataa ccaagaaaac
    14881 tggaatgttt agactttgag cttcctccaa catcttgaca taccattctc tcaattcttt
    14941 cgtatctcgt ggaatacaac ttgttggttt acagactttt gcaggatgaa gttcaatcgc
    15001 tgcatatggc tttacatctt caggaatagc ttcccatgct cccttcaatg tgttccccca
    15061 ccataatccg ttaccattat cattgccata caacggagaa agcaacaatg gcgattcatt
    15121 attaatcgtc gttctcattt gaacacgatt atcagcctta actcctgcta acaatccaaa
    15181 acataacatc ccaatacata caaacaatat tgtgattaat tttcttttta ttttttcttt
    15241 attcatgact tatacctaaa aatttaactc aataattttt tctgaattaa ataccaattc
    15301 aatattttgt acatcagtat ttttgccata tattcttact cttacttttt gatctttgtt
    15361 tcctccttcc aattttagga atgttatatc cccatttttc caagcaaacg ataccttata
    15421 tcctcctctt actctgaaac ctttcacttc tccttctgac caagcagaag gtaaagctgg
    15481 aattagtgat aaccaattat gatggctctg tactaataat tcacaaattc cactcaccaa
    15541 acctaaatta ccatcaattt gaaatggtgg atggtcaaga aataaattgc caagagtcgc
    15601 attatttaac aaaccattaa tctggttata agcaggttca ccttgatata gtctcgcaaa
    15661 aaaatgaatc agccatgcag cactccaacc tgtttgtgta ctagcatgca aaccacttac
    15721 taaccaatta ttaatcgctt gctccctatc ctgtgaagat aaaaagttag cgtttgataa
    15781 tctcctattg atagtgattt tagctgcttc tgctaattcc ggagttttat gaatatcaat
    15841 ctcattataa ggataaagcc caaatagagg tgaaatgtgt ctatgaccag gctctacttc
    15901 ttcataatcc tctagccatt cttgtatttg cccattacta cctatttttg ttctaggtaa
    15961 tttctttttt aactccttca cacgactaat aaaatccgaa ttgtctccta attgttttgc
    16021 aatgccaatg catgaatcac aaaaatatct tagaatttga ttatcaattg tagatgatag
    16081 acaagcattt ccttcaatac cattttttaa gcgatattta ttttccggtg agacacttgg
    16141 acctgtcatc aagtagccat ccacctcaaa taaataatct tcaaagaaaa gaaatgcttc
    16201 ttttatcatt tcaaaatgtt ccgtaagaat acgctcatct tggaaatata aatagtgttc
    16261 ccaaatatga gtacataacc atggaatagt taatacccaa attgcagccc ccatggcatg
    16321 agattgggga gccgtatcgc caaaaccatc cgtattatga tgtgctgtaa aacctctagc
    16381 tccatacatt ttcttagcgg ttagtcttcc cggttctctc attctttcga gcatatcaaa
    16441 taatggatat tctacttctg gtaaatcaca tggacctacc atccaataat tcatttgagt
    16501 attaatatta atcgtatatt tagaacccca aattggattt aattcatcac accatattcc
    16561 ttgaagattg gcaggtaaac cattcggttg actagacgat attaacagat atcttccata
    16621 atgaaataac aagttagtca agtagttact atacttttta gtgttttcaa gaagtagatt
    16681 cgttggaatg ctaagacaac ctttactata gtctagttta aaatcaactc tattaaattg
    16741 ctcctgatat ttttttacat gttcatcttt ttctgtaaag taatcaatac tactaaattc
    16801 tccctgaaga gaagaaatat ctatatttcc ccaataatcc gtcattgatt tgagataaag
    16861 aaatacctct gtagcattcc gaataactat tgtctctccc aatacactta cttcaccatc
    16921 cgtaacctta gaatgacata ctactttaaa ctgaacacct tttctacctc cagcagaggc
    16981 cgacattaaa attgtacttg aatccagttt agatacttcg tcattaaacc gtttatttct
    17041 acccaaatta atgtttaaat ttaatgtgtt ttgaactgat gacactatac ggcaacataa
    17101 aatattctta ttaaaactcg taaaatattc tctttttatt tgtaaattac aactattagg
    17161 ctcaaacaca acattagaaa tagctgtatc taaatctagc tctctttcat acaatgaaag
    17221 agcacaagac tgaatatcta tatgctcaat gcaaagttcc ccaagtaatt catagtggct
    17281 ttgatctctt ggggtagcaa acattgttaa ctttatcaat tcttcggctt tctgaatttc
    17341 tccatctaaa agatattccc gaattttttt aagatgcaat agtgagtctg gattatttct
    17401 atctgacttt cctctatacc aaatagtctc atcgtttagt tgaatacatt cttttgtagc
    17461 tgaaccataa atcataccac ctaaatgacc gttcccaatc ggcaaggctt cattccaatt
    17521 tgcggcagct ttcttatacc ataatttcac tctttacctc tcttccaaaa tggaggtgat
    17581 atcccaaata ccacctccac actttctact acttcaaaat tcctaaaaca actaggataa
    17641 ctccaatagc cataacacta aagattagta caaccggatt ctttcctttt ctaattaatt
    17701 tacacattaa taaagtcaaa gccataggga taaatcctgg aattaatttt gtaatcattt
    17761 cttgaacaga aataacaagt tccccctgct taaattctaa tccaaaatta ataccaacca
    17821 ttgatggaat caaaccaccc actagtacta gccctaatgc tgtcgccata ctcgtaacgc
    17881 gattcaatgt tccttttgta ttattttctt ggataagact agaaccctta gtatacccat
    17941 atttcaaacc gaaatattta acaggaatat taataatatt aaacaatatt aaggcgataa
    18001 agatacctaa agcaccgccg tctttagaat aagacgcacc aatactaaaa cagataggaa
    18061 ctagtgtcag ccagaacaaa ctatctccta gaccagccag tggccccatc aagccaactt
    18121 taataccagt aattgaagct gcaccttcat ttccttcttg ttcttccata gcggaagtaa
    18181 ctccaagaat aaatggtgcc gctgtttgat gagtattgaa aaactcaagg tgacgtttca
    18241 ttgcaggaga agctgaatct ttgtctgggt atagtttttt caatactgga agaattgtat
    18301 atagaaaacc taggttctgc attctctcat agttccatga agaaccatac ataaaacttc
    18361 gtttgaaaac ttttttaagt tcaactttag ttactttatt attcatcata gatcaagatc
    18421 ctcctcaaca tcactacttg aagttgtttc ttgtggttta cttccaatca tatcgtagaa
    18481 gtatgctacc gcaataccaa ctagtgagat cccaatggtt gacattcctc catacacaga
    18541 acaaatgaat ccaatcaaca agaataccca catatttttc ttgagcatca tatttaaaag
    18601 cataccaaaa ccaattgaag gtagtaaagc acctgcagag ttaagtccct gcataacgat
    18661 tggtggaacc ttagcaaaca agtctgccac atattcccct ccaagcataa tagctaggaa
    18721 aattggtaca attttaaata aagtgattaa aaccaaactt gaataatgat aaaaaccgat
    18781 ctttgaataa tctccgtttt tagcatcatt atcagctttt ttcacaaaat aaacatctaa
    18841 agtttttgca agaacatcca actgttgagt agcaactgca attggaactg ctatagcgat
    18901 accagcagtt tctccttgac cagacaaaat accaaatgca gtaccaataa tcgcacctga
    18961 aatagtatct ggtggagtgt aacctccaat acctgttaca ccgagccaag ttaattcaag
    19021 agctgaacca ataagaacac cttgggtgaa atctcctaag actaagccaa ccattgcact
    19081 tgtaactaac ggacggtgaa tcataaataa cggtccatta tagtcaatag tggcaataat
    19141 cccaactaat gtcaccagta atgcttgtgt gaatagcata tactgtacct ccttaaatta
    19201 tttttataaa aatttttcca acattgtaac tttatcatta ggaaccattt gaatttcaac
    19261 tctcgttccc ttatcaacta ttttcttaaa ataatatttg tcttcttcac ctagagctac
    19321 acctcccgtt accttttcat tttcctccgt tttactcatc tgtcctacat tgaggtactc
    19381 caattttaaa tttccttcaa tagaatcata cacatctttt ggatttgtat atatcagcat
    19441 tgttctcttt tttattggca cagagtttaa aacttccaca aaccgtttta cactaaagaa
    19501 aacaattttt aaacctaccg gtgcagaaat ctttaaaata gattgtcgtg ttttatcttc
    19561 tgagatgcga tcattaacaa tgataacttg ctcaatatca tactttttta gccacgtagt
    19621 gacaacttga ccatgtacca gacggtcatc aatacgaaca aactctatag acataaatcc
    19681 tcttcctcct ctacgttaag ttgttgttta acattaaaca aactattttg agcattttga
    19741 acaatttctt ctaaattgat ttttgaatca taacttgata ttaattccac taggagaggg
    19801 atattaaacc ctgttacaat atcaactgaa tccaaattta aaaaccgtga caaagccaca
    19861 ttattaggac ttcctccaat caagtcagtc aaaacgataa cctcttgatt tgagtctaac
    19921 agttcattta cttgattttt aaaataatgt tcaaactcta caatattctc tccaggcatc
    19981 aaacctaatg tcctaactct atcatttgtt gtctcaccaa caaacatttc tgcggtcatg
    20041 agagctccgc tagcaaaatt accatgactg gcaactaaaa ttcctctatt aaacattttc
    20101 tctttcaaca actactcctt tcttaaggat aatattagca taaagtgaag tttctcctgt
    20161 agcaacaata gcataagctt tcttactacg ttcataaaag tcttctcttc taagataagt
    20221 aatcgtttta agatctgtac catgaccttc aatcatctgt ctataggtac cccatatctt
    20281 aggaatatca tcacccgaaa caacgttcat aaactgaatt gaactatcga cgtaactatc
    20341 taatggcatt aaatacagaa tggaatctaa taattctgga atatttacgc catcacaacg
    20401 aattagctta tttgcacatg aggcagaagg ataattcgcg tcagctaata ctatttcatc
    20461 tccatgtccc atttccatta aagtcttcaa taaatctgga gaaatatttt tcggtatatg
    20521 ttttaacatt aatatcctcc ctattttctc tgaccgtatg ttttaaattt ttctgccatt
    20581 aaatccatct cctcatcagg aagaactact ggctctccaa aattcttagc taaacaataa
    20641 atttttgcac aatattcaac ttcttcaaca atattaaatg cattcaataa attttgtgca
    20701 cctgctaaaa ttccatgatt cgctagtaaa actgctctac gaccttccat tgctttagct
    20761 gcattcacag ccaattcttt cgtaccatat gttgcatact cagctacccg aacatctttc
    20821 cctgccactg caatcatata atgactagcc ggaagtggtt ctctgagaca agctaatact
    20881 gttgcataag ttgtatgagc atggataatt gcatcgatat catcacgagt ttgatattga
    20941 atcaaatgca tataccattc gctagatggc aagcgttctc cctctacaac atttccatta
    21001 atatccatca ctacaatatc ggattctttg atttcaaaga aatcaatacc cgacggggta
    21061 attgccatca attgtttttc acgatcgaaa acactgagat tcccacctgt tcctttcgtc
    21121 aaatctgttt ctactagctt cttgccatac ttaattaatt cttgttttac atctgacatt
    21181 cttgtcctcc tcatatcaaa acactaaata tttttcaatt caccaattat ccccccttct
    21241 tgtacaaaac ttaagaaggg ggagggaggc aaatttacaa actctcaatc tttttaattc
    21301 ttttgggagt aataactctc taattgtaga aactcctcaa taacatggtg agcttcttcc
    21361 atccctttta attgtcccat agctatgagc tgcacaacaa tattccccac agctgtaccc
    21421 tcagtcgaac ctgtaagaac ctctttacca gttctatcag caatcatttg gttaaagtaa
    21481 ctggctctag cacctcctcc aatcacatat atcctcttat aaaccttatg agttagttct
    21541 tctagtaact ctatcgcttt cctatacgtt tcagctaggc tttcataaac aatcttaaat
    21601 agttgtccat ctgtccactc tctagtttca tgatgataag ctagatattc tgtcaaagtc
    21661 ttgtgcatat cagattctgt tgcaaattca gttgattcag tatcaatcag aggaagattt
    21721 tctttttctt tctccaccat tgtcctaata tcatcaaaag aataggcttt ccctcttcgt
    21781 tcaaatgaac gtcttagttc ctctatgatc cacaaccctg tacaattctt cagaaatgta
    21841 atctctccat ctttaccgac ttcatttgta aatccataac tgaaggattc ggtagtaaga
    21901 atcggtgaag taagttccac tccaaccaaa gaccaagtac ctgatgaaat aaataaacta
    21961 ccttctgtct taggtactga gacaatcgca cttgctgtat catgactaca aacattcaca
    22021 acaggaatat cgcctaaacc atactccttt tttatccttc caagaacatt tccctctgaa
    22081 acaatttcag gaagtaaaga tgaatccaat tcaaatagtt ttaagatatt ctgattccaa
    22141 ttttgactcc taggatcaaa taattgagtt gttgaagcaa tgcttttttc tgtagcaaac
    22201 ttacctgtca agagataatt aaacaaatct ggcattaaaa gaatcttatt ggtcttatag
    22261 aaagagtcag gagattcttg acgtgcctta aagagttgaa acaaggtatt tatctccata
    22321 atctgatttc ctgtctctga atacagtttt tctaattcag tcatttcaga tatttccttt
    22381 aacactccct ttgttctttc atcacgataa tgaacaggtt gtgataacag cttaccttca
    22441 ttatcaatca gtccaaaatc aactccccat gtgtcaatac cgatagataa aatcttgtaa
    22501 ctagtattag ccaagcggat actttcaaga attttagcta gtagaaagtc aatatcccaa
    22561 gataaatgcc cttttactct aataggtaga ttagaaaagc gatttatttc ttccattact
    22621 agtttatttt cagaaaggta accaacgatt gctcttccag aagtcgcacc taaatctatt
    22681 gctaaaaccg cttccatttt tcttctctct tcgtccttga ttaactttat tataatcccc
    22741 acattttatt ttttcaatat gtgaacgctt acttgaataa tgttcaaatg ttaacccaat
    22801 attcataaaa ataattcaaa ttcctccaat ttgtgctata atatagttga aatgataaat
    22861 taggaggagc ttatgataaa agatgaacgt gtacttgaat tgattgaaat tattaaaaag
    22921 aaaaaaagaa ttgccgtaaa agagctggca gaaatcactt tctccagcac aagtacctta
    22981 cgtcgtgatt taattttctt agaaaatcaa ggtcttatca aaagaaagca cggatacgtg
    23041 accctgtcct ctatgaacac aattgaactt tctcatcaaa tacgggaagg agaaagtact
    23101 aggcaaaaaa gactaatcgc tagtctcgct aaagacttta ttcggtctgg tatgtgtgtt
    23161 tatctggatt ccagtactac tgtctacgaa ctttgtccct atctttctga acttgataat
    23221 ttgattattt ttacaaatgg tttacatact gcacaaaccc tatctgaaac tgttaaagat
    23281 agctccaaaa tctttatcac atctggcgag gtcaaacatc aatcctgttc cgtggtcaac
    23341 tatgataagg aaaattcttt attagatcat tttaatatcg atttagcatt ttgttcagca
    23401 agaggtattg atgaccaata tgtttatgaa gcttctctca gccaagctat ttcaaaaaag
    23461 aatattattg acaaagccca tgaaaccatc ttactgattg atagttctaa attttacaag
    23521 actggatttt ttaaaattaa tcccctctcc aaatacacaa cctttatttc agacaccgtg
    23581 ccagatcaaa aattattaga cgcagtcgaa ttatttgatg gagaatgggt ttctgatatt
    23641 caatgaaaat caaataggaa gctagtctca gcttgctcaa agtacagatt tgaaaactga
    23701 taaagtcagc tcgaaacact gttttgaggt tgtagataga actgatgaag tcagtcacat
    23761 atatacggaa agacgaagct gacatgatgt gaagagattt tcgaagagta ttcgttctgt
    23821 ctgctgactg atttacaaat attcaccaca tgacattatt ttctcagcaa ccctaaaaac
    23881 agaggctgga caaaaactca atttcaggag aaaatggagt aaaccttccc acaataaaac
    23941 gcatagtatc aagtttttgc acacctgata ttatgcgttt ttctgatttt aaagactttg
    24001 tcgcctaaac cctatatttc aagaattaag tgaccttgat gatctaaatg tattgtggta
    24061 tactattttt gtcatcggtt accgattacg ttcctatggg ttcgtgagag gaggtgaaac
    24121 taatgagcct tatcatagag ctcgctctta ctattatagc ggatgttata gctggaatta
    24181 tcttgtattt cgtctgcaga tggctagata gtaagaagta gaccgagtga ctagcctata
    24241 aacacccgtt aaatcgctaa gatacgtcaa aaaagccctt aactacggcc atagttaggg
    24301 gctttggtgt tctaatgaac cttatcaatt aactacattt tagcatataa tctacaatag
    24361 ttcaagcatt aagtgaggtt gatgatctaa atttagtgtg atataatact tttgtcatcg
    24421 gttaccgatt acgttcctat gggttcgtga gaggaggtga aactaatgag ccttatacca
    24481 gagcttgctc ttactattat agcagatgtt atagctggaa ttatcttgta tttcgtctgc
    24541 aaatggctag atggtaagaa gtagaccgac tgactagcct ataaacaccc attaaatcgc
    24601 taagatacgt caaaaaagcc cttaactatg gcactagtta ggggctttgg tgttctaatg
    24661 aaccttatac actaactaca ttctagcata taagtccaga tatttcaaga gttttattta
    24721 ttttttcagg ttcccttagt tctgaaaggt ctataatgaa gttagccatc tagtatcaaa
    24781 aaaccgacta gctcttatga actagtcgat ttctcatcaa tgcgccaaca tttcttgggc
    24841 gatttcttgg ccagataggt tatctgggta gtaggttggc caattatcca tttcttcaaa
    24901 tagagcttct tggcttgtac ctccaaaaaa gatatggaaa tgttctgcct taactggggc
    24961 aacattgtgg tcactaaact gaacatattt gaattgtcca gcgtcagcat ctgtggcttc
    25021 aaagaggaaa cgcacgccac gattgccttt cttgtaagtc aaaattttct taccgacata
    25081 cttgtaagtg tatttcttgc tttgtccacc ttgaacaaat tccatagtat tatcagtaat
    25141 gttaatctta gtcacatctg tctgatagcc ttttgtatag taagccttgt actcagcctg
    25201 ggtcatctta ccagtcaact tagccttgta gtcaaagact tggtcaaacg tgccgtcttc
    25261 aaggaaagga taaactgatt gccagttacc tgcatagtca ctcaaggtgc ggtccttgac
    25321 agctgcatcc tcgaagtaac cattttggac tgtcttggta tcctctgcct tttcaggttc
    25381 gattgctggg ccttcttggt ctgttgtttg tttcaaagcc ttgaggtttt tctccatcac
    25441 ggaaatgtag ttttctccag ccttggtgtc ctcttctgtc agactttcta aaggattgag
    25501 gacatcagtt ttgacacctg cttcttttga aagtgtgtta gcaagggctt gtgaggcatt
    25561 ttcttcaaaa tagatatagg cgattttatt tttcttgaca tactctgtca attctgccaa
    25621 gcgagcagct gatggctctg catctggaga aaggcctgag attgcgactt gtttgagtcc
    25681 atagtccaag gcaagatagt taaaggctgc gtgttgagtc acaaagctct tttgttttgc
    25741 ttgagacaag ccttctgtgt aagccttatc caaggcttgc aatttttcga tataggcagc
    25801 tgcattcttc tcaaaggtct cttttttatc aggataatct gctgacaagc tgtcgcggat
    25861 gtgctctact agtttaatgg cacgaactgg tgataaccaa acatgggggt caaactcatg
    25921 gtgatgacct tcttctccat ggtcatggtc tccctcttct tcctcgccac ctggcaagag
    25981 caacatatcg cctgtcgcct tgatggtttt cacttttttc ttatccaagg tatctagcaa
    26041 tttaggtacc catgtttcca tgttttcatt ttcataaacg aaggtatctg catcttggat
    26101 tttggcaact gccttggcag atggttcgta ttcatgaggt tctgtcccag caccgattag
    26161 gagttctaca ttagccgtat ctcctgcgac ttgcttggta aattcataga cagggtaaaa
    26221 ggttgtcacg atattgagtt taccatctgc ctgtttttga ttggaacaag ccactaaaaa
    26281 caaggcacat agactggcta gtaataagct aatttttttc acgttcgtct cctatttgat
    26341 aaaacgtctt actaaactga ttagtataaa gacagttaca aaaataatgg taatacttgc
    26401 acttgcaggt gtttctgcat agtaggaaat gtaaagtcct gctaccattc ccaaaaagcc
    26461 aatcgcactg gcaagcagca taaccgattt aaagtttttc cccagacgca gggcaatact
    26521 agctggcaag accataatgg tcgataccag aagagctcct gctgcaggaa tcataagggc
    26581 aatagccacc cctgtcacca tgttaaaaag aatggacatg gtacgaactg gcaagccatc
    26641 cacaaaggcc gtatcttcgt caaaagttaa gatatacata ggacgaagaa agagaaaggt
    26701 caaaatcaaa acaaccaccg caatgacaaa gagggaaatg acctgttctt cactgatagt
    26761 cacgatcgaa ccaaagagat attggtccaa actcattgaa ctcgagcttt tacccttgct
    26821 catgacaatc agagaaacag ccagacctgt tgacatgagg atagctgtcc cgatttccat
    26881 aaagctcttg taaaccgtac ggagatactc cagaaagacc gccgcaatca agacaatggc
    26941 aatagtagaa acagttggag aaatccccaa aaccagacca aaggctacac ctgaaagtga
    27001 gacgtggcta agggtatcac tcatcaaact ctgacgacgc aagatgagga aggttcccaa
    27061 taccggtgag aaaagactca tagcaataac cgccaaaaag gcgcgctgta taaagtcgta
    27121 agataataaa ctaagcatgg cccacctcct ggccattctc atgaacattg aaacaacgcc
    27181 atggcgagtc ttggttacgg actagatgaa tattgcgatc cgcataatcc ttaacttctt
    27241 cagggtcatg ggtaatcatc aaaacagcct tgccatgatg atgggcgctg tggtgcatga
    27301 gttcgtaaaa ttcattttta cttcctgcat ccatccccgt tgtcggctcg tctaggataa
    27361 acacatcagg gtcagaagca aacatacgcg caattaccgc tcgctgcttt tgtcccccag
    27421 atagagaccc caagcgtttg tctcgatgtt cccacatgcc aactgagtcc agactagcct
    27481 tgatatgctc ctcatcatga gcattcaaac gacggaacca gccttttctc ggatagcgac
    27541 ccgacttgac aaattcatag accgtacttg gaaaaccagc attaaaactg gcaatttgtt
    27601 gaggaagata ggctattctc aatttcttac cttgcgtatt tgtctttgaa atagccacct
    27661 ttccaatgcg tggttgcaga attccaagac tagccttgat gagcgtcgtc ttagccgctc
    27721 cattttcccc agtcaaggta acaaattccc cactatcaac acaataattg atatgttcaa
    27781 gaacaggctc cttatcataa tagaaggaca aatcctctac cgtaatatat ctcattattt
    27841 gatttctcct actaaagcag tcaaaaaccg ctgaatcact ttttgttcat ttggagtaaa
    27901 ctgagtcgcc acttgttcat aggttaaaag tgtatgctca tggtgatggt ggtgctcctc
    27961 agcgattgga cgagccaagt cagtcaactg ataaaaaatc acacgcgcat ctttagaatc
    28021 tttagatgtt tccaacatcc cttccttgac caaagactta atggccttgg taactgccgc
    28081 ctgactgaca ttgagacgac gagccaattc tgaatttgtt aaagattcct ctgacaagag
    28141 cataaggata tgctcctgag tattggtcag ggccacctcg ctagtgcaat gacctattag
    28201 gatttcatgc tgattttccg cctgcaaaat cacctcattc aaaaaagcat tgatatcctt
    28261 tgctagctgt ctcatatctg actcctttcc ttttagactt ctctttttta agagaaaaat
    28321 actattcttt gacattttgt ttaccagtta attatatcac aagcaaaaaa agagtcaaga
    28381 aaaaacgtga aaactagttt cattcttgaa ctcttctata ttatattatc tattgaaatt
    28441 ctttgacatc tccatcataa gtcgcccaat ctttgctgaa aaagcgctca ttcagatggt
    28501 aagtcggagc tggtgtggga ttggatagga aaggatcaac tgccttgtca aaagccaacc
    28561 aacccaacca accaaggtga atggtgtcct tcataaagaa aggctccccg ccgtccttag
    28621 aaaaatctgc tatattggta aaaccttgac tttctaactg gtagcgaatc ttctgcaccg
    28681 tttgttggta catatcctct cgtagaccag catagtccat ccatttttta ttaacaggtg
    28741 gaatgataaa aatcgggttt accttagatt tagaaaactg tgttaaaacc aactgcaagt
    28801 cattatactc tggcgacttg agataggtaa agcttttctg agaatccttt aatttcttca
    28861 aatccttctt gatctgctca ttatagaaat aattttccat tcccatctca ttattggaag
    28921 tatttttttc agcatctgct ttgacaacat cttctattgc ttgataagaa aactggtctg
    28981 gcaagatttt taaatactta gctacatgct tatcgtagtt aacatagcct ctaaccgaaa
    29041 actgaccaaa aaaggaggcc tggcgttcat taaaacgagc caataattca atcatttcat
    29101 tgtctgctgt cgacaattct tctttacttg ccaacttctg aaccaggtcc ttcatagcta
    29161 cgtttgggaa ctgttgcagt aagcgagtcg ctgcatattg actagcctga tccccagatt
    29221 gatgtttcag aaaactagtc aactggtctc cattaaaata ctgctggaag gctgctggat
    29281 catagccatt tttactgaac cactgaggtg agataacata cacaacttgt ttattctcca
    29341 gctgttgtaa catctgttgc attccaaaat attggttaag cgatgcagct cccccctgtc
    29401 ctaaaagata aggacggtag gaacgattgt atttctcagc taataccgca ggatgagcac
    29461 cgtcaaaacg aagccattca ctagagccaa agaagggaac aaaacgcaca tttggatcag
    29521 atagtgctct gactttttga cttcgctcct taaaactatc gatagtagta gccactgctg
    29581 aacgcttttc agctcctaga ttatgatgca tctcagtagg ataaaagaaa atgagcagaa
    29641 aaaccaacaa accagcgatc aagaccggtc cgaagatcat ccataagcgt ttaagcattt
    29701 tgtagctcca caataccagc tataatttta ttagctgtat tccagtcgtc acgaccaaac
    29761 tctgttacag ggacacgaat gtcaaaacgg ttctcaatct ccacaatcaa ctcaaccgtt
    29821 cccatactat ccaagacacc tgcatcaaaa agatcttcat ccatcatgtc agaaacatct
    29881 tccataaaca actcatcaat aatttcgata acttctgatt tgatatccat attttatttc
    29941 cttttatttt ttaaaccata gattattcaa gaatccagaa aagattaaga atgacaacat
    30001 gacaacatgg aaagtgacaa ccatgccaag caactgaatc cagcgattct caggtagggc
    30061 agccttccct gcttttttcc gttccttatt gagcgttttt ttcttgcgaa cccaggcatc
    30121 attgatgacc aaacctagtc catgaaagag tccataggcg atatagtacc aggtcacgcc
    30181 atgccaaaat cccataatca gcatatttac aatgtaggcc atgcttgagg ttacattacg
    30241 atttttaaag actttctttc tggttaacac catcaccatt cgcataaaga caaagtcacg
    30301 gaaccagaag gacagactca tatgccagcg attccaaaac tcctttaaat cccttgataa
    30361 aaagggcttg ttaaagttga tagggctacg gattcccatc aagtttgaga tggccaaagc
    30421 aaacatagaa taacctgcaa agtcaaagaa gagctccaga ccaaaagtat acataactgc
    30481 caaggcatag agattaaaga agccacctga ctgcaaggct aaattcttca gaggaggtag
    30541 taaggtctct cctaaaacat gagctaggat aaacttatac aaaaagcccc gcatgatata
    30601 gcggacagat tcatccagca tatccatcaa ctcatctcgc tcaggaatgg tctgataatt
    30661 ttcattaaat cgcttaaagc gatcgattgg accactcgag aaagttggca tgaagagaag
    30721 gaaacggagg aattcccaga gggtaaaatc cttaatcact ccatctctca gctcgatgat
    30781 aatcccaacc gaacgaaagg tcaggtaaga aattcccaag aacccaagca aagactgcgt
    30841 tccattgata gctggttgca ccttgacaaa gataatcgga agtagggaca gaaaactaac
    30901 taagtagaag acccacttgc catccttgct ttttcgataa tgcttgtaga aaagcaggag
    30961 caatatttcc cagcaaaggt aaatacccaa ggcagctagt tgattggtct ttccacccac
    31021 caacatggtg acaataaaga agagacttac caacacttca taccaggcaa agcgtttctt
    31081 gaaaaagagg cctataaaga tgggcaaggt tgcagcaatc acataaacaa aatactgagg
    31141 attaccatat ggctctaaat gaggaagctg ttgaaaaaac tccatcatct cttattcacc
    31201 tcgttaatca atcctttgat gtcaatcttt ccatttggag ttagtggcaa actgtctcgg
    31261 taaaggaatt tagatggcat catgtaggac atcatgatgt ctgtcaggtc ttccttgatg
    31321 gccttggtaa tatcgatatc tcgctcaaac tgctcacgaa caccgtcttt taagatgaca
    31381 taagccaata gattttgtac cttgtggtcc ttgttatagc gcggtactgc gacagcagat
    31441 tcgataaagc gagacttgtt gaggttttga gagacatctt ctaactcaat gcggtaaccg
    31501 ttgaacttaa tctggaagtc catgcgtccg ccgtagagaa gcaagccctc atctgtcatg
    31561 gttcccacat cgcctgtgtg ataggctggc agattttcaa actcaaagaa ggcttctgct
    31621 gttttttcag gattgttcat ataacctttt gaaacagctg gcccagaaac aatgatttct
    31681 ccctgctcac catttggcag tttatttcct tcctcgtcaa tgataaaggt tggagaatca
    31741 gccttggtat agccgattgg taggcgtttg agagtcgcta acatctcgtc tgtcacggca
    31801 actgctgaca gagctactgt cgcttctgtt gggccgtaag cattgatgat atgggcattt
    31861 gggaaacgct cgcgcagttt ttgagctgtt ttgaccgtca attcttcacc atcaaagtag
    31921 aaatgagtga ttccaggcat tttctcactg ttgaagtatt cagacaacat ggccatatct
    31981 gcaaaggatg gtgttgatgt ccagatagcg attggcaatg aaaagatagc cgcaaagagt
    32041 tgcttaaagt cctgagtgat gactgaagga agagtgaaaa gcgtaccacc aagtgccaag
    32101 gtcggtgccc aatacatgac agacaagtca aaagaataag gtggctgtgc cagcatttgc
    32161 ggacgactcg gtgtcgcaaa ttccttatcc gtaatcatcc agtttgtaaa gctgaggaga
    32221 ttatcatgtg aaatctgcac tcccttaggc ttaccagtcg taccagaagt aaagataatg
    32281 tagtaattat catctccctt gactggatgc gtgatttcat agttattccc ttgggcaaag
    32341 gcttcttgaa cctgagctag atttatcatt ggtgtagaaa cctgctccaa gggaaaggct
    32401 gaaatggcaa taatcaagct tggctctgct acttctaaaa tagctgaaac tcgctccaag
    32461 gccgaatggc tatcaattgg aatgtaggca tgacctgact tagtcagcgc tacaaaggtt
    32521 gccaacattt catattcttg gccaccaaaa acaaccacag gagacttctc aggcaagcct
    32581 agttggtcaa tgactgcagc caaactatcc gaatcagcct ttaaatcgcc ataagtgtgt
    32641 tcctgcccca aaacattata gacaggatag ctaggctgtg tctgagcaaa atgctcaatg
    32701 gtttcaatca tatctgctat tggtttattt gacacaatag ggattctcct tcaagttaaa
    32761 attcattata gataaagctt ccttgaccct gaccaagata gctaaagaag taaagcagcc
    32821 ctagaaagat aagaaaatac aaggctgtcc gaccaagaaa gaggtacaat tcttttctct
    32881 gtttcatcaa gaaaaaccat tcatttctgt aatttttcgc taaaataaga gtgattctta
    32941 ctagcttatt tttctaccat tgtaccactt tatatagtat cttttcaatt gtttaccgta
    33001 tgtttccaat agatttcagc ttattttaag gattatacag tttttctatg tatattttca
    33061 aatagagtga tcctgcttca aaactccatt tcaggagaca atgaagtaaa tcttcccata
    33121 ataaaacaca caatatcaag ttttttcaac acctgatact atgcgctttt ctgattttta
    33181 aagacttttt aaccactctc tcatttaaaa taatctcgtc tgatataaat taaaatagct
    33241 tctatcatca gacaaatggc tgatagccaa aaactgatgc taataccaaa actctcagta
    33301 atatagctca ttagcaaaac aaatactgaa aatgctaatg tagaaatcac ttcaagaacg
    33361 gaatagacat taactaaatg attttcctct actgtttcct gaagaaatac actttcagga
    33421 acttctttta gttgcgataa cataccaact aaagctgaaa ataataaaaa catctgtgcg
    33481 tttggaaaat atagaatagt cagtgtcact attgccatag ctacaagagg aaaaagaata
    33541 ctttcccatt tagcagcaag gaacttttca gatagcctaa aagcaattaa gccactaatt
    33601 ataataccaa tagagtatgc tgtattagaa tatccccagt aactttccgt tttatttaat
    33661 aactccgtta caaaaacaag tataatggaa gaaacccaaa tcgtatttga aaaaatttcc
    33721 aataaatttg ctgatacaaa aagtcttaat ctaggatttc tagcaactaa cttccaacct
    33781 ttgagcaaaa tttcaagatt agtttctgac tctaacacct ccacttcagc gttaggaaga
    33841 aataacatca gaaagctaga aatgatatac aagactaaaa tgataaacgt ggtaggtaac
    33901 agaccaattg ttgcaaacaa gagtccacct aatccccaac ctatcaattg aacagcttca
    33961 ccagtcattg ataaggctga attagcctta cccaaatcgg tcgcatagcg tggcacaata
    34021 gcataggaaa caggtgctgc aaaaccatct agtatggaaa ttgcaacaac aaatagatag
    34081 gtcaccaaag gcgctacgga ttgcattacg gtaaacattc ctaccagtat cgccaataat
    34141 atagtctttc caaattgaga taaagataaa accctattta gcgctaacct tttagtaacc
    34201 aacggaacta aaagactcgc aacaaaagag gatattccta ttaagatagg aactagtgat
    34261 gtggcaatta ctgattttga aataatgtat atgttagcaa tgattgttac tctaaagaaa
    34321 atatctgcta aatttgcaaa taattgagag actaacaact tgacaaatga attagacatg
    34381 tagcgctccc cctttagtta caaactttgg aattaaattt gatttttaat attttagcat
    34441 aaaatagcag aaaacaaaag tgaggttttt tacatgaaaa aacaaaaact attgccttta
    34501 ttattcctaa ttttaggagg aattatgatt atcgttgttg gctagtacta agaagttagt
    34561 tagttagtag aataattaca tcttcaatcg aaaaagagac tggaaaaagt cttaaaaatc
    34621 agaaaaacgc tcaaatcctt tggcgctcta agcttttcta atgatagatt tagatagtca
    34681 aaaaaatatc tgaaaagttc ccaggatcgt cgtctcagca tcaaaaatac gtgaaatttg
    34741 cataattaaa ataaatttca ggggagttaa tcgagtaggg gataatctct agcccctctc
    34801 acaccaccgt acgtgccgtt cggcatacag cggttcaact aacttttaac gcatgtcgtt
    34861 caaggtaata atccaaacac gaaaccagtc cacgtttttc caggactggt tttgatatag
    34921 cacgtttaag taccgacttc tgagctacta attgataatg gtcgccccag ccagatacct
    34981 tatctgctat ccatttagga acccctaact taagcaatcc ccataatcgt ctcgatttct
    35041 tcttccattg cttccagata atcactcgta ggcgagtacg caagcgctca tctatgctgg
    35101 cgactatact tttcatattt cccaatgaga aatagtttat ccatcctcga atagacaaat
    35161 tcagttgctc aatacgtctt gttaggtcta tactccattt cctctgtgtt agtttcttca
    35221 atttaagctt aaatctccga acactatctt gatgtggacg acttttccaa ccatctgatg
    35281 atttccagaa cccaaaacct agatatttca actctcttgg tctggtaatc ttagtcttgg
    35341 tcatgtttgc tttcaaacct agccgttttt caataaaacg actgactgaa tacatcatac
    35401 gcttagcggc tgcctcgctt acgaccgtaa tcacacaatc atctgcgtag cgcacaaatc
    35461 gaagtcccct cttttctaat tccttgtcca attcattaag catgatattg gataagagag
    35521 gagataaatt tcctccctgt ggtgtaccaa ctagtgtttt ataacgttga ccattaatga
    35581 taacacctga atgaagatac ttacgaatca aggattccgt atctccgtct tcgataatgt
    35641 tatgtactaa ggacatcaat ctatcttgag gaactgtatc gaaaaatttc tctaggtcta
    35701 tatccactat ccactcatag ccgtcattta agtattctaa gagcttcatg atggcttttt
    35761 cacatgacct atttggtctg aaaccataac tcgtatctga gaaatggggt tcacaaatgg
    35821 ggctcatgac ttggacaatg gcctgttgaa tcattctatc cataactgtt gagattccta
    35881 gttgacggat gcctccgtct ggtttaggta tctcaacttt aagaactggt tgaggcttat
    35941 attttctctg ttttatcagt tccttagtca agcgccagtt ttgtctgaga taattatcca
    36001 tctcttcgat agtcattcca tcaatcccag ctgagccttt attggatttt acttgattgt
    36061 aggcttccag catattttcg cgtgataata tcttatctag cagttttgac atgtgcgtat
    36121 tcctttcttg ttttggtgcc tgagggacat cttatattgg tgaaccttca tctagtcatt
    36181 acgtgtcaaa gtccagttct atcagaccac tcattttaca gacacaagtg aagtaggatt
    36241 acttcgatct gtcgttttct ctttctagcg acagacctca aacagtctct ccccagactg
    36301 tttgagtatt caccccttcg ctccacttcc actacagaag tttcctcact actatgggct
    36361 cggctgactt ctcatgattc gttgttacta cgctttcagc gctcatgaaa cctcacggga
    36421 taagtcgtac atctttcctc gtttatacaa gatatgagga cgttaaaagg ttacagtgaa
    36481 aataggaaat ctgacgcaga gtctttagac tctagaaaga tttatctttt tcacacaaac
    36541 cttagtccga attcaattac tctcgtgatt tacacatata ggttacgact acctttttgg
    36601 actttagagt ttgaagcctc cttatccgct atatacgcct tactatcacg ttcctgttcg
    36661 tagagccacg atttcgctat cccttcctct gacgattctt atttcctgcg tcagacttaa
    36721 aacggtctat ccccagaccg ttttaatccg ctacctcacg atagtcaggc ttgggaatcg
    36781 ctattgggtt caccggtagc aggtgcccac cgaggactta cac
//
LOCUS       DAVLVF010000014        35513 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVLVF010000014 DAVLVF010000000
VERSION     DAVLVF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.014"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(111..1745)
                     /locus_tag="VYN66_000635"
     CDS             complement(111..1745)
                     /locus_tag="VYN66_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494697.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEMPIYFSSMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1726..2475)
                     /locus_tag="VYN66_000636"
     CDS             complement(1726..2475)
                     /locus_tag="VYN66_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494698.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGKVED
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            2654..4108
                     /locus_tag="VYN66_000637"
     CDS             2654..4108
                     /locus_tag="VYN66_000637"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV1494699.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQQDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4198..6816)
                     /gene="alaS"
                     /locus_tag="VYN66_000638"
     CDS             complement(4198..6816)
                     /gene="alaS"
                     /locus_tag="VYN66_000638"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV1494700.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPCITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6838..7323)
                     /locus_tag="VYN66_000639"
     CDS             complement(6838..7323)
                     /locus_tag="VYN66_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV1494701.1"
                     /translation="MKTFLVKQKFRLGGERFAIKNDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7598..7837)
                     /locus_tag="VYN66_000640"
     CDS             complement(7598..7837)
                     /locus_tag="VYN66_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494702.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8098..9168)
                     /locus_tag="VYN66_000641"
     CDS             complement(8098..9168)
                     /locus_tag="VYN66_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1494703.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIKGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9165..9938)
                     /locus_tag="VYN66_000642"
     CDS             complement(9165..9938)
                     /locus_tag="VYN66_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1494704.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9935..10741)
                     /locus_tag="VYN66_000643"
     CDS             complement(9935..10741)
                     /locus_tag="VYN66_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1494705.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10722..11879)
                     /locus_tag="VYN66_000644"
     CDS             complement(10722..11879)
                     /locus_tag="VYN66_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494706.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12039..12944)
                     /gene="murB"
                     /locus_tag="VYN66_000645"
     CDS             complement(12039..12944)
                     /gene="murB"
                     /locus_tag="VYN66_000645"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV1494707.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13056..13808)
                     /locus_tag="VYN66_000646"
     CDS             complement(13056..13808)
                     /locus_tag="VYN66_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV1494708.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13818..14549)
                     /gene="budA"
                     /locus_tag="VYN66_000647"
     CDS             complement(13818..14549)
                     /gene="budA"
                     /locus_tag="VYN66_000647"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV1494709.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14691..15962
                     /locus_tag="VYN66_000648"
     CDS             14691..15962
                     /locus_tag="VYN66_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV1494710.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     SQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLKELERHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15982..16797)
                     /locus_tag="VYN66_000649"
     CDS             complement(15982..16797)
                     /locus_tag="VYN66_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1494711.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
     gene            complement(16954..17607)
                     /gene="phoU"
                     /locus_tag="VYN66_000650"
     CDS             complement(16954..17607)
                     /gene="phoU"
                     /locus_tag="VYN66_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1494712.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17619..18377)
                     /gene="pstB"
                     /locus_tag="VYN66_000651"
     CDS             complement(17619..18377)
                     /gene="pstB"
                     /locus_tag="VYN66_000651"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1494713.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18390..19193)
                     /gene="pstB"
                     /locus_tag="VYN66_000652"
     CDS             complement(18390..19193)
                     /gene="pstB"
                     /locus_tag="VYN66_000652"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1494714.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19204..20088)
                     /gene="pstA"
                     /locus_tag="VYN66_000653"
     CDS             complement(19204..20088)
                     /gene="pstA"
                     /locus_tag="VYN66_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1494715.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20078..20995)
                     /gene="pstC"
                     /locus_tag="VYN66_000654"
     CDS             complement(20078..20995)
                     /gene="pstC"
                     /locus_tag="VYN66_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1494716.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(20995..21873)
                     /gene="pstS"
                     /locus_tag="VYN66_000655"
     CDS             complement(20995..21873)
                     /gene="pstS"
                     /locus_tag="VYN66_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV1494717.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22447..23751)
                     /locus_tag="VYN66_000656"
     CDS             complement(22447..23751)
                     /locus_tag="VYN66_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV1494718.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23752..24525)
                     /locus_tag="VYN66_000657"
     CDS             complement(23752..24525)
                     /locus_tag="VYN66_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV1494719.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24515..24793)
                     /locus_tag="VYN66_000658"
     CDS             complement(24515..24793)
                     /locus_tag="VYN66_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV1494720.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24793..25194)
                     /locus_tag="VYN66_000659"
     CDS             complement(24793..25194)
                     /locus_tag="VYN66_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV1494721.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25404..26021)
                     /locus_tag="VYN66_000660"
     CDS             complement(25404..26021)
                     /locus_tag="VYN66_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV1494722.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(26011..26784)
                     /locus_tag="VYN66_000661"
     CDS             complement(26011..26784)
                     /locus_tag="VYN66_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV1494723.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26795..27532)
                     /locus_tag="VYN66_000662"
     CDS             complement(26795..27532)
                     /locus_tag="VYN66_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV1494724.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(27537..28667)
                     /locus_tag="VYN66_000663"
     CDS             complement(27537..28667)
                     /locus_tag="VYN66_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV1494725.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28751..29140)
                     /locus_tag="VYN66_000664"
     CDS             complement(28751..29140)
                     /locus_tag="VYN66_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1494726.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(29156..29539)
                     /locus_tag="VYN66_000665"
     CDS             complement(29156..29539)
                     /locus_tag="VYN66_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV1494727.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(29540..30328)
                     /locus_tag="VYN66_000666"
     CDS             complement(29540..30328)
                     /locus_tag="VYN66_000666"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV1494728.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFSLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEK"
     gene            complement(30321..31256)
                     /locus_tag="VYN66_000667"
     CDS             complement(30321..31256)
                     /locus_tag="VYN66_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV1494729.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31386..31562)
                     /locus_tag="VYN66_000668"
     CDS             complement(31386..31562)
                     /locus_tag="VYN66_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV1494730.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31702..32409)
                     /locus_tag="VYN66_000669"
     CDS             complement(31702..32409)
                     /locus_tag="VYN66_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV1494731.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            32560..33588
                     /gene="queA"
                     /locus_tag="VYN66_000670"
     CDS             32560..33588
                     /gene="queA"
                     /locus_tag="VYN66_000670"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV1494732.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFY
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            complement(34249..34638)
                     /locus_tag="VYN66_000671"
     CDS             complement(34249..34638)
                     /locus_tag="VYN66_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1494733.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(34692..35384)
                     /locus_tag="VYN66_000672"
     CDS             complement(34692..35384)
                     /locus_tag="VYN66_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1494734.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVFVPFATKYEADNDLSAGQEQEITLGKNGKIVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEISIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSIL"
BASE COUNT        10552 a         8141 c         6742 g        10078 t
ORIGIN      
        1 acattcctcc ctatattttt gattaccaat cttaatcatt tacaactaca ttctaacaaa
       61 ctataaaagc gtttgtcgaa ttgaatttat caagcaagca accaactatc tcatcttttt
      121 tctatttctg ccaatatgcg tgacaggtaa taatgatagc caaaaatagc aagagcaagc
      181 aagacgataa gagctcctac tcccaagttg atggcaagga taggggagag agactgaacc
      241 aagaatatgc tcccaattac aagggccatc aggattgcac tataaataaa caataaaact
      301 atggcgacta tgccatttga acgattcacc aggtccgtaa tgctactcca attggttgac
      361 agatttttaa cgtccttaaa gtaatggtgg caagaaagga tgacactggc aatgatccag
      421 actacaagaa ggtaaatcat cgaaaggatg gacaagccta gatatagaga aagaccgagc
      481 aaagtcagaa ctggtaaaaa ggactgaaca gcaaagataa tccaaaattt cactttcaca
      541 taacgagcaa agtcaaaggg taaactcttc agaaaatcaa cattttccct ctccaaggac
      601 aaggcaattg aatgcaggct ggtgatattg ttattgacaa ctgctataaa cactcctaca
      661 aaaaacaagg gtaaccagta tggaggatga atgtctggaa ctatctgaga atctcggatt
      721 ttggaaatca gactgatcat catgagataa ggaaggaaag cacttgtaaa aagcactgta
      781 atcacaccag tcccctgtcc caagagggtg aggtggtagc gtaaaaccat acggaaaaag
      841 ccctttttag tagttgaaat cctctccttg ctgcgacgtt cttttttgac cttctcctca
      901 ctgttaagca ggatcacgtc ataaaaacga ggaaggacct tctttttggt cagataaagc
      961 aggaagagag ttagtcctat ccaagcgagc agacccacta aggcttctgt cgaaaaaggc
     1021 tccactgcta ttttgtaaaa gatatgaaga ggataaagga gaaatggaat gtctctaact
     1081 ttgtcaacaa tacttccaaa agtcgactga agaaagaaga taaatattaa aggtatgaga
     1141 actcctatcc caatcatcac attcgaaaaa atagactgat actttctgaa gaccctagtc
     1201 tgagccaaga aatgtactgc cactaccgtc actaaagtaa cagagacaaa taataaggtc
     1261 aaggacagta gcatcaaagg caaacccagc caaagagaag gagctagact aatatagagg
     1321 gctagaaaat aagctaggat tggtacaatt ccagttagag ctggcaagag gacagacaga
     1381 cctttggcca ttataatctc tgattcttta aaggcatagg gcctatagga ggccaggtcc
     1441 ttactctcat aaaagacatt gtaaaaggca gtgaaagaag ttgaaaaggc cagcaccagt
     1501 aaaatagcaa ccatcgagct aaaataaata ggcatttcct caaaaggaaa atgaatggct
     1561 atattactaa aacagatgag catcaagaga ctggaaaaaa tgtaagaact taggactcta
     1621 gcagaaacat ttactttttt cccaggattc ttagcctgct tctttcgtag attagccaga
     1681 ttagcttctt gagatgaata gaggatattg atatcaacta attttttaat gaccttgaga
     1741 cgcatccgca acctcctctt ttctaccagc aagactaagg tagatacttt ccaaaaactg
     1801 gtctgggtgg tctttcctca agtcctctac cttaccacaa taaatcaaat gccccttttt
     1861 caaaatggca atccgatcac agacttgctc tgccacctct aggacatgag ttgaaaacaa
     1921 gactgtcttc cctttttgtg catgttcctt catcatctgt ttcaaatcaa aggcagcctg
     1981 gggatccaaa ccagtcaagg gttcatccaa gacccaaata tcgggatcag acaagagtgc
     2041 tccgatgaca aagactttct gacgcattcc gtgagaaaga gtttcaataa cctgatagcg
     2101 attttcagca aaatcaaaaa cgttcaatag cctagctaga ctagcctcca agtcagatct
     2161 actcagatca taggatgagg cgatcaattc ccaaaattca ttggccgtta agcgtaaaaa
     2221 taagtcaggc gagtctgcta cgtagccaat ctttcgttta atagccaagc gattttccga
     2281 taactcctga ccgtctacca aaatacgacc actgctgggt gaaatgatac tgactaggga
     2341 ttttatggtg gtcgattttc cagccccatt atgaccaatc aagcccataa tctctccatt
     2401 ttcaatctgc aaattgagat tactcaaggc ctctttatca ccatacaact tactaacatt
     2461 ttgaaattca atcatgggac aactcctatc tttatctgta ttatacatcg ctatactagc
     2521 actttgatac aaaaaaatca atactttatt ttaagtagtt aaaatagttt ccttctatct
     2581 tacgcaaacg tttgcaccaa gagatacttt atgatagaat aaagaacaag attgacaagt
     2641 aagaggaaac atcatgcaaa atcaaacact catgcaatac tttgaatggt atctgcccca
     2701 cgacggtcaa cactggacgc gtctggctga aaatgctcca cacctagctc atctggggat
     2761 cagtcacgtc tggatgccac cagccttcaa ggcaaccaac gaaaaagatg tcggctatgg
     2821 ggtctatgac ttatttgact taggagagtt caaccaaaaa gggactgtcc gcaccaagta
     2881 tggtttcaaa gaagactatc ttcaagccat tcaagccctt aaagcacagg gaattcaacc
     2941 tatggccgat gtagttctca accacaaggc tgctgccgat cacagggaag cctttcaggt
     3001 catcgaagtt gatcctgtag accgtacagt tgaacttgga gaacccttca ccatcaatgg
     3061 ctggactagt tttaccttcg atggtcgcca agatacctat aatggcttcc actggcattg
     3121 gtaccacttc accggtacag actacgatgc caaacgcagt aaatctggga tttatctgat
     3181 ccaaggggac aacaagggct gggctaacga ggaattggtc gataacgaaa acggaaacta
     3241 cgactacctc atgtatgccg acctagactt taaacatcct gaagtcatcc aaaacatcta
     3301 tgactgggct gattggttca tggaaacgac tggtgtagct ggtttccgtt tggatgccgt
     3361 taagcatatt gactctttct ttatgcgcaa cttcatccgc gatatgaagg aaaaatacgg
     3421 tgacgatttc tatgtttttg gtgaattttg gaacccagac aaggaagcca atctggacta
     3481 tctcgaaaaa acggaagaac actttgacct tgtcgatgtt cgtctccacc agaatctctt
     3541 tgaagccagt caagctggcg caaactatga ccttcgtggc attttcacag atagcctggt
     3601 tgaactcaag cctgacaagg ctgtgacttt tgtcgacaac cacgataccc aacgaggaca
     3661 agcccttgag tctaccgttg aagaatggtt caagccagca gcctatgccc tcattttgtt
     3721 acgccaagac ggccttccat gtgtctttta cggagactac tatgggattt cagggcagta
     3781 tgctcaacaa gatttcaaag aaatccttga ccgcctccta gccatccgaa aagatttggc
     3841 ctatggagaa caaaatgact actttgacca tgctaactgt atcggttggg tacgttcagg
     3901 tgctgaaaat caatccccaa tcgcagtcct tatctcaaat gaccaagaaa acagcaagtc
     3961 aatgtttgtc ggtcaagaat ggactaatca aacctttgta gatttacttg gtaaccacca
     4021 aggtcaagtt acaattgatg aggaaggtta tggacaattc cctgtctcag ctagatccgt
     4081 aagtgtctgg gcagtcaata ccatctaata gctcataata accaagctag gtccaagcgg
     4141 atttggcttt tttgtattca caaaaagacc tacccaaatg gatagatctt tacttgatta
     4201 caatttacct gctactgcat ccaacaattc ttggatctta gcttggttgc ttcctcctgc
     4261 catggccatg tctggtttac caccaccacg tccatcgatg attggtgcta attctttgac
     4321 aaggtttcct gcatgaaggt cttttgtctt gcttgctaca aggacattga ctttgtcacc
     4381 gatagcagca actaggacaa gaagatcaga gtagtctttt tgtttccagt tatctgcaaa
     4441 ggtacgaagg gcaccggcat ctgatacaga cacttgacta gcaatgtagc ggtgaccgtt
     4501 gacttccttc acatccttga agatatcgcc tgcggctgca gctgcggctt tttctttcaa
     4561 ctcagcattt tctttttgaa gttgacgaag ttgttcttga agtccttcta ccttgtgagg
     4621 tacttccttg acttgaggtg ctttcaaggt tgctgcaaca gctttaagag cgtcctcttg
     4681 ttcacgatag gcttcaaagg cttccttacc agtcactgcc aagatacgac gagttcctga
     4741 accgattcct tcttctttga caattttgaa gagaccaatc tcagaagtgt tgccaacgtg
     4801 ggtaccacca caaagttcaa tagagtagtc accgatagtc acgacacgaa cttccttgcc
     4861 gtatttctca ccaaagaggg ccatcgctcc catttcttta gcagtgtcaa tatccgtttc
     4921 aactgtctta acttcaagag cttcccagat tttttcgttg acttgctgtt caatcgcacg
     4981 aagttcctca gcagttactg cttggaagtg ggtaaagtca aagcgaagga attcaacttc
     5041 gttaagagat cctgcctgtg ttgcgtggtt tccaaggata ttgtgaaggg cagcgtgaag
     5101 caaatgagtc gcagtgtggt ttttcatgac acggtgacgg cgattgctat caattgccaa
     5161 ggtatattct tggttcaagg caagcggtgc aaggacttca actgtatgaa gggcttgacc
     5221 atttggggct ttctgaacat tggtcacagt agccacaacc ttacctgact catccaagat
     5281 ttgtccgtag tcagctacct gtccacccat ttcagcataa aatgacgttt ccgcaaagat
     5341 aagagaggca gttccttctg aaacagcttc tacttctgca ttgtccgcca cgatagctac
     5401 caatttagaa gacaattggc tagcattgta gttgaagaca ctttctacag tgatgttttg
     5461 aagagtttca ttttgcatac ccattgagcc acccttgaca gctgaggcac gcgcgcgttc
     5521 ttgctgttct ttcatggctg cttcaaaacc ttcacggtct acagtcatac cagcttcttc
     5581 agcgatttct tcagtcaatt caactgggaa cccataagta tcatagagtt tgaagacatc
     5641 tgaaccagcg ataacagatt gacctttttc tttcaagtct gctacaatgc cttgggcaaa
     5701 gtgttgacct gagtgaaggg tacgggcaaa tgattcttct tcgctcttaa cgattttctc
     5761 aataaagtca cgtttctcaa gcacttctgg gtagtagctt tccatgattt ttccaacagt
     5821 tggaaccaat ttgtaaagga aaggctcgtt gatacccaat ttttgaccat gcatagaagc
     5881 acgacggaga agacgacgaa gaacataacc acgaccttca tttcctggaa gggcaccatc
     5941 accgatagca aatgaaagag aacgaatgtg gtctgcgata accttgaagc tcatgttgtc
     6001 gccatcttgg tcataaacct taccagacaa tttctcgact tcacggataa tcggcatgaa
     6061 gaggtccgtt tcaaagttgg tcttagcccc ttggataacg gccaccaaac gctccaaacc
     6121 agcgcccgta tcaatgttct tatgtggcaa ttccttgtat tcgctacgag gaacagcagg
     6181 gtctgcgtta aattgtgaca aaacgatgtt ccagatttca atataacggt cgttttcaat
     6241 atcttctgca agcaggcgaa gaccgatatt ttctgggtca aaggcttccc cacggtcaaa
     6301 gaagatttct gtatctggtc cagaaggtcc cgcaccgatt tcccagaagt tgtcctcaat
     6361 tggaatcaag tgacttggat ccactcccac ttcaatccag cggttgtaag aatctttatc
     6421 gtctggatag taggtcatgt aaagtttttc agcagggaaa tcaaaccatt cagggcttgt
     6481 caaaagctca taagcccaag tgatagcttc gtcacggaag taatccccga tagagaagtt
     6541 ccccagcatt tcaaacatgg tatggtgacg cgcggtcttc cctacgtttt cgatgtcgtt
     6601 ggtacggata gccttttggg cattggtaat acatggattt tcagggataa tggtcccgtc
     6661 aaagtatttc ttaagggttg ctaccccaga gttgatccac aaaagagttg ggtcatttac
     6721 aggaaccaaa cttactgatg gttctactga gtgacctttg gtcgcccaga aatcaagcca
     6781 catttggcgt acttgtgcac tagatagttg tttcatattg tctccttatt cacttgttta
     6841 atgtgattgg ctttccagca tttccacata gtcaatcgcg acacagaggg aaatgactag
     6901 gtctgcataa gcgtcttcaa gaaccgttac ggtataggta gaggtcagat ggaagagttc
     6961 cttcttaatt tccgcaatca actgatcgcg atcatccagc aatttgaaat tcaaatccca
     7021 gatattgccc tcgatacgaa gacctagatt atcaaactca tacttatctc gccagaaggt
     7081 caacttctta cgaatgacaa aactcgagcc atcccgaagc tgaatctcaa aacgaggaag
     7141 caaggtcaag atttctttac taatctcact gacttgttca ccagccgcat catagatggt
     7201 aaaggtttta ggaatcttaa aaaatgatcc ctccacctga taggcaattt ctcccctgtc
     7261 attcttgata gcgaagcgtt cgcctccaag acgaaacttt tgtttgacaa gaaatgtttt
     7321 catcaacacc tccaaaaatc aaaagacaag ctcatatcac gaagggcgaa aaaccgcggt
     7381 accaccttca ttcaatgaac ttgtcattct cttgttctta tgcaattgta tgattgagta
     7441 gcatgacttc ctagcttaga tggctcgcag caccgccatt tctctggact aagacaagtg
     7501 aaaatcaatt ctcaactttc ttattatatc gtttttttaa tcttgcgtca actggaaatg
     7561 atctccgttg aattagacca attccctaca tctctgatta ctttttcagg atatattttt
     7621 tcttactgcc atttttcttt ttatcccaaa ttttcatatt actaaacaca gctactagaa
     7681 tatttccaaa tataaaggtg cctatcaccc aatatatgga ctcagttgtt aggtattgtc
     7741 gatccaagcc atcctttaaa tggaatagta taacagtttg gttaacaatc ataaaggttg
     7801 gccagaaact ttttttgaaa aaagtagaca ttttcattat ttgttgccgc tttctgtaag
     7861 gttaatactc aataaaaatc aaaaagcaaa ctaggaagct agcctcaagc tgtacttgag
     7921 tacggcaagg caacgctgac gtggtttgaa gagtataggc ttagtatact actaggcaag
     7981 caaataagca aataaacaaa taaacaacta gaatagaaaa agatagggct ctaaaaactg
     8041 acttctattc cttaaaaacg aaccagcttg actgattcgt cttcttacgt ttatctccta
     8101 cttccgatac attttaaact gtaggaagag gtcgctatat ttccctgtcc atttatggtc
     8161 aaatttctca taaacttcta ggtgtttcat ggtttcaaca tcgggataga aggccttatc
     8221 ttcctttgtt tcctctggga gcaattcctt cgctggtagg tttggtgttg aatagccgac
     8281 atactccgca ttttggagag cattttcagg tttcaacata aagttgataa aggcataggc
     8341 tgagttttgg tttttaactg ttttgggaat gaccatattg tcaaaccaaa gattgctggc
     8401 ctctgtcggt accacataac gtagattttc atttttttct aacatttggc tggcttcacc
     8461 agagaaggtc acgccgattg caacattatt ctgaatcata tagcccttca tctcgtccgc
     8521 aacgatagcc ttgatatttg gagtcagttt gtagagctta tccactgtct cttccaactg
     8581 ctgcagatcc ttggagttga ggctgtagcc gagggaattg agtcctagtc ccagcacctc
     8641 acgcgcccca tcaaagagca tgatagaatt cttatactcc ggcttccaaa ggtcatccca
     8701 atgctcaggc gcttcatcta ccatggtttc gttgtagaca attcctaagg ttccccagaa
     8761 gtaagggatg gagaatttat tacctgggtc aaaggactgg ttgagaaact ctggtccgat
     8821 attttcgatt cctttaattt ttgaataatc aagcggaacc aagaggtctt cgtccttcat
     8881 cttgttaatc atgtattcac ttggaatggc aatatcgtag gtcgttccac cctgctttat
     8941 cttagtgtac atggcttcgt tggagtcaaa agtctcgtac tgaacttgaa ttcctgtttc
     9001 ttctgtaaac tgagtcaaga gttcaggatc gatatagtct ccccagttat agataaccaa
     9061 tttttgacta tctcgactat tgattttact atctaaatga gtcgcaattc cccacaagac
     9121 aaggataatc gctgcaattc ctgctaaaaa tgaatagatt tttttcatgc ttgctcctcc
     9181 ttctcacgag agataaagta ataacctaca actaggataa tactaaagag aaagactaga
     9241 gcagacaggg cattgatttc taaggaaatc cccttgcgag cacgagagta aatctcgact
     9301 gatagggttg aaaagccatt tcctgttaca aagaaggtca cggcaaaatc atccagcgaa
     9361 taggtgaagg ccatgaagta acctgcaatg atagacggag tcaggtaagg aagcatgatt
     9421 tccttaaaca tctgaaattg actagctccc aagtcatagg ccgcatgaat catgtcgcca
     9481 ttcatttcct tgagtcgagg caagaccatc aagaccacga taggaatgga gaaggccacg
     9541 tgactagata gaacggtcaa aaagccaagt gaaaacttga gttgggtaaa gagaatcaag
     9601 aagctagcac caatcataac gtcaggcgca accatgagga tattattgag tgatagaaag
     9661 gcttcttggt atttcttacg agactggtag atgtaaatgg caccaaaagt cccgataatg
     9721 gtcgctatca aggctgatag gaaggccaag aaaaatgtct gagccaaaat cagcatgagt
     9781 ctcccatctc caaacatggt ttcaaagtga gtccagctaa aacctgtaaa gctattcata
     9841 tcatcaccag cattaaaggc atagccaatc aagtaaaaaa tcggcaggta gaggaccaga
     9901 aagaccagtc ccagataaag gttggcaaat tttttcatcg ttctctcctt tccttggtca
     9961 cccacatggt gatgaacatg gtcaggatga gaatcacacc gatggttgaa cccataccat
    10021 agttgtcatt ggttagaaaa ttctgctcaa tagccgtccc caaggtgata acgcggttcc
    10081 caccaatcaa acgggtcagc atgaagagac tcaaacttgg gataaagacc gactgaaccc
    10141 cacttcgcac accgttcata gatagaggga agatgacatg acggaaggtc tcccacttag
    10201 ttgcaccaag gtcgtaacta gcattgatga gattattatc catatcgtct aagacattga
    10261 aaatcggcaa aatcataaag gggagctcga tgtagcttgc gacaaagata aaggaaaaat
    10321 cggtaaaaag caactgttgt gaaccaattc cgataaattc caagaattgg ttaatagagc
    10381 cattttgacc aaaaatcccg ataaaagcat aggctttaag gagcaaattg atccaggtag
    10441 gaaggataat cagcatgagc cagagttgac ggtgcttgag acgggtcaaa aagagggctg
    10501 ttggatagct gataagcaag gtcacaaagg tcacaatccc tgcataaagc actgagttga
    10561 aactcatttt gaggtaggtc aagttttgtg acgcaaagta agatttataa ttttctaaac
    10621 taaactggcc ttcaatgttg aaaaaggatt gtccgaaaat caagaccaag ggtgccaata
    10681 caaagagggc aatccaaagc atgtagggca ctacaaagag tttagagctt gttttcttca
    10741 tctctttcct cctcgatcgc attgatcaga cctgcttctt gctcttcgat ttccacgtat
    10801 tcttcgatac gagcatcgaa ctcttcttcg gtttcattga gacgcatgat gtggatgtct
    10861 tctgattcaa agtccagacc gatttcctca cccacgatgg ccttacgggt tgagtggatc
    10921 atccattcat ttccaagttc gtcataggcg ataatttcat aatgaactcc acggaagagc
    10981 tgggtgtcga ccttaacttg gagtttgcct tcttcaggaa gggtaatgcg caagtcctct
    11041 ggacgaataa cgacctcaac aggttcattt ggcttcatcc caccatcaac cgcttcaaag
    11101 cgtttgccgt taaattcgac caagtagtcc tcaatcatgg tacctggcaa gatgtttgac
    11161 tccccgataa aggtggcaac aaagtggttg attggctcat cgtagatgtc cacaggggtt
    11221 ccagactgga caatctcgcc atcattcata acgaaaatcc agtcactcat ggcaagagct
    11281 tcttcctgat cgtgagtgac aaagacaaag gtaatgccca atcgttgttg taattcacgc
    11341 aattcgtact gcatgtctgt tctcaatttc aagtccagcg ctgataaagg ctcgtccaac
    11401 aagaccacac ggggttggtt gatgatagca cgggcgatgg ccacacgctg acgttgtcct
    11461 ccagaaagtt tgcggatgga acgtttttca taaccttcca actgaaccat cttgagaact
    11521 tccgctacac gctgctcgat ttctttctta tcaattttac gcaagcgaag tggaaaggca
    11581 acattttcaa acacattcat atgtgggaac aaggcatagg attggaagac ggtatgtacg
    11641 tcgcgcttgt tggttggaat atcattgata cgaacaccgt ctagcatgat atctcctgtc
    11701 gtcgcatcca gtaaacctgc aataatgttt aggatagttg atttccccga accagatgca
    11761 cctagaaggg tgtagaattt cccttcttcc aactcaaagt tgatgtcttt gagaaccttg
    11821 gtgttgctgt cttcaaaaac tttagagacg tttttgaatt cgataattgg ttttttcaat
    11881 tggcataaat tccttctttt tcatagatta accgatcggg gctctgtcag gtccccacta
    11941 cctcttgcag ggagtaaaac cacctgcata catcttcgct accgataggc tttcaccccc
    12001 tttccatgac atagcagcag cttttcaaat acagcttcct acttgctttc acccaagatc
    12061 cggacttctc tttcaagcgt aatacctgag tgttccttga ctttttcgat aaccgattgg
    12121 atcaagtcct cgtagtcttt ggccgttcca tctgcgacat tgatcataaa tcctgcatgc
    12181 ttttctgaca cttctacgcc accgatacga tagcctttca agccagcttc tgaaattaac
    12241 tgacctgcaa aatgcccgac tggacgctta aagaccgagc cacaagatgg gtattccaaa
    12301 ggttgcttga gttcacgtag gtgcgtcaag cggtccattt cctgcttgat aacctgatgg
    12361 gttcctgggg ctagggcaaa tttaactgac aagacaactg caccagactc ctgaatagct
    12421 gaatggcggt aaccaaaagc caagtcttta gcagacaggg tttcgatttc tccatccttg
    12481 gtcaagacct tacaagactg caagatgtga gcaatctcgc caccataggc acccgcattc
    12541 ataaagacag caccgccaac gcttcctgga ataccacaag caaactcaaa gccagtcaaa
    12601 ctatgacgga gggcaatgcg agttgtttca atcaagttag ccccagcttc tgcttcaatg
    12661 gtatagccat caacagaaac gttattgagc ttgtcacaca agatgacaaa tccacgaatc
    12721 ccaccatcac gaacgatgat attgcttgca ttgccaagaa ccatccaagg gatattttct
    12781 tggttggcaa atttcacaag gcgcgccaac tcaaaacgat ttcgtggaaa gaccaaataa
    12841 tcagcctctc cacctacttt tgtataacta tagctatgca agggttcctt aaaacggata
    12901 tcaattcctt ctaagatttc aagcattttt tctcttacag acatgtcact cttcctttta
    12961 caaaattcat tccattatac catttttaga gacatttgac gaccataaaa ataccttgtt
    13021 tggattttgc ataagaaaaa gaggttcccc cctttttatg attttttaca aaagatttcc
    13081 ttggttccat aggcgaccag aacgagctcc agtgctagaa tcacttcaac caagactgga
    13141 tttgtcaacc agcctacttg gaaaagagat ggtgccagat caaagaaggc atgcaagcca
    13201 taggctgcta ggagataaat ccatttcttc tggcgaacag cttggtaaac ccaaactgtc
    13261 aaaagtaatt ggaaaccaag cgccaagatt cgctcaaaac caagcaaata aatctgccag
    13321 accgaaagtg actgaatggt ttttaacata ttttcagaca gtaattgcat aacctgtgga
    13381 ttctgagttt gaactgccga aagaacgatg taaagattga gtaaactaat aaggcctaga
    13441 aaaatcaact ccaagccacc atgccctagg ccataggcca aggcatctgc cttttccaaa
    13501 cttctctttt tctccaacca tttgaagaaa acaagacgag ccgtttcctc aaaaaatgct
    13561 gccatggcta ggccatagat aatatagata agcggatgat cttgcaagag ggcaatacta
    13621 ccgtcttttt gagggtgcaa aatcaagata tgcaccagtt tttccaaaat ctgcgaagag
    13681 acaaagaaag cgactgcacc caagcccaag acagctagat taatctggta tttctttttg
    13741 gcaaaccaaa tgcttcctat cagaaaagct agcaacagca acatggtaat aatgatatga
    13801 atggtcattt tcttctccta ttctgccttt tcaatatctt ttttcatctc gtcaacattg
    13861 aacttagcaa acaagtattg acggtcttgg actgggaaac gttggtccaa ctggtcaact
    13921 gctcccacct cgataatgcc ttccttgatg acaaaatcca tgacatgtcc accgaaggtc
    13981 aaatcatctg atatgaagtg cagatggtag cctgccacac tgaccccatg gaaaatctca
    14041 ggcgtccaga aaccaacaat agtccccgca acattgtcac gactatattc cggttgatgg
    14101 gttgcgacat cagcaaactt agtatcgggt gttgacttag gaatcatacg cacatgcata
    14161 tgtgaaaatt ctccccgaat cttgatagaa cggaaaagat tttccccatc ataataagac
    14221 tcaattcgtt ctttcaattc cttgtctgtc atctcaaagc gctggcggaa aatgacctct
    14281 gcctgatgcg gtaccactgc agcgtaagga ataagggcat ctggtgacac ttccacaatt
    14341 tctggctggt ctcctgaccc tttggcctga taagccttgc catccaagac aatcaattcc
    14401 ccatcaatag aatccaaggt tcccaaacca aggtcaccat gctctagcaa ttctcccact
    14461 gtcatggtac caccataaag gccagccatc aaggctccaa gagtattgta ttgaaataat
    14521 ttcactggtt cctgcacgtt cttatccatt cactttcttg tctgttattc tataaatctt
    14581 actcctaagt ataccacatt tgcccctaga tgtgaacgag agaaacgctc tagacattgc
    14641 caagaaggaa aaaaaagggt acaatgtaac aaaatcaagg gaggtctgga atgaagaaac
    14701 aaagcaagta caaagaggtc gtttcctatc tgaaaaatgg tatcgagtct ggacgatttc
    14761 cgacgggtag tcgcctgcct tctatccgtc aactgagcct tgactttcac tgtagcaagg
    14821 acaccattca acgagccctg ctggaattac ggcacgaaca atacctctat gccaagcccc
    14881 agagtggcta ctatgtccta gaacaagggc aacaccagga cctagaaatc gaggttaccg
    14941 acgaacatgc cagtgcctat gacgatttcc gactctgtgt caatgaaacc ttgattggcc
    15001 gagaaaacta cctcttcaac tactatgata atcaagaagg attagaagac ctaagacaat
    15061 ccattcacaa actcctcttt gagcaagctc tctactgcaa ggctaaccaa ctagtactga
    15121 cttctggaac ccaacaagcc ttgtttatcc tctctcaaat atcctttcct agccaagcca
    15181 aggaaatctt ggtggaacag ccaacctacc atcggatgaa tcgcctcttg attgcacagg
    15241 ggctggacta tcaaacgatt gaacgaggca ttgatgggat tgacctgaag gagctggagc
    15301 gacacttcaa gacaggaaaa attaagtttt tctacaccat tccccgattt cactatcctc
    15361 tgggacattc ctattctgag caagacaaac gatccattct taacttagct gccaagtatg
    15421 atgtctatat cgtagaagac gattatctgg gtgatttgga ctccaagaag ggccaaacct
    15481 tccactatct tgatacagag gagcatgtca tttatatcaa gtccttctcg accagccttt
    15541 ttcctgccct tcgtattaca gcactcattc ttccaaatgc tatcaaagaa gcatttgtgg
    15601 cctacaaaaa tatcctagac tacgacagca acctcattat gcaaaaggcc ctgtcactct
    15661 atattgacag tcaattgttt gaaaaaaatc gtttggctcg cttgaccaat catgaatctt
    15721 accaaaaaca aatcgaggaa aggataacta aaacaccttg tccccttcct cattatcccc
    15781 tacacgatgg tttattgcta gacctgagac agtatcctaa aatcgccagt ctcaaacaca
    15841 gtcaaccggg cttggacttc tttgaagagg cctatttaag tacctgtcct tatcaatttg
    15901 ccaaggtgtc cctagacaat ctggaaaatg ttttaaacta tttaaaagca gaattggaat
    15961 gacatccaac tctgcttttt cttattcttc aacttcagtt agacttgctg ctttttcaag
    16021 atagctttgg taaattcctt catccttaag tttttggatg accttgtcta ccacttcttt
    16081 caaatcatca ctattttttc taagagcaac ggcattggcg tcgccgtcct tcatcttcaa
    16141 gctgacagtt gcgacagcta agccagcgtt tttagcagca taactaagtg caacaggctc
    16201 atccatatga acagcatcta cttttccagc ctgcaattca ttgactgctt cacccatatt
    16261 agttagggaa gttaattgag cttttggcaa ttgttccttg accattgatt ctggaacagt
    16321 ccctttttgg gctgcaatat tagcactttc taggctagtt aaatccttgt atttttccac
    16381 atcagcctta cgaaccaaga aactaatctt gttttcatag tatgggattg aaaaatcaaa
    16441 gacttctttt ctctcgtcag tagcactaat tcctgcaact gctaggtcag ccttaccagt
    16501 ttgaagactg gtcaaaacat tgtcaaaact catgcttgag atttccaact taaccccaag
    16561 ttcatcagcg atagcctgag ccatgtcgat gtctgcaccg actacctggt tctttccatc
    16621 aaccaatgat tgaaattcaa agggtgcata gtcaggactg gttgccacaa ctaatttccc
    16681 cttttgctta attgcctcaa cagctgactg agaaccatta gaacctgact ggcaagctac
    16741 taagaagaaa cttgcaagaa aattacataa aacaaatatc catttttttg atttcataaa
    16801 taaacaacct ctctgttaat tttgtataat tataacgata tccaagttac ttgtcaagtg
    16861 ttttttaaat ttttatctca aaaatatttt ttcgttcaaa aaaaggagcc atcagttgat
    16921 ttcaagctcc tttttataca gaattaaact attttatagt tcgacaatct tacctgtttc
    16981 aaagtagaca acccattcac agatattttt agcatagtca ccgatacgct ccaagtagga
    17041 aataacttgg aaataatcac gacccgtaac aatggcttct ggatttttct taatctcttc
    17101 agtcgcaagg tcacggatag tttcaaaata gtggttaatt tgctcatcca tggaggccac
    17161 ccggtatgcg tcgtcaacag aaccattaag ataaagatca agtgctgctt caacaacgct
    17221 tttaacttca cgtcccattt ttttaatttc ttcctctaca gctggaatac gttcttcccc
    17281 cttcatacgg atggttgcct gggcaatggc tacagcgtga tcccccatac gctccacatc
    17341 tgatacagcc ttaaggacag tcaagactgt acgcaaatct tgagagactg gttgttggag
    17401 tgcgatcatt tcaaatgatt tcttttccag tttcacttcg tattcattta cttctgcatc
    17461 atcttcgatg acctcttttg ccaggtcacg gtcatgcgtg acaaaagcac gtaccgtacg
    17521 attgatttgt gagagcactt cttgtcccat agcgtagaac tggttatgta atttctctaa
    17581 atcttcttca aattgagatc gtaacatctt tcatctcctt atccaaattt tcctgtaata
    17641 tagtcttccg tttccttgtg ttggggatca aggaacatct gcttggtatc attaaattca
    17701 atcaaatctc catctaggaa aaatcctgtc ttatcagaga tacgtgaagc ttgctgcatg
    17761 gaacgggtta ccagaagcat ggtgtacttg tcttttagac catacaaggt ttcctcaatt
    17821 ttaccagctg aaatcggatc caaagccgaa gttggctcat ccaagaggat gattttagga
    17881 ctagttgcca agacacgggc cacgcagaca cgctgctgtt gaccacctga caatccaata
    17941 gctgaatcat atagacgatc cttgacctca tcccagatag aggcaccttg caaggctttt
    18001 tctacggctt catccagaac ctgcttatcc ttaattccat tgatacgaag cccgtagaca
    18061 acattctcat agatagtcat agggaaagga ttaggttgtt ggaaaaccat tccgatttcc
    18121 ttacgtaatt caaccgtatc tgtacgcgga ctgtagatgt tgtgaccatt gtacaccacg
    18181 gatccagttg tggtcacctc tggattgaga tctcccatgc ggttgagaga cttgaggagg
    18241 gttgacttcc ctgatccaga tggaccaatc aaggctgtaa tttccttagg ttggaaagat
    18301 agggaaacac tattcaaagc cttcttttta ttataataaa cggacaggtc tgatacctgt
    18361 aaaatcgcat ctgtcatacg gtttcctttc taaccaaagt gaccagatac atagtcattg
    18421 gtggactgta gcttggcatt ttggaaaata gttgcagtct tgtcatactc aatcaaatca
    18481 cccaagtaaa agaagcctgt atagtcactt gcacgagcag cctgctgcat attatgcgtt
    18541 acaatgatga tggtaaagtt tttcttgagc tcaaacatgg tctcttctag ttgcatggtc
    18601 gcaatcggat ccaaggctga ggctggctca tccattaaga ggatatctgg cttaacagag
    18661 atggcacgag cgatacagag acgttgttgc tgaccacctg ataaggtcaa ggctgacttg
    18721 tggagatcgt ctttaacctg atcccagagg gcagcctgac gaagggaggt ttctacgatt
    18781 tcatctagga cttgcttatc cttaactcca gcacgttcat gcgcaaaggt aatattacgg
    18841 taaattgact tagcaaatgg attggggcgt tgaaaaacca ttccaatgtg tttacgcatt
    18901 tcataaacgt tgatttctgg acggttgaca tcaattccac gatagagaat ctgcccagtt
    18961 actttagcaa tatcaatggt atcattcatg cgattgagac tgcgtaagta ggtagatttc
    19021 cccgatcccg acggaccaat caaagctgta attttatttc tttcaaattg catatcaatc
    19081 cccttaatgg attcattttt accatagtaa acatggacat ccttagtaga aagggctact
    19141 ttttcttcag gaaaggtaag gatatgcttc tcatcccagt tatatgttga catggcttct
    19201 cctttaggca gcggttaatt tcttgtgtag atagcttccg aacttacgag ctccaaagtt
    19261 aaaaatcagg ataaagatca ggagcacagc ggcagaacct gctgatacaa tggttccatc
    19321 tggaatagtg ccttcactat tgactttcca gatatggaca gccaaggttt ctgcttgacg
    19381 gaagatagag atggggctag tcacactgag gatattccag ttagaccagt caagagctgg
    19441 cgccgattgc cctgctgtat agatcagagc tgcagcttcg ccaaagatac gaccagatgc
    19501 caagacgaca cccgttacaa tacctggaag cgcttctgga ataacaacat gaaccactgt
    19561 ctcccagcga gaaatcccaa gagccagacc agcctcacgt tgggtatggt gaacgtgttt
    19621 caaactatcc tctacattac gcgtcatctg aggcaagtta aagactgtca aggccaaggc
    19681 acctgaaatg attgaaaacc catactcaaa ctggactaca aagatcaagt aaccaaagag
    19741 acccaccacc actgatggta aagaggacaa gatttcaata caagtccgca caaagttggt
    19801 aacaggacct tttttagcat attcagccaa gtaaatccca gctcccatag aaagaggtac
    19861 agaaataatc aaggtaatga ccaataggaa aaaggaattg taaagctgaa tgccaatccc
    19921 accacctgct tgaaaagcag aagaccttcc agtcaagaaa gaccaagaga tatggggcaa
    19981 gccccgaacc aagatataga gaattaagga agccaagatt gtcacaatga tgctagcaat
    20041 cgtatagagg acagctgttg caagtttatc taatttctta gcgcgcataa tttttctttc
    20101 ctctttcttt cgtaatcaat ttaatcacac tgttaaaaac taaactcatc aagagcagta
    20161 ccaaggccag tgaccagaga acattattat ttacagttcc catgacagtg ttcccaattc
    20221 ccatagttaa tatagaagtt aaagttgcag ctggtgtggt caaggaagtt gggataacag
    20281 ctgagtttcc gacaaccatc tggatagcta aagcctcacc aaaggcacgc gccatcccaa
    20341 agaccactgc agtgaaaata ccagaacggg ccgccttcaa gatcacacgc cagatagtct
    20401 gccagcgagt ggctcccata gcgaaactgg cctcacgata ataacgagga accgcacgca
    20461 agctatccgt tgtcataaag gttacggtcg gcaaaatcat gacaaagagg acggaaatcc
    20521 ctgacaaaat cccaaaacca gtcccaccaa agacactgcg aacaaaggga acgacgactt
    20581 gcaagccaat aaatccgtac actactgaag gaatcccaac caggagttca atagctggtt
    20641 gcaaaatctt cgcccctttt ggtgatactt cggtcataaa aactgctgca ccaatagcaa
    20701 agggtgttgc gataagggct gagagaatgg taacgataaa ggaacccaaa atcataggaa
    20761 gggcaccaaa ttctttacta gaaggattcc aagttcctcc caaaagaaag tcaaagatat
    20821 tcacaccatt gacaaagaag gtcgacaagc ctttttgcgc tacgaaaacc aaaatcatgg
    20881 ccacaaggat gactatcaaa gaaagacagg caaaggtcaa accttttcct aatttctcca
    20941 gacgagaatt ctttgatgga agcaacattt tcttagctaa ttcttcttga ttcattattg
    21001 tctcccttcc aacactgtca cagttccggc agcatctttt tcaaccttca tttccttaat
    21061 cggaatatac ttcaatcctt tgacaatccc ttcttgggtc tcatccgaga gaacaaaatt
    21121 gagaaattct gcagccaact cattgggctg ccccaatgta tacatatgct cataagacca
    21181 caagggccaa ttattgctac ttatattttc tggacttaag tcatagccat tcaacttcat
    21241 gcttttgacc gaatcatcta tataggtaag agataaataa gagatagctc ctggactttt
    21301 tgatacgatt gattttaccg ctccatttga atcctgctcc tgactttgca tggcagactg
    21361 accttccata atgacagtat caaaggtagc acgagaacca gagccggctg cccgattgat
    21421 aacagagatg ggtaagtcct taccaccaac ctctttccaa ttggttacct cacctatgaa
    21481 gatttgacga agttgctctg tcgttaggtt atcaacatca acctccttat tgacaatcag
    21541 agccaagcca gctaccgcga ccttgtggtc aacaagagca gaagcatcaa ttccgtcttt
    21601 ttcctcagca aatacatctg agtttcctat atcaactgcc ccagactgaa cctgggacaa
    21661 gcctgtacca gaacctcccc cttggacatt gaccgttttt ccaacatgga tcgtgccaaa
    21721 ttcatctgcc gctacttcaa ccaagggttg caaggcagtt gagccaacag ccgttatgga
    21781 ttctccacga tcaatccagc tagcacagcc tactaaacaa gccgtcagcc aaaaagcgat
    21841 aagagacaga gcaagctttt ttcttttttt cactgttttt ctcctcgaaa ataattatga
    21901 atactgtgaa ttttttaagt agttctttat gagttgacgc atgaattctt accaaatttc
    21961 tgcgcaattg attatttata taatataggc tatattactc tttcctaacc tccttttttc
    22021 atatgtggat aaaatctctt gtctatccct tcccccattg tcacccatta tagtcatttc
    22081 gtgtctcttt ttcccctttt taatgcaagg gaaattactc tccttagatg ataatccaaa
    22141 agctagaaag gtatctcaaa cctctctact ctcccagact agtttacaac taaaaggaaa
    22201 agattctatt ttatgagaaa tctagtttac aagcggtaag aacgctaata actaaacttc
    22261 ttgtactctt tgaaaatctc ttcaaaccag tgttttgagc tatctatggc tagcttccta
    22321 gtttgctctt tgattttcat tgagtagtaa aactacatgt aatggcaatc aagatatcaa
    22381 gaatcatcct actaaaaaaa tccatacttt cactataaca tagaataaga tatttgacta
    22441 gcattttcat ttgaatctga ggccttttgg aaaataattt ttcaaaacat ttccagtaac
    22501 ctttgcaaag cccaagccat tgcctttaac caaaacttgg taccaaccat ttggcagact
    22561 ttctgccagc tgaacgattt ctccagccgc atacttgaca aacgcttctt ggccaatttc
    22621 aaccgactgt tcgacctgac tcggtttcaa ggctaaacca agagcgaaac tgggctcaaa
    22681 gcgtttcttc ttaaaagtgc ccagatgcag tccattgcga gcaatcttga gcttccctaa
    22741 atctggcaaa agttctggca agagataaag ctggtctcca aaagtctgca agatacccgg
    22801 tagattgacc ttcaaatggt tttgggcaaa ttcctgccac aaggcaactt gttcacggct
    22861 gaggttactc ttacttgcct taaatttagg agctggattg ttacccttaa actgtagatg
    22921 ggcaacaaac tgaccctctc ccttaaactg atgagggtac atccgagccg tttctggcag
    22981 gtcaatacca gctaccattc cattgatatg ctctactggc aacaagtcaa aatcatactc
    23041 ttccagcaac caattgacaa tctcttcgtt ttcctcgggt gcccaggtac aggtcgaata
    23101 aaccagacga ccaccttcag ctaacatggt cactgcatcc tccagaattt ctctttgcaa
    23161 gctagcacat tgactcggat aatctaagct ccaatagtcc atagcatcag gttgcttacg
    23221 aaacattcct tcaccagagc aaggggcatc aagaacgatt aagtcaaaat agcctttaaa
    23281 gaccttgacc aagcggtcgg cagattcatt ggtcaccacg acatttgtcg ctccaaaacg
    23341 ctccatgttt tcaaccaaaa tcttagcccg tttgcttgaa atttcattgg aaacaagtag
    23401 cccctcccct gctagatagg ctgccagttg agttgatttg ccccccggtg cagcagccaa
    23461 gtccaagacc ttcataccag gactgggttg agctacttga gccaccattt gagcagcagg
    23521 ttcttgcgaa taaactaaac ctgtagcatg ctcaggcgat ttccctgaaa ccttcccata
    23581 gtggccccaa ggggtttgag taatggcatc agaaaaggaa agttgctctt cttttaaggg
    23641 attgacccga aaggccgaaa ccgcttcctc ctcaaaagag gcaagaaaat ctcttgcctc
    23701 atctcctagt atctctttat atttttcaac aaatccttct ggaaattgca tttaagttct
    23761 tttcctttcg taaatatagg actgaatttc ctcctgcatc tcaagaggca ccatcatgac
    23821 cggctgtctg gtttgaaaat caggagcttc accaaaaagg gtcacaaccc gatagcccag
    23881 actttcccct aaaatactag ctgcggcata atcccatggt tgcagataag tgagataggt
    23941 caacaaacgc cctgacaaaa tcttggcaaa actaatggcc gcacttccat agacacgaac
    24001 accaagaacc gctcggctca aatcagccag cccccattca ttggtttcca gcataccact
    24061 attccctgca atgagaaaat ctccaagtgg tttagtttta aaaggagcta ggggcctatc
    24121 atttagacaa actggaaatt ccccaccacc gtggtaacaa tcccctttga ccacatcata
    24181 aatcagacca aactgtccct gaccattttc aaaataagcc atcataacag caaaatcttc
    24241 ctgctgggct acaaaattat tggtaccatc aatgggatca atgacccaaa ccttgccctc
    24301 ttgaaccgag gctcgcagac aaccttcttc agcacaaatc ttatcctcag gataacggga
    24361 caaaatctca ccaaccaaga gttcctgaac ttctttgtcc agtctggtca ccaaatctgt
    24421 tggagaggac ttggtttcaa cacgcaagtc ttcctgcata tggtcaagaa tgtactgacc
    24481 tgctttctta acaagctctt tagcaaattc aaatttactt tccaagagaa atctttcctt
    24541 cccctttttc tttggcagcc tgaactgctc ggtaaagtga atagccacta accgtttcaa
    24601 attctcgtcc caaacgtttc tcttcgctct tgcttggcac gaccgctttg aatcccttat
    24661 aggagtccag taacttttta gcctctactt tgccttcata ggcagcttca acatcattaa
    24721 aaaaagaaag cactgaagca agttcttcag tgctccacga caaatctagt gggtaactat
    24781 actgtttgtt cattaactaa taccagctct cattcttgct tcttttagtt cttgcttacg
    24841 ataactacga gggagaaaag cacgaatctc atcttcatta aaaccgattt gcatacgctt
    24901 ggcatcaata ataattggac gacgcaaaag actaggatac tgctcaatca aatgaagcaa
    24961 ttccgatacc gaaatactct ctacatcaat attcaatttt tgaaaaattt ttgaacgagt
    25021 tgaaatgatg tcatcagtac cattttcggt caaggaaagg atgtgttgca attcttttct
    25081 tgttaaagga ctggtcataa tattgtgttc cacaaaggga accttatgtt tttctaacca
    25141 ggccttagcc ttacgacatg atgtacagct cggtgataga aatagtgtaa tcatgctttt
    25201 ctcttcttat ctatactttg ctacttctat tatacaaaaa aataaggcgc ttgactaggg
    25261 atttttagaa aaaaagccta ttttttcaag aaaaataggc tgtttgcgaa cgattgacac
    25321 aattggattt ggttaattca ctcttaacga tggttttaaa cgatatatat ttttatatat
    25381 gcaaattaaa aacatctttc atttcacttc ctacgacttt tcagacacag atagccaaag
    25441 aagctttcat agagggcaaa aaagaggagg aaggcatgaa gaaagaaggt ctctggcaaa
    25501 atcataataa caggatcctt ggctggatca aaaagccagg tatcatctcc cacaaagaga
    25561 atttgatgga aaagagtaaa gaattggtca aaaccaatca aaactccccc aagtccaatc
    25621 atcacaggta agactactag agccaggaga ctttttcgat aaagagacaa aaagtccttt
    25681 ttcacaatcc tattgacaaa gacatagaaa cttggcagtg tcactagagc tactagctga
    25741 accaaatgaa agagattctt gaccactgcg aaatggtgca gaccagctgc tgacgaacga
    25801 aaatcaggca tctgtaagac ctgactaaaa ggattggtca gataattcat caagatatga
    25861 aaattgtatt gaatggtttc tggttttaga tagactcgat tcgttaagtt tagccactga
    25921 atctccatag gatagaaaat ccaagccaga taaatggtca gaaggattga gagggagagg
    25981 agaaagagca tagagcccca aaaggtcaat ttagttttca tcaaaatccc actccgcaag
    26041 gctagaaacc acatgtgtcg gtgcgattgg caggccagct acttcttctg ccttggtaaa
    26101 acctgtcgtc accaagagcg ttggaatgcc attgtcaatc ccagcccgga tatcagtcaa
    26161 ataattgtcc ccaaccatga ttaactcttc acgttccaaa cctaagtgct caaccgcctt
    26221 gtccataatg atggcatttg gttttccgat ataaaccggc ttcactcgtg tcgctacttc
    26281 aagcagcgta atcagtgagc cagcacctgg caaaagaccg cgttccgtcg ggatgttgag
    26341 gtcaggattg gttccgataa aatgggcacc cttttgaata gcaagagttg ctgtggcaaa
    26401 tttttcatag tcgacttgcc aatccagacc aactaccacg taggcaggtt tttccttgtc
    26461 ttccacataa ccagccgcct tgatggcttc cttgagtcct gcttctccga cgacatagac
    26521 ggtcttttca agccccaaat cattcatata gtcgatggtt gccaaagtcg ctgtgtagac
    26581 agtcgatagg ggcgtatcga tattaaaatt ctgagccaac atctccttaa cactctctgg
    26641 agtgcgggtt gtattgttgg ttacaaagag atagggaatg tcccgctttt gcaattcatg
    26701 aacaaaagtc tctccagcag ggattcggtc tttcccctta taaatggttc cgtctaaatc
    26761 aattaaatag cctttatatt tcatctattt ctccctaagc cttttttatt tcttgccaag
    26821 taatgattgc ttgggcattg ataaccccat cacttgtaat ttcatgcttg ctttccagtc
    26881 cagtccgttc aacagccgat gtaatcaccc cacctggtcg aacttccttg acatacttga
    26941 ggttgatttt cttgggaata tagtgggtca aaaaatccgc tcccatgacc tcaaaaatcc
    27001 agtccaagta tttactgtta ttgacatgac cattcatatc caagtcgtaa aaacgaacat
    27061 ggtaatcctt gctgatcggt tcttccaagg actcatactt cggtccacgg ataagttttt
    27121 tatcaaaatc agactggtaa ggagccacaa tctcaggttc aacaacatgg acttttcgac
    27181 tgtcgcggtc catgagaaca aaggtcgcca tcatgtggat gagctcctgc tccgcttcat
    27241 tataaatagt aaagcgacgg tagcaaaaaa gtcgattgta gctcaaggct tccgtttcga
    27301 tggtaatttc ttccgcaaaa cgaggcaaac gaaccacctc aatatcatat tctacgataa
    27361 tccagaccag attatattct tccaaaatgg ccttatcact aactcccagt tcaatcgact
    27421 gcatccctga aacttgcagt gacagcaaaa tcacatctgg aagtttgata tgaccgttca
    27481 tatcagccat atcaaaagga attttcattt tcatttgata agttaagccc atgatcctac
    27541 tccaaaataa atcgttctgc tacagtatct cccaaaaaga gacctctctt tgtcatgcga
    27601 acgtggtcac cctcaatctg catgaggcct tgttgaacca aatctctgac aatttctcca
    27661 taaagtccag caaaagactg tccaaatttt tcctcaaatc gcgccatgga aaccccggat
    27721 ttcttgcgga gtcccaagaa catttcttct tccatttgct ccttttgact caggtgatct
    27781 tctgtaatac aagcattgcc ttcctcaacc gcactgagat aatgacgaat gggaccatga
    27841 tttttatagc gtactccatt gacataacca gatgcccctg caccaatacc atagtattca
    27901 gcattgtccc agtacatgag attgtgacga ctttcaaaac cgggtttgga gaaattagaa
    27961 atctcataat gctcaaaacc cgctcgctcc agctctgcaa tgatgtactc aaacatctcc
    28021 gcttctagtt cctccttagg cagaggcaat ttcccacgtc gcatccggtt cataaagacc
    28081 gtatggtttt ctaaaatcaa actatacaaa ctcatgtggg gaatatccag tccaatggct
    28141 ttagccacat tttcctttac ttgctccatg gtctgaccag gcagagcata aatcaaatca
    28201 atggagatgt tgtcaaaacc agccagtttc agacgatcga tattttcata aatatccttc
    28261 tccaaatgac tgcgcccaat ctttttcaac atcttatcat caaaggtctg gacacctagc
    28321 gaaacacgat tgacagccga atttttcaaa acagctatct tatccgcatc caaatcgcct
    28381 ggattggctt caatggtcaa ctcttccaag acagacaagt ccaagttttt agtcaagcca
    28441 ttcagtaaca cctccagttg cggagccgac agggctgtcg gtgttccacc accgatataa
    28501 agggttgaca acttttcaat atcataagaa cgaaactctt ccagcagatg ctctaaatag
    28561 ctgtcgactg gctgattttt gatgaagacc tttgaaaaat cacaataata acaaatctgg
    28621 gtacaaaatg ggatgtgcac ataggctgac gttggttttt tctgcatagt aattattata
    28681 ccacaaagac tagattccag ataaaaatca ccatccccag atacatagtc cgtccggaga
    28741 tggtgatggt ttattcttct gttatatcaa tcacaatctc ttctgagtca tcaagagctt
    28801 cggctttttc ttgccattgt tccttgagat tatttaattg attttttgat gcttctgtcg
    28861 cttgaaaagc ataggattta gcttgagcaa gtatactgtc cacagtgatt tcacctgact
    28921 caacctgttc ttttgttttc agaacaaaat ctgtagcctg ctccttaact tctgtcagtt
    28981 tttcacagac ttgctccttg gcatactccg gatcttctct caaatcatct agaaaatctt
    29041 gagcctgact gcaaacttgt ttgcccttat cacttgttaa aaacaaggca agagctgcac
    29101 ctgaaaccgt tcctaaaagg attgaggata atttacccat aaggattctc cttttttatt
    29161 ttttgaaaaa tttacttgca agacgaagag ctgacagact tgcaccagtc ttgagtgttt
    29221 ttgaaccagc tgatgaagct ttcttgctca agacacgcgc atggtcattg aggtctgaaa
    29281 cagatagaga taaatctgca acagcactga agagtggatc aatcgtagcc accttgacat
    29341 tgatatcatc tgccaagaca ttgaccttag ccaacaactc attggtgtga tgcaaggtca
    29401 catccacatc tgaagtcaag gttttaatcg tcttttctgt ttcatcgatg acacgaccaa
    29461 gcttttgtac agtaatgatc agatagacca aaaagacaat caaagctagg gcaacaagaa
    29521 tatatgcaac ttctaacatt tatttttcct cctctgtaat atagtaaggg gccttctttc
    29581 gattttgata aataacgatc attataccga gaccgataag gacaactgac agccattggg
    29641 acactcgaag gctgaagaac atgagactat ctgttcgcat accttcgatg accatacgac
    29701 cgaaaccata ccaaatcaag taaaaggccg tgatatgacc tcgtctgaga ctcttccatt
    29761 tccgtctaaa aatcagaatc aaagcaaagc caagcagatt ccatagagac tcataaagga
    29821 aagtcggttg acggtagctc ccctcaatat acatctggtc acggataaag ccaggtagat
    29881 aatccagatt atccactgtt gcaccataag cttcttggtt aaagaaatta ccccaacgcc
    29941 ccaaactttg agcaatcata acgctaggcg ccgcaatatc tagaaaatcc caagtattga
    30001 tgagtttacg gtcagcaaag atatagagca caagagcccc agttatcaaa ccaccgtaaa
    30061 tggccaaacc accattccaa atggcaaaaa tctctcctaa attctgacta tagtaatcaa
    30121 atcggaaaat aacatagtag agacgagctc ctaaaatagc caagggaaag gctactaaga
    30181 taaaatctaa aatatcgtct ggtatgatct tctttctagg tgcttctttc atggtcaaat
    30241 aaaccgcaag aatcaagcct gtcacaatac ataaggcata ccaacgaatg gctaggggtc
    30301 ctagttgaat agcaattgga tcaagcattt tgcacctcat ttcgagcaat gagacttgtc
    30361 agtcgttcgt cgaacaaacg ggtcgcatca aagcccattt ccttggcacg ataattcatg
    30421 gcagctgcct caatcacaac agagatatta cgacctgttt taactggaat acgaatacga
    30481 ggaatggcta cgccagaaac ttcaagttcc tctgcattgt ttccaagacg atcaaaggtc
    30541 ttatgtgtat cgtaattttc caaatagaca gcaagctgaa cctgtgaaga atccttgaca
    30601 gcactcgcac cgtagagact cataacatcg ataataccaa ccccacgaat ttcaatcaag
    30661 tgtttcaaaa tttcagctgg ttcaccccag agagtaatct catccttggc aaagatatcg
    30721 acacggtcat cggctaccaa acggtgacca cgtttgacaa gctcaagacc tgtctcgctc
    30781 ttaccaattc cactatctcc ctgaatcaag acgcccatcc cataaatatc catcaagaca
    30841 ccgtgcacac tggtacgttc tgccaaacga gaatccagat agctagataa ctctccagat
    30901 aaacgactgg tagctgcacg gctggttaaa atagcaatct tacattctct agcagccttt
    30961 aacatctcct ctggaaccac caaaccacga gcaacaatga ctgctggtgt ctcaggtaga
    31021 aacatttttt tcaaaacttc ataacggctg ttggaaggca tgctgatcag ataagaccac
    31081 tccttcatcc ccaaaagttg aatccgctct ggtgtatagt agtcaaaata gcctgtcatt
    31141 tcaagaccag gtcgcgtaat atccgctgta ttgatttcct tttcaagcaa ctctggttca
    31201 ccatagacaa tgtctagtct aagcttttca atcacttctt ttactaaaac cgacatcatt
    31261 tcctccttca aatgagttct tctctctatt atatcaaatt gtaggaggaa gtttcagatt
    31321 tttgcaggct ttggagtatt tatttaaaag aaaaaggact agatttctct agcctttcat
    31381 ttcaattaga attttttacg tttacgagct acttctgatt tacgtttacg ttttacagaa
    31441 ggtttttcat agaattcacg tttgcgtgtt tcttgaagag taccagcttt agtaaccgca
    31501 cgtttgaaac ggcgaagtgc gtcgtcaaga gattcattct tacgtactac tgttttagac
    31561 atttttttca ctcccttcaa gttcaatatc tatttcattc tacacgtaaa aggaataatt
    31621 gtcaagcaaa aatgcggttt aattttgatt ttttcttcca tttatacaag agttgaaagc
    31681 gttcaataga acattgcttt tttatttttc gagtaagcta agcgcttcag catctgcgat
    31741 gatggttaca tcagggtgat tttgaaggct acttgcaggt aggttctcag tcactgggcc
    31801 agatactgtt ccggcaatgg cttctgcttt cgactcaccg taagcaaaaa gaataataga
    31861 cttggcatcc aaaatgtttt taatccccat tgaaatagct tgggttggga cgtcttcaat
    31921 cttggcaaag aagcgtgcat tggcttcgat agtagactgg tcaagttcta ctagatgcgt
    31981 ttgactgtca aatggagtac caggctcatt aaagccgata tgcccattgc gaccgattcc
    32041 caagatttgg aggtcaactg gatggtcagc caaaatttgg ttgtagcgtt caacttcagc
    32101 ttcagcattg tccttaacac cacgaggcaa gaaactttct ttaaatggtt tttggttgaa
    32161 caagttttct tgcatgaagt aacgataaga ttgtggattg tccccatcaa ggcccacata
    32221 ctcatcaagg ttgacactgg ttagatttga aaaatcaagg tcactctcaa caatttcctt
    32281 gtaaaattca agtgggctac ttcctgtcgc aagtcctaga gtttgagctc cattagccaa
    32341 tttttccttc aaaatctcaa aagcaacttt tccaccttga acttggtttt caactttaat
    32401 aactttcatc ttttcatcct ccatttctgt ctatatttat tatatggtat agaccaattt
    32461 ttgtcaagtg aaaacgattt aatttctaaa aaaagagcgt ccaaacttga aacgtgttat
    32521 aatggatagg attagaactt agaaagaatg aacaaatata tgaatacagc tgattttgat
    32581 ttccacttgc cagaggaatt gattgcccaa acgccccttg aaaaacgtga tgcctccaaa
    32641 ctcctcatcg tcaaccgtga gacaggagaa atgcaagata aacacttcta ctctattatt
    32701 gatatgctgg aacctggtga tgcccttgtc atgaacgaca cccgagttct ccctgcccgc
    32761 ctctatggtc aaaaagtgga gacaggaggt catgtggaac ttctcctcct taagaacact
    32821 agtggagacg agtgggaagt tctggctaaa cctgccaaac gcctcaaggt cggtactcgt
    32881 atcagctttg gtgatggccg cctcagcgct gtcgttacag aagaattgac ccacggggga
    32941 cgcattgtcc gctttgaata ccaaggaatt ttcctagaag tcttggaaag tctgggagaa
    33001 atgcctctgc caccttatat ccacgaaaaa ttagatgacc gtgaacgtta tcaaaccgtc
    33061 tacgccaagg aaagcggctc tgctgcagca ccgactgctg gtcttcactt caccaaagaa
    33121 ctgctggcag aaatccaagc taagggtgtt catctagtct atctgactct ccatgtcgga
    33181 ctcggaacct ttagacctgt ttctgtggat aatctggacg aacacgaaat gcactcagag
    33241 ttctatcaac tttctgagga agctgctgcc acccttcgct ctgtcaaaga aaatggtggt
    33301 cgcgtcatcg ctgtcggaac cacttctatc cgcaccttgg aaactattgg ttccaagttt
    33361 gatgggcaaa tccaagcaga ttctggttgg accaatatct ttatcaaacc tgggtatgag
    33421 tggaaggtcg tggatgcctt ctcaaccaac ttccatctgc caaaatcaac tctggtcatg
    33481 ttggtttctg cctttgcagg ccgtgaatta gtcttagatg cctaccacca tgccatccaa
    33541 gaacactacc gcttcttcag ttttggtgac gccatgttta tttattgaga aagaatttct
    33601 ctaattcttc taataccaat aaatcgctaa gatattattt caaagaacat ctacaattga
    33661 aactctagct agctgtagaa gacgcctagt acattgaaat taaaatgctt ccccctagct
    33721 tcgaaaatat tgccatagat tgcgttgact ctccaaattg attcatctat attttatttc
    33781 agcttcctat attttcttcg ctgtttgtaa atcaaaatgc aagacacatg agtagcacca
    33841 tatttgttac tcttatctgt cctctcaaga gactattatg agttatttca gaatcattca
    33901 ctactttgac cctgactctc cttagtctca aaatcaaaga cttatactct tcaaaaatct
    33961 cttcaaaccg cgtcaacgtc accttggatt atatatgtga ctgacttcgt cagttctatc
    34021 tacaacctca aagcagtact ttgagcaacc tgcgactagt tttctagttt gctctttgat
    34081 tttcattgag tattaaacaa aaagtgaaca aatctgaatt ctaatgtaca gaagactagg
    34141 cttgttcact tttttatagt cgctataaga tgaccttatc tatagctttt tatatataat
    34201 tatatattca gacatactat tatcaatttt gtcgcaggga ggaatctgtt aacgcaccca
    34261 ttcaccatta tcattgactc tatagccatc tatacttgta ttgaccgcta actcacccga
    34321 tgtatttaca taataccatt taccaccaac ttggaaccat tgattgactt tcatagaacc
    34381 gttgctgttg aggtagtacc atgaaccatt aacttgtacc caacctgttg ccatggaacc
    34441 atcagtatta taaaaatacc acataccatt ttcttgtttc cagcctgttg ttggagcaac
    34501 tgctttagct ggttctactg ctacatctgt tccttggtta gatgtaacag atacaggata
    34561 cgaaggaata gatgattgct caggaacaac aactttttca ggttctctcg tccctctcct
    34621 tatacgtctt tttaccatct ctttagtaat ttgacgagaa gtagtttctt caattgttcc
    34681 atcacgttca tctacagtat agattgtagt aagagtaatt taccaatttc tcctacttct
    34741 tctacttctt gacttttatc aagagttggg ccatcgagat attctgtttc gattgaaatt
    34801 tcttggacaa gaacttgggg cttggttcct tttttaacaa ctcttgtttg agagtcttct
    34861 ttttgactta aagtactctc agttacttgt ccactctttc catctacatc ataagttatc
    34921 gttgtaacta ttttcccatt ctttcctaga gtaatctctt gctcctgtcc tgcagaaagg
    34981 tcattgtctg cttcatattt agtagcaaat ggaacaaaaa cttcttcaac cttgctttta
    35041 gctggaactt tgataactgt atccgtggct tcttttctat caacagtaac ctgttcggta
    35101 acatgaccag tctctggatt aacatcgtag gtccttgtcg tagttacata gccatcctct
    35161 ccatcaattg taacaggatt ttcactacgg tcttttgttt catctttttc ataacgaatt
    35221 cgcgtacttg aaattttctt ggttactacc ttaggtttag tcgctacttt tacaataata
    35281 tccccattgt cagcgtcatc atactctatt ccctcttctt tatctctagt atcatctctg
    35341 acatattgaa tcccatcagc agcatgaaca aaacttgtat tcagattcct cctaaaaata
    35401 aagttagccc gattaccgca gaaccaaaaa tctttccgag tttacgtatt gcatagcgct
    35461 tattagtatt agattttgcc attacatcct acttctagta tagcatcttt tct
//
LOCUS       DAVLVF010000015        34826 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVLVF010000015 DAVLVF010000000
VERSION     DAVLVF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34826
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.015"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..83)
                     /locus_tag="VYN66_000673"
     CDS             complement(<1..83)
                     /locus_tag="VYN66_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEV1494735.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEE"
     gene            78..332
                     /locus_tag="VYN66_000674"
     CDS             78..332
                     /locus_tag="VYN66_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1494736.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            578..1912
                     /gene="trmFO"
                     /locus_tag="VYN66_000675"
     CDS             578..1912
                     /gene="trmFO"
                     /locus_tag="VYN66_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV1494737.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            1996..2733
                     /locus_tag="VYN66_000676"
     CDS             1996..2733
                     /locus_tag="VYN66_000676"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV1494738.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2742..3299
                     /gene="frr"
                     /locus_tag="VYN66_000677"
     CDS             2742..3299
                     /gene="frr"
                     /locus_tag="VYN66_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV1494739.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3359..4213
                     /gene="cvfB"
                     /locus_tag="VYN66_000678"
     CDS             3359..4213
                     /gene="cvfB"
                     /locus_tag="VYN66_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV1494740.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4222..4437
                     /locus_tag="VYN66_000679"
     CDS             4222..4437
                     /locus_tag="VYN66_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV1494741.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4523..5491
                     /locus_tag="VYN66_000680"
     CDS             4523..5491
                     /locus_tag="VYN66_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV1494742.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(5469..5648)
                     /locus_tag="VYN66_000681"
     CDS             complement(5469..5648)
                     /locus_tag="VYN66_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494743.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            5684..6184
                     /locus_tag="VYN66_000682"
     CDS             5684..6184
                     /locus_tag="VYN66_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1494744.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVIDFVYAHPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6187..6447
                     /locus_tag="VYN66_000683"
                     /pseudo
     CDS             6187..6447
                     /locus_tag="VYN66_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(6814..7140)
                     /locus_tag="VYN66_000684"
     CDS             complement(6814..7140)
                     /locus_tag="VYN66_000684"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494745.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(7086..7499)
                     /locus_tag="VYN66_000685"
     CDS             complement(7086..7499)
                     /locus_tag="VYN66_000685"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEV1494746.1"
                     /translation="MGAHFLTKQAGGSGVLPSGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(7500..7925)
                     /locus_tag="VYN66_000686"
     CDS             complement(7500..7925)
                     /locus_tag="VYN66_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV1494747.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            8102..8671
                     /locus_tag="VYN66_000687"
     CDS             8102..8671
                     /locus_tag="VYN66_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1494748.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTPETNRAVIELAFEKIGMNKLVALYDKDNSASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            8739..9389
                     /locus_tag="VYN66_000688"
     CDS             8739..9389
                     /locus_tag="VYN66_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEV1494749.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            9373..11613
                     /locus_tag="VYN66_000689"
     CDS             9373..11613
                     /locus_tag="VYN66_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV1494750.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPLSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            11755..11979
                     /locus_tag="VYN66_000690"
     CDS             11755..11979
                     /locus_tag="VYN66_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494751.1"
                     /translation="MIIGLFQSSISAVTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSW
                     SRYDSYSEYPVGSGWNYGRYEIINYYSGGY"
     gene            12012..12599
                     /locus_tag="VYN66_000691"
     CDS             12012..12599
                     /locus_tag="VYN66_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494752.1"
                     /translation="MLQLTHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            12603..13784
                     /locus_tag="VYN66_000692"
     CDS             12603..13784
                     /locus_tag="VYN66_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1494753.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIVLALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKMQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQKIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFK
                     V"
     misc_feature    13944..14075
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14091..14621
                     /gene="infC"
                     /locus_tag="VYN66_000693"
     CDS             14091..14621
                     /gene="infC"
                     /locus_tag="VYN66_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV1494754.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14654..14854
                     /gene="rpmI"
                     /locus_tag="VYN66_000694"
     CDS             14654..14854
                     /gene="rpmI"
                     /locus_tag="VYN66_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV1494755.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            14906..15265
                     /gene="rplT"
                     /locus_tag="VYN66_000695"
     CDS             14906..15265
                     /gene="rplT"
                     /locus_tag="VYN66_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV1494756.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15323..15703
                     /locus_tag="VYN66_000696"
     CDS             15323..15703
                     /locus_tag="VYN66_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1494757.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            15953..16753
                     /locus_tag="VYN66_000697"
     CDS             15953..16753
                     /locus_tag="VYN66_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV1494758.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            16764..17702
                     /locus_tag="VYN66_000698"
     CDS             16764..17702
                     /locus_tag="VYN66_000698"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV1494759.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKVTNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            17744..19645
                     /locus_tag="VYN66_000699"
     CDS             17744..19645
                     /locus_tag="VYN66_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEV1494760.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     AKNETVDGYQLGSDGKWLGGKTTNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWL
                     DKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIP
                     VASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINN
                     SLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYL
                     SAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMK
                     INEKLGGKD"
     gene            complement(19706..21361)
                     /gene="pavA"
                     /locus_tag="VYN66_000700"
     CDS             complement(19706..21361)
                     /gene="pavA"
                     /locus_tag="VYN66_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV1494761.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIGLDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21466..21963
                     /gene="ybeY"
                     /locus_tag="VYN66_000701"
     CDS             21466..21963
                     /gene="ybeY"
                     /locus_tag="VYN66_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV1494762.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAKKLGKEDKEMAVTFVT
                     NERSHKLNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            21944..22339
                     /locus_tag="VYN66_000702"
     CDS             21944..22339
                     /locus_tag="VYN66_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV1494763.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22356..23255
                     /gene="era"
                     /locus_tag="VYN66_000703"
     CDS             22356..23255
                     /gene="era"
                     /locus_tag="VYN66_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV1494764.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23304..24128
                     /gene="mutM"
                     /locus_tag="VYN66_000704"
     CDS             23304..24128
                     /gene="mutM"
                     /locus_tag="VYN66_000704"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV1494765.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24128..24733
                     /locus_tag="VYN66_000705"
     CDS             24128..24733
                     /locus_tag="VYN66_000705"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV1494766.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            24720..25919
                     /locus_tag="VYN66_000706"
     CDS             24720..25919
                     /locus_tag="VYN66_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HEV1494767.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            25919..26068
                     /gene="rpmG"
                     /locus_tag="VYN66_000707"
     CDS             25919..26068
                     /gene="rpmG"
                     /locus_tag="VYN66_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1494768.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26108..26341
                     /gene="secG"
                     /locus_tag="VYN66_000708"
     CDS             26108..26341
                     /gene="secG"
                     /locus_tag="VYN66_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV1494769.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26443..28797
                     /gene="rnr"
                     /locus_tag="VYN66_000709"
     CDS             26443..28797
                     /gene="rnr"
                     /locus_tag="VYN66_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV1494770.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNH
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYVDVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGCSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            28760..29227
                     /gene="smpB"
                     /locus_tag="VYN66_000710"
     CDS             28760..29227
                     /gene="smpB"
                     /locus_tag="VYN66_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV1494771.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29243..30103
                     /gene="tehB"
                     /locus_tag="VYN66_000711"
     CDS             29243..30103
                     /gene="tehB"
                     /locus_tag="VYN66_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV1494772.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30176..31129
                     /gene="coiA"
                     /locus_tag="VYN66_000712"
     CDS             30176..31129
                     /gene="coiA"
                     /locus_tag="VYN66_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV1494773.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31148..32950
                     /gene="pepF"
                     /locus_tag="VYN66_000713"
     CDS             31148..32950
                     /gene="pepF"
                     /locus_tag="VYN66_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1494774.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            32952..33665
                     /locus_tag="VYN66_000714"
     CDS             32952..33665
                     /locus_tag="VYN66_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV1494775.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            33732..34673
                     /gene="prsA"
                     /locus_tag="VYN66_000715"
     CDS             33732..34673
                     /gene="prsA"
                     /locus_tag="VYN66_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV1494776.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
BASE COUNT        10481 a         6477 c         7864 g        10004 t
ORIGIN      
        1 atctcttcaa aagtcgtgcg ctgaacacca acaagacgct taaatcgtgc atcagttagt
       61 tgtttacttg cttcatcatt catagaacta ctataccata ttttgtttcg caggaagtct
      121 attggaaagt aagaaatatt gaagctgagg ctattagaag aaattgtgag cgtggtgcta
      181 ttttttcagg taaaataaaa tatcacgaag attcacagtt taaaggagat cactatgttg
      241 aatgttatgc tgttttagat aatacggtta tagcaagaga tagaataaca gtccctatcg
      301 atccgttatg tggaaaagat tttatagagt agcatataat tgattcttaa ctggaatact
      361 ctttatctct ttacatcaag aaaatgacta aacagggaag tttgccttct tccctttttt
      421 tgttatacta gtagaagaaa aaaatagaaa gatttgtggg agtgaaaaag tcctgtggac
      481 tttttcagcc tgagccaaga aactcgaaag ctcgtaagtc tgattggctt tttcaatgtg
      541 aatcttaact tcatactccc aaagaggtat tagtgtcgtg tctcaatctt atatcaatgt
      601 tatcggtgct ggtttggcag gttctgaagc agcctaccaa atcgcagagc gtggtattcc
      661 agttaaactt tatgaaatgc gtggtgtcaa gtcaacacct cagcacaaaa cagacaattt
      721 tgctgagttg gtttgttcca attccttgcg tggagatgct ttgacaaatg ctgttggcct
      781 ccttaaggaa gaaatgcgtc gcttgggttc tgtgattttg gaatctgctg aggctacacg
      841 tgttcctgca ggtggtgccc ttgcagtgga ccgtgatggt ttctctcaaa tggtgaccga
      901 aaaagttgcc aaccacccct tgattgaagt ggttcgtgat gaaattacag aattgccgac
      961 agatgttatt acggttatcg ctactggtcc tttgacaagt gatgccttgg ctgaaaagat
     1021 tcatgccctt aatgacggtg atggttttta tttctacgat gcggcagcgc ctattatcga
     1081 tgtcaacact atcgatatga gcaaggtcta cctcaaatca cgttatgata agggagaagc
     1141 ggcctacctc aatgccccta tgaccaagca agaatttatg gatttccatg aagctttggt
     1201 caatgcagaa gaagcaccgc ttaattcttt tgaaaaagaa aagtactttg aaggatgtat
     1261 gcctatcgaa gtcatggcca aacgtggcat taaaaccatg ctttatggtc ctatgaaacc
     1321 agttggtctt gagtatccgg acgactacac aggacctcgt gatggagaat ttaaaacacc
     1381 ttatgcggtt gtccagctcc gtcaggataa tgcagctggt agcctataca atattgttgg
     1441 tttccagacc cacctcaaat ggggagaaca aaagcgtgtc ttccaaatga ttccgggtct
     1501 tgaaaatgcg gagtttgtcc gttatggtgt catgcatcgc aattcttaca tggattcacc
     1561 aaatcttctt gagcagactt accgttccaa gaaacaacca aatctcttct ttgctggtca
     1621 gatgacgggg gtggaaggct atgttgagtc ggcggcttcg ggcttagttg cgggaattaa
     1681 cgcagctcgt ctcttcaagg aagaaagcga ggttattttc cccgagacga cagcgattgg
     1741 aagcttagct cattacatta cccatgccga cagcaaacat ttccaaccaa tgaatgtcaa
     1801 ttttgggatc atcaaggagt tggaaggcga gcgtatccgt gataagaagg ctcgttatga
     1861 aaaaattgca gagcgtgccc ttgccgactt agaggaattt ttgactgtct aatttttttg
     1921 aaagaattgc tcatgatact ataaaaatct tagaaattgt gataaaatag gtaggatgaa
     1981 agaaggagag tgaaaatggc gaatcccaag tataaacgta ttttaatcaa gttatcaggt
     2041 gaagcccttg ccggtgaacg tggcgtaggg attgatatcc aaacagttca aacaatcgca
     2101 aaagagattc aagaagttca tagcttaggt atcgaaattg cccttgttat tggtggagga
     2161 aatctttggc gtggagaacc tgcagcagaa gcaggtatgg accgtgttca ggcagattac
     2221 actggaatgc ttgggactgt tatgaatgct cttgtgatgg cagattcatt gcaacaagtt
     2281 ggggttgata cgcgtgtaca aacagctatt gctatgcaac aagtggcaga gccttatgtc
     2341 cgtggacgtg cccttcgtca ccttgaaaaa ggccgtatcg ttatctttgg tgctggaatt
     2401 ggttcaccat acttctcgac agatacaaca gcggcccttc gtgcagctga aatcgaagca
     2461 gatgccatcc tcatggctaa aaatggcgtc gatggtgtgt acaatgccga tcctaagaag
     2521 gacaagacag ccgttaagtt tgaagaattg acccaccgtg atgttatcaa caaaggtctt
     2581 cgtatcatgg actcaacagc ctcaaccctc tcaatggaca acgacattga cttggttgtc
     2641 ttcaacatga accaatcagg caacatcaaa cgtgtcgtat ttggtgaaaa tatcggaaca
     2701 acagtttcaa ataatatcga agaaaaggaa taagaaagaa tatggctaac gtaattattg
     2761 aaaaagctaa agagagaatg acccaatctc accaatcact tgctcgtgaa tttggtggta
     2821 tccgtgctgg tcgtgccaat gcaagcttgc ttgaccgtgt acatgtagaa tactatggag
     2881 tcgaaactcc tcttaaccaa atcgcttcaa ttacgattcc agaagcgcgt gttttgttgg
     2941 taacaccatt tgacaagtct tcattgaaag acatcgaacg tgccttgaac gcttctgatc
     3001 ttggtatcac accggctaat gacggttctg tgattcgctt ggttatccca gctcttacag
     3061 aagaaactcg tcgtgacctt gctaaagaag tgaagaaggt cggcgaaaat gctaaagtgg
     3121 ctgtccgcaa tatccgtcgc gatgctatgg acgaagctaa gaaacaagaa aaagcaaaag
     3181 aaatcactga agacgaattg aagactcttg aaaaagatat tcaaaaagta acagacgatg
     3241 ctgttaaaca catcgacgac atgactgcta acaaagagaa agaacttttg gaagtctaaa
     3301 aataaacaga aaaactcagt tggcattgct ggctgagttt tattcgaaag aaggaaatat
     3361 gaatacaaat cttgcaagtt ttatcgttgg actgatcatc gatgaaaatg accgttttta
     3421 ctttgtgcaa aaggatggtc aaacctatgc tcttgctaag gaagaaggtc aacatacagt
     3481 aggggatacg gtcaaaggtt ttgcatacac ggatatgaag caaaaactcc gcctgacaac
     3541 cttagaagtg actgccactc aggaccaatt tggttgggga cgtgtcacag aggttcgtaa
     3601 ggatttgggt gtctttgtgg atacaggcct tcctgacaag gaaatcgttg tgtcactcga
     3661 tattctccct gagctcaagg aactctggcc taagaagggc gaccaactct acatccgtct
     3721 tgaagtggat aagaaagacc gtatctgggg cctcttggct tatcaagaag acttccaacg
     3781 tcttgctcgt cctgcctaca acaacatgca gaaccaaaac tggccagcca ttgtttaccg
     3841 tctcaagctg tcaggaactt ttgtttacct accagaaaat aatatgcttg gttttattca
     3901 tcctagcgag cgttacgcag agccacgttt ggggcaagta ttagatgcgc gcgttattgg
     3961 tttccgtgaa gtggaccgca ctctgaacct ctccctcaaa ccacgctcct ttgaaatgtt
     4021 ggaaaacgat gctcagatga ttttgactta tttggaaagt aatggcggtt tcatgacctt
     4081 aaatgataag tcatctccag acgacatcaa ggcaaccttt ggcatttcta aaggtcagtt
     4141 caagaaagct ttaggtggtc ttatgaaggc tggtaaaatc aagcaggacc agtttgggac
     4201 agagttgatt tagggaggct tatgagaaaa tcattttaca cttggctcat gaccgagcgc
     4261 aatcctaaaa gtaacagtcc caaagcaatt ttggcagacc tcgcttttga agaggcagct
     4321 tttccaaaac atacagatga ttttgatgag gtcagtcgct ttttggagga gcatgccagt
     4381 ttctctttta acctaggaga ttttgacagc atttggcagg aatatctaga acactagcat
     4441 ttattcattg ggtttgggct agtaatttct ccatccctct gctataataa aaagaaataa
     4501 aaggattaga gaggttcttt atttgaagga acattcaata gacattcaac tgagtcatcc
     4561 agatgacctg tttcatcttt ttggttccaa tgaacgccat cttcgtttga tggaagaaga
     4621 gcttgatgtt gtgattcatg ctcgtacgga gattgtccag gttttgggag aagagtctgc
     4681 ctgtgaggaa gcccgtcaag ttattcaggc tttgatggtc ttggtaaatc gtgggatgac
     4741 cgttggtacg ccagatgtag tcactgcgat tagcatggtc aaaaatgatg aaattgacaa
     4801 gtttgtcgcc ctttacgaag aagaaattat caaagataat actgggaaac ctatccgtgt
     4861 caaaacccta gggcaaaagc tttatgtgga cagtgtcaaa cagcatgatg tgacctttgg
     4921 aattgggcca gcaggtacag ggaagacctt ccttgcagtg accttggcag tgactgccct
     4981 taaacgtggg caagtcaagc gaattatcct aactcgtcca gcggtggaag cgggagagag
     5041 tcttggattt cttccgggtg atcttaagga gaaggtggat ccttaccttc gtcctgttta
     5101 cgatgccttg tatcagattc tggggaaaga ccaaacaact cgactcatgg agcgtgaaat
     5161 tatcgagatt gcgccccttg cctatatgcg tggacggacc ttggatgatg cctttgtcat
     5221 tctcgatgag gcgcaaaata cgaccatcat gcagatgaag atgttcttga ctcgtttagg
     5281 ttttcattct aagatgattg tcaatggaga tattagtcag attgacctgc cacgtaatgt
     5341 caagtccggt ttgattgatg ctcaagagaa actcaagaac atccatcaga ttgactttgt
     5401 tcatttttca gccaaggatg tggttcgcca tccagttgtc gctcagatta tccgagccta
     5461 tgaatattct actgaagttg cacacgactg attttgagga agtttgcctg caaaagaata
     5521 tacttttgag agaggaaaat ccagttgtat aggctaaagg ttttatccaa aggtttgaga
     5581 caacgattag gcacgatgga aagaactttt atgtggctga tgacgatcag tgcatcttcc
     5641 tgtgtcataa tcacagtgca caagaaagta ggaatttgaa aagatgattg accaactatc
     5701 taagtattac agttgtagga tactaactga aaaggatatt ccaagtattt tatctttata
     5761 tgaaagtaat cctctgtatt ttcagcattg tccaccagag ccaaattttg caactgtaaa
     5821 agaggacatg ctttgtctac ctgaaggtaa agctaaggct gataagtttt ttgttggatt
     5881 ttggaatgga tctgaccttg tggctgttat agattttgtc tatgcacatc ctgatgagga
     5941 gactgttttt attggtttgt ttatggttga tcaagcctat cagaggaaag ggattggtag
     6001 tcatattgtg acagaagcac tagcttattt tgctaagaac tttcgaaagg cacgtttggc
     6061 ttatgttaag ggaaatccgc aatctcagca tttttgggaa aagcagggct ttaaatcaat
     6121 tggatgcgag gttaagcaag aactctatac ggttgttatc gctgaacaga gcctagaaga
     6181 ttagaaatgg catcaagtaa gaactatttg gaatttgttt tggaacaatt atcaggatta
     6241 gatgatgtga cttaccgttc catgatgggg gagtatattc tttacttccg cggcaagatt
     6301 attggcggca tttatgacga tcgcttttta gttaaacccg tgcaagcagt cttagataag
     6361 attgaccaat cttcttttga gtttccatac aaaggtgcca aagaaatgat ttgagtggaa
     6421 gaacttgata ataagatgtt tctataagac ctgattttag ctatgtataa ccaactgcca
     6481 acgcccaaac ctaaaaagaa aaagcaaggg tgaacgaagt aaaaaagaag tctgctaagg
     6541 ccctgtcttt gcacgggtaa aattttatat ataaaaagaa gctgggacta aagagctcag
     6601 cttcctttgg tttatataat tgtcattaca agacgaagtg gttgggcgaa actctgttga
     6661 ctttattcaa tttagagttt cttatgcaca attgagtctg gaacgaaagt ctccagttgc
     6721 aaagtataca gtacaataaa ccaacgatgt aatagctgat gacacaaagc acagtgggta
     6781 ggacttgcga agtcaccctt ttcttttcaa aatttatact aaatcattga tatcagtgta
     6841 gtcacgatta agtccttgag caactggtag gctagtcaag taaccttgat aagtggtcac
     6901 accttgacgc aagccttcat cttcagagat tgcttgtgcg aatcctttgc cagccaaagc
     6961 ttcgatataa ggaagagtga cattggttag ggcgatggtt gaagtgcggg caaccgcacc
     7021 agggatattg gcaacggcat agtggagaac accgtgtttt tcatagacgg gttcatcgtg
     7081 cgttgtcaca cggtcagctg tttcgataac gccaccttgg tcaacagcaa cgtcaacgat
     7141 acagagcctg gacgcatttg tttgaccatc tcatctgtca ccaattccgg tgcttttgca
     7201 ccagggatga gaatggctcc aatcaccaca tcagcatctc tcatacttgc ttcaatgttg
     7261 aatgaattag atataagagt ttgaatttga cttccaaaga cttcttctag aactgagaga
     7321 cgcttggcac taatatctaa aatagtcact tgagcaccaa gaccaagggc gatgcgggca
     7381 gcatgtgtac cgacgacacc atcaccgatg atagttactt ttccttttgg aacacctggt
     7441 acaccactag gtagaacacc agagccacca gcttgcttag taaggaagtg agctccgatt
     7501 taaacagcca tacgacctgc aacctcactc ataggaacga ggagcggtag ttgtccttga
     7561 ttgtcacgaa cagtttcata ggcaattcct gttgtttttg ctgctaacat agcatctgct
     7621 aattctggag cagcggccat gtgcaagtag gtgaagagaa gaagatcgtc gcgcaagtaa
     7681 ccgtattcag aacttaaagg ttcttttact ttcacaacca actctgctgc ccaagcttca
     7741 ccagcagtag cgacaatctc agctccttgc ttttgatagt cagcatcagt aaagccagaa
     7801 ccgagaccag catttgtttc gataaggaca cgatgaccac gactaactaa gctatgaaca
     7861 cctgcaggtg tgagggcgac acggttttcg ttatttttaa tttcttttgg gattccgatt
     7921 aacattgaga taacctacct ttcaattgac ggtcttgttt tggttgtcac attccagttc
     7981 ataaatcaaa aatgtgacgg tttcattgta tatgaaaccg cttcaaaaat caagaaaaac
     8041 ttgtcatcca aattttttta tgctagacta gtgaaaatca agctctaatg gagggaaaag
     8101 tatggaatca atatttgtga aatttgccca gtatccgtct atagaaacgg agcgtttatt
     8161 gcttagacct gtaactttgg atgatgcgga agcaatgttt gactatgcct cggacaaggg
     8221 taatacacgt tacacttttc caaccaatca aagcttggaa gaaaccaaga ataacattgc
     8281 tcagttctac ttggctaatc ccttgggacg ttgggggatt gaattaaaag ataacggcaa
     8341 gtttatcgga actattgact tacacaagat tgatcctgtt cttaagaagg cagctattgg
     8401 ctacattatc aataaaaagt attggaatca aggattaacg ccagaaacca atcgtgctgt
     8461 gattgagcta gcttttgaga agataggaat gaataagtta gttgctttat acgataagga
     8521 caattctgcg tcaggaaagg tcatggagaa atcaggcatg cgtttttccc atgcagaacc
     8581 atatgcttgt atggaccagc atgaaaaagg ccgaatcgtg acaagagttc attatgtctt
     8641 gaccaaggaa gactattttg caaataaata agcagttgaa aagaaatttt tcgactgttt
     8701 tttcttcctc ttacgaataa tctaagagag gagaaaatat ggaagcaatt atcgagaaaa
     8761 tcaaagagta taaaatcatc gtcatctgta ctggtctggg cttgcttgta ggcggatttt
     8821 tcctgctaaa gccagctcca caaacacctg taaaggaaac gaatttgcag gctgaagttg
     8881 cagctgtttc caaggatttg gtatccgaaa aggaagtgaa caaggaagag aaggaagaac
     8941 cccttgaaca agatctaatc acagtagatg tcaaaggtgc tgtcaaatcg ccagggattt
     9001 atgacttgcc tgtaggtagt cgagtcaatg atgctgttca gaaggctggt ggcttgacag
     9061 agcaagcaga cagcaagtcg ctcaatctag ctcagaaagt tagtgatgag gctctggttt
     9121 acgttcctac taagggagaa gaagcagtta gtcaacagac tggttcgggg acagcttctt
     9181 caataagcaa ggaaaagaag gtcaatctca acaaggccag tctggaagaa ctcaagcagg
     9241 tcaagggact gggaggaaaa cgagctcagg acattatcga ccatcgtgag gcaaatggca
     9301 agttcaagtc agtagacgag ctcaagaagg tctctggcat tggtggcaaa acaatagaaa
     9361 agcttaaaga ctatgttaca gtggattaag aatttctcta ttcccctaat ttacctgagt
     9421 tttctattac tttggcttta ttacgctatt ttctcagcat cttatcttgc tttgttgggc
     9481 tttgtttttc tgctagtctg tctctttatc caatttccgt ggaaatctgc tggtaaagtt
     9541 ctaataattt gcggaatctt tggattttgg tttgtttttc aaaattggca acagagtcaa
     9601 gcgagtcaaa atctggcgga ttctgttgaa agggtacgga ttttgcctga tactattaag
     9661 gttaatggtg atagtctatc ctttcgtggc aagtctaacg gtcgtgcttt ccaagtctat
     9721 tataaactcc agtccgagga ggagaaagaa gcctttcaag ctttaaccga cctgcatgag
     9781 ataggactag aagggaagct ttcggagcca gaagggcaga gaaattttgg tggctttaat
     9841 taccaagcct atctgaagac tcagggaatt taccagactc tcaatatcaa aacaatccag
     9901 tcacttcaaa agattggcag ttgggatata ggagaaaact tgtccagttt acgtcgaaag
     9961 gctgtggttt ggattaagac gcactttcca gaccctatgc gcaattacat gacaggactc
    10021 ttgctgggac atctggacac cgactttgag gagatgaatg agctttattc cagtctagga
    10081 attatccacc tctttgccct atctggtatg caggtaggtt ttttcatgaa tggatttaag
    10141 aaacttctct tgcgattggg cttgacccaa gaaaagttga aatggctgac ttatcccttt
    10201 tcccttatct atgcgggact aactggattt tcagcatcgg ttattcgcag tctcttgcaa
    10261 aagctactgg ctcaacatgg ggttaagggc ttggataatt ttgccttgac ggtgcttgtc
    10321 ctctttattg tcatgccaaa ctttttcttg acagcaggag gagtcttgtc ctgcgcttat
    10381 gcttttatcc tgaccatgac cagcaaagaa ggggaggggc tcaaggctgt tactagtgaa
    10441 agtctagtca tctccttggg catattgccc attctatcct tctattttgc ggaatttcaa
    10501 cctttgtcta tccttttgac ctttgtcttt tcctttctat ttgacttgac cttcttaccg
    10561 ctcttgtcta ttttatttgt cctttccttt ctctatccag tcattcagct gaactttatc
    10621 tttgaatggt tggagggcat tattcgcttg gtgtcacagg tgacaagtag acctctggtc
    10681 tttggacaac ccaatgcatg gtttttaatc ctattgttaa tttccttggc tttggtctat
    10741 gatttgagaa aaaacattaa aaagctaacg gtattgtgct tattgattac agggctcttt
    10801 ctcctgacca agcatccact ggaaaatgaa atcaccatgc tggatgtggg gcaaggagaa
    10861 agtattttcc tacgggatgt aactgggaaa accattctca tagatgtagg tggtaaggca
    10921 gaatcttata agaaaatcga aaaatggcaa gaaaaaatga cgaccagcaa tgcccagcga
    10981 accttgattc cctatctcaa aagtcgagga gtagctaaga ttgaccagct aattttgact
    11041 aacacggaca aggagcatgt tggagatttg tcagaggtga ccaaggcttt ccatgtaggg
    11101 gagattctag tatcaaaaga cagtctgaaa cagaaggaat ttgtggcaga actacaggcg
    11161 actcaaacaa aggtgcgtag tatgacagta ggggagaact tgcccatttt tggaagtcag
    11221 ttagaagttc tatctccaag gaaaatggga gatggaggac acgatgatac cctagttctg
    11281 tatgggaaat tcttggataa gcaatttctc ttcacgggaa atttggagga gaaaggagag
    11341 aaggacttgc tgaagcacta tccagacttg aaagtaaatg ttttgaaagc tagccaacat
    11401 ggcaataaaa aatcatcaag tccagccttt ctagaaaaac tcaaaccaga gcttactctt
    11461 atctcagttg gaaagagcaa tcgaatgaaa ctcccccatc aggaaacatt gacacgactg
    11521 gaaggtatca atagcaaagt ttatcgaact gaccagcaag gagctatacg ttttaagggg
    11581 ttggatagtt ggaaaatcga aagtgttcga taggaaggat aaatgttgta gattagtgaa
    11641 ataaactaaa aatttgttgc ataataatga taaaaatggt ataatgaaaa cgtattcaat
    11701 attgaggata taaaatcatt aaaaattagc aaaaatcgtt taattagtta gtttatgatt
    11761 attggtcttt ttcagtccag tatatctgct gtgacagtca ctaaaagtta caagtatgat
    11821 tggaatacgg tttgggaata tagtaccaac tatcacgacc atcagtatgc ttggattccg
    11881 tcatggtctc gttatgacag ctattctgag tatccagttg gttctggctg gaactacggt
    11941 cgttatgaga tcataaacta ttacagcgga ggctattaat tcttaaagag tgagaaaaag
    12001 gagggctaga tatgttgcag cttactcatg tgaccttaaa aacgcgacaa gtcatcttgc
    12061 aagatgtgga tttcaccttt aaaaagggta gggtttatgg tcttcttgct atcaatggct
    12121 ctggaaagac gaccctgttc cgtgccatta gcaatttaat tcccataagt agtggaaata
    12181 tcgcagcccc tccttcttta ttttattatg agagtattga atggctggat ggaaacttaa
    12241 gtgggatgga ctaccttcgt cttatcaaaa acatctggaa gtcaggtctg aacttgaggg
    12301 atgaaatcgc ctattgggaa atgtctgact atatcagtct tcccattcgc aagtattcct
    12361 taggcatgaa gcaacgcttg gtgattgcca tgtatttcct cagtcaggcc aaatgctggc
    12421 tcatggatga gattacaaat ggcttagatg agtattatcg acagaagttt tttgataggc
    12481 tagcacaaat cgatagacaa gaacagctgg ttcttttaag ttcccactat aaggaagagt
    12541 tggttgatgt ctgcgataga gtagtaacca ttcatcaggg gcagatagaa gaggtttagt
    12601 ttatgaaaga tgttagtcta tttttattga aaaaagtttt caaaagccgc ttaaactgga
    12661 ttgtcttagc tttatttgta tctgtactcg gtgttacctt ttatttaaat agtcagactg
    12721 caaactcaca cagcttggag agcaggttgg aaagtcgcat tgcagccaac gagagggcta
    12781 tcaatgaaaa tgaagagaaa ctctcccaaa tgtctgatac cagctcggag gaataccagt
    12841 ttgctaaaaa taatttagac gtgcaaaaaa atcttttgac gcgaaagaca gaaattctaa
    12901 ctttactaaa agaagggcgc tggaaagaag cctactattt gcagtggcaa gatgaagaga
    12961 agaattatga atttgtatca aatgacccga ctgctagctc tggcttaaaa atgggggttg
    13021 accgcgaacg gaagatttac caagccctgt atcccttgaa cataaaagca catactttag
    13081 agtttccgac ccacgggatt gatcagattg tctggatttt agaggttatc atcccaagct
    13141 tgtttgtggt tgcgattatt tttatgctaa cacaactatt tgcagaaaga tatcaaaatc
    13201 atctggacac agctcactta tatcctgttt caaaagtgac atttgcaata tcctctcttg
    13261 gagttggagt gggatatgta actgtgctgt ttatcggaat ctgtggcttt tcttttctag
    13321 tgggaagtct gataagtggt tttggacagt tagattatcc ctacccgatt tatagcttag
    13381 tgaatcaaga ggtaactatt ggaaagatgc aggatgtatt atttcctggc ttgctcttag
    13441 ctttcttagc ctttatcgtc attgtggaag ttgtgtattt gattgcttac tttttcaagc
    13501 aaaaaatgcc tgtcctcttt ctttcactca ttgggattgt tggcttattg tttggcatcc
    13561 aaaccattca gcctcttcaa aagattgcac atctgattcc ctttacttac ttgcgttcag
    13621 tggagatttt atctggaaga ttacctaagc agattgataa tgtcgatcta aattggagca
    13681 tggggatggt cttacttcct tgcctgatta tccttttgtt agtggggatt ctatttatcg
    13741 aaagatgggg aagttcacgt aaaaaggaag tttttaaagt ttagttttcc tatagggaag
    13801 ctaaagaaaa actaacatag ggagagaatc aacttggttc tctctttttg attcgaaaac
    13861 caaaccaaaa tacaaacaca aacttttcaa aaaataactt tttatcttga caagagctag
    13921 aaaacttggt atcatataaa agttgagaaa agcagaagtg agagcttctc gccttgtgac
    13981 attaagttgc ctggccctac ggatgaaaag tttcgaagaa acgctatcat aacgtgcggg
    14041 cttgtatatt tacgagtccg ctattgtttt tctctaataa aacaaaagag gtgaaaacca
    14101 tagcaaagca agacttattc atcaatgatg agattcgtgt acgtgaagtt cgcttgattg
    14161 gtcttgaggg agaacagcta ggcatcaagc cactcagtga agcgcaagct ttggctgata
    14221 acgctaatgt tgacctagta ttgattcaac cccaagccaa accgcctgtt gcaaaaatta
    14281 tggactacgg taagttcaaa tttgagtacc agaagaagca aaaagaacaa cgtaaaaaac
    14341 aaagcgttgt tactgtgaaa gaagttcgtc taagtccaac aattgacaag ggtgactttg
    14401 atacaaaact tcgtaatgca cgtaagttcc ttgaaaaagg aaataaagtt aaggtatcta
    14461 ttcgctttaa gggtcgtatg attacccata aagagattgg tgcaaaagtt ttagccgagt
    14521 ttgctgaagc aactcaagat attgccatca tcgaacaacg tgctaaaatg gatggacgtc
    14581 aaatgttcat gcaattggcg ccagcgactg acaaaaaata attgtcagaa agttaaataa
    14641 aggagaaaaa atcatgccaa aacaaaaaac acaccgcgca tcagctaaac gtttcaaacg
    14701 tacaggttct ggtggactta aacgtttccg tgcttacact tctcaccgtt tccacggaaa
    14761 aactaagaaa caacgtcgtc atcttcgtaa agcatctatg gtgcattcag gagattacaa
    14821 acgtatcaaa gcaatgctta ctcgcttgaa ataatagctt gactgtaagt aaacaattct
    14881 agaaaatatt tggaggaaat aaaacatggc acgtgttaaa ggtggcgttg tatcacgcaa
    14941 acgtcgtaaa cgtattctta aattagcaaa aggttactat ggagctaaac acatcttgtt
    15001 ccgtactgca aaagaacaag taatgaactc ttactactat gcataccgtg accgtcgtca
    15061 gaaaaaacgt gacttccgca aattgtggat tactcgtatc aatgcggcgg ctcgtatgaa
    15121 cggactttca tactcacaat tgatgcatgg tttgaaattg gctgagatcg aagttaaccg
    15181 taaaatgctt gctgacttgg ctgttaacga tgcagtagct ttcacagctc ttgcagatgc
    15241 agctaaagca aaacttggta aataacaact gatgagactg gaacaggatt gtccagtctt
    15301 tttaaaaata aaggagaaag atatggcttc aaaaatgcta cacacttgct tacgagtaga
    15361 aaatcttgaa aaatcaatcg cattttatca agatgctttt ggttttaaag aattgcgtcg
    15421 cagagatttt ccagaccatg ccttcacgat tgtctatcta ggtcttgagg gtgatgatta
    15481 tgagttggag ttgacttata actacgatca cggtccgtat gtggttggtg atgggtttgc
    15541 ccatatcgcc ctcagtacac ctgatcttga ggcacttcat caagaacaca gtgcaaaagg
    15601 ctatgaagtg actgagccaa atggtctacc aggaactaca ccaaactatt actttgttaa
    15661 agaccctgat ggatataagg tcgaagtcat tcgtgaaaaa taatttcgtg caagttaagt
    15721 agaatattcg ttttctactt gacttttttt agctgaaaga gtatactaat aaaaattcaa
    15781 cctttaaggg gagtccagag agactcacaa ggtgtcagat aaaagaatag tgcaagtttc
    15841 tagaggagac cttttgagtg tgctctcttg tgttgtacga ttttaactga ggccttgcac
    15901 tagcaaggtc ttttctttat ctggtcccct taaaatttaa ggaggaaaag ttatgaatct
    15961 cacatgtaag aagcgtttgg gtgtcattcg gttggaaacc atgaaggtgg ttgcacaaga
    16021 ggaaatcgcg ccagcaatct ttgaattagt cctagaagga gaaatggttg aagccatgcg
    16081 agcaggccaa tttcttcatc tgcgtgtacc agacgatgcc catctcttac gtcgtcctat
    16141 ttcaatttcg tctattgaca aggcaaacaa gcagtgtcac ctcatttatc ggattgaggg
    16201 agctgggact gcaatttttt caaccttaag tcagggagac actcttgatg tgatggggcc
    16261 tcagggaaat ggttttgact tgtctgacct tgatgagcag aatcaggttc tccttgttgg
    16321 tggtgggatt ggtgttccac ccttgcttga ggtggccaag gaattgcatg aacgtggagt
    16381 gaaagtagtg acagtcctcg gttttgctaa taaggatgct gttattttga aaacggaatt
    16441 ggctcagtat ggtcaggtct ttgtaacgac agatgatggt tcttatggca tcaagggaaa
    16501 tgtttccgtt gttatcaatg atttagacag tcagtttgat gctgtttact cgtgtggggc
    16561 tccaggaatg atgaagtata tcaatcaaac ctttgatgat cacccaagag cctatttatc
    16621 tctggaatct cgtatggctt gtgggatggg agcttgctat gcctgtgttc taaaagtacc
    16681 agaaagcgag acggtcagcc aacgcgtctg tgaagatggt cctgttttcc gcacaggaac
    16741 agttgtatta taaggagaaa attatgacta caaatcgatt acaagtttct ctacctggtt
    16801 tggatttgaa aaatccgatt attccagcat caggctgttt tggctttgga caagagtatg
    16861 ccaagtacta tgatttagac cttttaggtt ctattatgat caaggcgaca acccttgaac
    16921 cacgttttgg aaatccaact ccaagagtgg cagagacgcc tgctggtatg ctcaatgcaa
    16981 ttggcttgca aaatcctggt ttagaggttg ttttggctga aaagctacct tggctggaaa
    17041 gagaatatcc aaatcttcct attattgcca atgtagctgg tttttcaaaa caagagtatg
    17101 cagctgtttc tcatgggatt tccaaggtaa ctaatgtaaa agctatcgag ctcaatattt
    17161 cttgtcccaa tgttgaccac tgtaatcatg gacttttgat tggtcaagat ccagatttgg
    17221 cttatgatgt ggtgaaagca gctgtggaag cctcagaagt gccagtttat gtcaaattaa
    17281 ccccgagtgt gaccgatatc gttactgtcg caaaagctgc agaagatgcg ggagcaagtg
    17341 gcttgaccat gatcaatact ctggttggaa tgcgctttga cctcaaaacc agaaaaccaa
    17401 tcttggccaa tggaacaggt ggaatgtcag gtccagcagt ctttccagta gccctcaaac
    17461 tcatccgcca agtagcccaa acaacagacc tgcctatcat tggaatgggg ggagtggatt
    17521 cggctgaagc tgccctagaa atgtatctgg ctggggcatc tgctatcgga gttggaacag
    17581 ctaactttac caatccttat gcctgccctg acatcatcga aaatttacca aaagtcatgg
    17641 ataaatacgg tattagcagt ctggaagaac tccgtcagga agtaaaagag tctctgaggt
    17701 aaactgcaat caatctgttc ttgatttttt attagtttgt aatatgaatt taggagaatt
    17761 ttggtacaat aaaataaata agaacagagg aagaaggtta atgaagaaag taagatttat
    17821 ttttttagct ctgctatttt tcttagctag tccagagggt gcaatggcta gtgatggtac
    17881 ttggcaagga aaacagtatc tgaaagaaga tggcagtcaa gcagcgaatg agtgggtttt
    17941 tgatactcat tatcaatctt ggttctatat aaaagcagat gctaactatg ctgaaaatga
    18001 atggctaaag caaggtgacg actattttta cctcaaatct ggtggctata tggccaaatc
    18061 agaatgggta gaagacaagg gagcctttta ttatcttgac caagatggaa agatgaaaag
    18121 aaatgcttgg gtaggaactt cctatgttgg tgcaacaggt gccaaagtaa tagaagactg
    18181 ggtctatgat tctcaatacg atgcttggtt ttatatcaaa gcagatggac agcacgcaga
    18241 gaaagaatgg ctccaaatta aagggaagga ctattatttc aaatccggtg gttatctact
    18301 gacaagtcag tggattaatc aagcttatgt gaatgctagt ggtgccaaag tacagcaagg
    18361 ttggcttttt gacaaacaat accaatcttg gttttacatc aaagaaaatg gaaactatgc
    18421 tgataaagaa tggattttcg agaatggtca ctattattat ctaaaatccg gtggctacat
    18481 ggcagccaat gaatggattt gggataagga atcttggttt tacctcaagt ttgatgggaa
    18541 aatagctgaa aaagaatggg tctacgattc tcatagtcaa gcttggtact acttcaaatc
    18601 cggtggttac atgacagcca atgaatggat ttgggataag gaatcttggt tttacctcaa
    18661 gtctgatggc aaaatggctg aaaaagaatg ggtctacgat tctcatagtc aagcctggta
    18721 ctacttcaaa tctggtggct acatggcgaa aaatgagaca gtagatggtt atcagcttgg
    18781 aagcgatggt aaatggcttg gaggaaaaac tacaaatgaa aatgctgctt actatcaagt
    18841 agtgcctgtt acagccaatg tttatgattc agatggtgaa aagctttcct atatatcgca
    18901 aggtagtgtc gtatggctag ataaggatag aaaaagtgat gacaagcgct tggctattac
    18961 tatttctggt ttgtcaggct atatgaaaac agaagattta caagcgctag atgctagtaa
    19021 ggactttatc ccttattatg agagtgatgg ccaccgtttt tatcactatg tggctcagaa
    19081 tgctagtatc ccagtagctt ctcatctttc tgatatggaa gtaggcaaga aatattattc
    19141 ggcagatggc ctgcattttg atggttttaa gcttgagaat cccttccttt tcaaagattt
    19201 aacagaggct acaaactaca gtgctgaaga attggataag gtatttagtt tgctaaacat
    19261 taacaatagc cttttggaga acaagggcgc tacttttaag gaagccgaag aacattacca
    19321 tatcaatgcc ctttatctcc ttgcccatag tgccctagaa agtaactggg gaagaagtaa
    19381 aattgccaaa gataagaata atttctttgg cattacagcc tatgatacga ccccttacct
    19441 ttctgctaag acatttgatg atgtggataa gggaatttta ggtgcaacca agtggattaa
    19501 ggaaaattat atcgataggg gaagaacttt ccttggaaac aaggcttctg gtatgaatgt
    19561 ggaatatgct tcagaccctt attggggcga aaaaattgct agtgtgatga tgaaaatcaa
    19621 tgagaagcta ggtggcaaag attagtacta taagtgaata tgatttgagt gaatagtaag
    19681 ttaaaaatcc tgatttcaag taaaatcagg attttttcat ggatgcaatt tttttggagt
    19741 ctggtgtgac gcggagggtc ttttgtcctg tgtaagtgac aaagccgggt tttccaccag
    19801 ttggtttatt gagttttttg acttcaatca tatctacctg caccagattc gacaggcgcc
    19861 cttgagagaa gtaggcagct aactctgctg cgtctgtctt gactgcatca gatgggtcaa
    19921 gatttcctga gatgacaaca tggcttccag gaatgtcctt agcatggaac caaagttcct
    19981 ccttgcgggc cattttaaag gtcaattcct cattttgaag attgtttcgt ccgacataga
    20041 tgatggtttt gccatcgctt gctagatatt gttctagttt tttgcgtttc tggattttct
    20101 cccgttgtct tctgcggata aaacctgttt gaatcaattc ttcacggatt tcagcgattt
    20161 cttccagtcc agcttggttg aggacggttt ctacactttc cagatagaga atagtggctt
    20221 tggtttcttc aatcaaatca gtcaagtatt tgacagcttc tttgagtttc tgataccgtt
    20281 taaaatagcg ttgggcattc tggttgggag tcagagcctt atcaagccca atcatgatag
    20341 gttggttggt atagtagttg tctaggataa cctggtcttg gtcgttaggc acttggtgga
    20401 ggaaggttgt cagcaattct cctttttgac gaaattcttc agcgttgtct gtcgccagta
    20461 attctttttc ctgttttttg agtttgtgtc ggtttttctg aagttcattt tcaacacgac
    20521 gaatcagttc actggcctgc tgtttgacgc ggtcgcgctc agccttatcc ttatagtagg
    20581 tgtccaacaa atcagaaaga tttgcaaaag gctctcccac ctgatttgca aaaggaactg
    20641 gactgaagga agtctcagtc aagcatggct tggtttcctg attgaaaaaa tttcggaaag
    20701 cggaaagttt ttcactaacc agtatccttt ccaattcatt tgccgtatcg cgtcccagac
    20761 cttgaaagag gctttgaaga ttttttgctg ttagttcttg ggtttgcagg atttcaaaga
    20821 gcttttcatc cttgatagta aaaggattga gagattctgt acttggcgga gcgatatagg
    20881 tcgatcctgg aagtaaggtg cggtagctat tttgtgaaaa gccgacgtgt ttgataactt
    20941 cgaggatttt atgactgctt ttatcgacca gtagaatatt actgtgtttc cccataattt
    21001 cgataatcaa ggtagcctgg atatggtctc caatctcgtt tttattggaa actgtcattt
    21061 ccacaatacg gtcattttcc acttgctcaa tcgactcaat cagggccccc tgcaaatact
    21121 ttctcaaaac catgataaag gtagaaggtt gggctggatt ttcaaaagtc gtttgggtca
    21181 gctgaatgcg tccaaaaact ggatgggcag aaaggagcag gcgatggctt tggcgattgc
    21241 tgcggatttg caagaccaac tcttgttcaa aaggctgatt gattttctgg atgcgaccat
    21301 tcactaattc gcttcgcaat tcctcaacta tgtggtgtaa aaaaaatccg tcaaatgaca
    21361 tcgttctctc cttgtgattg tattccatag tattatatca aaaaggtaga ataaaatcat
    21421 ggaaatgtgg tataataaag ccaagtaaag agaaacgaga agcacatgta tattgaaatg
    21481 gtagatgaaa ctggtcaagt ttcaaaagaa atgttgcaac aaacccaaga aattttggaa
    21541 tttgcagcca aaaaattagg aaaagaagac aaggagatgg cagtcacttt tgtgaccaat
    21601 gagcgtagtc ataaacttaa tctggagtac cgtgacaccg accgtccgac agatgtcatc
    21661 agccttgagt ataaaccaga attggaaatt gcctttgacg aagaggattt gcttgaaaat
    21721 ccagaattgg cagagatgat gtctgagttt gatgcctata ttggggaatt gttcatctct
    21781 atcgataagg ctcatgagca ggccgaagaa tatggtcaca gctttgagcg tgagatgggc
    21841 ttcttggcag tacacggctt tttacatatt aacggctatg atcactatac tccggaagaa
    21901 gaagcggaga tgttcggttt acaagaagaa attttgacag cctatggact cacaagacaa
    21961 taaacgaaaa tggaaaaatc gtgacctgat atccagttta gaatttgcca tcacagggat
    22021 ttttactgct atcaaggaag aacgcaatat gcgaaaacat gcagtgacgg ctctagtggt
    22081 catccttgca ggttttggtt ttcaggtgtc acgaatcgaa tggctctttc tcctattgag
    22141 cattttcttg gtggtagcct ttgaaattat caattctgct attgaaaatg tggtggattt
    22201 ggccagtcac tatcactttt ccatgctggc taaaaatgcc aaggatatgg cggctggcgc
    22261 ggtattagtg gtttctcttt ttgcagcctt aacaggcgca ttgatttttc tcccacgaat
    22321 ctgggattta ttattttaaa cagtaagagg aaattatgac ttttaaatca ggctttgtag
    22381 ccattttagg acgtcccaat gttgggaagt caaccttttt aaatcacgtt atggggcaaa
    22441 agattgccat catgagtgac aaggcgcaga caacgcgcaa taaaatcatg ggaatttaca
    22501 cgactgataa ggagcaaatt gtctttatcg acacaccagg gattcacaaa cctaaaacag
    22561 ctctcggaga tttcatggtt gagtctgcct acagtaccct tcgcgaagtg gacactgttc
    22621 ttttcatggt gcctgctgat gaagcgcgtg gtaaggggga cgatatgatt atcgagcgtc
    22681 tcaaggctgc caaggttcct gtgattttgg tggtgaataa aatcgataag gtccatccag
    22741 accagctctt gtctcagatt gatgacttcc gtaatcaaat ggactttaag gaaattgttc
    22801 caatctcagc ccttcaggga aataacgtgt ctcgtctagt ggatattttg agtgaaaatc
    22861 tggatgaagg tttccaatat ttcccgtctg atcaaatcac agaccatcca gaacgtttct
    22921 tggtttcaga aatggttcgc gagaaagtct tgcacttaac tcgtgaagag attccgcatt
    22981 ctgtagcagt agttgttgac tctatgaaac gagacgaaga gacagacaag gttcacatcc
    23041 gtgcaaccat catggtcgag cgcgatagcc aaaaagggat tatcatcggt aaaggtggcg
    23101 ctatgcttaa gaaaatcggt agcatggccc gtcgtgatat cgaactcatg ctaggagaca
    23161 aggtcttcct agaaacctgg gtcaaggtca agaaaaactg gcgcgataaa aagctagatt
    23221 tggctgactt tggctataat gaaagagaat actaagtaga ggtaggctca tgcctgcttc
    23281 ttgtttttac agaaggagga cttatgcctg aattacctga ggttgaaacc gtttgtcgtg
    23341 gcttagaaaa attgattata ggaaagaaga tttcgagtat agaaattcgc taccccaaga
    23401 tgattaagac ggatttggaa gagtttcaaa gggaattgcc tagtcagatt atcgagtcaa
    23461 tgggacgtcg tggaaaatat ttgctttttt atctgacaga caaggtcttg atttcccatt
    23521 tgcggatgga gggcaagtat ttttactatc cagaccaagg acctgaacgc aagcatgccc
    23581 atgttttctt tcattttgaa gatggtggca cgcttgttta tgaggatgtt cgcaagtttg
    23641 gaaccatgga actcttggtg cctgaccttt tagacgccta ctttatttct aaaaaattag
    23701 gtcctgaacc aagcgaacaa gactttgatt tacaggtctt tcaatctgcc cttgccaagt
    23761 ccaaaaagcc tatcaaatcc catctcctag accagacctt ggtagctgga cttggcaata
    23821 tctatgtgga tgaggttctc tggcgagctc aggttcatcc agctagacct tcccagactt
    23881 tgacagcaga agaagcgact gccattcatg accagaccat tgctgttttg ggccaggctg
    23941 ttgaaaaagg tggctccacc attcggactt ataccaatgc ctttggggaa gatggaagca
    24001 tgcaggactt tcatcaggtc tatgataaga ctggtcaaga atgtgtacgc tgtggtacca
    24061 tcattgagaa aattcaacta ggtggacgtg gaacccactt ttgtccaaac tgtcaaagga
    24121 gggactgatg ggaaaaatca tcggaatcac tgggggaatt gcctcaggta agtcaactgt
    24181 gacaaatttt ctaagacagc aaggctttca agcagtggat gccgacgcag tcgtccacca
    24241 actacagaaa cctggtggtc gtctgtttga ggctctagta cagcactttg ggcaagaaat
    24301 cattcttgaa aacggagaac tcaatcgccc tctcctagct agtctcatct tttcaaatcc
    24361 tgaagagcaa aaatggtcta atcaaattca aggggagatt atccgtgagg aactggctac
    24421 tttgagagaa cagttggctc agacagaaga gattttcttc atggatattc ccctactttt
    24481 tgagcaggac tacagcgatt ggtttgctga gacttggttg gtctatgtgg accgagatgc
    24541 ccaagtagaa cgcttaatga aaagggacca gttgtccaaa gatgaagctg agtctcgtct
    24601 ggcagcccag tggcctttag aaaaaaagaa agatttggcc agccaggttc ttgataataa
    24661 tggcaatcag aaccagcttc ttaatcaagt gcatatcctt cttgagggag gtaggcaaga
    24721 tgacagagat taactggaag gataatctgc gtattgcctg gtttggtaat tttctgacag
    24781 gagccagtat ttctttggtt gtacctttta tgcccatctt cgtggaaaat ctaggtgtag
    24841 ggagtcagca agtcgctttt tatgcaggct tagcaatttc tgtctctgct atttccgcgg
    24901 cgctcttttc tcctatttgg ggtattcttg ctgacaaata cggccgaaaa cccatgatga
    24961 ttcgggcagg tcttgctatg actatcacta tgggaggctt ggcctttgtc ccaaatatct
    25021 attggttaat ctttcttcgt ttactaaacg gtgtatttgc aggttttgtt cctaatgcaa
    25081 cggcactgat agccagtcag gttccaaagg agaaatcagg ctctgcctta ggtactttgt
    25141 ctacaggcgt agttgcaggt actctaactg gtccctttat tggtggcttt attgcagaat
    25201 tatttggcat tcgtacagtt ttcttactgg ttggtagttt tctattttta gctgctattt
    25261 tgactatttg ctttatcaag gaagattttc aaccagtagc caaggaaaag gctattccaa
    25321 caaaggaatt atttacctcg gttaaatatc cctatctttt gctcaatctc tttttaacca
    25381 gttttgtcat ccaattttca gctcaatcga tcggccctat tttggctctt tatgtacgcg
    25441 acttagggca gacagagaat cttctttttg tctctggttt gattgtgtcc agtatgggct
    25501 tttccagtat gatgagtgca ggagtcatgg gcaagctagg tgacaaggtg ggcaatcatc
    25561 gtctcttggt tgtcgcccag ttttattcag tcatcatcta tctcctctgt gccaatgcct
    25621 ctagccccct tcaactagga ctctatcgtt tcctctttgg cttgggaacc ggtgccttga
    25681 ttcccggggt taatgcccta ctcagcaaaa tgactcccaa agccggcatt tcgagggtct
    25741 ttgcctttaa tcaggtattc ttttatctgg gaggtgttgt tggtcccatg gcaggttctg
    25801 cagtagcagg tcaatttggc taccatgctg tcttttatgc gacaagcctt tgtgttgcct
    25861 ttagttgtct ctttaacctg attcaatttc gaacattatt aaaagtaaag gaaatctagt
    25921 gcgagtaaaa attaatctca aatgctcctc ttgtgacagt atcaattacc taaccagtaa
    25981 aaattcaaaa acccatccag acaagattga ggttttaaag tattgtccca aggaaagaaa
    26041 agtaacccta catcttgaat ctaagtagat tttatggtaa aataataggg attttaagga
    26101 gtttgatatg tataacctat tattaaccat tttattagta ttatctgttg tgattgtgat
    26161 tgcaattttc atgcaaccaa ccaaaaacca atccagcaat gtatttgatg ccagttcagg
    26221 tgatttgttt gaacgcagta aagctcgcgg ttttgaagct gtaatgcagc gtttgacagg
    26281 gattttagtc tttttctggc tagccattgc cttagcattg acggtattat caagtagata
    26341 agaaaataat gggcaggact aggtctttgc ctctttttat ttttaaagga tgtttgagaa
    26401 ggttttacag taaaagaaaa ttaaaaaatc tagaaagaaa atatgaaaga tagaataaaa
    26461 gaatatttac aagacaaggg aaaggtgact gttaatgatt tggctcaggc tttgggaaaa
    26521 gacagttcca aggattttcg tgagttgatt aaaaccttgt ccttaatgga aagaaagcac
    26581 cagattcgct ttgaagaaga tggtagtctg acattagaaa ttaagaaaaa acatgagatt
    26641 accctcaagg ggatttttca tgcccataaa aatggctttg gctttgttag tctggaaggc
    26701 gaggaggacg acctttttgt agggaaaaat gatgtcaact atgctattga tggtgatacc
    26761 gtcgaggtag tgattaagaa agtcgctgac cgcaataagg gaacagcagc agaagccaaa
    26821 attattgata tcctagaaca cagtttgaca acagttgtcg ggcaaatcgt tctggatcag
    26881 gaaaagccta agtatgctgg ctatattcgt tcaaaaaatc ataaaatcag tcaaccgatt
    26941 tatgttaaga aaccagccct aaaattagaa ggaacagaag ttctcaaagt ctttatcgat
    27001 aaatacccaa gcaagaaaca tgatttcttt gtcgctagtg tactcgatgt agtgggacac
    27061 tcaacggatg tcggaattga tgttcttgag gtcttggaat caatggacat tgtatctgag
    27121 tttccagaag ctgttgtcaa ggaagcagaa agtgtgcctg atgctccgtc tcaaaaggat
    27181 atggaaggtc gtctggatct aagagatgaa attaccttta ccattgacgg tgcggatgcc
    27241 aaggacttag acgatgcagt gcatatcaag gctctgaaaa atggcaatct ggagcttggg
    27301 gttcacatcg cagatgtttc ctattatgtg accgaggggt ctgcccttga caaggaagcc
    27361 cttaaccgtg cgacttctgt ttacgtgaca gaccgagtgg tgccaatgct tccagaacga
    27421 ctatcaaatg gcatctgctc tctcaatccc caagttgacc gcctgaccca gtctgctatt
    27481 atggagattg ataaacatgg tcgtgtggtc aactatacca ttacacaaac agttatcaag
    27541 accagttttc gtatgaccta tagcgatgtc aatgatatcc tagctggcga tgaagaaaag
    27601 agaaaagaat atcataaaat tgtaccaagt atcgaactca tggccaagct tcatgaaact
    27661 ttagaaaaca tgcgtgtgaa acgtggagct ctcaattttg ataccaatga agcgaagatt
    27721 ttagtggata aacaaggtaa gcctgttgat atcgttcttc ggcagcgtgg cattgccgag
    27781 cggatgattg agtcttttat gttgatggct aatgaaacag ttgccgaaca tttcagcaag
    27841 ttggatttgc cttttatcta tcgaattcac gaggagccta aggctgaaaa ggttcagaag
    27901 tttattgatt atgcttcgag ttttggcttg cgcatttatg ggactgccag tgagattagt
    27961 caggaggcac ttcaagacat catgcgtgct gttgagggag aaccttatgt agatgtattg
    28021 tccatgatgc ttcttcgctc tatgcagcag gctcgttatt cggagcacaa tcacggccac
    28081 tatggactag ctgctgacta ttatactcac tttaccagtc caattcgtcg ttatccagac
    28141 cttcttgttc accgtatgat tcgggattac ggctgttcta aggaaatagc agagcatttt
    28201 gaacaagtga ttccagagat tgcgacccag tcttccaacc gtgaacgtcg tgccatagaa
    28261 gctgagcgtg aagtcgaagc catgaaaaag gctgagtata tggaagaata cgtgggtgaa
    28321 gagtatgatg cagttgtatc aagtattgtc aaattcggtc tctttgtcga attgccaaac
    28381 acagttgaag gcttgattca catcactaat ctacctgaat tttatcattt caatgagcgt
    28441 gatttgactc ttcgtggaga aaaatcaggt atcactttcc gagtgggtca gcagatccgt
    28501 atccgtgttg aaagagcgga taaaatgact ggagagattg atttttcatt cgtacctagt
    28561 gagtttgatg tgattgaaaa aggcttgaaa cagtctagtc gtagtggcag agggcgtggt
    28621 tcaaatcgtc gttcggataa gaaggaagac aagagaaaat caggacgctc aaatgataag
    28681 cgtaagcatt cacaaaaaga caagaagaaa aaaggaaaga aaccttttta caaggaagta
    28741 gctaagaaag gagccaagca tggcaaaggg cgagggaaag gtcgtcgcac aaaataaaaa
    28801 ggcacgccac gactatacaa tcgtagatac gctagaggca gggatggtcc tgactggaac
    28861 tgaaatcaag agtgtacgag ctgctcgaat taatctcaag gatggctttg ctcaagtgaa
    28921 aaatggagaa gtttggctga gcaatgttca tatcgcgcct tacgaagagg gcaatatctg
    28981 gaaccaggaa ccagaacgtc gtcgtaaact cctgctccat aaaaagcaaa ttcaaaaatt
    29041 ggaacaagag accaaaggga caggaatgac cttagttccc cttaaggtct atataaaaga
    29101 tggctacgct aagcttcttt taggacttgc caaagggaag catgactatg acaaacggga
    29161 gtctatcaaa cgtcgtgagc aaaatcgaga tatcgcgcgt gtgatgaaag ctgttaatca
    29221 gcgataaaaa gaggaattga aaatggaaaa attagttgcc tataaacgca tgcctttgtg
    29281 gaataaacaa acaatgcctg aagctgttca gcaaaagcac aatacaaaag ttgggacttg
    29341 ggggaaaatt actgtcttga agggagctct caagtttatt gaattgacag aagaagggga
    29401 agttctagct gaacacctct ttgaagcagg ggcagacaat ccaatggccc aacctcaagc
    29461 ctggcaccga gtggaagctg ccacagatga tgtggaatgg tacttggaat tttattgtaa
    29521 acctgaggat tattttgcta aaaaatacaa taccaatcct gttcattcag aggtcctaga
    29581 ggccatgcag acagtgaaac aagggaaagc tttggatttg ggttgtggtc aggggcgtaa
    29641 ttctcttttt ctagcccagc aagattttga tgtgacggct gtagatcaaa atggactagc
    29701 tcttgaaatc ttgcaaagca ttgtggagca ggaagatttg gacatgcctg ttggccttta
    29761 cgatatcaat tcagctagca ttgaacaaga atatgatttt atcgtttcaa cagttgttct
    29821 catgtttcta caagcggacc gcattccagc tattattcaa aatatgcagg agaaaaccag
    29881 tgttggcggt tacaacctta tcgtttgtgc catggacacg gaggattatc cttgctcggt
    29941 taacttccca ttcaccttta aagaaggaga actggcagac tattacaagg attgggaatt
    30001 ggttaagtac aatgaaaatc caggccattt gcaccgtcgc gatgagaatg gcaatcgtat
    30061 tcaactacgc tttgcgacct tactagctaa gaaaatcaag taaacacaca tgaagattag
    30121 gaattttcct gatctttttt cttttttacg aatgatatag aaaaggaggg aattcatgtt
    30181 tgttgcgaga gatgctaggg gagaattggt aaatgtgtta gaggataaac ttgagaagca
    30241 agcatacacc tgcccagctt gtggaggcca gctccatttg cgtcaaggac caagtgtacg
    30301 gacgcatttt gcccataaat ccttaaaaga ctgtgatttt ttctttgaaa atgaaagtcc
    30361 agaacacctg gccaataagg aatccctcta tcactggttg aaaaaagaga caaaggttca
    30421 attagagtac ccgctttcag aacttaaaca gattgcggat gtatttgtaa atggcaatct
    30481 agctctagaa gttcagtgta gtcccttgcc tcagaaagtc cttaaagagc gaagtgaggg
    30541 ctatcgtagt cagggttacc aagtactgtg gttgctgggt caaaaactgt ggctcaagga
    30601 gcgtttgact cgtctacagc aaggttttct ttatttcagt caaaacatgg gcttttatgt
    30661 ttgggaatta gacaaggaaa aacaagtttt aagactcaaa tacctgattt accaggatct
    30721 ccgcggtaaa ctccattatc aaatcaagga attttcctat ggtcaaggta gtttattgga
    30781 aatattgcgt cttccctata agagacaaaa aatatctcat tttacagttt ctgaggacaa
    30841 ggacatctgt cgctatatcc ggcaacaact ttattatcaa aatctctttt ggatgaaaga
    30901 acaagcagaa gcctatcaaa agggagaaaa tatcctgact tatggactga aagaatggta
    30961 tccacaaatt cgaccaatag tgggcaaatt tttccagatt gaacaagact tgactagcta
    31021 ttatcagcac ttttatacct attaccaaaa aaatcctcaa aatgattggc aaaagcttta
    31081 tccaccagcc ttttatcagc aatatttctt gaaaaatatg gtagaataga aaggatggag
    31141 gaatctaatg gtattacaaa gaaatgaaat aaatgaaaaa gatacatggg atctatcaac
    31201 gatctaccca actgaccagg cttgggaaga agccttaaaa gatttaacag aacaattgga
    31261 gacagtagcc cagtatgaag gccatctctt ggatagtgcg gataacctac tagaaatcac
    31321 tgaattttct cttgaaatgg aacgccagat agagaagctt tacgtttatg ctcatatgaa
    31381 gaatgaccag gatacacgtg aagctaagta tcaagagtac tatgccaagg ccatgacact
    31441 ctacagccag ttagaccaag ccttttcatt ctatgagcct gaatttatgg agattagcga
    31501 aaagcagtat gctgactttt tagaagctca accaaagctg caggtttatc aacactattt
    31561 tgacaagctc ttgcaaggca aggatcacgt tctttcacaa cgtgaagaag aattattggc
    31621 tggagctgga gaaatctttg gttcagcaag tgaaaccttc gctatcttgg acaatgcgga
    31681 tattgtgttc ccttatgtcc tagacgatga tggtaaagaa gttcagctat ctcatgggac
    31741 ttacacacgt ttgatggagt ctaaaaaacg tgaggttcgc cgtggtgcct atcaagctct
    31801 ttatgctact tacgaacaat tccaacacac ctatgccaaa accttgcaaa ccaatgttaa
    31861 ggtgcaaaat taccgtgcta aagttcgtaa ctacaagagt gctcgtcatg cagccctcgc
    31921 agcgaatttt gttccagaaa gtgtttatga caatttggta gcagcagttc gcaagcattt
    31981 gccactctta catcgctatc ttgagcttcg ttcaaaaatc ttggggattt cagatctcaa
    32041 gatgtacgat gtctacacac cgctttcatc tgttgaatac agttttacct accaagaagc
    32101 cttgaaaaaa gcagaagatg ctttggcagt cttgggtgag gattacttga gccgtgttaa
    32161 acgtgccttc agcgagcgtt ggattgatgt ttacgaaaat caaggcaagc gttcaggtgc
    32221 ctactctggt ggttcttacg ataccaatgc ctttatgctt ctcaactggc aggacaatct
    32281 ggacaatctc tttactcttg ttcatgaaac aggtcacagt atgcattcaa gctatactcg
    32341 tgaaactcag ccttatgttt acggggatta ctctatcttt ttggctgaga ttgcctcaac
    32401 taccaatgaa aatatcttga cggagaaatt attggaagaa gtggaagacg acgcaacacg
    32461 ctttgctatt ctcaataact tcctagatgg tttccgtgga acagttttcc gccaaactca
    32521 atttgctgag tttgaacacg ccattcacca agcagatcaa aatggggagg tcttgacaag
    32581 cgatttccta aataaactct acgcagactt gaaccaagag tattatggtt tgagtaagga
    32641 agacaatcct gaaatccaat acgagtgggc tcgcattcca cacttctact ataactacta
    32701 tgtatatcaa tattcaactg gctttgcggc cgcctcagcc ttggctgaaa aaattgtcca
    32761 tggtagtcaa gaagaccgtg accgctatat cgactacctc aaggcaggta agtcggacta
    32821 tccacttaat gtcatgagaa aagctggtgt tgatatggag aaggaagact acctcaacga
    32881 tgcctttgca gtctttgaac gccgtttaaa tgagtttgaa gcccttgttg aaaaattagg
    32941 attggcataa aatggttgaa tcgtatagta agaatgctaa ccataacatg cgtcgtcctg
    33001 tcgtcaaaga agaaattgta gacttgatgc gtcagcgtca aaagcaggtc acaggtttct
    33061 tgaaagaatt ggaagacttt gcccgcaagg aaaatattcc tattattccc catgaaacgg
    33121 ttgcttattt ccgttttctt atggaaacca tgcagcctaa aaatattctg gaaattggga
    33181 cggctatcgg tttttcagct ctcttgatgg ctgaacatgc gccaaatgct aagattacaa
    33241 ctattgatcg taatccagaa atgattggtt ttgccaagga aaattttgcc cagtttgaca
    33301 gtcgcaagca aatcactctc ctagagggag atgcggtgga tgtcttatct acactgacag
    33361 agtcttatga tttcgtattt atggattctg ccaagtctaa atacatcgtc tttctgccag
    33421 aaatcctcaa acatttggaa gttggtggtg tggttgtctt ggatgatatt tttcaaggtg
    33481 gtgatgttgc caaggatatt atggaagtcc gtcgtggtca gcgaaccatt tatcgaggcc
    33541 ttcaaaaatt atttgatgca accttagaca atccagaact caccgcaaca ttagtgcctt
    33601 taggagatgg tattctcatg cttcgtaaaa atgtagcaga tgttcaactg tctgaaagcg
    33661 aatgattttc agaaaaattt aagaaaaaat agtaaaatag atagagtaac acttatctca
    33721 aaggagtaga catgaagaaa aaattattgg caggtgccat cacactatta tcagtagcaa
    33781 ctttagcagc ttgttcgaaa gggtcagaag gagcagacct tatcagcatg aaaggggatg
    33841 tcatcacaga acatcaattt tatgagcaag tgaaaaacaa cccttcagcc caacaagtct
    33901 tgttaaatat gaccatccaa aaagtttttg aaaaacaata tggctcagag cttgatgata
    33961 aagaggttga tgatactatt gccgaagaaa aaaaacaata tggcgaaaac taccaacgtg
    34021 tcttgtcaca agcaggtatg actcttgaaa cacgtaaagc tcaaattcgt acaagtaaat
    34081 tagttgagtt ggcagttaag aaggtagcag aagctgaatt gacagatgaa gcctataaga
    34141 aagcctttga tgagtacact ccagatgtaa cggctcaaat catccgtctt aataatgaag
    34201 ataaggccaa agaagttctc gaaaaagcca aggcagaagg tgctgatttt gctcaattag
    34261 ccaaagataa ttcaactgat gaaaaaacaa aagaaaatgg tggagaaatt acctttgatt
    34321 ctgcttcaac agaagtacct gagcaagtca aaaaagccgc tttcgcttta gatgtggatg
    34381 gtgtttctga tgtgattaca gcaactggca cacaagccta cagtagccaa tattacattg
    34441 taaaactcac taagaaaaca gaaaaatcat ctaatattga tgactacaaa gaaaaattaa
    34501 aaactgttat cttgactcaa aaacaaaatg attcaacatt tgttcaaagc attatcggaa
    34561 aagaattgca agcagccaat atcaaggtta aggaccaagc cttccaaaat atctttaccc
    34621 aatatatcgg tggtggagat tcaagctcaa gcagtagtac atcaaacgaa tagtccaaat
    34681 caatgagtca gggaaaaaac tctacttcag gaaaaaatga agcaaacatt cccacaataa
    34741 aacgcgtagt acaaggtttg tactgacccc caaaagttag acaaataatt tatccgaagg
    34801 atttagttct gtattgcaca gggcta
//
LOCUS       DAVLVF010000016        34417 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVLVF010000016 DAVLVF010000000
VERSION     DAVLVF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34417
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.016"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            584..1036
                     /locus_tag="VYN66_000716"
     CDS             584..1036
                     /locus_tag="VYN66_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1494777.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKAGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVQLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1055..2392
                     /locus_tag="VYN66_000717"
     CDS             1055..2392
                     /locus_tag="VYN66_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV1494778.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2471..3355
                     /locus_tag="VYN66_000718"
     CDS             2471..3355
                     /locus_tag="VYN66_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1494779.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWIINGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3355..4188
                     /locus_tag="VYN66_000719"
     CDS             3355..4188
                     /locus_tag="VYN66_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1494780.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4206..6299
                     /gene="nanB"
                     /locus_tag="VYN66_000720"
     CDS             4206..6299
                     /gene="nanB"
                     /locus_tag="VYN66_000720"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV1494781.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIGEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6311..7414
                     /locus_tag="VYN66_000721"
     CDS             6311..7414
                     /locus_tag="VYN66_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1494782.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7588..8286
                     /locus_tag="VYN66_000722"
     CDS             7588..8286
                     /locus_tag="VYN66_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1494783.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8305..9822
                     /locus_tag="VYN66_000723"
     CDS             8305..9822
                     /locus_tag="VYN66_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1494784.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALAVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETIAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9947..11275
                     /locus_tag="VYN66_000724"
     CDS             9947..11275
                     /locus_tag="VYN66_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1494785.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11527..12393
                     /locus_tag="VYN66_000725"
     CDS             11527..12393
                     /locus_tag="VYN66_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1494786.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAIMIAIVSF
                     VQFKVLGNDVEY"
     gene            12409..13248
                     /locus_tag="VYN66_000726"
     CDS             12409..13248
                     /locus_tag="VYN66_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1494787.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13520..13972
                     /locus_tag="VYN66_000727"
     CDS             13520..13972
                     /locus_tag="VYN66_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1494788.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNRFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14069..14413
                     /locus_tag="VYN66_000728"
     CDS             14069..14413
                     /locus_tag="VYN66_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494789.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMAWIIRGIVTALIGRYIKLGTY
                     AVKYGISMARSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDA
                     HDKIPNNGRINF"
     gene            14700..15344
                     /locus_tag="VYN66_000729"
     CDS             14700..15344
                     /locus_tag="VYN66_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1494790.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15513..16430
                     /locus_tag="VYN66_000730"
     CDS             15513..16430
                     /locus_tag="VYN66_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV1494791.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTSSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTAAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEDDRPVVEAAAALIRETKERFL"
     gene            16448..17332
                     /locus_tag="VYN66_000731"
     CDS             16448..17332
                     /locus_tag="VYN66_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1494792.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17348..18199)
                     /locus_tag="VYN66_000732"
     CDS             complement(17348..18199)
                     /locus_tag="VYN66_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1494793.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKNSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18399..20456
                     /gene="pbp2b"
                     /locus_tag="VYN66_000733"
     CDS             18399..20456
                     /gene="pbp2b"
                     /locus_tag="VYN66_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV1494794.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20467..21063
                     /gene="recR"
                     /locus_tag="VYN66_000734"
     CDS             20467..21063
                     /gene="recR"
                     /locus_tag="VYN66_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV1494795.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDSTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21239..22282
                     /locus_tag="VYN66_000735"
     CDS             21239..22282
                     /locus_tag="VYN66_000735"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV1494796.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDSIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22366..23739
                     /locus_tag="VYN66_000736"
     CDS             22366..23739
                     /locus_tag="VYN66_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV1494797.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPIDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     SLENECK"
     gene            23726..24337
                     /locus_tag="VYN66_000737"
     CDS             23726..24337
                     /locus_tag="VYN66_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1494798.1"
                     /translation="MNASDFAKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQVSDLDADEVAELLKPTSAYATPLMDVRAWIVEDEKICLVRGQGENDWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24428..25132
                     /locus_tag="VYN66_000738"
     CDS             24428..25132
                     /locus_tag="VYN66_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV1494799.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYRENK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25351..26724
                     /gene="ftsA"
                     /locus_tag="VYN66_000739"
     CDS             25351..26724
                     /gene="ftsA"
                     /locus_tag="VYN66_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV1494800.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26741..28000
                     /gene="ftsZ"
                     /locus_tag="VYN66_000740"
     CDS             26741..28000
                     /gene="ftsZ"
                     /locus_tag="VYN66_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV1494801.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            28005..28676
                     /locus_tag="VYN66_000741"
     CDS             28005..28676
                     /locus_tag="VYN66_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV1494802.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28686..29225
                     /locus_tag="VYN66_000742"
     CDS             28686..29225
                     /locus_tag="VYN66_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV1494803.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29225..29488
                     /locus_tag="VYN66_000743"
     CDS             29225..29488
                     /locus_tag="VYN66_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV1494804.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29485..30270
                     /locus_tag="VYN66_000744"
     CDS             29485..30270
                     /locus_tag="VYN66_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV1494805.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30279..31067
                     /gene="divIVA"
                     /locus_tag="VYN66_000745"
     CDS             30279..31067
                     /gene="divIVA"
                     /locus_tag="VYN66_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV1494806.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31415..34207
                     /locus_tag="VYN66_000746"
     CDS             31415..34207
                     /locus_tag="VYN66_000746"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV1494807.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPASETRKFVVAAELLTSLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGQVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10591 a         6073 c         7487 g        10266 t
ORIGIN      
        1 aagtatactc taattgaaaa cggtaacaaa actaatttag agaatgaaat atagagtatt
       61 tctctcttaa aagtttttgg tgaaacgaga tgtagaaagg agatttagcc aaagagtcta
      121 ttagtgctag aataatagat tagaattatt ttagaaaaac gaagtgagca gcttataaat
      181 tcaagtcccc aaatagattc atactagtat cttttgcaaa aaataaaggg cgacttcctt
      241 catgaatatc aatttcatct ataaggaagg tagctaattg aactaactta tttattctgt
      301 ttgtcgctag aaaaatcaga cctccttgtg aagattgagg agatacttaa tgaaaatcaa
      361 agaagaaact agcaagctag tagcagattg cccaaaacac cgctttgagg ttgtagataa
      421 gactgacgta tataatccaa ggtgaagcga ctgtggtttg aagagatttt caaagagtat
      481 aggctagaga gtagtgtttt tatgtccttc tagtagaaaa tgctagacag aagaatgggg
      541 aacttggata ggagaaatag attgagaaag gaggttagaa gagatgatta ttacaaaaat
      601 tagccgttta ggaacttatg tgggagtaaa tccacatttt gcaacattaa tagattttct
      661 agaaaaagca ggactagaaa atttaacaga aggttcgatt gctatcgatg gtaatcgatt
      721 gtttgggaat tgctttactt atctagcaga tggtcaagca ggggctttct ttgaaacaca
      781 ccaaaaatat ttggatattc atttagtttt ggaaaacgaa gaagccatgg ctgttacatc
      841 gccggaaaat gtaagcgtta cccaagaata tgatgaagag aaagatattg aattatacac
      901 agggaaagtg gaacagttgg ttcagttgag agctggcgaa tgcctcatca cttttccaga
      961 agatttacat caacccaagg ttcgtataaa tgatgaacct gtgaaaaaag ttgtctttaa
     1021 agttgcgatt tcttaatgta gaaagagaag aacgatgaaa aaaatgagaa agtttttatg
     1081 tctagctgga attgcgctag cggctgttgc cttggtagct tgttcaggaa aaaaagaagc
     1141 tacaactagt actgaaccac caacagaatt atctggtgag attacaatgt ggcactcctt
     1201 tactcaagga ccccgtttag aaagtattca aaaatcagca gatgctttca tgcaaaagca
     1261 tccaaaaacg aaaatcaaga ttgaaacatt ttcttggaat gacttctata ctaaatggac
     1321 tacaggttta gcaaatggaa atgtgccaga tatcagtaca gctcttccta accaagtaat
     1381 ggaaatggtc aactcagatg ctttggttcc gctaaatgat tctatcaagc gtattggaca
     1441 agataaattt aacgaaactg ccttaaatga agcaaaaatc ggagatgatt actactctgt
     1501 tcctctttat tcacatgcac aagtcatgtg ggttagaaca gatttgttaa aagaacataa
     1561 tattgaggtt cctaaaactt gggatcaact ctatgaagct tctaaaaaat tgaaagaagc
     1621 tggagtttat ggcttgtctg ttccgtttgg aacaaatgac ttaatggcaa cacgtttctt
     1681 gaacttctac gtacgtagtg gtggaggaag cctcttaaca aaagatctta aagcagactt
     1741 gacaagccaa cttgctcaag atggtattaa atactgggtt aaattgtata aagaaatctc
     1801 acctcaagat tctttgaact ttaatgtcct tcaacaagct accttgttct atcaaggaaa
     1861 aacagcattt gactttaact ctggcttcca tatcggagga attaatgcca acagtcctca
     1921 attgattgat tcgattgatg cttatcctat tccaaaaatc aaagagtctg ataaagacca
     1981 aggaattgaa acctcaaaca ttccaatggt tgtttggaaa aattcaaaac atccagaagt
     2041 tgctaaagca ttcttagaag cactttataa tgaagaagac tacgttaaat tccttgattc
     2101 aactccagta ggtatgttgc caactattaa ggggattagc gattctgcag cctataaaga
     2161 aaatgaaact cgtaagaaat ttaaacatgc tgaagaagta attactgaag ctgttaaaaa
     2221 aggtactgct attggttatg aaaatggacc aagtgtacaa gctggtatgt tgactaacca
     2281 acacattatt gaacaaatgt tccaagatat cattacaaat ggaacagatc ctatgaaagc
     2341 agcaaaagaa gcagaaaaac aattaaacga tttatttgag gctgttcagt agatgtaaaa
     2401 gactagaaaa taggtgggct agtgagctga aaagctctag cccaatcttg taaaagaagg
     2461 gagaaggaga atggttaaag aacgtaattt aactcgctgg atatttgttt tgccagctat
     2521 gattatcgta ggattactct ttgtttatcc gtttttctcg agtatttttt atagctttac
     2581 caataagcat ttgattatgc ctaattataa atttgttggt ttggctaact ataaagctgt
     2641 gctatcagat cccaacttct ttaatgcgtt ctttaattca attaagtgga ccgttttctc
     2701 attagttggt caagttttag tagggtttgt attggcttta gctcttcaca gagtacgcca
     2761 cttcaagaaa ttatatagga ccttattgat tgttccttgg gcatttccta ccatcgttat
     2821 tgccttctct tggcagtgga ttataaacgg ggtttatggc tacttaccta atctaatcgt
     2881 aaaattaggt ttaatggaac atacacctgc atttttgaca gatagtacat gggcattcct
     2941 atgtttggtg tttatcaaca tttggtttgg agcaccaatg attatggtta atgtgctttc
     3001 agctttgcaa acagtaccag aagaacaatt tgaggctgct aagatagatg gtgcttcaag
     3061 ttggcaggtg ttcaagttta tcgtctttcc acatattaaa gtggttgtag gacttctagt
     3121 tgttttgaga actgtatgga tctttaataa ctttgacatt atctacctca ttactggtgg
     3181 tggaccagcc aatgctacaa cgacgcttcc aatttttgct tacaacctgg gttggggaac
     3241 taaattgttg ggtcgtgctt cagcagttac agtattgctc tttatcttct tggtggcgat
     3301 ttgctttatc tactttgcta tcatcagtaa gtgggaaaag gagggtagaa aataatgaag
     3361 aagaaatcca gtatttattt agatattctc tcacatgtac ttttagttgg tgcgaccatc
     3421 gttgcaattt tcccattggt atggattatc atatcttctg tcaaagggaa aggggaatta
     3481 actcagtatc caacacgatt ttggcctgaa cagtttacat tagattattt cactcatgtt
     3541 atcaacgatt tgcacttcat tgataacatt cgaaacagtt taatcattgc cttggctaca
     3601 accctcattg cgattattat ttctgctatg gcagcctatg gtattgttcg attctttcct
     3661 aaattgggag caatcatgtc gagactactc gtcattacct acattttccc accaattttg
     3721 ttagcaattc cctattcaat tgccattgct aaagttgggt taacaaatag tttatttggc
     3781 ttgatgatgg tttatctatc ttttagtgtt ccatatgcag tttggctctt agttggattt
     3841 ttccaaacag ttccaattgg aattgaagaa gcggctagaa ttgatggtgc aaataaattt
     3901 gttacgtttt ataaagttgt gctaccgatt gtagcaccag gtattgtagc aacagctatt
     3961 tatacattta tcaatgcttg gaatgaattc ctgtatgcct tgattttgat taacaataca
     4021 ggaaagatga cagtagcagt agcccttcgt tcacttaatg gttcagaaat actagactgg
     4081 ggagatatga tggcagcgtc tgttattgta gttcttccat caattatttt cttctctatc
     4141 atccaaaata agattgcaag tggattatca gaaggatctg tgaagtagac gaaagaagga
     4201 aaaaaatgaa taaaagaggt ctttattcaa aactaggaat ttctgttgta ggcattagtc
     4261 ttttaatggg agtccccact ttgattcatg cgaatgaatt aaactatggt caactgtcca
     4321 tatctcctat ttttcaagga ggctcatatc aactgaacaa taagagtata gatatcagcc
     4381 ctttgttatt agataaattg tctggagaga gtcagacagt agtaatgaaa tttaaagcag
     4441 atgaaccaaa ctctcttcaa gctttgtttg gcctatctaa tagtaaagca ggctttaaaa
     4501 ataattactt ttcaattttc atgagagatt ctggtgagat aggtgtagaa ataagagacg
     4561 cccaaaaggg aataaattat ttattttcca gaccagcttc attatgggga aaacataaag
     4621 gacaggcagt tgaaaataca ctagtatttg tatctgattc taaagataaa acatacacaa
     4681 tgtatgttaa tggaatagaa gtgttctctg aaacagttga tacatttttg ccaatttcaa
     4741 atataaatgg tatagataag gcaacactag gagctgttaa tcgtgaaggt aaggaacatt
     4801 acctcgcaaa aggaagtatt ggtgaaatca gtctatttaa caaagcaatt agtgatcagg
     4861 aagtttcaac tattccctta tcaaatccat ttcagttaat tttccaatca ggagattcta
     4921 ctcaagctaa ctattttaga ataccgacac tatatacatt aagtagtgga agagttctat
     4981 caagtattga tgcacgttat ggtgggactc atgattctaa aagtaagatt aatattgcca
     5041 cttcttatag tgatgataat gggaaaacgt ggagtgagcc aatttttgct atgaagttta
     5101 atgactatga ggagcagtta gtttactggc cacgagataa taaattaaag aatagtcaaa
     5161 ttagtggaag tgcttcattc atagattcat ccattgttga agataaaaaa tctgggaaaa
     5221 cgatattact agctgatgtt atgcctgcgg gtattggaaa taataatgca aataaagccg
     5281 actcaggttt taaagaaata aatggtcatt attatttaaa actaaagaag aatggagata
     5341 acgatttccg ttatacagtt agagaaaatg gtgtcgttta tgatgaaaca actaataaac
     5401 ctacaaatta tactataaat gataagtatg aagttttgga gggaggaaag tctttaacag
     5461 tcgaacaata ttcggttgat tttgatagtg gctctttaag agaaaaacat aatggaaaac
     5521 aggtccctat gaatgttttc tacaaagatt cgttatttaa agtgactcct actaattata
     5581 tagcaatgac aactagtcag aatagaggag agagttggga acaatttaag ttgttgcctc
     5641 cgttcttagg agaaaaacat aatggaactt acttatgtcc cggacaaggt ttagcattaa
     5701 aatcaagtaa cagattgatt tttgcaacat atactagtgg agaactaacc tatctcattt
     5761 ctgatgatag tggtcaaaca tggaagaaat cctcagcttc aattccgttt aaaaatgcaa
     5821 cagcagaagc acaaatggtt gaactgagag atggtgtgat tagaacattc tttagaacca
     5881 ctacaggtaa gatagcttat atgactagta gagattctgg agaaacatgg tcggaagttt
     5941 cgtatattga tggaatccaa caaacttcat atggcacaca agtatctgca attaaatact
     6001 ctcaattaat tgatggaaaa gaagcagtca ttttgagtac accaaattct agaagtggcc
     6061 gtaagggagg tcaattagtt gtcggtttag tcaataaaga agatgatagt attgattgga
     6121 aataccacta tgatattgat ttgccttcgt atggttatgc ctattctgcg attacagaat
     6181 tgccaaatca tcacataggt gtactgtttg aaaaatatga ttcgtggtcg agaaatgaat
     6241 tgcatttaag caatgtagtt cagtatatag atttggaaat taatgattta acaaaataaa
     6301 ggagaaaaac atggttaaat acggtgttgt tggagcaggg tattttggag ctgaattggc
     6361 tcgctacatg caaaagaatg atggagcaga gattactctt ctctatgatc cagataatgc
     6421 agaggcgatt gcagaagaat tgggagcaaa agtagcaagt tccttagatg agttggtttc
     6481 tagcgatgaa gtagattgtg ttatcgtcgc aactccaaat aatcttcata aggaaccggt
     6541 tattaaggct gcacagcatg gtaaaaatgt tttctgtgaa aaaccaattg cgctttctta
     6601 tcaagattgt cgcgagatgg tagatgcgtg taaagaaaac aatgtaacct ttatggcagg
     6661 acatattatg aatttcttta atggtgttca tcatgcaaaa gaactcatta atcaaggagt
     6721 tatcggagac gttctatatt gtcatacagc tcgtaatggt tgggaagaac aacaaccgtc
     6781 agtatcatgg aaaaaaattc gtgaaaaatc aggtggtcac ttgtatcacc acatccatga
     6841 attggattgc gttcaattcc ttatgggggg catgcctgaa actgtaacca tgacaggtgg
     6901 aaatgtggcc catgaaggtg aacatttcgg tgatgaagat gatatgattt ttgtcaatat
     6961 ggaattttct aataagcgtt ttgctttgtt agaatggggt tcagcttatc gttggggtga
     7021 acattatgtc ttaatccaag gaagcaaagg tgccatccgc ttagacttat tcaactgtaa
     7081 aggaactctt aagctagatg ggcaagaaag ctatttcttg attcacgaat cgcaagaaga
     7141 agatgatgat cggactcgta tctatcatag tacagagatg gatggagcaa ttgcttatgg
     7201 taaaccaggt aaacgtactc cattatggct atcatctgtc attgataaag aaatgcgcta
     7261 tctgcatgag attatggaag gagctccagt atcagaagaa tttgcaaaac ttttgacagg
     7321 tgaagctgcc ctagaagcaa ttgctactgc agatgcttgt acccagtcta tgtttgaaga
     7381 tcgcaaagta aaattgtcag aaattgtaaa ataaattttg gtattctcct atttataggt
     7441 cgacttgctc ctctgaaagt acttttagag gagctgtttg actttgctag tttttgaaac
     7501 tgaaatctat tatactacaa actattgaaa gcgttttaat tttaaggtat aataatctca
     7561 tagaaataaa gaaaaggagg aaagaggatg ccacagatta gcaaagaagc cttgattgag
     7621 caaatcaaag atggaatcat cgtttcttgt caggctcttc ctcatgaacc gctttataca
     7681 gaagcgggag gggtgattcc cttgctggtc aaagcggctg agcaaggtgg agcagtcggt
     7741 atccgagcaa acagtgttcg cgatatcaag gaaattaagg aggtcactaa acttccaatc
     7801 attgggatta tcaaacgtga ttatccacct caggaaccct tcatcacggc tactatgaaa
     7861 gaagttgatg aattggcaga actggacatc gaggtgattg ctctggattg taccaagcgt
     7921 gaacgctacg atggtttgga aattcaagag ttcattcgtc aggttaagga gaaatatcct
     7981 aatcagcttt tgatggctga tactagtatc ttcgaagaag ggctagcagc tgtagaagca
     8041 ggaattgact ttgtcggaac aaccttatca ggctacacat cctacagtcc aaaagtagac
     8101 ggtccagatt ttgaattgat taagaaactc tgtgatgccg gtgtagatgt cattgcagaa
     8161 ggaaaaattc atacaccaga acaagccaaa caaatccttg aatatggagt gcgaggcatc
     8221 gttgttggtg gcgccattac tagaccaaaa gagattacag aacgcttcgt tgctagtctt
     8281 aaataagatg tgagggggag ttttatgttt aaagttttac aaaaagttgg aaaagctttt
     8341 atgttaccta tagctatact tcctgcagca ggtctacttt tggggattgg tggtgcactt
     8401 tcaaacccaa ccacgatagc aacttatcca atactagaca atagtatttt tcaatcaata
     8461 ttccaagtaa tgagctctgc aggagaggtt gtattcagta atttgtcact acttctctgt
     8521 gtgggattat gtattggctt agcgaaacga gataaaggaa ccgctgcgtt agcaggagta
     8581 actggttact tagttatgac tgcaacgatc aaagctttgg taaaactttt tatggcagaa
     8641 ggatctgcaa ttgatactgg agttattgga gcattagctg tcggaatagt tgccgtatat
     8701 ttgcacaacc gatataacaa tattcaatta ccttccgctt taggattctt tggaggttca
     8761 cgcttcgttc ctattgttac atcgttctct tctatcttga ttggctttgt cttctttgtt
     8821 atttggccac ctttccaaca acttcttgtt tctacaggtg gatatatttc tcaggcgggt
     8881 ccaattggaa cttttctata tggattttta atgagacttt ctggagcagt aggcttacat
     8941 catataattt accctatgtt ttggtatact gaacttggtg gtgttgaaac tattgcagga
     9001 caaacagtgg ttggagctca aaaaatattt tttgctcaat tagccgatcc ggcccattct
     9061 ggattattta cagaaggaac aaggtttttt gcaggtcgtt tctcaacaat gatgttcggt
     9121 ttaccggctg cctgtttagc gatgtaccat agtgttccta aaaatcgtcg taaaaaatac
     9181 gcgggtttgt tttttggagt tgctttaaca tcttttatta ccggtattac agaaccaatt
     9241 gaatttatgt ttctattcgt cagtccggtt ctatatgttg ttcacgcatt ccttgatggt
     9301 gttagcttct ttattgcaga cgtcttaaat atttcaatag gaaacacatt ttcaggaggt
     9361 gtaatcgatt tcactttatt tggaattttg caggggaacg ctaagacgaa ttgggttctt
     9421 cagattccaa ttggacttat ttggagtgtt ttgtattata ttatttttag atggttcatt
     9481 actcaattca atgttctaac gccaggacga ggagaagaag tagattctaa agaaatttct
     9541 gaatccgcag attcaacttc aaatactgca gattatttaa aacaggatag cctacaaatt
     9601 atcagagcct tgggtggatc aaataatata gaagatgtag atgcttgtgt gacacgttta
     9661 cgtgtagctg taaaagaagt taatcaagtt gataaagcac ttttaaaaca aattggtgca
     9721 gttgatgtct tagaagtgaa gggtggcatt caagcaatct atggagcaaa agcaatctta
     9781 tataaaaata gtattaatga aattttaggt gtagatgatt aagtacttac tgacttaata
     9841 aaaaacagag gagagtgatg gatgagtagg atgaaatgaa atcgcataca agaaataaag
     9901 aactcattat ccaagttgga tacgcttatt acataggaga atacaaatga aatttagaaa
     9961 attagcttgt acagtacttg cgggtgctgc ggttcttggt cttgctgctt gtggcaattc
    10021 tggcggaagt aaagatgctg ccaaatcagg tggtgacggt gccaaaacag aaatcacttg
    10081 gtgggcattc ccagtattta cccaagaaaa aactggtgac ggtgttggaa cttatgaaaa
    10141 atcaatcatc gaagcgtttg aaaaagcaaa cccagatata aaagtgaaat tggaaaccat
    10201 cgacttcaag tcaggtcctg aaaaaatcac aacagccatc gaagcaggaa cagctccaga
    10261 cgtactcttt gatgcaccag gacgtatcat ccaatacggt aaaaacggta aattggctga
    10321 gttgaatgac ctcttcacag atgaatttgt taaagatgtc aacaatgaaa acatcgtaca
    10381 agcaagtaaa gctggagaca aggcttatat gtatccgatt agttctgccc cattctacat
    10441 ggcaatgaac aagaaaatgt tagaagatgc tggagtagca aaccttgtaa aagaaggttg
    10501 gacaactgat gattttgaaa aagtattgaa agcacttaaa gacaagggtt acacaccagg
    10561 ttcattgttc agttctggtc aagggggaga ccaaggaaca cgtgccttta tctctaacct
    10621 ttatagcggt tctgtaacag atgaaaaagt tagcaaatat acaactgatg atcctaaatt
    10681 cgtcaaaggt cttgaaaaag caactagctg gattaaagac aatttgatca ataatggttc
    10741 acaatttgac ggtggggcag atatccaaaa ctttgccaat ggtcaaacat cttacacaat
    10801 cctttgggca ccagctcaaa atggtatcca agctaaactt ttagaagcaa gtaaggtaga
    10861 agtggtagaa gtaccattcc catcagacga aggtaagcca gctcttgaat accttgtaaa
    10921 cgggtttgca gtattcaaca ataaagacga caagaaagtc gctgcatcta agaaattcat
    10981 ccagtttatc gcagatgaca aggagtgggg acctaaagac gtagttcgta caggtgcttt
    11041 cccagtccgt acttcatttg gaaaacttta tgaagacaaa cgcatggaaa caatcagcgg
    11101 ctggactcaa tactactcac catactacaa cactattgat ggatttgctg aaatgagaac
    11161 actttggttc ccaatgttgc aatctgtatc aaatggtgac gaaaaaccag cagatgcttt
    11221 gaaagccttc actgaaaaag cgaacgaaac aatcaaaaaa gctatgaaac aatagtcctt
    11281 agttattcta taaaaagtag ttttttaaag aacctaagag tgtatacccc cttttccctc
    11341 tacacagata gtgtaagaaa agggggcttt tgtttaaaat gtaagaaact gtcacgaaat
    11401 taaaatgaag ttcttacata agcgaatcat aaaaaatttc attttgattt taaaacagtt
    11461 caagaaagtc aaaaaattat tctatttgaa agagaggtgc cgactgtgaa agtcaataaa
    11521 atccgtatgc gggaaacagt gatttcctac gctttcctag caccagtatt attcttcttt
    11581 gtcatctttg tgttggctcc gatggtgatg ggcttcatta caagtttctt taactactca
    11641 atgactaaat ttgagtttgt aggcttggat aactatatcc gtatgtttaa agatcctgtc
    11701 tttacaaaat ctctgattaa cacagttatt ttggttattg gatctgtacc agttgttgtt
    11761 ctattctcac tctttgtagc atctcagacc tatcatcaaa atgtcattgc cagatccttc
    11821 taccgtttcg tcttcttcct tcctgttgta acgggtagtg ttgccgtgac agttgtttgg
    11881 aaatggattt atgacccact atcagggatt ctaaactttg tccttaagtc cagccacatc
    11941 atcagccaaa acatttcttg gttgggagat aaaaactggg cattgatggc gattatgatt
    12001 attctcttga ccacttcagt tggtcagccc atcatccttt atatcgctgc catggggaat
    12061 attgacaatt cactggttga agcggcgcgt gttgatggtg caactgagtt tcaagttttt
    12121 tggaagatta aatggccaag ccttcttcca acaactcttt atattgcaat catcacaaca
    12181 attaactcat tccagtgttt tgccttgatt cagcttttga catctggtgg tccaaactac
    12241 tcaacaagta ccttgatgta ctacctttac gaaaaagcct tccaattgac agaatacggc
    12301 tatgccaaca caattggtgt cttcttggca atcatgattg ctatcgtaag ctttgttcaa
    12361 tttaaagtac ttggaaacga cgtagaatac taaagaaagg agacagctat gcaatctaca
    12421 gaaaaaaaac cattaacagc ctttactgtt atttcaacaa tcattttgct cttgttgact
    12481 gtgctgttca tctttccatt ctactggatt ttgacagggg cattcaaatc acaacctgat
    12541 acaattgtta ttcctcctca gtggttccct aaaatgccaa ccatggaaaa cttccaacaa
    12601 ctcatggtgc agaaccctgc cttgcaatgg atgtggaact cagtatttat ctcattggta
    12661 accatgttct tagtctgtgc aacctcatct ctagcaggtt atgtattggc taaaaaacgt
    12721 ttctatggtc aacgcattct atttgctatc tttatcgctg ctatggcgct tccaaaacaa
    12781 gttgtccttg taccattggt acgtatcgtc aacttcatgg gaatccacga tactctctgg
    12841 gcagttatct tgcctttgat tggatggcca ttcggtgtct tcctcatgaa acagttcagt
    12901 gaaaatatcc ctacagagtt gcttgaatca gctaaaatcg acggttgtgg tgagattcgt
    12961 accttctgga gtgtagcctt cccgattgtg aaaccagggt ttgcagccct tgcaatcttt
    13021 accttcatca atacttggaa tgactacttc atgcaattgg taatgttgac ttcacgtaac
    13081 aatttgacca tctcacttgg ggttgcgacc atgcaggctg aaatggcaac caactatggt
    13141 ttgattatgg caggagctgc ccttgctgct gttccaatcg tcacagtctt cctagtcttc
    13201 caaaaatcct tcacacaggg tattactatg ggagcggtca aaggataata ctctgagaaa
    13261 atctcttcaa actacgtcag cgtcaccttg ccatacttaa gtattgcctg cggttagctt
    13321 cctagtttgt tcttcaattt tcattgagta taggaaaatc aatctatcaa gatacagaag
    13381 tatattttat agatttagag aatatagagg ttataagtgt ctacaaaatg gagggtatgc
    13441 agttacttta tgaagttttg tcagacactt ataaacttaa gaatggtttt agttaactat
    13501 cagaaacgaa ggaaagagta tgatttttga cgatttgaaa aacatcacct tttacaaagg
    13561 gattcatccc aatttagaca aggctatcga ctatctctac caacatcgta aagattcatt
    13621 cgaattagga aagtatgaga ttgatggaga taaagtcttt ctagttgttc aggaaaatgt
    13681 cctcaatcaa gttgagaata atcgatttga acaccataag aactatgcag atttgcattt
    13741 gctgatagaa gggcatgaat attcgagcta cggttcacgt atcaaagacg aggcagtagc
    13801 attcgacgaa gcgagtgaca ttggctttgt tcattgtcat gaacactacc cactcttgtt
    13861 gggttatcac aattttgcga ttttcttccc aggtgagcca catcagccaa atggttatgc
    13921 aggcatggaa gaaaaggttc gaaaatatct ctttaaaatt ttgattgatt aaaaatagga
    13981 tgaattgttt ttttgtaaag ctttgataat actctaccat gaaattgatc tttgtgaggt
    14041 agagaaatga gaataaaata tttaaaaatt ggtatcttct aagtatgctg caagagctag
    14101 tttcttagat ggacagggga ttacagttga tgagatggct tggataatta ggggcattgt
    14161 gactgcattg attggtagat acataaaatt aggtacttat gcagttaagt atggtattag
    14221 tatggcgcgc tcgatcttaa gtagggcagc tgcaactgcg gcagcaagag taggattatt
    14281 gactaagatt tttggatgga ttttacgaat agctgtgctc gcagctgatg tatatggtaa
    14341 ttttgccaac aatattgctg tagcttggga tgcacatgat aaaatcccta acaatggtcg
    14401 tataaacttt taaaatgcga gaatgaaagc actttgtatt tttttattgt atatgttagc
    14461 ttggacagtg cttgcaatga taatccgtgg agggctagat ggatttgatg aacacacttg
    14521 gcgtactatt ttaattgctt cgctgtccgg gatatatata gattatatgt ccatagataa
    14581 aagtagaaaa aagtccaaaa gaaaaaagaa aaaatagatt tgttcatggt agggacttat
    14641 gaaagcttta ctgacaaaaa agaaaacagt ttacaaagaa aaatgatgga ggagcaaaca
    14701 tggcacaaaa aggagtaagc cttatcaagg cagcatttga tacagataac tttctcatgc
    14761 gttttagtga gaaagtcttg gacatcgtga cagccaatct tctttttgtc gtctcttgtt
    14821 tgcccatcgt gacgattgga gtggctaaaa tcagcctcta cgagaccatg ttcgaagtta
    14881 agaagagcag acgggtgcct gtttttaaaa tctatctaag atctttcaag caaaatctga
    14941 aactaggtct tcagctgggt ttaatggagt taggaattgt gtttcttacc ctttcagatc
    15001 tctatctttt ctggggtcaa acagctctgc ccttccaatt gctgaaagcc atttgtctag
    15061 gcattctgat tttccttacg attgtgatgt tggctagcta tccaatcgcg gcacgttatg
    15121 atttaacttg gaaagaagtg ttacaaaaag ggcttatctt ggctagtttt aactttcctt
    15181 ggttcttcct catgttagcc attcttgtcc tcattgtgat ggttctttat ctgtccgcct
    15241 tcagtctact cttaggtggc tcagtcttcc tactttttgg gtttggacta ttggtcttta
    15301 tccagactgg attgatggag aaaattttcg caaaatacca ataggagcct tatttctgaa
    15361 actactttca aaggctccaa acgctattct ataagcgaga aactaaaatc taagtgtcta
    15421 taagatattg aaagcctttt cggtaaagtg tatactaaag aagtaaagaa aaatctgctt
    15481 tagcagaaaa taaaaataat aggagaaaat ctatgtcaga tttgaaaaaa tacgaaggtg
    15541 tcattccagc cttctacgca tgttatgatg agcaaggaga agtaagtcca gagcgtacgc
    15601 gtgccttggt tcaatacttc attgataaag gtgttcaagg tctttatgtc aatggttctt
    15661 ctggtgaatg tatctaccaa agcgttgaag atcgcaagtt gattttggaa gaagtcatgg
    15721 cggtagccaa aggtaaattg accattattg cccatgttgc ttgcaataat actaaagata
    15781 gtatggaact tgctcgccat gctgaaagct tgggagtaga tgctattgca acgattccac
    15841 caatttactt ccgcttgcca gaatactcag ttgccaaata ctggaacgat attagttctg
    15901 cagctccaaa cacagactac gtgatttaca acattcctca attggcaggg gttgctttga
    15961 cttcaagcct ttacacagaa atgttgaaaa atcctcgtgt tatcggtgtt aagaactctt
    16021 caatgccagt tcaagatatc caaacctttg tcagccttgg cggggaagac catattgtct
    16081 ttaacggtcc agatgaacag ttcctaggtg gccgcctcat gggtgctaag gctggtatcg
    16141 gtggtactta tggtgctatg ccagaactct tcttgaaact caatcagttg attgcggata
    16201 aagatttaga aacagcgcgt gaattgcagt atgctatcaa cgcaatcatt ggtaaactca
    16261 ctgctgcaca tggaaatatg tacggtgtca tcaaagaagt tttgaagatc aatgaaggct
    16321 tgaatattgg ttctgttcgt tcaccattga caccagtgac tgaagacgat cgtccagttg
    16381 tagaagcggc tgcagccttg attcgtgaaa ccaaggagcg cttcctctaa tctaaaagga
    16441 ggtatttatg acatactacg ttgcaattga tatcggtgga actaacatca agtatggttt
    16501 ggttgatcaa gaggggcaac ttcttgaatc acatgaaatg ccaacagagg cgcataaggg
    16561 tggacctcat atcttacaaa agaccaaaga tatcgtagct agttatttag aaaaaggccc
    16621 agtagcaggt gttgccatat cttctgctgg gatggtggat ccggataagg gtgagatttt
    16681 ctatgctggg ccgcaaatcc ctaactacgc aggcacccag ttcaaaaagg aaatcgaaga
    16741 aagctttact attccttgtg agattgaaaa tgatgtcaac tgtgcaggtc ttgctgaggc
    16801 agtatctggt tcaggcaagg gagcaagtgt gacactttgc ttgaccattg gaaccggtat
    16861 cggtggttgc ttgattatgg ataggaaagt cttccatggt tttagtaatt cagcctgtga
    16921 agtcgggtat atgcatatgc aggatggagc ttttcaggat ctcgcttcta caacagcttt
    16981 agtgaaatat gtagctgaag cccatggaga agatgttgat cagtggaatg gccgacgtat
    17041 ctttaaggaa gctactgaag gaaacaaaat ctgcatggaa ggtattgacc gtatggttga
    17101 ctatctagga aaaggtctgg caaatatttg ctacgttgcc aatccagaag tggtcattct
    17161 tggtggtggt atcatggggc aagaggctat cctcaaacct aagatccgca cagccttgaa
    17221 agaggctttg gtaccaagtt tagcagaaaa aacacgatta gaatttgccc atcaccaaaa
    17281 tacagcaggg atgttgggtg catattatca ttttaagaca aaacaatcct agtttggctc
    17341 agccaaacta ggattttctt acacgttttt gtctacgata gccgttgagt tttttatttt
    17401 cccagtagct attaaagatt ttttccttgc tttcgcgatt gatttccaaa aagtaggcat
    17461 aaatcaaatc gataaagaag agcataggaa gttgagcgga tattcgttgg atataggagg
    17521 gttggctgtg ggtggctaca agaacagtct ctgtataggt ctggctattt ttattgggaa
    17581 cacttgtaaa gagtacagtc tttgccccca tctccttggc atctaataga ctatctaaaa
    17641 tagaaggagt tgagcctgaa agtgagaagc ccagtactag acaattttca tccatgatgc
    17701 tggttgtcca ggcaaagccg tcttggtctg tcaaagcttc gcagaccaca cctaatcgca
    17761 taaaacgtaa tttcatttca cgggcgacga ggccagaact ccctgttcca aagaagtaga
    17821 tacgctcagc atcttcgatt agctgggcaa ttcgttctag ttggatttcg tcaatcaagt
    17881 cttgtgtttg ttccctcata ttgctataac ttctgaggac tcgtttggtc agtggactgt
    17941 gcttggaaac ttggttggct tgattttctg cctgatgttg gtattggaaa ataaattctc
    18001 ggtagccagt aaagccacac tttttagcaa agcgggtcaa agcagcttga gaaatatgta
    18061 atttttgggt gacttgttga gaagataaat catctgtaat cgtttcagct tgcaaaaaat
    18121 agcgagcgat ttcttgctct aggtctgtca tttcttcaaa atgtgaatca atgatagttg
    18181 cgatatctgg tttgtccata gggaaagctc ctttacatga gtcatactgg aagactagat
    18241 cagggaatag tcacacttca ttataacaca taatataagg atagataaat aaaaacgcat
    18301 ctctgtttta aaaacgaaaa aatcgaaaaa gcttctctct tttccataat tttctactca
    18361 aattgtggta caattaagag taagatttta agttagaaat gagactgatt tgtatgagaa
    18421 aatttaacag ccattcgatt ccgattcggc ttaatttatt gttttcaatc gtcattttac
    18481 tctttatgac cattattggt cgtttgttgt atatgcaggt tttgaacaag gatttttacg
    18541 aaaaaaagct agcctcagct agtcagacca agattacaag cagttcagcc cgtggggaaa
    18601 tttatgatgc tagtggaaaa cctttggtag aaaatacgtt aaagcaggtt gtttccttta
    18661 cgcgtagcaa taaaatgacg gctacagact taaaagaaac agctaaaaag ttactgactt
    18721 atgtgagcat cagttctcca aatttgacag aacgccagct ggcggattac tatttggctg
    18781 atcctgaaat ctataaaaaa atagtggaag ctctcccaag cgagaaacgc ttggattcag
    18841 atggcaatcg tctatccgaa tcagaactgt ataacaatgc ggtcgatagt gtacaaacga
    18901 gtcaactaaa ctatacagag gatgaaaaga aagaaatcta tctttttagt cagttaaatg
    18961 ctgttggaaa ctttgcgaca ggaactattg ctacagatcc tctaaatgat tctcaggtgg
    19021 ctgttattgc ctctatttca aaggagatgc ctggcattag tatttctact tcttgggata
    19081 gaaaggtttt ggaaacttcc ctttcttcta tagttgggag tgtatccagt gaaaaagctg
    19141 gtctcccagc ggaagaagca gaagcctatc ttaaaaaagg ctattctcta aatgatcgtg
    19201 taggaacctc ctatttggaa aagcaatatg aagagacctt acaaggaaaa cgctcggtaa
    19261 aagaaatcca tctggataaa tatggcaata tggaaagcgt ggatacaatt gaggaaggta
    19321 gtaagggaaa caatatcaaa ctgaccattg atttggcttt ccaagatagc gtggatgctt
    19381 tactgaaaag ttatttcaat tctgagctag aaaatggtgg agccaagtat tctgaaggtg
    19441 tctatgcagt cgcccttaac ccaaaaacag gtgcggtttt gtctatgtca gggattaaac
    19501 atgacttgaa aacgggagag ttgacgcctg attccttggg aacggtaacc aatgtctttg
    19561 ttccaggttc ggttgtcaag gcggcgacca tcagctcagg ttgggaaaat ggagtcttgt
    19621 caggaaacca gaccttgaca gaccagtcca ttgtcttcca aggttcagct cccatcaatt
    19681 cttggtatac tcaggcttac ggttcattcc ctatcacagc ggtccaagct ctggagtatt
    19741 catcaaatac ctatatggtc caaacagcct taggtcttat ggggcaaacc tatcaaccca
    19801 atatgtttgt cggcaccagc aatctagagt ctgctatgga gaaactgcgt tcaacctttg
    19861 gcgaatatgg cttgggtact gcgacaggaa ttgacctacc agatgaatct actggatttg
    19921 ttcccaaaga gtatagcttt gctaattaca ttactaatgc ctttgggcag tttgataact
    19981 atacgccgat gcagttggct cagtatgtag caactattgc aaataatggt gttcgtgtgg
    20041 ctcctcgtat tgttgaaggc atttatggta ataatgataa gggaggactg ggtgacttga
    20101 ttcagcaact gcaaccgaca gagatgaata aggtcaatat atccgactcc gatatgagca
    20161 tcttgcacca aggtttttat caggttgccc atggtactag tggattgaca actggacgtg
    20221 ccttttcaaa tggtgccttg gtatccatta gcggaaaaac aggtacagcc gaaagctatg
    20281 tggcagatgg tcagcaagca accaatacca atgcggtggc ctatgcccca tctgataatc
    20341 cccaaatcgc tgtcgcagtg gtctttcctc ataataccaa tctaacaaat ggtgtaggac
    20401 cttccattgc gcgtgacatt atcaatctgt atcaaaaata ccatccaatg aactagaaag
    20461 gaaattatgc tttatccaac acctattgcc aagttgattg acagttattc taagttacca
    20521 ggtatcggga ttaagacggc tacgcgtctg gccttttata cgattgggat gtctgctgat
    20581 gatgtcaatg aatttgcaaa aaatctcctt tctgctaaga gagaattgac atattgttct
    20641 atttgtggac gtttgacaga cgacgatcct tgttctatct gtactgattc gactcgtgac
    20701 cagacaacaa ttttagttct tgaggatagt agagatgtgg cagccatgga aaatatccaa
    20761 gaataccatg gactctatca tgtcctgcat ggcctcattt ctcctatgaa tggtatcagt
    20821 ccggacgata tcaatcttaa gagccttatg actcgtctta tggatagtga ggtttcagaa
    20881 gtgattgtgg cgactaatgc tacagcggat ggtgaagcga cctccatgta tctttcacgt
    20941 ttgctcaagc cggctggtat caaggttacg cgtctagcac gaggtctcgc tgtgggagcg
    21001 gacattgagt atgcggacga agtgacactc ttacgagcga ttgaaaatcg gacagagttg
    21061 taagtgtagg caaatttacg aactccattc atttataaaa aatcaaagag gctgaaaatc
    21121 gttcctatcg gcctcttttt gtatagtgtg atgagtagaa tcaggttcaa gttttaaaaa
    21181 accaagcaaa tatgatatac taaagagcga gtattctagt agaattagga caaataatat
    21241 gaaacaaacg attattcttt tatatggtgg acggagtgcg gaacgcgaag tctctgtcct
    21301 ttcagctgag agtgtcatgc gtgcggtcaa ttacgaccgt ttcacagtca agactttctt
    21361 tatcagtcag tcaggtgact ctatcaaaac gcaggaattt agtcatgctc cggggcaaga
    21421 agaccgtctc atgaccaatg aaaccattga ttgggataag aaagttgcac caagtgctat
    21481 ctacgaagaa ggtgcagtgg tctttccagt ccttcacggg ccaatgggag aagatggctc
    21541 tgttcaagga ttcttggaag ttttgaaaat gccttacgtt ggttgcaaca ttttgtcatc
    21601 aagtcttgcc atggataaaa tcacgactaa gcgtgttctg gaatctgctg gtattgccca
    21661 agttccttat gtggctatcg ttgaaggcga tgatgtgact gctaaaatag ctgaagtgga
    21721 agaaaaattg gcttatccag tcttcactaa gccgtcaaac atggggtcta gtgtcggtat
    21781 ttctaagtct gaaaaccaag aagaactccg tcaagcctta aaacttgcct tccgatatga
    21841 cagccgtgtc ttggttgagc aaggagtgaa tgcccgtgaa attgaggttg gcctcttggg
    21901 taactacgat gtcaagagca cgctacctgg agaagttgtc aaggacgttg ccttttatga
    21961 ctacgatgcc aagtatattg ataacaaggt tactatggat attcctgcca aaatcagtga
    22021 tgatgtggtg gctgtcatgc gtcaaaatgc agaaacagcc ttccgtgcca ttggtggcct
    22081 tggtctatct cgttgcgatt tcttctatac agataaggga gagatttttc tcaacgagct
    22141 caataccatg ccaggtttca cccagtggtc tatgtaccca ctactttggg aaaatatggg
    22201 gatcagctac ccagaactaa tcgagcgttt ggttgacctt gccaaggaaa gttttgacaa
    22261 gcgcgaagcg catttgatat aaaatgaaag agagggtaga agccagaacc atcactgcaa
    22321 ggtgactaga gttctcggac ttcagccctt tttaaaggag tagaaatgaa attaacaatc
    22381 catgaaattg cccaagttgt tggagccaaa aatgatatca gtatctttga ggacactcag
    22441 ttagaaaaag ctgagtttga tagtcgtttg attggaactg gagatttatt tgtgccactt
    22501 aaaggtgcgc gtgatggcca tgactttatt gaaacagcct ttgaaaatgg tgcagcagta
    22561 accttgtctg agaaagaggt ctcaaatcat ccttacattt tagtagatga tgttttgaca
    22621 gcctttcaat ccctagcatc ctactatctt gaaaaaacga ctgttgatgt ctttgctgtt
    22681 acaggttcaa atggcaagac aacgactaag gatatgttgg cgcatttact gtcaacaaga
    22741 tacaagacct acaaaacaca aggcaattac aataatgaga ttggccttcc ttacacagtt
    22801 cttcatatgc ctgaaggaac agaaaagttg gttttggaga tgggacagga tcacttggga
    22861 gatattcatc tcttgtctga attggctcgt ccaaaaacag ccatcgtgac cttggttgga
    22921 gaagctcatt tggccttttt caaagatcgt tctgagattg ctaaaggaaa aatgcaaatt
    22981 gcagacggaa tggcttcagg ttccttgctt ttagcgccgg ctgaccctat cgtagaggac
    23041 tatttgccaa ttgataaaaa ggtggttcgt tttggtcaag gagcagagct ggaaattact
    23101 gacttggttg agcgtaagga cagtctgacc tttaaggcta atttcttgga acaagccctt
    23161 gatttgccag taactggcaa gtacaatgcg acaaatgcta tgattgcatc ctatgttgcc
    23221 ttgcaagaag gagtttcaga ggagcaaatt cgtttggcat tccaacatct tgaattgacg
    23281 cgtaaccgta ccgagtggaa gaaagcagcc aatggagcag atatcctgtc agatgtttac
    23341 aatgccaatc caactgctat gaaactgatt ttagagactt tctctgccat tccagccaat
    23401 gaaggtggca agaaaattgc agtgttggcg gatatgaagg agcttggtga ccagtctgtt
    23461 caacttcata atcagatgat tttgagcctc tccccagatg tgcttgatat cgttattttc
    23521 tatggagaag atattgctca gttggctcaa ttggccagtc aaatgtttcc aatcggccac
    23581 gtttactact tcaagaaaac agaagaccag gatcaatttg aagacctagt caagcaggtt
    23641 aaggaaagcc ttggagccta tgaccaaatc ttgctaaaag gttctaactc tatgaatcta
    23701 gccaaattgg tagaaagttt agaaaatgaa tgcaagtgat tttgctaagt atcttcaaag
    23761 aatgattgct cttacagata ctggactaac ctttacaaag gatccgtttg accgtgagcg
    23821 ctacgaagac ttgcgaagtc tgttatctga aatgttgaat caggtatcag accttgatgc
    23881 agacgaagtg gcagaactct tgaaaccgac ctccgcttat gcgactcctt taatggacgt
    23941 ccgtgcttgg atagttgagg atgaaaaaat ttgtctggtt aggggacaag gagagaatga
    24001 ttgggctttg ccaggtggct ttggcgaggt cggttattca ccaactgaaa atattctcaa
    24061 ggaaattgaa gaagaaaccg gttttaaagc caaagttgaa cgactactag ctgtttttga
    24121 taccaatcgt ttccaactac agagcaaaca atatgcaaag tttgtttttg aatgtaagct
    24181 tctcgatgga caatttcaag aaaatcaaga aatcgctgac cttcaatttt ttgcaattga
    24241 ccaactgccg aatttatctg aaaaacgcat taccaaggag caaatagagc ttctttggca
    24301 ggtttatcaa ggtcataggg ggcaatatct tgactaagaa gatgattatc gtatttctaa
    24361 atccattttt aacaactagc atggtataat aatatgcagg aaaattttga attatgagga
    24421 agactagatg aatttatggg atattttctt tacgactcag gcaaccgagc cgcccaaatt
    24481 tgaccttttt tggtatgtta gcctatttac gctcttagcc ttaacctttt atacagccca
    24541 tcgctatcgt gaaaataagg tttaccaacg atttttccaa atcttgcaga ctgttcagtt
    24601 aatccttctt tatggttggt actgggtcaa tcatatgcca ctgtcagaaa gcctaccctt
    24661 ttaccattgc cgtatggcta tgtttgtggt actcttgctt cctggtcaat ccaaatataa
    24721 acaatacttt gcattattgg gaacatttgg gacattagca gcctttgttt atccagtgcc
    24781 agatgcttac ccttttccac atatcaccat tctatccttt atctttggtc atttagcact
    24841 cttggggaac tctctagttt atctattgag acagtataat gcgcgattgc tggatgtgaa
    24901 gggaattttt ctcatgacct ttgccctaaa tgccttgatt tttgtggtca atttggtgac
    24961 aggtggcgat tacggatttt tgacaaaacc gccattggtt ggggatcacg gtctagtagc
    25021 taattattta cttgtttcaa ttgtgctggt agctactatc agtttgacta agaaaatctt
    25081 agaattcttt ttagctcaag aagcagaaaa aatgattgca aaggaagctt aacacagagc
    25141 tttctttttt gctcttagag agtttttaca agcagcttat aaaataagaa tttctgaata
    25201 gacaaactca aaaaatggct gggaaattta ggaaaaaagc aagcacgatt aaattttttg
    25261 tgttataata ttttgtgaat agctatgcct atgtttagct atggaataat acgaagtgcg
    25321 aaacttggaa gatagagagg aagcgatgta atggctagag aaggcttttt tacaggtcta
    25381 gatattggaa caagctctgt caaggtgctt gtggccgagc agagaaatgg tgaattaaat
    25441 gtaattggcg tgagtaatgc caaaagtaaa ggtgtaaagg atggaattat tgttgatatt
    25501 gatgcagcag caactgctat caagtcagcc atttcccaag cggaagaaaa ggcaggcatt
    25561 tcgattaaat cagtgaatgt cggcttgcct ggtaatcttt tgcaggtaga accaactcag
    25621 gggatgattc cagtaacatc tgatactaag gaaattacgg atcaagatgt tgaaaatgtt
    25681 gtcaaatcag ctttgacaaa gagtatgaca cctgaccgtg aagtcattac ctttattcct
    25741 gaagaattta ttgtggatgg tttccaaggg attcgtgacc cacgtggcat gatgggggtt
    25801 cgccttgaaa tgcgtggttt gctttataca ggacctcgta ctatcttgca caatttgcgt
    25861 aagacggttg agcgtgcagg tgttcaggtt gaaaatgtta tcatttcacc actagcaatg
    25921 gttcagtctg ttttgaacga aggggaacgt gaatttggtg ctacagtgat tgatatgggg
    25981 gcaggtcaaa cgactgtcgc tacaatccgt aatcaagaac tccagttcac acatattctc
    26041 caagaaggtg gagattatgt aactaaagat atctccaagg ttttgaaaac ctctcgcaaa
    26101 ttagcggaag gcttaaaact gaattacggg gaagcctatc cgcctcttgc aagcaaagaa
    26161 accttccaag tagaggttat tggagaagta gaagcagtcg aagtgacgga agcctacttg
    26221 tcagaaatta tttctgcacg aatcaagcac atccttgaac aaatcaagca agaattagat
    26281 agaaggcgtc tattggacct ccctggtggt attgtcttaa tcggtgggaa tgccatttta
    26341 ccaggtatgg ttgagcttgc tcaggaagtc tttggcgtcc gtgtcaagct ttatgttcca
    26401 aatcaagttg gtatccgtaa tccagccttt gcgcatgtga ttagtttatc agaatttgcg
    26461 ggtcaattaa cagaagttaa tcttttggct cagggagcga taaaaggtga gaatgactta
    26521 agtcatcagc caattagttt tggtgggatg ctgcaaaaaa cagctcagtt tgtacaatca
    26581 acgcctgttc aaccagctcc tgctccagaa gtagagccgg tggcgcctac agaaccaatg
    26641 gcggatttcc aacaagcttc acaaaataaa ccgaaattag cagatcgttt ccgtggcttg
    26701 atcggaagca tgtttgacga ataaagagga aaaataaatt atgacatttt catttgatac
    26761 agctgctgct caaggggcag tgattaaagt aattggtgtc ggtggaggtg gtggcaatgc
    26821 catcaaccgt atggtcgacg aaggtgttac aggcgtagaa tttatcgcag caaacacaga
    26881 tgtacaagca ttgagtagta caaaagctga gactgttatt cagttgggac ctaaattgac
    26941 tcgtggtttg ggtgcaggag gtcaacctga ggttggtcgt aaagccgctg aagaaagcga
    27001 agaaacactg acggaagcta ttagtggtgc cgatatggtc ttcatcactg ctggtatggg
    27061 aggaggctct ggaactggag ctgctcctgt tattgctcgt atcgccaaag atttaggtgc
    27121 gcttacagtt ggtgttgtaa cacgtccctt tggttttgaa ggaagtaagc gtggacaatt
    27181 tgctgtagaa ggaatcaatc aacttcgtga gcatgtagac actctattga ttatctcaaa
    27241 caacaatttg cttgaaattg ttgataagaa aacaccgctt ttggaggctc ttagcgaagc
    27301 ggataacgtt cttcgtcaag gtgttcaagg gattaccgat ttgattacca atccaggatt
    27361 gattaacctt gactttgccg atgtgaaaac ggtaatggca aacaaaggga atgctcttat
    27421 gggtattggt atcggtagtg gagaagaacg tgtggtagaa gcggcacgta aggcaatcta
    27481 ttcaccactt cttgaaacaa ctattgacgg tgctgaggat gttatcgtca acgttactgg
    27541 tggtcttgac ttaaccttga ttgaggcaga agaggcttca caaattgtga accaggcagc
    27601 aggtcaagga gtgaacatct ggctcggtac ttcaattgat gaaagtatgc gtgatgaaat
    27661 tcgtgtaaca gttgtcgcaa cgggtgttcg tcaagaccgc gtagaaaagg ttgtggctcc
    27721 acaagctaga tctgctacta actaccgtga gacagtgaaa ccagctcatt cacatggctt
    27781 tgatcgtcat tttgatatgg cagaaacagt tgaattgcca aaacaaaatc cacgtcgttt
    27841 ggaaccaact caggcatctg cttttggtga ttgggatctt cgccgtgaat cgattgttcg
    27901 tacaacagat tcagtcgttt ctccagtcga gcgctttgaa gccccaattt cacaagatga
    27961 agatgaattg gatacacctc catttttcaa aaatcgttaa gtaaatgaat gtaaaagaaa
    28021 atacagaact tgtttttcga gaagttgcag aggctagtct gagtgctcat cgagagagtg
    28081 gttcggtctc tgtcattgca gttaccaagt atgtagatgt accgacagcg gaagccttgc
    28141 ttccgctagg tgttcatcat atcggtgaaa atcgtgtaga taagtttctg gaaaaatatg
    28201 aagctttaaa agatcgagat gtgacttggc atttgattgg taccttgcaa agacgtaagg
    28261 tgaaagatgt cattcaatac gttgattatt tccatgcatt ggactcagta aagctagcag
    28321 gggaaattca aaaaagaagt gaccgagtca tcaagtgttt ccttcaagta aatatttcta
    28381 aagaagaaag caaacacggt ttttcgagag aggaactgct ggaaatcttg ccagagttag
    28441 ccagactaga taagattgaa tatgttggtt taatgacgat ggcacctttt gaggctagca
    28501 gtgagcagtt gaaagagatt ttcaaggcgg cccaagattt acaaagagaa attcaagaga
    28561 aacaaattcc aaatatgcct atgaccgagt taagtatggg aatgagtcgt gattataaag
    28621 aagcgattca attcggttcc acttttgttc gtataggtac atcatttttt aagtaggaga
    28681 gaaccatgtc tttaaaagat agattcgata gatttataga ttattttacg gaggatgagg
    28741 attcaagtct cccttatgaa aaaagagatg agcctgtgtt tactccagta aattcttcac
    28801 aggaaccggc tctcccaatg aatcaacctt cacagtcggc tggtacaaaa gagaacaata
    28861 tcaccagact tcatgcaaga caacaggaat tggcaaatca gagtcagcgt gcaacggata
    28921 aggtcattat agatgttcgt tatcctagaa aatatgagga tgcaacagaa attgttgatt
    28981 tattggcagg aaacgaaagt atcttgattg attttcagta tatgacagag gtgcaggctc
    29041 gtcgttgttt ggactatttg gatggagctt gtcatgtttt agctggaaat ttgaaaaagg
    29101 tagcttctac catgtatttg ttgacaccag ttaatgttat tgtaaatgtt gaagatatcc
    29161 gtttaccaga tgaagatcaa cagggtgagt tcggttttga tatgaagcga aatagagtac
    29221 gataatgatt tttttaattc gtatgattta taatgcagtg gatatttact ccctgatttt
    29281 ggtagccttc gctgtcatgt cttggtttcc aggtgcctac gaatccagtt taggtcgttg
    29341 gattgtagcg ttggtgaaac cagtacttgc tcccttgcaa cgcctgcctt tacagatagc
    29401 gggtcttgat ttatctgttt gggtcgcgat tgttttggtt cgatttttag gagaaaacct
    29461 agtgcgtttt ctggcgatga taggatgaat aaagggattt atcagcattt ctccatagaa
    29521 gatcgtccat ttcttgacaa gggaatggaa tggataaaga aggtagaaga tagctatgct
    29581 ccttttttaa ctccttttat caatcctcat caggagaagc tattaaagat tttggccaaa
    29641 acctatggtc ttgcttgtag cagtagtggg gaattcgtct cgagtgagta tgttcgagtt
    29701 ttattatacc cagattattt ccaaccagag ttttcagatt ttgaaatatc tctccaagaa
    29761 attgtgtatt ccaataaatt tgaacattta acgcatgcta agattttagg gacagtcatc
    29821 aatcaattag ggattgaacg gaaacttttt ggagatatcc tagtagatga agaacgggcg
    29881 cagattatga ttaatcagca gtttcttctt ctctttcaag atggactaaa gaaaattggt
    29941 cgtatacctg tttcgctgga ggaacgtcct ttcaccgaga aaatagataa gctagaacag
    30001 tatcgagaac tggatttatc tgtgtctagt tttcgattag atgttctttt atcaaatgtt
    30061 ttgaaactat ctaggaatca agcaaaccag ttgattgaaa agaaacttgt ccaagtaaat
    30121 tatcatgtgg tagacaaatc agattacact gttcaagttg gagacttgat tagtgtgaga
    30181 aaatttggtc gcttgagatt acttcaagat aagggacaaa cgaaaaaaga gaagaaaaaa
    30241 ataaccgtcc agttattatt aagtaagtga ggaatagaat gccaattaca tcattagaaa
    30301 taaaggacaa gacttttgga actcgattca gaggttttga tccagaagaa gtcgatgaat
    30361 ttttagatat tgtggttcgt gattacgaag atcttgtgcg tgcgaatcat gataaaaatt
    30421 tgcgtattaa gagtttagaa gagcgtttgt cttactttga tgaaataaaa gattcattga
    30481 gccagtctgt attgattgct caggatacag ctgagagagt gaaacaggcg gcgcatgaac
    30541 gttcaaacaa tatcattcat caagcagagc aagatgcgca acgcttgttg gaagaagcta
    30601 aatataaggc aaacgagatt cttcgtcaag caactgataa tgctaagaaa gtcgctgttg
    30661 aaacagaaga attgaagaac aagagccgtg tcttccacca acgtctcaaa tctacaattg
    30721 agagtcagtt ggctattgtt gaatcttcag attgggaaga tattctccgt ccaacagcta
    30781 cttatcttca aactagtgat gaagccttta aagaagtggt tagcgaagta cttggagaac
    30841 cgattccagc tccaattgaa gaagaaccaa ttgatatgac acgtcagttc tctcaagcag
    30901 aaatggaaga attacaagct cgtattgagg tagccgataa agaattgtct gaatttgaag
    30961 ctcagattaa acaggaagtg gaaactccaa ctcctgtagt gagtcctcaa gttgaagaag
    31021 agcctctgct catccagttg gcccaatgta tgaagaacca gaagtagctc cagtgcatcc
    31081 gacaggtcca acaccagcta cagaaactgt tgattcagca ccaggatttg aagcaccgca
    31141 agaatccgtt acaattttat aagaaacgat gtgagaacaa tatcttatcc ttatatttcc
    31201 agcgagcagg agatggtgtg agtcctgcat tccctaacga taagattatc ctctcaaaaa
    31261 ctcaagtctg aagctagtaa gatttgacgt ttcccacgtt acgggataag agggagaaag
    31321 actaaatctt tttccgaata aaggtggtac cacgattttc gtcctttttg gaagtcgtgg
    31381 tttttaattt gttattattt ataaaggaga taccatgaaa ctcaaagata cccttaatct
    31441 tgggaaaaca gctttcccaa tgcgtgcagg ccttccgacc aaagagccag tttggcaaaa
    31501 ggaatgggaa gatgcaaaac tttaccaacg tcgtcaagaa ctaaatgaag ggaaaccgca
    31561 ttttgtcttg catgatggcc ctccatacgc aaacggaaat atccacgttg gacatgccat
    31621 gaatcatatc tcaaaagata tcatcattcg ttctaagtct atgtcaggtt ttaacgcacc
    31681 ttatatccca ggttgggata ctcatggtct gccaatcgag caagttttgg caaagcaagg
    31741 tgtgaagcgc aaagaaatgg acttggttga gtacttgaaa ctctgtcgtg agtacgctct
    31801 ttctcaagtt tataaacaac gtgatgattt caaacgcttg gggatgtcag gtgactggga
    31861 aaatttatat gtgactttaa ctccagacta tgaagcagcc caaatccgtg tctttggtga
    31921 gatggctaat aagggttata tctaccgtgg tgccaagcca gtttactggt catggtcatc
    31981 tgagtcagcc cttgccgaag cggaaattga ataccatgat ttggtttcga cttcacttta
    32041 ttatgccaat aaggtaaaag atggcaaagg tatcctagat acagatactt atatcgtagt
    32101 ctggacaaca actccattta ccatcacagc ttctcgtggt ttgactgttg gtgcagatat
    32161 tgattacgtt ttggttcaac ctgctagtga aactcgtaag tttgtcgttg ctgcagaatt
    32221 gttgactagc ttgtctgaga aatttggctg ggctgatgtt caagtgttgg caacttaccg
    32281 tggtcaagaa ctcaaccaca tcgtaacaga acacccatgg gatacagctg tagatgagtt
    32341 ggtaatcctt ggtgatcacg ttaccactga ctctggtaca ggtatcgtcc atacagcccc
    32401 tggttttggt gaggacgact acaatgttgg tattgctaat ggtcttgaag tcgcagtgac
    32461 tgttgacgaa cgcggtatca tgatggcgaa tgctggtcct gagtttgaag gtcagttcta
    32521 cgacaaggtt gtaccaactg ttattgaaaa acttggtaac ctccttctag cccaagaaga
    32581 aatctctcac tcatacccat ttgactggcg tactaagaaa ccaatcatct ggcgtgcagt
    32641 tccacaatgg tttgcctcag tttcaaaatt ccgccaagaa atcttggatg ctattgataa
    32701 agttaaattc catacagaat ggggtaaagt acgtctatat aatatgattc gtgaccgtgg
    32761 tgactgggtt atctctcgtc aacgtgcttg gggtgttcca cttccaatct tctatgcaga
    32821 agacggtaca gctatcatga cagctgagac tattgaacat gtggcgcaac tctttgaagt
    32881 acatggttca agcatttggt gggaacgtga tgccaaagac ctcttaccag aaggatttac
    32941 acatccaggt tctccaaatg gggagttcaa gaaagaaaca gatattatgg atgtatggtt
    33001 tgactcaggt tcatcatgga atggtgtttt ggtaaatcgt ccaaatctga cttatccagc
    33061 tgacctttac cttgaaggtt ctgaccaata ccgtggttgg ttcaactcat cactcattac
    33121 atctgttgcc aaccatggcg tagcacctta caaacaaatc ttgtcacaag gttttaccct
    33181 tgatggtaaa ggtgagaaga tgtctaaatc acttggaaat accattgctc caagtgatgt
    33241 tgaaaaacaa tttggtgcag aaatcttgcg tctctgggta acaagtgttg actcaagcaa
    33301 tgacgtgcgt atctctatgg atattttgag ccaagtttct gaaacttacc gtaagattcg
    33361 taacactctt cgtttcttga ttgccaatac atctgacttt aacccagctc aagatgtagt
    33421 cgcttacgat gaacttcgtt cagttgataa gtacatgact attcgcttta accagcttgt
    33481 caagaccatt cgtgatgcct atgcggactt tgaattcttg acaatctaca aggccttggt
    33541 gaactttatc aacgttgact tgtcagcctt ctaccttgat tttgccaaag atgttgttta
    33601 cattgaaggt gccaaatcat tggaacgccg tcaaatgcag actgttttct atgacattct
    33661 tgtcaaaatc accaaactct tgacaccaat ccttcctcac actgcggaag aaatctggtc
    33721 atatcttgag tttgaagctg aagacttcgt tcaattgtca gaattgccag aagctcaaac
    33781 ttttgctaat caagaagaag tcttggatac atgggcagcc ttcatggact tccgtggaca
    33841 agctcaaaaa gccttggaag aagctcgtaa tgcaaaagtt atcgggaaat cgcttgaagc
    33901 acacttgaca gtttatccaa atgaagttgt gaaaactcta ctcgaagcag taaacagcaa
    33961 tgtggctcaa cttttgatcg tgtcagactt gaccatcgca gaagggccag ctccagaagc
    34021 tgcccttagc ttcgaagatg tagccttcac agttgaacgc gctgcaggtc aagtatgtga
    34081 ccgttgccgt cgtatcgacc caacaacagc agaacgcagc taccaggcag tcatctgtga
    34141 ccactgtgca agcatcgtag aagaaaactt tgcggaagca gtcgcagaag gatttgaaga
    34201 gaaataaggt tgaaaagtct aggcaaaatt caatttgaga agaaaagaca actaatttta
    34261 tagtctatta aacgcattgt atcacgtttt tgaatacctg atatgatgcg ttttttattt
    34321 attttaaaaa tttgcgaggt atgacttttt atactcaaca agaatcaaag agaaacttag
    34381 caagctaaca gtagtaagat aaaataggaa tttgata
//
LOCUS       DAVLVF010000017        33884 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVLVF010000017 DAVLVF010000000
VERSION     DAVLVF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.017"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..4071
                     /locus_tag="VYN66_000747"
     CDS             <1..4071
                     /locus_tag="VYN66_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1494808.1"
                     /translation="KGEKGDQGERGLTGAQGAKGEKGDQGEQGLQGRDGEQGPKGEDG
                     KNPTVKVTDGQDGTHTITINDGKGGITTTVVRDGFDGASPLVSTHRNEADKTTTVIFY
                     YDLNDNNQFDEGDTKLKEVVIADGKQGPKGDKGDNGKDGFTPEVTVTDNNNGTHTITI
                     TQPDNRPSLTTIVKNGEDGKTPKVKAERDDAKKQTTLTFYIDKDGDGSYTAGKDELVQ
                     TTVVKDGQDGAAGASGRDGKEVLNGKVDPTTEGKDGDTFVNTQTGDVFVKKGNTWEPA
                     GNIKGPKGDKGADGAKGEKGAQGERGLTGAQGAKGADGAAGRDGRDGRDGKDVLNGKV
                     NPEENQGKDGDKYVNTETGDVYVKNNGNWDKEGNIKGPKGDKGADGAKGEKGDRGERG
                     LTGAQGAKGADGAAGRDGRDGRDGKDVLNGKVNPEANQGKDGDKYVNTETGDVFVKNN
                     GNWDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDGHSTDITFTVPGKDPVTVNVKDG
                     ENGLNGKTPKVDLLRVQGQNGNPPHTIVTFYTDENNDGKYTPGTDELLGSEMIKDGAK
                     GADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPLAVIRDGVDGKSPTVTAVRK
                     DEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGATGQDGQTPTITTQRGQDGQSTVV
                     TITTSGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRIRRDTDAAATPTRNGIRVTV
                     YDDVNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPTITTKDNGDGTHTITVQNPDGSE
                     STTVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKETVVKNGKDGKTPKVEVTDN
                     NDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPDGSHTVTITNPDGTKNEFVV
                     KNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETTVRDGKSPKVTITDEQNGTH
                     KISVLNGDGTTTETIIKDGKSPVATVRDNQDGTYTIRVENGNGTVSETTVRDGKSPTA
                     KVVDNGDGTHTITVVNSDGTTTTTTVRDGREPKLEVIDNNDGSHTIKVTGADGKGTTT
                     TIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDSVSPTVTVKNNNDG
                     THVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHTITIINSDGTVTKTVIKDG
                     KDGRDGRDGRDGKDGKDGKDGKCGCQDKPVTPSNDKPVPPTPNVPTPEVPVKPVPAQP
                     TPNVPTPEVPVQPTPAVPTPEVPVKPVPAVPEQPVVPTPAQPATPVNANPVAPTTGKE
                     NRGDKLPETGSQSDYISVLLGSGILLSLYVGRRKED"
     gene            complement(4308..4856)
                     /locus_tag="VYN66_000748"
     CDS             complement(4308..4856)
                     /locus_tag="VYN66_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1494809.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            5177..5665
                     /locus_tag="VYN66_000749"
     CDS             5177..5665
                     /locus_tag="VYN66_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV1494810.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISGSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQVDQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            5662..6849
                     /locus_tag="VYN66_000750"
     CDS             5662..6849
                     /locus_tag="VYN66_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1494811.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSTKEDNHFKVTVDQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            6842..7207
                     /locus_tag="VYN66_000751"
     CDS             6842..7207
                     /locus_tag="VYN66_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1494812.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            7223..8569
                     /gene="asnS"
                     /locus_tag="VYN66_000752"
     CDS             7223..8569
                     /gene="asnS"
                     /locus_tag="VYN66_000752"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV1494813.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            8722..9012
                     /locus_tag="VYN66_000753"
     CDS             8722..9012
                     /locus_tag="VYN66_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV1494814.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            9024..9494
                     /gene="ssbA"
                     /locus_tag="VYN66_000754"
     CDS             9024..9494
                     /gene="ssbA"
                     /locus_tag="VYN66_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV1494815.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            9526..9765
                     /locus_tag="VYN66_000755"
     CDS             9526..9765
                     /locus_tag="VYN66_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV1494816.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            10060..11460
                     /locus_tag="VYN66_000756"
     CDS             10060..11460
                     /locus_tag="VYN66_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV1494817.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGVFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            11462..11821
                     /locus_tag="VYN66_000757"
     CDS             11462..11821
                     /locus_tag="VYN66_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV1494818.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            11875..12624
                     /locus_tag="VYN66_000758"
     CDS             11875..12624
                     /locus_tag="VYN66_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV1494819.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVNPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            12614..12913
                     /locus_tag="VYN66_000759"
     CDS             12614..12913
                     /locus_tag="VYN66_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1494820.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            12984..13919
                     /locus_tag="VYN66_000760"
     CDS             12984..13919
                     /locus_tag="VYN66_000760"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV1494821.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            14005..14517
                     /locus_tag="VYN66_000761"
     CDS             14005..14517
                     /locus_tag="VYN66_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV1494822.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAPGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            14542..15150
                     /locus_tag="VYN66_000762"
     CDS             14542..15150
                     /locus_tag="VYN66_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEV1494823.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            15251..15448
                     /locus_tag="VYN66_000763"
     CDS             15251..15448
                     /locus_tag="VYN66_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1494824.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(15491..16936)
                     /locus_tag="VYN66_000764"
     CDS             complement(15491..16936)
                     /locus_tag="VYN66_000764"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV1494825.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVRRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVKDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            17042..18664
                     /locus_tag="VYN66_000765"
     CDS             17042..18664
                     /locus_tag="VYN66_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1494826.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLCSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            18868..20826
                     /locus_tag="VYN66_000766"
     CDS             18868..20826
                     /locus_tag="VYN66_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1494827.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSVQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            20998..21231
                     /locus_tag="VYN66_000767"
     CDS             20998..21231
                     /locus_tag="VYN66_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEV1494828.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKR"
     gene            21237..22679
                     /locus_tag="VYN66_000768"
     CDS             21237..22679
                     /locus_tag="VYN66_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494829.1"
                     /translation="MRKKVIEGLIYAEKSFFDKSQSGELTSAIVNDMSVIREFLITTF
                     PNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISEKYSRRLQEEIG
                     FLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSFETPFALLFI
                     FATIVVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTIYSSSKGSSVA
                     LENLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKGQKIAFVGPS
                     GSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDN
                     LCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGRSQRLQIARA
                     YLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFFLE
                     EGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            22837..23931
                     /locus_tag="VYN66_000769"
     CDS             22837..23931
                     /locus_tag="VYN66_000769"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1494830.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            23939..25105
                     /locus_tag="VYN66_000770"
     CDS             23939..25105
                     /locus_tag="VYN66_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1494831.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IHQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            25198..28296
                     /locus_tag="VYN66_000771"
     CDS             25198..28296
                     /locus_tag="VYN66_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1494832.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLIFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVDRQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAIQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            28344..28961
                     /locus_tag="VYN66_000772"
     CDS             28344..28961
                     /locus_tag="VYN66_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1494833.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            28972..30306
                     /locus_tag="VYN66_000773"
     CDS             28972..30306
                     /locus_tag="VYN66_000773"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV1494834.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLYQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            30316..30780
                     /locus_tag="VYN66_000774"
     CDS             30316..30780
                     /locus_tag="VYN66_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1494835.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            30777..31271
                     /locus_tag="VYN66_000775"
     CDS             30777..31271
                     /locus_tag="VYN66_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1494836.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            31352..33007
                     /gene="mltG"
                     /locus_tag="VYN66_000776"
     CDS             31352..33007
                     /gene="mltG"
                     /locus_tag="VYN66_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV1494837.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            33078..33560
                     /gene="greA"
                     /locus_tag="VYN66_000777"
     CDS             33078..33560
                     /gene="greA"
                     /locus_tag="VYN66_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV1494838.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(33617..>33884)
                     /locus_tag="VYN66_000778"
     CDS             complement(33617..>33884)
                     /locus_tag="VYN66_000778"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494839.1"
                     /translation="EVVDRTDEVSSKHGFEVVDETDEVSSKHGFEVVDETDEVSSKHG
                     FEVVDETDEVGSKHGFEVVDETDEVSNHTYGKATLTWFEEIFEE"
BASE COUNT        10507 a         6312 c         7644 g         9421 t
ORIGIN      
        1 aaaggtgaaa aaggggacca aggtgaacgc ggtctaaccg gagcacaagg tgctaaaggt
       61 gaaaaagggg accaaggtga acaaggtctt caagggcgtg atggtgaaca aggaccaaaa
      121 ggagaagatg ggaaaaaccc aacagttaaa gtaactgatg gtcaagatgg aacgcataca
      181 attacaatta acgatggtaa aggtggtata actactacag tagtaagaga tggatttgat
      241 ggtgcaagcc ctcttgtttc tactcataga aatgaagcag ataaaacaac aactgttatt
      301 ttctattatg atctaaatga taataatcaa tttgatgaag gagatacaaa acttaaagaa
      361 gttgttatcg cagatggaaa acaaggacca aaaggtgaca aaggtgataa cggaaaagat
      421 ggattcacac cagaagtaac agttacagat aacaataacg gaacacacac catcacaatc
      481 acacaaccag acaacagacc atcattaaca acaatcgtta aaaacggtga agatggaaaa
      541 acaccaaaag tcaaagcaga acgagatgat gcgaagaaac aaacaacatt aacattctac
      601 attgacaaag atggagatgg aagttacaca gcaggaaaag acgagttagt tcaaacaaca
      661 gtagttaaag acggacaaga cggagctgca ggagcatctg gacgtgatgg aaaagaagta
      721 ttaaacggaa aagtagaccc aacaacagaa ggtaaagatg gagacacatt cgtaaataca
      781 caaacaggag atgtattcgt taagaaaggt aacacttggg aaccagcagg aaacatcaaa
      841 ggaccgaaag gtgacaaagg tgcagatggt gccaagggtg aaaaaggagc ccaaggagaa
      901 cgcggcctga ctggagcgca aggagctaaa ggtgcggatg gagcagcagg tcgtgacgga
      961 cgtgatggac gtgacggtaa agacgtgttg aacggcaaag ttaacccaga agaaaatcaa
     1021 ggtaaagacg gcgataaata cgttaataca gaaacaggtg atgtctatgt taaaaacaat
     1081 ggaaactggg ataaagaggg caacatcaaa ggacctaaag gtgacaaagg tgcagatggt
     1141 gccaaaggcg aaaaaggaga ccgaggcgaa cgcggcctga ctggagcgca aggagctaaa
     1201 ggtgcggatg gagcagcagg tcgtgacgga cgtgatggac gtgacggtaa agacgtgttg
     1261 aacggcaaag ttaacccaga agcaaatcaa ggtaaagacg gtgataaata cgtcaataca
     1321 gaaacaggcg acgtcttcgt taagaataac ggcaactggg ataaagaggg caacatcaag
     1381 ggacctaaag gggacaaagg tgaacgcgga gaagatggta agactccaga agtaactgta
     1441 actccaggta aagatggcca tagtactgac attacattca ctgttccagg taaagatcca
     1501 gttacagtaa atgttaagga cggagaaaat ggtctgaacg gtaaaactcc aaaagttgat
     1561 ttacttcgtg tccaaggaca aaacggaaat ccacctcata caattgtgac attctataca
     1621 gatgaaaaca atgatggcaa atatacacca ggaactgatg aacttctagg ttcagaaatg
     1681 attaaagatg gtgctaaagg tgcggacgga cgagatggta aatcattgct tactgtcaag
     1741 gatggtaaag aaactaaagt ttaccaagaa gatccagcta acccaggaca accattaaat
     1801 ccagaaaaac cacttgcggt aattagagat ggagtagatg gaaaatcacc tacagttaca
     1861 gctgttcgta aagatgaagc agggcataaa ggtgtagaaa tcactgttga taaccatgat
     1921 ggttcacaac caactacagt ctttgttcaa gatggtgcta agggagaaac tggtgcaacc
     1981 ggtcaagatg gacaaactcc tacaatcact actcaacgtg gacaagatgg ccaaagcact
     2041 gttgtaacta tcacaacatc aggtaaagat ccagtaacct tcactgtaaa agatggtaag
     2101 aatggtaaag atggccgtgc accgaaaatc aaagtagaag atattacttc accttcaaga
     2161 attagacgcg atacagatgc tgctgcaact ccaacgcgta acggtatccg tgttacagtt
     2221 tatgatgatg ttaatgacaa tggggtatac gacgaaggtg tcgataaagt attaaatagt
     2281 aaagatattt ataacggtat agatggacgt gatggttcag ctccaactat tactacaaaa
     2341 gataatggag atggaactca cactatcaca gttcaaaacc cagatggttc tgaatcaaca
     2401 acagttgtta aagatggtaa agacggtaaa actgcgaata tcactacaac agaaaaccca
     2461 gatggaagcc acacaattac ggtgacaaat ccagatggtt caactaaaga aactgttgtt
     2521 aaaaacggta aagacggtaa gactcctaaa gttgaagtaa cggataacaa cgatggaact
     2581 catactgtta aagttacaga tggagacggc aatgttacca acgctatcat caaagatggt
     2641 aaagacggta aagctgcaac agcaacaact actgaaaatc cagatggaag ccacacagta
     2701 acaatcacta acccagacgg aactaagaat gagtttgttg ttaagaatgg acgtgacggt
     2761 gttgacggac gtactccaac cgcatctgtt cgtgataatg gagacggaag tcatacaatc
     2821 gttattacaa atccagaagg tgtgacaact gaaaccacag ttcgtgatgg taaatcacca
     2881 aaagtgacta taactgatga acaaaatgga actcataaga tctctgttct aaatggtgac
     2941 ggaacaacta ctgaaacaat cattaaagat ggtaaatcac cagtagcaac agttagagat
     3001 aaccaagatg gtacttacac tattcgtgtg gaaaacggta atggtactgt ttctgaaacc
     3061 acagttcgtg acggtaaatc accaactgct aaggttgtgg ataatggaga tggaactcac
     3121 actatcacag ttgtgaactc agacggaaca actacaacaa ctacagttcg tgatggtaga
     3181 gaaccaaaac ttgaagttat tgataacaac gatggttcac acactattaa agtgacaggt
     3241 gctgatggta aaggaacgac aactacaatc tttgatggta aatcaccaaa agcgaacatc
     3301 gttgataacg gagatggaac tcatacatta acaatcgtag attctgatgg tcgtgaatac
     3361 aaatctatta tcaaagatgg taaagacggc aaagatagcg tttcaccaac tgtaactgtt
     3421 aaaaataata acgatggaac tcacgttgtt acaatcacta atccagatgg aagtaagaca
     3481 gaaatggtga ttaaagacgg taaagatggt aaatcaccaa aagtttctgt tgaagataat
     3541 ggtgatggta gtcatacaat cacaatcatc aattctgatg gaactgtgac aaaaacagtt
     3601 attaaagatg gcaaagatgg tagagatgga cgtgatggtc gagacggcaa agacggtaaa
     3661 gacggtaaag atggaaaatg tggatgccaa gacaaaccag taacaccatc aaatgacaaa
     3721 ccagttcctc caacaccaaa tgtgccgaca ccagaagtac cggttaaacc tgtgccagcg
     3781 caaccaacac caaatgtacc gacaccagaa gtgccagtac aaccaactcc agctgttcca
     3841 acaccagaag taccggttaa accagtacca gcggttccag aacaaccagt agtaccaaca
     3901 ccggctcaac cagcaactcc agtaaatgct aatccagtag caccaactac aggtaaagaa
     3961 aaccgtgggg acaaattacc tgaaactgga agccaatctg attatatctc tgttctttta
     4021 ggtagcggta ttctattgag cctatatgta ggacgaagaa aagaagatta atcagccaaa
     4081 agcagtcact aatttctcga aagaagatga taagtgatag atctcgaatg aaagaatatt
     4141 cttagaatga ttttcctatg atggaaaaga ctgctaaaaa ccttgagtgg taacgctcaa
     4201 ggtttttttg gatggctgat gtttgtctga aaatcttgat tgagaaataa aaagccaccc
     4261 tattaggtgg ctataattga tttagacttt ctaaaatttt ctatttttta ctgaatcgct
     4321 tcgaccaagt cttgagcttg tttttcaagt gagtttagga ctgtttcttc aagaaccaat
     4381 tttccgtctg cccaggcaga gtcattaaca cgtgcagcag tgaaatcacc aacgccttgt
     4441 gtacggataa atggcaagag gtctttgtag atagcgaaaa gttgatcgtg ccctgcattg
     4501 gctacagatg atactgtgac aaacttgtct tgaagggcag aaacgccacg tgtatcagac
     4561 aagtcaaggg cacgagatag ccagtcaagc aagtttttca ctgtaccagg gatagagaag
     4621 ttgtagactg gagagaaaat ccagatagca tccgcaacga gaactgcttc acgagcagca
     4681 gctacagctg gatgtgttgg aacttccaaa tcttggctga agagaggaag ggctgaataa
     4741 tcaaggtagc taacttccgc tttaccagca agtgctttct cagcttcgag cgccatttgg
     4801 tggttgaaag aaccttgacg tagtgatccg acgataaata atactttttt agacatgatg
     4861 tgtctccttt ttcttaaaat ttcgagaact ctctcgttgt tataaaatat attacaacaa
     4921 aacaaaagaa cttgcaagaa atttgctcag agtttatttg aaaattgtaa aatttcggaa
     4981 actctaatca tttgtgactg aaaaagtaag aactgtttca ctttcaaaat cggttttgtt
     5041 tgaagggaga aatagaattg gttgctcaaa gcacagcttt gaggttgcag ataaagctga
     5101 cgtggtttga agagattttc gaagagtatt cttatattca cttgtaataa agcttaatag
     5161 atggtaaaat agacgagtga aaaaaagaca aaacaaagca aaaaataatc tactgtggca
     5221 gtatggtcta gggatgacga ttttgtttgt ggttatcagt ggttcctttc tgtatatggt
     5281 ttctcttagc atgaaaccct atcaaacagc taaaagtgaa ggagaaaaat tagctcagca
     5341 gtatgcagga ttagagcagg tcgatcaggt tgatttatac aatggcttgg aatcttatta
     5401 cagcgttctt ggtcgtaata aacagcaaga agcgcttgct gtcctgattg gtaaagatga
     5461 ccataagatt tacgtttatc agctaaatca gggtgtttca caagaaaaag cagaaacggt
     5521 ttctaaggaa aagggagctg gcgagattga caagataacc tttggccgtt atcaagataa
     5581 gccaatctgg gaagttaagt caggatctga tttttatcta gtagattttg aaacaggagc
     5641 attggtcaac aaggagggcc tatgaaacta tccaaccgtg ttttagaaat ggaagaaagt
     5701 gtgactttgg ctgctggagc ccgtgccaaa gcactgaagg ctgagggtag agatattttg
     5761 tctctaacct tgggtgagcc agatttcact actcccaaaa atatccaaga tgccgccatt
     5821 gcatcgattc gagatgggcg tgcttctttt tatacagtaa cctcaggtct gccagagctt
     5881 aaggcggcgg tcaatagcta ctttgagcgc ttttacggct attctgtagc gtcaaatcaa
     5941 gtgacagtcg ctgcgggagc caaatattct ctctatacct tctttatggc tgtggtcaat
     6001 ccaggtgatg aagtcatcat cccaacccca tactgggtta gctatggaga tcaggtcaag
     6061 atggcagaag gcgttcccgt ctttgtttct actaaggaag acaatcactt taaggtgacc
     6121 gtagatcagt tagaagcagc tcgcactgac aagaccaagg ttttggtgct gaattcgcca
     6181 tctaatccca caggtatgat ttacacccgt gaggaactct tggcaattgg aaactgggct
     6241 gtagaaaatg atattctcat cctagcagat gatatctatg gccgcttggt ttataatggt
     6301 catgagttca caccgatttc tagcctatcg gaagcgattc gcaagcaaac agtggtcatc
     6361 aatggtgtgt ctaaaaccta tgccatgact ggttggcgga ttggttatgc cgttggagaa
     6421 gcagatatta tcgctgccat gtccaagatt gcaggtcaaa caacttcgaa tccgtcagca
     6481 gtagcccaat atgcagcagt tgaggctcta tcaggtgagc aagatactgt agaaagcatg
     6541 cgtcaggcct ttgaggaacg tcttaatacc atctatcccc tccttgcaga ggtaccagga
     6601 tttgaagtgg tcaaaccgca aggggccttc tatctcttcc caaatgtcaa aaaggccatg
     6661 gagatgaaag gctacacgga tgtgacagac tttacaactg ctatcttaga agaagccgaa
     6721 gtggccttgg taacaggagc tggatttgga gcaccagaaa atgtccgcct cagctatgcg
     6781 acagacctag acacgcttaa agaagcagtc gaacgcttga aagcatttat gggtagtgag
     6841 aatgattgat cattttgaga ttaaggtaaa ggatttacaa atttcagaag gattttatag
     6901 gagttttctc gctcctttgg actataaatt ggcttttaag actagttctc taattagttt
     6961 tctatccccg aacagccctc atcctggtgg tgatttttgg ctgactcagg gaacacaaga
     7021 tcctgttcat tttgcttttt tagcagaaaa taaggaagaa gttcaggctt gttatgaggc
     7081 tggcttagag gcaggtgggc gagacaatgg ggctcctggt tatcgaagtg agcatccgat
     7141 ttactatgct gcttttatga ttgacctgga tgggaacaat atagaggtgg tttgccataa
     7201 agaataaaaa agaaaaggaa aaataatgac aaaacgtgta acgattattg acgtaaaaga
     7261 ctatgttggt caggaagtga cgattggcgc ttgggttgcc aacaaatcag gaaaaggaaa
     7321 aatcgctttc ttacaattgc gtgatggaac agccttcttt caaggtgtgg cttttaaacc
     7381 aaactttgtc gaaaaatttg gtgaagaagt gggacttgag aagtttgatg ttatcaaacg
     7441 cttgagccaa gaaacgtctg tttatgtgac aggtattgtc aaagaggacg aacgttctaa
     7501 atttggctat gagttggaca tcacagacat cgaagtgatc ggtgaatctc aagactaccc
     7561 aatcacacca aaagaacacg gaacagactt tttgatggat aaccgtcact tgtggctacg
     7621 ctctcgtaag caagtagctg tgttgcaaat ccgtaacgct attatctatg caacttatga
     7681 gttctttgac aagaacggtt ttatgaagtt tgacagccca attctttcag gaaatgcggc
     7741 agaagattct acagaactct ttgaaactga ctacttcgga acgccagcct acttgagcca
     7801 atcaggtcag ctttacctag aagcaggggc tatggctctt ggtcgtgtct ttgactttgg
     7861 tccagttttc cgtgctgaaa aatcaaaaac acgccgtcac ttgactgagt tctggatgat
     7921 ggatgctgag tactcatact tgacacatga tgagtcgctt gacttgcaag aagcttatgt
     7981 gaaagctctt ctacaaggtg ttcttgaccg cgcgcctcaa gccttggaaa ccttggaacg
     8041 tgatacagaa ctcttgaaac gctacattgc agagccattc aaacgtatca cttacgatca
     8101 agccattgac ctcttgcaag agcatgaaaa tgatgaagat gctgactacg agcatcttga
     8161 gcatggtgat gactttgggt caccacacga aacttggatt tcaaaccact ttggtgtgcc
     8221 aacatttgtc atgaactatc cagcagccat caaggccttc tacatgaaac cagttcctgg
     8281 aaatccagag cgcgtgcttt gtgcagactt gcttgctcca gaaggctatg gagaaattat
     8341 cggtgggtct atgcgtgagg aagattacga tgcccttgtc gctaagatgg atgaacttgg
     8401 catggatcgt acagaatatg aattctacct tgaccttcgt aaatacggta cagttccaca
     8461 cggaggattt ggtatcggta tcgaacgtat ggtaaccttc gcagcaggaa caaaacatat
     8521 ccgtgaagct attccattcc cacgtatgtt gcaccgtatc aaaccataag aacaggaaaa
     8581 cgcccatgtg gcgttttttc gaattctagt gtttttactt gcgtaaaaaa attttttcta
     8641 gtataatagt ttattgtgag cgaacctcac ttaccccttg caaagtcttg gggtcattag
     8701 accaaaagga ggaacatatc aatggctaaa tacgaaattc tttatatcat tcgtccaaac
     8761 attgaagaag aagctaaaaa cgctttggta gcacgttttg actctatttt gactgacaac
     8821 ggtgcaactg ttgttgaatc aaaaacttgg gaaaaacgtc gtcttgcata cgaaatccaa
     8881 gatttccgtg aaggacttta ccacatcgtt aacgttgaag caaatgacga tgcagctctt
     8941 aaagagtttg accgtctttc aaaaatcaac gctgacattc ttcgtcacat gatcgtcaaa
     9001 attgacgcgt aagaaggtaa actatgatta acaatgttgt acttgtaggg cgtatgacac
     9061 gtgacgctga gttgcgttat accccatcaa atgtagcagt tgcgactttt actcttgcag
     9121 taaaccgtac atttaagagt caaaatggtg aacgtgaggc tgattttatc aatgtcgtta
     9181 tgtggcgcca acaggctgaa aatcttgcta actgggctaa aaaaggctca cttatcgggg
     9241 tgacaggtcg tatccagact cgtagttacg ataaccagca aggacaacgt gtctacgtga
     9301 cagaggtcgt ggctgagaat ttccaaatgt tggaaagccg tagtgtgcgt gagggccaca
     9361 caggtggagc ttactctgca ccaactgcaa actattcagc acctacaaat tcagtaccag
     9421 acttttcacg taatgaaaat ccatttggag caacaaaccc attggatatt tcagatgatg
     9481 atttaccatt ctaatggaca actaaactta taaaggagaa aaaacatggc tcaacaacgt
     9541 cgtggcggat tcaaacgccg taaaaaagtt gattacatcg cagcaaacaa aattgaatat
     9601 gttgattaca aagatactga gcttcttagc cgtttcgttt cagaacgtgg gaaaatcctt
     9661 cctcgtcgtg taacaggaac ttcagctaaa aaccaacgta aagtaacaac agctatcaaa
     9721 cgcgctcgcg taatggcttt gatgcctttc gtaaacgaag attaaagaaa agacccggtt
     9781 gggtctcttt ttcatgagct tgaaaacaag aaagcgaatt aagaccccaa cgggtctttt
     9841 ttcacaagct ttgaaatgag agagcgagtt aggctcagtg gctccctttt tacgagtttg
     9901 aaaacaagag aacacattaa aagcacgatg gagctttttc ataaatatgt cttttttctc
     9961 atccaagaaa ttaaaataaa aatgataaac tttttgaaaa taggggattt gcctacttta
    10021 tgatataatg gaaagagtta gattgaaaga ggtagtgaga tggacgcgaa attaagatac
    10081 aaggcaaaga agatcaagat tgtctttttt gatattgatg atacattgcg gaattcaaag
    10141 acagggttta ttccaactac aattcccact gtttttaaac agttgcgtga aaaaggaatt
    10201 ttaacaggaa tcgcctctgg acgtggcatt tttggtgttg ttccagagat tcgtgatctc
    10261 aagcctgact tttttgtaac tttgaatgga gcttatatcg aagataaaaa aggtcaggtc
    10321 atttatcagc atcagattga aaagtcagat gttgaggagt atatctcttg ggctaagcaa
    10381 gaaggaattg agtatggctt ggttgggagt catgatgcca agttgtcgac tcgcaccgat
    10441 atgatgagtg aagctatcaa tccaatttat cccgacttag atgtagatcc cgatttccat
    10501 gaaaaagaag atatctatca gatgtggact tttgaagata agggagatga cttgcacttg
    10561 cctgacagtc tctcagacaa acttcgcatg gttcgttggc atcaacattc gtctgatatt
    10621 gtgccgattt caggctccaa agcgacgggg gtggaaaagg ttgtggaaca ccttggcttg
    10681 aaaccagaga aggtcatggt ttttggagat ggtctcaacg acttggaact ctttgattat
    10741 gctggaatca gcgttgccat gggaatttct catgataaaa tcaaagaaaa agcagattat
    10801 attacaaaaa cattagaaga agatggcgtt tttgatgcct tagaagtatt tggtatggta
    10861 gaaaaagaat tgcattttcc acaagtagac attgaaacag tagaaggtcc tcttgcgact
    10921 attaagacca atcacggaga cttacgtatc aagcttttcc ctgaacatgc tcctaaaaca
    10981 gtggctaact ttgtatctct ttcaaaagat ggctactatg atggtgtcat tttccaccgt
    11041 attatcaagg actttatgat ccaaggtgga gacccaactg gaactggtat gggtggcgag
    11101 tcaatctacg gcgaatcttt tgaggatgaa ttctcagaag agctttacaa tatccgtggt
    11161 gctctttcca tggcaaatgc tggtccaaat accaacggca gccagttctt tatcgtgcaa
    11221 aaccaacacc taccttattc gaagaaagaa attactcgtg gtggttggcc agaaccgatt
    11281 gcagaaatct atgccaatca aggtgggaca cctcacctag accgccgtca cacggttttt
    11341 ggtcagttag ctgatgaagc atcttacgct gtcttggatg ccattgctgc tgttgagaca
    11401 ggagctatgg acaagccagt tgaagatgtt gtaattgaaa ctattgaaat cgaggactaa
    11461 gatgaaaatc ggtgataagc taaaggggcg tattacaggg attcagccct acggtgcctt
    11521 tgttgagtta gagacgggtg atacggggct gattcacatt tcagaaattc ggacaggatt
    11581 tattgaaaat attcatgaaa ccttgaaagt cgatgaagag gttcaagttc aggtagtgga
    11641 tttagatgaa tttacgggga aagccagtct ttctatccgc actttggagg aagaaaagta
    11701 ccagtttcct agacggagac gcttttcaag cgaccgcttt aactacggtt ttgctccctt
    11761 tcgacgaatg ctaccaatct ggactggaga agccctgcac catttaaaaa agaagaagta
    11821 gtttagagtt ttatattttt tgtaatgtta atatgaattt gctataatag gaccatggaa
    11881 gaagaacaat tattaaaatc aggagagcgc attaaccagc tcttttcgac agatatcaaa
    11941 atcattcaaa atagagaggt ttttagctat tcggtggata gtgttctctt atcacgattt
    12001 ccacgttttc ctaagaaggg gttgattgtg gatttctgtg ctgggaatgg agcagtgggg
    12061 ctttttgcta gcactcgtac tcaagcacag atattgtctg ttgagattca ggagcgtttg
    12121 gcggatatgg ctgaacgctc tgtccgtttg aatggattag aggagcaaat gcaggtcatc
    12181 tgcgatgatt tgaaaaatat gcctgctcac attcagggga gtaaggtgga tatgattttg
    12241 tgtaatccac cctatttcaa ggtgaatcct tattctaatc tgaacgagag tgaacattat
    12301 ctcttggctc gacacgaaat cacgactaat ttggaagaaa tctgtcgtag tgcccagagt
    12361 attctcaagt ctaatgggcg tttggccatg gttcatcgtc ctgatcgact tctggatatt
    12421 ttggatacgt taaaacgaca taatctagcc cctaagcgcc tgcagtttgt ttatccaaaa
    12481 agagaaaagg aagccaatat gcttttgatt gaggcgatca aggatggctc aacaagtggc
    12541 tttaaggtct tacctccttt gattgtccac aatgatgatg gctcttatac gcccgaaatc
    12601 gaagagattt attatggatc ataaggctta tatgtacgtt ctggagtgtc gtgatggatc
    12661 ctactatata ggctatacga ctgatatgag aagacgcctc gctatccaca atagtgggaa
    12721 gggagccaaa tatacacgag cacgcttgcc agtcaaactt atctatgctc aaggttttgc
    12781 cagtaaggaa gaagccatgt cggctgaagc tcttctcaag cgtaagaaga ggccacagaa
    12841 ggaagaattt ttatctgaaa atcaagatag aaatttactc agttattttg aagaatcgtg
    12901 gggagtcctt tgactcctct tacctttgtc aaaaagcgaa aaaaatgcta caataaagaa
    12961 aataaacaaa atgaggtatt atcatgtcca agattctagt atttggtcac caaaatccag
    13021 actcagatgc catcggatca tctgtagctt ttgcctacct tgcaaaagaa gcttacggtt
    13081 tggatacgga agctgttgcc cttggaactc caaatgaaga aacagccttt gtcttgaact
    13141 attttggtgt ggaagcacca cgtgttatca cttctgccaa agcagagggg gcagagcaag
    13201 ttatcttgac tgaccacaat gaattccaac aatctgtatc agatatcgct gaagtagaag
    13261 tttacggtgt tgtagaccac caccgtgtgg ctaactttga aactgcaagc ccactttaca
    13321 tgcgtttgga gccagttgga tcagcgtctt caatcgttta ccgtatgttc aaagaacatg
    13381 gtgtagctgt gcctaaagag attgcaggtt tgatgctttc aggtttgatt tcagataccc
    13441 ttcttttgaa atcaccaaca acacacccaa cagataaaat cattgctcct gaattggctg
    13501 aattggctgg tgtgaacttg gaagaatatg gtttggcaat gttgaaagct ggtaccaact
    13561 tggctagcaa atctgctgaa gaattgattg atatcgatgc taagactttt gaactcaacg
    13621 gaaataatgt ccgtgttgcc caagtgaaca cagttgacat cgctgaagtt ttggaacgcc
    13681 aagcagaaat tgaagctgca atgcaagctg ccaacgaatc aaacggctac tctgactttg
    13741 tcttgatgat tacagatatc gtcaactcaa actcagaaat cttggctctt ggtgccaata
    13801 tggacaaggt cgaagcggct ttcaacttca aacttgaaaa caatcatgcc ttccttgctg
    13861 gtgccgtttc acgtaagaaa caagtggtac ctcaattgac tgaaagcttt aatgcgtaag
    13921 attttgggtg tcagctcaaa atcggaaagt ctagtttgcc ttatatcgaa aggagtttcg
    13981 gctccttttt tctaggagtg aagtatgtta gaaaatggcg atttgatttt tgtgagagat
    14041 gggtcagaca tgggacaggc catccagact tccacaggta actatagcca tgttgccatt
    14101 tatttggatg ggatgattta tcatgctagt ggacaggctg gtgttgtctg tcaagaaccg
    14161 gcagacttct ttgagtccaa tcatttatac gacctctatg tttacccaga aatggatatc
    14221 cagtcggtga aggaaagagc ttgcaaacat cttggagcac cctacaatgc ttctttctat
    14281 ccagatgcac ctggtttcta ctgctcccag tatatagcag aaatcctacc tatttttgaa
    14341 actattccta tgaaatttgg agatggggag caggagatta gtgatttttg gagggagtat
    14401 tacatagaac taggtctgcc tgttcctctg aaccaagctg gtaccaatcc tagtcagttg
    14461 gcagcatcgc ctctgttaca atgtaaagaa aggaatcttc atgattcaga tttttaatcc
    14521 atctcgtttg acgagacagc catttttgga gaattgatcc gctatctgga ccagtatgag
    14581 gatgtgattc tacgggaaat taaggctcaa tttccagatg ttgcagttga taaactcatg
    14641 gaagagtata taaaggcagg cttgattcta cgtgaaaata agcgctatta cctcaatttt
    14701 cctacgcttg aatcacttga tagtcttgaa ctggatcaag agatttttgt cagagaagct
    14761 agtccggtct atcaagcctt gttggagcag agttttgaga cggaattgcg caatcaaatc
    14821 aatgcagcta ttttagttga aaagacggac tttgcgcgca ttaaaatgac tctgtccaat
    14881 tatttttaca aggtcaaaca gcagtatcct ttgacagaaa aacagcagga gctctatgac
    14941 attttaggag atgttaatcc tgagtatgca ctcaagtata tgacggcttt tttgttgaaa
    15001 tttctcaaaa aagaccagct tatgcagaaa tgccgtgata tctttgtgga cagtttggtt
    15061 gtcttaggct atattgtgca aaatgaagat ggaaagtatg agttggctat cgattttgat
    15121 aaggagagat taactttcta cttagcgtga tttcttgttt ctgagtacat tgtttgactt
    15181 tccttagtat tcggtataaa ctatatgtaa ccggtaacac atatcggaat aaactaaagg
    15241 agacaatcat atgtcacttg aaaacaaatt ggaacaagca acaggcgctg tcaaagaagg
    15301 ttttggtaaa gttactggag acagcaagac agaacttgaa ggagctgttg aaaaaacagt
    15361 tgctaaggca aaagacgttg tagaagacgc aaaaggtgct gtagaaggtg ccgttgaagg
    15421 tttgaaaaac gtttttacta aagaatagga aaaaatcaag ggtttcattt tcccttgatt
    15481 ttttctattc ttataaataa ttttctgcga cggctgtatc tcctgggtag gattctttct
    15541 tgccctggat gatttggtaa caatcggctc ccttacccgc aataataact gcatctaatt
    15601 cgtgatttgt gatagccatt gccgccttga tggcttcttg gcgatccgca atcttctcaa
    15661 caggatgatt gatgtagcta ctaatttcat ctgcaatggc cattgggtct tcatagttag
    15721 ggtcatcagc agtcagaaag acttgaatct cagggtgttg attgaggagg aggccaaagt
    15781 ccttacgacg actttctccc ttgtttcctg ttgatcccag aaccagagca atctttccgg
    15841 tttgatgagt ttcaaccaca ttgatgagtt ttttcagact atccccattg tgggcatagt
    15901 cgatgaagac cttggctcca tttttctgag tgaggacttc catacgacca ggaacgcggg
    15961 ttgcagcgat gccttttttg atgtcctcaa gacttgctcc gagacggaga caagcaagtc
    16021 cagcagcaac tgcattttct tggttgaagt tgccaatgag ttggatatca taatctccag
    16081 cgagtttacc cgtagctgaa aagctaaagg ctttggaatt ctcgatttgg ttatcaaatt
    16141 ggctaccata gaaatcatgg tcttgatctt taacctgttc tttcaagact gagaagtggt
    16201 ccatgtcact gttaatgatg actgctcggc tattttccat caagagacgc ttgtggtaga
    16261 aatagtcttc aaagctaggg tgttcaatcg ggccgatatg gtctgggctg atattgagga
    16321 aaactcccac atcaaaggtt agaccataga cacgtctgac cagataggat tgactggaga
    16381 cttccatgat gaggtgggta cggtcatttt gcacagcctg attcatcatg tcaaagaggt
    16441 caatactctc aggggttgtc aacgctgact taaagaaagt ctcgccatca agagttgtgt
    16501 tcatggtcga caacatagca ggtcgatgcc cttgagataa gatgttatag gcgaaatagg
    16561 ctgctgttgt cttaccctta gtaccagtaa aggcaaggag tttgagtttt tcctgtggat
    16621 taccatagaa ctccatggca atcaaactca tggctttctt tatatcgttc acaatgatga
    16681 cagggatacc gacttcgtag tccttttcag ctacatacca agctaagcct tgtgttatag
    16741 cagaaagaag gtattctttt ttaaaggcag cgccttttgc aaaaaaaaga gtgtcttctg
    16801 ttacttttcg gctgtcgtag ctgatgctat caaaaacaac tttgctgtag ttgtagtggt
    16861 aatgaccttg gtcaataatt tcgcgaaaaa ggccatcttt ctttaaaata tctaatacag
    16921 tttcaatttt tatcatactt tctattgtaa accgaaagtc gcaaatttac aagtaacaag
    16981 gaaaagttta taatggaaga taaggagttt ttcctagtta tcaaaattga atgaggaatc
    17041 tatgtcgcac gaaaacaatc accagcaggc ccagatgtta cgggggactg cttggctaac
    17101 ggctagtaac tttatcagtc gcctactcgg ggctgtttac attatccctt ggtacatctg
    17161 gatgggggct tatgcagcta aggcaaatgg tctctttacc atgggttaca atatctatgc
    17221 ttggttcttg ttggtttcaa cagcggggat tccagttgcg gtggccaagc aagttgccaa
    17281 gtataatacc atgcgagaag aagagcatag ctttgccctg attcggagct tcttaggctt
    17341 tatgacagga ctaggcctgg tttttgcttt agtcttgtat gtctttgctc cttggctagc
    17401 agacttgtct ggcgtgggca aagacttgat cccaatcatg caaagcttgg cttggggagt
    17461 cttgattttc ccgtctatga gtgttatccg aggatttttc caagggatga ataacctcaa
    17521 accctatgcc atgagccaaa ttgctgagca ggtcattcgt gttatctgga tgctcctagc
    17581 aacctttatc attatgaagc tcggttcagg agattatcta gcagccgtta cccaatcaac
    17641 ctttgctgcc tttgtcggta tggtagccag ttttgcagtc ttgatttatt tccttgccca
    17701 agaaggttca ctcaaaagag tctttgaaac aggagataag attaacagta agcgtctctt
    17761 ggttgatacc attaaggaag ccattccttt tatcctgaca gggtctgcca tccagctctt
    17821 ccagattttg gatcagctga cctttatcaa tagtatgagc tggtttacca actacagcaa
    17881 tgaggacttg gttgtcatgt tttcttattt ctcagccaat cctaataaaa tcacgatgat
    17941 tttgatttct gtaggggttt cgattgggag tgttggtttg ccacttttga cggaaaacta
    18001 tgtcaagggg gacttgaaag cagcttctcg tctggttcag gacagtctca ccctactctt
    18061 tatgttcttg ctaccagcaa cggttggagt ggttatggta ggagaacctc tatatacagt
    18121 cttctatggt aagccagata gtttggctct gggcttattt gtctttgcag ttttgcagtc
    18181 tattatttta ggcttgtaca tggtcttgtc tccaatgctt caggccatgt tccgcaaccg
    18241 caaggccgtt ctctatttta tctatggttc tattgccaag ctagtcttgc aactacctac
    18301 catcgccctc ttccacagtt atggtccttt gatttcaaca accattgctc tcatcattcc
    18361 taacgtcttg atgtatcggg atatttgtaa agtaactggt gtcaagcgca aggtgatttt
    18421 gaagcgaacc attttaatca gtttgctgac cctagtcatg tttctgttaa taggaaccat
    18481 ccagtggctg ttaggatttt tcttccaacc aagtggacgt ttgtgtagct tcttttatgt
    18541 agctcttgtc ggtgccatgg ggggtggact ttatatggtt atgagtctgc gtacctattt
    18601 attagataag gtaataggaa aagcccaagc agatcgcctg cgagcaaaat ttaagctttc
    18661 gtaaggtagc atttataaat aaaaggaata agttgaacaa tcttttagga gaacttgaag
    18721 cttattcctt ttttagcgta aaaatagcta aaattatatt tcaaaaatag aaaatgaggc
    18781 ttttttcgat tttttggaaa aaatcattga ctaaacaaac taaacgttgt ataataaaac
    18841 aaatatttaa agcaggaggt tccggaaatg aaaaagtcta agagcaaata tctaaccttg
    18901 gcaggtcttg tcctgggtac aggagtttta ttgagcgcgt gtggaaattc tagcacggcg
    18961 tcaaaaacat acaactatgt ttattcaagt gatccatcta gcttgaacta tctagcagaa
    19021 aaccgcgcag caacatccga tattgttgca aatttggtag acgggttatt agaaaatgac
    19081 caatatggga atattattcc atcattagca gaggattgga ctgtttctca ggacggtttg
    19141 acctatacct acaaacttcg taaggatgcc aagtggttta cttctgaggg agaagaatat
    19201 gcgcctgtaa ctgcccagga ttttgtgaca ggtttgcaat atgcagctga taaaaaatca
    19261 gaagccttgt atctagtgca ggactctgtt gctggtttgg atgactatat cactggtaaa
    19321 acaagcgact tttcaactgt cggtgtcaag gcacttgatg accaaacggt tcaatatact
    19381 ttggttaaac cagaacttta ctggaattca aaaacacttg caacgatact ttttcctgtt
    19441 aatgcagatt tcctgaaatc aaaaggggat gattttggga aggcggatcc atctagtatt
    19501 ttgtacaatg gacctttctt gatgaaagca cttgtctcaa aatctgctat tgaatataag
    19561 aaaaacccta attactggga tgctaagaat gtctttgtag acgatgtgaa attgacctac
    19621 tatgatggta gcgaccaaga atcactggaa cgtaatttta cagctggtgc ttatactacg
    19681 gctcgtcttt ttcctaacag ctccagctat gaagggatta aagaaaaata caaaaacaat
    19741 atcatctata gtgtgcaaaa ttcaacttca tatttcttta attttaacct agataggaag
    19801 tcttacaatt atacttctaa aacaagtgac attgaaaaga aatcgaccca ggaagcagtt
    19861 ctcaataaaa acttccgtca ggctatcaat tttgcttttg acagaacatc ttatggggct
    19921 cagtctgaag ggaaagaagg tgcaacaaag attttgcgta acctagtggt tcctccaaac
    19981 tttgtcagta tcaagggaaa agactttggt gaagttgtag cctctaagat ggtcaactat
    20041 ggtaaggaat ggcaaggtat caactttgcg gatggtcaag acccttacta caatcctgag
    20101 aaagccaagg ctaagtttgc ggaagctaag aaagaactcg aagcaaaggg tgttcaattc
    20161 ccaatccact tggataagac tgtggaagta acagataaag taggcataca aggagttagt
    20221 tctatcaaac aatcaattga atctgtttta ggttctgata atgtagtgat tgacattcag
    20281 caattaacat cagatgagtt tgacagttca ggctactttg ctcaaacagc tgctcagaaa
    20341 gactatgatt tatatcatgg cggttgggga cctgattatc aagacccgtc aacctatctc
    20401 gatattttta atactaatag tggaggagtt ctacaaaatc ttggactaga gcctggagaa
    20461 gccaatgaca aggctaaggc agttggactg gatgtctata ctcaaatgtt ggaagaagct
    20521 aataaagagc aagatccagc caaacgttat gagaaatatg ctgatattca agcttggttg
    20581 attgatagtt ctttagttct tccaagtgtt tcgcgtgggg gaacaccatc attgagaaga
    20641 accgtaccat ttgctgctgc ctatggttta accggtacaa aaggggttga atcatataaa
    20701 tacctcaaag tacaagataa gattgtcaca acagacgaat atgcaaaagc cagagaaaaa
    20761 tggttgaaag aaaaagaaga atccaataaa aaagcccaag aagaattggc aaaacatgtc
    20821 aaataaaatc taattgtaga taagtttgtg taagaagtct ttgtgcttaa aaacgcataa
    20881 tatcaggtat ataaaatcct agatgttatg cgttttgtta taggaagatt tattagattt
    20941 tatcctcaaa ttgagttatt cccgacttta tccgttgaaa tattataagg agtatttatg
    21001 aaattaaaat tattaagagt agatactaag gtgattatgg ggagtttctt acttgttctg
    21061 tctagtctac ttgctttgtt gcttcccctt atcttaaagg gtttaataga tgggagttct
    21121 attgaaaata taggttccaa agtatttcaa tcgtttttga tttttattgg tcaagccttg
    21181 ttttcttcta ttggttacta tctgtttagt caatcgggtg aaaaaagata gcaaaaatca
    21241 ggaaaaaagt gatagagggg ttgatttatg cagagaaatc cttctttgat aagagccaaa
    21301 gtggggagtt gacttctgcc attgtcaatg acatgagtgt cattcgtgag tttttaatta
    21361 cgactttccc aaatattatt ctgagtttag ttatggtact tggttccatt gtagtcttat
    21421 ttagtcttga ttggaatctt tctctacttt tattcatcac tcttccttgt atgatgttta
    21481 ttatcttgcc cctttccaat atcagtgaaa agtatagtcg tcgtttacag gaggaaatcg
    21541 gatttttaac agggcaattg actgaaaaga ttcaagaaca tgaattaatt aagactaacc
    21601 aagcagagaa aagtgtacag gatgttttgg ataattgtat tgaaagggta caaaacaatt
    21661 cactgaaatc agatagggtt acttcttttg agaccccgtt tgctctctta tttatctttg
    21721 cgactatagt tgtgatgcta acctatgggg gttatcgggt cagcgcagga tatatatctg
    21781 tgggaacctt ggtttcgttt ttgatttacc tctttcaatt acttaatcct attagtaata
    21841 tagctaattt tgtaactatt tattctagta gcaagggatc ttcagttgca ctggagaact
    21901 tgcttgcagt tcctaaagaa aaatttgagg gaggaaaatc ggtatcagga caagggttga
    21961 attttaacca tgtctatttt ggttatgatg aaaatcgacc tgtcttaaag gatattactt
    22021 gttcaatttt caaggggcaa aaaattgctt ttgttgggcc atctggatca ggaaaatcaa
    22081 cgattgtgcg tttgttagag cggttttata aaccgctttc aggagatatt ctaatggagc
    22141 aatcaagtat atatgatttt aacttaaaag aatggagaag taaaatcgct tgggtttcac
    22201 aaaataatgc agtcttatct ggcagtattc gtgacaatct ttgtctcggt ttgaatcgct
    22261 tagtaactga tgatgaattg atgaaagtgc tagacttagt atcactaggt gatgagattc
    22321 gctccatgaa agagggacta gatactgaag ttggtgaacg cggacgactc ttgtcagggg
    22381 ggcgaagcca aagacttcaa atagctagag cctacttaaa agatgctgaa attcttatat
    22441 ttgatgaagc cactgctaat cttgatgcgg attctgagta tgcgattatc agtagcctct
    22501 attctgtact aaaggagaag acggttgtga ttatagcgca tcgcttgtca acggtaaaag
    22561 atgtggattg tattttcttc ttagaggagg ggaaaatcac tggctcagga actcataagg
    22621 aactactgga aaatcatgag cgttatgctc gttttgtgca ggagcaaatg atagagtgaa
    22681 ggtgtctttt gagattcacc attttatagt ctattaaagg gagcaggaaa aactcccttt
    22741 ttatatagtt tgaaactata actagctctt gaaaagaaga aaatgagttg atgaaaataa
    22801 gtggtacaat agttactata gatttggagg aattgtatga gcaaggaatt acacattaac
    22861 acaattttag cacaagcagg gatcaagtct gatgaggcaa caggtgcttt ggtgacacca
    22921 cttcattttt caacgaccta tcagcatcca gagtttggtc gatctactgg gtttgactat
    22981 acgcgcacta aaaatccaac tcgtagtaag gctgaggaag tcttggcggc tattgagtca
    23041 gcagactatg ccctagcgac tagctcaggg atgtcagcta ttgtactggc ctttagcgtc
    23101 tttccagtag gaagtaaggt cttggcagtg cgtgatcttt acggtggttc ttttcgctgg
    23161 tttaaccaag tggagcagga aggtcatttc cattttacct atgccaatac agaagaagag
    23221 ttgattgccg agttagaaaa ggatgtggat gttctctata tcgaaacccc aaccaatccc
    23281 ttgatgttgg aatttgatat cgaaaaacta gcaaaattgg ctcatgctaa gggtgccaaa
    23341 gtggtggtgg acaatacctt ctatagccct atctaccaac gtccgattga agatggagca
    23401 gatatcgttc tccattcagc aaccaagtat ctagcaggcc acaatgatgt cttggctgga
    23461 gtggttgtga ccaatagttt agaactatac gagaagcttt tttacaatct taatacaaca
    23521 ggggcagtct tgtctccatt tgacagctac cagttgcttc gtggtctcaa gaccttgtct
    23581 cttcgtatgg agcgctcaac agctaacgcc caagaagtgg ttgccttttt gaaggattct
    23641 ccagcagtta aggaagttct ctacactggt cgtggaggca tgatttcctt taaagtagcc
    23701 gatgaaacac gcattcctca tattttgaac agtctcaagg tcttctcttt tgcggaaagt
    23761 ttgggtggag tggaaagtct tattacttat ccaacgactc aaactcatgc tgatattcca
    23821 gcagaagtac gccattctta tggtttgaca gatgacctct tgcgtttgtc tattgggatt
    23881 gaggatgcta gagatttgat tgcagatttg cgccaagcct tagaaggata agacaaagat
    23941 gggaaaatat gattttacaa gcctgcccaa ccgtttaggg caccatacct ataaatggaa
    24001 agaaacagaa acggatagtg aagttctacc agcttggata gcggatatgg actttgtagt
    24061 cttgcctgaa atccaccaag ccgtgcaaac ttacgcagac caactggttt atggttatac
    24121 ctatgccagt gaagacttaa ttaaggaagt tcaaaagtgg gaagctacac aatacggtta
    24181 caactttgac aaagaggctc ttgtctttat cgagggtgtg gtaccagcca tctcaacagc
    24241 tattcaaacc tttacaaaag aaggcgaggc ggttttaatt aacacgcctg tctacccacc
    24301 ctttgctcgc agtgtcaagt tgaataatcg tagattgatt actaattcct tagtggaaaa
    24361 ggatagtctg tttgagattg actttgacca acttgaaaag gatttggtgg aagaggaggt
    24421 taaactctat gttctttgca accctcacaa tcctggtgga cgtgtttggg aaaaagaagt
    24481 gttggagaag attggccaac tctgccaaaa acacggtgtt ttgttagttt cggatgagat
    24541 tcaccaagat ttgaccctct ttggtcacaa acaccagtct ttcaatacca tcaatcctgc
    24601 cttcaaaaat tttgctatcg tcttgagcag tgccactaaa acatttaata ttgctggaac
    24661 aaaaaattcc tatgcagtca ttgaaaatcc taagttgaga ctggctttcc agaaacgcct
    24721 gttggccaat aatcagcatg aaatttcagg cttgggttat ttggcgacag aagctgccta
    24781 tcgatacggt aaagattggc tagaggaact caagcaagtc tttgaagacc acatcaatta
    24841 tgtggtggat ctatttggaa aagagactaa aatcaaggtc atgaaaccgc aaggtaccta
    24901 cttgatttgg cttgactttt cagcttatga cctgactgat gaaacattgc aagagttgtt
    24961 gagaaatgaa gccaaggtta tcctcaaccg tggtttggat tttggagagg aaggaagtct
    25021 ccatgcccgc atcaatatag ctatgcccaa atctctgttg caagaagtct gtcagcggat
    25081 tgtggctact tttgccaaat gttaaaaatc cagccttcta ggagaaaagt cttcctagaa
    25141 ggctattttc ataggcgaaa atatggtata ataaaaagat aaggtaaagg tgaaaatatg
    25201 gctaaattga ttccggggaa aattcgtatc gaaggtgttg ccctttatga aactggtaag
    25261 gttgatatca tcaaggaaaa gaacaatcgg ctctacgctc gcgttgcaga agaagaactg
    25321 cgctatagtt tagaggatga tttgattttt tgtgcctgtg atttttttca aaagaggggc
    25381 tactgtgtgc atttggcagc gctagagcat tttctgaaaa atgatgagcg tggtcaggaa
    25441 atcttgtgga gtctggaaga aggtcatgaa gaaaaagagg ccgttgaaac caaggtgacc
    25501 ttgggtggca agtttttgga tcgaatttta tctccaaaat cagaatgcgc ctatgagtta
    25561 tcagctgtgg gtcaggtgga agcaggaacc aatcatatct tgtggactct gagaatcggc
    25621 caaatcaata gccaaaaata ctatgtcatt cgggatattc cacttttttt aaggattgtc
    25681 gagcagagaa agtcgtatat gattggcaag atttatgagg agtctctttc ttgggaagcc
    25741 tttgatgatg ccagccaaga acttttgact ttcttgcacg gactaacgga ggaagggctg
    25801 gttccagacc tctttttcca aaaccaaggt cggcacctct ttttccctct gacctttttt
    25861 gagcagggtg tgaatttact gatggccttg ccccattttc aatttgacca tcaagtggat
    25921 agctaccaaa ccctgctttt tcaggatatg catgctgatg ccaatctatt tgcctttaca
    25981 gtaacagaat attcggacta ttttgaaatg gaaatcagtg agagtcctag ggtcaatgtc
    26041 ttttatcaag gagcagtact tttccataaa ggtcaggttt attttttaac agaccagcag
    26101 atgcgtcttc tcaaggaaat taaagcgtta cctgtggatc gacaaggaaa gaaatacctg
    26161 caatttgata gcagtgatcg agacaaattg gcctcctgtc tgactctttt tatccagatg
    26221 ggcaccgttt cagctccaga acgtctacaa atcaaaactt ttgcgccctc tttttacttt
    26281 gacagggaag aagataatcg gattcgttta gaaattcagt ttgattatgg aaatagacag
    26341 gtatctagcc gacaagagct agaagaatta ccattttcaa gtgacgcgga cttagaagaa
    26401 agaattttcc aagtctgttt ggctgcgggc tttgaagccg attttcaatc ttggcgtcag
    26461 gccttaaaag cggagtctgt ctatcatttt tttcatgata tcattccagt ctttgaaaaa
    26521 ctcgggcatg ttgacctatc agataaacta gaagagcttt atagcctagc gagtcctcag
    26581 gtgcagattg cctccaaggg aggtctcttg gaaatccagt ttgattttca agatattgcc
    26641 caggaggaaa tcgaccaagc catacaggcc ttggttgcca atcaggattt ttatattgat
    26701 tcgtctaatc aagtttactt tttcgatgaa gaaaccaaga aaattcgcca aaatctacag
    26761 gaactggggc aatttgaatt aaaagatggg accttgcagg ctcggaaatc cttggcctac
    26821 agtttagctc atctctttga agggcgagac cgtgtttctt tttcacaaga attccagaat
    26881 ctggcccagg atttgacgca tccagaggac tttcctttgc aagcaactca ggtcaaggct
    26941 gacttgcgag attatcagga aaagggaatt ggctggttgc agatgctcca tcattatggt
    27001 tttggtggga ttttggctga tgatatggga cttgggaaaa cccttcagac cattgctttt
    27061 ttgactagtc aagtgacaaa agaaagtcgg gttttgattc tcgctccgtc gggtttgatt
    27121 tacaactggg cagatgagtt tcagaaattt gctccacagt tggatgtggc tgttgttcat
    27181 ggtttgaaag caagtcgtga ggagattctt gccgagagcc atcaaatcta tgtgactagc
    27241 tatgccacct tccgtcagga cagtgagctt tatcagggga tggcctttga cttccttttc
    27301 ttagatgaag ctcaggtcat gaaaaatgcc cagaccaaga ttgcccagac cttgagacaa
    27361 tttgtggtgc cgtcggtctt tgccttgtca ggaactccga ttgaaaacca tctgggtgag
    27421 ttgtggtcta tttttcaaat cgtcatgccc ggacttttgc caagcaagaa agaatttatg
    27481 aaattgccag cagagcgagt ggctcagttt atcaagcctt tcgtgatgcg acgcaagaaa
    27541 gaagaagttc tgactgaatt gccagacttg attgaggtgg tttataaaaa tgaactggaa
    27601 gaccagcaaa aggctattta ccttgcccag ttacaacaga tgcgagacca tctggctcaa
    27661 gtgtcagaac aggaatttca gcgaagtcgt gtggaaattt tatctggttt gatgcgcttg
    27721 cgtcaaatat gtgacactcc tgccctgttt atggaagatt atcagggagc cagcggcaaa
    27781 ctagatagtc tccgagacct actggtacag gtggcagacg gcggacaccg tgtcttgatt
    27841 ttctctcagt tcaagggaat gttggaaaaa attgaacaag aactgccaga cttgggcctg
    27901 acatccttta aaattacggg ttcaacccca gccaaggaaa gacaagacat gaccaaggcc
    27961 tttaaccaag gagaaagaga tgcctttctg atttccctca aggctggtgg ggtcggtctg
    28021 aacctgacag gtgcagatac agtgatcttg gttgaccttt ggtggaatcc tgcggtggaa
    28081 gcgcaagcca ttggccgtgc ccatcggatg ggtcaggaag aaacggttga ggtctatcgc
    28141 ttggtgacca agggaaccat tgaagaaaaa attcaggaac tccaagaaca aaagaaacat
    28201 ctggtgtcac aagtattgga cggcacagag tcacgtggta gtctgaccct agcagaaatt
    28261 agagaaattt tgggaatttc tgaagccagc acttgaaaaa tcaagacaat acgctataat
    28321 gaagtgttga aaggaaatag aaaatgaaac aagaacgatt tccattggtg tcagatgacg
    28381 aggtcatgtt gactgaaatg ccagtcatga atctctatga tgagtctgat ctgatcagta
    28441 atatcaaggg tgagtatcga gataaaaatt atttagaatg ggctcctatt gctgaagaaa
    28501 aaccagtaaa accgattgaa aagcaagtag ggaaacctaa aaaagctcct ttaggggtta
    28561 aaaaagaagg aaagagctat gcggaggtgg cgcgtgaaga agcgcgtgca gacttgaaaa
    28621 agaaacgctc tgctaactat ctaactcagg atttcagcct tgcgagacgt cattctcagc
    28681 ccagtctagt tagacagggc aatcaaccga caactccttt ccaaaaggaa aatcctggtg
    28741 aatttgtcaa atatagccaa aaattgaccc agtctcatta tatcttggcg gaagaagttc
    28801 attctatccc taccaagaat gaagaagtgt cagcacctgc tccaaagaaa aacaattatg
    28861 attttctaaa gaagagccaa atctacaata aaaaaagtaa acaaacagaa caagaacgtc
    28921 gggttgccca agagttgaat ctgaccagaa tgacagaata ggggagaaaa catgtcaaag
    28981 acatatcatt ttatcggaat taagggatca gggatgagtg ccttggcctt gatgttgtac
    29041 cagatggggc acaaggttca gggatcagat gttgaaaagt actactttac ccaacgcggt
    29101 cttgagcagg caggaattac cattcttcct tttgatgaaa agaatctaga cggtgatatg
    29161 gaaattatcg ctggaaatgc ctttcgtcca gataacaacg tcgaaattgc ctatgcggac
    29221 caaaatggta tcagctacaa acgttaccat gagtttctag gtagctttat gcgtgacttt
    29281 gttagcatgg gagtagcagg agcacatgga aaaacttcaa cgacaggtat gttgtctcat
    29341 gtcttgtctc acattacaga taccagcttc ttgattggag atgggacagg tcgtggttcg
    29401 gccaatgcca aatattttgt ctttgaatct gacgaatatg agcgtcactt catgccttac
    29461 cacccagaat actctattat caccaacatt gactttgacc atccagatta tttcacaagt
    29521 ctcgaggatg ttttcaatgc ctttaacgac tatgccaaac aaatcaccaa gggtcttttt
    29581 gtctatggtg aagatgctga attgcgtaag attacgtctg atgcaccaat ttattattat
    29641 ggttttgaag ctgaaggcaa tgactttgta gctagtgatc ttcttcgttc aacaactggt
    29701 tcaaccttca ccgttcattt ccgtggacaa aacttggggc aattccacat tccaaccttt
    29761 ggtcgtcaca atatcatgaa tgcgacagcc gttattggtc ttctttacac agcaggattt
    29821 gatttgaact tggtgcgtga gcacttgaaa acattttccg gtgttaaacg tcgtttcact
    29881 gagaaaattg tcaatgatac agtgattatc gatgactttg cccatcatcc aacagaaatt
    29941 attgcgacct tggatgcggc tcgtcagaaa tacccaagca aggaaattgt agcagtcttt
    30001 caaccgcata cctttacaag aaccattgcc ttgttggacg actttgccca tgctttaaac
    30061 caagcagatg ctgtttatct agcgcaaatt tatggctcgg ctcgtgaagt agatcatggt
    30121 gacgttaagg tagaagacct agccaacaaa atcaacaaaa aacaccaagt gattactgtt
    30181 gaaaatgttt ctccactcct agaccatgac aatgctgttt acgtctttat gggagcagga
    30241 gacatccaaa cctatgaata ctcatttgag cgtctcttgt ctaacttgac aagcaatgtt
    30301 caataggatg ttcccatgga aattccaatt aagatcattc aggcaagcaa gtctgatttg
    30361 cctgagatag gggcacttca gacctcgtct tttccagctg aaaagcagca actttcccat
    30421 attttagaag aaagtatccg taagtgtgcg gatacctttc ttctagctag ggatgaaaat
    30481 caacttttag gctatatttt atcaagtccc cagtcagaca atccgcaata tctaaaagta
    30541 cattctttag tcatcgagtc tgaccatcag agacagggct tgggaacact tcttcttgca
    30601 gccttgaaag aggtggcagt tgagctggat tacaaaggga ttcgtttgga gagtcctgat
    30661 gagctgcttt cctattttga aatgaacggt tttgttgatg aagaagcaac tttgctctat
    30721 gcaactagcc agggctatag tatgatttgg tttaatccct tttatctgga ggaacaatga
    30781 aaatcagaca agcaagatta gaagatttgg atcggattgt tgaacttgaa tttgaaaatt
    30841 tttcggtcga agaagccatt cctccttctg tctttgaagc acatttgaga gaaattcaga
    30901 cctcttttct ggttgctgaa aaagaaggaa gaatcatggg ttatatcgaa ggaccagttg
    30961 gcctgcaccg ccatctgcaa gaccagtctt ttacagaaga aataaaagac tatagtcatg
    31021 agcctggtgg ttatatctct gtgacctgtc tgtctattgc caaggaggca cagggactcg
    31081 gactgggtca gaaattgctg acagccttga aagaagttgc tcttgaagat gagagagatg
    31141 gcattaatct aacctgtcat gactatctca tcgcctacta tgaaaaacat ggatttgtca
    31201 acgaaggcca gtcccagtca acctttgcag gggaaacatg gtatgatatg gtctgggaaa
    31261 tgaaaaaata agttaggaaa agtatcatag attgcttttt ttggactttt aagatataat
    31321 attatggatt atcaacaagg aggaaaaact tttgagtgaa aagtcaagag aagaagagaa
    31381 attaagcttt aaagagcaga ttctgagaga tttagaaaaa gtaaaaggct atgatgaagt
    31441 tctgaaagaa gatgaggcag tagttcgcac tcctgcaaat gaaccttcaa ctgaagaact
    31501 catggctgat tccttgtcaa cggtagagga gattatgaga aaagctccta ccgtgcctac
    31561 tcacccaagt caaggtgtac cagcttctcc agcagatgag attcaaagag aaactcctgg
    31621 tgttccaagt catccaagtc aagatgtacc ttcttctcca gcggaagaaa gtggatcaag
    31681 accaggtcca ggtcctgtta gacctaagaa acttgaaaga gaatacaatg aaaccccaac
    31741 aagggtagct gtttcctata cgacggcaga gaaaaaagca gaacaagcag gtccagaaac
    31801 acctacgcct gctacagaaa cagtggatat catcagagat acatcacgtc gtagccgtag
    31861 agaaggagca aaacccgtta agcctaagaa agagaagaag tcacatgtga aagcttttgt
    31921 gatttcattc cttgtattcc ttgccttgct ctcagcaggt ggttactttg gttaccagta
    31981 cgtgctagat tccttattac ctatcgatgc taattctaag aaatatgtga cggttggaat
    32041 tccagaaggt tcaaacgttc aagaaatcgg tacgacgctt gaaaaagctg gtttggtaaa
    32101 gcatggtctg atttttagtt tttatgccaa gtataaaaat tataccgact tgaaagcagg
    32161 ttactacaat ttgcaaaaga gtatgagtac agaagactta ctcaaagagt tgcaaaaagg
    32221 tggaacagat gaaccgcaag aacctgtact tgcgactttg acaattccag aaggttatac
    32281 cttggatcag attgctcaag ctgtgggtca attgcaaggt gacttcaaag agtctttgac
    32341 agcggagact ttcttggcta aagttcaaga tgagacgttt atcagtcaag cagtagcgaa
    32401 atatcctact ttactggaaa gtttgcctgt aaaagacagc ggtgcgcgtt atcgtttgga
    32461 aggatacctt ttcccagcta catactctat caaggaaagc acaactattg agagcttgat
    32521 tgatgagatg ttagctgcta tggataagaa cctatctcct tactatagta ctatcaaatc
    32581 taaaaacttg actgtcaatg agttgttgac cattgcttcc ttggtcgaaa aagaaggtgc
    32641 caagacagaa gatcgtaagc tcattgcagg tgtattctac aatcgtttga atcgtgatat
    32701 gccacttcaa agtaatattg caatcttgta tgcccaagga aaactggggc aaaatatcag
    32761 tctagctgag gatgttgcga ttgataccaa cattgattca ccttataatg tttataaaaa
    32821 tgtaggtctc atgcctggtc cagtcgatag tccaagtctg gatgcgattg agtcaagcat
    32881 caatcaaact aagagcgata acctctactt tgtagcagat gtcacagaag gcaaggtcta
    32941 ctatgctaac aatcaagaag accacgaccg caatgtcgct gaacatgtca acagcaaatt
    33001 aaactaaaca aactaaaatt atgttatact tcacataatt ttctttagaa aatattatca
    33061 gaagaaagtt gagaaaaatg gcagaaaaaa catatcctat gacccttgag gaaaaggaaa
    33121 aacttgaaaa agaattagaa gaattgaaat tggttcgtcg accagaagtg gtagaacgca
    33181 ttaagattgc ccgttcatac ggtgaccttt cagaaaacag tgagtacgaa gcagctaagg
    33241 atgaacaagc ctttgtcgaa ggacaaatct ctagcttaga aacaaaaatc cgctatgctg
    33301 aaatcgtcaa tagcgacgca gttgcccagg acgaagtagc gattggtaaa acagtcacca
    33361 tccaagaaat tggtgaggac gaagaagaag tttatattat cgtaggttca gctggtgcgg
    33421 atgcctttgc aggtaaggtt tcaaatgaaa gcccaattgg gcaggccttg attggcaaga
    33481 aaacaggtga tacagcaacc attgaaacgc ctgttggtag ctatgatgta aaaatcttga
    33541 aggttgaaaa aacagcctaa aaacagaaaa aggagtgggg aggcgatgtg cttcactcac
    33601 tcctttttcc attttgctat tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttgc
    33661 cgtatgtatg gttactgact tcgtcagttt catctacaac ctcaaaacca tgttttgagc
    33721 cgacttcgtc agtttcatct acaacctcaa aaccatgctt tgagctgact tcgtcagttt
    33781 catctacaac ctcaaaacca tgctttgagc tgacttcgtc agtttcatct acaacctcaa
    33841 aaccatgctt tgagctgact tcgtcagttc tatctacaac ctca
//
LOCUS       DAVLVF010000018        32924 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVLVF010000018 DAVLVF010000000
VERSION     DAVLVF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32924
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.018"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(256..1818)
                     /gene="guaA"
                     /locus_tag="VYN66_000779"
     CDS             complement(256..1818)
                     /gene="guaA"
                     /locus_tag="VYN66_000779"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV1494840.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CLYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1960..2658
                     /locus_tag="VYN66_000780"
     CDS             1960..2658
                     /locus_tag="VYN66_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1494841.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2701..3597)
                     /locus_tag="VYN66_000781"
     CDS             complement(2701..3597)
                     /locus_tag="VYN66_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1494842.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     IGMAMILFAVTLISLKDLFLEK"
     gene            complement(3606..4541)
                     /locus_tag="VYN66_000782"
     CDS             complement(3606..4541)
                     /locus_tag="VYN66_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV1494843.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4538..5263)
                     /locus_tag="VYN66_000783"
     CDS             complement(4538..5263)
                     /locus_tag="VYN66_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEV1494844.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLCKDRNNVELWFEEV"
     gene            complement(5347..5982)
                     /locus_tag="VYN66_000784"
     CDS             complement(5347..5982)
                     /locus_tag="VYN66_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV1494845.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(5984..6811)
                     /locus_tag="VYN66_000785"
     CDS             complement(5984..6811)
                     /locus_tag="VYN66_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1494846.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6833..7168)
                     /locus_tag="VYN66_000786"
     CDS             complement(6833..7168)
                     /locus_tag="VYN66_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEV1494847.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(7169..7885)
                     /locus_tag="VYN66_000787"
     CDS             complement(7169..7885)
                     /locus_tag="VYN66_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEV1494848.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRAQAPLVIKEVNAIKVALLAYSYGFNGIEQYISQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIVY"
     gene            8398..9009
                     /locus_tag="VYN66_000788"
     CDS             8398..9009
                     /locus_tag="VYN66_000788"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1494849.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9054..9782
                     /gene="rlmB"
                     /locus_tag="VYN66_000789"
     CDS             9054..9782
                     /gene="rlmB"
                     /locus_tag="VYN66_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV1494850.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRTVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9821..10732)
                     /gene="trxB"
                     /locus_tag="VYN66_000790"
     CDS             complement(9821..10732)
                     /gene="trxB"
                     /locus_tag="VYN66_000790"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV1494851.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10798..11022)
                     /locus_tag="VYN66_000791"
     CDS             complement(10798..11022)
                     /locus_tag="VYN66_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV1494852.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11100..11843)
                     /locus_tag="VYN66_000792"
     CDS             complement(11100..11843)
                     /locus_tag="VYN66_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1494853.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11843..12643)
                     /locus_tag="VYN66_000793"
     CDS             complement(11843..12643)
                     /locus_tag="VYN66_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1494854.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12801..13157)
                     /locus_tag="VYN66_000794"
     CDS             complement(12801..13157)
                     /locus_tag="VYN66_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1494855.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13170..13682)
                     /locus_tag="VYN66_000795"
     CDS             complement(13170..13682)
                     /locus_tag="VYN66_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV1494856.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(13682..14176)
                     /locus_tag="VYN66_000796"
     CDS             complement(13682..14176)
                     /locus_tag="VYN66_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1494857.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            14311..14757
                     /locus_tag="VYN66_000797"
     CDS             14311..14757
                     /locus_tag="VYN66_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV1494858.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14754..15401
                     /locus_tag="VYN66_000798"
     CDS             14754..15401
                     /locus_tag="VYN66_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV1494859.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15422..16003)
                     /gene="pdxT"
                     /locus_tag="VYN66_000799"
     CDS             complement(15422..16003)
                     /gene="pdxT"
                     /locus_tag="VYN66_000799"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV1494860.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(16004..16879)
                     /gene="pdxS"
                     /locus_tag="VYN66_000800"
     CDS             complement(16004..16879)
                     /gene="pdxS"
                     /locus_tag="VYN66_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV1494861.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(17031..18410)
                     /locus_tag="VYN66_000801"
     CDS             complement(17031..18410)
                     /locus_tag="VYN66_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1494862.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALAGGKIELFRNGAF
                     LVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18704..19627
                     /locus_tag="VYN66_000802"
     CDS             18704..19627
                     /locus_tag="VYN66_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV1494863.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19687..20292
                     /locus_tag="VYN66_000803"
     CDS             19687..20292
                     /locus_tag="VYN66_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1494864.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20311..21555
                     /locus_tag="VYN66_000804"
     CDS             20311..21555
                     /locus_tag="VYN66_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1494865.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSS
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(21778..22035)
                     /locus_tag="VYN66_000805"
     CDS             complement(21778..22035)
                     /locus_tag="VYN66_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV1494866.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(22077..24113)
                     /locus_tag="VYN66_000806"
     CDS             complement(22077..24113)
                     /locus_tag="VYN66_000806"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV1494867.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAI
                     QTFSTPRRLAVRVTGLADKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGQETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLILSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24373..25290)
                     /gene="glyQ"
                     /locus_tag="VYN66_000807"
     CDS             complement(24373..25290)
                     /gene="glyQ"
                     /locus_tag="VYN66_000807"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV1494868.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            complement(25309..26070)
                     /locus_tag="VYN66_000808"
     CDS             complement(25309..26070)
                     /locus_tag="VYN66_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1494869.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSEQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(26343..27185)
                     /locus_tag="VYN66_000809"
     CDS             complement(26343..27185)
                     /locus_tag="VYN66_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1494870.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLESLKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEEVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTTAVQSGAPRVD
                     DVDF"
     gene            complement(27300..28691)
                     /locus_tag="VYN66_000810"
     CDS             complement(27300..28691)
                     /locus_tag="VYN66_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV1494871.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(28926..29303)
                     /locus_tag="VYN66_000811"
     CDS             complement(28926..29303)
                     /locus_tag="VYN66_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1494872.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            29518..30495
                     /locus_tag="VYN66_000812"
     CDS             29518..30495
                     /locus_tag="VYN66_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1494873.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSNRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            30492..31574
                     /locus_tag="VYN66_000813"
     CDS             30492..31574
                     /locus_tag="VYN66_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1494874.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTINLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(31638..32668)
                     /locus_tag="VYN66_000814"
                     /pseudo
     CDS             complement(31638..32668)
                     /locus_tag="VYN66_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         9771 a         7353 c         5902 g         9898 t
ORIGIN      
        1 ctccatgaat gatagatacc ttgttatgac gcgcttacta tattagaaag ttggctctct
       61 aattaaatta attctcatta tatatatcaa aagaccgcag tttgaaaact acggtctaat
      121 tttttgtcca aatgtaaatc acttggaact attattcaat ttattctttt tagtaaatgt
      181 tcccgtttta atcccagtta cagtttttaa tcctctgtaa ccgttgattt tacaaagctt
      241 ttaatttttt tgcgattact cccactcaac tgttgcaggt ggtttacttg taatatcgta
      301 gacgatacgg ttaacatgat ccacttcatt tacgatacgt actgagattt tttgaagtac
      361 ttcccatgga attttggcaa aatcagcagt cataccatcg atagaagtga tagcacggat
      421 tgcaatcgtg tagtcatacg tacgaccgtc acccataaca ccgactgaac gaacgcctgt
      481 gttaacagtg aagtattgcc aaatatcgcg gtcaagtcca gctttagcga tttcttcacg
      541 aagaatagcg tctgattcac gaacggtttc aagtttctct tcagtgattt cacccatgac
      601 acggatagca agtcctggtc ctgggaatgg ttggcgccat acgatatggt ctggcatacc
      661 aagctctgta ccaagagcac gaacttcatc cttgtaaaga gtattgagtg gttcaatcaa
      721 ttcaaactgc atatcttctg gaagaccacc tacgttgtgg tgtgacttga tagtttgagc
      781 tgtatccgta ccagactcga taacatctgt atataaagta ccttgagcaa ggaatttcac
      841 atctttgagc ttgcttgctt cgtcatcgaa tacatagaca aactcgttac cgatgatttt
      901 acgtttttgt tcaggatcag aaacgccagc aagtttgtca aggaaacgtt tagcagcgtc
      961 tgctttgacg atattcaaac caaacttacc accgagcatg tccataactt gatcagcttc
     1021 gcctttacga agaagaccgt ggtctacgaa gatacagatc aattgatcgc caatcgcttt
     1081 ttggagaaga accccaacga cagatgagtc aacaccacct gatagaccaa gaaggacacg
     1141 tttatcaccg acggtttcac gaattttttt gatctgcatg tcaatgaaat tatccattga
     1201 ccagtcacct ttagccttac aaatgttaag ggcaaagtta cgaaggatat catttccgta
     1261 tacagaatga cgaacttctg ggtggaattg gataccgtaa atgtgtttat ctgggttttc
     1321 gatggctgcg tataggcagt cagctgatgt acctgtacga acaaagtcag caggaatctc
     1381 agtaaccgca tcaccatggc tcatcaaaac agtctgttca tcaggtgttg attcaaaaag
     1441 cgctgatggt gtgtgagtta gggttgattg accgtattca cgatttccag catcacctgc
     1501 aggaacaact tttcctccaa gtttatgggt caataactgc ataccataac agattcccaa
     1561 aattggaatt ccgagttcga agatttctgg gtcaatatca aatgaaccat cttcatatac
     1621 agaatttgga ccacctgata gaataattcc tacaggattg acttcacgaa cttcagcagc
     1681 tgaaatttta tggcttttta gttctgaaaa aacaccaatc tcacggatac ggcgtgaaat
     1741 cagctggttg tactggctac catagtccaa tacgatgatt ttttctacat cttgcaaatc
     1801 agttgaaatg ttgctcatct ttttcctttc tcaaaagaaa tatcttttct caatattcta
     1861 tcacaaataa gggcgaatta ccaactaaac aaggcgaagt ttgacttttt ctagtttatt
     1921 ggggtacaat agagaaaaga tagaaatgaa gtgaaaaata tgctacctgc ttatatgaaa
     1981 atccatgatc agataaaaaa ggatattgac gagcaccgtt gggctattgg tgagaggctt
     2041 cccagtgaaa gagatttagc tgagcagttt gcggtcagtc gcatgaccct ccgccaagcc
     2101 gtatctctat tagtcgaaga aggcgtctta gagcgccgtg taggaagcgg cacctttgtt
     2161 tccagtactc gagtacaaga aaagatgcga gggacaacca gttttactga aattgtcaaa
     2221 tcccaaggta aagttccctc tagccagctc atttcctaca aaaaaaccat tcccaatgag
     2281 caagaagttg ccaagctagg aatttttcca acggacaata ttatccgaat ggagcgggtc
     2341 cgctatgccg accaagttcc cctagtttat gaagttgctt ctattcctga aaaattcatt
     2401 aaggacttta aaaaagaaga aatcaccagt catttcttcc aaaccttgca aaaacatggc
     2461 tatcgtatcg gcaaatctca acagaccatc tatgctcgcc ttgctaaaga aaagattgcc
     2521 cactatttgg aagttgaaaa aggacatgct attcttggat tgacccaggt ttcctaccta
     2581 gaagatggta ctgcttttga atacgtaaaa agtcaatatg taggcgaacg ctttgaattt
     2641 tatcttgaaa ataattagat taccacgcaa aaactctctg tcacggacaa agagtctttt
     2701 ttatttttct aagaataaat ctttcaaaga aatcaaagtt acagcaaaca atatcattgc
     2761 cataccaata aaatcaatgg gataaaattg ttcattcatt attaagaagg caaagaaaac
     2821 cgccgatatt ggctcaattg aagccaacaa gcttgacttg accggtccta tcagactggc
     2881 tcctttaagg aaagctgtat aggcaaagac agtcccgata aggataatgc ctgcaaacgc
     2941 aaggagaaaa tcaagactag tcgggatatc ggcctgtaga acccctgtaa aaggaagggc
     3001 gaccaaacct gctatgacca tccccacacc aatgaccaag ctgctccccc actttttaat
     3061 caaggctatg ggtaaaatga tatacaaagc ataagtcaag gcagaaaaga gaccccagaa
     3121 caggccagca ggtgtcatgg ataactggtc caactgccca tgtgttgcga tcaggaaggt
     3181 tcctccgatg gcgaatatga tggaaactat ctctcccagt gtcggtgcca ccctatcctt
     3241 gatacagcta taaattaaaa ttccgacagg acaaacatac tgaagcaccg tcgctgttcc
     3301 cgcattggtc tcctgaatag cagacagata ggcgaattgg ttgagaaaaa gaccaatcag
     3361 agcaaaaata agaagagaca gcaaactctt tctatccttt aaaaagacca gtattttatc
     3421 ctttgcagta gcataagcca agagcatgag aattccacca gcgattaaaa gacgcaagtt
     3481 ggtcaagacc agagccgaaa ttccgtgtgc cattaggtat tggccactcg ttcctgacaa
     3541 cccccaagca ataccagcca caactgttag taaagtccct tttaaactat ttgacatgtc
     3601 tctccttact cttctttgcg aatcaagttc atcacttgat tgcggtctaa tatccattga
     3661 gctctctcgt ccttttcata ggtacgattc gataggaaaa tagtggcttc ttgcttctga
     3721 cgattccaca tgataaaggt acctgtatag cccgtatggt ctagccaatc tccttccaaa
     3781 ttccatgcta aagaacgttc cttgtcatcc aaaggagaaa aattttgatt taagtctctt
     3841 gcaaaatcat ctgctaaata gtgttctaaa aagatttgta aatcctttat agtcgaaaat
     3901 aaaccagcac tcccagcatg tctacccagg agacgagcct tgggatcatg cactatgcct
     3961 gcctctacac ctctaactgt tggaacagca agctcaactg gcccaaactt ggtttccgtc
     4021 attccccaag gtttccagac ttgatccttt aaaatcacat ccaaatcttg attaaaaatt
     4081 ctttccaaaa taaagcccaa cagcaaaaaa tggacatccg aataaagaaa ggctggctga
     4141 cttcgtctgt tgagatgaaa catcgcttcc tttaattcag gggctgttaa aagatcacga
     4201 ttaggaataa aaggatcaag gtctgttgca tgagtcaaga gctggcgaat agtgatgtct
     4261 ggataatcac tctcaggtaa aaaatctatt accagtctat caatatctaa ttgacctatt
     4321 tcccacaaga aggtacaaac tgtgccaacc ccaacaacct tgctgacact agctaggtca
     4381 taaactagtc ctgcctcagt ctgcaagcca tgctctgggt cactctggcc taaatagaac
     4441 tctgtccatt gattgtcctt aaaatacgca aaagaggctc cgggataaat tcctgcctcg
     4501 atttgttctt ctattttttt aataatcttg gtccacttca tacttcttca aaccacaatt
     4561 caacattatt tctatcttta caaaggaaga atttttcaga cttaggaata aaatattcag
     4621 tagactcaaa cttctggcga agacttgcta tgtctaattc attgaccaag aacttgagca
     4681 tagataagtc ccaggtaacc gtattgtcca cagtcaaatc ctgcccctga gctggaataa
     4741 aatcaaggga tgccccaatt tcagatgatt ccaagaaact ttcgatatca gttgggagat
     4801 gtaattccat agaaatctca aatttactta aagaaattga tgccaaatct gtttgaaatt
     4861 caggcttttc tcctacttct actagacttg ctatgtcatc ttccgcatga atcaaaatca
     4921 aatcatcttc tggtgagaaa atttcaaaag cgtagccatt ttgaccttta tataatcgat
     4981 gaatcgaatc tgttttagat aagattcctt caatttctaa gggattttcc accttgacaa
     5041 tcaatctagc tagttttttt cttccctcta ccttacgagt acgcatactg ggagcttctt
     5101 ctaaaaccag cttttcaaga cccgtttggt cacctagtga cagaaaggcc gattcttcta
     5161 acaaggcctt cattccaagg gtttcaatat aaaatgtttc atttaatttt ctattattag
     5221 cttttaaagt aggaataatc cgtacaatct gatttacatt cataaattcc tccgtcactt
     5281 tttattttaa aggattttag aattttttac aagatattat gctcaaattt aacattattt
     5341 agctatttag taaagatagt ctttcaagga ttttgacaaa gcctgataac cagcaatact
     5401 gaggtgcagt ccatcagttg tatattcttt tttgagttgg cctgcttggt ctgtcaaaca
     5461 atcaaatact ggcacaaatt ccacctgcat ataggcagat gcaagctctt gataggcttg
     5521 attccagttc tgaatttttt cattcgagcg gatatagact gcctgctggt actcctctct
     5581 ctcattgaca ggcaaaatgg aaagcaattt aatctctgtc aatggataat cgcgagcaac
     5641 ggattgaatg atagcttcga gattattgag agcctcattc acaggaacat cctttccgat
     5701 atcatttgtc ccaatcagaa gaaaaatttt atctactgct ccaccatata gatgagcatc
     5761 aaggttctct agtaacagtc ctgtctgata gccacgaatt cctcgattga caatcgtctt
     5821 tgaagtccca aatagctcct gtagaggata atactcgaca atggaatccc caataaaaag
     5881 aatgtttggt tctacaacag aaatgtgatt taggtgacga tacttagttt gaattttttc
     5941 ttgttccttt aggagccaat tttctaataa ctgtactgcc acttcattct cctttttcta
     6001 accagttttc caagacttga taaactcctc cttggctgtt ggctggtgct agagcagtcg
     6061 ccacagcttt ggctttctca tcagcatttt ccatcgcata ggcaattcca gccatttcaa
     6121 gcatttcaac atcattttca ctatcaccaa aagccatgat ttcttgggat ttcaagtccc
     6181 agcgcttgag taattcctcc aagccccatg ctttatgaat cccagcttgg aggatatcaa
     6241 tgcaaccata gccactggat acagctcgga cacggccatc aaagagagca ttgatttctt
     6301 ccaaaaccga actcaaacgt tcctcaccaa caaccatgct catcttgagc acaccaccaa
     6361 agaggtcaga tgttaattca tccacaaatt gcatccgttg gtagaatttt tcaatcattt
     6421 ctggagtcat aaaactttca agatctgtaa aaatcgtacc ttccttgaca aaatcaccct
     6481 tcatccccgt tacaacaaac tggtcctgac acgctcgacc cttgaaatga gtcaaagcct
     6541 tgttgacaat ggcgtcatcc catgtctgag cctgaatcaa ttcattgttt tcaaaaatac
     6601 gagcgccatt agcaacaacc agaaccactc gatccaccaa gggactcagt agttgtctca
     6661 tgcggtgaat ttcattgccc gtcgcaatga caaaacgaat gcccctttga tccaactgat
     6721 ctaaaatctt ttccaatcgt gggagatcaa gctgacctct agcatccagc aaggtcccat
     6781 ccatatctgt cgcaattacc ttaatcatca tttactttcc tctggattca agctaatacc
     6841 acttcaatcc cacatgttca ttcatttctt tataggctgt atcaattcgt tccttagtcg
     6901 cttcatctaa ctggtcacga tgactgccat cctctataaa atcttccaaa atataagttt
     6961 ggtaatgata caagggatat ccttctggtg aaaaggttcc ctttggtgtt cgattgaccc
     7021 aagtaggatg agctttagct gttccgatag ttgtttttcc atccttcttc ttgatggtga
     7081 catccatgag aacaccacgt tcagtccact tagcattctc ttcatctccc atagattcaa
     7141 ttcgttgatt ggaaatgaag ttccccattt aatagacaat gagcttttta tctccatctt
     7201 tttccaccgt ttcagatggt tcaacaacgt gaggatgccc tccaaagata atatccgctc
     7261 cccaatcaat catcttgtga taaagagctt tttgttcttc agttggttcc aatcgatact
     7321 caacacccat ctgaggcatg ataatggtga tatctgcttc cttctctgcc cgttcaactt
     7381 cagcctttat cttatcttcg tttaaatctg aaagataacg attatagtct tcctgagaaa
     7441 tatactgctc aattccattg aaaccatagg aataggctaa caatgcaacc ttgatagcat
     7501 tcacttcctt aatgaccagc ggagcctgag cacgtggttc gtgcgtataa actccgattg
     7561 gagtgattcc agctttctca ataatatcgg ccattgaaat aactccctca atttgcgaat
     7621 ccaaaatatg attatgagct aaatccagca catgataacc tgcctcctta atagcatcca
     7681 taacttcaac aggagcatta aagagaagat aacccgctaa ataatgatcc ttattaatgg
     7741 ttccttcaaa atcaccaata gctaaatctg cttgcttgag ccaaggagtc acatactcaa
     7801 aattttcatg aaagtcatag gtaccgtctt cttttttagc tgaaaagaaa agtccatcgt
     7861 ggtagagcaa atccccgtgg gccataattc tggcagtttt ttcctcttcc tactcctgag
     7921 acttttgctt agtcccctct tgagaaatag tatctcgttt ttgactagtc aagggattcc
     7981 ttggaagctt tcaaacaaca agaccaagcc cattgataaa ccaactgcta gcaagagtat
     8041 cgccacaaat cccttattgc tccacttgcg ataactccta aaaagtttac caagcccttc
     8101 ataaaacgaa aagctgaacc accctgattt cgattttgtc ttctttgtat cttcgttctc
     8161 cctactttct tatgcaagcc ttttcttttt attatatcac agataagtat ttctttcaca
     8221 attgaattga acttcctatc tattttctat aaatcctaaa tgccataatg ctttcaattc
     8281 ttgtcatttt gtgatatcat gtagaggaaa tgaactaatc cacagtggct tattccaagt
     8341 ataccacttg ggctttggca gtagctaact gcactaaaca taatataagg agagaaaatg
     8401 tctgcaatag aacgtattac aaaagctgct cacttaattg atatgaacga tattatccgt
     8461 gaagggaatc ctactctacg cacggttgct gaggaagtca ctttccccct atctgaccag
     8521 gaaatcatcc taggcgaaaa gatgatgcaa ttccttaaac attcccaaga tcctgtcatg
     8581 gctgaaaaaa tgggactccg cggtggtgtt ggactggctg ctccccagtt agatatctca
     8641 aaacgcatta tcgctgtttt ggtacctaat attgttgaag aaggcgaaac tccacaggaa
     8701 gcctacgatt tggaagccat tatgtacaat ccaaaaatcg tctctcactc tgttcaagat
     8761 gctgctcttg gcgaaggaga aggttgcctg tctgttgacc gtaacgtgcc tggctatgtt
     8821 gttcgccatg cccgcgttac tgttgactac tttgacaaag atggagaaaa acaccgtatc
     8881 aaactcaaag gctacaactc cattgttgtt cagcatgaaa ttgaccacat taacggtatc
     8941 atgttttacg atcgcatcaa tgaaaaagac ccatttgcag ttaaagatgg tttactgatt
     9001 cttgaataaa gaaaatcccg ttgcaagacg gggttttgtg ttataataga ggcatgaaaa
     9061 caaatgatat tgtctatggt gtccacgctg ttaccgaagc cctccttgca aatacaggaa
     9121 acaaactcta cctccaagaa gatctccgag gtaagaatgt tgagaaagtc aaggaactag
     9181 ctacagaaaa gaaggtgtcc atttcttgga catcaaaaaa atctctctct gagattactg
     9241 aaggtgctgt tcatcaaggt tttgttctac gagtgtctga atttgcctat agcgagctag
     9301 attacatcct tgcaaaaaca cgccaagaag aaaatccact tctattgatt ctagatggtc
     9361 taaccgatcc ccataatctg ggttctatct tgcgaacagc cgatgcgacc aatgtttcag
     9421 gtgtcatcat tcccaagcac cgtactgtcg gagtaactcc tgtcgttgcc aaaacagcca
     9481 caggtgctat tgaacacgtt ccaattgccc gagtgaccaa cctcagtcaa accttagata
     9541 aacttaagga tgaaggtttc tggacctttg gaacggatat gaacggtact ccttgccaca
     9601 agtggaatac aaaagggaaa atcgccctca tcattggaaa tgaaggaaaa ggtatctcta
     9661 gcaacatcaa aaaacaggtc gatgaaatga ttaccattcc gatgaatgga catgttcaaa
     9721 gccttaatgc cagtgttgct gcggccattc tcatgtacga agttttccga aatagactat
     9781 aaaaaagttt ccagtcatct gattggaaac ttttttatga ttaactatgt tctgtaatga
     9841 atttataggc ttcttgacca gcgatagctc catctccaac cgctgttgtt acttggcgaa
     9901 ggtctttcaa gcgaacatct ccaactgcaa agataccgtc gactgcagtt ttcatgtggt
     9961 tatctgtcac aatccatcct gcctgatctt ggatattcaa ttctttaaca aaatcgctaa
    10021 gagggtccaa accaacatag ataaagacac caccgaaggc ttgttctgtc acttgacctg
    10081 ttttcacatt ttcaaatacg actgattcta ctcggttttc acccttgatt tcccttacta
    10141 cagaatccca gataaagctg attttttcat tcgcaaaggc gcgatcttgt aaaacctttt
    10201 gggcacgaag ttggtcacga cggtgaacaa tggtaacagt cttagcaaaa cgagtcaaga
    10261 agagggcttc ttcaacagct gaatctccac caccaactac caataaatct tggtcacgga
    10321 agaaagcacc atcacacaca gcacagtaag aaacaccacg actgttcagt tcttcttctc
    10381 caggcactcc caaaggacgg tgtttagaac cagttgctac gataactgta cgtgtttcat
    10441 atgtttggtc atcagtcatc actttcttaa aatcaccatg gtcttcgaca ttttcaacat
    10501 aaccataaat gtgctcaaca ccaagatttt caagtggttc aaacatcttt tcagccaatt
    10561 caggtccact aatattagcg tatcctgggt aattttcgat atcagatgta ttattcatct
    10621 gaccacctgg cagaccacct tcaatcaaag ctacttttag attgcttcga gcagcataca
    10681 aggccgcagt catccctgca ggtccagcac cgataataat agtatcgtac atatagattc
    10741 cttctttctt gttgtaacta tctttattct aactctttct tgtcaatcac gcaaggatta
    10801 tgccttgaaa acaagaatta aactcataac cgcaaaggat aatactggga caaccaagac
    10861 tccaatcata atcatatcat agagatgggc ttggcgagcc ctggctgtct tatcaactcc
    10921 cgacatggct ctcagaccaa tccaaatccc taaaaaaatc agaacaagga tggtggtcaa
    10981 gatcaaactc tcgaaatata aagaaaatag ttgcagtagc atgatttctc tcatttctat
    11041 cttttttaaa gagtaaactc agctagtcca actaactgag ttttccttta tctattatat
    11101 caaatataag tccgtttgta actagcgaag aattcttttg tccgctcttc tttaggggtg
    11161 tggataatct catccggagt tccagactcg atgattttcc ccttatctaa gaagagaatt
    11221 ttatccgcaa cttgggctac aaaggacatg tcatgactga ccaaaatcat ggtctgacct
    11281 gacttagcag catctgcaat agacttttct acttcaccga ccaattctgg gtcaagggct
    11341 gaagttggtt cgtctaagag caaaacatct ggtttcatag caagcgcacg cgctagggca
    11401 acccgttgct tctgtccacc tgataaatgg cgaggataat ggttttcacg gtccgaaagc
    11461 ccaaccttag ccaactcttc cttggcaatc ttagtcgctt cttggtcaga taatttcttg
    11521 acaacaacca agccttcttt cacattatca agtgctgttc ggcgttcaaa taaattaaac
    11581 tgttggaaaa ccatagacaa cttacggcgt agggcaagga tttcttcttg agtgatttta
    11641 gaaaaatcaa ctgaaaaacc atcaatctga atagagccac tgtcaggtgt ttcaagataa
    11701 ttgagactgc gaagaaaggt tgattttcca gctcctgaag aaccaatcaa ggctacgact
    11761 tctccttttt gaatatccaa gttcagatga tccaagacag tctgtcctga aaaggatttg
    11821 cttaaattcg aaatcttaat cattaacgaa ggtctccttt cacatctgtt tgcactgtat
    11881 caggtgcaga aatagccatt tttctctcga tgaaacgacc gaggctttca attccgatat
    11941 tgactaccca ataaacaagg gcaacggaga tgaagcgttc aaaatagcga taatcagctc
    12001 cacctagaat ctgagcttgg gcaaagactt ccacaacacc cgcactaaaa gctagagatg
    12061 ttcccttggt caaaccgatg agggaattaa tcaaggttgg agtagctacc accgctgcat
    12121 taggaataat cactcgtcga taaacttgcg ctcgggtcat acccagactg cgtgccgcct
    12181 caatctcacc aggattaact gagagaatgg ctgcacgaat ggtttcacta gcataagctg
    12241 cctcattaaa ggcaaaagcg acaatcgcaa aagctgcagc tggaatcgca ttgatattga
    12301 gaccagttcc ccattgctga ttgagggctt tcaaagccaa agggattccg tagtaggtca
    12361 acatgagttg caccaaaatc ggtgtccctt ttaagaaact aacaaagaag gcctgcaagg
    12421 gatataaaat cttgacacga ttgatcttca caatggcaaa aagaagcgcc aaaaccaagc
    12481 caaaaagggc accaccaatt gtcaacataa ttgttgttgg aagttgttgg acaattctag
    12541 ggattccatc aaagaccgaa cgtaggctaa acagcttgcc atccggaatc aattgcatca
    12601 agttttggta ccaatctgat gctaaaatcg ttgtaacatt cataaaaaca tcctcttctg
    12661 ataatgattc attctaccat gcttctgccg tcaatgaaat tatttgctac ggtcagatac
    12721 agactttgtc tacctactag tttatcatac tttgaaacag ggaagttata tattttatct
    12781 atcaaagaga tagataaaaa ctatttcagt cccagttctt cataagattt tcgataaccg
    12841 atttgcttaa cagttccatt ttctactaaa atgggccgtt ttaacaacat accgtcactt
    12901 gctagcaact cagccgcttc ttggtttgac aaacttccta ccttatcttt tagccctaat
    12961 tcacggtatt tgataccact ggtgttgaaa aattgcttca attcaaatcc tgaggtttct
    13021 agccaattca aaatgacttc ttggctaggt gtttcttcca cgatatggac ggctttatag
    13081 tccacaccta attgatttaa ttcttgtttt gcttttttac aagttgaaca tttggggtat
    13141 tcgataaatt ctaacatgtg cttctccttc tatttgttta tatctttaaa atctacgcct
    13201 tcatgctcca agagccaagc tttcttttcc actcctgcag catagcctgt cagacgcttg
    13261 cctgctccca acacacgatg acaaggtact aggatagacc aaggattgcg tcccactgct
    13321 ccaccaattg cttgagcaga agccacttgc aggtcttgag caatttgtcc ataggtcact
    13381 gtctgaccat aaggaatgcc ctgtaaatag tcccaaactc tcttttcaaa attcgttccg
    13441 attggcgcca agagcaagtt ggataaatcc tgaggcttgc ctttaaagta atcatctaag
    13501 caagcaataa ctgggtctaa aataggatga ctaacaactt cttctatcgt ttcatctcct
    13561 agtcccctct caaaatgctt ctgctcctga acccaaattc catacaaata atggtcatca
    13621 gctacaagtg atagaatacc aattggtgag gagtagagta tttttacata cttcttttgc
    13681 attatttctt taatttttga taggccaagc gttcgccatc tactggcacc ttaccaactt
    13741 gtttaaaacc aagtttttca aaaatatgtt gcataacctt gttttcagca tgcgtatctg
    13801 agcgaaaatc aagataatca aaaccttcaa tcaagccctc taagaaggtt tgagcaactc
    13861 cttttccctg cacatctgct gccacagcaa tacggtgaaa gactagatac tctgactctc
    13921 cagcttgcca gtttccctca taaatagctt cataggcctc ctctggactc ttggtcacag
    13981 cagcataggc tagcagttct ccctcttcca aggctacgta ggcttgccct gagatgatat
    14041 catcgataat aacatctgca tttggatagc cattttgcca ctggtcacta ccagcatctg
    14101 ctaaacattt tttggcatcc tccatcacct gcatgatggc atctacttca tttggaaaag
    14161 ctaaacgaat ttccatcttt tttcctcatt tctgactagt ttctcccatc atagcataat
    14221 ttaagtattt ctacaagcag ttaaaacttg actttgtgtt tttgttattt tataatctag
    14281 ttatcaaaac tatataaaaa ggagttaaaa atgaaaacta ccttttccta cccaaaatgg
    14341 gcagaaattc caaacattga cctctatctg gaccaggttt tgctctatgt caatcaggtc
    14401 tgcgccccta tctctcctaa taaagacaag ggcctaacag catctatggt caacaattat
    14461 gtgaaaaatg gttacctgac aaagcctgac aaaaaaaaat accaacgcca acagattgcc
    14521 cgtttgattg ctatcacaac cctcaagtct gtattttcta ttcaagaaat agcccagaca
    14581 cttaatactc tacaaactca agcaagttca gaccaactct acgatgcttt tgtggactac
    14641 atgaaccaag gaattgaccc agctaaccct attatccaaa ccagctgcca aaccgttaaa
    14701 ctctatcatc aaactctaga cttaatcgat catactcaag aggaggtaat ccaatgaaca
    14761 ctagtcttaa actcagcaaa caactcagtt ttggagagga gattgctaat agcgtgaccc
    14821 atgctgtggg tgcagtcatc atgcttatct tgctgcctat ttcatccatc tatagttatg
    14881 aagcacacgg atttttatcc tctatcggcg tttccatttt cgtcatcagt ctctttctca
    14941 tgttcctatc atccaccatt tatcactcta tggcctatgg ttcgacccac aaatatgttt
    15001 tgcgaatcat tgaccattct atgatttacg ttgccattgc cggatcatac acgcccgttg
    15061 tcttgacctt gatgaataac tggtttggct atctgattat tgtcatccaa tggggaacga
    15121 ccatctttgg tattctctat aaaatctttg ctaaaaaggt caatgagaaa tttagccttg
    15181 ctctttacct gattatgggc tggttggttc tggctatcat tcctgccatt atcagtcaaa
    15241 cgacacccgt tttctggagt ctcatggtaa ctggcggact ctgttataca gttggagctg
    15301 gattttatgc caagaaaaaa ccttatttcc acatgatttg gcatctcttt atcctagctg
    15361 cgtccgcact tcaatacatc gctattgttt attacatgta aaaaagttga gaaattcaat
    15421 ctcaactttt ttctttacac atattgataa agtactggtg caagcgcaca tcatcagtca
    15481 attctggatg aaaagaactt accaacatat ttttttcttg ggctgcaaca atttgattgt
    15541 tcactgttgc taaaatttct acaccctcac caacactact gataatcgga ccacggataa
    15601 aggtcattgg aatcttgcca actcccttac attctgcttc cgtgtagaaa cttcctaatt
    15661 ggcgcccata agcattacgc tcgaccacca tatccatagt tcctagatga ctctctttct
    15721 gagaagtgat ttccttagcc agcaaaatta agcccgcaca ggtcccaaac actggtaagc
    15781 cagatagaat ggcttctcgg atgggaagta gcatgttctg gtcacgtaag agcttgccca
    15841 tggttgtaga ctcaccacca ggcaaaatca aacccgacaa gtcactctga tcttgctgaa
    15901 aatcatctag atttctgagt tctacactct cgacacctaa ttgatctagc acttttgcat
    15961 gttctgcaaa ggccccttgc aaggccaata ttccgatttt catctatttt cctcgttcag
    16021 ccatgagaat ttggatttca ttttcattaa taccaaccat ggcttctcct aaatcttcag
    16081 agatttgagc taggatttga ggattacgga agttagtcac agccttaaca atggcactcg
    16141 ctcgtttaac aggatctcct gacttgaaaa tacctgaacc gacaaagacc ccctctgccc
    16201 ctaattgcat cattaacgca gcatctgctg gcgttgcaac acctccagca gcgaaattta
    16261 caactggcaa ttttccatgt tcatgaacat attggaccaa ttctacaggg acttgcaaat
    16321 ccttggcagc aacataaagc tcgtcctcac gtaagttttg aatgcggcga atttcctgat
    16381 tcatcatacg catatgacga acagcttgga cgatatcccc tgtccctggt tctcctttgg
    16441 tacgaatcat ggaagcacct tcagcgatac gacgcaaggc ttcacccaaa tccttagcac
    16501 cacagacaaa aggaacttgg aattctttct tgtccacatg gaaacggtca tcagctggag
    16561 atagaacttc actctcgtcg atataatcaa tttcaatagc ctctaaaatc tgagcttcaa
    16621 caaaatgccc gattctgacc ttagccatta ctggaatact aaccgcttct tggatttcct
    16681 taatcatctt tgggtcgctc atgcgggaaa ctcctccagc tgcacgaata tcagccggaa
    16741 ttcgttccaa ggccatcaca gctgccgcac cagcagcttc tgcgatacga gcctgttcag
    16801 gattctgcac atccataata acaccaccct tgagcatctg tgccaagttt ttatttagtt
    16861 cataacgatt ttcagtcatt tgttttatcc tcatcatttg tattggtagc taccttttca
    16921 ttttaagtca gaacagaatg aagcacaaga taaaaaaact ataattgtat ggggacaaat
    16981 tagctaaaaa ctatctgacc ttaacttaag gccagatagc tcattcattt ttatttttca
    17041 gccgtaaggg cagccattgt gatgtagttg tatggtttgt tgaagtgtgg caagaagaag
    17101 aggtctgtca atgccaattt atcaattgtc acatgctctt ggatagcaag tgagaacatg
    17161 tggattccca tgctaattgc aatatcatgt gaaaccattt gggcaccaag aatttcacgg
    17221 ctatctttgt caaagacaat cttaattgct acttcatggt tgtcatgttt catgaattct
    17281 ggtttttgaa gatcgttaaa gcctgtttca gttgcgttgt aaccagcagc tttcgctttt
    17341 tcaagagtca aaccagttga aaccatgtga agaccgtaga ttgagatacc atttgaacct
    17401 tgaacaccga ttccttccaa ttcatgtcca caagcattgt aggcaccaac gataccagtg
    17461 cgaacagcat ttgaagcaag agcgatatag cttgtatctt tacgagcatt gtcataaaca
    17521 gtcgcacagt caccaacagc gtaaacgtct gggatagatg tttcttgttt cttgtctaca
    17581 aggaaggcac cgttgcggaa gagttcgatc ttaccacctg caagggctgt gtttggacgg
    17641 aaaccaactg caaggataac catatccacg tcaaagcttt ctttgtcagt aatcaagcgt
    17701 tcaactttac cgtcaccttc gattgcttta acagtttgac ctagagccaa gcggatgttg
    17761 tgatcttcca agttcttcgc catcatttgt gtgaagtctt tgtcatagta accgttcaag
    17821 acagtatcaa cgatatcaac aaggacaact tcttttccaa gacgttcaaa ggcttcagca
    17881 agttcaacac cgatgtaacc accaccaaca acggcgatac ggtcgaggtg ttggctcttg
    17941 tcagaaagtt tattgataac ttcttcagca ttttggtaca atttcacgaa ttgtacgttt
    18001 tcaagagttg ctttaaattc gcggtttcct ttaacaattt caacaccttc gattggtggc
    18061 aagattggtg tagaacctgt agcgaaaatc aatttttcgt atgattcttt gtgctctttt
    18121 ccttcaactt ccgctgtaac tactttgtta tcatagtcga ttgaaagaac aggtgagttc
    18181 atgtaaactt tagcaccttt agcttccaat ttttctttat cagaatagaa caagccttca
    18241 gcaccgtcaa tttgttcacc aatccaaaga gccattccac atcctaggaa agagatgtta
    18301 gagttttggt caaatacaac aatttcgttc tcatttccaa aattatccaa catggtattg
    18361 atacatgctg taccagcgtg gttagcaccg actacaacga ttttactcat aaaaatttcc
    18421 tgccttcatt ctaaatttac cttactagta taccatttac cgttagcgtt tacaaggggg
    18481 gtactattat ttcagcattt tttatcaaaa ataattcccc atttatcctg ttttcaagcc
    18541 tgatgaattt tatgagcata tttcttgact ttttccaaat tttgtttgtg aaaattctaa
    18601 ctttatggta ttataataac atgaaaatga aatgtaaggg cttcaattta gatgattatt
    18661 tgatattaca acttattttc tataagagaa aggagttggt agcttgactc ttcgttcaca
    18721 ttctgaacgg ctaatgggga ctactatcac tatttcatta gtggatgagc aagccgatat
    18781 ctttctccaa aaatcctttg acttgctcaa agaacttgaa taccgcttca acgccaatag
    18841 tcaagaatct gagttgatgg aaatcaatta tcaagcagga atagcccccg tcacggttca
    18901 tccagacctg tttgagctga tttcacttgg gttagagcat agcctagcgc tctctagcca
    18961 cctcaatatc agcattggtc ccttaattca aacctggcgt atcggttttt cagatgccaa
    19021 ggtcgcccag cctcaagaaa ttgaatcggt gctgcctcta atcaatcctc atggtatcga
    19081 gttagattct tctacttcta ctgtgttttt aaaacagaaa ggaatgaaga tcgatctagg
    19141 ttgtttagcc aaaggataca gtgcggataa ggttgcccaa tttcttagga aagagggggt
    19201 gacttctgcc ttgatcaatc tgggagggaa tatcctgacc attggaaaaa atcaggcaag
    19261 aggggataac ccatggcaaa tcgggattca agacccagcc aatcctaggg gaaatcattt
    19321 aatgaccatc cctgttgtca ataaatctgt cgtgacttca ggcatttatg aacgtcacct
    19381 gaccgtcgat ggacaagatt accatcacat ttttgacagt caaacaggat atcctgttga
    19441 aacggaacta gcgagtctaa caatcatctc tgataaatca gtcgatggcg aaatctggac
    19501 gactcgactc tttggagaaa gacctgcttc tatcctctgg caagtcgaaa gtttggaggg
    19561 catcgaagtc atcctcatcg ataaagaagg ccacctaagc tgttcttcag gaattcctac
    19621 tctatagaga caactgtttc aatggaattt taaaattgta ttatgtaaaa agagaaagga
    19681 aatctcatgt taaaacttat tgctattgtt ggaacaaatt caaaacgttc tacaaaccgt
    19741 caattgcttc aatacatgca aaaacacttt actgacaaag ctgaaattga acttgttgaa
    19801 atcaaggcca ttcctgtctt caacaaacca gctgacaagc aagtacctgc tgaaatattg
    19861 gaaattgctg ctaaaatcga agaggcagat ggcgttatta tcggtactcc tgagtatgat
    19921 cactctattc cagctgtttt gatgagcgct cttgcttggt tgtcttatgg tatttaccca
    19981 cttttgaaca aaccaatcat gattacaggt gcttcttacg gtacgcttgg ttcatctcgt
    20041 gcccaattgc aacttcgtca aatcttgaat gctcctgaaa tcaaggcaaa tgttcttcca
    20101 gatgaattct tgctctcaca ctctcttcaa gcatttaacc caagtggcga cttggttgac
    20161 cttgatgtta tcaagaaatt ggatgccatc tttgatgact tccgtatctt tgtaaaaatc
    20221 acagaaaaat tacgtaatgc acaagaatta cttcgcaaag atgctgaaga ctttgactgg
    20281 gaaaatttgt aagataggag accaaaaaga atgaaatttg ttggacttgt tggatcaaac
    20341 tacgatcaat catataaccg taagctcttg gaatttatcc gtcgctactt caaactcaaa
    20401 tttgaattag aagttcttga aattgacgaa gttccaatgt ttaaccaaga tgaaaaatgg
    20461 gatgaaagtt tccaattacg ttatttatat aacaaaatta ctcgtgcaga tggtgttatc
    20521 attgctactc ctgagcataa ccacactatc tcagcttcac tcaaatctgt acttgaatgg
    20581 ctttcatacg aagttcatcc atttgaaaac aagcctgtta tgattgtggg tgcatcatac
    20641 tatgaccaag gaacatcacg tgctcaagtt caccttcgta aaatccttga tgctccaggt
    20701 gttaatgcct acacgcttcc aggtaacgaa ttccttcttg gtaaagctaa ggaagctttt
    20761 gataacaacg gaaatatcac caacgaaggc actgttaaat tccttgaaac ttgcttagat
    20821 aactttgtta aatacgtagg agtcgtttcg aaattgaaaa aaccaaaacc aatcgaatca
    20881 gaagacttgg attgtggaaa accaatcgct acaaccatca cagaagttga ccctgacgat
    20941 ccagaatggg tagaaaaagt tgcagcattc actggtgctg tttctggtga tacctatgtc
    21001 aaattggacc acggtatcct aacagttaac caaattgata tgttcttgaa agctatgcca
    21061 tttgaattga catacgctga cgacaacaac caattcctct actacaacaa cgctcaccaa
    21121 gatccagaca ctatgttcgc taaacgtgta ccacctcaat caggtagccg tatgtcgact
    21181 gttcatggtt ctcttccacc agcacgtatg aagaacgtag agtgggttat cggaacactt
    21241 cgcaacggaa accaagaata cgtccgtacg atcgttccag gttctcctgc aggtgttatc
    21301 aacactcaca actaccaagc aatgtactat cctgatggat catacgctgg tatcaatgaa
    21361 atcgtcttta acttccaacc atggcttgac tggtacctaa aagagactgg tcaacgtttg
    21421 gtgggtggta gcggaccatt tgctcctgct gccggaggtc atggagacgc cgatgctact
    21481 tctagcgctt ctgattcagg ggatgctgga ggccacggtg gtgacgcaga cgctacagcc
    21541 ggtgcaagtt actaatgata gtttttagga actattatgg ttcctttttt agcgaaaaaa
    21601 gactagcaaa cacaaactgc tagtctcttt gttatcgttt catctatctt tgttaaaaga
    21661 aagaatacat atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaggctgta
    21721 cttaagtacg gcaaggtgaa gctgacgtgg tttgaatttg attttcgaag agtattatta
    21781 tgaatttgga tcatcaagac tacggccatg taatcctttt tcacgttgta cttggcgtag
    21841 tttttctggt gtaacatcgt ttccttcttc gtccacaatt ttgattcctt caatgtggtg
    21901 acgaacagcg cggcgataac cttcgatgta ctcctcacgt agtttggctt gttccacttt
    21961 ttcttctggt gttaagcctt ctgttttttt ctttttagca agctcattga tacgagcaat
    22021 ttttttcgga tccatttctt ctcctttgta ataagataaa gtccgtttat caaattttat
    22081 ttagtgttaa tttggttaaa acaagcaaac ttagctgctt tcttggttag ttgtgacaag
    22141 attgccaaac gattttgacg gacagcctga tcttcagcca ttaccatagt attttcaaag
    22201 aaagcatcaa tgactgggct aagcgcaaaa agttgtttca attgctgact tgcaggtcct
    22261 gataaaatga gtgtttctac tgcttctgcc aaagcttttt cttggtcatt ctcaaatagt
    22321 gctgaatcaa ccgtagcaac cccttctgcc ttctcggcca ggttaaaggc acgagaaagt
    22381 gattcaacag atggtttaaa atcttcttcc ttgcttactt ctacgagagc acttgctgct
    22441 tccaacatat ctgccacaac aaagtttgaa cctgcaagaa ctgcttcctt gatatctttt
    22501 ggagtagagc ccatcatctt atcaacacga gccttgataa agtccataac ctctgcttta
    22561 ttttcataag tcaaactgtc aaatttcaat gcataaaggc tatcaatcag ctcatccata
    22621 gcaatgtgcc aaccaaaggc atccaagata cgaaccacac cttgagttgc acgacgaagg
    22681 gcataagggt cattagaacc tgatggaatc aatcctactg agaagaaact caaaatcgta
    22741 tccaatttgt ctgcaatggc tagaactgcg ccgaccttgc tctctggaag ttctccttca
    22801 gctgatgtag gcatgtagtg ttcacgaata gcagctgcca ccgctggagt ttcaccagca
    22861 agaagggtgt atttttcacc cataattcct tggagttcgt caaattcacc aaccatacct
    22921 gtcaacaagt caaacttgta aatggctgct gcacgagcaa ggtcaactgt ttcatccact
    22981 gacaaactag ctttttctgc caaaagtaca gtgatttgac ccgtacgaat catgtgttca
    23041 cgaagggaac caatcttctc atggaaggtg acattgttta atttttcaac aagatctgaa
    23101 atcaccaatt tttggtcttc acgccagaag aattctccgt cttccaagcg ggctaccaag
    23161 actttttcat ttcctttgat gacatttttc aaacgctctg cgtttccgtt acgaacagaa
    23221 atgaagtttg gcaagagttt tccatcttga tcacgaacaa caaagtaacg ctggtgttcc
    23281 ttcatagaag tcaccaagac ttcttctgga acttcaaggt atttagcatc aaaacttccc
    23341 atgaaggcag ttgggtattc aaccaaattc aagacttcat tcagcaaatc cgcatcaatt
    23401 tcgatacgta caccatgttt tgcctcaatt tccttgattt ggtcaacaat catttgctca
    23461 cgttcacatg gatctgcgat tacaaactgc ttacgaaggt cttcttcata gctcaatgct
    23521 gactgaatct tggtttcttg tcccaaaaaa cgatggccac gactcacacg acttcccttg
    23581 atatcaagga aatccaagtc aaactcttgc tcatccaaga gaacagttaa agtgtgaaca
    23641 gggcggatgt attcaaagct atttcccgcc cagtgcatgc tgacagggaa agtcagtgac
    23701 ttcaagacat ccacaatgcc tggaacaatg gcttcaactg cttgaccaat ttcttcctta
    23761 gtgacataga catattcttc acccttgatt tcacggaatt cgatatcttc aacagtcaaa
    23821 cctttcccac ggacaaatcc ttgagctgct ttggtgaagt ttccatcact atctaaggca
    23881 attttctttg ctggaccctt gaaatcttct gttaaatcag actgtttgtc tgcaagacca
    23941 gttacacgaa cagccaaacg acgtggtgtt gagaaagttt gaatggcttc aaaagacagg
    24001 cgttttccct tgaggaaggc tgccattttt tcgcctagtt gtttttcact tggcgtaaca
    24061 acataggctg gtaattcttc aagaccgagt tctactaata agttttttgt catgtttttt
    24121 cctttacatt ttcttcaatc ttttttgata tgcaccttag gtactgggga cgtcgctaac
    24181 taactctgtg agtctgtatg gaaatccaat gctaaattta tcaagatttc caacaatctc
    24241 atcaaagtgg atttagctga ctgatttttg aacctaaggt ttcaaaaatc cccacataac
    24301 agtacagcgg tgcatatccc tctagcaagt cttcgacttg cctctaacga ttttagagta
    24361 cagtattact cactattctg cgtcttctgc taaaagttta actcgtgttt cttcatccaa
    24421 aagtgggtaa cctaggcgtt tgcgttctgc aacaaaggtt ttggctacaa cacgggccaa
    24481 gttacggata cgagcgatat agcctgcacg ctctgttaca gatacggcac cacgcgcgtc
    24541 aagcagatta aaggtatgtg aacatttgag aacatagtca taggcagggt gtaccaagcc
    24601 ttcttctaat gcacgaccag cttctttttc aaacttatca aaattttcaa gcaacatttc
    24661 ttggtccgaa atttcaaatg aatattttga gtgctcatac tcaggctgga taaagatttc
    24721 tccgtatttt acaccatcag cccactcgat atcatagaca gagtctactt cttgaatgta
    24781 agaagccaag cgctccaaac cataggtaac ttccgcagtc acagggctag ttgccaatcc
    24841 accgacttgt tggaaataag tgaactgagt gatttccatt ccgtcaagcc aaacttccca
    24901 accaagacca gctgaaccag ttgatgggtt ttcccagttg tcctcaacaa aacgaatatc
    24961 gtgctccaaa ggattgattc ccaatttttc caaagactca aggtaaagtt cttggatatt
    25021 tgatggagaa ggcttcatga ccacctggaa ttggtggtgt tggtagagac ggttagggtt
    25081 ttccccataa cgaccgtcag caggacgacg tgatggctct acataagctg cattccatgg
    25141 ctcaggtccg atagcacgaa ggaaagtgta aggactcatt gttcccgcac ctttttcatt
    25201 atcataagcc tgcataagca tacaaccttg gtcattccaa aattgttgca aagtaaggat
    25261 gatttcctgg aaagtcaatt tcttagacat tgtttactcc tttagttttt atatttcttt
    25321 ttcaattaat ttctgctgaa caaaccaatc taacaaggga aaagtttttt ctgtatttgc
    25381 ccataaatga taagaatcta agaccgcttg tacactacct aaattttcta ccaacttatc
    25441 tactgtcaaa aagctgcgcc agtattcata aaaaatactt gcatagttct tatcataagt
    25501 cgaagaacca aaatcattca atgaatgcca accatacttc ttctgaaaaa gttctacgag
    25561 agattgatta caaatttttt ccgcttgaaa ttcctcttct gtcaagaaat acttgcgact
    25621 aatatattca accatcccct cttcgaacca gatataagag tcataaccat caaaatcatc
    25681 taaaaaatgc tccgaccaat gagctaactc atgtcctaca atctgtaaga ggaaattttc
    25741 agataaagaa tgatagtgac tttctattgc ttgagtttgc tcagaacact cgtagtccat
    25801 caattgatac aaatacaact ctttccaaac agctaaatca ggcgccataa ccattcgtct
    25861 attatttgta taggctggaa cagcgctttc cctaataatc tgtgtagcag cattaaaatc
    25921 tgaccaaata acggcttgcg gtaaatcaca gactgcaagt tcattcttta aaaaagattg
    25981 ataatctttc aacttttcag tatttcttac tacaaaatca cgaaatgtag ctagttgact
    26041 atcgtctttt acaagataaa ggttgtccac ggctctcctt tctttcacac aatagactga
    26101 ataagcaaaa gaaccacatc tgacaatcct aggttgattt aacctagggg cgtcagaaac
    26161 gcggttccac cctaatttat tacttcattg tttttgaaaa tactacagaa agcgccagct
    26221 cattattttg ccctacttgg ctcccactgt cccaagctcg cttgaagaac gccataagac
    26281 tttctttcct gctgactatt atatcaaaaa ttagaataat gtacaattct ttttatgatt
    26341 ttctagaaat ccacatcatc aactcgtgga gcccccgact gaacagcagt cgtttttaag
    26401 gtttctctct cctcatgact cagttcaatt ccaaaacaat caagattagc ttgaatacga
    26461 gaggttgtga cagattttgg aagtggtaaa aatccttctg ccaagctcca ggccaaggct
    26521 atctgagcaa ccgattttcc gtgatttgct gcgatttctt ggacttgctt gctatcaaac
    26581 agttctcctt gtccaaaagg cccccaagct tccaataaaa ttcccttttc acgacagtaa
    26641 gctacgactt cctcttgata cacacctggc gccaagcgaa cttgattgac cgcaggaacg
    26701 atagttgcag tttcaagcaa ggcatccaaa tgatggggaa gaaaattgct aacgccgata
    26761 gcacggattt tcccttcttg atagaggtct tccatcgctc tccaaacttc cgcattgcga
    26821 gttttccatg cgtcattttc tctgagcggt tttgggttcg gccaatgaat caaatacaaa
    26881 tccaaataat ccaagcccag tttttctata gatttttcca aagcttgacg agtttgctca
    26941 taggtttgct gactattcca aagcttggta gttacgaaca tttcttcacg tggaactccg
    27001 ctatctttga ttgcttgacc aacactttct tcattctgat aaatcgccgc cgtatcaata
    27061 tgacgataac cagccttcaa ggattctaac actgcacgat aggcttcttc tccatcctta
    27121 gccttaaaag ttccaaatcc caatactgga atttctactc cattatttaa ttgataggta
    27181 ttcatatgct tctccttcta tatttattca tcaattatag cataaaacat tttttaagta
    27241 gtactatctt ctgtaaaaaa taagtctgtc aaatatctaa cagacctatt ttttcttgct
    27301 tattcatcac gactatagta cagctcatga gcttttaggc gagtattgag catctctggt
    27361 atggtcacaa aggtataacc ttgatttttc aaatactcaa tgacccttgg caaggcattg
    27421 actgtcggac tgtgaatatc atgcatcaaa acgatagagc cgttagctac ttgatgctga
    27481 atttctgtca aaatagatgc ttcattttta ctcttccagt ccagactatc cacatcccac
    27541 atgataaagc tcaaatccaa gctattgcga atatcatctg taatagcacc ataaggtgga
    27601 cgcatgagtt tagaactaga acccagcact ttagttagca catcctcagt atcagtaatc
    27661 tgctttttag cttcatcaag agagagttgc gagagaatcg gatggctcca actatggttt
    27721 ccaacaacat gaccttcaga ttttatcctc ttcaccaagt cctcattccc agaaacattt
    27781 ttcccaagca caaagaaagt cgctttaata tcatatttag ctagggtctc taatacctgc
    27841 ggggtcgttg ctggatttgg accatcatca aaggttagag cgacaacttt ttgatatttc
    27901 ttatcaaagt aagattggta caaggccgca tctttttcga gtaagtacga agattggata
    27961 acatcaaaga aagcagatac tggcaaggct atctcttcta aattttcaac cacaggactt
    28021 ggataaagga taatctgact atccttgtaa tcaaaattcc atgcagacaa gtcttggtca
    28081 gagaagtttt ttacaatctg ctcactctgg tcttgctcta tttttttatc ctctatgaag
    28141 gaggtcaact cttttatcag ctgttcctta gccttactag catctgaaaa cagttggtca
    28201 agtgtaaaag gttgcccatc ttctgttaag tgaacctttc ctagactggt cttttcagtc
    28261 tcttcaacct taaaagcaac taaatcatag atttgtttgg tcacattacg attaacgact
    28321 ccctttaaac ctgactcttc ttgctctgta tagtagaaaa caagattgtc cttactttct
    28381 agcttgtcct taacatcttg atttatcaac tccctaacag aggaaatcac tttctcccct
    28441 tggagaggat aataggcaat cacttcggct tgcccctgac gaaaatgctc cttctgattt
    28501 ccctcactca attgatcatc tttctctttt ttgagcgatt caatcttttg ctcaaaactt
    28561 ttttgctggt agatcttaaa agcaataaat ccgcctaata ggcaaataga aattgctaaa
    28621 ataccaatca aagccaataa tacatgtctc gttttcccgt gtctgccacg tcctagtcta
    28681 cttttattca tatttatatc ataacaaatt tgagcaataa tttcaatgat acaatttggc
    28741 aatccacaat tcctctagca agccaaagca tcttacatca actccaacat ccgtatctgt
    28801 agagattatg tagttttcta tctatggaat ttctagtaga ttgaagctag aatagtagac
    28861 ctatacttct aaaacattgt tagaaatcga tttgattgtc ctgatcgatt tgtcctgttc
    28921 tcatttcatt ttactataaa actatgaaaa gctaggtcat ctgagaagta attactatcg
    28981 acaattgcga tagcgtatac tggtgcgatt tgtttgtagc tctggtgggt atcaccttca
    29041 cgttggcgaa ttttttctag gttttgatta acctgactgc acagataagc ccataggcga
    29101 ttgatgaaaa aattctgatg atgaacctga atttcgatga taacttggat tccattatct
    29161 aactccgcca agacgtctat actagtatag aaatcctgcg ctgagtaggg catggaaggc
    29221 aagacgtgaa tgttacttcc ctccaaaatc gtcacatttt ttgctggcaa gtccagcata
    29281 tcgcggataa attgacaaat cattagttgg gctgataccc ggatgtctga gaatcctcct
    29341 tttcctcctt ctattatagc acatttttaa atctaggttt gctagattct atgcttctat
    29401 ctatttattc ggaaaagaaa gctaatagct tatttgaaat tttctcctct ctgttggata
    29461 gtcccgtcta tctatgttat aatgaaagaa ggatttaaaa tcggaaaagg agtatttatg
    29521 ctaaaattag gtgtcatcgg aactggcgct atcagccatc atttcataga agcagcccat
    29581 accagtggag aataccaact ggtcgcaatc tattctagaa aactagaaac tgcagcaacc
    29641 tttgcttctc gctatcagaa tatccaacta ttcgatcaat tagaagtctt cttcaaatct
    29701 tcttttgatt tagtctatat cgccagtcca aactccttac attttgctca ggcaaaagct
    29761 gccttgtctg ctggtaaaca tgttattctc gaaaagccag ctgtcactca accacaagaa
    29821 tggtttgatt tgattcaaac agctgaaaaa aataactgtt ttatctttga agcagctcgt
    29881 aattaccacg agaaagcctt tactactatc aaaaactttt tagcagataa gcaagtgtta
    29941 ggagcagatt tcaactatgc caaatactct tccaagatgc ctgacttgtt gactggacaa
    30001 acaccaaatg tcttttcaaa ccgttttgct ggtggagccc tcatggattt ggggatttat
    30061 cccctctatg ctgctgttcg tctttttgga aaagccaatg acgcgactta ccatgctcaa
    30121 cagcttgaca atagcattga cctaaatgga gatggtatcc tcttctaccc agactatcaa
    30181 gttcacatca aggctggaaa aaacatcact tccaatcttc cttgcgagat ttatacaaca
    30241 gatggaacct tgactctcaa cacgattgag catattcgct cagctatttt taccgaccac
    30301 caaggaaatc aagtccagct tcctatccaa caggctcctc atacgatgac tgaggaagtc
    30361 gctgcatttg cacacatgat ccagcaacca gatctgaatc tctaccaaac ttggctgtat
    30421 gatgcaggct ctgttcatga gctactatat accatgcgcc agactgctgg cattagattt
    30481 gaggcagaaa aatgaaaacc aaactaccga ctgaatggca agaactgagt gaccaactcg
    30541 gtttccaaga attcaccccc attcaaactc aactatttga gccccttctt gctggagaaa
    30601 acctcctagg agtgagccaa accggaactg gtaagaccct agcgtatctc ctaccaagtc
    30661 ttctcagact acaaaagaaa aaagcccaac aactcttgat tctagcacca aacacggaac
    30721 ttgctggaca gatttttgat gtatgtaaaa cgtgggcaga agctatcgga ttgactgctc
    30781 aactcttcct ttcaggttca agtcagaaac gacaaattga acgccttaaa aaggggccag
    30841 aaattctgat tggaactcct ggccgtatct ttgaactcat taaacttaaa aaaatcaaga
    30901 tgatgaatgt ggaaaccatc atcctggatg aatttgacca attactagat gattctcaga
    30961 ttcactttgt agaaaaaatc actcactacg cacctcgtga ccaccaactg gtctacatga
    31021 gtgccacgac caagtttgac caagaaaaga ttgtgccgaa tacacgcact attaatctct
    31081 ctgaccaaaa attggacaat atccagcatt tctacatgca ggtagaccaa cgtcatcgag
    31141 tggatatgct acgaaaactg gctcatgtag aggatttccg tggtctagtc ttcttcaata
    31201 gcctgtcaga ccttgggaat gccgaggaaa aactacagta tcgtgatata ttgactgttt
    31261 ccctcgctag tgatgttaat gttaaattta gaaaaatcat cttagaaaag tttaaagata
    31321 accaactaac cctgcttctt gcaactgacc ttttggctcg tggaattgat atcgatagcc
    31381 tagaatgtgt cgtaaacttt gatattccta gagatagcga aacctacaca caccgtgctg
    31441 gccgtactgg ccgtatgggc aaggaaggat atgttattac tctcgtcact catccagaag
    31501 aaatcaaaaa acttaaaaag tttgcaagta tccgagaaat tgtcctaaaa aaccaagaac
    31561 tctatatcaa ataaagcccc attgctctat acacatccag tgtatggagc ttttttataa
    31621 gcaattcaat tttatactca tctgctttca aaaagcattc tattccatct ccgattaacg
    31681 atggacttta tcacctcctt ctccagtcct tgtatgacat cttggagttg attcatgaca
    31741 tcttccaaag ttcgaaaggc tttattctta aatccacgtt tacgaatctc tttccacact
    31801 tgttcaatgg gttcatctct ggtgtgtatg gaggaataaa ggtaaaatca atattagtcg
    31861 gaatctttaa ggtacttgat ttatgccata tagcattgtc cataacgagt aaaaaataat
    31921 catctggata agcttgtgaa agctcttcta aaaagacgtt catccactca gtattacatc
    31981 caccagctat taagaaaaat gattcgcctg tatggggcat caacagctcc ataacaatag
    32041 cgaaattctc gtatatagtg actatggaca tgtggaccta ctcctattgg agaccaacaa
    32101 gatcccagtt tactgattct accgaaacca gcctcatctt ggtacatcaa gcgaacctta
    32161 tgaaaaggtc tactggtttt aaagcgcttt cttgtcttct tgaattgaga ttttattttt
    32221 agacgcgaca atggtttgag cgtctgcttt cctaggatgt tctggacgtg gcataatatt
    32281 tcgccaacca tggtgcttca acagttgata gaaggcatca cgtgtgtagg aacgatataa
    32341 gcctgaaata aggtattgta acaaattctc ctgcctctgt agccttcaaa tggcgggcaa
    32401 gaaaggcttt ctcttcctca actgtcatat atgcatggtt acgaccacca cgtgtttctt
    32461 gaaggagaga gtcgagtccg aactcctcat atttttttac atttcgccaa atcgttgttt
    32521 gattacagtc taaaagatct ataatctctt tataagattt gcccatcaga cgaaatagaa
    32581 cgatttgaag gcgtttatga tatttagctg tacgaaagtc ttttaaaagt gttttgatgg
    32641 tttggatttc ttctttagtt gatttcatat tactattgta taatgctttt tgatttgagt
    32701 ctagtattat actcttcgaa aatctcttca aaccgcgtca gcttcgcctt gtcgtataga
    32761 atgttactga cttcgtcagt tctatctaca acctaaaaac agtgttttga gcagtctgca
    32821 gctaactttc tataacaaaa tgaaataaga acaagacaaa tcaatcaaga caatcaaatc
    32881 gatttctaac aatgttttag aagtagatgt gtactattcc agtt
//
LOCUS       DAVLVF010000019        29764 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVLVF010000019 DAVLVF010000000
VERSION     DAVLVF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29764
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.019"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            354..1517
                     /locus_tag="VYN66_000815"
     CDS             354..1517
                     /locus_tag="VYN66_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV1494875.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1514..2191
                     /locus_tag="VYN66_000816"
     CDS             1514..2191
                     /locus_tag="VYN66_000816"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV1494876.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2181..3035
                     /locus_tag="VYN66_000817"
     CDS             2181..3035
                     /locus_tag="VYN66_000817"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV1494877.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALENVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3054..4121
                     /locus_tag="VYN66_000818"
     CDS             3054..4121
                     /locus_tag="VYN66_000818"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV1494878.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTELNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKTRGNTLKLVLVS
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            4131..5297
                     /gene="aroC"
                     /locus_tag="VYN66_000819"
     CDS             4131..5297
                     /gene="aroC"
                     /locus_tag="VYN66_000819"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV1494879.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5307..6410
                     /locus_tag="VYN66_000820"
     CDS             5307..6410
                     /locus_tag="VYN66_000820"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV1494880.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6421..6759
                     /locus_tag="VYN66_000821"
     CDS             6421..6759
                     /locus_tag="VYN66_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV1494881.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAHTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6851..8134
                     /gene="aroA"
                     /locus_tag="VYN66_000822"
     CDS             6851..8134
                     /gene="aroA"
                     /locus_tag="VYN66_000822"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV1494882.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8127..8603
                     /locus_tag="VYN66_000823"
     CDS             8127..8603
                     /locus_tag="VYN66_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV1494883.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8600..9448
                     /gene="pheA"
                     /locus_tag="VYN66_000824"
     CDS             8600..9448
                     /gene="pheA"
                     /locus_tag="VYN66_000824"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1494884.1"
                     /translation="MKIAYLGPKGSFSHHVVQIAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9445..10719
                     /gene="psr"
                     /locus_tag="VYN66_000825"
     CDS             9445..10719
                     /gene="psr"
                     /locus_tag="VYN66_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV1494885.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            10998..11843
                     /locus_tag="VYN66_000826"
     CDS             10998..11843
                     /locus_tag="VYN66_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1494886.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLCYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11833..12936
                     /locus_tag="VYN66_000827"
     CDS             11833..12936
                     /locus_tag="VYN66_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1494887.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTNVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12938..13924
                     /locus_tag="VYN66_000828"
     CDS             12938..13924
                     /locus_tag="VYN66_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1494888.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13940..15373
                     /locus_tag="VYN66_000829"
     CDS             13940..15373
                     /locus_tag="VYN66_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV1494889.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15401..16435
                     /locus_tag="VYN66_000830"
     CDS             15401..16435
                     /locus_tag="VYN66_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1494890.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16635..17372
                     /gene="mecA"
                     /locus_tag="VYN66_000831"
     CDS             16635..17372
                     /gene="mecA"
                     /locus_tag="VYN66_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV1494891.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17523..18809
                     /locus_tag="VYN66_000832"
     CDS             17523..18809
                     /locus_tag="VYN66_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV1494892.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQEFGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18811..19680
                     /locus_tag="VYN66_000833"
     CDS             18811..19680
                     /locus_tag="VYN66_000833"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV1494893.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19756..19830
                     /locus_tag="VYN66_000834"
                     /pseudo
     CDS             19756..19830
                     /locus_tag="VYN66_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            20020..20958
                     /gene="msrB"
                     /locus_tag="VYN66_000835"
     CDS             20020..20958
                     /gene="msrB"
                     /locus_tag="VYN66_000835"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1494894.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            21016..22740
                     /locus_tag="VYN66_000836"
     CDS             21016..22740
                     /locus_tag="VYN66_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494895.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPIDKGSIDL
                     YRDGSSPRNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22742..24490
                     /locus_tag="VYN66_000837"
     CDS             22742..24490
                     /locus_tag="VYN66_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494896.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGSA
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDTFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24837..26096
                     /locus_tag="VYN66_000838"
     CDS             24837..26096
                     /locus_tag="VYN66_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV1494897.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26172..26307
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26346..26846
                     /locus_tag="VYN66_000839"
     CDS             26346..26846
                     /locus_tag="VYN66_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV1494898.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26922..27290
                     /gene="rplL"
                     /locus_tag="VYN66_000840"
     CDS             26922..27290
                     /gene="rplL"
                     /locus_tag="VYN66_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV1494899.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27477..27647
                     /locus_tag="VYN66_000841"
     CDS             27477..27647
                     /locus_tag="VYN66_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494900.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            27647..27895
                     /locus_tag="VYN66_000842"
                     /pseudo
     CDS             27647..27895
                     /locus_tag="VYN66_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            27909..28249
                     /locus_tag="VYN66_000843"
                     /pseudo
     CDS             27909..28249
                     /locus_tag="VYN66_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28242..28622
                     /locus_tag="VYN66_000844"
     CDS             28242..28622
                     /locus_tag="VYN66_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1494901.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            28635..28778
                     /locus_tag="VYN66_000845"
     CDS             28635..28778
                     /locus_tag="VYN66_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494902.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            28871..29458
                     /locus_tag="VYN66_000846"
     CDS             28871..29458
                     /locus_tag="VYN66_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1494903.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            29660..>29764
                     /locus_tag="VYN66_000847"
     CDS             29660..>29764
                     /locus_tag="VYN66_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEV1494904.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCG"
BASE COUNT         8856 a         5462 c         6673 g         8773 t
ORIGIN      
        1 aaaaggcttc tctataaaat caaattttcc agatacctac tgaagctgat ttatttcttc
       61 atctttcttc tactaccttt gtcactaaat ttgacagaca catattgctg atacaacctc
      121 tctacctata atctcacggt taacctgagg ctagtttcat agtttgctat ttgattttcg
      181 ttgataaaaa gaaatgctcc tccaagacgg aagagcattt tttattgtga ttcttacaca
      241 tttatttcct agttttgcag gaagtctagt ataccatttt ttaaaagaag agtaaaaaag
      301 caagggtagt ttcttttttg agaattgtct aaaagtttga tataatggta gttatgaata
      361 gaataagagt cagcaaaagg gttgaaaaga agcttgctaa ggggctagtt ttactagaag
      421 ccagtgatct tgagaatgtc aatcttaagg atcaggaagt agaggtgcag ggtcaggaag
      481 gaaactttct tgggactgcc tacctttctc agcaaaacaa gggcttgggc tggtttgtca
      541 gcaaagacaa ggtggccttc aatcaaactt tctttgaaac gttgtttaga aaagccaaag
      601 aaaagagaaa cgcctactat caagatgatt tgacaactgc ctttcgtctc tttaatcaag
      661 agggagatgg ctttgggggt ctgacagtgg acctttatgg cgactacgcc gtcttttctt
      721 ggtataactc ttatgtttat cagattcgtc agactatatc agaagccttt agacaggttt
      781 tccctgaggt tttaggggct tatgagaaaa tccgctttaa gggttttgac tatgaatctg
      841 cccatgttta tggtcaagaa gcacctgact ttttcactgt tttagaaaat ggtgtcctgt
      901 atcaagtctt tatgaatgat ggcttgatga caggaatttt cctagaccag catgaggttc
      961 gcggtagttt agttgacggc ttggctatgg gtaaatcctt gctcaatatg ttttcctaca
     1021 cagcggcttt ttcagtagct gcggccatgg gaggagctag ccagacaact tctgttgatc
     1081 tagccaaacg ttcacgagaa ttgtctcaag cgcattttca ggcaaatggg ctcagcacag
     1141 acgagcatcg ttttatagtc atggatgtct ttgagtattt caaatatgcc aaacgcaaag
     1201 acttgaccta cgatgtgatt gtcctagatc cgcctagctt tgctcggaat aaaaaacaaa
     1261 ctttctctgt ggccaaggat tatcacaagt tgatttccca gagtcttgag attttaaatc
     1321 cgggagggat tatcattgcc agtaccaatg ctgccaatgt ttcccgtcag aaatttacag
     1381 aacaaattga taaaggcttt gcaggaagaa gttaccagat tttaaacaaa tatggtcttc
     1441 cagcagattt tgcctataat aaaaaagatg aaagtagtaa ttacctcaag gtgattagta
     1501 tgaaggttag taaatgaaat taatcgtttc agtaatgcca agaagtttag aggaggctca
     1561 ggctctggat gccacgaggt acctggatgc cgacatcatt gaatggcgtg ccgactatct
     1621 gcctaaagaa gcgattttgc aggtagctcc agccattttt gaaaaattcg caggccgtga
     1681 gttggttttc acgctacgaa ctcgctccga aggtggagaa atcgaccttt ctccagaaga
     1741 gtatatccat ctaatcaagg aagttgcgca attctatcaa ccagactata ttgattttga
     1801 gtactatagc tacaaggatg tttttgagga aatgctggac ttcccaaatc ttgttttgag
     1861 ttaccataat ttccaagaaa cacctgagaa catgatggaa atcttgtcag agttgacgat
     1921 cctaaatcca aaacttgtta aggttgcggt gatggctcac acggagcagg atgtcttaga
     1981 cttgatgaac tatacacgag gctttaaaac cctcaatcct gaacaggaat atgtgaccat
     2041 ttctatgggt aaggtgggca aggtctctcg tatcactgcg gatgtgactg gttcgagttg
     2101 gtcttttgcc agtctggatg aggttagtgc ccctgggcag atttctctag ctagcatgaa
     2161 aaaaatcagg gagattttgg atgaagcttg atggctatac acgtttagct gcagttgttg
     2221 ccaatcctat taagcattct atttctccct tcatccacaa tagagccttt gaggcgacag
     2281 ctaccaacgg tgcttatgtg gcttgggaga ttgaagcgag tgacttggca gaaacagtgg
     2341 ccaatattcg tcgctaccag atgtttggca tcaatctgtc catgccctat aaggagcagg
     2401 tgattcctta tttggataag ctgagcgatg aagcgcgctt gattggtgcg gttaatacgg
     2461 ttgtcaatga gaatggcaat ttaattggat ataatacaga tggcaaggga ttttttaagt
     2521 gcttgccttc ttttacaatt tcaggtaaaa agatgaccct gctgggtgca ggtggtgcgg
     2581 ctaaatcaat cttggcacag gctattttgg atggcgtcag tcagatttcg gtctttgttc
     2641 gttccgtttc tatggaaaaa acaagacctt acctagacaa gttacaggag cagacaggtt
     2701 ttaaagtgga tttgtgtgct ttagagaatg tttctgaact gcaagcaagg attgccgagt
     2761 cggatttgct agttaatgcc accagtgtgg gcatggatgg ccaatcctct cctgttcctg
     2821 aaaacatagt cttaccagaa actctcttag ttgcagatat catataccaa ccctttgaaa
     2881 caccgttttt gaaatgggct agaagtcagg gcaatccagc agtcaatggt ctgggaatgt
     2941 tgctctatca agctgcagaa gcttttcaac tgtggacagg caaggaaatg ccgacagaag
     3001 agatttggca gtctttaaca gaaaaatacc aataaagaaa gggagactct cctatgaaaa
     3061 tcagaatcga tattcctcat catccttatg atattcagat tgaaaaaggt tgtatggccc
     3121 aggctggtca gtggttgcga gaactctggc aaccgcaaaa ggtagtcatt gtgacagata
     3181 accatgtagc ctctctctat gcagagaagg tcaagctcag cctagaagat gctggttttc
     3241 aggtagctgt ttttgatttc ttagaaggtg aagaaagaaa gaatttaact actgttcaga
     3301 aagtctatga atttctagtc aagcaaggtc tgactcgtag cgatggaatc gttgctcttg
     3361 gtggtggcgt tgttggggac ctggctggat ttgtagcctc tacctatatg cggggtattc
     3421 actttgttca gattccgact agtttgacag ctcaggttga ttcttctatc ggtggaaaga
     3481 caggcgttaa cacaccattt gctaaaaata tggtggggac ctttgcccaa ccagatgggg
     3541 ttctgattga tccacttgtt cttgaaaccc tcggaaaaag agagttgatt gaagggatgg
     3601 gtgaagttat caagtatggc ttgattgagg atccagaact gtgggctctc ttgacggaac
     3661 tgaatggttc tgttgagagc attttggaac atgcagagac cttgattgaa cattcttgtc
     3721 aggtgaagcg caagatggtg gttgaagatg agttggacaa tggtattcgt ctttacctca
     3781 attttggcca cactattggc catgccatcg aagcgactgc cggttatggc aaggtcatgc
     3841 atggagaggc tgttgccatg ggaatggtac agatttccaa ggttgctgag gaaaaaggcc
     3901 tcatgccagc tggcattacc caatctatca cagagatgtg tcagaaattc ggcttgcctg
     3961 ttgactatga aaattgggaa gttgacaagc tttatcaggc tcttactcat gacaagaaaa
     4021 cgcgtggtaa caccttgaaa ttggtcttgg tgtcagagct tggttcagcg atcattcacc
     4081 cagtttctct ggaagagatg aaagactact tggtaaaata aggagagtgt atgagatatt
     4141 taactgcagg agaatcacac ggcccccgtc taacagctat tattgaggga attccagctg
     4201 gacttccatt gacagctgag gatatcaatg aggaccttag acgccgtcag ggtggctacg
     4261 gtcgtggtgg tcgtatgaag attgagagtg accaggttgt ctttacttcg ggcgttcgcc
     4321 acgggaagac gacaggggcg cctattacta tggatgtcat caataaggac caccagaaat
     4381 ggctggacat catgtctgcg gaggacattg aagaccgcct taaaagcaag cggaaaatta
     4441 ctcatcctcg cccaggtcat gccgatttgg ttggggggat taagtaccgt tttgatgatt
     4501 tgcgaaattc tttggagcgt tcatcagctc gtgaaaccac catgcgggtg gcagttggtg
     4561 cagtagccaa acgcctcttg gctgagctgg atatggagat tgccaaccat gtcgtggtct
     4621 ttggtggcaa ggaaatcgat gttcctgaaa atctgacagt cgctgaaatt aagcaaagag
     4681 ctgcccagtc tgaagtttct attgttaacc aagaacgaga acaggaaatc aaggactata
     4741 ttgaccaaat caaacgtgat ggtgatacca tcggtggggt tgtggagaca gtcgtcggag
     4801 gcgttccagt tggtcttggt tcctatgtcc aatgggatag aaaattggat gcaagattgg
     4861 ctcaagctgt tgtctctatc aatgccttta aaggggtgga atttggtctt ggctttgagg
     4921 ctggttatcg taaaggcagc caagttatgg atgaaattct ctggtctaaa gaagacggtt
     4981 atactcgccg taccaataat ctaggtggtt ttgaaggtgg tatgactaat gggcaaccca
     5041 tcgttgttcg tggggtcatg aaacccattc ctactcttta taaacctctt atgagtgtgg
     5101 atatcgaaac ccacgaacct tacaaggcaa ccgtggagag aagtgatccg actgctcttc
     5161 cagctgcagg aatggtcatg gaagcagttg tagcaacggt tctggcgcaa gaaatcctcg
     5221 aaaaattctc atcagataat cttgaggaac taaaagaagc ggtagccaaa caccgagact
     5281 atacaaagaa ctattaagga gttcctatgg caaaaacaat ctatatcgca ggtcttgggt
     5341 tgattggagc ctctatggca cttggtatca aacgcgatca tccagattat gaaattttag
     5401 gttataatcg tagtcaagct tcgagagata tcgccttgaa agaaggcatg attgaccgtg
     5461 caacggatga ttttgctagt tttgctcctt tggcagatgt cattatcctc agcttgccaa
     5521 tcaaacaaac tattgctttc attaaggagt tggccaattt ggatttgcga gaaggcgtta
     5581 ttatttcaga tgctggttcg accaagtcaa ccattgtgga tgcggcggag cagtatttgg
     5641 ctggcaagtc tgttcgcttt gtcggggccc atcccatggc tggtagtcac aagacagggg
     5701 ctgcttcggc agatgtcaat ctttttgaaa atgcctatta tatctttaca ccttcaagcc
     5761 tgacaagtca ggacacgctt aaggaaatga aggatctgct ttcaggtctt catgctcgtt
     5821 ttatcgagat tgatgccaag gagcatgatc gtgtcacttc tcagattagc cattttcctc
     5881 atattttggc ttctagtctc atggagcaga ctgcggtcta tgctcaagag catgagatgg
     5941 caaggcgctt tgcggcaggt ggttttcgag atatgacccg aattgcggaa agcgagccag
     6001 gaatgtggac ctccattctc ttgtccaata gcgagaccat tctggataga attgaggatt
     6061 tcaaggaacg tttggaagtg attggtcagg ccattagtaa gggagatgaa gagcaaattt
     6121 ggaacttttt taaccaagcg cgtgaacaac gccagaccat ggaaatccat aagcgtggtg
     6181 gtgtggatag ctcttacgac ctctatgttg acgttcctga tgaagaagat gtcatcctgc
     6241 ggattttgga actgctacgt gggacttcct tggttaacat ccacatcaac gaggaaaatc
     6301 gtgaggatat tcacggaatc ctacaaattt catttaaaaa tgctcaagac ttggaaagag
     6361 ctgagcatct cataacagaa aataccgact atacagtcgt catcaaataa ggagaagaac
     6421 atgtcaaata tttacgatag tgcaaacgaa ctcagtcgcg gtctacgcgg attaccagaa
     6481 tacaaggctg ttaaagcagc taaagatgcg attgcagcag atgctgaggc aagcaaaatc
     6541 tttacagaat atcttgcctt tcaagaggaa attcaaaaac tagcacatac aggtcaaatg
     6601 ccagatgcgt ccttccaagc taagatggaa ggctttggta aacagattca aggaaatagc
     6661 ctcttgtcag aattctttac caagcaacaa caattggcca tttacctttc tgacattgaa
     6721 aaaattgttt ttgaaccagt ttcagaattg ctgaaataag aaaagaacga tcctaaagtc
     6781 aggaattttt aaggaatttc tggcttttta tgataaaata ggtaaaagta ttgcaagtat
     6841 gaggtccagt atgaaactaa aaacaaacat tcgccattta catggtagta tccgcgtccc
     6901 aggtgacaag tctatcagcc accgttccat tatctttgga agtttggctg agggtgagac
     6961 caaggtttat gatattctgc gaggtgaaga cgttctttcg accatgcagg tttttcgtga
     7021 ccttggtgtt gaaattgagg ataaagatgg ggttattacc attcaaggtg taggcatggc
     7081 tggcttaaaa gcgccacaaa atgcccttaa tatgggaaat tctggcacct cgattcgcct
     7141 gatttcaggt gtccttgctg gtgcagattt cgaagtagag atgtttggag atgatagtct
     7201 ttccaaacgt cctatggacc gtgtgaccct tccactgaaa aaaatgggcg tcagcatttc
     7261 agggcaaact gaacgagact tgcctcccct tcgcttaaaa gggacgaaaa acctaagacc
     7321 tattcattat gagttgccaa ttgcctctgc ccaagtcaag tcagccttga tgtttgcagc
     7381 cttacaagct aagggggagt cagttattat cgaaaaagag tgcacccgta atcatactga
     7441 agatatgttg aaacaatttg gtggtcattt aagtgtggac ggtaagaaaa tcacagtcca
     7501 agggccacaa aaattgacag gacagaaggt ggtcgtacca ggagatattt ccagtgcagc
     7561 cttttggtta gtcgcaggtt tgattgttcc aaattctcgt ctagtgctgc agaatgtggg
     7621 gatcaacgaa actcgcaccg gtattattga tgtcattcgt gccatgggtg gaaaattgga
     7681 aataactgaa atcgatccag tcgctaaatc ttctaccttg actgttgagt cttctgactt
     7741 gaaaggaaca gagattggtg gcgctttgat tccacgattg attgatgaat tgcccattat
     7801 tgccctgctt gcgactcaag cccaaggtgt aacagttatc aaggatgctg aggagctcaa
     7861 ggtcaaggaa acagaccgta ttcaggttgt ggcagacgcc ttaaatagta tgggagcaga
     7921 tattactcct acggcagatg ggatgattat caaagggaaa tcagctcttc acggtgctag
     7981 agtcaatacg tttggtgacc accgtatcgg catgatgaca gctatcgcag ccctcttggt
     8041 tgcagatgga gaggtggagc ttgaccgtgc agaagccatc aataccagct atcctagttt
     8101 ctttgatgat ttggagagct tgattcatgg ctaaggtgtt attagggttt atgggggctg
     8161 gaaaatcgac tattgcaaga ggcttggacc ctaattacct tgatatggat gctctgattg
     8221 agaagcgcct aggtatgtcc attgcgaatt ttttcgctga aaagggagaa gcagcctttc
     8281 gtcaggtaga atcagaagtc ctagctgatt tactacaaac agaccaagtc gtgtcaactg
     8341 gaggaggagt ggttatttct cagagaaatc gtgacttact caagactaat acagataaca
     8401 tctacctgaa agcagatttt gaaaccctct accaacgtat cgcagctgat aaggacaatc
     8461 agcgaccgct ttttctaaat aatagcaagg aagaactagt agctattttt caagaaagac
     8521 aggcttggta tgaggaagtg gctagtcggg ttttggatgt gaccaagcta agcccagagg
     8581 aaattataga ggaactaaga tgaaaattgc ttatctaggt cccaagggat cattttcaca
     8641 ccacgttgtg cagatagctt ttcctcatga ggaattgcag gcttttgcta acattacaga
     8701 tgtcatcaag gcttatgagc aaggattagt ggactattct gtggtgccag ttgaaaattc
     8761 tattgagggt agtgttcatg aaaccttgga ctatcttttt catcaggctc acatccaagc
     8821 agtagcagaa atcgttcagc ctattcatca gcagttgatg gtggttccag gccatactaa
     8881 gattgaaaag attttttcac atccacaggc cttggctcaa ggaaagaaat tcatcgatga
     8941 acaatatcca gaggctcaaa tcgaggtaac agctagtaca gcttatgcgg cccgttttat
     9001 ttccgaacat ccagaccagc cctttgcagc agttgcacct agaagttctg ctgaagaata
     9061 tggattggaa ctgattgccg aggatattca ggaaatggaa gccaatttca cacgtttctg
     9121 ggttctagga gctgaaaagc ctagtattcc cttgcaagca caaactgaaa agatgagttt
     9181 ggccttgaca ttacctgaca accttccagg tgcactttat aaggccctgt cgacctttgc
     9241 ttggcgagga attgacttga caaaaattga aagtcgtcca ctcaagacag cactgggtga
     9301 atactttttc attatcgatg tggattatac cgataaggac ttggtccact ttgcccaaaa
     9361 agaattagaa gcgattggaa tccagtataa aattctgggt gcctatccta tttatccaat
     9421 atcagaccat ggaaaggaga gaagatgagt aaagaaaatc ccttaagtca tcatgagcag
     9481 ttgcgttatg attatttgct aaaaaatatt cactatctca atgagagaga aaaaaatgag
     9541 tttgcctatt tgcaagaaaa gctaactctt gctaggggaa atagtagctc tagcttggaa
     9601 caagaaagag aagagcaggt tgacttacca agctatgcga accggagtcg ctcacaatcc
     9661 aaatcacaag cactctcttt ccctccaaaa aagaaaagac ggaagctccg tcttaaacga
     9721 atttttatgg tgattttttc acttttggtc tgtgtggctt tggccatggt attcatgttt
     9781 ttgcgtggtt accaagatgc tagcgcaaag aaaactgctg atgcccgggc agctcaagta
     9841 gaagtcttta atggtcagga cactagagat ggagttaata ttttaatcat gggtactgat
     9901 ggtcgaatcg gccagaacag tgttgagaca cgaactgact ctattatggt attaaatgtc
     9961 gggggctcag ataagaaaat gaagctagtc agtttcatgc gtgataattt ggtctatata
    10021 gatggttata gtcaagtgat taacggtaga aaacagacag ataacaagtt aaacgtagcc
    10081 tacgagttag gagaacaaga ggggcaaaaa ggggcagaaa tggttcgcca agtcttgaaa
    10141 gataattttg acttggacat taagtactac gccttggttg atttccaggc ctttgccaca
    10201 gcgattgaca cgcttttccc tgatggggtg acgattgatg ctcaattttc aacattgaat
    10261 gggcgtcccc taacagaagc tacagtcgga gatgatttat acgctaccga gactgagtct
    10321 ccaacccaaa ccattaaagt cggaaaacag cagatgaatg gctcgaccct gctcaattat
    10381 gctcgtttcc gtgatgatga tgaggcggat tacggccgta caaaaagaca gcaacaagtt
    10441 ttaacagcaa ttttagagca aattaaagat cccactaaac ttttcactgg ttctgaagct
    10501 cttggaaagg tttttgctat gacctcaacc aacgtaccct atactttcct cctaacaaac
    10561 ggtttatctg ttttggatgg agcaaaaaat ggtattgaaa aattgacgat tccagaactg
    10621 ggtgactggg tagatgccta tgatgtttat ggaggcttgg gcttgctggt tgatcaaaac
    10681 aaatatcaaa ccaagctcgc tcaaatgggc ttgagataag ataatactca atgaaaatca
    10741 aagtgcaaac taggaagcta gccgcaggct gctaaaaaca cagttttgag gttgtagata
    10801 agactgacga agtcagttac ctatacctac ggcaaggtga agctgacgtg gtttgaagag
    10861 attttcgaag agtataagac agaaaaatca aagtttgata gctatttgga tagcagtcag
    10921 gctttgattt tttacagtct gcaatagatt ttcacccctt gtttgtggta taatgaaacg
    10981 atacgataga aagaataatg aacaatatga ctgatttaaa agcaattcag gctcgtagtc
    11041 tggagatggc tgaatatttt gtggcctttt gtaaagaaca tgatttgctc tgttatctct
    11101 gtggcggagg cgctattggt gcccttcgaa acaagggatt tattccttgg gacgacgacc
    11161 tagacttttt tatgcctcgt aaagattatg agaaattagc agaattatgg cctcgttatg
    11221 cagatgaacg ttatttcttg tcaaagagtc acaaggattt tgttgatcgt aatcttttta
    11281 ttaccattcg tgacaaggaa accacctgta tcaagcctta tcagcaggat ttggatttgc
    11341 cacatggtct ggccttggat gttttgcctt tggattatta tccgaaaaat ccagctgagc
    11401 ggaaaaaaca ggttcgttgg gccttgattt attcactctt ttgtgcgcaa actattccag
    11461 aaaagcatgg tgctctcatg aaatggggaa gtcgcatttt actgggtttg actccaaaat
    11521 ctctctgtta tcgcatctgg aaaaaagctg agaaagaaat gactaagtat gatttggctg
    11581 attgtgatgg cattacagaa ttatgctcag gtcctggcta catgagaaac aagtacccaa
    11641 tcacatcttt tgaagacaat cttttcttgc catttgaagg aacagagatg cctattccaa
    11701 tcggctatga tgtctatctc agaactgctt ttggggatta tatgacgcct ccaccagcag
    11761 acaagcaggt accgcatcat gatgctgtca tcgctgatat ggataagtct tatacagaat
    11821 acaagggaga atatggtggc taagaaaaaa atcttatttt ttatgtggtc tttttctctt
    11881 ggaggtggtg cagagaagat tctatcaacc attgtttcaa atctggatcc agaaaagtat
    11941 gatattgata ttcttgaaat ggagcacttt gacaagggat atgaatctgt tccaaagcat
    12001 gtacgcattt taaaatccct tcaagattat cgccaaacca gatggttacg agcttttttg
    12061 tggagaatga gaatttattt tccaagactg gctcgtcgtt tgcttgtaaa agatgattat
    12121 gatgttgaag tttcttttac cattatgaat ccaccactgt tgttctctaa aagaagagaa
    12181 gtcaagaaga tatcttggat tcatggaagt attgaagaac ttcttaagga tagctctaaa
    12241 agagaatcac atagaagcca gttggatgct gcgaatacaa ttgtagggat ttcaaaaaag
    12301 accagcaatt ctatcaagga agtttatcca gattatactt ctaaattaca gacaatctac
    12361 aatggatatg attttcagac tattctagaa aaatctcaag agaagatcga tatcgagatt
    12421 gctcctcaaa gtatctgtac tatcggacgg attgaggaaa ataagggttc tgaccgtgta
    12481 gtggaagtga tacgattatt acaccaagag ggaaaaaact atcatctcta ttttatcggg
    12541 gctggtgata tggaagagga actgaaaaaa cgagtcaaag agtatgggat tgaggactat
    12601 gtacatttcc ttggttatca aaaaaatcct tatcagtatc tatctcagac gaaagttctt
    12661 ttgtctatgt ctaaacaaga aggttttcct ggagtgtatg tggaggcctt gagtctggga
    12721 ctccctttta tctctacgaa cgttggaggg gctgaggaat tatcccaaga aggacgattt
    12781 ggacaaatca ttgagagcaa tcaagaggca gctcaggcga ttactaatta catgacttct
    12841 gcctcaaact ttgatgtcga tgaggctagc caattcattc aacaatttac aattacaaaa
    12901 caaatcgaac aagtagaaaa actattagag gagtagcatg gaaactgcat taattagtgt
    12961 gattgtgcca gtctataatg tggcgcagta cctagaaaaa tcgatagctt ccattcagaa
    13021 gcagacctat caaaatctgg aaattattct tgttgatgat ggtgcaacag atgaaagtgg
    13081 tcgcttgtgt gattcaatcg ctgaacaaga tgacagggtg tcagtgcttc ataaaaagaa
    13141 cgaaggattg tcgcaagcac gaaatgatgg gatgaagcag gctcacgggg attatctgat
    13201 ttttattgac tcagatgatt atatccatcc agaaatgatt cagagcttat atgagcaatt
    13261 agttcaagaa gatgcggatg tttcaagctg tggtgtcatg aatgtctatg ctaatgatga
    13321 aagcccacag tcagccaatc aggatgacta ttttgtctgt gattctcaaa catttctaaa
    13381 ggaatacctc ataggtgaaa aaatacctgg gacgatttgc aataagctaa tcaagagaca
    13441 gattgcaact gccctatcct ttcctaaggg gttgatttac gaagatgcct attaccattt
    13501 tgatttaatc aagttggcca agaagtatgt ggttaatact aaaccctatt attactattt
    13561 ccatagaggg gatagtatta cgaccaaacc ctatgcagag aaggatttag cctatattga
    13621 tatctaccaa aagttttata atgaagttgt gaaaaactat cctgacttga aagaggtcgc
    13681 ttttttcaga ttggcctatg cccacttctt tattctggat aagatgttgc tagatgatca
    13741 gtataaacag tttgaagcct attctcagat tcatcgtttt ttaaaaggcc atgcctttgc
    13801 tatttctagg aatccaattt tccgtaaggg gagaagaatt agtgctttgg ccctattcat
    13861 aaatatttcc ttatatcgat tcttattact gaaaaatatt gaaaaatcta aaaaattaca
    13921 ttagaacggg ggtgaccaag tgattgatgg gaaacgatta ttatttagtt tgaccatagt
    13981 cagttatgcc ttgacgctag taagtggaat tgtgtatctg tttaataata ataatgttag
    14041 cttactttct actttattgt tcttgttggt tagtagctta attgcttgtt ggaatgatat
    14101 caagtattac ttaatccatt ttattttcta tttaaccatt tttgtatttc tggtatcaag
    14161 accgaccatt gattatttta gggatggtgc tttggatacc tatcatccaa tagcctatcg
    14221 ttttgccttt atagttgtca tggtttcgat tctgggcttg accacaggag gcattctggc
    14281 tcgttacttc atagctagga agaaaataaa agtagcaaat ataggaaatt ctctaaaaga
    14341 ggtttatatc aagcggttac gctttgtatc actaggagtt tttcttctaa cctatccttt
    14401 ctatttcatt cggttatttg aacggctctt gtatcgtttg cagacttcct actatgccta
    14461 ctatgcaaat tttgaaagta aactgcctta ttttacctac attttgtcta cctttacggt
    14521 ctatgcaatg tgtatgtatc tggcaaccaa gccaaagaaa ttgcaggcca cagcagtgct
    14581 tgtctccttt attgcagcta atactattca tttggcaatt gggacacgaa atccctttat
    14641 tttaagtatt ttatttgctt ttgtttatta ctttatgcgg gagcaaactg aaaaaggaaa
    14701 atggattggg tttaaagaaa agttagcgat ttttgtaggt tctcctattc tcatgttagc
    14761 gatgggagta ctcaattatg tacgggataa tgtccaagtt tctcatacag gtttctggga
    14821 tatcttactt gactttatct ataaacaagg gactagtttt ggtgttctgg ctcgaggttt
    14881 tctatttaac agtagcctcc cttaccgaga tttccgtaat tttacttttg gtcctgttct
    14941 tgattatttt gcaaggggga gtttgggagc cattttcgga ggaaaagcct ttgaacatac
    15001 aaccaatagt gtggaactag ctattgatag taatagttat gcccacaatc tatcctatct
    15061 tgtcttgaac aaggaatact tgaaagggca tggtatcgga agtagttata tcatggagtt
    15121 gtataccgac tatggtatga ttggagtctt tctgcttagt ttcttactcg gcgtattatt
    15181 tatagccatg ctgcaagtag cctatcgctc aaggacaatc ctatttgctt tatccctact
    15241 catcttgaat aatctattct ttatgccaag aagcagcttt tcagaaagtt tcttcaattt
    15301 atttacaatg caattctggg gaattgttct tgtgattata tttgtagcaa aaatgcttac
    15361 aaaggaaaac tagtatctac taaacaaagg agaaaaaatc atgtttgaac attattcagt
    15421 agctgatttg tttgcaaatc tttataaaaa gcgaaaagca aatattttag ctctcatcgc
    15481 tttatttgct ctcattgctg taccatttac aattaaagca gttaggaata aaaacactgt
    15541 caaagataca acaagttatt caacttatct tatctataaa atcactcctc caaaagagtc
    15601 ggccaaaacg attttgaatc atcaaattgg tggttatagt gatttttatg ggaaattgat
    15661 tgatggtaat ttgaatggag cttatctttt caatgatgta gaacccagtg agttgaaaaa
    15721 aattgccagt gaattagaca cgacagaaac aaccttgaaa aattcaacga atgactattg
    15781 gtggaaaaaa ttgaccgtct actatatgat tgacgatgca ggggttggtg tgaaaatttt
    15841 gacatcaagt aaagatgcca atgacttgtt agagaaaaaa attgatgggt tgattgagaa
    15901 atttaaacat gcctatgcaa atgtgaaaat tgaaaaactg gaaaccatca actctaaaga
    15961 attgaacgca aatggtgaaa cagcgcttgg cttaaatgtg aaaaatctga ttcttcgttt
    16021 agttgttatt ggagtggttt gtgtgatttt ggttgtgatg ggaaatgtgt tagtttatct
    16081 ctttaatcca acaatcaata gagtaggtga tttttctcag tatcaaattg attttgtaac
    16141 agagattaca acaattgcta acctagcaga tgttttgtca tacaaaaata ctggacagga
    16201 attgaccatc gttagctcaa ataaagctat cctagataaa ttgaaacaga gtcaagaagc
    16261 tttaaaagga atgcattttg tagatttaca ggatgtatca tctcttttgg aaagagatac
    16321 agtccttctt gttgaagagt acggagtgac tcgttataag aaatttgagc aaagtcttca
    16381 aattctcaga aacttaaatc gttctatcct tggtgtagca acctttaaat tataagtatt
    16441 ctgatgtata aggtcttcaa aatcggctaa gagctgattt tgaaggcttt ttctatctgt
    16501 ctgcagaaat tttcttttgg agtctcctat agaaagaccc tagggaaaat ctatccgtat
    16561 agaggagcct tcttttgata aaatcgtaaa aatctagtat aatagataga actgaaagta
    16621 tgaggttact agcaatgaaa atgaaacaaa ttagtgatac aactttaaaa atcacgatgt
    16681 ctttagagga tttgatggat cgtggaatgg agattgctga ctttctcgtt cctcaagaaa
    16741 aaacagaaga gttcttttat gctatcttgg atgaactaga gatgcctgat agctttctgg
    16801 atacaggcat gttgagcttc cgtgtgactc caaaacctga taaggtagat gtctttgtga
    16861 ccaagtcaaa gattgaccaa aatctagatt ttgaagactt atcggatttg ccagatatgg
    16921 aagaattggc tcaaatgtct ccagatgaat ttatcaaaac cctggaaaaa agcatcgcag
    16981 acaaaaccaa ggatgatatc gaagccattc aatctcttga gcaagttgaa gccaaggaag
    17041 aagagcaaga gcaggctgaa caagaagctg agagtaagaa agaaccttac atctactaca
    17101 tcctttcttt tgctaagttg gctgacttgg tagtttttgc caagacagtg acttttgaaa
    17161 tggaaacttc tgaactctac aaaatgaacg agcgctatta tttgaccatt ttagtggata
    17221 ttgaaaatca tccaagccca tatccagctt ggctgttggc ccgtatgcgc gagtttgcag
    17281 acgatagtga tatcagtcgc tcagtcttac aagagtatgg tcaagtcttg atgagtcacg
    17341 atgcagtgct caatctgcaa aaaatcggct aatcctttct ggaaagctat tttcagattt
    17401 taagaagagc gaatcgtctg attcgttttt tctttttata ctgaaatagt gatttactat
    17461 aataggaatt ttcacaaaat tctgttataa tggctatatt agaaaatttc gaggagataa
    17521 agatgacagt taaaattgct ttactaggat ttggtaccgt tgcaagtggt gtgcctttcc
    17581 tcctaaagga aaatggagga aaaatcaatc aatcagcaca ttcagatatc aaagttgcta
    17641 aggtattggt caaggatgaa gatgaaaaaa atcgcttgct tgcagcaggg aatgacttta
    17701 actttgtaac caatgtggat gatattttat cagaccagga tattactatc gtagtggaat
    17761 tgatggggcg tattgagcct gctaaaacct ttatcactcg tgccttggaa gctggaaaac
    17821 acgttgttac tgctaacaag gaccttttag ctgtccatgg cgcagaattg ctagaaatcg
    17881 ctcaagctaa caaggtagca ctttactacg aagcagcagt tgctggtggg attccaattc
    17941 ttcgtacttt agcaaattcc ttggcttctg ataaaattac gcgcgtgctt ggagtagtca
    18001 acggaacttc caacttcatg gtgaccaaga tggtggaaga aggctggtct tacgatgatg
    18061 ctcttgcgga agcacaacgt ctaggatttg cagaaagcga tccgacgaat gacgtagatg
    18121 ggattgatgc agcctacaag atggttattt tgagccaatt tgcctttggc atgaagattg
    18181 cctttgatga tgtagcccac aagggaatcc gcaatatcac accagaagac gtagctgtag
    18241 ctcaagagtt tggttacgta gtgaaattgg ttggttctat tgaggaaact tcttcaggta
    18301 ttgctgcaga agtgactcca accttcctac ctaaagcgca cccacttgct agtgtgaatg
    18361 gcgtaatgaa cgctgtcttt gtagaatcta tcggtattgg tgagtctatg tactacggac
    18421 caggtgcggg tcaaaaacca actgcaacaa gtgttgtagc tgatattgtc cgtatcgttc
    18481 gtcgtttgaa tgatggtacc attggcaaag acttcaacga atatagccgt gacttggtct
    18541 tggcaaatcc tgaagatgtc aaagcaaact actatttctc aatcttggct ccagactcaa
    18601 aaggtcaggt cttgaagttg gctgaaatct tcaatgctca agatatttcc tttaagcaaa
    18661 tccttcaaga tggcaaagag ggtgacaagg cgcgtgtcgt tatcatcaca cacaagatta
    18721 ataaagccca gcttgaaaat gtctcagctg aattgaagaa ggtttcagaa ttcgacctct
    18781 tgaatacctt caaggtgcta ggagaataag atgaagatta ttgtacctgc aaccagtgcc
    18841 aatatcgggc caggttttga ctcggtcggt gtagctgtaa ccaagtatct tcaaattgag
    18901 gtctgcgaag aacgagatga gtggctgatt gaacaccaga ttggcaaatg gattccacat
    18961 gatgagcgta atctcttgct caaaatcgct ttgcaaattg taccagactt gcaaccaaga
    19021 cgcttgaaaa tgaccagtga tgtccctttg gcgcgcggtt tgggttcttc cagctcggtt
    19081 atcgttgctg ggattgaact agccaaccaa ctgggtcaac tcaacttatc agaccatgaa
    19141 aaattgcagt tagcgaccaa gattgaaggg catcctgaca atgtggctcc agccatttat
    19201 ggtaatctcg ttattgcaag ttctgttgaa gggcaagtct ctgctatcgt agcagacttt
    19261 ccagagtgtg attttctagc ttacattcca aactatgaat tacgtactcg cgacagccgt
    19321 agtgtcttgc ctaaaaaatt gtcttataag gaagctgttg ctgcaagttc tatcgccaat
    19381 gtagcggttg ctgccttgtt ggcaggagac atggtgaccg ctgggcaagc aatcgaggga
    19441 gacctcttcc atgagcgtta tcgtcaggac ttggtaagag aatttgcgat gattaagcaa
    19501 gtgaccaaag aaaatggggc ctatgcaacc tacctttctg gtgctgggcc gacagttatg
    19561 gttctggctt ctcatgacaa gatgccaaca attaaggcag aattggaaaa gcaacctttc
    19621 aaaggaaaac tgcatgactt gagagttgat acccaaggtg tccgtgtaga agcaaaataa
    19681 agaatagaag ataggatggg gaaactctta accagagggt ttcatatcct ttttgtgaaa
    19741 agaagtttat actcaatgaa aatcaaagag caaactagga agctagccgc aggctgctca
    19801 aaacagtgtt ttgaggttgc agatagaact gacgaagtca gctcaagaca ctgttttgag
    19861 gttgcagata gaactgacga agtcagctca agacactgtt ttgaggttgc agatagaact
    19921 gacgaagtca gtaacatata tacggtaagg tgacgctaac gtggtttgaa gagattttcg
    19981 aagagtatta gttaaaaacg tgataaagga gaaataaaga tggcagaaat ttatctagca
    20041 ggtggttgtt tttggggcct agaggaatat ttttcacgca tttctggagt gctagaaacc
    20101 agtgttggct acgctaatgg tcaagtcgaa acgaccaatt accagttgat caaggaaaca
    20161 gaccatgcag aaacggtcca agtgatttac gatgagaagg aagtgtcact cagagagatt
    20221 ttactttatt atttccgagt tatcgatcct ctatctatca atcaacaagg gaatgaccgt
    20281 ggccgccaat atcgaactgg gatttattat caggatgaag cagatttgcc agctatctac
    20341 acagtggtgc aggagcagga acgcatgctg ggtcgaaaga ttgcagtaga agtggagcaa
    20401 ttacgccact acattctggc tgaagactac caccaagact atctcaggaa gaatccttca
    20461 ggttactgtc atatcgatgt gaccgatgct gataagccat tgattgatgc agcaaactat
    20521 gaaaagccta gtcaagaggt gttgaaggcc agtctatctg aagagtctta tcgtgtcaca
    20581 caagaagctg ctacagaggc tccatttacc aatgcctatg accaaacctt tgaagagggg
    20641 atttatgtag atattacgac aggtgagcca ctcttttttg ccaaggataa gtttgcttca
    20701 ggttgtggtt ggccaagttt tagccgtccg atttccaaag agttgattca ttattacaag
    20761 gatctgagcc atggaatgga gcgaattgaa gttcgttctc gttcaggcag tgctcacttg
    20821 ggtcatgttt tcacagatgg accgcgggag ttaggcggcc tccgttactg tatcaattct
    20881 gcttctttac gctttgtggc caaggatgag atggaaaaag caggatatgg ctatctattg
    20941 ccttacttaa acaaataaaa cagagagtgg ggcttcccac tttcttcatt tctagaatat
    21001 gaatagaagg gatttatgaa acacctatta tcttacttca aaccctacat caaggaatca
    21061 attttagccc ccttgttcaa gctgttagaa gctgtttttg agctcttggt tcccatggtg
    21121 attgctggga ttgttgacca atctttacct cagggagatc aaggtcatct ctggatgcag
    21181 attggcctgc tccttatctt tgcagtaatt ggcgttttag tggccttgat agctcaattt
    21241 tactcagcaa aggcagcagt aggttttgct aaggaattga caaacgatct ttatcgtcat
    21301 attctttcct tgccaaagga cagcagagac cgtctgacaa cttctagttt ggttactcgc
    21361 ttgacttcgg atacctacca gattcagact ggtatcaatc aattcctgcg tctcttttta
    21421 cgagcgccca ttatcgtttt tggtgccatt tttatggctt atcgaatctc agctgagttg
    21481 actttctggt tcttagtcat ggttgccatt ttgaccattg tcattgtagg gttatctcga
    21541 ttggtcaatc ctctctacag tagtctcaga aagaaaacgg accaactggt tcaggaaacg
    21601 cgccagcaat tgcaagggat gcgggttatt cgtgcttttg gtcaagaaaa acgagagtta
    21661 cagatttttc aaacccttaa ccaagtttat gctagattac aagaaaagac aggtttctgg
    21721 tctagtttat taacacctct gacctatctg attgtcaatg gaactcttct cgttattatc
    21781 tggcaaggct atatttcaat tcaaggagga gtgctcagtc aaggtgctct cattgctctt
    21841 atcaattacc tcttacagat tttggtggaa ttggtcaagc tagccatgtt gataaattct
    21901 ctcaaccagt cctatatctc agccaagcga atcgaggagg tctttgcgga atctcccgaa
    21961 aacatccatt cagaattaga acaaaagcaa gttaccagtg gtcgggtttt acaagtccaa
    22021 gaattgactt ttacctatcc tgatgcggcc cagccttctc tgagatacat ttcctttgat
    22081 atgactcaag gacaaattct aggtatcatc gggggaacgg gttctggtaa gtcaagcttg
    22141 gtgcaactct tacttggact ttatccaata gataagggga gcattgacct ttatcgagat
    22201 ggatctagtc ctcgtaattt agagcagtgg cggtcttgga ttgcttatgt tccccaaaag
    22261 gtcgaacttt ttaagggaac tattcgttcc aacttgactt taggtttaaa tcaagaagtg
    22321 actgatcaag aactctggca agccttggaa attgctcaag ctaaggattt tgtcagtgat
    22381 aaggaaggac tcttggatgc tctagttgaa gcaggagggc gaaatttctc aggtggacaa
    22441 aaacaaaggc tgtctatcgc acgagcagtc ttgcgccagg ctccgtttct catcctagat
    22501 gatgcgacct cagcactgga caccattacc gagtccaagc tcttgaaagc tattagagaa
    22561 aattttccaa acatgagctt aattttgatc tctcaacgaa cctcgacttt acagatggct
    22621 gaccagattc tcctcttgga aaaaggtgag ttgctagctg ttggcaagca cgatgacttg
    22681 atgaaatcca gtcaagtcta tcgtgaaatc aatgcatccc aacatggaaa ggaggactag
    22741 aatgaaacga caaactgtaa accagacgct caaacgttta gccgtagatt tagcaaacca
    22801 tcccttcctc cttttcctag cctttctagg aactattgcc caagttggct tatcaattta
    22861 tctacctatt ctgattgggc aggtcattga ccaagtccta gtggctggtt catcaccagt
    22921 tttttggcag atttttctcc agatgctctt ggtggtaata ggaaatactc tagtacaatg
    22981 ggccaatcct ctcctctata atcgtctaat cttctcttat accagagatt tacgagagcg
    23041 aatcatccat aaactccatc ggttaccgat tgcttttgcg gatcggcaag gcaatggaga
    23101 gatggtcagt cgtgtaacca cggacatcga acagttggca gctggcttga ccatgatttt
    23161 taaccaattt ttcattggtg ttttgatgat tttggttagt attctagcca tgctccaaat
    23221 tcatctcctc atgactctct tagtcttgct gttgacgcca ctgtccatgg tgatttcacg
    23281 ctttattgcc aagagatcct atcatctctt ccagaagcaa acagagacga ggggaattca
    23341 gactcagttg attgaagaat cgcttagcca gcagactata atccagtcct tcaatgctca
    23401 aacagaattt atccaaagat tgcgtgaggc tcatgacaac tactcaggct attctcagtc
    23461 agccatcttt tattcttcaa cggtcaatcc ttcgactcgc tttgtaaatg cactcattta
    23521 tgccctttta gctggagtag gagcttatcg tatcatgatg ggttcagcct tgaccgtcgg
    23581 tcgtttagtg acttttttga actatgttca gcaatacacc aagcccttta acgatatttc
    23641 ttcagtgcta gctgagttgc aaagtgctct ggcttgcgta gagcgtatct atggagtctt
    23701 agatagccct gaagtggctg aaacaggtaa ggaagtcttg accagtgacc aagttaaggg
    23761 agctatttcc tttaaacatg tctcttttgg ctaccatcct gaaaaaattt tgattaagga
    23821 cttgtctatc gatattccag ctggtagtaa gatagccatc gttggtccga caggtgctgg
    23881 aaaatcaact cttatcaatc tccttatgcg tttttatccc attagctcgg gagatatctt
    23941 gctggatggg caatccattt atgattatac acgagcatca ttgagacagc agtttggtat
    24001 ggtgcttcaa gaaacctggc tcacacaagg gaccattcat gataatattg cctttggcaa
    24061 tcctgaagcc agtcgagagc aagtaattgc tgctgccaaa gcagctaatg cagacttttt
    24121 cattcaacag ttgccacagg gatacgatac caagttggaa aatgctggag aatctctctc
    24181 tgtcggccaa gctcagctct tgaccatagc ccgagtcttt ctggctattc caaagattct
    24241 tatcttagac gaggcaactt cttccattga tacacggaca gaagtgctgg tacaggatac
    24301 ctttgcaaaa ctcatgaagg gccgcacaag cttcatcatt gctcaccgtt tgtcaaccat
    24361 tcaggatgcg gatttaattc ttgtcttagt agatggtgat attgttgaat atggtaacca
    24421 tcaagaactc atggatagaa agggtaagta ttaccaaatg caaaaagctg cggcttttag
    24481 ttctgaataa gccattctct tttgaaagtt tatggacgaa aaaagttgcc ttcgagtgac
    24541 ttttttgtta caatagctag aaaaattgtt cactgtaata ctcaatgaaa atcaaagagc
    24601 aaactaggaa gctagccgta ggttgctcaa agcacagctt tgaggttgta gataagactg
    24661 acgaagtcag ttcaaaacac tgttttgagg ttgcagatag aactgacgaa gtcagctcaa
    24721 aacactgttt tgaggttgca gatagaactg acgaagtcag taaccacacc tacggcaaag
    24781 tgaatctgaa gtggtttgaa gagagtacaa cttgtctttt aaaaaaggag tctataatga
    24841 aagtctttca gcatgtaaat atcgtgactt gtgatcaaaa tttccatgtt tatcttgatg
    24901 gaatcttagc agtcaaggat tctcaaatcg tctatgtcgg tcaagataag ccagcgtttt
    24961 tagaacaagc tgagcagatt atagactatc agggagcttg gattatgcct ggtttggtca
    25021 attgtcacac ccattctgca atgacaggtc tgagagggat ccgagatgac agcaatctcc
    25081 atgaatggct caatgactat atctggccag cagaatctga gtttactccc gacatgacta
    25141 ccaatgcggt caaagaagcc ctgacagaga tgctccagtc aggaacaaca acctttaacg
    25201 atatgtataa tcccaatggt gtggatatcc agcaaattta tcaggtggtg aaaacttcca
    25261 agatgcgttg ttatttttct ccgactctct tttcttcaga gacagaaaca actgctgaga
    25321 ctataagcag aactcgatcc atcatagacg aaatcttaaa atataaaaat ccaaatttca
    25381 aggttatggt agcacctcat tctccgtata gctgcagtag agacttgctg gaagcgagtt
    25441 tggaaatggc aaaagagcta aatattccgc tccatgtcca tgtggcggag accaaggagg
    25501 agtcaggaat tatcctcaaa cgatacggca aacgccccct tgcttttctt gaagaactgg
    25561 gttatttaga tcatccgtcc gtatttgctc acggggtcga attaaacgag agagaaattg
    25621 aacgcttggc atcttctcaa gtggctatcg cccacaatcc tatcagtaac ctcaaactgg
    25681 catcaggaat tgctccaatt atccagctcc aaaaagcggg agtagcagtc ggaattgcga
    25741 ctgactcggt tgcttccaat aacaatctag atatgtttga ggaaggaagg actgcagctc
    25801 ttcttcagaa gatgaaaagt ggggatgcca gccagtttcc aatcgaaaca gctctcaagg
    25861 tactgacaat cgaaggggct aaggtccttg gaatggaaaa tcagatagga agtctggaag
    25921 tcggcaagca agcagatttt ctggtcattc aaccacaagg gaaaattcat ctccaacctc
    25981 aggaaaatat gctgtctcac ctggtttatg cagttaaatc tagtgatgta gatgatgttt
    26041 atatcgccgg agaacaggtt gttaagcaag gtcaagtcct gacagtagaa ctttaaaaga
    26101 aaaatcacga aaaattttaa aaaaagtttg caaaaatctt gcattctttt tttgactatg
    26161 ctatacttat atacggttta aaaaaactgc ctaagacagt aggggagatc gactcataaa
    26221 tatcctaccg aggacaaaac gtatcatgta aaaagaagcg tattgtactt tcgtgtctag
    26281 gtttgggcgc gtttttcttt ttgaaaaatt ccccaagcaa aataattacg gaggtgaaca
    26341 cactaatgag tgaagcaatt attgctaaaa aagcggaact agttgacgta gtagctgaaa
    26401 aaatgaaagc tgctgcatct atcgtcgttg tagacgctcg tggtttgaca gttgagcaag
    26461 atacagttct tcgtcgtgag cttcgtggaa gcgaagttga gtataaagtt attaaaaact
    26521 caatcttgcg tcgtgcagct gaaaaagctg gtcttgaaga tcttgcatct gtatttgttg
    26581 gaccatctgc agtagcattt tctaatgaag atgttatcgc accagcgaaa atcttgaacg
    26641 acttttctaa aaacgctgaa gcacttgaaa ttaaaggtgg tgcaatcgaa ggcgctgtcg
    26701 catctaaaga agagattctt gcacttgcaa ctcttccaaa ccgcgaagga cttctttcta
    26761 tgctcctttc tgtacttcaa gcgccagtgc gcaacgttgc tcttgcagtc aaagcggttg
    26821 cagaaagcaa agaagacgcg gcttaatctt aagctacata gcgtagccta gctacgaaaa
    26881 aaactattat aaaatttaaa acttatttgg aggaaataac aatggcattg aacattgaaa
    26941 acattattgc tgaaattaaa gaagcttcaa tccttgaatt gaacgacctt gtaaaagcta
    27001 tcgaagaaga atttggtgta actgcagctg ctcctgtagc tgttgctgca gctgatgcag
    27061 ctgatgctgg tgctgctaaa gattcattcg acgttgaatt gacatctgca ggcgacaaaa
    27121 aagttggcgt tatcaaagtt gtacgtgaaa tcactggtct tggtcttaaa gaagctaaag
    27181 aacttgttga cggtgcacca gcacttgtta aagaaggcgt tgcaactgca gaagctgaag
    27241 aaatcaaagc taaattggaa gaagctggag cttcagttac tcttaaataa tagagtatcg
    27301 caatttgatt gcggaatccc tatttaccag tctttgagag cgataagcga ttgatagaaa
    27361 ctgataaatt gatttggttt cctgccaaaa attctttggc aggatttttg ttttaataaa
    27421 ctttcggggg tagacactag attgtctacc cctttttttg tgtttcggag gtaattatgc
    27481 aaaaggaagg acagtcatca cgtcaattcg tgaagagtca tctcatgact atcatgggag
    27541 ttgatattgt aaaagcaact cagttagttg atgaaatgga acaagtaggt ttaatccgat
    27601 ttgatgaatt tggaaatgtt ggaattttag tcttggaggg acaataatga agcgtattac
    27661 cgctaatcaa tatcagacat cagagcgtta ttataagctc cccaaacttt tatttgagag
    27721 tgaacggtat aagaatatga aactggaggt taaggtggtt tactcagttt taaaagatcg
    27781 gttggagttg tctttgagca aaggttggat tgatgaggat ggggctattt atttgattta
    27841 ttccaattca aatttgatgg cacttttagg ctgttcaaag tcaaaattac tctccatgtg
    27901 agtttgaagt gacattttta gatgattacc ataaaaaaca taactaccca ctattttacg
    27961 aatcctatct tcaaaacgtt atggaattcc ttgaaagtca agacataaag aatggggttg
    28021 atgcctttgt agatgatcat caaaatctcg tttttgtttt atatggacaa ggctatcgag
    28081 ccgagggaaa agagggaata cttacaaccc aagtaactgt aaaagcttat gatgaagaca
    28141 agaaaccgat taacttcgca aatttattag attccttaat ttattagatt ccttaatcgt
    28201 gtcagaatat caaatggaac cgaatctttg ggaggtctcc catgattgat ctctatctaa
    28261 gtaaaaatag acaaagaaat caacttcttt tagatttctt ccaaaactat ggcatagagg
    28321 tatcttgtca ttcagtttct gaaatgacaa aggacaaatt aattgagatg atgagctatt
    28381 cttcagattg ttttgagttt ttatctccca atttattacg atttaagaac cgtgacaatc
    28441 taagattaac ggatttcgtt gagataattt taaaaaatcc tgaactaacc atcagacttc
    28501 ctcttgcggt ttcaaataag cgagtttatc caagtctgaa tttggaagag gctagagctc
    28561 tattgccaag agatacgaaa caattgattt acatggcgca aacacattat ttatctaact
    28621 aaaggagaag caatatggct gaacgatttt gggagaactt gtccattatt ttggctgaaa
    28681 gaaatatcag ctggattgag ttaaccagaa aaatgtttgc gggagagttt cactatccaa
    28741 gtgaattgaa tcgtttgtat caaaagattc gccactagaa gatggaacaa tgaatgcctc
    28801 aaagtccatg ggtagaaagg attgtgcagg tattagattt agattatgaa gatttatttc
    28861 ggaggtaact atgaaaagaa taattccagt ttatatattc caacaagtaa atgtcctatt
    28921 ggtatctcta tacttattga aatttctttg tatcggtgag ttaactatac tacagattct
    28981 ctatggttcg tcactcattt cttttttatg gatgtatggc caacgcaaac aagcgcataa
    29041 ggtcaatatg aaatctagga tgaaatggct tggtgttgaa tttgttagcc tactgattat
    29101 aagtctatgt tttagtctga ttcatgctca aggaagcacg aatcaagcaa acttaattgg
    29161 ccttcaacat caaattcctt ggttttcatt tttattgttc ttaatcaatg cgagtatggt
    29221 tgaagaattt ctgtatcgag aaattttatg gaatttggtc agaaaattag atatcagagt
    29281 tgctttgaca agtgttttat ttgccttagc acatcataca ggaaccattc tagcttggtg
    29341 tttgtatgtt tcacttggga tgtttttagg tatggtgcgc tataaatctg acttatgggg
    29401 cagtatggga ctacatttgg tgtggaacct actagtttat agtttgctgc ttttttaaaa
    29461 taggtgacct gatttttagg taacttctaa aactaacttc cagttttggg agagagatgg
    29521 aagttacttt gaaaagttac ggaaacatta ttttctatgt gacaacattg tataattgct
    29581 tgcattttac tggtggagag agtataatga gggtggataa agtacttaat cccaaaaatt
    29641 taaataggag gagctagcta tggcagaaga agtattaaat cttcaattag tatctgttca
    29701 agttgacgaa acagatgaag ttgatggaat gcgatttagt acattcagta caaatcgttg
    29761 cggt
//
LOCUS       DAVLVF010000020        26383 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVLVF010000020 DAVLVF010000000
VERSION     DAVLVF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26383
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.020"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            144..1007
                     /locus_tag="VYN66_000848"
     CDS             144..1007
                     /locus_tag="VYN66_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1494905.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSILSHLKK
                     EVEIYYQAKER"
     gene            complement(1296..2687)
                     /gene="argH"
                     /locus_tag="VYN66_000849"
     CDS             complement(1296..2687)
                     /gene="argH"
                     /locus_tag="VYN66_000849"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV1494906.1"
                     /translation="MAKNTKLWGGRFEGTVEDWVERFGASISFDQKLAKFDVIGSLAH
                     VQMLGQTGILSLEESEKIQVGLKELLEELEAGQLDFDIANEDIHMNMEVLLTEKIGPL
                     AGKLHTARSRNDQVATDMHLYLKEQLGYVLDKLAHLKGVLLDLAENHVATIMPGYTHL
                     QHAQPISFAYHLMAYYNMFQRDSERFEFNQKHTDLCPLGAAALAGTTFPIDRQLSSDL
                     LEFKQPYTNSLDAVSDRDFILEFLSNASILMMHMSRFCEEMINWCSFEYQFITLSDTF
                     TTGSSIMPQKKNPDMAELIRGKTGRVYGHLFGLLTVMKSLPLAYNKDLQEDKEGMFDT
                     VETILNSLDVLAGMLSSLQVNKEKMQESTEKDFSNATELADYLAGKGLPFREAHEVVG
                     RLVLDSIKSAKNLQDWTLEELQTYHSLITEDIYVYLQPKTAVQRRNSLGGTGFDQVEY
                     QIAVAKKANEAKK"
     gene            complement(2716..3913)
                     /locus_tag="VYN66_000850"
                     /pseudo
     CDS             complement(2716..3913)
                     /locus_tag="VYN66_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031904.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
     gene            complement(3930..4736)
                     /locus_tag="VYN66_000851"
     CDS             complement(3930..4736)
                     /locus_tag="VYN66_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1494907.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLVTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYSVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(4943..5584)
                     /locus_tag="VYN66_000852"
     CDS             complement(4943..5584)
                     /locus_tag="VYN66_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494908.1"
                     /translation="MIELENITKTIGGKVILDRLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLSKKDKLKKVEEILERVGLSATLHQKVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            complement(5581..7689)
                     /locus_tag="VYN66_000853"
     CDS             complement(5581..7689)
                     /locus_tag="VYN66_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1494909.1"
                     /translation="MKKISHLCMLLLLLCTTFFVLNVNVTREIVRIQEMGKTTYSFDL
                     YLKDVTKPTETILQFFEEVASQDKVSIIKVDNGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLLFNYLMKIGTTILLTVL
                     LVGVGKSLEQENKELDYQKQWISQGNYLTLETFQLNDNLWQEQLAGSGKALDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLFSMMISYFLLVITFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSLPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     ALASRMEKRSLVELLKGGIL"
     gene            complement(7744..8037)
                     /locus_tag="VYN66_000854"
     CDS             complement(7744..8037)
                     /locus_tag="VYN66_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1494910.1"
                     /translation="MIGEINHEKISVASLMVCSILASTSVLAVWVDGGQWNYGVGWSG
                     NFGYSDYLHSTRSHTATVKDGNKFSKDRAEAEAWARASIFKFPPTGMEYFYGF"
     gene            8443..8763
                     /locus_tag="VYN66_000855"
     CDS             8443..8763
                     /locus_tag="VYN66_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1494911.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(8815..9402)
                     /locus_tag="VYN66_000856"
     CDS             complement(8815..9402)
                     /locus_tag="VYN66_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1494912.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            9644..10315
                     /gene="sdaAB"
                     /locus_tag="VYN66_000857"
     CDS             9644..10315
                     /gene="sdaAB"
                     /locus_tag="VYN66_000857"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV1494913.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            10324..11196
                     /gene="sdaAA"
                     /locus_tag="VYN66_000858"
     CDS             10324..11196
                     /gene="sdaAA"
                     /locus_tag="VYN66_000858"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV1494914.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            11218..11850
                     /locus_tag="VYN66_000859"
     CDS             11218..11850
                     /locus_tag="VYN66_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1494915.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            11876..11974
                     /locus_tag="VYN66_000860"
                     /pseudo
     CDS             11876..11974
                     /locus_tag="VYN66_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(12156..14006)
                     /locus_tag="VYN66_000861"
     CDS             complement(12156..14006)
                     /locus_tag="VYN66_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1494916.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(14048..15205)
                     /locus_tag="VYN66_000862"
     CDS             complement(14048..15205)
                     /locus_tag="VYN66_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1494917.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENVEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            15263..16429
                     /locus_tag="VYN66_000863"
     CDS             15263..16429
                     /locus_tag="VYN66_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV1494918.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLTIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIYILMTVALFTVDRADKIL"
     gene            16568..16894
                     /locus_tag="VYN66_000864"
     CDS             16568..16894
                     /locus_tag="VYN66_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1494919.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            16881..17474
                     /locus_tag="VYN66_000865"
     CDS             16881..17474
                     /locus_tag="VYN66_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV1494920.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVTRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            17467..18378
                     /locus_tag="VYN66_000866"
     CDS             17467..18378
                     /locus_tag="VYN66_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV1494921.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            18441..19505
                     /locus_tag="VYN66_000867"
     CDS             18441..19505
                     /locus_tag="VYN66_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494922.1"
                     /translation="MKQEWFESNNFVKTTSKNKPEEQAQEVADKAEETIADFDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(19577..20437)
                     /locus_tag="VYN66_000868"
     CDS             complement(19577..20437)
                     /locus_tag="VYN66_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV1494923.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDDSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILV
                     QDFKTLAQEK"
     gene            complement(20559..21545)
                     /locus_tag="VYN66_000869"
     CDS             complement(20559..21545)
                     /locus_tag="VYN66_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV1494924.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(21817..23292)
                     /locus_tag="VYN66_000870"
     CDS             complement(21817..23292)
                     /locus_tag="VYN66_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV1494925.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(23550..24473)
                     /locus_tag="VYN66_000871"
     CDS             complement(23550..24473)
                     /locus_tag="VYN66_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1494926.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(24487..25416)
                     /locus_tag="VYN66_000872"
     CDS             complement(24487..25416)
                     /locus_tag="VYN66_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1494927.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(25735..25956)
                     /locus_tag="VYN66_000873"
     CDS             complement(25735..25956)
                     /locus_tag="VYN66_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494928.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            complement(25910..26170)
                     /locus_tag="VYN66_000874"
     CDS             complement(25910..26170)
                     /locus_tag="VYN66_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494929.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALTDDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT         7945 a         5736 c         4769 g         7933 t
ORIGIN      
        1 cttattaagc tcattttaaa attacgttaa cttttttcac aaatttattt cgactttctt
       61 ttttactttt tacattgcta tgatacaata tttttaacaa aattttattg tttgttaact
      121 tttttcacga aaaggagaaa atcatgcgtt atgatttcgg aaaggtctat aaagaaatac
      181 gtgagtcaaa ggggctgacc caagaagagg tctgtggggg tgttctctca agaaccagct
      241 tatcaaaaat cgaaagtggc aagacaaccc caaaatatga aaatatggaa tttcttctcc
      301 gtcaaatcaa tatgagtttt gaagagtttg aatatatctg tcaactttat caaccaagtc
      361 aacgcacaga gattatgcaa acctatctca atatgcgttc cattatcggg accagcgatc
      421 ttgtcaattt atttcaaaaa tgccaagact acctcaagac ccaccacgat ctccctatag
      481 aagaaatcag agatatgctt gaagttgtta tttatctccg tcaacacggg gcaggggaac
      541 tatcaaaaca cgcagaacag gttgtcaaaa aactttggaa aaaaattgaa aaacaagata
      601 catggtatga gagtgatcta aaaatcctca ataccattct tttcagtttt cccattgaat
      661 acctccatct catcactgga aaaatattgc aacgcctaga agtctataaa aactatcaac
      721 atttatatga cttgcgaatg acaattctat taaacctttc cacactctat ctatacaatc
      781 aagataaaaa catgtgtaag caaatctgct acactttact ggaggacgcc aagaataaga
      841 aaagctacga taggctggct atctgctatg tccgtattgg gatttgtagg gatgatgcga
      901 aacttatcca aaaagggttc tcccttctgg agctaaccga ggaaacttct atactgtctc
      961 atctcaaaaa agaagtagag atctattacc aagcgaagga aagataaaaa agccgaggga
     1021 tttcctcgac tctttacata ttatttgttc gttagttctt aataccagcc attgttaaac
     1081 cagaagtttt tagctctgcc aagccacata gtgtgtttac tactatgtgt ccatgtcacc
     1141 atgaatcgcc gaacgattta acgagaaagt atgactttta cgtttatccc aactcaatta
     1201 tgacattttt ttcaaaagtc aatatatctc actttttcaa cgacaagaaa gaggctgcca
     1261 atcgaccaac ctcttattct gaacccatca ctccatcact ttttagcttc attcgctttc
     1321 ttagcgactg caatctggta ttcgacttgg tcaaaccctg tgcctcccaa ggaatttcgt
     1381 ctttgaacag cagttttagg ttgcaagtag acatagatat cctcggtaat gagagaatga
     1441 taggtttgta attcctccaa agtccaatct tgaagatttt tagcagactt gatagagtct
     1501 agcactaatc ttcccacaac ctcatgagct tctctaaatg gcaagccttt ccctgccaaa
     1561 taatctgcca actcagttgc atttgaaaag tccttctctg ttgattcttg cattttttcc
     1621 ttgtttacct gcaaactaga taacatacct gccaacacat cgagggaatt taaaatcgtt
     1681 tcgaccgtat caaacattcc ttccttatct tcttgcaaat ccttattata agctaaaggc
     1741 aaggacttca tgactgtcaa cagtccaaac aaatgcccat aaactctgcc agtctttcct
     1801 cgaatcaact cagccatatc tggatttttc ttttggggca taatagagga acctgttgta
     1861 aacgtatctg acaaggtaat gaattggtac tcaaaactac accaattgat catttcttcg
     1921 caaaaacgac tcatatgcat catgagtatg ctggcatttg atagaaattc taagataaaa
     1981 tcgcggtcac taacagcatc caaagaattt gtataaggtt gcttaaactc cagcaaatcg
     2041 ctagacaatt ggcgatcaat ggggaaagtc gttcctgcca aagctgccgc acctaaaggg
     2101 cataggtctg tatgcttctg gttaaattca aaacgttcac tgtctctttg aaacatattg
     2161 tagtaagcca tcaggtggta ggcaaaacta ataggctggg catgttgcag atgggtatag
     2221 cccggcatga tcgtcgccac atgattttcc gccaagtcta ataaaacacc cttaagatga
     2281 gccagtttat ctaggacata gcctagttgt tccttgagat acaagtgcat atctgtcgca
     2341 acttggtcat ttcgagaacg agccgtatgt aacttccctg caaggggacc aattttttct
     2401 gtcagcaaca cttccatatt catatgaata tcttcgtttg cgatatcaaa gtcaagttga
     2461 cctgcctcta gctcttctaa aagctctttc aagcctactt gaatcttttc tgactcctcc
     2521 aaactcaaaa tgcccgtctg acccaacatc tgaacgtggg ctaaagaacc aatcacatca
     2581 aatttagcta atttctggtc aaaagatata ctcgcaccaa agcgctctac ccagtcttcg
     2641 acagtacctt caaagcgacc accccataat tttgtattct tagccatatc acatccacct
     2701 ctctatccaa tcgtcctatt tggcgctttt ttgaacctca gaatgaacct tggttggaag
     2761 tccccataac tcaataaagc caacagccgc atcttggtca aaggtatctg cactagtata
     2821 cgtcgccaaa ttttcatcgt aaagagaatt tggtgatttc cgagccacga cctgagcgct
     2881 cccccttata cagtttgact tttgcagtcc cattgacaac tttttgagtc tctttaatat
     2941 aagcaatcaa ggcttgtgta gccgaactaa accacaaagc attataaatg agatttgata
     3001 attcattttc tatgattggt ttaaaatgag ccacttctct cacaagcgtc aagtcctcaa
     3061 tttccttatg agctgtcagc agggtcactg cacctggaca ctcatagatt tctcttgatt
     3121 taatcccaac caagcgattt tccacgtggt caatccgtcc aaccccatgt tttccagcta
     3181 tttcattgag tttttgaatc aaatctgcca attttaacac ttctccattg agggaaactg
     3241 gaacaccttc actaaactca atctcaatgt attctggcat atctggagct tgctctggag
     3301 aagttgtgat gccaaaggct tcttctggag cctgattcca tggattttcc aaaataccac
     3361 actcattggc acgtccccaa agattttggt cgacagagta gggattgtca aggtcagcag
     3421 gaacaggcac cccatttttc ttggcataat aaatttcttc ctcccgagac catttccatt
     3481 cacgaactgg agcaatcact tttaaattgg gatccaaggc tgcaatcgat acttcaaaac
     3541 gtacttggtc attcccctta ccggtacaac catgagcaat tgtagtcgct cctatctgat
     3601 gcgctatttc aaccaatttt ttagaaatca gagggcggct caaggcagat accaagggat
     3661 acttttgttc ataataggca tgtgactgat gagccactag cacataatct gtagcaaatt
     3721 cgtccttaac atcaatgaca taagattcta ctgccccaac cttaagagcc ttatcatgga
     3781 tgaaatctaa gtctttccct tcacccacat ccatacaaac tgaaacaaca tcatagtctt
     3841 tctttaacca ggtaatagca actgatgtgt ccaaaccgcc agaataggcc aaaattactt
     3901 tttctttact catgttcttt tccttctttc tatttttcaa tggatgcttt aaaggcatcc
     3961 tcaatgagtt tgtctaattc cccagactcc ttcaactttt gaatagtttt atcgactgcc
     4021 tctttcaatt ccttactatc ttttttcatg gctaccgagt aggaatcatc ttgctctttt
     4081 tcaaaattga ggtctgcgat tgctaaatca ggattatttt ccacaaagcc cttggcaact
     4141 ggttcttcaa agataacggc atccacttgt cctgatttta aatctgtgat taaattccca
     4201 tttttaggca gagatacgag ggaagaattt tgtagcaaat ctttcgccat cgtctcttga
     4261 atcgaacctt tctgggctcc gaccttcttc tgcgctaagt cgttgacaga ctgataagtg
     4321 actaaatcag attttttgac aatgagttta ttttttgcag tatagtaggg aatggaaaag
     4381 tcaaacacct tgctccgttc atctgtctta gaaacacctg atatggcaag atcagctttt
     4441 cctgattgaa cactagccag tacattgtca aaactcatgg gagatagttc caattcgaca
     4501 cctagttctg ttgcgatagc cttggctaat tcgatatctg aacccacaat ctgatttttc
     4561 ccatcaacca ctttttggta ttcaaatgga gcaaaatctg gattgagggc tacaaccaat
     4621 tttcctttgg acttgatggc ttcaattcca gccgattgat tgttactaca agcaaatagt
     4681 agggggagca tcactaaacc aaacatcgtc atcaacacct tcttcatctt attcataaca
     4741 aagcctcctt tatgtttatt ccttttgttt gtataaataa tacacctatc ttcatccttt
     4801 gtcaagccta aattgtaatt tttttcaatt taaaacagaa aaacctagga aaattacata
     4861 aaaatgtcat tcctaggcgt atttatgcta ttcctctctg gaaaatatga gtattcagtc
     4921 agtcaaaaga agctgaacga actcattttc cctcgcccaa ttcaataatc cgatgacatt
     4981 gttgggctac ataagcatcg tgggtcacaa taatgactgt tttcccttct cgattcatct
     5041 ctaagagaaa cttcaagacc aaatctctat tttcaggatc cagcgaacct gtaggttcat
     5101 cggctaaaat cagctggctg ggttttaaga tggctctagc aactgcaatt cgttgttgtt
     5161 cgccaccaga caactcggag accttttgat gcaaagtagc tgacaaacct actctctcta
     5221 aaatctcttc cacctttttg agcttatctt tcttagacaa ttttacatat ttcagcgcca
     5281 gcataagatt gtactcgacc gtttcatcat caatcagggc aaaattttga aacagataag
     5341 agatatgttc acggattatt gtttgcgact tagcagaatt gaccgctaga tttgtctgac
     5401 caaaaatctc ataccgtccg ctataatcac catctatcaa acccaataaa tttaacaagg
     5461 tcgacttccc actaccactc ttaccaacaa tggcaaccaa atccccctga tcaatcctga
     5521 gagacaagcg atccaaaatc actttccccc caatggtttt ggtaatattt tccaactcaa
     5581 tcataagatg ccccctttca ataactctac tagacttctt ttctccatcc tagaagccaa
     5641 ggctagcaca aatagtatat ccaaacatgt aaagcctgca aacagcagga gtggcaagaa
     5701 cgcatgggca aagaaaatca agactagaag agggagacta tagcccagca agagcagaac
     5761 gaggagagga cggtagcgat cgaccagttt ccaccccata aacttcttgg taataatatc
     5821 cctgcgcttc aataagaaag ttattactag taagaagtag gaaatcatca tgctaaagag
     5881 accaaacaaa gcaaagagta ggttaaaatt ccgaacagca tctcgataag aatccacttt
     5941 ctcttgttga atggcttgaa tagatgaaaa ttttaaataa tttccatctg acaatttctc
     6001 aactaactct gtaatctctt tttgatgttg aaccgtattt tcaattttaa tcggattatt
     6061 taagccagtt gttgacaggg aggctttctc atcccacatc atatcagaat cattgaccaa
     6121 gctaataatt ggattggaga gattttcctt tcgcttatca ctatatggga aaaatgacca
     6181 atctccttca taataggcaa tctcgacatc catctcctct atcgttcgtt tttgctgctc
     6241 ttcatacttc atcgaatgaa aggcaattaa cttccccaag agctgatttt tatcttcttc
     6301 acctttcgta cttgctggca tcaaaataac ttttttaata ccagtatctg gtagcttgaa
     6361 tcccttgctc tttagaaaat tgcgattggc atagtaaaca tccaccgtat ctgttaactg
     6421 atattgctga atctgttctg attggacaaa attttttaca ggaagactgc tactctgcac
     6481 atagcccgcc tgcgtttttt ctactaaatc ctgataaaat cgatagaaat aatccaatgc
     6541 cttccctgag cctgccaact gctcttgcca caagttgtca ttgagttgga aagtttctaa
     6601 ggtcagataa tttccttggc tgatccactg tttctggtaa tccaactctt tgttttcttg
     6661 ctccaaactt tttcctaccc caactagcaa gactgtcagt aaaatggtag ttccaatttt
     6721 catcaaataa ttaaagagta gaccaaattt gaaagatgaa aaacctttca gcagagagct
     6781 gattgtcatt ttttggatta agaggtaagt caaccaactg ataaagagat aaagctgcaa
     6841 cagcaaaaaa tgagacaacc acagcatagg aaacaaatct tttggcttat aatcaagcaa
     6901 gaaaaacacg cctagattga tcacaagagc cccacctagg aggaggtaaa ggttgccttt
     6961 tacaacatca gctaaaacag ccctatcttg aaaaccaagt aatttttgta ccccaactcg
     7021 tttcatctcc atcatcggtt gatacactgt cactaacaca agaagcaaaa tagccaagac
     7081 aaaaacaatg gcagataaaa gcaaatctcg atttatgact tccactgcac ttttgtaggt
     7141 cggctctagc aaggtagcct ggtctatctt gaaaaaatcg ctccatttct gtacaatcct
     7201 atccttgtcc atctcttgtg tagaagttat cgtatagcga ccatttaaac tacgagatgt
     7261 atccttgata taggtttgaa aagtcataag ctgaataggt ttggctttta gaaaggtcgg
     7321 aatcgtacca agtttattgg aaatttcttt attactatag actccttcac catctgtggt
     7381 aaaatcaaga gaagaaatcc caaactcttg gtaggggaag gtatctttat caaaaacacc
     7441 agacttgacc acctcatcgc cattatctac tttgataata gagaccttgt cttggcttgc
     7501 aacctcttca aaaaactgga gaatcgtttc tgtcggttta gtgacatctt tcaagtacaa
     7561 gtcaaatgaa taagttgtct tgcccatctc ctgaatccgc actatttctc gtgtaacatt
     7621 tacattcagg acaaaaaacg tagtacacag caagagcagg agcatacaaa gatgactgat
     7681 ttttttcata gagatacctc ccgtgaagaa actccctctc caaaagggga gggagaattt
     7741 taattaaaat ccatagaaat attccattcc cgttggtggg aatttgaaga ttgaagcccg
     7801 agcccacgcc tcagcttctg cacgatcctt agagaattta tttccatctt taacagttgc
     7861 tgtatgagag cgagtagaat gtaaataatc agaataacca aaatttcctg accatcctac
     7921 tccatagttc cattgaccac catcaaccca tactgccaaa acacttgtag aagcaagtat
     7981 actacatacc attaaagatg caacactaat tttctcatga tttatttctc ctattatcga
     8041 gagctattcc gtagaaacag ctatagtaat ttatcaacaa gtatcgtagt ccattggact
     8101 caccttcctt ctatctcatt tcatttttaa acaacgaact tactcttaac taggagaata
     8161 gttctcttct gtatcaggtc tttgaacaac cagagtagta ttaatcacac tctaactatt
     8221 actcaagaat cagcccaata aacaatatgg atagcgcttt caaattttta aactctatcc
     8281 catcctttta tctatattat gagcgaaaat ataataactg tcaagtaatc gaagtgaatt
     8341 ttataaaaaa aattacaagc caaatttgca aagtttacaa taagccgcta gggcaatcgt
     8401 ctatcagaat gtccgtttat ttgtcaataa tccgagaaaa tcttgcaacg cttagaagtc
     8461 tataaaaact atcaacattt atatgacttg cgaatagcaa tcctgctcaa cctttccaca
     8521 ctctatctat acaatcaaga taaaaacatg tgtaagcaaa tctgctatac tttactggag
     8581 gacgccaaga ataagaaaag ctacgatagg ctggctatct gctatgtccg tattgggatt
     8641 tgtacagacg attctaaact tatccaaaaa gggttctccc ttctggagct aaccgaggaa
     8701 acttctatgc tgtctcatct caaaaaagaa gtagagatct attaccaagc gaaggaaaga
     8761 taaaaaagcc gagggatttc ctcgactctt tacatattat ttgttcgtta gttcttaata
     8821 ccagccgttg ttaagccaga agtttttagc agctgtccat gaaccgtaac gtcctgcaac
     8881 gtaggcatct gctacacgtt cttggttttc agctgagtag tcaccgttca ggtatgaatc
     8941 tgttaattgg taacgtccga tataacgtcc atttgtagct gtatagctac cacctgattc
     9001 tttttgagca atccattctt tggcttctgc ttcagatccg cttacagttg aaacaactgt
     9061 ttcacttact actggagttt ctgctactgg agctgaaaca gtttcatctt gagcggctgg
     9121 tgccgcataa gtagctggcg ctggtgttgc aacaggcgct acagggccat cgataaccaa
     9181 ctcttgacca acataaatca aatgaatgtt atcaatgtgg ttgttttctg ccaatttttc
     9241 aactgttgtg ttgtgagttt cagcgatttc tgaaagtgta tcaccttctt taacagtgta
     9301 agttgatgat tcttgagcag atacaaatga tggagcaaat actgcaaaca aggcagctac
     9361 tcctgcaaga gttgctttaa tctttttagt tattgatttc atattcgaaa attctccttc
     9421 tttctatagt tctatcatac cctttaaata ttaccgtttc ttgacacttt tatgtagaaa
     9481 tattacaaaa ttatttttta tttccctaaa taaggtgttt tttgtcataa aagatactat
     9541 ttttatgcta tttgaggtat tagaaaggtt ttcatccaca attaaagcca agcccctcat
     9601 tctttgatat aatagagata agaattttta taaggggaaa ctgatgaaat cacttcgttt
     9661 tcaatctgtc tttgatatca tcggaccagt tatgattggc ccatctagta gccatactgc
     9721 tggtgctgtt cgtattggga agattgtctc ttccattttt gatgatactc cgacagaagt
     9781 tgaattccaa ctttttaact catttgccaa gacctatcgt ggtcacggaa cagacctagc
     9841 ccttgttgca ggtattttgg gaatggatac agatgatcct gaaattccaa atagtctgga
     9901 aattgcccac aagcgtggta tcaagattgt ctggaccatt cagaaagaca gcaatgcccc
     9961 tcaccctaac accactaaaa ttactgttaa aaatgcccac aagaccatca gcgtgactgg
    10021 tatttctatc ggtggaggaa atattcaggt aaccgaactc aatggttttg ccgtctctct
    10081 caatatgaat acaccgacta tcatcatcgt tcatcaagat attccaggta tgattgccct
    10141 tgttacagag gcgctttccc gctatggtat caatatcgcc cagatgaatg tcactcgtga
    10201 aaaagctggt gaaaaagcca ttatgattat cgaagttgac agtcgcaact gtgatgaggc
    10261 tatcgaagaa attcgaaaaa tccctcatct ccacaatgtc aatttcttta aataggagga
    10321 agcatgtttt attctatcaa agaattggtc gagcaagcag atctggactt tcaaggaaat
    10381 gtcgcagaac tcatgattac aacagagttt gaattgaccg gtcgcgaacg tgaagaagtc
    10441 ttccttctca tggaacgcaa tctggaagtt atgaaagcct ctgtccaact tggcctcaat
    10501 gaaaataaat ctcgtagtgg cctgacaggt ggagatgctg ccaaattgga tcactacatc
    10561 gaaaacggaa aaactttatc agattacacg attctctctg ctgcccgaaa tgctatcgca
    10621 gtcaatgaac acaatgctaa aatgggcttg gtctgcgcta ctcctaccgc aggaagtgcc
    10681 ggttgtctcc cttccgttct cactgctgct attgaaaaat tagacctcag ccacgagcaa
    10741 caattggatt tcctctttgc tgctggtgcc tttggactag tcatcgcaaa caatgcctcc
    10801 atctcaggtg ctgagggtgg gtgtcaagct gaagttggtt cagcctctgc tatgagtgct
    10861 gccgccttga ctctggctgc aggtggaaca ccttatcagg ccagtcaagc tattgccttt
    10921 gtcattaaaa atatgctagg cctcatctgt gaccctgttg caggtttggt cgaagttccc
    10981 tgtgtcaaac gtaatgccat gggagctagc tttgctttca tcgcagcaga catggccttg
    11041 gcaggtatag aatctaaaat ccctgtggat gaagtgatcg atgccatgta ccaagtagga
    11101 gcaagcatgc caactgcctt tcgtgaaaca gctgaaggtg gactcgctac cacccctact
    11161 ggtcgtcgcc tccaaaaaga aattttcgga gaataagctt atcaaatagg agaaaccatg
    11221 acctctatca cagcgatttt tttcgatttg gatggaaccc tcgttgatag ttctatcggg
    11281 attcacaatg cctttaccta tacctttaaa gagctggggg tgcctagccc tgatgccaaa
    11341 actattcgtg gttttatggg accacctctc gaaagtagtt ttgcgacctg cctgtccaaa
    11401 gaccaaattt ctgaagccat gcagatatat cgttcttact ataaggcaaa aggcatctat
    11461 gaagctcaac tctttcctca gattatagac ttgcttgagg agttatcgag cagttatcca
    11521 ctctacatca ccacgacaaa gaatacatct actgctcaag acatggccaa aaacttggaa
    11581 atccatcatt tctttgatgg catttatggt tctagccctg aagcacccca taaggcagat
    11641 gtcattcacc aagccttaca gacacatcaa ctagcaccag aacaagccat catcatcgga
    11701 gataccaagt ttgatatgct gggagctcga gaaacaggta ttcagaaatt ggccgtcact
    11761 tggggatttg gagagcaagc agatctacta aactatcaac ctgattatat cgctcacaaa
    11821 cccttagaag ttttggcgta ttttcaatag catagactgg gcgacaactc tatttcagaa
    11881 gaaaatgaag taatactcaa tgaaaatcaa agagcaaact aggaaactag ctgcgggctg
    11941 ctcaaaacac tgttttaagg ttgtagatgg aactgacgaa gtcagctcaa aacactgttt
    12001 tgaggttgtg gatagaactg acgaagtcag taacatatac atacggcaag gcgacgttga
    12061 cgtggtttga agagattttc gaagagtata agtctctaca taataaaacg catattacca
    12121 aggttgtgta acttgatact atgcgttttt cttttttatt caatgtgggt tgtttgatta
    12181 gcaaaggcga taatagcttg cttcaactca tctccactga gagtgcggaa ctctccaatc
    12241 ttttgattga tggattctaa aggcatgaca ttaaccttac caacgaaaat cttatccata
    12301 acttgattat caacgagttc ggttgatacc aagagttctt cgtagagttt ttcacctggg
    12361 cggattccaa cttcaacgat tggaatttca ctttcagtgt ggccacttag aagcaccatc
    12421 ttcttggcca agtcataaat cttgactggt ttgcccatat caaggataaa gacttcccca
    12481 tctttggcat aagcaccagc atggataacc agacggctag cttctggaat ggtcataaag
    12541 taacgggtca tgcggaagtc tgtcaccgtt acaggcccac cttcagcaat ctgacgttca
    12601 aagactggaa tgacactacc acggctacca agaacattcc caaaacgaac tgcacagtag
    12661 gttgattggc tacgttggtt aaagccagtg acaatcaact ccgccacgcg cttggttgct
    12721 cccataacat ttggtggatt gactgcctta tctgtcgaaa tcataaccat cttagacact
    12781 ttagcttcat caacagcctt agcaacattg taagttccac ggatattgtt tttgaaggct
    12841 tcttttggat tgcgctccat cataggaaca tgcttgtggg ctgccgcatg ataaacaata
    12901 gcaggtttgt actgctcaaa gacttgcaac aaacgatcat agtcttgaat gtccgcaatc
    12961 acaggtacat aatcaatccc ttggaactta cgaatcaatt catgataaac aaggtagatt
    13021 gagttttccc catgaccgag taagacaatg cgttcaggat tgaagcgact aacttgacga
    13081 cagatttcag aaccgattga acctccagct cctgtgacta agatggtctt acctgtcagt
    13141 tctgcaccca gacgcgattc gtcaagacgg atttcctgac gacccaaaag gtccgtaata
    13201 tcaatttttt ggaagccagt acctgcttgg tgaaggccct gaacaacagt ttcaacctta
    13261 ggcatcttgt aacatttgac acccagctta ttacacatct gcaagatacg ctcatattct
    13321 gacggatcca gcgacggaat cgcaacgatg acacgctcga tttgatggcg tttggctaat
    13381 tcaggcagat tgtcataaga gcccaaaaca ggaataccac caagtttttg accctttttc
    13441 ttagaatcct tatccaaaat accgaccagt tctaattcac tggttggatg ttggtaacta
    13501 tccataaaga gagccccacc atcaccggca ccaatcaaga aggtccgacg gtgttctcca
    13561 tcaccactac cttttttgcg tctggagtag attaactgcc aagtaatccg tggcaataaa
    13621 atcaagaagg tactcaacaa gataaagaga atgatgaaac ggatggagaa gagtggcaag
    13681 aaggcataac agatactata tgacaagaca ctgctagcag tcacaccaaa aaagattttc
    13741 atgaaatccg taatcttgct gtaacgacta atgctcgcgt tcaaccccca aaaaccaatc
    13801 atcaattgat agaacaagaa ggccaaactc gtatagataa tgtagtcaac aggtgctgga
    13861 ttaatcagcc cataaaataa aatataagat acaattatgg aaaccaccat actgaaaata
    13921 tcaaatattc cccagaaaac ctgcttttgt tgtttattta aaatttccac cagatcaatc
    13981 acataatctg ttagtttttt attcatagaa atctactcct ccttaaaaga gttttactat
    14041 attgttatta tagcatattt ttatttattt ggcttatttt atccaaataa attattgttc
    14101 tcttgtactc cttcttccat ttaaatcctc taattaaacc tacaatcgtc ccaaggccat
    14161 aagcaaagtg aatggaaaat aaaagaaagg gcatcacaat tagaaatcca tttttatgtt
    14221 ttaataaagt cagcaaagtg agtaatgaca aaagtagaaa ataggcacct aatagtaaag
    14281 ttatgaatac gaatgtgatc ggtaacaatg ctagactaaa cacaagactc aaaacaaata
    14341 aacaaggaac atagtgaaat aatgataaac acttaaactg aacatgactt gtcaagccaa
    14401 tccacaaacc atttgaatac ttttgatgca gcattttctt gaatgttggt cgaatatact
    14461 gataagatag aatacttggg ctatagcgga ttttataacc atgttctcga attctataat
    14521 gaatatcatt atcttcagtt cggccaagtt gctcatttac taaaccaacc ttctggaaaa
    14581 cctctcgttt atacatccca tgaaaaatag aagaaacata tctatcctcg gaactatttc
    14641 gataattggc aatgctactg ccaaacatat tttcctcaac aagatgcaag gtctctgccc
    14701 attttccttt tccttcgaca atcgtcggtc taggcccccc acagacaaat tcaccttgtt
    14761 gaataatagc cacattgttc attacaaaac tctcagtaac ttttgaatga gcatcaattt
    14821 ttaaaataag gtcccctaca gaatgtttaa ctcccaggtt aaaaccacta gcttgatttt
    14881 tcttaggatt gttatacaat ctaattgagt taaactctgt atcttccttt ataaattgct
    14941 gaatgatagc tgtggtccca tctgtggaca tagcatttat aaatagaatt tcaacattct
    15001 ctttaggata ggtttgattt tttaagtctt caattagacc aggcagatat ttttcttcat
    15061 tgtatgcact gattacaatg gatacgagaa gactttcaac attttgagtc actactactc
    15121 tcctgaattt ttccttccac cattctatca taattttcaa gactttccca tttttatctt
    15181 gaaagccaga aaccttactt gacattatag tgaaaaagcc ttaaaataag ctgttattaa
    15241 aatcagctag aagaggtatt atatgaaaaa gcaatcactc ttttttgttc caggtattat
    15301 cctgattggt gtttccttgc gaactccttt tactgtttta cccattattt tgggaaatat
    15361 ttcgcaaggt ctggaggtag aagttagttc gcttggtgtc ttgaccagcc tgcctctcct
    15421 tatgtttacc ctcttttcac cattttctac ccgactggct cagaaaatcg gcttggagca
    15481 tctcttcacc tacagcctct tcttcttgac catcggctca ctcattcgac taatcaatct
    15541 gcccctgctc tatctaggaa ccttgatggt tggggcaagt gtcgcagtca tcaatgtcct
    15601 gcttcctagt cttatccaag ccaatcaacc aaagaaaatt ggttttctga ccaccttata
    15661 tgtaacgtct atggggattg caacggctct ggcttcctat ctagctgtgc ccattacaca
    15721 agccagttct tggaaaggac ttatcctcct cctcacgtta ctctgtctag caactttttt
    15781 ggtctggctc ccaaatcacc gctataatca tagactagct ccacaaacca aacaaaaaag
    15841 tcaaataaag gtcatgcgta ataaacaggt ttgggcaatt attatctttt caggttttca
    15901 atccttgatc ttttacaccg tcatgacctg gttacctacc atgtctatcc atgcaggtct
    15961 atccagtcac gaagctggct tgctgacttc tatcttatct ctgattagca ttcctttttc
    16021 aatgaccatc ccaagcctga caaccagttt atctactcgc aaccgtcagc tcatgctcac
    16081 tctggtttca ctagctggtg tggtcggcat ttccatgctc tttttcccaa tcaataattt
    16141 catttactgg cttaccatcc atctcctcat cggaaccgca accagcgccc tcttccctta
    16201 tctcatggtc aacttttcac tcaagacaag cgcccctgaa aagacagccc aattgtccgg
    16261 cctatctcaa acaggaggct atatcctagc agcctttggg ccaaccctct ttggttacag
    16321 ttttgacctg ttccactctt gggtaccagc tgtagctgcc ctcttgctca tctatatcct
    16381 gatgactgtg gccctcttta cagtggacag agcggataag atcctttaaa cttctagttt
    16441 tagctagaag ttttttatat ataaaaattt tacacatttc tcttgacagg gctttctttt
    16501 agatgtacaa tgtatgtgta gaaaaattat atataaaaat cttacacatt agaaaaggag
    16561 gtttcccatg tactttccaa catcctctgc cttgattgaa tttctcatct tggctgtact
    16621 ggagcagggt gattcttatg gttatgagat tagccaaacc attaagctga tcgctaatat
    16681 caaagaatcc acactctatc ccattctcaa aaaattggaa ggcaatagct ttctgacaac
    16741 ctattctaga gagttccaag gtcgcatgcg caaatactac tccttgacaa acggtggtat
    16801 agagcagctc ttgaccctaa aagatgaatg gacactctat acagacacca tcaatggcat
    16861 catagaaggg agtatccgcc atgacaagaa ctgaatacct gactcagcta gaactctatc
    16921 tcaagaaact acctgaagct gaccgtatcg aagccatgga ctatttcaga gagctctttg
    16981 acgatgctgg agtcgaagga gaagaagaac tcatcgctag tttgggaact cccaaagaag
    17041 cggcccacga agttctatcc aatcttctcg ataaaaaaat caatgaagca cccgctcaaa
    17101 aaaataaccg acaaatttta catatcgcct tgttagccct ccttgcagca cctatcggca
    17161 ttcctctggg aatcgccatc ctcgtgaccc tgttcgcaat ccttgtagcc gctttgactg
    17221 tcattctggc tttctttgca gtttccatac tgggtatcat cggcggattc ctatttttag
    17281 ttgaaagttt cactgtcctc gcccaagcca aatcagcctt tatcttgatt tttggttctg
    17341 gtttactggc tatcggtgct tcttcgctag ttttacttgg catttcctat gtaactcgct
    17401 tcttcggtct actcattgtt cgtctggtac aatttgttct taaaaaagga aagagaggta
    17461 accagcatgc gtaaatggac aaaaggattt ctcatctttg gtgtggtgac taccgttatc
    17521 ggctttatcc tgctttttgt aggtatccaa tctgacggga ttaagagcct actttccatg
    17581 tccaaagaac ctgtctatga tagccgtacg gaaaagctaa cctttggcaa ggaagtcgaa
    17641 aacctagaaa ttactctcca ccaacacacg ctcaccatca cagactcttt cgatgatcaa
    17701 atccacattt cttaccatcc atctctttct gctcaccatg atcttatcac caatcagaac
    17761 gatagaactc tgagtctcac tgataagaaa ctgtctgaaa ctccgtttct ctcttctgga
    17821 attggtggga ttcttcatat cgcaagtagc tactctagtc gttttgaaga agttattctc
    17881 cgactaccaa aagggagaac tctaaaaggg atcaacatct cagccaatcg cggacaaacc
    17941 accatcataa atgctagcct tgaaaatgcg accctcaata caaacagcta tatcctccga
    18001 attgaaggaa gtcgtatcaa aaacagtaaa ctcacaacac ccaatatcgt taatatcttt
    18061 gatacagttc ttacagatag tcagctagag tcaacagaga atcacttcca cgctgaaaat
    18121 atccaagtcc atggtaaggt tgaactgact gccaaagatt atctcagaat catcctagac
    18181 cagaaagaaa gccaacgaat taactgggac atctcaagca actatggttc tatcttccaa
    18241 ttcacaagag aaaagcctga atcaagaggt acggaattaa gcaaccctta caaaactgaa
    18301 aaaaccgatg tcaaggatca actcattgcg agatctgatg ataatattga tctaatatcc
    18361 acaccaagca gacgttgaca aaaacgctta catctgctac tatggaagca tatttatcta
    18421 actaaaaagg aggttctacc atgaaacaag aatggtttga aagtaataat tttgtaaaaa
    18481 caacaagcaa gaacaagcct gaagagcaag ctcaagaggt tgcagacaag gctgaagaaa
    18541 cgatagccga tttcgataca ccaattgaaa aaaatactca gttagaggag gaagtctctc
    18601 aagctgaagt cgaactggaa agccagcaag aagagaaaat tgaaactcct gaaggcggtg
    18661 aagcgagaac agaaatagaa gaaaagaagg catctaattc tactgaagaa gagccagacc
    18721 tttctaaaga aacagaaaaa gtcactatag ctgaagagag ccaagaagct cttcctcagc
    18781 aaaaagcaac cacgaaagag ccacttctta tcagtaaatc tttagaaagt ccttatatcc
    18841 ccgaccaagc tccaaaatct agggataaat ggaaagagca agtgcttgat ttttggtctt
    18901 ggctagtgga agcgatcaaa tctcctacaa gtaagttgga aacaagtatc acacacagtt
    18961 acacagcctt tctcttgctc attctgtttt ctgcatcttc ctttttcttt agtatctatc
    19021 acatcaaaca tgcttactat ggacatatag caagcattaa cagtcgcttc cctgagcagc
    19081 tagctccttt aactcttttt tctatcatct ctatcctagt agcgacaaca ctcttcttct
    19141 tttcattcct cttgggtagt ttcgttgtga gacgatttat ccaccaggaa aaggactgga
    19201 cgctagacaa ggttctccaa caatatagtc aactcttggc aattccaatc ttcctcactg
    19261 ctattgctag tttctttgct ttctttgata gcctacgatt tacagctctc ttgtgtgtga
    19321 ttagcattgg aatcattctg cttgccagtc tccatatcat tacaagacca agtcaagcaa
    19381 gtgaaaccga ctccttctat caattattct tgtctgtcct tgtgaacgga gttattatcc
    19441 tcctcttctt tgtagctgaa gtggcactga ttggagatta tcttcgtatc ttggcctttc
    19501 tttaaaattc aatcctgtca tccttggcag gattttttct atgtcaaaaa agcaagtcga
    19561 ttgacctgct tctgctttac ttttcttgtg ctaatgtttt aaaatcttgt actaggatgg
    19621 gttgtatttc tatttgattg gcgtctagtt ggtggtaaaa agatgggggt aatacctcta
    19681 agaattttgc atagtccaag cgagctatgg attcatagtc ttctggttta gagtcgtctc
    19741 ctgtgatttg aaccaagtca atctcccact ggacttcctt atccaccaat tgctcaatat
    19801 aggctgcagg aacaaacggt tctctcggta aaactgtctt gactccttga gctagatggt
    19861 aaacgccatg cacttttcct tctccatcct gatagaagga aactctcgca aagtaggcat
    19921 ctgtctcttg aaccgcacgc acacgcgctt caaactgagc caagccatct gcctctaaaa
    19981 agcttttcaa gttctcacga ctaaagaaaa taggattaaa aattccttgg caaatcgtaa
    20041 aacgagctac agggtctgct agataggctt gaatacttgc ttggaaataa gcttcaaaat
    20101 caaaaccatc tagcccttca atatcagtcc ctgtataagc aagcaaagca tctaaaaatg
    20161 acttgataat ctgccagtct gtctttgtta gtaggtcagg aagatcgaca cggtaagcct
    20221 tttgaccatc agcataactg accttaaaaa gaaattgtga ttgccccact atcgcacact
    20281 caatatactc gagacgatta agaggctgac ggagatagac tacatcatag ctatgtgact
    20341 ccaaaccgtc tagcaaggcc aagatcgact tggcattcaa aatctcttgt tctcctaaaa
    20401 tgctctgttt atttggaata aaaaatgttt tcgccataac tggactcctt tgaaatcgtt
    20461 tacatacagt ataccttatt tttctgaaag atacaaaaac aaactttaga tttttgagta
    20521 caaagcatag aaaaacagcc cctgaggact gtttgatctt atacagtagc tgcctgatcc
    20581 aagctttcac cgatagcggc taggcgctca atcacttcag cttgtgtcaa ttcatttttt
    20641 gaaacatagc ggttacgtgg gtgaacacgg cactcgtgtg agcatccacg aaggtacttg
    20701 tcttcatttt cttctgatgt caagatacga cggttacaga agggatttcc acagttgaca
    20761 taacgttcac atggtgttcc atcaaaccag tctttcccta cgatggttgg gttgacatgg
    20821 ttgacatcaa cggcgatacg ctcgtcaaat acgtacattt ttccatccca aagctcacct
    20881 tgaacttctg ggtctttacc gtaagttgcg attcctccgt gcaattggcc gacatctttg
    20941 tagccttcac ggaccatcca gcctgagaat ttctcacagc gaacgccacc tgtacagtaa
    21001 accacgacac gcttgtccat gaatttttcc ttgttatcac ggacccattg tggtaactca
    21061 cggaagttgc gaatatctgg gcgaatagct ccacggaaat gtcctaggtc gtactcataa
    21121 tcgttacgtg tgtcaaggac aacggtatct ttatcaagaa gcgcttcttt gaactctttt
    21181 ggagacaagt aagcacctgt tgtttcaagt gggttgatgt cattgtcaaa gtcgttgtct
    21241 tccaaaccaa ggtggacaat ttctttcttg tagcgaacaa acatcttctt gaaggcttgt
    21301 tcattttctt cgtcaatctt gaaccagagt tcttccattc ctggaaggct gtgaacgtag
    21361 tccatgtatt tttgagttgt ttcatagtca cctgaaactg ttccgttaat tccctcgtca
    21421 gcgactagga tacggccttt aaggccgatt gatttacaga aagccaagtg gtctgcagca
    21481 aattgctctg cattttcaat tggagtataa aggtagtaaa gtaagacacg aatatctttt
    21541 gccataagat ttgttctctt ttctattctt aaattttcta aattttttat ttatctacac
    21601 tagtatacct gcttgagaaa acgaatgcaa tttatttgac tggaaattgt agagggttct
    21661 cgaaatttcc tgaatggtta aaataaggac aagagaaaac atggatatct atatccttgt
    21721 gccaaaaaaa ccactgccct ccccagacca acctgaggaa agcagtgatt cttattttag
    21781 gagttaggaa tgaatacacg aaatcaattt agctgattat tttttgtttt tcaagaattc
    21841 atcgtattgt ttttgcattt cgttcaatac tttttcgtag gcaccttcag atttcaattt
    21901 ttccatcaat tctggaatcg ctttatctgg gtctacagta ccagtgttga tagctgtatc
    21961 aaattgttgc attgtgttag caatagctga gatttcagat ttcacattgt cagtattgaa
    22021 gataaatcca agcgctggag attctttagc ttctgccaat tctttcttag aattttcgat
    22081 ttgttggtct gtaacgtttt cgttgatgta aaggatccag ttgttaccag tgttccatcc
    22141 acccatgtga gtgtttcctt tgtagccatc aagaacgcga acacggtttt ctttaccttc
    22201 aattttttcc cagttcttgc cttctggacc gtaaacaaga ccgttcaaga gttctggatt
    22261 cgtattcaag aggttcaaga tttccattga tttttctttg ttcttagagt tgtttgagat
    22321 gacaaagtta gcaacttgtg ttgtttggtt tttcttgatg aagttagtaa ttggtttgat
    22381 ttggatatct ttgttggcaa cacgtgaaag caagctgtta ccgtagtcag ctggtcctac
    22441 tgtttcttca cgaacgaacc aagtatcttg ttgaaggtca aaggaagtat cgcttgttgc
    22501 gacatctttt ggaatgtagc cagcttcata gaatttgtga agagtcttca agtgttcttt
    22561 gaaacgaggc acttcgtaac ggtttacaac tttagtagta tcgccttcaa ggtcaataac
    22621 gaatggaagc ccgtttgcta ctgggtagtc aaagttatca gatgggatga aaactttacc
    22681 aatagcaaat ggtactacgt ctggagcttt ttctttaatt tgtttcaaga ctggctcaag
    22741 agtttcgtaa gaagtaacac ctgaaatatc gataccatat ttggcaagga gagttccgtt
    22801 gaaggcaaag ttttgagatg atgcaacgtt ggctgcaact ggaacagcgt aaattttacc
    22861 gtttacagta ttacccttga tgtaagctgg gtcaagtgcc ttgtaaaggt ctttaccttc
    22921 ttttttgtac aattctgtca agtcagcata agcacctttt tgagcattta caatatagtt
    22981 atctgcaaag gcgatatcat agttttcacc agatgatgtg ataactgaca ttttcttacc
    23041 atagtcaccc cagccaaggt attggatatc caatttagca ccaacttttt cttcgatgat
    23101 tttgttggca tttgctaaca attcatccaa gttatctggt ttgtcaccga tttggtacat
    23161 tttgataaca ggtttgtcac ctgaatcagc agcttttttg ctgttacctg tcaagttgcc
    23221 acaagcagca agacctgcag ccagagcgac tacactagca gatgcaaaag catatttttt
    23281 ccagtttttc atgataaaaa ctcctttttt tatttttaaa cttataaaca atgtaatgat
    23341 cttatactca atgaaaatca aagagcaaac tagaaaacta gccgcaggct gctcaaagca
    23401 ctgttttgag gttgtagata agactgacga agtcagttac atatatctac ggcaaggcga
    23461 cgttgacgcg gtttgaattt gattttcgaa gagtattaac ttcacacaag ggaagttggg
    23521 aactgagaaa tgttcttttt caataagcac tattctttca caccaccgat agtcaaacct
    23581 tttacaaagt agcgttggaa gaatggatat aaaatcgcga ttggaagggt tgcaaccaca
    23641 accatggcca tacgacctgt ttctttcggt agagcaactc ccagttgacc agtcaagccg
    23701 accgctttgg caatgtagtc catattttgt tggatttgca tgagcaaata ttgcaatgga
    23761 tacaagttat cactcttgat gtaaagaagg gcgttgaacc agtcattcca gaaaccaaga
    23821 gctgtcaaaa gcgtgatggt tgcgatacct ggtagtgaca atggcaaaca gatttggaag
    23881 aaaatccggg cctcactggc accatcgata cgagccgatt cgagaatggc ttctggaatg
    23941 gtcttcttga agaaggaacg catcaagatg atattgaatg gtgagagaag cattggaaca
    24001 atcaaggccc aaacagtgtc cccaagctga agtacacggg tcaccatgat ataacctggt
    24061 accaaaccag cgttgaacaa catactgaga aggacgaaga tggtaaaaaa tctgcgatac
    24121 ttaaaggttg tccgtgaaat agcgtaggca taggttgttg tgataaagac atttgtcaat
    24181 gtcccaacta cggttacaaa gacagagatg aagagggctt gtaggatttt atccttaaac
    24241 tgtgccaaaa actcaaaacc gtctaagcca aattgggatg ggaagaagct atagccgtat
    24301 tggaggaggc ttttctcgtc tgtcactgaa ataatgataa cgaatacaaa aggtaggata
    24361 caagagaggg caatcaaacc cgaaatgata ctgaagaaga tatctgcttt cttactgaag
    24421 gagtgaatgc cgacattatc aattttttct tttttaattt tcttttttgc catattctcc
    24481 tcctttctag aacaaagctg agtttggatc gactcgtctt gcaagcaagt ttgataggat
    24541 aaccagaatc aaaccaacaa cggattggta aagaccggct gctgcagcca taccgatatc
    24601 tgctgtctga gtcaaaccat taaagacata tacgtccaaa acgttggtta cattgtaaag
    24661 ctgaccagca ttgtgtggga tttgatagaa gagaccgaag tctgcgcgga agatatttcc
    24721 gactgcaagg atggtcaata cagttacaag cggagtcaac tgaggaatgg ttacgttgcg
    24781 aatacgttgc cacttgctag ctccgtccac tgtcgctgct tcgtagtagg ttggatcaat
    24841 tcccatgatc gtcgcatagt acatgacact gctatatcca aatcctttcc aaatacctag
    24901 gaaaagtagg agatagggcc agatgcccag gtcagcgtag aaattgactt ctttgagacc
    24961 aagactttcc aatagatgat tgaacacccc tttatcaata tttaggaagg catctgtaaa
    25021 gaaactgatg ataacccaag acaagaagta agggaacaac atagaagttt gaaaaatctt
    25081 caccattctc ttagaacgga gctcgctgag gataatggcg atccctacag atacaactaa
    25141 acctagaaag ataaagccaa gattgtagag gacagtattt cgtgtgataa taaaggcgtc
    25201 tcttgaacta aataagaatc taaaattatc gagtccgacc catttactat ttatgatact
    25261 atctatgaaa ccattactgg tcatgtggta gtctttgaag gcaaccacgt tcccaaatac
    25321 tggaatgtaa aagaatagaa tcaaccagag tgcccctggc aaaaccatca agagaaagat
    25381 ccagttgtct ctcaatgttt ttgaaaactt tttcataatt tcctcccttt ttattttgat
    25441 atccatctaa aaattctttt ttagactttt gataacgatt acattattag tatactccta
    25501 tttgcaggtt aggttaaact cctaattata gaaaaaactc cacaaattat gtagcagatt
    25561 taaaacttta tcaccactat caaacaaatg tcctaaatca attgtttatt ttatctctat
    25621 tagcccagtg atggcgtcac tctgttataa gcatccaaca acggggtata ctgaaaaatc
    25681 tccagactag ggaactcagc gatagttcct aatctggaga tttttaatat gttattaggc
    25741 gtttgctttc aacttagcaa taacctcttt aagattatca atcaactctg ctgcagtatg
    25801 ctcagagcct ttttcatctg ccaagaacaa aactgctttt tgaagttctt tttgagagtt
    25861 ttcaaggaca tccttatcta ctgtttcaag gtttgagtct ttaagaagtt tacttaattc
    25921 cttggctaat ttcttgagtt tgatttgcag actcatcttc tcctgctgtt tctttgcccg
    25981 ctgtttgtcc tccatcctta gttgctgact ggctttcctt aatggactct agggaagcaa
    26041 tggcatcttt gactgtttgc aagatatcac gtaaaccttg ctctgtcaaa ctatcatctg
    26101 taagagcttt cttagcctct gccaaaacca gacgtgctga atctgtggta ggattcgata
    26161 cacctgtcaa tgatctcaaa agattttcta aggtttgagt ctgcttacta atactagact
    26221 aaaatcaaaa agcattacat aacagtgata tgaaatcaac taaagaagaa atccaaacca
    26281 tcaaaacact tttaaaagac tctcgtacag ctaaatatca taaacgcctt caaatcgttc
    26341 tatagtaaaa tgaaacaaga acaggacaaa tcgatcagga cag
//
LOCUS       DAVLVF010000021        26180 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVLVF010000021 DAVLVF010000000
VERSION     DAVLVF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.021"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            381..2534
                     /locus_tag="VYN66_000875"
     CDS             381..2534
                     /locus_tag="VYN66_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1494930.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKANMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2547..3896
                     /locus_tag="VYN66_000876"
     CDS             2547..3896
                     /locus_tag="VYN66_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV1494931.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4066..4773
                     /locus_tag="VYN66_000877"
     CDS             4066..4773
                     /locus_tag="VYN66_000877"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV1494932.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            4830..8555
                     /locus_tag="VYN66_000878"
     CDS             4830..8555
                     /locus_tag="VYN66_000878"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV1494933.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDINGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANNTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8648..10090
                     /locus_tag="VYN66_000879"
     CDS             8648..10090
                     /locus_tag="VYN66_000879"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV1494934.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10127..11149
                     /locus_tag="VYN66_000880"
     CDS             10127..11149
                     /locus_tag="VYN66_000880"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV1494935.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11146..11691
                     /gene="purN"
                     /locus_tag="VYN66_000881"
     CDS             11146..11691
                     /gene="purN"
                     /locus_tag="VYN66_000881"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV1494936.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11775..12284
                     /locus_tag="VYN66_000882"
     CDS             11775..12284
                     /locus_tag="VYN66_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1494937.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12309..13856
                     /gene="purH"
                     /locus_tag="VYN66_000883"
     CDS             12309..13856
                     /gene="purH"
                     /locus_tag="VYN66_000883"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV1494938.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQEFIEAADKYGLTMVFT
                     GVRHFRH"
     gene            13978..15240
                     /gene="purD"
                     /locus_tag="VYN66_000884"
     CDS             13978..15240
                     /gene="purD"
                     /locus_tag="VYN66_000884"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV1494939.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHYKLIDFAKANDIAWTFIGPDDALAAGIVDDFHAAGLKAFGPTRLAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15633..16121
                     /gene="purE"
                     /locus_tag="VYN66_000885"
     CDS             15633..16121
                     /gene="purE"
                     /locus_tag="VYN66_000885"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV1494940.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16108..17181
                     /gene="purK"
                     /locus_tag="VYN66_000886"
     CDS             16108..17181
                     /gene="purK"
                     /locus_tag="VYN66_000886"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV1494941.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLISNEPDSVGEF"
     gene            17204..19591
                     /locus_tag="VYN66_000887"
     CDS             17204..19591
                     /locus_tag="VYN66_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1494942.1"
                     /translation="MFEKIKGINIKSGIFEDETKLELFEGNFEGTNPVQNDRASLLFG
                     RNGSGKSTIARGINQLKNGEIGTDRVSFIDKNNNNIVLSDTERKSIFVFNEHYVDQKV
                     KIAQEGLDTIVILGEQVDIDEELDRLRTQLSESQIESQDYYAEYEEYLDEKNEKSPDF
                     WKKEMTDSLKGVGNWAERDREIKGNRAASPVHNNTFQNFVDLQPILDKNELEVEFNNK
                     KARYFSIRDSAVTINNELSLPDINFDSNELSTLLSEKIEEPELNSRDKYLLTLLSDST
                     KGERHLREVKDFFEDEHQKKCPFCTQSVSEDVKVELTNGITKLLSRAVEEHQSALRGK
                     KIDEINQDFSGYEQIDPILIQSYQNSINALNAKFNEINSIIDKKIDNPYNIVELPNIS
                     FSQELSQAEHDIEKINQAIIKHNSEISGIQKLKVDLLQINNELAFYEIQDAYKKFQEK
                     TNKKAICENNYNNSSKRVKDYEKQISDLEDKKLNIDIAVDEINKSLNYIFFSKNRLAI
                     QNQNGKYYLLSRGKSVVPSRVSVGERNALALCYFFTEIIQQRELADAYSHEYFIVIDD
                     PISSFDMENKVGITSYLKYCLTRFFKGNSNTRVLLMTHDKQTIYDFDIFLKEIMESCK
                     EEEGGQKSKYKKLELVSGKLQEFKTSTHDYTELLEIVFGYALGNSTPTSESFVGNAMR
                     KILEAYGSFNYKKGIAELTTDPLIVEKIDKEYRTYFENLMYRLVLNGESHFKDPVKTL
                     SIDFFDTISDEERKKTARDLLVLLYLLDDLHVLKHLEGVSNAENRLEQWKCEILE"
     gene            19764..21062
                     /gene="purB"
                     /locus_tag="VYN66_000888"
     CDS             19764..21062
                     /gene="purB"
                     /locus_tag="VYN66_000888"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV1494943.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEINEIFNPIYYTKRVDDIFERLGLGD"
     gene            complement(21117..25055)
                     /gene="strH"
                     /locus_tag="VYN66_000889"
     CDS             complement(21117..25055)
                     /gene="strH"
                     /locus_tag="VYN66_000889"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1494944.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKEEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETETGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPRLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(25370..26086)
                     /locus_tag="VYN66_000890"
     CDS             complement(25370..26086)
                     /locus_tag="VYN66_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1494945.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
BASE COUNT         7414 a         5007 c         6075 g         7684 t
ORIGIN      
        1 gaaatactaa aatttttttc tttacttaat aatgatatga aatcatttcg gttttgttaa
       61 cttgtaattc ttatattttc taggtagaac gtttgttctg cctatttttt tatccaaaaa
      121 gtgcagttgg gagggagata ggctcatttg ggaaggaagt ccagtttttg tttagtgatt
      181 ggggtaagat agttgttatc agatgagttt atactcttcg aaaatcaaat tcaaaccacg
      241 tcaacgtcgc cttgccgtat atatgtgact gacttcgtca gtcctatcta caacctcaaa
      301 acagtgtttt gagcagcctg cggctagttt cctagtttgc tctttgattt tcattgagta
      361 ttagggaaaa ggagatgaat atgaaatttg ggaaacgtca ctatcgtccg caagtggatc
      421 agatggactg cggtgtagct tcattagcca tggtttttgg ctactatggt agttattatt
      481 ttttggctca cttgcgagaa ttggctaaga cgaccatgga tgggacgacg gctttgggct
      541 tggtcaaggt ggcagaggag attggttttg agacgcgagc cattaaggcg aatatgacgc
      601 tttttgactt gccggatttg acttttcctt ttgttgccca tgtgcttaag gaagggaaat
      661 tgctccacta ctatgtggtg actgggcagg ataaggatag cattcatatt gccgatccag
      721 atcccggggt gaagttgact aaactgccac gtgagcgttt tgaggaagaa tggacaggag
      781 tgactctttt tatggcacct agtccagact ataagcctca taaggaacaa aaaaatggtc
      841 tgctctcttt tatccctata ttagtgaagc agcgtggctt gattgccaat atcgttttgg
      901 caacactctt ggtaaccgtg attaacattg tgggttctta ttatctgcag tctatcattg
      961 atacctatgt gccagatcag atgcgttcga cactagggat tatttctatt gggctagtca
     1021 tcgtctacat cctccagcaa atcttgtctt acgctcagga gtatctcttg cttgttttgg
     1081 ggcaacgctt gtcgattgac gtgattttgt cctatatcaa gcatgttttt cacctcccta
     1141 tgtccttctt tgcgacacgc aggacagggg agatcgtgtc tcgttttaca gatgctaaca
     1201 gtatcatcga tgcgctggct tcgaccatcc tttcgatttt cctagatgtg tcaacggttg
     1261 tcattatttc ccttgttcta ttttcacaaa ataccaatct ctttttcatg actttattgg
     1321 cgcttcctat ctacacagtg attatctttg cctttatgaa gccgtttgaa aagatgaatc
     1381 gggataccat ggaagccaat gcggttctgt cttcttctat cattgaggac atcaacggta
     1441 ttgagactat caagtccttg accagtgaaa gtcagcgtta ccaaaaaatt gacaaggaat
     1501 ttgtggatta tctgaagaaa tcctttacct atagtcgagc agagagtcag caaaaggctc
     1561 tgaaaaaggt tgcccatctc ttgcttaatg tcggcattct ctggatgggg gctgttctgg
     1621 tcatggatgg caagatgagt ttggggcagt tgattaccta taataccttg ctggtttact
     1681 ttaccaatcc tttggaaaat atcatcaatc tgcaaaccaa gcttcagaca gcgcaggttg
     1741 ccaataaccg tctaaatgaa gtgtatctag tagcttctga gtttgaggag aagaaaacag
     1801 ttgaggattt gagcttgatg aagggagata tgaccttcaa gcaggttcat tacaagtatg
     1861 gctatggtcg agacgtcttg tcggatatca atttaaccgt tccccaaggg tctaaggtgg
     1921 cttttgtggg gatttcaggg tcaggtaaga cgactttggc caagatgatg gttaattttt
     1981 acgacccaag tcaaggggag attagtctgg gtggtgtcaa tctcaatcag attgataaaa
     2041 aagccctgcg ccagtacatc aactatctgc ctcaacagcc ctatgtcttt aacggaacga
     2101 ttttggagaa tcttcttttg ggagccaagg aggggacgac acaggaagat atcttacggg
     2161 cggtcgaatt ggcagagatt cgagaggata tcgagcgcat gccactgaat tatcagacag
     2221 aattgacttc ggatggggca gggatttcag gtggtcaacg tcagagaatc gctttggcgc
     2281 gtgctctctt gacagatgcg ccggtcttga ttttggatga ggcgactagc agtttggata
     2341 ttttgacaga gaagcggatt gtcgataatc tcattgcttt ggacaagacc ttgattttca
     2401 ttgctcaccg cttgactatt gctgagcgga cagagaaggt ggttgtcttg gatcagggca
     2461 agattgtcga agaaggaaag catgctgatt tgcttgcaca gggaggcttt tacgcccatt
     2521 tggtcaatag ctagaaagag gagaggatga aaccagaatt tttagaaagt gcggagtttt
     2581 ataatcgtcg ttaccataat ttttccagta gtgtgattgt acccatggcc cttttgctcg
     2641 tgtttttact tggctttgca actgttgcag agaaggagat gagtttgtcc actagagcta
     2701 ctgtcgaacc cagtcgtatc cttgcaaata tccagtcaac tagcaacaat cgtattcttg
     2761 tcaatcattt ggaagaaaat aagctggtta agaaggggga tcttttggtt caataccaag
     2821 aaggggcaga gggtgtccaa gcggagtcct atgccagtca gttggacatg ctaaaggatc
     2881 aaaaaaagca attggagtat ctgcaaaaga gcctgcaaga aggggagaac cactttccag
     2941 aggaggataa gtttggctac caagccacct ttcgcgacta catcagtcaa gcaggcagtc
     3001 ttagggctag tacatcgcaa caaaatgaga ccatcgcgtc ccagaatgca gcagctagcc
     3061 aaacccaagc cgaaatcggc aacctcatca gtcaaacaga ggctaaaatt cgcgattacc
     3121 agacagctaa gtcagctatt gaaacaggta cttccttggc cggtcagaat ctagcctact
     3181 ctctttacca gtcctacaag tctcagggcg aggaaaatcc ccaaactaag gttcaggcag
     3241 ttgcacaggt tgaagcacag atttctcagt tagaatctag tcttgctact taccgtgtcc
     3301 agtatgcagg ttcaggtacc cagcaagcct atgcgtcagg gttaagcagt caattggaat
     3361 cccttaaatc ccaacacttg gcaaaggttg gtcaggaatt gacccttcta gcccagaaaa
     3421 tcttggaggc agagtcaggt aagaaggtac agggaaatct tttagacaag gggaaaatta
     3481 cggcaagtga ggatggggtg cttcatctta atcctgagac cagtgattct agcatggttg
     3541 cagaaggtgc cctactagcc caactttatc catctttgga aagagaaggg aaagccaaac
     3601 tcacagccta tctaagttca aaagatgtag caagaatcaa ggtcggtgat tctgttcgct
     3661 atactacgac tcatgatgcc gggaatcaac tttttctaga ttctactatt acaagtattg
     3721 atgcgacagc tactaagact gagaaaggga atttctttaa aatcgaggcg gagactaatc
     3781 taacttcgga gcaggctgaa aaacttaggt acggggtgga aggccgtcta cagatgatta
     3841 cgggcaagaa aagttatcta cgttattatt tggatcaatt tttgaacaaa gagtaatgtt
     3901 cgtgttttta gagttaaata atttttaaac tgtgagaaag attcttcttg cagttttttc
     3961 tttacaattt ttgaaaaaca tctgctattt attcggttaa attcttgtgt tttttggttt
     4021 tttgtggtaa aatgtgctca agtaatacga aaggcgaact ttaaaatgtc aaaacaattg
     4081 atctattcgg gaaaagctaa agatatctat acaactgagg atgaaaatct tattatttca
     4141 acttacaagg accaggcgac tgctttcaat ggtgtcaaga aggagcagat tgcaggtaag
     4201 ggagtcttga ataatcagat ttcatctttt atttttgaga aattaaatgc ggctggtgtg
     4261 gcgactcact ttgtggagaa actttcagac acggaacaac tcaataaaaa ggttaagatt
     4321 attcctttgg aagtcgtgct ccgcaactat actgctggtt ccttttcaaa acgttttggt
     4381 gtggatgagg gaatcgcctt ggagactccg attgtcgaat tttactacaa aaatgatgat
     4441 ttggatgatc catttatcaa tgatgagcat gtaaaattcc tacagattgc ggatgaccag
     4501 cagattgcct acttgaagga agaaacgcgt cgtatcaatg aactattgaa agtctggttt
     4561 gctgagattg ggcttaaatt gattgacttt aagctagagt tcggttttga caaggatggc
     4621 aagattatct tggcagacga attttcacca gataactgcc gcttgtggga tgctgatggc
     4681 aaccacatgg ataaggatgt tttccgtaga ggattgggag aactaaccga cgtttatgag
     4741 attgtttggg aaaagttgca gggtttaaaa taacaacctc aaggctgttt gggaatattg
     4801 caagagctga aataaaggaa taagaattga tggataaacg tatttttgtt gaaaaaaagg
     4861 ctgattttca ggtcaagtca gagagtttgg ttagagagct ccagcacaac ttgggactgt
     4921 caagcttgaa aagtattcgt attgtgcaag tatatgatgt atttgacttg gttgaggact
     4981 tgtttgcacc tgcagagaag cacattttct ctgagcaggt aaccgaccat gttttagatg
     5041 aagtatctgt gcaggcggat cttgctaact atgctttctt tgccattgaa agtctaccag
     5101 ggcagtttga ccagcgtgca gcttcgtcac aggaagcctt gcttttgttg ggaagttcga
     5161 gtgacgtgac agtcaacaca gcccaacttt acttggtgaa taaagatatt gatgcgactg
     5221 agttggaagc tgtcaaaaac tacctgctca atccagttga ttctcgtttc aaggatatca
     5281 cgatagggat tgccaagcag gagttttcag agtcagacaa gaccattcca aaattgcctt
     5341 tctttgaaag ctatacagca gaagactttg ctcgctacaa ggccgaacaa gggatggcca
     5401 tggaagtgga tgatttgctc tttatccaag actactttaa gtcaatcggg cgcgtgccaa
     5461 ctgagactga actcaaggtt ttggacactt actggtctga ccactgccgt catacgactt
     5521 ttgagacaga gttgaaacac atcgactttt cagcttctaa atttcaaaag caattgcagt
     5581 caacctatga caagtatatt gccatgcgcg aggaattagg tcggtctgaa aaaccacaaa
     5641 ccttgatgga tatggcgact attttcggtc gttatgagcg tgctaatgga cgattggatg
     5701 atatggaagt ctctgacgaa atcaatgcct gctcagttga aattgaagtg gacgttgatg
     5761 gtgtcaagga accttggctc ctcatgttta aaaacgaaac tcacaaccat ccaacagaaa
     5821 ttgagccatt tggtggagcg gctacctgta tcggtggagc tattcgtgat ccgttgtcag
     5881 gccgttccta tgtttaccaa gccatgcgta tttcaggtgc tggtgatatt acagcaccga
     5941 tttcggaaac tcgtgctggg aaattgccac aacaagtcat ttctaaaaca gcaactcatg
     6001 gttattcttc atatggtaac cagattgggc ttgccacaac ctacgttcgt gaatacttcc
     6061 acccaggctt tgtagctaaa cgtatggaac ttggtgccgt tgttggtgcg gctcccaagg
     6121 gcaatgttgt ccgtgaaaaa cctgaagcag gtgatgtgat catccttctc ggaggtaaaa
     6181 caggtcgtga tggtgtcggt ggtgcgacgg gctcttctaa ggttcaaaca gttgagtctg
     6241 tagagactgc tggtgctgag gttcaaaaag gaaatgccat cgaagaacgc aagattcagc
     6301 gcctcttccg taatggcaat gtcactcgtc tgatcaagaa gtccaatgac tttggggcag
     6361 gcggtgtctg tgtggctatc ggtgaattgg cagacggtct tgaaatcgac ctcaacaagg
     6421 tgcctcttaa ataccagggc ttgaatggta cagaaattgc catctctgaa tcacaagaac
     6481 ggatggcggt cgtggttcgt cctgaagatg tggatacctt cgttgccgaa tgtaacaaag
     6541 aaaatattga tgctgttgtg gtggcgacag taactgaaaa accaaatctt gtcatgcact
     6601 ggaatggtga gacaatcgtt gacttggagc gtcgtttcct tgacatcaat ggtgtgcgcg
     6661 tggttgtcga tgccaaagtt gtggacaagg atgtcaaact cccagaagag cgtcaaacat
     6721 ctgctgaaac actggaatca gataccctta cggttctatc tgacctcaac catgcaagtc
     6781 aaaaaggatt acagactatc tttgactgct ctgttggacg ctcaacggtt aatcacccac
     6841 ttggtggtcg ttaccaactc acaccaactg aggcatctgt gcagaaattg ccagttcaac
     6901 acggtgtgac tcatactgcg tcggtcattg ctcaaggttt caacccatat gtagctgaat
     6961 ggtctccata ccacggtgct gcttatgcgg ttatcgaagc aactgctcgt ttggtggctg
     7021 ctggtgccaa ctggtccaag gctcgtttct cttaccaaga atatttcgag cgtatggaca
     7081 agcaagcaga gcgttttggt cagccagtag ccgctcttct aggctctatt gaagctcaga
     7141 ttcaacttgg cttgccatct atcggtggta aggactccat gtctggtacc tttgaagaat
     7201 tgaccgttcc gccaaccttg gttgcctttg gggtgacgac ggcagatagc cgtaaggtgc
     7261 tctctccaga atttaaagct gttggggaaa atatctacta catcccaggt caagccctct
     7321 ctgcagagat tgattttgac ttgattaaga aaaattttgc tcagtttgaa gcaagccaag
     7381 ctgaccataa agtgacatct gcatcagctg tcaaatacgg tggtgtagtt gaaagtttgg
     7441 ctcttgctac ctttggaaac tatattggtg cagaggtgac cttgcctgaa cttaaaacag
     7501 ctttgacagc tcaattaggc ggctttgtct tcacatctcc tgaagaaatt gctggagtag
     7561 agaagattgg acaaacgaaa gcagacttta cactgactgt caacggtgtg aagctagatg
     7621 gacacaagct tgacagtgca tttcaaggga cattggaaga agtttaccca acagaattta
     7681 cccaagcgaa agaactagaa gaagtaccag ctgtggcatc agatgttgtg attaaagcca
     7741 aagaaaaggt tgaaaaacct gtggtttaca tcccagtctt tccaggaacc aactcagaat
     7801 atgattcagc taaggccttc gaaaaagaag gtgcagaggt caatttggtg ccattcgtga
     7861 ccttgaatga agaagctatt gtcaagtcag ttgaaactat ggttgacaat atcgacaaga
     7921 ctaatattct cttctttgct ggtggattct cggctgcgga tgaaccagat ggttcagcta
     7981 agtttatcgt caatatcctg cttaatgaaa aagtgcgtgt ggctattgat agctttatcg
     8041 cccgtggtgg tttgattatc ggtatttgta atggattcca agccttagtc aaatcgggtc
     8101 tcctacccta cggaaacttt gaagatgcta acaatactag cccaaccctc ttctacaatg
     8161 atgccaacca acacgtggcc aagatggtgg aaactcgcat tgccaatacc aactcaccat
     8221 ggttggctgg tgtgcaagtg ggcgatatcc acgctattcc tgtttcgcac ggtgaaggga
     8281 agtttgtcgt gacggctgag gaatttgcag agctccgtga caatggacaa attttcagcc
     8341 aatacgttga ctttaacggt aaaccaagta tggattctaa gtacaatccg aatggttctg
     8401 tccatgccat cgaaggaatt accagcaaga atggtcaaat catcggtaag atgggccact
     8461 cagaacgtta tgaggatggt cttttccaaa atatcccagg caataaagac caacacctgt
     8521 tcgcatcagc ggttaaacat ttcactggaa aataagactt acagattttc taatagatag
     8581 tatcagtaat gtaaaagtca tgtaaatcta gctcttgatg attacaaatg aaaattaggt
     8641 ataaaaaatg acatacgaag taaaatctct taatgaagaa tgtggtgttt tcggtatttg
     8701 gggacatcca gatgctgcta agttgaccta ttttggactc cacagtcttc aacaccgtgg
     8761 tcaggagggg gcaggaatcc tctccaatga tcaaggacaa ctgaagcgcc atcgtgacat
     8821 ggggctttta tcagaagttt tcagaaatcc agccaatttg gataaattga caggagctgg
     8881 tgcgattggg catgtgcgtt atgcgactgc tggcgaagct tctgttgata acatccagcc
     8941 cttcctcttc cgttttcacg atatgcagtt tggcttggct cataatggaa atctgaccaa
     9001 tgcagcctct ctcaagaaag aactggaaca aagaggagca attttcagcg cgacttcgga
     9061 ctctgaaatc ttggctcacc tcattcgtcg cagtcataat cctagcctga tgggcaaaat
     9121 caaggaagcg ctcagccttg tcaaaggtgg ttttgcctat atcttgctgt ttgaggacaa
     9181 gttgattgcg gctcttgacc ctaatggctt tcgtccgctt tctatcggga aaatggccaa
     9241 cggagcggtg gttgtttcct ctgaaacctg tgcttttgag gtcattggtg ccgagtggat
     9301 tcgtgatttg aagccaggtg agattgtgat cattgatgac gagggcattc agtatgacag
     9361 ctatacagat gatactcagt tggcgatttg ttctatggag tatatctact ttgctcgccc
     9421 tgattctaat atccacggtg tcaatgtcca tacggcacgt aagagaatgg gagcgcaatt
     9481 ggcgcgagaa tttaagcatg aggcagatat tgtagttggt gtgcccaatt cttccctaag
     9541 cgcggctatg ggatttgcgg aagaatcagg cttaccaaat gaaatgggtc tgatcaaaaa
     9601 ccaatacacc cagcgaactt ttatccaacc gactcaagaa ttgcgggagc aaggagtgcg
     9661 gatgaaactg tctgctgttt cgggtgttgt caaaggcaaa cgtgtggtca tggtggatga
     9721 ttccattgta cgtggaacaa cctctcgtcg tatcgttcag ctcttgaaag aagcgggtgc
     9781 gactgaggtt cacgttgcca ttggaagtcc tgcactagcg tatccatgtt tctacgggat
     9841 tgatatccag acccgtcagg agctgattgc agccaatcat acggtcgaag aaactcgcca
     9901 aatcattggt gcggacagtc tgacttatct ttcaattgat ggcttgattg agtcgattgg
     9961 tatcgaaaca gatgcgccga acggtggtct ctgtgtcgct tactttgacg gtgactaccc
    10021 aacgcctctc tatgactacg aagaagacta tcgtagaagt ttggaagaaa agaccagttt
    10081 ttacaagtag gcgacagatt ctccattaaa gaaaaggaaa aaacaaatga caaataaaaa
    10141 tgcatatgcc cagtcgggtg tggatgttga agcgggttat gaagttgttg aacggattaa
    10201 aaagcacgtg gcccgtacgg agcgtgcagg tgtcatggga gctcttggtg gctttggtgg
    10261 tatgtttgac ctttccaaga ctggggttaa agaacccgtc ttgatttcag ggactgacgg
    10321 tgtcggaacc aagctcatgt tggctatcaa gtacgacaag cacgatacca tcgggcagga
    10381 ctgtgtggcc atgtgtgtca acgacatcat tgctgcaggt gcggaacccc tctattttct
    10441 cgactacgta gcgacaggga agaatgaacc agctaagcta gaacaagtgg ttgctggtgt
    10501 ggcagaaggt tgtgtgcagg ctggtgctgc cctcatcggt ggggaaacgg ctgaaatgcc
    10561 gggcatgtac ggcgaagacg actatgactt ggctggtttt gcggtcggtg tggctgaaaa
    10621 atctcaaatc attgacggtt caaaggtggt agagggagat gttcttctcg gacttgcttc
    10681 aagtgggatt cactcaaatg gttactcttt ggttcgtcgt gtctttgcgg attacacagg
    10741 tgaggaagtc ctaccagaat tggaaggcaa gaaacttaag gaagttctac ttgagccgac
    10801 tcgtatctat gtcaaggctg tcttgccgct catcaaagaa gagttggtca acggcattgc
    10861 ccacatcaca ggtggtggct ttatcgaaaa tgtccctcgt atgtttgcag atgacctagc
    10921 tgctgaaatt gatgaaagca aagttccagt gcttccaatt ttcaaagccc ttgaaaaata
    10981 tggtcagatt aaacacgaag aaatgtttga aatcttcaat atgggtgtgg gacttatgtt
    11041 ggcggtcagc cctgaaaatg tagagcgtgt aaaagaattg ttggatgaag cagtctatga
    11101 aattggtcgc atcgtcaaga aagaaaacga aagtgtcatt atcaaatgaa aaaaatagcg
    11161 gtttttgcct ctggtaatgg ctcaaatttt caggtgattg ccgaagaatt tccagtggag
    11221 tttgtctttt cagaccatcg tgatgcctat gtgcttgagc gtgcaaagca gctcggcgtt
    11281 ctgtcctatg cttttgaact caaggagttt gagagcaaga cagactacga agcagccctt
    11341 gtcgaactct tggaagaaca ccagattgac ttggtttgcc tagcaggcta catgaaaatc
    11401 gttggaccaa ccttattgtc ggcttatgaa ggtcggattg tcaacattca tccagcctac
    11461 ttgccagaat ttccaggagc tcatgggatt gaggatgctt ggaatgctgg cgtgggtcag
    11521 tctggtgtga ccattcactg ggtggattcg ggtgtggata caggccaggt catcaaacag
    11581 gttcgtgtgc cacgactagc tgatgatacc attgacagat ttgaagctcg catccatgaa
    11641 gcagagtaca ggttgtatcc ggaagtagtg aaggctctat ttacagattg actttttgat
    11701 gattcatatg atatctttga ttttaaattg gagtcagtgt ttgttgaaga cggcttcaaa
    11761 cggaggtatt tgtaatgtta gaatctaaaa aaacaactcg atatgtattt tatgtctatc
    11821 tgatgttatt aacttgggga atcttattta agtttgaaac aaatcctgaa tttatagcat
    11881 ttttcttagc tccaaggtat atcaattgga ttccattttc agaaccacta atagtcgatg
    11941 gaaaaattgt ttttgctgaa atgttattta atctgatttc ctttattcca ttaggtgttt
    12001 gtttcccttt gataaaaact aatttatcta gtttaagaat agtcgggaca ggtttcttga
    12061 ttagtttatt gtttgagtgc ttacagtata ttttagcaat aggtataaca gatataacgg
    12121 atttgacttt aaatacgcta ggtgtctgtg taggcttact gatttatcaa atttttataa
    12181 gagtgttcaa atcacagact agaaaatgga tcaatatctt aggtatgctt agccttggtt
    12241 ttgcttatct tgttttactg ttactgcatt taattggtgt ttaactaatg attaaaaagg
    12301 agaatataat gactaaacgc gtcttaatca gcgtctcaga caaagcgggc attgttgaat
    12361 ttgcccaaga actcaaaaaa cttggttggg agattatctc aacaggtgga actaaggttg
    12421 cccttgataa tgctggggtg gataccattg ctatcgatga tgtgactggt ttcccagaaa
    12481 tgatggacgg tcgtgtgaag accctccacc caaatatcca cggagggctt ctcgctcgtc
    12541 gtgacttgga tagccacttg gaagcggcta aggacaacaa gattgagctc attgaccttg
    12601 tggtggtcaa cctttaccca tttaaggaaa ctatccttaa accagatgtg acttatgctg
    12661 atgcagttga aaatatcgat attggtgggc cgtctatgct tcgttcagca gcgaaaaatc
    12721 atgccagtgt tacagttgtg gtagatcctg ctgactacgc tgtggttttg gacgaattgg
    12781 cagcaaacgg cgaaacctct tatgaaactc gccaacgttt agcagccaaa gtatttcgtc
    12841 acacagcggc ttatgacgcc ttgattgcag aatacttcac agctcaagtg ggtgaaagca
    12901 agcctgaaaa actcactttg acttatgacc tcaagcaacc aatgcgttac ggtgagaatc
    12961 ctcaacaaga cgcggacttt taccagaaag ctttgcctac agattactct attgcttcag
    13021 ccaaacagct caacgggaaa gaattgtcat ttaataatat ccgtgatgca gatgctgcta
    13081 tccgtatcat ccgtgacttc aaagatagtc caaccgttgt ggctctcaaa cacatgaatc
    13141 catgtggaat tggtcaagct gatgacatcg agactgcttg ggactacgct tatgagtctg
    13201 acccagtatc tatctttggt gggattgtcg tcctcaaccg tgaggtggat gctgcgacag
    13261 ctgagaagat gcacggcgtt ttcctcgaaa tcatcattgc accaagctat acggatgaag
    13321 cgctagctat tttgatcaat aaaaagaaaa acttgcgtat ccttgccttg ccatttaatg
    13381 ctcaagaggc tagcgaagtg gaagcagaat acacaggtgt agtcggtgga cttctcgtgc
    13441 aaaatcaaga cgtggtcaag gaaagcccag ctgactggca agtggtgacc aaacgtcagc
    13501 caactgagac agaagcgact gctcttgagt tcgcttggaa ggctatcaag tacgtcaaat
    13561 caaatggtat tatcgtgacc aacgaccaca tgacacttgg tgttggtcca ggtcaaacca
    13621 accgtgtggc ttctgttcgc cttgccattg accaagccaa agatcgtctg aacggggcgg
    13681 tccttgcttc agatgccttc ttcccatttg cggataacgt ggaagaaatc gccaaagcag
    13741 gaattaaggc catcatccag cccggtggct ctgtccgtga ccaagaattc atcgaagcag
    13801 cggataaata cggcttgact atggtcttta caggtgtgag acattttaga cattaagaag
    13861 ataaaaggga agaaaacagt ttctttcctt ttttggctta aaatgctaac tgaaacaaga
    13921 ttaaaacgaa cttttttgat ataatgttgg taaataattc gcaaaagagg ttgaggaatg
    13981 aaactgcttg ttgtcggttc tggtggtcgt gagcatgcga ttgctaaaaa gttacttgaa
    14041 tcaaaagacg tggaaaaagt ctttgtagct cctgggaatg acggtatgac tctggatggt
    14101 ctggaattgg taaatatatc tatttccgaa cattataaat tgattgactt cgcaaaggcc
    14161 aacgatattg cttggacctt tatcggtcca gatgacgccc ttgcggctgg tatcgtggat
    14221 gatttccatg cagctggtct caaagccttt ggtccgacaa gattggcagc ggagctggag
    14281 tggtccaagg atttcgccaa ggaaatcatg gtcaaatatg gtgtttcgac agcagcctat
    14341 ggcacatttt cagatttcga ggaagcaaag gcctatatcg aaaagcatgg tgcgcctatc
    14401 gtagtcaagg cggatggctt ggcgcttggg aagggtgtcg tcgttgctga gacggttgag
    14461 caagcggtcg aagccgctca tgagatgctt ttggacaata aatttggtga ctcaggtgcg
    14521 cgcgtggtta ttgaggaatt ccttgaagga gaggaatttt cactctttgc ctttgtcaat
    14581 ggtgataagt tctacatcat gccaacggct caggaccaca aacgtgccta cgatggcgac
    14641 aaagggccta acacgggtgg tatgggtgcc tatgcgccag ttccacactt accacagagt
    14701 gtagttgata cagcggttga caccattgtc aagccagttc tagaagggat gattaaagaa
    14761 ggtcgccctt atctgggagt tctttacgca gggcttatcc tgacagctga tggaccgaaa
    14821 gtcattgagt tcaacgctcg gttcggagat ccagaaactc agattatctt gcctcgcttg
    14881 acctctgact ttgctcaaaa tatcacagat atcctggata gcaaggagcc aaatatcatg
    14941 tggacggaca agggtgtgac tctgggtgtg gttgtcgcat ccaagggcta cccgctagac
    15001 tatgaaaggg gcgttgagtt gccagccaag acagaaggcg atgtcatcac ctactatgca
    15061 ggggctaagt ttgcggaaaa tagcagagca ctgctctcaa acggcggacg agtttatatg
    15121 ctcgttacca cagcagatac cgtcaaagaa gcccaagcca gcatctacca agaactatcc
    15181 caacaaaaaa tagaaggact cttctaccga acagatatcg gaagcaaggc aattaagtaa
    15241 agatataaga ataacgcgcc gtagtcgcca aacacgataa tggtcgtcgt ggtgaaaaga
    15301 ccagaacagt gaatgttctg gtcaggggga aacttggaga ccgtaggctc aaagtttagg
    15361 aatgaaaccg aaggtttgct tccgcctcca tcacctaaga ccattatcaa aaagaaaaat
    15421 aaaaattcac aaaatacgtt aatgatcgta tggttagcga gctaatatag aacaatcacc
    15481 gccgttgtga aagaacgatt ggatgataat ccaatcgttc agggaaattg gaagaccttg
    15541 ggtttccaat ttaggcatga gacacctttg gtggctgctg ccgtccctca caagctaagg
    15601 tgattgttga aaaagaggaa aaaggagaag aaatgaaacc agtaatttcc atcatcatgg
    15661 gctcaaaatc cgactgggca accatgcaaa aaacagcaga agtcctagac cgcttcggtg
    15721 tagcctacga aaagaaagtt gtttccgcac accgtacacc agacctcatg ttcaaacatg
    15781 cagaagaagc ccgtagtcgt ggcatcaaga tcatcatcgc aggtgctggt ggcgcagcgc
    15841 atttgccagg catggtagct gccaaaacaa cccttccagt cattggtgtg ccagtcaagt
    15901 ctcgtgctct tagtggagtg gattcactct attctatcgt tcagatgccg ggtggggtgc
    15961 ctgttgcgac catggctatc ggtgaagctg gagcgactaa cgcagctctc tttgccctcc
    16021 gtctcctctc tgtagaagat aagtccattg cggatgcact tgccaacttt gctgaagaac
    16081 aaggaaaaat cgcagaggag tcgtcaaatg agctcatcta aaacaatcgg aattatcggt
    16141 ggcggtcaac tgggtcagat gatggccatt tctgctatct acatgggcca caaggttatc
    16201 gcgctggatc ctgcggcgga ttgcccggcc tctcgcgtgg cggaaatcat tgtggcacct
    16261 tataacgatg tggacgccct ccgtcagttg gcagaccgtt gcgatgtcct cacttatgag
    16321 tttgaaaatg tcgacgctga cggtttggat gccgttatca aggatggaca actccctcaa
    16381 ggaacagatc tgctccgcat ttcgcaaaat cgtatttttg aaaaggactt tttgtcaaac
    16441 aaggctcaag tcactgtggc accctacaag gtcgtgactt ctagcctaga cttggcagat
    16501 atcgacttgt cgaaaaacta tgtcctcaag actgcgactg gtggctacga tggtcatgga
    16561 caaaaggtta ttcgttcaga agcagacttg gaagcagcct atgcgctagc agactcagca
    16621 gactgcgtct tggaagaatt tgtcaacttt gaccttgaga tttctgtcat cgtgtcagga
    16681 aatggcaagg aggtgacgtt tttcccagtt caggaaaata tccaccgcaa caatatcctg
    16741 tctaagacca tcgtaccagc ccgcatttct gaaagtctag tagacaaggc taaagctatg
    16801 gcagtgcgaa tcgcagaaca actcaacttg tctggaactc tctgtgtgga aatgtttgcg
    16861 actgatgatg acgttattgt caatgaaatc gccccacgac cacataactc tgggcactat
    16921 tctattgaag cctgtgattt ctctcagttt gacacccata ttctcggtgt tctcggagca
    16981 ccattaccag tcatcaaact ccatgcgcct gccgtcatgc ttaacgttct cggccagcat
    17041 gttgaggccg ctgaaaaata tgtcacagaa aattcaagcg cccacctcca catgtatggt
    17101 aaaatagaag cgaagcacaa ccgcaagatg ggacatgtga ctttgattag taatgaacca
    17161 gatagtgtgg gagagtttta ggggagaggg gtctaaagaa aaaatgtttg aaaaaattaa
    17221 aggaattaat ataaaatcag gtatttttga agatgaaaca aaattagaac tctttgaagg
    17281 aaattttgag ggaacaaatc cagtacaaaa cgatagggca agtttattgt ttggaaggaa
    17341 tggttcaggg aaaagtacca ttgctcgagg aataaatcaa ttaaaaaatg gtgagattgg
    17401 aacagataga gtatcattta ttgataaaaa taataataat attgtactta gtgatactga
    17461 acgaaaatcg atatttgttt tcaatgaaca ctatgttgat cagaaagtca aaattgctca
    17521 agaagggtta gatacaattg taattttagg tgaacaggtt gatatcgatg aggaactgga
    17581 tagattaaga acgcagttat cagaatcaca aattgaatct caagattatt atgctgaata
    17641 tgaagaatat ttagatgaaa aaaatgaaaa atctccagat ttttggaaaa aggaaatgac
    17701 agattctttg aaaggagtag gcaactgggc cgaaagagat agggagataa aaggaaatcg
    17761 agctgcatct cccgttcata ataatacctt tcaaaatttt gttgatttac aaccaatctt
    17821 agataaaaat gagttagagg ttgaattcaa caataaaaaa gctagatatt tttcaataag
    17881 agattccgca gttactataa ataatgaact atctttacct gatattaatt ttgatagcaa
    17941 tgaattatca actttattgt ctgaaaaaat cgaagaacct gagttgaatt ctagggataa
    18001 gtatttactg acattattat ctgattccac aaaaggtgaa cgtcatttga gagaagttaa
    18061 agatttcttt gaagatgaac atcaaaaaaa atgtcctttc tgtactcaat cagtatcaga
    18121 agatgttaaa gttgaactca cgaatggcat aactaaacta ctatcaagag cagtcgaaga
    18181 acaccaatcc gcattgagag gtaaaaaaat tgatgagatt aatcaagatt tttctggtta
    18241 tgaacagata gatccaatat taattcagtc ttatcaaaat agtattaatg ctttgaatgc
    18301 taaatttaac gaaattaact caataatcga taagaagatt gataatccat ataatatagt
    18361 tgagttacct aatataagct tttctcaaga attaagtcaa gcagagcatg atattgaaaa
    18421 aattaaccaa gcaattatca aacataattc agaaatctcg ggtattcaaa agttaaaagt
    18481 agatctcctt caaataaata atgaattagc tttttatgaa attcaagatg catacaaaaa
    18541 gtttcaagag aaaacaaata aaaaagcaat ttgtgaaaat aattataata acagtagtaa
    18601 acgagtaaag gattatgaga agcagatttc tgatttagaa gacaagaaac ttaatataga
    18661 tattgcagtt gatgaaatta ataaaagttt aaattacata tttttttcaa aaaatcggtt
    18721 agctatacag aatcaaaacg gaaaatacta tctgttatca agaggtaagt ctgtggtccc
    18781 cagtagggtt tccgtagggg aacgaaatgc gctggcatta tgttatttct ttactgagat
    18841 aattcaacag agagagttag cagatgctta tagtcatgaa tattttattg ttattgacga
    18901 tccaatttct agttttgata tggaaaataa agttggaata acctcgtatt taaaatattg
    18961 tttaactaga ttttttaagg ggaatagtaa tactagagtt ttactaatga cccatgataa
    19021 acaaacaata tatgactttg atatcttttt gaaagagata atggaatcct gtaaagaaga
    19081 ggaaggtgga caaaaatcta aatacaagaa gttagaattg gtttctggta aattacaaga
    19141 attcaaaact agtactcacg attatacaga actacttgaa atagtcttcg gttatgcatt
    19201 gggaaatagt acacctactt cggaaagttt tgttgggaat gcaatgagaa aaattttaga
    19261 agcctatggt agttttaatt ataagaaggg aattgccgag ctaacaacag atcctttaat
    19321 tgttgaaaaa attgataaag aatacagaac ttattttgag aatcttatgt atagactggt
    19381 tttaaacggt gaaagtcatt ttaaggatcc ggttaaaact ttgagtattg atttctttga
    19441 taccatttct gatgaagaac gaaaaaaaac agctagagat ttactagttt tgctttatct
    19501 tttagatgat ttacatgttt taaaacattt agagggtgta tcaaatgcgg aaaatagact
    19561 tgaacaatgg aagtgtgaga ttttagaata agagaatcat accatttgac ttattgcaat
    19621 ggttgaagtt tgcatgatag ctgattagag taatcaaagg attattactt cctaatttta
    19681 ttttctacaa accctaattt agcatctaat aatgcaaagt ttcctgagcc tcatagataa
    19741 aaaagatttt taaggagaaa aacatgatca accgttactc tcgccctgag atggcgaata
    19801 tttggagtga agaaaataaa taccgtgctt ggcttgaggt ggaaatcctc tctgacgagg
    19861 catgggctga gttgggggaa atccctaagg aagatgtggc tttgattcgc aagaaggcgg
    19921 actttgacat cgaccgtatt ttggaaattg agcaggagac gcgccacgat gtggtggctt
    19981 tcacgcgtgc ggtttctgag actcttggtg aagagcgcaa gtgggttcac tatgggttaa
    20041 cttctaccga cgtggtggat actgcctatg gttacctcta caagcaggcc aacgacatca
    20101 tccgtcgtga ccttgaaaac ttcactaata tcatcgctga caaggccaag gagcacaagt
    20161 tcaccatcat gatggggcgt actcatggtg tgcacgctga gccgacaact tttggtctta
    20221 aattggcaac ttggtatagc gaaatgaaac gcaatatcga gcgcttcgag catgcggctg
    20281 ctggtgtaga agctggtaag atttctggtg cggttgggaa ctttgccaat atcccaccat
    20341 ttgtagagga gtatgtctgc gataaacttg gcatccgtgc ccaagaaatc tctacacaag
    20401 tccttcctcg tgaccttcac gctgagtact ttgcggttct tgccagcatt gcgacttcaa
    20461 tcgaacgtat ggcgactgag attcgtggtc tacaaaaatc tgagcaacgc gaagtagaag
    20521 agttctttgc taaagggcaa aaagggtctt cagcaatgcc tcacaaacgc aacccaatcg
    20581 gttctgaaaa tatgactggt ctggcgcgtg tcattcgtgg tcacatgatt acggcttatg
    20641 aaaacgtcgc tctctggcac gaacgcgata tttctcactc atcagctgag cgtatcatca
    20701 caccagatac gaccattttg attgactaca tgctcaaccg ttttggaaat atcgtcaaga
    20761 acttgacagt cttcccagaa aatatgatcc gaaacatgaa ctcgactttt ggtcttatct
    20821 ttagccaacg ggctatgttg acattgattg aaaaaggcat gacccgtgag caagcctatg
    20881 acttggtgca accaaaaaca gcctactctt gggacaacca agtagacttt aaaccacttc
    20941 ttgaggcaga ttcagaagta acatcacgtc tcacacaaga agaaatcaat gaaatcttca
    21001 acccaattta ttacaccaaa cgagtggatg atatctttga acgtcttgga ctaggtgatt
    21061 aattaaaaaa taaacagcga gcttcaatct cgctgtttat tttttatcga aaagacttag
    21121 tcttcttttc ttttagtgag tccataggct gctagtgtgg acatgagtcc tgcgactact
    21181 agtcctgcag aatcgtgagt tcctgtttca ggaagttttt tctctgttac cacaggagct
    21241 ggatcttgag gaagagcttt gctttcctca gcaggagcag ttgatggagc tggttggctt
    21301 gggatttcta gtcttggttt ttcttcagca atagcggctt gtccgttttc atcgcctaca
    21361 tgtgttacca tagttccgac ttcgactatt tgagtaacgg cttcctgtgc tacgacacta
    21421 tttacaagtg ttttcacttc cttaccatcg gcagaagtgc tcacagagta gaagttgcta
    21481 cgatgtccat tgacgccctt agtaatgact tgtgtttttc ctttgagtaa gagtggattt
    21541 tcacgagtca ctgtggtaaa tggaatttct tctttttgga tatccagtct aggttttacc
    21601 tcagtagttg gtgcaagacc actttcatca cccttgtgag ttacaggagc gccaacttca
    21661 accacttggt ttataacttc tttggttacc tggctatcaa ggactgtttc tgttgttttt
    21721 ccattttcag tgagtacaga gatgtaatga gttcgttcac ctttgactcc tgctgtgata
    21781 atattttcct gaccagctgg gaggttagga ttttctttct tgataacttc aaatggaatt
    21841 tcttcagttc ttgtgatgag ttctggtctg gtttcaacat tggcagccac ttcattttca
    21901 tccaggcttc ctgaatgagt tgcagctggt ttgaggcctt gaagagcggc ttttaggttg
    21961 gctacaagtg tgtcaagctc agcttgttta ttacggttga ggttgtaatt tagagctgtt
    22021 ttagctgcgt caagggcctc aagactttct ttactatatc cttctaagtt tgtaggaatt
    22081 ttagctaatt cttcgcggag agcattataa ttagcacgga agtagtcttt gttgtggtct
    22141 gcaaaggcag tcatgagttc aaagatttcc tcttccttgt actcagcgct tggtctatct
    22201 gcccagattg aaagcatact tccgactgtt ggaagatcta cttcaggata tttggtagaa
    22261 gctagttgat tgaatggaac tttaccagaa ttttcaactg cttttgaaag agggtaagct
    22321 tcatcttgtt tgtgattgcc tataacataa taccagtcac cgttggtatt caagaattta
    22381 tagcctttgc ttgctaggta ttgaggtgat gcgaggttat atccccacca gcctttagac
    22441 cagtaagaaa tcaagacatc tttgtcaaac tgaacatcgt ccttgtcttc atagtagaag
    22501 ccatcgttga aggccattgg ttgaagccct ctttctttgg ccatagctgc gagggtgttg
    22561 gcatattcgg caaatttgcc atagagttga taccacttga ggtagtacca gccttgggca
    22621 ctagtcgcat cgttggcgta ttcgtcagta ccaaagttga aaatctttgt tttacctgca
    22681 aagaagtcca tgtatttacc gatgagggct tttacaaagt tcatcgcttc ttcgtttttc
    22741 aagtccatag ttgtttttga aactttatca aagtgggctt gaggattttt aatacctaat
    22801 ttttccatgg caaccagcat agcatccatg tgacctggac tgttaatagc tgggatgaga
    22861 ccaatgtcct tagatttagc gtattcaatt agctctgtca cttctgcctg tgttagtgca
    22921 gtaccgtttg gatcgtcgta gtaagcttta gttccttcga taatagcttt tttaacgtca
    22981 tcactagcat aggtttttcc gttggcagta atggtcatat cattgagtag aaagcgaagt
    23041 ccgtcatttc ctagaaggag atggacatca gaatatccga gctcactggc cttgtctacg
    23101 atgcgtttga gctggtccag agtaaagtat ttgcgtccag catcgattga gattaccttg
    23161 tttttggcaa gtttttcaac ctcacgttta gcttcttctt ctttttgagc ttcaggcgtg
    23221 agggtcaagt tgttgacagt ttcttgaagt ttagcaatgg cttgatcaat cgtatcttgt
    23281 tgggcacggc taaggttgct atcgagagag cgaatagctt tttcagcttc ttttacggcc
    23341 gtgacgcttt ctgcagtata acggttcagg tcttttggta cctcgttaag tgcttgctct
    23401 gcagattcat aatcagctgc gaagtattca gcgttggcat ttgcaaaatg acgcatgagt
    23461 ttgaagaggc gtgatggtga ataacgtgca gatggagtgt cagcccaagc agctaccata
    23521 ccaccgatga ttgggatatc agctccttct gtttttggta cagaagtgat tggtgtgttt
    23581 ttaataccat tgagcccctg atcgagattg taccagcctt ggccatcagc gtttcgtcca
    23641 agaacgtagt accaagcatc attggtatta aggatttggt gacctttttc agctagtagt
    23701 ttagaagaag cgacatcgta gcctccccaa ccaccagtcc acatagaaac gatgatgtct
    23761 ttgtcaaaac taccaaagct tgtgtcgcta ttgtagtaga taccgtcgtt aaaggccatt
    23821 ggtttgagac cgtgcgattt tacaatacga gcgaggtcat tggcgtaggc aataaatttt
    23881 tcatagcctt ttacagggta gccttcgttt ggatagtatt tatcagcttg aagcacactc
    23941 caacctttag catctgtcgc atcattggca tattcatcaa gtccgatgtt gaagatttca
    24001 gtctttttcg cgaaataagc agcatacttg tcgataaggg cttttgtaaa agcgacagct
    24061 tgttcgttgt caagatcgac agtacgggct gatttcttcc caaaatagct aaagttaggg
    24121 ttttggattc ccaattcttt catggcattg agaatcgcat ccatgtgtcc aggactattt
    24181 actgtcggaa tgagaccgat acctttatct ttggcatagt taatcagatc tgtcatttga
    24241 ctttctgtta agtgattgcc gtttggatcg ttgtaataat catttgtacc tttttcaatg
    24301 gcgcgtttga catcgtcact ggcataggtc ttgccgttag ctgtgatgct catatcgtcc
    24361 aacatgaaac ggagtccatc atttccgact aataggtgta aatcagtgta gccataatgt
    24421 ttcgctttat cgatgatttc cttgagctgt tctggtgaga aatatttacg tccagcatca
    24481 atagaaacaa ttttcttttt cgctagtttt tcatttacag ttgcagcacg ttcctttcct
    24541 gtctctgttt ccggtttgtc agcctctgct ttcgcttcat cttttttagc tggtttatcc
    24601 ttgtcagtct tgtctgtatt tgactcttta gaatcaacct ctttcgcttc ttccttttta
    24661 gggctagctt cttcttcctt tttattagca gtttcttttt cagcagaagt tggagttacc
    24721 acctctgctt tatcactagg agttgaacta acttcctctt gtggtttttc ttctgttttt
    24781 ggaagactag ctaccttatc agtagctgga gtttctgttt ctacagtttt tggagcttct
    24841 ggttgaagct ctgctttagg tgtttcctca gtccgatttt cggatgattg aggggaatca
    24901 gaaaccgtat ggatggtcgg ttggttttct gtagtagtag gagtaactcc atcggctgca
    24961 acagtctgtg cttggaaggc aaatccaatt agaacagaag ctgctcctac agcgtattta
    25021 cgaatagaaa aacgctgttg tttttcatgt ttcattgcaa aacctcctga ttgcattgtt
    25081 atattgatag cgattatata aatcaacgcc tttattttat ttcttatatt aacgagagtc
    25141 aagaggagat gacaaaaaac tataataagt ataaaaaaat ataaaattta aacttaagat
    25201 ttcagattgg tcgaaaaaat acatatatat atctagtata atttttggtt ctatttctat
    25261 aaaatattcc acaaattata gaatcttcca aaaataggta agcgctacct ttttggtgtg
    25321 gtataataag catagaaaaa gcccgagcga ttaggctcag gctttcttct tagtgatcac
    25381 ggtcacatga gatgaattta atcttgtagt aatcagatcg tttgtaagtt tcactgtatt
    25441 ctaaaacttg gccagttgat tcgagtttgg tgattttagt ttgtaggaca gtagggaatt
    25501 gttcatcgac tccgaggact gaagccgcat gttctggagt tggaaagact atttcgttga
    25561 tttcttcaaa gtgttcatca ttcatgtgaa tgtggtagtc taacttgaaa cgattataga
    25621 tagaactata gtattcaagg tttggataat ttgcgttgat atattgttct gggatgtagg
    25681 atgtatggta gatataaacg acaccgtttg attcgcggat acgttcaatc ttgtagtaga
    25741 attgatcgcc gcgtagaccc aatttttcca agtaaacaag cttgtttccg cgttcaattg
    25801 aaagaacagt taccttatca tctttagcat tgaagagttc aatatctgaa aactctacaa
    25861 gcttgtgttt gcgtgcacgt gaaacgaagg ttccttttcc ttgttggcgg acaatatagc
    25921 catctttggc aaggtcgttt aaggcgcgaa caactgtgat agagctgaca tcgtacattg
    25981 aaatgagttc tgcttcagtg taaaatttat ctccactgct aaactgccca gagatgattt
    26041 tattttttaa ttcgtctttt atgtattgat atttgggaat tgccatattt ttacctctat
    26101 tttccttctc catagaaaat tttaccataa aagcgaaaaa aatagtagtc ttttgaataa
    26161 aaaattagaa taatagtttg
//
LOCUS       DAVLVF010000022        25162 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVLVF010000022 DAVLVF010000000
VERSION     DAVLVF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25162
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.022"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            118..552
                     /locus_tag="VYN66_000891"
     CDS             118..552
                     /locus_tag="VYN66_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1494946.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQESVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            564..1754
                     /locus_tag="VYN66_000892"
     CDS             564..1754
                     /locus_tag="VYN66_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEV1494947.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1765..2256
                     /locus_tag="VYN66_000893"
     CDS             1765..2256
                     /locus_tag="VYN66_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1494948.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            2271..3050
                     /locus_tag="VYN66_000894"
     CDS             2271..3050
                     /locus_tag="VYN66_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1494949.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            3037..3855
                     /locus_tag="VYN66_000895"
     CDS             3037..3855
                     /locus_tag="VYN66_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1494950.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGENQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            3855..4148
                     /locus_tag="VYN66_000896"
     CDS             3855..4148
                     /locus_tag="VYN66_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1494951.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            4170..6071
                     /locus_tag="VYN66_000897"
     CDS             4170..6071
                     /locus_tag="VYN66_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV1494952.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYHFFADKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKFMSFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGMHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            6131..7132
                     /locus_tag="VYN66_000898"
     CDS             6131..7132
                     /locus_tag="VYN66_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1494953.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7242..7577)
                     /locus_tag="VYN66_000899"
     CDS             complement(7242..7577)
                     /locus_tag="VYN66_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494954.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(7588..7830)
                     /locus_tag="VYN66_000900"
     CDS             complement(7588..7830)
                     /locus_tag="VYN66_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494955.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(7846..8040)
                     /locus_tag="VYN66_000901"
     CDS             complement(7846..8040)
                     /locus_tag="VYN66_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1494956.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            8206..9156
                     /gene="rsmH"
                     /locus_tag="VYN66_000902"
     CDS             8206..9156
                     /gene="rsmH"
                     /locus_tag="VYN66_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV1494957.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPEGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            9168..9485
                     /gene="ftsL"
                     /locus_tag="VYN66_000903"
     CDS             9168..9485
                     /gene="ftsL"
                     /locus_tag="VYN66_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV1494958.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            9489..11741
                     /gene="pbpX"
                     /locus_tag="VYN66_000904"
     CDS             9489..11741
                     /gene="pbpX"
                     /locus_tag="VYN66_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEV1494959.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKVEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            11743..12723
                     /locus_tag="VYN66_000905"
     CDS             11743..12723
                     /locus_tag="VYN66_000905"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV1494960.1"
                     /translation="MFISLSAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            13287..15392
                     /locus_tag="VYN66_000906"
     CDS             13287..15392
                     /locus_tag="VYN66_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1494961.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRTTGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(15688..16170)
                     /locus_tag="VYN66_000907"
     CDS             complement(15688..16170)
                     /locus_tag="VYN66_000907"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV1494962.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(16265..17749)
                     /locus_tag="VYN66_000908"
     CDS             complement(16265..17749)
                     /locus_tag="VYN66_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV1494963.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDATRAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            17902..19512
                     /locus_tag="VYN66_000909"
     CDS             17902..19512
                     /locus_tag="VYN66_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV1494964.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVEMTD"
     gene            complement(19643..21073)
                     /locus_tag="VYN66_000910"
                     /pseudo
     CDS             complement(19643..21073)
                     /locus_tag="VYN66_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398391.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            21230..22675
                     /gene="cps4A"
                     /locus_tag="VYN66_000911"
     CDS             21230..22675
                     /gene="cps4A"
                     /locus_tag="VYN66_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEV1494965.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVHNDQ
                     EFSALHRKFLFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIINSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGTDRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            22677..23408
                     /locus_tag="VYN66_000912"
     CDS             22677..23408
                     /locus_tag="VYN66_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEV1494966.1"
                     /translation="MIDIHSHIVFDVDDGPKSIEESKALLREAYNQGVRMIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYREIHAGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVLKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKEFFVDNPRKIIMDQLI"
     gene            23417..24109
                     /gene="cpsC"
                     /locus_tag="VYN66_000913"
     CDS             23417..24109
                     /gene="cpsC"
                     /locus_tag="VYN66_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEV1494967.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQEEKSGLTNQDLQAGSYLVKDYREIILSQDVLEEVISDLKLD
                     LTPKGLANKIKVTVPVDTRIVSVSVNDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGGIGTSVIVLLLELLDTHVKRPEDIEDTLQMT
                     LLGVVPNLSKLK"
     gene            24119..24802
                     /gene="cpsD"
                     /locus_tag="VYN66_000914"
     CDS             24119..24802
                     /gene="cpsD"
                     /locus_tag="VYN66_000914"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HEV1494968.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIARSFARTGYKTLLIDGDTRNSVISGVFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVIQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTAIGEVNKRDVQKAKQQLEQTEKLFLGVVLNKLDISVDKYGVY
                     GSYGNYGKK"
BASE COUNT         7576 a         4398 c         5645 g         7543 t
ORIGIN      
        1 aaaaagataa tgaaagcgct aatatttttt taaaaaaact attgacaaga taaaaataaa
       61 ggattacaat ggggaatata aagtaaaccg gtaaacctaa acaagaaagg agaaaagatg
      121 aaaattgtac ttgtagggca tggacatttt gctacaggga tttatagttc tttacaattg
      181 attgcaggca atcaagaaag tgtggaggcg attgactttg tggaaggaat gtcagcagat
      241 gaactcaagc aaaaaatctt acttgcaatt tcaaatgaag aagaagtttt aatcctaagt
      301 gatctcttgg gaggatcgcc attcaaggtt tcttctacca taatgggaga aaatccagcc
      361 aaaacaatga atgttctctc gggtttgaac ttagccatgt taatggaagc agtctttgct
      421 agaatggctc atagctttga tgaggttgtt aataaatcag tagtggcggc ccagggcgga
      481 gtcgtaaatg gtaaagaatt gttttcaacg gatgcagagg aagaggaaga agatttcgaa
      541 tcgggtattt aaagggtaaa agaatgataa aaaaggttac gattgaaaaa ataaaatcgc
      601 ctgagcgctt cttagaagta ccacttctga cgaaagaaga agtcggccag gcaatcgata
      661 aggttattcg gcagttagaa ctcaaccttg actatttcaa ggaagatttc cctacgccag
      721 ctacctttga taatgtctat ccaatcatgg ataacacgga atggaccaat ggtttctgga
      781 caggagaact gtggttggct tatgaataca gtcaacagga tgcatttaaa aacatcgctc
      841 ataaaaatgt tctttctttc ctggatcgtg tcaataagag agtagaattg gatcaccatg
      901 atctcggctt cttgtacaca ccgtcttgta tggctgaata taagataaat ggagatggag
      961 aggctagaga agcaaccttg aaagctgcag ataagttgat tgaacgctat caagaaaaag
     1021 gtggttttat tcaagcttgg ggagacttgg gcaagaaaga gcattaccgt ttgattatcg
     1081 actgcttgct caatatccaa ctcttattct ttgcttatca agaaacaggc gatcaaaaat
     1141 actacgatat tgcagaaagt catttctatg cttcagctaa taatgtaatc cgtgatgacg
     1201 cttcgtcctt ccacaccttc tattttgatc ctgagacagg tcaacccttt aaaggtgtaa
     1261 cgagacaagg gtatagtgat gattcatgct gggcacgtgg tcaatcatgg ggagtctatg
     1321 gtattccttt gacttatcgt cacttaaaag acgagtcctg ctttgacttg tttaagggtg
     1381 tgaccaatta tttcttgaat cgtctgccaa aagatcatgt gtcctattgg gatttgattt
     1441 ttaatgatgg tagtgatcaa tcacgagatt cttcagcaac agctatcgcc gtctgtggga
     1501 ttcatgaaat gctaaaacat ctcccagagg tggatgctga caaagatatt tataaacatg
     1561 ctatgcatgc catgcttcgt tccttgatcg aacattatgc aaatgatcaa tttacccctg
     1621 gtgggacaag tctcctccac ggtgtgtact catggcattc aggtaaagga gtggatgaag
     1681 gcaatatctg gggtgactac tattacctag aagcccttat ccgtttctac aaagactgga
     1741 acctatattg gtaggaggag aaatatgaca atgccaaata ttattatgac ccgtatcgat
     1801 gaacggttga ttcatggaca aggacaactt tgggtaaaat acctaggttg taatacggtc
     1861 attgttgcca atgacgaagt aagcacggac aagatgcaac aaactctgat gaaaacagtt
     1921 gtgccagact cagttgccat gcgtttcttc cctttgcaaa aggtgattga tatcattcac
     1981 aaggctaatc ctgctcaaac gatctttatc gttgtaaagg atgtgaagga cgctttaacc
     2041 ttggtagaag gtggtgtccc tatcaaagaa atcaatattg ggaacattca caatgctact
     2101 ggtaaagagc aagtgacacg ctccatcttc ctgggtgaag aggacaaggc agccctcaag
     2161 gaattaagcc aaactcatca agtaacattt aatacgaaaa caactccaac aggaaatgat
     2221 ggagctgttc aagtcaacat tatggactat atttaaaaga ggagatcgtt atgtcgatta
     2281 atgtatttca agcgatttta attggattat ggacagcttt ctgttttagt ggaatgctat
     2341 taggaattta caccaataga tgtattgttc tgtcatttgg tgtcggaatt attctaggtg
     2401 atctgcctac tgctcttgca atgggagcta ttggtgaatt ggcttatatg ggattcggtg
     2461 ttggtgctgg aggtactgtt ccaccaaacc caatcggacc tggtatcttt ggtaccttga
     2521 tggctatcac tagtgctggt aaagtcagtc cagaagcggc tcttgccctc tctactccga
     2581 ttgctgtggc gattcaattc ttacaaactt tcgcctacac tgtacgtgct ggtgcgcctg
     2641 aaacggcgat gaagcacttg aaaaaccata atttgaagaa attcaagttc actctaaatg
     2701 caacaatttg gttgtttgcc tttattggat ttaccttggg ttgcttgggt gccctttcaa
     2761 tggatacctt gttgaaactc gtagactaca ttccaccggt attgcttaca ggtttgacag
     2821 ttgctggtaa aatgctccca gctatcggtt ttgcgatgat cttgtcagtg atggctaaga
     2881 aagagttgat tccctttgtc ttgttgggat atgtttgtgc agcttatcta aacatcccaa
     2941 caattggtat tgcaattgta ggtactatct ttgctttgat tgaattttat aacaagccaa
     3001 aaacagcgga tcatgtggta gaggaggaag cacacgatga ctggatctaa caaattaaca
     3061 aaacgtgatt atcttaaaac gtctttgcgg gcattctttt gtcaaaatgg atttaactat
     3121 agtaactatc aagggttggg atatgccaat gtgatgtatc ctgctttgaa aaaacactat
     3181 ggagagaatc aggaaggttt ctaccaagcc ttggaagaaa actgtgaatt ctataatacc
     3241 aacccacact tcctgccttt tattaccagc ttgcatcttg taatgttgga aaatggccgt
     3301 ccggcaaaag aaacacgtag catcaagatg gccttgatgg gaccattagc aggtattggg
     3361 gattctctct ctcaattctg tttagctcca ttgttctcaa ccatcgcagc ttcgtttgct
     3421 caagaaggct tggttgtcgg tccaatcttg ttcttccttg cgatgaatac gattttaaca
     3481 gcgattaaat tgtcaactgg tctgtatgga tacaaactag gaacgactgt gattgataaa
     3541 ctaagcgagc agatggcaac gatttctcgt attgccaata ttattggtgt aaccgtaatt
     3601 gctggtttgg cagcgacatc tgttaagatt atggtaccga tcacctttgc tgcaggggaa
     3661 gttaaagcag acgctaaaca aagtatcgta agtattcagg gaatgcttga taaggttgct
     3721 ccagctcttc taccagccct atttacactt ttagtttact acttgatcaa agaaaagaaa
     3781 tggacaacat ataaactcgt tattttaaca gttatcatcg gaattatcgg aagctggctt
     3841 aagattatag cttaatggat acaacattgt tttatggaat agtgattgtc ttggcagtga
     3901 gtcccctttt actgtcaagc tttcattcta ttcgtcagca aaagttgctt cgcaaacaga
     3961 tggagcaacg acaagagtat ttagcttctt taacatctgg tgatgaagtg ttgttattgt
     4021 ctggaattca tggaaaaatc atttctatcc aagatgactt ggtctccttg cagattgcaa
     4081 aaggtgtggt catctatgta gaaaaggaaa gtgtaatggg aaagacaaaa gaactgcttt
     4141 ttaagtagtt ctttttctgg taatagataa tgaaagaaga aagaagacaa ttttttgaaa
     4201 gaatcgatgg aaaccaatgt cgtgattata tcttgtccca ctgttcaaaa gactatgaga
     4261 aagtcaagag ttcccttgaa cgcttgatgg acaatcgttt tatgtttgat agtccttggg
     4321 atatggagcc ttgttcaaaa atccatcaaa tccagccgat ggtatgggat caagtatttg
     4381 aagatgatcc agaatggtct tatatgctca atcgacaaga atatctcttg cagtttatga
     4441 tagggtatgt ggtagaagga gataaggact atattcaaaa gtgcaagttc tttctatttg
     4501 attggattga gcaggtgaga gaattttctc ctcaatcctt gatgactaga accttggata
     4561 ccggtattcg ttcctttact tggttgaaac tactcttgct cctcttgaaa tttgacttgc
     4621 tagaggagaa agaactagag aaaattttgg tcagtctaga aaagcagatt gactttatga
     4681 aaagccacta tcgcgccaag tacaccctta gtaactgggg aattttacaa acaattccga
     4741 tgcttgctat ctatcatttc tttgcagata agatggacct agaagaagct taccattttg
     4801 cttcagagga gttgaaacag caaattgaga cacagatttt aggagatgga agccagtttg
     4861 aacagtcgat tctctatcat gtagaggttt ataaagcctt gctggatttg tgtctcttgc
     4921 ttccagactt gcaagatagt taccaagagt tgctggaaaa gatggcgacc tatattcaaa
     4981 tgatgacagg cttagatgga cgaactttgg cttttggtga tagcgattct acagaaacga
     5041 cagaaatgtt gagcctgtct gctgtggttt tgaacaagga agaccttctt aacggtctgg
     5101 atgttaaagt cgatttgctt agcctcttgt tcctggggcg agaaaaggtc aagcgactgc
     5161 aggaatttga aaagcgagct tggcagccta agttcatgag ctttgaagac tctggacatg
     5221 tctgcattaa ggatgaacat cgttatctat ttttcaaaaa tggtccgcta ggaagtgccc
     5281 atagccatag tgacgagaat agtttttgct tacagtatca aggccaacct attttcatag
     5341 atgctgggcg ttattcttat cgggagatat atgaacgtta tctcttaaag agtgcttgga
     5401 gtcattcgac ctgcattgta gatgggaaag ctccggaaag aatcacggga tcctgggaat
     5461 atgaatacta tcctcactcc ctgttttgtc accataaaga aagggaggga atgcattata
     5521 ttgaaggggc ttattggtca gcagaacctg atttgcctta tcttcacagg agaaaaatcc
     5581 tcatgttggt agaggatgtc tggctcttgg tagatgacat caggtgtcaa ggtcagcatg
     5641 aggcgttgac tcagtttatc cttgacaagg atgtgaccta tcaagatggg aaaatcaatc
     5701 agttgagact atggagtgaa gttgattttg atttggaaga taccatcatt tctcctaaat
     5761 acaatgagct tgaaagaagt agcaaactca ccaagcgcca attctttgag aatcagatgc
     5821 tggattatac catcattgcg catgagagtt ttgaaatcat ccgtcattct gtctaccaga
     5881 cagatgatcg tgaagtggaa aatgctctgg cttttgaagt gaaaaatgac gaaacagaca
     5941 agctgattct gttattaagc gaggatattt gtgtaggtga aaaattgtgc ctcgttgacg
     6001 gaacaaaaat gcgtggaaaa tgtctagtat atgataaaat aaatgagaga atgattcgct
     6061 tgcagtgcta gaaataggca ttttgaatag tgaatatgtt ataataagta ttagtaggag
     6121 gtgttttaga ttggagaaga aactgaccat aaaagacatt gcggaaatgg ctcagacctc
     6181 gaaaacaacc gtgtcatttt acctaaacgg gaaatatgaa aaaatgtccc aagagacacg
     6241 tgaaaagatt gaaaaagtta ttcatgaaac aaattacaaa ccgagcattg ttgcgcgtag
     6301 cttaaactcc aaacgaacaa aattaatcgg tgttttgatt ggtgatatta ccaacagttt
     6361 ctcaaaccaa attgttaagg gaattgagga catcgccagc cagaatggct accaggtaat
     6421 gataggaaat agtaattaca gccaagagag tgaggaccgg tatattgaaa gcatgcttct
     6481 cttgggagta gacggcttta ttattcagcc gacctctaat ttccgaaaat attctcgtat
     6541 catcgatgag aaaaagaaga aaatggtctt ttttgatagt cagctctatg aacaccggac
     6601 tagctgggtt aaaaccaata actatgatgc cgtttatgac atgacccagt cctgtatcga
     6661 aaaaggttat gaacattttc tcttgattac agcggatacg agtcgtttga gtactcggat
     6721 tgagcgggca agtggttttg tggatgcttt aacagatgct aatatgcgtc acgccagtct
     6781 aaccattgaa gataagcata cgaatttgga acaaattaag gaatttttac aaaaagaaat
     6841 cgatcccgat gaaaaaactc tggtatttat ccctaactgt tgggccctac ctctagtctt
     6901 taccgttatc aaagagttga attataactt gccacaagtt gggttgattg gttttgacaa
     6961 tacggagtgg acttgctttt cttctccaag tgtttcgacg ctggttcagc cctcctttga
     7021 ggaaggacaa caggctacaa agattttgat tgatcagatt gaaggccgca atcaagaaga
     7081 aaggcaacaa gtcttggatt gtagtgtgaa ttggaaagag tcgactttct aaaatgaagg
     7141 aaaatgactt gcaatctctg ttaagaaata aaataatccc acctagaaca agctaggtgg
     7201 gattatttgc ctatgaaatg agaaattatg ggagcaagct cctaaatcaa ctgtttttga
     7261 tctacttctt taactacttg ataaaagtta tagaagtagg ccaaacttga aatgatggtt
     7321 acgactagga atattgaaaa tttccattgg acagggttgg ttaaaagttg tggaaaggat
     7381 atgaggagaa agaagagggc tgcgttgagg acaggtatcc gttttgattg tattttctca
     7441 agtcctttat tgagcgcagg aagaaagagg agtaggagta gtaaaactgt atgagaaaca
     7501 gctcctgaaa taagggcaaa gaaaaggaaa atactgataa aaacatgaat gatcagtagt
     7561 ctagctagtg atttcataag gtacctccta atcctggtct tttttagctc ttgcaatacg
     7621 aagtgagtcg acaatatgta tcatcactcc gaaaaagaaa gctcccagta tagttttaaa
     7681 aatatgtttt gtatttagaa gagaactgat aaaatttgga ttttcacttg ttagggtatc
     7741 aatgagtgga attataaaaa atatcactgt tccataaatc gaacctgctt tcagaccagg
     7801 ataacgtaac tgtttctttt cttttttcat gagtttgctc cttttttaaa aatcttcctc
     7861 ccaaaagaga ctgttgaggt cagtttggag gctgcgggcg agattgagac agagttccaa
     7921 ggttggattg tacttgtcgt tttcaatcat attgatagtc tgtctcgaga caccgatatc
     7981 cttggcgagt tcgagctggg aaatacccaa ttccttgcga aattctttca cacgattcat
     8041 ctggtctcct ttctgattta tgtcgtatat atttgactat attatagtct tttaaacata
     8101 aagtgtcaag tatttttgac atattttttg aagaaatagt agtctccttg tcctatttgt
     8161 ctgacaagtg caagctggtc ggatttgtgg taaaatagat aagatatgac aaaagaattt
     8221 catcatgtaa cggtcttact ccacgaaacg attgatatgc ttgacgtaaa gcctgaaggt
     8281 atctacgttg atgcgacttt gggcggagca ggacatagcg agtatttatt aagtaaatta
     8341 agtgaaaaag gccatctcta tgcctttgac caggatcaga atgccattga caatgcgcaa
     8401 aaacgcttgg caccttacat tgagaaggga atggtgacct ttatcaagga taacttccgt
     8461 catttacagg cacgtttgcg cgaagctggt gttcaggaaa ttgatggaat ttgttatgac
     8521 ttgggagtgt ctagtcctca attggaccag cgtgagcgtg gtttttctta taaaaaggat
     8581 gcgccactgg acatgcggat gaatcaggat gctagtctga cagcctatga agtggttaat
     8641 cattatgact atcatgattt ggttcgtatt ttcttcaaat acggtgagga taaattctct
     8701 aaacagattg cgcgtaagat tgagcaagcg cgtgaagtga agccgattga gacaacgact
     8761 gagttagcag agattatcaa gttggtcaaa cctgccaagg aactcaagaa gaagggtcat
     8821 cctgctaagc agattttcca ggctattcga attgaagtca atgatgaact gggagcggca
     8881 gatgagtcca tccagcaggc tatggatatg ttggctctgg atggtagaat ttcagtgatt
     8941 acctttcatt ccttagaaga ccgcttgacc aagcaattgt tcaaggaagc ttcaacagtt
     9001 gaagttccaa aaggcttgcc tttcatccca gatgatctca agcccaagat ggaattggtg
     9061 tcccgtaagc caatcttgcc aagtgcggaa gagttagaag ccaataaccg ctcgcactca
     9121 gccaagttgc gcgtggtcag aaaaattcac aagtaagagg gaaaaagatg gcagaaaaaa
     9181 tggaaaaaac aggtcaaata ctacagatgc aacttaaacg gttttcgcgt gtggaaaaag
     9241 ctttttactt ttccattgct gtaaccactc ttattgtagc cattagtatt atttttatgc
     9301 agaccaagct cttgcaagtg cagaatgatt tgacaaaaat caatgcgcag atagaggaaa
     9361 agaaaaccga attggacgat gccaagcaag aggtcaatga actattacgt gcagaacgtt
     9421 tgaaagaaat tgccaattca cacgatttgc aattaaacaa tgaaaatatt agaatagcgg
     9481 agtaagatat gaagtggaca aaaagagtaa tccgttatgc gaccaaaaat cggaaatcgc
     9541 cggctgaaaa cagacgcaga gttggaaaaa gtctgagttt attatctgtc tttgtttttg
     9601 ccattttttt agtcaatttt gcggtcatta ttgggacagg cactcgcttt ggaacagatt
     9661 tagcgaagga agctaagaag gttcatcaaa ccacccgtac agttcctgcc aaacgtggga
     9721 ctatttatga ccgaaatgga gtcccgattg ctgaggatgc aacctcttat aatgtctatg
     9781 cggtcattga tgagaactat aagtcagcaa cgggtaagat tctttacgta gaaaaaacac
     9841 aatttaacaa ggttgcagag gtctttcata agtatctgga catggaagaa tcctatgtaa
     9901 gagagcaact ctcgcaacct aatctcaagc aagtttcctt tggagcaaag ggaaatggga
     9961 ttacctatgc caatatgatg tctatcaaaa aagaattgga agctgcagag gtcaagggga
    10021 ttgattttac aaccagtccc aatcgtagtt acccaaacgg acaatttgct tctagtttta
    10081 tcggcctagc tcagctccat gaaaatgaag atggaagcaa gagcttgctg ggaacctctg
    10141 gaatggagag ttccttgaac agtattcttg cagggacaga cggcattatt acctatgaaa
    10201 aggatcgtct gggtaatatt gtacccggaa cagaacaagt ttcccaacga acgatggacg
    10261 gtaaggatgt ttatacaacc atttccagcc ccctccagtc ctttatggaa acccagatgg
    10321 atgcttttca agagaaggta aaaggaaagt acatgacagc gactttggtc agtgctaaaa
    10381 caggggaaat tctggcaaca acgcaacgac cgacctttga tgcagataca aaagaaggca
    10441 ttacagagga ctttgtttgg cgtgatatcc tttaccaaag taactatgag ccaggttcca
    10501 ctatgaaagt gatgatgttg gctgctgcta ttgataataa tacctttcca ggaggagaag
    10561 tctttaatag tagtgagtta aaaattgcag atgccacgat tcgagattgg gacgttaatg
    10621 aaggattgac tggtggcaga atgatgactt tttctcaagg ttttgcacac tcaagtaacg
    10681 ttgggatgac cctccttgag caaaagatgg gagatgctac ctggcttgat tatcttaatc
    10741 gttttaaatt tggtgttccg acccgtttcg gtttgacgga tgagtatgct ggtcagcttc
    10801 ctgcggataa tattgtcaac attgcgcaaa gctcatttgg acaagggatt tcagtgaccc
    10861 agacgcaaat gattcgtgcc tttacagcta ttgctaatga cggtgtcatg ctggagccta
    10921 aatttattag tgccatttat gatccaaatg atcaaactgc tcggaaatct caaaaagaaa
    10981 ttgtgggaaa tcctgtttct aaagatgcag ctagtctaac tcggactaac atggttttgg
    11041 tagggacgga tccggtttat ggaaccatgt ataaccacag cacaggcaag ccaactgtaa
    11101 ctgttcctgg gcaaaatgta gccctcaagt ctggtacggc tcagattgct gacgagaaaa
    11161 atggtggtta tctagtcggg gtaaccgact atattttctc ggctgtatcg atgagtccgg
    11221 ctgaaaatcc tgattttatc ttgtatgtga cggtccaaca acctgaacat tattcaggta
    11281 ttcagttggg agaatttgcc aatcctatct tggagcgggc ttcagctatg aaagactctc
    11341 tcaatcttca aacaacagct aaagctttgg agcaagtaag tcaacaaagt ccttatccta
    11401 tgcctagtgt caaggatatt tcacctggtg atttagcaga agaattgcgt cgcaatcttg
    11461 tacaacccat cgttgtggga acaggaacga agattaaaaa cagttctgct gaagaaggga
    11521 agaatcttgc cccgaaccag caagtcctta tcttatctga taaagtagag gaagttccgg
    11581 atatgtatgg ttggacaaag gagactgctg agacccttgc taagtggctc aatatagaac
    11641 ttgaatttca aggttcgggc tctactgtgc agaagcaaga tgttcgtgct aacacagcta
    11701 tcaaggacat taaaaaaatt acattaactt taggagacta atatgtttat ttccctcagt
    11761 gctggaattg tgacattttt actaacttta gtaggaattc cggcctttat ccaattttat
    11821 agaaaggcgc aaattacagg ccagcagatg catgaggatg tcaaacagca tcaggcaaaa
    11881 gctgggactc ctacaatggg aggtttggtt ttcttgatta cttctgtttt ggttgctttc
    11941 tttttcgccc tatttagtag ccaattcagc aataatgtgg gaatgatttt gttcatcttg
    12001 gtcttgtatg gcttggtcgg atttttagat gactttctca aggtctttcg taaaatcaat
    12061 gaggggctta atcctaagca aaaattagct cttcagcttc taggtggagt tatcttctat
    12121 cttttctatg agcgcggtgg cgatatgctt tctgtctttg gttatcaagt gcatctaggg
    12181 attttctata ttgtcttcgc tcttttctgg ctagtcggtt tttcaaacgc agtaaacttg
    12241 acagacggtg ttgacggttt agctagtatt tccgttgtga ttagtttgtc tgcctatgga
    12301 gttattgcct atgtgcaagg tcagatggat attcttctag tgattcttgc catgattggt
    12361 ggtttgctcg gtttcttcat ctttaaccat aagcctgcca aggtctttat gggtgatgtg
    12421 ggaagtttgg ccctaggtgg gatgctggca gctatctcta tggctctcca ccaagaatgg
    12481 actctcttga ttatcggaat tgtatatgtt tttgaaacaa cttctgttat gatgcaagtc
    12541 agttatttca aactgacagg tggtaaacgt attttccgta tgacgcctgt acatcaccat
    12601 tttgagcttg ggggattgtc tggtaaagga aatccttgga gcgagtggaa ggttgacttc
    12661 ttcttttggg gagtgggact tctagcaagt ctcctgaccc tcgcaatttt atatttgatg
    12721 taagaatggc accctgatgt ttcagggtgt ttttgtgttt aaatacacaa tgaaaatcaa
    12781 agaacaaact agaaagctaa ctttaggctg ctcaaaatat agtagattga aactagaata
    12841 gtacacatct acttctaaaa tattgttaga aatcgatttg actgtcctga tcgatttgtc
    12901 ctgttcttat ttcattttaa tatacagttt tgaggttgta gataaggaga agctgatgtg
    12961 gtttgaagag gttttcgaag agtgtaaaaa aatattggtc agttaaagtc aaaactcttg
    13021 accttttctg actaatgtag tatattatga acgtaaggta aatgaaagcc ttactagaac
    13081 acttatttaa agtaaaatag aaagaggtgg gtctatgttt catctagaaa tcttcagaag
    13141 taaagatagt ctactcctgc ttgaaaaaga aaaaccagaa atagtacgta gggtagcgat
    13201 ctagctagtc taaatttttt aaaacaatgg tcaaagatag tcaatatcag taatcataac
    13261 taagtaaaca aaaagaggta aagaatatga ataacaactt taataatttt aataacatgg
    13321 atgatttatt taaccaattg atgggtggta tgcgaggata cagttctgaa aatcgccgtt
    13381 acttaattaa tggacgcgaa gtcacacctg aggaatttgc tcactatcgt acgactggtc
    13441 aattaccagg aaatgcagaa actgatgtgc aaatgccaca acaggcatca ggtatgaaac
    13501 aagacggtgt ccttgcaaaa ctaggtcgaa acttgacagc agaagcgcgt gagggcaagt
    13561 tggatcctgt tatcggacga aacaaggaaa ttcaagaaac atctgaaatc ctctcacgcc
    13621 gcaccaagaa caatcctgtt ttggtcggag atgcaggtgt tggtaagaca gcagttgtcg
    13681 aaggtctagc gcaagccatt gtgaacggag atgttcctgc tgctatcaag aacaaggaaa
    13741 ttatttctat tgatatctca ggtcttgagg ctggtactca ataccgtggt agctttgaag
    13801 aaaatgtcca aaacttagtc aatgaagtga aagaagcagg gaatattatc ctcttctttg
    13861 atgaaattca ccaaattctt ggtgctggta gcactggtgg agacagtggt tctaaagggc
    13921 ttgcggatat tctcaagcca gctctctctc gtggagaatt gacagtgatt ggggcaacaa
    13981 ctcaagacga ataccgtaac accatcttga agaatgctgc tcttgctcgt cgtttcaacg
    14041 aagtgaaggt caatgctcct tcagcagaga atacttttaa aattcttcaa ggcattcgtg
    14101 acctctatca acaacaccac aatgtcatct tgccagacga agtcttgaaa gcagcggtgg
    14161 attattctgt tcaatacatt cctcaacgta gcttgccaga taaggctatt gaccttgtcg
    14221 atgtaacggc tgctcacttg gcggctcaac atccagtaac agatgtgcat gctgttgaac
    14281 gagaaatcga aacggaaaaa gacaagcaag aaaaagcagt tgaagcagaa gattttgaag
    14341 cagctctaaa ctataaaaca cgcattgcag aattggaaaa gaaaatcgaa aaccacacag
    14401 aagatatgaa agtgactgca agtgtcaacg atgtggctga atctgtggaa cgaatgacag
    14461 gtatcccagt atcgcaaatg ggagcttcag atatcgaacg tttgaaagat atggctcatc
    14521 gcttgcaaga caaggtgatt ggtcaagata aggccgtaga agctgtagct cgtgctatcc
    14581 gtcgtaaccg tgctggtttt gatgaaggaa atcgcccaat cggcagcttc ctctttgtag
    14641 ggtctactgg ggttggtaag acggagcttg ctaagcaatt ggcactcgat atgtttggaa
    14701 cccaggatgc gattatccgt ttggatatgt ctgaatacag tgaccgcaca gctgtttcta
    14761 agctaattgg tacaacagca ggctatgtgg gttatgatga caatagcaat accttaacag
    14821 aacgtgttcg tcgcaatcca tactctatca ttctcttgga tgaaattgaa aaggctgatc
    14881 ctcaagttat tacccttctc ctccaagttc tagatgatgg tcgtttgaca gatggtcaag
    14941 gaaatacagt aaacttcaag aacactgtca ttattgcgac ctcaaatgct ggatttggct
    15001 atgaagccaa cttgacagaa gatgcggata aaccagaatt gatggaccgt ttgaaaccct
    15061 tcttccgtcc agaattcctc aaccgcttta atgcagtcat cgagttttca cacttgacta
    15121 aggaagacct ttctaagatt gtagatttga tgttggctga agttaaccaa accttggcta
    15181 agaaagacat tgacttggta gtcagtcaag cggctaaaga ttatatcaca gaagaaggtt
    15241 acgacgaagt catgggggtt cgtcctctcc gtcgcgtggt tgaacaagaa attcgtgata
    15301 aggtgacaga cttccacttg gatcatttag atgctaaaca tctggaagca gatatggaag
    15361 atggcgtttt ggttattcgt gagaaagcct aagacagaat tttgagaata aaaaagaagg
    15421 agccagctga aaaaaactgg ttccttttga ggtacgacgg gcatgtcgtg tagtagagat
    15481 gtactattct agtttcaatc tactatagta gctcagaaat cggtacttaa acgtgctata
    15541 tcaaaaccag tccttgaaaa acgtggactg gtttcgtgtt tggattatta ctttgaacga
    15601 catgcgttaa aagttagttg aaccgccgta tgccgaacgg tacgtacggt ggtgtgagag
    15661 gggctagaga ttatccccta ctcgatttta aatcacatga cgttcaaagg catcatctga
    15721 aatcccttgt tccaagatga gttttgccca ttctttagca gagaagaggc tgtggtcctt
    15781 gtagtttccg caagattcga tggttgtccc tgggacatct tcccaagtag tagtttcagc
    15841 gatttccttg agcgaatcct tgataacagc tgcgatttta gcactggtgt gacgtcccca
    15901 cataatcatg tggaagcctg tgcggcaacc aaatggcgaa cagtcaatca tgccgtcaat
    15961 gcgggtacgg atgagtttgg ctaagaggtg ctcgatagtg tgaaggccgg cggtagggat
    16021 agagtcttcg tttggttgca ccaagcgaat atcataattg gagatgatgt ctccttttgg
    16081 tcctgtttct tccccaatca agcgaacata gggtgctttg acaatggtgt ggtcaagttc
    16141 aaaactttcg acaataactt cttttgacat ggtaaatcct ttcagttttc ttctctcatt
    16201 atatcataaa ggttgctcct gagacagaga gaaaacctct ccgaggctgg agaggttgaa
    16261 atctttactt acgatataag cggtcgtatt ggtagtatgg gtcaaaggtt acgttgatac
    16321 ccagtttacg aaggacattc ttgtcttcat cagtcaagat gatggttgag tgggcttcgc
    16381 ttcctttgag gttgccgagt tcttccatag cgcgggtagc atcaggattt tctgtagctg
    16441 tgatagcaag tgcaatcagg atttcatttg aatgaaggcg tggattgcgg ctaccgagat
    16501 gatcgatttt aagaccttgg attggcttaa caacttccgg ctcgattaat tttacttctt
    16561 tagcgatgtc agctgatttt ttgatggcgt tgatcaaggc agcggctgta gggccaaaga
    16621 gttctgagtt cttaccagtg acgatttccc catttggcaa ttcaaaggct agggctggtc
    16681 caccagtttc ttctgctttt tggcgcgcaa cgacagcaac cttacggtct gcaggtgtga
    16741 taccgaggtc gttcatgagc aactcaattt tcttgacggc agcttcgcca actttttcag
    16801 ctttgaagtc aagaactgtt tgatagtaac ggcggatgat ttcttgttta gaagcttcga
    16861 cagcggcctc gtcatctgta atagcgaaac caaccatgtt gacacccata tctgtcggtg
    16921 aagcgtatgg tgattttccg agaatacgtt ccaacatgcg tttgagcact gggaagattt
    16981 cgatatcacg gttgtagttg acagtggttt ctccataggt ttggagatgg aaggggtcaa
    17041 tcatgttgac atcatcaagg tcagctgtgg cagcttcata agccaagtta actggatgat
    17101 gaaggggaag attccaaaca gggaaggttt caaatttagc gtagccagat ttgatgccat
    17161 tgatttggtc gtggtacata ttggacatac acgttgccaa ttttccagaa ccaggtccag
    17221 gagcggttac gacaatcaag ttgcgactgg ttttgatgta gtcgtttttg cccatgcctt
    17281 ctggggaaat gatgtgatcc atatccgtcg gatatccttt gattggataa tgaagatatg
    17341 aatcaattcc gtttttctca agttggttgc ggaaggcatc tgcagcgggt tggccagcgt
    17401 attgtgtaat gacaacggaa ccaacaaaaa tccccaattc attgaatttg tcaatcaaac
    17461 gaagaacttc ttggtcataa gaaatgccta agtcgccacg tgctttggaa tgttcaatgt
    17521 tgctagcatt aatggcaatc acaacctcaa cctgctcttt caattcttgc aagagcttga
    17581 ttttgttgtc aggttcataa ccaggaagga cacgagcagc gtggaaatct tctaacattt
    17641 taccgccaaa ctctaagtag agcttgccgt caaattggtt aatacgctcc aaaatatggt
    17701 cgcgttgtaa attcaaatat tgttcagaac taaaagcttg ttttttcatt tttttacctc
    17761 tggactctat tataataaaa aattggaagt taggaaacta cggagctaaa aaagaaatta
    17821 aaaagattaa gcaaacgctt gcacaaaatt ttaaaaagtg ctatcataga ctatagatta
    17881 ttaaaataat gaggtaaaca gatgcaagaa aaatggtggc ataatgccgt agtctatcaa
    17941 gtctatccaa agagttttat ggatagtaat ggagatggag ttggtgattt gccaggtatt
    18001 accagtaagt tggactatct agccaagcta ggaatcacag caatttggct ttctcccgtt
    18061 tatgacagcc ctatggatga taatggttac gatattgctg attatcaagc gattgcaacg
    18121 atttttggaa ccatggagga tatggatcaa ctgattgcag aagctaagaa gcgtgatatc
    18181 cgtatcatca tggacttggt ggtcaatcat acctcggatg agcatgcctg gtttgtagag
    18241 gcctgtgaaa atcctaatag ccctgagcga gactactata tctggcgcga tgaacccaat
    18301 gacctagatt ctatctttag tgggtctgct tgggaatacg atgaaaagtc aggtcaatac
    18361 tatctccact ttttcagcaa gaaacagccg gatctcaact gggaaaatga aaaacttcgc
    18421 cagaaaattt atgagatgat gaacttctgg attgataaag gtattggtgg tttccgtatg
    18481 gatgttattg acatgattgg caaaattcct gacgagaagg tagtcaataa tggtcctatg
    18541 ctccatccct atctcaagga aatgaatcag gcgacctttg gagataagga tctcttgaca
    18601 gtaggggaga cttggggagc aacgccagag attgccaagc tctactctga tccaaagggg
    18661 caagaattgt ctatggtctt ccagtttgaa catatcggtc ttcagtatca ggaaggtcag
    18721 cctaaatggc actatcaaaa agagctgaat atcgctaagt taaaagaaat cttcaacaaa
    18781 tggcagacag agttaggagt tgaggacggc tggaattccc tcttctggaa caaccatgac
    18841 ctccctcgta ttgtctcaat atggggaaat gaccaagaat accgcgaaaa atctgccaaa
    18901 gcctttgcaa tcttgcttca tcttatgaga ggaactcctt atatctacca aggtgaggag
    18961 attgggatga ccaactatcc gtttgaaaca ctagatcaag tagaagatat tgaatctctc
    19021 aactatgcgc gtgaggctct tgaaaaaggt gttccgattg aagaaatcat ggacagtatc
    19081 cgtgttattg gacgtgacaa tgcccgtacc cctatgcaat gggacgagag caaaaacgct
    19141 ggtttctcaa caggtcaacc ttggttggcg gttaatccaa attacgagat gatcaacgtc
    19201 caagaagcgc tggcaaatcc agattctatt ttctatacct atcagaaact ggtccaaatt
    19261 cgcaaggaga atagctggct agttcgagct gactttgaat tgcttgatac ggctgataag
    19321 gtctttgctt atatacgtaa ggatggcgac cgtcgcttcc tagttgtggc taacttgtcc
    19381 aatgaagagc aagacttgac agtagaagga aaagtcaaat ctgtcttgat tgaaaacacc
    19441 ctagctcaag aagtctttga aaaacaaatc ttagttccat gggatgcttt ctgtgtggaa
    19501 atgactgact agaatgagca aacctcaagt ttttgaagct tgaggttttt tactatagtg
    19561 gattgaaact agaatagtgc acctctgctt ctaaaacatt gttagaaatc gatttgactg
    19621 tcccgatcga tttgtcctat tcttatttca ttttgctata cttgtagttg aggagaatta
    19681 agctcctcac tagtaaactc ttcataatcc tttttatttt tatgaagata ttgtttgaaa
    19741 gatgtgagtt tccacggatg ggtttgtgga gggatatact tgcgtctttc tttttttgtt
    19801 ctggttcttg ttcaaagttt ttcgaataga gttcatgatt tagtagctcc tttgtgtgat
    19861 agattttgtc agcgatattg aggtagatgt ctccgtcaaa tgcttttata actaatgctt
    19921 tcgtctttct gatgaaatag acttcttttc cttgttcggt agggatatag taacgatttt
    19981 ggaatcggat atggtgtcca ctatcgacga ctctctccgc cagtctagcc agaatgagat
    20041 tcctttcaga ggggttagga acctcctcga agacagagag ttttgtcttg tttccaaact
    20101 gttcattaaa ggtttgaatg taggaaagca ggaaggtatt ggcttcttcc aaggtatgaa
    20161 tattgttttg ttccagttcg ataggcaggc gagattgtag tgtctgattg agtctttcga
    20221 ctctcccttt agcttgaggg atagaggttg tctcaaggag aatccctagt tggtgacagg
    20281 cgtatccaaa ctgtgtatgg gtatcgtcct ccattttctt agagttggag gcttgatagg
    20341 taaagaccgt tctcttatct gttttaattt gaaggggaat gccgtgattg gctaagattt
    20401 gttcgaggac atgatagtaa gcattcaagt tctcttgttt atcaaaataa gcgcctagga
    20461 tattgccaga agcatcatca atggctaagt gtaagttaga tgtttgggct ccaaactagg
    20521 catgagggct ggcatccatt tgaatgagtt caccagcaaa tttctttctg ggtctactag
    20581 gatgtacttt tttagggtct tcaaggaagt tttcagccgt cggtaagatt ggattgtcta
    20641 ggggttgatt caggttcagt ttagcttgtt ttcttactct cttctttgtc tttctatggg
    20701 acttaggcga caggatattt ttcttataga gtatttttct aacagtagta tcagagagct
    20761 gaattccttc ttcttcagct agcaattcac agaaatgaag gacatttggt ttatatgttt
    20821 cataggagag gtattttttt aggagacgtt ctttgatttc atcagggatt gcatgttttg
    20881 gttttcgatt tctgtttccg tgtctgaagg cttcttttcc tttcagttga taggctagta
    20941 gcagacgatt gatttgtctt tcagaaagat tgagctcgac acaggctcgt ttctttgttt
    21001 tctttccttg ggctatagct tttatcacaa gatatttttt cgtttcattc atattcagtt
    21061 ggatcctttt catatgacta ttctaccaaa tgggacattt tcacgttcga tttactaaag
    21121 acattatcac attcgaatta cacaagatgc agatagtgaa aataaaggtg tagacattac
    21181 cgtaaaaaag tgatataatt gtatgatgtt caatgtatag gtgttaatca tgagtagacg
    21241 ttttaaaaaa tcaggttcac agaaagtgaa gcgaagtgtt aatatcgttt tgctgactat
    21301 ttatttattg ttagtttgtt ttttattgtt cttaatcttt aagtacaata tccttgcttt
    21361 tagatatctt aatctagtgg taactgcgtt agtcctacta gttgccttag tagggctact
    21421 cttgattatc tataaaaaag ctgaaaagtt tactattttt ctgttggtgt tctctatcct
    21481 tgtcagctct gtgtcgcttt ttgcagtaca gcagtttgtt ggactgacca atcgtttaaa
    21541 tgcgacttcg aattactcag aatattcaat cagtgtcgct gttttagcag atagtgagat
    21601 cgaaaatgtt acgcaactga cgagtgtgac agcaccgact gggactgata atgaaaatat
    21661 tcaaaaacta ctagctgata tcaagtcaag tcagaatacc gatttgacgg tcaaccagag
    21721 ttcgtcttac ttggcagctt acaagagttt gattgcaggg gagactaagg ccattgtcct
    21781 aaatagtgtc tttgaaaata tcatcgagtc agagtatcca gactacgcat cgaagataaa
    21841 aaagatttat accaagggat tcactaaaaa agtagaagct cctaagacgt ctaagaatca
    21901 gtctttcaat atctatgtta gtggaattga cacctatggt cctattagtt cggtgtcgcg
    21961 atcagatgtc aatatcctga tgactgtcaa tcgagatacc aagaaaatcc tcttgaccac
    22021 aacgccacgt gatgcctatg taccaatcgc agatggtgga aataatcaaa aagataaatt
    22081 aacccatgcg ggcatttatg gagttgattc gtccattcat accctagaga acctttatgg
    22141 tgtagatatt aattattatg ttcgtttgaa ttttacctca tttttgaaat tgattgactt
    22201 attgggaggg gtagatgttc ataatgatca agagttttca gctctacata ggaagttcct
    22261 tttcccagta gggaatgtcc atctagactc tgagcaggct ctaggttttg tacgtgaacg
    22321 ctactcacta gccgatggag accgtgaccg tggtcgcaac caacaaaagg tgattgtggc
    22381 tattcttcaa aaattaacgt caaccgaagc actgaaaaat tatagtacga tcattaatag
    22441 cttgcaagat tctatccaaa caaatatgcc acttgagact atgataaatt tggtcaatgc
    22501 tcagttagaa agtggtggaa cttacaaagt gaattctcaa gatttaaaag ggacagatcg
    22561 gatggatctt ccttcttatg caatgcctga cagtaacctc tatgtgatgg aaatagatga
    22621 tagtagttta gctgtagtta aagcagctat acaggatgtg atggagggta gatgaaatga
    22681 tagacatcca ttcgcatatc gtttttgatg tagatgacgg tcccaagtca atagaggaaa
    22741 gcaaggcact tttaagagaa gcttataatc aaggagttcg aatgattgtg tctacttcgc
    22801 atcgtcgaaa agggatgttt gaaactccgg aagagaagat agcagaaaac tttcttcagg
    22861 ttcgggaaat tgcaaaagaa gtagcagatg atttagtcat agcttatggc gcagagatat
    22921 actatactct ggatgctcta gaaaagctag aaaaaaaaga aattcctacc cttaatgata
    22981 gtcgttatgc tttgattgag tttagcatgc atacttccta tcgtgagatt catgcgggat
    23041 tgagcaatat tttgatgttg ggaatcacgc cagtaattgc tcatattgaa cgttatgatg
    23101 ccttagagaa taacgaaaaa cgtgttcgtg aactgattga tatggggtgc tatactcaga
    23161 taaatagtta tcatgtttta aaacctaagt tctttggtga aaaatataaa ttcatgaaaa
    23221 agagagctcg gtattttttg gaacgtgatt tagttcatgt agttgcaagt gacatgcaca
    23281 atttagacag tagacctcca tatatgcaac aggcatatga tatcattgct aagaaatatg
    23341 gagcgaaaaa agcgaaagaa ttttttgtag ataatcccag aaaaattata atggatcaat
    23401 taatttagga gaaaatatga aggaacaaaa cactttggaa atcgatgtat tgcaactatt
    23461 cagggcttta tggaaaagaa agttggtcat tttattagtg gcaattataa cttcttcagt
    23521 tgcttttgcc tacagtactt ttgttatcaa acctgagttt actagtacga ctcggattta
    23581 tgtagttaac cgtaatcagg aagagaagtc tggtttaacc aatcaagact tgcaggcagg
    23641 atcatacttg gttaaagact atcgtgaaat tatcctatcg caggatgttt tggaggaagt
    23701 tatttctgat ttgaaactag atttgacgcc aaaaggtttg gctaataaaa ttaaagtaac
    23761 agtaccagtt gatacccgta ttgtctctgt ctcagttaat gatcgagttc ctgaagaggc
    23821 aagccgtatc gctaactctt tgagagaagt agctgctcaa aaaattatca gtattactcg
    23881 tgtttctgat gtgacaacac tggaggaggc aagaccggcg atatcaccat cttcgccaaa
    23941 tattaaacgc aatacactaa ttggtttttt ggcagggggg attggaacta gtgttatagt
    24001 tcttcttctt gaacttttgg acactcatgt gaaacgtccg gaagatatcg aagatacact
    24061 gcagatgaca cttttgggag ttgtaccaaa cttgagtaag ttgaaatagg agagaggaat
    24121 gccgacatta gaaatagcac aaaaaaaact ggagttcatt aagaaggcag aagaatatta
    24181 caatgccttg tgtacaaata tacagttgag cggagataaa ctaaaagtaa tttccgttac
    24241 ttctgttaac cctggggaag gaaaaacaac tacttccgta aatatagcaa ggtcgtttgc
    24301 gcgtacaggc tataaaactc ttttgatcga tggcgatact cgaaattcag ttatatcagg
    24361 agtttttaaa tcgcgtgaaa aaattacagg gctaacagaa tttttatctg ggacagctga
    24421 tttatctcac ggtttatgtg atacaaatat tgaaaattta tttgtaattc aatcgggatc
    24481 tgtatcacca aaccctacag ccttgttaca aagtaaaaat tttaatgata tgattgaaac
    24541 attgcgtaaa tattttgatt atatcattgt tgatacagca cctattggaa ttgttattga
    24601 tgcggcaatt atcactcaaa agtgtgatgc gtccatcttg gtaacagcaa taggtgaggt
    24661 gaataaacgt gatgtccaaa aagcgaaaca acaattagaa caaacagaga aactgttcct
    24721 aggagttgtt ttaaataaat tggatatctc ggttgataag tatggagttt acggttccta
    24781 tggaaattat ggtaaaaaat aacttaggaa agattttatg gatgaaaaag gattgaaaat
    24841 ttttctagca gtaatacaga gtattattgt cattttattg gtttattttc ttagctttgt
    24901 tagagagaca gaacttgaac gttcttcgat ggttatacta taccttctcc acttttttgt
    24961 attctatttt agttcctatg gtaacaattt ttttaaaaga gggtacctag ttgagtttaa
    25021 tagtactata agatatattt ttttctttgc aatagctata agtgtattaa acttttttat
    25081 agcggaacgg tttagtatct ctagaagagg aatggtatac ttcttaactt tagaaggaat
    25141 atccttatac ttgttaaatt tc
//
LOCUS       DAVLVF010000023        22190 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVLVF010000023 DAVLVF010000000
VERSION     DAVLVF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.023"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            856..1077
                     /locus_tag="VYN66_000915"
     CDS             856..1077
                     /locus_tag="VYN66_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1494969.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            1594..1847
                     /locus_tag="VYN66_000916"
                     /pseudo
     CDS             1594..1847
                     /locus_tag="VYN66_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1975..3204
                     /locus_tag="VYN66_000917"
     CDS             1975..3204
                     /locus_tag="VYN66_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1494970.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFAGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            3224..5920
                     /gene="ppc"
                     /locus_tag="VYN66_000918"
     CDS             3224..5920
                     /gene="ppc"
                     /locus_tag="VYN66_000918"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV1494971.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    6003..6220
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6333..7337
                     /locus_tag="VYN66_000919"
     CDS             6333..7337
                     /locus_tag="VYN66_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1494972.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            7406..8272
                     /locus_tag="VYN66_000920"
     CDS             7406..8272
                     /locus_tag="VYN66_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1494973.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            8269..9027
                     /locus_tag="VYN66_000921"
     CDS             8269..9027
                     /locus_tag="VYN66_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1494974.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            9117..10877
                     /gene="dnaG"
                     /locus_tag="VYN66_000922"
     CDS             9117..10877
                     /gene="dnaG"
                     /locus_tag="VYN66_000922"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV1494975.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRAGI
                     ESAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10880..11989
                     /gene="rpoD"
                     /locus_tag="VYN66_000923"
     CDS             10880..11989
                     /gene="rpoD"
                     /locus_tag="VYN66_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV1494976.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            12003..12332
                     /locus_tag="VYN66_000924"
     CDS             12003..12332
                     /locus_tag="VYN66_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1494977.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            12447..13490
                     /gene="cpoA"
                     /locus_tag="VYN66_000925"
     CDS             12447..13490
                     /gene="cpoA"
                     /locus_tag="VYN66_000925"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV1494978.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            13506..14831
                     /locus_tag="VYN66_000926"
     CDS             13506..14831
                     /locus_tag="VYN66_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1494979.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14862..15038
                     /locus_tag="VYN66_000927"
     CDS             14862..15038
                     /locus_tag="VYN66_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1494980.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            15097..15231
                     /locus_tag="VYN66_000928"
     CDS             15097..15231
                     /locus_tag="VYN66_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV1494981.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            15295..16605
                     /gene="obgE"
                     /locus_tag="VYN66_000929"
     CDS             15295..16605
                     /gene="obgE"
                     /locus_tag="VYN66_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV1494982.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            16615..16773
                     /locus_tag="VYN66_000930"
     CDS             16615..16773
                     /locus_tag="VYN66_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1494983.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            17860..19119
                     /locus_tag="VYN66_000931"
     CDS             17860..19119
                     /locus_tag="VYN66_000931"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1494984.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            19135..19686
                     /locus_tag="VYN66_000932"
     CDS             19135..19686
                     /locus_tag="VYN66_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1494985.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            19679..20956
                     /locus_tag="VYN66_000933"
     CDS             19679..20956
                     /locus_tag="VYN66_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1494986.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFWVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            20972..21832
                     /locus_tag="VYN66_000934"
     CDS             20972..21832
                     /locus_tag="VYN66_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV1494987.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
BASE COUNT         6808 a         3794 c         4910 g         6678 t
ORIGIN      
        1 tattaaccaa tataatattt cccaatataa tgtcatttct tttattttta cctatagcta
       61 tctttatgca aatttgctgt ataagatttg attccatttt agcaaaggcc ttacctaata
      121 ttttgctgat acttattgtt ttaatcacgt ttcagagtga ttggaatata tttatacctg
      181 caacttatta ttatacagaa attcagtcaa ctacaaactt aggtattaaa ttattagttt
      241 gtatctggat tatgggattt gaatttttcc ttcttccaaa actaataaaa ttgaaagaac
      301 aaaatctagt ttaaatgttg ctcgaaaatt aaataattga atacgattaa caatgaaaca
      361 atacaacgtg agtcatgact tcaaagtaat tgattttatg attctggttt cttacgctta
      421 attaagcaag ttgtattcac tttatcaaat tgttattact ctaaaaagcg tctaacagct
      481 atagagtgtc tgtttgcttc tctttacttt tattttaaat aattcattaa ccttccgtaa
      541 gtgcccaata ataaggtgtg ttgaagaagt ctgagataaa atagttctca gtcacaaaaa
      601 agctatagat tcccattggt gaacagctcc ctgtcaagta gacagtaaaa taatataaaa
      661 tcttaagcga cctgattcct gaattccagt ggagtcaggt tgttttttaa ccgtctttct
      721 taatattgtc ttacctataa tagatatgct tatgagatat gtttaataaa ttaattttag
      781 ttcaaaatgt ccagttagga cactttgaat gatttcttag ttttatatat tatacttttc
      841 tcaggagaaa gctagatgta cagacgtttg agagatttga gggaagatca cgatttgacc
      901 caaaaacaaa tagctaaaat actttcgttt acaaactcag cttatgctaa aattgaacgg
      961 ggtgagcata cgttaacagc agatgtattg gtaactctct caaactttta cgatgtcagt
     1021 acagactacc tattggaatt gacagatttt cctgataaaa ttcgctttag aaaataatct
     1081 cctcaatttc atagagtgtg aaaatgagga gattttttat ttgttctttg aaaattaaat
     1141 agtccaaaat ggtactttcc tcatttgtgg agcaaatttg aatggctcgc catgataaga
     1201 gcgattttaa aaccatcaat aaaatagagc gatactttat atgccatgat acaaatgata
     1261 tacaatgata cttctgaccg ttcaggctgc catcgtaaaa gagcagcaag tgaaattctt
     1321 atgatgactt catcagtcat gccacgttaa atgtgtgagt ttgttagaga aacgcaatta
     1381 atcctcaaaa ggttccccga accttttgag ttccacagac gcatcacgtg gagtgtgtaa
     1441 gcttgttgct aaaagcgtaa aaaccttgga acgaaaggaa taatagactt cctgcgaaac
     1501 aaaaatacaa tacaataaaa ctatggatga tgaagcaagt aaacaattaa gcgatagccg
     1561 tttcaagagc cttgtaggtg ttcagcgcac gagatgttag ctgtgttaaa aacagcttat
     1621 caacgcaacg taaacgcgca aaaggtggac gaaaaaccaa attaagccta gacgatctcc
     1681 tcatggtaac tattcaatac atgcgagaat agcgcactta tgaacaaatt gcggctgatt
     1741 ttggcattca cgaaagcaac ttaatccgtc ggagccaatg ggttgaagta actcttgttc
     1801 aaaatggttt tacgatttca aattctgccc taattatgaa aaacagtaaa attcgaagga
     1861 ttgtaaggta agagtttttt tctttctgaa aaaatggtat aatagcaatc aaaactagaa
     1921 aataaaacgg aatttggaac agatttgtct gtatcctagt agagtggtga tgctatgaag
     1981 attagtaaga ggcacttatt aaattattcc atcttgattc cctacttgct tttatctatt
     2041 ttgggcttga ttgtggtcta ttcgaccacc agtgctattt taattgaaga aggcaagagc
     2101 gccttgcagt tggttcgaaa ccaaggaatc ttttggattg ttagtttgat actgattgcc
     2161 ttaatttata aattgagact agattttttg agaaatgagc gactaatcat tttagttata
     2221 ttaatagaaa tgcttttatt gttcttggct cgttttattg gtatttccgt aaacggggca
     2281 tacggttgga tttcggttgc aggagtaact attcagccag ctgagtactt aaaaatcatt
     2341 attatttggt atttagctca ccgattctcc aaacagcaag aagaaatagc tacttatgat
     2401 tttcaagttt tgactcaaaa tcaatggctt ccccgtgctt ttaatgattg gcgattcgtt
     2461 ctcctagttc tgattggaag tttgggaatt ttccctgatt taggaaatgc gactatttta
     2521 gtcttggttt ccttgattat gtatacagtt agtggaatcg cttatcgctg gttttcaacc
     2581 attctggcgc tcgtatctgc cgcttctgtc tttgtcttga ccactatcag cctaatcggt
     2641 gttgagacct tttcaaaaat tccagtattt ggctatgtag ccaagcgctt tagtgccttt
     2701 tttaatcctt ttgccgatcg tgctgatgca ggtcaccagt tagctaattc ttattttgcc
     2761 atggtcaatg gtggttggtt tggtctaggt cttggaaact cgattgaaaa acgaggttat
     2821 ttgccagaag ctcatacaga ctttgtcttt tctatcgtga ttgaagaatt tggctttgct
     2881 ggtgccagtc ttattttagc tctcttgttt ttcatgattt tgcggattat cttggtcggt
     2941 attcgagcgg agaatccttt caatgccatg gttgcactcg gtgtcggagg gatgatgttg
     3001 gttcaggtat ttgtcaatat cggagggatt tcgggcttga ttccatctac aggagtaacc
     3061 ttccccttct tatcccaggg tggaaatagt cttctagtct tatcagtggc agtagccttt
     3121 gtcttaaata ttgatgccag tgaaaaacgc gctaagttgt accgagaatt ggaaaatcaa
     3181 ccaatgaacc ttctgttgaa gtaggataaa gaaaggatag tttatgtctc ttcaaaaatt
     3241 agaaaattat agtaataaaa gtgttgtgca agaagaagtc ttgattctaa cagaattact
     3301 ggaagatatt actaaaaata tgcttgcccc agagaccttt gaaaaaataa tacagttgaa
     3361 agaattatca acgcaggaag attatcaagg tctaaaccgt ctagtgacta gcttatcaaa
     3421 tgatgaaatg gtctatattt cacgctattt ctctatctta cctcttttga tcaatatttc
     3481 agaggatgtg gatttagctt atgaaatcaa tcatcaaaat aatattgatc aggactattt
     3541 aggtaaatta tctacaacga ttaaattggt agcagaaaag gaaaatgccg ttgagatcct
     3601 agaacacttg aatgttgtcc ctgttttgac agcccatcca acacaagtgc aacgcaaaag
     3661 tatgttggat ttaacaaatc atattcatag tcttttgcgt aaataccgtg atgttaagtt
     3721 ggggttgatc aataaagata aatggtacaa tgatttgcgt cgttacatcg aaattatcat
     3781 gcagacagac atgattcgtg agaaaaaatt aaaagtgact aacgaaatca cgaatgctat
     3841 ggaatattat aacagctcct ttttgaaagc tgtacctcat ttgacgacgg agtataagcg
     3901 cttagcgcaa gcgcatggtc tgaatttaaa acaggctaaa ccaatcacca tgggtatgtg
     3961 gataggtggt gaccgtgatg gaaatccatt tgttacagca gagaccttga agcagtctgc
     4021 actcactcaa tgtgaagtca tcatgaacta ctatgataaa aagatttacc aactttatcg
     4081 tgaattttct ctttcaacta gcattgtcaa cgtcagcaag caagtcagag aaatggctcg
     4141 tcaatccaag gataactcga tttaccgcga aaaagagctt taccgtcgtg ccttgtttga
     4201 tattcaatca aaaattcagg caactaaaac ctatctgatt gaggatgaag aagttgggac
     4261 tcgttatgaa accgccaatg atttctacaa ggatttgatt gccattcgag attctctact
     4321 agaaaataag ggtgagtcct tgatttcagg tgattttgtg gaattattgc aggcagtaga
     4381 gatatttggt ttttacctag catcaattga tatgcgacaa gactctagcg tctatgaagc
     4441 ctgtgtggca gaactcttga aatcagcagg aattcattct cgttatagcg agttgagcga
     4501 agaagaaaag tgtgaccttc tcttgaaaga attagaagaa gatccccgaa ttctttctgc
     4561 gactcacgca gaaaaatcag aattattagc aaaagaatta gctattttta agacggctcg
     4621 tgttttgaaa gataagttgg gagatgatgt catccgtcag accatcattt cacatgcaac
     4681 cagcctttct gatatgctag aattagctat tctgttaaaa gaagtaggac tggtggatac
     4741 ggaaagggcg cgtgttcaga ttgttcccct ttttgaaaca attgaagact tggatcattc
     4801 agaagaaaca atgagaaaat atctttctct tagccttgcc aaaaaatgga ttgactcacg
     4861 aaataactac caagaaatca tgcttggcta ctctgacagt aataaagatg gcggttactt
     4921 gtcatcatgt tggaccctct acaaggctca acaacaattg actgctattg gagatgaatt
     4981 tggcgttaag gttaccttct tccatggtcg tggtggtact gtcggtcgtg gtggtgggcc
     5041 aacctatgaa gccattacat ctcaaccgct caagtctatc aaggatcgta tccgcttgac
     5101 ggagcagggt gaagtaattg ggaataaata cggtaacaaa gacgccgctt actataacct
     5161 tgaaatgcta gtatcggcag ctattaaccg tatgattact cagaagaaga gcgataccaa
     5221 taccccaaat cgttatgaag ccattatgga tcaagtagtg gaccgtagtt acgatatcta
     5281 ccgtgatttg gtctttggta atgagcattt ctatgattat ttcttcgagt caagtccaat
     5341 caaggctatt tcaagtttta atattggttc tcgtccagcc gctcgtaaga ctattactga
     5401 aatcggtggt ttgcgtgcca tcccttgggt attctcatgg tcacagagtc gtgttatgtt
     5461 ccctggatgg tacggggttg gttcaagctt caaggaattt atcaataaaa atccagagaa
     5521 tattgctatc ttacgagata tgtaccaaaa ttggcctttc ttccaatcgc ttctttcaaa
     5581 tgttgatatg gttttgtcaa aatcaaatat gaatattgct tttgaatatg ctaaactttg
     5641 tgaagacgag caagttaagg ccatctatga gactatttta aatgaatggc aagttactaa
     5701 gaacgttatc ttggctattg aaggacatga cgaactctta gctgacaatc catatctaaa
     5761 agctagtctg gattaccgta tgccttactt taatattctc aactatattc agttagagtt
     5821 gattaaacgc caacgtcgtg gagaattgtc cagtgatcaa gaacgattga ttcatatcac
     5881 catcaacgga attgcgacag gattgcgtaa ttcaggttga taattttcaa gagtgaatgc
     5941 taaaagtgaa tatcaaaaaa attctaatag actattgaca agtagtttaa aaatgatata
     6001 atttaaccat tcagaaaagt aatcatacaa actttttaga gagtctgtgg tagctgaaaa
     6061 cagataagtg gcgatgatga aaattgggct gaatgctatt tagaatttga aattataaaa
     6121 attcggtaag cacaccttac agtgcatctc gttattgcga gactgagcga tagggaaatt
     6181 ccctataatt gaggtggtac cgcgcatcga cgtcctcaca caagtttttt gtgtgaggat
     6241 ttttttgatg gaggttagta tggaaagaaa acgatggcgt cgcttgttta gataagtgaa
     6301 atatgttaaa ggaaataaaa aggagaaaca gaatgaaaaa taaacgttta attggaatta
     6361 ttgctgcatt agcagtctta gtagcaggaa gcttgattta ttcttcaatg aataaatcag
     6421 aagctcagaa taataaggat gagaagaaaa taactaagat tggtgtgctt caatttgtga
     6481 gccatccatc ccttgatttg atttataaag ggatccaaga tggacttgca gaagaaggat
     6541 ataaagatga tcaagttaaa attgatttta tgaactcaga aggtgaccaa agtaaggttg
     6601 cgacaatgag taaacaattg gttgcaaatg ggaatgacct tgtggttggt atcgcaacac
     6661 cagcagccca agggttggct agtgcaacaa aagacctacc ggttatcatg gccgctatta
     6721 cagacccaat tggtgctaac ttggttaaag atttgaaaaa accaggtggc aacattacag
     6781 gggtatctga ccacaatcca gctcaacaac aagttgaact catcaaggct ctgacaccga
     6841 atgtgaaaac aatcggagct ctttactcaa gtagcgaaga caattcaaaa acacaggtcg
     6901 aagaatttaa ggcttatgct gaaaaagcag gtctgacagt ggaaacattt gcagttcctt
     6961 caacaaatga aattgcctca actgtcactg ttatgactag caaggtagat gctatttggg
     7021 ttccaattga taacaccatt gcatcaggat ttccaacggt tgtctctagc aatcaaagtt
     7081 ctaagaaacc aatttatccc agtgcgacag ctatggtaga agtaggtggt ttggcatcag
     7141 ttgtaattga ccaacatgac cttggtgtgg caacaggtaa aatgattgtg caagtcttga
     7201 aaggtgcaaa accagccgat accccagtca atgtcttttc aactggtaag tcagtcatca
     7261 ataaaaaaat agcacaagaa ctaggtatta ctattcctga gtctgttctc aaagaagcag
     7321 gacaagtcat cgaataaata aggtagggag gaggagactc ttcccatttt ttaaaaagat
     7381 gaatatagaa aggattaaat aaaatatgat tgtttccatt atttctcaag gatttgtctg
     7441 ggctattcta ggtctgggaa tctttatgac atttaggatt ttaaactttc cagatatgac
     7501 gacagaaggt tccttccctc ttgggggagc tgttgctgtc actttgataa ccaaaggcgt
     7561 gaacccattt ttagcgacac ttgttgctgt aggagcaggt tgtttggctg gaatggcagc
     7621 aggccttctt tatacaaaag ggaagatccc aaccttgcta tcagggattt tggtgatgac
     7681 ttcttgtcac tcaatcatgc tcttgattat gggacgtgcg aatttaggcc tgcttggaac
     7741 caagcaaatt caggatgttt tgccttttga ttcggatttg aatcaactct tgacaggtct
     7801 catctttgtg agtattgtta ttgctctcat gctctttttc ttggacacta aactcggaca
     7861 agcctatatt gctacagggg ataatcctga tatggctaga agtttcggga ttcatactgg
     7921 acgcatggag ctcatgggct tggtcttatc aaatggtgtg attgcccttg caggtgccct
     7981 cattgctcag caagaaggtt atgccgatgt gtctcgaggg atcggggtta tcgttgtggg
     8041 gcttgcaagt ttgattattg gagaagttat tttcaagagt ttgagcttgg cagagcgttt
     8101 ggttactatc gttgtaggtt ctatcgctta tcaattttta gtgtgggcag ttatcgcact
     8161 tggctttaat acaagttacc ttcgtttata cagtgccttg attttagcag tctgcctcat
     8221 gattccaaca tttaagcaaa caatcttgaa aggagccaag ttaagcaaat gacagcaatt
     8281 gtagaattaa aaaatgcaac caaaatcgtt aaaaatggct ttgatgaaga aaagattatt
     8341 ttaaatgatg tttccttaga aatttttgaa cgggacttta tcacgatttt gggcggaaat
     8401 ggtgctggaa aatcaaccct ctttaacact atagcaggga ccttatcact aactagtgga
     8461 actatccgta ttttaggtga agaactcact aagttttcac ccgagaagcg tgccaagtac
     8521 ctgtctcgtg tcttccaaga tccaaagatg gggacagctc cccgtatgac ggtcgctgaa
     8581 aatcttttaa tcgccaagtt tcgtggtgaa aagcgtggat tgttaccacg acgcttgact
     8641 agctataagg atgaatttca ggcaaccatt gaaaaagtag gaaatggtct tgagaaacac
     8701 ttgaatacac cgattgagtt cttatcaggt ggacaaagac aggctttgag tctcttgatg
     8761 gcaaccttga agcgacctga attactcctg ttagatgagc atactgctgc cctggatcca
     8821 aagactagtg ttgctttgat ggaattgaca gatgaatttg ttaagaaaga tcagctaaca
     8881 gcccttatga ttactcatca tatggaagat gctctcaaat acggcaatcg cttgattgtc
     8941 atgaaagaag gacggattat ccaagattta aaccaagaag aaaaagcaaa aatgaaaatc
     9001 tctgattatt atcaactctt tgaataaagt agggaaaatg agtgaaatgg tttacttttt
     9061 ttctgaaata aagtatacta tataaagtaa actatgataa catggaggta ttgtgtatgg
     9121 ttgacaaaca agtcattgaa gaaatcaaaa acaatgccaa cattgtggaa gtcataggag
     9181 atgtgatttc tttacaaaag gcaggacgga actatctagg gctctgtcct tttcatggtg
     9241 aaaaaacacc ttctttcaac gttgtagagg ataagcagtt ttatcactgt ttcggttgtg
     9301 gtcgctcagg tgatgtcttt aagttcatcg aggagtacca aggggttccc tttatagagg
     9361 cagtccaaat cttaggtcag cgtgttggaa ttgaggttga aaaaccgctt tatagtgagc
     9421 agaagccagc ctcacctcac caagctcttt atgatatgca cgaagatgca gccaaatttt
     9481 accatgcgat tctcatgaca acgactatgg gcgaagaggc cagaaattac ctttatcagc
     9541 ggggtttgac agatgaagtg cttaaacatt tttggattgg tttagcacct ccagaacgaa
     9601 actatctcta tcaacgtttg tctgatcagt atcgtgaaga ggatttactg gattcaggcc
     9661 tgttttatct ctcggatgcc aatcaatttg tagacacctt tcacaatcgc attatgtttc
     9721 ccctgacaaa tgaccaagga aaggtcattg ccttctcagg tcgtatctgg caaaaaacgg
     9781 attcacaaac ttctaagtat aaaaacagcc gatcgactgc aatttttaac aaaagttacg
     9841 aattatatca tatggatagg gcaaaaaaat cttctggaaa agctagtgag atttacttga
     9901 tggaaggatt catggatgtt attgcagcct atcgggctgg aatcgaaagt gctgtggcgt
     9961 cgatgggaac ggccttgagt cgagagcatg ttgagcatct gaaaaggtta accaagaaat
    10021 tggttcttgt ttacgatgga gataaggctg ggcaagccgc gacattgaaa gcattggatg
    10081 aaattggtga tatgcctgtg caaatcgtca gcatgcctga taacttggat cctgatgagt
    10141 atctacaaaa aaatggtcca gaagacttgg cctatctatt aacgaaaact cgtattagtc
    10201 cgattgagtt ctacattcat cagtacaaac ctgaaaacag tgaaaatctg caggctcaga
    10261 ttgagtttct tgaaaaaata gctcccttga ttgttcaaga aaagtccatc gctgctcaaa
    10321 acagctatat tcatatttta gctgacagtc tggcgtcctt tgattatacc cagattgagc
    10381 agattgttaa tgagagtcgt caggtgcaaa ggcagaatcg catggaagga atttccagac
    10441 cgacgccaat caccatgcca atcaccaagc agttatcggc tattatgagg gcagaagccc
    10501 atctactcta tcggatgatg gaatcccctc ttgttttgaa cgattaccgt ttgcgagaag
    10561 actttgcatt tgctacacct gaatttcagg tcttatatga cttgcttggc cagtatggaa
    10621 atcttcctcc agaagtttta gcagagcaga cagaggaagt tgaaagagct tggtaccaag
    10681 ttttagctca ggatttgcct gctgaaatat cgccgcagga acttagtgaa gtagagatga
    10741 ctcgaaacaa ggctctcttg aatcaggaca atatgagaat caaaaagaag gtgcaggaag
    10801 ctagccatgt aggagataca gatacagccc tagaagaatt ggaacgttta atttcccaaa
    10861 agagaagaat ggagtaataa tggcaacaaa acaaaaagaa gtaacaacat ttgacgtaca
    10921 ggtagcagaa tttatccgta atcataagca aaaagggaca gcaacagatg atgaaatcaa
    10981 tgcaagtctg gttattcctt ttaccttgga cgctgatggg attgaagatc tcttgcaacg
    11041 gattcaggat gcaggaattt ctatcacaga taacgaagga aatccaagtg cgcgtgttct
    11101 tagcaatgaa gaagaaccag aactcagcga tgaggacttg attgggtcaa cttctgctaa
    11161 ggtcaatgac cctgtccgta tgtacttgaa agaaataggg gtcgttcctc tcttgaccaa
    11221 tgaagaggag aaagagttgg cactggctgt tgaagctggt gatatcgaag ccaaacaacg
    11281 tcttgcggaa gccaatcttc gtttggttgt ttccattgcc aaacgctatg tcggtcgtgg
    11341 catgcagttc cttgacttga ttcaagaagg aaatatgggc ttgatgaagg cggttgacaa
    11401 gtttgactat tctaaagggt tcaagttttc aacttatgca acttggtgga ttcgtcaggc
    11461 tatcactcgt gctattgcgg accaagctcg taccatccgt atcccagttc acatggttga
    11521 aactatcaat aaattggttc gtgaacagcg gaatctcctt caagaattgg ggcaagatcc
    11581 gacaccagaa cagattgctg aacgaatgga tatgacacct gataaggttc gtgaaatctt
    11641 gaagattgcc caagaaccag tatctcttga aactcctatc ggtgaagagg acgatagcca
    11701 ccttggagac tttatcgaag atgaagtgat tgaaaatcca gtggattata cgactcgtat
    11761 cgtcttgcgt gagcaattgg atgaaatctt agatactctt acagaccgtg aagaaaatgt
    11821 tctgcgtcta cgttttggac tagatgatgg aaaaatgcgc acacttgaag atgtggggaa
    11881 agtctttaac gtaactcgtg agcgtatccg tcagattgaa gcaaaggctt tgagaaaact
    11941 acgccaacca agtcgtagca aaccgcttcg tgattttatt gaagactaag agtgaggaaa
    12001 atatggctta tacagaagag caaattgaaa acatcaaaac acggatttta acagccttgg
    12061 aagaagtcat cgaccctgag ttgggaatcg atattgtcaa tcttggtttg atctatgaga
    12121 ttcgttttga cggtgacaca gggcaaacag agattgacat gactttgaca actatgggtt
    12181 gtcccttagc agaccttttg acagaccaga tttatgatgc tatgatagag gttccagaag
    12241 taacggatac tgaggttaaa ttagtctggt atccagcctg gacagttgaa aaaatgagtc
    12301 gctatgcccg cattgcccta ggcattaagt aaacatataa aagcatgtga gagactgttg
    12361 gaaaagcgag gaaatttccc ctcttttcct ctagtctctc ctttcttttg ctgattttat
    12421 tcaaagaaaa tgatataata gtagttatgg agaaaaagaa attacgcatc aatatgttga
    12481 gttcaagtga gaaagtagca ggacagggag tttcaggtgc ttaccgtgaa ttagttcgtc
    12541 ttcttcaccg tgctgccaag gaccaattga ttgttacaga aaatcttcca atcgaggcag
    12601 atgtgactca ctttcatacg attgattttc cctattattt atcaaccttc caaaagaaac
    12661 gctcagggag aaagattggc tatgtgcatt tcttgccagc tacacttgag ggaagtttga
    12721 aaattccatt tttcttaaag ggaattgtga aacgctatgt attttctttt tacaaccgga
    12781 tggagcactt ggttgtggtc aatcctatgt ttattgagga tttggtagca gctggtattc
    12841 cacgtgaaaa agtgacctat attcctaact ttgtcaacaa ggaaaaatgg catcctctac
    12901 cacaagaaga ggtagtcaga ctgcgcacag atcttggtct tagtgacaat cagtttatcg
    12961 tagtaggtgc tgggcaagtt cagaaacgta aagggattga tgactttatc cgtctggctg
    13021 aggaattgcc tcagattacc tttatctggg ctggtggctt ctcttttggt ggtatgacag
    13081 atggttatga acactataag aaaattatgg aaaatccccc taaaaatttg atttttccag
    13141 gcattgtatc gccagagcgg atgcgcgaat tgtatgctct agcggatctt ttcttgttgc
    13201 ctagttacaa tgagctcttt cctatgacta ttttagaagc tgcgagttgt gaggctccta
    13261 ttatgttgcg tgatttagat ctctataagg taattttgga gggaaattat cgggcgacag
    13321 cgggtagaga agagatgaaa gaggctattt tggaatatca agcaaatcct gctgccttaa
    13381 aagatctcaa agaaaaggct aagaatattt ccagagagta ttctgaagag catctgttac
    13441 aaatctggtt ggacttttat gagaaacaag ccgctttagg gagaaagtaa aaagtgaggt
    13501 aatctatgcg aattggttta tttacagata cctattttcc tcaggtttct ggtgttgcga
    13561 ccagtattcg aaccttgaaa acagaacttg aaaagcaggg acatgctgtt tttatcttta
    13621 cgacgacaga taaggatgtc aatcgctacg aagattggca aattatccgc attccaagtg
    13681 ttcctttctt tgcttttaag gatcgtcgct ttgcctaccg aggttttagc aaggcacttg
    13741 aaattgctaa acagtatcag ctagatatta tccatactca gacagaattt tctcttggcc
    13801 tgttggggat ttggattgcg cgtgaattga aaattccagt catccatacc tatcacaccc
    13861 agtatgaaga ctatgtccat tatattgcta aggggatgtt gatccggccg agtatggtca
    13921 agtatctggt tagaggtttc ctgcatgatg tggatggggt tatttgtcct agtgagattg
    13981 tccgtgactt gctatctgat tataaggtca aggttgaaaa acgggtcatt cctactggga
    14041 ttgaattagc caagtttgag cgtccggaaa tcaagcagga aaatttgaaa gaactgcgta
    14101 gtaaactagg gattcaagat ggtgaaaaga cgttgcttag tctttcgaga atctcctatg
    14161 aaaaaaatat tcaagcagtt ttagcagcct ttgctgatgt tctgaaagag gaagacaagg
    14221 ttaaactggt agtagctggg gatggccctt atctgaatga cctcaaagag caagctcaga
    14281 acctagagat tcaagactca gtcatcttta cagggatgat tgctcctagt gagacggctc
    14341 tttactataa agcggcggat ttcttcattt cggcatcgac aagcgaaacg caaggtttga
    14401 cctacttgga aagcttagcc agtggaacac ctgtcattgc tcacggaaat ccttatttga
    14461 acaacctcat cagtgataaa atgtttggaa ccttgtacta tggagaacat gatttggctg
    14521 gtgctatttt ggaagccctg attgcaacac cagacatgaa cgagcatacc ttatcagaga
    14581 aattgtatga gatttcagct gagaactttg ggaaacgagt gcatgagttt tatctggatg
    14641 ccattatttc aaataacttc cagaaagatt tggctaaaga tgatacggtc agtcagcgta
    14701 tctttaagac agttttgtat cttcagcaac aggtggttgc tgtacctgta aaaggatcta
    14761 gacgcatgtt gaaggcttca aaaacacagt tgatcagtat gagagactat tggaaagacc
    14821 atgaagaata gaaagaggaa cagctatgaa aaaaacaatt aatgagaagc ggtcgtgata
    14881 aaaagattgc gggtgtttgt gctggggtgg cccattatct ggatatggat ccgactatcg
    14941 ttcaagtcat ttggggtgtt cttacttgct gttacggagc tggaattgta gcttacatta
    15001 ttttatggat tatcgcacca gtagcaactg actattgaaa actagctcaa ttcatgctaa
    15061 gataatagaa aatagaatgt aacgaaggag agaaaaatgg catttggaga taatggaaat
    15121 cgtaaaaaaa ctatgtttga gaaaataacc ttgtttatcg tgattatcat gctagtagca
    15181 agtttattgg gaatttttgc aactgcaatt ggtgccctca gtaatctata aaatagattc
    15241 aagaaaattt agtgactggg atttcccagc ccttttttaa agtgagaaga aataatgagt
    15301 atgtttttag atacagctaa gattaaggtc aaggctggta atggtggcga tggtatggtt
    15361 gcctttcgtc gtgaaaaata tgtccctaat ggaggccctt ggggtggtga tggtggtcgt
    15421 ggaggcaatg tggtcttcgt tgtagacgaa ggactacgta ccttgatgga tttccgctac
    15481 aatcgtcatt tcaaggctga ttctggtgaa aaagggatga ccaaagggat gcatggtcgt
    15541 ggtgctgagg accttagagt tcgagtacca caaggtacga ctgttcgtga tgcggagact
    15601 ggcaaggttt taacagattt gattgaacat gggcaagaat ttatcgttgc ccacggtggt
    15661 cgtggtggac gtggaaatat tcgtttcgcg acaccaaaaa atcctgcacc ggaaatctct
    15721 gaaaatggag aaccaggtca ggaacgtgag ttacaattgg aactaaaaat cttggcagat
    15781 gtcggtttag taggattccc atctgtaggg aagtcaacac ttttaagtgt tattacctca
    15841 gctaagccta aaattggtgc ctaccacttt accactattg taccaaattt aggtatggtt
    15901 cgcacccaat caggtgaatc ctttgcagta gccgacttgc caggtttgat tgaaggggct
    15961 agtcaaggtg ttggtttggg aactcagttc ctccgtcaca tcgagcgtac acgtgttatc
    16021 cttcacatca ttgatatgtc agctagcgaa ggccgtgatc catatgagga ttacctagct
    16081 atcaataaag agctggagtc ttacaatctt cgcctcatgg agcgtccaca gattattgta
    16141 gctaataaga tggacatgcc tgagagtcag gaaaatcttg aagaatttaa gaaaaaattg
    16201 tctgaaaatt atgatgaatt tgaagagtta ccagctatct tcccaatttc tggattgacc
    16261 aagcaaggtc tggcaacact tttagatgct acagctgaat tgttagacaa gacaccagaa
    16321 tttttgctct acgacgagtc cgatatggaa gaagaagctt actatggatt tgacgaagaa
    16381 gaaaaagcct ttgaaattag tcgtgatgac gatgcgacat gggtactttc tggtgaaaaa
    16441 ctcatgaaac tctttaatat gaccaacttt gatcgtgatg aatctgtcat gaaatttgcc
    16501 cgtcagcttc gtggtatggg ggttgatgaa gcccttcgtg cgcgtggagc taaagatggg
    16561 gatttggtcc gcattggtaa atttgagttt gaatttgtag actaggagac tggtatggga
    16621 gataaaccga tatctttccg agatgcggat ggtaattttg tttccgccgc agacgtttgg
    16681 aatgaaaaga aattggaaga actatttaat cgtctcaatc caaatcgtgc cttgagattg
    16741 gcacgaacta aaaaggaaaa tccatctcag taaagaagct aaaaaatccc gtgcctcatc
    16801 agacacggga ttttgtggta cgacaggcat gtatagcaaa ctgaatctgg aatagcacag
    16861 catatcttct aaaatattgc tataaattga tttgaatttc aaaattaaat tgttttattc
    16921 ttatttcaat ttgttatagt atatctgatg tcaaagttct cggcgagtca aatagtgatt
    16981 cccaagcctg actatcgtga ggtagcggat taaaatggtc tggggataga ccgttttaag
    17041 tctgatgctg gaaataagaa ttgtcagaag aagggatagc gaaatcgtgg ctctacgaac
    17101 aggaacgtga taataaggcg tatatagcga ataagagggc ctcaaactct aaagtccaaa
    17161 aaggtagtcg taacctatat gcgtaaatca tgagagtaat tgaattcgta ctaagatttt
    17221 ctatttttac tgtaaccttt taacgtcctc atatcttgta tacacgagga aagatgtacg
    17281 acttatcccg tgaggtctat cactataaag agaaaacgac agatagaagt gatcctgagt
    17341 cacggttatc tttaaaatag attgtctgat aggatgctag cctgaaaacg tcttgttcat
    17401 gaaatatagg acggtatgta taaaacgctt ctgtgaactg agagaagggg gagaagttct
    17461 tgctaaaatt tagttgaaca gccgtattcc gatacttaga taagagatct agtcttagct
    17521 cctactcagt ttcaggggat aaaaaagggg caatagcgat tcgagaaaga ttatactctt
    17581 cgaaaatctc ttcaaatcac gtcaatatcg ccttgccgta tgtgtaggat actaactacg
    17641 tcagttccat ctacaatctc aaaacagtgt tttgagcaac ctgcggctag tttcctagtt
    17701 tgatctttga tttttattga gtattagtaa ttcagttact aactcgtcaa ctctgattta
    17761 tccaataaaa ttgaaaagga tggaaaaaag gataaattta tgatatactt tattttgaag
    17821 accttattag aaatcttgaa agagtattga aaacttagaa tgagaaaaat tgttatcaat
    17881 ggtggattac cactgcaagg tgaaattact attagtggtg ctaaaaatag tgttgtggcc
    17941 ttaattccag ctattatatt ggctgatgat gtggtgactt tggattgtgt tccagatatt
    18001 tcggatgtag ccagtcttgt cgaaatcatg gaattaatgg gagctactgt taagcgttat
    18061 gacgatgtct tggagattga tccaagaggt gttcaaaata ttccaatgcc ttatggtaaa
    18121 attaacagtc ttcgtgcatc ttactatttt tatgggagcc tcttaggccg ttttggtgaa
    18181 gcgacagttg gtctaccggg aggatgtgat cttggtcctc gtccgattga cttacacctt
    18241 aaggcgtttg aagctatggg tgccactgct agctacgagg gagataacat gaagttatct
    18301 gctaaagata caggacttca tggtgcaagt atttacatgg atacggttag tgtgggagca
    18361 acgattaata cgatgattgc tgcggttaaa gcaaatggtc gtactattat tgaaaatgca
    18421 gcccgtgaac ctgagattat tgatgtagct actctcttga ataatatggg tgcccatatc
    18481 cgtggggcag gaactaatat catcattatt gatggtgttg aaagattaca tgggacacgt
    18541 catcaggtga ttccagaccg cattgaagct ggaacatata tatctttagc tgctgcagtt
    18601 ggtaaaggaa ttcgtataaa taatgttctt tacgaacacc tggaagggtt tattgctaag
    18661 ttggaagaaa tgggagtgag aatgactgta tctgaagaca gcatttttgt cgaggaacag
    18721 tctaatttga aagcaatcaa tattaagaca gctccttacc caggctttgc aactgatttg
    18781 caacaaccgc ttacccctct tttactaaga gcgaatggtc gtggtacaat tgtcgatacg
    18841 atttacgaaa aacgtgtaaa tcatgttttt gaactagcaa agatggatgc ggatatttcg
    18901 acaacaaatg gtcatatttt gtacacgggt ggacgtgatt tacgtggggc cagtgttaaa
    18961 gcgaccgact taagagctgg ggctgcacta gtcattgctg ggcttatggc tgaaggtaaa
    19021 actgaaatta ccaatatcga gtttatctta cgtggttatt ctgatattat cgaaaaatta
    19081 cgtaatttag gagcggatat tagacttgtt gaggattaaa ccgtagaggt gtttatgaat
    19141 atttggacca aattagcaat gttttctttt tttgaaacgg atcgcttgta tttgcgtcct
    19201 ttctttttta gtgatagtca ggacttccgc gagatagctt caaatccaga aaatcttcaa
    19261 tttattttcc caacgcaggc aagtctggaa gaaagtcaat atgcactggc caattacttt
    19321 atgaagtccc ctttgggagt gtgggcaatt tgtgaccaga aaaatcaaca aatgattggt
    19381 tctattaaat ttgagaagtt agatgaaatc aaaaaagaag ctgagcttgg ctattttttg
    19441 agaaaagatg cttggtcgca aggatttatg acagaggttg ttagaaaaat ttgtcagctt
    19501 tcttttgagg aatttggctt aaaacaatta tttatcatta cccaccttga aaatgaagct
    19561 agccaaagag ttgctcttaa gtctggattt agtttgttcc gtcagtttaa gggaagtgat
    19621 cgttacacaa gaaaaatgcg ggattatctt gaatttcggt atgtaaaagg agagttcaat
    19681 gagtaagcat caggaaattc taagctattt ggaggaatta ccagtaggta aaagggtcag
    19741 tgttcgtagc atttcgaatc atctaggagt tagtgatgga acagcctatc gagctattaa
    19801 agaagctgaa aaccgtggaa ttgtggagac ccgtcctaga agtggaacaa ttcgtgttaa
    19861 atcccagaaa gttgctatag agagattaac gtttgctgaa attgcagaag tgacttcttc
    19921 tgaggttctg gctgggcaag aaggtttaga gagagagttt agtaagtttt caattggtgc
    19981 catgactgaa caaaatatct tgtcttacct tcatgatggg gggctcttga ttgtcggaga
    20041 ccgaacccgt attcagttgc tagccttgga aaatgaaaat gcagttctgg ttacaggggg
    20101 atttcaggtt catgatgatg tgcttaaact ggccaatcaa aaagggattc ctgttctaag
    20161 aagtaagcat gataccttta ccgtcgcgac catgatcaat aaagccttgt caaatgtcca
    20221 aatcaagact gatattctga cagttgagaa actttatcgc cctagtcatg agtatggttt
    20281 tctgagagag acagatacag ttaaagatta tttggacttg gttcgtaaga atcgtagcag
    20341 ccgtttccct gttatcaatc aacatcaggt cgttgttggt gttgtaacca tgagagacgc
    20401 tggtgataaa tcaccaagca cgacaattga taaggttatg tctcgtagtc tattttgggt
    20461 tggattatcg acaaatattg ccaatgtgag tcaacggatg atcgcagaag actttgaaat
    20521 ggtaccagtt gttcgaagca atcaaacttt gcttggcgtt gtgacgcgac gagatgtcat
    20581 ggagaagatg agccgttccc aagtttcggc tctaccaact ttttctgagc agattggaca
    20641 aaagctctct tatcaccatg atgaagtagt cattacagtg gaacccttta tgctagaaaa
    20701 aaatggagtt ttggctaatg gtgtattggc agaaattctg acccacatga cccaagattt
    20761 agttgttaat agtggtcgca atctcattat cgagcagatg ctgatctact ttttgcaggc
    20821 tgttcagata gatgatatat tgcgcattca ggcacggatt attcatcata cgagacggtc
    20881 agctataatt gattacgata tttatcatgg tcaccagatt gtttcaaaag caaatgtgac
    20941 tgttaaaatt aattagaaac taggagaaaa gatgataaca ttaaaatcag ctcgtgaaat
    21001 cgaagctatg gacaaggctg gtgattttct agcaagtatt catataggct tacgtgattt
    21061 gattaagcca ggcgtagata tgtgggaagt tgaagaatat gtccgccgtc gttgtaaaga
    21121 agaaaatttc cttccacttc agattggggt tgacggtgcc atgatggact atccttatgc
    21181 tacctgttgc tctcttaacg atgaagtggc tcacgctttc cctcgtcatt atatcttgaa
    21241 agatggtgat ttgctcaaag ttgatatggt tttgggaggt cccattgcta aatccgacct
    21301 gaatgtctca aaattaaact tcaacaatgt tgaacaaatg aaaaaataca ctcagagcta
    21361 ttctggtggt ttagcagact catgttgggc ttatgctgtt ggtacaccgt ccgaagaagt
    21421 caaaaacttg atggatgtaa ccaaagaagc tatgtacaag ggtattgagc aagctgttgt
    21481 tggaaatcgt atcggtgata tcggtgcggc tattcaagaa tacgctgaaa gtcgtggtta
    21541 cggtgtagtg cgtgatttgg ttggtcatgg tgttggccca actatgcacg aagaaccaat
    21601 ggttcctaac tatggtattg caggtcgtgg actccgtctt cgtgaaggaa tggtcttaac
    21661 cattgaacca atgatcaata caggcgattg ggaaattgat acagatatga aaactggttg
    21721 ggcgcataag accattgacg gtggattgtc atgtcagtat gaacaccaat ttgtcattac
    21781 gaaagatgga cctgttatct tgactagcca aggtgaagaa ggaacttatt aataaaaagt
    21841 gaaaagacta ctggaagttt attttgataa aaaatccagt agatcttttc ataataaaac
    21901 gcattgtatc aagtgttagg agctgatatc atgcgttttt ctgcttttaa gattttttcc
    21961 aactctgttt gtaagcgcat cataacaaag ggtctaggat tcagggctct cctcctatat
    22021 actattagta aagtaaaact aagagaggat attttagtgt cgcagtctat tgttcctgta
    22081 gagattccac aatattgtcg ttttgattct aaaaagagaa atggaattct gtttaacgtt
    22141 cgtattgcca atcttaaatt tacctttttt agattagact tcctgcgaaa
//
LOCUS       DAVLVF010000024        22067 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVLVF010000024 DAVLVF010000000
VERSION     DAVLVF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22067
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.024"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            394..1869
                     /locus_tag="VYN66_000935"
     CDS             394..1869
                     /locus_tag="VYN66_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1494988.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(1835..2550)
                     /locus_tag="VYN66_000936"
                     /pseudo
     CDS             complement(1835..2550)
                     /locus_tag="VYN66_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2806..3666
                     /gene="speE"
                     /locus_tag="VYN66_000937"
     CDS             2806..3666
                     /gene="speE"
                     /locus_tag="VYN66_000937"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV1494989.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            3663..4922
                     /locus_tag="VYN66_000938"
     CDS             3663..4922
                     /locus_tag="VYN66_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV1494990.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            4922..6049
                     /gene="nspC"
                     /locus_tag="VYN66_000939"
     CDS             4922..6049
                     /gene="nspC"
                     /locus_tag="VYN66_000939"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV1494991.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            6046..7131
                     /gene="aguA"
                     /locus_tag="VYN66_000940"
     CDS             6046..7131
                     /gene="aguA"
                     /locus_tag="VYN66_000940"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV1494992.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            7141..8016
                     /gene="aguB"
                     /locus_tag="VYN66_000941"
     CDS             7141..8016
                     /gene="aguB"
                     /locus_tag="VYN66_000941"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV1494993.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGGVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(8197..9006)
                     /locus_tag="VYN66_000942"
     CDS             complement(8197..9006)
                     /locus_tag="VYN66_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1494994.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            9278..9499
                     /locus_tag="VYN66_000943"
     CDS             9278..9499
                     /locus_tag="VYN66_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEV1494995.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCSRGTYDLGYIGGGNHLCKGSAARF"
     gene            9553..10224
                     /locus_tag="VYN66_000944"
     CDS             9553..10224
                     /locus_tag="VYN66_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1494996.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(10276..10473)
                     /locus_tag="VYN66_000945"
     CDS             complement(10276..10473)
                     /locus_tag="VYN66_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1494997.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            10466..11374
                     /locus_tag="VYN66_000946"
     CDS             10466..11374
                     /locus_tag="VYN66_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1494998.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11371..11832
                     /locus_tag="VYN66_000947"
     CDS             11371..11832
                     /locus_tag="VYN66_000947"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV1494999.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            11822..12709
                     /locus_tag="VYN66_000948"
     CDS             11822..12709
                     /locus_tag="VYN66_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1495000.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            12712..14595
                     /gene="cbpE"
                     /locus_tag="VYN66_000949"
                     /gene_synonym="pce"
     CDS             12712..14595
                     /gene="cbpE"
                     /locus_tag="VYN66_000949"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV1495001.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVARHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            14675..15805
                     /locus_tag="VYN66_000950"
     CDS             14675..15805
                     /locus_tag="VYN66_000950"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV1495002.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            15815..17077
                     /locus_tag="VYN66_000951"
     CDS             15815..17077
                     /locus_tag="VYN66_000951"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV1495003.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            17081..17878
                     /gene="proC"
                     /locus_tag="VYN66_000952"
     CDS             17081..17878
                     /gene="proC"
                     /locus_tag="VYN66_000952"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV1495004.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGIVMEAVHQAYKRTQELGK"
     gene            17994..18632
                     /locus_tag="VYN66_000953"
     CDS             17994..18632
                     /locus_tag="VYN66_000953"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV1495005.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTNAVLFDGMGLAK"
     gene            18629..19519
                     /locus_tag="VYN66_000954"
     CDS             18629..19519
                     /locus_tag="VYN66_000954"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV1495006.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLAHQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            19559..19876
                     /gene="yabA"
                     /locus_tag="VYN66_000955"
     CDS             19559..19876
                     /gene="yabA"
                     /locus_tag="VYN66_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV1495007.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            19879..20748
                     /gene="rsmI"
                     /locus_tag="VYN66_000956"
     CDS             19879..20748
                     /gene="rsmI"
                     /locus_tag="VYN66_000956"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV1495008.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            20975..21493
                     /locus_tag="VYN66_000957"
     CDS             20975..21493
                     /locus_tag="VYN66_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV1495009.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            21602..22067
                     /locus_tag="VYN66_000958"
                     /pseudo
     CDS             21602..22067
                     /locus_tag="VYN66_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
BASE COUNT         6631 a         3927 c         5094 g         6415 t
ORIGIN      
        1 aagcttagaa aatgagatga tgttttctag caaatataaa cccgagtaaa aaatgcctac
       61 ggacaggcag ggttgaatgc cgaagcgtgg ttgaaaagcc acattattga tagggttaaa
      121 agcctacttt tataagttga tgttaggaca cttgtcctaa ttcataaatt tttagtgtgg
      181 tgaaagcaca cgtcatcttg tgaaacgatc aataaagtac gtaatatttg ctactagaga
      241 gttaggaaac atcgggaaca gacatactca acagaaacca aaataaacac gtcagaagat
      301 tgcagagcag gtgaaaacct gctctttttt catgagtcaa cctttagttc cttagttttc
      361 ataaggtcct aaaaatattg aaaggagtat gttttgaaag agttagatca aaaccaagcc
      421 ccaatttatg aggccttggt gaagttacgc aagaaaagga ttgttccctt tgatgttcca
      481 ggtcacaagc gtggacgggg aaatccagaa cttgtcgaac tcttaggaga aaaatgtgta
      541 ggcattgatg tcaattcgat gaaacccttg gataatttag gccatcctat ttcgattatt
      601 cgtgatgcag aggagctggc tgcagatgct tttggagcta gccatgcctt tctaatgatt
      661 ggtggaacaa cttcatcggt gcagactatg attctggcaa cctgcaaggc aggagataag
      721 attattctgc cacgaaatgt ccataaatct gctatcaatg cgttggttct atgtggtgcc
      781 attcccatct atatcgagat gagtgtagat cctaagattg gtatcgcttt aggtcttgaa
      841 aatgaccgag tagcacaggc cataaaggac catccagatg ctaaggctat cctaatcaac
      901 aatcctactt actacggcat ctgttcagac ctaaaggggt tgacagaaat ggctcatgaa
      961 gctggcatga tggttttagt agatgaagcc catggagcgc atttgcattt cactggtaaa
     1021 cttccaattt ctgctatgga tgcaggagct gatatggcag cagtttccat gcataagtct
     1081 ggtgggagtt tgacacaaag ttcgcttctt ttaatcgggg agcagatgaa ttctgaatac
     1141 gttcgtcaga taattaacct gactcagtct acatctgcct cttacttgtt gatggctagt
     1201 ttggatattt cacgtcgcaa cttggccctt cgtggtaaag agtcgtttga gaaagtcatt
     1261 gagctatctg agtatgcccg ccgtgaaatc aatgctatcg gtggctacta tgcctactca
     1321 aaagagttaa tagacggtgt ttcggtttgc gattttgacg taactaagct gtcagtttac
     1381 actcagggta ttggcttaac aggtatcgag gtttatgacc tcttgcgaga cgaatacgac
     1441 attcagatcg agtttggtga tatcggcaat atcttggcct atatttccat cggcgaccgc
     1501 atccaagaca tcgagcgctt ggttggtgct ctggctgata ttaagagact ctattcaaga
     1561 gatggaaaag atttgatagc aggagaatat attcagcccg agttggtgct gtctccacaa
     1621 gaagccttct attcagagag aaaaagtttg accttagatg agtctgttgg acaggtctgt
     1681 ggagaatttg tcatgtgcta tcctccaggg attcctatct tggctcctgg tgaacgcatt
     1741 acacgagaaa ttgtagacta tatccaattc gccaaggaac gtggttgctc cctccaaggg
     1801 acggaagatc cagaggtcaa tcatatcaac gttattaaga gaaagacaaa ctataagaaa
     1861 agtcaatagt tttatctaaa ctatttctta tttcaatttg atgatttggc gatgatttta
     1921 gagcacggca aaaagccctt gaattagaag cggtcaatcg cttaatttct atcagcttat
     1981 caaatcctgc ctcaatcctt ttctgaggat tagggtagcg tgtcaagagt tggtaggtat
     2041 attctgaatg ctttccaacg attttatcca actcaggaaa gatgatatca agacaacgag
     2101 tgtattgtac tttccaatca gactgttttt cttgagacga tgaatatgtc tagccagtat
     2161 ttttagttct acttgccgat tatcgtgttg aaattgttca cgattggggt cagaaagaag
     2221 tttaagagcg atgccatgag cgtctttctt atccgtttta gtcttgtgaa gtgataatga
     2281 tttggcaaat tccttgatga gcaaaggatt gtaggtgtaa actttatatc cttgttcatg
     2341 caggaagttc agtagattaa aggcataatg tccggtattt tcaagagcga tgagacagtc
     2401 ttggttgagt tgtcgaagag acagatctaa gagttcaaaa ccagctttat tatttgaaaa
     2461 agtgagtggt ttaagaacag tttttcctgg aacattcaag gctgtaacat cgtgtttatt
     2521 tttagcgaca tcaatgccca cataaagcat gggagtacct ccagatgtag tatttcaagt
     2581 ctacttggtt atccacgaat tttttgcctt gttaccttag acgagataaa acgtctatgc
     2641 gttatcaaac tcattaccaa ttgaaacaaa aaactgtggt tagagccttt cggaaatcgt
     2701 caagcgattg gagggaataa actaatccac agtggcttat tccaagtata ccacttgggc
     2761 tttggcagta gctaactgcg ctaaatataa tataaggaga aatagatgga tttatggttt
     2821 tctgaagttc atactccaga tgtcaaattg tctctgagaa cagccaagca actttacgct
     2881 ggaaaaagtg aatggcagga tatcgaagtc ttggatacgc cagcttttgg gaaaatactg
     2941 attttaaatg gccatgtctt gttctcagat gcggatgatt tcgtctacaa tgaaatgacc
     3001 gttcacgttc ccatggctgt ccacccaaat ccaaagaaag tattggttat tgggggtggt
     3061 gacggcggtg ttgcccaagt attaacactc tatcctgaac tggaacaaat cgatatcgtg
     3121 gaaccggatg agatgttggt cgaggtctgt cgtgagtatt tcccagactt tgctgcaggg
     3181 ctagatgatc ctcgtgttac catttactac caaaatgggc tacgcttttt gcgaaactgc
     3241 gaagatgatt acgatattat catcaacgat gcgacagatc catttggcca tacggaagga
     3301 ctctttacca aggaattcta cggcaatagt tatcgagctc tgaaggaaga cggcatcatg
     3361 atttaccagc atgggagtcc cttctttgac gaggatgagt cggcctgccg aagcatgcac
     3421 cgcaaggtca atcaagcctt tccaatcagt cgggtttatc aggcccatat tccaactagc
     3481 ccagctggct attggttgtt tggatttgca tcgaaaaaat accaccctgt caaagatttt
     3541 gacaaggaag gttggaaaaa acgccagctt ttcacagaat actacactgc aaacttacac
     3601 gtgggatcct ttatgttgcc caagtatgtt gaggacattt tagaagaaga ggaaggaaaa
     3661 aaatgagtcg tttactagtt attggttgtg ggggcgttgc ccaagttgct atttcaaaga
     3721 tttgtcaaga tagcgaaaca tttacagaga ttatgattgc tagccgtacc aagtcaaaat
     3781 gcgatgactt gaaagcgaag ctagaaggca aaacaagtac taaaattgaa actgcagcac
     3841 ttgatgctga caaggttgaa gaagtgattg ccctgattga aagctacaaa ccagaagctg
     3901 ttttgaatgt agctctgcct tatcaagatt taaccattat ggatgcttgt ttggcaacag
     3961 gtgttcacta tatcgataca gccaactacg aagcagaaga cacagaagac cctgagtggc
     4021 gtgctatcta cgaaaaacgt tgtaaggaac ttggttttac agcctacttt gactactcat
     4081 ggcagtgggc ttatcaagag aaattcaaag aagcaggctt gactgctctt cttggttctg
     4141 gttttgaccc aggtgtaact agtgtctttt cagcttatgc cctcaaacac tattttgatg
     4201 aaatccatta tatcgacatt ttagactgta atggcggtga ccacggttat ccatttgcaa
     4261 ccaactttaa tccagaaatt aatctccgtg aggtttctgc gccaggttct tactgggaag
     4321 atgggaaatg ggtcgaagtc gaagctatgt ctatcaagcg tgagtatgat ttccctcaag
     4381 ttggacaaaa agatatgtat ctccttcacc atgaagaaat cgaatcattg gccaagaaca
     4441 ttccaggtgt caaacgcatt cgtttcttta tgacttttgg tcaatcttac ttgacgcaca
     4501 tgaaatgtct tgaaaatgtt ggactccttc gtacggatac cattaacttt aacggccaag
     4561 aaattgttcc aattcaattt ttgaaagcct tgcttccaga tcctgctagc cttggcccac
     4621 gtactgtagg taaaaccaat attggatgta tctttacagg tgtcaaagat ggcgttgaaa
     4681 agactatcta catctacaat gtttgcgacc atcaagagtg ctatgcagaa gtcgggtcac
     4741 aagcaatttc atacacgaca ggcgttccag ccatgattgg gacaaaatta gtcatgaacg
     4801 gaacttggaa acaagctgga gtgtataacc ttgaggagtt agatccagat ccattcatgg
     4861 aagctttgaa tgagtatggt ttgccatggg ttgtggttga aaatccacaa atggtggact
     4921 aatgaagtta gaacaagtac caacaccagc ctatgttatt gacttggcca agttagaagc
     4981 taattgccgc attctacaat atgtacaaga agaggccggt tgcaaggtct tgcttgccca
     5041 gaaggcatat tccctctaca aaacttatcc cttgattagc cagtatctat caggtacgac
     5101 agctagtgga ctctatgagg ccaaattggc aagggaagaa tttcctggtg aagtccatgt
     5161 ttttgcacca gctttcaagg atgaggacat ggaggaattg cttgggataa cagaccatat
     5221 cgtctttaac tcagagagac agttgcgtaa gcatggggct cgttgtcgag aggctggtgt
     5281 cagtgttggt ttgcgcctca accctcagtg ttcaactcaa ggagatcacg cgctctatga
     5341 cccttgtgca cctggttctc gctttggagt tactatagac aagattccga gtgatttgct
     5401 agatttggtt gacggacttc attttcatac cctttgcgag cagggagcag atgatttaca
     5461 aacaactttg aaagcagtag aagaacagtt tggtccctac ttacatgagg taaaatggct
     5521 caatatgggt ggtggtcatc atattacaag agaaggttac gatgtggatt tgctgatttc
     5581 agaaatcaag cgtatccgaa aaacttacaa tcttgaaatc tatatcgagc ctggtgaagc
     5641 cattgcgctt aatgcgggtt atttagcaac tgaggtatta gatattgtag aaaacggtat
     5701 ggaaatcttg gttttagacg cctctgcgac ctgccatatg cctgatgtac ttgagatgcc
     5761 ctatcgtcca cctttgagaa atggctttga gtcacaggaa aaagcccata cctacagact
     5821 ttcttctaat acctgtctga ctggcgatgt gattggtgat tatagttttg aaaatccagt
     5881 ccaaatcgga gacagacttt attttcaaga catggccatt tattcttttg tcaaaaataa
     5941 tacctttaat ggtattggat tgccaagtct ctatctcatg gacgaacagg gagactgtag
     6001 cttactcaaa gcttttggct atcaagactt taaagggaga ttatcatgat ggacagtcca
     6061 aaaaaattag gctatcacat gccagcagag tacgaacccc atcatggtac cctcatgata
     6121 tggccgactc gaccaggatc atggcctttt caaggaaagg ctgctaaaag agcatttact
     6181 cagatcatcg agaccatagc agaaggggaa agagtctatc ttttggtgga gcaggcctat
     6241 ctatctgaag cccaatccta tcttggagac aaggttgttt atttagacat tcccaccaat
     6301 gatgcctggg cgcgtgatac tggcccaacc attctcgtca atgataaagg taagaaatta
     6361 gccgtggatt gggcctttaa tgcttgggga ggcacctatg atggtcttta tcaagattat
     6421 gaagaggatg accaagtagc cagtcgtttt gctgaggcct tggaaaggcc tgtctatgat
     6481 gctaaacctt ttgtactgga aggaggcgca atccatagcg atggtcaagg aactattctc
     6541 gtaactgaaa gttgcttgct tagtcctggt cgcaatccta acctgactaa agaggagatt
     6601 gaaaacacat tattagaaag tcttggtgct gaaaaagtta tttggcttcc ttatggtatt
     6661 tatcaggatg aaaccaatga acacgtcgat aatgttgttg cctttgttgg tcctgctgag
     6721 gttgttttgg cttggacaga tgacgaaaat gatccccagt atgccatgtc aaaagcagat
     6781 ctcgaactct tagaacagga aacagatgca aaaggttgtc acttcaccat tcataaattg
     6841 cctatccctg cagttcgaca agttgtgaca gaagaagatt tgccaggcta catctatgaa
     6901 gaaggagaag aagagcgata cgcaggtgaa cgactagcag cttcctacgt aaacttttat
     6961 atcgccaaca aggctgtctt ggttccacag tttgaggatg taaacgacca agtggcctta
     7021 gatatcctca gcaagtgttt cccagaccgt aaagttgtcg gaataccagc cagagatatt
     7081 ctcttaggtg gtggcaatat ccactgtatc acccaacaaa ttccagaata ggagaaaaag
     7141 atgagaaatg taagagttgc aaccattcag atgcaatgcg ctaaggatgt ggcaacaaat
     7201 atccaaaccg cagagcgttt agtacgtcag gctgctgagc aaggagccca aattattctc
     7261 ttgcccgagt tgtttgaaca tccctatttc tgtcaggaac gtcagtatga ctactaccag
     7321 tatgcccaat ctgtagcgga aaatactgcc attcagcatt ttaaggtgat tgccaaggaa
     7381 ctacaagttg ttttaccaat cagtttctat gaaaaagatg gtaatgtctt gtataactct
     7441 attgccgtca ttgatgcaga tgggggagtg ctgggcgttt atcgaaagac ccatatacca
     7501 gatgaccatt attatcaaga aaaattctat ttcacgcctg gtaacactgg tttcaaggtc
     7561 tggaatactc gctatgctaa gattggtatc ggtatctgtt gggatcaatg gttccctgaa
     7621 acagcccgct gtcttgcatt gaatggtgct gaattgctct tttatcctac agctatcggt
     7681 tcagagccaa ttttggatac agatagttgt ggtcactggc aacgtactat gcaagggcac
     7741 gcagcagcga atattgttcc agtcatcgca gccaatcgtt atggtttaga ggaggttact
     7801 cctagtgagg aaaatggcgg acagagttcc agtcttgact tctacggttc ctcctttatg
     7861 acggatgaaa caggagctat tctagaacga gctgaaagac aagaagaagc tgttctgtta
     7921 gctacttatg acctagacaa gggagcaagt gaacgtctaa actggggctt gtttcgagat
     7981 agaagaccag aaatgtatag acaaattaca gattagtgtg ggagaaatga gagattcatt
     8041 ctgctagact aacttcttat tagcaactat aagatactat ggcatctagt aaatcgattt
     8101 ttatgattcg ctattcttgt ctattgatta gtccgtattt taaaatatta gcaaaaaagc
     8161 aaatagcagt aacttctgtc tatttgcttt tcttttttat agaatatatt tctcaatagc
     8221 acgcgcaacg ccgtcttctt cgttgcttga ggtaacggca tccgcaagag atttgacata
     8281 atcgctggca tttcccattg caatcccaag ccctgcaaac tggagcattt cgatatcgtt
     8341 attagcatcg cccatggcca taatctctga ggaatcaatc ttcaaaatct cagctagtcg
     8401 tgaaagagca gtagcctttg tcgttccaag cggcattgct tcataaatga caggctgcga
     8461 acgaactcca ctgaatcgtt ggcaaagctc ttcagcaaaa cgctgctcaa aatcgtctgt
     8521 ttgttctttt gttcctaaaa acataccttg gaacatccgg aactttccac tagtcgcttc
     8581 ttcaagagaa atttcagtca ggtctgaaaa tactagttta gcatcatttt caataacttg
     8641 attgggtttg ccaccgagaa caaaataatg tgactcgtca aaaagtgtca actgaacatc
     8701 actcttttca gcaaggtcat agaggtattc gatgtcagct ggactcagtt ctttccagtc
     8761 aactagactc caatcactgg tctggtgagt tgaacaaccg ttgttaacaa taatatattc
     8821 gttctggagg tcaagctcca gttttttgta gtaggggagg acaccgaaaa gggggcgacc
     8881 cgtacagaga accagtttga cacctttttc aatggctttg tgaatagcag taatgtgagc
     8941 ttgtgggatt tccttggctt cattgaggag ggtgccgtcc atatccaagg ctagtagttt
     9001 aatcataggt cttccttctt tatcttttgc tattattata gcatattttg gagaagaaat
     9061 tgatagaaag cttgagacta attgatttta tagtttaaga tgttttgatg acaattcatg
     9121 atttgaagag gatatttggc aaagatatgc tatactatgt ttgtcaatgt tgcaactaga
     9181 caaattaaaa aaccaactta atataatagt ttttttgtaa gtaggtatga gtatcagatt
     9241 actcaactaa tctgaagaat aatggaggaa atatatcatg attttaatga caaaaaatat
     9301 aaatctaaca aatgaagaat tagagctgat acaaggtgga gcagattcat atggtaaaaa
     9361 tcctaatggt aggtacgatt gggaaataga accagtatta actctgccgg ttcatggatt
     9421 ttgttccaga ggcacctatg atttaggata tattggagga ggcaatcatc tttgcaaagg
     9481 aagtgctgcg agattttaag taaaatttat tagtaatatg aagaaacaag tggagaaagc
     9541 agagaattta atatgaaaaa acgggctatt caaattttac tagcattgtc cttaattttt
     9601 tacaaatcaa cttggttttg gagagttttc aatcacctcg caaagcccta tctaccagca
     9661 agtcgtgaat tttttcagat tctgcttttg atggagagcg gagttctttt cttagcggtc
     9721 atctatctac tggtttttgc aggaaagaaa atttttcatt tcaagtggca gctgaggtac
     9781 ttcatctacc ttttactggg ctacatcatt tcatatatgt ctgacttcct cttttcgtat
     9841 ttcatatccc tgtcttcaaa tcagatttct ttgaatgaaa cgatagaaat gatggggaga
     9901 caggagttcc cttatttctt gctcatcgtt tgcttcatcg cccctattgc tgaggaattg
     9961 atttatcgag gtgtgcttat gacaaccttt ttcaaaaact caccttggta cggagatgtt
    10021 ttgctttctg ctattatttt cggttatatt catatcaatt ttgctttaac tcctcttgct
    10081 tttttcattt atgctagtgg aggtctgatt ttagctctat tgtatcgcat gactaaaaat
    10141 ctctactatc caatactagt tcatattctc atcaatatca ctgccttctg ggatgtttgg
    10201 ttactcctat tttcaggaag ttagcttact aaaaaaatgt cggaattttc cggcattttc
    10261 ttttttcaca aatagtcaac gtttttcttt ccgatactga agtggtgtgt agccacttat
    10321 ttttttgaat tgattttgaa aataagattg gcgtgagaaa ggcagatagt gaagatagtt
    10381 aagaagaata ggatgttctt ttttcctttt tggaaaactt ctaaaatatg gtataatgaa
    10441 aagataaaga agttgggggt agaagatgaa cattcaacaa ttacgctatg ttgtggctat
    10501 tgccaatagt ggtacttttc gtgaagctgc tgaaaagatg tatgttagtc agccgagtct
    10561 gtctatttct gttcgtgatt tggaaaaaga gttgggcttt aagattttcc gtcggaccag
    10621 ctcagggact ttcttgaccc gtcgtgggat ggaattttat gaaaaagcgc aagaattggt
    10681 taaaggattt gatatttttc aaaatcagta tgccaatcct gaagaagaaa aagatgaatt
    10741 ttccgttgct agccagcact atgacttctt accaccaact attacggcct tttcagagcg
    10801 ctatcctgac tataagaact tccgtatttt tgaatcaact actgttcaaa tattagatga
    10861 agtggcgcaa gggcatagtg agattgggat tatctacctc aacaatcaaa ataaaaaggg
    10921 gattatgcaa cgggttgaaa agttaggtct ggaggtcatc gaattgattc ctttccatac
    10981 ccatatttat ctctgtgagg gtcatccttt agcccagaaa gaggaattag tcatggagga
    11041 tttagcggat ttaccaacgg ttcgtttcac tcaagagaaa gacgagtacc tttattattc
    11101 agagaacttt gtcgatacca gcgctagctc acagatgttt aatgtgacag accgtgccac
    11161 cttgaatggt attttggagc ggacggacgc ctatgcgaca ggttctggat ttttagatag
    11221 tgacagtgtt aatggcatta cagttattcg tctcaaggat aacctagata accgcatggt
    11281 ctatgttaaa cgtgaagaag tggagcttag tcaagctggg actctcttcg tagaagtcat
    11341 gcaagaatat tttgatcaaa agaggaaatc atgaaaaaaa gagcaatagt ggcagtcatt
    11401 gtactgcttt taattgggct ggatcagttg gtcaaatctt atatcgtcca gcagattcca
    11461 ctgggtgaag tgcgctcctg gatccccaat ttcgttagct tgacctacct gcaaaatcga
    11521 ggtgcagcct tttctatctt acaagatcag cagttgttat tcgctgtcat tactctggtt
    11581 gtcgtgatag gtgccatttg gtatttacat aaacacatgg aggactcatt ctggatggtc
    11641 ttgggtttga ctctaataat cgcgggtggt cttggaaact ttattgacag ggtcagtcag
    11701 ggctttgttg tggatatgtt ccaccttgac tttatcaact ttgcaatttt caatgtggca
    11761 gatagctatc tgacggttgg agtgattatt ttattgattg caatgctaaa agaggaaata
    11821 aatggaaatt aaaattgaaa ctggtggtct gcgtttggat aaggctttgt cagatttgtc
    11881 agaattatca cgtagtctcg cgaatgaaca aattaaatca ggccaggtct tggtcaatgg
    11941 tcaagtcaag aaagctaaat acacagtcca agagggtgat gtcgtcactt accatgtgcc
    12001 agaaccagag gtattagagt atgtggctga ggatcttccg ctagaaatag tctaccaaga
    12061 tgaggatgtg gctgtcgtta acaaacctca gggaatggtt gtgcacccga gtgctggtca
    12121 taccagtgga accctagtaa atgccctcat gtatcatatt aaggacttgt cgggtatcaa
    12181 tggggttctg cgtccaggga ttgttcaccg tattgataag gatacgtcag gtcttctcat
    12241 gattgctaaa aacgatgatg cgcatctagt acttgcccaa gaactcaaag ataaaaagtc
    12301 tctccgcaaa tattgggcga ttgttcatgg aaatctgcct aatgatcgtg gtgtaattga
    12361 agcgccgatt ggccggagtg aaaaagaccg taagaaacag gctgtaactg ctaaagggaa
    12421 gcctgcagtg acgcgttttc acgtcttgga acgctttggc gattatagct tagtagagtt
    12481 gcaactggag acagggcgca ctcatcaaat ccgtgtccac atggcttata tcggccattc
    12541 agtcgctggt gatgaggtct atggtcctcg caagactttg aaaggacatg gacaatttct
    12601 tcatgccaag actttaggtt ttactcatcc gagaacaggt aagaccttgg aatttaaagc
    12661 agatatccca gagattttta aggaaacctt ggagagattg agaaagtaag aatgaaaaag
    12721 aaattaacta gtttagcact tgtaggcgct tttttaggtt tgtcatggta tgggaatgtt
    12781 caggctcaag aaagttcagg aaataaaatc cactttatca atgttcaaga aggtggcagt
    12841 gatgcgatta ttcttgaaag caatggacat tttgccatgg tggatacagg agaagattat
    12901 gatttcccag atggaagtga ttctcgctat ccatggagag aaggaattga aacgtcttat
    12961 aagcatgttc taacagaccg tgtctttcgt cgtttgaagg aattgggtgt ccaaaaactt
    13021 gattttattt tggtgaccca tacccacagt gatcatattg gaaatgttga tgaattactg
    13081 tctacctatc cagttgaccg agtctatctt aagaaatata gtgatagtcg tattactaat
    13141 tctgaacgtc tatgggataa tctgtatggc tatgataagg ttttacagac tgctgcagaa
    13201 aaaggtgttt cagttattca aaatatcaca caaggggatg ctcattttca gtttggggac
    13261 atggatattc agctctataa ttatgaaaat gaaactgatt catcgggtga attaaagaaa
    13321 atttgggatg acaattccaa ttccttgatt agcgtggtga aagtcaatgg caagaaaatt
    13381 taccttgggg gcgatttaga taatgttcat ggagcagaag acaagtatgg tcctctcatt
    13441 ggaaaagttg atttgatgaa gtttaatcat caccatgata ccaacaaatc aaataccaag
    13501 gatttcatta aaaatttgag tccgagtttg attgttcaaa cttcggatag tctaccttgg
    13561 aaaaatggtg ttgatagtga gtatgttaat tggctcaaag aacgaggaat tgagagaatc
    13621 aatgcagcca gcaaagacta tgatgcaaca gtttttgata ttcgacaaga cggtttagta
    13681 aacatttcaa cttcctacaa gccgattcca agttttcaag ctggttggca taagagttca
    13741 tatggaaact ggtggtatca agcgcctgat tctacaggag aatatgctgt cggttggaat
    13801 gaaattaagg gtgaatggta ttactttaac caaacgggta tcttattaca gaatcaatgg
    13861 aaaaaatgga acaatcgttg gttctatttg acagactctg gtgttgctgc taaaaattgg
    13921 aagaaaatcg ctggaatctg gtattatttt aacaaagaaa accagatgga aattggttgg
    13981 attcaagata aagagcagtg gtattatttg gatgttgatg gctctatgaa gacaggttgg
    14041 cttcaatata aggggcaatg gtattacttt gctccatcag gggaaatgaa aacgggctgg
    14101 gtaaaagata aagaagcctg gtactatatg gattctactg gtatcatgaa gacaggtgag
    14161 atagaagttg ctagacatca ttactatcta gaagaatcag gagctatgaa gcaaggctgg
    14221 cttaaaaagg caaatgattg gtatttctac aagacagatg gttcacgagc tataggttgg
    14281 attaaagaca aggataaatg gtacttcttg aaagaaaatg gtcaattact tgtgaatggt
    14341 aagacaccag aaggctatac tgtggattca agtggtgcct ggttagtgga tgttccgatc
    14401 gagaaatctg ctacaattaa aactacaagt cattcagaaa taaaagaatc caaagaagta
    14461 gtgaaaaagg atcttgaaaa taaagaaacg agtcaacatg aaagtgttac aaattcttca
    14521 actagtcaag atttgacatc ctcaacttca caaagctctg aaacgagtgt aaacaaatcg
    14581 gaatcagaac agtagtagaa aagaaggttt tagggccttc tttttcctat caactctttt
    14641 ctatttcctg ttattcatgt tataatggat aaatatgaat aatcggagtg agactatgaa
    14701 atacaaacgg attgtcttta aggtgggtac ttcttctctg acaaatgagg atggaagttt
    14761 atcacgtagt aaggtaaagg atattaccca gcagttggct atgctgcacg aggctggtca
    14821 tgagttgatt ttggtgtctt caggtgccat tgcggctggt tttggagcct taggatttaa
    14881 aaagcgtccg actaaaattg ctgataaaca ggcttcagca gcggtagggc agggactttt
    14941 gttggaagaa tatacgacca atcttctctt gcgtcaaatc gtttctgcac aaatcttgct
    15001 gacccaagat gactttgtgg ataagcgtcg ttataaaaat gcccatcagg ctttgtcggt
    15061 tttgctcaac cgtggggcaa ttcctatcat caatgagaat gatagtgtcg ttattgatga
    15121 gctcaaggtt ggggacaatg acactctaag tgctcaagta gcggcgatgg tccaagcaga
    15181 ccttttagtt ttcttgacag atgtggacgg tctctatact ggaaatccta attcagatcc
    15241 aagagccaaa cgcttggaga gaatcgagac catcaatcgt gagattattg atatggctgg
    15301 tggagctggt tcgtcaaacg gaactggggg tatgttaacc aaaatcaagg ctgcaactat
    15361 cgcgacggaa tcaggagttc ctgtttatat ctgctcatcc ttgaaatcag attccatgat
    15421 tgaggcggca gaggagaccg aggatggttc ttactttgtt gctcaagaga aggggcttcg
    15481 tacccagaaa caatggcttg ccttctatgc tcagagtcaa ggttctattt gggttgataa
    15541 aggggctgcg gaagctctct ctcaacatgg aaagagtctt ctcttatctg gtatcgttga
    15601 agcagaagga gtcttttctt acggtgatat cgtgacagta tttgataagg aaagtggaaa
    15661 atcacttgga aaaggacgcg tgcaatttgg agcatctgct ttggaggata tgttgcgttc
    15721 tcaaaaagcc aagggtgtct tgatttaccg tgacgactgg atttccatta ctcctgaaat
    15781 ccaactactt tttacagaat tttagaggta aactatggtg agtagacaag aacaatttga
    15841 acaggtacag gctgttaaaa aatcgattaa cacagctagt gaagaagtga aaaaccaagc
    15901 cttgctagcc atggctgatc acttagtggc tgctactgag gaaattttag cggctaatgc
    15961 cctcgatatg gcagcggcta aggggaaaat ctcagatgtg atgttggatc gtctttattt
    16021 ggatgcagat cgtatagaag cgatggcaag aggaattcgt gaagtggttg ccttaccaga
    16081 tccaatcggt gaagttttag aaacaagtca gcttgaaaat ggtttggtta tcacaaaaaa
    16141 acgtgtagct atgggtgtca tcggtattat ctatgaaagc cgtccaaatg tgacgtctga
    16201 tgcggctgct ttgactctta agagtggaaa tgcggttgtt cttcgtagtg gtaaggatgc
    16261 ctatcaaaca acccatgcca ttgtcacagc cttgaagaag ggcttggaga cgactactat
    16321 tcatccaaat gtgattcaac tggtggagga tactagccgt gaaagtagtt atgctatgat
    16381 gaaggccaag ggctatctag accttctcat tcctcgtgga ggagctggct tgatcaatgc
    16441 agtggttgag aatgcgattg tacctgttat cgagacaggg actgggattg tccatgtcta
    16501 tgtggataag gatgcagacg aagacaaggc gctgtctatc atcaacaatg ctaaaaccag
    16561 tcgtccttct gtttgtaatg ccatggaggt tctgctggtt catgaaaaca aggcagcaag
    16621 catccttcct cgcttggatc aaatgctggt tgcagatcga aaagaagctg ggttggaacc
    16681 aattcaattc cgcctagata gcaaagcaag ccagtttgtt tcaggtcaag ctgctcaagc
    16741 acaagacttt gataccgagt ttttagacta tattctagct gttaaggttg tgagcagttt
    16801 agaagaagcg gttgcgcata ttgaatccca cagtacccat cattcggatg ctattgtgac
    16861 ggaaaatgct gaagctgcag catactttac agatcaagtg gactctgcag cggtgtatgt
    16921 taatgcctca actcgtttca cagatggagg acaatttggt cttggttgtg aaatggggat
    16981 ttctactcag aaattgcacg cgcgtggtcc aatgggcttg aaagagttga ccagctacaa
    17041 gtatgtggtt gctggtgatg ggcagataag ggagtaagag atgaagattg gatttatcgg
    17101 tttggggaat atgggtgcta gcttggcaaa atctgtcttg cagactagga cgtcagatga
    17161 gattctcctt gccaatcgta gtcaagctaa ggtagatgct ttcattgcag actttggtgg
    17221 tcaggcttcc agcaatgaag aaatgtttgc agaagcagat gtgatttttc taggagttaa
    17281 gcctgctcag ttttctgaac tgctttctca atatcagacc atccttgaaa aaagagaaag
    17341 tcttcttttg atttcgatgg cagctggatt gaccttagaa aaactagcaa gtcttatccc
    17401 aagccaacac cgaattattc gtatgatgcc taatacccct gcttctatcg ggcaaggagt
    17461 gattagttat gccttgtctc ctaattgcag ggctgaggac agtgagatct tttgtcagct
    17521 tttagccaag gctggtctct tggttgaatt aggagaaagc ttaatcaatg cagcgacagg
    17581 tcttgcaggt tgtggaccag cctttgtcta tctctttatt gaggccttgg cagatgcagg
    17641 tgttcagacg ggattaccac gagaaacagc cttgaaaatg gcagcccaaa ctgtggtagg
    17701 agctgggcaa ttggtcctag aaagccagca acatcctgga gttttgaaag atcaagtttg
    17761 tagtccaggc ggttcgacta ttgctggcgt agcaagctta gaagcgcatg cttttcgagg
    17821 catagtcatg gaggcagttc atcaagccta taaacgaaca caagaactag gtaaataaga
    17881 ggtagctttg actgcctctt ttatggtggc tgaaatgaga agacacaaaa agattgtcac
    17941 aaactcctat ttttttgata gaatagaagt agtaaaaaag aaatgagtta gacatgtcaa
    18001 aaggattttt agtctctctt gagggaccag agggagcagg caagaccagt gttttagagg
    18061 ctctgctacc aattttagag gaaaaaggag tagaggtgct gacgacccgt gaacctggcg
    18121 gagtcttgat tggggagaag attcgggaag tgattttgga tccaagtcat actcagatgg
    18181 atgctaaaac agagctactt ctctatattg ccagtcgcag acagcatttg gtggaaaaag
    18241 ttcttccagc ccttgaagct ggcaagttgg tcatcatgga tcgttttatc gatagttctg
    18301 ttgcctatca gggatttggt cgtggcttag atattgaagc cattgactgg ctcaatcagt
    18361 ttgcgacaga tggcctcaaa cccgatttga cactctattt tgacatcgag gtggaagaag
    18421 ggctggctcg tattgctgct aatagtgacc gcgaggttaa tcgtttggat ttggaagggt
    18481 tggacttgca taaaaaagtt cgtcaaggct acctttctct tctggataaa gagggaaatc
    18541 gcattgtcaa gattgatgct agtctccctt tggagcaagt tgtggaaact accaatgctg
    18601 tcttgtttga cggaatgggc ttggccaaat gaaacaagat caactaaagg cttggcaacc
    18661 agctcagttt gaccgttttg tccgtatctt agaacaagac cagctcaatc acgcctatct
    18721 cttttcaggt ttctttggaa gcttggaaat ggcgcaattt ttagctaaga gccttttttg
    18781 tacggataaa gttggcgtct taccatgtga gaaatgccga agttgcaagc tgattgaaca
    18841 ggaagagttt ccagatgtca ccttgattaa gccagtcaat caggtcatca agacagaacg
    18901 tattcgggaa ttggtgggac agttttctca agcagggatt gaaagccagc aacaggtctt
    18961 tatcatcgag caagcggata aaatgcatcc caacgcagcc aattctctgc tcaaggtcat
    19021 cgaagaaccc cagagtgaag tttatatttt cttcttgact agcgatgagg aaaagatgtt
    19081 accgacaatc cgaagtcgga ctcagatctt ccactttaaa aagcaagaag aaaaacttat
    19141 cttactctta gaacaaatgg gacttgttaa gaaaaaagcg actcttttag ctaagtttag
    19201 tcaatcgcga gctgaagcag aaaagttggc acatcaggca agtttttgga ccttggtcga
    19261 tgaaagtgaa cgcctgctga cttggttagt agctaagaaa aaagaaagtt atctacaggt
    19321 tgccaaatta gccaacttgg cagatgataa ggaaaaacag gatcaggttt tacggattct
    19381 tgaagttctc tgtgggcagg acctcttgca ggtaagagta agagtgattc tacaagattt
    19441 actagaagct agaaaaatgt ggcaagctaa tgtcagcttt caaaatgcca tggaatatct
    19501 ggtcttgaaa gaaatataaa ctcaaaaatg aatgataaag aaaggaaagg gctgttttat
    19561 ggacaaaaaa gaattatttg acgcgctgga tgatttttcc caacagttat tggtaacctt
    19621 ggccgatgtg gaagccatca agaaaaatct caagagcctg gtagaggaaa atacagctct
    19681 tcgtttggaa aattctaagt tgcgagaacg cttgggtgag gtggaagcag atgctcctgt
    19741 caaggccaag catgttcgtg aaagtgtccg tcgcatttac cgtgatggat ttcacgtatg
    19801 taatgatttt tatggacaac gtcgagagca ggacgaggaa tgtatgtttt gtgacgagtt
    19861 gctatacagg gagtaggcat gcagattcaa aaaagtttta aggggcagtc tccctatggc
    19921 aagctgtatc tagtggcaac gccgattggc aatctagatg atatgacctt tcgagctatc
    19981 cagaccttga aagaagtaga ttggattgct gctgaggata cgcgcaatac aggtcttttg
    20041 ctcaagcatt ttgacatttc caccaagcag atcagttttc atgagcacaa tgccaaggaa
    20101 aaaattcctg atttgattgg tttcttgaaa gcagggcaaa gtattgctca ggtctctgat
    20161 gccggtttgc ctagcatttc agaccctggt catgatttag ttaaggcagc tattgaggaa
    20221 gaaattgcag ttgtgacagt tccaggtgcc tctgcaggaa tttctgcctt gattgccagt
    20281 ggtttagcgc cacagccaca tatcttttac ggttttttac cgagaaaatc aggtcagcag
    20341 aagcaatttt ttggcttgaa aaaagattat cctgaaacac agatttttta tgaatcacct
    20401 catcgtgtag cagacacgtt ggaaaatatg ttagaagtct acggtgaccg ctccgttgtc
    20461 ttggtcaggg aattgaccaa aatctatgaa gaataccaac gaggtactat ctctgagtta
    20521 ttagaaagca ttgctgaaac gccactcaag ggcgaatgtc ttctcattgt tgagggtgcc
    20581 agtcagggtg tggaggaaaa ggacgaggaa gacttgttcg tagaaattca aacccgcatc
    20641 cagcaaggta tgaagaaaaa tcaagctatt aaggaagtag ctaagattta ccagtggaat
    20701 aagagtcaac tctacgctgc ctaccacgac tgggaagaaa aacaataaag ggagatagga
    20761 tgtaataatt ctgtctgttt ctgtttaact taattagtga tgataatata aagatgtatc
    20821 acttggtata gaagctttgg tattaagttt tttattaagc ccatacggaa taccgatggt
    20881 tggagcagca gttatagcgt tcttagaagg tataaataga aaaataaggt cattttaaat
    20941 caaaggattg ataaatcaga aagaaggtga ttttttgcga acatacgaaa ataaagaaga
    21001 actaaaagct gagatagaga aaacatttga gaaatatatt ttagaatttg ataatattcc
    21061 agaaaattta aaagataaga gagctgatga agttgacaga actccagcag aaaaccttgc
    21121 ttatcaggtt ggttggacca acttggttct taaatgggaa gaagatgaaa gaaaggggct
    21181 tcaagtaaaa acaccatcgg ataaatttaa atggaatcaa cttggtgaat tatatcagtg
    21241 gttcacagat acctacgctc atttatctct gcaagagttg aaagcaaaat taaatgaaaa
    21301 tattaattct atctctgcaa tgattgattc gttgagtgag gaagaattat ttgaaccgca
    21361 tatgagaaag tgggctgatg aagcgactaa aacagcgact tgggaagtgt ataagtttat
    21421 tcatgtaaat acggttgcac cttttggaac tttcagaact aaaatcagaa aatggaagaa
    21481 gatagtatta taaattatat ttttaacttt aaaaaatttc ataaaaatgg ttaccaaagg
    21541 cgatagaaga aaaactatcg tctttttctt tgcaaatttt taagaaggga ggtgatcttg
    21601 catggacttt gaatattttt ataacagaga agcggaaaga tttaacttct taaaagtacc
    21661 ggagatatta gttgatagag aagaatttcg gggcttatca gcagaagcaa ttatccttta
    21721 ttccatactt cttaaacgga caggaatgtc atttaagaat aactggatag acaaggaagg
    21781 cagagtattt atctatttta ctgtcgaaga aattatgaaa agaagaaata tctcaaagcc
    21841 aactgccata aaaacattag atgagcttga tgtaaaaaaa aggaatagga ctgatcgaaa
    21901 gagtaaggct tggacttggt aagccgaaca tcatttatgt taaagacttt atgagtatat
    21961 ttcaggtaaa agaaaatgac ttacaaaagt caaaaaactt aacttcagaa gtaaaagatt
    22021 ttaacctcag aagtaaagaa aatgaacttc aagaggttaa gaacctt
//
LOCUS       DAVLVF010000025        21623 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVLVF010000025 DAVLVF010000000
VERSION     DAVLVF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21623
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.025"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            160..2142
                     /locus_tag="VYN66_000959"
     CDS             160..2142
                     /locus_tag="VYN66_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1495010.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKVLDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPILYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2443..7746
                     /locus_tag="VYN66_000960"
     CDS             2443..7746
                     /locus_tag="VYN66_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1495011.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDERDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KQALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKIRDDAHLINAEITVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASVTVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HNPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNVSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSNQGKTPDYKQEID
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(7913..10072)
                     /locus_tag="VYN66_000961"
     CDS             complement(7913..10072)
                     /locus_tag="VYN66_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV1495012.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(10069..10665)
                     /gene="recU"
                     /locus_tag="VYN66_000962"
     CDS             complement(10069..10665)
                     /gene="recU"
                     /locus_tag="VYN66_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV1495013.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10731..11258
                     /locus_tag="VYN66_000963"
     CDS             10731..11258
                     /locus_tag="VYN66_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV1495014.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDELNELAENFSEN"
     gene            11328..11657
                     /gene="gpsB"
                     /locus_tag="VYN66_000964"
     CDS             11328..11657
                     /gene="gpsB"
                     /locus_tag="VYN66_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV1495015.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            11678..12058
                     /gene="rnpB"
                     /locus_tag="VYN66_000965"
     ncRNA           11678..12058
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VYN66_000965"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            12143..13300
                     /locus_tag="VYN66_000966"
     CDS             12143..13300
                     /locus_tag="VYN66_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV1495016.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREMRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLCSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            13313..14707
                     /gene="mapZ"
                     /locus_tag="VYN66_000967"
     CDS             13313..14707
                     /gene="mapZ"
                     /locus_tag="VYN66_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV1495017.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLGKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            14783..16207
                     /gene="gndA"
                     /locus_tag="VYN66_000968"
     CDS             14783..16207
                     /gene="gndA"
                     /locus_tag="VYN66_000968"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV1495018.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPYLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            16219..16908
                     /locus_tag="VYN66_000969"
     CDS             16219..16908
                     /locus_tag="VYN66_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1495019.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGIGYTMQE"
     gene            17006..18028
                     /gene="cbpC"
                     /locus_tag="VYN66_000970"
     CDS             17006..18028
                     /gene="cbpC"
                     /locus_tag="VYN66_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV1495020.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMVTGWYQDGSTWYYLDPSNGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFA
                     SSGEWI"
     gene            18046..19044
                     /gene="cbpJ"
                     /locus_tag="VYN66_000971"
     CDS             18046..19044
                     /gene="cbpJ"
                     /locus_tag="VYN66_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEV1495021.1"
                     /translation="MKILKKTMQVGLTVFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(19162..20394)
                     /locus_tag="VYN66_000972"
     CDS             complement(19162..20394)
                     /locus_tag="VYN66_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1495022.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(20437..21399)
                     /locus_tag="VYN66_000973"
     CDS             complement(20437..21399)
                     /locus_tag="VYN66_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEV1495023.1"
                     /translation="MTSSTEFGNFVSNLSFQHGIAGLLFPLNKLYPPELDSKILSIIK
                     KAVTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANVTAKKLIENYDTL
                     EEIDFALGKSGVLLSLIKYYQFTNDNTLKIFIHNSIGEIYHYFLQRDTAKESILDYSF
                     AHGYCGIAYALFAYSKVLEPSMFYNDLHTFHTELKKLLEKVTSNTENLGNLQLSWCKG
                     ISGIILYLCMYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLM
                     KKIQQVILACSERDDHGLLMFQGDSGKADLFDFGIGSMGYIGVY"
BASE COUNT         6753 a         3850 c         4861 g         6159 t
ORIGIN      
        1 gctccataat atctataggg gatttaccca ctacaaatat tatagagcct ttttttgtct
       61 tttcagaata taccaaatta acacaaaaat tctgaaaatt ctgttgacat ctttctgaaa
      121 agagtttata atggagagaa agttttaaag gagaaaatga tgaaaagttc aaaactattt
      181 gcccttgcgg gcgtgacatt attggcggcg actactttag ctgcatgctc tggatcaggt
      241 tcaagcacta aaggtgagaa gacattctca tacatttatg agacagaccc tgataacctc
      301 aactatttga caactgctaa ggctgcgaca gcaaatatta ccagtaacgt ggttgatggt
      361 ttgctagaaa atgatcgcta cgggaacttt gtgccgtcta tggctgagga ttggtctgta
      421 tccaaggatg gattgactta cacttatact atccgtaagg atgcaaaatg gtatacttct
      481 gaaggggaag aatacgcggc agtcaaagct caagactttg taacaggact aaaatatgct
      541 gctgataaaa aatcagatgc tctttacctt gttcaagaat caatcaaagg gttggatgcc
      601 tatgtaaaag gggaaatcaa agatttctca caagtaggaa ttaaggttct ggatgaacag
      661 acagttcagt acactttgaa caaaccagaa agcttctgga attctaagac aaccatgggt
      721 gtgcttgcgc cagttaatga agagtttttg aattcaaaag gggatgattt tgccaaagct
      781 acggatccaa gtagtctctt gtataacggt ccttatttgt tgaaatccat tgtgaccaaa
      841 tcttctgttg aatttgcgaa aaatccgaac tactgggata aggacaatgt gcatattgac
      901 aaagttaaat tgtcattctg ggatggtcaa gataccagca aacctgcaga aaactttaaa
      961 gatggtagcc ttacagcagc tcgtctctat ccaacaagtg caagtttcgc agagcttgag
     1021 aagagtatga aggacaatat tgtctatact caacaagact ctattacgta tctagttggt
     1081 acaaatattg accgtcagtc ctataaatac acatctaaga ccagcgacga acaaaaggca
     1141 tcgactaaaa aggctctctt aaacaaggat ttccgtcagg ctattgcctt tggttttgat
     1201 cgtacagcct atgcctctca gttgaatgga caaactggag caagtaaaat cttgcgtaat
     1261 ctctttgtgc caccaacatt tgttcaagca gatggtaaaa actttggcga tatggtcaaa
     1321 gagaaattgg tcacttatgg ggatgaatgg aaggatgtta atcttgcaga ttctcaggat
     1381 ggtctttaca atccagaaaa agccaaggct gaatttgcta aagctaaatc agccttacaa
     1441 gcagaaggtg tgacattccc aattcatttg gatatgccag ttgaccagac agcaactaca
     1501 aaagttcagc gcgtccaatc tatgaaacaa tccttggaag caactttagg agctgataat
     1561 gtcattattg atatccaaca actacaaaaa gacgaagtaa acaatattac atattttgct
     1621 gaaaatgctg ctggcgaaga ctgggattta tcagataatg tcggttgggg tccagacttt
     1681 gccgatccat caacttacct tgatatcatc aaaccatctg taggagaaag tactaaaaca
     1741 tatttagggt ttgactcagg ggaagataat gtagctgcta aaaaagtagg tctatatgac
     1801 tacgaaaaat tggttactga ggctggtgat gaggctacag atgttgctaa acgctatgat
     1861 aaatacgctg cagcccaagc ttggttgaca gatagtgctt tgattattcc aactacatct
     1921 cgtacagggc gcccaatctt gtctaagatg gtaccattta caataccatt tgcattgtca
     1981 ggaaataaag gtacaagtga accaatctta tataaatact tggaacttca agacaaggca
     2041 gtcactgtag atgaatacca aaaagctcag gaaaaatgga tgaaagaaaa agaagagtct
     2101 aataaaaagg ctcaagaaga tctcgcaaaa catgtgaaat aactgttgca aaatataaga
     2161 aaggatttag tatttctctt gaatgctgaa tcctttttta catttgtaaa gaaagattct
     2221 aaatgtactg acccccaaaa gttggagcct ctttttgtca gaatagagaa aatttttgtt
     2281 aattttactt gtttcctatt gctttatcag ttattatttg ttatattaaa agtataatta
     2341 ttttttattt atcagggtta agcattgcac tttcagagga aggagtattt tttaaaaagg
     2401 aaatgtaaac gcttgcccaa aaatgaaagg atttagaagt ttatgaataa aggattattt
     2461 gaaaaacgtt gtaaatatag tattcggaaa ttttcattag gtgttgcttc tgttatgatt
     2521 ggagctgcat tctttgggac aagtccggtt cttgcagata gcgtgcagtc tggttctacg
     2581 gcgaatttac cagctgattt agctactgct cttgcaacag caaaagagaa tgatgagcgt
     2641 gattttgaag cgcctaaggt gggagaagac caaggttctc cagaagttac agatggacct
     2701 aagacagaag aagaactatt agcacttgaa aaagaaaaac cggctgaaga aaaaccaaaa
     2761 gaggataaac ctgcagctgc taaacctgaa acacctaaga cggtaacccc tgaatggcaa
     2821 acggtagaga aaaaagaaca acaaggaaca gtcactatcc gagaagaaaa aggtgtccgc
     2881 tacaaccaac tatcctcaac tgctcaaaat gataacgcag gcaaacaagc cctgtttgaa
     2941 aagaagggct tgaccgttga tgccaatgga aatgcaactg ttgatttaac cttcaaagaa
     3001 gattctgaaa agggcaaatc acgctttggt gtcttcttga aatttaaaga taccaataat
     3061 aatgtttttg tcggttatga caaggatggc tggttctggg agtataaatc tccaacaact
     3121 agcacttggt atagaggtag tcgtgttgct gctcctgaaa caggatcaac aaaccgtctc
     3181 tctatcactc tcaagtcaga cggtcagcta aatgccagca ataacgatgt caatctcttt
     3241 gacacagtga ctctaccagc tgcggtcaat gaccatctta aaaatgagaa gaagattctt
     3301 ctcaaggcgg gctcttatga cgatgagcga acagttgtta gcgttaaaac ggataaccaa
     3361 gagggggtaa aaacagagga tacccctgct gaaaaagaaa caggtcctga agttgatgat
     3421 agcaaggtga cttatgacac gattcagtct aaggtcctca aagcagtgat tgaccaagcc
     3481 ttccctcgtg tcaaggaata cagcttgaat ggacatactt tgccaggaca ggtgcaacag
     3541 ttcaaccaag tctttatcaa taaccaccga atcacccctg aagtcactta taagaaaatc
     3601 aatgagacaa cagcagagta cttgatgaag attcgcgatg atgctcactt aatcaatgcg
     3661 gaaataacag tacgcttgca agttgtggac aatcaattgc actttgatgt gaccaagatt
     3721 gtcaaccaca atcaagtcac tccaggtcaa aagattgatg acgaaagaaa actactttct
     3781 tctattagtt tcctcggcaa tgctttagtc tctgtttcta gtgatcaaac tggtgctaag
     3841 tttgatgggg caaccatgtc aaacaatacg catgtcagcg gagatgatca tatcgatgta
     3901 accaatccaa tgaaggattt ggctaagggt tacatgtatg gatttgtttc tacagataag
     3961 cttgctgctg gtgtttggag taactctcaa aatagctatg gtggtggttc gaatgactgg
     4021 actcgtttga cagcttataa agaaacagtc ggaaatgcca actatgtagg aatccatagc
     4081 tctgaatggc aatgggaaaa agcttataag ggcattgttt tcccagaata cacgaaggaa
     4141 cttccaagtg ctaaggttgt tatcactgaa gatgccaatg cagacaagaa agttgattgg
     4201 caagatggtg ccattgctta tcgtagcatt atgaacaatc ctcaaggttg ggaaaaagtt
     4261 aaggatatca cagcttaccg tatcgcgatg aactttggtt ctcaagcaca aaacccattc
     4321 cttatgacct tggatggtat caagaaaatc aatctccaca cagatggtct tgggcaaggt
     4381 gttctcctta aaggatatgg tagcgaaggc catgactctg gtcacttgaa ctatgctgat
     4441 attggtaagc gtatcggtgg tgtcgaagac ttcaagaccc taattgagaa ggctaagaaa
     4501 tatggagctc atctaggtat ccacgttaac gcttcagaaa cttatcctga gtctaaatac
     4561 ttcaatgaaa aaattctccg taagaatcca gatggaagct atagctatgg ttggaactgg
     4621 ctagatcaag gtatcaacat tgatgctgcc tatgacctag ctcatggtcg tttggcacgt
     4681 tgggaagatt tgaagaaaaa acttggtgac ggtctcgact ttatctatgt ggacgtttgg
     4741 ggtaatggtc aatcaggtga taacggtgcc tgggctaccc acgttcttgc taaagaaatt
     4801 aacaaacaag gctggcgctt tgcgatcgag tggggccatg gtggtgagta cgactctacc
     4861 ttccatcact gggcagctga cttgacctac ggtggctaca ccaataaagg tatcaacagt
     4921 gccatcaccc gctttatccg taaccaccaa aaagatgctt gggttgggga ctacagaagt
     4981 tatggtggtg cagccaacta tccactgcta ggtggctaca gcatgaaaga ctttgaaggc
     5041 tggcaaggaa gaagtgacta caatggctat gtaaccaact tatttgccca tgacgtcatg
     5101 accaagtatt tccaacactt cactgtaagc aaatgggaaa atggtacacc ggtgactatg
     5161 accgataacg gtagcaccta taaatggact ccagaaatgc gagtggaatt ggtagatgct
     5221 gacaataata aagtagttgt aactcgtaag tcaaatgatg tcaatagtcc acaatatcgc
     5281 gaacgtacag taacgctcaa cggacgtgtc atccaagatg gttcagctta cttgactcct
     5341 tggaactggg atgcaaatgg taagaaactt tctactgata aggaaaagat gtactacttc
     5401 aatacgcagg ccggtgcaac aacttggacc cttccaagcg attgggcaaa gagcaaggtt
     5461 tacctttaca agctaactga ccaaggtaag acagaagagc aagaactaac tgtaaaagat
     5521 ggtaaaatta ctctagatct tctagcaaat caaccatacg ttctctatcg ttcgaaacaa
     5581 accaatcctg aaatgtcatg gagcgaaggc atgcacatct atgaccaagg atttaacagt
     5641 ggtaccttga aacattggac catttcaggc gatgcttcta aggcagaaat tgtcaagtct
     5701 caaggggcaa acgatatgct tcgtattcaa ggaaacaaag aaaaagttag tctcactcag
     5761 aaattaactg gcttgaaacc aaataccaag tatgccgttt atgtcggtgt cgataaccgt
     5821 agtaatgcca aggcgagcgt cactgtaaat actggtgaaa aagaagtgac tacttatacc
     5881 aataagtctc tcgccctcaa ctatgtaaaa gcctacgccc acaatacacg tcgtaacaat
     5941 gctacagttg acgatacaag ttacttccaa aacatgtacg ccttctttac aactggatcg
     6001 gacgtatcaa atgttactct gacattgagt cgtgaagctg gtgatcaagc aacttacttt
     6061 gatgaaattc gtacctttga aaacaattca agcatgtacg gagacaagca tgatacaggt
     6121 aaaggcacct tcaagcaaga ctttgaaaat gttgctcagg gtatcttccc atttgtagtg
     6181 ggtggtgtcg aaggtgttga agataaccgc actcacttgt ctgaaaaaca caatccatat
     6241 acacaacgtg gttggaatgg taagaaagtc gatgatgtta tcgaaggaaa ttggtcactc
     6301 aagacaaatg gactagtgag ccgtcgtaac ttggtttacc aaaccatccc acaaaacttc
     6361 cgttttgaag caggtaagac ctaccgtgta acctttgaat acgaagcagg atcagacaat
     6421 acctatgctt ttgtagtcgg taagggagaa ttccagtcag gtcgtcgtgg tactcaagca
     6481 agcaacttgg aaatgcatga attgccaaat acttggacag attctaagaa agccaagaag
     6541 gcaaccttcc ttgtgacagg tgcagaaaca ggcgatactt gggtaggtat ctactcaact
     6601 ggaaatgcaa gtaatactcg tggtgattct ggtggaaatg ccaacttccg tggttataac
     6661 gacttcatga tggataatct tcaaatcgaa gaaattaccc taacaggtaa gatgttgaca
     6721 gaaaatgctc tgaagaacta cttgccaacg gttgccatga ctaactacac caaagagtct
     6781 atggatgctt tgaaagaggc ggtctttaac ctcagtcagg ccgatgatga tatcagtgtg
     6841 gaagaagcgc gtgcagagat tgccaagatt gaagctttga agaatgcttt ggttcagaag
     6901 aaaacggctt tggtagcaga tgactttgca agtcttacag ctcctgctca ggctcaagaa
     6961 ggtcttgcaa atgcctttga tggcaatgtg tctagtctat ggcatacatc ttggaatggt
     7021 ggagatgtag gcaagcctgc aaccatggtc ttgaaagaac caactgaaat cactggactt
     7081 cgttatgttc cacgtggatc aggttcaaat ggtaacttgc gtgatgtgaa acttgttgtg
     7141 acagatgagt ctggcaagga gcataccttt actgcaactg attggccaga taacaataag
     7201 ccaaaagaca ttgattttgg taagacaatt aaggctaaga aaattgtcct tacaggtact
     7261 aagacttacg gagatggtgg cgataaatac caatctgcag cggaacttat ctttactcgt
     7321 ccacaggtag cagaaacacc tcttgacttg tcaggctatg aagcagcttt ggctaaggct
     7381 cagaaattaa cagacaaaga caatcaagag gaagtagcta gcgttcaggc aagcatgaaa
     7441 tatgcgacgg ataaccatct cttgacggaa agaatggtgg aatactttgc agattatctc
     7501 aaccaattaa aagattctgc tacgaaacca gatgctccaa ctgtagagaa acctgagttt
     7561 aaacttagct ctttagtttc caatcaaggt aagacgccag attataagca agaaatagat
     7621 agaccagaaa cacctgaaca aatcttgcca gcaacaggtg agagtcaatc tgacacagcc
     7681 ctcttcctag caagtgttag tctagcccta tctgctctct ttgtagtaaa aacgaagaaa
     7741 gactagtatt tagtaaaacc tcttaacaag attacggaag cagtctctat cttttccaat
     7801 gaggtttata gtacagaaaa agcctgagaa gatgtcttct caggcttttg ttaagcacat
     7861 aaatacaata gtgctatgac aaaatcaccc agaaaaatct gggtgataaa tgttatggtt
     7921 gtgctggttg aggattctga ttttgttgat caggggttgt atttgattgt tgcgtattat
     7981 tgttaggatt ggtagtcgta ctattatttg tgcttggagt ggttgagcta gactgtgaag
     8041 ttgaactatc tgatgatgag cttgaacttt cagttgatgg gggttgttgt ggagcaggtg
     8101 agttccacgt agaacgagca ccatttttaa atacgaattc tccatttctg tagagcccct
     8161 ctggtatatt ccaatcctct ggattgcttc cttcagacag ataggtcatc atagagcggt
     8221 aaactttggc agcgaccgta aggccattgc ctacaagtgg tgtcagacgg ttagaatagc
     8281 ctgtccatac agccattgaa tatttacgcg tatagccagc aaatagttca tcaggtgcta
     8341 caaattgaga ggtcttgatg tggttttcaa tttcctcgtc tgtatagtta gaggttcctg
     8401 ttttaccagc ctgagggagc caagcaagat aggcatttcg tccagttcca taactcaaga
     8461 ctgttttcat catgtcggtc atcatatagg ctgtcgtttc cttcatggca cgagttccga
     8521 cattagagaa ctctttttca ctcccatcac taaagacgac tttatggata tacattggtt
     8581 tatagtaagt tccaccattt gcaaaggcag cgtaagcagc agccatcttt tcactacttg
     8641 ctccatattt tttgtctgat tcggttgtgt tacttgaaat ggcatttgag tagtgaatac
     8701 ttgggtagtc gattccgaga ccatttagga aagtcttggc gcggttgagt ccgaccttgt
     8761 ttagagtttc cacggctggg acgtttcgcg attgttgcag ggcgtattgc aaggtgatgt
     8821 tgccaaagta gcccctatcc cagttataaa caggggtatt tgtcccaggg tagttatagg
     8881 gctcatcgtg aacgatagta gcagttgaat cgtagatacc gtactccaag gcaggagcat
     8941 agtctgtgat cggtttcata gttgatcccc agtcgcggtt tgtttctact gcttggttaa
     9001 ttccgaagga aacattactt gactgatggc gtgctcctag ctgggcaatg actttaccgt
     9061 tagaaacatc aacaatggta gaagcgactt gcaattcatc gtctggatag gcaacgtatt
     9121 cgtctgtatt gtaaatatcc cacagatgtt tttgagcttc ttggtctaca tttgtgtaga
     9181 catccatccc agttgtgagc aggttatagc ctgtttcttc ttcaacttga ttgatgactt
     9241 ccttgaggta attatccatg taagcagggt aattacttgc tgatttgaga ctttgtagtc
     9301 catcagtaat tggtgtattg actgctttct catactgttc agcagagatg tagccttgat
     9361 ttttcatttc agataagacc aagtttcggc ggtcttgggc tgcttctgga tgtgaatagg
     9421 ggtcatattg gtttggtgcc tgaggcattc cagccagcaa ggctaactga ggtaaactta
     9481 aattattgag gtctttacca tagtagtttt gagctgctgt ctgcattcca tagttcccat
     9541 tagacatgta gaccttattt atatagtagg tcaagatttc ttgcttggtt gctttttgtt
     9601 ctaactgaat cgctaaccaa gcttcctgag ccttacgaga aatagtctgg tcggaagtcg
     9661 aagttgaaaa gtaagtcaac ttaatcaact gttgggtgag agttgatcca ccttggaggg
     9721 aattgctttg cagattgcgc aagaaagctc ctaggatacg gatggtatca atccccctgt
     9781 ggtcgaagaa gcgatggtct tcgatagaaa cgattgcctt aaccaaatct gtgggaatat
     9841 cattagcttg ggcattgacg cggcgttcag aacccaagtc agcaatgagt tgatttttat
     9901 tgtcgtagat tttactagaa gttgttgcaa ctagtttact ctcggatagg ctaggagcct
     9961 tgctaacgta gtagaaaaaa actcctccgc ctaagacaat ggctgcgata accaagctta
    10021 agaagctaat gctcagatac ttgattaggc gcagaatcgt tggtttgttc atcttgtttt
    10081 accacctaat aaatgttctt tgataacatt gagataagga atttgaggga aggcaccagc
    10141 cttgatttca tatccatatt ctcgaatata ttcaagtggc attgattttt gtcccttatc
    10201 ttgatgatag aagcgaatca aatcgaatgc cggcaataag taggtttctt gctgagaaga
    10261 aaagtgaaga aggacaaagc agattccttg ttgggcaagg acttgttcca tatgctgaat
    10321 ctgatgtgga tgaaaatttt tcatcggaat cgcacgtttt tgttttgttt ccttgacttc
    10381 aaagtcgatg taatatccat tataaacgcc agaatagtcc gtcgttgaag cttgtcgaaa
    10441 ataggcttca acaatcttgg cacgacttcg ttgtggatag tccacttgta cgatttgaat
    10501 aggagttggt ttcttatgta taacagccaa gccctgagac aaatagtagt cgttggtagc
    10561 attgatcatc ttttcaaaag acattcctcg atttgcgaaa tttttgggtt gagaaagaga
    10621 tgtttgtctt ttttgtgatg aaactttatg tggatagttg accataattc tccttattgg
    10681 tacaataaca tcactttatt ataccataat ttgcacagaa agggttaaaa atggctacag
    10741 ctttggtttt aggctattct gcttttgatt tgggtttatt ttctgataag gatcctcgtc
    10801 ttaaattgat taaaaaagcg atacgtaaag atttagaggc tatggcagca gatggggtgt
    10861 cttggcttgt gtttacaggg agtttgggtt ttgaatattg ggttttagag gttgctcagg
    10921 agatgaaaac tgaatacggt ttccagttgg ccaccatttt tgcttttgaa acccatgggg
    10981 aaaattggaa tgaaggcaat cagatgaaac tgagtcgctt taagcaggta gactttgtca
    11041 aatatgccta tcctcgctat gaacacaagg ggcagttaag agattaccag cagtttttgc
    11101 tggaaaacac gactagttcc tatctttttt atgatgaaga aaatgaaaca aaactagctt
    11161 atttttacca aaagatgaaa aatcaagagg actattttat aaagagatta acatttgatg
    11221 aattaaatga acttgctgaa aatttttccg aaaattgaag ctttgacctt gatttttgtt
    11281 tgcctttttt tatataataa tactagcaag cgagaatgga gagagacatg gcaagtatta
    11341 ttttttcagc gaaagatatt tttgaacaag agtttggacg tgaagtccgt ggctataata
    11401 aagtagaagt tgacgagttt ttagacgatg tcatcaagga ctatgaaacc tatgctgcct
    11461 tggtcaagtc acttcgtcag gaaattgcgg atttgaagga agaattaact cgtaaaccga
    11521 aaccttcacc agttcaagca gaaccccttg aagcggcaat tacaagttct atgacgaatt
    11581 ttgatatttt gaaacgcctg aatagattgg aaaaagaagt ttttggtaaa caaattttag
    11641 ataactcaga tttttaagta gttatttgag atgtgcaatt tttggataat cgcgtgagga
    11701 gaattgcttc tcatgaggaa agtccatgct agcacaggct gtgatgcctg tagtgtttgt
    11761 gctaggcgaa accataagcc tagggacgag aaatcgttac ggcagttgaa atggctaagt
    11821 ccttggatag gccagagtag gcttgaaagt gccacagtga cggagtcttt ctggaaacag
    11881 agagagtgga acgcggtaaa cccctcaagc tagcaaccca aattttggtc ggggcatgga
    11941 gtacgcggaa acgaacgtag tattctgact gctatcagct agagctgtta gtggtagaca
    12001 gatgattatc gaaggaagtg gtcctagtca cttctggaac aaaacatggc ttatagaaaa
    12061 ttgcatatag gttggggctg agaaattttc tcaacctcat tttttaaagt ggacatatag
    12121 aaaggtcttg caagactgta acatgaaaaa agaatttaat ttaattgcaa ctgtggcagc
    12181 agggcttgag gctgtcgttg gtcgtgaaat gcgagagttg ggctacgatt gtcaggttga
    12241 aaatggacgt gttcgttttc aaggagacgt gagagctatt atcgaaacca acctttggct
    12301 tcgggcagca gatcgtatca aaattatcgt aggaacgttc ccagctaaga cttttgaaga
    12361 gctatttcag ggagttttcg ctttagattg ggaaaattat ttaccacttg gagctcggtt
    12421 cccgatttca aaagctaaat gtgttaagtc caaacttcac aatgagccca gtgttcaggc
    12481 tatttctaag aaagctgttg tcaagaaatt gcagaaacac tatgctcgcc cagaaggggt
    12541 tcctctgatg gagaatggtc cagagtttaa gattgaggtc tctattctca aagatgtggc
    12601 aactgtcatg attgatacga ccgggtctag cctctttaaa cgtggttatc gtaccgaaaa
    12661 aggtggcgct cctatcaagg aaaatatggc agcagccatt ttacaacttt ctaactggta
    12721 tccagacaag cctttgattg atccgacctg tggttcgggg actttctgta ttgaggcagt
    12781 tatgattgct agaaagatgg cgccaggtct tcgtcgttct tttgcatttg aggaatggaa
    12841 ctggatcagc gatcgcttga ttcaagaagt gcgcacagaa gcggctaaaa aagtagaccg
    12901 tgagcttgag ctggatatca tgggctgtga tattgatgct cgcatggtgg aaattgctaa
    12961 ggccaatgct caggtagctg gtgttgcagg agacattact tttaagcaga tgcgcgtgca
    13021 ggatttatgt tccgataaaa tcaatggagt aatcatttcc aatccgcctt atggtgaacg
    13081 tttgtcagat gatgcagggg tgaccaagct ctatgctgag atggggcaag tatttgcacc
    13141 gctgaaaact tggagcaaat ttatcctgac tagtgatgaa gcttttgaaa gcaagtatgg
    13201 tagccaagca gataagaagc gtaagttata caacggaacc ttgaaagtgg atctatatca
    13261 atattttggt cagcgtgtca aacggcaaga ggtaaaatag aaagggatac tcatgagtaa
    13321 aaaaagacga aatcgtcata aaaaagaagg tcaagaaccg caatttgatt ttgatgaagc
    13381 aaaagagcta acagttggtc aagctattcg taaaaatgaa gaagtggaag caggagtctt
    13441 gcctgaggat tccattttgg acaagtatgt taagcaacac agagatgaaa ttgaggcgga
    13501 taagtttgcg actcgtcaat acaaaaaaga ggagttcgtt gaaactcaga gtctggatga
    13561 tttaattcaa gagatgcgtg aggctgtaga gaagtcagaa gcttcttcgg aggaagttcc
    13621 atcttctgaa gacatcttac tacccttgcc tctggacgat gaggagcaag gcttggatcc
    13681 tctattgcta gatgatgaaa atccaacaga aatgactgaa gaagtggaag aggagcaaaa
    13741 cctttctcgt ctggatcaag aggactcaga aaagaaaagt aaaaaaggct ttattttgac
    13801 cgttttggcg cttgtatcag taattatttg tgtcagtgct tattatgtct accgtcaagt
    13861 ggctcgttcg actaaggaaa ttgaaacttc tcaatcaact acagccaatc aatcggatgt
    13921 ggatgatttt aatacacttt atgacgcctt ttacacagat agcaataaaa cggctttgaa
    13981 aaatagccag tttgataaac tgagtcaact caagacttta cttgataagc tggaaggtag
    14041 tcgtgaacat acgcttgcca aatctaaata tgatagtcta gtaacgcaaa ttaaggccat
    14101 tcaagatgtc aatgcacaat ttgaaaaacc agctattgtg gatggtgtgt tggataccaa
    14161 tgccaaagcc aaatcggatg ctaaatttac agatattaaa actgggaata cggagcttga
    14221 taaagtgcta ggtaaggcta tcagtcttgg taagagccag caaacaagta cttctagctc
    14281 aagttcaagt caaactagca gctcaagctc tagtcaagca agctcaaata cgactagtga
    14341 gccaaaacca agtagttcaa atgagactag aagtagtcgc agtgaagtca atatgggtct
    14401 ctcgagtgca ggggttgctg ttcaaagaag tgccagtcgt gttgcctata atcagtctgc
    14461 tattgatgat agtaataact ctgcctggga ttttgcggat ggtgtcttgg aacaaattct
    14521 agcaacttca cgttcacgtg gctatatcac tggagaccaa tatatccttg aacgtgtcaa
    14581 tatcgttaac ggcaatggtt attacaacct ctacaagcca gatggaacct atctctttac
    14641 ccttaactgt aagacaggct actttgtcgg aaatggcgct ggtcatgcgg atgacttgga
    14701 ttactaagca gtcgttacaa aattctttct tttcaaaagt aaaaatgata aaataaaaca
    14761 aatgaaacaa gaggagtgtc aaatgacaaa agctaacttt ggtgttgtag gtatggccgt
    14821 aatgggtcgt aaccttgccc ttaatattga atcacgtggt tacacagttg ctatctacaa
    14881 ccgtagtaaa gaaaaaacgg aagatgtgat tgcttgccat cctgaaaaga actttgtacc
    14941 aagctatgac gttgaaagtt ttgtaaactc aatcgaaaaa cctcgtcgta tcatgctgat
    15001 ggttcaagct ggacctggta cagatgctac tatccaagcc cttcttccat accttgacaa
    15061 gggtgatatc ttgattgacg gtggaaatac tttctacaaa gataccatcc gtcgtaatga
    15121 agaattggca aactcaggta tcaactttat cggtactggg gtttctggtg gtgaaaaagg
    15181 tgcccttgaa ggtccttcta tcatgcctgg tggacaaaaa gaggcctacg aattggttgc
    15241 ggatgttctt gaagaaatct cagctaaagc accagaagat ggcaaaccat gtgtgactta
    15301 catcggccct gatggagctg gtcactatgt gaaaatggtt cacaatggta ttgagtacgg
    15361 tgatatgcaa ttgatcgcag aaagctatga cttgatgcaa cacttgctag gcctttctgc
    15421 agaggatatg gctgaaatct ttactgagtg gaacaagggt gaattagaca gctatttgat
    15481 cgaaatcaca gctgatattt tgagccgtaa agacgatgaa ggccaagatg gaccaatcgt
    15541 agactacatc cttgatgctg caggtaacaa gggaactggt aaatggacta gccaatcatc
    15601 tcttgacctt ggtgtaccat tgtcactgat tactgagtca gtgtttgcac gctacatttc
    15661 aacttacaaa gaagaacgtg tacatgctag caaggtgctt ccaaaaccag ctgccttcaa
    15721 ctttgaagga gacaaggctg aattgattga aaagatccgt caagcccttt acttttcaaa
    15781 aatcatttca tacgcacaag gatttgctca attacgtgta gcctctaaag aaaacaactg
    15841 gaacttgcca tttgcagata tcgcatctat ctggcgtgat ggctgtatca tccgttctcg
    15901 tttcttgcaa aagattacag atgcttacaa ccgtgatgca gaccttgcca accttctttt
    15961 ggatgaatac ttcttggatg ttactgctaa gtatcaacaa gcagtgcgtg atatcgtagc
    16021 tcttgcggtt caagctggtg tgccagtgcc aactttctca gcagctatta cttactttga
    16081 tagctaccgt tcagctgacc ttccagctaa cttgatccaa gcacaacgtg actactttgg
    16141 tgctcacact taccaacgta aagacaaaga aggaaccttc cactactctt ggtatgacga
    16201 aaaataagta ggtcagccat ggggaaacgg attttattac ttgagaaaga acgaaatcta
    16261 gctcattttt taagtttgga actccagaaa gagcagtatc gggttgatct ggtagaggag
    16321 gggcaaaaag ccctctccat ggctcttcag acagactatg atttgatgtt attgaacgtt
    16381 aatctgggag atatgatggc tcaggatttt gcagaaaaat tgagccgaac taaacctgcc
    16441 tcagtcatca tgattttaga tcattgggaa gacttgcaag aagagctgga agttgttcag
    16501 cgttttgcag tttcatacat ctataagcca gtccttatcg aaaatctggt agcgcgtatt
    16561 tcggcgatct tccgaggtcg ggacttcatt gatcaacact gcagtctgat gaaagttcca
    16621 aggacctacc gcaatcttag gatagatgtt gaacatcaca cggtttatcg tggtgaagag
    16681 atgattgctc tgacccgccg tgagtatgac cttttggcga cacttatggg aagcaagaaa
    16741 gtattgactc gtgagcaatt gttggaaagt gtttggaagt atgaaagtgc gaccgagaca
    16801 aatatcgtag atgtctatat ccgctatcta cggagcaagc ttgatgttaa aggacaaaaa
    16861 agctacatta aaactgtgcg tggtattgga tataccatgc aagaatagaa aagcagttgc
    16921 agttgtgtaa ctgctttttt gaggagtttt tatatattga catgcagtcg ggtctttgct
    16981 acaatcgttt taggaggata tatagatgaa acttttgaaa aaaatgatgc aagttctact
    17041 agcagtcttt ttctttggtt tgctagctac aaatacggta tttgcgaata ccacaggtgg
    17101 ccgatttgtt gataaggata atagaaaata ttatgtaaaa gatgatcata aagcaatcta
    17161 ttggcataaa atagacggta aaacttacta ttttggtgat attggagaga tggttgtcgg
    17221 ttggcaatac ttagaaattc ctggaacagg ttatcgtgat aatttattcg ataaccaacc
    17281 agttaatgaa attggccttc aggagaagtg gtactatttt ggacaagatg gtgctttgct
    17341 agaacaaaca gataaacaag tactagaggc aaaaacgtct gaaaatacag gaaaagtata
    17401 cggtgaacaa tatcctctat ctgctgaaaa gagaacttat tattttgata ataattatgc
    17461 tgtaaagaca ggctggattt atgaagaggg tcattggtat tatttaaata agctaggaaa
    17521 ttttggcgat gattcttaca atccactacc aattggtgaa gttgctaagg gttggactca
    17581 agattttcat gttactattg acattgatag aagcaaacct gctccatggt actacctaga
    17641 tgcttcaggt aagatgctta cagattggca aaaagtaaac ggaaaatggt attattttgg
    17701 ctcctctggt tctatggcaa caggttggaa atatgtacga ggcaaatggt attacttaga
    17761 taataaaaat ggtgatatga aaacaggatg gcaatacctt ggtaacaagt ggtactacct
    17821 ccgttcatca ggagctatgg taactggctg gtatcaagat ggttcaactt ggtactattt
    17881 agatccttct aatggagata tgaaaatagg ttggacaaaa gtaaatggaa aatggtatta
    17941 tctcaattca aatggagcaa tggttacagg tagccaaact atcgatggta aagtttataa
    18001 tttcgcctca tctggtgagt ggatttaata ttggagggta tatagatgaa aattttgaaa
    18061 aaaactatgc aagttggact gacagtattt ttctttggtt tgctagggac cagtacagta
    18121 tttgcagatg attctgaagg atggcagttt gtccaagaaa acggaagaac ctactacaaa
    18181 aagggggacc tcaaagaaac ctactggcga gtgattgatg gtaagtacta ttattttgat
    18241 tctctatctg gagagatggt tgtcggctgg caatatatcc cgtttccatc taaaggtagt
    18301 acaattggtc cttacccaaa tggtatcaga ttagaaggtt ttccaaagtc agagtggtac
    18361 tacttcgata aaaatggagt gctacaagag tttgttggtt ggaaaacatt agagattaaa
    18421 actaaagaca gtgttggaag aaagtacggg gaaaaacgtg aagattcaga agataaagaa
    18481 gagaagcgtt attatacgaa ctattacttt aatcaaaatc attctttaga gacaggttgg
    18541 ctttatgatc agtctaactg gtattatcta gctaagacgg aaattaatgg agaaaactac
    18601 cttggtggtg aaagacgtgc ggggtggata aacgatgatt cgacttggta ctacctagat
    18661 ccaacaactg gtattatgca aacaggttgg caatatctag gtaataagtg gtactacctc
    18721 cgttcctcag gagcaatggc cactggctgg tatcaggaag gtaccacttg gtattattta
    18781 gaccacccaa atggcgatat gaaaacaggt tggcaaaacc ttgggaacaa atggtactat
    18841 ctccgttcat caggagctat ggcaactggt tggtatcaag atggttcaac ttggtactac
    18901 ctaaatgcag gtaatggaga catgaagaca ggttggttcc aggtcaatgg caactggtac
    18961 tatgcttata gctcaggtgc tttggcagtg aatacgaccg tagatggcta ttctgtcaac
    19021 tataatggcg aatgggttcg gtaatgacag ggcgcttata attatattag aacactcgct
    19081 taatcaaaga agtgactttg ttgtacttga cctgacgatt ggggagatta aaagcatgtg
    19141 aaatctacac atgctttttc gttagttatg agtgttgaac ttgactaacc attgacaaaa
    19201 gataagagca attaaaataa gatacaaaaa tatcaatgct aactgcaccg aaacgcccga
    19261 tgctatggat agggctaata gggagagcag ggacggggcg agaatgtcca ctgtgctcag
    19321 agcagagccc accgtcccca tcttgtcact ggggatatgg gcaaagacag ctgcttgtag
    19381 gcgagggctg agaatgccga taatcgtaga actgatgaaa ctggtcatga aaattacgat
    19441 ataggcttga caaaggatac tagttattag agtcaatagg gaaatctcac agaaaaccaa
    19501 gaggtgcgtg aaacggatat ttttaaatag attactgctg gtcatattgc caaggatagc
    19561 tccacaagac atgatgatgg agagcagggc gaggagttgc ccaatgctca gttgtgctag
    19621 tgtctgttgg atgaagcgga gggcaatcag ccgaggggac acatccaaga tggcaatcat
    19681 actggtcgtc agaaacagta gtatgacggt atcttttaac ctcaggagca cagccagcga
    19741 ctgtttaaga tgttgaaaat atttcttaaa actcagtgct gtatgtgaca tttcaattct
    19801 tttttcaacc tcatacaagg tatgtcgaat aacatacagg cccaaaaagg caatgacaaa
    19861 agtcagagtg ttgataaggg aaatagtttg aatacttata acattgataa ggaatgcgcc
    19921 agccagattg gcgacaatac ggacaaccct catcagagac tgcctgaacc ccatggcatc
    19981 atgcaggtcg tccttaatta cactgatgta gagggcgttc atcatgtagg cagacaggta
    20041 gctcaaggtg tcagagatga ggttgatgat gacaatcata atgactgaaa ctaatgaaaa
    20101 gggcgacaag acgagaaata ctgtcaaaat agcgtagagc aggattttga ttttggcaat
    20161 ccagagtgcg tgtttgactc tattttcttg aaaatctcct agaatcccga gaaagagttg
    20221 cgatagtagg ggaaccatct ctgaaatgga aatcagtaaa atcgctagcg gagcgaaatt
    20281 gtactgagcc acataattga taatggcgag gtagaaaata ctgtcgccga atgatgaaaa
    20341 acagctgttt agcagcaact gtctatatat gttgtttgtc caaaataatt tcatactttt
    20401 ctccttatgt ctgcacatcg aatgggaatt tattatttaa tagacaccaa tataccccat
    20461 gcttcctatt ccgaagtcaa acaaatctgc tttaccacta tctccttgaa acataagtaa
    20521 accgtgatca tctcgttcag aacatgctaa aattacctgt tggatttttt tcatcagtaa
    20581 tttgttttga ttgtagacag tggtttgtag taagctagtt attccgtggc aatatcctgt
    20641 catcattttt agatgatggt taaaaacaaa ttcttgatat ttactaataa tatctttgtt
    20701 tccgtcacaa tcgtacatac aaagatataa gattattccg gaaattcctt tgcaccaaga
    20761 aagttgtaaa tttcctaaat tttcagtatt agaagtaact ttttctaata atttttttaa
    20821 ttcagtatgg aatgtatgga gatcattata gaacatagaa ggttctaaga ctttagaata
    20881 ggcaaataaa gcatatgcaa ttccacaata tccatgagca aagctatagt ctaaaatgct
    20941 ttctttggct gtatctcttt gtaggaaata atgataaatt tcccctatac tattgtggat
    21001 gaaaatttta agagtattgt cattggtaaa ttgatagtat tttattaatg ataataggac
    21061 accagatttt cccaatgcaa agtctatttc ctctagagta tcataattct ctattaattt
    21121 tttagcggtg acgtttgcta attgtagata gtattgattt ttactgatag aaaataaatg
    21181 taggagtaac catagatagc ctgcatcacc aaatagcagt gagtattgat attcatatgt
    21241 gtgtgtcgtt ctaattgtca ctgccttctt gatgatagag agtattttag aatccagttc
    21301 tggggggtac aatttattta gaggaaacag tagtccagct attccgtgtt ggaatgataa
    21361 attacttacg aaatttccaa attctgttga agaagtgatc cttccttcag aatttacagt
    21421 tttatctagt aaaaattgta gaacactatt acattctgtt ttgtaatcat atttatgttc
    21481 ctctattttt ttaattttga ctaaattatt tgtttgactt aaatttagta gacattgtaa
    21541 ccaaaatagt tgctcatctg tgagctgacc ttgagtatgt gatagttcta gtaattgaat
    21601 aggatccttt agttagattc aat
//
LOCUS       DAVLVF010000026        20905 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVLVF010000026 DAVLVF010000000
VERSION     DAVLVF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20905
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.026"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            91..2313
                     /gene="recJ"
                     /locus_tag="VYN66_000974"
     CDS             91..2313
                     /gene="recJ"
                     /locus_tag="VYN66_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV1495024.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEERENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GGASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAIVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2488..4149
                     /locus_tag="VYN66_000975"
     CDS             2488..4149
                     /locus_tag="VYN66_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1495025.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4218..4997
                     /locus_tag="VYN66_000976"
     CDS             4218..4997
                     /locus_tag="VYN66_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1495026.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5109..6329
                     /gene="murM"
                     /locus_tag="VYN66_000977"
     CDS             5109..6329
                     /gene="murM"
                     /locus_tag="VYN66_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV1495027.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFVIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6334..7566
                     /gene="murN"
                     /locus_tag="VYN66_000978"
     CDS             6334..7566
                     /gene="murN"
                     /locus_tag="VYN66_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV1495028.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEYFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7645..8346
                     /locus_tag="VYN66_000979"
     CDS             7645..8346
                     /locus_tag="VYN66_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV1495029.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLAGQ
                     FLSVTVRLNHYYLSNPHYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8386..10233
                     /gene="uvrC"
                     /locus_tag="VYN66_000980"
     CDS             8386..10233
                     /gene="uvrC"
                     /locus_tag="VYN66_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV1495030.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGSIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            10223..11056
                     /locus_tag="VYN66_000981"
                     /pseudo
     CDS             10223..11056
                     /locus_tag="VYN66_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(11240..12040)
                     /locus_tag="VYN66_000982"
     CDS             complement(11240..12040)
                     /locus_tag="VYN66_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1495031.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12211..12816
                     /locus_tag="VYN66_000983"
     CDS             12211..12816
                     /locus_tag="VYN66_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV1495032.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            12829..14229
                     /gene="pepV"
                     /locus_tag="VYN66_000984"
     CDS             12829..14229
                     /gene="pepV"
                     /locus_tag="VYN66_000984"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV1495033.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNAIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14283..14861
                     /locus_tag="VYN66_000985"
     CDS             14283..14861
                     /locus_tag="VYN66_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV1495034.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15021..15173
                     /locus_tag="VYN66_000986"
     CDS             15021..15173
                     /locus_tag="VYN66_000986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1495035.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLECAHNLPLSQ
                     LADSYD"
     gene            15216..15308
                     /locus_tag="VYN66_000987"
     CDS             15216..15308
                     /locus_tag="VYN66_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1495036.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            15479..16804
                     /gene="brnQ"
                     /locus_tag="VYN66_000988"
     CDS             15479..16804
                     /gene="brnQ"
                     /locus_tag="VYN66_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV1495037.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRVLTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16902..17939
                     /locus_tag="VYN66_000989"
     CDS             16902..17939
                     /locus_tag="VYN66_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV1495038.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDTRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSEAVS"
     gene            17941..18402
                     /locus_tag="VYN66_000990"
     CDS             17941..18402
                     /locus_tag="VYN66_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1495039.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            18420..19136
                     /locus_tag="VYN66_000991"
     CDS             18420..19136
                     /locus_tag="VYN66_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV1495040.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            19259..19684
                     /gene="rplK"
                     /locus_tag="VYN66_000992"
     CDS             19259..19684
                     /gene="rplK"
                     /locus_tag="VYN66_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV1495041.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            19892..20581
                     /gene="rplA"
                     /locus_tag="VYN66_000993"
     CDS             19892..20581
                     /gene="rplA"
                     /locus_tag="VYN66_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV1495042.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKVKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
BASE COUNT         6278 a         3858 c         4862 g         5907 t
ORIGIN      
        1 aaatgttata aaaaagcaat ctggatagag aaaacgtcta aatcatgtta taatgaagca
       61 atagaattct tagaaggagt ggatgtcttt ttgataacac ctacttatga atggcagttt
      121 gccctgcagg tagaagatgc ggattttaca aagatagcca agaaggctgg actgggtcct
      181 gaggtggctc ggttattgtt tgagagaggg attcaggacc aagaaagtct gaagaagttt
      241 ttagaacctt ccttggagga cttacatgat gcttatctgc tccatgatat ggacaaggca
      301 gtggagcgga ttcgtcaggc tattgaagaa agggaaaata ttctcgttta tggagactac
      361 gatgcggatg gcatgacttc ggcttctatt gtgaaggaaa gtttggaaca acttggtgcg
      421 gagtgccgag tttacctgcc aaatcgtttt accgatggct atggccctaa tgctagtgtt
      481 tataaatact ttatcgagca agaaggaatt tccttgattg tgacggtgga caatggggtt
      541 gctggtcatg aggctattgc attggctcag tctatgggag tagatgtcat tgtgacagac
      601 catcattcca tgcctgaaac cctgccagat gcttatgcta ttgtccatcc tgaacatcca
      661 gatgcggatt atccttttaa atatttggct ggttgtggag ttgctttcaa gttggcttgt
      721 gccctgttag aagaagtgca agtggaattg cttgatttgg tcgctattgg aactattgca
      781 gatatggtga gtctgacgga tgaaaatcgt atcttagttc aatatggtct ggaaatgttg
      841 ggtcataccc agcgcattgg tctgcaagaa atgctggaca tggctgggat tgctgccaac
      901 gaagtaacag aagaaacggt tggtttccag attgctcctc gtttgaatgc cttgggtcgc
      961 ttggatgatc ccaatcctgc cattgatttg ttgactggat ttgatgatga ggaagcgcat
     1021 gagattgccc ttatgattca ccagaaaaac gaagagcgca aggaaatcgt tcagtctatc
     1081 tatgaagaag ccaagaccat ggtggatcct gagaagaagg ttcaggtctt ggccaaggaa
     1141 ggctggaatc ctggggttct aggaatcgtg gctggtcgtt tattggaaga attgggacag
     1201 acagtcattg ttcttaatat agaagacggt cgtgccaagg gcagtgctcg tagtgtggaa
     1261 gcggtcgata tttttgaagc tctggatccc catcgagacc tcttcatcgc ctttggaggt
     1321 catgcaggtg cagcgggtat gacgctggaa gttgagcaac tctcagattt atctcaggtt
     1381 ttggaagatt atgttcgtga aaaaggtgca gatgctggtg gcaagaataa gttaaaccta
     1441 gatgaagagt tggatttgga ggcacttagc ttggaaacgg tcaaaagttt tgaacgttta
     1501 gctccttttg gaatggataa tcagaaacct attttttata tcaagaattt tcaggtcgaa
     1561 agtgctcgta ctatgggggc aggtaatgcc catctaaagc tgaaaatttc caagggtggg
     1621 gcgagttttg aagtggtagc ctttggtcaa ggcagatggg cgacagagtt ttctcaaacc
     1681 aagaatctag agttagcggt taaattgtct gtcaaccaat ggaatggcca aactgccctc
     1741 cagttgatga tggtggatgc gcgagtggaa ggtgttcaac tttttaacat tcgtggaaaa
     1801 aatgcagtct tgccagaagg tgttccagtc ttggattttc ctggagaact gccaaatctt
     1861 gcggctagtg aagctattgt cgtaaaaaac attccagagg atattactca gctgaagacc
     1921 atttttcagg aacagcattt ctctgctgtc tatttcaaaa atgatattga caaggcctat
     1981 tatctgacag gttatgggac tagagatcag tttgccaaat tgtacaagac tatttaccag
     2041 ttcccagagt ttgatattcg ctacaagctg aaagatttgg ctgcatatct taatattcaa
     2101 caaatcttgc tggtcaagat gattcaagta tttgaagaac taggctttgt gacgataaaa
     2161 gatggtgtga tgacagtcaa taaagaggcg ccaaagcggg agataggaga aagtcaaatt
     2221 taccaaaatc tcaaacaaac cgttaaagac caagaaatga tggcgctggg tacggtgcaa
     2281 gaaatttatg attttttgat ggaaaaagag tagaagttag gaaagagttg ggaaatcaac
     2341 tcttttttga aaacagacct tcattttgaa aatcatcaaa aaaatggtat aatggtagga
     2401 aaagattcgg ctgaaagtat tagaactttt agaataagag ggtagagttg ccctataatc
     2461 aagataaact aagattttgg aggaaaaatg agtaatatca gtttaacaac acttggtggt
     2521 gtgcgtgaga atggaaaaaa tatgtacatt gctgaaattg gagagtccat ttttgttttg
     2581 aatgtagggt taaaatatcc tgaaaatgaa caattagggg tcgatgtggt gattccaaac
     2641 atggattacc tttttgaaaa tagcgaccgt attgctgggg ttttcttgac ccacgggcat
     2701 gcggatgcca ttggtgctct accttatctc ttggcagagg ctaaagttcc tgtatttggg
     2761 tctgagttga ccattgagtt ggcaaagctc tttgtcaaag gaaatgatgc cgttaagaaa
     2821 tttaatgatt tccatgtcat tgatgagaat acggagattg attttggtgg gacagtggtt
     2881 tccttcttcc ctacgactta ctccgttcca gagagtctgg gaattgtctt gaagacatcg
     2941 gaaggaagca tcgtttatac aggtgacttc aaatttgacc aaacggctag tgaatcttat
     3001 gcaactgatt ttgctcgttt ggcagagatt ggtcgtgacg gcgtcctggc tctcctcagt
     3061 gattcggcca atgcagacag caatattcag gtggctagtg aaagtgaagt tagggatgaa
     3121 attacccaaa ctattgctga ctgggaaggt cgtatcatcg ttgcagctgt ttccagtaat
     3181 ctttctcgta ttcagcagat ttttgacgct gcggataaaa caggtcgacg tatcgtcttg
     3241 acaggatttg atattgaaaa tatcgtccgc acagcgattc gtcttaagaa gttgtcttta
     3301 gccaacgaaa ttctcttgat taagcctaaa gatatgtctc gctttgaaga ccatgagttg
     3361 attattcttg agacaggtcg tatgggtgaa cctatcaatg gacttcgtaa gatgtcgatt
     3421 ggtcgccatc gttatgtaga aatcaaggat ggggacctgg tctatattgc tacggctccg
     3481 tctattgcta aagaagcctt tgttgcgcgt gtggaaaata tgatttatca ggcaggtggg
     3541 gttgtcaaat tgattaccca aagtttacat gtatcagggc acggaaatgt gcgtgatttg
     3601 cagctgatga tcaatctttt gcaacctaag tacctcttcc ctgtccaagg ggagtatcgt
     3661 gagttggatg ctcacgctaa ggctgccatg gcagttggga tgttgccaga acacatcttc
     3721 attcctaaaa aggggacgac catggcttac gagaatggag actttgttcc agctggatcg
     3781 gtttcagcag gagatatctt gattgatggg aatgccattg gtgatgttgg aaatgttgtt
     3841 cttcgtgacc gtaaggtctt gtcagaggat ggaattttca tcgtggctat tacagtcaac
     3901 cgtcgtgaga agaaaattgt ggctagggct cgtgttcata cgcgcggatt tgtttatctc
     3961 aagaagagtc gcgatattct ccgtgaaagt tcagaattga ttaaccaaac ggtagaagag
     4021 tatcttcaag gagatgactt tgactgggca gatcttaaag ggaaggttcg agataatttg
     4081 accaagtatc tctttgacca aaccaagcgt cgtccagcta ttttaccagt agtcatggaa
     4141 gcaaaataat cgttgaaata aacagagaga aagtcgagtt tcggcttttt cttatagaaa
     4201 aatagaagga gaaaatcatg gcagtaatga aaatcgagta ttactcacaa gtattggata
     4261 tggagtgggg ggtgaatgtc ctctaccctg atgccaatcg agtggaagaa ccagagtgtg
     4321 aagatattcc cgtcttgtac cttttgcacg ggatgtctgg aaatcataat agttggctta
     4381 agcggaccaa tgtagaacgc ttgcttcgag gaactaatct catcgttgtt atgcccaata
     4441 ccagcaatgg ttggtacacc gatacccagt atggttttga ctactacacg gctctagcag
     4501 aggaattgcc acaggttctg aaacgcttct tccctaatat gactagcaag cgtgaaaaga
     4561 cctttatcgc tggtctttct atgggaggct acggctgctt caaactggct cttgcgacaa
     4621 atcgtttttc tcatgcagct agcttttcag gtgccctcag ctttcaaaac ttttctcctg
     4681 aaagtcaaaa tctgggaagt ccagcctact ggagaggtgt ttttggagag attaaagact
     4741 ggacaactag tccctattct cttgaaagtc tggctaaaaa atcggataaa aagaccaaac
     4801 tttgggcgtg gtgtggcgaa caggatttct tgtacgaagc caataatctc gcagtgaaaa
     4861 atctcaaaaa actaggtttt gatgtgacct atagccatag cgctggaact cacgagtggt
     4921 actactggga aaaacaattg gaagtttttt taacaaccct accaattgat ttcaaattag
     4981 aagagagact gacttagttt gaacttcagc atagggggag tagaactaaa ataaaatatg
     5041 ttttcactag acttttcaaa cgaaagtagt agaatagtaa taaaatactg gaggaaagag
     5101 agtaggaaat gtaccgttat caaattggca ttcccacatt agaatatgat cagtttgtca
     5161 aagaacatga attagccaat gtattacaaa gtagtgcttg ggaggaagtt aagtctaatt
     5221 ggcaacatga gaagtttggt gtttacaggg aagaaaaatt actggcgaca gctagtattt
     5281 tgattagaac tcttccgcta ggctataaaa tgttttacat cccaagagga cctatattgg
     5341 attatgggga taaagaactc ttgaattttg tcattcagtc tattaagtcc tatgctcgca
     5401 gtaagagagc ggtttttgtg acttttgacc caagtatttg cctatctcaa agtttaatca
     5461 atcaggaaaa gacagaattt cctgaaaatc tggctattat tgatagtttg caacaaatgg
     5521 gagtaaggtg gtcaggaaaa acggaggaaa tgggagacac cattcaacct cgtattcagg
     5581 cgaaaatata caaggaaaat tttgaagaag ataaactttc caagtcaaca aaacaggcta
     5641 ttcgaacagc acgaaacaaa gggcttgaga ttcaatatgg tggactggaa ctattagatt
     5701 cattttcgga gttgatgaaa aaaactgaga agcgaaaaga gattcatttg aggaatgaag
     5761 cctattataa aaaattgtta gataatttta aggacaaggc ctatatcacc ttggccacct
     5821 tggatgtttc taaacgttcg caagagttag aagaacagtt agcgaaaaat agagccttgg
     5881 aagagacgtt tactgagtcg actcgaactt caaaagtaga agcgcagaag aaggaaaaag
     5941 aacgtttgtt agaggaattg accttcttgc aggaatatat agatgtaggt caagcgagag
     6001 ttcctttagc ggctactttg agtttggaat ttggtactac ctctgtcaat atatatgctg
     6061 gtatggatga tgattttaaa cgttacaatg caccaatttt aacatggtat gaaacggctc
     6121 gctatgcctt tgagcgaggt atggtctggc aaaatttagg tggtgttgaa aactctctca
     6181 atggtggact ttatcatttt aaggaaaaat ttaatccaac gattgaagaa tacttgggtg
     6241 aatttacaat gcccactcat cctctctatc ctctgttaag acttgctctt gatttccgta
     6301 aaacattaag aaaaaaacat agaaagtaag tatatggcac taacaacact cacgaaagaa
     6361 gagtttcaga cttattctga tcaggtttct tctcgttcct ttatgcaatc tgtccagatg
     6421 ggggatttgc tagaaaaaag aggggctcga attgtttatc ttgctttgaa acaagaagga
     6481 gaaattcaag ttgcagctct ggtttatagc ctgcccatgc tgggtggtct gcatatggaa
     6541 ctcaattcgg ggccgattta tacccaacaa gatgctcttc cagtttttta tgcagagtta
     6601 aaagaatatg ccaagcaaaa tggtgtatta gagttgcttg taaaacctta tgaaacttat
     6661 caaacttttg atagccaagg taatccaata gatgctgaga aaaaaagtat tattcaaggt
     6721 ttgactgatt taggttatca atttgatggc ttaacaacag gttacccagg tggagaacca
     6781 gattggttat actataaaga tttaactgaa ttaactgaaa agagtttgct taaaagtttt
     6841 agcaaaaagg gtaaaccctt ggtgaaaaag gctgaaacct ttggcattcg gttgaaaaag
     6901 ttaaaacgtg aagaactatc gatttttaag aatataacaa aagaaacctc tgaacgtaga
     6961 gaatatagtg ataaaagttt agaatattat gagtattttt atgatacttt tggagaacaa
     7021 gcggagtttc tcatagcaag cttgaatttt tcggagtata tgagcaaatt gcaaggtgaa
     7081 caaagtaaac tagaagaaaa cttggacaag ttgcgacttg atttgagtaa aaatcctcat
     7141 tctgagaaaa aacaaaatca actgagagaa tattctagtc aatttgaaac gtttgaagtt
     7201 cgaaaagcag aagcgcgaga cttgattgaa aaatatggag aagaagatat tgttttagct
     7261 gggagtttat ttgtttatat gcctcaggaa acgacttatc tctttagtgg ttcctacact
     7321 gagtttaata agttctatgc ccctgcactg cttcaaaaat atgttatgtt ggaaagcata
     7381 aaacgtggaa tacctaaata caatttccta ggcattcaag ggatttttga tggaagtgat
     7441 ggtgttttgc gttttaaaca gaattttaat ggctatattg tacgcaaagc gggtactttc
     7501 cgttaccatc catcgccttt aaaatacaaa gctatccagt tactcaaaaa aatagtagga
     7561 cgttaagatg aaaaagtcag tatttagatt tcttttagct tcttttagta aaataattct
     7621 tatttgctag aaaggtggag agacatgcgc tggctttttc gtttgatagg ggctttcttt
     7681 tcttttgtgt ggcgtttgtt ttggcgtctg gtttggatag ttgtgctctt atgtgtgctt
     7741 gctttcggac ttctctggta tctgaacggg gattttcaag gagcgctaaa gcaagcagaa
     7801 cggtcagtaa aaattggtca acaaagtatt gaccaatggg agaaaacagg gcaactgcct
     7861 aagttaagcc agacagatag tcaccagcat tctgaaggaa ggtgggcaca ggcctctgct
     7921 cgtatttacc tggatccgca gatggattca cgctttcaag aggcttattt agaagcaatc
     7981 cagaactgga atcaaactgg tgcttttaac tttgaactcg tgactgagtc tagtaaggcg
     8041 gatattacgg ctacggagat gaacgacgga ggcactcctg tggcaggaga ggcggaaagt
     8101 caaactaatc tcttagcagg gcaattcttg tccgtaacgg tgcggttgaa tcattattat
     8161 ttgtccaatc cacactatgg ctactcctat gaacgccttg tccatacggc agaacatgag
     8221 ttaggtcatg cgattggctt ggaccataca gatgagaagt ctgtcatgca accagcaggt
     8281 tccttttatg gtatccagga agaggatgtt gcaaacctcc gaaaaatata tgagactagt
     8341 gagtagggta ctatctttcc ctactttttt tgctataatg gaactatgaa caacttgatt
     8401 aaatcaaaac tagagctctt gccgaccagc cctggttgct acattcataa ggataaaaat
     8461 ggctccatta tctatgtagg aaaggctaaa aatctgcgta atcgagtacg gtcctatttt
     8521 cgtggaagtc atgataccaa gacagaggct ctggtgtctg aaattgtgga ttttgaattt
     8581 attgttacgg agtctaatat tgaggcactt ctcctagaaa tcaacctgat caaggaaaac
     8641 aagcccaagt acaatatcat gctcaaggat gacaagtcct atcctttcat caaaatcacc
     8701 aatgagcgct atccacgctt gattatcact cgtcaggtca aaaaggacgg aggtctttat
     8761 tttggaccct atcccgatgt gggggcagcc aatgaaatca agcggttgct ggatcggata
     8821 ttcccttttc gtaagtgtac caacccgccc tctaaggtct gtttttatta ccatatcggc
     8881 cagtgtatgg ctcacaccat ctgtaagaag gatgaggctt atttcaagtc tatggcccag
     8941 gaggtttctg attttctgaa agggcaggat aacaaaatca ttgatgagct aaagggcaaa
     9001 atggcagcgg cagcccagac tatggagttt gaacgtgcgg cggaataccg tgacctgatt
     9061 caggctattg gaacgcttcg aaccaagcaa cgggtcatgg ctaaggatct ccaaaatcgc
     9121 gatgtctttg gctactatgt ggataagggc tggatgtgtg tgcaggtttt ctttgttcgt
     9181 cagggcaagc tcatcgagcg cgatgtcaat ctcttcccct acttcaatga tccagatgag
     9241 gatttcttga cctatgtggg acaattctat caagaaaaat ctcacctagt tcccaatgag
     9301 gtactgattc cgcaggatat tgacgaagaa gctgtcaagg ctttggtgga taccaagatt
     9361 ctcaaacccc aacgtggaga gaaaaaacag ctggtcaatc tagccataaa aaatgcacgt
     9421 gttagtctgg agcagaaatt taacctgcta gaaaaatcag tcgaaaagac ccaaggagct
     9481 attgaaaatc taggacgttt gctccaaatt ccgactccag ttcgcatcga gtccttcgat
     9541 aactccaaca tcatggggac cagtcctgtt tcagccatgg tggtctttgt taacggcagg
     9601 ccaagtaaaa aagattaccg taagtataaa atcaagaccg tagtcgggcc agacgactat
     9661 gccagtatgc gagaggtcat tcgcagacgc tatggtcgag tacagcgtga ggctttgact
     9721 cctccagatt tgattgtgat tgatgggggg caaggtcaag tcaatattgc taagcaagtc
     9781 atccaagaag aactgggctt ggatattcca attgcaggtc tgcaaaagaa tgataagcac
     9841 caaacccatg aattgctctt tggagatccg ctggaagtgg tggacttgtc tcgcaattct
     9901 caggaatttt tcctcctcca acgcattcaa gatgaggtcc accgctttgc cattaccttc
     9961 caccgccaac tgcgctccaa aaattctttc tcatctcaat tggatgggat tgatggtctg
    10021 ggacctaaac gcaagcaaaa tctcatgagg cacttcaagt ctctgaccaa aatcaaggaa
    10081 gccagtgtgg atgagattgt cgaagttgga gtaccaagag cagtagcaga ggctgtgcaa
    10141 accaagttga acccgcagga aacagaaatc ttgcttcaag tggcggaaga aagagtagat
    10201 taccaaacgg aaggaaacca caataaacca taaaatcgca attttatcag atgttcatgg
    10261 caatgcgacg gcgctagaag cagtgattgc agatgctaaa actcaaggag ccagtgaata
    10321 ttggctcatg ggagacattt tcctccctgg tccaggtgca aatgacttgg tcgctctgtt
    10381 aaaggaactt cctatcacgg caagtgttcg aggcaattgg gatgattgtg tccttgaggc
    10441 tttagatggg caatatggct tagaagaccc acaggaagtt cagctcttgc gtatgacaca
    10501 gtatttgatg gagcgaatgg atcctgcaac gattgtctgg ctacgaagct tgcctttgct
    10561 ggaaaagaaa gaaattgacg gattgcgctt ttctatctct cataatttac ctgacaaaaa
    10621 ctatggtggt gacttgctag ttgagaatga tacagagaaa tttgaccaac tgctagatgc
    10681 ggaaacggac gtggcagttt atggtcatgt tcacaagtag ttgcttcgtt atggtagtca
    10741 agggcaacaa atcatcaatc cagggtcgat tggcatgccc tattttaatt gggaggcgtt
    10801 aaaaaatcac cgtgcccagt atgccgtgat agaggttgaa gatggggaat tggtaaatat
    10861 cctatttcgt aaagtcgctt atgattacga ggctgagtta gaatttgcca aatccaaggg
    10921 ttttcccttt atcgaaatgt atgaagaact acgtcgtgaa gataactatc agaggcacaa
    10981 tctggaacta ttagcaagct taatagaaaa gcataggtat gtagaggatg tgaagaattt
    11041 ttttgatttt ttgtaagagt ttcctaaaat aaccaatgca aactaaaaaa gcgattggct
    11101 gatcccatcg cttttaatat atcttatact caatatgaaa atcaaagagc aaactaggaa
    11161 gctagccgta ggttgctcaa agcacagctt tgaggttgca gataaagctg acgtggtttg
    11221 aagagatttt cgaagagtgt tattgtaact gagattgatc tgggaggtaa gaaccaccta
    11281 gataggtatt gctgagtttt tcaagggttc cgtcttgata gagttctttg agcgctttat
    11341 caaattgctc tttaaactct ttttggtcgc ttgagaaaat gatataattg ctggggctat
    11401 ctgcagaagg taaatcaacg actgagaggt ctaaaccacg gtccttgata atcttttgaa
    11461 cggatacctt gtcaaaaact aggaaatcaa actctccgtt agcaaggtct aggattcgtt
    11521 taccaatatc ctcaccagaa aaattaattg tagcgggatt atcagtgtgt ttctgattcc
    11581 agttattgat gaattgagcg ttagaagttc cggtatcctc ttgtgttgtt ttaccagcga
    11641 tctggtcaag agaagtcaaa ggatttttct tgttgctgac aaggacgagg ggattgttgg
    11701 aaatcggaag cgagtaaagg tatttttcag cacgctcttt tgtgtaactc aaattattgg
    11761 ccgcagcctg atagtgacca gaatcaagtc ctgggaagat actttcccag gcggttcttt
    11821 gaaattgaat ctcgtagtcg ctgagttttt catctactgc ctttagaact tcgatatcga
    11881 agcctgtcag attgccttta tcttcgtagt caaatggtgg cacgtcgcca gctgtagcaa
    11941 ggacgattgt cttttgagcg ctagtctctt tgggtgtagc ttgattccca caggcaacca
    12001 aaaatggtag gatagctagt aataggctaa attttttcat actgtctcca ttcaaatgta
    12061 aagtacttgc tagtttatca gaaactttcc tgataaacca atatatatct tttatgtctg
    12121 tgatagaaaa acttaatcat gaaaaatccc tgcaagctct ttcaaattat ggtagaatgg
    12181 aagcagtaga attagaaaag gaaatcgatt atgaaatttc ttgaattaaa taaaaaacgt
    12241 catgcgacca agcatttcac tgataagcct gttgatccaa aagatgtgcg tacggctatc
    12301 gaaattgcaa ccttggcgcc aagcgcccac aacagccagc cttggaaatt tgtagttgtt
    12361 cgtgagaaaa atgctgaact ggcaaagttg gcttatggtt caaactttga gcaggtatca
    12421 tcagcacctg taaccattgc cctatttaca gatacagacc ttgccaaacg cgctcgtaag
    12481 attgcccgag ttggtggtgc taacaatttc tcagaagaac aacttcaata cttcatgaaa
    12541 aatttgccag ctgaatttgc acgctacaat gaacaacaag tcagcgacta cctagctctc
    12601 aatgcaggtt tggttgccat gaacttggtt cttgcattga cagaccaagg aattggttct
    12661 aacattattc ttggttttga caaatcaaaa gttaatgaag ttttggaaat cgaagaccgt
    12721 ttccgcccag aactcttgat cacagtgggt tatacagacg aaaaattgga accaagctac
    12781 cgcttgccag tagatgaaat catcgagaaa agatagaaag aagaaaaaat gacagcaatt
    12841 gattttacag cagaagtaga aaaacgcaaa gaagacctct tggctgactt gtttagcctt
    12901 ttggaaatca attcagaacg tgatgacagc aaggctgatg cccagcatcc atttgggcct
    12961 ggtccagtaa aagccttgga gaaattcctt gaaatcgcag accgcgatgg ctacccaact
    13021 aagaatgttg ataactatgc aggacatttt gagtttggtg atggagaaga agttctcgga
    13081 atctttgccc atatggatgt ggtgcctgct ggtagcggtt gggacacaga cccttacaca
    13141 ccaactatca aagatggtcg cctttatgcg cgcggggctt cggacgataa gggtcctaca
    13201 acagcttgtt actatggttt gaaaatcatc aaagaattgg gtcttccaac ttctaagaaa
    13261 gttcgcttca tcgttggaac agacgaagaa tcaggctggg cagacatgga ctactacttt
    13321 gagcacgtag gacttgccaa accagatttc ggtttctcac cagatgctga atttccaatc
    13381 atcaatggtg aaaaaggaaa tatcacggaa tacctccact ttgcaggaga aaatacaggt
    13441 gttgcccgtc ttcacagctt tacaggtggt ttacgtgaaa atatggtacc agaatcagca
    13501 acagcagtcg tttcaggtga cttggctgac ttgcaagcta aactagatgc ctttgttgca
    13561 gaacacaaac ttagaggaga actccaagaa gaagctggca aatacaaggt gacgatcatt
    13621 ggtaaatcag cccacggtgc tatgcctgct tcaggtgtca atggcgcaac ttaccttgcc
    13681 ctcttcctca gtcagtttgg ctttgctggt ccagccaaag actaccttga catcgcaggt
    13741 aaaattctct tgaacgatca tgagggtgaa aatcttaaga ttgctcatgt ggatgaaaag
    13801 atgggtgctc tttctatgaa tgccggcgtc ttccgcttcg atgaaacaag tgctgataat
    13861 gccattgccc tcaacatccg ctatccaaaa ggaacaagtc cagaacaaat caagtcaatc
    13921 cttgaaaact tgccagttgt ttctgttagc ctgtctgaac acggtcacac gcctcactat
    13981 gtgccaatgg aagatccact tgtgcaaacc ttgttgaata tctatgaaaa acaaactggc
    14041 tttaaaggtc atgaacaagt catcggtggt ggaacctttg gtcgcttgtt agagcgcgga
    14101 gttgcctatg gtgctatgtt cccagactca attgatacca tgcaccaagc caatgaattt
    14161 atcgccttgg acgatctctt ccgagcagca gcaatctatg ccgaagctat ttacgaattg
    14221 atcaaataaa acgatagaag tctgagatct tatgcttgga cttctttttg gagggaaagt
    14281 agatgtctca aatcgaaaga atcaaacagg ctatcatggc ggattcacag aatgccagct
    14341 atacagagcg tggcattgag cctctctttg cagcgccaaa aactgctcgc atcaatatca
    14401 tcggtcaggc tccgggactt aaaactcaag aagcaggcct ttactggaaa gataaaagtg
    14461 gtgaccgctt gcgggactgg ctaggtgtgg atgaagatac cttttacaat tcaggttatt
    14521 ttgctgtttt gcctatggat ttctactttc caggacatgg caagtcgggt gatcttccgc
    14581 ctcgtacagg ttttgcagaa aaatggcatc cgcagctctt acaggaattg cctgatattc
    14641 agttaaccct cttgattggg caatatgccc aagcctacta tttacaggag aaaatcagtg
    14701 ggaaggtaac ggagagggtg aaacactata aagactatct gccagcctat tttccgctag
    14761 ttcacccatc accacgaaat caaatctgga tggccaaaaa tccttggttt gaggcagaag
    14821 tagtgccaga tttgaaaaaa agaattaaaa ccattttata gtcaatgaaa atcaaagagc
    14881 aaactaggaa gctagtcgta ggctgctcaa agtacagctt tgaggttgca gataaaactg
    14941 acgaagtcgg taacatacgc acggtaaggc gacgctgacg tggtttgaag agattttcga
    15001 agagtattag aagaaaaaga atgaaagaaa tagcctttga cgcattttac cagctttacc
    15061 aaaacgacca gctttctcta gtggatgtga gagaagtgga tgagtttgca gctcttcatt
    15121 tagaatgtgc ccacaaccta ccgcttagtc aattggctga tagttatgat taattggaca
    15181 aagatcgctt gcattatatt atttgcaaat ctggaatgag atcggcgcgt gcttgccaat
    15241 tcctgttaga acaaggttat aatgttatca atgtccaggg tggcatgtta gcctttgaag
    15301 aactttaaaa ttttgcattt ctcctgcttg gtgtggactg ggtaggagag ttttattttt
    15361 agataattct tatttttaag aaaattgaaa acatttaata tttgcctcgt gatgcttttt
    15421 tcagactcct aatcgtggta tactaggtca gtattttata aatatgaagg agatttttat
    15481 ggctaaaaaa ggtaccctaa caggtttgct cctgtttgga atattttttg gtgcggggaa
    15541 cttgattttt ccgccttctc taggtgctct atctggagaa cattttcttc ctgccatcgc
    15601 aggttttgtc ttttcaggcg ttggtatcgc cgtcttgacc cttattattg gaacgctaaa
    15661 tcctaaagga tatatctacg agatttcaac gaagatagcg ccttggtttg cgactcttta
    15721 cctctcagtt ctttacttgt caatcggtcc attctttgct accccacgta ctgctacaac
    15781 agcttacgaa gtagggatta gccccctttt gtcggatgca aataaaggac ttggcttgat
    15841 tgtatttacg gttctgtatt ttgcggcagc ctatttgatt tcgcttaatc catcaaaaat
    15901 cttagaccgc attggacgtg ttttaacgcc agtctttgca attttgattg ttatcttggt
    15961 cgttctggga gctatcaaat atggtggaac aagtcctcaa gctgcttcag ctgcttatca
    16021 agcttctgcc tttggtacag gtttcctaga aggttacaat accttggacg cccttgcttc
    16081 agtggccttt agcgtaatcg cagttcaaac cttgaaacaa cttggatttt caagtaagaa
    16141 agaatacatt tcaactattt gggttgttgg tatcgttgtt gcccttgcct tcagcgctct
    16201 ttacatcggt ttaggttttc ttggaaatca tttcccagta ccagctgaag cgatgaaggg
    16261 tggaacacca ggtgtttaca tcttgtcaca agccactcaa gaaatctttg gctcaacagc
    16321 tcaactcttc cttgcagcta tggttaccgt aacctgcttc acaacgactg ttggtttgat
    16381 tgtgtcaaca gctgagttct ttaatgagcg cttcccacaa atcagctaca aggtttatgc
    16441 gacagccttt accttgattg gatttgctat tgccaatttg ggtcttgatg cgattatcaa
    16501 gtactcaatt ccagtactgg ttatcttgta cccaatcacg attgctatcg ttatgattgt
    16561 cattgtcaac aaatttgttg ccctttcaaa accaggtatg cagttgacaa ttgctgtggt
    16621 tacagttatt gccattgcaa gcgtactagg aagctcgttt aaggttgagt ttcttgcaaa
    16681 tcttgttagc gttcttcctt ttgccaaggc atctctccca tggttggtgc cagccattgt
    16741 tggaatcttg ctctcattgg ttctaccaaa caagcaagaa agcgatgttt ttgaaatgga
    16801 ataatcactt aaatcacttt tgtagccaag tctacaggag tgattttctt tttttatccg
    16861 atgataaatg tgttataata ggtagcgaaa gaggtgaaga aatgaatcaa acagtagaat
    16921 atatcaaaga actgacagcc atcgcatcgc caacgggctt cactcgtgaa atttcgaatt
    16981 atttagtcaa gactctagaa ggttttggtt accagccagt tcgcacagct aagggtggtg
    17041 tcaatgtgac catcaaaggt caaaatgatg agcaacatcg ctatgtgact gcccatgtgg
    17101 atacgctggg tgctattgtt cgtgctgtca aaccagatgg tcgtcttaaa ttggaccgta
    17161 tcggtggttt tccttggaac atgattgaag gtgaaaactg taccgttcat gtggctagca
    17221 caggtcaaaa ggtatcagga accatcctca tccaccaaac ttcttgccat gtctacaagg
    17281 atgcaggaac tgcagaacgc acgcaggaca atatggaagt gcgtttggat gtaaaagtaa
    17341 gcaatgaaaa agaaactcgt gctcttggga ttgaggtcgg tgattttatc agcttcgata
    17401 cacgaactgt tgtgactgat acaggtttta tcaagtctcg tcatttggat gataaggtca
    17461 gcgcagcgat tttgctcaac ctgcttcgta tttataagaa agagaagatt gaattgccag
    17521 taacaactca ttttgctttt tcagtttttg aagaagtagg acatggtgct aattctaaca
    17581 ttcctgctca ggtagtagaa tatctggctg tggatatggg agctatggga gatgaccagc
    17641 agacagatga gtacacagta tctatctgtg tcaaggatgc ttcaggtcct tatcactatg
    17701 acttccgtca acacttggtt gccttggcta aagagcaaga tattccattt aagctcgaca
    17761 tctatccatt ttacggatcg gacgcttcag cagcgatgtc agcaggtgca gaggtcaagc
    17821 atgcacttct tggagctggt attgagtcca gtcactctta tgagcgcacc cacatcgatt
    17881 cagtaatagc gactgagcgt atggttgatg cttatcttaa gagcgaggcg gtatcataga
    17941 atgtgcttaa tctgtcagag aatcgaactc attaaagctg ggcaaaatcc ctactttgta
    18001 aaagaactag aaacaggcta tgttgttatt ggtgactacc aatactttaa gggctatacc
    18061 ttatttttag caaaagatca tgtcacagaa ctccatcata tggaaacttc tgtaaaactt
    18121 cgctttttag aggaaatgag tttagtccaa gaagcagtcg ccaaagcatt tgaggctgaa
    18181 aagatgaata ttgaactcct aggaaatggc gatgcccacg ctcattggca tctttttccc
    18241 cgaagagcag gtgatatgaa gagtcacggt ttgaatggcc gtggcccagt ctggtgggtg
    18301 ccctgggaag aaatggcggc agaagattgc caagtacaat cgccagagct ggaagagatg
    18361 attaaaatct tatctcatga attggagaag tatttggcct aagagagagg aagaaagaaa
    18421 tgaaaaaaag atatgtcatt ttatctggtt tactagcatt gacccttgca gcttgttcgc
    18481 aagaaaaaac caaagttgaa gaaaacactc aaaaaacgga gcaaagcagt caaccggaag
    18541 gaactgtagg tagcaaatct caagcttcta gtcagaaaaa ggcagaagtt agcaataaag
    18601 ggagctatta tagtatccaa gggaaatacg atgagattat cctagctaat aaacgttatc
    18661 ctttgtcaaa agattacaat ccaggagaaa atccaacagc caaggcagag ttgctcaaac
    18721 taatcgcagc tatgcaggct gagggctatc caatcagtga ccaatacagt gggtttagga
    18781 gttatgaaac tcaggctaag ctctaccaag actatgtcaa tcaagatggt aaggaagcag
    18841 cagatcgcta ctcagcacgc ccaggttaca gtgaacacca gacgggtctt gcttttgatt
    18901 tgattgggac agatggggat ttagttactg aagaaaaggc agcccagtgg ctcttggacc
    18961 atgcggctga ctatggcttt gttgttcgct atcttaaagg caaggaaaag gagactggct
    19021 acatggcaga agaatggcat ctccgctacg ttggaaaaga agccaaagaa attgctgcaa
    19081 gtggtctcag tttggaagag tactatggct ttgaaggcgg cgattatgtc gattaaaaat
    19141 gaaagttttc tcttgcattt ttagataaat agtgtataat ggaaaggtat gtgtaaagca
    19201 tacttgtggg aggtaaaaat ctctaattac cgccaaaacc acaaaggagg atttaaaaat
    19261 ggctaaaaaa gtcgaaaaac ttgtaaaatt gcaaatccct gctggtaaag ctacaccagc
    19321 tccaccggtt ggacctgctc ttggtcaagc tggtatcaac atcatgggat tcacaaaaga
    19381 gttcaacgct cgtacagctg accaagctgg tatgatcatt ccagttgtta tctcagttta
    19441 cgaagataaa tcatttactt tcgttacgaa aacaccacca gctgctgttc ttttgaaaaa
    19501 agctgcaggt gttgaaaaag gatcaggtac acctaataaa actaaagttg ctacagttac
    19561 tcgtgcacaa gtacaagaaa ttgcagaaac taagatgcca gatttgaacg cagcaaacgt
    19621 agagtctgca atgcgtatga tcgaaggtac tgctcgttct atgggattca ctgttgttga
    19681 ctaatcaata acacagtaga aaatctcaca gcagtctatt agttgctttt catactaggc
    19741 aagtgactga ggcttgtact tggatacagc aagggagctt aaggccgtag gaagagaaaa
    19801 atagtagact gaaaacccgc aagacttcat catttcgaga agtgacgtgg gagatgaaaa
    19861 tcgattgaac cacttacaag gagaatagaa aatggctaaa aaaagcaaac aacttcgtgc
    19921 tgctcttgag aaaatcgaca gcacaaaagc atacagtgta gaagaagctg tagcacttgc
    19981 aaaagaaact aactttgcaa aatttgatgc aactgtagaa gttgcttaca acttgaacat
    20041 cgacgttaaa aaagctgacc aacaaatccg tggagcaatg gtattgccaa acggtactgg
    20101 taaaacttca cgtgttcttg ttttcgcacg tggtgcaaaa gctgaagaag caaaagctgc
    20161 tggtgcagac tttgttggtg aagatgacct tgttgctaaa atcaacgacg gttggttgga
    20221 cttcgacgta gttatcgcta cacctgatat gatggctctt gttggacgtc ttggacgtgt
    20281 ccttggacca cgtaacttga tgccaaaccc taaaactggt actgtaacaa tggatgttgc
    20341 taaagcagtt gaagagtcta aaggtggtaa aatcacttac cgtgctgacc gtgcaggtaa
    20401 cgttcaagca atcatcggta aagtatcatt tgaagctgaa aaattggttg aaaactttaa
    20461 agctttcaac gaaacaatcc aaaaagtaaa accagctaca gctaaaggaa cttacgtaac
    20521 aaacttgact atcacaacta ctcaaggtgt tggtatcaaa gttgacgtaa actcacttta
    20581 atcagtagtt aaagtaatgt aaaaaagttg aagacactat gtctcaactt tttttgatgt
    20641 acgacgggca tgtcgtatat ctgaggtgta agtcctcggt gggcacctac taccggtgaa
    20701 cccaatagcg attcccaagt ctgacgctgg aaataagaat cgtcagagga agggatagcg
    20761 aaatcgtggc tctacgaaca ggaacgtgat aataaggcct atatagcgga taaggtatct
    20821 ggctggggcg accactatca attataataa aatgaaataa gaataggacg aatcgttcag
    20881 gacagtcaaa tcgatttcta acaat
//
LOCUS       DAVLVF010000027        20464 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVLVF010000027 DAVLVF010000000
VERSION     DAVLVF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20464
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.027"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(106..255)
                     /locus_tag="VYN66_000994"
                     /pseudo
     CDS             complement(106..255)
                     /locus_tag="VYN66_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(409..2040)
                     /gene="rlmD"
                     /locus_tag="VYN66_000995"
     CDS             complement(409..2040)
                     /gene="rlmD"
                     /locus_tag="VYN66_000995"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1495043.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLDMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2114..3088)
                     /locus_tag="VYN66_000996"
     CDS             complement(2114..3088)
                     /locus_tag="VYN66_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV1495044.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3162..3776)
                     /gene="pmp23"
                     /locus_tag="VYN66_000997"
     CDS             complement(3162..3776)
                     /gene="pmp23"
                     /locus_tag="VYN66_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV1495045.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVQLNLYIIKCFTLFSTSG"
     gene            complement(3776..4750)
                     /locus_tag="VYN66_000998"
     CDS             complement(3776..4750)
                     /locus_tag="VYN66_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV1495046.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDVSRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4758..6014)
                     /locus_tag="VYN66_000999"
     CDS             complement(4758..6014)
                     /locus_tag="VYN66_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV1495047.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFLLYED"
     gene            complement(6076..6507)
                     /locus_tag="VYN66_001000"
     CDS             complement(6076..6507)
                     /locus_tag="VYN66_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1495048.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(6509..7111)
                     /locus_tag="VYN66_001001"
     CDS             complement(6509..7111)
                     /locus_tag="VYN66_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV1495049.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7095..7934)
                     /gene="prmC"
                     /locus_tag="VYN66_001002"
     CDS             complement(7095..7934)
                     /gene="prmC"
                     /locus_tag="VYN66_001002"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV1495050.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(7934..9013)
                     /gene="prfA"
                     /locus_tag="VYN66_001003"
     CDS             complement(7934..9013)
                     /gene="prfA"
                     /locus_tag="VYN66_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV1495051.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9010..9600)
                     /locus_tag="VYN66_001004"
     CDS             complement(9010..9600)
                     /locus_tag="VYN66_001004"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV1495052.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            9723..9905
                     /locus_tag="VYN66_001005"
     CDS             9723..9905
                     /locus_tag="VYN66_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV1495053.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10041..11414
                     /gene="mnmE"
                     /locus_tag="VYN66_001006"
     CDS             10041..11414
                     /gene="mnmE"
                     /locus_tag="VYN66_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV1495054.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIVQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEQLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(11461..12396)
                     /gene="dapA"
                     /locus_tag="VYN66_001007"
     CDS             complement(11461..12396)
                     /gene="dapA"
                     /locus_tag="VYN66_001007"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV1495055.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12452..13528)
                     /locus_tag="VYN66_001008"
     CDS             complement(12452..13528)
                     /locus_tag="VYN66_001008"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV1495056.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14159..14231)
                     /locus_tag="VYN66_001009"
     tRNA            complement(14159..14231)
                     /locus_tag="VYN66_001009"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14196..14198),aa:Thr,seq:ggt)
     gene            complement(14331..14840)
                     /locus_tag="VYN66_001010"
     CDS             complement(14331..14840)
                     /locus_tag="VYN66_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV1495057.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(14838..14934)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            14963..15394
                     /locus_tag="VYN66_001011"
     CDS             14963..15394
                     /locus_tag="VYN66_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV1495058.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            15476..15853
                     /gene="mscL"
                     /locus_tag="VYN66_001012"
     CDS             15476..15853
                     /gene="mscL"
                     /locus_tag="VYN66_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV1495059.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(15895..16989)
                     /gene="hemH"
                     /locus_tag="VYN66_001013"
     CDS             complement(15895..16989)
                     /gene="hemH"
                     /locus_tag="VYN66_001013"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV1495060.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            17144..18367
                     /gene="pepT"
                     /locus_tag="VYN66_001014"
     CDS             17144..18367
                     /gene="pepT"
                     /locus_tag="VYN66_001014"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV1495061.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKEQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNAELGSYRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(18403..19032)
                     /locus_tag="VYN66_001015"
     CDS             complement(18403..19032)
                     /locus_tag="VYN66_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495062.1"
                     /translation="MSKVNQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIEIASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            18997..19218
                     /locus_tag="VYN66_001016"
     CDS             18997..19218
                     /locus_tag="VYN66_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495063.1"
                     /translation="MDLFQELIYLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(19295..19836)
                     /locus_tag="VYN66_001017"
                     /pseudo
     CDS             complement(19295..19836)
                     /locus_tag="VYN66_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521835.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(19940..20464)
                     /locus_tag="VYN66_001018"
                     /pseudo
     CDS             complement(19940..20464)
                     /locus_tag="VYN66_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         5899 a         4310 c         3862 g         6393 t
ORIGIN      
        1 agcacaccaa ctatttattt tttgagtttt aaactttggt agaagtcttg aactccttat
       61 atttacataa ctatttgcct ctataactgt tatgacacga aaccaacaag caagttcaag
      121 cacctccata tctttattca aatgaggact aagatattca caaactttat cataaacctc
      181 tttatctttt ttcataaaag gggcatacaa cttagcaaat ttatcctaaa attttttgtt
      241 gcctttatca atcatttcaa cgctccttta aatataaaaa tgatcctatc aatctttata
      301 ttgagcctat tttctaattg ataccttgca agaaaggaat aatagctgtt cctataccaa
      361 gtaggaatgt aataaatgca gaaagtcata tactgtcatt cttttctatt aaatcatttt
      421 gaagtgtctc aaagcatcca tgatggcttc ttctttttca ggtgtacact gtggaataat
      481 ttgtttatcc tttttagact tgttgtaatg ttctcgtaat tctattccac atttcttttt
      541 tatctgtgca atatataatg tcgaaacttt taattcaaat ttattccaaa catattcttt
      601 gatttgagca tatgttgctt tgctctccgc acttgtcaaa tccatctcat ccagctcaat
      661 ttcaacactt atgtgcttat cgacatcgag tttggacaaa agtgctaccg tctcgacgtg
      721 atgcgtttga ggaaatagat ccaccggctg gactttcttc aattcatatc ccaactcttg
      781 gtatagttta atatcacgcg ccatggttgc gacattgcag gagatatagg cgatgcgatc
      841 ggctcctgtt tgggcgcttg ctttgataaa gctttctgtc aagcccttgc gtggaggatc
      901 aaccaagata acggttggtt gaataccttc cttgagccat ttcttcatgg cattttcagc
      961 cgtgtcacag acatagtggg cattagtaat cttgttcaaa gaagcattct tctggctatt
     1021 ctctactgct tctggaatca gttcaacacc gtagacttct ttgacatgct tggcgactga
     1081 taaaccaatg gttccaatac cagaataggc atcaataatc acatcatctt tttttaactc
     1141 tgcaaagtca atggctgttt gatagagttt ctccgccatt tcagtattga cttggtaaaa
     1201 ggctgggcca gcgatttgga agtcatttcc caacatctgg tccgtaatat agtcttgacc
     1261 ataaagagtg cgccactcct taccaaaaat cgcattggta ttctggtcgt tgatattttg
     1321 catgacagac acaatctctg ggaactgctt gataacttgt tcaatcaatt ggtcaacacg
     1381 aaaaactttt ggacgagttg tcaccaaaac gaccatgatt tgtcctgaat agtgaccacg
     1441 acgcaccaca agattccgaa tcaatccaga ttgttccttt tcgtcataag gttttaaatc
     1501 aaaacgacgg agcaggtctc gaagagctac tacgacttgg tcaatgacag gatcctggat
     1561 aaagaaatct tcaaggggca tgaggttatg cgaattctta cggaaaaatc ctgtttccaa
     1621 gacaccattc actcgacgaa cgggcacctg cgccttattg cgatacttga ctggatgttc
     1681 catatcaagc gtttcagcaa cttctacatc tgcaattcca gcaatcttgt agagactgtc
     1741 cttgacttgc ttggttttaa acttgagctg ttctggataa gaaaggtgtc ctaaatccgc
     1801 gattcctgaa cgcaggtaag ccaaatctag atcttgatta cggtgtggtg actggacaag
     1861 gtatttttca acttttccaa agccaatctt tttattgacc ttgaggacac gcatgagaat
     1921 tttttcactc ggtaaagcat tctctacaaa aaagaccaaa ccatctacct tggcaactcc
     1981 tgccccttca tgggtcaaat caacaatttc aacttctaca atatcatttt tctttaacat
     2041 tcttttctct ttctattggc cgacaaaaaa gacggcaacg ttacggttac catccattat
     2101 accatgaaat ttcttaagaa ccaaaactct tgaagaagtt gacaagggct tgccagaggg
     2161 agcttaagaa gttaccaccg tcctctagcg ccttatccgc atcaaagtta aggttgatat
     2221 ttttaaaact gtcgccagct tgtgatacga tgctttgttt aaggtcattt agggttttag
     2281 tgaaatctgc attgctgagg atatcactct ttgagagatt caaggcaaaa ttgatgatga
     2341 tattgatctg gtttcctgtt atgacctgat caagtttgta attttttaag gtatcttcaa
     2401 caatcttgcg tacatcttct tctgtcagat ttcccttgct ttctttagct ttggcgagtc
     2461 ctgacttgat atcagctagg gcaacgttta atttattagc atcatagcct gatttgtcct
     2521 tgttttcagc attgatatct gacaaagcct ttagctcttc ttgagccaaa tctttattgg
     2581 cttgcggcac cttggctcca ttagcttcta gcgaatagta gatcccagcc aaagcactct
     2641 cacctgtaac tggaataggg gctgctacag tgattttggc atgttccata cccagcgtta
     2701 ctgctgcgtt tcggtacata tcctgagtca ccttagtgat attttctggt gtttcaatct
     2761 tgacctcaag tggcgatttg tcacctagct tttgaatctt ggctgatgaa tacaactgta
     2821 agctagagtc attggccaca ttcatgattt tagaataaac atcaggtgtc atggtcttga
     2881 gttctttggt atctgttgag gcattgtagc ccagtttttt aagagtttga tttttttggt
     2941 cttcagatag ggaggaacct aggacatatt caggttggac ataggtttca tcgataactt
     3001 tttgaacatc tgttgctgca tggacgctat tcatagctgt tactgcccac aagaccgcag
     3061 cactagtcag aaagagtttc tttctcatag ggaatttcct cctttacctt tctagagtaa
     3121 tatatctatc ttaaagaaaa cttataacaa aaacacctga actagccaga tgttgaaaag
     3181 agagtgaaac atttgatgat gtaaaggttg agttgcacct gtctagaata ataatagttt
     3241 cctccattta catagagttc ggcaccgtga aaaatggaaa tgggatgaat ataactataa
     3301 gtctttccag tcgtattacc aagcaagggg gcaacagtct cacgagagta ctgtttggct
     3361 agagccaagg tattttcctt gccattttgg gcgataaaat cgatataggc aggtccaaaa
     3421 ttataggctt gaacagctgt ccagatatct acacccttct tctgcgccag atagagattg
     3481 cctgtcagag tttgaatgcc ttgccgaatg ctagaggcat tatcattgat ggtgttggtg
     3541 gaaccacttg cagactcact agactgcata acatcgcctt cttttccttt tgtttcagta
     3601 taaatcatag caagcacaag ctcttcgttt gctggggtgt ctttttcact caatatttct
     3661 cgcaccatgg gttgataggt catgacttgt ttgacatctt gatgaacgcg gtaagcttta
     3721 tagccagcaa aaaggaagac tgctagtaca agcactcttc gaattcgttt aaacattatt
     3781 tactttggat atcctcgata tttttgatta agatagagta ggttccattt tcgttttgga
     3841 taaactcaac agactcggcg tcttgataga cgttattggg aacgatgagc tcaattccat
     3901 ttgataagga gagtttttgg ttttcaaatt tctttaattg gcgactgaca tcaatttcat
     3961 caaattgaac aggttctggt acggcttctt tgacttggtc aataaagctc aaacgagccg
     4021 tcagattgtt gtcaaaaagg tcattagcca atttctcagg tgacaattca ttgctttctt
     4081 ctaggttgtt gaaaatagct gatttgacct tggattgaaa ttgaaaatca tctgtgttaa
     4141 aagattcagc aattctctgg gctgtttttt ccagttcctt gatagatttt ttaggagaaa
     4201 tcttaggagc gacagcaaga agattatctg aaaaatagtt caaaaaagtc ccgttgtact
     4261 tgattcgttt ttcaatcagg tgatacttgc tactctgaag attgaccacc aaggcctcat
     4321 cagctcctgt tccaaatcca ggcaggttat tctgagttag cttgattgga ttatcaactt
     4381 ctcctccgag gtgggtcaag gtctcccgca gggcaattcg caagaaagcg aaatgttcta
     4441 caccttcttt agaaaattga acaaaaatca agtcattggt cttgagattt tcagaaatgc
     4501 taaactcctc tttccagaga ttagccagcg ttactgatgt ctccaacaaa tcgtctgtaa
     4561 tatgattgaa gaagggattt tcttcttcga aaatcccagt cttggcttca tctgaataca
     4621 catgttcaat ttttttacgc aggtattctt cgatttttgg agtaatattg agaaacttat
     4681 ctgctaagaa cagttcggta tcatccggac tgaactggtg aataatggct ttcttaatat
     4741 aaatgtccat aaaagtttta gtcctcgtat agtaggaagg catctgtcaa ttctttgact
     4801 gcacttctca cttcttctaa tacagcctca ttttctgaat tcttaagggt tttaatgatg
     4861 agttcagcca ctttgcgact ttcttcttca ccaaatccac gtgcagtaat ggctgctgct
     4921 ccgatacgaa tcccacttgt cttgaatggt gacaagcttt cgtaagggat tgagttttta
     4981 tttaaggtaa tattgacttc atccaacaag ttttgagcaa ctttgccgtt ttctacaact
     5041 ttagtcacat caacaaggaa gagatggttt tcagttccac ctgaaataat acggaaatca
     5101 gggtcttgca agaagacatc tgccatagcc ttgctgttct taattacatt ggcagcatat
     5161 tccttgaagg ctggatccaa aacttctttg aaggaaactg ccttagccgc cacaacatgc
     5221 tctaaaggac cgccctgaat acctgggaaa atagctgaat tgattttttt agcaagttct
     5281 tcgtcattgg tcaaaatcaa accaccacga ggtccacgaa gggttttgtg ggtcgttgtt
     5341 gttgtgatat gagcgtatgg aactgggctt ggatgaaggc cagccgcaac caaaccagcg
     5401 atatgggcca tgtccaccat gagcttcgca ccgacagcat ctgcgatttc acggaatttt
     5461 gaaaaatcga taatttgaga atatgctgaa gcaccagcta caatcagttt tggttttact
     5521 tcttgggctt gtttcaagat agcatcaaag tctaagagtt ccgtttcagg atcaacacta
     5581 taagaaacaa agttgtaggt ttgaccagag aagctaacag gagccccatg agtcaaatga
     5641 ccacctgatg ccaaatccat tcccataacc gtatcacctg gctcaatcaa ggacatgtaa
     5701 gccgcacagt tagcttggct tcctgaatgt ggttggacat tggcaaattt agcaccgaaa
     5761 atttcttttg cgcgttcaat agcaagagtc tctacaacgt ctactacatc agttccacca
     5821 taataacggc gtcctgggta accctcggca tatttatttg tcaagataga cccttgagct
     5881 gccataacag ccttggaaac tacgttttcc gaagcaatta actcgatatt attttgttgg
     5941 cgttcttctt ctttggcaat agcattccag agatcagcat catatgcttt aaaatcatct
     6001 ttgtcaaaaa tcataggtct tctcctttat tgtgtgacta gtccattagt ttgattttac
     6061 aataagaaaa tcaaactaaa agatgcgaat aaaccgtttc tgcattttat cacaagtata
     6121 gccaactttt tcataaaatg catgagcacc cagacgatga ttggcagaat ttaagcggat
     6181 aaacccataa ccacatcttt ttgcttcttc ttccaaccct tgtagtaaac ttttaccaat
     6241 accttgacct tgcgcttgag gtgaaactgc taaagctaag atattaaatc ctgctttgga
     6301 atagagtgat tcgtaaactt cagcgtggac atatccaagt aagacatgat tagctgcatc
     6361 ctcatagcca agtaggaaat gatgggaatc ctgagacagt ctagctagtt ggctagccgt
     6421 ttcctctgga ctaaaagtat aacccaaagc ctcttggttg atgtcacata tcgctttcac
     6481 atctgtttct tgcaaatctc ttagcatttc aagcctcctc aaaagaaatc tctggcaacc
     6541 gagcaagaat atcttctcgt ttaattgcgc cttggcgtaa gattttcacc ttgtctccag
     6601 acaaatccac aatagttgaa tcctgtccag ttagaaaagc atcgtcttcc agacccagaa
     6661 cctcttggtc aaaatccttc agaatttgtt caaaggttac accacttgcc tgacctgaga
     6721 tattggcaga cggcccaatc aagggacctg tctctcgaat taaatccagt gtgatagggt
     6781 gactgggcat ccgaaatcca atagttgcaa ggtcagaatt tacccaatag ggaactcggt
     6841 cattggcttc gagaataatg gtcaagggac ctggcaaaaa ggtctctaca agtttttgta
     6901 gataagttgg ctgattctta gaaaagtgca agatatcctc gaaagaggcg atattgagat
     6961 tgagtgcctt atctctagga cgacgtttga gttggtaaac atggtcaact gctttttcat
     7021 ctaaggcctt ggcaaaaaga ccataaacag tctctgtagg tagaacgaca gctccaccat
     7081 tttccaactc ttgtctaatc ctgtccatca tcaaccacaa ccatcctatt ttgaccaaat
     7141 tggtctttga gtgttcgaac ttttttttca ggaagatgtt ttctaaaaag ttcgggaaca
     7201 ctttgacctt gcttgtatcc aatttcaagg taaatcttac caccatcctt gagatagtct
     7261 tttgcatctt ccgcaattct acagtaaata gctaggccat cctcgtctgc aaagagagct
     7321 agatgaggct ccgaatgcaa aacattcaaa cctacctctg actcatcttc acgagagata
     7381 tagggtggat tggatacaat tatatcatat ttttcagaaa tttctgcaaa acaatcagat
     7441 tttttaaaaa atatattaag attttgatcg ctagcattct ctgatgcaag ctctaaggcc
     7501 tcttgagaaa catctgctgc tgtcactgac caatctggtc tattttttgc tagagcgaga
     7561 gcaatagctc cactacctgt tccaatatcc agaactgaga gattcgtctc aagattttca
     7621 gttagaataa gctccactaa ctcctctgtt tctggacgag gaatcaaaac ccgttcatcc
     7681 acctttaaat gcattccata aaaatctgcc tgtccaatga tgtactgagc tggcttgtga
     7741 gctgctagtt gctggtaaat atcttctaca aattgttttt cttcctctgt tgtcacctcc
     7801 tgctggaggg caaaaataaa gtctgtaaaa gatagatttt tcagactacg atagacaaaa
     7861 gagaggcttt ccgcttcctc tccttgtctt atcaactctt cttcaaaatt tgaaaataat
     7921 tgagctaatt tcattatttg tttaattctt ctagtttttg tgtttggtca taaagcacca
     7981 aggcatccac aacttcgtcc aatttaccag acaaaatcgt atctagtttt tggagggtca
     8041 agccgatacg gtggtctgtg acacggtttt gtgggaagtt ataagttcgg atccgttctg
     8101 aacggtcacc agtaccgatt gtcgacttac gctcagcgtc ttgttcatcc tgagcaatct
     8161 gagcaaagtg gtcagcgacg cgtgcacgga taatcttcat agccttctcg cggttcttct
     8221 gctgggtacg ttcttcctgc atctcaacct tgatattggt tggcaagtga acgatacgaa
     8281 cggcagtcgc aaccttattg acgttctgtc caccagcacc agaggcgtga tagatgtcga
     8341 cacgaaggtc ttttggatca atgtcgtatt caacctcttc aacttctggc ataacaagaa
     8401 ctgtcgctgt cgaagtatga acacggcctt ggctttctgt cacaggaaca cgttgcacac
     8461 ggtgggcacc tgattcatac ttaagcttag agtatacaga ctgacctgaa accatagcaa
     8521 ccacttcttt aaaaccaccg acaccattca tagaggcttc catgacttca aagcgccaac
     8581 cttgggcttc cgcatacttt tggtacatag ttagcaaatc tccagcgaaa agtgccgctt
     8641 cgtctccacc agctgctcca cggatttcaa ggatgatatt cttgtcatcg tttggatcct
     8701 ttggaaggag caaaattttc agtttttctt catattcttc tttttcagcc ttggcatctt
     8761 tgagttcttg cttggccatt tcttccaagt ccgcatctcc gcctgattcc ttaatcatct
     8821 cttcggcatc gacgatattt tgaaggactt gtttatactc acggtaggct attacggtgt
     8881 cacgattgga agcttcttct tttgaaagct ccataaaacg cttggtgtct gaaacgacat
     8941 cagggtcact cagcaattct cctagttctt cataacggtc ttctacagct tgtagttgat
     9001 catagatgtt catttttctt cattctcctt attgatttca ggggcaaaat aatgtttacg
     9061 gcaaaccgag atataggttt cattaccacc aatctggatc tgttctccat cgtaaacggg
     9121 cagtccatcc tgtgttcgca acaccatggt cgcctttttc ttgcaatact gacagatggt
     9181 cttgatttcg tcaatcttgt ctgctaaaag caagagatat ttggaacctt cgaacaattc
     9241 attgcgaaag tcatttttca agccaaaagc catgacgggt atgtctaact cgtccacaac
     9301 acgagctagg tcataaacat ggtggcgttt gagaaactgg gcttcatcga ccaaaacaca
     9361 gtaaggtttt tctggtaggt ctcggatata gccaaagata tccgttgttt cctcaatcgc
     9421 aagggcaggg cgtttcatgc caattcgact cgacacatag ccaacaccgt cacgcgtatc
     9481 cagagccgag gtcataatca caacaccttt tccttgctcc tcgtagttat aggccacttt
     9541 gagaatctca atcgttttac cagagttcat ggtcccataa cgatagtaca actgtgccat
     9601 gtttcttgct tcacgtccat ttctaaattt ttgctacatt ctagtatatc ataattttct
     9661 taagctttaa acggcaaaat gtggtaaaat ggaagaaatc aaaactagtg gaggaagcta
     9721 ttatgccatt tgtacgcatc gatttatttg aaggacgcac gctcgagcaa aagaaagctc
     9781 ttgctaagga agtaacggaa gcggttgttc gcaacactgg agcacctcaa tctgctgttc
     9841 atgtcatcat caacgacatg ccagaaggaa cttatttccc acaaggggaa atgcgcacca
     9901 aataagctag cttaagcaga attgcttagg ctttttcaat ctccaagtag cacccattga
     9961 agaaatagtc taaatttgtt acaatttgaa aagagacttg gaaaattcca agaaaagagc
    10021 aattaattaa aggaaacatt atgattacac gtgaatttga taccatcgct gctatctcta
    10081 ctccactagg tgaaggggct attggtattg tccgcctgag cggaacagac agttttgcta
    10141 ttgtgcaaaa gatttttaaa ggaaaagact tgaacaaggt tgccagccac actctcaact
    10201 acggtcacat tattgatcct ctgactggta aagtcatgga cgaggttatg gttggggcta
    10261 tgaagtctcc aaagaccttc actcgtgagg atattatcga gattaacacc cacggtggga
    10321 ttgcggtgac caatgaaatt ctccagctag ctattcgtga aggggctcgg ttggcagaac
    10381 ctggtgaatt taccaaacgt gcttttttaa acggtcgcgt agacttgaca caggcagagg
    10441 ctgtgatgga tatcatccgt gccaagactg acaaggccat gaacattgcg gtcaaacaat
    10501 tagacggctc cctttctgac ctcattaaca atacccgtca agaaatcctc aatacacttg
    10561 cccaagttga ggtcaatatc gactatcctg aatatgatga tgttgaggaa gctactactg
    10621 ccgttgtccg tgagaagact atggagtttg agcaattgct aaccaatctt cttagaacag
    10681 cccgtcgcgg caaaatcctt cgtgaaggaa tttcaacagc tatcatcgga cgtcccaacg
    10741 ttgggaaatc cagccttctc aacaacctct tgcgtgagga caaggctatc gtaacagata
    10801 tcgctgggac aacacgagat gtcatcgaag agtacgtcaa catcaatggt gtacctctca
    10861 aattgattga tacagccggt attcgtgaaa cagatgacat agtggaacaa atcggtgttg
    10921 agcgttcgaa aaaagccctc aaggaagccg acttggttct actagtgcta aatgccagtg
    10981 aacaactgac tgcgcaagac agacaacttc ttgaaattag ccaagatacc aatcgcatta
    11041 ttctacttaa taaaaccgac ctgccagaaa cgattgaaac ttcgaaacta cctgaagacg
    11101 ttatccgtat ttcagtcctt aaaaaccaaa acatcgacaa gattgaagag cgaatcaaca
    11161 acctcttctt tgaaaatgct ggcttggtcg agcaagatgc tacttacttg tcaaacgccc
    11221 gtcacatttc cctgattgaa aaagcagttg aaagcctaca agccgttaat caaggtcttg
    11281 agctggggat gccagttgat ttgcttcaag ttgacttgac tcgtacttgg gaaatcctcg
    11341 gagaaatcac tggggatgct gctccagatg aactcatcac ccaactcttt agccaattct
    11401 gtttaggaaa ataagaaaaa tccatgatcc ttcattcggt catggatttt attgtcttta
    11461 ttagtaatct ggtcttaaga cccctgttac agttgcctta gttgcttcgt agtcgccatc
    11521 tacgacaacc ttgataatgc gtttggcatc ttcttctggt gctggaacaa gaggtagacg
    11581 agtgggtcca gcttcaaatc ccatatagtt aagaattgcc ttaactggag caggacttgg
    11641 ataagagaag agagcattaa ccttaggaat gaatttacgc tgaattgctg cggctttctt
    11701 catatcgctt tctgcaatgg cagtaaacat ctcgtgcatt tcatccccat ttgtatgaga
    11761 ggcaacagaa ataaccccat ccgccccaag gttcatggca tggaaagcat ctccatcctc
    11821 acctgtataa atcaagaact cttcaggctt gtgctcaatc aagtaagcca tattagccaa
    11881 gctagtacat tctttgacac cgataatatt tggatggtca gccaagcgaa gcatggtttc
    11941 tggagtcaat tcgacaacta cacgccctgg aatgttatag ataataattg gtaggtcaga
    12001 agcatctgca atagccttaa agtgctgata catcccttct tgagaaggtt tgttgtagta
    12061 aggaacaata gcaagcccag ctgcgaaacc accaaattcc gctacttctt tgacaaactc
    12121 aatagagtca cgcgtatcat tagtacctac acccgcaatc aaaggaacgc gtccattgac
    12181 aaccttttgt acagccgcaa acaactccaa ctcctcatcg tgggtcaaag ttggactctc
    12241 agcagtcgtt cctgcgagaa gaattccatc cgtatgatgg gccaataaat gctcaatcaa
    12301 ggctggaata gcatcaaagt taatggaacc atcctcatgg aagggggtaa taaaggctgt
    12361 aatgatttta cattctttta aatcttgata agacacgaaa acacctctct atttcaaaga
    12421 aggagttcat ctgaactcct aaacgatatg actattttaa ttcaaatttc aattcagctg
    12481 ttggacgaac caatccacgt tcatgaagag tttcagcaat ctgaactgag ttccaagcag
    12541 cacctttgag aaggttatct gaaacaaccc acatgtgaat tcctttttct gcatccaagt
    12601 ctttacggat acgaccaaca aaggtatcac gtgaaccaac tgcattgatg gcttgaggat
    12661 agatttgatg aactacatca tcttcaagaa cagcacctgg gaaggatgcg atagctgctt
    12721 tgacttcttc aattggagcc acttcctttg tttcaatata aactgactca gagtgagctg
    12781 acaagactgg aatacgcaca catgtcgcag atactgcaat gctatcatct tccataattt
    12841 tcttagtttc cttggtcatc ttcatctctt cgtacgtgta atcattatca gtgaagacat
    12901 caatttgtgg aagagcgtta aaggcgatag gataatgttt cttgtcacca cctgaaggca
    12961 agatttccgc atgcaaatca cgtggtttca ctccatcatt caagacctca cgaagttcac
    13021 gttgtgtctc aagaattgct cccataccag cacctgaaac ggcttgataa gttgaaacaa
    13081 tgatacggtc caagccccat ttttggcgaa ccggctcaag agccaccatc atttggattg
    13141 ttgaacagtt agggcaggca atgattccgt tgtgggcatc aagtgcatga gcattgacct
    13201 ctggaacaac caaaggaaca tctggatttt gacggaaata agatgtatta tccactacta
    13261 ccacgccagc ttttactgcg tatggtgcat acttagctga tgtcgaacca cctgctgaaa
    13321 agagagcaat atcaactcct tcaaaagctg tttcagtcgt ttcttcaatc gtaatatctt
    13381 gatctttaaa tttcaatgac ttacctgctg aacgtgcaga agcaaggtaa cggattttat
    13441 cgattggaag tgttgattct tccaacattt ttatcatctg agcaccgaca gcacctgtcg
    13501 cgccgactac agcaactgta tatcccataa ataacctctt tcggaatttt ctaaaaattt
    13561 ctataataga agtattatac tactttttcc atgaattgca aagctttttc atcatttttt
    13621 ggaaaataaa aatccccaat cgctagacta gggattaaga ggcatcttca tgtgatgagc
    13681 aggttcacac aactcatcaa ggtccgctcc tgcgttatga cctccttatg ctcaatagta
    13741 gcccgaagac tacctatcga ctcatcgcat ctactattct actagataga gtcatttttg
    13801 tcaacagcca aaactcttta tactcttcga aaatctcttc aaacttcgtc agcgtcgcct
    13861 tgccgtatgt atggttactg acttcgtcag tttcatctac aacctcaaaa acatgttttg
    13921 agctgacttc gtcagtttca tctacaacct caaaaacatg ttttaagctg acttcgtcag
    13981 ttctatctac aacctcaaag cagtgctttg agcaacctgc ggctagtttc ctagtttact
    14041 ctttgatttt cattgagtat tagttttatt tacacaggtg cataagaaat atattggaaa
    14101 aattgggggc agacataaca aaaacccttg taaatcaagg gcttttctgt ctatttaatg
    14161 ctaattgccg ggattgaacc ggcgacctca tccttaccat ggatgcgctc tgccaactga
    14221 gctaaatcag cttacttaag aagtatacta tattcagaaa aacttgtcaa gtttccttag
    14281 aaattttcca taggaaaaag ccaggtaaga actacctagc ttatcttctt ctatttactt
    14341 aaatcaattc gacgaccaat tttaccaata ataatcgctc caataatcaa tgaagcaaat
    14401 tcaccgattc ctgttgtaaa ccaagtaagg aagaatggta attctgctac aatatttaac
    14461 tctgcagcga tagtaaacat ggaaattgaa aagaggattg aaaagaagaa atgatctttt
    14521 cgaatcaatc cgttaaagag gtaatccttg ctatattttg caaaaagcca aacacctaaa
    14581 ctgaggaata ctaaggttga accaccacca acaaagacat ctagcagtcc gaagctaaag
    14641 aaattagcaa tcatacaacc gatggtaact ccgatgatgt acttaggatt gtaaaaagcc
    14701 ataaagttca tcatttctga aatacgaaac tgataagcac catagctgat ggcatttagg
    14761 ggcggtgtga cagtcaaaac gacgtagata gcagcgacga ttgcaatatc tgcaacatca
    14821 cgaatagtta attttttcat cttttctcct ttggcggtta tccgcgtgta atatgcttgg
    14881 tgaaagaagc taagcaccaa gggttgattc attcaacctt actagtatag cacaatagcc
    14941 tgctttatgc tatacttaaa gcatgaaaaa acctattcaa aaatttttta acaatgaaat
    15001 cttagcctat ctcttttttg gccttgcgac aactctagtt tcgattctct cacgactagt
    15061 catttatcaa ctaagtcatc aggaactcct tgctacggcc ttggcaaata ttatcggtat
    15121 cttatttgcc tttatcacaa atgatacgat tgtatttaag caaaaaaaga aaaatagact
    15181 tatccgttta gtgaaatttt ctcttgctcg cctttctact tttctgttag acttgttttt
    15241 aacttttctc tttgtaaccc aatttcctca catcataggg caattcgtaa ctgaaaacat
    15301 tgataagata aatgctattg aaacaataat tgcacaaata ttcattatta tcatcaatta
    15361 tatcttgagc aaggtctata tttttagaaa ataatgctcc atttatgagc cttttaattg
    15421 ccttctataa tgatttagga tacaatgaaa cacgaaacat tgaaaggaaa acagtatgtt
    15481 aaaaaatcta aaatcgttct tgcttcgagg aaatgttatt gaccttgctg tcggtgttgt
    15541 cattgcctct gcttttggtg ctatcgttac ttcacttgta aacgacatta tcactcctct
    15601 tattttaaat cccgctttga aagctgctaa agtcgaacgt atcgctcaac tttcttggca
    15661 tggagtcggc tatggtaact ttttaagtgc tattatcaat tttatctttg tgggtaccgc
    15721 cctcttcttt attatcaagg gcattgaaaa agcacagaaa ctgactggca taaagaaaga
    15781 aaaaactgcc gaaaaaaaac caaccgaatt ggaagtcctt caagaaataa aagctctcct
    15841 tgagaaaaaa taatcgatta aaaggatcta gcgcaaagct aaatcctttt ttctttactc
    15901 tttgggtaac ttgcctgctt tttcaagcat ttttttgata agataaggca tcttaacatt
    15961 ctcccgccct tttttctcaa tcagtttttt cacaaattct ggcatttgta aatcttcaga
    16021 ttcagctaaa atgtttttcg tctcgtctga aggaactaac tcatcaaagg tctcttcttc
    16081 tgggagaaat tccttaaact cttgattttc attgactcta actgtattga ccaaagcatc
    16141 aatcaaacga gaatctgtat ttggcattgg tggacgatgg tagtttaccc caaattcctg
    16201 gcataagtca taacattcta catcattgtc aaacaagacc tcaatatgct cactgatgaa
    16261 actaataggt acaaaaatat aatggtctgg atgttctgtc tgttctctga gatactccaa
    16321 aacatctggt ttaatccatg gaataccaat atcactctca ctctgccaag tgttggtata
    16381 ttgttctgaa ctcaagccca gtttttcagc gactagcttg ctattttcga aaatttggtc
    16441 tatataagga tcaccaaagt ccaaggcaaa aatgggcaca ctatgggctg aaaagatgac
    16501 tttaaagctg tcttgcttta cttcttcttt taaaatctta gcaatttcat ctgcccaata
    16561 gtttaagagc gcttcttcct gataccagtc cttaatgacc aaaaattgaa tttgtttgct
    16621 ttctaaaaat ttttcatagc ccatgacaga gtagaaagaa taatggggct ccaaaatcaa
    16681 gcaaatacac tgttcaattc cgtctgcttc catttgaccg atcacatctg gaataaaggg
    16741 actggaaaac ttattagcaa agtagacact gtattcattc cccagcctag cttctaccaa
    16801 ggcaacctct tgacgggtaa ttttttgcag aggcgtgcct ccaattcgta cataattatc
    16861 atagagagtc tgaatctcgt ggtcttgggg tctcacccca cgacgaatat ttgtgaaaaa
    16921 atcagctaca ccttcaaagg taatctcttc tggtgaaccg aaggtcatca ttaaaattgc
    16981 ttttttcatg actgcttcct tgtgatgttt ttatcaagtt tattttatca ttaattaatc
    17041 gataagtaaa cgctaatata gggacattct caatcttctg tcttttgttt ttctcttctt
    17101 tctatgatac aatggaaaaa ataaattcaa aaggagtttt tttatgactt atccaaatct
    17161 cttggaccgc ttcttaacct acgttaaggt caacacgcgc tctgatgaac actctactac
    17221 tactccaagt acacagagtc aggttgactt cgcaacaaat gtcctaattc ctgaaatgaa
    17281 acgtgttgga ctgcaaaatg tttactatct accgaatggt tttgctattg gaaccttgcc
    17341 agccaacgat ccgtctttaa cacgtaagat tggttttata tcgcacatgg atactgctga
    17401 ttttaatgct gaaggagtca atccacaggt aattgaaaac tacgatggtg gtgtgattga
    17461 actagggaat tctggtttca aactcgatcc agctgacttc aagagtcttg aaaaatatcc
    17521 aggacaaacg ctcatcacaa cagatggaac aagcttgcta ggtgctgatg acaagtcagg
    17581 aattgctgaa attatgacag ccattgaata tctaactgct catcctgaaa ttaagcactg
    17641 tgagattcgt gttggttttg gtccagatga agaaatcggt gttggtgcca ataaatttga
    17701 tgcagaagat tttgatgtgg attttgccta cactgttgat ggtggtccac taggtgaact
    17761 tcagtacgag actttctcag ccgctggtgc tgaattgcat ttccaaggcc gtaatgtcca
    17821 ccctggtact gccaaagagc agatggtcaa tgcccttcag ctagcaattg attttcataa
    17881 tcaacttcca gaaaatgacc gacctgagtt aactgaaggt taccaaggtt tttaccatct
    17941 aatggatgtg acaggtagtg ttgaggaggt gcgtgcaagc tacatcattc gtgattttga
    18001 aaaagatgcc tttgaagcgc gtaaagcatc catgcaatct atcgctgata agatgaatgc
    18061 agaacttggg agctaccgtg tcactctcaa cttgacagac cagtactaca atatgaaaga
    18121 agtcattgaa aaagatatga ctccaattac cattgctaaa gccgttatgg aagatctagg
    18181 tatcacgcct attatcgaac caatccgggg tggaacagac ggctctaaga tttcctttat
    18241 gggaatccca actccgaata tctttgcagg tggcgaaaat atgcacggac gttttgaata
    18301 cgttagcctt cagactatgg aacgtgcagt tgataccatc attggcatcg tagcttataa
    18361 aggctaaaaa gacgaggtag ctcagctact tcgcctttct ttttattcta ctggtttttc
    18421 ttgatttcca gtagttgtag aagattctgt tgtttcattt tctgaagttg attcagcagg
    18481 tttagaatct cttgtattgc ttggtttgtt ttcgtcgcta gcaatttcaa tgttagattc
    18541 tgcagttgcg tttggttggt tctcagcact ggtgttatca ctatttgctt cagcatttct
    18601 tgctggactt gtttcttcac ttgcgctagc ttttgactgg atttgatgat tcaaaactag
    18661 aatagctttt gtcgattcaa gtaaagctgt tttgtcttta ctcttagcag aaagttgatc
    18721 taataatgca tccaccttat caaagtccgc atcagatcca ttattacttt ctaaataaga
    18781 gtgaagcgac atgagaatat cgtagagttt ttgatagagt acaagtgtct gagaatcttg
    18841 ctcagcattt tccttttctt gttgaagggc gctagcgata cgagtcaaga catcttttac
    18901 ctgactgttt acttcatcca agtctgcatc agccttgttt gtggcagctt ttagattttc
    18961 tacttcttct gccaaggatt gtctgattcc ttcttcatgg atttgttcca agagttgatt
    19021 taccttgctc aaaagacttt ctacttcttc cttgctatct gtcgcagatt attggttgcc
    19081 atctaccatg tactcctaaa acaggagagt tataatccaa gattacaagg ccttacagaa
    19141 ataagaaatc cagataagac aatgttcgtc caagacgcta ttcgcttcgc acagcagcac
    19201 ggattcaata tgctttaatt ttaaagttta ggtgtcaaga cctcttttta gtgtgcccaa
    19261 aatttagaga agtaatcaat caactaactt ttattttttt caaactttca gtaaactgac
    19321 ctaaagctaa ctcaatctgt ctttgtagat gcttctgcta tcagctagaa gttgatctac
    19381 ttttgccaag actgccttct catcaaaagt tccaggttga tagttggatt gcagggatgg
    19441 aatcttgttt ttcaaagccg cttcatatcc cttagtttga accttgatgt agtgattgtg
    19501 gtcgccatga ggaatcacaa aaccttctga atcttcactt ataattcgat tggcatcaaa
    19561 accatgacca tcttcttcct catgatggac atgtagtgac ggattactta atacagaact
    19621 agaagaactt cctacctctt ccgtgttaga gtgtgatggg ggattgttaa gagatgactt
    19681 aggaatatag tgatagtgat ccccatgtct tactatataa gcatcacctg tatctctgac
    19741 aatatcatta gggttaaaga catatgtggc tgctaattca cctgccgaca agtcactctc
    19801 aggaatgaaa tgatagtgac caccatgtgg tactatagta gattgaaata gaatatgagc
    19861 aaattgataa ggggatttta aagtaatttc taacaatgat ttagaaacta tgatgtgcta
    19921 ttctaaattc aactcactat atataaccat catcggtagt ataacgtccc tgtaattttg
    19981 ctacagatac ttctgcacta gctcctttat cgtctttacc atgttcttgt ttttggcgat
    20041 tgatttcatc ttttgttcgt acattttctg catgagcttg atctttaagg taaacataat
    20101 actttccatc taccttaata atatatcctc ccttaaccta actgacgata tcttgatctt
    20161 tcggctgata gttgggggct ttcattaata gctcttcact aaagagcgca tcaaaaggaa
    20221 ctttaccatt atagtagtga taatgatcgc catgagaagt tacataacct tgatctgtaa
    20281 tcttaataac aatttgtttt gcttgaattc cttctttttg actaacctag tctggagtca
    20341 aattttcagt cttcttagtg tctttattac tgtttacata tgaaacacga tttttatctg
    20401 tattggcctg ttagctatgt tggttcagag cataaacaca cagacttaag gaaaggataa
    20461 caac
//
LOCUS       DAVLVF010000028        18497 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVLVF010000028 DAVLVF010000000
VERSION     DAVLVF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18497
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.028"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..1132
                     /gene="dnaN"
                     /locus_tag="VYN66_001019"
     CDS             <1..1132
                     /gene="dnaN"
                     /locus_tag="VYN66_001019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV1495064.1"
                     /translation="HFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGITLI
                     GSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQI
                     VLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRPIL
                     TGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIE
                     TVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERA
                     RLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLID
                     SLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            1197..1391
                     /locus_tag="VYN66_001020"
     CDS             1197..1391
                     /locus_tag="VYN66_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV1495065.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEVTRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1475..2590
                     /gene="ychF"
                     /locus_tag="VYN66_001021"
     CDS             1475..2590
                     /gene="ychF"
                     /locus_tag="VYN66_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV1495066.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2662..3231
                     /locus_tag="VYN66_001022"
     CDS             2662..3231
                     /locus_tag="VYN66_001022"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV1495067.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSIDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3232..6741
                     /gene="mfd"
                     /locus_tag="VYN66_001023"
     CDS             3232..6741
                     /gene="mfd"
                     /locus_tag="VYN66_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1495068.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIVASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            6799..7065
                     /locus_tag="VYN66_001024"
     CDS             6799..7065
                     /locus_tag="VYN66_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV1495069.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7058..7426
                     /locus_tag="VYN66_001025"
     CDS             7058..7426
                     /locus_tag="VYN66_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV1495070.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7546..8814
                     /locus_tag="VYN66_001026"
     CDS             7546..8814
                     /locus_tag="VYN66_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV1495071.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFSIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8811..10088
                     /gene="tilS"
                     /locus_tag="VYN66_001027"
     CDS             8811..10088
                     /gene="tilS"
                     /locus_tag="VYN66_001027"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV1495072.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10092..10634
                     /gene="hpt"
                     /locus_tag="VYN66_001028"
     CDS             10092..10634
                     /gene="hpt"
                     /locus_tag="VYN66_001028"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV1495073.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10650..12608
                     /gene="ftsH"
                     /locus_tag="VYN66_001029"
     CDS             10650..12608
                     /gene="ftsH"
                     /locus_tag="VYN66_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV1495074.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAHHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            12730..13209
                     /locus_tag="VYN66_001030"
     CDS             12730..13209
                     /locus_tag="VYN66_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1495075.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            13304..13375
                     /locus_tag="VYN66_001031"
     tRNA            13304..13375
                     /locus_tag="VYN66_001031"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13337..13339,aa:Glu,seq:ttc)
     gene            13627..15172
                     /locus_tag="VYN66_001032"
     rRNA            13627..15172
                     /locus_tag="VYN66_001032"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            15224..15296
                     /locus_tag="VYN66_001033"
     tRNA            15224..15296
                     /locus_tag="VYN66_001033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15257..15259,aa:Ala,seq:tgc)
     gene            15421..18322
                     /locus_tag="VYN66_001034"
     rRNA            15421..18322
                     /locus_tag="VYN66_001034"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            18399..>18497
                     /gene="rrf"
                     /locus_tag="VYN66_001035"
     rRNA            18399..>18497
                     /gene="rrf"
                     /locus_tag="VYN66_001035"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6075 a         3042 c         4245 g         5135 t
ORIGIN      
        1 tcatttttca attaataaaa atttatttct acaagcatta aatactacta agagagctat
       61 tagttctaaa aatgccattc ctattttatc aacagtaaaa attgacgtga ccaatgaagg
      121 tattacttta attggttcaa atggtcaaat ttcaattgaa aattttattt ctcaaaaaaa
      181 tgaagatgct ggtttgttaa ttacttcttt aggttcgatc cttcttgaag cttctttctt
      241 tatcaatgta gtatctagtt tacctgatgt gactcttgat tttaaagaaa ttgaacaaaa
      301 tcaaattgtt ttaaccagtg gcaaatcaga aattacccta aaaggaaaag atagcgaaca
      361 atatccacga atccaagaaa tttcagcaag cactccttta atacttgaaa caaaattact
      421 caagaaaatt attaatgaaa cagcctttgc tgcaagtaca caagagagtc gtccgatttt
      481 aacaggtgtc cacttcgtat tgagtcaaca caaagagtta aaaacagttg caacagactc
      541 tcatcgccta agccagaaaa aattgactct tgaaaaaaat agtgatgatt ttgatgtcgt
      601 aattcctagc cgttctctac gcgaattttc agcggtattt acagatgata tcgaaactgt
      661 agagattttc tttgccaata accaaatcct ctttagaagc gaaaatatta gcttctatac
      721 tcgtctccta gaaggaaact atcctgatac agatcgcttg attccaacag actttaacac
      781 tactattact tttaatgtgg taaacttacg ccagtcaatg gagcgtgccc gtcttttatc
      841 aagtgcgact caaaatggta ctgtgaaact tgaaattaag gatggggttg ttagcgccca
      901 tgttcactct ccagaagttg gtaaagtaaa cgaagaaatc gatactgatc aggttactgg
      961 tgaagatttg accattagtt tcaacccaac ttacttgatt gattctctta aagctttaaa
     1021 tagcgaaaag gtgaccatta gctttatctc agctgttcgt ccatttactc ttgtgccagc
     1081 agatactgac gaagacttca tgcagctcat tacaccagtt cgtacaaatt aagtgaaaga
     1141 ggttgagcct ggctcgcctc ttttatgata taatcgaaaa agaaaaggag agtagtatgt
     1201 atcaagttgg aaattttgtt gagatgaaaa aatcacacgc ttgtacaatc aagtcgactg
     1261 gtaaaaaggc taatcgttgg gaagttacac gtgtaggagc agatatcaaa ataaaatgca
     1321 gtaattgtga gcatgttgtc atgatggggc gatatgattt tgagcgaaaa atgaataaaa
     1381 ttattgactg agaaccctta gttagagggt tagcacttta tccctttttg tgttataata
     1441 ttagggattg aaatgaaaac ggagaatgag aaatatggct ttgacagcag gtatcgttgg
     1501 tttgccaaac gttggtaaat caacattatt taatgcaatt acaaaagcag gagcagaggc
     1561 agcaaactac ccatttgcga ctattgatcc aaatgttgga atggtggaag ttccagatga
     1621 acgcctacaa aaactaactg aaatgataac tcctaaaaag acagttccca caacatttga
     1681 atttacagat attgcaggga ttgtaaaagg agcttcaaaa ggagaagggc tagggaataa
     1741 attcttggcc aatattcgtg aagtagatgc gattgttcac gtagttcgtg cttttgatga
     1801 tgaaaatgtg atgcgcgagc aaggacgtga agacgccttt gtagatccac ttgcagatat
     1861 tgatacaatt aatctggaat tgattcttgc tgacttagaa tcagtgaaca aacgatatgc
     1921 gcgtgtagaa aagatggcac gtacgcaaaa agataaagaa tcagtagcag aattcaatgt
     1981 tcttcaaaag attaaaccag tcctagaaga cgggaaatca gctcgtacca ttgaatttac
     2041 agatgaggaa caaaaggttg tcaaaggtct tttccttttg acgactaaac cagttcttta
     2101 tgtagctaat gtggacgagg atgtggtttc agaacctgac tctatcgact atgtcaaaca
     2161 aattcgtgaa tttgcagcga cagaaaatgc tgaagtagtc gttatttctg cgcgtgctga
     2221 ggaagaaatt tctgaattgg atgatgaaga taaaaaagag tttcttgaag ccattggttt
     2281 gacagaatca ggtgtagata agttgacgcg tgcagcttac cacttgcttg gattgggaac
     2341 ttacttcaca gctggtgaaa aagaagttcg cgcttggact ttcaaacgtg gtatgaaggc
     2401 tcctcaagca gctggtatta tccactcaga ctttgaaaaa ggctttattc gtgcagtaac
     2461 catgtcatat gaagatctag tgaaatacgg atctgaaaag gccgtaaaag aagctggacg
     2521 cttgcgtgaa gaaggaaaag aatatatcgt tcaagatggc gatatcatgg aattccgctt
     2581 taatgtctaa aaattaataa atggtgtcaa ttaggttgga aaaaaaattc caaccctttt
     2641 ggcttttgaa aggaaaaata aatgaccaaa ttacttgtag gcttgggaaa tccaggggat
     2701 aaatattttg aaacaaaaca caatgttggt tttatgttga ttgatcaact agcgaagaaa
     2761 cagaatgtca cttttacaca cgataagata tttcaagctg acctagcatc ctttttccta
     2821 aatggagaaa aaatttatct ggttaaacca acgaccttta tgaatgaaag tggaaaagca
     2881 gttcatgctt tattaactta ctatggtttg gatattgacg atttacttat catttacgat
     2941 gatcttgaca tggaagttgg gaaaattcgt ttaagagcaa aaggctcagc aggtggtcat
     3001 aatggtatca agtctattat tcaacatata ggaactcagg tctttaaccg tgttaagatt
     3061 ggaattggaa gacctaaaaa tggtatgtca gttgttcatc atgttttgag taagtttgac
     3121 agggatgatt atatcggtat tttacagtct attgacaaag ttgacgattc tgtaaactac
     3181 tatttacaag agaaaaattt tgagaaaaca atgcagaggt ataacggata aatggtgacc
     3241 ttattagatt tattctcaga aaatgatcag attaaaaaat ggcatcaaaa tttaacagat
     3301 aagaaaagac aactaatact tggtttatca acatctacta aggctcttgc aattgcaagc
     3361 agtttagaaa aagaagatag gattgtgtta ttgacgtcaa cttatggaga agcagaagga
     3421 cttgttagtg atcttatatc tatcttgggt gaggaactcg tctatccatt tttggtagat
     3481 gatgctccta tggtggagtt tttgatgtct tcacaggaaa aaattatttc acgggttgaa
     3541 gccttgcgtt ttttgactga ttcatctaag aaagggattt tagtttgtaa tatcgtagca
     3601 agtcgattga ttttaccgtc tcccaatgca ttcaaagata gtattgtaaa aatctcagtt
     3661 ggtgaagaat atgatcaaca cgcgtttatc catcagttaa aggaaaatgg ctatcgaaaa
     3721 gttactcaag tacaaactca gggcgaattt agtcttcgag gagatatttt agatattttt
     3781 gaaatatccc agttagaacc ttgtcgaatt gagttttttg gtgatgaaat tgatggtatc
     3841 aggtcatttg aagtagaaac acaattatcg aaagaaaata agacagaact cactatcttt
     3901 ccagctagtg atatgctttt gagagaaaag gattatcaac gaggacagtc agctttagaa
     3961 aaacaaattt caaaaacttt atcacctatt ttgaaatcat acctagaaga aattctttca
     4021 agttttcacc aaaaacaaag tcatgcagac tctcggaagt ttttatcttt gtgctatgat
     4081 aagacatgga ctgtctttga ttatattgaa aaagataccc caatattctt tgatgattat
     4141 caaaaattga tgaatcagta tgaagtcttt gaaagagact tagcgcagta ctttacagaa
     4201 gaattacaga atagtaaagc attttctgat atgcagtatt tttctgatat tgaacaaatc
     4261 tataaaaaac aaagtccagt gacctttttc tctaatcttc aaaagggttt aggaaatctc
     4321 aaatttgaca aaatttatca attcaatcaa tatcctatgc aggaattttt caatcagttt
     4381 tcttttctaa aagaagaaat tgaacgatat aaaaaaatgg attacaccat tattctgcag
     4441 tctagcaatt caatgggaag taaaacattg gaggatatgt tagaggaata tcagattaaa
     4501 ttggattcta gagataagac aaatatctgt aaagaatctg taaacttaat agagggtaat
     4561 ctcagacatg gttttcattt tgtagatgaa aagattttat tgataactga acatgagatt
     4621 tttcaaaaga aattaaagcg tcgttttcga agacaacatg tttcaaatgc agagagatta
     4681 aaagattaca atgaacttga aaaaggggac tatgttgtcc atcatatcca tgggattggt
     4741 caatatctag gaattgaaac cattgaaatc aagggaattc atcgcgatta tgtcagtgtc
     4801 caataccaaa atggtgatca aatttctatc cccgtggaac agattcatct actgtccaaa
     4861 tatatttcaa gtgatggtaa agctccaaaa ctcaataaat taaatgacgg tcattttaaa
     4921 aaggccaagc aaaaggttaa gaaccaggta gaggatatag ctgatgattt aatcaaactc
     4981 tactctgaac gtagtcagtt gaagggtttt gctttctcag ctgatgatga tgatcaagat
     5041 gcctttgatg atgctttccc ttatgttgaa acggatgatc aacttcgtag tattgaggaa
     5101 atcaagaggg atatgcaggc ttctcagcca atggatcgac ttttagttgg ggatgttggt
     5161 tttggaaaga ctgaagttgc tatgcgtgca gcctttaaag cagtcaatga tcacaaacag
     5221 gttgtcattc tagttccgac gacggtttta gcgcaacagc actatacgaa ttttaaggaa
     5281 cgattccaaa attttgcagt taatattgat gtgttgagtc gctttagaag taaaaaagag
     5341 cagactgcaa cacttgaaaa attgaaaaac ggtcaagtcg atattttgat tggaacacat
     5401 cgtgttttgt caaaagatgt tgtgtttgct gatttgggct tgatgattat tgatgaggaa
     5461 cagcgatttg gtgtcaagca taaggaaact ttgaaagaac tgaagaaaca agtggatgtc
     5521 ctaaccttga ccgctacgcc aatccctcgt accctccata tgtctatgct gggaatcaga
     5581 gatttatctg ttattgaaac tccgccgact aatcgctatc ctgttcagac ctatgttttg
     5641 gaaaagaatg atagtgtcat tcgtgatgct gtcttgcgtg aaatggagcg tggaggtcaa
     5701 gtttattatc tttacaacaa agttgacaca attgttcaga aggtttcaga attacaggag
     5761 ttgattccgg aggcttcgat tggatatgtt catggtcgaa tgagtgaagt ccagttggaa
     5821 aatactctat tagactttat tgagggacaa tacgatatct tggtgacgac tactattatt
     5881 gagacagggg tggacattcc aaatgctaat actttattta ttgaaaatgc ggaccatatg
     5941 ggcttgtcaa ccttatatca gttaagagga agagtcggtc gtagtaatcg tattgcttat
     6001 gcttatctca tgtatcgtcc agaaaaatca atcagtgaag tctctgaaaa gagattagaa
     6061 gcgattaaag gatttacaga attgggctct ggctttaaga ttgcaatgcg agatctttcg
     6121 attcgtggag caggaaatct tttaggaaaa tcccagtctg gtttcattga ttctgttggt
     6181 tttgaattgt attcgcagtt attagaggaa gctattgcta aacgaaacgg taatgctaac
     6241 gctaacacaa gaaccaaagg gaatgctgag ttgattttgc aaattgatgc ctatcttcct
     6301 gatacttata tttctgatca acgacataag attgaaattt acaagaaaat tcgtcaaatt
     6361 gacaaccgtg tcaattatga agagttacaa gaggagttga tagaccgttt tggagaatac
     6421 ccagatgtag tagcctatct tttagagatt ggtttggtta aatcatactt ggacaaggtc
     6481 tttgttcaac gtgtggaaag aaaagataat aaaattacaa ttcaatttga aaaagtcact
     6541 caacggctgt ttttagctca agattatttt aaagctttat ccgtaacgaa cttaaaagca
     6601 ggcatcgctg agaataaggg attaatggag cttgtatttg atgtccaaaa taagaaagat
     6661 tatgaaattt tagaaggtct gctgattttt ggagaaagtt tattagagat aaaagagttt
     6721 aaggaagaaa attccatttg atatttttct tctataaaat agataaaaat ggtacaataa
     6781 taaattgagg taataaggat gagattagat aaatatttaa aagtatcgcg aattatcaag
     6841 cgtcgtacag tcgcaaagga agtagcagat aaaggtagaa tcaaggttaa tggaatcttg
     6901 gccaaaagtt caacggactt gaaagttaat gaccaagttg aaattcgctt tggcaataag
     6961 ttgctgcttg taaaagtact agagatgaaa gatagtacaa aaaaagaaga tgcagcagga
     7021 atgtatgaaa ttatcagtga aacacgggta gaagaaaatg tctaaaaata ttgtacaatt
     7081 gaataattct tttattcaaa atgaatacca acgtcgtcgc tacctgatga aagaacgaca
     7141 aaaacggaat cgttttatgg gaggggtatt gattttgatt atgctattat ttatcttgcc
     7201 aacttttaat ttagcgcaga gttatcagca attactccaa agacgtcagc aattagcaga
     7261 cttgcaaact cagtatcaaa ctttgagtga tgaaaaggat aaggagacag catttgctac
     7321 caagttgaaa gatgaagatt atgctgctaa atatacacga gcgaagtact attattctaa
     7381 gtcgagggaa aaagtttata cgattcctga cttgcttcaa aggtgataaa atggaaaatt
     7441 tattagacgt aatagagcaa tttttgagtt tgtcagatga aaagctggaa gaattggctg
     7501 ataaaaatca attattgcgt ttacaagaag aaaaggaaag gaagaatgcg taaattctta
     7561 attattttgt tgctaccaag ttttttgacc atttcaaaag tcgttagcac agaaaaagaa
     7621 gtcgtctata cttcgaaaga aatttattac ctttcacaat ctgactttag tatttatttt
     7681 agagaaaaat taagttctcc catggtttat ggagaggttc ctgtttatgc gaatgaagat
     7741 ttagtagtgg aatctgggaa attgactccc aaaacaagtt ttcaaataac cgagtggcgc
     7801 ttaaataaac aaggaattcc agtatttaag ctatcaaatc atcaatttat agctgcggac
     7861 aaacgatttt tatatgatca atcagaggta actccaacaa taaaaaaagc atggttagaa
     7921 tctgacttta aactgtacaa tagtccttat gatttaaaag aagtgaaatc atccttatca
     7981 gcttattcgc aagtatcaat cgacaagacc atgtttgtag aaggaagaga atttctacat
     8041 attgatcagg ctggatgggt agctaaagaa tcaacttctg aagaagataa tcggatgagt
     8101 aaagttcaag aaatgttatc tgaaaaatat cagaaagatt ctttctctat ttatgttaag
     8161 caactgacta ctggaaaaga agctggtatc aatcaagatg aaaagatgta tgcagccagc
     8221 gttttgaaac tctcctatct ctattatacg caagaaaaaa taaatgaggg tctttatcag
     8281 ttagatacga ctgtaaaata cgtatctgca gtcaatgatt ttccaggttc ttataaacca
     8341 gagggaagtg gtagtcttcc taaaaaagaa gataataaag aatattcttt aaaggattta
     8401 attacgaaag tatcaaaaga atctgataat gtagctcata atctattggg atattacatt
     8461 tcaaaccaat ctgatgccac attcaaatcc aagatgtctg ccattatggg agatgattgg
     8521 gatccaaaag aaaaattgat ttcttctaag atggccggga agtttatgga agctatttat
     8581 aatcaaaatg gatttgtgct agagtctttg actaaaacag attttgatag tcagcgaatt
     8641 gccaaaggtg tttctgttaa agtagctcat aaaattggag atgcggatga atttaagcat
     8701 gatacgggtg ttgtctatgc agattctcca tttattcttt ctattttcac taagaattct
     8761 gattatgata cgatttctaa gatagccaag gatgtttatg aggttctaaa atgagggaac
     8821 cagatttttt aaatcatttt ctcaagaagg gatatttcaa aaagcatgct aaggcggttc
     8881 tagctctttc tggtggatta gattccatgt ttctatttaa ggtattgtct acttatcaaa
     8941 aagagttaga gattgaattg attctagctc atgtgaatca taagcagaga attgaatcag
     9001 attgggaaga aaaggaatta aggaagttgg ctgctgaagc agagcttcct atttatatca
     9061 gcaatttttc aggagaattt tcagaagcgc gtgcacgaaa ttttcgttat gatttttttc
     9121 aagaggtcat gaaaaagaca ggtgcgacag ctttagtcac tgcccaccat gctgatgatc
     9181 aggtggaaac gattttgatg cgcttgattc gaggaactcg cttgcgctat ctatcaggaa
     9241 ttaaggagaa gcaagtagtc ggagagatag aaatcattcg tcccttcttg cattttcaga
     9301 aaaaagactt tccatcaatt tttcactttg aagatacatc aaatcaggag aatcattatt
     9361 ttcgaaatcg tattcgaaat tcttacttac cagaattgga aaaagaaaat cctcgattta
     9421 gggatgcaat cttaggcatc ggcaatgaaa ttttagatta tgatttggca atagctgaat
     9481 tatctaacaa tattaatgtg gaaaatttac agcagttatt ttcttactct gagtctacac
     9541 aaagagtttt acttcaaact tatctgaatc gttttccaga tttgaatctt acaaaagctc
     9601 agtttgctga agttcagcag attttaaaat ctaaaagcca gtatcgtcat ccgattaaaa
     9661 atggctatga attgataaaa gagtaccaac agtttcagat ttgtaaaatc agtccgcagg
     9721 ctgatgaaga ggaagatgaa cttgtgttac actatcaaaa tcaggtagct tatcaaggat
     9781 atttattttc ctttggactt ccattagaag gtgaattaat tcaacaaata cctgtttcac
     9841 gtgaaacatc catacacatt cgtcatcgaa aaacaggaga tgttttgatt caaaatgggc
     9901 atagaaaaaa actcagacgt ttatttattg atttgaaaat ccctatggaa aagagaaact
     9961 ctgctcttat tattgagcaa tttggtgaaa ttgtctcaat tttgggaatt gcgaccaata
    10021 atttgagtaa aaaaacgaaa aatgatataa tgaacactgt actttatata gaaaaaatag
    10081 ataggtaaaa aatgttagaa aacgatatta aaaaagtcct cgtttcacac gatgaaatta
    10141 cagaagcagc taaaaaacta ggtgctcaat taactaaaga ctatgcagga aaaaatccaa
    10201 tcttagttgg gattttaaaa ggatctattc cttttatggc tgaattggtc aaacatattg
    10261 atacacatat tgaaatggac ttcatgatgg tttctagcta ccatggtgga acagcaagta
    10321 gtggtgttat caatattaaa caagatgtga ctcaagatat caaaggaaga catgttctat
    10381 ttgtagaaga tatcattgat acaggtcaaa ctttgaagaa tttgcgagat atgtttaaag
    10441 aaagagaagc agcttctgtt aaaattgcaa ccttgttgga taaaccagaa ggacgtgttg
    10501 tagaaattga ggcagactat acctgcttta ctatcccaaa tgagtttgta gtaggttatg
    10561 gtttagacta caaagaaaat tatcgtaatc ttccttatat tggagtattg aaagaggaag
    10621 tgtattcaaa ttagaaagaa taatctttaa tgaaaaaaca aaataatggt ttaattaaaa
    10681 atccttttct atggttatta tttatctttt tccttgtgac aggattccag tatttctatt
    10741 ctgggaataa ctcaggagga agtcagcaaa tcaactatac tgagttggta caagaaatta
    10801 ccgatggtaa tgtaaaagaa ttaacttacc aaccaaatgg tagtgttatc gaagtttctg
    10861 gtgtctataa aaatcctaaa acaagtaaag aaggaacagg tattcagttt ttcacgccat
    10921 ctgttactaa ggtagagaaa tttaccagca ctattcttcc tgcagatact accgtatcag
    10981 aattgcaaaa acttgctact gaccataaag cagaagtaac tgttaagcat gaaagttcaa
    11041 gtggtatatg gattaatcta ctcgtatcca ttgtgccatt tggaattcta ttcttcttcc
    11101 tattctctat gatgggaaat atgggaggag gcaatggccg taatccaatg agttttggac
    11161 gtagtaaggc taaagcagca aataaagaag atattaaagt aagattttca gatgttgctg
    11221 gagctgagga agaaaaacaa gaactagttg aagttgttga gttcttaaaa gatccaaaac
    11281 gattcacaaa acttggagcc cgtattccag caggtgttct tttggaggga cctccgggga
    11341 caggtaagac tttgcttgct aaggcagtcg ctggagaagc aggtgttcca ttctttagta
    11401 tctcaggttc tgactttgta gaaatgtttg tcggagttgg agctagtcgt gttcgctctc
    11461 tttttgagga tgccaaaaaa gcagcaccag ctatcatctt tatcgatgaa attgatgctg
    11521 ttggacgtca acgtggagtc ggtctcggcg gaggtaatga cgaacgtgaa caaaccttga
    11581 accaactttt gattgagatg gatggttttg agggaaatga agggattatc gtcatcgctg
    11641 cgacaaaccg ttcagatgta cttgatcctg cccttttgcg tccaggacgt tttgatagaa
    11701 aagtattggt tggccgtcct gatgttaaag gtcgtgaagc aatcttgaaa gttcacgcta
    11761 agaacaagcc tttagcagaa gatgttgatt tgaaattagt ggctcaacaa actccaggct
    11821 ttgttggtgc tgatttagag aatgtcttga atgaagcagc tttagttgct gctcgtcgca
    11881 ataaatcgat aattgatgct tcagatattg atgaagcaga agatagagtt attgctggac
    11941 cttctaagaa agataagaca gtttcacaaa aagaacgaga attggttgct caccatgagg
    12001 caggacatac cattgttggt ctagtcttgt cgaatgctcg cgttgtccat aaggttacaa
    12061 ttgtaccacg cggccgtgca ggcggataca tgattgcact tcctaaagag gatcaaatgc
    12121 ttctatctaa agaagatatg aaagagcaat tggctggctt aatgggtgga cgtgtagctg
    12181 aagaaattat ctttaatgtc caaactacag gagcttcaaa cgactttgaa caagcgacac
    12241 aaatggcacg tgcaatggtt acagagtacg gtatgagtga aaaacttggc ccagtacaat
    12301 atgaaggaaa ccatgctatg cttggtgcac agagtcctca aaaatcaatt tcagaacaaa
    12361 cagcttatga aattgatgaa gaggttcgtt cattattaaa tgaggcacga aataaagctg
    12421 ctgaaattat tcagtcaaat cgtgaaactc acaagttaat tgcagaagca ttattgaaat
    12481 acgaaacatt ggatagtaca caaattaaag ctctttacga aacaggaaag atgcctgaag
    12541 cagtagaaga ggaatctcat gcactatcct atgatgaagt aaagtcaaaa atgaatgacg
    12601 aaaaataacc ctgagagagg ctggagtctc tcttttttgt gcagtttagg agctaaaggg
    12661 aacagaatgg agaaaatgga acaaatgtgt tttctaatct gttagactgt atctagaaag
    12721 gggaaaatta tgattaaaga attgtatgaa gaagtccaag ggactgtgta taagtgtaga
    12781 aatgaatatt accttcattt atgggaatta tcggattggg accaagaagg catgctctgc
    12841 ttacatgaat tgattagtag agaagaagga ctggtagacg atattccacg tttaaggaaa
    12901 tatttcaaaa ccaagtttcg aaatcgaatt ttagactata tccgtaagca ggaaagtcag
    12961 aagcgtagat acgataaaga accctatgaa gaagtgggtg agatcagtca tcgtataagt
    13021 gaggggggtc tctggctaga tgattattat ctctttcatg aaacactaag agattataga
    13081 aacaaacaaa gtaaagagaa acaagaagaa ctagaacgcg tcttaagcaa tgaacgattt
    13141 cgagggcgtc aaagagtatt aagagactta cgcattgtgt ttaaggagtt tactatccgt
    13201 acccactagt aagtcatgca aaaaaaaatg aaaaaaatta gaaaaagtag ttgacaaagt
    13261 ttgaaaagac tgtataatag taagagttga aaataacaac tcaggtccgt tggtcaaggg
    13321 gttaagacac cgccttttca cggcggtaac acgggttcga atcccgtacg gactatggta
    13381 tgttgcgtca ggagcacttg atgaaaaaaa gtttaaaaaa acttaaaaat cttcaaaaaa
    13441 gtgttgacaa gtgaaagtgg ctgtgatata ctaatatagt tgtcgcttga gagaagcaag
    13501 tgacaaagac ctttgaaaac tgaacaagac gaaccaatgt gcagggcgct acaacataag
    13561 ttgtagtact gaacaatgaa aaaacaataa atctgtcagt gacagaaatg agtaagaact
    13621 caaactttta atgagagttt gatcctggct caggacgaac gctggcggcg tgcctaatac
    13681 atgcaagtag aacgctgaag gaggagcttg cttctctgga tgagttgcga acgggtgagt
    13741 aacgcgtagg taacctgcct ggtagcgggg gataactatt ggaaacgata gctaataccg
    13801 cataagagta gatgttgcat gacatttgct taaaaggtgc acttgcatca ctaccagatg
    13861 gacctgcgtt gtattagcta gttggtgggg taagggctca ccaaggcgac gatacatagc
    13921 cgacctgaga gggtgatcgg ccacactggg actgagacac ggcccagact cctacgggag
    13981 gcagcagtag ggaatcttcg gcaatggacg gaagtctgac cgagcaacgc cgcgtgagtg
    14041 aagaaggttt tcggatcgta aagctctgtt gtaagagaag aacgagtgtg agagtggaaa
    14101 gttcacactg tgacggtatc ttaccagaaa gggacggcta actacgtgcc agcagccgcg
    14161 gtaatacgta ggtcccgagc gttgtccgga tttattgggc gtaaagcgag cgcaggcggt
    14221 tagataagtc tgaagttaaa ggctgtggct taaccatagt aggctttgga aactgtttaa
    14281 cttgagtgca agaggggaga gtggaattcc atgtgtagcg gtgaaatgcg tagatatatg
    14341 gaggaacacc ggtggcgaaa gcggctctct ggcttgtaac tgacgctgag gctcgaaagc
    14401 gtggggagca aacaggatta gataccctgg tagtccacgc tgtaaacgat gagtgctagg
    14461 tgttagaccc tttccggggt ttagtgccgt agctaacgca ttaagcactc cgcctgggga
    14521 gtacgaccgc aaggttgaaa ctcaaaggaa ttgacggggg cccgcacaag cggtggagca
    14581 tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt cttgacatcc ctctgaccgc
    14641 tctagagata gagttttcct tcgggacaga ggtgacaggt ggtgcatggt tgtcgtcagc
    14701 tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccctatt gttagttgcc
    14761 atcatttagt tgggcactct agcgagactg ccggtaataa accggaggaa ggtggggatg
    14821 acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgctacaat ggctggtaca
    14881 acgagtcgca agccggtgac ggcaagctaa tctcttaaag ccagtctcag ttcggattgt
    14941 aggctgcaac tcgcctacat gaagtcggaa tcgctagtaa tcgcggatca gcacgccgcg
    15001 gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccacgagagt ttgtaacacc
    15061 cgaagtcggt gaggtaaccg taaggagcca gccgcctaag gtgggataga tgattggggt
    15121 gaagtcgtaa caaggtagcc gtatcggaag gtgcggctgg atcacctcct ttctaaggat
    15181 aaggaactgc gcattggtct tgtttagtct tgagaggtct tgtggggcct tagctcagct
    15241 gggagagcgc ctgctttgca cgcaggaggt cagcggttcg atcccgctag gctccattgg
    15301 tgagagatca ccaagtaatg cacattgaaa attgaatatc tatatcaaat agtaacaaga
    15361 aaataaaccg aaaacgctgt agtattaata agagtttatg actgaaaggt cagaaaataa
    15421 ggttaagtta ataagggcgc acggtggatg ccttggcact aggagccgac gaaggacgtg
    15481 acaaacgacg atatgccttg ggtagctgta agtaagcgat gatccaggga tttccgaatg
    15541 ggggaaccca acaggtaata cctgttacct acatctgtta aggatgtgag gaggaagacg
    15601 cagtgaactg aaacatctaa gtagctgcag gaagagaaag caaaagcgat tgccttagta
    15661 gcggcgagcg aaacggcaga agggcaaacc gaagagttta ctcttcgggg ttgtaggact
    15721 gcaatgtgga ctcaaagatt atagaagaat gatttgggaa gatcagccaa agagagtaat
    15781 agcctcgtat ttaaaatagt ctttgtactt agcagtatcc tgagtacggc gggacacgtg
    15841 aaatcccgtc ggaatctggg aggaccatct cccaacccta aatactccct agtgaccgat
    15901 agtgaaccag taccgtgagg gaaaggtgaa aagcaccccg ggaggggagt gaaatagaac
    15961 ctgaaaccgt gtgcctacaa caagttcgag cccgttaatg ggtgagagcg tgccttttgt
    16021 agaatgaacc ggcgagttac gttatgatgc gaggttaagt tgaagagacg gagccgtagg
    16081 gaaaccgagt ctgaataggg cgccttagta tcatgacgta gacccgaaac catgtgacct
    16141 acccatgagc aggttgaagg tgcggtaaga cgcactggag gaccgaacca gggcacgttg
    16201 aaaagtgctt ggatgacttg tgggtagcgg agaaattcca aacgaacttg gagatagctg
    16261 gttctctccg aaatagcttt agggctagcg tcgacattag agattcttgg aggtagagca
    16321 ctgtttgggt gaggggtcca tcccggatta ccaatctcag ataaactccg aatgccaatg
    16381 aattatggtc ggcagtcaga ctgcgagtgc taagatccgt agtcgaaagg gaaacagccc
    16441 agaccaccag ctaaggtccc aaaataattg ttaagtggaa aaggatgtgg ggttgcacag
    16501 acaactagga tgttagctta gaagcagcta ttcattcaaa gagtgcgtaa tagctcacta
    16561 gtcgagtgac cctgcgccga aaatgtaccg gggctaaaac aatttaccga agctgtggat
    16621 acctttatag gtatggtagg agagcgttct atgtgtgatg aaggtatacc gtgaggagtg
    16681 ctggaacgca tagaagtgag aatgccggta tgagtagcga aagacaggtg agaatcctgt
    16741 ccaccgtaag actaaggttt ccaggggaag gctcgtccgc cctgggttag tcgggaccta
    16801 aggagagacc gaaaggtgta tccgatggac aacaggttga tattcctgta ctagagtatg
    16861 tagtgatgga gggacgcagt aggctaacta aagcagacga ttggaagagt ctgtctaagc
    16921 agtgaggtgt gaattgagtc aaatgcttaa ttctataaca ttgagctgtg atggggagcg
    16981 aagtttagta gcgaagttag tgacgtcaca ctgccaagaa aagcttctag cgtttaaaca
    17041 tactctaccc gtaccgcaaa ccgacacagg tagtcgaggc gagtagcctc aggtgagcga
    17101 gagaactctc gttaaggaac tcggcaaaat gaccccgtaa cttcgggaga aggggtgctg
    17161 acttaaagtc agccgcagtg aataggccca agcaactgtt tatcaaaaac acagctctct
    17221 gctaaatcgt aagatgatgt atagggggtg acgcctgccc ggtgctggaa ggttaagagg
    17281 agtgcttagc gtaagcgaag gtatgaattg aagccccagt aaacggcggc cgtaactata
    17341 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
    17401 tgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
    17461 gttacccgcg acaggacgga aagaccccat ggagctttac tgcagtttga tattgagtgt
    17521 ctgtaccaca tgtacaggat aggtaggagt ctaagagatc gggacgccag tttcgaagga
    17581 gacgctgttg ggatactacc cttgtgttat ggccactcta acccagatag gtgatcccta
    17641 tcggagacag tgtctgacgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
    17701 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg cagagtgtaa aggtataagg
    17761 gagcttgact gcgagagcta caactcgagc agggacgaaa gtcgggctta gtgatccggt
    17821 ggttccgtat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
    17881 atctccccca agagttcaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
    17941 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
    18001 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt aggaaatttg
    18061 agaggatctg ctcctagtac gagaggacca gagtggactt accgctggtg taccagttgt
    18121 cttgccaaag gcatcgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
    18181 gtgtgaaacc cacctcaaga tgagatttcc catgattata tatcagtaag agccctgaga
    18241 gatgatcagg tagataggtt agaagtggaa gtgtggcgac acatgtagcg gactaatact
    18301 aatagctcga ggacttatcc aaagtaactg agaatatgaa agcgaacggt tttcttaagt
    18361 tgaatagata ttcaattttg agtaggtatt actcagagtt aagtgacgat agcctaggag
    18421 atacacctgt acccatgccg aacacagaag ttaagcccta gaacgccgga agtagttggg
    18481 ggttgccccc tgtgaga
//
LOCUS       DAVLVF010000029        18372 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVLVF010000029 DAVLVF010000000
VERSION     DAVLVF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.029"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(46..855)
                     /locus_tag="VYN66_001036"
     CDS             complement(46..855)
                     /locus_tag="VYN66_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1495076.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(857..1660)
                     /locus_tag="VYN66_001037"
     CDS             complement(857..1660)
                     /locus_tag="VYN66_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1495077.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYELLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(1670..3157)
                     /gene="tacF"
                     /locus_tag="VYN66_001038"
     CDS             complement(1670..3157)
                     /gene="tacF"
                     /locus_tag="VYN66_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV1495078.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIIKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNRIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIILLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            3493..4200
                     /locus_tag="VYN66_001039"
     CDS             3493..4200
                     /locus_tag="VYN66_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV1495079.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            4205..5227
                     /locus_tag="VYN66_001040"
     CDS             4205..5227
                     /locus_tag="VYN66_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1495080.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            5244..6113
                     /gene="licA"
                     /locus_tag="VYN66_001041"
     CDS             5244..6113
                     /gene="licA"
                     /locus_tag="VYN66_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV1495081.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            6103..6981
                     /locus_tag="VYN66_001042"
     CDS             6103..6981
                     /locus_tag="VYN66_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1495082.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            6993..7682
                     /locus_tag="VYN66_001043"
     CDS             6993..7682
                     /locus_tag="VYN66_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV1495083.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYVSGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            7778..8626
                     /gene="dprA"
                     /locus_tag="VYN66_001044"
     CDS             7778..8626
                     /gene="dprA"
                     /locus_tag="VYN66_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV1495084.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            9515..10558
                     /locus_tag="VYN66_001045"
     CDS             9515..10558
                     /locus_tag="VYN66_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV1495085.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            10725..12812
                     /gene="topA"
                     /locus_tag="VYN66_001046"
     CDS             10725..12812
                     /gene="topA"
                     /locus_tag="VYN66_001046"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV1495086.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            12918..13277
                     /locus_tag="VYN66_001047"
     CDS             12918..13277
                     /locus_tag="VYN66_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV1495087.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYILMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            13370..14002
                     /locus_tag="VYN66_001048"
     CDS             13370..14002
                     /locus_tag="VYN66_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV1495088.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            14046..14702
                     /locus_tag="VYN66_001049"
     CDS             14046..14702
                     /locus_tag="VYN66_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV1495089.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            14721..15353
                     /locus_tag="VYN66_001050"
     CDS             14721..15353
                     /locus_tag="VYN66_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV1495090.1"
                     /translation="MNFQLAKYSLLKKFLENIGFTTLEEYGAIFKYLIENVKTDRQII
                     CSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            15381..15815
                     /locus_tag="VYN66_001051"
     CDS             15381..15815
                     /locus_tag="VYN66_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEV1495091.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            15827..16864
                     /gene="leuB"
                     /locus_tag="VYN66_001052"
                     /pseudo
     CDS             15827..16864
                     /gene="leuB"
                     /locus_tag="VYN66_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            16861..17130
                     /locus_tag="VYN66_001053"
     CDS             16861..17130
                     /locus_tag="VYN66_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV1495092.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRTWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            17134..17493
                     /gene="leuD"
                     /locus_tag="VYN66_001054"
     CDS             17134..17493
                     /gene="leuD"
                     /locus_tag="VYN66_001054"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV1495093.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            17655..18278
                     /locus_tag="VYN66_001055"
                     /pseudo
     CDS             17655..18278
                     /locus_tag="VYN66_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         5747 a         3174 c         3891 g         5560 t
ORIGIN      
        1 tttttgaata acctctatct tagtagtttg tataatcccc ctcaatcagt ttttgcgata
       61 agctttaatg ctatgactat accactcttg catttctttt gatggtggtg tcatataatc
      121 gccatacatc tgggttaaaa attggtcata ttttttggga acaggcaaca tacggccctc
      181 aaactcagtt aaaatcagtt ctttaaaggt atcaactggg aagatttctt tcatcccttc
      241 cttaccgatc ccaactcctc cttcatattg aggaatgttg gttacagcat ttttgactag
      301 ttgatcaatt ttcttgtaaa agtagcgagg attgacaaat cggagagcgt accagctaca
      361 taatctaaga aaatctttta gtttgctatc accgtgaact gctcgtgatt ttttgatata
      421 agctagttga cgaagagcca catacttata gctcttgtcg acaatgctca agtctgtaaa
      481 tcgatcaatt gggaagacat cgatgaaaag gctggtatca tgacgcttgt acttaacatg
      541 gtcttctata acagtagaag tgtccaaaat cgatgcgaaa ttatggaagt accaagaaga
      601 tgtatcgtag gaaagaacct tgtagcgagg gtgattttct tcttcaataa tcttcagtaa
      661 acgctcataa tcctcacgat aaagggaaat atcaatatca tcatcccaag gaatcatacc
      721 tttgtggcgg atggctccaa gcatggttcc ataactgaga aaataaggaa tatcatgttt
      781 cttacaagtc tcatcaatat agtccagcag ggctagttga atttctttaa tttctttttt
      841 ttctaaatat tgcatcctaa tcctccaatt tataagcgtg aaattcatga ctgtagaagc
      901 gtttttcttc tggtggtagg gtcatataat ctccataaaa ttgtgtcaaa atagtatcaa
      961 atttttcagg tgcaggaagg cttaaattct caaagggtaa atcgattgtt ttatcaaagg
     1021 taccacttgg gaagacttcc ttttccttaa attttgaagg gataaaagcc atatactgcc
     1081 cattttcacg actatatttt tgaatttctt tctcgatttt atttgcaaaa taacgaggag
     1141 aaactggtcg aaggagcaac caaaaggctg ttcgtatcca atcttttaaa aggctatcct
     1201 tatagacaat atttttatgt ttactgaaag acagcagttt gaagctttcc agtttataac
     1261 aagtatcaat gaccttagga tcatcaaagc gatccatagg gaaaatatcg ataaagatac
     1321 cagactcata ggtttttgta tttcgagtat caataatttt agtcgtactg tcggttatct
     1381 tgataaagtt gttaaagtag ttcttatcag tttctaagga taggagttca tacttgcttt
     1441 tttccttttg aaaaatgtta ataaatcgtt ggtagtcttc tctaggcatg gataaatcaa
     1501 tatcgtcgtc ccaagggata aagccctcat gtcgaaccgc cccaatcaga gtaccgtagt
     1561 taataatata gttgatattg tgctttttac agagagtatc aatataatcc aaaatttcta
     1621 attcaatttg tttggcatct tcaacggtta gttgtttcat tttaaactcc tatgattttt
     1681 taaatttatt ttttaaggct agaacatggt ttaaaaattc atagaaaatg ctatcttttg
     1741 tgaagacaag tagactaata taagagatag ctgatagcaa gataatcaaa ccagtattaa
     1801 tcaaaaatgg caaattaatg accatatcca caggatacac gaaattaatc aggaaataaa
     1861 ttgctacaaa ggaaagtgaa aagagagagt atcgaacagt atagctaaag atatgtccca
     1921 agtggatgag ttgttttcta tggatgaaaa tgatatagaa aacaagtaga gaagcctctg
     1981 ataacatagt tgtcagtaag tagtattcgg gagccacgat acggttgaaa aagaggagac
     2041 tattcaagcc caaattgagt agcccagcaa agactgtata aactgtgata cgtttttcat
     2101 agccattggt aaagagaatt tgggaaccaa gaatggtatc taaggccagg ataatcgtac
     2161 gaaaagcgaa gagagaggtc aagatgccgc ctccgatata tttttcacta ccgtaaagta
     2221 ggatggcatt tggtcctaaa accatgagtc caaaactcag tggaatgata aagaagttaa
     2281 agattcgact acctctatta accagagaaa cataggcttc tttgtctcct ttccccagat
     2341 agtaactgag acgaggcaca ctcactccaa ttgcacctgt tacaacccca gctataacgg
     2401 tcacaattcg ctgagctatg gtatagtaac taacgttgac atcaatccct gttttaacga
     2461 ggaagaggcg atctaaaaaa gtgaagagca tattggcatt ggcaaagact aacatggctg
     2521 tcagagggag aaagagtggt ttaaaatcac ttaggtgaat cttaacaagt ttgatgtctc
     2581 ttttaatcca aaaataacta atcaggtagt taatcagcgt cgataaactc atcacaagtg
     2641 tatagacaac aatatcgtgt tcatttttaa caaataagaa aatagagacc agcatcagga
     2701 tacggatgaa ggcagttttg taaaagagaa aactgtaatt ttccagagct tcattgaccc
     2761 attcgattga aaaaatctgg gcaatgagtt gaatccccat aacaaggtag acctttttga
     2821 tgattggatt atcagtaaag aagagaggat aggctaggat atagacagca gtggtcaaaa
     2881 tcgtacaagc gatgcacaaa taaaaaagac tagaaaaggt tctgttaaga tcttttttgt
     2941 tatccttgac attactgata gcccttaaac cgtagttata gacaccataa gttgcaaagg
     3001 gcaagaaaaa tgacaaaata gtgtcgactg agttgaagta accatagtca gttcggtcca
     3061 agacacgcgc gacataggtt ccagttagga tgggaaaaat aatattcaag acacgaattc
     3121 ccatgtaaga tagagcattt aattttatac ttttcattca atttacctcg tttttcatta
     3181 tatcataaag ttagctaata agaaatgaag ggcagtaagc caagcaatca ctttgaagtt
     3241 tcaaatctta aattttaagt tttctttaag gaaagtatat tattctgaag gactccaaaa
     3301 tttcgcagtc atttattagt aattgctaca gaattcctag tcattactag aaatggacta
     3361 gtttctttga ataatagaac tgcataattc tcctattcta gaaggggagg accagtattt
     3421 cttttatgat aggactagat tgtggtataa tagagagaat aagttttttt agtaagacaa
     3481 aggagaaaat agatgattta tgcaggaatt cttgccggtg gaactggcac acgcatgggg
     3541 atcagtaact tgccaaaaca atttttagag ctaggtgatc gacctatttt gattcataca
     3601 attgaaaaat ttgtcttgga gccaagtatt gaaaaaattg tagttggtgt tcatggagac
     3661 tgggtttctc atgcagaaga tcttgtagat aaatatcttc ctctttataa ggaacgtatc
     3721 atcattacaa agggtggtgc tgaccgcaat acaagtatta agaaaatcat tgaagccatt
     3781 gatgcttatc gtccgcttac tccagaggat atcgttgtta cccacgattc tgttcgtcca
     3841 tttattacac ttcgcatgat tcaggacaat atccaacttg cccaaaatca tgacgcagtg
     3901 gacacagtgg tagaagcagt tgatactatc gttgaaagta ccaatggtca atttattaca
     3961 gatattccaa atcgtgctca cctttatcaa ggacaaacac ctcaaacatt ccgttgcaag
     4021 gacttcatgg acctttatgg atctctttct gatgaagaga aggaaatctt gacagatgca
     4081 tgtaaaatct ttgtgatcaa aggaaaagat gtggccttgg ccaaaggtga atactcaaat
     4141 ctgaagatta caaccgtaac agatttgaag attgcaaaaa gtatgattga gaaagactag
     4201 taaaatgatt aatcaaattt atcaactaac taagcctaag tttatcaatg tcaaatatca
     4261 ggaagaggct attgaccaag agaatcatat ccttatccgt cccaactaca tggctgtctg
     4321 tcatgcggat cagcgttact atcagggaaa acgtgatccc aagattttga ataaaaagct
     4381 tccaatggca atgattcacg agtcatgtgg aaccgtcatt tctgacccga ccggaaccta
     4441 cgaggttggt caaaaagttg tcatgattcc caatcagtct cctatgcaga gtgatgaaga
     4501 attctatgaa aactacatga cagggaccca tttcttgtct agtggatttg atggctttat
     4561 gagagagttt gtttctctcc ctaaagatcg tgtggtggct tatgatgcta ttgaagatac
     4621 ggttgcagcc attacagagt ttgtcagtgt gggcatgcac gctatgaatc gtctattgac
     4681 tcttgctcat agcaagcggg agcggatcgc cgttattgga gatggaagtt tagcttttgt
     4741 ggttgccaat attatcaact atactttgcc agaatcagag attgtggtta ttggtcgtca
     4801 ttgggaaaag ttggaactct tctcatttgc caaagaatgc tatattacgg ataatattcc
     4861 tgaagagttg gcctttgacc atgcttttga atgttgtggt ggtgatggta ctggaccagc
     4921 tattaatgac ttgattcgct acattcgtcc tcagggaacg attctcatga tgggagttag
     4981 cgaatataaa gtcaatctca atactcgcga tgccttagaa aagggcttga ttttggttgg
     5041 gtcatctcgt tctggtcgca ttgattttaa aaatgctatc caaatgatgg aagtcaagaa
     5101 atttgccaat cgtcttaaaa atatccttta tctagaagaa cctgtaagag aaattaaaga
     5161 tattcatcgt gtctttgcaa ccgatttaaa cacagccttt aaaacagtgt ttaagtggga
     5221 agtataagta ctggaggtta attgtggaga aaatcattaa agaaaaaatt tcttccttac
     5281 ttagtcaaga agaggaagtc ctcagtgttg aacaactggg tggaatgacc aatcaaaact
     5341 atttggccaa aacaacaaat aagcaataca ttgttaaatt ctttggtaaa gggacagaaa
     5401 agcttatcaa tcgacaagat gaaaagtaca atcttgaact actaaaggat ttaggcttag
     5461 atgtaaaaaa ttatcttttt gatattgaag ctggtatcaa agtaaatgag tatatcgaat
     5521 ctgcgattac gcttgattca acgtcaatca agaccaagtt cgacaaaatt gctccaatat
     5581 tacaaactat tcatacgtct gctaaggaat taagaggaga atttgctcct tttgaagaaa
     5641 tcaaaaaata cgaatccttg attgaagaac aaattcctta tgccaactat gaatctgtta
     5701 gaaatgcagt cttctcctta gagaaaagac tggctgactt aggtgttgac agaaaatctt
     5761 gtcatatcga tttggtgcct gaaaacttta tcgaatcacc tcaaggacga ctttatttga
     5821 ttgactggga atattcatca atgaatgatc caatgtggga tttggctgcc ctctttttag
     5881 agtctgaatt cacttcccaa gaggaagaaa ctttcttatc tcactatgag agtgaccaaa
     5941 caccggtttc tcatgaaaag attgctattt ataaaatttt acaagatact atttggagtc
     6001 tatggactgt ctataaggaa gagcaaggtg aagattttgg tgactatggt gtgaatcgtt
     6061 accaaagagc tgttaaaggt ttggcttctt atggaggttc agatgaaaag taaaaacgga
     6121 gttccttttg gccttctctc aggtattttc tggggcttgg gtctaacggt tagtgcttat
     6181 atcttttcga tttttacaga tttgtcaccc tttgtggtgg ctgcagctca tgattttttg
     6241 agcatcttta tcttactagc ttttctcttg gtaaaagaag ggaaagttcg cctctcaatt
     6301 ttcttaaata ttcgcaatgt cagtgttatc atcggagcct tgctagcagg ccctatcggt
     6361 atgcaggcca atctttatgc agttaagtat atcggaagtt ctttagcttc atctgtatcg
     6421 gctatttacc ctgcgatttc agttctattg gctttcttct ttttgaagca caagatttcg
     6481 aaaaatactg tatttgggat tgtcttgatt attggaggga ttattgctca gacctataag
     6541 gttgaacagg ttaattcttt ctacattggg attctttgtg ctttggtttg tgctattgca
     6601 tggggaagtg agagtgttct tagctctttt gccatggaaa gtgaattgag tgaaatcgaa
     6661 gccctcttaa tccgtcaagt aacttcgttc ttgtcctatc ttgtgattgt gctcttctct
     6721 catcagtcat ttactgcagt agccaatgga caattgctag gtctcatgat tgtttttgca
     6781 gcctttgata tgatttccta cttggcttat tatatcgcta tcaatcgctt gcaaccagcc
     6841 aaggctacag gcttgaacgt gagctatgta gtatggacgg tcttgtttgc agttgttttc
     6901 ttgggtgcac cgctagatat gctgaccatt atgacgtcac ttgtcgtcat tgctggagtt
     6961 tatattatta ttaaagaata aaggagattc gtgtgaaagc cattatctta gcagcgggat
     7021 tgggaactcg cttgcgtcct atgactgaaa atacccctaa agccttggtt caggttaatc
     7081 aaaaaccttt gattgagtac caaattgagt ttctcaaaga aaaaggaatc aatgacatca
     7141 tcatcattgt tggttatctt aaagaacaat tcgattactt gaaagagaaa tacggtgttc
     7201 gtctcgtttt caatgataaa tacgctgact acaataactt ttactctctc tatcttgtaa
     7261 aagaagaatt ggccaacagc tatgttattg atgctgacaa ttatctcttt aaaaatatgt
     7321 tccgcaatga tttgacacgt tcgacttatt ttagtgttta tcgtgaagat tgtaccaacg
     7381 aatggttctt ggtttatgga gatgactaca aggttcaaga cattattgtt gatagcaagg
     7441 caggtcgcat ccttagtggt gtatccttct gggatgctcc aactgcagaa aagattgtca
     7501 gctttatcga caaggcttat gtaagtggtg aatttgttga tctctattgg gacaatatgg
     7561 ttaaggataa tatcaaagag ctagatgtct atgttgaaga attagaaggc aatagcattt
     7621 atgagatcga tagtgtccaa gactatcgta aattagaaga aattcttaaa aacgaaaatt
     7681 aaagattcca acatctgaca aaatagtcgg atgttttttg attttttacg aacttttacg
     7741 aatagataga tgagtagaaa aagaaatgga gttatttatg aaaatcacaa actatgaaat
     7801 ctataagtta aaaaaatcag gtttgaccaa tcaacagatt ttgaaagtgc tagaatacgg
     7861 tgaaaatgtt gatcaggagc ttttgttggg tgatattgca gatatctcag gttgccgtaa
     7921 tccagccgtt tttatggaac gttattttca gatagacgat gcgcatttgt cgaaagagtt
     7981 tcaaaaattt ccatctttct ctattttaga tgactgttat ccttgggatt tgagtgaaat
     8041 atatgatgcg cctgtacttt tattttacaa gggaaatctt gacctcctga aattcccgaa
     8101 ggtagcggtc gtgggcagtc gtgcttgtag caaacaggga gctaagtcag ttgaaaaagt
     8161 cattcaaggc ttggaaaatg aactggttat tgtcagtggt ctggccaagg gcattgacac
     8221 agcagctcat atggcagctc ttcagaatgg cggaaaaacc attgcagtga ttggaacagg
     8281 actggatgtg ttttatccta aagccaataa acgcttgcaa gactacatcg gcaatgacca
     8341 tctggttcta agtgaatatg gacctggtga acaacctctg aaatttcatt ttcctgcccg
     8401 taatcgcatc attgctggac tttgtcgtgg tgtgattgta gcagaggcta agatgcgttc
     8461 aggtagtctc attacgtgtg agcgagcaat ggaagaagga cgcgatgtct ttgctattcc
     8521 tggtagcatt ttagatggac tatcagacgg ttgccatcat ttgattcaag aaggagcaaa
     8581 attagtcacc agtgggcaag atgttcttgc ggaatttgaa ttttaaaaat gacctaagct
     8641 agaattctaa gaaaaaatca attttaagag aaaatgaacc caacatttcc ataataaaac
     8701 gcatattagc aagtttttaa cacttgataa tatgcgtttt ttctaagtgg attagtagag
     8761 tagaggattt ttctcatata atactcttcg aaaatctctt caaactacgt cagcttccat
     8821 ctgcaacctc aaaacagtat tttgagctga cttcgtcagt cttatctaca acctcaaagc
     8881 agtgctttga gcaacctgtg gctagcttcc tagtttgcgc tttgattttc attgagtata
     8941 agggaaagta tagtgaattg aaataagatg tgaacaactc tatcaggaaa gtcaaattga
     9001 tttatagaaa tattttagca gccaaggtgc actgttatag attcaataca ctataattta
     9061 gtgtaattga gaaaggagaa atgattgtga ttgatgttgg ctaggttatg ttcaatgatt
     9121 cctaccgtct caaatcttgt cagtaaggaa aaataaattc ttcaaaagta gagattacaa
     9181 ggcttgttta agaaagaatt caaagacctt gacaaataaa aataaaatgg ttattataaa
     9241 aaatggtctg aaatagatga tgatactttt cgaaaatctc ttcaaactac gtcagctcag
     9301 ctttgccttg ctgtgttttg agcaagctac ggttagcttc cgagtttgat tttcatttac
     9361 tagaaatgaa actgatgaga gatatcagta gacatttgag tcaggatatt atggaaaatg
     9421 ataaaaagag ctcgtgagat tggcatatca gactactaaa gtattgagtt tgttaggatt
     9481 ttagcgacta gttagctggg aaaggaagat atttgtgaca aataataaac tgtattcgtt
     9541 gatagaattt agaaataaaa tatatgaaga attagaactt tccagaagtg atttagcgat
     9601 tttactatgt gccatgctta tcgcctctat cggattaaat atggattcga ctcccgtgat
     9661 tattggagcc atgttaatct ctcctttgat gacacctatt ctgggagtgg ggctctctct
     9721 agctatattt gattttaaat tgttaagaaa atcttttaaa atattagcta ttcaaattct
     9781 tgccagtcta atagcttcaa cactttattt ttatctttct cccatttcgt atgctagttc
     9841 ggagattgtt gctagaacct ctccgactat ttgggatgtt ctcattgctt ttgtaggagg
     9901 gatagcaggt attattggtg ctaggaaaaa agagaccaat aatattgttc ctggtgttgc
     9961 tattgcaacc gccttgatgc ctcctctttg tacagtaggt tatgctattg cttctgctaa
    10021 tctaaaattt atcataggct cctcttacct attcctcatc aattgtagct ttattgtcat
    10081 tgcgacttat ataggtgtta ggttgatgat ggttaagaaa cattatttta aagataatga
    10141 agaagactct aaaatgcgta ggattttgct tctagttgct gttttgctga tgattccgag
    10201 tttcatctct gcaacgactt tagtgagaga aacgttgaaa aaagagtccc ttaagaaatt
    10261 tatatcagag cagtttcagg ggtataatat tttgaaaaaa acctattcta aaaagactca
    10321 taccctaaag ctaaccattt caggaaatta tttgacagaa gaagaactcg atatgatttc
    10381 aagtaagaga ggtgactatg gtttaagtga tgtttctgtt caagtttcac aattgtctga
    10441 ttcagaacaa cttagtaagg aagagctggt ggagtatttc ttccagtata tcaaggataa
    10501 ggaagcaaaa gaaaaggaga aagccaataa atttgataca gagtctgagg agcaataatt
    10561 tcttgagaat agctggtttc tctcgtgagt cttctatgta tatcaaagga agactgaggt
    10621 tttaagtata aaacttttct tctattatag tagctaagtc ttgacagggt tcaaaaaatg
    10681 gtttacactt tataaagttt attactttga aaaggtgtga tactgtggct acggcaacaa
    10741 aaaagaaaaa atcaacagtt aaaaaaaatc tagtcatcgt ggagtcgcct gctaaggcca
    10801 agacgattga aaaatatcta ggcagaaact acaaggtttt agccagtgtc gggcatatcc
    10861 gtgatttgaa gaaatccagt atgtccgtcg atattgaaaa taattatgaa ccgcaatata
    10921 ttaatatccg aggaaaaggc cctcttatca atgacttgaa aaaagaagct aaaaaagcta
    10981 ataaagtttt tctcgcgagt gacccggacc gtgaaggaga agcgatttct tggcatttgg
    11041 cccatattct caacttggat gaaaatgatg ccaaccgtgt ggtcttcaat gaaatcacca
    11101 aggatgcagt caaaaatgct tttaaagaac ctcgtaagat cgatatggac ttggtcgatg
    11161 cccaacaagc tcgtcggatc ttggatcgct tggtagggta ttcgatttcg cctattttgt
    11221 ggaagaaggt caagaagggc ttgtcagcag gtcgcgttca gtccattgcc cttaaactca
    11281 tcattgaccg tgaaaatgaa atcaatgcct tccagccaga agaatactgg acagttgatg
    11341 ctgtctttaa aaagggaacc aaacaatttc atgcttcctt ctatggagta gatggtaaaa
    11401 agatgaaact gaccagcaat aacgaagtca aggaagtctt gtctcgtctg acgagtaaag
    11461 acttttcagt agatcaggtg gataagaaag agcgcaagcg caatgctcct ttaccctata
    11521 ccacttcatc tatgcagatg gatgctgcca ataaaatcaa tttccgtact cgaaaaacca
    11581 tgatggttgc ccaacagctc tatgaaggaa ttaatatcgg ttctggtgtt caaggtttga
    11641 ttacctatat gcgtaccgat tcgactcgta tcagtcctgt agcgcaaaat gaggcggcaa
    11701 gcttcattac ggatcgtttt ggtagcaagt attctaagca cggtagcaag gtcaaaaacg
    11761 catcaggtgc tcaggatgcc catgaggcta ttcgtccgtc aagtgtcttt aatacaccag
    11821 aaagcatcgc taagtatctg gacaaggatc agcttaagct atataccctt atctggaatc
    11881 gttttgtggc tagccagatg acagcggccg tttttgatac catggctgtt aaattgtctc
    11941 aaaaaggggt tcaatttgct gccaatggta gtcaggttaa gtttgatggt tatcttgcca
    12001 tttataatga ttctgacaag aataagatgt taccggacat ggttgttgga gatgtggtca
    12061 aacaggtcaa tagcaaacca gagcaacatt tcacccaacc gcctgcccgt tattctgaag
    12121 caacactgat taaaacctta gaggaaaatg gggttggacg tccatcaacc tatgcgccaa
    12181 ccattgaaac cattcagaaa cgttattatg ttcgcctggc agccaaacgt tttgaaccga
    12241 cagagttggg agaaattgtc aataagctca tcgttgaata tttcccagat atcgtaaacg
    12301 tgaccttcac agctgaaatg gaaggtaaac tggatgatgt cgaagttgga aaagagcagt
    12361 ggcgacgggt cattgatgcc ttttacaaac cattctctaa agaagttgcc aaggctgaag
    12421 aagaaatgga aaaaatccag attaaggatg aaccagctgg atttgactgt gaagtgtgtg
    12481 gcagtccaat ggtcattaaa cttggtcgtt ttggtaaatt ctacgcttgt agcaatttcc
    12541 cagattgccg tcatacccaa gcaatcgtga aagagattgg tgttgagtgt ccaagctgtc
    12601 atcagggaca aattattgag cgaaaaacca agcgtaatcg cctcttctat ggttgcaatc
    12661 gctatccaga atgtgaattt acctcttggg acaagcctgt tggtcgtgac tgtccaaaat
    12721 gtggcaactt cctcatggag aaaaaagtcc gtggtggtgg caagcaggtt gtttgtagca
    12781 aaggcgacta cgaggaagaa aagattaaat aagaggaggg gcccgaaagt aaatttcagg
    12841 ctccttttgg ttagaacttg acaaaataca tcattttatg cgaaactaga agaagattat
    12901 tttaactagg agaagttatg cgtcttatct atctgataat tggtttttta tcactggcct
    12961 tggctattgt tggggttgtt ttacccttgt tgcctacaac acctttcctt ttgttgtcta
    13021 ttgcttgttt ctccagaagt tccaagcgat tcgaagactg gctttatcat accaagctct
    13081 atcaaacata tgtagctgat tttcgcgaga ctaagtctat tacgcgtgaa cgaaagaaaa
    13141 aaatcatcgt atccatttac attttgatgg ggatttctat ttattttgcc cctctcttac
    13201 cagtcaaaat cggtctgggt gctttgacca tctttatcac ttattatctc ttcaaggtca
    13261 ttccagacaa agaatagtta aaacagtagt tatttacctt gataaaattg aaagcatatt
    13321 cataacaata tgatataata aatttgaagt aatattcaag gagaatcaaa tgatttacga
    13381 attttgtgct gaaaatgtga ctttacttga aaaagcgatg caggctggag ctcgtcggat
    13441 tgaactctgt gataatctag cagttggtgg gacaacacct agctatggag tgactaaggc
    13501 agcggttgaa ctggcagcta actacgatac aaccatcatg accatgattc ggccacgtgg
    13561 tggtgacttt gtctataatg acctagaaat tgctatcatg ctagaagaca tttgtttgac
    13621 tgctcaggct ggaagtcaag gggttgtatt tggagcttta actgctgata aaaagttgga
    13681 taagcctaat ctggaaaagt taattgctgc atcaaaagga atggaaattg tctttcacat
    13741 ggcctttgat gaactaagtg atgaagatca accggaagct attgactggc tcagtcaagc
    13801 cggtgtcact cgtatcctaa ctcgtgctgg tgtgtctggc gactccttag aaaaacgttt
    13861 tgttcactat cacagaattt tggagtacgc taaaggtaaa attgaaattc taccaggtgg
    13921 ggggattgac cttgacaacc gtcaaacctt tattgaccag gtgggggtaa cacaattgca
    13981 tggtactaag gttgtttttt aaaaaataga aaggaactgc tagccttggg tagcagtttt
    14041 cacttatgtt tgaaattttt aaatcctatc aatttaatca agaaaaggct catgattatg
    14101 gttttataga aaatagcgaa gtctggacat atagttgcca gattttgcaa ggtgactttg
    14161 tcatgactgt gtccatcact gctgataatg tgaactttca agtctttgac caagagactg
    14221 gtgacctcta tcctcacgtt catatggaaa gcatgagggg aagttttgtc ggaaatgtcc
    14281 gtgaggcttg tctggagatt ctttaccaga ttcggaaggc ttgttttgat gtgcaagatt
    14341 ttatctgtca tcagactaag cgtatcatga ctcaagttca ggaaaagtat ggaaaccagt
    14401 tggagtatct gtgggaaaaa tcgcctgata cagctgtatt gcgccatgaa ggcaatcaaa
    14461 agtggtatgc cgtcttgatg aaaatctctt ggaataagct ggaaaagggc agagaaggac
    14521 aagtggaagc agtcaacctc aagcatgacc aagtagctaa tttgctttca caaaagggga
    14581 tttatccagc cttccatatg agcaagcgct actggattag tgtgtccctt gatgatactt
    14641 tatcagatga agaagtactg gaattgatag aaaaaagttg gaacttaacc tctaaaaaat
    14701 gaaatatttt aataattttc atgaactttc aattagctaa atattcttta ctgaagaaat
    14761 ttttagaaaa tataggattt accacactag aggaatatgg tgccatcttc aaatacctga
    14821 ttgagaatgt caagacggat cgtcagatca tctgttcgcc tcactgtcat gatgacctcg
    14881 gaatggcagt ggcaaatagc cttgctgctg tcaagaatgg tgcaggacgt gttgaaggga
    14941 ctatcaatgg tattagggag cgagctgaaa atgctgcttt ggaagaaatt gcagtggctc
    15001 tcaatattcg ccaagattac taccaagtag aaaccagtat tgtcctaaat gagaccatca
    15061 atacgtcaga aatggtttct cgcttctctg gtattccagt tcctaaaaac aaagccgtcg
    15121 ttggtggcaa taccttctcc cacgaatctg gtattcacca agatggagtc cttaaaaatc
    15181 ctctcactta tgagatcatc acacctgaat tggttggtgt taagattccg cttggaaaat
    15241 tatctggtcg ccatgctttt gttgagaaac tgagagaatt ggccctagat tttacagaag
    15301 aggatatcaa accactcttt gctaagttca aggcactggc cgataagaag taagaaatca
    15361 cagatgcaga tcctcgagct ttggtagctg gaaccatggt tgaaaatcca gaaggcttcc
    15421 actttgatga tttacaactt caaactcatg cagataatga cattgaagcg ctcgttagcc
    15481 tagccaatat ggatggtgag aaagtcgaat ttaatgcgac agggcaaggt tccgttgaag
    15541 caatctttaa cgctatcgat aagttcttta accaatctgt tcgtttggtg tcctacacta
    15601 tcaatgcggt aacagatgga atcgatgccc aggatcgggt tttggtcact gttgaaaaca
    15661 gagatacaga aaccatcttt aatgcagcag ggcttgattt tgatgtgttg aaggcttctg
    15721 ctattgccta tataaacgct aatacctttg ttcaaaaaga gaatgcaggt gagatgggac
    15781 gcagtgtttc ttaccacgat atgcctagtg tgtaaaggag aaggctatgg caaagaaaat
    15841 agtagctcta gtaggagacg gaattggccc agaaatcatg taggctggtt tagaagttct
    15901 ggaggctcta gctgaaaaaa caggttttga ctatgagatt gacagacgac cgttcggagg
    15961 tgcagatatt gatgcagcag gacctccctt acctgatgaa acccttaagg caagtaggga
    16021 agcagatgct atcctactag cagctatcgg tagtcctcag tatgatggag cagcggttcg
    16081 ccctgaacaa ggcctgatgg ctctccgtaa ggaactcaat ctttacgcta atattcgtcc
    16141 tgtaaaaatc tttgacagtc tcaagtattt gtcaccactc aaaccggaac gaatttctgg
    16201 tgtagacttt gtcgtggtgc gtgaattgac aggcgagatt tactttggag atcatattct
    16261 tgaagagcgc aaagcgcgtg atatcaacga ctatagctat gaggaagtgg agcggattat
    16321 tcgcaaagcc tttgaaattg caagaaatcg cagaaaaatc gttactagta tcgataagca
    16381 aaatgttcta gcgacctcaa aactctggcg gaaagtagct gaggaagtcg cacaggattt
    16441 cccagatgta accttggaac accagctggt agactcagct gctatgctta tgattaccaa
    16501 tcctgctaag tttgatgtta ttgtaacgga gaatcttttt ggagatattt tatctgatga
    16561 atcaagcgtc ttatctggta cacttgaggt tatgccatca gccagtcatt ctgaaaatgg
    16621 accaagtctc tatgaaccta ttcacggttc agcacctgat attgcaggtc aaggaattgc
    16681 caatcctact tccatgattt tatcagttgc catgatgttg agagatagtt tcggacgtta
    16741 tgaggatgca gagcgtatca aacatgctgt tgagacaagt ctggcggcag gaattttaac
    16801 gagagatata ggaggtcagg cttcaacaaa ggaaatgacg gaagctatta ttgcaaggtt
    16861 atgaagttag acgaaaaaat tactctagtc cttttgattt ggaatgtcat cattttcttg
    16921 atttatggca ttgacaaatt caaggcaaga agaagaactt ggcgcatcca agagaaaatc
    16981 ttactcattt tagcccttac ttgtggtggt tttggggcct ggttagctgg aatcactttt
    17041 caccacaaga ctcgaaaatg gtactttaaa acagtttggt ttcttgggat ggtgaccaca
    17101 ctagtagcct tatattttat ttggaggtaa tggatggcag ggtcttcgag ggaacacgct
    17161 gcttgggttc tagcggacta tggttttaag gtcgtgattg caggatcttt cggtgacatt
    17221 cattacaata atgaactcaa taatggcctg ttgccaatcg ttcagcctag agaggttaga
    17281 gagaaactag cccagctaaa accaaccgac caggtaactg tggacttgga acaacaaaaa
    17341 atcatctcac cagttgaaga attcaccttc gagatagata gcgagtggaa acataaactc
    17401 ctaaatagtt tggatgatat cggtattacc ttgcagtatg aagagttgat tgctgcttat
    17461 gaaaaacaac gaccagccta ctggcaggat tagaaaaaat agaaaaggaa atatagtaaa
    17521 ctgaaataag atgtaaacaa atgaattaga gcttaacatc catttccagc aattttttag
    17581 aaactacagt ggactattct ggattcaaca cattataaaa ttatgacaaa acacattcac
    17641 aagaaggcta cgacatttta aaaggtgagg gcggatgtat cgtttgccct actaaagttg
    17701 gttacattat catgaccagc aacaaggcag gacttgagcg taagttcgca gccaaagaac
    17761 gtaagcgtaa caaaccaggt gttgttctct gcggtagcat ggatgaactt cgcgctttag
    17821 cgcaactcaa cccagaaatt gaagcattct actaaaaaca ttggatgaag atattcttct
    17881 tggttgtatc cttccttgga aaccagaagc ctttgaaaaa ctcaaagcat acgggaatgg
    17941 ccgtgaagaa cttatgactg atgtacgtgg tactagctgt tttgttatca agtttggaaa
    18001 agcaggtgaa caattggctg ccaagctttg ggaagaaggt aaaatggtct acgcctcata
    18061 tgcttcaatg acaaaacgat tgaaactcgc tatgagagca aggtgtaatg gtgtctatgg
    18121 tcgataagga cggcaaactc atcccagaac aaggaggagc acgttcaact tcaccagcac
    18181 cagttgtgat ccgtaaaggg cttgacattg ataaaatcat gatgcacctg tcagatactt
    18241 ttaactcatg ggactaccgt caggttgagt attattaaga taaaaaaaga agtctagtgt
    18301 tatgagatat taaagctcct aacactggac tttttgttta gaatttcttt tctttttcta
    18361 taggatatgg ta
//
LOCUS       DAVLVF010000030        17319 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVLVF010000030 DAVLVF010000000
VERSION     DAVLVF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.030"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            215..898
                     /gene="rpiA"
                     /locus_tag="VYN66_001056"
     CDS             215..898
                     /gene="rpiA"
                     /locus_tag="VYN66_001056"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV1495094.1"
                     /translation="MENLKKMAGIKAAEFVKDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQIIAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRRFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            912..2123
                     /locus_tag="VYN66_001057"
     CDS             912..2123
                     /locus_tag="VYN66_001057"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV1495095.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            2125..2658
                     /locus_tag="VYN66_001058"
                     /pseudo
     CDS             2125..2658
                     /locus_tag="VYN66_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285096.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            2682..3491
                     /locus_tag="VYN66_001059"
     CDS             2682..3491
                     /locus_tag="VYN66_001059"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1495096.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            3967..4362
                     /locus_tag="VYN66_001060"
     CDS             3967..4362
                     /locus_tag="VYN66_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEV1495097.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            4372..4947
                     /locus_tag="VYN66_001061"
     CDS             4372..4947
                     /locus_tag="VYN66_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1495098.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            4972..5484
                     /locus_tag="VYN66_001062"
     CDS             4972..5484
                     /locus_tag="VYN66_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV1495099.1"
                     /translation="MSAQITINHKKARYVRIGLEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNFPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            6142..6852
                     /gene="deoD"
                     /locus_tag="VYN66_001063"
     CDS             6142..6852
                     /gene="deoD"
                     /locus_tag="VYN66_001063"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1495100.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(6875..7399)
                     /locus_tag="VYN66_001064"
     CDS             complement(6875..7399)
                     /locus_tag="VYN66_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV1495101.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(7452..7688)
                     /locus_tag="VYN66_001065"
     CDS             complement(7452..7688)
                     /locus_tag="VYN66_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV1495102.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSTKLAK"
     gene            complement(7756..8676)
                     /locus_tag="VYN66_001066"
     CDS             complement(7756..8676)
                     /locus_tag="VYN66_001066"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV1495103.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            8867..9270
                     /locus_tag="VYN66_001067"
                     /pseudo
     CDS             8867..9270
                     /locus_tag="VYN66_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9486..10067
                     /locus_tag="VYN66_001068"
     CDS             9486..10067
                     /locus_tag="VYN66_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1495104.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            10064..11341
                     /locus_tag="VYN66_001069"
     CDS             10064..11341
                     /locus_tag="VYN66_001069"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV1495105.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDVLDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            11359..12021
                     /gene="deoC"
                     /locus_tag="VYN66_001070"
     CDS             11359..12021
                     /gene="deoC"
                     /locus_tag="VYN66_001070"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV1495106.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSTVKAFETKEAIQNGADEIDMVINVGALKSGDL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVLACQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGPDMGVKAAGGARSYADALTFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            12008..12397
                     /locus_tag="VYN66_001071"
     CDS             12008..12397
                     /locus_tag="VYN66_001071"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1495107.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            12488..13540
                     /locus_tag="VYN66_001072"
     CDS             12488..13540
                     /locus_tag="VYN66_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV1495108.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQDWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            13684..15219
                     /locus_tag="VYN66_001073"
     CDS             13684..15219
                     /locus_tag="VYN66_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495109.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGAINADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQGLGVLMAGGNLGKEK
                     SDV"
     gene            15212..16270
                     /locus_tag="VYN66_001074"
     CDS             15212..16270
                     /locus_tag="VYN66_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1495110.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            16273..17229
                     /locus_tag="VYN66_001075"
     CDS             16273..17229
                     /locus_tag="VYN66_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1495111.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         5065 a         3084 c         3971 g         5199 t
ORIGIN      
        1 aaactaaatt tgttcgtttt tttactcttg taaatctatt tttgttagag ttgatttggt
       61 ttacatccgt acttaaagtg atttgtcaga gttctgcttt tattaaaaaa atttaatttc
      121 aaggataaat aagcagtatt ctaaaggtac ttttagatga aataaaagcc tttacatggt
      181 ataatagagg tagctcttta atggaggtgt ttgagtggaa aatctgaaga aaatggcagg
      241 tatcaaggct gctgagtttg tcaaggatgg aatggtcgtt ggacttggaa caggctcgac
      301 tgcctattat tttgtcgaag aaatcggtcg tcgtatcaag gaagaaggat tgcagattat
      361 agctgtaacg acttctagtg tgaccactaa acaggcagaa ggccttaata tcccactcaa
      421 atctattgac caagtagact ttgtcgatgt gacagttgat ggcgcagatg aagtggatag
      481 tcagtttaat ggaatcaaag gtggtggtgg tgctcttctg atggagaagg ttgttgcgac
      541 accatcaaaa gaatacattt gggtggtgga tgaaagcaag ctggtcgaaa aactaggtgc
      601 ttttaaattg ccagtagaag tggttcagta tggtgcagaa caggtctttc gtcgttttga
      661 acgagctggc tacaaaccaa gtttccgtga aaaagacggc caacgttttg tgacggatat
      721 gcagaatttt atcattgacc tcgccttgga tgtcattgaa aatccaattg cttttggaca
      781 agaattggac catgtcgttg gtgttgtgga gcatggttta ttcaaccaaa tggtggataa
      841 ggtcatcgtt gctggacggg atggagttca gatttcaact tcaaaaaaag gaaaatagaa
      901 gggggcataa gatgtctaaa tttaatcgta ttcatttggt ggtactggat tctgtaggaa
      961 tcggtgcagc accagatgct aataactttg tcaatgcagg ggttccagat ggagcttctg
     1021 acacactggg acacatttca aaaacagttg gtttgaatgt cccaaacatg gctaaaatag
     1081 gtcttggaaa tattcctcgt gaaactcctc ttaagactgt agcagctgaa agcaatccaa
     1141 ctggatatgc aacaaaatta gaggaagtat ctcttggtaa ggatactatg actggacact
     1201 gggaaatcat gggactcaac attactgagc ctttcgatac tttctggaac ggattcccag
     1261 aagaaatcct gacaaaaatc gaagaattct caggacgcaa ggttattcgt gaagccaaca
     1321 aaccttattc aggaacggct gttatcgatg attttggacc acgtcagatg gaaactggag
     1381 agttgattat ctatacttca gctgaccctg ttttgcagat tgctgcccac gaagacatta
     1441 ttcctttgga tgaattgtac cgtatctgtg aatacgctcg ttcgattacc cttgagcgtc
     1501 ctgcccttct tggtcgcatc attgctcgcc cttatgtagg tgaaccaggt aacttcactc
     1561 gtacggcaaa ccgtcgtgac ttggctgtat ctccattttc cccaactgtt ttggataaat
     1621 tgaatgaggc tggtatcgat acttatgctg tgggtaaaat caacgatatc tttaacggtg
     1681 ctggtatcaa ccatgacatg ggtcacaaca agtcaaatag tcatggaatt gatacactat
     1741 tgaagactat gggacttgct gagtttgaaa aaggattctc attcacaaac ctagttgact
     1801 ttgatgccct ttacggccat cgtcgtaatg ctcacggtta ccgtgattgc ttgcatgagt
     1861 ttgatgaacg cttacctgaa attatcgcag ctatgagaga gaatgacctt ctcttgatta
     1921 ctgcggacca tggaaatgac ccaacgtatg caggaacgga tcacactcgg gaatatattc
     1981 cattgttggc ctatagccct gcctttaaag gaaatggtct cattccagta ggacattttg
     2041 cagatatttc agcgactgtt gccgataact ttggtgtgga aactgctatg attggggaaa
     2101 gtttcttaga taaattggta taagatgacg cgctatgctt tgctggtgag aggtatcaat
     2161 gttggtggta agaataaggt cgtcatggcg gagcttcgtc aagaattgac aaacttggga
     2221 ctggaaaagg ttgagagcta catcaacagt ggcaatattt tctttacttc gatagattcc
     2281 aaagcccaat tggttgaaaa gctagagact ttctttgcag tccattatcc atttattcag
     2341 agcttttctt tactgagtct agaggacttt gaggcggaac ttgaaaatct accagcttgg
     2401 tggagcagag atttggcacg aaaagatttt ctcttttaca ctgagggttt ggatgtggac
     2461 caagtcatcg cgacagttga aagtttagag ctgaaagatg aagtgcttta ttttggaaaa
     2521 cttgggattt tctaggggaa attttctgaa gaatcctatt ctaagactgc ctatcataag
     2581 tacttgctga ggtgcctttc taccgccaca ttactattcg taatgctaaa acctttgaca
     2641 aaattggtca aatgctaaaa aaataataaa ggagacacac aatgacattt ttaaacaaaa
     2701 tccatgaaac tgctactttc ctgaaagaaa agggaattgc agcccctgag ttcggtctaa
     2761 tccttgggtc aggacttgga gaattggcag aagaaatcga aaatccagtt gtagtagact
     2821 atgctgagat tccaaactgg ggccgttcaa cagtagtcgg tcatgctggt aaattggtat
     2881 atggtgaact ggcaggtcgc aaggtcttgg ctcttcaagg gcgtttccat ttctatgaag
     2941 ggaatcctct ggaagtggtg actttcccag ttcgtgtgat gaaagttctt ggatgtgaag
     3001 gtgttattgt aaccaatgca gctggcggta tcggatttgg tcctggtacc ttgatggcta
     3061 tctcagacca tatcaacatg acggggcaaa atccattgat gggtgaaaac ttggatgact
     3121 ttggcccacg tttcccagat atgtctaggg cctacacacc agaataccgt gctactgccc
     3181 atgaagtggc taaaaaactt aatatcaagc ttgatgaagg tgtctatatc ggagttactg
     3241 gtccgactta tgaaacacca gcagaaattc gttcctataa gacactggga gcagatgcag
     3301 ttggtatgtc tacggttcct gaagttatcg tggcagccca ctctggcttg aaagttctgg
     3361 gaatttcatg tatcactaac tttgcggctg gtttccaaga agaactcaat cacgaagaag
     3421 ttgtagaagt gactgaacgt gttaaaggtg attttaaagg cttgcttaaa gcgattcttg
     3481 ctgaattgta agaaaaaaga tttaaaaggg ggagtgcctc tgtcttttca ggattgactg
     3541 cctatccgta ttaaagaaga aacagaggaa tactatgagc ttcttcctgc tcttataact
     3601 gaaagaagcg gaagaatagg tgtgtctgat ctgatagcca gcattgtgaa agacaagatt
     3661 ctaggatact agcattagct tcctagccaa gcagactagt atgataagga gagatgagaa
     3721 tgaattgact ttctgaattt ctcagtctta tcatatatag cacaatgaga tttcgcttga
     3781 gtctgcttgt aaataaacga aaagaaagat aagaaataat gaaaattggt caacgaatta
     3841 tgcgctttgg cataaaaaat taagtatcgg agttgtatct gttgtagtcg gctttgattt
     3901 ctagctccag ctggaatttc agccaatgaa gtaaagcaag atgtaacaac tgaagtggta
     3961 ataggtgtgc tagattctaa ggaggaattg aaagagtcag aaaatgatgc tccaaaacta
     4021 gaaactcctc ttagagagga gccaagacta gctcctcaaa cgcttccgga agcaagtgaa
     4081 gttcttgaaa acaaaaggga agagtcaaaa gtagagataa cagagccagc tcaagcggat
     4141 gacatccgca aggttgttgg ggaattagcc aaggatataa gtattactaa gttgtatatg
     4201 acaggtcatt ctcttggagg ctacctagct cagattgcag cggttgaaga ttaccaaaaa
     4261 tatcctgatt tttataacca tgtattgagg aaagtgacaa ctttcagtgc tcctaaagtg
     4321 attacttcca gaactgtttg ggatgctaag aatggtttct gagatgttgg tttggaaagt
     4381 cgtaaattag ctgttagtgg aaaaattaag cattatgtgg ttgataatga caatgttgtg
     4441 actcccttga ttcataataa tcgtgatatt gttacattta caggtaattc acgctttaaa
     4501 caccgttctc gtggctattt tgaaagtcca atgaatgata ttcctaactt taatattggt
     4561 aaacaagcta ccttggataa acatggttat cgtgatccga aattggataa agtgcgattc
     4621 tttaagaaac aggctctacc tcaatcttct agtcaaccaa gcgctgaacc aatggaaaat
     4681 attgccttag gaaaacaggt tactcaaagt tcgacagctt tcggaggaga tgctagaaga
     4741 gctgtggatg gcaaagtcga tggtaactat ggtcacaatt ctgtcactca tacaaacttc
     4801 caatctaagc cttggtggca agtagatttg gctaaagaag aaaccattcg ccaaatcaat
     4861 atttacaacc gaacagacac tgcccaggat agattggcaa actttgatgt cattctttta
     4921 gacagttctg gtaaagaaat tgagtgaaaa cgtataatat ctcctaaaga tgtgtcagca
     4981 caaattacga ttaaccataa aaaagcgcgc tatgttcgga ttgggctaga aggctataat
     5041 gccctcagtc ttgcagaagt tgaagttttc cgctttatag ctacgaatgc tgaaacggcg
     5101 acacaagttt ctaagccagt tcaaccaatc agtcagactc ctgtgaaaga taaaacattg
     5161 acaattcaac acagtggagc ttacattgcc cgctactcca taacttggga agaagttcca
     5221 gtagataaag atggaaacca agttgttcgt agtcattctt gggaaggaaa cggttgcaac
     5281 cagactgcag gttttgtcct caacttccca atcaaagaaa atatgagaaa tctgcgagtt
     5341 aagattgaga aaaagacggg cctactatgg aatagatggc aaacaatcta tgaaaacaga
     5401 ccaattttag ctcaacccca ccgtaaaatt acccattggg gtacgacatt gaattccaag
     5461 gtgagtgacg atgatgtctt gtaatctgat ggtacaatga cagttagttt gtctagttta
     5521 taagaaagta ctatctgagc ttgaatagaa ctcaggtagc tctctgtgaa agaacaaaat
     5581 taatactcaa tgaaaatcaa agagcaaact aggaaactag ccgcaggttg ctcaaagcac
     5641 tgctttgagg ttgtagataa gactgacgaa gtcagtcaca tatataatcc aaggcgacgt
     5701 tgacgtggtt tgaagagatt ttcgaagagt ataaacagaa aggtagagcg cgtgttctaa
     5761 tttgaacacg agtagaaaac ttttctaaaa acaaaaacga aaggatgggt aaactgtatt
     5821 cgctgaactg aatacgggcg actctcctct aaatcaaaat taagaaagaa aggaattgac
     5881 cccaccctaa aagcagtggg aaaaagatag ttggtctagc gagcatcgct cactgcgccc
     5941 aactcctatt ttcccttcgc tttttgacgg gtttggtatc tttctcaata taaaatataa
     6001 aataaagaaa ggtagagcgt gtgttttgat ttgaacacga gcggaaaact cggaaaatag
     6061 ataatctgac tgaaaaatca ggatttctcg tcggcttcct aattttcagt cgttttcttc
     6121 tcgctctttg tatcataaat tatgtctatc catattgctg ctcagcaggg tgaaattgct
     6181 gataaaattc ttcttcctgg ggatcctctt cgtgctaagt ttattgcgga gaatttcctt
     6241 gatgatgctg tttgttttaa cgaagtgcgt aacatgtttg gttacactgg tacttacaag
     6301 ggtcaccgtg tatctgtcat gggaactggg atgggaatgc catctatttc gatttatgcg
     6361 cgtgagttaa tcgtagacta cggtgtgaag aaattgattc gtgtgggaac tgcaggttct
     6421 ttgaatgaag aggttcatgt tcgtgaatta gttttggcgc aggcggctgc aaccaactca
     6481 aacatcgttc gtaatgactg gccacagtac gattttccac aaattgctag ctttgatttg
     6541 cttgataaag cctaccatat cgccaaagaa cttggtatga ctactcacgt tgggaacgtt
     6601 ttgtcatctg atgtctttta ctcaaattac tttgaaaaga atatcgagct tggtaaatgg
     6661 ggagtcaagg ctgtggaaat ggaagcagca gctctttact atcttgctgc ccaataccat
     6721 gttgatgcgc tagctatcat gaccatctct gatagcttgg tcaatccaga cgaagacaca
     6781 actgcagaag aacgtcaaaa taccttcact gatatgatga aggttggttt ggaaaccttg
     6841 attgcagaat aattatagcc aaaaaggaag ccctttatga attgtaggac ttccttttct
     6901 tatccagaaa ttgatctagc tctctctgat ttcgaagaat agtgacttta tgtgaatatt
     6961 cttggcaaag tttttggtaa ttttcttttt gagttttgct acgcccatcc caaagaatcc
     7021 atctgataaa ctcccactca aagcgttcag ggcaatctgc cgccatactt tctctgactt
     7081 ttccacggta tttaagataa cgcttaaagg ctctaaagag acaggtcaat ggcaaaaaat
     7141 tgagaaagat gatttggtca gcttcttgca ttcgttcttg gtagtagcac caagaataat
     7201 taccatcgat gacccaagct ttatgcttgg tgagaaagtt ttttatctcg gttaacatcc
     7261 attcgcagtc actgtcttgc caaccaggtt gaaattggag tgtgtccatg tgcagttttg
     7321 gaatggagta gtagttagat aacttttctg ctagagttga cttaccagaa ccagaatatc
     7381 cgataattgt gattttcatt ttctaccttt tcctatttgg agacaaaaaa acagcctcta
     7441 tggactgttt cttatttagc aagtttagtt gaaagacgag ctttatcgcg gcttgctttg
     7501 tttttgtgaa tcaaaccttt agtttctgct ttatcgatag ctgagctagc agcacggaaa
     7561 agttcttcag atgggtttgc ttcgaaagct tttatagcag tacgcatagc tgatttttga
     7621 gctgagttct tttcgttttg tttaacgttc aattcagcgc gtttgatagc tgatttaatg
     7681 tttgccaatg ttcttacctc catatttact aactatacca ttatatatga aaacttacgt
     7741 tttgacaagg gaaaattact tttttaagta aatttcatcg atttcatggt tcttgctttt
     7801 atgaagaatc acttctgcac gatttctggt tggttcaata taattttgca gatttgtgag
     7861 attgatactg atccagacct gatgggcaaa ggactccact tccccaatcg gcatctgagt
     7921 aaaacgataa tagtagctat cagggtcgtt ttgggctagg ctcagcatct tcaagaaacg
     7981 gtccagatac caactttcaa tatcatctac tccagcatct acatagatgg aaaagtcaaa
     8041 gaagtcagtg atatagagac gatcgttttg tggattttga aagacattaa ttccctcaac
     8101 aattacaaaa tcagcagctt tgacactttg tttcttttcg ggtacgatgt cgtaaacttc
     8161 atgagaatag acaggaatat ctacatcttg tccatttttg atgcggtcca agaagttgag
     8221 aagagcttcc atatcatagc tttcaggaaa tcctttacga tttaaaatcc cctgctcaat
     8281 caaggtttga ttgggataga gaaaaccatc agttgtaacc aactcaaccg tagcatctgt
     8341 aaacgtacgg gacagtagga tttgaagtag gcgactggtt gtggattttc caacggcaac
     8401 actcccagaa accccaataa taaaaggttg agatttactt tcacgttgga ggaaaattcc
     8461 ttttgaaaag gctaaatctt ccttagttcg cttgtaaatc tgaatcagat gagccaaggg
     8521 gagatagata tctgtaacgt cttggagact gatttggtca ttaaaactct tgatagattc
     8581 caattcttct tctgtcaaag gaggtgttgt ctttcgatgt aaagattgcc aagtctggcg
     8641 gctgattttt tcaaaatgta aaaattcgtt ggtcatttgt tttcccctag atattttgtc
     8701 tatcatacca taaaaagcta aaaaaataaa gcggtttctt gaatgtggta agtcaatggt
     8761 atatagtata agtgatcaat ttattgatga aataggagat agattctact gatttataag
     8821 cttataaagg gggcttttta gttcacaact tagtaggagg tgtactatga aaatcttaaa
     8881 acgttatata ttggaactct gttttatttt aagttttgct ttacctttta taaaaggaac
     8941 caatgcagat aatggtagat gctttgtgga aacctattac ggttttactt ttttgatgga
     9001 acatgctatt gtaacagctg tctttatctg ttcgttctta attgctttct tactaaaaaa
     9061 cgatggacga aatggattgc tgcgggtagt tattgctttt tagtcctacg gattgcgaca
     9121 gagggttatt tcttccacat gtcactagaa gatttgatac gactttggac aagtttggaa
     9181 atcctgacac aaacgtatca gttgggcttt tatctaaaca ttttgttagg aattttgttg
     9241 ataataaagt attttaaggt taagcaatag aaataataat ctgcctgatt atagatgata
     9301 aactttttgt ttttatctgg actgtgatat ttatagttct aaatggttta ttatttataa
     9361 gtgttgaaag agattgaaga aaatggcttc gtgacttgct ttcttcggtc tttttcgttt
     9421 tattatcaat ttgtaaacga tttacaattt gagtcaaatt atggtaaaat agtgtcatga
     9481 gtaaaatgta ctatgcagaa aatcctgacg ctgctcacga cattcatgag ttgagagtgg
     9541 acttgttggg agaaaaaatg acttttttga cagatgcggg tgtttttagc aagaaaatgg
     9601 ttgactttgg aagtcaactc ttgctcaagt gtctggatgt caaccaagga gagacagtcc
     9661 tagatgtagg ctgtggttat ggtccattag gtttgtcctt ggtcaaggtt tacggagttc
     9721 aggcgaccat ggttgatatt aacactcgtg ccctggattt agctcagaga aatgctgaaa
     9781 aaaataatgc aaaagcgacg atatttcaat ctaatattta tgcaagagtt caagggcgat
     9841 ttgaccatgt catttccaat cctcctatcc gtgcgggcaa gcaagtcgtt catgaaatca
     9901 tcgagaaaag taaagatttc ttggaaattg gtggagattt gaccatcgtc atccagaaaa
     9961 aacaaggagc tccaagtgcc aaatctaaga tggaagatgt gtttggcaat tgtgaaatcc
    10021 ttaaaaaaga caagggatac tatatcctta gaagtgtgaa agaatgagag cagttgattt
    10081 aatccaaaaa aaacgagacg gtcaagaact gacttcaagt gaaattgaat ggctgataga
    10141 aggctatgta tcaggaactg ttcctgatta tcagatgtct gcctttgcta tggctgttta
    10201 ttttaaagga atgacgactc gagaaatctc tgatttaacg atgaatatgg ttaagactgg
    10261 gcaagagttt gacctgtcag ccattgatgg tgtgaaagtt gacaagcatt ctactggtgg
    10321 tgtcggcgat aaggtaacct tgattttagc tcctcttgtt gctagcttcg gtgtgcctgt
    10381 agcaaaaatg agtggtcgtg gtcttggcca tacaggcgga acaattgata aattggagtc
    10441 cattaggggc tatcaagtag aacgtagtca agaggatttt attcgtcagg ttcaggatat
    10501 tggagtatct gtcattgggc aatcagacca gctggtcaaa gcagataagc ttctctatgc
    10561 ccttcgtgat gtgaccgcaa ctgtcgacac gattcctttg attgcgagtt cggtgatgag
    10621 caagaaaatt gcggcagggg cggatgctat tttgctagac gtgactgtcg gtgagggtgc
    10681 cttcatgaag acggttgatg aggcgcgtga gttggctcaa actatggtag atcttggtaa
    10741 ggttgttgga cggaagacag tagcagtgat tacggatatg agccagcctt tggggcgagc
    10801 gattggaaat cgtctggaaa tccttgaagc attggagatt ttgcaaggtc aaggacgtca
    10861 ggatattacc cactttattt gtgaattggc tcaaattatg cttggtttgg caaatgttaa
    10921 caaaactgtt gaagaagttc gccaacatct tgaaaatggt caagcactgg ctaagtttga
    10981 ggaaatggtg caagcccaag gtggagactt ggaagacctc tatcgtcctg taaatgtagc
    11041 tcatgtggtg gaaatccctg ctcaggaaac gggtgtcatt tcagctcttc cagctatgga
    11101 ctttggtctt tatgccatga ggctgggtgc tggtcgtgca gttaagtctg atgtcttgga
    11161 ctatgaaaca ggaattgttt ttgaaaagaa gattggagac tccgttcaaa agggtgaaat
    11221 tgttgcaaaa gtatacacaa atgaaaaaat tccacctcaa ctagttacag atttccaaaa
    11281 atgtgttaaa ataagtgatg aagtgaaaaa aatccgtgaa attgtagaaa tcatctccta
    11341 aatggaggga aaaccgaaat gaaattaaat aaatatatag atcatacgct tttaaaacaa
    11401 gatgcaaaga aaaaacaaat tgatagtttg ttgtctgagg ctagagagta tggctttgcc
    11461 agtgtttgcg ttaatccgac ctgggttgaa catgctaaaa aaggacttga aggcacagat
    11521 gttaaggttt gcacagtagt aggtttcccc ttgggagcaa caacttcaac tgtgaaagct
    11581 tttgaaacaa aagaagctat ccaaaatggg gctgatgaga ttgacatggt aatcaatgtt
    11641 ggggctctca agtcaggtga tttggccttg gttgagtcgg atattcgtgc agtagtggaa
    11701 gcaagtgggg acaagttagt gaaagttatt attgaagctt gccttctgac agaccaagaa
    11761 aaagttttgg cttgccaatt agcccaaaaa gcaggagctg actttgtcaa aacatctact
    11821 ggcttttcaa ctggtggtgc tacgatagca gatgttagat taatgcgtga aacagttgga
    11881 cctgatatgg gtgtcaaggc cgccggtgga gctcgttctt atgcagatgc tcttaccttt
    11941 gtcgaagcag gtgcgacccg tatcggaacg tcagctgggg tagctatttt aaaaggagaa
    12001 ttggcagatg gcgactactg agttgattga actggcaatt gaaaccagca aacatgccta
    12061 tgtcccctat tctcactttc ctatcggagc agttttagta gccaaagacg gaagtgttta
    12121 cacgggagtg aatatcgaaa atgctagcta tcctttgacc aattgcggtg agagaacagc
    12181 tatttttaag gctatatctg aaggccaaag agaattttca gaattgattg tctatggtca
    12241 aactgaaaaa ccgatttcac catgtggtgc ttgtcgccaa gtaatggtcg aattttttga
    12301 acaagattta aaagtgaccc tagtcgcaaa agataaatcg acggtcgaga tgacggtcgg
    12361 ggagttactt ccatactctt ttacagactt aaattagtct gagtcgctct ttgagtggca
    12421 agggtctttg tgaccaatca atccatactt gcaacatcgt tgcacatctt atttaggagg
    12481 ttcagtaatg aacaagaaac aatggctagg tcttggccta gttgcagtgg cagcagttgg
    12541 acttgctgca tgtggtaacc gctcttctcg taacgcagct tcatcttctg atgtgaagac
    12601 aaaagcagca atcgtcactg atactggtgg tgttgatgac aaatcattca accaatcagc
    12661 ttgggaaggt ttgcaagact ggggtaaaga acacaatctt tcaaaagata acggtttcac
    12721 ttacttccaa tcaacaagtg aagctgacta cgctaacaac ttgcaacaag cggctggaag
    12781 ttacaaccta atctttggtg ttggttttgc ccttcacaat gcagttgaag aagcagctaa
    12841 agaacacact gacttgaact atgtcttgat tgatgatgtg attaaagacc aaaaaaatgt
    12901 tgcgagcgta actttcgctg ataatgagtc aggttacctt gcaggtgtgg ctgcagcaaa
    12961 aacaactaag acaaaacaag ttggttttgt aggtggtatc gaatctgaag ttatctctcg
    13021 ttttgaggca ggattcaagg ctggtgttgc gtcagtagac ccatctatca aagtacaagt
    13081 tgactacgct ggttcatttg gtgatgcggc taaaggtaaa acaattgcag ccgcacaata
    13141 cgcagccggt gcagatattg tttaccaagt agctggtggt acaggtgcag gtgtctttgc
    13201 agaggcaaaa tctctcaacg aaagccgtcc tgaaaatgaa aaagtttggg ttatcggtgt
    13261 tgaccgtgac caagaagcag aaggtaaata cacttctaaa gatggcaaag aatcaaactt
    13321 tgttcttgta tctactttga aacaagttgg tacaactgta aaagatattt ctaacaaggc
    13381 agaaaaagga gaattccctg gcggtcaagt gatcgtttac tcattgaagg ataaaggggt
    13441 tgacttggca gtaacaaacc tttcagaaga aggtaaaaaa gctgtcgaag atgcaaaagc
    13501 taaaatcctt gatggaagcg taaaagttcc tgaaaaataa tggatggaag tcatttctta
    13561 ggaagcggcc aagggccgct tttttaagag ttgagacaaa gtcattttgt ctcagtaaag
    13621 cttgctacta gacaaactag caagttttat tgaaataaaa taagactctg aaaggaagag
    13681 cacatggcac acgaaaatgt cattgagatg cgtgatatta ccaaggtgtt tggtggattt
    13741 gttgccaacg acaaaatcaa cttgcaccta cgaaaaggtg aaattcatgc acttttagga
    13801 gaaaatgggg ctggtaagtc cacgctaatg aacatgttag caggccttct tgaaccaacc
    13861 agtggtgaaa tcgcggtcaa cggtcaagtt gtcaatctcg actccccatc taaagcagct
    13921 agcttgggaa tcgggatggt tcaccagcac tttatgttgg ttgaagcctt cacagtggct
    13981 gaaaacatca ttttaggtag tgaattgact aaaaatggtg tgctagatat cgctggagct
    14041 agcaaagaaa tcaaggctct ttctgaacgt tatggcttag ctgttgaccc ttctgccaag
    14101 gtagcagata tctcagttgg agcccaacaa cgtgtagaaa ttttaaaaac actttatcgg
    14161 ggggctgata tccttatctt tgacgaacca acggctgttt tgactccatc agaaattgat
    14221 gagttgatgg ctattatgaa aaatcttgtc aaagaaggaa aatcaattat cttgattacc
    14281 cacaaattgg atgaaattcg cgcagtttct gaccgtgtta cagttatccg tcgtgggaaa
    14341 tcaattgaaa ccgttgaaat tgcaggggct atcaatgctg atttggcgga aatgatggta
    14401 ggacgttctg tttcctttaa aacagagaag caagcctcta aaccaaaaga agtggttttg
    14461 tctatcaaag atttggtggt caatgaaaac cgtggtgttc cagctgttaa aaatctgtcc
    14521 ttggatgttc gtgctggaga gattgttggt attgcgggga ttgatggaaa tggtcagtct
    14581 gaactgattc aagccattac aggtcttcgt aaggttgaat ctggtagcat tgagctaaaa
    14641 ggagattcaa ttgtaggctt gcacccacgt cagattacag aactaagtgt tgggcacgtt
    14701 ccagaagacc gtcaccgtga tggcttgatt ttggaaatga tgatatctga aaatattgcc
    14761 cttcaaacct actataaaga accacatagt aaaaatggaa ttttgaatta ttcaaatatt
    14821 acttcttatg ctaaaaagct gatggaagag tttgatgttc gcgctgccag tgaattagtt
    14881 cctgcagctg cactctcagg aggaaatcaa caaaaagcaa ttattgctcg tgaaattgat
    14941 cgagatcctg atctccttat cgttagccag ccaactcgtg ggttggatgt cggtgccatt
    15001 gagtatatcc acaaacgctt gattgaagag cgtgataatg gcaaggctgt ccttgttgtc
    15061 agctttgaat tggatgagat tttaaacgtc tcagaccgta ttgccgttat ccacgatggt
    15121 aagattcaag gtattgtatc accagaaaca accaataaac aaggacttgg tgtcttgatg
    15181 gctggtggaa acttgggaaa ggagaagagt gatgtctaaa aaattacaac aaatttcggt
    15241 tcccttgatt tctgtattcc taggaatttt actcggagcc attgtcatgt ggatcttcgg
    15301 ttatgatgct atttggggct acgaagaatt gttctataca gcctttggca gtctgcgtgg
    15361 gattggagaa atcttccgtg ctatgggtcc tctggtcttg attggtcttg gttttgcggt
    15421 tgccagtcga gctggtttct ttaacgtcgg acttcctggt caggctttgg caggttggat
    15481 tctcagtggt tggtttgccc tgtcgcatcc agatatgccc cgtcccttga tgattctagc
    15541 aaccatcgtg attgccttga ttgctggtgg gattgtcgga gcgattccag gtattcttag
    15601 ggcctatcta gggacgtcag aggttattgt aaccatcatg atgaactaca ttgtcttgta
    15661 tgtagggaat gcctttatcc atgctttccc taaagacttc atgcaaagta cagattcgac
    15721 cattcgtgtt ggggctaatg caacctatca gacaccttgg ttagctgagt tgactggtaa
    15781 ctcacggatg aatattggta ttttctttgc catcattgcc gttgcagtta tttggttcat
    15841 gctcaagaaa acaactcttg gttttgaaat ccgtgcagtt ggtcttaatc cacatgcttc
    15901 agaatatgct ggtatttctg ccaagcggac tattatccta tctatgatta tttcaggtgc
    15961 cttggcaggt cttggtggag ctgttgaagg tttgggaacc ttccagaacg tctatgttca
    16021 aggttcgtca ttagctatcg gatttaacgg aatggcggtt agtttgcttg cggccaactc
    16081 accaattggt atactctttg cagccttcct atttggcgtt ctccaagttg gggctcctgg
    16141 tatgaatgcg gcgcaggtac catctgagct cgtcagcatt gtaacagcgt ctattatctt
    16201 ctttgtcagt gttcattacc ttatcgaacg ctttgtcaaa ccgaaaaaac aagttaaagg
    16261 aggtaagtaa agatgtctat tataaccttg ctccccctct tggtgtcttc tatgctgatt
    16321 tactcagcac ctctcatctt tacaagtatc ggtggtgttt tctctgaacg tggtggtgtg
    16381 gtaaacgttg gccttgaagg aattatggtt atgggtgcct tttctggagt tgtctttaac
    16441 cttgaatttg cagaacaatt tggagcagca actccatggc tatccttgct tgtagcagga
    16501 ttggttggta gtgttttctc tatcatccac gcagcagcga cggttcattt ccgtgcagac
    16561 catgttgtca gcggtacggt attgaacttg atggcgcctg ccttggctgt tttcttagtt
    16621 aaagttcttt ataacaaagg acaaaccgac aacctaagtc aaacttttgg acgctttgat
    16681 ttcccagtct tggcaaatat cccagtgatt ggtgatatct tcttcaagtc aactagtcta
    16741 cttggttatc tggcgattgc cttctcattc cttgcttggt ttattctctt taagactcaa
    16801 tttggtcttc gtctccgctc tgtcggtgaa catcctcaag cagcggatac cttgggaatc
    16861 aacgtctaca agatgagata tttaggggtt attatttcag gttttctagg tggaattggc
    16921 ggagcgattt atgcgcaatc aatctcagtt aacttctcag tgacaactat tgttggacct
    16981 ggatttatcg cccttgctgc gatgatcttt gggaaatgga atccaatcgg tgctatgctt
    17041 tctagtctct tctttggact ttcacaaagt ttggctgtta tcggttctca attgccgttc
    17101 ctacaaggag tgccagcggt ttatcttcaa attgcacctt atgttttgac aattcttgtc
    17161 ttagcagcct tctttggaaa agcagtcgca ccaaaagcag atggtatcaa ctacatcaaa
    17221 tcaaaataag catacaaaaa aacgtcagtc caatgactga actgcccccc aaaagttaga
    17281 cagaaaaaat ctaacttttg gggtgttttt attatgaaa
//
LOCUS       DAVLVF010000031        17243 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVLVF010000031 DAVLVF010000000
VERSION     DAVLVF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17243
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.031"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..1076
                     /locus_tag="VYN66_001076"
                     /pseudo
     CDS             1..1076
                     /locus_tag="VYN66_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1175..2797)
                     /gene="groL"
                     /locus_tag="VYN66_001077"
     CDS             complement(1175..2797)
                     /gene="groL"
                     /locus_tag="VYN66_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV1495112.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2813..3097)
                     /gene="groES"
                     /locus_tag="VYN66_001078"
     CDS             complement(2813..3097)
                     /gene="groES"
                     /locus_tag="VYN66_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV1495113.1"
                     /translation="MLKPLGDRLVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3253..3648)
                     /locus_tag="VYN66_001079"
     CDS             complement(3253..3648)
                     /locus_tag="VYN66_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1495114.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EVDFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3726..4487)
                     /locus_tag="VYN66_001080"
     CDS             complement(3726..4487)
                     /locus_tag="VYN66_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1495115.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(4529..5155)
                     /locus_tag="VYN66_001081"
     CDS             complement(4529..5155)
                     /locus_tag="VYN66_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV1495116.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFETAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDSAKHWTA"
     gene            complement(5171..5488)
                     /locus_tag="VYN66_001082"
     CDS             complement(5171..5488)
                     /locus_tag="VYN66_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV1495117.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(5485..5784)
                     /locus_tag="VYN66_001083"
     CDS             complement(5485..5784)
                     /locus_tag="VYN66_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV1495118.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(6069..6260)
                     /locus_tag="VYN66_001084"
     CDS             complement(6069..6260)
                     /locus_tag="VYN66_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV1495119.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(6421..7095)
                     /locus_tag="VYN66_001085"
     CDS             complement(6421..7095)
                     /locus_tag="VYN66_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495120.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(7101..7547)
                     /locus_tag="VYN66_001086"
     CDS             complement(7101..7547)
                     /locus_tag="VYN66_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1495121.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPCYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7661..8164)
                     /locus_tag="VYN66_001087"
     CDS             complement(7661..8164)
                     /locus_tag="VYN66_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1495122.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVTLSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(8161..8523)
                     /locus_tag="VYN66_001088"
     CDS             complement(8161..8523)
                     /locus_tag="VYN66_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEV1495123.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8589..10448)
                     /locus_tag="VYN66_001089"
                     /pseudo
     CDS             complement(8589..10448)
                     /locus_tag="VYN66_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10487..10936)
                     /locus_tag="VYN66_001090"
                     /pseudo
     CDS             complement(10487..10936)
                     /locus_tag="VYN66_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(11060..11776)
                     /locus_tag="VYN66_001091"
     CDS             complement(11060..11776)
                     /locus_tag="VYN66_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1495124.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12657..14072)
                     /gene="ply"
                     /locus_tag="VYN66_001092"
     CDS             complement(12657..14072)
                     /gene="ply"
                     /locus_tag="VYN66_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV1495125.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(14083..14415)
                     /locus_tag="VYN66_001093"
     CDS             complement(14083..14415)
                     /locus_tag="VYN66_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495126.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTGIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(14486..15094)
                     /locus_tag="VYN66_001094"
     CDS             complement(14486..15094)
                     /locus_tag="VYN66_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495127.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(15107..15565)
                     /locus_tag="VYN66_001095"
     CDS             complement(15107..15565)
                     /locus_tag="VYN66_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV1495128.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15690..16089
                     /locus_tag="VYN66_001096"
                     /pseudo
     CDS             15690..16089
                     /locus_tag="VYN66_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16854..17114)
                     /locus_tag="VYN66_001097"
     CDS             complement(16854..17114)
                     /locus_tag="VYN66_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495129.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFFDG"
BASE COUNT         5104 a         3912 c         3089 g         5138 t
ORIGIN      
        1 cgtcgattca agtgttatca ctgttcaaaa atgatggtcg ctgaaacttc tatcgtcaag
       61 aagaatcacc aaatccctcg tattatcaac caaaaaattg cgcaaaagtt aattgaaaag
      121 atttctatga ccgatattgc ccatcagctt tccatctcaa cttcaactgt tattcgtaag
      181 ctcaatgatt ttcactttaa acatgatttt tcttgtcttc ctgagattat gtcttgggat
      241 gagtatgctt ttacaaaggg aaagatgagt ttcattgcgc aagattttga taatcttaat
      301 attatcactg ttcttgaagg cagaacacaa gctgtcatcc gaaatcactt tcttcgctac
      361 gatagagccg tccgatgtcg cgtcaaaatc attactatgg atatgtttag cccttactat
      421 gacttggcta aacagcttcg ctttcaaatt tctaggctca ggctgaaaca gtctcccaga
      481 ctgtttcact cccgaatgct aaaatcgttc ttgatcgctt tcacattgta caacatctta
      541 gccgtgctat gagtcgtgtg catgtccaaa tcatgaatca gtttcatcga aaatcccatg
      601 aatacaaggc tatcaagcgc tactggaaac tcattcaaca ggatagccgt aaactgagtg
      661 ataagcgatt ttatcgtcct acttttcgta tgcacttaac caataaagag attttaaaca
      721 agcttttgag ctattcggaa gacttgaaac accactatca gctctatcag ctcttgcttt
      781 ttcacttcca gaacaaggaa ccagagaaat ttttcggact tattgaggac aatcttaagc
      841 aggttcatcc tctttttcag actgtcttta aaaccttcct caaagataaa gaaaagattg
      901 tcaacgccct tcaactacac tattctaatg ccaaactgga agcgaccaat aatctcatca
      961 aacttatcaa gcgcaatgcc tttggttttc ggaactttga aaacttcaaa aaacggattt
     1021 ttatcgccct gaatatcaaa aaagaaagga cgaaatttgt cctttctcga gcttagctga
     1081 cttcaaccca ctacagttga caaagagcct ataaaaagaa gggttagtca ttccctcctt
     1141 ttgtgttttt tataagttgt tttctataga aagcttacat catcccgccc atcatacttg
     1201 gatccattgc tggagctggg gctactggtt ctggtttatt ggctacgact gcttctgttg
     1261 tcaaaatcaa gctggctaca gatgctgcat tttgtagggc tgaacgactc actttaactg
     1321 gatcaatgat accttgatca atcatgttaa cccactcgcc agttgctgcg ttaaatccta
     1381 taccaagctc agcatttttc aaacgatcga taacgataga tccttcaaat cctgcattgt
     1441 gagcaatttg acgaacgggt tcttccaaag cacggagaac aatattacgt cctgttgctt
     1501 catctcctgt caattccaag gtagcaacag ctggaatcac attggcaaga gctgttccac
     1561 cgcctgcaac aataccttct tcaacagctg cacgagtagc gttgagggca tcttcaatgc
     1621 ggagtttcat ttctttcaac tcagtttcag ttgcggctcc gaccttaata accgctacac
     1681 cacctgacaa tttggccaag cgttcttgca atttttcacg gtcaaattca gaagttgtag
     1741 tttcgatttg agacttgata accgcaacac ggtgagaaat cgcttcagga tttcctgcac
     1801 cttctacaat aaccgtgcta tctttgtcca cggtcactct cgctgcttga ccaagagctt
     1861 caattgtcgc atctttcaac tcaagaccaa ggtcttctgt gataactgtt ccgcctgtta
     1921 agatggcgat atcttcaagc atggctttgc gacggtcacc aaaaccaggt gccttgactg
     1981 ctactacgtt gaaggttcca cgaatcttgt tcaaaacaag agttggaaga gcctcgccat
     2041 ccacatcatc cgcaataatc aagagtggac gattgctttg gagaatgctt tccaaaagtg
     2101 gcaagatctc ttggatattg gaaattttct tgtctgtaat caaaatgtac ggattttcaa
     2161 ggtcagccac cattttttcg ctatctgtca ccatgtactg tgaaaggtaa ccacggtcaa
     2221 actgcattcc ttctacgact tcaagctctg tttccatacc acgtgactct tcgatggtga
     2281 tgacaccgtc tttgccaact ttttccattg cttcagagat gtactcacca actttttcag
     2341 aacgagaaga tacggctgca acttgagcga tagcttcttt attggcaaca gggatggcgt
     2401 tgtttttcaa agcttcaact gctgcggcaa ctgctgtttc aatcccacga cgaataccga
     2461 ttggatttgc acctgctgtg acgtttttga ttccttcacg gacgatagct tgggtcaaga
     2521 ctgttgcagt cgtagtcccg tcacctgcga tatcattggt tttagaagct acttctgata
     2581 ccaatttggc acccatattt tcaaaatggt cttctaattc aatttctttg gcaatagtca
     2641 caccgtcatt ggtaatcaat ggtgagccaa atgatttttc aagaacgaca ttgcgacctt
     2701 ttggtcccaa ggttacttta acagtatctg caaggatatc gacaccacgg accatagctg
     2761 aacgggcatc tgatgaaaat ttaatttctt ttgacatact tactttctcc ttctattctt
     2821 caatgattgc caagatgtta gcttcgccta cgatgatata cttttcatcg ccatctttga
     2881 catcaagacc tgcgtgggct tcaactaaga cacggtctcc agttttaaca cttggagcaa
     2941 ccaagtcacc gttcaaggta cgaacacctt gtccagtagc cacaacttgg gctgttttgg
     3001 ttttttcttg ggctgaacct gcgagaacaa agcctccaac agtttgttct ttttcttcta
     3061 cttttaagac caaacggtcc cctaatggtt tcaacatctg atttcctcca taatgagata
     3121 gatattatta gcactcttta tacctgagtg ctaattcata gttctattgt atcacttagt
     3181 cagaaataat caagaaaaaa gtctgacttt ctcaagataa aaagcctgag accaactcag
     3241 actttttaat tcttaaaatg gcaattcttc ctcttccaag accaaatctg ccaaatcttg
     3301 gcctgcatta ttttcacgca tagcacgttg ggcacgactt tccaagagtt ggaatcctgt
     3361 cacaaggact tcggttacat aattcatctg accatttttc tcaaagcgac gggtacgcaa
     3421 ttctccatca acggaaatga gactaccttt ggttgcgtag cttgccaaag tttctgctag
     3481 tctgccccat aggaccatat tgacaaaatc aacttcacgt tcaccgtttt ggtctttgta
     3541 acgacggttg acagcgatag ttgctcgcgc tactgacttg tcattgttgg ttttgtgcaa
     3601 ttctggtgta gacgttaaac gtccaatcaa gataacttta ttatacatat tttcttcctc
     3661 ctacttatct attcgtagga aatcaaaaaa agttacagaa atttgtaact tttcgagaaa
     3721 attttttatt ttttatgaac catgaaacct gtcgcctgtt gattggccat aatggtcata
     3781 tctgtaatct gaacacgacg aggttgacta gtcacataga ctactgtatc tgcaatatcc
     3841 tgagcttgca aagcttctat tccttggtaa acggacgcag ctcgttcttt atcaccatga
     3901 aaacgcactg tagaaaaatc tgtttcgaca attccaggct gaatggttgt caccttgata
     3961 tccgttgcga tggtatcaat tcgcagtcca tccgaaaagg tcttaaccgc agccttggta
     4021 gctgagtaaa cagcggcacc agcataggcg tagattcctg cggttgaccc catattgata
     4081 atatgacctt gattgacttt tatcattgct ggcaagaaac agcgagtaac cgccatcaga
     4141 cctttaacat tggtatccaa catggtcaac atatccaact cctcatagtc ttgataggga
     4201 gccaagtcaa gagccagtcc ggcgttgttg accaagatat caatcctccc tatcgtttct
     4261 agaatatcag agcaaacagt cttcaccatg accatatccg tcacatctag tggaaaagtc
     4321 cagacggttt gatttggaaa gcttactgca aactccgact tgaggatttc taatctgtct
     4381 atccgtcgtc ctgttagaac gacatcctca ccctgctcca gataagcacg cgcaatcgct
     4441 tcaccgattc ctgaggttgc tcccgtaatc acaacatttt ttgccatctt atttccttct
     4501 agctggtcta tcagatatta acaacttctt aggcagtcca gtgtttagct gagtcaaatg
     4561 gtgttccgac aacttggtct tctgataatt caagcacccc acgtttttgt ggagcatttg
     4621 gcagatgcaa ttcacgagga ctgcacatca taccaaaact cttttcacca cgaagttcac
     4681 ctgggaaaat gagattccct tttggcatca tagctccagg aagcgcgaca atggttttca
     4741 accccacacg cgcattggga gctcctgcaa cgatttgtac agtcttatca cttgcgactg
     4801 caacttggca gatgttgagg tggtcactat ctggatgggc taccatctca acaatttcac
     4861 cgacaacaaa cttaggttcc ttatcattaa caatttcttc tgtaaaacct tccgcctgca
     4921 actcttggtt caaacgagcg acttgctcat ctgtcaaaaa gacttgaccg cgctctgcag
     4981 tttcaaataa acttgaaact tcgaaaatat tccaagccac tgtttcccca ttttctttga
     5041 ggaaaacacg ggctaccttg cctttgcgtt ccacattcag tttggcatct ccgctatttt
     5101 tcacgatgac cataaggaca tcaccgacat gttctttgtt atacgtaaaa atcattgttt
     5161 cctttttctc ctatttcaat cctgctaaaa aatcgttaat ttgctcctta cttttacggt
     5221 cgcgattgac aaaacgaccg atttccttgt ccttttctag aacaacaagg ctaggaattc
     5281 cgtaaacatc ccagagtttg gccaaatcca tatactgatc tcggtccatt cgaataaagg
     5341 tgaactctgg attggtctct tcaatctctg gtaaggcagg ataaatataa cgacaatcgc
     5401 cacaccagtc tgccacaaaa aggaagacct tcttgcccgc tttttccact aaagatgcta
     5461 attcttctaa acttgctggc tgtatcataa gacttcctcc tcatagacta ggtcttcatt
     5521 ttcatagaca aaggtataat gacggccatc ctcaaaaatg acgccaccaa ccaagctctc
     5581 cagactgctt tcgtaaactt gaacataaag ggtcgcaatt tcccccatgt cggaaaaatg
     5641 gtctcgcaca atctctgtca actcttcctg agtcttcatg agcttacggt catctgcaac
     5701 ttttttcgta gcaagggcaa ggcttccgat acctagcaga gccaagcctg ccatccacat
     5761 ttttttagct ttcataccat tcattttaac acaaaaaagg cttcaggaca aatgaggaag
     5821 cagcagaaaa gcaagtaaaa agcctcttcc tttaaggaaa aggacttctt atactaagag
     5881 caaactagaa agctagccgc aggctgctca aaacacggtt ttgaggttgt agataaggcg
     5941 acgttgacgt ggtttgaaga gattttcaaa gagtattatt cttattgcca ggcacctaag
     6001 ttgccaacgt agtaaccatc aggtgtgtag gtattgcgaa gcatcttacc tgatgaagcc
     6061 agataatact acttgccatt gtctttgacc caatcattag caatcatgga accagaagaa
     6121 cttacataat accattctcc cttgtcataa acccaagtac tgactttcat ggttcctgag
     6181 caattaaagg caaaaaaact gtccaatatc attcgttttt taaaagcatt cgacactaca
     6241 tcatctacgc tgtagtacat agatacatca aacgtaaaag ccaaaatatc gaaaaatata
     6301 caacttttaa aggcagcatt tcatttttta aagtctaaca accatagaac tgaaatcaga
     6361 gcaaggcatc aatttcctac tctatagtac gcctattttg atggttgaag tgaagatttt
     6421 ttatttaacg tagacaattt ccaacttccc aaaagccaca tctccacgga taatcaaggt
     6481 tttgctggtt ggggctacag gagcgtcagc cttagctgca ccaaaggagg tttctacttt
     6541 caaatctaca cgccagtgtt gtggaacata gacggttgca tttccgaagg ctacttcaat
     6601 atttaaagtt gcaaaatcac ctagcatctc tgcattatca tagtagattt tagcatcccc
     6661 aaaagcgact tccacttggt catctacgag atcttgatct tgcttataga aggtcccgct
     6721 accaaaagcg acttccttat ccgtgacgac tgtttttttc ccattgtacc accatttttt
     6781 ttcattccag aacttacttg aatgcgtcag ataaccaaca ccaagtacca ccaagatgct
     6841 agcccaaata agagaatgat tggtaactgg taacaagtca taagcgtaat ttgcaatgat
     6901 gagcgccagt aaacctgtaa aaactgccga agtgagatga cgtctaagga tggactcaat
     6961 ggacttataa gcaaaaaaaa caatacctag taaaggccat attttaccat ccaaagaagg
     7021 aattccaaaa ttcccttgca gcaagatcca agctgctaaa accaataaaa caataccaaa
     7081 tgctttcttt ttcatgtttt ttacctcatg tttcttagat tttcttttag gagggattgg
     7141 taatgccgtg agacatgaac ctccttgtgc gtctgataaa aggaaatggt gcccgttcct
     7201 gaaaaggact tgtccactga gtaaatctga cggatgtttg cgatcgttga cttggaaact
     7261 cgattaaaat agcaaggcaa gatagactcc aactcataga gcttgaggcg aacctcataa
     7321 gcttccttct ggttatgagc gtagatcttg ctcccttctg tttcaaagaa gagaatttct
     7381 gacaggtcta gataatattc acctgtcccc ttgtaaaaag tcaacctcgg agcctttgac
     7441 tcttgcaaga gccgttgcaa gtctgcaatt tcatctgtca aagtgggggt cttgatgaca
     7501 atttcagtct cctctaaatt gccgtcaatc tcaattcgta acttcatcac atctcctttc
     7561 tgactctact atatcaaagc taagttagga atacaagggc aaaaaagcaa gtggtcaatt
     7621 tgaagcagta agtggttgta ggactagctt aacttacatg tcagaatagg aagaaaagga
     7681 atccccacac caaaccacaa gcatagccaa ccaagacatc cttgggataa tgaacacccc
     7741 ctaaaaccct caccaacccc agcaaagctg aaaagatcaa gcatactaag ccaataggta
     7801 gactagcatg cagacaggcc atggagatga tagttgccga aaagacatgg cgactgggca
     7861 tcgaccgtcc cgaactatcc ttatcaagaa ggggacagat atcccaagtt tcataaggtc
     7921 tcgggtgatt gattttctta cgaaacaggg acaaggtcac aaaacctgat gcaggaataa
     7981 gcacataaac cccaacttgc tgaccaagtc ctagttgcag gtaagtggtg actagcaaga
     8041 tcagatagac caggggcatg gcgactgtca tcaaacgatt gaaactgcgt aataggaata
     8101 aaagaattgg acgactagtt agtctggagc tgatatttcg ataccattct tgataatttt
     8161 tcatttatgc ttctcatact tttctagaac ttgttgagat gttttcccct tggcaagcca
     8221 atccactagc ttgtccaagc gacgtatttc ctgcatcaga ggatcatcaa tttcctcaat
     8281 acggacaccg caaatcttcc ctgtaattgc aattcgttca gggacataat atggtgcttg
     8341 tctaaaaaaa tctccgtaag taacatccct atccagacaa gcaagaacat cactcacttc
     8401 ataccctgtc aaccaactgg tcacttggtg aactgactct gcctttccac cctttcgctc
     8461 aacttttgca atcaaggcta agtagacagc agcaaatttc atattgtata atttctgact
     8521 catacttctc tctttctatg tgtactagta taaataagaa aaagaaggcc gtcaagcctt
     8581 cttttgattt attcttctgc ttcatcttct gtaaattgac tattgtacaa gtcagcgtag
     8641 aagccacctt gcgccatcag ttcctcatag ttgccttgct cgatgatatt tccatctttc
     8701 atgaccaaga tcaagtctgc atttcggatg gttgacaagc ggtgggcaat gacaaaggat
     8761 gtgcgtcatt ccatcaaacg gtccatggct ttttggatca attcctctgt ccgtgtgtca
     8821 acagaagaag tcgcctcatc caaaatcaaa agcggtgcat ccttaagaag ggcacgagca
     8881 atagtcaata gttgtttttg tcctacagac aaggtcacgg tgtcatccaa gatggtatca
     8941 tagccatctg gcaaggtcat aataaagtgg tgaattccca cagccttact agcttccatc
     9001 attcgttcat cactaatccc tatttgatta tagatgagat tgtctcgaat agttccttca
     9061 aagagccagg tatcctgcaa gaccattgaa aaggcatcat gcacttctga acgcgtcata
     9121 gccttggtat ccacaccatc aatgtgaata cttcccttat caatctcata gaatttcatc
     9181 aaaagattga caatggttgt cttaccagtc ccagtcggcc caacaatggc aaccttttga
     9241 ccagcatgag ctgtcgcaga gaagtcatag tcttgaacat tgacaccgtc caccagaatt
     9301 tctcctgctg acacgtcgta gaaacgtgga atcagattga ccagagttga tttaccagaa
     9361 cctgttgacc caataaaggc cactgtttga ccagtttctg ctttaaagct aacatgttca
     9421 ataactgcct ccgaatttgc cgcatagcgg aaggtcacat ccttaaactc gacctgacct
     9481 ttgaagtttt catcagtcag ctgcacttga acagggtttt ggatagaaga atgcaaatct
     9541 aaaacttgat taatccgctt agcagagacc atagttcggg gaagaacgat gaagagtgct
     9601 cccatgagaa ggaagcccat gacaacctac atggcataag acatgaaaac aatcatgtca
     9661 ctaaagagag gcagacgcgc tatcgaagca gcgtcgttaa tcacataggc cccaatccag
     9721 taaatcgcta cactcaaacc acttgaaatc cccatcatga taggattcaa aatagccata
     9781 agacggttga caaacaaatt caaacgggtc aattcatcat ttactgctgc aaatttttca
     9841 ttttgataat cctctgcatt gtaggcacga acgacacgaa tacctgttaa actctcacga
     9901 gtgatactgt tcagtttatc tgtcaacccc tgaatcaagg actgttttgg aaaggctagc
     9961 gtcatcaaaa cggtcgtcat caggacgttg acaatcacta ccacaagtac ggcccagagt
    10021 cagtattctg aatgacctaa aatcttccca atagcccaga tagccataat tggaccacgc
    10081 gttaccactt gcaagcccat agtaatcaac atttgaactt gagtaatgtc attggtagta
    10141 cgcgtcaaga ggctaggaat tgaaaatttc ttaatctctg tctgcgagta atccaaaact
    10201 cggttaaaaa tatcacttct cagcctacta gtataagaag ccgccactcg ggatgcaaaa
    10261 aatccaactg caactacgga caagaaggca agaaagagca ttcccatcat catgcttgcc
    10321 ggctgccaca actcatctaa attagtttct tgactaccta gcaaatccgt aattttcgag
    10381 atataggtcg gcacttccaa ctctagatag accgaaaagc aagtaaagaa aatggctagt
    10441 aaaatcatcc cccattcttt tctactaatt cttttggtta atttctttat tctctcctcc
    10501 tattcccttg atattttgcc tgtagttgac cgagaacttt ctcaaaaatc agtaattcat
    10561 cttcatcaat gtcttccatc aactgcttgt ctatgcgttc aaaaaaagcc ttaacctgtt
    10621 gcatctgaga acgtgctttg tccgtcagac gaacaaactt agcccgctta tcaacaggac
    10681 tcgcctccaa ttccaccaaa ccattttgca ctatacgctt aaccagatta ctagcaacag
    10741 acttggtaat attgagttcc tgctcgatat ctttaatcaa gaccaagtct tggtttttct
    10801 agcgattatc caaaaaacgc acaacctgac cttgcggccc acccataaat tcaatgccgc
    10861 aacgtttggc ttccttttgc accatcaggt gaatttgatg accaaaacgc ttaaagacta
    10921 acatcggttt atccataatc tcccccttct aaataaaaat agttctctgg agaataatta
    10981 aatttctatg agaactattt tcttgattaa aaaaatccca agtgattttc tcacttagga
    11041 tcatgttcta taggttaaat taaaacccat ctacgttcgt ataaatcttt tggacgtctt
    11101 cgtcgtcttc aagaacgctg taaagttttt caaaggtttc aaggtcttcg cctgacaatt
    11161 ccacttctga ctgaggaatc atttccaatt cagtcacttg gaattcttca ataccagact
    11221 cacggagagc aacgatagcc ttgtgaaggt cagttggcgc tgtgtaaact gtgattgtac
    11281 cttcttgtgc ttctacgtca tccacatcca catccgcttc gagcaattgc tcaaagactg
    11341 cgtctgcatc ttcacctcca aatacaataa cacctttgtt gtcaaagagg taagaaacag
    11401 aacctgaagc gcccatgttt ccgccgtttt taccaaaggc tgcacggaca ttggctgctg
    11461 tacggttgac gttagaagtc aaagtatcca caattagcat agagccattt ggcccaaaac
    11521 cttcgtaacg tccttctgta aaggtttcgt ctgtgtttcc tttggcttta tcaatcgctt
    11581 tatcgataat gtgttttggc acttgggctt gtttagcacg gtcgataacg aatttcaaag
    11641 ctgagtttga ttctggatct ggatcacctt ttttagctgc tacatagatt tctacaccaa
    11701 attttgcata taccttagag ttagctccat ctttagccgt tttcttggct acgatattgg
    11761 cccatttacg tcccattagg aatctccttt tttcacattt taatctttat tattataaca
    11821 caagtttttt tgattttcac tagaggaaat ggattttatt agcaaatcaa gctaggatag
    11881 cactttacct gctaagatgg tcttaccttt ctatctttat caacagacac tcatccacat
    11941 tcaaaaaaca aactagacca ttatctgcaa atagaaagtt tcagccaagt ttgacaaagt
    12001 cagctcaaat tactgtttga agtttgtaga tataagcgac aaaaacaatc atactgcacc
    12061 ttttgttgac agtctactcc agacatatca tagttcaagt aaatactttg aaattcaaca
    12121 gttcttatag gcgctattgt attctaagaa atcaatagaa gagtttctaa gcaaacctct
    12181 aatactcaat aaaaatcaaa gagcaaacca gaaagctagc cgcaggttgc tcaaaacact
    12241 gttttgaggt tgcggatggg gctgacatgg tttgaagaga ttttcgaaga gtataattta
    12301 cgtgttccca agatggagaa gttagactag tacactggca cttctaaacc attgctagca
    12361 attgatttgt tcatatttaa tttcattttt tccataaatg ggtattagat ataaacagca
    12421 aaatatttcc gatacgtgtc gttcttgaat ttccaatcat ctaaaacaag taaaggataa
    12481 tcaatcccct gtatatcaag gaattggcta ccctttttac ttttttacac attctgtttg
    12541 atagattcat tttaacatca cgagcatact ccaatggaaa tcgctaggca agagataaac
    12601 tttcagatat ccgcagagag atcatcgcct ctttttgtcg caagcattct cctctcctag
    12661 tcattttcta ccttatcttc tacctgagga tagagagttg ttccccaaat agaaatcgtc
    12721 cgcttacgca ctagtggcaa atcggttttt tcataaaccg tacgccacca ttcccaggca
    12781 agcccggtac actctctaat tttgacagag agattacgaa cattcccttt taaaggaata
    12841 ctagtggtaa agtgagccgt taaatcctgc ccatttctgt cccaagcctt aggagtcaag
    12901 acttccttac cttgatgatc ataggataat tcattccaag taatataata ttgggcaaca
    12961 taggcaccac tatgatccag cagtaaatct ccgtttctgt aagctgtaac cttagtctca
    13021 acatagtctg tactattttg aaaggtcgca actacattgt cacgtaaaaa agaagttgta
    13081 taggaaatcg gcaagcctgg atgatctgct gtaaagcgac tgccttcttg aatcaagtcc
    13141 tctaccatat ccaccttgcc tgttacaact cgggcacccg aacttgggtc gccccctaaa
    13201 ataaccgcct tcacttctgt attgtccaaa atctgtttcc actctgtctg aggagctacc
    13261 ttgactcctt ttatcaaagc ttcaaaagca gcctctactt catcactctt actcgtggtt
    13321 tccaacttga gatagacttg gcgcccataa gcaacactcg aaatatagac caaaggacgc
    13381 tctgcagaaa ttcctctctg ttttaaatcc tctaccgtta cagtatcttg aaacacatct
    13441 cctggatttt taacagcatc tacgctgact gtataataaa tctgcttaaa attaacaatc
    13501 tgaatctgct tttcgcccga atggacagag ttaaaatcaa tatcaagaga attccctgtc
    13561 ttttcaaagt cagaaccaaa cttgaccttg agttgttcca tgctgtgagc cgtgattttt
    13621 tcatactgca ttctagctgg gacattattg acctgaccat aatcttgatg ccacttagcc
    13681 aacaaatcgt ttaccgctcc gcgaacactt gaattgctgg ggtcttccac ttggagaaag
    13741 ctatcgctac ttgccaaacc aggcaaatca atactataag tcatcggagc acgatcgacc
    13801 gcaagaagag tgggattatt ctctaacaag gtctcatcca ctacgagaag tgctccagga
    13861 tagaggcgac tgtcgttggt agctgttaca gaaatatcac ttgtatttgt cgacaagctc
    13921 cgcttctttc tttcgataac aacaaactca tcgggtagct gattaccctc tttgatgaaa
    13981 cgattttcaa tactttctcc ctgatgggtc aagagtttct ttttatcgta attcatagct
    14041 agtataaagt catttactgc tttatttgcc atcttctacc tcctaataag ttcctggatt
    14101 gagttgcata aactcagact tgttcagcga aatcagccgt ggttggacta agtaatccaa
    14161 aatttcctcg tacaattctt ctgagacatt gcgtcgccgt ctggctagat aagaagtcgg
    14221 aatgcccgta ttatccaaca taaatacctt atctaagtca atcaaggttg gtcttgtaaa
    14281 aggattacga gctagatccg gctcttctat cataaagttc ttgaccaaac gtctggtcaa
    14341 gagagctggt ttgaaggtct gatttttaac caactctttg tttttagtca tgctgttgtc
    14401 aatacagata tacatatgat tcttcacagc caaatcgcta ctaatagtcg gaaaaggcaa
    14461 ataaagagct acaacatctc ctctcttaat caagcaagag cacccccttt tctcctaatg
    14521 taacatagac aggattgacc aagtcttctg attgactcag aatttccaaa gtttgagttt
    14581 ggcgcgctgt caatttagta gcatcttgtc tcttcaatac aaaatgcttg tcgccaataa
    14641 ccttgacaat ataatccttc tccaaagctg actggtaaat ccacatcaga tgttgtctgt
    14701 cctgagaact caagagagaa ggattttcaa gcctcccgat agtctgataa aaatcaaaaa
    14761 caggagctaa ctcctgccaa tctgattggc tagttgtcaa ggctagaaaa agggctttgc
    14821 gagctgatac ttcttggtta gccttgagag ttactttccc ctccaagttt tttagaaatc
    14881 gggaaactcc agaaagcaaa tttttctcta actgcgagaa ataaaaacct ttcgttccca
    14941 gacataagtc tttcatgtcg ctttctctag caaataagag ctcaaacatt tgatagtaaa
    15001 agaaaaatat ctggcactgg gtcgcgctca tcttttcctt atcggcttct ttttttaacc
    15061 agagcaaggg cgacaggtaa ctggattgag acatttcctc tacctcctac tcttttttaa
    15121 ctggagcatc tgcactagct gccacttctt ttgactggat actttcccac tggttaatct
    15181 cctctgagat aagaccttcg catgtcttga caaatagggc aaaagccttg gtctttcctg
    15241 catatttctc cgtttggcat tgatagagga atttttcttt ctccaggagt tgcgcagttt
    15301 tttggtaaga aatccaattt tcctttgcat tatacaaatt gataatcccc tcacacagca
    15361 agccgagact ggataaggca accgaaatca aacggtagcg atcacctggc ataggaatag
    15421 cacaaaagac agctatgagg aaacctgcca cgatttctgt tatttttaat accttatagc
    15481 gcctacgatg ttgaacgctt ttctttaaaa aatgagctat ctgtacgtct aatcgctctg
    15541 tcaggtacat ttcttctggc gtcatattcg taactccttt catttacttt gataaccata
    15601 gtataacata tttgaatatc aaagtatagt aaaaactgga ctataactta ttagacttcc
    15661 tgcgaaacaa aatatggtat agtagttcta tgaattatga agcaagtaaa caactaactg
    15721 atgcacgatt taagcgtctt gttagtgttc agcgtaccac ttttaaagag atgttagctg
    15781 tattaaaaac agcttatcaa aaaagtcgga caagctggta aaatcttggc tgacagtggt
    15841 tatcaagggc tcatgaagat atatcctcaa gcacaaattc cacgtaaatc cagcaaactc
    15901 aagccgctaa cagttgaaga taaagcctgt aaccatgcgc tatctaagga gagaagcaag
    15961 gttgagaaca tctttgtcaa agtaaaaacg tttaaaatgt tttcaacaac ctatcgaaat
    16021 catcgtaaac gcttcggatt acgaatgaat ttgattgctg gtattatcaa tcataaacta
    16081 ggattctagt tttgcaggaa gtctattata tatatgcaca gacatatgaa atctttttga
    16141 tacagccttc tttttacata gagagacagc agttatgata tatttatcca ttcacaaaat
    16201 cttctgtacc aatactacaa taattggtac attttgatta cacaatcgtt tcaaaaaata
    16261 tgatggaaga ctgagtattt tatagtaatt cccttcctat caactagatc aaccagttga
    16321 gtgttgaatg ttgaaacacc ctagcctcag caaaggacta tgatcagcat ttcctagcca
    16381 ataggacgct ccaaatcacc tattctaagt ccatcactag cttggtcagt aagatacaga
    16441 ctgcaaggca agactgacgt ggttgatgag attttggaag agtattacaa tcatagattc
    16501 accagtagac ttgaccgcag atttaactat agtagattga aactagaata gtacacttct
    16561 gcttctaaaa cattgttaga aatcgatttg actgtcctga tcaatttgtc ctgttctcgt
    16621 ttcattttac tatactaaaa aaccacctga aacaaatcaa gtgactagag gataaaaaca
    16681 aagactgggc aaaaagtttt gaaaagcaaa aaacgcataa tatcaggtct tgaaaaacct
    16741 tgctactata cgtttgattg tgggaagatt gacttcattt tctcctgaaa ttgaagtttt
    16801 ttctagtttc caggtataga cgaattcaat atagccatct cgctacaatt attttaacca
    16861 tcgaaaaaat ttttcagaat ttcaaaaatt tcattttgat ccaatgaaat gtcattattt
    16921 tgaagaatag ttgacttcac aggctctaat tctatcacaa agcggtgcat gtctgctttt
    16981 accgcactac ttgatgttat agccgataac ttttcatctc ctagcaagga agctgtcaaa
    17041 cgggtaaggt cattcaaatg ctttttaatg gacttactta aaccttgagc acctgtggca
    17101 gagcgctccc tcatatccag ccaagcttta gccttaaaaa taattaatac tcaatgaaaa
    17161 tcaaagagca aactaggaag ctagttgcag gctgctcaaa acactgtttt gaggttgtag
    17221 atggaagctg acgtggtttg aag
//
LOCUS       DAVLVF010000032        17003 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVLVF010000032 DAVLVF010000000
VERSION     DAVLVF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17003
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.032"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            331..1377
                     /gene="pheS"
                     /locus_tag="VYN66_001098"
     CDS             331..1377
                     /gene="pheS"
                     /locus_tag="VYN66_001098"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV1495130.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1377..1886
                     /locus_tag="VYN66_001099"
     CDS             1377..1886
                     /locus_tag="VYN66_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1495131.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1963..4365
                     /locus_tag="VYN66_001100"
     CDS             1963..4365
                     /locus_tag="VYN66_001100"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV1495132.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISFGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(4433..5434)
                     /locus_tag="VYN66_001101"
     CDS             complement(4433..5434)
                     /locus_tag="VYN66_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1495133.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5590..6036
                     /locus_tag="VYN66_001102"
                     /pseudo
     CDS             5590..6036
                     /locus_tag="VYN66_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6162..6428
                     /locus_tag="VYN66_001103"
     CDS             6162..6428
                     /locus_tag="VYN66_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1495134.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            6689..8938
                     /gene="metE"
                     /locus_tag="VYN66_001104"
     CDS             6689..8938
                     /gene="metE"
                     /locus_tag="VYN66_001104"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV1495135.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            9002..9868
                     /gene="metF"
                     /locus_tag="VYN66_001105"
     CDS             9002..9868
                     /gene="metF"
                     /locus_tag="VYN66_001105"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV1495136.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10553..12766
                     /gene="pnp"
                     /locus_tag="VYN66_001106"
     CDS             10553..12766
                     /gene="pnp"
                     /locus_tag="VYN66_001106"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV1495137.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDLEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDHKPKKEFTETPKDSE"
     gene            12782..13399
                     /gene="cysE"
                     /locus_tag="VYN66_001107"
     CDS             12782..13399
                     /gene="cysE"
                     /locus_tag="VYN66_001107"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV1495138.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13411..14295
                     /locus_tag="VYN66_001108"
     CDS             13411..14295
                     /locus_tag="VYN66_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1495139.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14382..15725
                     /locus_tag="VYN66_001109"
     CDS             14382..15725
                     /locus_tag="VYN66_001109"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV1495140.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15718..16104
                     /locus_tag="VYN66_001110"
     CDS             15718..16104
                     /locus_tag="VYN66_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV1495141.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16108..16992
                     /locus_tag="VYN66_001111"
     CDS             16108..16992
                     /locus_tag="VYN66_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV1495142.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLTSW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
BASE COUNT         5048 a         3323 c         3893 g         4739 t
ORIGIN      
        1 tcatcttatt tcaatccgct ataaagttag ataagaataa gctaacaagc agaagtaaga
       61 taggtcttgc aagaatggta aaaatctgat aaactaagtg gtaattgaat agaaatctga
      121 aagactagta gcccaaggga agtatatcag ggaggagagc cgtgactgca agctctctat
      181 atgaaagctg ggtgaattca cttgcgcatg gatttagaaa tgaaggtctg acttgtcaga
      241 tgaaaacgga tggtaccgcg tgtcaacgct ccgagtggag tttttggcat gtggttttct
      301 ttttatctac gagagactga tggaggaaat atgtcaacta ttgaagaaca attaaaagcg
      361 cttcgcgaag aaacgctgac tagcttgaag cagattactg ctggaaatga aaaagagatg
      421 caagatttgc gtgtctctgt ccttggtaaa aagggttcgc tcactgaaat cctcaaaggg
      481 atgaaagatg tttctgctga gatgcgtcca atcatcggga aacacgtcaa tgaagctcgt
      541 gatgtcttga cagctgcttt tgaagaaaca gctaagctct tggaagaaaa gaaagtcgcg
      601 gctcaactgg ctagcgagag tatcgatgtg acgcttccag gtcgtccagt tgcgactggt
      661 caccgtcacg ttttgacaca aaccagtgaa gaaatcgaag atatcttcat cggtatgggt
      721 tatcaagtcg tggatggttt tgaagtggag caagactact ataactttga acgtatgaac
      781 cttccaaaag accacccagc tcgtgatatg caggatactt tctatatcac tgaagaaatc
      841 ttgctccgta cccacacgtc tccagttcag gcgcgtgcta tggatgccca tgatttttct
      901 aaaggtcctt tgaagatgat ctcgccaggg cgtgtcttcc gtcgcgatac ggacgatgcg
      961 acccacagtc accaattcca ccaaatcgaa ggcttggtag ttgggaaaaa tatctctatg
     1021 gctgatcttc aaggaacgct tcagttgatt gtccaaaaaa tgtttggtga agagcgtcaa
     1081 attcgtttgc gtccatctta cttcccattc acagagccat ctgttgaggt ggatgtttct
     1141 tgcttcaagt gtggtggaga aggctgtaac gtatgtaaga aaacaggttg gatcgaaatt
     1201 atgggagccg gtatggttca cccacgtgtc cttgaaatga gtggtatcga tgcgactgta
     1261 tactctggtt ttgcctttgg tcttggacaa gagcgtgtag ctatgctccg ttatggaatc
     1321 aacgatatcc gtggattcta ccaaggagat gtccgcttct cagaacagtt taaataatga
     1381 ttagaaaagt agaaatggca gatgttgagg tgttggctaa aattgccaaa caagcctttc
     1441 gtgaaacctt tgcgtatgat aatacggaag agcagttaca ggaatacttt gaagaggctt
     1501 atagtctgaa aactttgtca actgagttgg gaaatcctga ctctgaaacc tatttcatta
     1561 tgcatgagga ggagatagct ggttttctca aagtcaactg gggaagtgct caaactgaga
     1621 gagaattaga ggacgctttt gaaattcaac gcctctatgt gctacaaaaa ttccaagggt
     1681 ttggactagg taagcaactg tttgaattcg cacttgaact tgctacaaaa aatagttttt
     1741 cttgggcttg gctaggtgtt tgggagcata atacaaaagc tcaagcgttt tataatcgat
     1801 atggttttga aaaatttagc caacatcatt ttatggttgg tcaaaaagta gatacggatt
     1861 ggttactgag aaagaaatta aggtaagaag aggattcttc tattgaaatg ataggaaagg
     1921 aaaagaacca gtgtggcaat ggtcattctg gagaactaaa ttatgcttat atcttataaa
     1981 tggttaaaag aattggtgga cattgatgtg ccatcacaag agttggctga aaaaatgtca
     2041 actacaggaa tcgaggtaga gggtgtcgaa tcaccagctg ctggtctctc aaaaattgtc
     2101 gtcggtgagg tcttgtcttg cgaagatgtg ccagagactc acctccatgt ttgtcaggtt
     2161 aacgttggcg aagaagagcg tcagatcgtt tgcggtgccc caaatgtgcg tgctgggatc
     2221 aaggtcatgg tggctcttcc aggagctcgt atcgctgata actacaaaat caaaaaagga
     2281 aaaatccgtg gtttggagtc acttggaatg atctgttcac ttggtgaatt gggaatttct
     2341 gactcagttg tgcctaagga attcgcagat ggcatccaaa tcttgcctga agatgccgtg
     2401 ccaggtgagg aagtcttttc ttacctagac ttggatgatg aaatcatcga actttccatc
     2461 acaccaaacc gtgcagatgc cctttctatg tgtggagtgg ctcacgaagt ggcagccatc
     2521 tatgacaagg cagtcaactt taaagaattt actctaacag aaactaatga agctgcggca
     2581 gatgcccttt ctgtcagcat tgagacagac aaggcgcctt actatgcagc tcgtatcttg
     2641 gacaatgtga ccatcgcacc aagtccacaa tggttgcaaa atcttctcat gaacgaagga
     2701 atccgtccca tcaataacgt agtggacgtg accaactaca tcctgctcta ttttggtcaa
     2761 ccaatgcatg cctttgactt ggataacttt gaagggactg acatccgtgt gcgtgaagcg
     2821 cgtgctggtg aaaaattggt gaccttggac ggtgaagaac gtgacttgga cgtgaatgac
     2881 ctagtcatca ctgtcgcaga caagccagta gcccttgcag gtgtcatggg tggtcaagca
     2941 acagaaatct ctgaaaaatc tagtcgtgtt gtccttgaag ctgctgtttt caatggcaaa
     3001 tctatccgta agacaagtgg tcgcctgaac cttcgttctg agtcatcttc tcgctttgaa
     3061 aaaggaatta atgtggcaac agttaatgaa gcccttgatg cggcagctag cctgattgcg
     3121 gaacttgcag gtgcgacggt gcgtaagggc atcgtttcag cgggtgagct tgatacttca
     3181 gatgtagaag tttcttcaac ccttgctgat gttaatcgtg tcctcggaac tgagctgtct
     3241 tatgctgatg tagaagacgt cttccgtcgt cttggctttg gtctttctgg aaatgcagac
     3301 agctttacag tcagagtccc acgtcgtcgt tgggatatca caatcgaagc tgacctcttt
     3361 gaagaaattg ctcgtatcta tggttatgac cgcttgccaa ctagtctacc aaaagacgat
     3421 ggtacagcag gtgaattgac agccacacaa aaactccgcc gtcaagttcg tactattgct
     3481 gaaggagcag gtttgacaga aatcatcacc tatactctaa caactcctga aaaagcagtt
     3541 gagtttacgg ctcaaccaag taaccttacg gaactcatgt ggccaatgac tgtggatcgt
     3601 tcagtcctcc gtcaaaatat gatttcaggt atccttgata ccgttgccta caacgtggct
     3661 cgtaagaata aaaacttggc cctttacgag attggaaaag tctttgaaca aacaggtaat
     3721 ccaaaagaag aacttccaaa tgaaatcaac agttttgcct ttgccttgac aggcttggtt
     3781 gctgaaaaag atttccaaac agcagcagtt ccagttgatt tcttctatgc taagggaatc
     3841 cttgaagccc tatttactcg tttgggactc caagtaacct atacagcaac atctgaaatc
     3901 gctagccttc atccaggtcg tacagccgtg atttcattcg gtgaccaagt tcttggtttc
     3961 cttggccaag tgcatccagt cactgccaag gcttacgata ttccagaaac gtatgtggct
     4021 gagcttaacc tttcagctat cgaagctgcg cttcagccag cgactccatt tgtagaaatc
     4081 accaaattcc cggcagtcag ccgtgacgtt gcccttctcc tcaaggcaga agtgactcat
     4141 caagaagttg tagatgctat ccaagctgcc ggcgtgaaac gtttgacaga tatcaaactc
     4201 tttgacgtct tctcaggtga gaaattggga cttggtatga agtcaatggc ttatagcttg
     4261 accttccaaa atccagaaga tagcttaacg gacgaagaag tcgcacgcta tatggaaaaa
     4321 atccaagcat cgctcgaaga aaaagtcaat gcagaagtgc gttaaacgat agtatgatac
     4381 aaattccccc tctagtttat gaacactaga ggggtgattt tatataaaat aattaaaatg
     4441 tgatttctgt aaaaatacat ctatgatcag agttttgaaa acttacaatg tctaaatctt
     4501 taacatagta gtggttttta ggcaataaaa tatgatctat tgttgcatta aaaagttttg
     4561 gactttggct attccaagtt cctctttcaa aaggtggcag tacatttaat gcgtccctat
     4621 gagagcttat ttttgcaagt gctccatgac gcatagttgc attaaaatca cctgcaataa
     4681 tagcctttgg atattttgaa gccaattgat tatgaatgat gtttaagtct tgcttccaga
     4741 tttccattaa acctggcaga ggaggagcag tatgcaaggc aatgatatct ggtatatttt
     4801 gttttctcga atgtaataca attgtcccga accgtgttgt atgaaaagtt ttagcttctg
     4861 tatagaaacc ataacttttc ttgacaatca cagtcacagg agctattcca ctattggtag
     4921 gtggagaagt gaaaatatca tagttggcca tagaaagtcc aacttgatga aacaatagtt
     4981 tgattctctg gttttcttgc tcacctctga tattggtagc tagttcaggg aatatagcca
     5041 tatcggcgtc aaaatggcta aaaattcgct caatatgttg tgcttctatt tgattagcga
     5101 cgttccaagt tactaatttt aaggttttag tcttatttga tgcagaagaa agggttttat
     5161 ctgttccgaa taatagtaat atgagcgata gtaagagcaa aatgccagag taaaaccgta
     5221 tttgtttatt tttcttgtaa aaagataggg aaaaaaatac gaaacttaaa atagcagcca
     5281 tagttgcagt agctaagggg aaggcataaa tctgtgccaa gataggttgg gtagatagcc
     5341 aaatgttaag attagaagat gggaaaaaag aataagttat aatagggata gaaaagagaa
     5401 tgaccgaaag ggcagagaga aatgttttaa tcattgtgtg agtacctcaa tttgcaatgg
     5461 ttgctcggag ctaatgtgac tttgtgactt tacttgattg atattctttt tcttgtcttc
     5521 ctcatagatt aagtacaacc caagtaaaaa aagtccaatt taagattgaa atgattaaaa
     5581 ggcacagaaa tgcaagacaa ttatactaca aaaggcaaac atttgacaat cgatagccgt
     5641 cgcttaatcg aacgatggaa aaaagaagga aaatcaaata gagaaattgc ctctctactt
     5701 ggaaaagctc ctcaaactat ccatactgaa atcaagtatg ggacagtccg acaatgtctt
     5761 ggaaaagggc gcttcaaaga gatttattct gccgactacg ctcaacagtc ttatgaaaat
     5821 aatcgcaagc actcggtcaa gaaatcaagc ttgactaaga aactaaagga aaagattctc
     5881 cactatcata accaaaaatt ttcgcctgaa atgatggtga tggctaaagg ggttaacgtg
     5941 ggaatttcaa ccatttacta ttggattcat cgtggaaaat tggggttaag caagcaggat
     6001 ctgctttatc ctagaaaagg aaaagcgctt aagaaatagg ctagcaccaa ctttaaaccc
     6061 gctggtcaat ctatcgaatc ataattcgct cattttttta aaaatacata gacgtaaaaa
     6121 aaagagggtt aagtgatgaa gaatagtgct attgggagta attggaagga tgtccatctg
     6181 agctctttag caaggaggaa atccttgaaa gtgatatgcg agtggctatc atgagcgagt
     6241 tgattgaggc taggtatgaa caaggaatca gtcagaaaaa gctggaagaa gtcagtggaa
     6301 taagccagcc tgtcatagct aggatggaga caggagagag cagtcttcag ttggatacgg
     6361 tcttaaaagt tctaaccagt ctaggaaaga cactagcagt cgttccactt gaacagggga
     6421 aaagttgact agaatcagtt atagtctttt ctaataacag gtcatagtca cttataagaa
     6481 ttactaataa catgttttag tattctaatt ggaatatagc tgtaaagtaa gaattacaaa
     6541 ggatttgaga cgtttgcttc aagtcttttt cttttgtccg aaacagatat atagtttcaa
     6601 actatatcaa accctaattt tttacaatta tacagacgct ttcttttctg ttaagatagt
     6661 ttcaacaaca aattttggag gacacatcat gtcaactaca atcatcggtt tccctcgttt
     6721 gggcgaattc cgtgaattaa aatttacaac tgaaaaatac tttagaaaag aaatctcaga
     6781 agaagaactt ttagcagcag caaaagactt gcgtgctaaa cactggaaca ttgtcaaaga
     6841 aaaaggcatc actgaaattc catcaaatga cttttctcac tatgataact tcctagatgc
     6901 ggctttcctt ttcaacgtgg tgcctgcatc cgttcaaaac ttggacttgt ctgaccttga
     6961 gcgctacttc gctttgggac gtggttacca gggagaaaaa ggggacgttc gcgcccttcc
     7021 aatgaagaaa tggttcaaca ctaactacca ctacatcgtt cctaaatttg aaaaagacac
     7081 tcaagtaaaa ttggctggtc acaagatttt cgatgagttc caagaagcaa aagaacttgg
     7141 attgaacact cgtcctgttc ttgtaggtcc attcactttc cttcaattgt cagactttga
     7201 agaaggcgtg aaagcagacg acttcgtaga tagcttagtg gttgcttacc aagaagtttt
     7261 tgctaaattg gctgaacttg gtgcgactcg catccaattg gatgaagcgg ctcttgtaaa
     7321 agacttgaca gctgaagaaa aagctctctt cttgaacctt tacaacaaac tcttggctga
     7381 caaaaaaggt cttgaagtct tgcttcaaac ttatttcggt gatgttcgtg acgtatacgc
     7441 tgaccttgta aacttgccag tagatgctat cggtcttgac tttgttgaag gtaagaaaac
     7501 tcttgaactc gttaaaggtg gcttcccagc tgacaagact ctctatgtag gtattgtcaa
     7561 tggtaaaaac atctggcgca acaactacga aaagagcttg gctgttcttg agcaaatccc
     7621 agctgaaaac atcgttttga caagctcttg ctcacttctt catgtgccat ttacaactgc
     7681 taatgaagaa tttgaaccag cactcttgaa ccactttgcc tttgcagttg aaaaattgga
     7741 tgaaatccgt gacttggatg ctatccgcaa tggtcaaggt tcagaagctc ttgcagccaa
     7801 caaagaactc tttgcgactg agcgtgttgg tgaaaatgct gaacttcgtg cgcgtatcgc
     7861 tggcttgaca gacgcagact acactcgttt gccagccttt gcagaacgtg aagctatcca
     7921 agaagaagct ttcaaacttc cagctcttcc aacaacaacg attggttcat tccctcaaac
     7981 aaaagaagtt cgtgccaaac gtttggctta ccgtaaaggt gaattgtctc aaaaagagta
     8041 cgatgctttc cttgctgaaa cgattgacga atggatcaag tggcaagaag atattgactt
     8101 tgatgtcctt gttcacggtg aatttgagcg taatgacatg gttgagtact tcggtcaaaa
     8161 cttgtcagga tacctcttct ctaaaaatgg atgggtacaa tcatacggta tgcgtggggt
     8221 aaaaccacca atcatctggg gtgatgtcac tcgtcttaac cctatcactg ttaaatggtc
     8281 tagctatgca caaagtcgta caaacaaacc tgttaaaggt atgttgactg gacctgttac
     8341 catccttaac tggtcattcc cacgtgaaga catctctatc aaggattcaa ctcttcaaat
     8401 cgcccttgct atcaaggatg aagtacttga ccttgaagct gctggtgtga aaatcatcca
     8461 aatcgacgag gctgctcttc gtgaaaaatt gccactccgc cgtagcgact ggtacgaaga
     8521 ctaccttgac tgggctattc ctgccttccg cttggtacac tcaacagtag cgccagacac
     8581 acaaatccac actcacatgt gttactcaga atttacagat atcatcccag ctatcgataa
     8641 catggatgca gacgttattt cctttgaagc tagccgttca aaccttgaaa tcttggacga
     8701 actcaaagcg aaaaacttcc aaacagaagt gggacctggg gtttacgata tccactcacc
     8761 tcgtgtacca aatgaaggcg aaatcgacaa cacaatcgaa gccatccttg ctaaagtgcc
     8821 aagcaagaaa gtttggatca accctgactg tggtttgaaa acacgtggta tcccagaaac
     8881 aaaagaaagc ttgatccgcc ttgttgaagc agctaaagct gcgcgtgaga aattgtaaga
     8941 ttggatttct ctccatacag tggttgttca aaaagaaatc aactagaaaa ggttattaca
     9001 tatgtcacgc caaacaccgt cactctcatt tgaagtgttc cctccaaacc cagccgtggg
     9061 taatggtaac attatttcag ccttgcagga tatgcaggag ttggctcccc attttatcag
     9121 tgtaactgcc agcaataata aatttaatat caaggaaacg acggttcgtt tggctgactt
     9181 tatccaaaat gacttggcga ttccgaccat tgcccacttg ccagctattt atctaactaa
     9241 ggacaaggtt gctgaaacta ttgctgactt ggacaaagtt ggggtgcaga aaatcttggc
     9301 tcttcgtggg gatattattc cagacgtaga accacaaaag gatttccgct atgcaaccga
     9361 cttgattgag tttatcaagg aacaagcccc tcactttgat attattggag cttgctatcc
     9421 agaagggcat ccagattcgc caaatcagat ttcagatatt caaaatctta agaagaaagt
     9481 ggatgcaggc tgttcgagcc tcgtaactca gctcttcttt gataatgagc gcttctatga
     9541 ttttcaggac aagtgtattt tggctgggat tgatgttccc attcatgcag gaattatgcc
     9601 aattctgaat cgaaatcagg ctctccgact cttgaagact tgtgagaata tccatcttcc
     9661 acgcaaattt aaagccatct tagacaagta tgagcatgac cctgagtcgc tcagagcagc
     9721 aggacttgcc tatgcagtgg accaaatcgt ggacttggta actcaggatg ttgccggtgt
     9781 gcatctctat acgatgaata atgctgatac agcaaaatac atccatcaag caacccatgc
     9841 cttgtttaat caccagtctc taggataata aaagcaaacc attcttctca ggtgagggga
     9901 atggttcctt tttaatggta aagacctcac tttttaagaa aaatatgata aaataaattc
     9961 tgtacgtact tgatacaaag atgaaggtat taaataatac aatgcattta aactgtaaga
    10021 tttaacgaca cgagtgagtc aaatcgatgt tatttgacga gagagttagt aaagcattta
    10081 gctaatcgcc cgatagcgat tagcgtgaaa gctttagata ctttagtatt aaacctttct
    10141 aatgatgtat cttgaaaact gaacttgcct acaactctgt ggaaaaagat aaatctccct
    10201 aggagcagtc gctccgtcgt tcgatttcct attttccttt gagttgcttg acggcttggt
    10261 atcttgatga cttgaatttt ggtctcattt cttaggaaaa aagataagct tcctagcact
    10321 cgtcgagtgc gacgtcacct tcctattttt ctacagaaat gttcggcaag ccgaaccgtc
    10381 caaaatatct tgtgtaattg aacacggccg aaaagctgtg taaaaaagat aaactgtctt
    10441 gtcttcatcg aagacttcgt cagtttccta tttttacttt gcttttgacg tccttggtat
    10501 cttgatcttt gtaggcaagg cgtataattt catcaatcca aaggggatta aaatggcaaa
    10561 acaagtgttt caaacgactt ttgcgggtcg tgagttaatt gtagaaactg gtcaggttgc
    10621 taagcaagca aatggctctg ttgtcgtacg ttacggtgag tcaactgtct tgactgctgc
    10681 cgttatgtct aagaaaatgg caactgggga tttcttccca ctccaagtca actacgaaga
    10741 aaaaatgtat gcggctggga agtttcctgg tggctttatg aaacgtgaag gacgtccttc
    10801 aacagatgcg accttgacag cgcgtttgat tgaccgtccg attcgtccta tgtttgcgga
    10861 aggtttccgt aatgaagtcc aagtcatcaa tacagtgctt tcttatgatg aaaatgcgtc
    10921 tgcaccaatg gctgctatgt ttggttcatc tttggcactg tctatttcag atattccatt
    10981 tgacggacca attgctgggg tacaagtggg atatgtagat ggccaaatca tcatcaaccc
    11041 aagtcaagaa caagcagagc aatctcttct tgaattgaca gtagctggaa ccaagcacgc
    11101 tatcaacatg gtagagtctg gtgctaaaga attgtcagaa gaaatcatgt tggaagcgct
    11161 ccttaaaggg cacgaagctg tcaaagaatt gattgccttc caagaagaaa tcgttgctgc
    11221 tgtcggtaaa gaaaaagcag aagtggaatt gcttcacgtg gatgctgaat tgcaagctga
    11281 aatcattgca gcctacaaca gtgacctcca aaaggcagtt caagtagaag agaaattggc
    11341 ccgtgaagct gcgactcaag tagtgaaaga ccaagtgact gccgtttacg aagaaaaata
    11401 tgcggaccac gaagaatttg accgtattat gcgtgatgtg gctgaaatct tggaacaaat
    11461 ggaacacgca gaagtgcgac gtttaattac agaagacaag gtgcgtcctg atggtcgtaa
    11521 ggtcgatgaa atccgtcctt tggatgcggt tgtcgacttc cttcctcgtg tgcacggttc
    11581 aggtctcttc actcgtggac aaacgcaagc cctttctgtc ttgaccttgg ctccgatggg
    11641 agaaactcaa atcattgatg gtttggatct ggagtacaag aaacgcttta tgcaccacta
    11701 taacttccct caatattctg taggggaaac aggtcgttac ggtgcgccag gtcgtcgtga
    11761 aatcggtcac ggtgcccttg gtgagcgtgc tcttgctcaa gtcttgccaa gcttggaaga
    11821 attcccatac gctatccgtc tagtagcaga agttttggaa tcaaacggtt cttcatctca
    11881 agcttctatc tgtgcgggaa ctcttgccct tatggctggt ggtgtgccaa tcaaggcgcc
    11941 agtagctggt attgctatgg gacttatctc agatggaaat aactacacag tattgacaga
    12001 tatccaaggt ttggaagatc actttggaga tatggacttc aaggttgcag gtactcgtga
    12061 tgggattaca gcccttcaaa tggatatcaa gattcaaggg attactgcag aaatcttgac
    12121 ggaggctctt gctcaagcca agaaagcgcg ttttgaaatc cttgatgtca ttgaagcaac
    12181 cattccagaa gttcgtccag aattggctcc aactgctccg aaaattgata cgatcaagat
    12241 tgatgtggac aagattaaga ttgtcatcgg taagggtgga gaaaccatcg acaagattat
    12301 cgctgaaaca ggtgttaaga ttgatataga cgaagaagga aatgtgtcta tctactctag
    12361 tgaccaagat gctattaacc gtgccaaaga aattattgct ggtttggttc gtgaagccaa
    12421 agtggatgaa gtttaccgtg ctaaagtcgt tcgtatcgag aaatttggtg cctttgttaa
    12481 cctctttgat aagacagatg cccttgttca tatctctgag atggcttgga ctcgtaccaa
    12541 tcgtgtagag gatttggtag aaatcgggga tgaagttgat gttaaggtta tcaaaattga
    12601 tgaaaaaggc cgtatcgatg cctctatgaa ggctcttcta cctcgtccgc caaaacctga
    12661 gcatgatgaa aaaggtgaaa agtctgagcg ccctcaccgc ccacgtcatc aaaaggatca
    12721 caaacctaag aaagaattta cagaaacacc aaaagattca gaataagaaa aggagaaatg
    12781 tatggggtgg tggcgcgaaa ccattgatat tgtaaaagaa aatgatccag cggcccgcac
    12841 cactttggag gttttgctga cttatccagg tgtcaaggcc ttggcggccc accgtctctc
    12901 gcattttctc tggaagcacg gcttcaaact attagctcgt atgtacagtc agttttggcg
    12961 cttttggact cagattgaga ttcatccagg agcccagatt gattcaggtg tttttattga
    13021 ccatggttct ggtctggtga ttggagagac agcgattgtt gaaaaaggcg ttcttctcta
    13081 tcacggagtg actctcgggg gaacagggaa agactgtggc aaacgccatc cgactgtacg
    13141 aaagggagcc ctcatatcag cccatgccca agttatcggg cctgtggaaa ttggtgaaaa
    13201 tgccaaggtc ggtgctgcag cagttgtcgt agcagatgta cctagtgatg tgacggttgt
    13261 cggtattccg gccaagattg tccgtcttca tggtaagaaa gatgagcctg ttattcacga
    13321 agtcgaagaa aaacgggagt attatgtcaa taaactcgag caggctaaag atgccagtca
    13381 cagatcgtct ggtttgtaga ggatacctat atgattcaag caagaaacaa gttaagccaa
    13441 gaggagctat ctgaggcgaa aaaagtaatt aactgttgcc aaaactatga cggtacctat
    13501 cgcgatccct atctctctaa catgcttaat tttgacccaa acatgcccgc ctttttcctt
    13561 tattatgaaa aaggcaaact tgttggttta ttaactgtct atgcagatga ccaagatgta
    13621 gaagtgacga tattggttca tccaggtcat cgccgtcagg ggattgcgcg tgcattgttt
    13681 actagttttg agagagaaac agcttctttc cccattcggt cagtcacttt tcagacagaa
    13741 cgtatttttt tagagaatca tcctgacttt gccagtaact ggggactaat cgaggatgaa
    13801 gagacagaaa cttggttagg taaggataga agaccttatc cgttagcaaa tgtttcccat
    13861 cttgaagttt tgttagcaga tagttcgtat caggatcaaa ttagtcaatt aaaatttcag
    13921 gcattttcag aggaacatga atcgagagaa gttgtggata gatatgtcgc tgaagctctg
    13981 aaggatccag aaagtcgctt atatattttg ttaaaagacg gtcaggttat tgggacttgc
    14041 acggtagatt tatcgactaa tacgaattac ttctacggtt tagcaatatc ggaacctgaa
    14101 cgtgggaaag gctatggaag ctacttagca aaatccctcg tcaaccaact aattgagcaa
    14161 aatgacaagg aatttcagat tgcagtggaa gatagcaatg taggtgccaa acgtttgtat
    14221 gaaaaaattg gctttgtcaa acagacacag gtggtttatc tgaatgagaa aggagcaagg
    14281 gattccgaag tgtagagaca ttcggactga aatttacttg agctcttagt ttagtgatga
    14341 aactaacagt tcttggattt tagctttcct gattatgatt tatgattaaa atctatgaca
    14401 ccatgtctcg tgatttgcga gaatttgtcc cgattgagga cggcaagatc aagatgtatg
    14461 tttgtgggcc aaccgtgtac aactatattc acgtgggaaa cgcccgttcg acggtagctt
    14521 ttgatacgat tcgtcgctat tttgagtacc gtgggtataa ggttgcctat atttccaatt
    14581 ttacagatgt ggatgataag attatcaacc gtgccaggga agaaggcatc acacctcagg
    14641 aggttgcgga taagtacatc gctgcctttc gtgaggatgt gacggcctta ggcgtgaaac
    14701 ctgcgactcg ccatccgcgt gtagtggagt ttatggcaga catcatccgt tttgtggaag
    14761 acttgatcga gaaaggcttt gcctatgaga gtcaagggga tgtctatttc cgtgtagaaa
    14821 aatcccacaa ctatgctaaa ttggctaata aaaccttgga agatttggag ctaggtgctt
    14881 caggtcgtac cgatgaagaa acggctcgta aggaaaatcc tgtagacttt gctctatgga
    14941 aatcttccaa accaggtgag atttcttggg atagtccttg gggacctgga cgtccgggtt
    15001 ggcatattga gtgttcagtc atgtcgacag agattttagg tgataccatt gatatccacg
    15061 gtggtggagc tgacctagag tttccacacc acaccaatga aattgcccaa tctgaagcta
    15121 aaacaggcaa ggcttttgct aactactgga tgcacaatgg ctttgtcaat atcgacaatg
    15181 tcaaaatgtc taagtccttg ggtaacttca ttacggtaca cgatgccctt aaaactcttg
    15241 atgggcaagt acttcgtttc ttctttgcga cccagcacta ccgtaagcct atcaacttca
    15301 cggaaaaggc agttcgcgat gccgagacca atctcaagta tctgaagaat acttacgaac
    15361 aaccatttac aggaaacgtg gatgcccaag agttacaaaa ctttaaagat aagtttgtag
    15421 cagctatgga tgaagatttt aatgctgcca acggtatcac agttgtcttt gaaatggcca
    15481 aatggatcaa ctcagggaac tatgatgcaa gtgtcaagca agctcttgca gatatgttag
    15541 aaatttttgg aattgtcttt gttgaggaag ttttggatgc agagattgaa gacttgattc
    15601 aaaaacgcca agaggcgcgt gccaatcgtg actttgagac agcagaccaa atccgtgacc
    15661 aattggttac tcaaggaatt aagctccttg ataccaagga tggagtgagg tggacacgtg
    15721 attgatgtca atctcattaa cgggattgcg ctagcctttg agggagatgc ggtgtattct
    15781 atgtatattc gccgccacct catcctcaaa ggtatgacca agcccaataa actccaccaa
    15841 gaggcgacca agtacgtgtc agccaaggct caggctcgtc tgattgctct catgttggag
    15901 gagcagatcc taacggaaaa agaagaagaa atctacaaac gtggccgcaa taccaatagt
    15961 cacacaaagg ctaaaaatgc agatgtcgtg acttatcgta tgtccacggg atttgaagca
    16021 gttatgggct atctccatat gactgagaat ctggaacgtc ttgagagttt ggtttcatgg
    16081 tgcatccaaa aagtggaggg ctagaacatg agggcaaaag aactacaaga ctggtttcct
    16141 gaggctcgga tttcagacca accagtagag aaagagggct atctcacgct tcctttagct
    16201 tctcagcagt ggattttgct ggaggaagct gggctcagcg agcgtgaaaa gcagttggtt
    16261 gcccttttga cccagcagga gcaggctcgt tcgctaaacc cttggtattc ctatctggtt
    16321 gagggcaagg gacaggcacc gcaagttttt aaaaagattc agttggttta ttgccatctt
    16381 tcttattttc agcaggaaaa tctgacttct tggctagata tgatgcggac tctttttccg
    16441 aattgtcaga cagtgctaca ggtcggagct caggattatg ttttcgtgct tcaacaagac
    16501 aaatacactt ctgtaagaga tattttgagt gatacgattg aagcggttga gtatgacttt
    16561 ggacttcgtc tttctatcat gttgggtcag gtttggtctc agacaggaca tcaagcccta
    16621 tcagacttaa tcaaagctga gcgggatttg ttcaagacat ggtggcgtca gggtcaccaa
    16681 ggtgttcata ctttttctca gctctatctt tggagtatgg gagaaagact cgtggacttg
    16741 aagccaatta aagaatgtct acaccagatg attttggatc aagatcagat tcaggaaatc
    16801 attctctctc tttgggaaaa tagtgctgtt ctcactaaaa cagcccagca actctatctg
    16861 caccgcaatt ctctccaata caagattgat aaatgggaag agttgacagg gcttcagttg
    16921 aaagagttga ccgacctgac cttgtgttat caattgattt taggttcttt gtcaactata
    16981 gttggtttgt aaagaagtta ata
//
LOCUS       DAVLVF010000033        16746 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVLVF010000033 DAVLVF010000000
VERSION     DAVLVF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.033"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            39..281
                     /locus_tag="VYN66_001112"
     CDS             39..281
                     /locus_tag="VYN66_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495143.1"
                     /translation="MKINEYLWRTSCLILLIIMSFFAGFGAKLEFTSYNPFLSVKFGL
                     VVALTVFVVQLLAILPAMVKRGNKNENVESNNSCEE"
     gene            244..1635
                     /locus_tag="VYN66_001113"
     CDS             244..1635
                     /locus_tag="VYN66_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEV1495144.1"
                     /translation="MRMLNQTIRVKSKYPYLIRPRNIKIVRLFIQIFVFLLLISSWIA
                     IIPFIARLVFSMFFISLLFFVEKFFRSNFIAKLRNKLNIREALFYMLISLNLYDELDN
                     EVVDTAVLYFDVENNKVVVCAPLFGNRYLKTLKNLEEYLCPTLGLSLLSKKEEIDKIV
                     YVLGQKEEIEQYVFNSNTLTREFFKDIPSSIIKLSNTQKFSLKSNTNLGIYGRTGTGK
                     TIALQWYLFNALAKGCGIADNTYLGIVDGKAADLYRIGELLHEELGEQVAVGSSPQML
                     AQLSRKFIENMDARFKIIKQNSSLNADIYELDELGLVPNFLFVDELASIRDSCGSTKQ
                     GRETWNEILQNLGLIARKGRQAGCHLVLSTQDPNAENIPVELRNQISAVLYLGAPGAD
                     RLKMAFSMCELENVPTVSDRKGEALFYADGLNTVEPVLTIVPFVDLKTKKEFLNVVRN
                     ILPTNDGLPPLAK"
     gene            1891..2310
                     /locus_tag="VYN66_001114"
     CDS             1891..2310
                     /locus_tag="VYN66_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495145.1"
                     /translation="MVYNMTDTNIKIGLLDLEMSQFDIPKKVVILSVRVNNKYKEVNG
                     ENVKTDEVSKITSAVLDADKVKVLTEMGISTDDLKAINLEIIGNLDKIAKLAQNDALL
                     NVPVELIRPKVRLAWNMARNNWSGVKIVCEDIKVLGE"
     gene            2315..3334
                     /locus_tag="VYN66_001115"
     CDS             2315..3334
                     /locus_tag="VYN66_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEV1495146.1"
                     /translation="MNTVRIDYFAVTVKNISPEKVLTDILLIPLENFTLNDWGINKYK
                     QHYSCSEIKVYFNADRLSMGVFIELKGQGCRQYEEFLNSNENNWIALISRLYHHNANF
                     TRLDIAHDIFDGSLDVQRIYDYCKKGLCISKAKHFEYHEKSVLDSGERVGETVAIGSR
                     GNQQWCIYNKRMEQLGKQELVEVSSWTRAELRCWQEKANIIAEQLYLKRPLSSIYFEA
                     INGHYRFIRPNAIDSNRWRRKKVEWWVNYLGTENQTVLSITRAKTTLRESEVWTEKQV
                     AKTLAKVYIAKYQAYDVQKAEDYIQNLLKEGLSKLTDNDEKDIEQYIREQNSSQLWGT
                     DGRSD"
     gene            3338..3520
                     /locus_tag="VYN66_001116"
     CDS             3338..3520
                     /locus_tag="VYN66_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEV1495147.1"
                     /translation="MADTISNKDLISMGYRPSTANAIIHQARELLVSRGYTFYNRKRL
                     MVVPKSVVKELLGMEL"
     gene            3522..4649
                     /locus_tag="VYN66_001117"
     CDS             3522..4649
                     /locus_tag="VYN66_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1495148.1"
                     /translation="MAYIEYKQRGKKRLWSFSIRERSKSLLHKSGFKTKREAKIEAEK
                     VLHKLNTGSVLSSSMTLSELYNEWLDLKILPSNRSVVTKKKYLMRKKVIERLFGNKPV
                     SQIKPSEYQKIMNEYGETVSRNFLGRLNSCIRASIQMAIADKVIIEDFTAYVELFSSK
                     SGQKVEEKYLHAESDYQKVLVYLKNKFDYQKSIVPYVIYFLFKTGMRFSELIALTWDE
                     VDELNEQLKTYRRYNTAIHKFTPPKNNTSIRLVPITSDMLSLLKTLKILQLKTNKELN
                     IDNENNLIFQHFGYVYDVPDIATVNKAIKVMLKELQIFPLITTKGARHTYGSYLWHNN
                     IDLGVIAKILGHKDISMLIDVYGHTLEEKISEEFMAVKSLL"
     gene            complement(4732..4804)
                     /locus_tag="VYN66_001118"
     tRNA            complement(4732..4804)
                     /locus_tag="VYN66_001118"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4769..4771),aa:Lys,seq:ctt)
     gene            complement(5069..6340)
                     /locus_tag="VYN66_001119"
     CDS             complement(5069..6340)
                     /locus_tag="VYN66_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV1495149.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            6610..6810
                     /locus_tag="VYN66_001120"
     CDS             6610..6810
                     /locus_tag="VYN66_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495150.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQ"
     gene            7014..7193
                     /locus_tag="VYN66_001121"
     CDS             7014..7193
                     /locus_tag="VYN66_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495151.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            7330..7509
                     /locus_tag="VYN66_001122"
     CDS             7330..7509
                     /locus_tag="VYN66_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV1495152.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGE
                     LNKYTERGIRKQAGL"
     gene            7546..7998
                     /locus_tag="VYN66_001123"
     CDS             7546..7998
                     /locus_tag="VYN66_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1495153.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            8289..8759
                     /locus_tag="VYN66_001124"
     CDS             8289..8759
                     /locus_tag="VYN66_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV1495154.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVLSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            8778..9866
                     /locus_tag="VYN66_001125"
     CDS             8778..9866
                     /locus_tag="VYN66_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV1495155.1"
                     /translation="MKKIGTYLVYVLAFVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            9847..10275
                     /locus_tag="VYN66_001126"
     CDS             9847..10275
                     /locus_tag="VYN66_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1495156.1"
                     /translation="MELEISDFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKVYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            10413..11363
                     /locus_tag="VYN66_001127"
     CDS             10413..11363
                     /locus_tag="VYN66_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV1495157.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            11365..12108
                     /locus_tag="VYN66_001128"
     CDS             11365..12108
                     /locus_tag="VYN66_001128"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV1495158.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDFIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            12119..13915
                     /gene="pepF"
                     /locus_tag="VYN66_001129"
     CDS             12119..13915
                     /gene="pepF"
                     /locus_tag="VYN66_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1495159.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(13975..14652)
                     /locus_tag="VYN66_001130"
     CDS             complement(13975..14652)
                     /locus_tag="VYN66_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1495160.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQKKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEQGKEF
                     LEKEWGI"
     gene            14899..15669
                     /locus_tag="VYN66_001131"
     CDS             14899..15669
                     /locus_tag="VYN66_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495161.1"
                     /translation="MLYLLGKERVKKLSVKNLKKQYGSYEAVKGISFELGEGECLGIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDFLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIKEEGTVEDLLIKYDADNLETLFLGLTGTEKEDLHV"
     gene            15662..16426
                     /locus_tag="VYN66_001132"
     CDS             15662..16426
                     /locus_tag="VYN66_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1495162.1"
                     /translation="MFKLSFHSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TNDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
BASE COUNT         5040 a         2834 c         3806 g         5066 t
ORIGIN      
        1 tctctttatt tttaaaattt gaaaggaaaa atacaaaaat gaaaattaat gaatatttat
       61 ggagaacgag ctgtcttata ctgctaatta tcatgagctt ctttgcaggc ttcggagcta
      121 aattagagtt tacatcttat aacccgtttt tatctgttaa atttggtttg gtcgtagctc
      181 tgacagtctt tgtagttcaa ttgttagcca tactgccagc catggttaaa agaggaaata
      241 aaaatgagaa tgttgaatca aacaattcgt gtgaagagta aataccctta cttgattcgt
      301 ccccgaaata ttaaaattgt aagattgttt atccaaattt ttgtcttttt actcttaata
      361 tcttcgtgga ttgctataat cccatttatt gctcgtctag tcttttccat gttttttata
      421 agtcttcttt ttttcgtaga gaagttcttt cgttctaatt ttatagcaaa attgagaaac
      481 aagctcaata tcagagaagc cttgttttac atgttaatta gtttaaattt atatgatgag
      541 ctcgataatg aagtagtaga tacagcagtt ttatattttg acgttgagaa taataaagtt
      601 gttgtctgtg ccccactatt tgggaaccgt tatctgaaaa ccttgaaaaa tttagaggaa
      661 tatctctgtc ccactttggg cttgtccttg ctgtctaaaa aagaagaaat cgataaaata
      721 gtatacgtgt tagggcagaa agaagaaata gaacaatatg tttttaatag taacacatta
      781 acaagagaat tttttaaaga tattcctagt tcaataatca agctgtcaaa tacacagaaa
      841 tttagtctaa agtccaacac taacctaggt atatatggaa gaacggggac aggcaaaaca
      901 atagctctgc aatggtactt atttaacgca ctggctaaag gttgtggtat tgctgataat
      961 acttatttag gcatagtaga tggtaaggca gctgacttgt atcgtattgg tgaacttctt
     1021 cacgaagaac tgggagaaca agtggcagta ggctcttcac cacaaatgct tgcccaatta
     1081 tcgagaaaat tcattgagaa tatggatgca agatttaaaa ttatcaaaca gaatagttca
     1141 ttgaatgctg acatttatga gctagatgag ctgggactgg tacctaattt tttgttcgtt
     1201 gatgaactcg cttctattcg tgatagttgt ggctctacga aacaaggtag agaaacatgg
     1261 aatgaaattc tacaaaattt aggcttgatt gcacgcaaag gacggcaggc aggttgccac
     1321 ctagtcctat ctactcaaga ccctaatgcc gaaaacatcc ccgttgaatt gaggaatcaa
     1381 atttcagcag ttttatacct tggtgcccca ggggctgata gattgaagat ggcttttagc
     1441 atgtgcgaat tagaaaacgt tcctacagtt tcagatagaa aaggggaggc tctcttttat
     1501 gctgatgggc ttaatacagt agagccagtg ctaacaattg tgccatttgt tgatttaaaa
     1561 actaagaaag agtttttaaa tgtagttaga aacatactgc caactaatga cggtcttcct
     1621 cccctagcga aatagggcga ttgccggagg cagaatcgag cggagcgctc tcttttcgct
     1681 aggggaggga gagccaaccg actgctttgc aggaggtcag aaaaaacatc cccagtacta
     1741 atagggggtg cacacataca tgaaatgtac tctaaaccct tgatacgtga ggggttacgg
     1801 cttactagtt gcaccaaggg gtcgcaaact cttgggagag tagggatgag aggtgtttcc
     1861 atgtatgata cggaattaaa ggagaaaaat atggtttaca atatgacaga tacaaacatt
     1921 aaaattggat tgcttgacct tgagatgtca cagtttgata ttcctaagaa agtagtgatt
     1981 ttatcagtta gggtgaacaa taaatacaaa gaagtcaacg gcgaaaacgt gaagacagat
     2041 gaagtaagta aaattacttc tgctgttctt gacgctgaca aagtgaaagt tctaactgaa
     2101 atgggaattt ctactgatga cttaaaagct attaacctag aaattatcgg aaatctagat
     2161 aagattgcaa aacttgctca aaatgatgct ttgttgaatg ttcctgttga attgattcgg
     2221 cctaaagttc gactagcttg gaacatggct aggaataatt ggtctggtgt aaaaattgtg
     2281 tgtgaggata ttaaagttct aggagaataa tcctatgaat acagttagaa tagattattt
     2341 tgctgtgaca gttaagaata tttcgccaga aaaagttctg acagacatcc ttttgattcc
     2401 tcttgaaaac tttaccttga atgattgggg cataaataag tataagcaac attattcttg
     2461 ttcagaaatt aaggtgtatt ttaatgctga taggctttct atgggtgtat ttattgaatt
     2521 aaaagggcaa ggttgtcgtc aatatgaaga atttttaaac agtaacgaaa ataattggat
     2581 tgctttgata agtcgtttat atcatcataa tgccaacttt actagactag atattgccca
     2641 cgacatattt gacggtagct tagatgttca acgaatatat gactattgca agaaaggatt
     2701 atgtatctcg aaagcaaaac actttgaata tcatgagaaa tcagttctag attctggtga
     2761 gcgtgtagga gaaacagttg caataggttc acgaggtaat cagcagtggt gcatatataa
     2821 taaacgcatg gagcaacttg gtaagcaaga gcttgtagaa gtttcatctt ggactcgcgc
     2881 agagcttaga tgttggcagg agaaggctaa tataattgca gagcagttat atttaaaacg
     2941 tcccttatcg tcaatatatt ttgaggctat caacggtcat tatcgcttta ttcgtccaaa
     3001 tgctatagat agtaatcgtt ggagaagaaa aaaggttgag tggtgggtaa actatctagg
     3061 gacagaaaat cagacagttc tgagtattac aagggctaag acgacgttac gggagtctga
     3121 agtttggact gagaaacagg ttgcaaaaac attagcaaaa gtctatattg caaagtatca
     3181 ggcctatgat gttcaaaagg cggaagatta tattcaaaac ttgctcaaag agggactttc
     3241 aaagctaact gataatgatg aaaaggatat agaacaatat ataagagaac agaatagttc
     3301 ccaactttgg ggaaccgatg gaaggagtga ctgacatatg gcagatacta tatcaaataa
     3361 ggatttgatt tccatgggct ataggccatc aacagctaat gctattatcc atcaggcgag
     3421 agaattactt gtatcacgag gctatacatt ttataatcgc aaacgtttga tggttgttcc
     3481 aaaaagtgtt gtgaaagagt tgttgggaat ggaattgtga aatggcttat atcgagtata
     3541 aacagcgtgg taagaaaaga ctttggtcct tctctatacg agaaaggagc aagagcctac
     3601 tccataaaag cggatttaaa acaaaacgag aagctaaaat agaagcggag aaagttcttc
     3661 ataagttaaa tactgggagt gtcttaagct ctagtatgac tttatcggag ctttataatg
     3721 aatggcttga tttaaaaatt ttacctagca atagaagtgt agttactaaa aaaaaatatc
     3781 tcatgagaaa gaaggtcatc gaaaggttat ttggaaataa gcctgtatca caaattaagc
     3841 ctagcgagta tcaaaaaatt atgaatgagt atggagagac tgtatcgaga aatttcttag
     3901 gaagattgaa ttcttgtatt cgggcaagta tacagatggc tattgctgat aaggtgataa
     3961 tagaggattt tactgcttat gttgagttgt tctcttctaa gagtggacaa aaggttgagg
     4021 aaaagtattt gcatgctgag tcagattatc aaaaagtttt agtatatttg aaaaataagt
     4081 ttgattatca aaaatctatt gtaccgtatg taatttattt cctttttaaa actggcatgc
     4141 gtttctccga attgattgct ctaacttggg atgaagttga cgagcttaat gagcaactta
     4201 aaacttatcg tcgttacaac accgctattc ataagtttac tccacctaaa aacaatactt
     4261 caataagatt agttcctatt acttcagata tgctttcctt gttaaaaaca ttgaaaatat
     4321 tgcaattaaa aacaaataaa gaattaaata ttgataatga aaataatttg atttttcagc
     4381 attttggcta tgtctacgat gttccagata ttgcaacagt caataaagct ataaaagtaa
     4441 tgttgaagga attgcaaatt tttccattga ttactaccaa aggagctaga cacacttatg
     4501 gtagttattt atggcataat aatattgatt tgggagttat agcgaaaatt ttaggacata
     4561 aagatatctc catgttgatt gacgtttatg ggcatacctt ggaggaaaaa attagtgaag
     4621 agtttatggc tgtgaaaagt ttgttgtaaa aacacggcaa attgcacgac aatttttaaa
     4681 aggaaggcaa aaaaagccct atattataag gcttttcctg ttgtatttat atgcccccta
     4741 cagggatcga acctgtgacc cacggattaa gagtccgctg ctctgccagc tgagctaagg
     4801 aggcaaagaa aaaagctgta ttggtgccga aacttcacgg tttgtattga acccgcgcaa
     4861 ttaagcaggt gggcaactcg ctctaactga agctgtttcc gtgtgagacg gcctacatgc
     4921 tgttagaaga cttttgtttc cctaataata caaaaaatag tcggtcaaca cttaagtgtg
     4981 aagtcgtaca ccacagcgtt tctatactta tatgatacca ctttttcaaa aaaaatcaag
     5041 aggaaagtgc aattttttga aaaggatttc agatttttcg caaacggtcg atagcttcct
     5101 ttctttgagc caaagcccgt tcatatttac cagtatcttc tgcttggaaa tagtgatgat
     5161 tacgaatttt ttctggcaga tagtcttgct tgacccaatt tccaggatag ttgtgtggat
     5221 agagatagtc ttgggcattc cccagttcct tgcttccact gtagtgccca tcacgtaggt
     5281 gtcgcggaat aggcaagtgc cctgatgttt tgaggtcagc aagtgcctta tccatagcta
     5341 cataggctga gttggatttt ggagaaaggg ccaaatcaat cacgacattg gcaatgagaa
     5401 tgcgggcttc tgggaaccca atcttctggg cagcatccag agcagtcacg gtatgaatct
     5461 gggcctcagg attggctaag ccgatatctt cataggcgat aacagtcaag cgacgagcga
     5521 gactaggcag atccccagcc tcaatcaagc gggcagtata gtggagactg gcatccacat
     5581 ctgagccacg aatagacttt tgcagggcag atagaacatc atagtgaccg tctccatcct
     5641 tgtccatagt gatgtagctt ctctgaagac tattttccat gatgtctaag gtgatgtggc
     5701 gaatgccctc gtcattctca ggggtagaga gaacagctaa gtccagtgag ttaaaggcag
     5761 agcgaaggtc tccgtttgta gaggttgcga tgaaatccag cgcatcctca tctagttcta
     5821 ttggaaaatc aaaaccacgt tcagggttac ttagagctat ctgcagggcc tctttgacgt
     5881 cttggttaga cagaggttcc aactcgaaaa tttgaactcg gctacgaatg gcaggagtga
     5941 cagagaagaa aggattttca gtcgttgctc caatcatgat gaccagtcca ctttccaaga
     6001 gaggcaagag gaagtcttgc ttggtcttat ctagtcgatg aatttcgtct agcaatagga
     6061 cgagaccacc agaaaattta gcttcttccg agatttcttg cagtcgcttt ttactatcaa
     6121 ctgtcgcatt gaaagttcga aaggcatact tggtcgttcc agcgatggca gaggcaatac
     6181 tggttttgcc gattccagga gggccatata gaatcatgga ggacaggcgg ttggcttcca
     6241 ccatgcggcg gatgattttt ccaggtccga ccagatgctc ctgaccgatg acctggtcga
     6301 tggttttagg gcgcatgcga agcgcgagat tgtctggcat agcagtcctt tctagcgtgg
     6361 atttgagtag aatgactcat ctactatatc ttactatgtc cattctagcc tattagttgt
     6421 gatatttcaa tggttttgta ataatcaggg agtgagatag aatctttttt actatttaat
     6481 ttaatgagga gaggtatcag catgagagat ttgattttcc ttaaagctca aaataggata
     6541 gttgagccta aagaagtgga agtgctgact tgagaaataa tattgctgaa tgtcattgta
     6601 tgaactataa tgaaaagaga ggtgataagc aatggaaatg atggaccaag tcaggaaatt
     6661 ttgattttta caaaacaaat tcgtcattgg attcttagtg atcaagttat ttcagggaaa
     6721 agaaaacttt tcttccggga agatactcca aaagagattt tagatttgta cgaaaacatt
     6781 aaatctaaac ttgattttgc ttaccaataa gtttattcta ataatgggtt gaaaaaatat
     6841 gaaaaataga ataattgatg tttttgaagt agtaaatcgc atccttgtaa taactgttga
     6901 aaatcctgat ttcgaagact tgagagttaa tcatactcaa tgaaaatcaa agagcaaact
     6961 aggaaactag ccgcaggctg tacttgagta cggtaaggcg acgttgacgt ggtttgaatt
     7021 tgattttcga agagtatcag tttgtaaaaa ttggagataa aaaatataga gtacgcagtg
     7081 ttcctatgat tcattcaact ccgcctcaat ctgtcttaga tcgagatact tttacaattg
     7141 actatacaga tgatgaattg ctcgataaag aagcagtttt tactactcat tagaaaaata
     7201 ggtctgaata aattggcttc ccttagaacc acatctaggg gcttttatgt ataatagaat
     7261 aataagtatc actcctttac aatacgtgta acacgtgtta taatggtgtt gtcaggaggt
     7321 aaaagcgcta tgcctatgac acaaaaagag atggttaaac tccttacggc ccatggttgg
     7381 ataaagacta gaggcggtaa aggctcccat attaaaatgg agaagcaagg ggaaagactt
     7441 atcacaatcc ctcatggtga gctgaataag tacactgaaa gagggataag aaagcaagct
     7501 gggttgtaaa attcagcccc tgttttttga tatggaggta gtgaaatgtt agttacgtat
     7561 ccagctttat tttattatga cgacacagac ggaacagaag cgacttattt tgtccatttc
     7621 ccagattttg aatactcagc tacacaagga gaggggattt ctgaggcttt ggctatgggg
     7681 tcggagtggc tagggataac tgttgcagat ttgatagaga gtgatggaga gttacctcag
     7741 ccatcagata tcaatagctt gtctttaatt gataatgatc cttttaaaga tgatgaagat
     7801 ttcgtgtcaa cctatgacct tgataaatct tttatttcta tggtatctgt tgatgtatca
     7861 gaatacctag gaagtcagga acccataaaa aagactttga ccataccaaa atgggcagat
     7921 aagttgggac gagaaatggg acttaacttt tctcagactt tgacagacgc tattgcagat
     7981 aagaaagttc aagcctaaga ttaagcgtgg tatcgtggta atagtcaata aaaaagcacg
     8041 tccacttgtg ctagttactc gcctattgat tttatagatt tattagccct ttcgcgggct
     8101 ttttatattg atttttatga gaaatgaaga aatcagactt ttaagaaaat cactgatacc
     8161 aagggtttaa atgaggtaat atggtataat taggacataa aataattttg tggtaagatg
     8221 gtagtatcta ttttagcata tttccgagca atcggggcga ttaaagagtc gcatagaaag
     8281 aggacaaaat ggcaacatac ggatttttag atgttttaga ggaagagttg gacaagaact
     8341 ttccctttga ctttgagatt agctgggaca aacgcaacca tgcggtagaa gtgagttttc
     8401 tattggaagc gcaaaatgct gcaggtgtgg agatggtgga tgaagacgga gaggttttat
     8461 cagatgacat tctttttgag gaagcggtcc ttttctacaa tcctgccaag tcaacagtca
     8521 atgaggaaga ctatttgact gttattcctt acctgcctaa aaaaggattt tcccgtgaat
     8581 ttttagctta ttttgcgctt ttccttaaag atactgctga ggttgggcta gatgtcctca
     8641 tggacttttt ggaagaccca gaagcagagg aatttgtcat ggaatggaac caagaagtct
     8701 ttgaagaagg aaaagttggc ttggaagagg gagaatttta tccttatcca agatactagg
     8761 agttggttgg aggtgttatg aagaaaattg ggacttattt ggtttatgtg ctagcttttg
     8821 tctttattat gctagctttt gcttgtggaa caatcgcatt tgcagagttg gggtattccg
     8881 cagttttagc ctttactttt ggttatgcct tcgctcttct aagcatgtat ttaatcttta
     8941 ttcttcatga gctgggtcat gctttttgtg gctacttgac aggctatcgg ctggtggctt
     9001 ttggattagg acattttatt ttgaccaaaa agtcaggcag gtttcatctt agtagaacag
     9061 ccattctgaa aaatgttggt gctcaataca ttggtttaaa agaggatgaa agcgatcaaa
     9121 gaatcatcct gatgctttca ggaggcttga tggttcatct cggcttgata ttattggcga
     9181 tagtgtttgg atttttgaca agaagctggt attttgcagg gacttggatt tttcttaatt
     9241 tatctttctt cctaaataac attttgccag tcgacatcac cgatggagca aaaatttggg
     9301 aattactaca acaccctgaa aataccaaat acgcctacct ggtattgaga cattctgctc
     9361 aaactttgct ggctcctcaa gaatatgatt tgaaagactt tgttcaagca gttcctgagg
     9421 atgcacgagg gagttttgcg gatggtgtat taggtatgca aggtgaagtt tccattttgg
     9481 aagggaagga agaagtagct aaacagcagt ttcaagcatt acttgagagg acaactactc
     9541 cgatgatgca gacagttgcc caactatccc tgcttcatat cgccttgttg gaaggagatt
     9601 ttgagcaagc ggagcaatat gctagtattc gacaggtcaa atcgtttctg tctctcaaac
     9661 tgtccaatct tcaagtgata caagcttggt atcaatttaa ggtaaagaaa gatgtagatc
     9721 gaactcgtaa ggctctcaag attgctagac agaaaatgga tagtagtcga atgttgaggg
     9781 acgagaaacg ctattatgaa aacttgttag cagagctgga aaaggaaatg ttagaaggaa
     9841 ctgaagatgg aactagaaat ttctgatttc ccaggctgta agattgcttt gttttgtggg
     9901 gataagctct tgactatctt acgtgatgat aaggcaagta ttccctgggc caatatgtgg
     9961 gaactgccag gtggtggacg tgaaggtgat gaaagtcctt ttgagtgtgt agcgcgtgaa
    10021 gtttatgaag aactgggcat tcatctgact gaagattgtc tgctttggag taaggtttac
    10081 ccaagtatgc tttttgcaga caaacagtcc gtttttctag ttggtcagtt gacacagaac
    10141 cagtttgaca gtatcgtatt tggagatgaa ggacagggct atcagctgat gaatgttgag
    10201 gaatttctta gttccagtca agttgtccct cagttgcaag agagattaaa agattattta
    10261 aaagtaagtg attagaaagg atggttcagt taaatttcta aactgaaccc gccctaaaca
    10321 ctgtgccaaa aagataaact tctcttagac acaagcgtct tcagagaatt tcctattttg
    10381 gttttgtgtt ttacgggctt ggtatcttaa taatggaaac atggcaagag ttaaaagtta
    10441 cagtgaagcg tgagggagag gagttggttt ccaatctctt gattgagctg ggagcgcaag
    10501 gagtcgcaat cgaagacagt atggactatg tgggaaatgt tgaccgcttt ggcgagattt
    10561 tcccagaggt ggagcagcaa gaagaaatcg ttgtgacagc ctactaccct gacacggtgg
    10621 atgtgacagt ggttgaggcg gacttgcagg ctcgcatatc agaattgacg gattttatgg
    10681 atttgggaga gttaaaaata ggtacgactg ccttggctga ggaagactgg gcagacaact
    10741 ggaagaaata ctatgagcca gctcgtatca ctcatgattt gaccatcgtt ccctcttgga
    10801 cagactatga ggcgactgcg ggagaaaaaa ttatcaagct ggatcctggc atggcttttg
    10861 gtactggaac ccacccaact accaagatga gcctttttgc cttggaacag gttcttcgtg
    10921 gtggcgaaac ggtgctagat gtggggactg gttcaggggt tctctctatt gccagctcgc
    10981 ttctgggggc taaagaaatt ttcgcctatg acctggatga tgtggcggtt cgtgtggctc
    11041 aggaaaatat tgagctcaac cctggcatgg aaaatatcca tgtagcggct ggagatttgc
    11101 ttaagggtgt ggaaattgag gcagatgtga ttgtggctaa tatcttggcg gatatcctca
    11161 ttcatctaat agacgatgcc tatcgtttgg ttaaggacga aggctacctg atcatgagtg
    11221 gcattatcaa ggacaagtgg gacatggtgc gccagtcggc tgagtcagct ggatttttcc
    11281 tcgaaactca catggttcaa ggggaatgga atacctgtgt ctttaagaaa accaaggata
    11341 tctctggtgt gattggaggc tagcatgcag cagtattttg taaaaggcag tgctatctct
    11401 cctgtcacta tcgaggacaa ggaaaccagt aagcatatgt ttcaggttat gcgcttgaaa
    11461 gaagaggatg aggttacctt agtctttgat gatggcatca agcgcttggc gcgcgtgctg
    11521 gatatggaaa atcgtcagtt tgagttggtc caagaattaa atgacaatgt ggaactacca
    11581 gtccaagtga ccatcgcatc tggatttccc aagggagaca agctggagtt catcgctcaa
    11641 aaagtaaccg aactgggtgc tagccaaatc tgggcctttc ctgcagactg gtcagttgcc
    11701 aagtgggatg gcaagaaatt gggtaaaaaa gttgaaaaac tagaaaaaat tgcccttgga
    11761 gcagccgagc aaagcaagcg taatattgta ccaagtatta aacttttcga gaaaaaagca
    11821 gattttctag ctcagctgga ccagtttgac tttatcatag tagcctatga agaatcagct
    11881 aaagaaggag aagccgctgc gcttctgcaa gcagtcactg gtcttgaaac aggagccaaa
    11941 ctgctcttta tctttggtcc agaaggcggt ctctcacctg cagaaatcga aagttttgaa
    12001 gctaagggag ccgttttggt aggcctaggt cctcgtattt tgcgagcaga aacagcaccg
    12061 ctttacgcct tatcagccct tagtgtttta gtagaattag agaaataaga ggaagaaaat
    12121 ggaacaaaaa caccgttcag aatttccaga gaaggaactc tgggacttaa cagccctata
    12181 ccaagaccgt gaggatttct tgcgtgcaat cgagaaagct cgtgaagaca tcaaccagtt
    12241 tagccgtgat tacaagggca atcttcacac ttttgaggat ttcgagaagg cctttgcgga
    12301 attagaacag atctacattc agatgagcca tattggcaac tatgctttta tgcctcagac
    12361 gacggactat agcaatgacg aatttgccaa tattgcccaa gctgggatgg aatttgaaac
    12421 agatgccagc gtagccttga ccttctttga cgatgccttg gtggtagcag acgaggaagt
    12481 cttggaccgt ttgggtgaat tgcctcactt gacggcagct attcgtcagg ccaaaatcaa
    12541 aaaagcccac tatctagggg ctgatgtgga gaaggccttg accaatctcg gtgaagtttt
    12601 ctatagtccg caggacattt atactaagat gcgagctggg gattttgaaa tggctgactt
    12661 tgaagcccat ggcaagacct ataaaaacag ctttgtcacc tatgagaatt tctaccaaaa
    12721 tcacgaggat gctgaggttc gtgagaaatc cttccgttcc ttctcagagg gacttcgtaa
    12781 gcaccaaaat acggctgcag cagcctatct ggctcaggtc aagtctgaaa aactcttggc
    12841 tgatatgaag ggatacgact ctgtatttga ttatcttcta gctgaacaag aagtggaccg
    12901 tgtcatgttt gaccgccaga ttgacctcat catgaaggac tttgcaccag tcgctcagag
    12961 atacctcaag catgttgcca aggtaaatgg tcttgaaaag atgacctttg cagactggaa
    13021 attggacttg gacagcgccc tgaatcctga agtgactatt gacgatgcct atgatttggt
    13081 catgaagtcg gtagaacctt tggggcaaga atattgtcag gaagttgctc gttaccaaga
    13141 agagcgctgg gtggactttg ctgctaacag tggcaaggat tccggtggtt atgcggcgga
    13201 cccatatcgc gtacaccctt atgtactcat gagctggaca ggccgtttga gcgatgtcta
    13261 taccttgatt catgaaatcg ggcattctgg tcaattcatc ttttcagaca atcaccaaag
    13321 ctatttcaac gcccacatgt cgacctatta tgttgaagca ccgtcaacct tcaatgaatt
    13381 gctactcagt gattacttag agaaccagtc taatgaccca cgtcaaaaac gcttcgctct
    13441 ggctcatcgc ttgacagaca cctacttcca taactttatc acccacctct tggaagccgc
    13501 cttccagcgt aaggtgtata cattgattga agaaggggag acctttggag caagcaaact
    13561 caacagcatt atgaaggaag ttttgacaga tttttggggc gatgccattg aaatcgacga
    13621 cgatgcagct ttgacttgga tgcgccaagc tcactactat atgggcttgt atagttacac
    13681 ttactctgct ggtcttgtca tctcgacagc tggttacctt catcttaaaa actcagaaac
    13741 tggagctgaa gactggctca atctcctcaa atcaggtggt agcaagacac cacttgagtc
    13801 agccatgatt atcggtgctg atatttcaac agacaaacca ctccgtgata ccatccaatt
    13861 cttgtctgac acagttgacc agattatcgc ctacagtgct gagttggggg agtaagggga
    13921 tagagaggtt gttatagaat gggtatggac agttatttgc ttaaaagtcc atgtttaaat
    13981 cccccattcc ttttcgagga attctttgcc ctgctctgta atggtaagtg aacgtggcgt
    14041 ttgtgcaggg atgaccaatt gtttttcact gaaaaattct aaaagagcat tgccgagagc
    14101 tccgccgaca tgatttttac gttcgctcca atccaaacat ttagggcaaa atttccgttt
    14161 tttctttttc aattcatccg tatcaatgtg taattttttt tggaaataag acttcccagc
    14221 ttcagtgagt tggaagtctt tttctgattc gctgaggaga ttttcttgca agagataatt
    14281 ggtaatggaa atgccaagct caccagcaat atggtcatag caggtgcgag cgacctttaa
    14341 ttcctttttg tgaatgtgtt gtttataact tttgacatca cgctgaggag agtagaagct
    14401 gagaaattcc agcatttctg caatggcagc actttttaaa gaataataat gaaaacgtcc
    14461 ataggtttgt agagtggtta cctgcgcttc ggtaagtaat ttgaggtgat agctgacagt
    14521 atgttgcttg attttggtaa agctggcaat ttcgttaacc gtgtgagctt ttccatccat
    14581 tagaatatct aaaatgtgca tccgagaagg gtctgataag agttttgtga tgatgaaaat
    14641 atctggttcc ataaaaattt tctccaaatt tcatatatcg attattttct atcttttatt
    14701 atactataat caaagcacaa gaaaaataga ttttaaaaat gttttactag agaatatttt
    14761 aaattttatt gtctgagttt aaatggaggt aaaattacaa aagtaattgc agcagtatcc
    14821 acagttgaag gacgttaatc ttaagattac attctcaaca ttttttcaaa agtgagatga
    14881 attaacaaag tatccaacat gctctatttg ttggggaaag aaagagtgaa gaagttaagt
    14941 gttaaaaatc taaaaaagca atatggttct tatgaagctg tcaagggaat tagctttgaa
    15001 ttgggagaag gagagtgttt gggtatcttg ggaatcaacg gtgctggcaa aacatccact
    15061 ttaaagatga tttactcact gacctcaatt tcctcaggag agattgatat cttggggcaa
    15121 aatattgaaa ccaatcgtcg taagagtaaa tcttgtctgg gtattgtagg gcaggaggat
    15181 atgctggata tgaccttgaa tgtttatgat aatctttttg ctcacggtct atgttatggt
    15241 atctcatctg tcgaacttag gaagagaatc gattttctct tggattttgt tcaactgagc
    15301 aaacatgcgc aaaagatggt gaatcagctg tcgggtggga tgcgacgacg tcttgtcttg
    15361 gcaagagctc tcatcaatcg gcctgatatt atcattttag atgaaccgac agttggttta
    15421 gacattcaat cccgcaatat tatttgggat aaacttttgg agttgaagga attaggtgtt
    15481 tccatcatta ttacttccca ctatatgaat gagattgaat atttgacaga tagggtgctg
    15541 atgctccacc aagggactat caaagaagaa ggaactgtag aagacttgct catcaagtat
    15601 gatgctgaca accttgagac gctttttttg gggttgactg ggacagagaa ggaggacttg
    15661 catgtttaag ttatcatttc actccatcgg acacgttgtg gtacgcaact acatgagttt
    15721 tagaaattta tttaaaattt caattgtccc caaccttatc gaccccctct tttatctttt
    15781 agcaatggga tttggagttg gtgcttattt gacgcatgtt aacggtatgc tctaccgtga
    15841 ttttgtcatt acaggtttaa ttgctgcgac tgccatgtct gcggccactg cagaaacaac
    15901 ggtcaatgcc tttatccagt ataagattga aaagacttat gatgctatgt tgatgacgcc
    15961 aatcaatacg aatgatattg tggtgggtca ggctatctgg gcaggtattc gtgcagtgat
    16021 tttcggtggg attttttggc tgatttccat gcttatcaca gcgcattttc atgtcttgat
    16081 gttattgatt cctctgattt tatttatagt gggttatttg tttggtgttt tgggactaat
    16141 ctttacctac ttggcgccgt ctagggaatt tctgaattac tacaatgttt tggtaatccg
    16201 tcctctttat atgttttcag ataccttctt tcccattact tccttgcccc caatacttgg
    16261 tgatttgact tggttttctc ccttgtatca cgctacccgc atgattcgta ttatatgggg
    16321 aggtgaggtg acaggtttgt atcttcatct tatctggttg atagccttgg cgctcttgat
    16381 aacggtgctt cctatgtata tgttacatcg tagatattat agatagtcta attatttgtg
    16441 atgttgtagt catattctaa tttatactaa acgttattcg aatttggaca ggtatgggaa
    16501 ctgtgcctat caatattgta atttatggaa gttgaaatag tacggttcag tttttaaaat
    16561 attgttagaa attgttttaa attccctaat caatttgtat agtttttatt tcactttatt
    16621 ataactagtt tttcgcaatt tttgagacag gaatgtgatt tttgtgataa ggctctatca
    16681 ttacaagttc atttgtggtc atatggaata aatcatttat atatcttttc agaaatatgc
    16741 tgtttc
//
LOCUS       DAVLVF010000034        15417 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVLVF010000034 DAVLVF010000000
VERSION     DAVLVF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15417
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.034"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..801
                     /locus_tag="VYN66_001133"
     CDS             <1..801
                     /locus_tag="VYN66_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1495163.1"
                     /translation="LSVNYDSGVSGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLET
                     LRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVST
                     QYANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDK
                     ELNIHAEEAKIRLRNLNAAIENVQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPL
                     LEDYLRGSYDEVETLETLKKAFELTNNEQKDQAVADDNEGDENDDADY"
     gene            788..2104
                     /locus_tag="VYN66_001134"
     CDS             788..2104
                     /locus_tag="VYN66_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV1495164.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLYINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFFHNDSNVKGV
                     IDVRSHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKITRRNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            2279..3265
                     /locus_tag="VYN66_001135"
     CDS             2279..3265
                     /locus_tag="VYN66_001135"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV1495165.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCVKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            3329..3400
                     /locus_tag="VYN66_001136"
     tRNA            3329..3400
                     /locus_tag="VYN66_001136"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3362..3364,aa:Arg,seq:ccg)
     gene            3583..4281
                     /locus_tag="VYN66_001137"
     CDS             3583..4281
                     /locus_tag="VYN66_001137"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV1495166.1"
                     /translation="MKELQTVLKNHFAIEFTDKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            4272..7811
                     /gene="smc"
                     /locus_tag="VYN66_001138"
     CDS             4272..7811
                     /gene="smc"
                     /locus_tag="VYN66_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV1495167.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLATLEDKRSS
                     LSQEKSDKESSLALLEENLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSHQLSQKQADQLEKLKEQLATAKEKAGQQKAELETAKEQVQK
                     LLADYQVCAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDENAATKAIDLLKR
                     NRSGRATFLPLTTIKARTISSQNQDTIAASPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELNRLSEGEWQADKEKCQERLTTITSEKNNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTDLLSQQADEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLDAIEQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            7808..8602
                     /locus_tag="VYN66_001139"
     CDS             7808..8602
                     /locus_tag="VYN66_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1495168.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            8602..9420
                     /locus_tag="VYN66_001140"
     CDS             8602..9420
                     /locus_tag="VYN66_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1495169.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            9424..10701
                     /gene="ftsY"
                     /locus_tag="VYN66_001141"
     CDS             9424..10701
                     /gene="ftsY"
                     /locus_tag="VYN66_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV1495170.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVIEVSQKEVKEFPHSEEGNTEFLETVE
                     ENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(10747..12234)
                     /locus_tag="VYN66_001142"
     CDS             complement(10747..12234)
                     /locus_tag="VYN66_001142"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV1495171.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWD
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRLE"
     gene            complement(12383..13123)
                     /locus_tag="VYN66_001143"
     CDS             complement(12383..13123)
                     /locus_tag="VYN66_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1495172.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(13123..15288)
                     /locus_tag="VYN66_001144"
     CDS             complement(13123..15288)
                     /locus_tag="VYN66_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1495173.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
BASE COUNT         4762 a         2783 c         3432 g         4440 t
ORIGIN      
        1 ttgtcagtaa attatgatag tggtgtttca ggatggtcac tacctagcaa atatgtttac
       61 gagttgtata aagataaaaa ttataataag caagaatact acaaaagtgg tggaaaaact
      121 gtcctagaaa cattgagaaa gagatttggt ttgaaagact atgtttcccc aacagaaatt
      181 gatactgata agaaattcgt cttcatcatc gatgaaatca atcgtgggga gatttctaag
      241 atttttggcg aactcttttt ctctatcgac cctggctatc gtggtgaaaa aggaagtgtt
      301 tctacccaat atgcaaatct acacgaaact gatgaaaagt tctatatccc cgaaaatgtt
      361 tacatcatcg gaactatgaa tgatattgat cgttcagtgg atacctttga ttttgctatg
      421 cgtcgtcgtt ttcgttttgt tgaagttact gccgagggtc aagttggcat gttggataaa
      481 gagctgaata tccatgcaga agaagcaaaa attcgtctaa gaaacttgaa cgctgctatc
      541 gaaaatgttc aggaattaaa cagtcattat catattggac caagttattt tcttaagttg
      601 aaggatgtag attttgacta tgaattactc tggtctgatt atattaagcc tctcctagaa
      661 gactacttgc gaggttctta tgatgaggtt gaaactttgg aaactttgaa aaaagcattt
      721 gaactgacaa ataatgagca aaaagatcag gcagtagctg atgacaatga aggtgatgaa
      781 aacgatgatg cggattactg ataatcaaca caagattatt aaagaaaaat ttgttgaaga
      841 atatcctaaa ctaagcaatc ttcttttaga cagaaccttg gaaagtctat cccaagatga
      901 acgtattttc atttttccaa atgatttgac tcatactcct gatttggata aggaccaaaa
      961 gatttttgaa acagtcaatc agaaaatcaa gacagggaac gtgattggtt ttcttggata
     1021 tggtcaggaa agattaacga tttcctcacg attttctgat gagagtaatg accatttttt
     1081 gcattatctc ttaaacaagg ttctttatat caatctcact agtttagatg ttgctttgtc
     1141 tcgtgaagag aggctttatc aacttttgat gtatctcttc cccaagtatc tacaagctgc
     1201 tattcgaaaa ggtctttata aggaatatca tcgatttttt cataacgaca gtaatgttaa
     1261 gggagtgatt gatgtaagaa gccatctcaa gaaaaatctt cctttcacgg gaaatattgc
     1321 ctacgcaacg agagagttca cctatgataa tcccctcatg cagttggtcc gtcacactat
     1381 tgaatacatt aagaatcaga aaagcattgg tcaaggggta ctagataatc tctcaactag
     1441 tcgtgaaaac gtatctgaaa tcgtgcgtgt aacgccctct tataaactag ctgatcgtgc
     1501 taagattact cggagaaatc aatctaaacc tatacgtcat gcatactttc acgagtacag
     1561 aaacttacaa gaactttgtc tgatgatcct aaaccaagaa aagcacggtt tagggtatca
     1621 agatcaaaaa atctatggta ttctctttga tgttgcctgg ctttgggaag agtatgttta
     1681 caccttgttg ccaaaaggtt ttgtacatcc cagaaataag gataagacgg atggaatttc
     1741 agtattttct gttgggaaac gaaaagtata tccagatttt tatgacagag aacgaaagat
     1801 tgttctagat gcaaaatata aaaaactgga attgactgaa aaaggaatca accgtgagga
     1861 cttattccag ctgatttcct attcttatat tttaaaagct gagaaggctg gactgatttt
     1921 tcctagtatg gagcagtcag taaatagtga aataggaaaa gtagctggct atggagctca
     1981 attgaagaag tggtctattc gaatccctca gaatgcctca tcctatagtg cattttgtaa
     2041 aatgatggaa aattcagaag aaaattttaa agcgattatt gatgaagaag tggggagaaa
     2101 ataatttccc ctctttcttt tatttttaac acaagtaaaa cccgaacgat atattttaat
     2161 tcataaaaaa aattgacaaa aactgtactt tggtttataa taaattaagg aaacgtcgga
     2221 aaaggcgtag tgatgcgaga gcataggtag gtcattacaa aagaaacgag acatcgatat
     2281 gttaaatgaa tttccaattt ttgattacga agatattcaa ttgattccaa ataaatgtgt
     2341 cattaaaagc cgtgcagaag cggatacaag tgtcacttta ggaaatcaca cctttaaact
     2401 acctgttgtg ccagcgaata tgcagacgat tttggatgaa aatgtagcag agcaactggc
     2461 taaaggtggt tacttctaca ttatgcaccg ttttgatgag gcaggacgca ttccttttat
     2521 taaacgaatg cacaatcaag ggctcattgc ttccatatct gtcggtgtta aggactatga
     2581 gtatgatttc gttagacagc tcaagactga tgctcctgag tatatcacta ttgatattgc
     2641 tcatggtcat gcggatagcg tgatttctat gattcaacac atcaagaaag aattgccaga
     2701 tacctttgtc attgctggaa atgtgggaac accagaagct gtgcgtgaat tggaaaatgc
     2761 tggtgcggat gctactaagg tcggaatcgg tcctggtaag gtttgtatca ccaaggttaa
     2821 gactggtttt ggtacaggtg gttggcagtt ggctgctcta cgctggtgtg tcaaggctgc
     2881 gcgtaaaccg attatcgctg atggaggaat tcgtactcac ggtgatattg ccaagtctat
     2941 ccgcttcggt gctagcatga tcatgattgg ttccctcttt gcaggacata ttgaaagtcc
     3001 agggaaaacg attgaagtcg atggtgaaca gttcaaagaa tattatggtt cagcctcaca
     3061 atatcaaaaa ggtgcttaca aaaatgtgga aggcaaacgt atcttgcttc ctgctaaagg
     3121 tcatctgcaa gacactttaa ctgagatgga acaagacctt caaagtgcta tttcttatgc
     3181 aggtggacgt caggttgctg accttaaaca cgttgattat gtgatcgtga aaaactccat
     3241 ctggaatggg gatgcttccc actaagacgg ggtctttgac taaaaaactt gttattagag
     3301 caaatttctg ttataataaa acaagtttcc acccttagtg taatggatat cacgtaagat
     3361 tccggttctt gagatggggg ttcgattccc tcagggtgga tgtaaacatt ctaaaaagcc
     3421 tttaataggg cttttttctt tacactgctc taaatttacc gctaaaactg agaatctctg
     3481 tctaaagaat ttccaaatgc tttccagtcc cgttttagag tgactcaggg ggcttttttt
     3541 gatataataa aaaggactgt tatcagttag aaagaggttg gtatgaaaga attacaaact
     3601 gtactaaaga accattttgc aatcgaattt acagacaaaa agttactgga aactgccttt
     3661 actcatacga gttatgccaa tgagcaccgc ctcttaaaaa tttcacataa tgaacgcttg
     3721 gaatttttag gagacgctgt tctacagtta ttgatttcag aatatctgta taaaaaatat
     3781 cctaaaaagc ctgaaggtga cctatcaaaa ctccgtgcta tgattgtccg tgaggagagt
     3841 ttagctggtt ttgcgcgtga ttgccagttt gaccagttta tcaagttggg taaaggggaa
     3901 gaaaaatctg gtggtcgcaa tcgtgacacc attcttggtg atgcctttga agcctttctt
     3961 ggtgcccttc ttttggataa ggatgtggcc aaggtcaagg aatttatcta tcaagtcatg
     4021 attcctaagg ttgaagcagg cgaatttgag atgattacag actataaaac ccatctccaa
     4081 gagttacttc aggtcaatgg tgatgtggct attcgttatc aggtgatttc tgaaacaggg
     4141 cctgctcacg ataaggtctt tgatgtagaa gttcttgttg aaggtaagag tatcggtcaa
     4201 ggccaaggtc gttctaagaa attagcagag caggaagctg ccaaaaatgc cgttgagaag
     4261 gggctggatt catgtattta aaggaaatcg aaattcaggg gttcaagtct tttgctgata
     4321 agaccaaggt cgtttttgac caaggtgtga cggcagttgt tggaccaaat ggatctggaa
     4381 agtccaatat tacagaaagt ctgcgttggg ctttggggga gtctagtgtc aagagtctcc
     4441 gtgggggcaa gatgccggat gtcatctttg ctggaacaga aagtcgcaaa ccgctcaatt
     4501 atgcttctgt agttgtgact ctggataatc atgacggatt tatcaaggat gcaggtcaag
     4561 aaatcagggt ggaacgccat atctatcgta gtggagatag cgaatacaag attgacggca
     4621 agaaagtccg tctgcgtgat attcatgacc tcttcttgga tactggattg ggacgagatt
     4681 ccttctctat tatttcccaa gggaaggttg aggagatttt taattccaag cctgaggaac
     4741 gacgagctat ttttgaagaa gctgctggag ttttaaaata caagactcgc agaaaagaaa
     4801 ccgagagtaa actgcagcaa actcaggata atctggaccg cttagaggac attatctacg
     4861 agttggataa tcaaatcaag cctcttgaga agcaagctga gaatgcccgt aagtttttag
     4921 acttggaagg acaacgtaag gctatttatt tagacgttct ggttgctcaa atcaaggaaa
     4981 ataaggctga actagagtcg acagaagaag agttggctca ggttcaagaa ctcttgatga
     5041 gttattacca aaagcgtgaa aaattagaag aagaaaatca aactcttaaa aagcaacgcc
     5101 aagatttaca ggctgaaatg gccaaagacc aaggcagttt gatggatttg accagtctga
     5161 ttagtgactt agagagaaaa ttagccctat cgaaactgga atctgagcaa gtagccctca
     5221 atcaacagga agcacaagcc cgtttggcta ctttggagga taagagaagt tcactcagcc
     5281 aagaaaaatc tgataaagaa agctctttag ccctcttgga agaaaatcta gtccaaaata
     5341 atcaaaaact caatcgatta gaggctgaat tgctagcttt ttcagatgat cctgatcaga
     5401 tgattgagct cttacgtgaa cgctttgtgg cccttttaca agaagaagca gatgtctcaa
     5461 accaactgac ccgtattgag aatgagttgg aaaatagtca tcagctttct caaaaacaag
     5521 cagatcaact agaaaagctg aaagaacagt tggctacagc taaagagaag gctggtcagc
     5581 aaaaggctga gcttgaaact gccaaggagc aggttcagaa attattggcc gactatcaag
     5641 tctgtgctaa ggagcaagag gagcagaaaa cttcctatca agctcaacaa agtcaactct
     5701 ttgaccgtct ggatagtctc aaaaacaagc aggctagagc gcaaagtttg gaaaatatcc
     5761 tgagaaatca tagtaacttt tatgcaggtg ttaagagtgt tctccaagag aaagatcgtc
     5821 taggtgggat tattggagca gttagtgagc acctgacctt tgatatgcat tatcaaactg
     5881 ccctagaaat tgcgcttgga gcgagtagtc agcacattat tgtagaagat gagaatgcgg
     5941 caaccaaagc cattgattta ctcaaacgaa acagatctgg tcgtgcaacc ttccttcctt
     6001 tgaccactat caaggctcgt acgatttcta gtcagaacca agatactatt gctgcaagtc
     6061 caggatttct gggcatggca gatgagctgg ttactttcga tactagactg gaagccattt
     6121 tcaagaattt gctagctaca acggccattt ttgataccgt ggagcatgcg cgtgcagcag
     6181 cgcgccaggt tcgttatcag gttcgtatgg tgacattgga cggaacagag ctgcgcacag
     6241 gtggttctta tgcaggtgga gctaatcgcc agaataacag tattttcatc aagccagaac
     6301 tggagcaatt acaaaaagaa attgctgaag aagaagcaag tttgcgttca gatgaagtga
     6361 gtttgaagaa cttgcaagat gagctagcta gattgacaga aagattagaa gccatcaaat
     6421 ctcagggaga gcaggctcgt attcaggagc aaggcttgtc cctcgcctat cagcagacca
     6481 gtcagcaagt tgaagagcta gaaactcttt ggaaactcca agaagaggaa ttaaatcgtc
     6541 tttctgaggg agaatggcaa gcggataagg aaaaatgcca agagcgcctt actacaatca
     6601 ctagtgaaaa gaataatctg gaagctgaga ttgaagagat taagtctaat aaaaacgcca
     6661 tccaagaacg ttatcaaaac ttgcaggaag agctagctca agctcgtcta cttaagacag
     6721 aactgcaagg gcaaaaacgt tatgaagttg ctgatattga acgcttaggt aaggaactag
     6781 ataatcttga tattgagcaa gaagaaattc agcgccttct tcaagaaaag gttgacaatc
     6841 ttgagaaagt tgatacggat ttgctcagtc aacaggctga tgaagccaaa actcaaaaaa
     6901 caaatctcca acaaggtttg attcgcaagc agtttgagtt ggatgatatt gagggtcaac
     6961 tggatgatat tgctagtcat ttggatcagg ctcgccagca gaatgaagag tggattcgca
     7021 agcaaactcg tgctgaagcc aagaaagaaa aggttagcga gcgcttgcgc catctacaaa
     7081 atcaattaac agaccagtac cagattagct atactgaagc actagaaaaa gcgcatgagt
     7141 tggaaaatct caatctggca gagcaagagg tgcaggattt agagaaggct attcgctcac
     7201 tgggacctgt caatttagac gctattgaac agtacgaaga agttcacaac cgtctggatt
     7261 tcctaaatag ccaacgtgat gatattttgt ctgcgaaaaa cctgcttctt gaaaccatca
     7321 cagagatgaa tgatgaggtc aaggaacgct ttaaatcaac ctttgaggcc attcgtgagt
     7381 cctttaaagt gaccttcaag cagatgtttg gtggaggtca ggcagacttg atattgactg
     7441 agggcgacct tttaacagct ggtgtggaga tttctgttca acctccaggt aagaaaatcc
     7501 agtcgcttaa cctcatgagt ggtggtgaaa aagctctatc ggctcttgcc ttgcttttct
     7561 ccattattcg tgtcaagacc attccttttg tcatcttgga tgaggtagag gctgcgctag
     7621 acgaagccaa tgttaaacgt ttcggggatt atctcaaccg ctttgataag gacagtcaat
     7681 ttattgtcgt aacccaccgt aaggggacca tggcagcggc cgattctatc tatggagtga
     7741 ccatgcaaga atcgggtgtt tcaaagattg tttcagttaa gttaaaagat ttagaaagta
     7801 ttgaaggatg acaattaaac tagtagcaac ggatatggac ggaaccttcc tagatgggaa
     7861 tggacgcttt gatatggatc gtctcaagtc tctcttggct tcctacaagg aaaaagggat
     7921 ttactttgcg gtggcttcgg gtcggggatt tctgtctctg gaaaaattat ttgctggtgt
     7981 tcgtgatgac attattttca tcgcggaaaa tggcagtttg gtagagtatc aaggtcagga
     8041 cttgtatgaa gcgactatgt ctcgtgactt ttatctggca acttttgaaa agctgaaaac
     8101 gtcaccttat gtagatatca ataaactgct cttgacgggt aagaagggtt catatgttct
     8161 agatacggtt gatgagacct atttgaaagt gagtcagcat tataatgaaa atatccaaaa
     8221 agtagcgagt ttggaagata tcacagatga cattttcaaa tttacaacca acttcacaga
     8281 agaaacgctg gaagctggtg aagcttgggt caatgataat gtccctggtg tcaaggctat
     8341 gacaactggc tttgaatcta ttgatattgt tctggactat gtcgataagg gtgtagctat
     8401 tgttgaatta gctaaaaaac ttggtatcac aatggatcag gtcatggctt ttggagacaa
     8461 tcttaatgac ttacatatga tgcaggttgt gggacatcct gtagctcctg aaaatgcacg
     8521 acctgaaatt ttagaattag caaagactgt gattggtcac cataaggaac ggtcggttat
     8581 agcttatatg gagggcttat aatggcagat ataaaattga ttgcattgga cttggacggg
     8641 accttgctga ctactgataa aaggctgacg gatcgtacca aggaaacctt gaaagctgcg
     8701 cgtgatcgtg gtatcaaggt cgtattgaca actggtcgtc ccttaaaagc catggatttc
     8761 tttctccatg agttagggac tgacggtcag gaagatgagt ataccattac ttttaatggt
     8821 ggattagttc agaaaaatac aggagaaatc cttgataaaa cagtcttttc atatgatgat
     8881 gtggcacgtt tgtatgaaga aacagagaaa ttatcactgc ctctcgatgc catctcagaa
     8941 ggaacagttt atcaaatcca atcggaccaa gaaagtcttt atgccaaatt caatccagct
     9001 ttgacctttg ttccagtgga ctttgaagac ttatctagtc aaatgaccta caacaaatgc
     9061 gtgactgcct ttgctcaaga acccttggat gcagccattc agaagatttc tccagaattg
     9121 tttgaccaat atgaaatctt taaatcacgt gaaatgttgc tagaatggtc accaaagaat
     9181 gttcataaag caacaggttt ggcaaaacta atcagccatc ttggaatcga ccaaagtcaa
     9241 gtgatggctt gtggtgacga ggccaatgac ctctctatga ttgaatgggc aggtcttggt
     9301 gttgctatgc aaaacgctgt tcctgaagta aaggcagccg caaatgtagt gacgccgatg
     9361 accaacgatg aggaagctgt cgcctgggct atcgaagaat atgtgctaaa ggagaactaa
     9421 gatatgggat tgtttgaccg tctattcgga aaaaaagaag aacctaaaat cgaagaagtt
     9481 gtaaaagaag ctctggaaaa tcttgatttg tctgaagatg ttgatcctac cttcacagaa
     9541 gttgaggaag tttctcagga agaagcagag gttgaaattg ttgaacaagc tgtgttccaa
     9601 gaagaggaaa tccaagacac agttgaagaa agtctggatt tagagccagt tatagaagtt
     9661 tctcaaaaag aagtcaaaga atttccacac tcagaagaag ggaatactga gtttctagag
     9721 actgtagaag aaaataattc tgaaattctt gaagcagaaa ggcctcaaac agaagaaact
     9781 gttcaggaaa aatatgaccg cagtcttaag aaaactcgca caggtttcgg tgcccgcttg
     9841 aatgccttct ttgctaactt ccgctctgtt gacgaagaat ttttcgagga actggaagaa
     9901 ctgttgatta tgagtgatgt tggtgtccaa gtcgcttcta acttaacgga ggaactacgt
     9961 tacgaagcca agcttgaaaa tgccaagaaa cctgatgcac ttcgtcgtgt catcattgag
    10021 aaattggttg agctttatga aaaggatggt agctacgatg aaagcatcca cttccaagat
    10081 aacttgacag ttatgctctt tgttggtgtg aatggtgttg ggaaaacaac ttctatcgga
    10141 aaactagctc accgctacaa acgagctggt aagaaggtca tgctggttgc agcagatacc
    10201 ttccgtgcgg gtgcagtagc tcagctagct gaatggggcc gacgagtaga tgttccagta
    10261 gtaactggac ctgaaaaagc tgatccagcc agcgtggtct ttgatggtat ggaacgtgcc
    10321 gtggctgaag gtatcgatat tctcatgatt gatactgctg gtcgtctgca aaataaggat
    10381 aaccttatgg ctgagttgga aaagattggt cgtattatca aacgtgttgt gccagaagca
    10441 ccacatgaaa ccttcttggc acttgatgca tcaacaggtc aaaatgccct agtacaggcc
    10501 aaagaatttt cgaaaatcac acctttaacg ggaattgttt tgactaagat tgatggaact
    10561 gctcgaggag gtgtggttct agccattcgt gaagaactca atattcctgt aaaattgatt
    10621 ggttttggtg aaaaaatcga tgatattgga gagtttaact cagaaaactt catgaaaggt
    10681 ctcttggaag gtttaatcta atcagaagca aaaatcctgc aaggcataaa cttgcaggaa
    10741 atttttttat tctaagcgac catcttgacg ataggcgata tctggttgcc aagtccattt
    10801 ggcaccgaat ttttcaagta ggtcaaagct ggcttgaggt cccatgcttc cagctttata
    10861 gtcatgaagt ggggcaccat tttcatccca gagctcttca atacggtcaa tcaacttcca
    10921 tgatgcacca acttcatccc agtggctaaa gttagttgag ttgttattta ggacatcata
    10981 aatcaatttc tcgtatggtt ctggagaagc accagttgca gtcgcatctg tacggtaatc
    11041 aagtgagtta ggagccaagt taaattcttc tcctacttgc ttcccattta ggctaagaga
    11101 gaagccttct gttggttgaa tatagatggt caaaatattt ggagcaagtg gttctccaaa
    11161 gatagaatcc atttgtttaa agacgatgtt gacatgagtt cctttttcag tcagtcgttt
    11221 acctgtacgg aaaaagaaag gaacactacg gaatcgatcg ctgtctacaa agaaggcacc
    11281 agatgtaaag gtttcagttg ttgattctgg attcacattt ggctcgctac gataagagat
    11341 gtatttcatg ccatcaatct taccagagcg gtattgccca cggataaagt gttctttgag
    11401 ttcttcatca gttggatgat agaggttttt aaagacctta atcttttcag cacgaatctc
    11461 gtcttttgtg aagcttgctg gtttgtccat ggcgaggagc gaaagaagtt gtagagtgtg
    11521 gttttggacc atgtcacgga gggcaccgga ttggtcatag tagccaccac gttcttctac
    11581 acccaagcgc tccgcaaagg taatttgaac attgtcgata aaatccttgt tccaaacgtt
    11641 ttcaaaaatc aagtttgcaa agcgaactgc aaagatgctt tggatcattt ccttaccaag
    11701 ataatggtcg atacggaaaa tttgttcttc gtcaaatgtt gctaggagtt cgtcattcaa
    11761 cttgcttgca gttgcgtaat ctgtaccaaa tggtttttca acgatcaagc gctcaaaacc
    11821 tttgccatcg acaatgtttt cagatttgag gtgtttggca atggttccaa agaactgagg
    11881 tgccatagac aagaagaaga gcttattgtg ttcagcttgg tatttttcat taagctcagc
    11941 ttgtaattga cgcaaagcaa tataatgttc cgaatcattg acatcatggc tttgatagta
    12001 gaagtggcta gcaaattctt gggcttgctc ggtactatct gccaaatcaa ggatggactc
    12061 gacaactaca gattcaaaat attccttact ccaaggtcta cgggcagttc caataacggc
    12121 aaagtgcttg gaaagattgc cggatttata tagtctaaaa agggaagggt agagcttgcg
    12181 tttagccagg tctccactcg caccgaaaat tgtaacaata accttagatg acatctagct
    12241 acctaatttc tatttttatt cctagtcttg ctaggtatga ggaaacctca tttcataaac
    12301 ttaattctaa cataattttc cgaaaaatca aaaattccaa tacgaagtag aaaaactgca
    12361 aggaaatcct tacagtttga gtttagacgt ttaagacctt atctaagaac tctttcagac
    12421 gagggtgttg tgggttatca aagatttggt caggtgttcc gtcttcaagg aactcgccat
    12481 ctgcagtaaa gataacgcgg ttggcaacct gacgagcaaa tcccatctca tgggttacga
    12541 taatcatggt cataccttgc tcagccaatt ccttcataac gttaagtacg tctccaacca
    12601 tctcagggtc aagggcagaa gttggttcat cgaagagcat gatgtctgga ttcattgcta
    12661 ggccacgagc gatggccaca cgttgttttt gaccacctga taggctatct ggattggcat
    12721 tagctttatc tgctagtcca accttttcaa gcaactccat tcccaattcc tcagcttctt
    12781 ccttagtcat caacttgtgc tcaataggag caaaggtgat gttgtccaat acagacatat
    12841 gagggaagag gttgaagtgt tggaatacca tgccgatatt ttcacggacg tggtcaacat
    12901 tggttgtttt ttcagttaaa tcatagccgt tcacagtgat gtgaccgcta gtgacttctt
    12961 ctaaaagatt gaggctacgg aggaaagttg acttaccaga acctgaagga ccgatgatac
    13021 aaacaacatc tccttcatag aacttagtcg taattccttt taggacttca ttttttccat
    13081 agtgcttgtg taaatcattt acatcaattt ttaattttgc cattaacgaa tcctcttttc
    13141 taagcgtttc gctagtctag tcaaaagtgt gataattaca agatagaaga tagcaaggat
    13201 tgcatacatc ttgaaacttt ggtagttacg agcaatgata atcttaccag tttggaagag
    13261 ttcaaccaaa ccgatagcag atacgatagt tgtatcttta agagcgataa cgaattggtt
    13321 gacaaagttt ggcaacatca atttagttgc ttgtggcaag ataatcttac gcatggtttt
    13381 tccataagag atacccaagc ttcggctggc ttccatttgg ccaactggaa cggcctgaat
    13441 accaccacga acgatttcag cgatataagc agccgcattg agtgagaggg caatggttcc
    13501 agctacaaag tcgttaattg ggctttgttg gcctgtgata gactcgatga agtttggaat
    13561 tccccagaag atgaaggctg caagaatcat caatggaata ccacgaataa cgtcaacgaa
    13621 aatctcagag atgacgcgaa gagatttgta tgggctaacg ctaaacatac cgaagataat
    13681 tccgatgaca atggcaatag caaatgagat aagagctaga gcaagagtga taccaagacc
    13741 gctaaggagt tgtttgtagt tgttttgaag caagccccag agcgttgttt cgtcaacagt
    13801 acttgttgaa gcagttgaag attcgctagc taggtatttg tcaagaatct tttggaattc
    13861 accgtttgct ttaaggtttg caagtccgtt gttgaacatt tcaatcagtt ctggatttgc
    13921 tcctttttta acggcaaagg ctgtttcacc gattggagtt ccagagattg gagttttcaa
    13981 tttttgacct tggctgatag aatatttgag aacaggttca tcatccataa cggcatcaat
    14041 ggcaccagtg tttaaactgt catacattga agaaccatca gcaaaggttt tgattttgta
    14101 accgtatttg ctttgatttt ctgttaggaa ggtttgagaa gcagttccgt ttttaacacc
    14161 gactgtcttt ccttttagat cttcataaga agcaatattg cttgattctt tgacaccaag
    14221 aatggtatta gcagtgtagt atgattctga gaagtcaaaa gttgccttac gagcatctgt
    14281 gacagacatc ccagcaatga taccatcggc ttgaccagct tggacagcac tgatagcagc
    14341 atcaaaacca gggttggtga tttcaatttc aaaaccttgg tctttagcga ttgccttaat
    14401 caattccata tcaataccag tgtattggtt gcttgaattt tggaaaacaa aaggtgcaaa
    14461 agaagaatcg ctggcaatga tatatttagc cttaacagga atagctttta atcctgcgag
    14521 agtagttgta gttggcactg ctgaagatga tgaagcagtc catttcttga taattttatc
    14581 aagactacca tcttttttca tttcagccaa ggcttggtta aattcagtaa ccaggtgctc
    14641 gtatttactt ccttttttca caccgaaagc aaaacttcct acagcttcac catccatttc
    14701 aatatggaga tcttgacctt ggttaatggc atattcgata acaggtttgt catccatcat
    14761 ggcatcgatg gcaccagcac tcaagctgtt gttcattaaa tcaccagtgt caaatgtttt
    14821 aatagtaaag ccgtatttat ctttgattgt ttcaaggaaa cgttgagcgg cagttccgtt
    14881 tttaacacca acggttttgc cagttaattg gtcgtacttg ctaattttgt gtgactttgt
    14941 agtagcaatg acaacttttg tatcatagta agtatcagac atggtgaaga ctttttcacg
    15001 ttctttagtc tttgtcatcc ctgccatgat agcgtcggct tgcccagctt gaaccgcatt
    15061 gactgctgcg tcaaatccag gataggacat ctgaatgttc cagcctttaa tctcagcgac
    15121 tttgttaata atgtcaacat caattccttt ataagtttga tctgaatctt taaactcaaa
    15181 aggtgcatag gcggtatcag aaacaatctt aatcgtttct gctttggcaa tacctaatga
    15241 gaaaattggg aataaaatta gcaaaaatgc tagaaatttt ttcttcattt ttagtctcct
    15301 tttccgaata ttctctcatt atatcagaaa aagtaaaaaa aggcaaattg ttatattttt
    15361 cgtgtcagaa aatataacat tgatttttcc ttttctttct tgaattgcta ttaaaaa
//
LOCUS       DAVLVF010000035        15308 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVLVF010000035 DAVLVF010000000
VERSION     DAVLVF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15308
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.035"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(261..1868)
                     /locus_tag="VYN66_001145"
     CDS             complement(261..1868)
                     /locus_tag="VYN66_001145"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV1495174.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAAAMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDTEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2258..2845)
                     /locus_tag="VYN66_001146"
     CDS             complement(2258..2845)
                     /locus_tag="VYN66_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV1495175.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2973..3422)
                     /locus_tag="VYN66_001147"
     CDS             complement(2973..3422)
                     /locus_tag="VYN66_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1495176.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3427..3816)
                     /locus_tag="VYN66_001148"
     CDS             complement(3427..3816)
                     /locus_tag="VYN66_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV1495177.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQTLLADRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3844..4470)
                     /locus_tag="VYN66_001149"
     CDS             complement(3844..4470)
                     /locus_tag="VYN66_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1495178.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPDILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4484..5047)
                     /locus_tag="VYN66_001150"
     CDS             complement(4484..5047)
                     /locus_tag="VYN66_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1495179.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5212..5446)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5500..6003)
                     /locus_tag="VYN66_001151"
     CDS             complement(5500..6003)
                     /locus_tag="VYN66_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV1495180.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6196..7026)
                     /locus_tag="VYN66_001152"
     CDS             complement(6196..7026)
                     /locus_tag="VYN66_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1495181.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(7011..8693)
                     /locus_tag="VYN66_001153"
     CDS             complement(7011..8693)
                     /locus_tag="VYN66_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495182.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LISDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8795..9052)
                     /locus_tag="VYN66_001154"
     CDS             complement(8795..9052)
                     /locus_tag="VYN66_001154"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495183.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(9059..9607)
                     /locus_tag="VYN66_001155"
     CDS             complement(9059..9607)
                     /locus_tag="VYN66_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV1495184.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9622..10470)
                     /locus_tag="VYN66_001156"
     CDS             complement(9622..10470)
                     /locus_tag="VYN66_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV1495185.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10658..12007
                     /gene="trkA"
                     /locus_tag="VYN66_001157"
     CDS             10658..12007
                     /gene="trkA"
                     /locus_tag="VYN66_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV1495186.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            12011..13450
                     /locus_tag="VYN66_001158"
     CDS             12011..13450
                     /locus_tag="VYN66_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1495187.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKMVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13575..13832)
                     /locus_tag="VYN66_001159"
     CDS             complement(13575..13832)
                     /locus_tag="VYN66_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEV1495188.1"
                     /translation="MNEGAQLAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYRE
                     MKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
     gene            14592..14762
                     /locus_tag="VYN66_001160"
     CDS             14592..14762
                     /locus_tag="VYN66_001160"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1495189.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEETG"
BASE COUNT         4590 a         3427 c         2765 g         4526 t
ORIGIN      
        1 tgttcacatc ttatttcaat tcatgataca atccagttcc taaaactttg ctagaaatta
       61 atttggcttt ccgaattaat ttgttcagat tttatttcat ttcaatatac tgataaatcc
      121 attcactagc acagggcaag agggagtcgc tattgatttc aacggtagca ggtacataca
      181 gaggactcct acctcagata tactgcaata tcatcgtatg caaaaaacct ctgagatttt
      241 tctcaaaggt tctgattttg ctaattgctg ttctcaaccg ctgcagtaac aaaggcagtg
      301 tagagttctt ctggtcggtt tggacggctt gacagttcag ggtgatactg acaagctaca
      361 aagaatttat tttcaggaat ttccacgatt tctaccaaac gattgtctgg agaaactcct
      421 gaaaagacaa aacctgctgc ctcaaactgc tcacggaagg cattattaaa ctcataacgg
      481 tgacggtgac ggcgttgcac cacttcttga ttgtgataag cagcagcagc cttagagcca
      541 cgtttcaact tagacggata aagtcccaaa cgaagggttc cacccatatc ctcaatatca
      601 atctgatcac gcatgatatc aatgataggg tattttgttt ctggtgcaag ctctgcagaa
      661 ttggcacctt caagacctaa aacgtgacga gcaaactcga tacatgtcaa ctgcattccc
      721 aagcagactc ccaacattgg aacatcattt tcacgcgcat agcggatggc ttggattttc
      781 ccttctgtac cacgttgacc aaaaccacct ggtacgatga tcccgtccgc atcagacaag
      841 agttctgcta cattctctgc tgtcacatca ttggcattga cccaattgat tttaacttct
      901 gtatcattga catagccaga gtgtttcaag gcttcgacca ctgagatata ggcatcttgc
      961 aactccacat acttaccaac aagggaaatc ttaacttgtt tcttgaggtt catgaccttg
     1021 tccaccatgg ctgaccattc tgtcatagcc gctgctggtg cgtctaattt caaatgatca
     1081 caaacaattt ggtccatccc ttgtgcctgc aagttcagtg gaatttggta aaggtgttca
     1141 acatccaacg attcgataac ggcttctggt gccacatcac agaactgggc cagtttattt
     1201 ttaattcctt gaccagctgg ctcttctgta cgaataacca acatatttgg ttggattccc
     1261 aagccacgca attctttgac agagtgttgg gttggtttgg ttttcatttc accagcagcc
     1321 ttgaggtaag gaagcaaggt tgtatggata tacatgacat tatccgcacc cacatctgcc
     1381 ttcatctgac gaagagcctc taggaatggc aaggactcga tatctcctac tgttccacca
     1441 acctctgtga taatgacatc agagtcggtc gttagagcgg cacgcttgat tttttctttc
     1501 aaagcatctg tgatatgagg aatgacttga acagttgccc caaggtattc tccacggcgt
     1561 tctttacgaa gaacttcact gtaaattttc ccagttgtca cgttggaata tttgttgaga
     1621 ttgatatcga taaaacgttc atagtgaccc aagtccaaat ctgtctcagc tccgtcatct
     1681 gtcacaaaaa cttccccgtg ctggtaagga ctcatggttc ccggatcaat attgatataa
     1741 gggtcaaact tttgaatggt tactttgaga ccacgatttt tcaagagacg gcctagactc
     1801 gctgccacaa tccctttccc aatagacgat accacaccac cagttacaaa aatatatttc
     1861 gtagacatag attcctcttt ctaaaatgct caaggtcttg ttaactacga ggggatatag
     1921 aaaacaagac cctactaata agaataatct gggacgactg caccagattc acaactaata
     1981 ctcttcgaaa atctcttcaa accgcgtcaa cgtcgccttg ccgtagatat gtgttcctga
     2041 ctttgtcagt cttatctaca acctcaaaac agtgttttga gcagcctacg gctagtttcc
     2101 tagcttgctc tttgattttc attgagtata aaatacaaga agataacttc ttgaaaaaac
     2161 aaaaatagct ccctaagaac tagggagccc cgacctctaa aagaggtgcc caaacaatat
     2221 gatacctaaa aataggataa ttgtcaatag ctaactctta ttcctcgctg gtttcagcgt
     2281 cgtcatctga aaactcgtcg tcgtcttcgt tgagatccac atcttctccc aagtcatctg
     2341 gagcgatttc gttgatttct gcatcataag cttccacttc atttttttca tcatctggat
     2401 tttcatcatc gtatgaaaga gctggatctg cttcgtatgc atcttcgtct tctggatcat
     2461 ctgcattgta gtcaatggca tctgaatcac catccataaa ggcattgaca cgttttttct
     2521 tagcttttgg tgctacttca tcgtcgtcat tttcttcaag agcgatgatt tcttcgtcga
     2581 tttcgtccac accataccat gaacgaagac cccatttgtt gtccccaagt gagatgaagc
     2641 taccgtcaaa gttcaactct gtgtagaaca gaggcaaagc ttcgcggata tcgctgtttg
     2701 atgttccaag gtagttttga atttcgttta caagatcgct aaaatgcatc tcatgatcgc
     2761 gaccacgaag ttctaatata gcacgcgcta cctcaatcat agatagttca cttttttctt
     2821 gcccagcaaa tacttctaat tccaaagcgt ttctcctcat ttatactact atcgccagag
     2881 cgaacagact ctgacctcat tttatcattt actctttatt ttacgataat tttgcggaat
     2941 agtcaaaggt taagggggag aaagtggcag gattagacta attccaatat aaaactcctt
     3001 cctttttctg ttgctccatt ttccacaaat ccaagcgact tgaaacacct cctagaagca
     3061 tgattgtagg tgtagatttc cttgactctc aattctttcc atccttttac tcgagccaat
     3121 tcaatcaaag cacttagaat cttttttcca agtcctcgat gttggtaagc ggaattccca
     3181 atcacaatgg ggagattatc ctgagatagt gtaatatccc caattggaaa ccattctccc
     3241 ttctccttga cttcaatcca aaaaagctca ccatgccgat ccaaatagga atacatggct
     3301 tccaaggtcg cttgactgta aggaagcttc accccatcta cgaggtaaac caagttcaca
     3361 tcctgatacc aagcaaaagc ttcctcacaa tgattgacct tataataagg aacaaaacac
     3421 aatgcactac cttttcaaca aaagagttgc tgcttgatta aaaccatcac accagttata
     3481 ccattttgct tcatacccat cttgagctag gatacgatct tctaaatcaa aaacagagta
     3541 aatctttctt tcctcgcaag cttgcgcata gagatgatat agttcatcac caccatctct
     3601 atcccactca gcagaaatcg tatcccgatc tgccaataaa gtctgataag ccctgtgatg
     3661 cccatctgta atcagcaaac aatctccaaa ggcaagaata ctgattggat cgacttggat
     3721 tgtttctgcc gactggtaaa gcatctgaat atcttgcaac ttcttttctg ataaatatag
     3781 ttgagtcgga tgaagatctg ctatgttgac tttcatttct ttctcctcaa gggaattcga
     3841 tactcacttc tgtttgcctt taaatcgcca ttggaagcgg agcttgtcat agaagggaaa
     3901 ctcgataaac aggactccca agcccacaca gagactggca aggacgtctg atgggtaatg
     3961 aactcccaga tagactcttg ataccagcac actgactagg tagaggccaa ggacgatttg
     4021 tacgattttt ctccagactg gatctttaat ccgctgacta agaataacaa tcaaagtacc
     4081 taccatcaag gttacagcca gagaatgccc acttgggaag gaaaatccct tctcctccac
     4141 cagatgtaag atatctggcc gtgggcgctg gtagatattt ttaaaggtca cgattaaaag
     4201 acctgccaaa gccagatttc ccagcatgaa gaaactttct atcttccatc gcttacgata
     4261 aaagacaaaa gctgtaatga caacccaagt gataatcact gggatatcaa tcagacgtgt
     4321 gagggctcga aaaagaatag tcaaataatc tggtaagtct cctcgaatgg cagtctgaat
     4381 cgattggtca aaattgacca gcgtttcagg gtaaaatttg accatgtagc caagaataac
     4441 gaaaagtaaa agggcaaaac tgcccttcat taaaaatgtt tgtttatctc tcataatgtt
     4501 ttaaggttgg tttcaagaga acatacaaca accagaatga aacggaaaag ataacacctt
     4561 caatcaagtt aaaaggtaat accatggtca ttaggtagtt ggaaagtccc aaaatttttc
     4621 caatatcaaa gttagcaaac ttagcgtaca aaggaacagc ataaacatag ttgagaacca
     4681 acatggccaa ggttaaacca atagttccag ctagagagcc tagtaggaaa cgaagggttg
     4741 tccgttcctt tttccaaatc aaagcaaata cgatgacaaa aactcccaaa gctacgatat
     4801 tcatcggcaa accaatgtaa gtattcactc cttggctgtt aagaagcaac ttcaagagtg
     4861 agcgaagcaa gagcactcct agagcagcag gcaaatccat gaccaccaga cccacaagga
     4921 ctggcaagat actaaattcg atcttgagga aagatgccgc tggtaaaagc ggaaaatcaa
     4981 agtacatcag cacaaatgag atggctgata gaattgcaat ggtcgaaagt cgacgtgtgt
     5041 ttgtcataac aggttcctcc aattttctat aaaatcagaa gaagttggaa aggattcctc
     5101 tatctattct cactttttat atcccaaaag ttccctctta ctctattaaa gaaaaacaaa
     5161 gcaagtggtt acaatccggc tataaatcta tcaaaacaga caaagctatt ctttcgtctt
     5221 ctcccatcca gactatactg tcggttgtgg aatctcacca catcagcttg cgctcgcgga
     5281 cttctttaaa gggaaagaaa tagagatttt tcttttagac taggcgacaa gctgtaggca
     5341 taacttctgg ttaggcaaag cttgtcaaca aagtataaaa ggaaaagatc atttcctagt
     5401 taaagtcacc gccggtcggg aatcacaccc tgccctgaag actcttttat cataacaaaa
     5461 aacgcttgca agcgcaagca ttttgttcat tttcattttt tatttcaatt tatccacttc
     5521 ataggtatgg acgagctcaa gacctgcaaa gttctgctgg cgaagagcct catagacaat
     5581 catgcagacg gtattagaca cattgagact gcggacatgt tcatcattca tgggaatacg
     5641 gagagccttc tcaggatgtt ctcgcataaa atcttcaggt aagcccttgt cttcacgtcc
     5701 aaagagaaaa taatggtcct cgtcagtcga taaatccacc tcagaataga ttttttccgc
     5761 gaatttcgaa atcagataga gttttccctt catctgagat atgaaatctt ctaaactctc
     5821 gtaaaaataa atctctagct tatcccagta atccaatcca gcccgcttca tcttgcggtc
     5881 atcaatagga aagcccatag gcttgatgat gtggagggga gaattggtcg cagcgcaagt
     5941 acgcgcgata ttgcctgtat tttgtggaat ttgaggttca aataatacaa tgtgatttgt
     6001 catgacttgc ttcctttcac cattgcaaaa aaatagccac actgcccgga gtcaagctca
     6061 gcaaacagcg tggttaaggc atcgttaact tacctcacaa caggtttgaa gtaaatcagc
     6121 gaaactactt tcttagtata acactttcag aatcattgtc aatagaaatg acttgatttt
     6181 ttcaattttt tcaagctatt tccaagggtt gtaaaatcgt ccctgattct gcaagataag
     6241 tagtaaacta actactaaaa acaaggttgc caagagcaag gtaatatagt ctcctttttt
     6301 caaggcctga taactatacc atgtgcgttt tttctctttc ccaaagcggc gaagctccat
     6361 ggcagtcgca atggtatcaa tgcgttctag cgagctaaaa atcaagggcg taataatgag
     6421 cagattgcct ttgattcgtt gcataagaga agctttcttg gataattcca tcccacgcgc
     6481 ctcctgagac atcttgatag taaagaattc ttcctgcaaa tctggaatat agcgcaaggt
     6541 caggctgaca gaataagcaa tcttataggg cacaccaatt tgatttaaac tggaagcaaa
     6601 ctgactagga tgggttgtca tcaaaaagat aatagccaga ggaatggtgc aaagatactt
     6661 aatggccaaa tttagcagat aaaagagctc ctggctggtt agagtgtaga caccgattcc
     6721 ctgccaaatc acacttctct ctccataaag tccaacccca tactcgggag aaaagagata
     6781 gaccatcaaa acgtttaaaa cggcaaatac cgtcgcaaaa acggctacaa aggaaacatc
     6841 tttaaagcga atttctgata aatagaggag aaagactgaa aagatggcaa tcagcaagag
     6901 cattctggta tcatagctaa tcatggccgc caatgatacc agaatgaaaa agagaagttt
     6961 cccagctcct gacaagcgat gaatcacagt atctctatgc tggtaaccga ttaatttagc
     7021 ttgcatccct ctctcctttc tttgtaaaat gccgttaaat ccagtggatc cacatctagt
     7081 ttcttagcca agttaaagat ggaggtttct tttagattgg cttttactaa cagctcagga
     7141 tcgctcaaca gactggctgg aacagtatcg gcaatcaatt ctccatccac catgacaagg
     7201 acccggtctg aataatccag catcaattgc atatcatggg taatcatgac aatggtatgc
     7261 cctttttgat gtaactcttc gagaaattcc ataatctcag tatagttctt ctgatcttga
     7321 cctgcagtcg gttcatctag gagaataatt tcagctccta agaccaaaat tgaagcaatg
     7381 gtgacacgtt ttttctgacc aaatgacagg gcagaaatag gccaattacg gaattcataa
     7441 agtccacaga ttttcaaggt ttcatagact ctcgtttcaa tttccttctc atccacacct
     7501 cgcaaacgga gccctagagc cacctcatca aaaatcatat tggttgaaat catttgatta
     7561 ggattttgta gcacatagcc tactcgttcc gcccgctctg caacagaatc gccttttata
     7621 tcctgttttt cccaaagata gcgtccttcc gtctgaataa agctacttat agccttggct
     7681 agagttgatt tccctgctcc attttttccg acaatagcaa tcttttcacc ctttttaata
     7741 tctaaatgta gggattttaa aatcggtcta tcatcataag agaaagacac gtcctctaat
     7801 ttaaagagtg actgcaattc tggagtttct tttgccagtt cattctgcag ctgaacctga
     7861 ccttttgaga tagacatgtt atccagattc gctaactgtt cttccttgac taagtccaca
     7921 cctaattgac ggagagtcgt tagataaagg ggttctcgaa ttccattttg agtcaataaa
     7981 tcagtcgcaa gcaactggtc agggctccca ttaaaaagga tacgaccatc gcttatcaag
     8041 acaatccgat ccacagggcg atgcagaaca tcctccaaac ggtgctcgat aatcagagtc
     8101 gtcgtcccct cttccttatg aatctggtca atcaattcga taatatcctg acctgacttg
     8161 ggatctagat tggcgagtgg ctcatcaaac aagagaatcg gactttcatc aatcaagaca
     8221 ccagccagac tgactcgctg cttttgtcca cctgacaaat cctgaggacg ctgatccagt
     8281 aaaggaagaa ggtccagctt ttcagcccat ttataaacac gacctttcat ctcatctagg
     8341 gctgtcacat cattttccag agcaaacgcc aaatcttctg ccacagacaa gccaataaac
     8401 tgcccatctg tatcctgcaa aactgtgcta accagatgag acttatcata gatgctcata
     8461 tcaaaggcta cttgaccctt tatcaaaaat tctccatatg tctgaccctt gtaaatattg
     8521 ggaataatcc cattcaaaca ctgacccaag gtagatttac ctgacccaga tggtccaaca
     8581 attaagactt tctctccctt gtaaatggtc aagtctatcc cttgcaaggt cggttcttgt
     8641 tgtgtttcat accggaaaga gaaatccttc cactcaatta tagcttcttt catcttactc
     8701 tcttcattcg cttcttagac ttctatttta tcataaatca agcccttctt gcagtctctt
     8761 ctcttaaaat cttagcgcca aaaagattcc tatcctagct tacttgccta actaatctat
     8821 aaacatcgaa aaagactagt tgcccagcct tccccatcat tttatactct tcgaaaatct
     8881 cttcaaacca cgtcagcttc gccttgccgt aggtatggtt actgacttcg tcagtttcat
     8941 ctacaacctc aaaaccatgt tttgagctga cttcgtcagt tctatccaca atctcaaaac
     9001 actgttttga gcaacctgcg gctaacttcc tagtttgctc tttgattttc attgagtatt
     9061 agtccttttt caaacttcct gcacgagttt gggttcctgc ataggcaagt aagagaagag
     9121 ttcctgcaat agctacagat acaccattgg caattcccgc aacaatccct tgtgcaaata
     9181 ctttttctgc cgcttcttga taaatcacaa catctccaag tggtgccaag acaccccaaa
     9241 caagggcatt tgcaagtagt tgaatgaggt taaaaataag aatatctttc cagtcaaaaa
     9301 caccattgat cactcgaacg tactttctaa aaagtcccac aactagacca aagagtccgc
     9361 tagcgataat ccaagtccac catagaccat aaccagcaag agagtccttg attgcatgac
     9421 caatcaaccc gacaagcaaa ccgataatcg gtccaaaaat aatagaaagt agcgcttgta
     9481 ccgcatactg aagctggatg cttgtatttg gaacaggggt tggaatgttg atcatcccga
     9541 tgacgacaaa gagggcagcg ccaattccga cagcaacaac ttgtttaatt gtaaatttga
     9601 tttccatact attctcctat tttatccttc tattttcttt atttcaatgg tccaagatga
     9661 accgacacct acattatagg ccttggcaaa ggaaccttgg ttgatagcca aacctaaacg
     9721 atagagagag ttgatgtaaa ggatgggttg cccaattctc acatctgcaa atgatttgcc
     9781 atagacaacc tgattttgat agaccagcat atcagcatga tagatggtca cttcaaaacg
     9841 atcaccaaat tctggttcca gcttgtaaaa ttcttcccgt gtgatagagg tccaaagcga
     9901 accgaaacgc acatccagaa tatcaatggc tcccttcacc agatgatctt ctatgatggt
     9961 cgctacgact ggaagctcta caatctgttc cacactgagc tctggcccta cttcctcaaa
    10021 agtaatgtga ccactggcca gtttagcacc agtataggca tagacatcac gaccgtggaa
    10081 ggtataagaa tgctctgtgt tttgacgcct attggccacc tcagaaatct cacgaatggc
    10141 tacaatgcca acgtgtttct tgataaagga aagcgtccca ttatctggcg tgacaatgta
    10201 ttgatttttt gcagtcttgg caactacact cttacgtttc gaaccgacac ctggatcgac
    10261 aaccgataca aacgtcgttc cctcaggcca gtaatccacc gtctgaaaga gacgatagct
    10321 cccctcaaaa atattataag gcgtgatatc gtgcgtcaag tgatggattt ttaaggttgg
    10381 agactcttct aaagccactc caatcatagc cgacaccgca ccatcaacca gaccaaagtc
    10441 tgattgtaat accagtaaat tattgttcat tttatctcct tgtatatcct ttatcatacc
    10501 atatttcaga gaaagtcgct aatagctatt tcaattggtt agggtatagt tttaccttat
    10561 agcgaatttt cactaaaagc tcttgttatt agctagtagg tgcatttttt cttgaaaaag
    10621 ggtatgatag ttacatcatg aaaaagtagg ttttattatg aaaattatcc ttgtcggagg
    10681 gggaaaagtt ggttttgccc tctgtcgctc cttggttgca gaaaagcatg atgttttgct
    10741 gattgagcaa gacgaagctg ttctcaatca tattgtcagt cgctttgata tcattggtat
    10801 ccttggtaac ggggccgatt ttgccattct tgagcaagcc agcgtccaag attgtgatat
    10861 ctttatcgcc ctgactgagc acgatgaagt caacatgatt gcagcagttc tagccaagaa
    10921 aatgggagct aaagaaacta tcgttcgggt gcggaaccct gaatattcta actcttattt
    10981 caaggaaaag aatattctcg gtttttctct tatcgttaat cctgagctct tggctgcccg
    11041 cgctatcgcg aatatcattg acttccccaa cgccctgtct gtcgaacgct ttgctggtgg
    11101 acgcgttagc ctcatggaat ttgtcgtcaa gtccaccagc ggtctttgcc aaatgcccat
    11161 ttctgatttt cgtaaaaaat ttggtaatgt cattgtctgt gcgatagaga gggatcatca
    11221 aattatcatt ccaagcggtg acatgactgt acaggataaa gatagaatct ttgtcactgg
    11281 taaccgtgtc gatatgatac tcttccataa ttattttaaa tcacgcgccg tgaagagcct
    11341 tctcatcgtt ggagcaggta gaattgccta ttatctactt ggtattctca aagatagtcg
    11401 tatcgataca aaagtcattg aaatcaatcc tgaaatcgcc agcttcttta gcgagaaatt
    11461 cccaaatctc tacatcgttc aaggagatgg aaccgcaaaa gatatcctgc tggaagaaag
    11521 tgctcaacac tatgatgccg ttgcgactct aacaggagtc gatgaggaaa atctgattac
    11581 atctatgttc cttgacaggg taggtgtaca gaaaaatatt actaaggtca atcgtaccag
    11641 tctcctcgag attatcaatg cgcctgattt ttcaagtatc atcacaccta aaagcatcgc
    11701 tgtagatacg attatgcact ttattcgtgg tcgagttaat gcccagtatt cagaccttca
    11761 agccatgcac catctagcca atggccaaat cgaaaccctg caattccata tcaaggaagc
    11821 caataaaatg actgccaaac ctctttctca actgaaattg aaaaaagggg ttcttattgc
    11881 agccattatt cgaaagggca agactatttt cccaactggg gaggatatgt tggaagttgg
    11941 agacaagctc ctagtaacaa ccttgttgcc aaacatcacc aagatttatg acttgattgc
    12001 gaggtaagaa atgaataaaa gtatgattcg ttacctcctt tcaaagttac ttttgattga
    12061 agctgttctc ctcttggttc ctgtggctat cgctgtctat taccgtgaat cgagccaagt
    12121 ctttacagcc ctcttttcga ccatagggat tctcgtattg ttaggcggtt cagggagttt
    12181 acagaagcca aaaaatcaac ggatttatgc caaggaggga gtcttgatcg ttgccctctg
    12241 ttggatcctt tggtctttct ttggtggtct cccctttgtc ttttctgggc aaatccctag
    12301 ccttattgat gccttttttg aaattagttc tgggtttaca actactggag caagtatttt
    12361 gaacgacatt tcggttctca gccgttccct cctcttctgg cgaagtttta cccacttgat
    12421 tggagggatg ggagtgcttg tttttgcact tgctattatg gacaatgcca aaaatagtca
    12481 cctagaagta atgaaggctg aggttcctgg tcctgtcttt ggtaaggttg tatccaaatt
    12541 aaaaaacact gcccagatcc tctatctcct ttacatagcc ctcttttccc tctttgtcat
    12601 catctattat ctagccggta tgcctctcta cgatagtttt gtcattgcta tggggacagc
    12661 gggaactgga ggatttaccg tctataacga tggaattgcc cactatggta gctcactgat
    12721 tacctatctg gttagtatag gagttttggt ttttggggta aatttcaatc tctactacta
    12781 ccttatgctc cgtcgggtta aggccttctt tggagatgaa gagcttcggg cttacttggt
    12841 catcgtgctg gtttctacag gcttgattag cctcaatact ctctacctct acccagggtt
    12901 ttcaaagagc tttgaaatga ccttcttcca ggtttccaac atcattacaa caactggttt
    12961 tggatatgga gatattacca actggcccct cttctcccag tttatcctcc ttttcctcat
    13021 ggcaatcggt ggctctgctg gatcaactgc aggtggactc aagattattc gaggcctcat
    13081 cctttcaaaa attgccaaaa accaaatttt gtcaatccta tcgccccacc gtgttttgac
    13141 cctccatgtt aataaaatgg tgattgacaa agatacccag cataagattc tcaagtactt
    13201 tgtcatctat gctatgattt tgctagcgct tatctttatt gtcagcctag atagcaatga
    13261 ttttctggtc gtgaccagcg ctgtctttag ctgtttcaat aatatcgggc ctattctagg
    13321 aaccacttct agtttctcaa tctttagtcc catctcaaaa attctcctct cctttgcaat
    13381 gattgcaggg cgcttggaga tttacccaat cctccttctc tttatgaaaa gaacttggtc
    13441 taagagataa aatacaaaat ccctcttttt acaattgtga aagagggatt tttatattaa
    13501 aaagggggaa cgtttttaaa tccaagcctg tctgtaagca aagttcttac tcaaataaaa
    13561 gatggaaaaa cttactacac agcttggtaa tgctccaata caaattgtaa ggctttactt
    13621 ggattctttg ttaaatgaat atattccatt cctctggtag caactatctg aatacctaat
    13681 ctttctgcat ccaccttcat ctctctataa taactccgaa cttgtggagc cagaataatt
    13741 aaatcataaa cacccataat atcataatga gctccgtacg ctcctgaatt cgcaatcact
    13801 ctaacttctg ctaattgagc gccctcgtta attgcattgg ctaattacgc acttgttcca
    13861 gaccctgcac aaagaaccag tactttcaac tccttacttt ttattacatc tatctcacca
    13921 ggcattattt cacttgtatc ctcctctaaa ataatatcat ttgagcttgc aatatctaca
    13981 acctgtagta gatatctcgt atttagacaa tatgaaaaca agacgacttc catataggaa
    14041 accgccttct ctctatgttg agtgatttat attaaaataa cttttcttct agctgcattt
    14101 tattattata aaaacattca tcataaccct gaacttaaat aacgattttt attcaagata
    14161 catactccta gaataaactt tatatgaaat tctcattttt gtttttacaa ttctccttag
    14221 ttaaatcttg tttaatatat gttttacata tagtatttag cgccacatag tactgaactc
    14281 tctccaaaaa cggttattcc tctttgaata gggcgttatc acaagaaaag catctccacg
    14341 tttcaacttc atatggctca aaaacaatca attgatgcta aaacctgtac ctagatgttt
    14401 cggttcataa aaccatgaaa ctgtaaaagt gaatgaaatt gatagcgata gtcaaatcaa
    14461 gaggcatcat aactctaaaa agtcacaata tataagttca tcctcggaaa aatatcattc
    14521 taattgttga aatgcctaca tgaaaagaaa cgtcaaatgc tcatgaaaca acgaatacag
    14581 gtatcaaaac tatgacaaaa caagtcccta aatttactaa agacactgct caactttaca
    14641 cctgtaagtg gttgttgtat aataaagtta caaagatgta cgaccacact gttgtaaatc
    14701 atagtgttcg cgaatatatt actgatagca tttctacaaa tacaattgaa gagactggat
    14761 agactttaaa cgaaatatgt cagtgttttg gcattcctag ccttcatatc atttaaagaa
    14821 ttctatagac aaaatttttc ccaatacaga cactcgtaac aactgcttca tttttctacc
    14881 aacatattta ggaacaggat aagatacaag aggattaatc catagatcag ttctatacca
    14941 atctaagaca aataagctaa aaaaacgatt gataataagc aaatagattc caaattttct
    15001 ctatctgctc attttaataa acaatactag tgtaactatc cttccagtca gaaacttgtc
    15061 aaatcacacc gaaaactctt ctaaaattta tctcgttagg caatcaagca aaaactcgac
    15121 gatagtacaa acattatcat acagaattga ctgcctaaat tatatacttt agtaaggttt
    15181 tcggataaga aaaaaggttc attttacatt tctaaacatt cttttctaag atgaaaaaca
    15241 gaatttttcg attgtgattt aaagcaacaa gaagattttc agtatcatcc tatagatacg
    15301 agctaatt
//
LOCUS       DAVLVF010000036        14157 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVLVF010000036 DAVLVF010000000
VERSION     DAVLVF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14157
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.036"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(166..780)
                     /locus_tag="VYN66_001161"
     CDS             complement(166..780)
                     /locus_tag="VYN66_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV1495190.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1595..1864)
                     /gene="rpsO"
                     /locus_tag="VYN66_001162"
     CDS             complement(1595..1864)
                     /gene="rpsO"
                     /locus_tag="VYN66_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV1495191.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(2005..2634)
                     /locus_tag="VYN66_001163"
     CDS             complement(2005..2634)
                     /locus_tag="VYN66_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1495192.1"
                     /translation="MKLAVIAANGQAGKAIVEEVVKRGHEVTAIVRSENKSQSESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            2872..3072
                     /locus_tag="VYN66_001164"
     CDS             2872..3072
                     /locus_tag="VYN66_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495193.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            3503..3826
                     /locus_tag="VYN66_001165"
     CDS             3503..3826
                     /locus_tag="VYN66_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV1495194.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDPLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            3884..5827
                     /gene="thrS"
                     /locus_tag="VYN66_001166"
     CDS             3884..5827
                     /gene="thrS"
                     /locus_tag="VYN66_001166"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV1495195.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFEKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5876..6850)
                     /locus_tag="VYN66_001167"
     CDS             complement(5876..6850)
                     /locus_tag="VYN66_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1495196.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6843..7520)
                     /locus_tag="VYN66_001168"
     CDS             complement(6843..7520)
                     /locus_tag="VYN66_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1495197.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7676..8749
                     /locus_tag="VYN66_001169"
     CDS             7676..8749
                     /locus_tag="VYN66_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV1495198.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDKLAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(8840..9142)
                     /locus_tag="VYN66_001170"
     CDS             complement(8840..9142)
                     /locus_tag="VYN66_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495199.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9210..9647)
                     /locus_tag="VYN66_001171"
     CDS             complement(9210..9647)
                     /locus_tag="VYN66_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV1495200.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKKSKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTDIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9723..10565)
                     /locus_tag="VYN66_001172"
     CDS             complement(9723..10565)
                     /locus_tag="VYN66_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1495201.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10677..11327
                     /locus_tag="VYN66_001173"
     CDS             10677..11327
                     /locus_tag="VYN66_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV1495202.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEILE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRMHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            11537..12172
                     /locus_tag="VYN66_001174"
     CDS             11537..12172
                     /locus_tag="VYN66_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEV1495203.1"
                     /translation="MAIASGYLNSRLDKFVDWGPWTALVPFGLISVTNVGISMLSTRF
                     TGKLSKWENYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWKASQEGRPN
                     QMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANALNALK
                     LTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVR"
     gene            12303..12740
                     /locus_tag="VYN66_001175"
     CDS             12303..12740
                     /locus_tag="VYN66_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495204.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(12835..13134)
                     /locus_tag="VYN66_001176"
     CDS             complement(12835..13134)
                     /locus_tag="VYN66_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495205.1"
                     /translation="MNRNVLNFLRTESAERVSLYIDKANRLEGDVTLLAPSSQDLEDI
                     KNAMFSNPNLELKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(13542..13904)
                     /locus_tag="VYN66_001177"
     CDS             complement(13542..13904)
                     /locus_tag="VYN66_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495206.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEKDVLILLSELQNFFLDVFQAKRIHRA
                     FCIRLCLRAQFLLSNNDS"
BASE COUNT         4347 a         3091 c         2460 g         4259 t
ORIGIN      
        1 ttcttatttc attttactat atttataaga atctcctaat caaatcaaaa gccactctac
       61 aatagagtaa ctttaagatt gtccatttat actcaatgaa aaagccccct gaaaatcaga
      121 gagctgattt gagctcgggc taaaatccta gtgaaacaaa aaaatctaca cgatcagaaa
      181 agtctctatc gtgccatttt catacatgat gtatagtcca agtagaatga atactaaggg
      241 cacaatgatt cgctcgtatt tttcaattgt ctcgaatatt aacggaatag aggataacac
      301 ccgactaatc tcgcaaaaga taattatgcc gattacaaac acaagcaagg ccacgagggt
      361 ctgtgaccaa tctaacgaag caaaataagg tatatagata cctaaattat ctccgccaga
      421 cgcaattgtc agcaatgtaa ctgtccaaaa cagttgattt gccttgcttt gttctaatct
      481 ttcaataatt tcttcctctt cttcttccgc atcttctcca acaattgcaa agcgaatccc
      541 taaatagata gggattaaac caagcaatcc aaccatccat tcttcaggca cgaaattaac
      601 gacataagca gcaactaaac tcgcccctac aagtaagcct gtgcctagat attgccccgc
      661 ataaatatgc catttctgtt tattctgtga tagctgtgca aataaaataa ttaaaataat
      721 taaataatcg atactggtgg aaatataaac accaatagca gatatgattg tctgtcccat
      781 aaaaaaaacc tcctgtatta gtcagtaata aatcagcaga ttttaggaga gcacagacac
      841 attaatttag ctatcgctag tgggtaatgt cgaacgtagg aaagcatttt actcctcctc
      901 aaaacgtagt tacaaagtaa tttctaactg gaattcagtg actacttcca tgtaaagtat
      961 aacatactat actttacttt gtaaagagtg ggctcttcta gggggcttgc atacagctac
     1021 tcggcttgaa aagccgagta actgcgcacc tttatggtgt aattagctga taccatttga
     1081 ggtttttgta gtctccaaaa ttgttaccga taaaacctca ataaaaaagc cctctgaagt
     1141 cagagagctg attttagccc gggctaaaat cctagtgaaa aagatgaaac tccttgtgct
     1201 catcgaacac tgtgtcattt ccctattttc atacggattt ttgacgccct tagcatcatg
     1261 attcttgcta gataaatcgt ttatagacta ggggacgagg tgaagatcgt acgaaacttt
     1321 cagtgaaaca aaaaaatctc cccgaaggga gaaaagctag tttattttac cttacagtgc
     1381 taggaaccgt aaccgaagat tatacttgag tatagcgagg taaggtgaca acgcaacaaa
     1441 gctacccaat ttagagtagc tttattatca agatatgctc aattgaacac ggcctaagca
     1501 cttggcaaaa aagataaaat ctcctagaaa ctgaagtttc ttcgtcaatt ttcctatttt
     1561 tgctttgtgt ttttgacggc ctttgtatct tgaattagcg acgaagtcct agagagttga
     1621 tcaactcacg gtaacggtta acgtcgtttt tacgcaagta tgcaagcaag ttacgacggc
     1681 gaccgatttt tttcatcaat ccacggtaag tagcgtggtc ttttttgtgt tgtttgatgt
     1741 gttcgttaag gtggttgatt tcccaagtaa ggacagcaac ttgaacctct actgaacctg
     1801 tatcaccttc gtgacgtgca tattgtgcaa tgatttcatt ttttttctct tttgagattg
     1861 ccatgatgtt tctccttttt atttggcttc atccgagtga caggttggca tgcctgtaac
     1921 caagaagaag ttatttgtct ttacgacaat ccctatttta ccaagattta aattttctgt
     1981 caaacattaa tactctcata gttattattt ttcaagtaaa gagatacgtt cttttatatg
     2041 atgacctttt tcaagttcat ctaccaatcc aatagcataa tctgcataac taatctggct
     2101 tattcctttt tcattagtag taaaaatttc tcctgctaga atgtagtttc ctgttttttc
     2161 accatcggga ataaaatcta cagcaggact gacaaaagtc cagttgagat tattttttgt
     2221 acgaagaaga tccaattcat ccgcttgagc ttttgctagt gacttaaatt cttcaggaaa
     2281 atcagacgta tctaaaagtc gtgtcgtttt tgtttcatct atataaagac ttcctgctcc
     2341 ccctacaact agaaaacgtg tctgtgttcc agcaagaagc tgattaaata gttcgattga
     2401 tttgctgtgg agcggtagcg tatctggtgt ataagcacca aacgctgaaa taacagcatc
     2461 aaatccagta agatcatctt ttgtcaactc aaataaatct tttttaataa tagactcaga
     2521 ttgacttttg ttttcagaac gaacaatagc cgttacttca tgtcctcgtt tgactacttc
     2581 ttcaacaatt gctttccccg cttgtccatt tgctgcaata actgctagtt tcatttttta
     2641 tacctctctt attgtaatta ttttagttac aacaattgtg acactcttaa taatcaatgt
     2701 caatagtttt gcttaattat tatcaaaata tttctaccaa gaaaactaac catgattcta
     2761 gtgaaaaaaa aatcttcttt gccaacaaat ttactttctt gttttaaaca tgctataata
     2821 atcatagcaa gagatctaag ttgtctgttt ttttaaaacg aggtgattat catgcgtaga
     2881 ttctattccc atctccccta ctatctggtc atattattct tttattggcc actttatgag
     2941 ttgttcttac tagttgtttc tgaccccctt acactcaagg aactctatat aaacaatctt
     3001 ctcttcttta cacctctggt aatcttgatt gtatcgttac tctatagcta ccgtttccgt
     3061 ttctcacttt gatggttagt tggtaacgga ctgctctttt actttactat cataaccttt
     3121 ggtgagttta tactaattta cttgctaatc tatgaaacag ttgctctggt gggcatggat
     3181 tctggtatta gcatcaagca tattctacaa aaaatgaaaa acaaaaaact ttcacaaaat
     3241 ccttgaaaaa tctcacaatc atgctataat aatccataga gacaagtcac ttagtccctt
     3301 tctactagag agtgcgtggt tgctggaaac gcataggaag tctaaactga tactactctt
     3361 gagtttttta tgaaaacata aaacggtggc cacgttagag ccaatcagag gtgtccctct
     3421 cttttgaggt acataaatga aggtggaacc acgttgcgac gtcctttcga ggatgtcgca
     3481 tttttttatt aggatactaa ttatggagtt gcaagaatta gtggagcgca gttgggcaat
     3541 ccgacaagct tatcacgaac tggaagttaa gcatcatgat tccaagtgga cggtagaaga
     3601 agaccccttg gctttatcta atgatattgg aaatttccaa cgactggtga tgacaaagca
     3661 aggacgctac tatgatgaaa caccctacac actggaacaa aaactttcag aaaatatctg
     3721 gtggctatta gaactttctc aacgtttgga tatagacatt ctgacggaaa tggaaaactt
     3781 cctctctgat aaagaaaagc aattgaacgt taggacttgg aagtagtctg atgataaaaa
     3841 atcaatgctt agaaactatg aaataataaa aaaggagaac atcatgatta acattacttt
     3901 cccagatggc gctgttcgtg aattcgaatc tggcgtaaca acttttgaaa ttgcccaatc
     3961 tatcagcaat tccctagcta aaaaagcctt ggctggtaaa ttcaacggca aactcatcga
     4021 cactactcgc gctatcactg aagatggaag catcgaaatt gtgacacctg atcacgaaga
     4081 tgcccttcca atcttgcgcc actcagcagc tcacttgttc gcccaagcag ctcgtcgtct
     4141 tttcccagac attcacttgg gagttggtcc agccatcgaa gatggtttct actacgatac
     4201 tgacaacaca gctggtcaaa tctctaacga agaccttcct cgtatcgaag aagaaatgca
     4261 aaaaatcgtc aaagaaaact tcccatctat tcgtgaagaa gtgactaaag acgaggcacg
     4321 tgaaatcttc aaaaatgacc cttacaagtt ggaattgatt gaagaacact cagaagacga
     4381 aggcggtttg actatctatc gtcagggtga atatgtagac ctctgccgtg gacctcacgt
     4441 tccatcaaca ggtcgtatcc aaatcttcca ccttctccat gtagctggtg cgtactggcg
     4501 tggaaacagc gacaacgcta tgatgcaacg tatctacggt acagcttggt ttgaaaagaa
     4561 agacttgaaa aactaccttc aaatgcgtga agaagctaag gaacgtgacc accgtaaact
     4621 tggtaaagag cttgacctct ttatgatttc acaagaagtg ggacaaggtt tgccattctg
     4681 gttgccaaat ggtgcgacta tccgtcgtga attggaacgc tacatcgtaa acaaagagtt
     4741 ggcttctggc taccaacacg tctacactcc accacttgct tctgttgagc tttacaagac
     4801 ttctggccac tgggatcatt accaagaaga catgttccca accatggaca tgggtgacgg
     4861 ggaagaattt gtccttcgtc caatgaactg tccgcaccac atccaagttt tcaaacacca
     4921 tgttcactct taccgtgaat tgccaatccg tatcgctgaa atcggtatga tgcaccgtta
     4981 cgaaaaatct ggtgccctca ctggccttca acgtgtacgt gaaatgtcac tcaacgacgg
     5041 tcacctattc gttactccag aacaaatcca agaagaattc caacgtgccc ttcagttgat
     5101 tatcgatgtt tatgaagact tcaacttgac tgactaccgc ttccgcctct ctcttcgtga
     5161 ccctcaagat actcataagt actttgataa cgatgagatg tgggaaaatg cccaaaccat
     5221 gcttcgtgca gctcttgatg aaatgggcgt ggactacttt gaagccgaag gtgaagcggc
     5281 cttctacgga ccaaaattgg atatccagat taaaactgcc cttggaaaag aagaaaccct
     5341 ttctactatc caacttgatt tcttgttgcc agaacgcttc gacctcaaat acatcggagc
     5401 tgatggcgaa gatcaccgtc cagtcatgat ccaccgtggg gttatctcaa ctatggaacg
     5461 cttcacagct atcttgattg agaactacaa gggtgccttc ccaacatggc tggcaccaca
     5521 ccaagtaacc cttatcccag tatctaacga aaaacacgtg gactacgctt gggaagtagc
     5581 caagaaactc cgtgaccgcg gtgtccgtgc agacgtagat gagcgcaatg aaaaaatgca
     5641 gttcaagatc cgtgcttcac aaaccagcaa gattccttac caattaattg ttggagacaa
     5701 agaaatggaa gacgaaacag tcaacgttcg tcgctacggc caaaaagaaa cacaaactgt
     5761 ctcagttgat aattttgttc aagctatcct agctgatatc gccaacaaat cacgcgttga
     5821 gaaataagag tctagcataa aagcctccaa tctggaggct ttttctcatc tatttttact
     5881 caaggactaa gttcacttga gcaaactgaa tccgcactgt cgttcctttt ccgacctcag
     5941 actcgatacg aatctggtgc cccagttctt cagaaatttt cttagataga taaaggccaa
     6001 gtccagagga ctgctgggtc aaacggccat tgtatcctga aaagccacgt tcaaatactc
     6061 ggaggacatc actgtttttt atcccgattc ccgtatcttt gatacaaagc tcttggtcat
     6121 ccatataaat ctccagacca ccttccttgg tgtacttgag actgtttgag atgatttgct
     6181 caataaccac tagcagccac tttttatccg tcacgatttc tttatcaagg tcatgtagat
     6241 tgacatttaa gcctttttga ataaagaaaa gagcatattt acgaattatt tccttgacca
     6301 agtcctcaat ttgaacctgc tttaagacca aatcatcatg gaaactttct aaacgcaggt
     6361 actgtaaaac taggttggta taagagtcga ttttgaaaat ttcctgttct agctgctgct
     6421 tcagttggcg gtcgaccact tctgcaacta agagttgact ggctgcaatg ggggtcttta
     6481 tctgatggac ccacaaggta tagtaatcca gcaaatccgt cagttttctt tctgcttttg
     6541 acctctgctg atagagttcc atctcacgcg cttctaattt ttctgctaaa gctatttcca
     6601 aaggagactt ggcttccctc tctccataga gaagttcctg gcgatagacc tgcatttcca
     6661 ccaatatgtc ccaagtgaaa aataagatgg ttacaaagca acacaagaag aaaaagtaga
     6721 ggaagtaaat tcctagactg gcaaataaaa actgaaagag taagacaaga aatgccaaag
     6781 aaagcagata gataaaaaga cgactacggg agcgcagata ggctagaaaa aattgtttcc
     6841 aatcaagcat gcttcaatcc gtaccctatt cctttcttgg tctcgataaa tcctaccaat
     6901 ccctgctcct ccaacttttt acgcaaacga gccacattga cagagagggt attatcatca
     6961 atgaaaaagt cactgttcca aagttcccgc atcaggtcgt cacgtgctac gatgttgcct
     7021 gcatgctcaa ataacacgcg taaaatctgg aattcattct tggtcaaatt caagacttgc
     7081 ccttgataat gtaaatccat ggatttggta ttgaggataa caccagcata ttccagcaaa
     7141 ctctcatcac gcccaaactc ataggaacga cgcaacaagc cctgaacctt agctaaaaga
     7201 acctgctggt caaaaggctt ggtcacaaag tcatccgccc ccatattgat tgccatgaca
     7261 atatccatag cctggtctct cgaagaaaga aacatgatag gtaccttgga aatcttgcgg
     7321 atttcctgac accagtgata accattaaac aagggcaaac caatatccat gaggaccaga
     7381 tgaggttccg actgaacaaa tagactcaaa acttccataa agtcttctac caggaccact
     7441 tcaaatcccc attcagagag cattttccca atctgttgac gaatgacctg atcatcttct
     7501 attaataaaa tcttgtgcat gcgcttctcc ttttccatta ttataacaga tttttccatg
     7561 ctagatggtc tgaaactgaa tttgaaatag cctgttttta gccagtgcaa acaggctatg
     7621 ctactagcta atttgaggga aatttgctaa gataaataaa aagaaaggag ctcttatggc
     7681 caatattttt gactatctga aagatgtcgc atatgattct tattacgacc ttcccttgaa
     7741 tgagttagac attctaacct taatagaaat cacctacctc tcctttgata atctggtctc
     7801 cacacttcct caacgtcttt tagatctagc acctcaggtt ccaagagagc ccaccatgct
     7861 tactagcaaa aatcgccttc aattattaga taaattggct caacacaagc gcttcaaaaa
     7921 ttgcaaactc tcccatttta tcaacgacat cgaccctgaa ctgcaaaagc aatttgcggc
     7981 tatgacttat cgtgtcagcc tcgataccta tctgattgtc tttcgtggga cagatgacag
     8041 tatcattggc tggaaggaag atttccacct gacctatatg aaggaaattc ctgctcaaaa
     8101 gcacgccctt cgctatttaa agaacttttt tgcccatcat cctaagcaaa aggttattct
     8161 agctgggcat tccaagggag gaaatctcgc tatctatgct gctagccaaa ttgagcaaag
     8221 tttgcaaaat cagatcacag cagtttatac atttgatgca cctggtctcc atcaagaatt
     8281 gacacagact gcgggttatc aaaggataat ggatagaagc aagatattca ttccacaagg
     8341 ttccattatc ggtatgatgc tggaaattcc tgctcaccaa atcatcgttc agagtactgc
     8401 cctgggtggc atcgcccagc acgatacctt tagttggcag attgaggaca agcacttcgt
     8461 ccaactggat aagaccaaca gtgatagcca gcaagtagac acaaccttta aagaatgggt
     8521 ggccacagtc cctgacgaag aacttcagct ctacttcgac ctcttctttg gcactattct
     8581 tgaggctggt attagctcta tcaatgactt ggcttcctta aaggcgcttg aatacattca
     8641 tcatctcttt gtccaagctc aatccctcac tccagaagaa agagaaacct tgggtcgcct
     8701 tacccagtta ttgattgata ctcgttacca ggcatggaaa aatagataat actcttgaaa
     8761 attaaatgta tacaaaacaa aagacctaga atacatactt tcatgtgcat tctaagtctt
     8821 tttaaataga atctaatagt caataaaaat caaagagcat tgagagataa tggggcttgg
     8881 aacgtccctc tcgcttcaac aaaatgaccc cattatagat taaaaagatg ccacttagaa
     8941 aaagcaaaaa aggaagtaag acaaaggcaa atacataaaa agctaactga acattctcgt
     9001 atccattttt ataaaaaagg taggatagat aaaaataact tgaaatgagg gataataaaa
     9061 ataatactgg attccacaaa cttctattat ccttccaaaa tgacactata aaggctaata
     9121 caattcctat aacgagatac atttcttact cctttaatag ctacatttta tcataattat
     9181 ccaaagaaaa aagagggcat ttatccctct taatccttca tctgactctc tgcatcggcc
     9241 acgacttttt ctagactggt ttgaccaagt tctgcctcca tagtcaactg aattctctcc
     9301 aatttttgat ccaaaacatc atgaatatga gctcctacag ggcaatttgg attcggattg
     9361 tcatggaaac tgaagagttg acctgtctta ccaagacatt cgaccgcctg ataaacatct
     9421 aaaagactaa tatccttaag gtccttgaca atatctgttc cgcccgttcc acgcgctact
     9481 gaaatcagct ctgccttctt caactgggac aagatctttc tgataatgac aggattgacc
     9541 ccgacactag cagccagaaa atcactggtc accttgcttt tcttcccctc gagggcaatg
     9601 attatcagca tatgagtcgc aatggtaaat ctacttggaa tttgcatcct cttctccttt
     9661 ttacgaggct accctgcctc tactcttctt tttctattat tatacccttt ttagttgtaa
     9721 tgtcaatcgt taccactttt caaccagtcg tctaactccc gatcgcaacc ctctttctga
     9781 gccaattctc tcaaaaattc ctgatgatga gtatggtgga tcccattgac cagactttca
     9841 tagtaaacct caaaataggg aagtctcagg tctttagcca gctgcaattc agctgccaca
     9901 tcgtaatcta cccgtcggaa gtccatatct accaggccct tgtcatcaaa ctccaaaatc
     9961 atatactggg cccgcaagtc cttccgtagc tgagcgtcca aaaagaaagg ttggccaatc
    10021 gaacccggat tgacaatcaa ttgcccacca gtcccgtaac gaagcaactg ctggtgaatg
    10081 tgtccataaa cagcaatatc acagggagga tgagtcacca agcggtcaaa ttcctcttgt
    10141 ttgccagtat gaatcaactc tcgcccccag ttcttatcag gcagatgatg gctaattccc
    10201 actgtcaaat ccccaaactg acgatgaatt tgaagaggtt gattgtggag cacttcaatt
    10261 tcttctaggg aaatttcctc taaaacatac tggcactggc gcaagagata gcgttgacta
    10321 gggcgagtac tatccaattc cttacggaca ccatgccaaa gactgtcttc ccagtttccc
    10381 aaaactctag ccgtaatcgg tagttgatcc aacaagtcca aaatccttct acgccctgtc
    10441 cctggcatga gaatatctcc caaaagccag tattcatcca ctcctagctg ccgagcatct
    10501 gccaaaacag cctccaaggc ggtggtattt ccatgaatat ctgaaagaag agctattttc
    10561 gtcatatcca tctcctcgtt ttttctcttg caataagtat aacataaaaa gtcacagcta
    10621 gagaaatcta gctttttttg atatactaga taaagatatt agacaagagg aaacgaatga
    10681 ccccaaacaa agaagactat ctaaaatgta tttatgaaat tggcatagac ctgcataaga
    10741 ttaccaacaa ggaaattgcg gctcgcatgc aagtctctcc ccctgccgta actgaaatga
    10801 tcaaacgaat gaaaagtgaa aatctcatcc taaaggacaa ggaatgtggc tatctactga
    10861 ctgacctcgg tctcaaactg gtctctgagc tctatcgtaa gcaccgcttg attgaagttt
    10921 ttctagttca tcatttagac tatacaagtg accagattca cgaggaagct gagatcttgg
    10981 aacacactgt ctctgacctg ttcgtggaaa gactagataa actgctaggt ttccctaaaa
    11041 cctgccccca cggaggaact attcctgcca agggagaact cctcgttgaa atcaataacc
    11101 tcccactagc tgatatcaag gaagctggcg cctaccgcct gactcggatg cacgatagtt
    11161 ttgacattct ccattatctg gacaagcact cacttcacat cggtgaccag ctccaagtta
    11221 agcagtttga tggcttcagc aataccttca ctatcctcag taacgacgag gatttacaag
    11281 tgaatatgga cattgcaaaa caactctatg tcgagaaaat caactaattt ctcaagtccc
    11341 ctaccaaccc tgaaagtttt attttcaggg ttttatttct atcattttat tttactttca
    11401 tttggtggta gaatatttga aaaaaaatgg gagaatattt gaaaaaaaga aaggtagttt
    11461 aacctatgaa aaaatcacta aaaatttttg ctacatctaa atagtttgac ctcttcgggg
    11521 ttgctttggt cgttggattg cgattgcatc tggttacctc aactcacgcc tcgataaatt
    11581 tgtagactgg ggaccatgga cagctcttgt accctttgga ttgatttccg taaccaacgt
    11641 tgggatttcc atgttgtcca ctcgtttcac ggggaaatta agcaaatggg aaaattactt
    11701 tggtattgtt aataccattt tgtccggtgc tattgattat atccttggaa ataaggcggc
    11761 catcattacc tatcccgtca ccttcctcat ttataccttt gctataaaga aatggaaagc
    11821 ttcgcaagaa ggcagaccca accaaatgag ccaaaaacag gtaaaattgg cggccatcat
    11881 catttccatc atcgccttcc tctttgcctt tgtgaccaac tatatcggat atgggggcaa
    11941 gatgaatctc cttgcctacg taacaactat tgcctttgca ctgtccctca ttgccaatgc
    12001 tttgaatgca ttaaaactaa caactcagtg gggcttttgg ttgatttaca atttcgttca
    12061 gctgacaaaa gctggtattc aaggaaactt cgccaatatc ggtaaataca tcttttatat
    12121 cctcaatgca atcggagctt tatttgtctg gaatgatgaa gaagttagat aatagaagga
    12181 aactcaaata aagaatacta gccaacttaa gagaatctcc cctctgctat ttttatcatt
    12241 ttagttgtaa ctcccattct tttagagtag aatgaagtta gataaaagag ggaggtcttg
    12301 tcatgaaaaa actctataga atccatttta tagcaattgc agttattgat ttgctactat
    12361 ttgcattttt tatcactaga ctcgagacat cttttgagtg gctcttgctc tctggtttaa
    12421 ttttcttcct tgctcaagga ctcctgctat ttctcttggt cgtccgactc aaacatcaat
    12481 tcgctgagat ttatcctcaa atcaataaaa agattcgctt ctactattta ggggttctca
    12541 ccattgattt tctatttttt gttctcttag ccttcattag ttctcagcgt ttttcatctc
    12601 ttatgccaat catcactgct tgccattcta ctttttatta tatgacagct gactacctaa
    12661 gagaaaacta tccagacttt tacgacaaac acatctctaa cacgtatact attttatcga
    12721 tttcttcttt tttagtataa gacagctcgt tctccagaaa tattcattaa ctttcatttt
    12781 tatacttttc ctttcgattt tcaaaaacaa ggggagggat taacctccct tatattagac
    12841 ataactttta ggatcacaat tgtatgttcc tttcgaaata tccccaaaaa tcgtagcccc
    12901 tgttaaataa tgatttcttg tagaagcata tgctattttc ttcatcacat ccagtctcgc
    12961 tacttttagc tcgagattag gattggaaaa catagcattt ttaatatctt ccaaatcttg
    13021 agaactaggt gcgagtagag ttacatcgcc ctccaatcta ttagccttgt ctatataaag
    13081 actaactctt tcagcacttt ctgttcgcag aaaatttagt acatttctat tcattttttt
    13141 acctcgtatt tttctaatta ttgatttttt ctagaaacaa aatcaattca acggtgaagc
    13201 tctagctttc atcttcattc gtacctcctt ttgtgtatat ctagctttac ataaacctat
    13261 gatttagagt atagtttctc aattattctc agaaactatg gagatgattt aattatcata
    13321 caaatcaaag acctctcctt gctcatcttc accgtaactc tcgagttcct cataaaagta
    13381 ttccaaagca gtttgttctt ctggtgtcat aaagaaactc ctttctaaac aatattagta
    13441 aaaaatacgt tcttatttcc atcctaaata aagtagtgtt tttatcccgt aataggtttt
    13501 atttttgcgg agcattacac tccgcattaa tattgatagt attaagaatc attattactt
    13561 aataaaaatt gcgctctcaa acatagacgt atacaaaatg ccctatgtat acgtttcgct
    13621 tggaagacat ctaagaaaaa attttgtagt tcagacagta agattaatac atctttctct
    13681 aacataagtg atgtctggta ctcccaatta ttgctctgtt ttaacgatga aaaaggtata
    13741 gctgtctctt ttaattttat ccaatctata cgttcttttt taggaacgta ttctctaaca
    13801 gcttgactaa caatactgga atagcttact tcatagccta atttattgta aacatcatct
    13861 ttaagcttat ctaatatctg tctggattca gacgctaaac gcattctaac attctctttt
    13921 cccatattat cacccctttc tgacgggatt ctgttatccc gtaaattaag tatataacaa
    13981 tttagaaaaa aaatcaagaa ttaagttgaa aaaaattcca caatctaaaa atttatcatt
    14041 ttatgacaaa catactatcg ttaaatgctg atttaatcga aattttgagt tataaactcc
    14101 tcagtttcga actcaaaatt agccgtgcag gataggatac ccttcataaa cttatat
//
LOCUS       DAVLVF010000037        13549 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVLVF010000037 DAVLVF010000000
VERSION     DAVLVF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13549
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.037"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(209..1177)
                     /locus_tag="VYN66_001178"
     CDS             complement(209..1177)
                     /locus_tag="VYN66_001178"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1495207.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVAVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1322..2105)
                     /locus_tag="VYN66_001179"
                     /pseudo
     CDS             complement(1322..2105)
                     /locus_tag="VYN66_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            complement(2130..2627)
                     /locus_tag="VYN66_001180"
     CDS             complement(2130..2627)
                     /locus_tag="VYN66_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV1495208.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2700..4061)
                     /gene="radA"
                     /locus_tag="VYN66_001181"
     CDS             complement(2700..4061)
                     /gene="radA"
                     /locus_tag="VYN66_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV1495209.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINGAAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4075..4590)
                     /locus_tag="VYN66_001182"
     CDS             complement(4075..4590)
                     /locus_tag="VYN66_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1495210.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4592..5035)
                     /locus_tag="VYN66_001183"
     CDS             complement(4592..5035)
                     /locus_tag="VYN66_001183"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV1495211.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            5340..5489
                     /locus_tag="VYN66_001184"
     CDS             5340..5489
                     /locus_tag="VYN66_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495212.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            5631..5810
                     /locus_tag="VYN66_001185"
     CDS             5631..5810
                     /locus_tag="VYN66_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495213.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(6030..6395)
                     /locus_tag="VYN66_001186"
                     /pseudo
     CDS             complement(6030..6395)
                     /locus_tag="VYN66_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            6415..6582
                     /locus_tag="VYN66_001187"
     CDS             6415..6582
                     /locus_tag="VYN66_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEV1495214.1"
                     /translation="MANTPIKPGTDNQKPGRYVEVGPRGGKVTNGHTATIGKGDRLPP
                     TSAKGNGWKKV"
     gene            complement(6737..6940)
                     /locus_tag="VYN66_001188"
     CDS             complement(6737..6940)
                     /locus_tag="VYN66_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495215.1"
                     /translation="MRPKRYPYSGQKESTFVKADPELVEKLLRNTSFLECLQKKPINF
                     QIDSEEFKRLSYEAIHDTSQVTQ"
     gene            complement(6963..7154)
                     /locus_tag="VYN66_001189"
     CDS             complement(6963..7154)
                     /locus_tag="VYN66_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1495216.1"
                     /translation="MPDIANGRERVIAFLKEKGIKKATLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
     gene            7725..8093
                     /locus_tag="VYN66_001190"
     CDS             7725..8093
                     /locus_tag="VYN66_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1495217.1"
                     /translation="MFETFEKIKELAKKRGKALGQVEEDLGYGRNTLYKIKNSTPNAE
                     RIAEIANYFNVSTDYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            8093..8326
                     /locus_tag="VYN66_001191"
     CDS             8093..8326
                     /locus_tag="VYN66_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495218.1"
                     /translation="MTITINFTEKNSYITDYLNKHGIDTTTMDFDDFMALMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLTREDIA"
     gene            8326..8589
                     /locus_tag="VYN66_001192"
     CDS             8326..8589
                     /locus_tag="VYN66_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1495219.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            8602..8982
                     /locus_tag="VYN66_001193"
     CDS             8602..8982
                     /locus_tag="VYN66_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV1495220.1"
                     /translation="MTLAKLCEEYQVELCLFDGSNWHNSGFYNPDTNVLAIDHNLTPE
                     QQIQVALHELGHKDHTRSEHQNARLRCENEADRNMIHHLVKDALENLDDPTEFDYLKF
                     MSYYNLKTMTNEIMVKEEYLALVN"
     gene            8999..10069
                     /locus_tag="VYN66_001194"
     CDS             8999..10069
                     /locus_tag="VYN66_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEV1495221.1"
                     /translation="MEIDKVKADLKQVGKRVADLSQSITNEEQTKNAFIMPFFQALGY
                     DIFNPLEFVPEFTADVGIKKGEKVDYAIILDGEPQILIECKSITENLTKHDSQLFRYF
                     VTTKSKFGILTNGREYKFFTDLDEPNKMDTTPFLTIDVTDIKENQFTEIIKFHKENFD
                     IDNIVSSASELKYLNNLKAFLTENITTPSDSFLRYLTSEIYEGRVTQNILTTFSPIIV
                     KGFNQFITERVNEKLSAALNTSVETKVTTDIPKVEAEAEEIVEVTDEIITTPAELEVY
                     TVVKMLARDVVSPERVFYRDNRSYFNVLVDDNIKKWVLRYRSNSKKSTIEIRDKGIFP
                     VSTPLEVANYANEILEVIKKFS"
     gene            10124..11265
                     /locus_tag="VYN66_001195"
                     /pseudo
     CDS             10124..11265
                     /locus_tag="VYN66_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518706.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(11348..11441)
                     /gene="ffs"
                     /locus_tag="VYN66_001196"
     ncRNA           complement(11348..11441)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VYN66_001196"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(11474..11941)
                     /locus_tag="VYN66_001197"
     CDS             complement(11474..11941)
                     /locus_tag="VYN66_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV1495222.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(12142..13428)
                     /locus_tag="VYN66_001198"
     CDS             complement(12142..13428)
                     /locus_tag="VYN66_001198"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV1495223.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
BASE COUNT         4463 a         3040 c         2286 g         3760 t
ORIGIN      
        1 cctactagaa ttctccttag aaagcgtcga ttcaagtgct atcactgttc aaaaatgatg
       61 gtcgctgaaa cttctgatga cgtatagtca tatttcttct ctttttatta tatcacagtt
      121 ttaaatctag ctttactaga ttcaccgcta ctatccattt attcggaaaa aagacgaaaa
      181 aacctgagaa tcatctcagg cttggtcatt aaattttttt ctcaatatcg aaaagtggag
      241 aaagtggtcg tttttcatga atacgtacga tagcatcccc taggagatga gcgattgaaa
      301 tctgctcaat cttatcaatc aaacgctctt ctggcagata gatggtatcc aaaacaacca
      361 atttcttaat agctgatttt tggatattgt ccgtagcagg accagaaaga actgggtgcg
      421 tacagcttgc atagactgca acagcaccag cttccgcaag agcatctgcc gcatgacaaa
      481 tcgttccagc ggtatcaatc atatcatcaa tcaagataca agtcttgcct tcaaccttac
      541 cgatgatgtt cataacttca ctagtattca tcttatcaac gctacgacgt ttatcaataa
      601 tagcgataga tgttttcaaa aattctgcca acttacgagc acgagtcacc cctccatggt
      661 ccgggctgac aaccacatag tcagaaccaa ccataccacg ccgctcaaaa taatctgcaa
      721 ttagaggagc acccatcaaa tgatccacag gaatatcaaa gaatccttga atttgcgcag
      781 catgcaagtc gatggtcaat aaacgatcca ctccagctac ttcaagcata tttgcgacaa
      841 gttttgaagt gattggctca cgcgctctcg cctttctatc ctgacgtgca tacccatagt
      901 aaggcatgac aacattgaca gactctgcac tcgcacgctt caaagcatct accataatca
      961 aaatttcaag cagattgtca tttacaggcg aactagttga ttgtaagata aagacgtgtt
     1021 tcccacggat tgattcttca atgttgacct gaatctctcc atctgaaaat tggcgaacac
     1081 ttgatttccc caactctatc ccaatctcct gcgccacacg ttctgccaat tctttattag
     1141 aagaaagggc aaacagcttt aaatcagaaa aagacatgat ttcctccggt atatatgtat
     1201 cgcttgtgct tttcacaaga ttttccatct accattgtag cgctttttgc actatttttc
     1261 aatcaaaaat aaaagaaggg caccatattt gtacccttgc atcattcttt tgaaaaatat
     1321 tctaggtcat caactcattg tgtttctcaa caaagcaata agcatgataa taaccataga
     1381 gagcaatagc cgtaaccact ggaatcgcta aaggcaactc tgtttccaac tccacaaaag
     1441 gagagttaaa caataagacc ctgtttcttg ccaaccttct gtcctttata ggctctgtaa
     1501 agcaagtaaa cacctactac agctagacct gaaaaagtcc agtgagaggc aattcctgag
     1561 atgatacgct ctaaaattcg cgaaatagta aagtcaaagc cctctggcaa atccgtacga
     1621 atataaccaa tatcctcaat catttggaat cccaaaccgg aggcaattcc aagtaaaaac
     1681 aaaaatttta attttcgcac aggaatcaaa gccaaaacaa aaacaagtgg caataatttc
     1741 aagggttctt ctaccaaagg agccacaata gcactttcaa aggcatttaa aaatggacta
     1801 tctgggaaaa gaacccccag taaatcatgt atataagtat tagcaaaact agacaaccag
     1861 cctgaaagga acatccctcc caataaagac agaatcaaaa ccttctttgg caattcccat
     1921 tttttcccaa tacggaagag aaaataaaga gccggaatca tgtaaaagag agctagaaag
     1981 atagaaactc ccattagtcc atattccgca cctgacctcg aaccgtccgt atagtagatg
     2041 gtttcatact gtaaaccaat acatagcaat aaaataaaaa taaataaaat actgcttttc
     2101 ttcatacact ttctttctaa atgaagtatt tataattcta cgactgtcat acttcctgta
     2161 tcaacattgt aaatagcacc agagataatg acatcgtctg gtattagggg agactcgata
     2221 agcagttgca tatcctcgcg tacactctct tctatatctt ggaagggcaa gaagtcctgg
     2281 tctgacacat ctacacctaa ttcctctttt aaatactcct gaaaaggttc attttcaaag
     2341 gtctgagcac cacagtctgt atggtgcaat accacaatct ctcttgtccc catttgttgc
     2401 tgggaaataa ctagcgaacg aatcatgtct tcagtcactc gaccacctgc attccgcaag
     2461 atatgagcat ccccaagtgc caagcccaga gcttgcgcaa cgtgcaaacg tgagtccata
     2521 caggtcacaa tggctactct ggttttaggt ttaagtggca gatttaactg cccatgtagg
     2581 gcaacataag cctgattggc ttgcataaac tgttcaaaat acgacacgat tccctccttg
     2641 aaaatttgat agtcaaatat ttctcctatc ttatcatttt taagagaatt tgtcacggat
     2701 tatgcaaaga cctttttcaa gacttcctga atcgttgtca cgccaatgac ctgaatttcc
     2761 ttaggcagag tgattcctgt caaggaattc tgaggtacat aaatcttagt aaagcccagt
     2821 ttagcagctc cgttgatgcg ttgctcaata cgattcacgc gccgaatctc tcctgtcaag
     2881 cccagttctc cgacaaaaca ttcctgagga ttagttggct tgtctttgta gctcgaagca
     2941 atagcaactg caacagccaa gtcaatggca ggttcatcca atttaacacc accagcagat
     3001 ttgagatagg catcctgatt ttgcaagaga agccctgccc gtttttccaa aacagccata
     3061 atcaagctag cacggttaaa atcaagtcct gtcgtagtac gcttggcatt tccaaacatg
     3121 gtcggtgtta ccaaagcctg aacctccgcc aaaatcggac gcgtcccttc catggttaca
     3181 acgatggagg aaccagtcgc cccatccaaa cgctcttcta ggaaaacttg actcggattg
     3241 agtacctcaa ccaagccgcc cgactgcatc tcaaaaatcc caatctcatt agtggaacca
     3301 aaacgatttt tgaccgctct caaaatacga aaggtgtggt gacgctcccc ttcaaagtaa
     3361 agcaccgtat ccaccatatg ctccaacata cgaggcccag ccaaggttcc ttctttggtc
     3421 acatgaccta cgataaagat ggcaatgtta ttggtcttgg ccaactgcat gagttcagcg
     3481 gtcacttcac gtacctgaga aacagacccc tgcacccctg aaatctcagg agacatgatg
     3541 gtctggatgg aatcaataat gagaaagtct ggctggatac gctccacttc tgcacgaaca
     3601 ctctgcatat tggtctctgc atagagataa aactcactat caatatcacc taagcgctct
     3661 gcacgtagtt taatctgctg ggcagactcc tccccactga catagagaac tgtccccact
     3721 tgggacaact gggttgagac ttgtaggaga agagttgatt tcccaatccc aggatcccca
     3781 ccgatgagga cgagacttcc tggtaccact ccgcctccaa gcacacggtt gaattcctcc
     3841 atctccgtct tggttcgatt gacattgatg gaagtcacct cagctagttt catgggcttg
     3901 gttttctcac ctgtcaagga cacacgcgca ttcttgacct cggcaacctc aacctcttcc
     3961 acaaaagaag accaagaccc acagttgggg caacgtccca gatatttagg ggaattatac
     4021 ccacaatttt gacatacaaa tgtcgctttt ttctttgcga tgacaaacct ctttctatat
     4081 ctctaactca cactcaatca cttggcaaaa atcaatcttc tcatttggca caaactggcg
     4141 catgagcatt cgatgagcaa caactaccac agtctgatgt tctcgatact tagacataca
     4201 ttctagaaac cgagacttca tttccgtagc tgtctcatat tgaataggac tattaggaag
     4261 caactccccc ttgttttcta aaaacagtct tctagctgtt tcaaagtttt ctattcctgt
     4321 tttatagacc tgccattcat gtaataaagg ctctactctt aaaggaagac ccgtagcaca
     4381 gaccacatac gaagccgttt ctaaagctct tgtgactgca gaagatacga ttatttcagc
     4441 tgacgagagt aaaggatttt tgctcaattt ctggacttgc tgccgtccca tctcagacaa
     4501 gggtgccaaa tctatcccaa atcctatata agaacgctcc tctaactcac ggtaatctgg
     4561 ctccccatga cgtacaaaga taatcttcat tctagtgccc tgtcgatcca aatccaccag
     4621 ttcgaacgcc atcagctgca tctccatctg caattaagaa agtagcaaaa acagcctgga
     4681 caatacgttc cccaacttca agaacaacct cttgatctgt gatattcttc atctgcgcaa
     4741 aaatatgccc ttcatttcca ggatttccat aataatcccc atcaatgacc ccaactgagt
     4801 taattaaaac caagcccttc ttacgaggat ttgaagaacg atcatagagg tagagaacct
     4861 cagtcggctg catataagcc ttaacccctg tcggaaccaa gacaatctct cctggcgcaa
     4921 caactgtacg cacagcaacc tttaagtcgt aaccagccgc atgcgctgtc tcacgcttgg
     4981 gcaataaatt ttcatctgta aaactcgaaa ccaattcaaa accacgaatt ttcataattt
     5041 tctcttttct attatcattt attctagatt attctatctt atttattcgg aaaaagcacg
     5101 aaaaaaagag cacacaacaa ttataggcga tacgataatt tacactgttt cacaatacgt
     5161 tgaaatttag agctttaaag cgagggcaaa gttgattttt tacatcgttt tacagaggtt
     5221 tacgacactt ttgccccttt tttgcccctt tgtttttcaa aaaaacttca tcaaatcgct
     5281 tgactttcac ggtttaccgt gatataatat aatcaagata aggaaaggag gtgaggaagt
     5341 tgaacaaaga agattggctt aggttactgg aaaaggcgat agataatatc cctgaaacgg
     5401 taactgctat agcaagtctg gtgactgcaa taacggtcgc aaggcaaaac aaaaagcgta
     5461 aaccgaaatc ccgtcaaaga aaaaggtgaa cgctaagagg ttggggcgaa agcccctcac
     5521 acctctattt tatcaaatga aaagaggaaa agcaatggtt agtgcaatag ctatttttat
     5581 aattgcggtc aatgtatata tttatctaaa aaataaaaag gacaaataag atgagagaag
     5641 ttattcaaga attgttagac agttcgatgt ctacatctgc tatttcccaa ggtgctggag
     5701 ttccatggac tactgtttct gacctcagaa aaggaaaaac aagcatggac aaaatggcac
     5761 ttctcacagc agaaaaactt tatgaatttg ctacaactga taagcagtga tttcggtcac
     5821 tgtttttatt tttgacaaaa caccgttttt gacaataatc aaaaaataaa aaggagctat
     5881 tattaacaaa atggcgtttt tggcaataat accctgccat tcctcctccc tattcttcaa
     5941 gaaaacgctt ttttgaagaa tcagagcaaa taaaaaaccg caagcctgag cctgcggtga
     6001 aagaacaatt tagaaagttt cctttctatt tatttaactg taatcaagcc atctggctct
     6061 actgtgaact ctggcttgtc tgccagtgtt ccgtctggtt tgaggtagta ccagcctgtt
     6121 ccgtccgctg actggataaa ggcatttgat accatggcgc cttctttagc gtctaagtag
     6181 taccaagtgt ccttgtactt gacccagcct gtcttcatgg caccttctac atcaaaatag
     6241 taccacttgt cagcgatttt cttccagcct gtggccattt cgcctgattg gtcaaagtag
     6301 taccaattac cgtctgtgtg cttcttccag cggtctgaaa gcatatagcc tgagccatcg
     6361 aaataatacc agataccgtt gattttctca aacttataga aaggaggaga tagtatggca
     6421 aatactccaa taaaacctgg aacagataat cagaaacctg gtcgctatgt agaggtagga
     6481 cctcgtggcg gaaaagttac taatggtcat accgcaacta tcggaaaagg tgatcggctc
     6541 cctccgacat ctgctaaagg caacggttgg aaaaaagtct aatcttcgtt tgcgtacaat
     6601 cgttcaatgg ttgtacgctt tttccataca caaaagcaca ttcccaaaaa atcaatttga
     6661 atccatgctt cggcgtaatc tttcccattg ctggcatagt gagttatata atggtgaatc
     6721 attttattcc ttcctcctat tgagttactt gagaagtatc atgaatggct tcatagctaa
     6781 gacgcttaaa ttcttctgag tctatctgaa aattgatagg ctttttttgt aaacactcaa
     6841 gaaaactagt gtttcttaaa agtttttcaa ctaactcagg gtctgccttt acaaaggtgg
     6901 actctttttg tccactatac ggatatcgtt ttggtctcat tttcctactc ctcaaatctt
     6961 tcctactcaa tcccataatc ttcaataacc tgaagaatga aactgttcgc tcgtggacct
     7021 ttagtcgttc cacttagaat gtttgtcact tcctgtcgtt taaagccgta agcaaccgct
     7081 agagttgctt ttttaatgcc tttctctttc aagaaagcaa taactctttc gcgaccgttt
     7141 gcgatatctg gcatattttc tcctttcttt ttctttcttc tttttctgct ataatataag
     7201 cagaaaggag ttaaccttat gacttttaaa gaatatttac tcaaagcaag caaacgcgac
     7261 atctacgatg atggtaaaga ttttgagaaa tcaatttcag attctcacta atcgagcgtg
     7321 acttttttga tatggcaatc tcagaaattt accaccagcg ctgactacct taatgacttt
     7381 ttcaaggtgg tctttttctt gttctaaatt tttaagaata cttaccatca gcacgttcct
     7441 cctctttact tatttgtaaa taagagacaa ctaaaaatgt aactattttt ctgcattata
     7501 cttgacaact tacaccaaat cggctaaaat taaagcataa taaaaacacc aataaatcta
     7561 taaataccgt tcgccaaaac atttttataa tttatttctt agttgttttt ttagttgttt
     7621 atcacttaca aaaaacattt tacacctttt gggataatta gtcaaccttt ttacaccaaa
     7681 tttgttaaat ttttttgtga tgtcttagaa aggttgattt aacaatgttt gagacatttg
     7741 aaaaaataaa agaattggca aaaaagcgtg gaaaagctct tggacaagta gaagaagact
     7801 taggttatgg tagaaataca ctctataaga taaaaaattc tacgccaaac gctgaacgta
     7861 tcgcagaaat tgctaattat ttcaacgtat ccaccgacta cctgctcgga cgaacagata
     7921 accctgctat cgctgggagt gatgaatttg cccaagtaaa cggacaaatc atagacttac
     7981 gcaaagcagc agccaacacc atgttatttg acgggaaacc actaaatgaa gatgatatcg
     8041 acttcattac atccgtccta tccgcccact tcaaaagcaa aggagaacgc taatgactat
     8101 cactatcaac ttcacagaaa aaaattccta cattacggac tacctaaaca aacacggtat
     8161 cgacacaacg accatggatt ttgacgactt catggcactc atggaagata tcgaagacgc
     8221 acgagcagcc gaccaagcct atatggagta tttagccgac ccagctactt ataccatgga
     8281 tgaggtcttg gatgaactag gactaactcg agaggatatt gcttaatgta tcggctagat
     8341 attgataaaa aagctctcaa gcaacttaaa aaactagata ccccaaccag aaaacaaatc
     8401 ctatcctggc ttgctaaaaa cattgaaaac acgaccaatc cacgacaaca cggaaaagca
     8461 ctaaaagcca accttgcagg ttactggcga tacagagtag agaattaccg catcatctgt
     8521 gatatccaag acgataaact agtcgtccta gccgtggaaa tcgcccaccg cagagatgtc
     8581 tataaataac gaaggagaac tatgacactc gctaaactct gcgaagaata tcaagtagaa
     8641 ctttgtctct tcgacggttc aaactggcac aatagcggtt tctacaatcc agacacaaac
     8701 gtgctcgcta ttgaccacaa cttgactcct gaacaacaaa tccaagtcgc cctacacgaa
     8761 cttggacaca aagaccacac acgctcagag caccagaacg cccgtctacg ctgtgaaaac
     8821 gaagctgata ggaatatgat ccatcatctc gtaaaagacg ctctagaaaa cttagacgac
     8881 cccacagagt ttgattacct caaattcatg tcttactaca atctaaaaac catgacaaat
     8941 gaaatcatgg ttaaagaaga gtatttagca ttagtaaatt aaaaaaagga gtatcaatat
     9001 ggaaattgat aaagtaaaag ccgatttaaa acaagtcggc aaacgtgtag cagacctcag
     9061 ccaaagcatt acgaatgaag aacaaacaaa gaatgccttc attatgccgt tcttccaagc
     9121 gcttggatat gatatcttca atccacttga atttgtcccg gaatttactg ccgacgtggg
     9181 cattaaaaaa ggcgaaaaag tcgactatgc gatcattttg gatggcgagc ctcaaatcct
     9241 aattgaatgt aaatcaatta cagaaaacct tactaagcac gactctcaac tattcagata
     9301 tttcgtaact actaaatcaa aattcggtat cttgacaaat ggtagagaat ataaattctt
     9361 tactgattta gatgagccaa ataaaatgga cacaactcca ttcttaacaa tcgatgtgac
     9421 tgatatcaaa gaaaatcaat ttactgaaat catcaaattc cacaaagaga attttgatat
     9481 tgataatatt gtttcatcag cttctgaatt gaaatacctc aacaatctta aagcgttcct
     9541 tactgaaaat atcactacac cgtcagatag cttcctcaga tatctaactt ctgaaatcta
     9601 tgaagggcgt gttactcaaa atatcttaac gacattttct cccattattg tgaaagggtt
     9661 taatcaattc atcacagagc gagttaatga aaaattaagt gctgcactta atacaagcgt
     9721 tgaaaccaaa gtaacaaccg atattccaaa agttgaagct gaagctgaag agattgtcga
     9781 agtaactgac gaaattatca caactcctgc tgaattagaa gtttatactg ttgttaaaat
     9841 gcttgctagg gatgtagttt ctccagaacg tgtattttac agagataacc gtagctattt
     9901 caatgttttg gttgacgata acatcaaaaa atgggtatta cgttatcgtt caaattcaaa
     9961 aaaaagcact atcgaaatcc gtgataaagg catcttcccg gtgtctaccc ctctcgaagt
    10021 cgcaaattat gccaatgaaa ttttagaagt aatcaagaaa ttctcataat aaaaaaagcc
    10081 ctctttacta tacccatttt atcaaaaaag tgaggtaaaa tcaatgtgga tggaagaatt
    10141 gccaaacggc aaatataaat tttttgagag atacaaagat ccatatactg agaaattaaa
    10201 aaaagtctca gtaacgatgg agaaaaaaac tccccaagca agaaatcaag ctgcgatttt
    10261 acttcaggaa aagataaaac aaaagttagg agaaaaacaa cattctgttt ctaatataac
    10321 ttttgaaaaa ctatatgaag aatttgagga gaattggaaa cacggcgtaa aaaactcaac
    10381 cgtctacgct tcaaagaatg taaaaaaaga gattttaaag cagatagagg gcgactacct
    10441 agttagaaat ttgatagacg tttattacaa aaagtaatag atcaactatt acaagatggg
    10501 agatctcata actatgtttc taaaatcaaa ttcaagctca atcagataat gaaatttgct
    10561 atcagaatga attatattga tacaaatgaa atgctatttg ttgaaacgcc tagaaaagta
    10621 attacatccg acgaactcag aaagaaaaat acaaaatact tagaccaaaa agagtttaag
    10681 ttattcatcc aaaatttaaa agacgaggcc ctatgtgatt atcgaattac aaagtatatc
    10741 cgaatagcta aagttctttt tcttactggc atgaggtatg gagagctggc agccttaaac
    10801 tacaaggaag atatagattt ttctaaaaag actattcaca tcaagcatac atacgatttc
    10861 agacaaaaag agagaactac accaaagaca atcaagtccg atagggttat aacagcacct
    10921 caaaaagtgt tagatattat caaagagcaa ataatagaga atgcgacaaa tggatttgat
    10981 acagatttta ttttcatcaa tactctagga gaaccaataa caaatgccag ggttatttgt
    11041 gcattgaaaa gacatggtca aaaaatcggc atagaaaaaa acataactac acatacattt
    11101 agacattctc acatatccct acttgctgag ctgggcattc ccttgactgc catcatggac
    11161 agagtagggc atagtgactc aaagaccaca ctagagattt attctcacgt tactcaaaaa
    11221 atggtatcag acatatctag caagttagac aagataaaat tttaaattgt gcccctcgtc
    11281 tgcccctttt tcttatacaa gacaaacaaa aaccccttaa agtgttgatt ttaaagggtt
    11341 tttaaagtgc acgaaaaaag agcacacaat tcaaatcgct tagggctgct ggattcctcc
    11401 cctgacccgc ttcacgcaga actgttgctc cactatttat tatatcacat tcctattcat
    11461 tttaaaagca aaattatttt ttccgtctat ttctaaaaaa atcctgcatg atagctgcgc
    11521 attcatcttc caaaattcct atttcaacct ccacacgatg attgagacgc tcatctgtca
    11581 agatatcgta caaactccca gcagcgccaa atttctggtt tttagcccca tagaccacat
    11641 ttggaatgcg ggcaagccca atcgccccac tacacatgac acaaggttca atggtcacaa
    11701 aaagtgtgca atccagcaag cgccagctct cctcactcaa gttcgcatcc tctatagcca
    11761 taatttccgc atgcataacc gctcgctgta attcctcacg cgcattatgc ccacgaccaa
    11821 tgatttcccc atctttgaca atcacacaac caattggaat ttcatcgtgt tcaagagcaa
    11881 tctcagcctc tctcaaagcc tccctcataa agacttcttt ttcttcaagc gtataataca
    11941 tctcttttct cttttcctac ttatcgattt tattattata tcatgaatcc caacacaaaa
    12001 gaagccaccg aatgcggtga ctctataggg agattattat gaaaaagaaa agtttaggat
    12061 atttgttaca acaagttagg aggtcttctt gtaactgtct atagtatacc cgacctatct
    12121 taaacaaatc ttaaaaatct cttaggacca aacactttct aaaatatttg tttgttcacg
    12181 accaggacct actgagaaag tagaaatacg aacgccaacc aattcactca cacgacgaac
    12241 atagttacgc gcattctcag gaagatcttc caaattgcga actccggtaa tatcttctga
    12301 ccaacctggc aactcttcat agataggctt gcaacgtttc aattgctcaa gactagctgg
    12361 atagtagtca atacgttgac cgtcaagatc ataggccaca cagattttca cagtatccaa
    12421 accgctcaaa acatcaatag agttcaaaga aaggttagta ataccagaaa cacgacggct
    12481 atgacgcatc acaactgagt caaaccaacc tacacgacgt ggacgaccag ttgttgtacc
    12541 atactcatga cccacttcac ggatacgttc tcccacttca tcaaacaact cagttgggaa
    12601 aggaccatct cctacacgac tcgtataagc tttacataca cctacaacct tgtcaatctt
    12661 gcttggaccg acaccagaac caattgtcac accaccagct acagggtttg atgacgtaac
    12721 aaatggataa gtaccttggt cgatatctag cataacacct tgtgcacctt caaaaagcac
    12781 acgtttgcca ttatcaagcg catcattcaa gataacagat gtatctatca cgtatttctt
    12841 gatttgttga ccatattcgt aatattcttc aaaaatatca tcgaaaacaa tcgctttact
    12901 gtcatacaat ttttcaaaaa gacgattctt ttcagcaagg ttacgttcta aacgctcacg
    12961 gaaaatatct ttatctaaaa gatctgcaat acgaattcca acacgagcag ccttgtccat
    13021 ataagctgga ccaattccct taattgtcgt accaatctta ttgtcgccct tagcttcttc
    13081 ttgcaagcga tccaactcga tatgataagg caaaataaca tgcgcacgat cagaaatacg
    13141 caagttatca gttgttacac cttcctcatg aagatagctc aactctttta caagagattt
    13201 aggatttaca accataccat tcccaatgac agatattttt tcagggaaga aaatcccaga
    13261 tggaatcaag tgcaacttaa atttcttacc gtcaatcaca atcgtgtgac cagcattatc
    13321 accaccttgg taacgtgcaa tcacttctgc attcgctgaa aggaagtctg taatcttccc
    13381 tttaccttca tcaccccatt gggtacctac aacaacaact gaagtcataa ttttgtctga
    13441 gccctcaggc tcttcctttc tcacatacat ggcaggactc tcacctgcaa ttatatctta
    13501 caatttatta taataaaaaa tcgccttttt atcaagaaga aacaataga
//
LOCUS       DAVLVF010000038        13442 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVLVF010000038 DAVLVF010000000
VERSION     DAVLVF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13442
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.038"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            102..1280
                     /locus_tag="VYN66_001199"
     CDS             102..1280
                     /locus_tag="VYN66_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1495224.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLISQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            complement(1508..1954)
                     /locus_tag="VYN66_001200"
     CDS             complement(1508..1954)
                     /locus_tag="VYN66_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1495225.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGFIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSILKLE"
     gene            2024..2683
                     /locus_tag="VYN66_001201"
     CDS             2024..2683
                     /locus_tag="VYN66_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV1495226.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWKLLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTCHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            complement(2735..3886)
                     /locus_tag="VYN66_001202"
     CDS             complement(2735..3886)
                     /locus_tag="VYN66_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV1495227.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(3901..4632)
                     /locus_tag="VYN66_001203"
     CDS             complement(3901..4632)
                     /locus_tag="VYN66_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495228.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            4861..4962
                     /locus_tag="VYN66_001204"
                     /pseudo
     CDS             4861..4962
                     /locus_tag="VYN66_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            complement(4934..5710)
                     /locus_tag="VYN66_001205"
     CDS             complement(4934..5710)
                     /locus_tag="VYN66_001205"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV1495229.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(5703..6926)
                     /gene="trpB"
                     /locus_tag="VYN66_001206"
     CDS             complement(5703..6926)
                     /gene="trpB"
                     /locus_tag="VYN66_001206"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1495230.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            complement(6904..7503)
                     /locus_tag="VYN66_001207"
     CDS             complement(6904..7503)
                     /locus_tag="VYN66_001207"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV1495231.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVVADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            complement(7490..8257)
                     /gene="trpC"
                     /locus_tag="VYN66_001208"
     CDS             complement(7490..8257)
                     /gene="trpC"
                     /locus_tag="VYN66_001208"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV1495232.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLNYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            complement(8254..9258)
                     /gene="trpD"
                     /locus_tag="VYN66_001209"
     CDS             complement(8254..9258)
                     /gene="trpD"
                     /locus_tag="VYN66_001209"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV1495233.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDVRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            complement(9269..9835)
                     /locus_tag="VYN66_001210"
     CDS             complement(9269..9835)
                     /locus_tag="VYN66_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV1495234.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            complement(9832..11193)
                     /gene="trpE"
                     /locus_tag="VYN66_001211"
     CDS             complement(9832..11193)
                     /gene="trpE"
                     /locus_tag="VYN66_001211"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV1495235.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEVMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNSFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGVTDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            complement(11740..11817)
                     /locus_tag="VYN66_001212"
                     /pseudo
     CDS             complement(11740..11817)
                     /locus_tag="VYN66_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     misc_binding    complement(11983..12212)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(12424..13425)
                     /locus_tag="VYN66_001213"
     CDS             complement(12424..13425)
                     /locus_tag="VYN66_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1495236.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT         3883 a         3017 c         2314 g         4228 t
ORIGIN      
        1 accagtaagc aatgaaaagc ttaaaaagat taacatctac atttatccat agcaaaaaat
       61 cataggattt taagaatctg acaactagga agaaaactaa tatgacctta tctaagaaac
      121 aattacaact acgagctaaa atactggaaa cagtttacac attaggtccc atttcacgaa
      181 ttgaaatcgc tactaaaaca ggaataacac ctgcaacaac tagcagtatc acaaatgatc
      241 tgattaaaga aaacattctc ttagaacttg gtgaagacga acacgataca agcgtaggga
      301 gaaaaaaaat cctgctagac attcaagcta aacgatttta ctacattggt tgtgaactat
      361 ctgaaaaaca tttcactttt gctttaggag ataatctagg aaatatctta aaggaagaga
      421 aagaaattgt tactaaacag ttaatccaag aaaagggaaa tcaactcatt agccagacac
      481 taaagcaatt cttaaataac tgctctgatt atgaaataga agcaattgga atcgcgctac
      541 ctggtcgcta tttagatgat tataaaataa caacaaacaa tcctttatgg caacatatcg
      601 acttagaaat gattcaatcc cactttgata agcctttgtt tttctctaac aatgttaact
      661 gtatggctat aggtaaacgc ctatttagtc gccaacagaa tgaccctaat tttgcttatt
      721 ttcattttgc tagaggtatg cactgttctt atatctacga cggaaacatt tatggaaaag
      781 gaaatctaat gattggagaa atagggcata cagttgtttc atctgaggga gaagaatgta
      841 gttgcgggcg caagggatgc ttacaaactt ttgctggaga atcttggtta atcaaaaaat
      901 caaaaattct atatcatcaa tctccctatt ctctacttcc tagtttagtc aaaaatgccg
      961 atgatattga tatccaagtt attttaaccg cttatcaatt aggtgacact ggaataatca
     1021 cacttatcca tcaagcctta ctctatctta gtcaaaccat tttaaatatc agtatgatga
     1081 ttgactctca aaaaatctac ctacatagtc ctttactaac aaatcaacac atcatccaaa
     1141 aactatatag tgaaatgaac tacaaaccca aactgctcta taatcgccta cctgaagtca
     1201 ttattgaacc ttacaatgat tttacagccg cacactcagc tattgcactt tgtttgtatc
     1261 atacaattct acattcctaa ttttctatta catttaataa ataggctaaa aagagggagt
     1321 atatcataaa aagcgaacaa acctgttttc tgaaaatcag aattctgttt tgttcgcttt
     1381 ctttatattt ggaattatac tcctatctcc cctttttcat aatgtatagt aaaatgaaat
     1441 aataacagga cgaatcgatc gggacagtca aatcgatttc taacaatgtt ttagaagtag
     1501 aggtgtacta ttctagtttc aatatactat attttcgtac aggtgcttca accatttgaa
     1561 cgatttcaaa tccttctttt tggtaaagat tctgagctct ttgatttgcc tcgaagacat
     1621 ttagagaaat actatctata tcttcatttt caaaggccaa actaacaaat ttccttaaag
     1681 cctggctacc taagccttgc tcctgtttct gggggttgat aaaaaatctc ccgatatgaa
     1741 gattgctgtc ttctagcctg attttctgga taaatcccac aaactcttgt tcatcaaaga
     1801 ttgaaaagac tccttccaag gcttgaaggg tcagtagaaa aggaatcctt ggtcccatcc
     1861 attgttcttg aaaggatttg cctagggagt tggaccactg gcatacaaat tgagcgtttt
     1921 ctatactcac cttttcttca aaacgaattg tcatcttttc ctcaccatct tatctatgtt
     1981 tctccattat actatttctc ccatttttta cgaatagata agtatgattg atttttattt
     2041 ttttctcgtc gggagcattc tcgcttcctt tcttggtttg gtcattgacc gttttccaga
     2101 gcaatccatt atcagttcag ccagtcactg cgattcctgt cagactcgct tgcgtccctt
     2161 agatttgatt ccgattctct cacaggtctt caatcgcttt cgctgtcgct actgcaaagt
     2221 tcgctatcct gtctggtatg ccctctttga attagtctta ggactcctct ttctgcttta
     2281 ctcttggaaa ttgctttcct tggggcaagt cgtcctaatc accgctggtt tgaccttggg
     2341 tatctacgac tttcaccatc aggaatatcc cttactggtc tggatgactt gccacctaat
     2401 cctaatagct tcctctggct ggaatctggt catggtctcc ttcctcatac ttggaatttt
     2461 ggctcatttt atcgatatcc gcatgggtgc aggggatttc ctttttttag cttcttgtgc
     2521 tctcgtcttt agcgtaacgg agttactgat cttgattcag ttcgcttctg cgacgggtat
     2581 cctggccttt ctcctgcaaa agaaaaagga aagacttcct ttcgtgcctt tcctcttact
     2641 tgctacttgt ttgattattt ttggtaagct actgcttgtc taataaaatc caatttctgc
     2701 catatgtcct tcatgaaatt atttcacagt taaattataa attatttctt ttgtacaaag
     2761 ggatgatgtt atcaaatcga tctgttcttc tatcttcttg atactgatca aaaaatttca
     2821 tttcgactga aaatatttcg cttataaact gtaaacgaat actttgttta gacattatag
     2881 tcgctagact gactagatga ttactcaaaa cgacgtccag aatactcttt actttgcttg
     2941 gttttttaac aaaaatttga tcatccaaag gttcaatcat tttgtaacct ttttgcgcaa
     3001 tttgacgata aaagtaagaa tgttgctttg gagtcaataa tcctaactta aaagctcgat
     3061 actctaaagc ctgtatcgaa acattcaaat ccgacttcaa taaaatataa ctatcaggat
     3121 tgctgacacg cttgccaacc ctctcttcaa atttgactaa aaactcttct tttggcaata
     3181 aaaaacatga tgcaaaataa tttgcttctt gctccaaacg atcgccatct tcattcatat
     3241 ctttatattt atgtaaaaga atatgtccta gctcatgagc taagtcaaaa tttcgacgta
     3301 cagatgattt attcgttccc aacacaatat aaggtcttcc caattttgac catgcgctat
     3361 aagcatcagc ttggccatta attaatcgtt ccacgatata gatgcctgaa cgttctaatt
     3421 tataaagcaa atcatgatta tcttttgaaa tacctaattt ttccctggca taaagagcca
     3481 attcctcaat ggattctccc ttatgataag attcactcac tacattactt aggtcatgaa
     3541 ttgtaatatt aggtataatt acaaaacttt caaaataatc aatcaaacta tctaccttat
     3601 gtaaatacat agtttgaata tctattgttt tccgtgttgc taggtctgca tttctaaagg
     3661 caattacaga agaatcaaat cgaatgctct cttcttcctg ttcaaaataa gttaaatcaa
     3721 catgaaattg gttggccaaa tgcattttgg ttgataattt aggtttcgtt tcgttggact
     3781 caaactgcca aatggcttgt tccgttaaat taattctctg agctaattct gctctactta
     3841 aaccatttaa cagccgtaat tctttcaata cccgaccatt aaacatttac atactcctta
     3901 ctacttttga ccttcttgtt tttctattct tggaataatt tcaaaatctt ctgtttccga
     3961 taattctgaa aaattaggaa tatcttggta tttagcttct tcgaaatggt acgaacttga
     4021 ttgaatatac tcagacaaat cttgaatcaa gtgtaactga ccagttcgtg catcaggcat
     4081 aacaacatct acagatataa tattgttttc tgagtccgcc tcataagtta aaatcataaa
     4141 tttttcgata ttcgaatttt tggtagcttg ttcaatttct tgaatcattt catcagaaac
     4201 taactccatc tgaattggaa aggaatgact atcttcatca tttttgtagg aagaatgttg
     4261 attaagataa agtgtattca tttgagcata ttcaaataag tagccactct tatttttttg
     4321 taccaaagga aattggttcg taagtcgctt cttacccttt ataattaaca atactttccc
     4381 atatttttct gtatttgttt caaattctaa atatccccaa gtctgtcctg ctaattgtaa
     4441 tttatactca aacaaatctg ctgatgcaaa tgcagtatca atatgattag gtcgcgtcca
     4501 tgcataacca ttcgacacta tcattgtctc tcttttttct agacgttcat ctacataatc
     4561 tttttgccct ttcatcaaag tatctacaat tttttgtgcc tcaagcgaat caaagagatc
     4621 ctgattcaac ataattcttc ctcctccaaa tactttttaa tgaattatat cattttctta
     4681 aagaaattac tacaataatt agctttttct taaagttctg tgtcagagta atttagaaaa
     4741 ttatatcttc tatagtaaaa tcaattaaaa actgaacaaa tttattggga aattcaaatc
     4801 actttcttaa aatattttag gaaccgtagt gtaatattcc agattcaatt cactataaaa
     4861 ctagcctttc tcctgcaaaa gaaaaaggaa agacttcctt tcgtgccttt cctcttactt
     4921 gctacttgtt taattatttt tggtaagcta ctgcttgttt gataaagtcc tgaatcggct
     4981 ctccttggtg gagagctttt actattttcg aaccgacgat aacaccatct gacaccgcat
     5041 tgaagcgttc cagatcggct tgactagata caccaaaacc tgtcaagact gggatgtcgg
     5101 ccacttgatg aagttgcgcc aagtgcttgt ccaaatctgc acggtaattg cctgatttcc
     5161 ctgttacccc attgatggca acggcataga cgaatccctc cgccccttca atcaactctt
     5221 tctggcgctc aattcctgtg gtcaagctta ctaaaggaat caaggcgata tctgtatctg
     5281 ccaaaaatgg ttctacaaag ttggcatgtt catgaggcag gtctgggata atcaagccct
     5341 tcacagctgt atcagccaga tctttgacaa agttctccac accgtactga aagagggggt
     5401 tgaagtaggt catgatgacc agtggaatct ctgtttcaat ggttttcaag gtttcaacta
     5461 aagcctgggt agaggtcccg tgggctaaac tgcgcaagcc agcttcttcg ataacaggtc
     5521 catctgcaac agggtctgaa aagggaatac ccacttcaat tgcagagaca cccaaatctt
     5581 ctaaaaagtg aattgtttca gcaagaccat ccaaaccttt ttcgtggtca ccagccatga
     5641 tatagggaac aaaaattcct tttccagctg ctttaatagc atttaatttt tttgttagtg
     5701 tcttaggcat gagcttctcc cttctttgct gcatctgctt ccaagcggtc ttttacttga
     5761 accacatcct tgtccccacg acctgatagg catacaatca tagacttttc tggtccaagt
     5821 tctttggcca atttcaccgc aaaggcgata gcatggctag attccaaagc tgggataatc
     5881 ccttccacac gagacaagag ttggaatcct tccaaggctt cttcgtctgt cacagggaca
     5941 tagctggcac gtttaatatc gtggtagtga gaatgctctg gaccgatacc aggatagtcc
     6001 aaacctgctg agatagagaa ggcttcaaga atttgaccat gggcatcttg gagcacatcc
     6061 atgagggaac cgtgaaggac acctggacga cccttggtca aggtagctgc gtggtgctcc
     6121 gtatccacac caaatccagc cgcttcagct ccatacatgg ctactgactc atcttctaca
     6181 aagggatgga agagaccgat ggcattcgac ccaccaccaa cacaggctac tagggcatct
     6241 ggcagatttt gtcctgtcaa gtcacggtac tgttgtttag cctctcgacc aatgacactt
     6301 tggaagtcac gaacaatttc tggaaatgga tgaggcccca aggcagaacc aaggatatag
     6361 tgggtatcat ctatattagc cacccatgaa cgaagggctg cattgaccgc atccttgagc
     6421 acgcgcgaac catctgtcac ggcctcgacc ttagctccca aaagctccat acggaagaca
     6481 ttgagggctt ggcgtttgac atcttcctca cccatgtaga tggtacattc catgttaaag
     6541 agggccgcag cagttgcagt tgccacaccg tgctgaccag caccagtttc tgcgataatt
     6601 ttctttttac ccatgcgttt ggcaagccaa acttgtccca aggcattgtt aatcttgtgg
     6661 gctcctgtat ggttaaggtc ttcccgtttg agataaatct tggctccgcc gatatgctgg
     6721 gtcaagtttt ttgcgtaata aagaggagtt tcacgtccta cgtactggcg caaaagctgg
     6781 tttaattcct cttggaaact tgggtctgcc tgactttcac ggtaggcctt ctccaactcc
     6841 aaaactgctg tcatcaatgt ttctgggaca aaacgtccgc cgaattttcc gtaaaatcca
     6901 tctttatttg gttcctgata tgccatgctt taccctctct ataaatcttc taatcttttc
     6961 atgatctttt tgtccatctg tctccactcc gctcgataca tctactgcat agggagtaaa
     7021 atgttgaatt gcttttacta cattatcttc attaaggcca cctgcgataa agaagggctg
     7081 tgctagtcca gtcgtatcca gttgacccca atcaaaggac tgaccacttc ctgccacagg
     7141 ggcatcaaag agtagataat ctgcctgaga attggggaca tgcccatttc catctacctg
     7201 cacagcctga atactggcac aaggcaaatt ctcaaataaa tcagctacca cctgaccgtg
     7261 aacttgaacc aagtccaagc caactttgtc aatcgcttcc agcagttcta cccgacttgg
     7321 tgaaacaaat actccaacct ttttcacatc tgcaggaata agctttgcca actcagctgc
     7381 ctcttctaaa gtcacctgtc ttttactagg tgcaaagaca aaaccgatat agtcggctcc
     7441 tgctgaaacg gctgtttcca ccgcttcttt ggtcgatagt ccacaaattt taacctttgt
     7501 caatctgcaa ctccttgatt ctctgggcca cattttctgc ctgcataaga gctgtcccta
     7561 ccaaaattcc gttaaagtat ggggctagtc gttccgcatc ctgccctgtg aaaatggcag
     7621 attcagaaat gtaatagcga ccttcctcaa agtaaggggc taaatctaca ctggtctgca
     7681 agtcgacctc aaaggtagtc aagttgcggt tgttgacccc gataatctca gcaccaagtc
     7741 tgtgggctac ctctagttca gctagattgt gagtctccac taagacttcc agaccaagct
     7801 ctgtcgcgta gtcatacagt tccttgaggc gttcttcgga caaggctgcc acaataagca
     7861 agataactgt cgcacctgca ttgcgagcgc ggatgatttg cttttcatct atgataaagt
     7921 ctttgttgag cgtcggaatc tctacctgac tggaaatttc ccgtagataa ttcaaatgcc
     7981 ctttaaagaa aacctcatct gtcaacaccg aaatcatcac tgctccgttt tcttcataag
     8041 tctgggcctg ttgcacaata tccacatcga gattgatatc tcccaaacta gggctagctt
     8101 tcttgacctc agcgattacc tgcaagcggt cctgatgatt cttcaaaaat tctgccaagc
     8161 gataggtctg gcgcagaggc tggatttgct ccagcttcat ttgctccacc tcacgcgcct
     8221 tctgctctaa gattcgtgct aaaaattcct gactcatttt tgatactcct gtaacagtct
     8281 gagtttttca agggccttgc ctctagcaat cacttgacgg gccaaggcaa ctccttcctt
     8341 gatgctatca atcttaccat tagcatagaa accaagacca gcattcaaga ctgtcgtttc
     8401 caagaatgga cttgcttcgt ttttcagaac gctaagcaaa atttctgcat tttcctgagc
     8461 attcccacca cgaacatctt ccatagcata gccttccatt cccaaatcct ctggagtaaa
     8521 gcttgacaag ctgatttcgc cattttcaag aagtgcaatc ttggttgttc cgttcaagcc
     8581 agcttcatcc aacccttctg gtccagcaac cacgatggca cgtttgcgac ccatattttt
     8641 caaaacctga gctgtacttt ctaggagttc tggacgacta attccaagaa gctgtgtttc
     8701 taaagccatt ggatgaatca gtggaccagt caagttcata atcgttggaa ttcccaattc
     8761 caaacgagct ggcatgatgt atttcatagc tgggtgcata tttttagcga agagaaagac
     8821 gattccagtt ttatcaaaga ccttacctag ttcagctggt ttgaggtcaa gattgattcc
     8881 caaggcttcg aggacatctg cggaaccaga tttagaagaa atcgagcggt taccatgctt
     8941 ggccatgtga ataccgccac cagccaagac aaaggctgca gttgtggaaa tattaaaact
     9001 gaaagacttg tccccacctg taccacagtt gtccatggca tcatgaatct cagttggaat
     9061 atgctgggca tgtcctctca tgacttgggc aatggctgtg cgttcttcag gtgtttcccc
     9121 cttcatctta agagctaaga ggagagaagc aatctgcgct tcagttacac gcccagttac
     9181 gatacgctca atgacatccg tcatttccac acctgataaa ttttcaaatt ttgctagttt
     9241 ttcaataatc tctttcatcc tagtttcctc actttacaac cttctcgata aaattccgaa
     9301 tagaagacaa gccgtctggc gttccaatgc tctctggatg gtactggaag ccataaatcg
     9361 gtaggttttt atgttgaatc cccatgatgg cttggtcatc agtcgaacga gctgtcactt
     9421 caaagtcttc tggcatttcc tcaatcaaaa tactgtgata acgcatgacc gcacgtccat
     9481 cctcaatacc ttgatacaaa acagatggcg cttcaaagtt gatattgctc tgtttcccat
     9541 gcatgacttt tggagccaaa cctagcttac caccaaagac ttctgcaatg gcttggtggc
     9601 ccaaacaaat ccctagaatc ggcttcttgc ctgcaaaatc acgaatcatg tcttccatct
     9661 ttccagcatc aactggccaa ccaggaccag gagaaaagac cagaccatct gctttttcag
     9721 cttcttcata cagcttggaa tcatcatttc tcagaacctg aacttctgca aaattcccaa
     9781 tgtattgggc caagttatag gtaaaagaat catagttgtc aatcaataaa atcatggtct
     9841 tagttctcca attctagtca tagattttgc tttgttaatg gtttcttggt attcgttttg
     9901 ggcgatagag tcgtagacaa tccctgcccc agcctgcaca taggctcttt gatttttgag
     9961 aatcatggtt cggatggcaa tggccaaatc catatcaccc gtcgcagaca agtagccgat
    10021 tgctcctgca tatacgcccc gtttttccgt ttccagttca tagatgcgtc tcatcgcccg
    10081 aatctttggt gctccagaaa cggttccagc aggaagcgtt gctttcaagg catccatggc
    10141 agtgagttct gcaagcaaac gccccttgac cacgctggtc aaatgcatga cgtagcggaa
    10201 gagctccacc ttcatatact tagtaacttg gacactggcc gtttcagaga tgcggccaat
    10261 atcgttacgc cccaagtcta ccaacattcg atgttctgct gtttccttct catcagagag
    10321 gaggtcagtc gccaaggtct tgtcttcttc atccgtaacc cctcttggtc gcgtccctgc
    10381 aatcggattg gttgtcacga tgccattttt gacagaaact aaactttctg gactagctcc
    10441 gatgatttga taatccccaa aatcatagaa atagaggtaa ttggatggat tggtcacgcg
    10501 gagatttctg tagaagtcaa atgaatttcc agtaacttct gctgagaaac gctggctgag
    10561 tacacattgg aacatatcac cattacgaat caagtcacga gctgtttcta ccattacctc
    10621 aaacttatgt ggagcgatat gcggtttgaa gtcaagtggt gataaatcca agtcttcaaa
    10681 ttcatttgga gcaggaatgc gtaattcctc aagcacttgg ttcaaggatt tttccaaggc
    10741 ctcttgactg cgctcactat agagagcatc ctctatgaca tgtatcttct ccttcttgtg
    10801 gtcaaagacc atatagctct catagacaaa gaaatgcatg tctggcgtcc caattgtatc
    10861 ctcagggatt tgaccaattt cttcataaag cgaaatcata tcgtaaccca caaaaccaat
    10921 ggctccacca ccaaaaggta gctctgagtg gtgctgactc ttatgaatca cttcataaag
    10981 gaaatccaag ggatctcgat caatcacttg accattttga taaagaactc cattttcaaa
    11041 cttaatctca aaaacaggat tataggctaa gatagaaaaa cgagctgttt ccttgtctct
    11101 cggaatactc tctaagataa ccttgtgttg cccctttaag cgcatataag ccaagattgg
    11161 tgataagaca tctccatgaa tgattcgttc cattgtaatt tccctttcag ttctacttct
    11221 agtccgtggt gactgtatga aaaatcccca cgcaaaataa cttgcgtgag gacgaaattc
    11281 gcggtgccac ctcaattata ggatttctcc tatctctcat tcctgtctca gatacctcct
    11341 gtaacaggct gtgcgataaa gggcactccc ttgagaatga tgttttcttc tctcgtttca
    11401 gatgaaccca actttacagc tttctctgct tgttttcagc aaccacaagc tctctgtgag
    11461 agaaaagact gtaatttttc catctattat tttttagctt ctaggtaatc tgcaatcgca
    11521 gctaggtcct tgcctccacg accagagata ttgatgagga gatttttatt tcgatacacc
    11581 tttatactct tcgaaaatct cttcaaaccg cgtcaacgtc gccttgccgt aggtatggtt
    11641 actgacttcg tcagttctat ctgcaacctc aaaacagtgt tttgagctga cttcgtcagt
    11701 tctatccaca acctcaaaac agtattttga gctgacttcg tcagttctat ccacaacctc
    11761 aaaacagtgt tttgagcaac ctgcggctag tttcctagtt tgctctttga ttttcattga
    11821 gtattactag cttttttcgt attagtccag ctttttgttt gcttttagta gtaggcaagg
    11881 agctgtagat agaactcaag ttcatcaaag cgacttaagg ccctaataaa agataaacca
    11941 aacgacggat agaaaaaagc ccacacacaa aatatacttc cgtgtgaggg cgttggtaac
    12001 gcggtgccac ctcaattata aagggactat ccctttacat ctctgccttg tttaacaaca
    12061 agctgcactg taaggtgtgc gcaccgaatt ttcattgttt caaattcatt ttcaaaatca
    12121 gcccactttc actacttcca accacctatt cacaaccacc acaggctccc tgaagatcaa
    12181 aaatagttac ttttctgatt tgttgaactt attttaatac tttgtttttt ctttgtcaag
    12241 acttttttac gatttttttg aaaatatcat tcgaatgtga ccatgtcttc cttagatcga
    12301 acatgaacat gtcccacttg ttagaaattg gatccaactc aatagaaact gaatggaggc
    12361 taaacagaac ttattttaga acactccatc ttttccacta ggattttcaa gaattaaaca
    12421 atactagaaa ctctgtctcc taacaaattt aggagaaact tcaacagatg tgacactttc
    12481 ccctttaata attgctaaaa caccttctat catttcttta gccaatttaa cataattggg
    12541 agcaattgta gacaaagctg gagtataata ctgagaaata ggaatattat caaatccaat
    12601 gatagaaata tcatctggaa taagaattcc tttctcatag cacgcacgaa tcaagccctg
    12661 aaccttttca tctcctgaaa caaaaataat gtccggataa ttttgggtag tcaagtgctg
    12721 cattgcataa gaataaactg aatcaattgt agataagcca taaatgactt ttaaatccat
    12781 aaagtaattt ttatcattca gaaaagaacg cacacctctt tcacgatcct tattaacatg
    12841 ggattctcct cccataagca accacatatt tttaaatttt tcttcagtta cagctttcat
    12901 catatcataa gtagcttgaa aattattatt agatacatag actactccag acgtttgaga
    12961 ttcaccgaaa acaagaaaag gcatatggtt cttctttaaa tactgaattc tgatatcatc
    13021 tacactttca taaaaaacaa taacaccatc tactaggcta cctgtgcttg atataattga
    13081 attactaatt gtatcctcct ctccaaagta ctcaactata gcattaacac caaattcttt
    13141 acacgtccgt aacactttat ctaacagcgt atgaaaccaa attaaaggaa aagagtcgat
    13201 tttttttaca gaaatcaata tatttatagc ttctttttta gttaaatttt ttgcatacgc
    13261 atttggaata tacgacaatt cctctataac tttttgaatc gcttgataag tttcttcttt
    13321 aacatttact ccaccattaa taactcgtga aactgttttt ggagaaaaac ctgataaacg
    13381 tgcaatatca taaatagtta cctttttccc atttatattt ttcatttcag tcctccatta
    13441 cg
//
LOCUS       DAVLVF010000039        13141 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVLVF010000039 DAVLVF010000000
VERSION     DAVLVF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13141
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.039"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            130..312
                     /locus_tag="VYN66_001214"
     CDS             130..312
                     /locus_tag="VYN66_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1495237.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            257..968
                     /locus_tag="VYN66_001215"
                     /pseudo
     CDS             257..968
                     /locus_tag="VYN66_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1038..1400)
                     /locus_tag="VYN66_001216"
     CDS             complement(1038..1400)
                     /locus_tag="VYN66_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495238.1"
                     /translation="MPKTKTLAELADVILWGFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1778..3658)
                     /locus_tag="VYN66_001217"
     CDS             complement(1778..3658)
                     /locus_tag="VYN66_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1495239.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVMGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAGTIADIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3652..4521)
                     /locus_tag="VYN66_001218"
     CDS             complement(3652..4521)
                     /locus_tag="VYN66_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1495240.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLYRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4605..7250)
                     /locus_tag="VYN66_001219"
     CDS             complement(4605..7250)
                     /locus_tag="VYN66_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV1495241.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEVTIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(7341..8621)
                     /locus_tag="VYN66_001220"
     CDS             complement(7341..8621)
                     /locus_tag="VYN66_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV1495242.1"
                     /translation="MIYPKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8792..10876
                     /locus_tag="VYN66_001221"
     CDS             8792..10876
                     /locus_tag="VYN66_001221"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV1495243.1"
                     /translation="MKPLLETIDTRFGTASKHAFSWGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLVSSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKSSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKILVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(10916..12595)
                     /locus_tag="VYN66_001222"
     CDS             complement(10916..12595)
                     /locus_tag="VYN66_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1495244.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYNQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQQVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELDYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGENAYFTVKRRECSGPLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQAGETEKSLTLPTLYFAGDKTLYFYLNLTVDGQLVDQLQVQVS"
BASE COUNT         3665 a         3111 c         2649 g         3716 t
ORIGIN      
        1 ttaaaaaaga aattaaagcg ggttaaaaaa gaaattaact gagtaaaaac tctcgaaatt
       61 caattcgcta tattatcact caacttgaac aaataccaga accacaaaaa acagatgtaa
      121 aggaaaaata tggacttaaa atttgaaggg gttgatttag aatacaaaaa agctaaaaac
      181 aatcttccag agtctttctg ggaaacctac tcagcatttg caaatacaaa tggcgggaaa
      241 ataattctcg gtatagatga aaaaaacatt gacacctacc aaagagtcaa tagactccct
      301 gcgaaattat gaagcaagta aacaattaac tgatacacga tttaagcgtc ttgttggtgt
      361 tcagcgtacc acttttaaag agatgttagc tgtgttaaaa acagattatc aacttaaaca
      421 cgcaaaaggt ggacgaaaac ctaaattaag cctagaagac cttcttatgg ccactcttca
      481 atatgtgcga gaataccgca cttatgaaga aattgcggct gattttggta ttcacgaaag
      541 caacttaatc cgtcggagcc aatgggttga agtaactctt gttcaaagtg gtgttacgat
      601 ttcaagaact cctctcagtt ctgaggacac ggtaatgatt gatgcgacgg aagtacaaat
      661 caatcgccct aaaaaaacaa ttagcgaatc attctggtaa aatcttggct gacagtgatt
      721 atcaagggct catgaagata tatcctcaag cacaaactcc acgtaaatcc agcaaactca
      781 agccgctaac agttgaagat aaagcctgta accatgcgct atctaaggag agaagcaagg
      841 ttgagaacat ctttgccaaa gtaaaaacgt ttaaaatgtt ttcaacaacc tatcgaaatc
      901 atcgtaaacg cttcggatta cgaatgaatt tgagtgctgg tattatcaat catgaattag
      961 gattctagtt ttgcaggaag tctattgaaa tactaagcct acacagactg attccaattg
     1021 ttttttagat ttcaatttta ccattcttca tagtcaaaac catcataatc tggatattgt
     1081 tctggcgacg ttccaaccga acgtacgaga taagcttctt cgtcctctgt ctcgatttct
     1141 ctcagtactt ggcattcaaa acgccattca tcaccgaagt cgaaaataaa cttaaatttt
     1201 tgtttgacac ttaggctatc cagatagaca ttttctgtgt aacgttcttc aacatcgtca
     1261 ctaacaaagc taagaaagta agaatctgca tgactccact caacattatc catgaaaaat
     1321 gcgtgagcat gatcatttgc aaaatcaaaa ccccataaaa tcacatctgc tagttccgct
     1381 aacgtttttg tttttggaat ctatatatgt cggtagcaac cttttttaaa tttgacggaa
     1441 atgatccaag cttctacaga tgctggcttt tcagcttcta tttcttgttt tactaatttt
     1501 aagtgtagca taatacctcc aattgctcat cgatagaatt gctcttagga aacattcact
     1561 ttctgctatt ttatcataag tttgtctaac tgtagcattt gaaaccagat aattcttctg
     1621 tttcacgcga aacaccctgc gccaagcaag gtgttcttgt ttttatactc aatgaaaatc
     1681 aaagagcaaa ctaggaagct agctgcaggc tgctcaaaac actgttttga ggttgtagat
     1741 ggaagctgac gtggtttgaa gagattttcg aagagtatta agtcgtataa atcgtcgaag
     1801 ctgtcgcaat ggtatgccac tggttagctg tagttgctgc gaaatccttg tctgcgtaga
     1861 agtcagtaaa tggtaggatt tcaacttcca gctcgtcgat gcggtcaagc tggccagcca
     1921 gataaacctc gatacggctt tctgctcgtt tgatgcgttg caagagtccg cccatacgga
     1981 tgtcaactgt atccaaacca aagaccttgt tttctttcaa ccattggtga ctaaagaggg
     2041 catggaagtc ttcaatttgg cttctaagtt ctggtaattc ttgtctggcg atttgttgta
     2101 aactttcttt atcatccgct tggtaggcct gacgaatgcg tcgtcccaca tctactttgc
     2161 tacttaaaat agcattcaac tgggcctgag tttcaaagag ataagcatag tttccagctt
     2221 tttctttaat gtcagcaata gttcccgccg cctgagcgaa gtgtggcttg tcctgttcag
     2281 gtgtcatgtg tcggtcaagt atcggacaga gaacatcctg ataaaagaca tagcggttgg
     2341 gattgatgcc actgagatta cctggtaggt ctggtaagag gttggcaagg tcaatctgca
     2401 taaaatcctc aaccgttaga ccagtattgg tcttgaaatg cgcagacaaa ctatctaggt
     2461 cattgcgata gctgagttct gcccagattt gcaagcttgg taggatagag aactgggcag
     2521 tttcaccacc attgtctccc caacccatta cgatgacttc tttaatctga ttggcacggc
     2581 aggctttatt agcctcgata gccactagac ggctaaaatg gttgtgaggt gtaaagccaa
     2641 tccacttcca agctccccct gcaaatgcaa ggtcatggct aatcttgtga tgattgcgga
     2701 aattacggtt gtatttttcc tcgctatcct gataataatc ccagtaaacc agagtcacac
     2761 ggtctttgag acggtctagg tagacacgag tttcctctgg aatttccaca tcacgatcgt
     2821 actggccatc cgctgacatg agtttgaaga acatatcact ccacatctgg cagtggaaac
     2881 catatttgtc agcaatatcc agcacgcgct ccaagtgttg gcacatgagg agactacgat
     2941 ccacaacacc gttcaaaatc aggtagcgtc ccaaaccaac caagtgggct tcgtccatcc
     3001 cgatattgac cttgcgagtc ttcagtttag acaacgtggc aaacatgcca tcaatcaggt
     3061 cataaacctt ttcttcgcca atgagaagaa tgtcctctac atcacggagc tcctgcactt
     3121 ccttgacacc ccatttgaca aaggccgaca agtgggccaa ggtctggatg catggtacaa
     3181 aggtcatgtc aaactgttga gcataggctt cgatttcctg caactcctct gctgaataag
     3241 ctccacggaa atagccaaag taaggctgtc cttcaatctg gtaagtgtct tccatgtaaa
     3301 gctcaaaggt tgagtagccc atgagagcca agacctcaat catctgcttg gcagaagcca
     3361 cattcagcac cgcatttcga gaacagtcaa ccatgtaagc caaatcttcg taagctgctt
     3421 gttcctcaat ctctacttta tcagcttctg ctagaactgt taccaacaag gataaggcac
     3481 gataaagttg gtgaggtttg cggtaggtca attgatagtg accttcctca ccctcgatag
     3541 agatagaggc ttggtcagac tgagtgacag ccacttccac atctggtaga gaaatgtgac
     3601 cttttaaaac ctcaatagct tgcgtttgtt tgggactaag tcctgtaaat cttaccattg
     3661 cttttcctcc tgtagccagt tgacaagggc accgtagaga ttggcatctg cgtgataggt
     3721 gcaggcctgg ataactggtg cgaccgtgta ttcttcgtag gcatcgacaa agtcttcaac
     3781 agccttctta acaccttgga taaaatctgg attttgactg atagagccac ccagactgat
     3841 gacatctgga tcgatcagat actggatatt gagcaagcct tgcgtcagat tgcggttcat
     3901 gcgctcaatg gcttcttgac aaagagcatt accagctgcg gcctcttggt aaatcttgcg
     3961 accgtcccaa tcagtatgac cagatttttc aatcacgtat cgtaccatat tcccagttga
     4021 tgctagttgc gaccagttat taagtttttc agcaggggca agggttgtca tgtagccaaa
     4081 ttctccaccc agaccgtggc gacctcgata aagtctacca ttgataatca tggctccgcc
     4141 aatccctgtc ccaatcacga cacaggctgc attttcaagc tctggatgag ctagtagttc
     4201 actgagtcca acgcagttgg catcattttc taaatggaca ggtagctgat aagagctaag
     4261 cgcctcatac caagaaaagc catggatgta gggcaccgca ctgaagccat caatcacacc
     4321 tgtctcttga ttgaccgcac ctggaacgct catagcaatc ccactgtaat cctgttctga
     4381 caagcgttga tctagccacg ctagtaaatc ctccaagttt tcaggcgttg aaatacttgt
     4441 cttatccagt attttcccat caggagtcag actggcaaac ttaatcccag tccctccgat
     4501 atcaatcgtt gcaatggtca ttgtttttac cataaaaagg ggcgaatcag cagttagttc
     4561 ttactttttc gcccctcctt tcttttgatg tttacttttt gaaattaaat ttcttctttt
     4621 ttgatgaatt ctgtacgaat ctcttgtgga gccaatagtc ctgaatgaac tggatatggt
     4681 cgctcaagta agtcaagaat ggtttgttga tgttctgata tgcgcacatt ttcttgactc
     4741 atattatagt aacggaggac atagccttct tcattttccg ccactttaaa ggctgttgga
     4801 caaacttgcg gtatgctgag aacagaatgg ctcaagaggc taccagtcgc aaccacgctt
     4861 ccttcctgtc tagcaagctg aaggctggta aacggtgtct gcaaggcttt ggcacgacga
     4921 taggctgaga agcgttcttg ggcttggtgg cattcaatcg caaattcgac ttcaaactca
     4981 cgcaagcatt gtgcttctgg cgttgggaag tagccccagt cacctagctc acctgatgca
     5041 cgcaaaatgg tcacggcaat ggtgttatcc ccaaggattt cgtattcatt caatcccttg
     5101 ttggatacag tcaccccttt ttcatcgtca tacagactga caaaagcttg ttggtgttga
     5161 ggattttcag gattttccca tgaagcagct ggtttgtttg gtcgtgtcac cacctcatag
     5221 atactttcag aatcattgct tggacgcgtg ttatgagtct tgaccaagag acggatacgg
     5281 tgatccttgg cagtgttagt aaagcgagtc ttgaagcgga tttgtggatt gtcaacaaag
     5341 acaatcaact cagtttccag aggaatgttt gtcaattctt ctgaccgtcc agcctcacgc
     5401 ttcataaact cgatgatacc ttgttgctct tcttctagct tttcatccgc actgacaggc
     5461 acggtcaatt catgtttgag caagatttta gcatagcgat ctgtgttttc caagacctcg
     5521 tggcccttaa gctctgcaaa gattggctct gttccttttg gttggaaata gatatactcg
     5581 tttccgatgt caccccggtc ttcgaagcgg ataaaatctt cataggcttc gtgagttgtc
     5641 ttatcataga ctgtgatgtt gtcatccaca ctcaccgtta cgaatggtgt atcaatcact
     5701 ccgttttggt aaataccctc acggtgttct tgttctcctt ccagcaattg gaaggttgtc
     5761 caagaaagcg gtgctaggtg aactggaatg gtcacgcgca cttgtcgagc aatacgagct
     5821 tggcggaact tatcttttgg taaatcgtat tcaaaattag ctccgaggtc ttcgattgta
     5881 acctctacag gacgaccatc caagtcctcc acacggtaac ttggcaagat aagagcagcc
     5941 atctttttgt agccttctgt tgggtgcaat tccttgaaat cacaagtcgc cacatcaatc
     6001 actgtgctga cagtatcgac cttatcatgc aagcctgtgt taatgacagt aaagagatag
     6061 tcactttgag ccttatccgt agcaatttta cccttccact cgttgagcaa attactttta
     6121 acaaagtttc ctacttggtt gaccttggca aaacgcgttt ccatctcgcg gtgaacttcg
     6181 tccacgctac agccacagat actatcatgt ggcgcattct gcaaaagtgt tttccaagca
     6241 taggtcaact ggtccttgtg gttgtgtcca ccagtgataa tagtcaaggg ttctaccact
     6301 tgctctagga ggttgctatt ttcttggaag gcttgtttta ggtaaatgcg ggatgaagaa
     6361 gtgttggcaa gtgtgtacca gccatctgtt tcctgactgg tcaactcgcc tgtaacagtt
     6421 gataagtgtt caggaagcgc accttctaca gcttgaacat attcatcaaa agaactatga
     6481 acaaagatta catccgggaa gagttcattt gccacacgaa tggcttcgct cagatttttc
     6541 tgtacaggct ggtggtcaca gccgttcatc atcaaccatt ggttggtcga agcgtaggca
     6601 cgcacatctg acaatttttg tttccagaag gtcaaggcct catctttgtc aactgggatt
     6661 tcattcccat tactgtacca gttggcaaag agaataccta aaacacgact accatccaca
     6721 ccctgccagt acatttctga aaactgggat gtaaactgct catcttcaag gacttggttg
     6781 tcaaatccaa tcggcttcac accacgacca aaggccgcca cgtgaatgcc tgatttttga
     6841 agaatttgag gcgcttgtcc catatttcca aaggtatctg gaaagtagcc aatctgggtt
     6901 gatttacccc atttggcagc ttcttgttga ccaatcaagg tattgcggac attggcttca
     6961 ctggagatca agtagtcatc ctgcaagatg taaaagggac caattttaag tttgccctcg
     7021 tcaatgtagc gttggacctt gtcgcgattt tcagggcgaa tttgtaagta gtcatcaagg
     7081 acaatagttt gtccatccaa gtggaaactc ttgaactcag ggtcattttc aaagagatca
     7141 aagagattgt caaacaattc caccaactgc atacgatggc tttcaaaagg caagtaccac
     7201 tcacggtccc agtgactatg tgagataata tgtacaacaa cattttccat gaagtaaaac
     7261 ctcattctaa atttaaattt taacgtttta aatgtaaact tgtgattctg ataagaatcg
     7321 gttgagctaa agcgagcccc ttagcgaata tccaagtaat ccaagaccaa ctcacagaac
     7381 atcatgttag cccaggagaa ccattcacga gagtagaggg tcggatcatc tacgtggaag
     7441 ctttcgtgca tgacacctgt accaccatcg caggcaacca gctgatccag caagaatttt
     7501 ttctctgcct tatctcttgt tgtcaatcct tggatagaaa gggcgattgg ccagatatag
     7561 cgatagaagg tatgagaact tccgagaccg ctagcgtatt ctccttggta gaagtatgga
     7621 ttttcagagc tcagaatggt acggcgagtg gcttgataca cttcatcatc gatcgaacag
     7681 tagcccagat agggcgcagc tagtagactt ggtacatttg gatcatccat gatgctggca
     7741 tttcctaggc catctacttc aaaagcgtaa atcttttcgc ccttgctgtt ggtggtgtaa
     7801 gcgtagtttt cgattccttc ttggatttca tcctgaagac gcttggcatc agcaataaca
     7861 ctctggctat cagctaggtt taatgctgcg aagatttctt gcacataacc caagactact
     7921 acagcaaaca tatttgacgg aatcaagtaa ctatactggc aacagtcatc actcggacga
     7981 aaggctgacc aagtcatacc tgtcactgca aagtcaggtc caaagccatc atttaccaag
     8041 gtgtcttcct tacggtccgt atctcggaca aaacgataag gagagttctt gtggtcttgt
     8101 tcaactgtcc atagatggag aatttcctta gtcgctgcga caaaagtctc atcaaactga
     8161 ctagtctcgc cagtttcttt ccagaggaga taagccaact gcaaaggata gcaaagcgag
     8221 tccacctcat acttgcgttc ccaaatccag ccattaaggt cggtgtggtc ggtctcgtgg
     8281 tgccccttcc agttttcctc tatgttgaag gaattggcat aaggatcctt gagtaccaag
     8341 gtcatctgac gtttgaccaa acctgcaatg gtctgacgca ggagggcatc tcttttagct
     8401 acatgcaggt agggtctgag ttgggctgtc gaatcccgaa gccacatagc aggaatatcc
     8461 ccagtcaaga caaaagttga accatcttct aagatttcaa ccgtattgtc caaggtatcg
     8521 gtgtagcaac gctcgaaaac atccacccac tctggatggt ccttagcccg ctctgctact
     8581 tcatctagcc attctctaac aatttctttc ggataaatca tcgtgcaatt ccctctttca
     8641 aacaagtttc atgttcagta tatagctttt gagacagaaa atacatacac aaatgatagt
     8701 catttttcta caaaatagtt atttttgaaa ctccttttct aaaggccctc tttttctgat
     8761 ataatgtaga aaaacagcta aaggaaagac tatgaaacca ctacttgaaa ccatcgatac
     8821 ccgctttggt actgctagca agcatgcctt ctcttgggga aataccctgc catacacagg
     8881 cgtgcctttt gggatgaatt actttgtgcc ccagaccagt gaccaggatg gatcttggtt
     8941 ttttgatccg catctgccta tctttcaggg gattcgatta actcaccagc ccagtccttg
     9001 gattggggac tactcttggc tccttctgac acctgtcacc agtcaactgg ggggagacag
     9061 cctctttcac cgccagtctt cttatgatat agataaagct tgcttccagc ctcactattt
     9121 gaagctcttt tctctgcgtt atcagattga aacccagctc acaccaactt gctatggtgc
     9181 ttctattcgt ttgaatcaaa agcaaggcaa agccctctcc ctctatcttc acgcagcaga
     9241 tgaactgaca gtagagcaag tagataagcg gactcttgcc ctgcgacaag aaggtaaaac
     9301 tgaaactaac aaaaattcgc taacgatgtt cactgccctg caaatgaaca cggatattct
     9361 tgctatcagc caagaagctg gagactggcg aattgactta gtaagtagtc aaaccgagat
     9421 gcaactagcc acttctttca tctctccttc tcaagctcta atcaatctac ctcaagaaga
     9481 ttttgatagc tgtaaatcaa gtgcccaagc ggattgggaa aatctcctcc atcgttttga
     9541 cattatagag acaggagagg ctgaccgaac cttctttgac cactgcctct acagactctt
     9601 cctcttccca cagacttttt atgaggttga tgagtcaggg caagccatcc acatggatct
     9661 ggctactggt actgtcaagc ctggtgtcct ctttagcaac aatggtttct gggatacctt
     9721 ccgcaccacc ttccccctct ttgcccttat cataccggag cactatcaac gctttttaga
     9781 aggtttcctc aatagctacc gcgatactgg tttccttcca aaatggctgg ctccagatga
     9841 acggggtatg atgccaggta cacttttaga cggtattatc gcagatagcg cctgcaagga
     9901 catggcccct gacctagaag gagaactctt ccaagccatg ctcgaaacag ccagcaaagc
     9961 cgaccctctc ggcatcaatg gccgccacgg actagcccaa taccaagaac taggttacct
    10021 ctctaccgac caccatgaaa gtgttagcca tactctagac tatgcctata gtgacttttg
    10081 tatcgccagc tgtgccaaaa aactagagaa catagaaatc gctgaaacct acaaggctgc
    10141 ttcacaaaat taccgccagc tatttgacgc tgagacaggt tacatgcgag cacgagacaa
    10201 tcaaggaaac tttcaccctg acttctctcc ttatagttgg gggcgagact acgctgaatg
    10261 ctctgccatt caagctactt taggcgttct ccacgacatc cctggcttaa tccaactgat
    10321 gggtggaaaa gaaaccttta gcaactatct tttgaaagcc tgtcaagatg ctcccctctt
    10381 tgaaacaaca ggctatggtt acgaaattca cgaaatgagc gagatggcta ctgctccttt
    10441 tggacaaata gccatttcca accagcctag tttccacatt ccttatctct tccgctacag
    10501 cgattaccct gactacactg cccttcttat caagacgctc cgtcagaaag cttttcaccc
    10561 aagttgggaa gcctatcctg gagatgaaga caatggtagt ctctctgctt ggtacatctg
    10621 gtcagctctc ggattttatc caacctgtcc aggaaaacca agctatgacc tcggaatccc
    10681 tctctttgac catctacgag tctacctagc taaagaagat aaatggctgg atatccatac
    10741 taaacaaaac cacaaccatt ttaactttgt caaagaatgc cgactggaca aaatactcgt
    10801 atcaactatt caacaccaag acctcttaaa agctgaacaa ctaactttca ccctcagctg
    10861 gttaccaagt cactaataac caaaagaacc tttgcatcgc gcaaaggttc ttcttttatg
    10921 aaacttggac ttgaagttga tccacaagct gaccatccac cgttaggttc agatagaaat
    10981 acaaggtttt atctcctgcg aaatacaagg ttggtagcgt cagacttttt tcagtctcac
    11041 cagcttgaaa cgcaaggact tgaatctcat cctgataggc gacaccatgg acacctgtcc
    11101 ccggttgaat cgaaatctta gcttctaaag gaccactaca ttctctgcgc ttaactgtaa
    11161 aataggcatt ttctcccttt gccacagcta ggcttttttc tgcaaatgct agttcctgaa
    11221 caacttcttt ttttgataat ccaggagttt tataaaggga aatcttggtc aatacaggaa
    11281 tagattgtgc ctcggtaatc atcacgcgca ccttctgtgc ctctactagc ggacctcgca
    11341 agaggcgctt atgaccaact gtgaagcccc tgccaaattc ctgccagaca ccatccacct
    11401 ctacttgcac atgaaaagca gcgattcgtt gccctagctt caaatcttct cttaactcaa
    11461 ttacatcaaa agttttagga gaacctaagt cgagttctaa ctggatgggc aagtctgcat
    11521 cgtttgccca agagctggtc tcaaggccgt ctgtcaaatg gcgacaagca aagtctgcgg
    11581 aaagagctgg accagatacc tcagctccca gagccaaatc ttctttatag agctcattgc
    11641 gataggtcgc aaattcataa agtcgttcaa tatcctttgc atcaaagagc ccagcttgat
    11701 tcggcggaat attaagcaag agtggagttc ctcgccctac tgagtgaaag tagatttcga
    11761 ccaactcctc gagagactta ggatcctgat cctcatggta gaaccagcct ggacggatgg
    11821 aaacatctgc ctctccgatt gaaaaaatcg tgcccgaggg atccccgtgc tgaagatagt
    11881 ccagctctgc ttctgttcct agtttatcag gattcacctt ttgccacagt ggatcacctg
    11941 cataccctcg ttcattgcca atccagcgga tactggtgcc ttctgttgaa aaaatcaaac
    12001 aatcgccctg caagtcacga atggtttcaa accatttttc aaattcataa ttgacctgct
    12061 gggccccttc ccctctagca ccatccatcc aaacctcagc gaacttacca gcattcccat
    12121 agttaggatt tgataagatt tccttcaact gagccagata ataggcattg tagtccgctt
    12181 ctcggtccac atgatagagg ggactatggg catcccacgg tgacaggtag acccccatat
    12241 ccatatcaaa ctctgtggca gcttgggata cttcaaggag caagtcgccc tttcctctcc
    12301 tccaaggact gaccttaacc gaataatctg tgtgagctgt cggataaagg acaaagccat
    12361 cgtggtgctt gaccaccaaa atcaactttt tgaagcccgt ttccttgagc acacgaaccc
    12421 actcacgcgc atccaactga ctcgggttaa agcgctcagg atcctcctga cctgttcccc
    12481 attcctgatt ataaaaagta ttaggtccaa aatggataaa ggctgctagt tcatctccca
    12541 gataagctag ctgagtctga cttggtaacg gtccatgcgg tttgattttc ttcatcgtta
    12601 tatatctcct tcttttgcaa cttctcttgt tcacaaggtt atccacaggt tgtggataat
    12661 aaaccatgat ctaacagcca gctctttcca actccaactg gtaggctcag gatttttctc
    12721 ctcaatattc cctcttcaaa cagctctgtc atatggttct gctaacacct ctcagaatgc
    12781 tactctccat aattattctc aggcttcttt caggtcattg tgtgaagacc attcactctc
    12841 cagacttttc cacagattgt gaataagtga ttattctaaa ctatcacctc gtcgactata
    12901 acaaaaaata aaaagagggt caataagata attatctctt cctaaatact cagaaaagat
    12961 tctataattt gtacaattga acaagaaatg cttctccaaa atctatcttc aaaagaatgt
    13021 tgcatctttt tatcctttca cacaaacatt tctctttata cagatccaag tgataaccag
    13081 atgataattg gacacttttt ggttatttgt gtattttttg aaaaattttt taaaaaaatt
    13141 t
//
LOCUS       DAVLVF010000040        13105 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVLVF010000040 DAVLVF010000000
VERSION     DAVLVF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13105
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.040"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            59..181
                     /locus_tag="VYN66_001223"
     CDS             59..181
                     /locus_tag="VYN66_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine sulfhydrylase"
                     /protein_id="HEV1495245.1"
                     /translation="MTCDFKSETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
     gene            203..1243
                     /locus_tag="VYN66_001224"
     CDS             203..1243
                     /locus_tag="VYN66_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEV1495246.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWMASGKFPQFVDEGSSCHNLSYTRDVGAVAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKDVGSIFTFHVKGGEEEARKVIDHLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            1414..2688
                     /locus_tag="VYN66_001225"
     CDS             1414..2688
                     /locus_tag="VYN66_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV1495247.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELCLASVKQNYEAQL
                     KAASEQVEFYKDFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            2698..3576
                     /gene="truB"
                     /locus_tag="VYN66_001226"
     CDS             2698..3576
                     /gene="truB"
                     /locus_tag="VYN66_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV1495248.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALSLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            3817..4119
                     /locus_tag="VYN66_001227"
     CDS             3817..4119
                     /locus_tag="VYN66_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1495249.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            4227..4865
                     /gene="udk"
                     /locus_tag="VYN66_001228"
     CDS             4227..4865
                     /gene="udk"
                     /locus_tag="VYN66_001228"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV1495250.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            4993..6333
                     /locus_tag="VYN66_001229"
     CDS             4993..6333
                     /locus_tag="VYN66_001229"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV1495251.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            6311..6523
                     /locus_tag="VYN66_001230"
     CDS             6311..6523
                     /locus_tag="VYN66_001230"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV1495252.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            6520..7395
                     /locus_tag="VYN66_001231"
     CDS             6520..7395
                     /locus_tag="VYN66_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV1495253.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQIIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            7388..8203
                     /locus_tag="VYN66_001232"
     CDS             7388..8203
                     /locus_tag="VYN66_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV1495254.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQIFAEIKEAVERAHSQFKNE"
     gene            8196..8627
                     /locus_tag="VYN66_001233"
     CDS             8196..8627
                     /locus_tag="VYN66_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1495255.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            8634..10301
                     /gene="recN"
                     /locus_tag="VYN66_001234"
     CDS             8634..10301
                     /gene="recN"
                     /locus_tag="VYN66_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV1495256.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKERIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVHFSKGKFSRGGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            10303..11031
                     /locus_tag="VYN66_001235"
     CDS             10303..11031
                     /locus_tag="VYN66_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV1495257.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            11110..12933
                     /gene="lepA"
                     /locus_tag="VYN66_001236"
     CDS             11110..12933
                     /gene="lepA"
                     /locus_tag="VYN66_001236"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV1495258.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPESVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         3897 a         2399 c         3149 g         3660 t
ORIGIN      
        1 gtaaactata actcaaggtg ctacaatatc cttaataaaa tgatatggag gtcaccttat
       61 gacttgtgat tttaaatctg aaactctaca actacatgct ggtcaagttg tggctccagc
      121 tactaagtct cgtgcagtgc cgatttatca aacaacattt tttgtttttg atgacacgta
      181 ggaaggtgcc gatctgtttg ccttgaggaa accagggaac atttatactc gtatcaccaa
      241 tcctacaaca gctgcccttg aaggtggtgt tgaagcgcta gcaacagcat caggtatgac
      301 tgcagtgact tatacgattt tggcgcttgc ccatgctggt gaccatgtag tggctgcttc
      361 gactatttac ggtggaacct tcaatctttt gaaagaaccc cttcctcgtt atggtatcac
      421 aacaaccttt gtcgatgttg ataatttgga ggaagtagaa gcagctatca aagacaatac
      481 caagcttgtc ttgattgaaa ccttgggtaa ctccttgatt aatattccag acctggaaaa
      541 actggcagag attgctcata aacatcaaat cccacttgtg tcagacaata cttttgcaat
      601 accttatttg attaacgtct tctctcatgg cgttgacatt gccattcact ctgcgactaa
      661 gtttatcggt gggcatggta caactattgg aggaataatt gtcgatagtg gtcgttttga
      721 ctggatggct tcagggaaat tccctcaatt tgttgacgag ggttcaagct gccacaattt
      781 gagctatact cgtgatgtgg gtgcagtagc ctttattata gctgttcgag ttcaattgct
      841 tcgtgataca ggtgcagcct tgtcaccatt caatgctttc ctcttgctac aaagacttga
      901 aacctcttca cttcgtgtgg aacgccatgt acaaaatgct gagacaattg ttgattttct
      961 tgtcaaccat cctaaggtag aaaaggtaaa ttatccaaaa cttgcagata gtccttatca
     1021 tgccttggct gagaaatact tgccaaaaga tgtcggttca atctttacct tccatgtcaa
     1081 aggtggcgag gaagaagcac gcaaggtcat tgatcattta gaaatctttt ctgaccttgc
     1141 aaacgcggca gatgctaaat cgcttgttgt ccatccagca acaaccactc acggtcaatt
     1201 gtcagaaaaa gacctagaag cagcaggtgt cacaccaaac taaattcgtt tgtcaatcgg
     1261 tcttgaaaat gtagaagatt tgattgaaga cttgcgcttg gccttggaaa aaatttaaag
     1321 taaaagaaga taaacagtgg gcttcgactc actgtttttg attttccctc aggcatgata
     1381 taatggttaa agaagtctag aaagaggaac gatatgaacg aaatcaaatg tcccaactgt
     1441 ggggaagtct ttacagtaaa tgagagtcag tatgccgaac tcttgtccca agtgagaacg
     1501 gcagagtttg ataaggaact acacgatagg atgaagcagg aactggcctt ggctgagcaa
     1561 aaggccatga atgagcaaca gactaaactg gctcagaagg atcaagaaat tgcgcaatta
     1621 cagagtcaga tccaaaactt tgatacagaa aaagaattgg ccaagaaaga ggttgaacag
     1681 acaagccatg aggctctctt ggctaaggac aaggaagtac agctcttaga aaatcagttg
     1741 gctaccttgc gtttggagca tgaaaatcaa ctgcaaaaga ccctttctga cctagaaaaa
     1801 gaacgggatc aggttaaaaa ccaactactt ttgcaggaaa aggaaaatga attatgtttg
     1861 gcttctgtta agcaaaacta cgaagcccag ctcaaggcag ctagtgaaca agtcgagttt
     1921 tataaggatt ttaaggctca acaatctaca aaagcgattg gggaaagcct agaacagtat
     1981 gcagagagtg agtttaacaa ggttcgtagt ttcgcctttc caaatgctta ctttgagaag
     2041 gataacaagg tctcttcgcg tgggtctaaa ggggacttta tcttccgtga gtgtgatgaa
     2101 aatggagttg aaatcatttc tatcatgttt gagatgaaaa acgaagcaga cggaacagag
     2161 aagaagcaca agaatgcaga tttttacaag gaattggaca aggaccgtcg ggagaagaac
     2221 tgtgagtatg ccgttttggt gaccatgctt gaggccgata atgactactt taacacaggg
     2281 attgttgacg tcagccacga gtatgaaaaa atgtatgttg ttcgtcctca attctttatc
     2341 cagttgattg gtctcttacg taatgcggcg ctaaattccc taaaatacaa gcaggagttg
     2401 gccttggttc gcgagcaaaa tattgacatt acgcattttg aggaagactt ggatgccttt
     2461 aagctagctt ttgctaagaa ctataattca gcttcgacta actttggaaa agctattgat
     2521 gaaatcgaca aggccatcaa acgcatggaa gaggttaaga aattcctgac tacatctgaa
     2581 aaccaactcc gtctagctaa caacaaattg gaagatgtct ctgttaaaaa attgacccgg
     2641 aaaaatccaa caatgaaagc gaagttcgaa gcactgaagg gggagtagaa agcaaaaatg
     2701 aacggtatta tcaacttaaa aaaagaagca ggaatgacct cgcatgatgc ggtttttaaa
     2761 ctgcgtaaga ttttgggaac caagaaaatt ggtcatggtg gaaccttgga tccggatgtg
     2821 gtgggtgttt tgccgattgc ggttggcaag gcgacacgca tggtcgagtt tatgcaggac
     2881 gagggtaaga tctatgaggg ggaaattact ctgggttatt ccacgacgac tgaggatgct
     2941 agtggggaag tgatcgcaga aacccctgtt ttgtctccct tggatgaaaa gcttgttgat
     3001 gaagcgattg ctagcttgac tgggcctatt acccagattc cccctatgta ttcggcagtt
     3061 aaggttaatg gtcgcaagct ctatgagtat gcgcgtgctg gtcaggaagt ggagcgtcca
     3121 gaacgtcagg tgatcattta tcaatttgag cgaacaagtc cgatttctta tgatggccaa
     3181 cttgcccgat tcacttttcg tgtaaaatgc agtaaaggga cttacatccg tactttgtca
     3241 gttgatttgg gtgaaaagct tggttatgcg gctcatatgt cccatttgac tcgtactagt
     3301 gctgctggct tacaattaga agacgctctt tccttggagg aaattgctga aaaagtagag
     3361 gctgggcaat tagattttct ccatccttta gagattggga caggtgacct tgtcaaagtt
     3421 ttcctaactc cagaagaggc tacagaagtt cgctttggtc gttttattga gctagaccaa
     3481 acggacaaag aactggctgc ctttgaagat gataaattgt tagccattct agaaaaacgg
     3541 ggcaatctct ataagccaag gaaggttttt agctagatcg tttaggaata aaaatcgggt
     3601 gatagataac aattgcttga taaaacccca tactaatagt agaatggttt tgggaattat
     3661 aatattccaa ttgttgcgag ttgtaggtac tcaaataatc tatatagaaa tttagaggtg
     3721 tgaaatgaag caatttaaaa ttctttcaga taaatattta gagtccatta caggttctgg
     3781 tgggaactta ggcccaggat ttggtgtgat aattccatga tgcgaaatga gtttcgagaa
     3841 agggtggagc aacttcttca acaaaaagaa ataaatgaaa atagtgagtt gagtcacctg
     3901 tttcgtcttg ctatacaaaa tttagacaga aatgaaaaat accaatcggt catggccaat
     3961 ttgagtcaag ggttgtcact ttacctcatg acgcatcatt accaggcacc taagtctgtc
     4021 attgattttg gtttatggat tgccaaagct cctagccagg aaagagggag actggctttc
     4081 ttgcagatgc ttgctcaaac cttacagggc tttcgttaaa actgtcgaac atcagaggga
     4141 agactctggt gttttttgct gatgagacta taaattttgt aaaaaatgtg atagaataga
     4201 cgacggataa aataacggag gatagcatgc aaaatagacc aatcattatt ggagtgacag
     4261 gtggttctgg tggtggtaaa accagtgttt caagagccat tttatcgcat tttcctgatg
     4321 aaaagatttc catgattgag catgattcat actacaagga tcagtctcat ttgacctttg
     4381 aagagcgtgt caaaaccaac tatgaccatc cttttgcctt tgatacagac ttgatgatcg
     4441 agcagattaa ggaattgttg gcagggcgtc cggtggacat cccgacctac gactatacag
     4501 agcatacacg gagtagcaag acctatcgtc aggagcctca agatgtcttt atcgttgagg
     4561 gcattttggt cttggaggac aagcgtctgc gcgatttgat ggatatcaag atttttgtgg
     4621 atacggatga cgatgtgcgt attattcgtc gtatcaagcg tgatatggag gaacgtggcc
     4681 gtagccttga tagcgttatt aaccagtact taggtgtggt caaaccaatg taccaccagt
     4741 ttatcgagtc aactaagcgt tatgctgata tcgtcattcc tgaaggggtt agcaataccg
     4801 tggctatcga cctgttgacg accaagattg caaagatttt ggaagaagct cgaaacagca
     4861 aataatcaca tgtggaggcc ttgcctcctt tttctatttt tccctcataa tggtacataa
     4921 atggagattt ttaggcatat ttttggtata ataataccca taaaagagca agtaaatgaa
     4981 tagtaggtgg agatggaaaa gtatttatcg gtaacaactt tgaccaaata tctgaaaatg
     5041 aaattcgata aagacccata cttggaacgg gtctatttaa ctggtcaagt ttccaacttt
     5101 cgtaaacgac ctactcacca atatttctcc ctaaaggatg accatgcagt tattcaagcg
     5161 accatctggt ctgggattta tcagaaatta gggtttgacc tggaagaagg aatgaagatc
     5221 aatgtgattg ggcgtgtaca ggtctatgaa ccaagtggta gctactccat catcattgaa
     5281 aaagctgagc ctgatggggt tggggcgctt gcgattcagt ttgaacaact taagaaaaaa
     5341 ttgacagaag aaggcctgtt tcaagaacgc ttcaagcaag ctctacccca attttctaag
     5401 agaattgggg tagtaaccag ccgtagtgga gccgttattc gagatattat cacgaccgtc
     5461 agcaggcgat tcccaggtgt tgacatcctt ctttatccta ccaaggttca aggtgaaggg
     5521 gctgcggagg aaattgctag aaatattgcg cgtgctaatc aacgggacga tttggatttg
     5581 ctcattattg gtcgtggagg tggttctatt gaggatctct gggcctttaa cgaagaaatt
     5641 gtggtacgag ctatttttga atctcgtttg ccagttatct ctagtgtggg gcatgagacg
     5701 gatgtgacct tggcagattt tgtggcagat cgacgcgctg caacgccaac agcggcggct
     5761 gaactggcaa cacctgtgac caagttggat gtattaactc atttgcaaaa tcaggaaaaa
     5821 cggatggcaa cggcagtccg aaatgttcta tctaagaaac aagaggcttt gaaaaaatgc
     5881 agtcagtctg ttatctttag acaacctgag cgcttgtatg acggttattt gcaacgcttg
     5941 gaccaactgc aactgcgttt gaaacaaagt ttgcgaactc ggatttctga taacaaacaa
     6001 ttagttcaag caagaactca tcaattagta caattatcac ctgttaccaa aatccaacgc
     6061 tatcaagacc gtttaggaca gttggacaag ctcttaggta gccaaatggc gttagtttat
     6121 gacgccaagg ttgctgaggc caagcgactt tcggaagctt tgctcatgtt ggatactagc
     6181 cgaatcgtgg cgcgtggtta tgctattgtc aaaaaagaag agtccattgt agattcggtt
     6241 gagagtttga agaaaaaaga ccaagtaacg cttttgatgc gagatggtca agtagaatta
     6301 gaggttaaag atgtcaaaac aaaagaaatt tgaggaaaat ctagcagaac tggaaaccat
     6361 tgtccaaagt ttggaaaatg gtgaaattgc tctggaagat gcgattactg cctttcaaaa
     6421 gggcatggtc ttgtcaaaag agctccaagc tacgctggac aaggctgaaa agaccttggt
     6481 caaggtcatg caagaagacg gaacagaaag tgattttgaa tgaaaaagca agaaaaatta
     6541 gctcttgtcg agtcggcttt ggaagatttt tatggagacc agcagtttgc ctctagttta
     6601 cgggagtctg ttctctattc tattcatgct ggtggcaagc gtattcggcc ttttctcttg
     6661 ttagaagttc tggaagcctt gcaggttacc atcaaacctg ctcacgcgca ggtagctact
     6721 gccttggaga tgattcatac agggagcttg attcacgatg accttcctgc tatggatgat
     6781 gacgattatc gaagagggcg gttaaccaat cacaagaaat tcggtgaagc tatggccatt
     6841 ttggctggag atgccttatt cttagaccca tatgccttga ttgcgcaggc agatttgcca
     6901 agtcagatta aggtggactt gattgccaac ttatcccttg cttcaggtag tctgggtatg
     6961 gtggcagggc aagttttgga tatggagggc gaacaccagc acttgtcttt ggaagaactt
     7021 cagattattc atgccaataa gactgggaag ttactagcct atcccttcca agcggcagct
     7081 attatagctg aattgtcacc tgaaatgcag gtgaagctga aaactgtggg tgaattgatt
     7141 ggacttgctt ttcaagtcag agatgatgta ctggatgtga cagctagttt tgaggaaatc
     7201 ggcaagacac ctcaaaagga tctgcaggca gaaaaatcaa cctatcctgc cctgttgggc
     7261 ttggaagagt ccattgcctt ttgtaaccaa accctggatc aagctaatga aaaattagaa
     7321 gaaattgccc agcagcttcc ctttgaaaca gaatcgattg taagtgtagt agaaagtttg
     7381 agaatcaatg gctaaggaaa gagtggatgt actagcttat aaacaggggt tgtttgaaac
     7441 gagagagcag gccaagcgag gtgtaatggc tggcctagtc gtagcagtcc ttaatggaga
     7501 acggtttgac aagccaggag agaaaattcc agatgacacc gaattaaaac tcaagggaga
     7561 gaaactcaag tatgtcagcc gtggtggttt gaaactggaa aaggccttgc aggtctttga
     7621 tttgtcggtg gatggcgcga ctacgattga tatcggggcc tctactggag gttttaccga
     7681 tgtcatgcta cagaatagtg ccaagttggt ctttgcagtc gatgttggta ccaatcagtt
     7741 ggcttggaaa ttacgccaag acccacgagt tgttagcatg gagcagttca atttccgcta
     7801 tgctgaaaag actgatttcg agcaggagcc gagctttgcc agtattgatg tgagtttcat
     7861 ttcccttagt ctgattttgc cagccttgca tcgtgtcttg gctgatcaag gtcaggtggt
     7921 agcacttgtc aaacctcagt ttgaggcagg acgtgagcag attgggaaaa atggaattat
     7981 tcgagatgct aagattcatc agaatgtcct tgaatctgta acagctatgg cagtagaggc
     8041 aggtttttca gtccttggct tggacttttc tcccatccaa ggtggacatg gaaatattga
     8101 atttttagcg tatttgaaaa aagaaaagtc agcaagcaat cagatttttg ctgagattaa
     8161 agaagcagta gagagggcgc atagtcaatt taaaaatgaa taaaaaagag agacttgaaa
     8221 aaattagaag attggtgaca gattatcaaa tcggcacgca agaagaaatt gtagaacatt
     8281 tgaaagaagc aggtatcact gccactcagg cgacggtatc ccgagatatc aaagagttag
     8341 gtattgtcaa aattcctttg agagacaaca cctatgtcta cgaattacca aaatcaatcg
     8401 taaaaagtct gcaactggct gaagacaata tcgaatcggc tgaattgatg gataagatga
     8461 tcaatctcca agttattcca ggaaatacgg cttttgtaaa agctcagtta atcgatactt
     8521 ttgcagacaa gatttttagt tgtttgactg atgatagctc gattttagtc attgccagaa
     8581 gtggaagtct agcagaggaa atctttgaac aagtaaaaaa ttggtaggtc tatatgttac
     8641 ttgaaatttc gataaaaaac tttgccatta ttgaggctat ttccctcaat tttgaaaagg
     8701 ggatgactgt cctgactggt gaaacgggtg cagggaagtc gattatcatt gatgccatga
     8761 atatgatgtt gggagctcgt gcgacgacag atgttattcg tcatggtgca ccaaaggcag
     8821 agattgaggg gcttttctca gttgagaata gtcgtctttt acaggaaatt tttgatgagc
     8881 aaggtttaga attaggtgat gaaattatca tccgtcggga aatcttgcaa aatggtcgta
     8941 gtatcagccg tgtaaatggt cagatggtta atctgtctgt tttgcgagct attgggcaac
     9001 atcttgtaga tattcatggt cagcatgacc atgaggagtt aatgcgtccc caactgcata
     9061 tccagatgtt ggatgaattt ggtgatgccg ctttttggga cttgaaagaa acctatcaaa
     9121 cgagttttga tgcctatcgg aaaatgcgca agcaggttct ggaagtcaag aaaaaccaac
     9181 aggaacacaa ggaacgtatc gaaatgttgg aatttcaaat ggcagagatt gaggcagcaa
     9241 acttgcaggc tggagaagac ttggctctca atcaagagcg agataaactc ctcaaccaca
     9301 aaaatattgc ggatacactg accaatgcct acagtatgtt ggacaatgaa gatttttcaa
     9361 gtctggccaa tgttcgttca gctatgaatg acatggaaag tgtcgaagag tatgatcctg
     9421 aataccgtga aatttcaagc tctctgtctg agacctacta tgttttagaa gacattagca
     9481 aacgtttgga agctatcatt gaggaccttg attttgatgg caatcgcctg atgcaggttg
     9541 agaatcgttt ggacctcctt catactatta ctcgtaagta tggtgggact gttgatgatg
     9601 ttttgcttta ttttgccaag attacggaag aatacaatct cttgacaggc aataatcttt
     9661 cgtctgagga catggaagca gagcttaaga agttggaagt caatcttgtc aatttggcag
     9721 gtcaacttgc ttctgctcgt cataatttgg ctcagcaact cgaagctgag attaaacaag
     9781 aactgcaaga tctttatatg gaaaaagccc agtttcaggt tcattttagt aagggaaaat
     9841 tcagtcgtgg gggaaatgaa atggttgagt tttatatttc aaccaaccct ggagaagact
     9901 ttaaaccctt ggttaaggtt gcttctggag gggaattatc tcgtctcatg ttagccatta
     9961 agtctgcctt ttcacgtaaa gaaggcaaga ctagcattgt ctttgatgag gtggatacgg
    10021 gagtttcagg tcgtgttgct caagctattg ctcagaaaat tcataaaatt ggtcagcatg
    10081 gtcaggtttt ggctatctcc catttgccac aagtaattgc gattgcagat tatcaattct
    10141 ttattgagaa gattagtaat gaccattcaa cggtttcgac tgttcgtctc ttgacagtcg
    10201 aagagcgagt ggaggaagtt gccaagatgt tggcaggtga tgatgtgaca gaagcagccc
    10261 tgacgcaagc cagagaattg ttgagaaaca gggagaaata agatgacaga ttattatgta
    10321 attggagatg ttcacggaaa agctgggatg ctggaagacc ttcttaaaac ctgggatggt
    10381 cagacccagt tgctctttct aggtgatttg attgacagag gtgaggatag tcaccgtgtt
    10441 cttgaaatgg tcaaggactt ggtggacaat caaggggcta tctgtttgtc tggaaaccac
    10501 gagtatatgt ttttgacttg gctagatgac ccagaagaaa gttatgacca ttatcgtcgc
    10561 aatggtggag atacaaccat taactctatc ctaggtcgtc ccttggatgc accagttgat
    10621 ggagtagaag atgccaagcg ggttgccgct gaagcgtcag acttggtcga attcattcgt
    10681 caaatgccat ttgtagtaga gacagacaag tatatatttg ttcacgcagg tattgatttg
    10741 actttggacg actggcatga aacaacagat tataaaaaag tatggcttag aaaaccattc
    10801 cacggagctg aaaatcatac tggaaaaacc attgtcttcg gtcatacacc ggtctatggt
    10861 ttgttacagc aagaacgcgg tacatctgag ctttggacta cagaagatgg caagattgga
    10921 atggatggag gagctgttta tggtggtgtc cttcacggga ttgtctttac agaccaagga
    10981 atgacagaac accactttat cgaaaatgac ggctttgtcg ctgaagatta gtactcctag
    11041 cagggtatgg tcttgtcaaa atatcaaaaa caatttataa taaatagata ccctgaaagg
    11101 aagagaatca tgaacttaga agaattgaaa aaacgacagg ggaagatccg taacttctct
    11161 attatcgccc atattgacca cgggaaatca actctagcag accgcatttt ggaaaaaaca
    11221 gagacggttt caagtcgtga aatgcaggcc cagcttttgg atagcatgga tctagagcgg
    11281 gaacgtggaa ttactattaa gttgaatgcc atcgagttga attacactgc aaaagatggg
    11341 gaaacttata ttttccactt gattgacaca ccagggcacg ttgactttac ctatgaagtt
    11401 tcacgttcgc tagctgcctg tgagggtgct attttggtgg tcgatgcggc tcaaggaatt
    11461 gaggctcaaa ctcttgccaa cgtttatctg gctttggata atgatttgga aatcatgcct
    11521 atcattaata aaattgacct gccggctgca gatccggaga gcgtgcgtac agagattgaa
    11581 gatgtgattg gtttggatgc cagtgaagca gttttggctt ctgccaaggc tggtattggg
    11641 attgaagaaa tcctcgagca aattgtagaa aaagtaccag caccaacggg tgatgtgacg
    11701 gcgccactta aggccttgat tttcgactct gtttacgatg cttaccgtgg ggttatcctc
    11761 caagtgcgtg tcatggacgg agtggtcaaa cctggtgata agattcagct catgagcaat
    11821 agtaagacct ttgatgtggc cgaagtcggt atttttacac caaaagcggt tggtcgtgat
    11881 ttccttgcga ctggtgatgt tggttacatt gcggcgtcta tcaagacggt tcaggatact
    11941 cgtgtgggtg ataccgttac cttggcaacc aatcctgcgg cagaaccatt acatggttat
    12001 aagcagatga atcctatggt ctttgcgggt ctctacccaa tcgaatcaaa caagtacaat
    12061 gacctgcgtg aagcgcttga aaaattgcaa ctgaatgatg ctagtcttca gtttgaacca
    12121 gaaacatctc aggcacttgg atttggtttc cgttgtggat ttcttggact tctccatatg
    12181 gatgttatcc aggaacgttt agagcgtgag ttcaatattg atctcatcat gacagctccg
    12241 tctgttattt acaaagttaa tttgaccgac ggtgagtcta tggatgtgtc taacccatct
    12301 gagtttccag acccaactaa gattgcgacc attgaagagc cttatgtcaa ggcgcaaatc
    12361 atggtaccac aggagttcgt cggagcagta atggagctag ctcagcgtaa gcgtggggac
    12421 tttgtgacta tggactatat tgatgataac cgtgtcaatg ttatctatca aattcctctt
    12481 gctgaaatcg tctttgactt ctttgataaa cttaagtctt cgacacgtgg ttatgcaagt
    12541 tttgactacg aattgtcaga atatcgccca tctaagctag tgaaaatgga tatccttctc
    12601 aacggagaca aggtggatgc cctcagcttt atcgttcata aggactttgc ttatgaacgt
    12661 gggaaactca tcgttgataa gctcaagaaa atcatccctc gccaacaatt tgaggttcca
    12721 atccaagcgg ctattggaca caagattgtc gctcgtactg atatcaaggc ccttcgtaag
    12781 aacgtacttg ctaaatgtta tggtggtgac gtttctcgta agcgtaaact ccttgaaaaa
    12841 caaaaagctg gtaagaaacg catgaaatcc atcggatcag ttgaagttcc gcaagaagcc
    12901 ttcctcagcg tcttgagtat ggatgaagaa taagttatca gaaatgcctt tccttgttca
    12961 caggaaattt atataagaat caatagtaaa gcaggcttag atggtctgct tttttagttt
    13021 ttatactctt cgaaaatctc ttcaaaccac gtcagcgtcg ccttaccgta tgtatggtta
    13081 ctgacttcgt cagtttcatc tacaa
//
LOCUS       DAVLVF010000041        12855 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVLVF010000041 DAVLVF010000000
VERSION     DAVLVF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12855
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.041"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(205..1302)
                     /locus_tag="VYN66_001237"
     CDS             complement(205..1302)
                     /locus_tag="VYN66_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV1495259.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGTEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLSYILAQARENVLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGNLSIAQQNFGRVPIRIETN"
     gene            complement(1312..2052)
                     /locus_tag="VYN66_001238"
     CDS             complement(1312..2052)
                     /locus_tag="VYN66_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1495260.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(2372..2725)
                     /gene="rsfS"
                     /locus_tag="VYN66_001239"
     CDS             complement(2372..2725)
                     /gene="rsfS"
                     /locus_tag="VYN66_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV1495261.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(2738..3238)
                     /locus_tag="VYN66_001240"
     CDS             complement(2738..3238)
                     /locus_tag="VYN66_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1495262.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(3239..3832)
                     /gene="yqeK"
                     /locus_tag="VYN66_001241"
     CDS             complement(3239..3832)
                     /gene="yqeK"
                     /locus_tag="VYN66_001241"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV1495263.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            complement(3832..4461)
                     /locus_tag="VYN66_001242"
     CDS             complement(3832..4461)
                     /locus_tag="VYN66_001242"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV1495264.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(4513..4824)
                     /gene="yhbY"
                     /locus_tag="VYN66_001243"
     CDS             complement(4513..4824)
                     /gene="yhbY"
                     /locus_tag="VYN66_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV1495265.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(5038..6144)
                     /gene="yqeH"
                     /locus_tag="VYN66_001244"
     CDS             complement(5038..6144)
                     /gene="yqeH"
                     /locus_tag="VYN66_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV1495266.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFITGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            complement(6147..6737)
                     /locus_tag="VYN66_001245"
     CDS             complement(6147..6737)
                     /locus_tag="VYN66_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV1495267.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(6689..7597)
                     /locus_tag="VYN66_001246"
     CDS             complement(6689..7597)
                     /locus_tag="VYN66_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1495268.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDKPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(7654..8214)
                     /locus_tag="VYN66_001247"
     CDS             complement(7654..8214)
                     /locus_tag="VYN66_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEV1495269.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            8353..9562
                     /gene="sstT"
                     /locus_tag="VYN66_001248"
                     /pseudo
     CDS             8353..9562
                     /gene="sstT"
                     /locus_tag="VYN66_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            9630..10583
                     /locus_tag="VYN66_001249"
     CDS             9630..10583
                     /locus_tag="VYN66_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1495270.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            10726..11106
                     /locus_tag="VYN66_001250"
     CDS             10726..11106
                     /locus_tag="VYN66_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1495271.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETVAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKILASLQKTVEESLEAGLDFLI"
     gene            11205..11426
                     /locus_tag="VYN66_001251"
     CDS             11205..11426
                     /locus_tag="VYN66_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1495272.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            11443..11757
                     /gene="trxA"
                     /locus_tag="VYN66_001252"
     CDS             11443..11757
                     /gene="trxA"
                     /locus_tag="VYN66_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1495273.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            12062..12595
                     /gene="queF"
                     /locus_tag="VYN66_001253"
     CDS             12062..12595
                     /gene="queF"
                     /locus_tag="VYN66_001253"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV1495274.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWVMQHYETVEAVTFAYGQRHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEDLA
                     EQRLFQHDLYPEKIDNR"
     gene            12645..>12855
                     /locus_tag="VYN66_001254"
     CDS             12645..>12855
                     /locus_tag="VYN66_001254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495275.1"
                     /translation="MLKAVFEVVDKTDEVSSKHCFEVADRTDKVSLKHCFEVADRTDE
                     VSLKHCFEVADRTDEVSLKHCFEVVD"
BASE COUNT         3596 a         2910 c         2544 g         3805 t
ORIGIN      
        1 tccctttgta aaagcatact catcccaaga cataatctca ggaagacgcg aaaaatcatg
       61 tttaaagtga aaatcaaaga gcaaactagg aaactagccg caggctgtac ttgagtacgg
      121 caaggcgacg ttgacgcgat ttgaatttga ttttcgaaga gtattattcg taaaaaatct
      181 caaaaagtcc acctttcggt agacttagtt tgtttctatt ctaatcggca ctcttccaaa
      241 attctgttgt gctatactta gatttcctag ttggtaaatc tggtctgctt tttgggtcat
      301 agcatcccaa ggttctttgc caattcggct gactagactg acctgacctt tcaaagactt
      361 gagatgttgc ctgccttttt cagtaaatcc aaggacatga atggcttctg gcaagacatt
      421 ttctctagcc tgcgccaaga tataggacaa gaggcgtctg acacgcgcct tggtgtaacg
      481 tttggtagtc actaactcga ccaattcttc cacagactgg gctgttttaa tagcttcttt
      541 aatgcgcaca gccatttctt gattgacctg atagatggtg gttaggtcgg gatttgacaa
      601 gatttgatag cggagcaagg gaaaatagtc ttcccagatc accttactgg cctgctcaaa
      661 tagggcaaca gaaggcataa aacgttctaa gaaatcttgg tccttctgat gttgacggag
      721 ggctgtcgcc gaggcaaagt ccacatcttt attcacagaa tggtaacctg ccccctgacg
      781 ctgaatcgga tgcagcttga tattccgtcc tgcaaccgcc ttggcatagg ccagagcaag
      841 aacatgattg ggggtattac ctgaaaaatc aagaccagca aattccttcc acatagcttg
      901 ggttttctgg ggataggaaa gggaatcagg cagattttcc acaaatttct ccatctcagt
      961 acctttctct gtgtataagt cagcgatttt ctgataatct cgaacttcct ctgtcccaaa
     1021 aactagacta tcaatgccca agcgatccaa gatatccaca gctccttgac caaagaaatc
     1081 agctgcctga acactgacta aaaagggcaa ttccactacc aagtctgcgc cattttccag
     1141 cgccatctgg gcccttgtcc acttatccac aatagcaggc tctccacgct gcatgaaatt
     1201 ccctgacatg gcaacaattt tcagtccctc tgcctgatcc agcaggtatt tatggccatt
     1261 atgaaaagga ttgaactctg cgataatacc tgtgatggtc atgatgttct cctacttctg
     1321 cgccacaaaa aaccaacggg tgctagtttc tgttggctcc ttatcctcaa agtctgcata
     1381 gagtttgaag gacttaaatc cagcctgttc cagcaagata tcataggtca agatctcata
     1441 ggtcctctcc tcatgtactt catcgtggcg actaaaggaa ccgtcagcct ccttgataaa
     1501 gaaagtcagc tcatgcacga tggagtgagg agcttcgtcc tcatatgtat cccaaagcat
     1561 ggcaaaatct tccgcatttt catggtagga atagccaggg aatacttcat ctgtctggta
     1621 ggtcgagtgc acgtcaaaga tgaaaactcc ctcttcattc agcgcattgt acacgtcctt
     1681 aaagacgtcc cctacttcca cctcatcctg catatagcag atagagtccg aataacacgt
     1741 gacaaaatcg tatttccctg ccttggacaa attcagcata ttaccttcta taaaagcaat
     1801 cttttgcttg gctgaagttg ctctcttctc cgcaatcttc aacatatccg cactcaagtc
     1861 aagtccagtc acatcaaaac cagcctgaga gaagcgcact gattgaattc ctgtcccaca
     1921 agccaattcc aagagtttct ttctctcctt ggtcttaggc aaatgacgca gagaaaaatt
     1981 cgtccatttg tcgtataaac tatcgtccat gaccgcatca tagaccgccg caaaggtttc
     2041 ataagtcgcc ataaaacatg ataccaagtc cgcccgtaat tcgatagaaa attaggagcc
     2101 ttacgatgga gccgagctcc aaggaaggct atctattttc cgagaattac aggacgggtt
     2161 caaatccttt ctaagataca aagagcctta aaagcaaaga gtacatagga aactttcttc
     2221 cctatcgtca gatacaaaga aagttacttt ttatctcagc ttttagctcg tgttcagttt
     2281 cgaaatgaaa caaacacgag gccctttacc tttctatcaa tttttctaaa ataagcaaag
     2341 aaacccagtt gactacaact gagttcatct tctaagcaag agcttctgaa atatctactg
     2401 aattcgcctc atgccatagc ttctctagat tataatgggc acgcatttct tctgaaaaga
     2461 tatgcacgac aacagcaccg aggtccagta agacccagcc tccagctgca tcgccttcga
     2521 catggctacc tttaaagcct gcttgagcta ctttttcacg gatattagct gcgatagcgt
     2581 ccaactgacg gctattcatt gagctagtga tgacaaagta gtccgtcaca ctagtcaaat
     2641 cttgtacatc aagtgcgagg atatcctccg cacgtttctc atcagccgct ttcacgacta
     2701 gttctagtaa ttctttttcg ttcatttaat cctcttttta gaaaatatgt ttgtaatcta
     2761 gaacatctgc aaagcgctcc tcccaaatgt cctcataaaa ttcattaatc gtttctgctg
     2821 gaatgtcatc cccatcaaag gttgtgacag caccttctgg aacaataagc tgatagccat
     2881 attcaaaggc aaccttgaca gaggtatcca cacaatattc tgtctgcata ccacataaaa
     2941 ctaatttttc aatcccctgc ttatccaagt attcttttaa gccagtttct ttgaaaatac
     3001 tgttatactt cttctgaaag accttttcag caggttttcg atttaaaagc gcagataact
     3061 gccaatcttc tgatgtttga gcttcagagt tctcaatatg ttgaacatag ataatttcaa
     3121 tattcttgct tctagcctgg ttttgtaaat aagaaatttt ttccaataga ctttttgtct
     3181 ggaacccagt ttctactaaa acattctgaa catcaatgat tataaaagcc gtcttcattt
     3241 agtcctcttt caaatagtgc acaaaggcgt tataggtttc aagggtttgg ggatagatgg
     3301 ggaatccctg atgagctaga tactccacgg tacgagctgt ttcgtaggcc accgccttat
     3361 tgagcgatag acttgcaatc tcacgcgcca catccactcc aggaaatgcc cgattgtgct
     3421 cgatatagtc tgcgacgtag atgaccttat ctaggtctgt catttgacca gctccgactg
     3481 tatgaatttc aatggctcgc agaatttcag aatcatgcaa atccaaatct tcctgaattt
     3541 tgtaaattcc gaccatacca tgccagacat tgttgcccca gtttttgagg tcagggtcta
     3601 gctggtaacg gtcaatcaag gctagaaatt cctgatctga caacttttta gcatagtcat
     3661 gaagaagacc tgctagactt gctttctcaa catctacacc aaagcgctga gctaattcca
     3721 tagcggcacg ctccacacct agacaatggg ttagacgttt ctcaggtaga agctctgcca
     3781 ttttttccaa caaggcctca cgggagcagt tgatatagtc ttgataggac atcagtagag
     3841 cccctccttc tcgatgtagt ctagcactgg ctgaggtagg agaaagttgg gtttccgacc
     3901 ttgggcaagg aagtcccgca ccatgctgga cgagatatcc atgagcggta cgtccaccca
     3961 gataactgga taggaagtcc ctaccttgta gcgtggacgc tgaaccccca caaactgaac
     4021 catgtcaacc agttcatcaa ttcggtacca cttaggcaga tagtcaacca tgtcggcacc
     4081 gatgataaag taataatccg tatctggatt cttctctgtc aaaatcttca tggtgtcgta
     4141 ggtgtaggaa atacccttgc gctccaactc aatggtttca atgactaggc cgtcaattcc
     4201 ctcaattgcc aactcaagca tcttgagacg atggtgttca gggatggttt cctttttatc
     4261 aacgtgagga ggttggtatt caggcatgag cagaacttga tccagtccca actgttgccg
     4321 tacttgatcc gcaacaataa gatgggcatt gtgaacaggg ttaaaattcc cccctaaaat
     4381 cccaacttgt ttgcgttttt tctccttgat ttctggctcc aactctacct tggtaaaggg
     4441 agtcaatagt tcgattgtca taggctagtc ccctttattt ctctatgaaa acagttattt
     4501 ggagtttcgg tcttagattt ctttgacttt cttagaaatc ttgcgatttt ctttcttgct
     4561 agattgttta aacaaaatca agatgcgtcc tattttttgg actgtatcca caccgatttc
     4621 ttcttccaaa atttcagcta cttcgtggat gttttcatct gtgttttgta agagagtaac
     4681 cttgattaat tcacgcgcat caagagcttg acggacgctg gttttgattt ggtcgttgag
     4741 tccatttttc ccgatttgga tgataggttt gagggtgtgt gcctggctgt tgaggaaggc
     4801 acgttgtttt gatgttaatg acataatttt cttccttgtt ttttgatagt tattttaggt
     4861 ggtgagggac ggtcggaact aattttccaa agatttcatc tttgaaaaat ggatttcctg
     4921 acctcacaat tgagccttgg tctcaattgt gccccttgcc aatctacaga ttggcaatca
     4981 caccacggca ataactatcg ctatatgagc tcactttgtt cgctcagcga ttttgtttta
     5041 aataattgct tttcgtgtga cgacggcgac gccttctggt gcccagacgg cgacttttgc
     5101 tgtgcctgtt acacgaatcc agcctaggcc tgagatgact aggtctgtct tatctttaat
     5161 ggtaaagaca tgctggacta gccttgggaa atcttctttt tccttgctat ttggtggtgt
     5221 cagaagagtt cccaggtgct tatcgtagaa agcactagct ccttcaagct tgctacgatg
     5281 gagtttgagt tcattgtcaa agaaagcagt aaatccttgc ttttctcctg ttataaagtc
     5341 aaagcgtccc aaaccaccta aaaatagggt ttgctcagga ttaagctgat aggtcttagg
     5401 cttgatttcc tttttaggac tgacatactt gaggtttttg gccgtcaaat agtgagccat
     5461 ctggtggcgg tggataattc ccggcgtatc gtaaatataa gatccgtcgt caagcggaat
     5521 ctctattttg tccaaggttg tccctgggaa gcgtgaagta gtgatgacat tctgatcacc
     5581 cgtgatttct tggataatag cattgattag agttgatttt ccaacgttgg tcacaccgac
     5641 cacatagaca tcgcggccct tacggtagtg ttcaatcttg tcaatgactt ccttaatggc
     5701 atgtttattt tgtgctgaag ttaggaccac atcgactgga cgaagacctt cttcatgggc
     5761 acgtttcatg agccactggc taatcttacc agacttaact gacttaggaa ggatatcttt
     5821 tttatttcct accaagagga catcattgcc cgagacgaaa cgtggtaaac ctgggatgac
     5881 agatccatta aaatcaaaga tatcaatgac attgaccact aaagcatcac tgtctcccac
     5941 ctcgtgcaag agcttgagga aatcatcgtt cgtcaactgg acatctgtga tttcattgta
     6001 gtggcggaga cggaaacagc gttggcaata gacttcgcca gtctccaaac ctttttcaag
     6061 tgccgactgg ggggtaaaac caagaccagc tttatctgtc gtctgaatgg ttgctccaca
     6121 accaatacag agaatttctt ccatagttaa attccttttt tatatgtaat cggtccgtac
     6181 ttttcagtga tttttctcat aacacgacgc tcacgagttc ggttaatctg cgttttgatt
     6241 gagtcatgtt ggaccaaggg tttgactaaa attgaccgaa tccctgcacg gtgggctgct
     6301 cgtatatctg tcatgagctg gtcaccaacc atgaccactt cctttttgtc atagtggaat
     6361 tccttcatgg cacggtcaat accaaatgtg aagggcttca aggcccagta aacgtaatca
     6421 atcccaaatt tctcaactgc tcgttgaacg cgttttttgg tgttatttga cactacgata
     6481 atgccaatac ccgcgtcccg aaggtcatgt agccattgct tcatctctgg cgttccatca
     6541 gggttgttcc aagcaatgag ggtattatcc aaatcgacca aaacagcctt gattccctgc
     6601 gcctgcaggc ttgggactgt cagatcatag actgcttcca cagcaaaatc tggtatataa
     6661 ttttcaatcg ccattctggc tccttttctt aacttttttt cgcaattttc cttagtaaca
     6721 aggataaaac aatccacaaa cctgcactag ccaaactgat atagagccaa gcatggggct
     6781 tatctgttaa aggtaaggga acattcattc cgaaaaagcc tgtcacgact gccaaaacag
     6841 ctagcaagac tgaaatgata gtcaaggttg tcaaattgtc attcagatta ttgtttagaa
     6901 tattgttgta agaggctgaa agctgctgta aaatctgaga gattaggtct gtcatggata
     6961 ccagctgatg agcctcaatc atggcatcat caaactgttc tctctcaatc tcatcaaaac
     7021 tacgatacaa ggcatgacct tgaatatgct ctaacaaaat ccgattttgt ttggcagctg
     7081 ccgtcagata aaccatacca gtctccaaat cagacagggc aaagaggttt ttcttggtag
     7141 ttcgctggcg caagaggtca ttgacctcat ccctactctt gtccatctgc tcaatgacag
     7201 gatagtaggc attgctgatg atttccagac tggcaaagag aaacttataa atcgaaagcg
     7261 tgtcatggtt ctccagataa cgagtcatct gttcaatgac ataggcgttc ttggtattac
     7321 taatggtaat caggcgacga tgctcgacaa taaaggtcat gggaaaagtc tcatagtagg
     7381 ccttgtcctt ttttacgtct aggacattat agataaaggt aaccgtccca ctttcacggt
     7441 ggtagtccat gtgggcgcgc tcgtttctat ccagtgcgta ttcaatggtt tccttgtcca
     7501 aaccgtaaat ttcagaaagg tcttctagtt tattcaactt tcctaggtct aggtctatcc
     7561 aggtacaacc attgcccaac tgcttttcta aaaccatctt ttctccttat taaactccct
     7621 ctattgtacc acaaattact taaaataaca ggtctagtct gtgcgtgaga aattgctaac
     7681 aactgtgata tttcggttag ggacaaagtg aagggcttgc tcctcgctac gaagctgtgt
     7741 cgtacgaatt cccacactga caaccttacc cgatacagta atcggtccat ttgtcagaac
     7801 gacctcatct cccacatcca gttgacgttc aaagaggatg aaaaagccat tgatgacatc
     7861 agacagaaag ccttgggctc ccataccaat cgctacccca gcaataccag ctccagccag
     7921 caaactagaa actggcaaac ctaaaatcga caaaatgcag tagagtaaaa agaaatatag
     7981 cgtataatta aacacatttt ctagtaaacg tgagatggtt ttttggcgtc cgacatcatg
     8041 acgagacatt tttagagaag gtttgacaat tctctgcacc atggtatgaa gcattttttt
     8101 agcaatataa aatacaatca aaagcagtaa aagagaaatg accttggtta gaatattctc
     8161 gataatggtt gtcacatcta gcttttcaat ataagcctga ataaattttt gcatataaaa
     8221 ctcctttatt tcattatacc atattttcat cagtctcatt ctccataaat attcaaaaaa
     8281 tagacagaaa attcttgatt ttgtctatca tttatactat aatcataatc actacacaaa
     8341 aaggagatta ttatgaaaag aatcattcat gcttggaata aggcaagcct tgtcaagcgt
     8401 atcttgattg gtatgctttg tggggaatcc taggactgac ccttcctaat ctctcaggga
     8461 ttgggctact cggggattta ttcgttggag gtctgaaagc tgttgctcct atcctagtct
     8521 ttgccctcgt tgccaatgcc ctttcccaac atcaaaaggg acaagatagc aatatgaaaa
     8581 ctgttgtttt cttgtatatc ctatgatagg gactttcgcc gctgctcttg tagctgtact
     8641 agcaagtttc atcgtcccta ttgaaattac cctaaatagt gccaatactg aaattgcacc
     8701 accagatggg attgggcagg tcctcagcaa cctcttgctc aaactggttg acagcccagt
     8761 caacgccctg cttactgcta actatattgg aatcttatct tgggcagtca tttttggaat
     8821 cgctatgaga gaagctagta aaaatagtaa agaattgcta aaaactatcg ctgacgtgac
     8881 ttctaaaatt gtcgaatgga tcatcaatct ggctccattt ggaatccttg gtcttgtttt
     8941 taaagccatt tctgacaagg gagtcggaag ccttgccaac tacggtattt tattggttcc
     9001 attagtaacg actatgcttt ttgttgcccc tgtggtcaac cctttgattg ccttcttctt
     9061 tatgagacgc aatccttact ctctagtttg gaactgcctc cgtgtcagcg gtgtgacagc
     9121 ctttttcact cgtagttctg cgactaacat tcctgtcaac atgaaactct gccatgacct
     9181 tggactcaac ccagatacct attctgtttc tatcccactc ggttctacta tcaatatggc
     9241 tggagtagcg attaccatta accttttgac ccttgctgca gttaacactc ttggaattcc
     9301 tgttgacttt gccacagcct ttgtcctcag tgtggtagca gctatctcat cctgtgatgc
     9361 ttcaggtatt gccggaggtt ccctccttct tatcccagtt gcttgtagcc ttttcggtat
     9421 ttctaacgat attgccatac aaattgttgg ggttggtttt gtgattggtg tcatccaaga
     9481 ctcatgtgaa acagccctta actcttctac agatgtcctc tttaccgccg ttgccgaata
     9541 cgcagcaacc cgtaaaaaat aactcatcaa ggcaagcctg ctcatgtctt gtcttttacg
     9601 cttttattct aacttattag gaaattctta tgtctattag ccaacgtacg accaagctca
     9661 tcttagctac ctgtcttgcc tgcctgcttg cttattttct caatctttcg tcagcagttt
     9721 cggctggaat tatcgctctc ttgagcctat ctgatacgcg tagaagtact ttaaaactgg
     9781 ctcgcaatcg tcttttttct atgcttctag ctctggctat cggtgttcta gcttttcact
     9841 tgagcggatt tcatatctgg agtctcggcc tctatctggc cttctacgtt cctttagcct
     9901 acaagatggg ctgggaaatt ggcatcacac caagcactgt tttggttagc catctcttgg
     9961 ttcaagagtc aacctctcca gaccttctag tcaatgaatt ccttctcttt gctattggta
    10021 caggatttgc cttgcttgtt aatctctata tgccttcacg agaagaggaa atccagcact
    10081 accacacgct ggtggaagaa aagttaaaag atatcctcca gcgcttcaaa tactatttat
    10141 ccagaggaga cggacgcaac cgagcacagc tggtagcaga attagacacg cttttgaaag
    10201 aagccctcag actggtctat ttggatcact ctgaccacct ctttcaccag accgactacc
    10261 atatccacta ctttgagatg agacagcgac aaagtcgtat cctgagaaac atggcccaac
    10321 agattaacac ttgtcacctt gccgccagtg aaagcctgat cttagcgcaa ctcttttcaa
    10381 aaattgcagg tcaactgagc cagaccaatc ctgcttctga tttgctagat gaaattgaac
    10441 gttatctgga agtcttccgg aaccgcagtc tgcccaagac aagagaagaa tttgaaaccc
    10501 gcgccaccct tcttcaactc ctacgtgaag ccaaaacctt catccaagta aaagttgatt
    10561 tttaccaaaa atatagacag taaaaaataa aaccactaaa aaacagagct tgccagcatt
    10621 cttgaaaagt agtataatta ttgcatgcgc aatcatcttg tgacgcagag actgcccgtt
    10681 aatggacttt cttctattta tgactctaaa aagaaaggag acactatgac ctatttggaa
    10741 aaatggtttg acttcaatcg gcgtcagaaa gaaattgaaa gtctcttgga agagaccgtt
    10801 gcccaacaga gcgagcaaag tctgaccttg aaagagttct acctgctcta ctatctggac
    10861 ttggctgaag aaaaatctct acgccagatt gacctgccag ataagcttca tctgagcccg
    10921 agcgcagttt ctaggatggt ggctcgcttg gaagctaaaa attgcggtct actcagtcgc
    10981 atgtgttgcc atcaagatag acgctctagt tttatctgcc tgacaaatga tgggcaaaag
    11041 atactggcct cgctacaaaa gactgtcgaa gaaagcttgg aagcaggttt ggatttcttg
    11101 atttaagatg attttagaaa aagttgcgtg cgcaatcatt tttcttgaca ttctctttta
    11161 taaggagtaa aataaagtta gcattaaaca aaggagtttt aaatatgatt gaaattacct
    11221 atatagatgc cagcaagaac gaaagaactg tgactttcga gtcttatgaa gactttgaac
    11281 gttcgcaaca agcttgcctt atcggcgtcg cagactacta ccctgtccaa aaattaactt
    11341 acaagggtca taatttggac taccatggga cttatggaga tatcttcttc tatctcatga
    11401 aacaagattt aagccaatat aactaaaaaa aggagaaata caatggcaaa agcaattaca
    11461 gatgcaacat tcgaacaaga aacaaaagac ggtttggtct tagtagactt ctgggcaact
    11521 tggtgtggtc catgtcgtat gcaaggtcca atcttggaca aattgtctga agaactttca
    11581 gaagatgtct tgaaaatcgt taaaatggac gttgatgaaa atccaaacac agctcgtgct
    11641 tttggaatca tgtctattcc aactcttctc ttcaaaaaag acggccaagt tgtcaaacaa
    11701 gttgcaggtg ttcacacagc agaacaaatc aaggccatca ttgctgaatt gagctaatca
    11761 aacgagagac caagtacttt ctttggtctc ttttttcttg ccctttgcca tttttcaaaa
    11821 aatatgctag actatagtag attgaaacta gaatagtaca cctctacttc taaaacattg
    11881 ttagaaatcg atttaactgt cctgatcgat ttgtcctgtt cttatttcat tttactatat
    11941 aagtagaata ttacgatgtt tgggatatgc catcgctaaa aaaactcgat ttggtatttt
    12001 ttagctccct aataggagct ttttgcatgc tctgaacatt tcccattttg gaaggagtac
    12061 tatgaaacgt caatcagcct tggtcgtctt tagtggtggt caagattcaa caacctgcct
    12121 tttctgggtt atgcaacact atgaaacagt cgaagctgtc acctttgcct acggccaacg
    12181 tcatcacctc gaaattcaaa ttgctagaga aatcgctaag gaacagggca ttcgtcacca
    12241 tatcctcgat atgtctctgc tgggacaaat cactgctcag ccagactttg cgacgattca
    12301 tatttcctac attcctgaca agctctgtgt cgagtcaaaa tccctcaaac tatatctatt
    12361 tagctaccga aatcacggag atttccacga aaactgtatc aacaccatcg ggaaagactt
    12421 ggtcaacttg ctagaccctc gctatttaga agtctgggga aaattcactc cgcgcggtgg
    12481 catttcaatc gacccctact acaactacgg taagcaagga actaagtatg aggacttggc
    12541 agaacaacgc ctcttccaac acgaccttta tccagagaaa attgacaacc gctaaagtca
    12601 tactcaatga aaatcaaaga gcaaactagg aagttagccg caggttgctc aaagcagtgt
    12661 ttgaggttgt agataaaact gacgaagtca gctcaaaaca ctgttttgag gttgcagata
    12721 gaactgacaa agtcagctta aaacactgtt ttgaggttgc agatagaact gacgaagtca
    12781 gcttaaaaca ctgttttgag gttgcagata gaactgacga agtcagctta aaacactgtt
    12841 ttgaagttgt agatg
//
LOCUS       DAVLVF010000042        12795 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVLVF010000042 DAVLVF010000000
VERSION     DAVLVF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.042"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(425..2152)
                     /gene="ezrA"
                     /locus_tag="VYN66_001255"
     CDS             complement(425..2152)
                     /gene="ezrA"
                     /locus_tag="VYN66_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV1495276.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIDEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2234..4180)
                     /gene="gyrB"
                     /locus_tag="VYN66_001256"
     CDS             complement(2234..4180)
                     /gene="gyrB"
                     /locus_tag="VYN66_001256"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV1495277.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4195..4758)
                     /locus_tag="VYN66_001257"
     CDS             complement(4195..4758)
                     /locus_tag="VYN66_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1495278.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4843..5397)
                     /locus_tag="VYN66_001258"
     CDS             complement(4843..5397)
                     /locus_tag="VYN66_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV1495279.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5408..6631)
                     /locus_tag="VYN66_001259"
     CDS             complement(5408..6631)
                     /locus_tag="VYN66_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1495280.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6668..9118)
                     /locus_tag="VYN66_001260"
     CDS             complement(6668..9118)
                     /locus_tag="VYN66_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV1495281.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPFSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9318..10247)
                     /locus_tag="VYN66_001261"
     CDS             complement(9318..10247)
                     /locus_tag="VYN66_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1495282.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10387..10674
                     /locus_tag="VYN66_001262"
     CDS             10387..10674
                     /locus_tag="VYN66_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV1495283.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10708..12042)
                     /gene="ciaH"
                     /locus_tag="VYN66_001263"
     CDS             complement(10708..12042)
                     /gene="ciaH"
                     /locus_tag="VYN66_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV1495284.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12032..12706)
                     /gene="ciaR"
                     /locus_tag="VYN66_001264"
     CDS             complement(12032..12706)
                     /gene="ciaR"
                     /locus_tag="VYN66_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV1495285.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
BASE COUNT         3884 a         2733 c         2281 g         3897 t
ORIGIN      
        1 tttagaggcc tatttaccgt ggactaaagt tgtacaagaa aagtgcaaat aagaaatctc
       61 cagattagga actatccgtg agttctctag tctggagatt tttcaataga cttcgttatt
      121 ggacggttac caagttactc aaagtacaga tttgaagctg cagataagac tgacgaaatc
      181 actttaatac taaatgaaaa tcaaaaagca aactaagaag ctagcctcag gctgtacttg
      241 agtacggtaa ggcgacgctg acgtgatttg aagagatttt cgaagagtat aaaacactgt
      301 tttgaggtgg tggataaatc ggctgggtaa aaactcaatc tcaggagaaa atgaagtaaa
      361 tctccccaca actatagaaa aagatttgat tctgctcctc acacaataaa atctttttct
      421 tttattaaaa acgaatcgtt tcacgtgttt tctcatatga ggtaacaaag cgattggtta
      481 caccaggctc tgccacttcc aatgcttgag aaatcttgtc aaatgaagcg tgataatcaa
      541 attctttttc aaaaatatct aaagcttcgt taaatgcttc ttgaatgcgt tcatcaaatg
      601 agcgatagcg gttagaatat tgcaagagtt gctctgtcaa agttgcatat tgtacaatat
      661 tataagtttc cgtttctaaa gcttccatat cattcgttgc aatttcaaga actcgggtaa
      721 cagattcaat gttaatcatt ttttgttcta actcaaccat taaatcctcg gtattattgc
      781 ttgccgtaaa gaataacttc aagaaagttt gtggaatacc tggcagattg cgtttttcca
      841 tgtatcgctt gatagtatgg agacgattga cataaacatt ggccttttga cgtgcattaa
      901 tatcatcttt ctcaatttgt gtcaggcgct cactaactga aatttgctca tcttcaatat
      961 cttttagttg agtttgtaaa tcctcaagat tttcttcaag aactgaataa gcttgggttg
     1021 gttcttcttg atttgaagtt acctcaacaa tagctgcctc aaaactctct aattctgtct
     1081 gaatacgacg aacatggctt gcagctgtct caggaagtaa ataggtcttg ttcaaacgtg
     1141 caatatcttc tcccaataaa gtattattct ctttcatatg ttgaaggtaa gttggaagag
     1201 ttgcaagtag attttccact actttctgag cagcaatttc tcgagtaaaa atatcataca
     1261 aggcattgat ttcctcttgt gcctgtccat tctcatattc ggcattatcc aattccaact
     1321 gacgaatatt ctcttggttt ttcttgaatg cttcataaag caagtggaaa cgcgcttcaa
     1381 tatccgtttc aacaaaatga taattagcat caattagttt acgataaccg gattccaaat
     1441 cctgtaattg atctggcaat tctgtagaaa gcgtcgtaac caaggctgga acacgatcca
     1501 caatatgact taaggccaaa atgtgatttt ctgtattatc caaaatcact gcggcttcca
     1561 cagggtcacc cgatgaattc aaggttacaa attgtgaaaa ttcagattgg atattttcta
     1621 attgtttttc aatttcatcc aaggcttgac catactgttc tgaattttca gcaactctat
     1681 gctgaagttc ctcaaataaa tccaaagcat gaagaacacg accactattt ttagattctt
     1741 gcttctctaa gtctgccaaa gcattgcgaa ttgccgcaat atcttcgtca atcaaagtaa
     1801 tttgactctc aatttggtca atttgatgac tggccttgag aaaacgaaat gaatggttat
     1861 agccttctgc ttcaaagaga ttattttcaa tatcggcaaa agagttgaga gataaatcga
     1921 cccatttttg attccattca cggaaagcca cttgactttg tccaatcaag tgcatatttt
     1981 ttacagcttc tacttcatca tttactggaa gattgtatag ttcttctttt ctttcttcta
     2041 gcgcctctaa tctcccctcg tttcgcttac gtagaaagat tgccactaca taagccagaa
     2101 ctaaaatgac tgcaattgca accattaaat aaattagttg tccattagac atatcaaact
     2161 ccttttttac ttgaaacaat cgtaacgatt atatcatatt ttttagacca agggaactac
     2221 ttctccctat tttttagaca tcaagtgtac tatagacagc attttcttcg ataaactcac
     2281 gacgaggctc tactcgatcc cccatcaaca tatcaaagat tttatctgct tctgcagcat
     2341 catctacaga aactctagcc atcaagcgat gttcgggatc catggttgtt tcccacagct
     2401 gatgatcgtc catttcacct agccccttat aacgctgaat agtcggtttg gtacgacctt
     2461 cactataacg ggctaaagct tcttggagtt tgatttcttg atctgcaccc ggctggatat
     2521 attctttaat ctcgcttcca accttgacac catagattgg tggttgggca atataaacat
     2581 aaccagcttc taggattggt ttcatataac gataaatcaa ggttaaaaga agggtacgaa
     2641 tgtgggctcc atcgacatcg gcatcggtca tcaaaacgag tttttggtaa cgggctttcg
     2701 aaacatcaaa ttctgcgcca aatcctgttc ccatggctgt gaaaagacta cgaatttctt
     2761 cgttagctag aatcttatcc atacttgctt tttcaacgtt caaaatctta ccgcgaattg
     2821 gaaggatagc ctgaaactca cggttacgac cagatttggc tgatccacca gctgagtctc
     2881 cttcgacgat gaagagttct gtttcagcag ggttattaga agaacagtct gctagtttcc
     2941 ctggaaggtt ggaaatttcc aaaccagatt ttttacgtgt gacttcacgc gcacgcttgg
     3001 cagccacacg agccttggca gctaaaatcc ctttttccac gatacgtttg gcaatctgtg
     3061 gattttccat gaggaaatcg gagaaggctt cactgaagag gcgattggta atcttgacca
     3121 cttcgctatt tcccaatttg gtcttggttt gtccttcaaa ctgtggattt gggtgtttaa
     3181 ctgagataac tgcagttaat ccttcgcgaa catcttcccc tgttaaatta tcttcattgt
     3241 ctttcagtaa cttattctta cgagcataat cattgataac acgagtcagt gccgtacgga
     3301 aaccttgttc atgcgttcca ccttcatggg tatgaatatt attggcgaaa ctcatgacat
     3361 tttcatggta acctgttgtg tactgcatgg ctacctcaac tgtgatatca tccatctcac
     3421 cgtctgtata gattggtgta tcaaagatta catccttgtt ctcgttgata tattcaacgt
     3481 aactagcaat cccaccttca taatgataat gcttggtttg ttccaaacct tggcgcttat
     3541 ctgtaattga aatttgaaga ccgcgattta gaaaggccaa ctcttgaatc cgtttattta
     3601 atttatcaaa atcaaagatt gttgtttcag tgaaaatttc tgggtccggt gtgaagtgaa
     3661 ccgttgttcc agttctatct gtatccccaa ccacctcaag gtctgcaaca acatgaccac
     3721 gacggtattc ttggtaatga atcttaccgt ttttatggac atgaacgtct aattgagtgg
     3781 aaagggcatt aactactgac gaccccaccc cgtgaagacc acctgaaacc ttgtatccac
     3841 caccgccgaa ctttcctcca gcatgaagga ctgtaaagac ggtctcaaca gcaggacggc
     3901 ctgttttttc ctgaatatcg actgggatac cacgcccatc atccacaaca gtaatcgaat
     3961 catctggctc aataaaaact tgaatatggc tggcaaatcc tgccaaggcc tcgtcaattg
     4021 agttatcaac aatttcccag actagatggt gaagaccttc ttttgaggtt gatccaatgt
     4081 acatccctgg acgcatacga acagcctcta agccctctaa aacttgaatt tgactggcat
     4141 cataatcctg tgcctgcaga tttttgattt cttctgtcat cttattcctt tttcttatat
     4201 gtctaatact tgtcttaaat tcacgatact ggtaaacaag tgggtatcaa gtcctgcagc
     4261 ttgacctgct tcgatatcaa tcggccgatc accaatgaca agaccagagc taatctgata
     4321 cttttctctt aaataaagca tggattcggg atttggcttt ctcttaaagc ctgagctaga
     4381 agtcaccact tctgtaaaat aagctgctat agaggttttt tctaaaattt ccaaaacctg
     4441 atcatttcga tgagagacca aaaaatgacg gccaccttga tttaaaatgt cttccaatag
     4501 gtcagaaact ccttcaaata aaatcgggtg ttcaagctct ctggcttcat tttccttgta
     4561 cttttctaaa aaattctcta aattgggagc gaatgtctca atcgcaaaag gagtagaaac
     4621 ctttaaagct tgatagacac tgtcatggtc ttgtgtgata ccatacagtg ccaatgtttc
     4681 aacaaatgca gctgttgaag tttcataatt atccagtaaa gttccaccta aatcccagat
     4741 gtaatcgtga tatttcatac ccttcattat agcatttttt tatgaaaaaa gcgatgattt
     4801 ccctgagttt tccacataaa aaacgagaga ataactcccg ttttactgat ttttaccaaa
     4861 gacatctcga tagagcattc ctgttcgtaa actctctgcg tcccctccta gttgctccac
     4921 caactcgtag gcaaaggcaa gggctgttga aggaccccga ctggttgtca actgaccatc
     4981 taccactact gtttccttga cgtagtgacc atcaaggatt tgctcttgaa cgccgtcata
     5041 acaagtgtat cgcttatttt tcaatatctc tgcttgattg agggcaattg gtgccgcaca
     5101 aatggctgct agtttcttcc cttcttgctc gaagctttgc aattcttgaa tcaaggtctg
     5161 attatcacgt aaatgtgcag aaccaggcat acctccagga agaacaatca tatcatagtc
     5221 tgataaatct ccatcaaaga catgatctgc tcttacttgg attgcatgcg aacccgttac
     5281 ttgctcttca aaaccaacca tatcacatgt gatattggct cgacgcaaga catctacaac
     5341 tgtcaaggct tcaatttctt caaagccctg agctaacata actgctactt ttaccatgat
     5401 tttctcctta tttaatttgt tttaatacaa cctttttccg tttgaatggg acttttccgc
     5461 tcttttcttc agctaaatta gtctggtaac tcatcgataa aagcaaacca acaccaatca
     5521 aattactgat aatagctgat cccccttgcg aaatgaaagg caagggaatc cccgtcaaag
     5581 gaagtagtcc agtcacagca ccgatattct caaagatgtg gaagagcaac atcataatca
     5641 aacctgtgga aatataagtg tagaactggt tatttgattt aagagtaatc ttcaacatac
     5701 ggtaaatcaa catgagatag agggcaataa ccaggacaga gccaataaag ccaaaatctt
     5761 ctgcaataac cgtaaaaatc atatctgact ctcgaactgg gataagcaga ttcgaagcat
     5821 taaatccctg accaaataag ccaccactcc caatggcaat ctgcccttga gcctgctggt
     5881 aagtcgttgt ttgggcaaac tcaaagggat tgagccaagc caaaatccga ttaatttggt
     5941 aggtcggcat tccaatctgg tgaagaaaag ctcgtccgtc cttgctaata aagatagcta
     6001 agaaaccagc aactcctgtt acagcagtca caaatactgg gataataatt ttccaagaaa
     6061 cccctgataa taaaacgatt cctgagaaaa tggctacaaa aaccaaagcc gtccccaagt
     6121 cactttgaag tgctaaaaga actaggactg gaatggtaaa gagaatcatc cagaaaatta
     6181 acaaaaagtc cagcggaacc gtgcgtctcc attccttatg tttctttgta aattggacaa
     6241 tgacacgagc caacatgagg atataggata tcttcataaa ttctgacggt tgaaataggg
     6301 taattccatt tattgatacc cagtttttgg cacccgttga tgcaactaag cttggattat
     6361 aaaatacaat cggcaagatc ataagtccca agcctaaaat atatagaaag ggggtcacct
     6421 tccaaagaaa ttctgtatta aagagcatga ccacaaaacc aatcacaagc cccaaggcaa
     6481 tccaggcgac ctgctgccct aaaatgggca gaatattatt gggataatca tgactaacgg
     6541 ctatatagat agccaccaca ccgatgacca gtagaaaaaa tactggcaag agcaaactat
     6601 aatcgactct agagtcgaga gaacgtttca tatgtactaa ccttatactt tcatacaata
     6661 ctatttatca aagttcatta aaaaatctat caatagcctc gtcaacttcg gatcgagaga
     6721 tggttttaac agtcgcttct tctgctagag atgctactat ttgtttgccg tatcgttttc
     6781 cgacgattct cctatccaaa ataagagtta aggaacgttg gtattcacgt ctcatacttc
     6841 ttcccaaagc ctgttttaaa cgaataatgg ccattggcaa ttgataatca taaaaggcat
     6901 ttttcccttc ttgattcagt tcttgattaa tctttttcgt caagggttct tgaggatttt
     6961 ggaaaggaag cctcggtaca acttgaatca cagaaggatg gcttgaaaaa tcaactccct
     7021 cccagaaact tgctgcacca agcaagattt gttgttcacc tttttcaaag cgtttcttta
     7081 gctgatgaac atccccattt ttatactggg ccaagtggct aactgtaagt aaatccgata
     7141 ctgctagaag catgtcttta gcggtaaaga gaaccaaaat cggttgctgg aaagcttgaa
     7201 tttccactag taaagcagct acctctctgg catagacttc taaggaggtt tctgttacca
     7261 gagggaaatc tttgaccata accacttctt gttcctgttt tccccgagat tcaatcttga
     7321 caaatttggc ttcaggatag cctaaaaggt ctgccaaaga aaccctctga ctaatctcaa
     7381 gagtagccga tactcccaag acttggcaac tctcaggaag taaagaggac agaagagtac
     7441 ggcctgattt tgtagaagaa atctgaattt tcttttgact cgtttcagtt acttcaagcc
     7501 agtaatcacc ttcagctgta aaatagcgaa ccagctcatc aaagccttcc acttctaatt
     7561 ctgaaaaata ctgatggaga ttgtccagag aatctaagat atttttccta gatttaccag
     7621 actgaaattg ttctatcaag tagagacact caaagcgaat actttctagt atccgttgtt
     7681 gaaccctgtt ttcttctcct actaaagcct tatcaattaa atcgataata gattgtatat
     7741 cgtaggtctc ttgaagcaga ttttctagag ctaacaaaat cttttggact tcatcaataa
     7801 tcagtaaacg gtcactgaca aattcaggat tatcttcaag tctggtcacg agataggcat
     7861 gattagtcac taaaagcttg caagtctctg ccctttcttg actacgtttc caaaaatctt
     7921 ccgtcacaaa taagctctgg gatgataaat tcccatcatg acgaaggtct gctagaaaat
     7981 gttggtaacg gtagagttgc ccgatttcat ccaaatctcc tgtctctgtc tcagtaagcc
     8041 agaccaagac ttgcatttta aagcgtctaa ataagcgatt ttcatcattt tcctgcaagg
     8101 aatgataaaa ggcatccaac ttcagataat tttgtggtcc ctttaagcta tgaatatctg
     8161 tatggaacac ttccttgaga cgtttacctt cttcttccat gatttgattt tgaagaatct
     8221 ttgtcggaac actaagaaca atttgtcgct ctttggattg agataaagcg ggtaagagat
     8281 agccataggt tttcccaatc cctgtcggcg cttgaatcag agagaaaggt tcatctttca
     8341 atagcaagcc aacctcttta gcaaaacttt cttgttcctc cctcacttca aggttcaaca
     8401 gagaaatatt tttagaaaag tcttgagata gttttcgtag ctccagagaa gctgccgttt
     8461 tcttaaaata cagaccttga acttggacca agtctggaga actcaggata gattggttgc
     8521 gataagtttc ctcaataacc aggtaagact catataggag agcgtcagcc atttccagca
     8581 agcgttccaa gagaccttta ggaagctggg tcatcttttc ccgtaaaaaa agaagtaatt
     8641 ctgctgtagc ttgggcatct gaaagggctg tgtgtgcgtg tttaagagga attcctaatt
     8701 ctcgacacaa aatcggcaag ctatattttt ccagttcagg gaaaaagacc tgggccaatt
     8761 cgaccgtatc aacacgaggg tttcttagct catagccttc aaaaaataaa ttttccgcca
     8821 agagattagc atcaaactga acattatggg ctacaaaaat cccatcctcc accaagtcaa
     8881 agatttttct ggcaacttgc gaaaaatcag gtgcttgcgc cagacgttgg tctgtcaatc
     8941 ctgtcagttc tttgatatga gcatccaagg gttcatgtgg attgacatcc gtcgtatagt
     9001 gatcgacgat ttctccatcc tcaatcacga caattcccac ttggataatt ttagccttac
     9061 tacctgtgct agttgcctct aaatccacca caacatagcg attaccaatc gttttcatta
     9121 taaaaaatta taccaaaaaa agggccattt ggcacctgca aacataggat aattttcaaa
     9181 ctcaagaaca ctacttttct tagaaatccc atcgaataga gcattctagc ctgctgattt
     9241 ttttaaaata gtgaagaaat acgaaaaatt gagagaagaa atgttttcat cttccttctc
     9301 tcaacgaatc atgtatttta tttaaccatg tcaggatcgt agtcataaaa tcctgtatca
     9361 aggaaacggt ttttagggtg aagtttacga acttcttcat ccagcaagaa ggcttggtca
     9421 tggctaaagt tgccctcttg aatcaagcta cgcgcttgga taaagagttt tgcaatctca
     9481 acaaagttct gaccaggagt gtagagccct tctagtttcc agcgagtaaa gccatgctcc
     9541 accaattctg ttaatttgat catcaaatca aggtcattgt tggcaaagat atgggtccca
     9601 tgattatctt caaaaatgga atagtggctc tctggatcac ttggctcagc caagaagagg
     9661 tcacgtttat gcgtcttttc atcatcgata tgtgtaaagt tatagtagtt ttgcaagagt
     9721 ggacgtttag aatgatggat gacgctagca ccgtaaacca aaacttcagc aggaatttcc
     9781 aaaatctctg gcattttgaa aagttcagct gatggaattt cacgcgccaa aacagcctca
     9841 gatgcgccag ccttttgtcc ccagaagtta atctgacgac tgctagttac catggttgaa
     9901 gcatcgtaga tggtcttaaa tgaataacca tcgcggttaa ctacgtaaaa gacgcctgca
     9961 tccccaatcg taatatagtc tgtcttgatt tcttccaaga agtctaagaa aggcttgata
    10021 cggtccatca tatcttggtg catgagagca ttgaccgcaa cgatcaattc cttaccagca
    10081 tcatgaacca acttagcgat ttcacgtaat tggtcataac taaaggtcgt tggcagacga
    10141 aggccaaaat ctttctcacc gacatagata cggtctacgc cagcttcgag tagttgttca
    10201 acttgttcaa tactttcagc agttgctgta atgacaatct tttccataag aaaaattata
    10261 ccacatttct ccaaaatcag ccatgctttg aaaatgaaaa gggactttat tctttttgta
    10321 acctttctgt aataattctc tgccgaaaaa atgataaaat aggtatgtac tgttaaggag
    10381 agaatcatgc ccgtaagaaa attacagtcc tatgaggtag actatcaaga agaattaaac
    10441 cagcagcttc ctcattacca agcttataca cctgaagcgc aatctgatgc caatctcaag
    10501 gaaatcctat tttttattaa tatcgctgtt ttttgtatct gtattgctat ctttagtttt
    10561 atctttttag cattaaaatt atcaactgct cttgcctttg ccgcagcaat cggattcagc
    10621 ttacttgttt taaaagttca acggtctatt atcaaacgaa aacgtagaag ataaatccaa
    10681 aatatcgccc caattggagc gatattttta ttttttcttt ttagatggtg tctgaatggc
    10741 aatcttcact tcaaagattg ttcccttggg tttattatct ttgacagtaa cagttccttt
    10801 tagagcatct acaatttgct tggctaggga taatcctaaa ccaaaaccac ctttttgccg
    10861 ggttctagct ttgtctactc gataaaaacg gtcaaaaatt ttctttttat cttctgtcga
    10921 aataccgatt ccattatcag aaacaagtaa ataaagattg cgatcggtcg ccgagataag
    10981 aaaatcaatt tcaccatcct cctcagtata cttgacggca ttatcgaaaa gaatggtcat
    11041 cagttgtttc agaagaagct gatctgtgac aattgttcga tggatacgat tttcaaaacg
    11101 gaagacacga ttattttccg aagcaatcat ctcatagttt gtgaaagttg tattaaaaaa
    11161 gctagttgga acttctgcaa gctccggctt aatcccatca tctctccgag ctaagttcag
    11221 caagcttgtc gttaaaaaac gcatatttcg gacttcttcc aaactcgatg caatgctttc
    11281 gctcacatcc ataatggtag cttctggctt acgaaaaagg gtctctaagc gattttgcaa
    11341 aactgcgagt ggagttcgta actcatgact ggcattttcc acaaaagact gttgcttctg
    11401 catactctca agcaggggcc taacactgac cctagctaga tagagactgg caagtaaaga
    11461 caaaatccag aaactagcca tcacgaccac aatcaattgc tcatgctttt gactagcctg
    11521 ctccaactga ctggtattaa tcaagacagc agcatacttg atattggttg aaaccgaact
    11581 aatattggtc tccatcaaaa tcacacgata gatttcttcc tgcccatagc tattaaaaac
    11641 ctgaatctgg tagatatgtc ctagttcttt cttttctaac ttaatcttat ccaagcccaa
    11701 aaatcgattt ccagaaagaa gttgagtaaa gtctttatca aagagaatga cttccgtatt
    11761 ggaactgaca ttaggtttta tttctacttt actagcgtcc gccctagcat tttctaaatc
    11821 tttaatctct tctgttgccc tatttatagc cagctgaata actgcttgag gattttcact
    11881 cagtccatga agcttatcgt ccaccgaagt ataaagactc gaatgcatga cttgtaaaat
    11941 aatcagagtc attgtagaaa aaatcagggt gaagacaccg aagttgcgga taaaataact
    12001 aaagtcatcc gcataccatg tttttttaag tttactgaac atcttttaaa agatacccaa
    12061 cactacgcaa ggtttgcaaa ttctctgcaa aagtggttcc ctttaatttc ttacggactt
    12121 ttgaaacata gacttcgaca accgaaatcg ttgtatcact atcaaatccc catagacggt
    12181 caaaaatctg cgtcttaggc aaaatcacat tttgattttg aaggaaataa actagtaaat
    12241 cgaactcttt ccccagcaat tcgacaggag tatcttcaac tttaacggta ttggttgata
    12301 aattaaccac gatattccca taagtcaagg tgttttcatt aaacttccct gaacgtttga
    12361 gaagggcctg aattcgcatt ttaagttctt ctaggtagaa aggtttggtc agataatcat
    12421 ccgctcccag ttcaaatcca tgtcccttgt catccaaact ttccttggca gtcataatca
    12481 gaactggtgt cgtaattccc ttttcacgca attcttttaa gacttggaaa ccattttttt
    12541 ctggcaacat caaatccagc aaaatcaagt catagacacc actctcagct tcgtagagac
    12601 cttcttctcc atcaaatacc tgcataacat ccgcaaaatc gtctaaaaag tcaaatactg
    12661 aatttgacag acctaggtca tcctcaacca ataagatttt tatcatgaga aactcctcct
    12721 tattaaaact attataccaa atttgcctta aaaaaaactc aactctctgc attttacatg
    12781 agatagctga gtttt
//
LOCUS       DAVLVF010000043        12724 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVLVF010000043 DAVLVF010000000
VERSION     DAVLVF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12724
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.043"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..472
                     /locus_tag="VYN66_001265"
     CDS             <1..472
                     /locus_tag="VYN66_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1495286.1"
                     /translation="EKIDSGNVLVDKQDIWKIKERTFFKNTVGYVFQNYSLIDNKTVY
                     DNLSLITKDKKTITDVLEKVGLSSDYLHQKIYELSGGQAQRVAIARMLMKPRKIILAD
                     EPTGALDGEIGKEIIRLLLNETAEDKYVIIATHDPAVYNEVDVIIDMKDIGYKV"
     gene            469..969
                     /locus_tag="VYN66_001266"
     CDS             469..969
                     /locus_tag="VYN66_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495287.1"
                     /translation="MKKKIYIALIFVTGVLAIFFFGKQMITKENINKPTVELTIYTLS
                     SSDTEKWNKVRQVETEEAIYFITVKEVSSSEEVFSNIIANGAATGFGVREEEVKKFNN
                     GLGDTIEDSKHNKLIEIEFFTFSDDGAGFVVANFDYGKEELNSQKKDIKELYKKIYES
                     FKEKNK"
     gene            complement(1135..2481)
                     /gene="gor"
                     /locus_tag="VYN66_001267"
     CDS             complement(1135..2481)
                     /gene="gor"
                     /locus_tag="VYN66_001267"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV1495288.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            2746..3945
                     /locus_tag="VYN66_001268"
     CDS             2746..3945
                     /locus_tag="VYN66_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1495289.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            3929..4630
                     /locus_tag="VYN66_001269"
     CDS             3929..4630
                     /locus_tag="VYN66_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495290.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            4632..5891
                     /locus_tag="VYN66_001270"
     CDS             4632..5891
                     /locus_tag="VYN66_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1495291.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            6015..8012
                     /gene="metG"
                     /locus_tag="VYN66_001271"
     CDS             6015..8012
                     /gene="metG"
                     /locus_tag="VYN66_001271"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV1495292.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            8056..8136
                     /locus_tag="VYN66_001272"
                     /pseudo
     CDS             8056..8136
                     /locus_tag="VYN66_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            8343..8663
                     /locus_tag="VYN66_001273"
     CDS             8343..8663
                     /locus_tag="VYN66_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1495293.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            8667..9482
                     /locus_tag="VYN66_001274"
     CDS             8667..9482
                     /locus_tag="VYN66_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495294.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGVFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            9557..10483
                     /locus_tag="VYN66_001275"
     CDS             9557..10483
                     /locus_tag="VYN66_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV1495295.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            10549..10815
                     /locus_tag="VYN66_001276"
     CDS             10549..10815
                     /locus_tag="VYN66_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV1495296.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            10808..11506
                     /locus_tag="VYN66_001277"
     CDS             10808..11506
                     /locus_tag="VYN66_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV1495297.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDAYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEARI
                     QVFGIAPGAVKTAMTAADFETGGLADWVASETPIKRWIEPEEVAEVSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            11517..11996
                     /locus_tag="VYN66_001278"
     CDS             11517..11996
                     /locus_tag="VYN66_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1495298.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGLPELDKTSYMNWKVK"
     gene            12004..>12724
                     /locus_tag="VYN66_001279"
     CDS             12004..>12724
                     /locus_tag="VYN66_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1495299.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEI"
BASE COUNT         3941 a         2143 c         2852 g         3788 t
ORIGIN      
        1 agaaaaaatt gacagtggga atgttttagt tgataaacaa gatatttgga agataaaaga
       61 aagaactttt tttaaaaata ctgttggata tgtatttcag aattattctt taatagataa
      121 caaaacagtg tatgataatc tgagccttat cactaaagac aaaaaaacaa taactgacgt
      181 acttgaaaaa gtaggactgt caagtgacta tttacaccaa aaaatatatg aattgtctgg
      241 agggcaggct caacgtgtag ctattgccag aatgttaatg aaaccacgta aaattatctt
      301 agcagatgaa cctacaggag ctttagatgg tgagattgga aaagaaatta ttcgtttact
      361 attgaatgaa acagctgaag ataaatatgt cattatagcg acacatgatc cagctgtcta
      421 taacgaagtt gatgtgataa ttgacatgaa agatataggg tataaggtat gaaaaagaaa
      481 atttatattg ctttgatctt tgtaactggg gtactcgcca ttttcttttt tggaaaacaa
      541 atgattacaa aagaaaatat caataagcca acggtagagt taacaatcta tacgctttct
      601 tctagtgata cagaaaaatg gaataaagtg agacaagtag aaacggaaga agctatatac
      661 ttcataactg tgaaagaagt atcatcttca gaagaagtat tttcaaacat cattgcaaat
      721 ggagctgcta cggggttcgg agttcgtgaa gaagaagtga agaaattcaa taatggttta
      781 ggagacacca tagaggactc taagcataat aaactaattg aaatagaatt ttttactttt
      841 tctgatgatg gcgcgggatt tgtggtagca aactttgact acggtaaaga agagttgaat
      901 tctcagaaaa aagatataaa agaattatat aaaaaaatat atgaatcttt taaagaaaag
      961 aataaatgat tgtattataa tccttgtatt ttgctatggt ttgttaattg atttttgcat
     1021 atcttacttc aacatactat aaaataaaac ataagaaaac gagtatctcc aactacggaa
     1081 atactcgctt tttgatattt agaagtgttt tttgctttgc ttcttttgtt tcgattaacg
     1141 catggttaca aattcttcag atgcagttgg gtgaattgcc acagttgcat caaagtcagc
     1201 cttggttgct cccattttga tagcaacggc aaatccttga atcatttcat caacgccgta
     1261 gccaattcca tgaagtccga caactttttc ttctgaacca gctgttatga gtttgaaacg
     1321 ggattcttga cggttgcgag tgcaagcaga gtacatagat gcaaagcttg atttataaac
     1381 cttgatttgg tcttgaccgt attctttaat agcttgctct tctgtcaaac caacagttcc
     1441 gatagcaggg tgtgaaaaga caacagttgg aatagttgag taatccattt ttgcagtagt
     1501 ttttccgtta aagagacgtt cagatagggt acgtccggcc ttgattgcaa ctggagtcag
     1561 ttctttctcg cccgttacat cacctagagc atagattccc tcaacaacag tattttggta
     1621 ttcatccact tggataaagc cacgttcgtt cagagtcact ccagcttttt caagttgcaa
     1681 gcccttaacg tttggacggc gacctgtagc ccagataact tggctagctg tgtgactagt
     1741 accatcttcg aaatgaatgg taatgccgtc agtagttttt tctaacttga cagggacttt
     1801 gtgagtgtga agtggtaagt ttgttctttc catttccttg accaaacctt caacgatgta
     1861 ggaatcaaaa ccacgtaaag gacgatcgcg gcgaacaaag agatctgtct tgacaccaaa
     1921 agtgtggagt acgccagcta attcaacggc gatataacca gcgcctagaa tggcaattga
     1981 ctctggaagt tcttcccagg caaatacatc atcagaagag ccacctagct cagcaccagg
     2041 aatattagga atacttggat gggcacctgt agcaatcacg atatgtttag cacgaatcag
     2101 ttcaccattt acgcttacag tatgagaatc tacaaattca gcatgacctt caatcaagtc
     2161 tacaccgttg cgtttaaaac taccatcata agaagaacga gcgcgatcaa tgtaggcttc
     2221 acgattgcga cgtagggttg caaagtcaaa gttaagatca gtagtcttaa agccgtagtc
     2281 ttctccaaat tgatggaaag tctcagcgat ttgcgccccg taccacatga tttttttagg
     2341 aacacaaccg acgttgacac aggttccacc taatttcttt tcctcaataa cggctgcttt
     2401 ggctccatgt tcaccagcac ggttcatggt agcgattcct ccgctacctc caccgatagc
     2461 aatgatatca tattctctca ttgaaaactc ctttgtaatc ttttaaatag tagagcgtct
     2521 tttttgatag ttatatttta ttcttttttt gatttgattg caaaaatctg ctacgcacaa
     2581 aaaattgcaa aaaagcttgc caaataagtc aagatagtgt attatatatc tagtacaata
     2641 gaaaagcaaa attccgaacg attgtttaat cctgaactgt attgtttgct ggagggaaat
     2701 ttggtataat agcaaagtat tgtaaaaaag aaaaggagtt tcattatgaa gaaaaagaat
     2761 ggtaaagcta aaaagtggca actgtatgca gcaatcggtg ctgcgagtgt agttgtattg
     2821 ggtgctgggg ggattttact ctttagacaa ccttctcaga ctgctctaaa agatgagcct
     2881 actcatcttg ttgttgccaa ggaaggaagc gtggcctcct ctgttttatt gtcagggaca
     2941 gtaacagcaa aaaatgaaca atatgtttat tttgatgcta gtaagggtga tttagatgaa
     3001 atccttgttt ctgtgggcga taaggtcagc gaagggcagg ctttagtcaa gtacagtagt
     3061 tcagaagcgc aggcggccta tgattcagct agtcgagcag tagctaaggc agatcgtcat
     3121 atcaatgaac tcaatcaagc acgaaatgaa gccgcttcag ctccggctcc acagttacca
     3181 gcgccagtag gaggagaaga tgcaacggtg caaagcccaa ctccagtggc tggaaattct
     3241 gttgcttcta ttgacgctca attgggtgat gcccgtgatg cgcgtgcaga tgctgcagcg
     3301 caattaagca aggctcaaag tcaattggat gcaacaactg ttctcagtac cctagaggga
     3361 actgtagtcg aagtcaatag caatgtttct aaatctccaa caggggcgag ccaagttatg
     3421 gttcatattg tcagcaatga aaatttacaa gtcaagggag aattgtctga gtacaatcta
     3481 gccaaccttt ctgtaggtca agaagtaagc tttacttcta aagtgtatcc tgataaaaaa
     3541 tggactggga aattaagcta tatttctgac tatcctaaaa acaatggtga agcagctagt
     3601 ccagcagccg ggaataatac aggttctaaa tacccttata ctattgatgt gacaggcgag
     3661 gttggtgatt tgaaacaggg tttttctgtc aacattgagg ttaaaagcaa aactaaggct
     3721 attcttgttc ctgttagcag tctagtaatg gatgatagta aaaattatgt ctggattgtg
     3781 gatgaacaac aaaaggctaa aaaagttgag gtttcattgg gaaatgctga cgcagaaaat
     3841 caagaaatca cttctggttt aacgaatggt gctaaggtca tcagtaatcc aacatcttcc
     3901 ttggaagaag gaaaagaggt gaaggctgat gaagcaacta attagtctaa aaaatatctt
     3961 cagaagctac cgtaatggtg accaagaact gcaggttctc aaaaatatca atctagaagt
     4021 gaatgagggt gaatttgtag ccatcatggg accatctggg tctggtaagt ccactctgat
     4081 gaatacgatt ggcatgttgg atacaccaac cagtggagaa tattatcttg aaggtcaaga
     4141 agtggctggg cttggtgaaa aacaactggc taaggtccgt aaccaacaaa tcggttttgt
     4201 ctttcagcag ttctttcttc tatcgaagct caatgctctg caaaatgtag aattgccctt
     4261 gatttacgca ggagtttcgt cttcaaaacg tcgcaagttg gctgaggaat atttagacaa
     4321 ggttgaattg acagaacgta gtcaccattt accttcagaa ttatctggtg gtcaaaagca
     4381 acgtgtagcc attgcgcgtg ccttggtaaa caatccttct attatcctag cggatgaacc
     4441 gacaggagcc ttggatacca aaacaggtaa ccaaattatg caattattgg ttgatttgaa
     4501 taaagaagga aaaaccatta tcatggtaac gcatgagcct gagattgctg cctatgctaa
     4561 acgtcagatt gtcattcggg atggggtcat ttcgtctgac agtgctcagt taggaaagga
     4621 ggaaaactaa gatgcagaat ctgaaatttg ccttttcatc tatcatggct cacaagatgc
     4681 gttctttgct tactatgatt gggattatta tcggtgtttc atcagttgtt gtgattatgg
     4741 ctttgggtga ttccctatct cgtcaagtca ataaagatat gactaaatct cagaaaaata
     4801 ttagcgtctt tttctctcct aaaaaaagta aagatggttc ttttactcag aaacaatcat
     4861 cttttacggt ttctggaaag gaagaggaaa ttcctgttga accgccaaaa ccgcaagaat
     4921 cctgggtcca agaggcagct aaactgaagg gagtggatag ttactatgta accaattcaa
     4981 cgaatgccat cttgacctat caagataaaa aggttgagaa tgctaatttg acaggtggaa
     5041 acagaactta catggacgct gttaagaatg aaattattgc aggtcgtagt ctgagagagc
     5101 aagatttcaa agagtttgca agtgttattt tgctagatga ggaattgtcc attagtttat
     5161 ttgaatctcc tcaagaggct attaacaagg ttgtagaagt caatggattt agttaccggg
     5221 tcattggggt ttatactagt ccggaggcta aaaggtcaaa aatatatggg tttggtggct
     5281 tgcctattac taccaatatc tcccttgctg cgaattttaa tatagatgaa atagctagta
     5341 ttgtctttcg agtgaatgat accagtttaa ccccaactct gggtccagaa ctggcacgaa
     5401 aaatgacaga gcttgcaggc ttacaacagg gagaatacca ggtggcagat gagtccgttg
     5461 tatttgcaga aattcaacaa tcgtttagtt ttatgacgac gattattagt tccatcgcag
     5521 ggatttctct ctttgttgga ggaactggtg tcatgaacat catgctggtt tcggtgacag
     5581 agcgcactcg tgagattggt cttcgtaagg ctttgggcgc aacacgtgcc aatattttaa
     5641 ttcagttttt gattgaatcc atgattttga ccttgttagg tggcttaatt ggcttgacaa
     5701 ttgcaagtgg tttaactgcc ttagcaggtt tgttactgca aggtttaata gaaggtatag
     5761 aagttggagt atcaatccca gttgccctat ttagtcttgc agtttcggct agtgttggta
     5821 tgatttttgg agtcttgcca gccaacaagg catcgaaact tgatccaatt gaagcccttc
     5881 gttatgaatg agatcaacaa gatggacact cgtctatctt gtttttgtat ggatttccct
     5941 atcgaaattc attaaaaatg tgatataatg aagtgttaga aaaacaggtt tcatttgcct
     6001 ggaaaggaaa aattatgtct gaaaaaaatt tttatattac aacgccgatt tactatccat
     6061 ctgggaaact tcatatcggt tctgcctaca caactatcgc atgtgatgtc ctagcacgtt
     6121 acaaacgcct gatgggctac gatgtatttt atctgacagg tcttgatgaa catggtcaga
     6181 aaatccagca gaaagcggaa gaagctggta ttacacctca agcctatgtt gatggaatgg
     6241 cggttggagt taaagaactc tggcaattac tagatatctc atacgataaa tttatccgta
     6301 caactgatga ctaccatgaa aaagttgtcg cacaagtctt tgaacgctta cttgctcaag
     6361 atgatatcta cttgggtgaa tactctggtt ggtattcagt atcagacgaa gaattcttta
     6421 cagaaagcca gctggcagaa gttttccgtg atgaagctgg aaatgtgact ggtggtattg
     6481 ctccatcagg tcatgaggtt gaatgggttt ctgaagaatc atacttcctt cgccttagca
     6541 aataccaaga ccgtttggtc gaatttttca aagctcatcc tgaatttatc acgccagatg
     6601 gtcgccttaa tgaaatgcta cgcaacttca tcgagccagg tttggaagat ttggcggtat
     6661 ctcgtacaac ctttacatgg ggagtgcctg tcccatcaaa tccaaaacac gttgtctacg
     6721 tttggattga tgcccttctt aactatgcga cagctcttgg ctacgctcaa gacgaacatg
     6781 gtaactttga caagttctgg aatggaacag tcttccatat ggtaggaaaa gatatccttc
     6841 gcttccactc tatctactgg ccaatccttc ttatgatgtt ggatgttaaa ttacctgatc
     6901 gtttgattgc ccatggttgg tttgtcatga aagacggaaa aatgtctaag tcaaaaggga
     6961 atgtcgttta ccctgaaatg ttggtagagc gttatggact agatccactt cgttactacc
     7021 tcatgcgtaa ccttccagtt ggttcagacg gaacctttac tcctgaagac tatgtcggcc
     7081 gtatcaacta tgaattggct aatgaccttg ggaacctcct taaccgtacg gtttccatga
     7141 ttaataagta ctttgatgga caaatccctg cctatgtaga aggtgtgact gaatttgatc
     7201 atgttcttgc tgaggttgca gaacaatcaa tcgcagactt ccatacacac atggaagcag
     7261 ttgactatcc acgtgcgctt gaagcagtct ggactctgat ctctcgtacc aataaataca
     7321 tcgatgagac tgcaccatgg gtcttggcca aggatgaagc tcttcgtgac caattggcaa
     7381 gtgtcatgag ccacttggca gccagcattc gtgtagttgc tcacttgatt gaaccattta
     7441 tgatggaaac tagtcgtgca gttttgactc aacttggttt ggaagaagtt tctagtcttg
     7501 aaaacttgag tttggctgac ttcccagcag atgtgactgt agttgccaaa ggaacaccta
     7561 tctttccacg tctaaatatg gaagaagaaa tcgcctatat caaggaacaa atggaaggca
     7621 ataaaccagc agtcgaaaaa gaatggaatc cggacgaagt tgagctcaaa ctaaacaagg
     7681 atgaaatcaa gtttgaagac tttgacaagg ttgaaatccg tgtcgcagaa gtcaaagaag
     7741 tgtctaaagt agaaggttca gataagttgc ttcaattccg cttggatgct ggtgatggag
     7801 aagatcgtca gattctttca ggaattgcaa aatactatcc aaatgaacaa gaattggtcg
     7861 gcaagaaagt tcaaatcatt gctaacctca aaccacgtaa aatgatgaaa aaatatgtca
     7921 gccagggtat gattctctca gctgaacatg atggcaaatt aacccttctc acagttgatc
     7981 cagctgtacc aaatggaagt gtgattgggt aaaagtagac aggactagtt caggctagtt
     8041 ctgttttttt atttatactc aatgaaaatc aaagagcaaa ctaggaagct agccgcaggt
     8101 tgctcaaaac acagttttga ggttgtagat gaaactgacg aagtcagctc aaaacacagt
     8161 tttgaggttg tagatgaaac tgacgaagtc agtaaccata cataaggtag ggcgacgctg
     8221 acgtggtttg aagagatttt cgaagagtat taactattat gctttacaaa ctagtgtaga
     8281 agtgatatac tataggtgaa gttactagta ggtattacaa aatagattag aagggaaaag
     8341 tgatgaaaga aactcagcta ttaaaaggtg ttcttgaagg ttgtgtcttg gatatgattg
     8401 gtcaaaaaga gcggtatggt tatgagttgg ttcagacttt gcgagaggct ggatttgata
     8461 ctatcgttcc aggaactatt tatcctttgt tgcaaaagtt agaaaaaaat caatggataa
     8521 gaggcgacat gcgcccgtcg ccagatggtc cagatcggaa gtatttttca ttaatgaaag
     8581 aaggagaaga gcgtgtctca gtcttttggc aacaatggga cgatttgagt caaaaagtag
     8641 aagggattaa gaatgggggt taaaccatga agaaaatgaa gtattacgaa gaaacaagcg
     8701 ctttgctaca tgagttttct gaggagaatc aaaagtattt tgaggagttg tgggaaagtt
     8761 ttaatcttgc tggatttctc tatgatgaag actatctcag agagcagatc tatttgatga
     8821 tgctagattt ctcagaagca gaacgagatg gcatgagtgc agaggattat ctaggtaaga
     8881 atcctaaaaa aataatgaaa gagattctca agggagcacc tcgcagttct atcaaagagt
     8941 cccttttgac gccaattctt gtcctggcgg tattacgtta ttatcaacta ctaagtgatt
     9001 tttctaaagg tcctctatta acagtcaatt tgctcacatt tttagggcaa cttcttattt
     9061 ttctgattgg atttggactt gtggccacaa ttttacgaag aagtttagtc caagattctc
     9121 ctaaaatgaa aattggcact tacattgttg ttgggactat agttcttcta gttgttttag
     9181 gatatgtagg aatggcaagc ttcatacaag aaggagtctt ttatattccg gctccctggg
     9241 atagtttgtc tgtctttacg atttcgctag ttatcggtat ttggaattgg aaagaagcgg
     9301 tctttcgtcc atttgtcagt atgattattg cccatcttgt ggtgggttct ctgctccgtt
     9361 attatgagtg gatgggaatt tcaaatgttt tccttacaaa agttattcct ttagctgtcc
     9421 tctttattgg aatctttgtc ttgttccgtg ggtttaagaa gataaaatgg agtgaagtat
     9481 agtcaaaaag ccgttgcaaa gcggtttttt gttataatga agggaagaat gttagaaatt
     9541 aaaggagaaa aatttgatga gatacataac tcttggtcaa gatgacaaag aattatcaga
     9601 aattgttctc ggaatgatga gaataaaaga taaatctgta aaagaagttg aagagcttgt
     9661 agaaacagca ctttctgttg gaatcaatgc cttcgacttg gctgatattt atggtcgtgg
     9721 tcgttgtgaa gaactgttag gtcttgtcct aaaaaatcgt ccagatttaa gagaaaagat
     9781 gtggattcag tccaaatgtg gcattcgcat tgaagaattt acctattttg atttttctaa
     9841 ggactatatt ataaaatcag tagacggtat tttgcaaaga ttgaagattg atcatctaga
     9901 tagcttgctc cttcatcgac cagatgcttt gatggaatct gaccaagtag cagaagcctt
     9961 tgatctcctt tataaacaag gtaaagttcg agattttgga gtttctaatc aaaatcctat
    10021 gatgatggag ttgcttaaaa aagatgtcaa gcagccgtta gctgttaatc agctacaatt
    10081 gagtgcggct tttactccag gatttgaatc agcttttcat gttaatatgg aagatagtca
    10141 agcagctatg cgagatggca gcatttttga atattgccaa ttacacgatg tggtcattca
    10201 agcatggtct gtcttgcaat tcgggtattt taaagggaac tttgttggaa atgagaaatt
    10261 tcaagcttta aatcaagtac ttgatcgttt agctattaaa tatggagtaa cctcttcaac
    10321 tattgccatt tcttggatat tgcgttatcc agcaaaaatg caggcagtcg taggtacaac
    10381 aaatcctaag cacttgagag aagttagccg agcggcaaac tttagcttaa caagaaaaga
    10441 atggtatgaa atttatcttg ctgcagggaa taatttgccg taagtagtag caggtttgaa
    10501 taactcacag tcaaaaagcc gtataaagtg gttttttgct ataattaaat gaagaatgta
    10561 gaattaaagg agaaaaacat gacatttgaa gagatcctgc ctgggttaaa ggctaagaga
    10621 aaatatgtac gaactggttg gggaggtgcg gaaaactatg tccaactctt tgataccatc
    10681 gagcaaaatg ggcttgcact tgaaatgaca ccttatttcc taatcaacgt ttctggagaa
    10741 ggagaaggtt tttccatgtg gagcccgaca gtttgtgatg ttttggcaac ggattgggta
    10801 gaagtgcatg actaaacgtg tactcattac aggagtgagt tcagggatcg gattggctca
    10861 agctcgcctc tttttagaga agggctatca agtttatgga gttgaccaag gtgaaaagcc
    10921 actcttagag ggtgattttc actttttaca gagagatttg accttggact tggaacctat
    10981 ttttgactgg tgccctcagg tggatgtttt gtgtaatact gccggagttt tggatgctta
    11041 caaaccactg ttggaacaaa cggcgcagga cattcaagag atttttgaaa tcaactacat
    11101 tattccagta gagttgactc ggtattattt gacacaaatg ctggaaaata aaaagggaat
    11161 catcatcaat atgtgttcca ttgcttctag cctagcaggt ggaggtggtc acgcctatac
    11221 ttcatccaag catgccttgg ctggcttcac caagcagttg gctctagact atgctgaagc
    11281 taggattcag gtctttggta tcgctccagg agcagtcaag acagctatga ccgctgcgga
    11341 ttttgagaca ggtggcttag ctgactgggt ggctagtgaa accccaatca agcgctggat
    11401 tgaaccagag gaagtggcag aagtcagtct ctttttggcc agtggaaagg catctgccat
    11461 gcagggacaa attctgacaa tagatggtgg ctggtctttg aagtaggagg agttggatgg
    11521 aaatcaaaaa tcactttggg gtctactgtg tttgctttga aaatggaaag ttactttgca
    11581 ttgaaaaaac gagaggccct tatcaacatc ggtatgatct acctggaggc agtcagcaac
    11641 ttggtgaagg actgacggaa acgctgacta gagaagttat ggaagagacg ggatttactg
    11701 ttagaagcta ctctaatcct cgaatctacg atgttttcgt cagggaagag ttaaaaaatt
    11761 ttatggttca ccatgtcata gccttgtatg atgttgaaat gaatgagagt gcacctcaag
    11821 ttacgatttc ggaagctgtg tctgatggtg cgaatgattc acttggatat atttggatgg
    11881 atattcaaga gattacagaa gaaaatgcat cgccattagt cttgaaggtt aagtctgaat
    11941 tattaggatt gccagaactg gacaagactt cttacatgaa ttggaaggtg aagtagaggg
    12001 ataatggaac tatttaaaac atggaagaaa aatatggttc tctatggtct taaatctcaa
    12061 attggaactg tctaccgaaa caatgatagg acaacaagct tttatgatgt tgggaatttt
    12121 ctatacctag caggggagtt ggattccaga ttttgggaag attttgttag aaaatatggt
    12181 ttagattata agattattat ttcagaaaat actaattggc aagattttct gcatcgaaaa
    12241 gtggggctaa attcttttac tcgctattct tttaaagata aggcaaattt tcaagttgaa
    12301 tttttaaata atctagttac tcatttagag gaaggttaca atattgtgcc tattgataat
    12361 catatttata actgcttttc tacggaagaa tggtcacagg atttacaggg ggattttgag
    12421 tcctatcagg attttgtttt aaaaggtgga tttggctttg tgattcttaa aaataatgaa
    12481 ctgattgctg ggatttcctc agggttagtt taccgtaaag cagttgaagt ggaagttgca
    12541 actagaccaa acgaacaagg aaatggattt gctaaaaagc ttggtgctgc aatgattcta
    12601 gagagtttaa atagagatat gtttccactt tgggatgctc ataatgaggc ttccaaaaaa
    12661 gtagcagaat ttttaggata tgagttatct gaaccttatg aagcttttga actagaggaa
    12721 attt
//
LOCUS       DAVLVF010000044        12659 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVLVF010000044 DAVLVF010000000
VERSION     DAVLVF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.044"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            456..749
                     /locus_tag="VYN66_001280"
                     /pseudo
     CDS             456..749
                     /locus_tag="VYN66_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            856..1207
                     /locus_tag="VYN66_001281"
                     /pseudo
     CDS             856..1207
                     /locus_tag="VYN66_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1483..2789
                     /locus_tag="VYN66_001282"
                     /pseudo
     CDS             1483..2789
                     /locus_tag="VYN66_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2829..3839)
                     /gene="tsaD"
                     /locus_tag="VYN66_001283"
     CDS             complement(2829..3839)
                     /gene="tsaD"
                     /locus_tag="VYN66_001283"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV1495300.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(3829..4266)
                     /gene="rimI"
                     /locus_tag="VYN66_001284"
     CDS             complement(3829..4266)
                     /gene="rimI"
                     /locus_tag="VYN66_001284"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV1495301.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYVLAYDGAEVIGFLAVQENIFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(4263..4946)
                     /gene="tsaB"
                     /locus_tag="VYN66_001285"
     CDS             complement(4263..4946)
                     /gene="tsaB"
                     /locus_tag="VYN66_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV1495302.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            5124..5309
                     /gene="cibA"
                     /locus_tag="VYN66_001286"
     CDS             5124..5309
                     /gene="cibA"
                     /locus_tag="VYN66_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV1495303.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            5311..5463
                     /gene="cibB"
                     /locus_tag="VYN66_001287"
     CDS             5311..5463
                     /gene="cibB"
                     /locus_tag="VYN66_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV1495304.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(5786..6001)
                     /locus_tag="VYN66_001288"
     CDS             complement(5786..6001)
                     /locus_tag="VYN66_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495305.1"
                     /translation="MYNLFAFAIGARLVAFIVFVACVYAGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            6281..6514
                     /locus_tag="VYN66_001289"
     CDS             6281..6514
                     /locus_tag="VYN66_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV1495306.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6516..8195
                     /locus_tag="VYN66_001290"
     CDS             6516..8195
                     /locus_tag="VYN66_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1495307.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            8249..8341
                     /locus_tag="VYN66_001291"
                     /pseudo
     CDS             8249..8341
                     /locus_tag="VYN66_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8548..10461)
                     /gene="mnmG"
                     /locus_tag="VYN66_001292"
     CDS             complement(8548..10461)
                     /gene="mnmG"
                     /locus_tag="VYN66_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV1495308.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10471..10926)
                     /locus_tag="VYN66_001293"
     CDS             complement(10471..10926)
                     /locus_tag="VYN66_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1495309.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEQYGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            complement(11067..12188)
                     /gene="mnmA"
                     /locus_tag="VYN66_001294"
     CDS             complement(11067..12188)
                     /gene="mnmA"
                     /locus_tag="VYN66_001294"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV1495310.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
BASE COUNT         3609 a         2946 c         2521 g         3583 t
ORIGIN      
        1 aataaccttt gaatctctag atacgattat caaaatagga tatagagaca tccttctgat
       61 ctgcttttta caaagtccaa ttatatgcgg atctatacct ccacaatgtc cattattata
      121 cctaactata atatgagccg aaaacactat atccttaatg tctccatatc catcagggat
      181 attaatattt atttttccac aactatattg cattgtaacc atctccttaa acgacgcatt
      241 atgatatttg atagagaaat ttttatgaat aactcaataa ttttatagta aatcatgctt
      301 atatctcaaa gatacctatt ttctcttgtc tcgaccttct ccaaagaatt gctataatac
      361 tattacaaat acatctgcac tacacttcaa attttagcac tgtataaaaa cgtttcaaca
      421 cactaacttt aagaaaactt ccactattaa ttgaaaaagt tgatagagat aaattaaaga
      481 tctatattga aactcatccc gatgcttatt tgactgaaat agctgctgaa ttcaactgtc
      541 ctccaacaac tattcattac gctctaaagg ctatgggata tagtctaaaa aagagctgta
      601 cctactgcga acaagaccca gaaaaagtaa atcggttcct taaagaattg aatcacttaa
      661 gctacctgac tcctatttat atttatgaga caggggttga gacctatttt tatctcgaat
      721 atgatcgagc cttgagcagg cagttagtct ctctggaaga agatataatt atttgaatta
      781 agatcgagac aacgcacacc agagattgcg atactgttat agaagtacta atgccctttt
      841 ttgtttcaat atactatggc tccgatgacc tataaagata cgatgacgag tgactttttc
      901 gaagcttgct tccaaaaatt cttactacct actttagata caccatccct tatcattatg
      961 gacaatgcaa ggtttcacag aatgaacatg tgtaaggagc agggcataga ctgttaccac
     1021 ttcctaccta ttcacccgag tataatccca ttgagaaaat atgggcttac atcaaaaaca
     1081 tctcagaata atattgtcaa attacgatgc ttttcttgag gcacttttgt cctattcttg
     1141 tttcagccga ctatactccg ttattgggca gctacggaac agtcgatggg acgatggggg
     1201 gacataaaaa aatcctccag ttttgttttt tataacagta tactggagaa ttgacaatct
     1261 cggtagatac ctcgttatag cgcggttact tattaggcag ttacaaaaca actgtgaaca
     1321 gaaaacattc cagagtcaga caagactttg gaatgttttg gctctataat ttctgtagtg
     1381 ggtaatccca ccccaggaat tatagggtcg tttcttgtag aaaaaaagcc ccatatgacc
     1441 tataatgaaa agcgtctaac caactcatta gaaagggttc atatggaaca acttaagaat
     1501 accacagatt tgctcggatt gaaagacaaa aatatcaaaa tcttgtctgt tctgaaatac
     1561 caaacccatc tagtcgttca ggcaaagttg gattcccccg ctcctccttg tcctcattgt
     1621 caagggaaga tgatcaaata cgacttccag aaagcctcta aaattccgct tctcgactgt
     1681 cagggtttac ccacggtact acatctcaaa aagcgccgct ttcagtgcaa gaattgcctt
     1741 aaggtggtcg tttctcaaac atccattgtc aagaaaaatt gccagatttc caacatggtg
     1801 agacaaaaaa tcgctcagct cctccttgaa aagcagtcta tgactgagat tgcccacaga
     1861 ttggcggtct caacttccac cgtcatccga aaactgaggg aatttaagtt tgaaaccgat
     1921 tggaccaagt tgccaaaagt tatgagttgg gatgagtata gcttcaaaaa gagcaaaatg
     1981 agcttcattg cccaagattt tgagtccaaa tccatcctcg caattttaga cgggcgaact
     2041 catgcggtga ttcgaaacca tttccaacgc tatcagagag aggttcggga gctggtcgag
     2101 gtcatcacca tggacatgta cagcccttat tatcggctcg ctaagcaact ctttccaaag
     2161 gcgaagattg ttcttgaccg cttccacatt gtccaacatc tgagccgagc tatgaaccga
     2221 gtacgaatcc aaatcatgaa ccaatttgac cgaaaatcct tggagtatcg ggcgctcaag
     2281 cgcttttgga accctcgctt tttcgtttct aggctcgggc taaatcagtc cactggactg
     2341 atttactaca ccaggatagc ttcaagctct gtcagaaacg attctatcag cccacgtttc
     2401 gaatgcactt aacccatcgg gaagtacgag ataagctgct ttcttactct gagggattac
     2461 aggttcacta cgaactctat caactcctgc tctttcattt tcaagagaag aatgccgacc
     2521 atttctttgg attgattgag caagaactgc caacggttca tccgcttttt caaacggtct
     2581 tttggacttt tttaagggat agagataaga ttatcaacgc acttaagctg ccttattcca
     2641 acgctaaact tgaagcgacc aataatttga ttaaggttat caagcgcaaa gcctttggtt
     2701 tccggaactt taacaatttt aaaaaacgga ttttgatgac tttgaacatc aaaaaagaga
     2761 gtacgaattt cgtactctcc agattgtagc ttttcgccta cccactacac ttgacaaaga
     2821 gccactcttt attccatggt atcaaaggca agacttggtt tggcattgag gtcccagcct
     2881 gcgaagtttt ctttgttcca ctcgctgacg ctggcatagg caatcatacc tgcattgtct
     2941 ccgcagagtc gcagaggggg gatgataacc ttgacatctg tgatttcggc tgctaggcgt
     3001 tctctgagac ctttattggc tgccacacca cctgccacaa ctagggtttt aacaggatat
     3061 ttctccaaag ccttcttggt ttttgccatg agaatgtcca taactgctgc ttggaaggaa
     3121 gcacacaaat cttctgtaga caggctttct cccttttgct cggcattgtg atgaagattg
     3181 ataaaggcag atttcaaacc tgagaaggag aactccagat tatcttcctt aatcatggca
     3241 cgggggaaat cataaatatc ctgcccctga tgagccagct cgtcaatctc acgacctgca
     3301 ggataggtca agcccatgac acgaccgacc ttgtcataag cctccccaac tgcatcgtct
     3361 cgtgtctccc caacaatctt gtaatctcct gcctccgaaa catagaccaa ctctgtgtgc
     3421 ccaccgctaa ccaagagggc tagcaaggga aactccaaag gctccacact ctgagctgcc
     3481 atgaggtgcc cagccatgtg attaacagga atcagtggaa gtccgtgagc ccaagcaaag
     3541 gccttggcag ctgacaaacc aactagcaag gctccgacca agcctggtcc gtaggtaacc
     3601 gcaacagctg tcacgtcctc ttcggtaatc cctgcttctg ccaatgcctc ctcgatacag
     3661 gctgtaatga cctcgacatg gtgacgactg gctacttcgg gcactacgcc accaaaacgt
     3721 ttgtgactct caatttgact agcaatgaca ttggacaaga gctcatcgtc gtttttcaag
     3781 acggcgacac tggtctcatc acaggatgtc tcaaatgcta aaatatatct atccttcatc
     3841 tatttctctc ttcatgataa tggcgtcctc gactgggtca tggtagtagg cctttcgctc
     3901 agcgatgacc gccatttttt ctttcttgta aaatgcttgc gctcgttgat ttgactttct
     3961 gacttcgagg aaaatctcct tatctgtcgg caattgagca aacaaggctg acgcaattcc
     4021 ctgaccctga taagctcctt taacagcgat ttgcaagact tctgcctcaa agatattttc
     4081 ctgaacagct agaaaaccaa tcacttctgc cccatcataa gccagaacat accaagtctg
     4141 gtcttgggac aggtctgctt ggatttgctc cagagtccaa ggactgacta ggtaaacagc
     4201 tgccataaca gcgtagatgg cttgagctag gtcaggctgt tgttgaattc gcttgatttc
     4261 tatcataggc gtttaatgta ggactcacca gactcggtat ggttcttgag ccagttttcc
     4321 tcagcctcga ctcgtttgag gtaattcggc acaaaatcat gcaaggagtc tgcttccttg
     4381 tcccaagcta aaagagcaag attggctgca ttgggcaatg tttctttgta atcagtcctt
     4441 ggcaagtgtt tttgaatctg ctcaacaaag gggccaactt ctccgacaaa ggttacctga
     4501 ctagtaccct tgactttttc tagcacctct tcaaaagata ggtgcgcttc tgccatgaca
     4561 ggtttggcat tttcataaaa tcctgcataa acattattgc gacgcgcatc catcaagggg
     4621 acaaacaaac cttcttgttg gtggggcacc agagccaaga gactcgacat accaaccaac
     4681 tcgatgttca gggtgtaagc taaggtctta gcagttgcta ccgcaattcg caagcctgta
     4741 tagctacccg gcccttcagc taccacgatt cggtccaaat ccttgggtgt ccaatccaaa
     4801 cttgccatca aaaaatcgat ggcaggcata agagtaatac tgtgattttt cttaatatta
     4861 atcgtcgtct cggcaagaac ctgcttatcc tctaaaatag ccagagaaag agccttgctg
     4921 gacgtatcaa aagctaatac tttcataaca cattcctatc tttttgtctg cttactatta
     4981 tactacaaaa gctggcacat gggaattttc tttgccccca gacaagagtg ccctcactta
     5041 actaaaaata atttaaaaaa atgctcactt ttccttttct tttccgaata taaaagtgaa
     5101 caagaaaaaa ggaggaaagt tcaatgacaa attttgacat tcttgacaat caatttttat
     5161 ccttatctga aaatgaatta tcagatattg atggcggtct cgctcccttg gttatctttg
     5221 gagtagcagt atcttggaag gctattgcag gtggaacagc acttataggt tctggtttgg
     5281 cagctggtta ttttttagga ggagattaat atgatgaaag atttgaacaa ttatcgtgaa
     5341 atttctaata aggaattgca agaaatcaag ggtggctttg gtgtcggtgt tggtatcgct
     5401 ttatttatgg caggttatac cattggaaaa gaccttcgta aaaagtttgg taagtcatgc
     5461 tagataagaa acacattttt agaaggataa attttattgt cttcatctct tacagtttgc
     5521 tcagcattct caatgatttg aacattacta ccatcccttt accattcgat ttatctgttt
     5581 gtattgtttt atttttatgc ttcaactcta tttttgatca gaacaatgac tcccataaaa
     5641 ataataagct ttgaaaattc cattgtcatg tcatgttaga aaaatgcaaa gaccacctca
     5701 tcttgataga tggggtggaa ttttcgtgtc gtaaatctac tatctctaca tccccaaaca
     5761 aaaaacccca gcataagcag ggcatctaag catttaattc aaagtaaaat acaaaccaaa
     5821 cgacataggt cacgaggagg agaaaaagcg agtagagagt cacaaaggtc attttccaca
     5881 agaacttggt ttgtcgtcgt tccagtttgg caaatagaag attccccgca taaacgcaag
     5941 caacaaaaac aataaaagct accaagcgag ctccgatagc aaaagcaaat aagttataca
     6001 tagggcaacc tccttgactt aaaatctata tggaattatg acaagcaata aatttcactt
     6061 ccgttatcaa cataatacat tttctttatt cttgcaaacg cttaccaaag aaatcgtccc
     6121 ctaactttct cgtttccgtc ttttactaat ttttcatttt gtggtataat tgaaataatt
     6181 gtaacgaatc aaggtcaatc tagacacaaa atggaatgaa atcaagcaaa tatctgctaa
     6241 aagtttggaa taagctgacc tgtaaataga aaggaactat atgatttaca aagtttttta
     6301 tcaagaaaca aaagaacgta gcccacgccg tgaaacaaca cgcgcacttt acctagacat
     6361 cgatgccagc tcagaacttg agggtcgcat cactgctcgc caacttgtcg aagaaaatcg
     6421 cccagagtac aatatcgaat atatcgaact cttgtctgac aaattgctcg attacgaaaa
     6481 agaaactggc gccttcgaaa tcacggagtt ctaatatggc ctatactctt aaacctgaag
     6541 aagtcggcgt ttttgccatc ggtggtctgg gagaaatcgg gaaaaacact tacggaattg
     6601 aataccaaga cgagattatc atcgtcgatg ctgggattaa attcccagaa gatgacttgc
     6661 ttggtatcga ctatgtcatt cctgactact cttacatcgt ggacaatatc gaccgcgtca
     6721 aggctgtttt aatcacacac ggacacgagg accacattgg tgggattccg ttcctactca
     6781 agcaagcaaa tgtccctatc tacgctggac cgcttgcctt ggctttgatc cgtgggaaac
     6841 tcgaagaaca cggcctcttg cgcaacgcca aactttacga aatcaaccac aacaccgagt
     6901 tgacctttaa aaatctcaag gcaactttct ttagaacgac tcactctatt ccagagcctt
     6961 tggggattgt cattcatact cctcaaggga aaatcgtctg tacgggtgac tttaagttcg
     7021 actttactcc agttggagaa cctgcggact tgcatcgtat ggctgcgctt ggtgaagaag
     7081 gcgtgctctg tctcctgtct gactcgacaa atgcggaagt accaaccttt accaactctg
     7141 aaaaagtcgt tggtcagtcc attatgaaga ttatccaagg tattgaagga cgtatcatct
     7201 ttgcatcctt tgcctcaaat atcttccgtc tccagcaggc aacagaagct gctgttaaga
     7261 ctggacgcaa gattgcggtc tttggtcgtt ctatggaaaa ggccattgtc aacggaatcg
     7321 atcttggcta catcaaagct cctaagggaa cctttatcga gccaaatgaa atcaaagatt
     7381 atcctgcagg agaagttctt atcctctgta caggtagtca gggtgagcct atggcagctc
     7441 tctctcgtat cgccaacgga acccaccgtc aagtacaatt acaaccaggt gataccgtta
     7501 tcttctcttc tagtcccatc cctggaaaca ctactagcgt caacaagctg attaacatca
     7561 tttctgaagc tggtgtcgaa gttatccacg gtaaagtgaa caatatccat acatctggac
     7621 acggtggtca gcaagagcaa aaactcatgc tctgcttgat taagccaaaa tacttcatgc
     7681 ctgtccacgg tgaataccgc atgcaaaaag tccacgctgg actagcagtg gatactggtg
     7741 ttgagaagga caatatcttt atcatgagca atggcgatgt gcttgccctt actgctgact
     7801 cagctcgtat cgcaggtcat ttcaacgccc aagatatcta tgtcgatgga aatcgtatcg
     7861 gtgaaattgg cgcagctgtc ctcaaagatc gtcgcgatct atctgaagac ggtgtcgttc
     7921 tggcagtcgc aactgttgac ttcaaatcgc agatgattct gtctggtcca gacatcctca
     7981 gccgaggctt tgtctacatg agagagtctg gcgacttgat tcgccaaagc cagcgtatcc
     8041 tcttcaatgc cattcgtatc gcactgaaaa ataaggatgc cagcgtgcaa tctgtcaatg
     8101 gtgccattgt caacgctatt cgccccttcc tctatgaaaa taccgaacgt gaaccgatca
     8161 tcatcccgat gatcctcaca ccagatgaag aataaagcaa gaaaacagcc ccgtcctcgg
     8221 agctgttttt ctctatgctt tcttttgaga ttaaaactca tactcaatga aaatcaaaga
     8281 gcaaactagg aagctagccg taggttgctc aaagcactgc tttgaggttg tagatagaac
     8341 tgacgaagtc agtagccata cctacggcaa ggcgacgttg acgcggtttg aagagatttt
     8401 cgaagagtat caataaaaat cgaaatcaga ctagaaggct aagcgaaagc ataacttgag
     8461 ttagctccca tagttcggga aactatggga ggctggagat gaatcaaagc caagctttga
     8521 actcattcgt aagaagccga cgacgtatca ttttgatttt tgaagagttt tagaaatact
     8581 acgattttta ccttccagat acaccatcaa aatagaaata tctgctgggt ttactcccga
     8641 aatacggctg gcttggccga tggtttctgg attgatgagt ttgaacttct gacgggcttc
     8701 ggttgcgata gaatcaatgt catcccagtc gatattggcc ggaatgcgtt tttcttccat
     8761 gcgtttcatc ttggcaacct ggtccatggc tttggaaata tagccttcat acttgatttc
     8821 tgtttcaatc aattcgataa tcttgtcatc caagtcttct gcagctggtc cgatgaaggc
     8881 caccacatct tggtaagaaa cttctggacg gcgaaggaat tccttggctg tcactgcatc
     8941 ggtcaagggt ttgaagccca tctcctcaac cttggcattg gtttccttga ctggcttaag
     9001 tttgatactg tctaggcgct tcatctcatt atcaaattga tttttcttga tttcaaaacg
     9061 agcccagcgt tcatcgtcca caaggccaat ctcgcgtccc atctcagtca agcgcatatc
     9121 agcattgtca tgacgaagaa tgagacggta ttcagcacga ctggtcaaga gacggtaggg
     9181 ttcaatggtt cccttggtca ccaagtcgtc gatcatcacc ccgatataac catcactgcg
     9241 cttcaaaatc aattcaggct tgccttggat tttcagagcc gcattgatac ccgcgataat
     9301 cccttggcca gcagcttctt cgtaacctga tgttccattt gtctgaccag cagtgaagag
     9361 acctgagatt ttcttggttt ccaaagtcgc acgcaactga tgaggcaaga ccatatcata
     9421 ctcaatagca taacctgtcc gcatcatctc tgcattttcc aaacctttga tggaatgaac
     9481 caagtcacgc tggacatcct caggcagact ggttgaaagt ccttgaacat agacttcctc
     9541 agtattgcgc ccttctggct caaggaagag ttggtgacgt tccttgtccg caaagcgcac
     9601 aatcttgtct tcaatcgacg gacagtaacg aggccccact cccttgacca cacctgtaaa
     9661 cataggcgca cggtggaggt tgttttgaat aatctcatga ctggtaccat tggtataggt
     9721 caaccagcat ggcacttggt ccttgacata atcctcatca cgtgaagtgt atgagaagtg
     9781 attaggcgct tcatctcctg gttggatttc tgtcacgtcg tagttgatag aagaggcctt
     9841 gacgcgtgga ggggttcctg tcttgaaacg accgatttcg agacccagtt ccttgagatt
     9901 gtcagctagg ttaatagaag ccaagctgtg gttaggacct gatgagtact tgaggtctcc
     9961 gatgataatt tccccacgga gagcagtccc tgtcgtcaca ataacagcct tagcagcata
    10021 ttcttgatgg gtggctgtac gcacaccgac aaccttgcca tcttccacca aaatctcatc
    10081 aatcatggtt tgacgaaggg tcagattttc ttggttttca accgtcttgc gcatctcctt
    10141 agagtaaagt tccttgtcag cctgcgcacg aagggcacgg acagctggcc ccttccctgt
    10201 gtttagcatc ttcatctgga tgtaagtctt gtcaatggtt ttggccatct cgccaccgag
    10261 ggcatcgact tcacgcacga caatcccctt ggcagaacca ccgatagagg gattacaagg
    10321 catgaaagcc agcatttcaa tattgatggt cgcaagcagg accttacagc ccatacggct
    10381 agcggccaag gaagcctcaa ccccagcgtg tcccgcacca attacaataa tatcgtattc
    10441 ttcagtaaaa tgataagtca tgtttctctc ctattcctca agatgaatgt gtcttagttg
    10501 gccttcccaa tctggtaggg ctgtttttaa aaaggctgga actagctgga tattctggag
    10561 cttatccaag tcaatccact cacagggctg ccttttctca tcttcctgca tggtcaatgg
    10621 ggcatcttca agcaaatcca ccagataatg aaactcgata ttgtgatagg aaacaccgta
    10681 ttgttcaaaa cgattttcaa ccacaaaagc tagctgccca gcttgagctt taacacccag
    10741 ttcttccttc acttcacgga ctaccgcgtc ttccgtgctt tcattgactt gaatcgcacc
    10801 tccaatagtg taatacttgc ccttgtcttt ggtaactaga agcttgtgat tttggacaat
    10861 caaggctgtc gcccgaacac caaaaaccgt attgtctact tttgtccgaa agtcttgttg
    10921 agtcattctt gtcctttccc ttaaacgaca caaaaacagt caaaactaca aagaagtgca
    10981 ggacaaaaaa gcctgcaaca tccatgagct ttgaccatca tttctattgc tgttattatt
    11041 gtagcaaatt gagaaaattt gtcaatctaa atgtactgac aaacttgtcc atcgcggtaa
    11101 gcattgtcaa tcaaaccacc acctagacac tcttcgccat cgtaaaagac aactgcctgt
    11161 cctggtgtaa tcgcgcgttg tggttccgca aagatgacct ctgccttgtc tcctttgaca
    11221 tggacggtca ccttagagtc aggctgacgg taacggaatt tagccgtaca ttctagcgta
    11281 aactcttctg gcatttcacg agtaaagtgg acttgactgg cttctaggct agttgacatg
    11341 agcgaatcat ggtagaatcc ttgtcctaca tagagaatat tcttgcttag atcttttccg
    11401 acaacgaacc aaggggcatt gtcaccgccg tgttgcccac cgataccgag tccgccacgc
    11461 tgaccgattg tatagtacat aagacctgca tgctcgccca tatcgcgacc atccacagtc
    11521 atcatgcgac caggctgagc tggcaggtag ttgctgagga agtttttaaa gttcttttct
    11581 ccgataaagc aaatccctgt cgagtctttc ttcttagcag tcgaaaggcc tgcttcttct
    11641 gctagttttc gaacttcagg cttttccaaa tgtcctagtg ggaacatggt tttttgaagt
    11701 tgttcttgcg aaagttggct gaggaaatag gtctggtcct tgccattgtc cacgccacga
    11761 agcatgtgaa cagtgccgtc ctcatcacgc gccactcgag cataatgccc agtcgctaca
    11821 tagtctgccc ccaaggttat ggcatagtcc aaaaaggcct tgaacttgat ttccttgttg
    11881 cacataacgt ccggatttgg cgtgcgccct gcacggtatt ccgctaggaa atactcaaaa
    11941 acgcggtccc agtactcttt ttcaaaattg acagagtagt agggaatgcc aatctggtct
    12001 gccaccgcaa ccacatcctt gtaatcttcg gtcgccgtac agacgccgtt ttcatctgtg
    12061 tcatcccagt tcttcatgaa gataccgatc acatcgtagc cctgctcctt gagcaaaaga
    12121 gccgtcaccg acgaatcaac accaccactc atccccacga caacacgtgt tttagagtta
    12181 tcactcatgg taagtctccc atctattcgt ttattcacga ttgaaggtcg tgtgtgcttc
    12241 acgattgaag gtcgcttgag cagtattcat tataacacgc ttggttagag aagacaagga
    12301 agagcttaaa aattgttttt taaaacttat agtcaatata cagattatgt gaatgacgtg
    12361 agtatacctt cttcttatct tcattcaaat aactataata aataaaacaa cccattttct
    12421 ctaatctatt tttcaagtta gagaaaagga gtcgtttttt aatgacattt cctgaaatca
    12481 taagacaggt acctaaagaa tacgaacata aatctattca atcgaacctt atctttgttt
    12541 caaaattaaa atgtcaaatg ttcttaacat gatttaattt aatcggctta aacccattca
    12601 ccgttttcat ttacagtata tccgtctaca gttgtgttga ctgcaagggc acctaagcc
//
LOCUS       DAVLVF010000045        12519 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVLVF010000045 DAVLVF010000000
VERSION     DAVLVF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12519
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.045"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            55..776
                     /locus_tag="VYN66_001295"
                     /pseudo
     CDS             55..776
                     /locus_tag="VYN66_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            967..3258
                     /gene="pcrA"
                     /locus_tag="VYN66_001296"
     CDS             967..3258
                     /gene="pcrA"
                     /locus_tag="VYN66_001296"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV1495311.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNNLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            3301..3981
                     /gene="radC"
                     /locus_tag="VYN66_001297"
     CDS             3301..3981
                     /gene="radC"
                     /locus_tag="VYN66_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV1495312.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQVSV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3978..4667)
                     /locus_tag="VYN66_001298"
     CDS             complement(3978..4667)
                     /locus_tag="VYN66_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1495313.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELTLIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(4685..5326)
                     /locus_tag="VYN66_001299"
     CDS             complement(4685..5326)
                     /locus_tag="VYN66_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV1495314.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(5409..5624)
                     /locus_tag="VYN66_001300"
     CDS             complement(5409..5624)
                     /locus_tag="VYN66_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1495315.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(5632..5979)
                     /locus_tag="VYN66_001301"
     CDS             complement(5632..5979)
                     /locus_tag="VYN66_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV1495316.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5984..7099)
                     /locus_tag="VYN66_001302"
     CDS             complement(5984..7099)
                     /locus_tag="VYN66_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1495317.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(7109..8068)
                     /locus_tag="VYN66_001303"
     CDS             complement(7109..8068)
                     /locus_tag="VYN66_001303"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1495318.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(8181..8750)
                     /locus_tag="VYN66_001304"
     CDS             complement(8181..8750)
                     /locus_tag="VYN66_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV1495319.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8878..9549
                     /locus_tag="VYN66_001305"
     CDS             8878..9549
                     /locus_tag="VYN66_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV1495320.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGVKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            9533..10351
                     /locus_tag="VYN66_001306"
     CDS             9533..10351
                     /locus_tag="VYN66_001306"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV1495321.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLEKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIAHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            10348..11244
                     /locus_tag="VYN66_001307"
     CDS             10348..11244
                     /locus_tag="VYN66_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1495322.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            11288..12262
                     /gene="pta"
                     /locus_tag="VYN66_001308"
     CDS             11288..12262
                     /gene="pta"
                     /locus_tag="VYN66_001308"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV1495323.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         3676 a         2398 c         2695 g         3750 t
ORIGIN      
        1 actgatgcac gatttaagcg tcttgttggt gttcagcgca cgacttttga agagatgtta
       61 gctgtattaa aaacagctta tcaacttaaa cacgcaaaag gtggacgaaa acctaaatta
      121 agcctagaag accttcttat ggccactctt caatatgtgc gagaatatcg aacttatgaa
      181 gaaattgcgg ctgtttttgg tatccacgaa agcaacttaa tccgtcggag ctaatgggtt
      241 gaagtaactc ttgttcaaag tggtgttacg atttcaagaa ctcctctcag ctctgaggcc
      301 acggtaatga ttgatgcgac ggaagtaaaa atcaatcgcc ctaaaaaaac aattagcgaa
      361 ttattcttgt aaaaaaatgc cacgctatga aggctcaagc gattgtcaca agtcaaggga
      421 gaattgtttc tttggatatc actgtgaact attgtcatga tatgaagttg ttcaaaatga
      481 gttgcagaaa tatcggacaa gctggtaaaa tcttggctga cagtggttat caagggctca
      541 tgaagatata tcctcaagca caaactccac gtaaatccag caaactcaag ccgctaacag
      601 ttgaagataa agcctgtaac catgcgctat ccaaggagag aagcaaggtt gagaacatct
      661 ttgccaaagt aaaaacgttt aaaataattt caacaaccta tcgaaatcat cgtaaacgct
      721 tcggattacg aatgaatttg agtgctggta ttatcaatca tgaactagga ttctagtttt
      781 gcaggaagtc tattgaggta ttgagctagt ttatgaaaaa attgggtgaa aagtcgagtg
      841 ttttagaaac ccacagtgta gtattctagt ttcaatccac tatattttgc tactccccgt
      901 aaagtttcta ttttccctga tttctgatat aatagaaata ttgacttcaa gagtaaggaa
      961 gagaagatga acgcattatt aaatggaatg aatgaccgtc aggctgaggc ggtgcaaacg
     1021 acagaaggtc ccttgctaat catggcaggg gctggttctg gaaagactcg tgttttaacc
     1081 caccgtatcg cttatttgat tgatgaaaag ctggtcaatc cttggaatat cttggccatt
     1141 acctttacca acaaggctgc gcgtgagatg aaagagcgtg cttatagcct caatccagct
     1201 actcaggact gtctgattgc gaccttccac tccatgtgtg tgcgtatttt gcgtcgcgat
     1261 gcggaccata ttggctacaa tcgtaatttt acaattgtgg atcctggtga acagcgaacg
     1321 ctcatgaaac gtattctcaa acagttgaac ttggatccta aaaaatggaa tgaacgaact
     1381 attttgggga ccatttccaa tgctaagaat aatttgattg atgatgttgc ttatgctgcc
     1441 caagctggcg atatgtatac gcaaattgtg gcccagtgtt atacagccta tcaaaaagaa
     1501 cttcgtcagt ctgaatccgt tgactttgat gatttgatta tgctgacctt gcgtctcttt
     1561 gatcaaaatc ctgatgtttt gacctactac cagcaaaaat tccaatacat ccacgttgat
     1621 gagtaccaag ataccaacca cgctcagtac caattggtca aactcttggc ttcccgtttt
     1681 aaaaatatct gtgtggttgg ggatgcggac cagtctatct acggttggcg tggtgctgat
     1741 atgcagaata tcttggactt tgaaaaggat taccccaaag ccaaggttgt cttgctggag
     1801 gaaaattacc gctcaaccaa aaccattctc caagcggcca acgaggttat taaaaataat
     1861 aaaaatcgcc gtcctaaaaa tctctggact caaaacgctg atggggagca aatcgtttac
     1921 tatcgtgccg atgatgagct ggatgaggct gtatttgtag ccagaaccat cgatgaactt
     1981 agtcgcagtc aaaacttcct tcataaggat tttgcagttc tctatcggac taatgcccag
     2041 tcccgtacaa ttgaggaagc cctgctcaag tctaacattc cttataccat ggttggcgga
     2101 accaaattct acagccgtaa ggaaattcgc gatattattg cttatctcaa ccttattgct
     2161 aatttgagtg acaatattag ttttgagcgt attatcaacg agcctaaacg tggaattggt
     2221 ctaggtacag ttgagaaaat ccgtgatttt gcaaatttgc aaaatatgtc tatgctggat
     2281 gcttctgcta atattatgtt gtctggtatc aagggtaagg cagcccaatc tatctgggat
     2341 tttgccaata tgatgcttga tttgcgggag cagctagacc acttaagcat tacagagttg
     2401 gttgagtccg tcctagaaaa aacaggttat gtcgatattc ttaacgccca agcgactcta
     2461 gaaagcaagg cacgggttga aaatatcgaa gagtttcttt ctgttacgaa gaactttgat
     2521 gacaccacgg atgtgacaga agaggaaact ggtctggaca aactgagtcg tttcttaaat
     2581 gacttggctt tgattgccga cacagattca ggtagtcagg agacatcaga agtgaccttg
     2641 atgaccctgc atgctgccaa aggtctcgaa tttccagttg tctttttgat tgggatggaa
     2701 gaaaatgtct ttccacttag tcgtgcgact gaagatccag atgaattaga agaagagcgc
     2761 cgtctagcct atgtaggtat cacgcgtgca gagaaaattc tctatctgac caatgccaac
     2821 tcacgcttgc tttttggtcg taccaattat aaccgtccga ctcgttttat taacgaaatc
     2881 agttcagact tgcttgagta tcaaggtctg gctcgtcctg caaatacaag ctttaaggca
     2941 tcatatagca gtggtagtat ttcctttggt caaggtatga gtttggctca ggctcttcaa
     3001 gaccgtaaac gcggtgctgc cccaaaatca atccagtcaa gcggtcttcc atttggtcaa
     3061 tttacagctg gcgcaaaacc agcatctagc gaggcaaatt ggtccattgg tgatattgct
     3121 ctccacaaga aatggggaga gggaaccgtt ctggaagttt caggtagcgg tgctaggcag
     3181 gaattgaaaa tcaatttccc agaagtaggt ttgaaaaaac ttttagccag tgtggctcca
     3241 attgagaaaa aaatctaatt ttccatcctt ctcacgaata ataaagtgag gaggattttt
     3301 atgtacagta tttcattcca agaagattca ctattaccaa gagaaaggct ggccaaggaa
     3361 ggagttgaag cgcttagtaa ccaagagttg ctagctattt tactcaggac aggaacacgt
     3421 caagttagcg tttttgaaat tgcccaaaaa gtcttgaaca atctttcaag cctaacggat
     3481 ttgaaaaaaa tgaccctgca ggaattgcag agtttgtctg gtattgggcg tgttaaggcc
     3541 atagaattac aagctatgat tgaactgggg catcgtattc acaaacacga gactcttgaa
     3601 atggaaagta ttctcagcag tcaaaagttg gccaagaaga tgcagcagga attaggggat
     3661 aaaaaacaag agcacctggt ggcactctat ctcaatactc aaaatcaaat catccatcag
     3721 cagaccattt ttatcgggtc tgtaactcgt agtatcgctg aaccgcgaga gattcttcac
     3781 tatgcaatca agcatatggc gacttctctt atcttggtcc acaatcatcc ttcaggagcg
     3841 gtagcgccta gccaaaatga tgatcatgtc actaaacttg ttaaagaagc ctgcgaattg
     3901 atggggattg ttctcttgga ccatttgatt gtctctcatt ctaattactt tagttatcgt
     3961 gaaaagacag atttaatcta aagttcatta acgacatagt caaagagttt tttatctttg
     4021 ggacgatttt caaaaagaag ttctggatgc cattggacac cgagaaaggc gacatcatcc
     4081 gtactcatga cagcctcaat gataccatct ttaggatcat gagccacaac ttttaaattt
     4141 ggtgctaagt ccttgatgct ctggtggtgg aaggagttga tatgagagat ttctccatag
     4201 atttcttgga gaacggtatc tggttctgtt accaagcgtt gagttgtgta ctcaacagaa
     4261 gaatcctgcc aatggtcttc gatatcttgg tacaaagttc cacccatggc aacgttaaag
     4321 agttgggtac cacggcagac agagaaaatg ggctttttct gtttaatagc ttccttgatg
     4381 agggtcagtt cgaagatatc tctttgaagg tgatagtcat cgctatcaat ggttttgggt
     4441 tcgccataaa attttggatc gacattttgc ccacctgtca agatgagctt gtcaatcaaa
     4501 ctgatatagt ggcaggccat ttcttgatca ccaatcggta ggatgatggg aatccctcca
     4561 gcatctttaa cgccttcaac aaagcctttt gctgcgtagc tcatcatgat gtcatcatct
     4621 ggatgagctt tttcgtttcc tgtaatccca ataactggtt ttttcataaa atgattttcg
     4681 ctttctaatc ctcttttcgc atgaagtaga ggagggtttg gagttcactt gtcaaatcga
     4741 catactgaac gaccacgtct tttggtaaat gcagatggac tggtgaaaaa ctgagaattc
     4801 ctttcacacc agcatcaacc aagagattag caacctcttg tgacttgacg ctgggaacag
     4861 ttaggatagc agtcttcaca tcagcatcct tgattttatc cttgatctga gaaatcccgt
     4921 aaatgggaat cccgtcagga gtttgggtac cgacttcagg atggtcgtct aggtcaaagg
     4981 ccatgataat cttcatcttg ttacgttcgt ggaagcggta gtggagaagg gcatggccca
     5041 tatttccaat accaaccagc atgacattgg taatagagtt gtcattgagc aaatcggcaa
     5101 aaaatgtcat tagttttttg acatcatagc caaaaccacg acgaccaagt tcaccaaaat
     5161 aggaaaaatc acgacgtacg gtcgctgaat caataccgat agcctctgca atttgcttag
     5221 agttggcacg ttcaatcttt tctgcatgaa atctcttaaa aattcgatag tagagagaga
     5281 gtctttttgc tgtagctttt ggaatagcaa actgtttatc tttcacaaaa tcacaacctt
     5341 tctattcttc tattttatag aaacattgtg aaaaaatcaa caaaaataag aaaaaactaa
     5401 gaaaaatctt agttttgatg taaaaaatct gcatgagata gaaaacggta gaggtctccg
     5461 accagaccct gataaacttt ttggccccta aaagtcagag aagtcacata aagtgtatct
     5521 ggtaaggtta cacatcctga caaagtcaac atgagagcct catgatcctc atacttgaga
     5581 gtacgctcta catgatagca gtccttatag gtcagttcaa acattttggc tctatctttc
     5641 cgattttgta aagacaccac gttctaccaa gctatccatg aggaagtaga atttttcctg
     5701 atgaatatgg tggtcttctg atttgaaaat atcaactaga cgaaggccaa acttgtcagt
     5761 gatattgatt ttagcccctg taagttcctt gttaatgatg attttgagtt ggaagccttc
     5821 accgctgttt ggcacttttt ccaaaaggcg agtcagttca tagttaccaa ccttagtttc
     5881 aaaaaaggtg ttatctttga gggtgaattt tttaacagaa gggctaagag tgtaatcgta
     5941 acgacaattt tttaactgaa tgattttttc aaatgccata tggctaacct ccgataattt
     6001 cttttaaggt ttttgcgagg gtttgtaggt cttcaacggt attttgtggc gacaaactga
     6061 tgcgaaggga ttccttcaag cgttctgaat ttgcgccata catggcttca agaacatggc
     6121 tggattggac aacgcctgca gtacaggctg agccagtaga gattgaaatt ccagctaaat
     6181 ctagccgaag gagtaagagg tcatttttct gaccaggaaa tccaatattg agaacataag
     6241 ggagatgatg ttttcctcta ttcaagtaat actgaatgcc ctccagctct gccagaaagg
     6301 cagtttctag attttgtaca tgttgaaaat gttcttcttg tttttctagg tcttctttta
     6361 gggctgcaac catgcctacg atagcaggca gattttcagt tcctgcacgt tttttctgtt
     6421 cctggtctcc gccatgtaga taggaatcaa agtccatgct agatgcgtag agaaaaccga
     6481 ttcccttagg accatggaat ttgtgggcag aagcagtgag aaaatcaatg cccaattctt
     6541 ctgaatgaat tgggatttta ccaatagcct gaactgcatc aacatgatag gcagcagggt
     6601 gttgcttgag tatttggcca atttcagcga tgggcagtag gtttcctgtc tcattattgg
     6661 caaacatggt agaaaccaaa atcgtatcgt cacgtaaagc cttttgaatt tgctgggctg
     6721 tgatttcttg attttctggc tggataatgg ttgcttcaaa cccaaagtgt tgaaccaagt
     6781 aatcaattgt ttcaaggaca gcatggtgct cgatggcagt tgtgatgata tgttttcctt
     6841 gttcttggtg acgaagacag tagccaatga tggtagtatt attgccttca gtcccaccag
     6901 aagtgaaaaa gatatgttga ggttttgtcc ttagtaactg ggctagttcc tgacgggctt
     6961 ctcgcaagag tttgccagct tgacgaccat gaccatgaat actagaagga tttccgtggg
     7021 tttcttgcat aaccttggtc atagctgaaa tagcaactgc tgacatagga gtcgttgcag
     7081 cattgtccaa ataaatcaaa gaatcacctt atttcttttt attgtaggca aagagtgggc
     7141 tgactggttt tctttcgtga atacggacga tagcatcacc aattaactca ctagcagtga
     7201 tgtagcatac atttttagga gttttttctt ttgttgctac tgaatcagtc acaagaattt
     7261 ctttaatatt agtattgtca agaagctcag cagctccctc gacgaagaga ccgtggctag
     7321 aaacagcata aatttctgta gctccttcac gttcaacgat tttagaagct tcagagaagg
     7381 tacgtcctgt atttaaaata tcatcaatca agatagcttt cttaccttca acatcaccaa
     7441 taatataacc ttcgttacga gttgcatcgt cttgagggta gtcgataatg gcgataggag
     7501 catcaagata ttcagccagg ctacgcgcac gtttgacacc tgaattttta gggctaacga
     7561 caacaacatc tgaaccaagc aatcctttat cgcagtaatg ttttgcgaat aggggaacag
     7621 tgaaaagatt atccactgga atatcaaaga aaccttgaac ctgaacggca tgcaaatcaa
     7681 gagtcaggat acgatcaact ccagccttaa ccagcatatt ggcaactagt tttgctgtaa
     7741 gtggctcacg aggacaagca atgcggtctt gacgtgcata gccaaaatat ggaaggacaa
     7801 cgttgatact gtgggcactt gcacgcacac aagcatcgac catgattaac aattccatta
     7861 ggtggttgtt gacagggaaa cttgttgatt gtatgatgta aacatcataa ccacggacac
     7921 tttcttcgat atttacttgg atttctccgt ctgaaaattg acgtgatgat agttttccaa
     7981 gtgggacacc aacagcttgg gcaatttttt gtgcaatctc ttggttagag ttgagtgcga
     8041 aaagtttcat gttttttcta tctgacatta tagaccgtcc tctgtaaact ttataaatcc
     8101 tagttatatt taccttacat atatgaactg ggatttgtgt atttttatct tttctatttt
     8161 accaaaaaat ggagattatt tcagctattt ttcatacttt tgacaaatcg aaccaatttt
     8221 gaaggagctt tttggtagga aatctgattt ttctctaaaa attgtcggaa atcctgtttg
     8281 ccttgctcat gattttccac ttcaagctcc aattcgtaat ctgttatatc aaagtatcgg
     8341 ctctgatcca gtgccatgag accaatagct gttttcattt catagcgaag cgttgttaga
     8401 caaccaagaa cctgccagtt cttactttgg ataccatgtt tcgccaattc atccagtact
     8461 agcccttgag gaagttcttc cttactcaga tagttctcag catcttttag ttgcaatttt
     8521 tggttgtatt ccatgtttcc aacactctgc gggactttga gtgtcaactc agcccagtct
     8581 tcaaaggttc gaatgcgcat agcgactttc ttttctcgca gttcaaaatc aggcgtgtcg
     8641 atgtagtaat ttgtttgaag aacaggagtg acacctgtga actggtcttt tagacgattg
     8701 tattcatctt ttttcaatag tgttttcaat tcaatttcta aatgtttcat ttttcttacc
     8761 tttttttatc gttgaaagcg gatttatggt ataataagca ttgtatttat tgtatatgaa
     8821 tctggagaaa aaatcaaaga tatttttgac ggataatatg agaacaaggg agaatatatg
     8881 accttagaat gggaagaatt tctagatcct tacattcaag ctgttggtga gttaaagatt
     8941 aaacttcgtg gtattcgtaa gcaatatcgt aagcaaaata agcattctcc aattgagttt
     9001 gtgaccggtc gagttaagcc aattgagagc atcaaagaaa aaatggctcg tcgtggcatt
     9061 acttatgcga ccttggaaca cgatttgcag gatattgctg gcttacgtgt gatggttcag
     9121 tttgtagatg acgtcaagga agtagtggat attttgcaca agcgtcagga tatgcgaatc
     9181 atacaggagc gagattacat tactcataga aaagcatcag gctatcgttc ctatcatgtg
     9241 gtagtagaat atacggttga taccatcaat ggagttaaga ctattttggc agaaattcaa
     9301 attcgtactt tggccatgaa tttctgggca acgatagaac attctctcaa ctacaagtac
     9361 caaggggatt tcccagatga gattaagaag cgactggaaa ttacagctag aatcgcccat
     9421 cagttggatg aagaaatggg tgaaattcgt gatgatatcc aagaagctca ggcacttttt
     9481 gatcctttga gtagaaaatt aaatgacggt gtaggaaaca gtgacgatac agatgaagaa
     9541 tacaggtaaa cgaattgatc tgatagccaa tagaaaaccg cagagtcaaa gggttttgta
     9601 tgaattgcga gatcgtttga agagaaatca gtttatactc aatgatacca atccggatat
     9661 tgtcatttcc attggcgggg atggtatgct cttgtcggcc tttcataagt acgaaaatca
     9721 gcttgagaag gtccgcttta tcggtcttca cactggacat ttgggcttct atacagatta
     9781 tcgtgatttt gagttggaca agctagtgac taatttgcag ctagatactg gggcaagggt
     9841 ttcttaccct gttctgaatg tgaaggtctt tcttgaaaat ggtgaagtta agattttcag
     9901 agcactcaac gaagccagca tccgcaggtc tgatcgaacc atggtggcag atattgtaat
     9961 aaatggtgtt ccctttgaac gttttcgtgg agacgggcta acagtttcga caccgactgg
    10021 tagtactgcc tataacaagt ctcttggcgg tgctgtttta caccctacca ttgaagcttt
    10081 gcaattaacg gaaattgcca gccttaataa tcgtgtctat cgaacactgg gctcttccat
    10141 tattgtgcct aagaaggata agattgaact tattccaaca agaaacgatt atcatactat
    10201 ttcggttgac aatagcgttt attctttccg taatattgag cgtattgagt atcaaatcgc
    10261 ccatcataag attcactttg tcgcgactcc tagccatacc agtttctgga accgtgttaa
    10321 ggatgccttt atcggtgagg tggatgaatg aggtttgaat ttatcgcaga tgaacatgtc
    10381 aaggttaaga ccttcttaaa aaagcacgag gtttctaagg gattgctggc caagattaag
    10441 tttcgaggtg gagctattct ggtcaataat caaccgcaaa atgcaacgta tctattggac
    10501 gttggagact acgttaccat tgacattccc gctgagaaag gctttgaaac cttggaggct
    10561 attgagcttc cattagatat tctctatgag gatgaccact ttctagtctt gaataaaccc
    10621 tatggagtgg cttctattcc tagtgttaat cactctaata ccattgccaa ttttatcaag
    10681 ggttactatg tcaagcaaaa ttatgaaaat cagcaggttc acattgttac cagactagat
    10741 agggacactt ctggcttgat gctctttgcc aagcacggtt atgcccatgc acgattagac
    10801 aagcagttgc agaagaaatc tatcgagaaa cgctactttg ctttggttaa gggagatgga
    10861 catttggaac cagaagggga aattattgct ccgattgcgc gtgatgaaga ttccattatt
    10921 accagacgag tggctaaagg cggaaagtat gcccatactt catacaagat tgtagcttct
    10981 tatggaaata ttcacttggt ctatattcac ctgcacactg gtcgaaccca tcaaatccga
    11041 gtccattttt ctcatatcgg ttttcctttg ctgggagatg atttgtatgg tggtagtctg
    11101 gaagatggta ttcaacgtca ggctctgcat tgccattacc tatcctttta tcatccattt
    11161 ttagagcaag acttgcagtt agaaagtccc ttgccggatg atttcagtaa ccttattacc
    11221 cagttatcaa ctaatactct ataaaaactg tctcagagta taattattat cttaaaggag
    11281 aaaactcatg gaagtttttg aaagtctcaa agccaacctt gttggtaaaa atgctcgtat
    11341 cgttctccct gaaggggaag agcctcgtat tcttcaagca acaaaacgct tagtaaaaga
    11401 aacagaagtg attcctgttt tgcttggaaa tcctgaaaaa attaaaattt atcttgaaat
    11461 tgaaggaatc atggatggtt atgaggtcat cgaccctcaa cattatcctc aatttgaaga
    11521 aatggtttct gccttggtgg agcgtcgcaa gggcaaaatg actgaagaag atgtacgcaa
    11581 ggttttggtt gaagatgtca actactttgg tgtgatgttg gtttacttgg gcttggttga
    11641 tggaatggtg tcaggagcga ttcactcaac agcttcaaca gttcgcccag ctctacaaat
    11701 catcaaaact cgtccaaatg taactcgtac ttcaggagcc ttcctcatgg ttcgtggtac
    11761 ggaacgttac ctatttggag actgtgccat taatatcaat ccagatgcag aagccttggc
    11821 tgaaattgcc atcaactcag caatcacagc taagatgttt ggcatcgaac ctaaaattgc
    11881 catgttgagc tattctacta aaggttcagg gtttggtgaa agcgttgata aggtcgttga
    11941 agcaactaaa attgctcacg acttgcgtcc tgaccttgaa atcgatggtg agttgcaatt
    12001 tgatgcggcc tttgttcccg aaactgcagc tctgaaagct ccgggaagta cagtagctgg
    12061 tcaagcaaat gtcttcatct tcccaggtat cgaggcagga aatatcggtt acaagatggc
    12121 tgaacgcctg ggtggctttg cggctgtagg acctgttttg caaggtttaa acaagccagt
    12181 taatgatctt tctcgtggat gtaatgcaga tgatgtttac aagttgaccc tcatcacagc
    12241 agctcaagca gttcatcaat agtgaaaact ataaagtgat atactatgct atactgtagt
    12301 tatgaaacta tgtacgaaaa gcactgccat taattcctga gaactaaatt actgattggt
    12361 gtcaaaaagg aaaacttcca agcgatgata tcctgtctat acacgaccta tagaaatctg
    12421 taatatacat atccgtaaaa cgataaattc cctttttgat tttaaatgag tatgaaaaga
    12481 gaattttttg gctctttgtc aactgtagtg ggttgaagt
//
LOCUS       DAVLVF010000046        12428 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVLVF010000046 DAVLVF010000000
VERSION     DAVLVF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12428
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.046"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(37..384)
                     /locus_tag="VYN66_001309"
     CDS             complement(37..384)
                     /locus_tag="VYN66_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1495324.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKEDYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(495..2270)
                     /gene="spxB"
                     /locus_tag="VYN66_001310"
     CDS             complement(495..2270)
                     /gene="spxB"
                     /locus_tag="VYN66_001310"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV1495325.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2477..4720)
                     /locus_tag="VYN66_001311"
     CDS             complement(2477..4720)
                     /locus_tag="VYN66_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1495326.1"
                     /translation="MTEIVKASLENGIQKIRIQAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSICTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(4730..5101)
                     /locus_tag="VYN66_001312"
     CDS             complement(4730..5101)
                     /locus_tag="VYN66_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV1495327.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFSFACGMNMMHGKMIVE"
     gene            complement(5112..5507)
                     /locus_tag="VYN66_001313"
     CDS             complement(5112..5507)
                     /locus_tag="VYN66_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV1495328.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5691..5789
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5873..6664
                     /gene="thiD"
                     /locus_tag="VYN66_001314"
     CDS             5873..6664
                     /gene="thiD"
                     /locus_tag="VYN66_001314"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1495329.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(6874..7506)
                     /locus_tag="VYN66_001315"
     CDS             complement(6874..7506)
                     /locus_tag="VYN66_001315"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1495330.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7499..8302)
                     /locus_tag="VYN66_001316"
     CDS             complement(7499..8302)
                     /locus_tag="VYN66_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1495331.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8302..8826)
                     /gene="thiW"
                     /locus_tag="VYN66_001317"
     CDS             complement(8302..8826)
                     /gene="thiW"
                     /locus_tag="VYN66_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV1495332.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(8831..9523)
                     /gene="tenA"
                     /locus_tag="VYN66_001318"
     CDS             complement(8831..9523)
                     /gene="tenA"
                     /locus_tag="VYN66_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV1495333.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9534..10184)
                     /locus_tag="VYN66_001319"
     CDS             complement(9534..10184)
                     /locus_tag="VYN66_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV1495334.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10186..11571)
                     /locus_tag="VYN66_001320"
     CDS             complement(10186..11571)
                     /locus_tag="VYN66_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495335.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMESLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11572..12132)
                     /locus_tag="VYN66_001321"
     CDS             complement(11572..12132)
                     /locus_tag="VYN66_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1495336.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
BASE COUNT         3732 a         2779 c         2382 g         3535 t
ORIGIN      
        1 aaaatagcta aggttaacgt caaatgacta cgcgacctat ttcatacgat aaaaatcaag
       61 cactagacca gcaggtcctt gaactaataa ggactctgtt ccccaatcgg ttacagttgg
      121 tccgtgtaaa acctttatac caagctcgtt caaccgtttg tagttctggt ctacatcctc
      181 aacctcgata tgaataatga ttcctgactg aaagttttcc aaaggaacca aatgattttg
      241 tgacaacata aggcagtgac taccaatcgt aaactgagca aaaccatcat cagcataatc
      301 ttccttttta tccaaaatat gctccaagtc agcacagact tggggaacat ttgaaacgat
      361 aatatctaat tgatttaaat tcatttactc tcctccataa aaagaccgga ttgctccgat
      421 cttttaaagt tctgctctat gaaaatcaaa gaataaagtc tacaagtttc atatttgatt
      481 ttcggcgaga ggaattattt aattgcgcgt gattgcaatc cttcttcttc caagaagaga
      541 cggaatggta cgagttcttc tgcttcgtat ttttccttga aggctttgat agcttcttct
      601 gagtgaagtt ttggatccaa ttcaagtact tctactggaa gtggacggtg ttgagtgatg
      661 cgagcatcga tgacaacagt tttaccttct ttgttcaatt taacagcttc tgcaacaact
      721 gcatcgatgt cttcgatacg gtcaactgtg aatccaacag ctccttgagc ttccgcaatt
      781 ttagcgtagt cagcgtttgt gaagtctaca ccaaacaagt gtttgtttgt atcttcgtat
      841 ttgttcttga tgaagccgta ctcagcattt gagaagacaa ggttgataac tggaaggtcg
      901 tattgaacgt ttgtgataac gtctgggtag cacatgttga atgctccgtc acccatgatg
      961 ttccatactt ggcgatctgg attgtctttc ttagcagcga taccaccagg aagggcaata
     1021 cccattgtcg caaagagtgg agatgtacgc cacatgttct taggtgtcat gtgaaggtga
     1081 cgagtagatg tttgagtagt gttacctacg tcgattgagt agatagcgtc ttgatcagca
     1141 tgtttgttga ttgcattgta aacttgatac aattgcaatt caccctcagt tttaccttcg
     1201 agtttgttca tgtaatcacg ccagttttgg ttgttcttaa cgtttgcacg ccaccatgga
     1261 gttgattcaa ctgggtttac tttgtcaagg atagctttag ctgcttgacc agcatcacca
     1321 aggattgaag cgtcaagggc atgacgttta ccaagtttgt aagggtcgat atcaacttgg
     1381 atgaattttt cagtgttctt gaatgcttcg taaacttcag caaatgggaa gtttgaacca
     1441 aggaaaagaa ctgtgtctgc ttcaaagacc acttcgttgg ctggtttcca accaacacga
     1501 taagcagaac ctgtcaaacc ttcatagttc cattcgaaag cttcaaagtt tttaccagtt
     1561 gtgatgattg gtgctttgat tttacgtgac aattcagtaa tcacttcacc agctttaaca
     1621 ccaccaaatc cagcatagat aactgggcgt tcagcattgt tcaagatttc aacagctttg
     1681 tcgatttcaa cttcgttcaa agcaggagcg atgaatgagc gttcgtatga acctgaaccg
     1741 tagtatgagt tttcgtcgat ttcttggaaa ccgaagttta ctggaatttc aacaacagct
     1801 ggaccttttt tagaaactgc agcacggcag gcttcgtcaa ttacttttgg caattgctca
     1861 gcgtaagcta cacgtttgtt gtaaacagcg ataccgttgt acattgggtt ttggttaagc
     1921 tcttggaaag catccatgtt caattcgtta actggacgtg atccaaggat tgctaggaat
     1981 ggagtgttat ccatagctgc atcgtaaaca ccgttaatca agtgagtcgc acctggacca
     2041 cctgaaccaa ctgcaacccc gattgagccg ccgaatttag cttgcataac cgctgcaaga
     2101 gcacctgtct cttcgtggcg aacttgtaag aagcggatat ctttgtcttc agccaaagcg
     2161 tccatcaatg agctgagtgt tcctgatggg ataccgtaga ttgtatctac gccccatgtt
     2221 ttcaatacgt taagcattgc tgcagatgca gtaattttcc cttgagtcat aatgataact
     2281 ctccttcaat ttttttagac ttggagaata cgattacata gaattggaaa cgttctccaa
     2341 ttttttacta ttccactgta tcatatttat gctgactttt ctaaaaatct gctcaaaact
     2401 ctctattctc tattctaata cagttttgaa agtgtcgtca tttctgtttt ataacaaaaa
     2461 aatctagtca ttacttttag tctattttac taaaatttaa cagaagggaa ctggtcagaa
     2521 cagatacaga actaaaggcc atggctagac ctgccaattc tgggttgaga gccagtccaa
     2581 cacctgaaaa gactcctgct gcaatcggaa ttccgacaac attgtagata aaagcccaga
     2641 aaagattgag cagaatacga tgaaaggttt tcttactcat gtcgaaggca cgcaccactc
     2701 ctaaaagatt attggttgtc aacaccaaat ctgctgactc gatggcgata tctgttccag
     2761 ctcccatagc aatccccaca tctgctacac taagggcagg agcgtcattg atcccatcac
     2821 caacaaaggc tactttacct gactgttgca gtttatggat ttcatgggct ttttcttctg
     2881 gcaagacgcc tgcaatgacc tcttcaattc cgatttgatc tgcaatagca cgcgccacac
     2941 cagcattgtc tcctgtcagc atgactgtcc cgagaccacg tttttttagc tgactgatgg
     3001 ctagcttagc atttttctta ggaatatctt gcaaagcaag caagcctttg atttcattgt
     3061 caacagctaa gaacacaact gtcttagctt ctttttctag ttcttctagt ttatcttgat
     3121 aagtattaga aatatccatg ccatccagca ttttagcatt tccaagtaaa acttgttttc
     3181 cattgattcg ccctgaaaca cctttcccgt gcaaggcctg aaaattttca acagtttgaa
     3241 actcaagtcc agcttcactc gctcgcttaa cgatagcctc agccagtggg tgttgagaag
     3301 catcttccaa ggaggctgcc aatccaaaca cttctacttc gtcgccgatg acatctgtta
     3361 ccacaggttt cccttccgtc aaagtcccgg tcttatcaaa gacaagggtt tgaactttct
     3421 ggatttcctg taagacagtt ccatttttga ggagaacccc catcttggca ctacgtcctg
     3481 tccccaccat aagggctgtc ggtgttgcaa gtcccaaggc acaaggacag gcgataatca
     3541 aaaccgccac tccgtagaga agagaggaca caaagctagc tccaagcaca accacactat
     3601 ccctgagcaa gacgaaccaa acccaaaagg tcatgattcc taaaatgaca actactggga
     3661 caaaaatccc tgaaatctta tccgtcaagt cctgaatcgg cgcacgactt gtctgagctt
     3721 tcttcacaaa atccacaatc tgagccaaaa cagtctctga gccaactttt tctgctctaa
     3781 agacaagcgt tccactatga ttgatggttg agccaatgac agtatctcca actgtcttgt
     3841 ccacaggcag actctcacct gtcaccatgg attcgtcaat actagagaca ccttctacta
     3901 cgacaccatc aacagcaatc ttttcaccgg gacgcactcg aatcaggtcg cctaccttga
     3961 cttgttccaa aggaacttgg acataactat catcactcaa gacttctgcg gttttagctt
     4021 gcaagtccag taatttctcc acagcttggg acgtattttt tctcattttt tcctcaaaaa
     4081 ctgctcccaa aagaacgaaa aagaggataa atccagcact ttcgaagtaa acagggagac
     4141 cagcaaagag agcaactagg ctatagaaat aagccactag agttcccagc gcaaccaagg
     4201 tatccatgtt ggcattgtgc tttttaaaac tggcccaagc actctggata tatggcttac
     4261 ctgcaactaa cataataggc gttgttgcta gaaaggttcc ccaatgcatg acttgatgac
     4321 taatgctacc tgccaacatc ccaatcatga gaatcacaag aggcacagta cagatactag
     4381 taatccaaaa acgttgcagg agagatagag attttcgagt cttctcaacg actgtatagc
     4441 ttcccttttg catcttcatg ccacaagaaa attcatgtcg ccctaattct tgaggtgtaa
     4501 aacgaatgac tttctcctca tctacgccga ttggttccaa gataccttct tcttcaaaca
     4561 gaatttcctt ataacagttt gaaggagtag cacgatgaaa ggtaatctca gctggaattc
     4621 ccttttgaag ctggatatgg gctggatgat agcctttttc agcttggata cggatttttt
     4681 gaatgccatt ttctaagctt gctttcacaa tttctgtcat agtctccacc tactctacaa
     4741 tcatcttgcc gtgcatcatg ttcataccac aagcaaagct aaactctcca gcctgttcag
     4801 gcgtgatttc cactacatac tcttccccca ttggcaggtt cgcatgtaca ccaaaatctg
     4861 gaaaaacaat ttgatccaga catggtgaag gatccttgcg gtcaaagaca atgcgggctg
     4921 gcactgattt cttgaggaca atcaactcag gagtatagcc tcccatgact tccactcgaa
     4981 tctcttggta tccgtttttt tgctgggctt tttgtccaga tttttcaggc tttttgaaaa
     5041 accaaaacaa gataaacgcg ataagggcaa tacaaataat ggttacaata ctatttaaca
     5101 tgacgtctcc tttacataca attacatctt acttctgtta cagcacttga tttcttctct
     5161 gaaatcacag cttccaagtc ttccaagtca gtctgagtaa attcacattc tacaatcaag
     5221 tcagccaaca aattcctaat cctacgggaa caaaccttgt ctttgatatc ttggacaagt
     5281 aaatcccgac tttggtctag agttaaaagc gctgaataaa caaaggactt gccttctttt
     5341 ttccgagtca aacactcttt ctcaactaga cgagccaaaa gtgtctgaac cgtggacttg
     5401 gaccagtcaa accgctctgc caaaacccta atcaaatctg tactggtctg ctccccctgc
     5461 atccaaataa tcttcatgac ctgccattct gcatctgaaa tctgcattac catacctcca
     5521 aaatctacat ttgtcaatta cactcaacag tatactccca aaatctacat ttgtcaatta
     5581 tagaaataat attttcttcg aaaaatagaa ttttaatcat ttgaaaaacg atttgcagtc
     5641 aaatattact atataaacaa taaaaatatg ctatactaaa gaaaaaagaa aacaaccact
     5701 aggggtgcgt aaagctgaga ttaacgactg ttagatccct ctgactcaat ctaggtaatg
     5761 ctagctgatg gaagtggaaa tgataatggg gactagcagt cttctattgc ctttctaaaa
     5821 cagactagct tgttcttaag aatacaaact tcagttggtt gggaggtttt agatgactta
     5881 tttacccgtt gctttgacca ttgcagggac tgaccctagt ggtggtgctg gcattatggc
     5941 agatttaaag tcattccaag cgagagatgt ctatggaatg gctgttgtaa ccagtcttgt
     6001 cgctcaaaat accagaggtg ttcagctaat cgagcacgtt tctcctcaaa tgttgaaagc
     6061 ccaattggag agtgtctttt ctgatattcc acctcaggct gtaaaaactg gaatgttggc
     6121 tactactgaa atcatggaaa tcatccaacc ctatcttaaa aaactggatt gtccctatgt
     6181 ccttgatcct gttatggttg ctacaagtgg agatgccttg attgactcaa gtgctagaga
     6241 ctatctcaaa acaaacttac tacctctagc aactattatt acgccaaatc ttcctgaagc
     6301 agaagagatt gttggttttt caatccatga ccccgaagac atgcagcgtg ctggtcgcct
     6361 gattttaaaa gaatttggtc ctcagtctgt ggttatcaaa ggcggacatc tcaaaggtgg
     6421 tgctaaagat ttcctcttta ccaagaatga acaatttgtc tgggaaagcc cacgaattca
     6481 aacctgtcac acccatggta ctggatgtac ctttgctgca gtgattactg ctgaactagc
     6541 caagggcaag agtctttacc aggcagttga taaggccaag gcctttatca caaaagctat
     6601 tcaagatgcc cctcaactcg gtcatggttc tggtccagtc aaccatacaa cttttaaaga
     6661 ttaagaaaaa aaactctcta gttcccactt taagggaatt agagagtttt tatactcttc
     6721 gaaaatctct tcaaactacg tcagcttcca tctgcaactt caaaacactg ttttgagctg
     6781 acttcgtcag tcttatctaa aacctcaagg cagtactttg agcaacctgc gactagcttt
     6841 ctagtttact ctttgatttt cattgagtat taattaggaa agaatgtcat gcaactttgt
     6901 taaaaaggct tgcgtttttg cctcaatatc ttctgcttgc atcaaatcac gtacaacagc
     6961 tacaccagct atgccagtgc ccataagctg atcaatattc tccgaagtca agccaccaat
     7021 agcaactatt ggaatggcaa ccgtttggca aattgttttc aaggttgaaa tcagggtgat
     7081 gggtgcattt tccttggtgg ttgtcggaaa aatggctcct gtccccaagt aatctgcacc
     7141 tgatttctcc gcttccagag ctcttttaac cgttttagcg gtgacaccga ggattttttc
     7201 aggacccaag acttgtcggg caaccgaaac tgggagttcg tcatctccaa tatgcagacc
     7261 agcagcatca accgcaagac aaacatccaa ccgatcatcg attatcaagg gtacctgata
     7321 agcatctgtt atttccttga cttgttttgc cagttgataa tattgattgg ttgtgagatt
     7381 tttttctcgc aattggacta tggtaacccc tgaacggcag gccgtctcaa cttttgcaag
     7441 aaagctttcc acggaatctt gatagcgatt ggttaccaga tatagtctaa gtgcttctct
     7501 attcataaac ctctcctttg atggtatcta gccaattttc atctcttctt aggagcgaaa
     7561 gctgattgag tacttggtaa cgaaattctt ccaatcccat tccttgaaca actattttct
     7621 cagcagcgat attgagataa gagactgcta agcaagaagc ttcaaaacca gtctttcctt
     7681 ggctgagaaa aacggctgtt aaggctccaa ccaaatctcc tgtccctgtt atccagtcta
     7741 attctgcaca gccatttccc agtacagcga cctgattttt cgaaacgacg aggtccttgg
     7801 gacctgtgac taagaaagac ataccatgat aggtctgaca ccagtctttc aagacttgaa
     7861 gcaaatcctc cgtttcttga tctttagcac tcgcatcgac cccaacgccg tggtgcttta
     7921 atccaacaag acttcgaatt tctgacatgt ttcctttaag gaccgtaggt ctatagtcta
     7981 aaaggtctct gactaagctc ttacgaatgg atgaagctgt tacgccaacc gcatctacta
     8041 ccattgggag acaaacttga gctgcataag aagctgctat acggattgct ttctccttct
     8101 cagctgacaa atgccccaaa ttgatgaaga gagcctgact ttgcttagta aaatcaagaa
     8161 cctcacgggg atcatctgcc atgacaggtt tgcatcccag agccaaaatc ccatttgcca
     8221 gcatctcaca agaaatctca ttggtcatac agtgaatgag ggaactagag cctataggaa
     8281 agggatttgt aaattcctgc atcagtctat cctttcacta aagaaatatc cctgcacttt
     8341 tttaaagaat tcctgcttga ttaaaaatcg aaaggcaata aaggaaatcg ctgtaccaat
     8401 caaggttgct ccgaaaaatc gaggcgtgta gataaaccag ctaagcttag cagctgatcc
     8461 tgtaaagagt accataacag gataggaaac aatagaacca ataatacctg ttcccacaat
     8521 ttctcccaag gcagaaaagt aaaattttcg accgtactta taaaagagac ctgctagaag
     8581 ggctccaaaa gtcgctcctg tgagagctaa aggcggaatc ccttgagtcg tcatacggat
     8641 aaaggctgtc actgtagcca tagccaaggc ataaacaggt cccatcatga ttcccgctag
     8701 aatattgact acactggaca tcggtgccat tccctcaatc cgaaagatag gtgtaaggac
     8761 tacatcaagg gcaatcatca tagataaaat ggtcaatttg tgaacttgta gttggtgctt
     8821 tctcatgttt ctattcttct cctttttcta aagactgtaa atcgctcttc catgtctggt
     8881 gttggtaagc catctcccaa aacttggctt ccatatgaac actgatgtgg aaggcatcta
     8941 gcattttttg cttatctgtc tcatcacttt ctcgatagag ctgattgacc agtgctccct
     9001 cctctctgat ctgttgctct aactcatccg taatataagt ttcaatccat tgttgataga
     9061 gaggatttgg tgatggttta agattaagtg atttgcctat atcatggtat aaccaaggac
     9121 aaggaagcaa gcttgcaaaa gcgatggcta agttcggttc tgcaaattgc ctataaatat
     9181 gagaaatgta atgataacag gttggagcga ttggatgttg ctccatttcc tggtcgctga
     9241 tttccaattc cttgaaaaat tgttggcgaa taaataactc accctccact aaaccctgag
     9301 cattttgttt caagagtctt ttcatctctt ggtttgaagt cttatcagcc aaaagatgat
     9361 agatttctga gaaagccttc agatagtagg catcctgaat caggtaatag cggaaaatgg
     9421 caggttctaa attcccctct tgtaattgta aaataaaggg atgatgaaag gaagcctgcc
     9481 aagctttctt ggataattcc atcgcaatat ctgtaaattc cataataact cctttataaa
     9541 aatagactgg tttgaagcaa taaaaagaaa agcaggtaga ttaattttgt ttttttagga
     9601 atataaaaag tccgatagct attcttcaac tgtgcatgtt cgtcatatcc gtgagcagat
     9661 agagctctca ggtaaagatg gcgccaccta aagactgtca tcagaacctt actgtaaatc
     9721 aagggcgacc aaaaatgtag ttcttgacca cgtaataggc aagcttcttt gagggacttg
     9781 atttcttgct gaatgagagg aaaagaattg aataccacaa tcaaggcata ggaccaagag
     9841 cgtgatagcc ccttttgagc caagtacaag agaagctctt ttagtgaaat agaggaaaca
     9901 aagacaaggc cgatacaaac tgtcacaaag gccctcgttc caagcatgac tgcctgtgaa
     9961 gcatctccgt gtaactgaac tgcccagtag ttggcaaaag atggtaaaat ggcaagtatg
    10021 atcatccaag ctaacatttt aaatcgacgg taatagagca taaagagaat acaaaatgcg
    10081 actaccgaaa gattcagagc aatcgaagga atgaaagatg tttccaagga taaaatcagc
    10141 aagaagagac tgataatcgg tgtctgggtt gctactttga ccatactatc tcacctcccc
    10201 ttgggtattg ctactctgag atgtaagtgg tttggtaatg gtcacttctt tcacatgccg
    10261 aagaccctga ctagtcatct caatccaata atcaaccaca gaaatcaaag ggtctaaacg
    10321 atgactaatg agcagaaaac ttcttccttg attcctctcc tccacaatcc acttgcaaaa
    10381 ataatggcag gctctatcat ccaaacctgc aaaaggttca tctagcaaga tcacggaagc
    10441 cttactggtc aagatggtca ggagctgaag aattttttgc tgaccaccac ttaattgata
    10501 gggactctta tcgactgcct gctccaaatc aaaatatcgt aaagcttgaa aaatccgctg
    10561 atttctttca gaatcaggtc catctaattg aagctcctct cgcagactga ctcggataaa
    10621 ctgcttctca gcttcctgaa caacaccagt cagatcacga tacaaactct ttttcttttt
    10681 caggaccgaa cccttccatg caatgcgccc cttatacttt tgaaattgaa gaatagaccg
    10741 aaagagggtt gatttcccga caccattgtc acccaggata caggaaatcc cttgatagaa
    10801 tgtgaaatca gcaattgaaa agagggggcg atttccaaac tcacaagtcg ttctatccat
    10861 atggaatagt tccgggctag aagcaacttc ctttgaagca acctgtgtca tctcatagga
    10921 agggatttga aacacttccc ttagttttcc gtctcttagc tcaaccatat ggtcgatata
    10981 ggctttatag tcagataaat catggtcgca caaaataact gtctttccat caagagccaa
    11041 ctcttttaga atctccaata tctcgattct gctcttgcgg tcaatggaag caaaaggttc
    11101 atccaagaga tagaccctag gattcatggc aaagaggaca gccagcgctg ctttttgctt
    11161 ttccccacct gataggtgat gaatgggacg gtgtaagatt gcctcgcaac gacattgctg
    11221 gacaacctct gctattttag aatcaatttc ctgaaggtga tagccgatat tctccaaggt
    11281 gaaaatcagc tcctcaaaca agctctccat ggtaaattga tgattgggat tttgaaagag
    11341 aatgccaacc ttctggacac gtgcgatgat agaaagttga ctgacctcgc tcccatctat
    11401 caggacttga ccactatagg gaagagaact gacttgggca atcatttgaa agaggctgga
    11461 ttttccagac ccactactcc caactaacaa ggtaaaggct tgcgcatgaa aagtaaaatc
    11521 aaacggctca gagaagattg gggactgaat cgctcgtagt tccagaccca tctatgcttt
    11581 tcctccagtt gcaaactgat gatagagttt gacaatggca cgaaccaaga tggtacagaa
    11641 gaaataaaca gaaataaaac gtaccacaag taaggaaagg acaaacggaa gggaaaaggc
    11701 gtagtaacct aacttaatgt attcatagac aaagctaaca agcgtaatcc caatactatt
    11761 agcagttaga gagagccaac tttcatagcg attcttagtt acgataaaac caaattcact
    11821 tcccaaacct tgaaccaagc cagacaaaag agctcctaga ccaaattggc taccataaag
    11881 gacttcagca agcgcagcta gcacttctcc aatcgttgca cttccgactc tcggaacaaa
    11941 gatggcagca atgggcgcag ccatacacca gagaccgaag aggatttcat tggcaaaggc
    12001 ctgcaaacca agaggtgtta agagtagact gagaatatta tacacatatc ctgaaccaac
    12061 gaaaacccca ccaaaaaaga tagacaagaa agcaagcaag ataacatctt ttaactgcca
    12121 ttttttcaac ataaaaaact ccttttttta aagaaaagtg gggcactcaa gaagaccgac
    12181 ctaaatactt tgtatagcag actgaattta gaacagtaca caagaatgct aaaatatttc
    12241 tagaaattaa tttgaatttt ctaattgatt tgttcgcatc ttatttcaat ctactatatc
    12301 atcttcatcc agtttcgtaa aagaaaaaac tctaattaca gatacgaatt agagttcagc
    12361 ttacaagatt agacagttct tttcgacata cgaaaaaaac atttcacatt tcccttcgcc
    12421 agtcttaa
//
LOCUS       DAVLVF010000047        12268 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVLVF010000047 DAVLVF010000000
VERSION     DAVLVF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12268
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.047"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(4..201)
                     /gene="blpI"
                     /locus_tag="VYN66_001322"
     CDS             complement(4..201)
                     /gene="blpI"
                     /locus_tag="VYN66_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEV1495337.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            483..1070
                     /locus_tag="VYN66_001323"
     CDS             483..1070
                     /locus_tag="VYN66_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV1495338.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            997..2640
                     /locus_tag="VYN66_001324"
     CDS             997..2640
                     /locus_tag="VYN66_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1495339.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2651..4012
                     /locus_tag="VYN66_001325"
     CDS             2651..4012
                     /locus_tag="VYN66_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV1495340.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            4069..4197
                     /gene="blpC"
                     /locus_tag="VYN66_001326"
     CDS             4069..4197
                     /gene="blpC"
                     /locus_tag="VYN66_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV1495341.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(4268..5608)
                     /locus_tag="VYN66_001327"
     CDS             complement(4268..5608)
                     /locus_tag="VYN66_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1495342.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(5622..6359)
                     /locus_tag="VYN66_001328"
     CDS             complement(5622..6359)
                     /locus_tag="VYN66_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1495343.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(6364..6702)
                     /locus_tag="VYN66_001329"
     CDS             complement(6364..6702)
                     /locus_tag="VYN66_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1495344.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            6978..7307
                     /locus_tag="VYN66_001330"
     CDS             6978..7307
                     /locus_tag="VYN66_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1495345.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7475..8524)
                     /locus_tag="VYN66_001331"
     CDS             complement(7475..8524)
                     /locus_tag="VYN66_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1495346.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVSGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8521..9252)
                     /locus_tag="VYN66_001332"
     CDS             complement(8521..9252)
                     /locus_tag="VYN66_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495347.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9320..9730
                     /locus_tag="VYN66_001333"
     CDS             9320..9730
                     /locus_tag="VYN66_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1495348.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9740..10027
                     /locus_tag="VYN66_001334"
     CDS             9740..10027
                     /locus_tag="VYN66_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495349.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            10282..10473
                     /locus_tag="VYN66_001335"
     CDS             10282..10473
                     /locus_tag="VYN66_001335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495350.1"
                     /translation="MDRTDEVSSKHCFEVVDTTDEVSSKHCFEVVDRTDEVSNHTHDK
                     PTLTWFEEIFEEYHSPFHN"
     gene            complement(10785..11921)
                     /gene="dnaJ"
                     /locus_tag="VYN66_001336"
     CDS             complement(10785..11921)
                     /gene="dnaJ"
                     /locus_tag="VYN66_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV1495351.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
BASE COUNT         3955 a         2654 c         2179 g         3480 t
ORIGIN      
        1 atcttacaaa gaggacataa ttcctgaacc tacacaaata agtgttgctg ctcccccagt
       61 tatcggacca gtcgcagcag ctaatagtac tgctccaata caaccaccga ttgcagatcc
      121 taaattgcct cttcctccac taactatttc gagttcttca ttatccataa cagaaaattg
      181 ttccatcatt tttgtattca tgacaaatac tccttttttc tttttttatt tttgtcttgt
      241 tgtaactttg ataagtttag tatatcatcg ttttttaaaa tttttcatcc agatcttgaa
      301 ttgtcatcga aacgtcttga attaggtttt ttatttcaag ccacctctaa atgtttaaaa
      361 aaaataattt ctaatcactt ttttaccatt caggaagttt taatgactat tcaagatttc
      421 ataaaatatg aacttagttt tatgacataa tagacctatc cactatatga aaggaattgc
      481 caatgacttc ttataaacgt acatttgttc ctcaaataga tgcgagagac tgtggtgtcg
      541 ctgccttagc ctcgattgct aaattctatg gttcagattt ttctctagct cacttgagag
      601 aacttgcaaa gaccaataaa gaagggacga ctgctcttgg cattgtaaaa gccgctgatg
      661 aaatgggctt tgaaacaaga cctgttcaag cagataaaac gctctttgac atgagtgatg
      721 tcccctatcc atttatcgtt cacgttaaca aagaaggaaa actccaacat tactatgttg
      781 tctatcaaac aaagaaagac tatctgatta ttggtgatcc tgacccttct gtaaaaatca
      841 ctaaaatgtc aaaagaacgc tttttctatg aatggactgg agtagctatt tttctagcta
      901 ccaaacccag ctatcaaccc cataaagata aaaagaatgg tctactaagc aagcttcctt
      961 cctctgattt tcaaacaaaa atctctcatt gcttacattg ttctctcaag cttattggtc
     1021 actattatca atataggtgg ttcttactat ctccaaggaa tcttggatga atacattcca
     1081 aatcagatga aatcaacttt aggaatcatc tcagttggtc tggttatcac ctatatcctc
     1141 caacaagtca tgagcttctc cagagattat ctcctaaccg ttctgagtca gagattaagt
     1201 attgatgtga ttttatccta tattcgccat atttttgaac ttcccatgtc tttctttgcg
     1261 acacgtcgta caggagaaat catttcacga ttcacagatg ctaactctat tatagatgcc
     1321 ttggcttcta ccattctttc tctttttctg gatgtttcta ttctgattct tgtaggaggc
     1381 gtcttactgg cacaaaaccc taatctcttc cttctttctc ttatttccat tcctatatac
     1441 atgttcatca tcttttcttt tatgaaacct ttcgaaaaaa tgaaccatga tgtcatgcaa
     1501 agtaattcta tggttagctc tgccattatc gaagatatca acgggattga aactataaag
     1561 tcgctcacga gtgaagaaaa tcgctatcaa aatatagaca gcgaatttgt agattatttg
     1621 gaaaaatcct ttaagctcag taaatattct attttacaaa cgagtttaaa gcagggaaca
     1681 aaattagttc tgaatatcct tatcctatgg tttggcgctc aattagtcat gtcgagtaaa
     1741 atttctatcg gtcagctgat tacctttaac acactttttt cttactttac aactcctatg
     1801 gaaaatatta tcaacctcca aaccaaactc caatctgcga aggtcgctaa taaccgtttg
     1861 aacgaagtct atctagtcga atctgaattt caagttcaag aaaaccctgt ccattcacat
     1921 tttttgatgg gcgatattga atttgatgac ctttcttata agtatggttt tggacgagat
     1981 accttaacag atattaatct cacgattaaa caaggagata aggttagcct agttggagtt
     2041 agtggttctg gtaaaacaac tttagccaaa atgattgtca atttctttga accctacaaa
     2101 ggacatattt ctatcaatca tcaggatatt aaaaacattg ataaaaaagt cttgcgccgt
     2161 catattaatt acctaccaca acaagcctat atctttaatg gctctatctt ggaaaactta
     2221 actttgggcg gtaatcatat gattagccaa gaagatattc taagagcttg tgaattagct
     2281 gaaattcgtc aagacattga aagaatgcct atgggctatc aaactcagct ctctgatgga
     2341 gctggtctat caggaggaca aaagcaacga atagccctcg ctcgtgctct tttaactaaa
     2401 gctcctgttt taatactaga cgaagctact agcggtcttg atgtcttgac tgagaaaaag
     2461 gttatagata atcttatgtc tctaactgat aaaaccattc tctttgtagc ccatcgtctc
     2521 agtatagccg aacgaaccaa ccgtgtcatt gttcttgacc aggggaaaat cattgaagtt
     2581 ggtagtcacc aagagttaat gcaggcgcaa ggtttctacc atcatctatt caataaataa
     2641 ggagaatgtc atgaatccta atctttttag aagcgtcgag ttttatcaga gacgttacca
     2701 taactatgcg acagtgttca ttatacctct ttcattacta tttactttca tcttgatttt
     2761 ctcccttgtt gccacaaaag aaattactgt tacttcccaa ggagaaatcg cccctacaag
     2821 tgtcattgcc tccattcagt caaccagtga taatcctatc ctagctaatc atttagtggc
     2881 aaatcaagta gttgaaaaag gggacttact catcaaatac tctgaaacaa tggaagaaag
     2941 tcagaaaact gccttagcaa ctcaattaca aagacttgag aagcaaaaag aaggacttgg
     3001 aattttgaaa caaagcttag aaaaagcgac tgatcttttt tctggcgagg atgaatttgg
     3061 ctaccataat acctttatga attttactaa acaatcccat gatattgaac tgggtatcac
     3121 aaagactaac accgaagttt caaatcaagc taatctttcc aatagcagtt catcagctat
     3181 tgaacaagaa attacaaaag ttcaacaaca aattggagaa tatcaagagt tgagagatgc
     3241 tatcataaat aacagagcac gcttaccaac tggcaatccg caccagtcaa ttttgaatcg
     3301 ttatcttgta gcctcacaag gacaaacaca aggaactgca gaggagccat ttttatctca
     3361 aattaatcaa agtattgcag gtcttgaatc atctatcgca agcctcaaaa ttcagcaagc
     3421 tggtatcgga agtgtagcaa cttatgataa cagtttagca accaaaattg aagtactccg
     3481 cactcagttt ttacagacag cctcacagca acaactaact gtggagaatc aattaacaga
     3541 attaaaagta caactagatc aagccacaca gcgtttggaa aacaatacct taacctcccc
     3601 aagtaaaggt atcgttcatc tgaacagcga atttgaaggt aaaaatagaa ttccaactgg
     3661 tacagaaatt gctcaaatat tccctgtcat cacagataca agagaagtac taatcactta
     3721 ctacgtatct tctgactatc tacctctact agataaagga caaactgtaa gattaaaact
     3781 ggagaagatt ggaaatcacg gcaccaccat catcggccaa cttcagacaa ttgatcaaac
     3841 tcctaccaga acagagcaag gaaatctctt taaattaacc gctcttgcaa aactatctaa
     3901 cgaggatagt aaactcatcc aatatggctt acaaggtcgc gtcactagtg taactacaaa
     3961 gaaaacatat tttgattatt tcaaagataa aattttaacc cattctgatt aattttcaga
     4021 taacactcta taactattta ttatcttatc aaaaaggaga atcataacat ggataagaaa
     4081 caaaacctaa cttcatttca agaactaaca actaccgaac tcaaccaaat tacaggtgga
     4141 ggattgtggg aagatttatt atataacatt aatagatatg ctcattacat cacataagaa
     4201 cttcatcatc caatacaact ataaaaaaat aagaccgaga aacaagtact ctcggtctta
     4261 tttttcatca ttctgtatgt atcacagtaa gtacctgacg aaagacttga ttttgacagg
     4321 tggtatttag actggtatta ggatggcttt ccacaatctt catgacggta tagagaccaa
     4381 ctcctctctc ctccccttta gaactggctc caaaggagaa gatttcagaa atatcgatgc
     4441 cctcttcttt gatggagttt tcgatgataa aggtctcctg tgctccattt tttaaaaagg
     4501 cgattgaaac atgaggttga ctagcttcca cactggcttc aatagcattg tcacaaagga
     4561 tagacacaat ggttagaaaa tcaagtagac tcatcccctc gacctgaatc tcctcaggaa
     4621 cttcgacatt aaagacaatg ttcttatctc tggcttttaa aaatttccct gctagaagac
     4681 ttttgagggc tttatcacga atatttacca atctgcccag gtcatattta ttgttctgca
     4741 atttctgact ggaatccttt aagacggagc catagacctc ttttatctgc tccatatcct
     4801 cctcttcaat gcccagacgt aagctagtca agaggttggt ataatcatga cgaaagctcc
     4861 gtacttcctt gtaaagctcc tctatatgcc gactatagcg ttccatatct ctatagcgca
     4921 gggcctgctc ttgttccaat ctctcataga gtttttcctt caaataggta tccaatttct
     4981 tgataacccc cataaaaaag agtaggtaaa agactaggat gagatggcga acagtctttg
     5041 attgaatact ttgttcatat tcaaaaaaag acagactttc catgactaga tagtagccac
     5101 ccattatcca gttaatctga gtcagggact tttgaaaggc tttatcgaga atctcctttc
     5161 tcaagctagt aaaatcgtag tccaaccatt tcaaaaaagc tagagaaatg aagaaattga
     5221 aaattattat acataaccca gtaaatgagt agccatcata tacttgccct tgtcccaaaa
     5281 atggaagcac aaaataggag actcctctat aaaagagatt caccaatatc attggaaaga
     5341 gaccataaaa gaaaaggagt tttttaggaa gccctctcaa taataagaaa gataagccta
     5401 tgccgtacaa gggttccata aaataagata ggtaaacatt tcctactata tagctaatca
     5461 tcacaaaaac aaaggccaac agtatcttaa aaagaaaggc cttaaaaatc ctctcgaaag
     5521 taagatcaat tccatccacc ttaaagaaga tgacaatttc tagtccatta gtaacaagtg
     5581 tatacaacaa tatccaagca atgttcataa attctcctag ctcagtgtaa tttattgatg
     5641 gcctcagaca cttccctgac cttataacgg gcgattagac aacttccacc attgggaaag
     5701 aaaagcagtt tttctttctt atccaaatgc accacatttg caggattgat gagaaaagag
     5761 cggtggcact gcaagagacg gggctcctgc ttgaaaacct cctctaaact cgctgtaaat
     5821 tccagcctgt ctgtcttggt atagagaata acacgatggg ctctgggcga cgtttcgaga
     5881 tagtaaacct ctttaaaagg atactgaaat tgggcaaatt ttgatttaaa gtaaaagcaa
     5941 tcttccgcca gacttttact atcttgacta ttggcataga ggagggctgt ctcgatccga
     6001 gattcaaact cctctgctga caaggcctta tcaatgtagt ccaaagcaga cacttggtag
     6061 cgaaaagaca ggggcataaa ctccgagtga gtcgtcacaa agacaatcag ggcataagga
     6121 tcccgatccc gaatctttct agccacttcc agtcccttca tctcttcatt tcgaatctca
     6181 atatccaaaa agaatagctg atgggccccc ttctcatgca cttcagccag cagttggtcc
     6241 ggcttgccaa atacctcaaa agagctagga atgatatgat gtgctttcaa aagtttctca
     6301 atcgtcgttt caattctagt ctgttgggaa aaatcatcct ctaaaacaaa tattctcatc
     6361 ttcttactct ccctctttca accatttttg acgaatttct ctatagcgac gtctggcata
     6421 cttgacagcg tattgacctt cttctccgag aaaaaggatt ctttcttgaa aatcaatcga
     6481 ttttaattta tcaagattaa ccaaacaatt tcgatgacat ctcatcaagg tttccccaca
     6541 ttggttctca agattactta aattttgaag catattaaaa ctagcttctt ctgtaacaat
     6601 ctgtacggta tgggctttag ttggatgtgt ttggatatag tagatatcgt cgatgtttac
     6661 tttatagact gttttctgcg tctgaataat catatatttc attggattct cctcctaaga
     6721 aaagaataac agaaaaggtc tagcccaagc acacccaatc ctaaacagtc tttgaaaact
     6781 cctaaaagga ttattttcta caacatcatg atgagcttac aactatttta tcataaaatt
     6841 cactcagcgc aattcaagac atttcaatga caattaaaga tttgaataaa aaaaaagaat
     6901 taaaagtgat aaactaacag tatcaaaaca cagttgccag aagtaagact ggcacccaga
     6961 ttaaaggagg cattcccatg acagatacag accccatcaa aagagctcat actttgatta
     7021 ctgacttaaa caaagcctac caagcatgca aacaggcaag cgctgacgac gtccgctttc
     7081 aggagcaatt aaactctatt cttggttttc tagccaaggc tgaaacagtg gataatcgat
     7141 tcttgattga actagaaaaa ttttaccaga cttccagtct tctcatggga ctcagcgctc
     7201 ttgatccaga tgctccaact cgcgccgctt ggcgggccta tgaccgcttc cactttgacc
     7261 aagtcaagac caagttaata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca
     7321 agctgtactt gagtacggta agacgacgct gacgtggttt gaagagattt tcgaagagta
     7381 taagtctcta cggaccaacc attatcctgt agtaacaaaa aagaagttga gacagactga
     7441 actcaacttc ttttttagtg tcgtatcagc aatgttagtc ctgcatctga cgttttacct
     7501 gataaggcaa atacaagact agtaaaacca aaccagctcc tagtaaggct agaagggcta
     7561 gtttttcttg gaaggtaatc aacccaacaa ctaattccac aagtaaaaca aaactggtca
     7621 atcctcgaac tacctcctgt aagccttttt ccttttgccc cttgtccagc ggaaagagtt
     7681 gggtcaaata ctggtagtca aaggcatgat agagggccag caactggaag agcaagaggt
     7741 agttaaagag aactaccact gctgtcgcaa tccaagcttg ctcgataaaa acctgcgcca
     7801 gtacggaaag taagagaaga cgaagactga gagcaaagag gtcgccattt cgcagataag
     7861 aacgcagata gagattttgc caaatctttc cagacacctt ctgaacagcc tttaagataa
     7921 agtccagata ggcacgacgt ttgacactgt ttgaaattcc ctttacctgc gtaaagaggg
     7981 caaagaaacg aagcaagact tgcttacgct tgctttcttg ggaaatgaca tagtcccagt
     8041 ccagcccagt ttcagtgaaa aatttgctgg ccttttgacg aaagtggaaa tattttccta
     8101 cccccaataa aagcacatag agcagaaaaa ctggcaagcc ataacccatt gctaaaaata
     8161 aaggcgcaaa taacagcaag aaaagggtct gtacaaagag ccaaaagact agggaaatgc
     8221 cagtttgacg cttgagatgg agcttgattt cctcttctcc aactaagaga aagagcttgt
     8281 ctggagcctc catataggtg gcaattcctc cccaaagtaa aagtaaaaca gacgtaattc
     8341 ctacaaacaa aaggataggc caatgatttt caggaaaatg ttgtaagagt tgactgtact
     8401 ggtaggctag aaagcccaac aggacaagca ggaacaagac aaagtggtcg ttgagcacat
     8461 agcgcagata accaaggcac tccttacgaa aggcctgctt tctctttaaa aacaagtctt
     8521 tcatagatcc tcctctttgg tcagagccaa gtaaatatca ttcaaactag cctcaggcat
     8581 atcaaaggct tcacgtagtt gcaggagatt gcctttggca cgcacctctc ccttgtgaag
     8641 aatgacaaag gcatcacaca tcttctccgc cgaatccagc acgtgggtac tcatgagaat
     8701 agacttgccc ttttgcttct ccacttccaa aagctgaatc aaatcagaaa tagccagcgg
     8761 atcaagacca aggaaaggct catccacgat gaaaagactt ggatccacca caaaagcaca
     8821 gataatcatg accttctgct tcatcccttt tgaaaaatga acagggaacc agtctaattt
     8881 ctgttccaaa cggaacattt ttaacaaggg ctctactcgt tcgaaagcca ctttttgctc
     8941 aataccgtaa gccatagcaa ccgtttcgat atgctctctg agggtcaatt cctcatacag
     9001 actaggcgtc tcaggaatgt agccaatctg cttgcggtag ctagtcgcat ctccttgcag
     9061 agtcaggcca ttgatattga tggagccact ataaggtgcc aacagaccga taatctcatt
     9121 gatcgtcgtt gatttcccag caccattgag accaatcaaa ccgaccaact gcccactttc
     9181 aacagtaaag gacacatctt tcaaaacagg gacatgaaca tagccacctg tcaggttttt
     9241 aatttctaac atattttctc caaatctggt ataatgtagc tatattatat caaaattcag
     9301 tacagtagag gtagatttta tgtcagattg cattttttgt aaaatcatcg caggggaaat
     9361 tcctgcttca aaagtatatg aagatgagca ggtccttgcc tttcttgata tctctcaagt
     9421 aacactagga cacaccttgg tcgtgccaaa agaacactat cgcaatcttt tggagatgga
     9481 tgctacgagc gccagccaac tctttgccca agtaccaaaa gtagctcaaa aagtcatgaa
     9541 agtcactaag gctgctggta tgaatatcat ttccaactgt gaagaagtcg ctggtcaaac
     9601 agtttttcat actcacgttc accttgtacc tcgctacagt gctgacgatg acctcaagat
     9661 tgattttatc gcccacgaac cagactttga caaacttgct caagtcgctg aaaccatcaa
     9721 aaacgcttaa ggagttgcta tgaaattatc caacctactg ctatttgcag gagctgcagc
     9781 cggaagttat ctggtcacaa aaaatcgcca aaccatcaca gatgaagtct tgaataccac
     9841 tgaccgcgtt caagctatca aggacgatgt ggatattatc caaaacagcc tgcaaatcat
     9901 tgaccagcaa aaagaactta tcaaggaata ccaagaagac ttgacttaca agtttaaggt
     9961 cttggaaaag gatatccaga ctagactagc tgtgataaaa gaaatgcagg gaactgaaga
    10021 taagtaaaaa gagccccccg gctctttttt aaaacgtaaa caagttatag tgaattgaat
    10081 ttagaatagc acatcatagt ttctaaagca ttgttagaga ttactttaaa atcctcttat
    10141 caatttgttc atattctatt tcaatctact atatatatcc catcaactat gcatcataat
    10201 ttaggtaact catactcaat aaaaatcaaa aagcaaacta gaaagctagg cacagactgc
    10261 tcaaaacacc gttttaaggt tgtggataga actgacgaag tcagctcaaa acactgtttt
    10321 gaggttgtag atacgactga cgaagtcagc tcaaaacact gttttgaggt tgtggataga
    10381 actgacgaag tcagtaacca tacccatgac aagccgacgc tgacgtggtt tgaagaaatt
    10441 tttgaagagt atcattcacc atttcacaac taaatcgaag actttctgcc gtaatgaaaa
    10501 cacctgaaac agcttggttt cggctgtccg gaaactttaa gacttaggtt caaagtttag
    10561 gtatggaact tcgaaggagg tcgctaccgt ccgtcattac ttaggaaagt cttaaaaaat
    10621 cggctctata aagtaaaaag agcccgacag ctctttttgt ttatattcaa tgaaaatcaa
    10681 aaagcaaact aggaaactag ccacgggctg ctcaaaacac tgttttgagg ttgtagatac
    10741 gacaagccga cgctgacgtg gtttgaagaa atttttgaag agtattattc tccatcaaag
    10801 gcatctttaa tatggtcaaa gaagcctttt ttctttggat ttactttcaa gtcaccagca
    10861 gccgcgaatt ctttcaaggc tactttttgg cggtcgttca agcctgtcgg tgttacgaca
    10921 ttaacagtaa cgtattggtc accaactgca ccgccacgaa ggctcggtgc ccccttacta
    10981 cgtaggcgga atttcttacc agtctgagtt ccctctggaa taaccaattc aacatcaccg
    11041 tgaacagttg gaatatctac tgtatcacca agagccgctt ggacaaagtt gaggttgaga
    11101 ttgtagaaga tagtcgttcc ttcacgttca aacttgtcgc tagcttctac agaaactact
    11161 acatacaagt caccataagg tccaccgtta aagcctgctt caccttgacc agcgaggcga
    11221 atttgttgac ctgtttccac accagcaggg attttcacat gtacgctatg agcttgtttc
    11281 tcatgacctg ttccatgaca ggttgtacat ggatatttga tttcttttcc tcgaccgtga
    11341 cagacatcac aggttacttg gcgacgcatc ataccaagag gagtctgcgt atcgacgtta
    11401 atgacaccag cgccatgaca gcgtccacaa gtgactggac ttgtccctgg cttagcacca
    11461 gagccattac atgtacgaca gccagcttca cgatgatact taacttcctt ctcagttccg
    11521 aagatagctt cttcaaaggt caaattgaca cgatactgga gatcatctcc ttggcgagga
    11581 gcgtttggat tgcgcgaaga accgcctccg ccgaagaaac ttgagaaaat atcctcaaaa
    11641 ccaccgaaac cacctgcccc attgaaaccg ccgaaaccac cagctccacc aaaaccacca
    11701 ttggcgcctg cagcaccata ctggtcatag gcagcacgtt tttggtcgtc actcaaagtc
    11761 tcataggctt cttgaacttc cttgtacttg tcctcagcac caggctcctt gttgatatct
    11821 gggtgatatt ttttggaaag cttacgataa gcctttttga tttcgtctgc cgaagcgttt
    11881 ttggataccc ccagacgatc ataaaattca gtattgttca taattcaaga tacaaagggc
    11941 gtcaaacgga cacagccatc taggagtttt gacgacggac gcttacgtcc tagaaaaaac
    12001 tatcttttgg gcacagtccg tagcccgtgt tcagttacga acacccttat tcctttctat
    12061 tgatgttctg aatcgaacca ccatttaggt cgggttcaat tcctttcttt tatatttagt
    12121 aagaaattct gaaacccgtg ttcagttacg aacactcttt gttcccttct attgatgaga
    12181 caaaacccaa acaaatgaga ttccgtccct ttttatcgaa aaacagaggc tgggttgcaa
    12241 cctctgcaaa taagtaaatt aactttat
//
LOCUS       DAVLVF010000048        12061 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVLVF010000048 DAVLVF010000000
VERSION     DAVLVF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12061
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.048"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            16..462
                     /gene="argR"
                     /locus_tag="VYN66_001337"
     CDS             16..462
                     /gene="argR"
                     /locus_tag="VYN66_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1495352.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            513..3047
                     /gene="mutS"
                     /locus_tag="VYN66_001338"
     CDS             513..3047
                     /gene="mutS"
                     /locus_tag="VYN66_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV1495353.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            3194..4888
                     /gene="patA"
                     /locus_tag="VYN66_001339"
     CDS             3194..4888
                     /gene="patA"
                     /locus_tag="VYN66_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV1495354.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLAG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(5218..5481)
                     /locus_tag="VYN66_001340"
                     /pseudo
     CDS             complement(5218..5481)
                     /locus_tag="VYN66_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            5679..7445
                     /gene="patB"
                     /locus_tag="VYN66_001341"
     CDS             5679..7445
                     /gene="patB"
                     /locus_tag="VYN66_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV1495355.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            7584..8273
                     /locus_tag="VYN66_001342"
     CDS             7584..8273
                     /locus_tag="VYN66_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1495356.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            8277..8456
                     /locus_tag="VYN66_001343"
     CDS             8277..8456
                     /locus_tag="VYN66_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495357.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            8443..9792
                     /locus_tag="VYN66_001344"
     CDS             8443..9792
                     /locus_tag="VYN66_001344"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV1495358.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            9916..11376
                     /locus_tag="VYN66_001345"
     CDS             9916..11376
                     /locus_tag="VYN66_001345"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV1495359.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            11390..11821
                     /locus_tag="VYN66_001346"
     CDS             11390..11821
                     /locus_tag="VYN66_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1495360.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNE"
     gene            11945..12016
                     /locus_tag="VYN66_001347"
     tRNA            11945..12016
                     /locus_tag="VYN66_001347"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11978..11980,aa:Glu,seq:ttc)
BASE COUNT         3584 a         2313 c         2750 g         3414 t
ORIGIN      
        1 acgcaagagg attttatgag aaaaagagat cgtcatcagt taataaaaaa aatgattact
       61 gaggagaaat taagtacaca aaaagaaatt caagatcggt tggaggcgca caatgtttgt
      121 gtgacgcaga caaccttgtc tcgtgatttg cgcgaaatcg gcttgaccaa ggtcaagaaa
      181 aatgatatgg tgtattatgt actagtaaat gagacagaaa agattgattt ggtggaattt
      241 ttgtctcatc atttagaagg tgttgcaaga gcagagttta ccttggtgct tcataccaaa
      301 ttgggagaag cctctgtttt ggcaaatatt gtagatgtaa acaaggatga atggatttta
      361 ggaacagttg ctggtgccaa taccttattg gttatttgtc gagatcagca cgttgccaaa
      421 ctcatggaag atcgtttgct agatttgatg aaagataagt aaggtcttgg gagttgctct
      481 caagacttat ttttgaaaag gagagacaga aaatggcgat agaaaagcta tcacccggca
      541 tgcaacagta tgtggatatt aaaaagcaat atccagatgc ttttttgctc tttcggatgg
      601 gtgattttta tgaattattt tatgaggatg cggtcaatgc tgcgcagatt ctggaaattt
      661 ccttaacgag tcgcaacaag aatgccgaca atccgatccc tatggcgggt gttccctatc
      721 attctgccca acagtatatc gatgtcttga ttgagcaggg ttataaggtg gctatcgcag
      781 agcagatgga agatcctaaa caagcagttg gggttgttaa acgagaggtt gttcaggtca
      841 ttacgccagg gacagtggtc gatagcagta agccggacag tcagaataat tttttggttt
      901 ccatagaccg cgaaggcaat caatttggcc tagcttatat ggatttggtg acgggtgact
      961 tttatgtgac aggtcttttg gatttcacgc tggtttgtgg ggaaatccgt aacctcaagg
     1021 ctcgagaagt ggtgttgggt tatgacttgt ctgaggaaga agaacaaatc ctcagccgcc
     1081 agatgaatct ggtactctct tatgaaaaag aaagctttga agaccttcat ttattggatt
     1141 tgcgattggc aacggtggag caaacggcat ctagtaagct gctccagtat gttcatcgga
     1201 ctcagatgag ggaattgaac cacctcaaac ctgttatccg ctacgaaatt aaggatttct
     1261 tgcagatgga ttatgcgacc aaggctagtc tggatttggt tgagaatgct cgctcaggta
     1321 agaaacaagg cagtcttttc tggcttttgg atgaaaccaa aacggctatg gggatgcgtc
     1381 tcttgcgttc ttggattcat cgccccttga ttgataagga acgaatcgtc caacgtcaag
     1441 aagtagtgca ggtctttctc gaccatttct ttgagcgtag tgacttgaca gacagtctca
     1501 agggtgttta tgacattgag cgcttggcta gtcgtgtttc ttttggtaaa accaatccaa
     1561 aggatctctt gcagttggcg actaccttgt ctagtgtgcc acggattcgt gcgattttag
     1621 aagggatgga gcaacctact ctagcctatc tcatcgcaca actggatgca atccctgagt
     1681 tggagagttt gattagcgca gcgattgctc ctgaagctcc tcatgtgatt acagatgggg
     1741 gaattatccg gactggattt gatgagactt tagacaagta tcgttgcgtt ctcagagaag
     1801 ggactagctg gattgctgag attgaggcta aggagcgaga aaactctggt atcagcacgc
     1861 tcaagattga ctacaataaa aaggatggct actattttca tgtgaccaat tcgcaactgg
     1921 gaaatgtgcc agcccacttt ttccgcaagg cgacgctgaa aaactcagaa cgctttggaa
     1981 ccgaagaatt agcccgtatc gagggagata tgcttgaggc gcgtgagaag tcagccaacc
     2041 tcgaatacga aatatttatg cgcattcgtg aagaggtcgg caagtacatc cagcgtttac
     2101 aagctctagc ccaaggaatt gcgacggttg atgtcttaca gagtctggcg gttgtggctg
     2161 aaacccagca tttgattcga cctgagtttg gtgacgattc acaaattgat atccggaaag
     2221 ggcgccatgc tgtcgttgaa aaggttatgg gggctcagac ctatattcca aatacgattc
     2281 agatggcaga agataccagt attcaactgg ttacagggcc aaacatgagt gggaagtcta
     2341 cctatatgcg tcagttagcc atgacggcgg ttatggccca gctgggttcc tatgttcctg
     2401 ctgaaagcgc ccatttaccg atttttgatg cgatttttac ccgtatcgga gcagcagatg
     2461 acttggtttc gggtcagtca acctttatgg tggagatgat ggaggccaat aatgccattt
     2521 cgcatgcgac caagaactct ctcattctct ttgatgaatt gggacgtgga actgcaactt
     2581 atgacgggat ggctcttgct cagtccatca tcgaatatat ccatgagcac atcggagcta
     2641 agaccctctt tgcgacccac taccatgagt tgactagtct ggagtctagt ttacaacact
     2701 tggtcaatgt ccacgtggca actttggagc aggatgggca ggtcaccttc cttcacaaga
     2761 ttgaaccggg accagctgat aaatcctacg gtatccatgt tgccaagatt gctggcttgc
     2821 cagcagacct tttagcaagg gcggataaga ttttgactca gctagagaat caaggaacag
     2881 agagtcctcc tcccatgaga caaactagtg ctgtcactga acagatttca ctctttgata
     2941 gggcagaaga gcatcctatc ctagcagaat tagctaaact ggatgtgtat aatatgacac
     3001 ctatgcaggt tatgaatgtc ttagtagagt taaaacagaa actataaaac caagactcac
     3061 tagttaatct agctgtatca aggagacttc tttgacaatt ctccactttt ttgctagaat
     3121 aacatcacac aaacagaatg aaaaggagct gacgcattgt cgctcccttt tgtctatttt
     3181 ttaaggagaa agtatgctga ttcagaaaat aaaaacctac aagtggcagg ccctggcttc
     3241 gctcctgatg acaggcttga tggttgctag ttcacttctg caaccgcgtt atctgcagga
     3301 agtcttaggc gccctccttg ctgggaaata tgaagctatt tatagtatcg gggcttggtt
     3361 gattggtgtg gccgtagtcg gtctagttgc tggtggactc aatgttgtcc tcgcagccta
     3421 tattgcccaa ggagtttcat ccgaccttcg ggaggatgcc ttccgtaaaa ttcaaacctt
     3481 ttcttatgct gatattgaac aatttaatgc gggaaatcta gtcgttcgaa tgacaaatga
     3541 tatcaaccag attcagaacg ttgtcatgat gaccttccag attcttttca gacttcccct
     3601 cttgttcatc ggttcgttta tcctagcggt tcaaacctta ccttctctgt ggtgggtgat
     3661 tgttctcatg gtagtcttga tttttggttt gactgctgtc atgatgggaa tgatggggcc
     3721 tcgttttgcc aagtttcaaa cccttcttga gcgcatcaat gccattgcca aggaaaattt
     3781 acgtggcgtt cgtgtggtca agtcctttgt ccaagaaaaa gagcaatttg ctaagtttac
     3841 agaggtctca gacgagcttc ttggtcaaaa cctttacatt ggttatgcct tttcagtagt
     3901 ggaacccttt atgatgttgg ttggttacgg ggcggtcttc ctctctattt ggctggtcgc
     3961 gggaatggtt cagtcggatc cgtctgttgt tggttccatc gcttcttttg ttaattacct
     4021 aagccagatt atctttacca ttgttatggt tggatttttg ggaaattctg tcagccgtgc
     4081 catgatttcc atgcgtcgta ttcgagaaat tcttgacgca gagccagcta tgaccttcaa
     4141 ggatatccca gatgaagagt tggttggaag ccttagcttt gaaaatgtga cctttaccta
     4201 tccaatggac aaggaaccga tgctgaaaga tgtgagcttt actattgaac ctggtcaaat
     4261 ggttggtgta gttggagcga ctggtgcagg aaagtcaacc ttggctcaat tgattccacg
     4321 tctctttgat ccacaggaag gggctatcaa aatcggaggt aaggatattc gagaagtgag
     4381 tgaaggaacc ctgcgtaaaa cagtttccat cgttctccaa cgtgccattc tttttagtgg
     4441 aacgattgca gataacttga gacaggggaa gggaaatgct actctatttg aaatggagcg
     4501 cgcagccaat attgcccagg ctagtgaatt cattcatcgt atggagaaaa cctttgaaag
     4561 tccagttgaa gaacggggaa ccaatttctc tggtggacaa aaacaaagga tgtcgattgc
     4621 gcgtgggatt gtcagcaatc cacgtattct gatttttgat gattcgacct cagccttgga
     4681 tgccaaatca gagcgcttgg tgcaagaagc tttgaataag gacttgaagg ggacgacaac
     4741 cattattatt gctcaaaaaa ttagctcggt tgtccatgca gacaagatct tggttctaaa
     4801 tcaaggacga ttgattggtc aaggcacgca tgcagacttg gttgccaaca atgccgttta
     4861 ccgtgaaatc tatgaaacac agaaatgaaa gacaaactat aagaaaagtc aatagtttta
     4921 tctaaactat ttcttatttc aatttgatga tttggcgatg attttagagc acggcaaaaa
     4981 gctattgaaa aagtccattt tttcaaaggt aatcctgtgt taatttcaga aattacatca
     5041 ctttttgttc gtcaaatggc agctcttttt taggatataa aacagggttc ggataagttt
     5101 ttttgcaagg tggatgatgg ctacattgta atgttttcct tgttctaact tagtcttaag
     5161 ataggcctta aaagcaggtg aaaagcgagg gcatgctttg gcagcttgta tgagtaccta
     5221 ccgcagatga ggggaacccc gtttgaccat tctttctgct aaatcaatct gacctgactg
     5281 ataaatagaa gaatccagtc cagcgaaagc ttgtaattga gcaggattat caaaggcatg
     5341 aatatttcga atctcggcta aaatgaccgc ccctaaacga cccccaatcc cagtaaccgt
     5401 cgtgatgacc gagttgaact cagccatcaa gtcattgaca cattttttcg ccttgtcaat
     5461 gagcctcttg taatgtttga tgttttcatt acaagagata aaacgtttat gcgttatcaa
     5521 actcattacc aattgaaaca aaaagctgtg gttagatcct ttcggaaatt gtcaagcgat
     5581 tggaggaaat gaactaatcc acagcggctt attccaagta taccacttgg gctttggcag
     5641 tagttaactg cgctaaatat aatataagga ggagtaaaat gaagacagtt caattttttt
     5701 ggcattattt taaggtctac aagttctcat ttgtagttgt catcctgatg attgttctgg
     5761 cgacttttgc ccaagccctc tttccagtct tttctggaca agcggtgacg cagctagcca
     5821 atttagttca agcttatcaa aatggcaatc cagaacttgt atggcaaagc ctatcaggaa
     5881 tcatggtcaa tcttggcctg ctggttttgg ttctatttat ctctagtgta atatacatgt
     5941 gtctcatgac gcgcgtgatt gcagaatcga ccaacgagat gcgcaaaggc ctctttggta
     6001 agcttgctca gttgacggtt tctttctttg accgtcgaca agatggcgat atcctgtctc
     6061 attttaccag tgatttggat aatatcctcc aagcctttaa cgaaagcttg attcaggtca
     6121 tgagcaatat tgttttatac attggtctga ttcttgtcat gttttcgaga aatgtgacgc
     6181 tggctctcat caccattgcc agcactccat tggctttcct tatgctgatt ttcatcgtga
     6241 aaatggcacg taaatacacc aacctccagc agaaagaggt agggaagctc aacgcctata
     6301 tggatgagag catctcaggc caaaaagccg tgattgtgca aggaattcaa gaggatatga
     6361 tggcaggatt tcttgaacaa aatgagcgcg tgcgcaaggc aacctttaaa ggaagaatgt
     6421 tctcaggaat tcttttccct gtcatgaatg ggatgagcct gattaataca gccatcgtca
     6481 tctttgctgg ttcggctgta cttttgaatg ataagtctat tgaaacaagt acagccctag
     6541 gtttgattgt tatgtttgca caattttcac agcagtacta ccagcctatt atccaagttg
     6601 cagcgagttg gggaagcctt cagttggcct ttactggagc tgaacgaatt caggaaatgt
     6661 ttgatgcaga ggaggaaatc cgacctgaaa aggctccaac cttcactaag ttgcaagaaa
     6721 gtgttgaaat cagtcatatc gatttttcat acttgcctga taaacctatt ttgaaagatg
     6781 tcagcatttc tgcccctaaa ggccagatga cagcagttgt tgggcctaca ggttcaggaa
     6841 aaacgactat tatgaacctc atcaatcgct tttatgatgt tgatgctggt ggtatttatt
     6901 ttgatggtaa agacattcgt ggctatgact tagatagtct tagaagcaag gtgggaattg
     6961 tattgcaaga ttcggtcttg tttagcggaa cgattcgaga caatatccga tttggtgtgc
     7021 cagatgctaa tcaggaaatg gttgaggtag cagcaaaagc aacccacatt cacgactata
     7081 tcgaaagttt gcctgataag tacgatactc ttattgatga tgaccagagc atcttttcaa
     7141 cagggcagaa gcaattgatt tcaatcgctc gaaccctgat gacagatcca gaagttctca
     7201 ttctcgatga agcaacttca aacgtagata cggtgacgga aagcaagatt cagcatgcca
     7261 tggaggtggt tgtagcaggt agaactagtt tcgtcattgc ccaccgcttg aaaaccattc
     7321 tcaatgcaga tcagattatt gtccttaaag atggagaagt cattgaacgt ggtaaccacc
     7381 atgaactttt gaagctaggt ggcttttatt cagaactcta tcacaatcaa tttgttttcg
     7441 aataagaaag aagttgtcct atgtgggcag ctttttcttg tccataaaaa atgtttatca
     7501 cagccttaaa aaaaacatat tagacgaaag tcattttgag tgatatgata ggactatcgt
     7561 tagtattcga aaggagaggc atcatggcta gaacggttgt aggagttgct gcaaatctat
     7621 gtcccgtaga cgcagaaggc aaaatcattc attcatctgt atcttgtaga ttcgcagaga
     7681 tcattcgtca agtcggtggt ctccctttag tcattcctgt tggtgatgag tcagttgtac
     7741 gtgattatgt ggaaatgatt gacaaactca ttttgacagg aggccaaaat gttcatcctc
     7801 agttttatgg agagaaaaag accgtcgaaa gcgatgatta caatctggtc cgtgacgaat
     7861 ttgaattggc actcttgaag gaagcgcttc gtcagaataa accaattatg gcaatctgtc
     7921 gcggtgtcca acttgtcaat gttgcctttg gtggaaccct caatcaagaa atcgaaggtc
     7981 actggcaagg actacctttc gggacatctc actctattga aacagtagaa ggaagcgtgg
     8041 tggctaagct atttgggaaa gaaagtcagg tcaactcagt ccatcgtcaa agcattaaag
     8101 atttggcacc taatttccgt gtaactgcta ttgattcaag agaccaaact attgaagcga
     8161 ttgagtctat cgacgagcac cgcattatcg gtttacagtg gcatccagag tttctggtta
     8221 atgaagaaga tggcaattta gaattatttg agtatttatt gaatgaactg taacgattgg
     8281 aacatctggt cgttctttct tttatttttt caaaattttt agaatgtggc attttacgca
     8341 aacgtttgaa ttctgataaa aattggagaa attcgacaaa aaaacttgaa aaaaacgaag
     8401 gtaagcgtta tgatagaaaa gaagaaatat tggaggaaga acatgtcaca tattaaattt
     8461 gattattcaa aagttttaga caaatttgtt gcaccacatg aagtggaata catgcaatca
     8521 caagtaacag cagcagatga attgatccgt aaaggaactg gtgctggtag cgacttcttg
     8581 ggatggttgg accttcctga aaaatacgac cgcgaagaat ttgaccgcat cttgaaagct
     8641 gctgagcaaa tcaagtcaga cagcgatgtt ttggttgtga tcggtattgg tggatcttac
     8701 cttggtgcaa aagcagcaat cgacttcttg aatcaccatt ttgctaactt gcaaacaaaa
     8761 gaagaacgca aagctccaca aatcctttac gcaggaaact caatctcatc tacttacctt
     8821 gctgacttgg tagagtatgt tgcagacaaa gacttctcag taaacgtaat ttctaaatca
     8881 ggtacaacaa ctgaaccagc gattgctttc cgtgtcttta aagaactctt ggttaagaaa
     8941 tacggtcaag aagaagctaa caaacgtatc tatgcaacaa ctgaccgcca aaagggtgct
     9001 gttaaggttg aagcagacgc taacggttgg ggaacatttg ttgttccaga tgatatcggt
     9061 ggacgcttct cagtattgac agccgttggt ttgcttccaa tcgcagcatc aggagctgac
     9121 atcaaagctc ttatggaagg tgcgaatgca gctcgcaaag actacacttc agacaaaatc
     9181 tctgaaaacg aagcttacca atacgcagtc gttcgtaaca tcctttatcg taaaggctat
     9241 gcaactgaga tcttggtaaa ctatgagcca tcacttcaat acttctcaga atggtggaaa
     9301 caattggctg gtgaatcaga aggaaaagac caaaaaggta tctacccaac ttcagccaac
     9361 ttctcaactg acttgcactc acttggtcaa tttatccaag aaggaactcg tatcatgttt
     9421 gaaacagttg tccgtgttga caaacctcgt aaaaacgtgc ttattcctac tttggaagaa
     9481 gaccttgacg gacttggtta ccttcaagga aaagacgttg actttgtaaa caaaaaagca
     9541 actgacggtg ttcttcttgc ccacacagat ggtgatgtac caaacatgta tgtgactctt
     9601 ccagagcaag acgctttcac tcttggttac actatctact tcttcgaatt ggcaattgcc
     9661 ctttcaggtt acttgaatgc tatcaaccca tttgaccaac caggtgttga agcttataaa
     9721 cgtaacatgt ttgcccttct tggaaaacca ggatttgaag aattgagcaa agaacttaac
     9781 gcacgtctat aatagaagaa aagagtggtt tgcccactct ttttactctc tttatccata
     9841 gaaattggac tcagccaaga cttgtgatat aatatagaaa gcaaaaaggc agacgcctag
     9901 ataataggag aaactatgtc aaaagatatc cgcgtacgtt acgcaccaag tccaacagga
     9961 ctactacaca tcggaaatgc ccgtacagca ttgtttaact acttgtatgc gcgccatcat
    10021 ggtggaacat ttctcatccg tatcgaagat actgaccgta aacgccatgt cgaggatggt
    10081 gaacgttcac aacttgaaaa ccttcgctgg ttaggcatgg attgggatga aagtccagaa
    10141 tcacatgaga attatcgcca gtctgagcgt ttggacttgt atcaaaaata cattgaccaa
    10201 ctattagctg aaggaaaagc ctataaatct tacgttacag aagaagagtt ggcagctgaa
    10261 cgtgaacgtc aagaagtagc tggcgaaaca ccacgctaca tcaatgaata ccttggtatg
    10321 agtgaagaag aaaaagcggc ttatatcgca gaacgtgaag cagctggtat cataccaaca
    10381 gttcgtttgg ctgtcaatga gtcaggtatc tacaagtggc atgatatggt caaaggtgat
    10441 atcgaatttg aaggtggcaa tatcggcggt gactgggtta tccaaaagaa agacggttac
    10501 ccaacttaca actttgccgt tgttatcgat gaccacgata tgcaaatctc tcatgttatc
    10561 cgtggagatg accatattgc taatacacca aaacagctta tggtctatga agctcttggt
    10621 tgggaagctc cagagtttgg tcacatgacc ttgattatca actctgagac tggtaagaag
    10681 ttatctaaac gtgatactaa tacacttcaa tttatcgaag attaccgaaa gaaaggttac
    10741 cttccagaag cagtctttaa ctttattgct cttcttggtt ggaacccagg tggtgaagat
    10801 gagattttct ctcgtgaaga actcattaaa ctttttgatg aaaaccgcct cagcaagtca
    10861 ccagcagcct ttgatcagaa aaaattagac tggatgagca atgattatat caagaatgca
    10921 gaccttgaaa ccatctttga aatggcaaaa ccattcttag aggaagcagg ccgtttgact
    10981 gacaaggctg aaaaattagt tgagctctat aaaccacaaa tgaaatcagt agatgagatt
    11041 atcccattga cagatctttt cttctcagat ttcccagaat tgacagaagc agagcgcgaa
    11101 gtcatgacgg gtgaaacagt tccaacagtt cttgaagcat tcaaagcaaa acttgaagcg
    11161 atgacagatg atgaatttgt gacagaaaat atcttcccac aaattaaagc agtccaaaaa
    11221 gaaacaggta ttaaagggaa aaatcttttc atgcctattc gtatcgcagt ttcaggcgaa
    11281 atgcatgggc cagaattacc agatacaatt ttcttgcttg gacgtgaaaa atcaattcag
    11341 catatcgaaa acattctaaa agaaatctct aaataaagag gatacaataa tggacatctg
    11401 ggaaaagatg tacgaagaag cacagaaact atacaatcca catgaagtat cagactttgt
    11461 ttatgccaat catgttgtag ctgcagtaga agcaggagat ggacaaatat ttacaggatt
    11521 ctgtatggag ggaacctgtg gtgttttcca tctctgcgca gaacgggcga ccctcttcaa
    11581 tatgtaccaa ttttcaggac aaactaaggt taagaaagta ttagcctttc gagacaaacc
    11641 accttatggt ggaagttcag ccatgccttg cggtgcttgt agagaattcc ttttagagct
    11701 taatgctgaa aataaagatg cagaattcat gatggactat aatataagaa aaacagttaa
    11761 agtcgcagaa ctaatccctt attggtgggg agaagaacgt gcttctaagt ttaatgagtg
    11821 atagaagagt cagtataatt ctggctcttt ttactataag ttgaaaaaat aaaaaaaatt
    11881 agaaaaagta gttgacaaaa tttgaaaaga ctgtataata gtaagagttg aaaataacaa
    11941 ctcaggtccg ttggtcaagg ggttaagaca ccgccttttc acggcggtaa cacgggttcg
    12001 aatcccgtac ggactatggt atgttgcgtc aggagcactt gatgaaaaaa agtttaaaaa
    12061 a
//
LOCUS       DAVLVF010000049        11866 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVLVF010000049 DAVLVF010000000
VERSION     DAVLVF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11866
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.049"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            114..767
                     /locus_tag="VYN66_001348"
     CDS             114..767
                     /locus_tag="VYN66_001348"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV1495361.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            777..1241
                     /locus_tag="VYN66_001349"
     CDS             777..1241
                     /locus_tag="VYN66_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1495362.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1254..2522
                     /locus_tag="VYN66_001350"
     CDS             1254..2522
                     /locus_tag="VYN66_001350"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV1495363.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAAGFSDDGIPLESSKIV
                     KEAMEEAKKFNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2536..2796
                     /locus_tag="VYN66_001351"
     CDS             2536..2796
                     /locus_tag="VYN66_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495364.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2807..4168
                     /gene="pdrM"
                     /locus_tag="VYN66_001352"
     CDS             2807..4168
                     /gene="pdrM"
                     /locus_tag="VYN66_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV1495365.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKC"
     gene            4322..5290
                     /locus_tag="VYN66_001353"
     CDS             4322..5290
                     /locus_tag="VYN66_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV1495366.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5306..6298
                     /locus_tag="VYN66_001354"
     CDS             5306..6298
                     /locus_tag="VYN66_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV1495367.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNSVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6516..7559
                     /locus_tag="VYN66_001355"
     CDS             6516..7559
                     /locus_tag="VYN66_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV1495368.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGKIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7623..9308
                     /gene="lpdA"
                     /locus_tag="VYN66_001356"
     CDS             7623..9308
                     /gene="lpdA"
                     /locus_tag="VYN66_001356"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV1495369.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASSVPVASTSNDDDKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9372..10361
                     /locus_tag="VYN66_001357"
     CDS             9372..10361
                     /locus_tag="VYN66_001357"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV1495370.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGIKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10434..11504)
                     /gene="xerS"
                     /locus_tag="VYN66_001358"
     CDS             complement(10434..11504)
                     /gene="xerS"
                     /locus_tag="VYN66_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV1495371.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         3522 a         2214 c         2662 g         3468 t
ORIGIN      
        1 gtattcaaat cgtatatata gtcgatttat taaatcttta gataaactgt aaaaagtggt
       61 ttccatagcc actttttgct ataatagagg cagtaaaaat gaaggagtcg gctatggaac
      121 actcgtcttg gcatgctttg attaaggcgc aattacctga gggttatttc gggaaaatca
      181 atcagtttat ggagcaggtc tattctcagg ggattattta tccacccaag gaaaaggttt
      241 ttcaggctct cttgacaaca ctgcttgaag aagttaaggt ggtaattcta gggcaagacc
      301 cctatcacgg accaggtcaa gcgcagggct tgagtttttc tgtacctgac tctatcccag
      361 ctccaccatc cttgcaaaat atcttgaaag aattgtcaga tgatatcgga gttaagaaat
      421 ctcatgattt gacagcttgg gctgagcaag gagtcttgct tcttaatgct tgtttgactg
      481 ttcctgctgg acaggccaat ggtcatgctg gtcaaatctg ggagcctttt actgatgctg
      541 tgattcaggt ggtcaatcat ctagatagac cagtcgtttt tgtactctgg ggagcttatg
      601 cacgtaagaa gaaggcatta gttaccaatc ctcatcactt gattatcgaa tcagcccatc
      661 caagtccttt gtcggtttat agaggatttt ggggttccaa gcccttttcc aaggccaatg
      721 cattcttaaa agagacagga caagagccaa tcgattggct tagataagga gaaaatatgc
      781 ctcagttagc gacgatttgc tacattgata atgggaaaga actgctcatg ctccatcgta
      841 ataagaaacc caatgatgtc catgaaggga aatggattgg tgtgggtggt aagctagaga
      901 gaggagagac gccccaggaa tgcgcggcgc gtgaaatcct tgaagaaaca gggctcaaag
      961 ccaagccagt tctaaaaggt gtcatcactt ttcctgaatt tacaccagat ttagactggt
     1021 acacctatgt ttttaaggtg acggagtttg agggcgactt gattgactgc aatgagggga
     1081 cgctagaatg ggttccctat gatgaggttt tgagcaagcc gacttgggaa ggtgaccaca
     1141 cctttgttga gtggctttta gaggataaac ccttcttttc agccaagttt gtttatgatg
     1201 gggataaatt gttggatacc caagttgatt tctatgaata aaggagaaag cagatgctac
     1261 taatcaaaaa tggtcgtgta atggatccca agtctggttt ggatcaagtt tgtgatgtct
     1321 tagttcaaga tgggaaaatt atcaaaattg cgcctgagat cacggaagaa ggagcagaaa
     1381 cgattgatgc tactggtctt gtggttgctc ctggtttggt cgatattcat gtgcatttcc
     1441 gtgaacctgg tcaaacacat aaagaagaca ttcatactgg tgccctggca gccgctgcag
     1501 gtggttttac tactgtcgtc atgatggcta ataccagtcc aaccatttca gacgtggaga
     1561 ctttgcaagc agttctccag tcagctgcca aagagaagat taatgtcaag acagttgcga
     1621 ccattactaa aaactttaat ggtaaaaact tgactgactt taaggcgctc ttagaagctg
     1681 gtgcggctgg tttctctgat gacggtattc cgcttgagag cagtaagatt gtcaaggaag
     1741 ccatggagga agccaaaaaa ttcaatacct ttatcagcct tcatgaggaa gatccaggtt
     1801 tgaacggtgt tcttggcttt aatgaaaata ttgctagaga acatttccat atctgcggtg
     1861 ctactggagt ggctgagtac gctatgatgg cgcgtgatgt catgattgcc tatgcaacta
     1921 aagcccatgt tcacatccag catttgtcta aggaagaaag tgttaaagta gtggagtttg
     1981 ctcaggggtt aggtgcagaa gtcacagcag aagtagcgcc acagcatttc tctaagacag
     2041 aagcacttct tttaacacaa ggtagcaatg ctaagatgaa tccaccgctt cgtttggaat
     2101 cagaccgtcg tgccgttatc gaaggtctca aatcaggtgt catcacagtt attgcgactg
     2161 accacgcgcc tcatcatgta gatgaaaaaa atgttgagga tattaccaaa gcgccatctg
     2221 gtatgactgg cttagaaaca tccctgtctc tcagcttgac ctatttagta gaagctggtg
     2281 agttgagctt gatggaatta cttgaaaaaa tgacatacaa cccagccaag ctttacaact
     2341 ttgaagcagg ttacttggct gagaatggtc cagcagatat cactattttt gatgccaagg
     2401 ctgaccgctt tgtggactcc cattttgctt ccaaagcagc taattcacca ttcatcggtg
     2461 aaaccttaaa agggcaggtt aaatatacca tctgtaaggg acaaatcgtc tatcaagctt
     2521 gataggagtt agaatatgaa ttattttcga aaacgtaggg agagacaagc gaaatctaat
     2581 agtggaattt actgtcccgc agctaatcgt tccaaaattc aatatgaaac taaagaaaaa
     2641 gctgaccatg cggttcaata tgataacgga ggtttagtta gaagctatta ttgtcgtaca
     2701 tgtgcgtgtt ggcatactac ttcaaaatct aataaacctc cgctaagttt gaaaaactat
     2761 ggtctgaaac tctttggttt agataaatca gaatagaaag agaagtatgt ataagacaaa
     2821 gtgtttacga gagaagttag tattattttt aaaaattttc ttcccaatcc tgatctacca
     2881 atttgccaat tattctgcct cttttgttga tactgcaatg acaggtcaat acaacactat
     2941 ggacttggct ggtgtatcta tggcaaccag tatctggaat cctttcttta catttctaac
     3001 agggattgtg tcagccttgg tgcctatcat tggtcaccat cttggtcgag gcaaaaagga
     3061 agaagttgcg tctgattttt accaatttat ttatttggcc ttgggcctat ctgtggtctt
     3121 gctggggatg gtacttttct tggcaccaat aatcttgaat catattgggt tagaagcagc
     3181 agtagcggca gtagcggttc gctatctttg gtttttatct atcgggatta tccccttgtt
     3241 gctctttagc gtcattcgtt ccttgctgga ttcgctgggc ttgaccaaac tgtccatgta
     3301 cctcatgctt ttgttactcc ctctcaatag cggatttaac tatctcttga tttacggtgc
     3361 ctttggtgtt ccagaactgg gaggggctgg tgctggttta ggaacatcct tggcctactg
     3421 ggtcttgctt gggatttctg ttctggtttt atttaaacag gagaagctca aagccttaca
     3481 ccttgagaaa cgaattccac ttaatatgga taacattaag gaaggagttc gtttaggtct
     3541 gcctattggg ggaactgtct tcgcggaagt ggctatcttt tcagtggttg gcttgattat
     3601 ggctaagttt tcgtccttga ttatagctag tcaccagtca gctatgaact tttcaagtct
     3661 tatgtacgcc tttcctatga gtatctcatc ggctatggct attgtcgttt cctatgaagt
     3721 gggagccaag cgatttgatg atgcgaaaac ctatattggt ctaggaagat ggactgctct
     3781 catttttgcg gccttcacct taaccttcct ttacattttt aggggaaatg tggccagtct
     3841 ttatggtaac gacccaaaat ttatcgattt gacagtgcgt tttttaactt atagtctttt
     3901 cttccagtta gcagatacct ttgcggcgcc gcttcaggga attttgcggg ggtataagga
     3961 tacagttatt cctttttacc ttggtttgct tggttattgg ggcgtagcaa tccctgtggc
     4021 tacgctattt gattccctaa cagattttgg agcttattct tactggatcg gcttgattat
     4081 tagtttgatt gtgagcgggg cgctctaccg ttggcgttta actgtgatta tgaagagatt
     4141 tgaatcttta gcaaaatcca aatgctaaaa agtttgtgaa aaaatattct tgacaagaaa
     4201 atcccttgtt ttcacggggt tttctttttt attttgtgaa atttctttta gaaaaacttt
     4261 gacagtattt gccaaaagcg gtaaaatagt aaggtaagaa gaaagttaga aaggcaagaa
     4321 gatgtcaact ttagataaaa atcttttgct agaaatgttc cgtaagatgg aagaaatccg
     4381 tcgcatggac ttaaaaattg cacaattagt aaagaaaggg aaagtgccag gaatgacgca
     4441 cttttctgtt ggtgaagagg cagctaacgt gggggctatg ttagctctca atccagatga
     4501 tctgattacc tcaaaccacc gtggacacgg gcaagctatt gctaaaggga ttgacctcaa
     4561 cggaatgatg gctgaaatcc ttggtaaata cactggaacc tgtaaaggga aaggtggatc
     4621 tatgcatatc gctgaccttg atgctgggaa ccttggtgcc aatggtatcg taggtggtgg
     4681 tatggggatc gctgtcggtg cagccctcag tcagcaaatg caaaataccg gtaaaatcgt
     4741 tgtctgcttc tttggagatg gtgcgaccaa tgaaggtgtt ttccacgaag cagtgaacat
     4801 ggcttctatc tggaacctgc cagtcatttt ctattgcatt aacaacggtt acggtatctc
     4861 tgcggatatc aagaaaatga ccaatataga acatatccat caacgtagcg ccgcttatgg
     4921 aattcctgga atgttcatcg aagacggtaa caatgtcatc gatgtctatg aaggatttca
     4981 gaaagctgtg gaccatgttc gcagtggcaa tggtccagtc ttgattgaaa gtgtaactta
     5041 tcgctggctc ggtcactcat catctgaccc tggtaaatat cgtacgcgtg aagaagtgga
     5101 attgtggaaa caaaaagatc caatcgaaaa cctccgcaat taccttattg aaaataacat
     5161 tgcaagtgcc gaagaattgg aagaaatcca agcgcaagta aaggaagcag tagaagcttc
     5221 tgttaaattt gcagaggaaa gcccattccc accgcttgaa tcagcctttg aagatattta
     5281 cgcagactaa ggagaaagag ataaaatgga aacaaaaaca atgtccttcc gtgacaccat
     5341 tatccttgct atgtctgagg aaatgcgtcg cgatgaaaat gtgttcttga tgggagaaga
     5401 cgtcggtgtc ttcggaggag acttcggtac ttctgttgga atgcttgaag aatttggtcc
     5461 agaacgtgtc cgtgactgtc cgatttctga agctgccatc tctggagcag cagcaggagc
     5521 agccatgaca ggacttcgtc caatcgtcga tatgaccttc atggacttct ctgttattgc
     5581 catggacaat atcgtcaacc aagctgctaa aacacgttac atgtttggtg gtaaaggtca
     5641 ggttccaatg actgttcgtt gtgcagctgg taacggagtt ggctctgccg cccagcactc
     5701 acaatctcta gagtcttggt ttactcacat tccaggactt aaggttgtgg cacctggaac
     5761 acctgcggac atgaaaggcc tgcttaagtc ttctatccgt gataacaact cagttattat
     5821 acttgagtac aagtcagaat ttaaccaaaa aggggaagtg ccagttgatc cagactacac
     5881 aattccactt ggggttggtg aaatcaaacg ccaaggaacg gatgtaacag ttgttactta
     5941 tggaaaaatg cttcgccgtg tggttcaagc tgctgaagaa ttagcagaag aaggaatttc
     6001 agttgaaatt gttgacccac gtacccttgt tccgcttgat aaggatatca tcataaactc
     6061 agtgaagaaa actggtaagg ttgttctggt caacgatgcc cacaaaacaa gcggctatat
     6121 cggtgaaatt tcagctatta tttcagaatc agaagcattt gactatctag atgcaccaat
     6181 ccgccgttgt gcaggagaag atgtgccgat gccttatgca caaaacctag aaaatgcaat
     6241 gattccaaca gttgaaagta tcaaagatgc aatccgaaaa acttataaca aagaataggg
     6301 ttacataaga taagttatgt aaataaaata aagctgacga gaaactttgt atcttttagg
     6361 tgcagagctc ttcgtcatta atatcttaga ttagagcacg ggctaaaaga tcggaaaaaa
     6421 gataaatctc cttggcttca tcgaagccat cgtcgatttc ctatttttca gtcgcttttg
     6481 acgccctttg tatcataaat agaattggag aggtcatggc tgatgacaag ctaagagcga
     6541 ctcctgcagc tagaaagtta gcggatgatt tagggataaa cctctacgac gtttctggct
     6601 caggtgcaaa cggtcgtgtc cacaaagaag acgtggaaac ttataaagac acaaacgtgg
     6661 ttcgcatttc gccacttgca aaacgaattg ccctcgaaca taacattgct tggcaggaaa
     6721 tccaaggaac cggtcatcgt ggtaaaatca tgaagaagga tgttttggcc ctgcttcctg
     6781 aaaatatcga aaacgatagc atcaaatctc ctgctcagat tgaaaaagtg gaagaagtcc
     6841 ctgataacgt aacaccatat ggtaaaatcg agcgtattcc aatgacacca atgcgtaagg
     6901 ttattgccca acgcatggtt gaatcttact tgactgcgcc aaccttcacc ctcaactatg
     6961 aagttgatat gactgaaatg ttggctcttc gtaagaaggt tcttgagcca atcatggaag
     7021 caactgggaa gaagactact gtaacagacc ttctttcgct tgcagttgtt aagactctta
     7081 tgaaacaccc atacatcaac gcttcattga cagaagatgg caagactatt atcactcaca
     7141 actatgtcaa tcttgccatg gcagttggga tggataatgg attgatgaca cctgttgttt
     7201 acaatgctga gaagatgagt ctttcagaac tggttgtagc ctttaaggat gttattggcc
     7261 gtaccttgga tggtaaattg gctccaagtg agttacaaaa ttcaacattc acaatcagta
     7321 atttgggaat gtttggtgtt cagtcctttg gtccgattat taaccaaccc aactcagcta
     7381 tccttggtgt cagttcgaca atcgagaagc cagttgtcgt caatggtgaa attgtgattc
     7441 gcccaatcat gagtttagga ttaaccattg accaccgtgt cgtagatggt atggctggtg
     7501 ctaagtttat gaaggacttg aaagagttga ttgaaacgcc aatctcaatg ttaatttaag
     7561 agcaagagta ttcattttta agatatagat aaaggaagga agacatggcc ttagaagtaa
     7621 ttatgccaaa agccggcgtg gatatgacag aaggacaaat cgtccaatgg aataaaaaag
     7681 tcggagaatt tgtaaaagaa ggagaaatcc ttttggaaat catgactgat aaagtcagca
     7741 tggaattgga agccgaagaa gatgggtact tgattgccat tctcaaagga gatggtgaaa
     7801 ctgtccctgt aacggaagtt atcggttacc ttggtgaaga aagggaaaac atcccaacag
     7861 ctggagcagc atcgccagaa gctagctctg tacctgtagc aagtacctca aacgatgatg
     7921 ataagagcga tgatgctttt gatatcgttg tgattggtgg aggtcctgct ggttatgttg
     7981 cagccattaa agctgcccaa ttcggcggta aggttgccct tgttgagaaa tctgaacttg
     8041 gtggaacctg cttaaaccgt ggatgtattc caaccaagac ctaccttcat aacgctgaaa
     8101 ttattgaaaa tatcggtcat gctgcaaatc gtggtatcgt gattgaaaat cctaacttca
     8161 ctgttgatat ggaaaaactt ttagaaacta aatctaaagt tgttaatact cttgttggtg
     8221 gtgttgcagg acttcttcgt agctacggag ttactgttca taaaggaatt ggtacaatca
     8281 ctaaagacaa gaacgtcttg gtaaatggtt ctgaattgct tgaaaccaag aaaatcattc
     8341 ttgctggtgg ttcaaaagtc agcaagatca acgtccctgg tatggaatct ccacttgtca
     8401 tgactagtga tgacattctt gaaatgaacg aagtgccaga aagccttgtt atcatcggtg
     8461 gtggagttgt cggtatcgaa ctcggtcagg ccttcatgac atttggttca aaagtgactg
     8521 ttatcgaaat gatggaccgt atagttccag ctatggatgt ggaagtttct aagaaccttc
     8581 gcttgattct tgagcgtaaa ggaatgacca tcttgactgg tactaaactg caagaaatca
     8641 ttgaggaaaa tggtcaactt cgtatcaagg ttgaaggaaa agacgatatc atcgcaagca
     8701 aagctcttct ttcaattggt cgtatgccag accttgaagg tattggagag gttgagtttg
     8761 aattggatcg tggttgtatc aaggtcaacg aatacatgga aacttcagtt ccaggcattt
     8821 atgcaccagg tgacatcaac ggtactaaga tgttggctca cgcagctttc cgcatgggtg
     8881 aagtttccgc tgaaaatgcc cttaaaggaa atcatgcagt tgccaaattg aatttgactc
     8941 ctgcagccat ctacactctc cctgaagtag cagcagtagg tttgacagaa gaacaagccc
     9001 gtgagaaata cgatgttgcc atcggtaagt ttaactttgc tgctaacggt cgtgctattg
     9061 catctgacgc agctcaaggt ttcgtaaaag ttatcgctga taagaaatac ggagaaatcc
     9121 ttggtgtaca cattattggt cctgcagccg cagaattaat aaacgaagca tcaagcatca
     9181 tcgaaatgga aatcactgtt gaggaaatgc tgaagaccat ccacgggcac ccaacttact
     9241 ctgaagtgat gtacgaagcg tttgcagatg ttctaggaat ggccatccat tcacctaaga
     9301 aaaaataaaa cgaagataga ttagactgga aggttatttc cagtttctat catataaagt
     9361 aaagggatat catgaaatac attatcaatc attcaaatga cactgctttt aatattgcct
     9421 tggaagaata cgcctttaaa caccttttgg atgaggatca aatcttccta ctttggatta
     9481 acaagccatc tatcattgtt ggtcgtcacc agaacactat cgaagaaatc aaccgtgatt
     9541 atgttcgcga aaatggtatt gaggtagttc gccgtattag tggtggtgga gctgtttacc
     9601 acgatttaaa taacctcaac tacacgatca tctccaaaga agatgaaaat aaggcatttg
     9661 acttcaagag cttctcaact ccggttatca ataccctggc tcaacttggg gttaaagctg
     9721 agttcacagg tcgtaatgac cttgagattg atggtaagaa gttctgtggc aatgcccaag
     9781 cctatatcaa cggccgtatc atgcaccacg gttgcttgct ctttgacgtt gatttgtcag
     9841 tcctcgcaaa tgcccttaag gtttcaaaag ataaatttga atcaaaaggt gtgaaatccg
     9901 tccgtgcccg tgtaaccaat attatcaatg aattaccaaa aaaaatcaca gtcgaaaaat
     9961 tccgtgattt actcttggaa tacatgaaaa aagagtaccc agagatgact gaatacgtct
    10021 tttcagaaga agaattggcc gaaatcaatc gcatcaagga tactaagttt ggaacttggg
    10081 actggaacta tggtaaatca cctgaattta acgtccgtcg tggaatcaaa ttcactagtg
    10141 gtaaggttga agtctttgct aacgttactg aatcaaaaat ccaagacatc aagatttatg
    10201 gtgacttctt tggtattgaa gacgttgctg cagtagaaga tgtccttcgt ggggtgaaat
    10261 acgaacgcga agatgttctt aaggcgctaa aaaccattga tatcacacgc tacttcgctg
    10321 gtattagccg tgaagaaatc gctgaagcgg tagttggata aatcaaaaag aagttggcca
    10381 actaaccaac ttcttttttt gatatttatt tacataattt aaaatacgta aaatcataaa
    10441 ctatccagag cattcttttg ttcatcatta acaatatggg tatagaggtc agtgacttgt
    10501 gtgctggcat gtcctagttg gtgactgact aaaacttgtg atttagtagc atcatagagc
    10561 ctagttgcta gtgtatggcg cagtttatgg ggtgttacac gtactttaaa atcctctgag
    10621 tatttagcaa ccattttctc aacgctagaa gcatcgatac gattaggaac acctctgtag
    10681 agagttaaaa aaagggctgt atctgttttt tccgttttat agcgttgatt ccgaatggcc
    10741 agataattct ctaaataagg tttagcaaaa gcagcgacat tgactgagtc acgtttgcca
    10801 ccttttcgag taacatcaat aaccatcatt tttagattga gatctcttag atctagatta
    10861 acagcttcag ataagcgaac accagatgcc aagagaaggg caataatggc taaatctcgt
    10921 tctttatttt tgttgaatga tgagagagct cgatttgaaa gctgttgtgg gtgctcttga
    10981 tcgatataag ttagaaaacc ttctgtttca tcacctagaa agagtttttg cttgatattt
    11041 tcagctctgg cagcaagggt ttctttcttt ttcttggttg aaactttttt cattacatta
    11101 cgatagaaat aaggttcccc ctgatcgttt tcaacctcct cggttagata cttgtaaaga
    11161 ctagaaagtg ctgataaggt tcgattgata gttgtctgtg aaacaccttg ttttgttgta
    11221 ttagcattca gcaagggacg ttcacgtaga taaaggataa aggattccat gtctttctta
    11281 gacatatttt ccaaaactga taaaggaata tcggatattt tatcagcgtt tgaaatacca
    11341 gactccaaaa cccagctgaa aaatcggtca tattccttaa ggtattcgta cagggttgta
    11401 aaactgtagg gcacagccag tttagattgg tagtattcca gaacatacca gggcatgagt
    11461 tgttttagtt tgtcgattcg ttccagtaaa atctcacgtt tcatgtattt tctccataat
    11521 taagtatact agtagtatag catagacttg tatatttttc aagaaaatta tagtttttca
    11581 gaaagatgct aaaagatgct ataggagttt ttcaaagtga taaaaagact tagacctaca
    11641 aacaaaacag aataaggaaa ataataaaaa tctcacccac taaacttctg ttaatactac
    11701 ataactgaag agctatataa ggagctatga gatacaaaac acattgtata atagattgaa
    11761 actagaatag tacatctgga cttctaaaac attgctagaa attgatttga ttgtcctgat
    11821 tgttttatcc tatttctatt tcatttctct atataattgc tattgt
//
LOCUS       DAVLVF010000050        11806 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVLVF010000050 DAVLVF010000000
VERSION     DAVLVF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.050"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(151..672)
                     /locus_tag="VYN66_001359"
     CDS             complement(151..672)
                     /locus_tag="VYN66_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1495372.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPDPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAS
                     LGLGFFKKKEDEK"
     gene            complement(630..1736)
                     /locus_tag="VYN66_001360"
     CDS             complement(630..1736)
                     /locus_tag="VYN66_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV1495373.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVVVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(1847..2101)
                     /locus_tag="VYN66_001361"
     CDS             complement(1847..2101)
                     /locus_tag="VYN66_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495374.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(2441..2974)
                     /locus_tag="VYN66_001362"
     CDS             complement(2441..2974)
                     /locus_tag="VYN66_001362"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1495375.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     RAPRGEKVYKKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            complement(2961..3176)
                     /locus_tag="VYN66_001363"
     CDS             complement(2961..3176)
                     /locus_tag="VYN66_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1495376.1"
                     /translation="MGKERCEQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCALPSVWAALKYIKVTLKKDDEL"
     gene            complement(3143..3286)
                     /locus_tag="VYN66_001364"
     CDS             complement(3143..3286)
                     /locus_tag="VYN66_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEV1495377.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            3520..5238
                     /locus_tag="VYN66_001365"
     CDS             3520..5238
                     /locus_tag="VYN66_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV1495378.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            5349..5696
                     /locus_tag="VYN66_001366"
     CDS             5349..5696
                     /locus_tag="VYN66_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1495379.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(5975..6811)
                     /locus_tag="VYN66_001367"
     CDS             complement(5975..6811)
                     /locus_tag="VYN66_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1495380.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(6824..7453)
                     /locus_tag="VYN66_001368"
     CDS             complement(6824..7453)
                     /locus_tag="VYN66_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1495381.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(7463..8104)
                     /locus_tag="VYN66_001369"
     CDS             complement(7463..8104)
                     /locus_tag="VYN66_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1495382.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(8250..9479)
                     /locus_tag="VYN66_001370"
     CDS             complement(8250..9479)
                     /locus_tag="VYN66_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1495383.1"
                     /translation="MNNSQQMLQALEEQDLTKAEYYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLMLGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLAAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(9469..10635)
                     /locus_tag="VYN66_001371"
     CDS             complement(9469..10635)
                     /locus_tag="VYN66_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1495384.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVIFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            10779..11792
                     /locus_tag="VYN66_001372"
     CDS             10779..11792
                     /locus_tag="VYN66_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV1495385.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNEFHVPEAVCIR
                     FAP"
BASE COUNT         3552 a         2557 c         2065 g         3632 t
ORIGIN      
        1 ctggaaatat ttttaaaaaa ataggattca tagtttccca gttcaacgga cttgtaagcg
       61 tttttattct tcacgaaaga cctcgttctc aaaaccagga aaaaagccca catctgtgag
      121 cttttcctgg ttcctagatt ctaaaatcta ttatttttca tcttcttttt tcttgaagaa
      181 tcctaatcct agactagcta acaaggtcat gataccagca ctagccaagg tagcattagc
      241 ttctgtacct gtgtttggca attcctctct cttacctgtc tcataagtcg gaacttctgg
      301 gtctggattc actggagttt cagtttttgg agtacctggg tctggagttg gtttatctgg
      361 tgttgataaa cggtcatacc ttaccgttat ttctttatca ctagagtctg acgtaacttc
      421 ttgtgattca actgttggaa tatctggatc tttgtacttg tcaatcttac cagatacaac
      481 ctcgtcccag tttcctgttg tccattcacc gtaggttaca actcccgtga ccttgttctc
      541 agtttttgta cggcttaagg ttacaggttg aacaacatct tcttttacat tttggttcgt
      601 aactttatca acgtaatgaa tgatacgcgt tatagtcttc gtctcagtag aggttgctgt
      661 tttgggaacc actgtttcct caacattctc acggtagtaa tagtcaactg ttgcaccgtc
      721 ttctggtacg catttgcagg agttgctacc aaggtgtatg ttgttttcct tgtgataact
      781 cggtcttctt tgtcctcagt tgttgttttc ccttcaatag tttttgattc tgtggtatac
      841 tcagaaccta tcgctaaatc agcttttata acagactctg ccaacttctc ttggctacct
      901 tctttatagt aattggctgt tactgtagca gtggttggcg cttcgctttc tctataaact
      961 aaggtcactg ttctaccttc gcttacaata ttcccagtta aacttgcaga atttgtatct
     1021 gcttctttaa aagtataata ttttccgtca gtagtagtca tgctactgag ttttttatct
     1081 gtgacattgt agctggtacc aatcagttgt tttttattgg taatgtaggt tccgtcactt
     1141 tctttttctc caattccagt atcatttttc aatgatagca aacgcccttg ttcatcaaca
     1201 tagcgaactt tcacattttc tgagattagt tctgccaatt ctgaggtttt tttctttttc
     1261 ttgatttctt cggttatttt ccctttctct tcttcgggaa tatttagttt tggaatgatt
     1321 ttttcaacaa cggttcgtga tggttccaca gtatcttgga tgactgaaaa gtcagctaga
     1381 attgggagat tatactgaac acggtgactt tgagtgttta ctactactct ttcattattc
     1441 tctgaaaata cttgtacggt ataagaaaca acatcttttc ctaatagaac atccccagta
     1501 gagaaatagc cgccttttcc tagtttgcta tctccagagc ccacttcttt cctaatctta
     1561 tcagatagtt ttttaccagt cagtacattc gttcgcacaa tccctttgtc tacccctaca
     1621 aagtgggaga actttttgaa ctcttcagaa ccagatctag cccaaccatt attaagggca
     1681 tttgcttttg tatttgtatt ctctctcaaa agtttggcga ttagaattat attcatcggc
     1741 acttagagtt gctgctatat ctgactcttg aataccaact tccttactac catttctagc
     1801 ggcagtatat gtgtatttat ttgtttctac ttctagctga ttatccttaa catgataggt
     1861 aattttctcc tggtataaga gtcttcagta gtttatatgc tctgtcatca tctttagctt
     1921 tagaagcagc tgagtttagc caagttgggg tattaggagt atctttatca gcctgtttca
     1981 agacattatt caagtcattt gctagcctat tggttgtcct cactgtgaaa gttttcttcc
     2041 agtctgaacc acttttatta gacttagata tgctcatgaa atcctggtca acattcaaca
     2101 caatatcgcc ttgtttttta gcatcttccg ttgcttctgc ttcaaattga tcatggagtt
     2161 tttgttgctc tggtgtatag tttttttatt tacacgattt aatgtactat tttttatttt
     2221 agtattaaga actccattaa tacgattttt aaactctgct tcaaaaaatc ccttaatttg
     2281 ttggtgcgca agttcaacat tcaaaccttt cttatacaag tcttcattct aaacgatttc
     2341 ggctacacgg tgaatggttt gattgtatac cgtcttctct taaccagcag tatccttagt
     2401 tttaatgact actcgatagg ttcctttaga accgttatag tcatctagtt ttaaaaaagc
     2461 attccaaaca ttcaaaaata gttggaactt cccttaataa atccgtcact ttctttttca
     2521 agatagccca agcttgctca ataggattca aatccggtga ataaggtggt agaggtaaga
     2581 aaaagtgttt atcttggatg cagagttcat ccaaaatgtt cttgggatga aaactagcat
     2641 tgcccatgac aataacatga ggtgtcttca aagcaggtag gagttgcgtt ttgaaccact
     2701 ccacaaagaa atcgcttgtc atgcttttct tgtaaatcat gggagctata aactctccgt
     2761 ctacttgtcc tgcaacaatt gaagttcgct caaaacgacg tccgctaatc tttttataga
     2821 ctttctcccc tctaggagcc cgtgcataag gacgatagag gtagcggtcg attcctgttt
     2881 catcaatata tacgattggt aaatctttta ggttatccaa aatatcaaga aactcagcga
     2941 ctttctctgg ctcttgttcc ttaaagctcg tcatcttttt ttaaagtgac cttaatatac
     3001 tttaaagctg cccatacgga gggcagagca caatcaaaat gtgccgcaat ttcccgtaaa
     3061 aaagcatctg gatgagcctc tacaaaggct ttcaattcct ctaaaggaat ctttcggttt
     3121 ttgacgactc gctttttcct ctctatgtgt ccttgttcac atctttcttt tcccacgtga
     3181 agagagttct gacgccaaca ccaaaaaact tggctgcctc gacatggctg tgcccctctt
     3241 tgatgtaatc taatgctcgc tgtttaaaat cagtactata tgccatagct ttattataat
     3301 atgcttgttt aaaactaagc aactatatca atgttttgtt attagaagac ttactttttt
     3361 tactgaaatc gaatttttcc taacctcaac atcatataac cccaaaattc aatctgacct
     3421 ggtttgaaga gatgttcgaa gcagataagg ttttactaaa actaaaaaaa atgatataat
     3481 caaactgatt aaatgtttta aaaataaagg agaatttgaa tgtcttacca agaaaattac
     3541 cagaaatggg ttgattttgt ggagcttcct gactaccttc gtcaagattt ggaaaatatg
     3601 gacgaaaaaa ctaaggaaga tgccttctat acaaatcttg aatttggtac tgcaggtatg
     3661 cgtggcttgg ttggtgctgg tacaaaccgt atcaacatct acgttgttcg acaagctact
     3721 gaaggattgg ctcgtttgat tgagtcaaaa ggtggaaacg agaaagaacg cggtgtagca
     3781 attgcctacg atagccgtca cttctcacct gagtttgcct ttgaatctgc ggcagttctt
     3841 gctaaacacg gcatcaaatc ttacgtattt gaaagccttc gtccgactcc agaactatca
     3901 tttgcagttc gtcatctcaa ctgtttcgca ggtatcatgg tcacagccag ccacaaccct
     3961 gctccattta acggttacaa ggtttacggt gaagacggtg gacaaatgcc tccacacgat
     4021 gcggacgctt tgactactta tatccgtgca atcgaaaacc catttgcagt tgaagttgct
     4081 gatgtggaaa ctgaaaaagc ttctggcttg attgaagtta tcggcgaagc tgttgacgta
     4141 gaatacctta aagaggttaa ggacgtaaac atcaacccag ccttgattga agaatttggt
     4201 aaagacatga agattgtcta cacaccactt catggtactg gtgaaatgtt ggctcgtcgt
     4261 gctcttgccc aagcaggatt tgactctgtt caagttgttg aagcgcaagc aactgctgac
     4321 ccagacttct caactgtaac atctccaaac ccagaaagcc aagcagcctt tgcccttgct
     4381 gaagaacttg gtcgtcaagt tggtgcagat gttcttgtcg caactgaccc agacgctgac
     4441 cgtgttggtg ttgaagttct tcaaaaagat ggtagctacc tcaacctttc aggtaaccaa
     4501 atcggtgcta tcatggctaa atacatcttg gaagctcaca aaaacgctgg aactcttcct
     4561 gaaaatgccg ctctctgcaa atctatcgtt tcaactgact tggtaacgaa gattgctgaa
     4621 agctacggcg caactatgtt caacgtcttg acaggtttca aatttatcgc tgaaaaaatc
     4681 caagaattcg aagaaaaaca caaccacact tacatgatgg gatttgaaga aagcttcggt
     4741 tacttgatta aaccatttgt acgtgataaa gatgccatcc aagccgttct tgtcgttgct
     4801 gaacttgctg cctactaccg ttctcgtggt ttgacacttg ctgacggtat cgaagaaatc
     4861 tataaagaat atggctacta cgcagaaaaa acaatctctg ttactctttc aggtgtcgat
     4921 ggtgctgaac aaatcaaagc gattatggct aaattccgca acaatgctcc aacagaatgg
     4981 aacgcaacag ctatcactgt cgtagaagac ttcaaggcac aaactgctac tgttgctgac
     5041 ggtactgtta caaacttgac aactcctcca agtgatgtgt tgaaatacac acttgctgac
     5101 ggttcatgga ttgccgttcg cccttcaggt acagaaccaa aaatcaagtt ctacattgca
     5161 gttgtagggg aaaccaacga agaatcacaa gctaagatta ctaacatcga agcagaaatc
     5221 aatgcatttg taaaataaag acctataaaa gatgagacca accaacctca tcttttttcg
     5281 tatcaaatct aagcaatttt agaaacttta tggcatacta gacttatcaa tgaaagcgag
     5341 gtaatctcat ggaacaattt ttagataaca tcaaagacct tgaagtcact acagttgtgc
     5401 gtgcgcaaga agctcttgat aaaaaagaaa ctgcaacctt ctttatcggt cgcaaaactt
     5461 gcccttactg ccgtaaattt gcaggtacat tgtcaggtgt cgtagctgaa accaaagctc
     5521 acatttactt catcaatagt gaagaaccaa gccaactcaa tgatttgcaa gcattccgct
     5581 cacgttatgg aatcccaact gtaccaggtt ttattcatat tacagatgga caaatcaacg
     5641 tccgttgcga ctcttcaatg tcagcacaag aaatcaaaga tttcgcagga ttgtaaaaaa
     5701 tcagctaaca agtcttaaaa tcacaaaaac gcataatgta aggtgcaaaa accttgatat
     5761 tatgcgtttt attgttccta aaacagttac atcagcattt cattacagaa cattttaatt
     5821 ttggaagtca aatcaatttt taacaatgtt ttagaaatca cagtgggcta ttccatattc
     5881 aattatccag attcaatcgc tattatcgtt ttcctagatt acaaaaaaaa ctacgttttg
     5941 ctaatcaaaa cgtagtctca gccactattt tatcttactg tccttctttt acttctttgg
     6001 ttgctacatc ttctccaaac catttttggc tgatttcttg gaacttgccg tccttgtaaa
     6061 gactagaaaa agcttcattt atcttcttaa ccaaggttgt atcttcctta cgggctccaa
     6121 ccgcaaaagc ttctgtttct agtccaactg taaagacatt ataatcgttt aaaacacctt
     6181 ctgcttctaa ataatagttt gcatagacac ggtcaatcaa tagaccatca attcgatcgt
     6241 ttttcaaatc aatcaaggct tcattaaagg tttggtattg attcgcttcc ttattagcga
     6301 caatattctt caaaatttct ggatttgctt caaagtccgc ataaccagat gaaccagctt
     6361 gagctcctaa tgtctttcca gtcatatcct ttgcagtcgt gataccagat gattttttcg
     6421 taaccaatac ctgctcattc ttcatatatg agttactgaa agccaccttt tcacggcgtt
     6481 cgtctgtagc ggaatagcca ttccaaatca gatcaatcgt tccttttgtc aattcagctt
     6541 ctttcaaatc ccaatcaatc ggttgccaat ttaccgtgat tccgtatttt tcaaaaacag
     6601 ctgtagctaa atcaatatca aatcctgcat aagaaccatc tttctgagca aatcccattg
     6661 gaacaaaagt actatcaaat ccaatagtaa tagacttgtt agactggtac tttgaccaat
     6721 tatctccact agtttcgcta gaatttttcc cacaagctac taagaacaaa gcagtcatca
     6781 gactgactaa tacaagcatc cattttttca tccatttttc ctcctattta ggttctactt
     6841 tcaataatac atctgcgata ttttcagcaa actgcaaatc atgggtaacc acaatctggg
     6901 tcatcccaag ttccctattt tgcaagatta gcttctccac ttccaaacgt aattctggat
     6961 ccagggcaga agttggttca tcgtagccaa tgatttctgg gtcaatcatc atagcacgcg
     7021 ccaaagccac ccgctgcttt tgcccaccag atagtgagaa aggataggcc tctgcgtgtc
     7081 ctcctagtcc taactgttcc aagagtccac tcgccttctt ctcagcctct tcctgcttca
     7141 ttcccatggt cttcacaggc gataaagtca aattttccag aactgataga tgaggaaata
     7201 gttgaaaatc ttggaagaca aatcctagta ggttgcgctt ctgcaattca tccagctcta
     7261 aaggttgtcc attataaaag atttgccctg aatcaatggt ttcaagaccc gcaagcatac
     7321 gtaaaagagt cgtcttacct ccaccagaag gtccaacgat agccaggatt tgcttttcag
     7381 gaatacttag actgaaatta gacaggattt gtttgtctcc aaagacttta ttgatatttc
     7441 gtaattctaa catggcagcc tcctatctat aataactata cttcttctca acttttttgg
     7501 aaataattgt cacaatccca atcaaaatca aataaatggc tcctgccaag aacataggaa
     7561 ctagactagc atcgcggtta gcagctgttc gactagccaa gataaggtct gaaattccga
     7621 gagcatagac caaagaagta tccttgacca aactcataac ttcattaaag acactaggaa
     7681 gaacgatctt ggtcacttgg ggcaagataa tatagcgcac tctgtcaaaa gggctaaact
     7741 tcaagacctt ggcggcctca tactgtcctc ttggaatagt gtcaattccc ccacggaaaa
     7801 tttctgcaaa gtaagctgca tagttgagaa caaaggcaat aatagctgca ggaaggcggt
     7861 ctaaacgaat cccaatactt gggagcacat aatagataaa aattagttgc aagagtaagg
     7921 gggttcctcg cataacccag atgtaaatgt taatcagata atggaggggc ttccaatgga
     7981 cttgcaaggc aaaagcaatc aaaacgccca agggaatcga aaatagcaag accagtgcaa
     8041 agacctgtac agtcgtgctc gcaccattca gtaaactcgg taatatctca aataaataag
     8101 acatactgca cctcctaaaa gtaattgttc ctattatagc atgaataaac aagattacca
     8161 agtatttttg taaaaagatt ctaccatata caaataaaaa ccgtagctat caaaagagat
     8221 aaactacagt tctatatgtt taaacattct tacaatctct caaacagttc ttgcatttgc
     8281 acatcatctg gaaccagttt taagtaagtg tgagcatgga cttttgcttc ttcaaaatgt
     8341 cccaattcac gcaagagata gatatagtgt tccagaaatt ctggattgtc cttcaaatct
     8401 cctgtcaact cttgataatg ctcataagca gtatccaaat cgtccatttc ttgataagaa
     8461 cgagcaatca tccacttggt caaaagattt tctggctcct cactctgcaa gtctagaata
     8521 tcctcataac gctcctgctc cagataaata gtggctaaac gaagcaagat ttcttctgta
     8581 tcctcagcgt cttcttttgc agcaagaaga taattttctg caccactagc atcatgcaat
     8641 tcataagaaa attgtgaagc agctagcaag aggcgagttt caaagggatt tttctctaat
     8701 ccttgcttag cgatacgcag ggcttcttga acttgatgtt ccttatgtaa agcctgactg
     8761 tacccatact catagccttc aaagtcagga gaaatggtat caagctgctt aaagtagagg
     8821 gtggcttttt gatattcttc ttgatcaaaa taaagactgg ccaactcaaa agctgttaag
     8881 tcatcgtatt ctaactccag ggctttttct aaaaactcag tagccgtttc aaatttccct
     8941 aactgagcat aggcaaagcc aattcgttga taggtggaaa tgcccgtttg ctcataaatc
     9001 gagcgattat ctaactgggc ataggcttga atagccgctt ggtaattttc caactcacta
     9061 tccaactctg ccaaacccaa catcaagaga gaatcctctg agtaggtcaa ggcctccaat
     9121 aatttctcac gtgccacatc tgtcaaacct tccagctggt aaaggtctgc cttcaaaacc
     9181 aaagacgaga cataccagtc actgtcagct tggatttcct caagataggt aaaggcttct
     9241 tctatttgac catcctcgct agcaattgca gctagattaa gatgaacctc tggaaaatcc
     9301 tctacaattt tcaggtaaat ttccttggcc tgaggataga aaccaatccc ttcaagataa
     9361 gttgccaact catacagaag atcacttgaa tcattttcta aagctttggc gaaataatac
     9421 tcagccttag ttaaatcttg ctcctccaaa gcctgtaaca tctgttgact attgttcact
     9481 cttgacttcc tcaccctcta attcatatag accactgact accttatacc attcgaaaat
     9541 ggctgaaatg acaaccttag cagaggcata aaccggaata ccaagtaaaa ctccccagat
     9601 accaaacata gatcctgaag ttaacaaaac aaagagaaca ttaatagggt ggatgtttaa
     9661 ttgacttccc aaaatcaatg gagagacaaa acggccttca atagtttgtt ctacgataaa
     9721 gacaatcact actttcaaaa gcatgactgg accagcaatc aaacccaata ctagagcagg
     9781 aagcatggct agaaagctac caagataagg gaccagattt aaaataccag cagtaacccc
     9841 cagcgtaacc gcatagcgta gaccaataat cttgaagaag atgataaata ttactgctac
     9901 aataatagcc actgtcactt gccctcgaac atagttggac aactgttgat tcacatctga
     9961 taaaacttgt ccaacaggtt ccttcaattt tcttggaatg aattgggtca aatagttacg
    10021 caagcctttc ccatcacgca agagataaaa gagcatgaaa ggaacgataa tcaaggcaac
    10081 aatcacttga gaagctccac taataaaggc actcacccag ttgactgcct gagatgaaac
    10141 cttacttgcc aaaactgtag cctggctaga aaaattggtc aaaacttgct ctaattgagg
    10201 tctgaaatca tctggcaggt gctgggctac caatccatta acaatcctat ctatatcttc
    10261 taagtaaaca ggaacgtttc ttgcaaaggt caaaacctga cgttgcagat ttggaatggc
    10321 gactgccaag ccccaaatga taaagagagc gatgataaca aagacaatag tgatagctat
    10381 aacacgatta accttatgct tctccatcca atcaacaata ggattcaaca aataatataa
    10441 caaaccagac aaaatgactg gcaacatcac aactgctaaa aaatctaaaa caggtgaaaa
    10501 tagaaaacta atcttactta aaataaaaag attcaatccc aataataagg ttactaaaaa
    10561 taccgtaatt gccttgttat ctaaaaacca cttgaaaaac caagataggc taaaatgttt
    10621 ctctttttgc tccataaata cttcctttct attgttctct cattatacca tttttgaaac
    10681 aaaataactc ttttttaaag tgcttacatc taattgaatg tctttaattt gcttgttttt
    10741 gtgatataat agtcttatct ttacacagag gtatagtaat gaaagaaact gtttattttg
    10801 gaacttatac acgtcgtact tctcaaggga tttacaaggc agactttgat acagaaactg
    10861 gtcagctttc aaatctagaa ctttttgcag ctgagccaag tccaacctac cttgcctttg
    10921 accagcacca acatttatac actgttggta gccaagacga taagggggga attgcagcct
    10981 atcaaactga cgggactgtg ttaaatcatg ttgttgaaga aggagctccc cactgttatg
    11041 ttgctgtcga tgaaaagcgt gatttggttt acgcagccaa ctatcacaag ggacaagtcc
    11101 ttgtttataa acgccaggaa gatggtagtc ttctacttag tgatatggat caacacagtg
    11161 gccaaggtcc acatgaaaat caagcttccc cccatgttca ctatacagat ttaacacctg
    11221 accactatct agtgacctgc gacttgggaa ctgaccaagt catcacctat gacctcgatc
    11281 aagaaggaaa attatctaag ctctatacct atcacagcaa gccaggagca ggctcacgcc
    11341 atatcatttt ccataaccac tataaaatcg cttatctcat ttgtgaactc aatagtacta
    11401 tcgaagtttt aatctacgat ggcgttggcg aatttgtacg tatgcaggtt atttcaactt
    11461 taccagaagc ttacgaaggc tttaatggaa ccgctgctat tcatctctct aaagacggta
    11521 aatacctcta cgcttctaac cgtggccatg attctatcgc agtatatacc atccttgcgg
    11581 acggtagctt agagttatta gaaatcgttc caacgcatgg tcagactcca cgtgattttg
    11641 atttgacacc cgaccaaaaa tttctcattg ttgtccatca agactctgac aatgcaactg
    11701 tctttaaacg taattgtgac aatggtcgtc tagcagaact ctccaacgaa ttccatgttc
    11761 ccgaagcagt ctgcatccgt tttgctcctt aaaacaccat aaaaaa
//
LOCUS       DAVLVF010000051        11772 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVLVF010000051 DAVLVF010000000
VERSION     DAVLVF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11772
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.051"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..323)
                     /locus_tag="VYN66_001373"
     CDS             complement(<1..323)
                     /locus_tag="VYN66_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEV1495386.1"
                     /translation="MSVLDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARY
                     REIILAEHDSCIKNDEAVRNFVTSVLLSAFVSAMVPAMISLEIQTYKFVIPFIIGMIW
                     TVVVFL"
     gene            complement(325..2148)
                     /gene="dnaK"
                     /locus_tag="VYN66_001374"
     CDS             complement(325..2148)
                     /gene="dnaK"
                     /locus_tag="VYN66_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV1495387.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKTKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(2628..3152)
                     /gene="grpE"
                     /locus_tag="VYN66_001375"
     CDS             complement(2628..3152)
                     /gene="grpE"
                     /locus_tag="VYN66_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV1495388.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(3179..4213)
                     /gene="hrcA"
                     /locus_tag="VYN66_001376"
     CDS             complement(3179..4213)
                     /gene="hrcA"
                     /locus_tag="VYN66_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV1495389.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(4377..4886)
                     /locus_tag="VYN66_001377"
     CDS             complement(4377..4886)
                     /locus_tag="VYN66_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV1495390.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCIATFLGGLVVCMSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            5494..7827
                     /locus_tag="VYN66_001378"
     CDS             5494..7827
                     /locus_tag="VYN66_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1495391.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAMVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            7840..9303
                     /locus_tag="VYN66_001379"
     CDS             7840..9303
                     /locus_tag="VYN66_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1495392.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            9303..10871
                     /locus_tag="VYN66_001380"
     CDS             9303..10871
                     /locus_tag="VYN66_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1495393.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLSEQQRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNS
                     YYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLAR
                     FIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHVTVIRV
                     LSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIV
                     DKIEQFFAHIDALI"
     gene            10928..11725
                     /gene="psrA"
                     /locus_tag="VYN66_001381"
     CDS             10928..11725
                     /gene="psrA"
                     /locus_tag="VYN66_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV1495394.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
BASE COUNT         3764 a         2108 c         2258 g         3642 t
ORIGIN      
        1 agaaatacaa ctactgtcca aatcatacca attatgaacg gtattacaaa tttatatgtt
       61 tgtatttcta atgatatcat agctggtacc atcgccgata caaatgcaga caacaatact
      121 gaggtaacaa aattccttac cgcttcatca tttttgatac aactatcatg ttcagctaat
      181 ataatttctc tatatctagc taaatattct attggaatat tatcaataaa atcttttgat
      241 gttctatagt tatcagcttg attacaaaaa tcattataaa cttttcgtgt attttttaga
      301 tactcttcat ccaatacact catcttactt ttccgtaaac tctccgtcta cgacgtcatc
      361 gcctgcgttc cctgttgctt gtgcgccttc tgctccttct tgagcttgtt gcgctgctgc
      421 ggcttgttcg tagagtttaa cagcaagtcc ttgagctttt tcgttcaatg cttcaagttt
      481 tgttttcatg tcgtccaagt tgttgtcttc ttgagctttc ttaaggtcat caagggcagc
      541 ttgggcagcg tcacgttctg cgtcgaagcc tttaccttca gtttccttga ttgtcttttc
      601 agtcgcaaag attgcttggt ctacttcatt acgaaggtct acttcttctt tacgtttctt
      661 atcggcttca gcgtttgctt ctgcatcttt catcatgcgg tcgatttctt cgtcagtcaa
      721 acctgagttc gattggatga caatagtttg ttctttttga gttccaaggt ctttggcctt
      781 aacagacacg ataccgttct tgtcgatgtc aaatgttact tcgatttgag gaattccacg
      841 aggtgcagct gggatatcag tcaattggaa gcgtccaaga gtcttgttat ctgctgccat
      901 tgggcgttca ccttgaagaa cgtggatatc aacggctggt tggttgtctg ctgctgttga
      961 gaagacttgt gatttagatg ttgggattgt agtgttgcga tcgataagtt ttgtaaatac
     1021 tccacccatt gtttcgatac caagtgacaa tggcgttaca tcaagaagga caacgtcctt
     1081 gacatcacca gtaatcacac caccttggat agccgcaccc atagcaacta cttcatcagg
     1141 gtttactgat ttgtttggtt ctttaccagt ttcagcttta acagcttcaa caacggcagg
     1201 gatacgagtt gaaccaccaa caaggataac ttcgtcgatt tctgacaagc tcaaacctgc
     1261 atctgaaagg gcttgacgaa ctggaacttt tgtacgttca acaaggtcac gagtcaaatc
     1321 gtcaaatttc gcacgagtca aagtcatttc caagtgaaga ggtccagcct cacctgcagt
     1381 gataaatggc aagctgattt gtgttgaagt tacaccagaa aggtctttct tcgctttttc
     1441 agccgcatct ttcaaacgtt gcattgccat cttgtcagta gacaagtcga taccgttttc
     1501 tttcttgaat tctgctacca agtggtcaat gattttttgg tcaaagtcgt caccaccaag
     1561 tttgttgtcc cctgcagttg acaatacgtc gaagacaccg tcacccaatt caaggataga
     1621 gacgtcgaat gtaccaccac caaggtcaaa taccaagatt ttttcttctt tgtcagtctt
     1681 gtccaaacca taagcaagag ctgctgcagt tggttcgtta acaatacgtt ctacttcaag
     1741 accagcaatt ttaccagcgt cttttgttgc ttgacgttga gcgtcgttga agtaagccgg
     1801 aactgtgata acagctttgg ttactttctc accaaggtag tcttcagcgt agcctttcaa
     1861 gtattgaagg atcatagctg agatttcttg tggagtgtat tcttttccat ttgcagaaac
     1921 tttttcagaa gttcccatct tagatttgat agagataact gtatctgggt ttgtaactgc
     1981 ttgacgtttt gcagcatcac caacgatgat ttctccgttt ttgaatgaga ctacagatgg
     2041 agttgtgcgg tttccttctg ggtttgcgat gattttgctt tcagttcctt caagaactgc
     2101 aactgctgag tttgttgtac ctaagtcaat accgataatt ttagacatgt gtttttctcc
     2161 ttgttagtta attttctatt tttctttttg atactcttct taagtggcga cgccgtcaga
     2221 gcttgacttc gtcagtctct tttactaaac tttgagccta aggtctcaaa atttgcgcaa
     2281 atagcgccac ggcgaagagt atcgtctttg gttcgcttcg ctcactatta caaagcaaaa
     2341 catattttat ctttcttcat agtttattgt cgtttcggac aagttttcta ttatgtaaat
     2401 tgcgacacga ggagtcgaaa tcgattttat ttcgacgacg agttagtaag gaagctaggc
     2461 aaacgccata gcaattgccg ttttctgacg agttgcttta gctaccgtca gaattgccga
     2521 atcgaacacg ccctaagctc tgagtaaaaa agatagattt tcttgtgttc atcgaacact
     2581 tcgtcaatct cctattttta ctgcgagctt tttacgggct ttgtatctta gttatacacc
     2641 actaccattg ctgggcgtag gatgcggtca tggagtttgt agcctttttg aaagacttgg
     2701 gcgatggtat ctactgggtg ttcatcgtct gctgggagag tttggatggc catatggtag
     2761 ttatggtcaa attcgccatc tgctgcgatt tcttcaattc cttcttcttt caaagcgtga
     2821 atcaagcttt cttgcaccat ccccaagccc ttcttcacat catctgtcaa accttcaact
     2881 gcaagtgcac gctcaaggtt gtcaagagat ggtaagattg cttttgccaa gtcctggcta
     2941 cgataacgtt gcaagttttg acgttcttca ttggcacggc gttggatatt ttgcatttct
     3001 gcatgagcgc gaagatattt gttttcgaac tcatctgcac gttcatttgc caagtccaac
     3061 tcagactttt caggagttgt ttcttcagct gtttccacaa cttcctcttc ttgaacttct
     3121 tctacttctt cattttttat atcttgggcc atgttcgcct cctttaatga tttcaatctt
     3181 aatgtacttc gtaatgatta ctgctgaggt agcggtaaaa atctgtcaac ttcatggtca
     3241 aaacacggtt gaccacattg atttgattga ttagctgttg gtaatccaga ttaactggac
     3301 cgataatggc tagaattcca actccccgat aaggaatgag gaacttacta ctaatcaccg
     3361 ctaggtcagc taaacaggac tcttgaccgt ctgcaacacg aacattttgc atctgatcct
     3421 cacgcaaccc ctcacgaatc tccaaggcca ccttttgcgg ttggtcaaag aactgatagg
     3481 ctgctagatt ggcaaaattc aagagattga ccttgcccgc catcacaatg ttttcgttga
     3541 acatttcctt aaagatgtgt tcaaagagat cgatgacatt atccgttgtt gtaaagtaac
     3601 gctggataat ctgcggaatc tccgtccgaa tcttgtagtg aatatctaaa acggtgtgac
     3661 cgaggaaacg ttcctgaatg atgctcttca gtttcagcaa atcctcctgc aagaagttcc
     3721 ttggaatcag aaactgacta gtaaccgttc gcgactcgtc tagggtaaat accgccaagg
     3781 ctgtatgttg ccccaaaaca acgatatcaa aggctgtcaa acgttgcctg ctcggctcaa
     3841 catccagtgc cactaccgta cagccactca ggtctgttag caagttagca gcctcttgca
     3901 gaatatcctc caatttgaag aattcctgat caaaggcttt gacaatctca tatacctcat
     3961 tttcagccag tcggtcaaaa tccagtgagt gtttcacata gtactgaaaa ccagcaacac
     4021 ttggcatccg accgcttgaa gtatgagcct tctcaagcaa cccttgtttt tctagttccg
     4081 ccatgtcatt acgaatggtt gcactgctag agttaataga ctcttgcaag gcttttgatc
     4141 cgacaggttc gtgcgttttg gtaaagatgt caataatcag atttaaaata tcctgctgac
     4201 gctctgtaac catctcatca ctcctctctg tctgatcaat tagcactcaa tgcatccaag
     4261 tgctaactta tgattctatt atacaccctt agaagagaat gtcaagacaa aaactcaaaa
     4321 aattagcact cttttataaa gagtgctaaa aatcaatctg aagacctaga accttgctat
     4381 tcatctactc ggtatttcct ttttagtctg aaataaccat gaattagata agaaatcaga
     4441 aaagcatata aaacaaaaat aagaaagaaa gacatagaaa gatcctctct aaacaaactc
     4501 atacaaacaa caagaccacc taagaaagta gctatacagg aacgaagtct agatcgcata
     4561 acttcccatt tgagcccctt actcatatag acttgatcct ctggaagtaa atcagaagac
     4621 gatttacgaa gaatcgaaca agaacctgct cctatcaatt cccaacctct atctctaaaa
     4681 tcttgcagtt cacgcttatg ctctttaaga aatctagaat catagatacg atagataaca
     4741 tcgtccggtt gaccttgctc aaaatagaac aaaccaaaac gactagttct atacctccaa
     4801 cctttcaaat gcatctcatg taaatattct tctttcttgt ccaaatcaac aatggtgaaa
     4861 atccgaaatt ctactctgct attcattgtc ttaccccaaa attagaaaac atgcctagcg
     4921 ttatttatta gataattctt tccacttttg actcaatctc caaaaaatat aagaaatctg
     4981 aatcgcaaaa actatcaata aaacccaatc tattatgaaa atcaaaaaca cttcccaact
     5041 gaaagaacta cctccagtga caaactttga gaaaaacggt agtagagcta aaaagagaag
     5101 caaaatagga agcatccgca ttgttaaaat ccttttggca taaaaaaatc tttatttaaa
     5161 cgaaaatatt atggcaaaat ttacgccagt ttttgaacgg ctgatgtaga tattttatac
     5221 tttcaaaatg tttaaatgtg attatttatt tttgaaaaat agctcaccag cccgactgaa
     5281 agtgcttata gaatgataat aagtcgcctg ccgaaaacag cgaaaaatag cagtgttatg
     5341 cggagataat ctgacgcgat gcgaaagtat attgcatact tattttcaac aatttagcag
     5401 agtattttta taagtgtgat ataatagaag tataatttgt tctgatagtt tattttatgg
     5461 agaagtagat ttttagaatg cggaggattc aatatggttg agtttataaa gtctaagaaa
     5521 gaaatgagtg aggaggatat taaagcaaat ttcatcactc ctgctattgt atccaaagga
     5581 tggaaaaatg gtgagcatat cgcttacgaa gaatacttca ctgatggtcg aattgaagtt
     5641 agaggagata aggctcgtcg taaagaagga aaaaaatcag actattcact gtattaccaa
     5701 tttggaactc gaattgcaat tgttgaggca aaggataata aacacagcgt tcgagcagga
     5761 ttacaacaag ctattgaata tggagagatt ttagatgttc catttgttta ttcttcgaat
     5821 ggtgatggct ttattgaaca cgaccgtatc acgagagaag aacgtgagct ggagttagac
     5881 gaattcccta ctcgtgaaga attattttct cgtatgacga aggaaaaagg attgacgtac
     5941 gaaattacag aagctatctc aactccatac tatacagacg ccttctcaat gaaaacgcca
     6001 cgctattatc agcaaatagc tatcaaccgt actattgaaa cagttgccag aggacaaaaa
     6061 cgagtaatgt ttgtgatggc aacaggaacg gggaaaacgt tcatggcttt tcaaattatt
     6121 catcgccttc gaaaagctgg tttggctaaa cgagttttat tcttagcgga tagaaacatc
     6181 ttagtagacc aaacgatggc tgaagacttt aagccattcg aaaaggtaat gacgaaaatt
     6241 acaccaaaac ttttgactgc tcctgaaaaa ttaaattctt ttgaaattta tctagggctt
     6301 tatcagcaac taactggtga agatggaact gaaacacatt atcaaaaatt tgataaagac
     6361 ttctttgatt taatcgtaat tgatgaagcg caccgtggtt cagctaagga aaacagtaac
     6421 tggcgtaaga taattgatta tttcagttct gcgacacaga ttgggatgac cgctactcct
     6481 aaagaaacca agaatgcttc caatacggaa tactttggtg agccaatcta tacttatagt
     6541 ttaaaacagg gaatcgagga tggttttttg gctccatatc gtgttatgag ggttaattta
     6601 gatgtggatg tggatggtta tcgtccagaa actggaaaag ttgatactaa cggacaatta
     6661 atagaagata ggtactacgg caggaaagat tttgataaaa ccattgtcat tgatgataga
     6721 acgcaaagag ttgccaagtt tgtttctgat tatatgaagc aaaacaatgc acgatttgat
     6781 aaaacaattg ttttttgtgt tgatattgac catgccgagc gaatgcgtgc tgcacttgta
     6841 aaagagaatc tagacttagt ccaagaagac tatcgttatg tcatgcaagt aactggtgac
     6901 aacgctgaag gaaaagctca actggataac tttatggatg tcaattctaa ttttcccgct
     6961 atggtaacaa cgtctaaatt attaacgaca ggagttaatg ctaaaacatg tcgtttgatt
     7021 gttttagact ctaatatcca atccatgact gaatttaaac aaattattgg tcgtggcaca
     7081 cgtctttatc ctcaaaaggg gaaagaattt tttacgatta ttgattttcg aaatgttacc
     7141 aatttgtttg ctgaccctga ttttgatggt gagccagtga aggtgctgga aacaggtgcg
     7201 aaaacagtca gtggttccac gcccggtttc gtagatgagg aaggtgaccc agtagaaaaa
     7261 tatatcgtta cagacaagca ggttaccatt cttaattcta ctgttcaagt attggatgaa
     7321 aacgggaaac tgattaccga aagcctgacc gactacactc ggaagaatat cttaggtagc
     7381 tacgccactt tgaacgattt tatcacagtt tggcatacgg cggataaaaa gaagcttatc
     7441 ttagacgaac tttataaaaa aggagtttat ctagatgcta ttcgagagtc ggagggaata
     7501 tcagaacaag aaatcgatga ttttgattta ctcctaaaac ttgcctatgg tcaaaaagaa
     7561 ttaaccaaaa cggaacgtat caataagctc aaacaaagcg gatatttata taaatatagt
     7621 gaggaagcgc gtgctgtttt ggaaatttta ctgaacaaat acatggataa aggtattgga
     7681 gaactcgaaa gcattgaaac attaaaactt ccagaatttc agatatatgg tggaaccttc
     7741 aaaatcatca atacttattt tggagataaa aaacgatatt tacaagcaat taaagaattg
     7801 gagcaagagc tatttacagt agcttaatga aaggaaagta tgtcaattac atcatttgta
     7861 aaaagaattc aagatatcac tcgaaacgat gctggtgtta atggtgatgc tcaacgtatt
     7921 gagcaaatgt cttggttatt attcttaaaa atttatgata gccgtgaaat ggtttgggaa
     7981 ttagaagaag acgagtatga gtcaattatc ccagaggaat taaaatggcg aaattgggct
     8041 catgctcaaa atggggaacg ggtattgaca ggcgatgaat tacttgattt tgtcaataac
     8101 aagttattca aagagttgaa agagcttgaa ataacttcaa ttatgcctat tcgaaaaacg
     8161 attgttaaat cagcttttga agatgcgaac aactatatga aaaatggcgt cttgttacgc
     8221 caagtcatca atgttattga tgaagttgat ttcaatagcc ctgaagatcg tcattcgttt
     8281 aatgatattt acgaaaaaat tcttaaagat attcaaaatg ctgggaactc aggagaattt
     8341 tatacgccac gtgcagcgac tgattttatt gccgaagttc ttaacccaaa acttggagaa
     8401 tcaatggcag accttgcttg cggaacagga ggcttcttga cttcgactct gaaccgttta
     8461 agtagtcaac gtaaaactag tgaagatacc aaaaaatata atacagctgt ttttggtatt
     8521 gaaaagaaag catttcctca tcttttagca gttacaaatc tgtttcttca cgaaattgat
     8581 gaccctaaaa ttgttcatgg aaatactttg gagaaaaatg ttcgtgaata tacggatgat
     8641 gaaaaatttg acattattat gatgaatcca ccttttggag ggtcagaatt agaaacaata
     8701 aaaaataact ttccagcaga attacggagt tctgaaacag ctgatttatt tatggctgtc
     8761 attatgtatc gtttgaaaga aaatggtcgt gttggagtta ttttacctga tggttttcta
     8821 tttggtgaag gtgtaaaaac tcgcttgaaa caaaaactgg tagatgagtt caacttgcat
     8881 acgattatta ggttgcctca tagtgtcttt gcaccgtata caggaatcca tacgaacatt
     8941 cttttctttg ataaaacaaa gaaaacagaa gaaacttggt tttatcgttt agatatgcca
     9001 gatggttata aaaatttctc gaaaactaag ccgatgaagt cagaacactt caatcctgtt
     9061 cgtgactggt gggaaaatcg tgaagagatt ctggaaggta agttctacaa atctaaatca
     9121 tttacaccta gtgaattggc tgagttgaat tataatttag accagtgtgg ctttccaaaa
     9181 gaggaagagg aaatcttaaa tccctttgag ttgattcaga attatcaagc ggaaagagca
     9241 actttaaatc ataagattga taatgtatta gctgatattt tgcagttgtt ggaggacaaa
     9301 taatgacacc agaacaactt aaagcaagta ttctccaaag agcgatggaa gggaaattag
     9361 tgccgcaaaa tcccaatgac gaacctgcaa gtgaattatt aaagagaatt aaagctgaaa
     9421 aagaaaaact tatcagtgaa ggaaaaatca aacgagataa aaaggaaact gagatatttc
     9481 gtggtgatga tgggaaacat tatgggaagt ttgctgatgg aagcactcaa gaaattgatg
     9541 ttccttatga tattcctgat acttgggagt gggtgaggtt ttctacattg gttgaaattg
     9601 tcagaggtgg ctctccacga ccaatcaaag attatcttac ttctgaagta gatggaataa
     9661 attggataaa aataggtgat actgaaaagg gtgaaaagta tataaataat gttaaagaaa
     9721 aaatcaaaaa atcagggctt aacaaaacta gatttgtaaa aaaaggtaca tttttgttaa
     9781 ctaattctat gagttttggt agaccttata ttttgaatgt tgatggtgca atacacgatg
     9841 gatggttggc tatttcgaac tatgaaaact cattaaataa agattaccta ttctatattc
     9901 tttcatcaaa tgtagtttat tctcaatttc tatctctaat tagtggagct gttgtgaaaa
     9961 acttgaatag tgataaagtt gcttctattc ttatccctct ccccccacta tccgaacaac
    10021 aacgcataat agaagcaatc gaatcagctt tagaaaaagt agatgaatat gctgaaagtt
    10081 ataatagact agaacagcta gataaagaat ttccagataa actaaaaaaa tctattcttc
    10141 aatatgctat gcaaggaaaa ttagttgaac aagacccaaa tgatgaatca gtcgaagttt
    10201 tacttgaaaa aatacgagca gaaaaacaaa aactctttga agaaggcaag attaaaaaga
    10261 aagatttgga catttctatt gtttcccaag gagatgataa ctcttattat gaggaagtac
    10321 cttgtgaaat accagaaagt tgggaatggg tgaggttaaa cgatataacc tcctacattc
    10381 aaagagggaa gtctccaaaa tattcaaata ttcctatata ccctgtgatt gctcagaaat
    10441 gtaatcaatg gtctggcttt tctattgact tagcaagatt tattgatccc gaaacagttc
    10501 atagttacca gaaagaaaga ttactaagag atggtgattt aatgtggaat tcaacaggtc
    10561 taggtacgtt aggacgatta gctatttacc atgaaaataa aaatccttat ggctgggctg
    10621 ttgcagatag ccatgttaca gttataagag tcttatcggg agttattaat tgccatttca
    10681 tctataattt tctttcctct cctattgttc agtcagttat tgaagaaaaa gcgtcgggta
    10741 gtacaaaaca aaaagaatta ttaacgaaaa ctataaaaga atatttaatc cccctcccac
    10801 cccttcctga acaatccaga atcgttgata aaatcgaaca atttttcgcc catattgacg
    10861 cactaattta gtataatagt tttacgtgcg gtggaatttc tatcaaacat aagataggag
    10921 ttttacgttg aaagattttg aattacattt gaagaaagct ggtttagctg aaaatactgt
    10981 gcgttcgtac ctttatggtg ttcgattttt cttggagaat tacgaactca aaatggaaga
    11041 tttatttgaa tacaaaggat atttattaga caattttaaa cctaaaactg tcaatttgcg
    11101 actacaagga gtaaataaat atctggcttt tattggtcat gatgatttga aattaaaatt
    11161 tgtcaaagta cagcaaaaac catttttaga agatgtcatt agccatgctg attacctttt
    11221 tcttaaacgt agtttgaaaa aagatggtat tttaaaatgg cattttgtcg tttggttttt
    11281 aggtgcaact ggggctcgtg taagtgaact tattaaacta aaagtagagc atgtagaaat
    11341 cggttatttt gacatttact caaaaggtgg taaaattcgt agactgtaca ttcctaaaaa
    11401 attaagaaat agttgtctta gttggttaga atcagaaaat cgccgaagtg gttatttatt
    11461 tttgaataaa tttaacgaac caattacagc aagaggagtt gctcaacagt taaaaaatta
    11521 tgctgataaa tacaaaatga atcctaaagt aatttaccct cattctttta ggcatttatt
    11581 tgctaagaat tttttagcga agtataatga tattgccttg cttgcagatt tgatgggaca
    11641 cgaaagtata gaaactactc gaatttatct aaggaaaaca gctactgaac aacaaaatat
    11701 tgtagataaa attgttaatt ggtaaaaaat aacaggtggt caaactgact acctgctatt
    11761 tttgtgatta tg
//
LOCUS       DAVLVF010000052        11676 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVLVF010000052 DAVLVF010000000
VERSION     DAVLVF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11676
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.052"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            116..919
                     /locus_tag="VYN66_001382"
     CDS             116..919
                     /locus_tag="VYN66_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1495395.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     EVADRIIFMADGEVLVDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            959..1723
                     /locus_tag="VYN66_001383"
     CDS             959..1723
                     /locus_tag="VYN66_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1495396.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQGDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTALLDDSFKPSDYGIVTKKSNT
                     EFNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1736..2413
                     /locus_tag="VYN66_001384"
     CDS             1736..2413
                     /locus_tag="VYN66_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1495397.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2423..3082
                     /locus_tag="VYN66_001385"
     CDS             2423..3082
                     /locus_tag="VYN66_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1495398.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNSVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            3378..4568
                     /locus_tag="VYN66_001386"
     CDS             3378..4568
                     /locus_tag="VYN66_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV1495399.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(4889..5770)
                     /locus_tag="VYN66_001387"
     CDS             complement(4889..5770)
                     /locus_tag="VYN66_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV1495400.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(5911..7239)
                     /gene="vncS"
                     /locus_tag="VYN66_001388"
     CDS             complement(5911..7239)
                     /gene="vncS"
                     /locus_tag="VYN66_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV1495401.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD"
     gene            complement(7236..7892)
                     /gene="vncR"
                     /locus_tag="VYN66_001389"
     CDS             complement(7236..7892)
                     /gene="vncR"
                     /locus_tag="VYN66_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV1495402.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(7941..8024)
                     /gene="pep27"
                     /locus_tag="VYN66_001390"
     CDS             complement(7941..8024)
                     /gene="pep27"
                     /locus_tag="VYN66_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV1495403.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(8204..9583)
                     /gene="vex3"
                     /locus_tag="VYN66_001391"
     CDS             complement(8204..9583)
                     /gene="vex3"
                     /locus_tag="VYN66_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV1495404.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(9635..10282)
                     /gene="vex2"
                     /locus_tag="VYN66_001392"
     CDS             complement(9635..10282)
                     /gene="vex2"
                     /locus_tag="VYN66_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV1495405.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(10295..11572)
                     /locus_tag="VYN66_001393"
     CDS             complement(10295..11572)
                     /locus_tag="VYN66_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1495406.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGNSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         3271 a         2613 c         2228 g         3564 t
ORIGIN      
        1 gatcgatttt tcctgttctt atttcactat agtgaatttt ttcagatatg gtttgacaag
       61 aacattcttt tattgcagaa ttgtgttata aaatctaaca taaaggagtt ctctcatggc
      121 tttagtagaa tttaaaaacg tcgaaaaata ttacggagac taccacgcac tccgcaacat
      181 caatctccgt tttgaaaaag gacaagttgt tgtcctgctt ggaccttctg gctctgggaa
      241 gtccactctt atccgtacga tcaatggttt agaggctgtt gacaaaggaa gtctcctagt
      301 caatgggcac caagttgctg gtgccagcca gaaagatttg gtacctcttc gcaaggaagt
      361 cggcatggtt tttcaacatt ttaaccttta tccacacaaa acagtgttag aaaacgtgac
      421 acttgcaccc attaaagttc taggaattga taaaaaagaa gctgaaaaaa ccgccaaaaa
      481 atatctggaa tttgtaaata tgtgggacaa gaaagattcc tatcccgcca tgctatctgg
      541 tggacaaaaa cagcggatcg ccatcgctcg tggtcttgct atgcatccgg aactcctcct
      601 ctttgatgaa ccaacatctg ctcttgatcc tgagactatc ggagatgttc tagcagttat
      661 gcagaaactg gcgcgtgatg ggatgaacat gatcatcgtt acccacgaaa tgggctttgc
      721 tcgagaggtt gcggaccgca ttatctttat ggccgacgga gaagttttag aggttgcgga
      781 ccgcattatc tttatggccg acggagaagt tttagtagat acgacagatg tcgataactt
      841 ttttgacaat ccaagcgaac ctcgtgccca acaattcctc agcaaaatta tcaaccacga
      901 aagtgacaaa gtcaaataag gaggcgccta tgaaaaaaaa ttctttttat cagcattatt
      961 gattagcctt ttcggccttg ctgctgccaa accagtccag gctgatacaa gtatcgcaga
     1021 cattcaaaaa agaggcgaac tggttgtcgg tgtcaaacaa gacgttccca attttggtta
     1081 caaagatccc aagaccggta cttattctgg tatcgaaacc gacttggcca agatggtagc
     1141 tgatgaactc aaggtcaaga ttcgctatgt gccggttaca gcacaaaccc gcggccccct
     1201 cctagacaat gaacagggcg atatggatat cgcgaccttt accatcacgg acgaacgcaa
     1261 aaaactctac aactttacca gtccctacta cacagacgct tctggatttt tggtcaataa
     1321 atctgccaaa atcaaaaaga ttgaggacct aaacggcaaa accatcggag tcgcccaagg
     1381 ttctatcacc caacgcctga ttactgaact gggtaaaaag aaaggtctga agtttaaatt
     1441 cgtcgaactt ggttcctacc cagaattgat tacttccctt cacgctcatc gtatcgatgc
     1501 cttttccgtt gaccgctcta ttctatctgg ctacactagt aaacggacag cactactaga
     1561 tgatagtttc aagccatctg actacggtat tgttaccaag aaatcaaata cagagttcaa
     1621 cgactatctt gataacttgg ttactaaatg gagcaaggat ggtagtttgc agaaacttta
     1681 tgatcgttac aagctcaaac catctagcca tactgcagat taaggaggac accccatgac
     1741 agatttatca tcttggacag cctattttca ggattttgga caatttttca atggtttcct
     1801 cttcaccctt gccctagcga ttggatcctt tatcctcgcc atggtcttag gaatcttctt
     1861 tggtgccatg tcaaccagta aacgtccaat tttacgcatt ttagctcgca tctttgtgga
     1921 attttaccaa aacactcccc tcttggtgca gtttgttatt gttttttatg gcctacctct
     1981 tatcagtgac catactatca tgattccgat ttattggaca gctgttctct gcgtgggact
     2041 ctatcacggc gcttatatcg ctgaggttat tcgttcaggg attcagtcta ttcctagtgg
     2101 tcagatggag gccgccttat cgcaaggttt tacctatatc agtgccatgc gcttgattat
     2161 cttgcctcag accttccgca tcattctccc tccattgacc aaccaaattg ttaacctcat
     2221 caagaacacc tctactgtag ctatcatctc tggagtagac ttgatgtttg tgactaagtc
     2281 ttggtcggct ctcaacggaa actatatccc agccttttta ggtgctgctc ttctctactt
     2341 tgccctatgc ttccctgttg cccagtttgg tcgcaagatg gagcaagcca acaaaaaagc
     2401 ctattcactt taggaggtta ctatggaatc tattttagaa gttttaaccc cagataacct
     2461 agtctttatc tttaaaggat ttggcttgac cctctatatt tctctgattg ccatcatcct
     2521 ctctactatc atcggtacgg tgctagctgt catgagaaat ggcaaaaatt ctgtcttgcg
     2581 aatcatctcc agtatttata tcgagtttgt gcgcaacgtt cccaaccttc tctggatttt
     2641 tactatcttt ttggtgttca aaatgaaatc cacaccagca ggtattacag cctttactct
     2701 ctttacatca gcagccttgg ctgagattat tcgaggcggt ctcaatgccg tagacaaggg
     2761 acaatacgaa gcaggaatgt cacaaggctt cacctcagcc caaatcctct actacatcat
     2821 tctcccacaa gccatccgca aaatgctacc agccatcatt tctcagtttg ttaccgtgat
     2881 taaggatacc agtctcctct actctgttat cgccctacaa gaactctttg gagccagcca
     2941 aattctcatg ggccgttatt tcgaaccaga gcaggtcttc agtctttaca tcctgattgc
     3001 cctcatctac ttcagcttta acctagcaat ttctagcctg tctcatatgc tagccaaacg
     3061 ttggcaacaa gccgcagaat aatactcttc gaaaatctct tcaaaccacg tcagcttcac
     3121 cttgccgtat atatgttact gacttcgtca gttctatcca caacctcaaa acggtgtttt
     3181 gagcagcctg cgggctagct tcctagtttg ctctttgatt ttcattgagt ataaacagcc
     3241 actctccaat tcaattggag agttttttga tgagagtaga tatttataga cctcaatttg
     3301 acaccataca ttttctatga taaaatgtaa caaattcatt tacaacaaat cgaaatctaa
     3361 atgaaatgga attttctatg aaatcagtca aaggactact ctttatcata gctagtttta
     3421 tcttgactct tttgacttgg atgaacactt ctccccaatt catgattcca ggactagctt
     3481 taacaagcct atctctgact tttatcctag ccactcgtct cccactacta gaaagctggt
     3541 ttcacggttt ggagaaggtc tacaccgtcc acaaattcac agcctttctc tccatcatcc
     3601 tactaatctt tcataacttt agtatgggcg gtttgtgggg ctctcgctta gctgctcagt
     3661 ttggcaatct tgccatctat atctttgcca gcatcatcct tgtcgcctat ttaggcaaat
     3721 acatccaata cgaagcttgg cgatggattc accgcttggt ttacctagcc tatattttag
     3781 gactctttca catctacatg ataatgggca atcgtctcct tacatttaat cttctaagtt
     3841 ttcttgttgg tagctatgcc cttttaggct tactagctgg tttttatatc atttttctat
     3901 atcaaaagat ttccttcccc tatctaggga aaattaccca tctcaaacgc ttaaatcacg
     3961 atactagaga aattcaaatc catcttagca gacctttcaa ctatcaatca ggacaatttg
     4021 cctttctaaa gattttccaa gaaggctttg aaagtgctcc gcatcccttt tctatctcag
     4081 gaggtcatgg tcaaactctt tactttactg ttaaaaattc aggcgaccat accaataata
     4141 tctatgataa tcttcaagcc ggcagcaaag taaccctaga cagagcttac ggacacatga
     4201 tcatagaaga aggacgagaa aatcaggttt ggattgctgg aggtattggg atcaccccct
     4261 tcatctctta catccgtgaa catcctattt tagataaaca ggttcacttc tactatagct
     4321 tccgtggaga tgaaaatgca gtctacctag atttactccg taactatgct cagaaaaatc
     4381 ctaattttga actccatcta atcgacagta cgaaagacgg ctatcttaat tttgaacaaa
     4441 aagaagtgcc cgaacatgca accgtctata tgtgtggtcc tatttctatg atgaaggcac
     4501 ttgccaaaca gattaagaaa caaaatccaa aaacagagct tatttacgaa ggattcaagt
     4561 tcaaataagt aatcatactc tcctgggatt tcccaggaga gttttttata gccaaatgaa
     4621 ataagaccgg aacgatggaa ttttggcatt caaatcaatt gataagaata ttttatagtg
     4681 gagtgaaata ggatacgaac aaattgatta ggaaaatcaa atgaatttat agaaatcttt
     4741 tagcagttat gttgtcctat tctagtttca aaacgctata gaagcagcat tgtactagtc
     4801 tagattcagt ttactatact aaaacgagta gcttgaaatc aaaaaaccca ccctcacagg
     4861 caggttttat ctgtattatt cagctagatt atgctttacc ttctgaaccg aatacgtcga
     4921 tacgttcttc aaccgatgct tggatagctt ttacaccgtc agccaagaat ttacgtgggt
     4981 cgaagagttt tttcttgtcg tattctgctt cgtttgcttc gtagtcacga gcaaatttac
     5041 gagttgcgtt agcgaatgcg atttggcatt ctgtgttaac gttaactttg gcaacaccaa
     5101 gtttgatagc tgcttggatt tgctcatcag gaatacctga tccaccgtgc aatacgattg
     5161 ggaatcctgg aagagcttct gtcaattttt gcaagtggtc aaggtcaaga ccttcccagt
     5221 ttactgggta agggccgtgg atgttaccga taccagctgc caagaagtcg ataccagttt
     5281 caaccattgc tttagcgtct tcgattggag ccaattcacc tttaccgatg attccgtctt
     5341 cttcaccacc gatagtacca acttcagctt ctactgagat acctttagcg tgtgcttttt
     5401 caacaacttc tttagccaat ttaaggtttt cttcaactgg aaggtgtgaa ccgtcaaaca
     5461 tgattgaagt ataaccaact tcgatacact caagtgcatc ttcgtagtga ccgtggtcaa
     5521 ggtggatagc tactggtaca gtgataccca ttgattcaac aaggttagcg atcaagttgc
     5581 gagcaacttt gtaaccaccc atgtatttag cagcacccat tgaagtttgg atcaaaactg
     5641 gagctttttt agcttctgct gcgcgcaaga tagcttgagt ccactcaagg ttgtttgtgt
     5701 taaatccacc aactgcataa ccgttgtcac gggctgcttg gacaaatttt tctgctgaaa
     5761 cgattgccat tttatcaggc ctcctgtata tttttatggg tcatcccatt tacattgttc
     5821 attttatcac tttttgccaa aaaaatctag tttttcccgc agtttcgatt gattttcttc
     5881 taactccatc tatgtaaacc ctttctctcc ctagtcttgg gcgacttttg gaaaagctat
     5941 aaagaaggtt aaacgattct cctccatctc gaaacgataa gctaattttt catgttctaa
     6001 tagactctta accacaaaga gccccatacc agaccccttg accttgcgac tggcattgtc
     6061 agaaaaagac tgggctagtt tttcttgttc ctctgagcta cagctatttt cgataaaaag
     6121 ttctccttct ctttctccaa ttcgaactaa gccacctgga acagagtgct taatggcatt
     6181 gctgatgaga ttagaaagaa tcaacttcat aactgatggg tttagataag cctgctgatg
     6241 ggtcaaacta ttgtctatct ggagctctct ttccttggct agcaaggcat aatctttgac
     6301 cagattttgc gtcatctgga ggaggtcaat tgtttcccta tcatctcgca attcctgcac
     6361 agaagagagg gaaagtatct gcagaacatg gtgattgagt tcatccacaa tccccaaggc
     6421 aactcccaga tactggtctc tatccttata acgaccgata ttctctctca tattttcgat
     6481 taggattttc aaactagcca gcggtgtttt caattcatga gaagctcctc gtaggaattc
     6541 gaccttcatc ttctccagct ggagaatggc ttcattcttt tcatgcaagt ccgcaataac
     6601 agtcaagaga tgctggtaga ggctattgat ttgttccttg agattaccta tctcatcctt
     6661 agaatccacg cgcaatcgca cttgggaatc caggtccatc atccgacggg tcacccgctt
     6721 gatttccaaa atcggtgcaa caatagtccg ggcgtagatg taggccacca agagggaaat
     6781 cagaaaggag gccagcaagg tatagggaag aaactggaga ctgatttgct ccgcttcctt
     6841 ttgtaaatcc atggaagcta gaaactggag aatcatagta ccaccgtctt gcgttttcac
     6901 ctcgcgctcc tcaataaaga gagaggttgt ctggcggtct gtgtccagag gaagactgtc
     6961 cttgacttct aacttgtcct cggtcatctc acctttgacg gtccccttga tatcactagt
     7021 ctgggaatac aagtctaaca cttgctcgat actctgccta tctttccctt ctagggactg
     7081 ggcaatggct gttgcctttt gaccaatggt ttcctgacga tgactcagat aagtcgaagg
     7141 aaaaagaaaa taaatagcta aatgaaggca gataaccaga acactaaata tcgagaaggt
     7201 atagataaat atctttgcaa ataaacctgt tcgtttcatt ttcgctccaa tttataacca
     7261 acattgcgca cagtgaggat acaatccaag tctagctttt tccgcaattc cttgatataa
     7321 acatcaataa cacggtcaaa gggaacctca tctgtcgctt tccagacggc atcgataatc
     7381 tgagaccgag tcaaggcccg gccttcattt ttcactagat agtccagaat ttccaactct
     7441 ttggcattga taggcacttc ttgacctgcg aggcttgcac tgtagctttc aaagtccacc
     7501 ttggtatcct tgtaagaaaa gattcgtcct gtatcgtagt agcgcttgaa aatcgcgtcc
     7561 accctcactt ttaagaggga gagagagaaa ggtttttcca gatagccatc tgccaaagag
     7621 gcaaaggcac tcatcttgta ttcctcatcc tgaaaagctg tcaacatcaa gacaggaacc
     7681 tgactggttt tacgaatctc agctaggact tctaagccgt taagcttggg catctggata
     7741 tccagtaaaa ccagggccac ctcatagcta gaaaattgct ccagagcttc ctgaccgtcc
     7801 gctgcctcaa tagtttcata gccacaatcc gtcaaataat cactgacccc ctcacggatc
     7861 atctcttcat cttctacaat taaaattttc atactttaac tgctctctat tttttatttt
     7921 tcttagaata aatacctact ctattttcta ttatagtctc ttgctggcct tttgtctgta
     7981 agcaactgat cactagataa aacgttgtga aattcctttc tcataaattc cataacttta
     8041 gtatattata tttaagcact aaagtacaaa gaaagcaact gaaagcaatg attttcacca
     8101 ctgctttcag atttattttg aattgttaaa tagctattcc tatccactat tcttgaatag
     8161 aaacacaaga tgcaatcttt attccagact cattttttca aatttattca ccatccagca
     8221 agagctcttt tggttgtttt ctaaggagat tgcttgaagc aagcgccata acgagaacca
     8281 ctagaaccaa ggcaaggaca aaaatgatga taaagtctga tgtctgaatg gaaatgtcta
     8341 ggctcgacaa ggtcttgcta aagccatcta cttctgcacc gccaccaagg ttagaggctt
     8401 gagccgcctt actagcctgt ttggcaacac ctgaagtcac attggcaagg acagtgtttc
     8461 caattgcacg ggcagtgtaa ttagctagga agtaagcaga aactagagca gggatagcaa
     8521 tcaagataga ttcggtgatg aattgaccca agatacttgc ctgcttgagg ccgatagaga
     8581 ggagaattcc cacttccttg cgacgggcgt tgatccaaag gctgagcaag agggcaagga
     8641 ggagaactga gaagctcaag ctaccccaga agaggaggtt ggccatcttg tacataccag
     8701 agatagattg ctcaagagct gggtagttag aggagctctt gacgagtgtg tagctcttcc
     8761 agttgatacc actgatgcca ttcaactctt tcataacatc atccaagttc ttgtctgctg
     8821 ttacaaagaa ggttgcgtcc ccataaatgg ctgtgtcttc tgtgtatcca taaagttttg
     8881 cagcagtgtg aatgtctgta atagctgtgt tttcgtaaag ttcttgtgag taggttactg
     8941 ctgacttatt atgaccatca aagagtccct tgattgtcac ttcaactgtt tccttggctc
     9001 ctttttcatt atctgcatcg tagatattag agtccagttt aaccttgtcc cctactttcc
     9061 agccgtgttt ggctgccaag tccttgtgca agaggatttt atccttgtcg tcgttggtta
     9121 agtgctctcc ttcgactagt ttataagaac cagagacaaa cttgtcttct ttagaggagt
     9181 cattgacacc tgtaatcatc aagctacttc caaaacgttt ggcacgatca gcagtgagat
     9241 tcttcttggt ttctggcgtt tcaatcaggt catatccagt caaatctccg atagcgttga
     9301 tacgtttgac ataagactca atggccttgt tttcggtgat ttttttgatg tcttcaccct
     9361 tgatattccc agcaccgcga ggcgttcctt ggttgacgcg acgattgatt tgcatggaga
     9421 agctattggt gatattttta aaggtctcct gagaagcctt ggcagtagct cccttgattg
     9481 acaagccgac caaactcaag ctcgccatga ggagaataat caggaagatg acaatcgatt
     9541 tgaaaaactt ccttgtaaca taggcaaatg cgttgtgtaa catagattcc ctttctagat
     9601 tttgttttaa tcattctatt aaagtaagct caaattattt actagtattg cgcgtttcag
     9661 tcagtttctt atcctttaat tcaagtgtaa tatctgacgc ttgtgccact tctttactgt
     9721 gagttacgac aatcacacat ttacctgttt tctgggcaag tgatttgagt agttcgacaa
     9781 tatctccagc agttttagga tccagatttc ctgttggctc atcagctaga ataactggag
     9841 cttctgagac caaactgcga gcaatggcaa cacgttgctg ttgaccacct gataactgga
     9901 gaacattccg cttgatctgg ctttcatcca aaccaagctc aagaagtgta ttcttgcttg
     9961 cctttttgtt gaccaatcgg atattttcca gcggagaaag ataatctatc aagttataat
    10021 tttgaaagac cagggaaata tggtgcatgc gatggtaaga atagcccttc ttacgaatat
    10081 cctctccttg aaaaaggata gaaccttcaa caggactatc tagaccagca agtagggaca
    10141 agagtgtgga ttttcctgct cctgactccc caataatact gtaaaatttt ccgggttcaa
    10201 aattataatt gatctgatat aggactgctt cagcagtatt cttataacgg taggtaacat
    10261 cttgtaattg taataaagtc atgatttctc cttcttaact aatagatgat aaaatttctt
    10321 tcggtgattt tctaaataag aataggaaac aaagggctac agataagcaa ctaagcagaa
    10381 ctagaaaaac ataggattct gcaaaagata agatgctagt tgataaactg cttgctttgg
    10441 ctagtgtatc ttgtaagctt gcctgatctc cacttgctag tagagtttgg agtaggtaag
    10501 ttgtgattcc gtttcctgca acaaatgctg gaagcaaagc tccaagagat accaaaacta
    10561 cctctaaaca gaattgtagg aaaatcgagc tcttgccttt tccaagtgca agtaaaatcc
    10621 ccacttcata gacccgttct ctcaaccaga gagacaaaac cagaattaag gctccagctc
    10681 ctgctatcaa catcccataa aggaagatgg tcaggaaggt ttggaaagtt gcaactgagt
    10741 ctttgatttg ttcaaaagcc ttgttttcct tttcgacttg gtagccttga ttttccaagg
    10801 ccaagttttc tacctgcttc atgagtccgt ccatttcctt aggattttct acatagaagc
    10861 gtgctgcact gacttgagct tcactattgc ccaaaagggt ttggctactt tcatagtctg
    10921 taaagacttg attttcactg aagtcagaag acaagcctgt gaatttctct tgttttttac
    10981 cagaaaagat gccgataatc tcaaactcta ctgtttgtcc ttttccagat tcagactgac
    11041 cagcatccaa gccaatcttg tcatgaagtg aaagaccgtt cttcttagcc aattcttcgt
    11101 ggataaggat tttcttggaa ttcccttttt gaaggtgtcg cccttctttt agattgaaag
    11161 ccgaactggt aaaggttaca tccttggatg aatcctcaag agccgttaag ctaaccaagt
    11221 tattgtctgc agctgataaa tcatcacgct ccacgctctg ctcgccagtc actgcttcct
    11281 tgtcttttag ttttgcgacc gtctcgagtt caggagagac attttccagc cccttaatct
    11341 tgcttacaga tgctaggtct gacaacttga atgtctgacc attctctatc ttcttaatag
    11401 aaaaagatgt attgagtgat ttataaagat tgctttctac tgttttgttg gacttcatca
    11461 gagtcaaaca ggctgaaatt ccggccaata agaccaataa aatcagaaat aaaataaaac
    11521 ttctcagtcg ctttctgctg acataagccc aagatctttg gattggattc atttgtcacc
    11581 tccatatttg taagactatt ataaaaccca aatatgaaat gtttatgaaa tacgaaaaaa
    11641 aaatatcgag taggggataa tctctagccc ctctca
//
LOCUS       DAVLVF010000053        11615 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVLVF010000053 DAVLVF010000000
VERSION     DAVLVF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11615
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.053"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(141..992)
                     /gene="rgg"
                     /locus_tag="VYN66_001394"
     CDS             complement(141..992)
                     /gene="rgg"
                     /locus_tag="VYN66_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV1495407.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNVAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFYTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            1116..1457
                     /locus_tag="VYN66_001395"
     CDS             1116..1457
                     /locus_tag="VYN66_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1495408.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            1515..1595
                     /locus_tag="VYN66_001396"
                     /pseudo
     CDS             1515..1595
                     /locus_tag="VYN66_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(1711..3414)
                     /gene="ilvD"
                     /locus_tag="VYN66_001397"
     CDS             complement(1711..3414)
                     /gene="ilvD"
                     /locus_tag="VYN66_001397"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV1495409.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(3647..4579)
                     /locus_tag="VYN66_001398"
     CDS             complement(3647..4579)
                     /locus_tag="VYN66_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV1495410.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(4576..5433)
                     /locus_tag="VYN66_001399"
     CDS             complement(4576..5433)
                     /locus_tag="VYN66_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1495411.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(5437..6783)
                     /locus_tag="VYN66_001400"
     CDS             complement(5437..6783)
                     /locus_tag="VYN66_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1495412.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(6796..7080)
                     /locus_tag="VYN66_001401"
     CDS             complement(6796..7080)
                     /locus_tag="VYN66_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1495413.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(7084..9114)
                     /locus_tag="VYN66_001402"
     CDS             complement(7084..9114)
                     /locus_tag="VYN66_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1495414.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEYYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            9305..10312
                     /locus_tag="VYN66_001403"
     CDS             9305..10312
                     /locus_tag="VYN66_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1495415.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGVFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            10315..10515
                     /locus_tag="VYN66_001404"
     CDS             10315..10515
                     /locus_tag="VYN66_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1495416.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            10667..10849
                     /gene="rpmF"
                     /locus_tag="VYN66_001405"
     CDS             10667..10849
                     /gene="rpmF"
                     /locus_tag="VYN66_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV1495417.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            10865..11014
                     /gene="rpmG"
                     /locus_tag="VYN66_001406"
     CDS             10865..11014
                     /gene="rpmG"
                     /locus_tag="VYN66_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1495418.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(11506..>11615)
                     /locus_tag="VYN66_001407"
     CDS             complement(11506..>11615)
                     /locus_tag="VYN66_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1495419.1"
                     /translation="TVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT         3759 a         2443 c         1912 g         3501 t
ORIGIN      
        1 tagcacttat gaatttgcga aacgattctt ttgaatatat gcaacaaaca aaatgagcaa
       61 aaaaacgata gaacttttgt aatgacctga gagagggcaa cagttctatc gcttcaaatt
      121 ttttcttggt ttgcagatac tcaacattcg gtcgtatttc tatagtattc ggcagattta
      181 ttacagccaa gcatctcaaa aatacggaca gcatcctcca tctttttctg accttccttg
      241 actctacctt gcttgctatc aaggagacct tctgcccaca gataaacaat tcggaaatag
      301 gtctcatttt ccttgtagaa atgctcttcg ataacacgtt taaaataata ggcattggta
      361 aattcttcac actcaatact agctaaaaag ccattcaata gtatagtata aaaaaggttt
      421 cgattgccag acatttccat tagaaaatcg gatttacgta ccatttctcg tacatatcta
      481 gtaaaaagag aaacagataa aaatggagaa ctgactgaaa ataaattgag ttcatagatt
      541 ccccagatct cggtagaaaa caaataatca tgaaggactt ttccttcctc tgctgttaac
      601 tctacccttt catccatgct cttcatataa gacttgatca taatggcatt tagaatatgt
      661 ttctgtttgt tgtgagaatg ggcatgcttt tgatactccc tgcgatataa gtcctcaaga
      721 ggtgctacat tctttggttc caagacatct gtaatttctt ttctcaactc agaatctgta
      781 tcatactgga aacctctcgc cagaaagagg atctcctcca cactggcaga tatattttcc
      841 agagcaaata gaaacttttc caccgaaagc tcactctgac ctgtttcaaa acgggacaac
      901 atagacggcg aaaattgtcc tccggttgct tgtctcagtg agatatttct tgactctcgt
      961 aattgtctaa agacttttcc aatctgctcc atagacttcc ccttgattcc gtattttctt
     1021 cattttatca tatttttcag aaaattcatc aaaaacttgc caaattgtca gaattatgag
     1081 aaaatagagg atatttatca cgtggaggga ctgctatgag agacgatatc aaaatcaatg
     1141 accgtgcttt ggccttgcaa gaccaaatca tcgaaaaact agagaaagtt tttgatacag
     1201 atgtggaatt ggatgtttac aatctaggtc tgatttatga aatcaatctg gatgaaacgg
     1261 ggctctgcaa gattgtcatg accttcaccg atactgcctg tgattgcgcc gaaagcctgc
     1321 ctattgaaat cgtggcaggt ctgaaacaaa tcgagggtat caaagatatc aaggttgaag
     1381 ttacctggtc gcctgcttgg aaaatcacac gaatcagtcg ctatggccgt attgcccttg
     1441 gactaccacc tcgttaagca ggccaatcat ttttaaagat gaaaaaagca agccaaaaat
     1501 ggcttgcttt ttgatactca atgaaaatca aagggcaaac tagaaaacta gccgcaggtt
     1561 gctcaaaaca ctgttttgaa gttatggata gaactgacga agtcagccca aaacactgtt
     1621 ttgaggttgt ggatagaact gacgaagtca gtaaccatac ctacggcaag gcgacgttga
     1681 cgtgatttga agagattttc aagtatgagt ttatttttta cctgacttgt ccatattcca
     1741 gaagtctgtc acggctccgc gtgaagcaga tgatacgata tgggcatatt taccgaggac
     1801 accacggctg taaagtggtg gcaaggttgt ttctgccttg cgtttttcaa gttcttcttc
     1861 ggatacggcc atggaaattt ctttggtatc ttggtcaacc gtaacgatat cgccggtacg
     1921 gagataggca attggtccac catcctgagc ttcaggagcg atatgtccaa caaccagacc
     1981 ataagtacca ccagagaaac gtccgtccgt caagagggcc accttatctc cctgaccttt
     2041 accaacaatc attgaagaaa gtgacagcat ctcaggcata ccaggaccac ctttaggtcc
     2101 aacaaaacga acaacgacta catcgccatc aacgatttca tctgtcagaa cggcctgaat
     2161 cgcatcttct tctgagtcaa agaccttagc tggcccaacg tgacgacgca ctttaacacc
     2221 tgataccttg gcaactgcac cgtcaggagc aaggttcccg ttcaagatga taagcggacc
     2281 atccgcacgt tttggatttt caagtggcat gataactttt tggcctggag tcaagtctgc
     2341 aaagtcagcc aagttttcag ctacagtctt accagtacat gtgatgcgat ctccgtgaag
     2401 gaaaccattt gccaacaagt acttcataac cgcagggaca ccaccgactt cgtagaggtc
     2461 ttggaagaca tactgaccag atggtttcaa gtcggccaag tgaggcacac gttcttgaat
     2521 cgtattgaag tcctcaagtg acaagtcaac atttgcggca tgggcaatgg cgagcaagtg
     2581 aagagtggcg tttgtagaac caccgagagc catcgttaca gtaatagcat cttcaaaggc
     2641 ttcacgagtc aagatatctg atggtttgag accaagttcc aacatcttaa caacagcacg
     2701 tcctgctgct tcgatatctt ctttcttatc agctgattca gctgggtgag aggatgaccc
     2761 tggcaaactc atccctagaa cttcgatagc agttgccatg gtattagcag tatacatacc
     2821 accacaacca ccagggccag ggcaggcatt acattcaaga cgtttcacgt cctcagctgt
     2881 catgtcaccg tggttccatt ttccgatacc ttcaaagaca gaaaccaagt cgatatcttt
     2941 accatcaaga tttcccggtg caatagttcc accataggcg aaaatagctg ggatatccat
     3001 attagcaata gcaatcatag atccaggcat gttcttgtca cagccaccga tagcgacgaa
     3061 ggcatccacg ttgtgaccac tcatagccgc ctcgatggag tccgcgatga tgtcacgaga
     3121 tgttagagag aaacgcatac caggcgttcc catagcgatc ccgtccgcta cggtaatggt
     3181 tccaaactgt acaggccaag cgcctgcaga tttgacacct tctttagcca gtttcccgaa
     3241 atcatgcaag tgaatgttac atggtgtatt ttccgcccaa gtcgaaatca ctcccacaat
     3301 cgatgtttca aagtccttat ctgtcatacc agtcgcacga agcatagcac ggttaggtga
     3361 tttaaccatg ctgtcataga tgctactgcg gtgacgttta tctaattcag tcatcttatc
     3421 cctcccattt cagtttttac tattatagca caatttaaag gcaatgaaca gaataaaatt
     3481 cttgaatttt cagaaaattc tatacgcatg tgaaatattt aaaattaaaa acaacaaagc
     3541 ggattagtgc actttctgat gaccagaata tgctttttaa tccgctttct ttaaataacg
     3601 tactgtaatt tttacagaaa ttctttcaga taagtgtatt taacatctat cttgcattat
     3661 aaatttctag aaccttctct tttatattcg attcactcaa accatactca ttaagaagat
     3721 aatccatttt ccctacttga ccgaatcttt cttgaacacc catccgatga atttttgtta
     3781 ttccatcatc agagaataat tcacataaag cactgccaat tccacctatc tgattgtggt
     3841 tttctacagt aaatatagtt tttccactta acattgtttt tatctgttct ggtatcggtt
     3901 tgattctaaa taaatctatc acacttactg aataacctaa tttagacagt tcatctgcaa
     3961 ctcgaatact tggagcaacc attataccag aagcaacgat tacaagatct tcaccatgcc
     4021 ttaactcaat gtagccttta gaaaaatctt ctccaccttg atacacagga actggagctt
     4081 ttctaattgt tcgaatatat tttagtcctt ttaagtctaa tgtctggttc aatatttcac
     4141 gaaattggat atcatcagtt gcttcaaaaa tgatcgattt aggaattaaa cgtaacaatc
     4201 caatttcttc aaatggcata tgtgtcccac cattcatttc tgcagttact cctgcatctg
     4261 atccaatcac agtggcatcc aattgtgcgt atccaagaga aataaataat tgatcaaata
     4321 ctcttcgtga agcaaaagga ccaaatgtat gaagataagg tctaaacccc tgaatagaca
     4381 agcctgctgc aaggccgacc atttctgctt ccataatccc aacattcaca taacggtctc
     4441 caaagtcctt ttcaagatta tgagtagcca tcgaacttga caaatcggct tctaagacta
     4501 ctatatcaga atcactttga ttagcctcta aaaggaagtc tctatataca tgccgtaatt
     4561 ctttcgtgct tctcatcatt ctgtttcctc caattcctga cttaatcttt ctacaactga
     4621 ggttaacatt tgtctctcct ctacagtagg gcgaagatga tgattggatt tcatttcttc
     4681 cagctcttga accccttgac ctttaatagt atctaataca atgcacttag gtgatgaatt
     4741 atttgactgt tttaattgga caatcccttc ataaatttct ctaatatctg aacccttgac
     4801 cctaatggat tcaaatccaa atgctgaaaa tttttctacg aaatcacctg gattacaaat
     4861 atcctttgta aaaccatcta attgtttttt gttatcatca acaaatacaa ttaagttgga
     4921 taactgttga tgagaagcaa actgtatagc ctcccaacat tgtccctcat ttaactcacc
     4981 atctccaaca atagcataag tataaaaggg actctttctt attctctgac catatgcaag
     5041 tccagttgca acactaattc cttgtcctaa agagcccgtt gtcatatcta tgcctggcgt
     5101 tagatttcta tcaggatgag acggtaattt ggttccattt gtatttaaag aatataagaa
     5161 ttctttgtca aagaaaccat tcaaatagag tgtactgtat agagctggtc ctccatgacc
     5221 ttttgataat atgaaataat ctctatctcg tgctgcaaat atttctggag tcattggcat
     5281 tatttcacca taaagcactg ctaaaacttc tacgatagac agacttcctc cgtaatgtcc
     5341 gaatccaaga tgattcaatg ttctaagagt atttaatcgg atgttagtcg caaattttct
     5401 taacccatct tctctatttt tacttaaaat catcccttat tcctcctttg cagatggctt
     5461 tttaataaag gatactccaa acataactgc tagaataaga acaagaccaa tcacaatgcc
     5521 tgcttgtgag ccaaattgat ttaacattcc taaaataatt cctgatagac caaaatctgc
     5581 atctgagaaa gttgatcctt ggaaaccaag tcctcccaaa actggcatta aaaagactgg
     5641 aagaaaactg attaaaatac cttgtaaaaa tgctccaata gtggctccac gaacaccacc
     5701 agatgcattc ccaatgacac ctgcagtcgc tccacagaag aaatgaggca caacacctgg
     5761 taagataaca accgttcctg aagcaatcat aattgccata cttactaaac caccaataaa
     5821 actagagata aatccaatta gaactgcatt gggtgcataa gtataaacaa tcggacaatc
     5881 caaagcaggt tttgaattag gtacaagacg ctctgaaata cctttaaagg ctggaacaat
     5941 ttcgcccaaa ataaggcgaa cacctgctaa aataacaaat acccctgctg caaattgacc
     6001 tgctaattgt aaagcataaa ctagaccact tgtaccacta ctgatttctt tttctatata
     6061 ttctgaccct gcaaagatag ctacaataat gtaaataact gccatggata aagtaatact
     6121 aacagtacta tcacgtaaaa aagctaaact ctttggaaat ttaatgtcct ctgttgattt
     6181 tgatttgtca ccgataaggc taccagtaaa accactcaac caatatccca aagaactgaa
     6241 atgacctaaa gccaccttgt catttccagt taattgaacc atatattttt gaacaaatgc
     6301 tggggaaata ctcataataa taccgagtgc taatcctcct agtaagataa gaggcaagct
     6361 agtaaagcca gcaactgata aaatgaccgc aatcatacat gccatatata gagtgtggtg
     6421 ccctgttaaa aaaatatatt taaatcgagt aaaacgagcg attaagatat tgaacaccat
     6481 gcctgcaaac ataatcagtg cagtagctga gccatatgtt gttaaagcta cagctacaat
     6541 tgcttcatta ttcggcacaa cgccagataa atgaaaagca tgctcaaaca tggtaccaaa
     6601 tggatttaaa gaattttgta caattcctgc accaccagat acaactaaga aaccaacaaa
     6661 ggtcttaatt ccacctttaa taatatcagg taatttcttc ttctgaagaa ctaatcctaa
     6721 aattgcaatt aaagctacta aaatagctgg tgtactaaca atatccaata tgaacttcat
     6781 catgacgcta gcctcctata taagtccttt ttcttcacaa agtttagtaa ttaattctcg
     6841 tagttcatcc atatcaataa tactatttaa gatacgaaca tctccaagat gactagctga
     6901 atcagctaga tcacgaccaa caatccaaat atcagctgca tttggatctg ctccacctaa
     6961 atcataatgt tcaacttcta catctgaaac attcaaatca ctcaatacag attcaatatt
     7021 catctgtacc ataaaacttg aacctaatcc tgaaccacaa gctgtaccaa tttttaacat
     7081 tatctaatcc tcctgtttaa ttatcatttt aatgtcatca tagttttttg atgatattaa
     7141 agtttgaaca tgatttttat ctcttaaaat tgttgttaaa tgtgacaaag cctttaaatg
     7201 actctcatta tcaatggctg caatacaaat caacaatctt acctcttgtt ctggattatc
     7261 caataaataa atcggttctt ccaaaactaa cattgacatt cctatttcat tcacaccttc
     7321 atctggccga gcgtgaggaa ttgctactcc cttccctaaa ttaataaaag gcccaaactc
     7381 ttctactttt tgaatcattg cctcagggta gttctcagtt atcttatctt gatccaaaag
     7441 cggtttagct gctaaacgaa tcgcctcctt ccatcctaat ttttgcgaac taacctgata
     7501 ggtttctttg gtaatgagtt gttctagcac tggtacaatt tcctttctat catttttttg
     7561 gtaaagataa ttctttaacg ccaatcttaa ttccaattct tgtgtaataa ttccatatct
     7621 tttgacaata ttcagaattt gttcaatctc aaaatcccca tactctaaat tcggaaaatc
     7681 ttttaacact agttctacta gttgtattgc ttgctcttca gtcatcataa ccgaaactag
     7741 ataatttggc ttttctgtct ccacctttat ggtagaaaaa accatatcat agtcactact
     7801 agctttcacc tgtaaatcat caatctttga ggttcctata aactcaattt gaggaaataa
     7861 tgctaataga ttctctttta acatcaatga agaactaaca ccattaggac aaatgattgc
     7921 tgctttatac catttttgag gcaaagtatc tgctttcttt aaataacctc cgaaatggat
     7981 aacaaaatat gctgtttcac tatcaggtat gggattgtca atagcgtcca tcaagggcat
     8041 caaagaatct ttgactaatt caaataaatc aggataatat tctttaacat gcaatacata
     8101 ttcatttgaa ctaggtaggc cgaactttaa tctatagtaa gccggtataa ggtggcggcg
     8161 aagattttct ctcaatcctt ccctttgttt aaaatgtaac aaagaaatat cttccattct
     8221 acttataata gcctctgtta attgattaaa gtaaaccgga gcaacatcta cttcaccttc
     8281 aaagcaactt gataataaaa ctgtgatata gcgataatca tcctcagaaa acaccgtatc
     8341 tataattccc aaatcaacca ctgtatccaa taaaatagtg gttatatctt gaataacagg
     8401 agatactaat gtctctgaaa gacatactct ttcaacatcc ctttgatacc tacacagaat
     8461 gaatactaaa ccgaaaaggc aaacttttaa ttgattaaca ataggtacta gttgtagctt
     8521 ctcataataa tctttaacta cctgatttat caaatcataa gttaatgaat acccccaact
     8581 ggataaaaca taatccaaac cccaaatccc tatggaggat tccagcaact cactaaccat
     8641 ttgaaaagct aagcggtgct tattccactc tgaaccgtgt aaagtataac cttttgctct
     8701 actgtaccct agctccaaat cattatctaa cataatcttt cttaatgatt gaatatcaga
     8761 taaggttgta ttcttactta ctttcaaaaa gtcttggtaa tgattattcg atataaaatc
     8821 taatcggcaa aaagtgtaaa gatagattaa agctaagcga gtcgactttg gtaaaaccaa
     8881 ttcatccgac ttaataatat ctgtcaaaga ctgcttcgta cgatttgata aactatagcg
     8941 accttgtttt ttatccagca ctatcccttt attagctaga taaggcacta aataatctat
     9001 tccttctttg acttccttta taggtaagct caccttaaca gataattcat ataatgatag
     9061 ctcacaatga tccatcaaag tcatcaaaat aactagtgct ctataatcaa acatttctct
     9121 tcctttctaa ctacaattat agtgtaaaaa ttataagaat aaaagagtat ttgagcacaa
     9181 tataatctca ccttttaaaa gaaattattg ggctgaaagt ttttagtgat tattttcaac
     9241 tttctattga cttttaaaaa caaagtgata tcatttagat atcaaataat gggaggtcat
     9301 tcttatgact gaaaaactaa tcaattcaaa accaaatggt gtattcgcat tgattctcat
     9361 tgagttgaca atcgtacttg gtgtctttat atttataatg ggtgttggtt cggaaaacat
     9421 ttttggaatt attatcggac ctttactaat cgtaattgca ggtctagctc atgctggttt
     9481 aaaagttgtc aaacctcagg aagctctggt tctgacactc tttggtaact atacaggtac
     9541 catcaaagaa cctggttttt actttgtcaa tcccttcagc gtagcagtca accctgcaaa
     9601 ccacactcga cttggacaaa gtggtgatgt tagcacaaaa tctccttttt taggagctaa
     9661 atcatcaaat gacaatgatg taaatcttga aattggcaag aaacagattt ccctcaaagt
     9721 catgaccttg agcaattctc gtcaaaaaat caatgattgc ttaggaaacc ctgtagaaat
     9781 cggtatcgcg gtaacttgga gagttgtcga taccgctaag gcagtcttca acgttgataa
     9841 ctacaaagaa tatctttcat tgcaatgtga tagcgccctc cgtaatattg tccgcatcta
     9901 tccttacgat gtgtctccta atgtggatac tacgggtgac gggcaagcag atgaaggtag
     9961 tctccgtggc tctagcgaaa ttgttgctaa ccgtattcgt gaagaaatcc aaagtcgtgt
    10021 tgaggatgct ggcttggaaa tccttgaagc acgtatcact tacctagctt atgctccaga
    10081 aattgctgcc gttatgcttc aacgccaaca agcatctgcc attattgatg cacggaagat
    10141 gattgtagat ggtgctgtag gaatggttga aatggcacta gaacggctca atgaagggga
    10201 attggtagaa cttgacgaag aacgaaaagc tgccatggtt tcaaatctcc ttgtcgttct
    10261 ttgtggcaat catgatgcac aaccaattgt caacacagga agtctttact aaagatggct
    10321 gaagctaaaa aaaagcagat cccccttcga ctctcaacaa agttatacgc tgcactcgca
    10381 tcatgggcag aagacgactt tcgttcagtc aatgggcaaa tcgaatatct cctcacagag
    10441 tgtgtcaagc aacggaagaa agacggaaaa tacgtatcag aaaccattga cgaaccattt
    10501 gagattgata tttaacatct tctcctgtct gacaagtcgg acaggagaat tttttcatct
    10561 ttttttgtca agtaacttta ctttacaaaa aaaatgtgtt atcctagtat ggttgatgaa
    10621 aatcagtaga ttgaatcgaa tataaatacc taaaggagaa atcaaaatgg cagtacctgc
    10681 acgtcgcact tcaaaagcga agaaaaacaa acgtcgtaca cactacaaag taacagctcc
    10741 atctgtaaac tttgacgaaa ctactggaga ttactcacgt tctcaccgtg tatcacttaa
    10801 aggatactac aaaggacgta aaatcgctaa agctgcatca gctgaataat agaagggaga
    10861 taccatgcgc gtaaatatta cacttgaaca caaagaatct ggtgaacgct tgtaccttac
    10921 ttctaaaaac aaacgtaaca ctccagaccg ccttcaattg aagaaatact caccaaaact
    10981 tcgcaaacac gttgtgttta cagaagttaa gtaagcattc aatacgaatc aatacagaag
    11041 aaaaacgctt gatcagcgtt ttttcttttt gttaatttca ctatattgca agggaaatca
    11101 ttcgaatgac tacatttttc actacctttt ttgaaataag aatacggatt cggacgatta
    11161 aaagtaagag aaactcaaat gtaagttggg gttccttttt tgtatatttt tcaaataacg
    11221 cctctacact atttgtagca aattcaccaa ctacagttgt atcttagtta aaataagtta
    11281 gaatatgtaa gtgagtacca gatataccaa gacatcgtca ccatctaagg tatattcaaa
    11341 atacaaaagt tgaccaacta gatttctgaa tatccttata tatccattct taaaattggt
    11401 ttaaatagcg tagtctttta aactagtttt gagaatccaa aaaatcttcc tacatatgta
    11461 agaagatttt ttagttcaga atgattagat ttagctaatg gatacctatc ctacccactc
    11521 accgttagca ttgactcgat agccatcggg tgtagtcgtg tttactaata aatctcctga
    11581 attataggta tagtaccatt taccagaaac tgtaa
//
LOCUS       DAVLVF010000054        11591 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVLVF010000054 DAVLVF010000000
VERSION     DAVLVF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.054"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            51..200
                     /gene="blpO"
                     /locus_tag="VYN66_001408"
     CDS             51..200
                     /gene="blpO"
                     /locus_tag="VYN66_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV1495420.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            304..423
                     /locus_tag="VYN66_001409"
     CDS             304..423
                     /locus_tag="VYN66_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV1495421.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            891..1262
                     /locus_tag="VYN66_001410"
     CDS             891..1262
                     /locus_tag="VYN66_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495422.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLTSIL
                     VTVYLTREMRLREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
     gene            1891..2274
                     /locus_tag="VYN66_001411"
     CDS             1891..2274
                     /locus_tag="VYN66_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV1495423.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            2326..3015
                     /locus_tag="VYN66_001412"
     CDS             2326..3015
                     /locus_tag="VYN66_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1495424.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            3057..3290
                     /locus_tag="VYN66_001413"
     CDS             3057..3290
                     /locus_tag="VYN66_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495425.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            3441..4052
                     /locus_tag="VYN66_001414"
     CDS             3441..4052
                     /locus_tag="VYN66_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1495426.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            4213..5007
                     /locus_tag="VYN66_001415"
     CDS             4213..5007
                     /locus_tag="VYN66_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV1495427.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            5004..5639
                     /gene="trmB"
                     /locus_tag="VYN66_001416"
     CDS             5004..5639
                     /gene="trmB"
                     /locus_tag="VYN66_001416"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV1495428.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            5765..6244
                     /gene="rimP"
                     /locus_tag="VYN66_001417"
     CDS             5765..6244
                     /gene="rimP"
                     /locus_tag="VYN66_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV1495429.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            6288..7424
                     /gene="nusA"
                     /locus_tag="VYN66_001418"
     CDS             6288..7424
                     /gene="nusA"
                     /locus_tag="VYN66_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV1495430.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            7446..7739
                     /locus_tag="VYN66_001419"
     CDS             7446..7739
                     /locus_tag="VYN66_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV1495431.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            7732..8031
                     /locus_tag="VYN66_001420"
     CDS             7732..8031
                     /locus_tag="VYN66_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV1495432.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            8048..10840
                     /gene="infB"
                     /locus_tag="VYN66_001421"
     CDS             8048..10840
                     /gene="infB"
                     /locus_tag="VYN66_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV1495433.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            11091..11441
                     /gene="rbfA"
                     /locus_tag="VYN66_001422"
     CDS             11091..11441
                     /gene="rbfA"
                     /locus_tag="VYN66_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV1495434.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3539 a         2035 c         2593 g         3424 t
ORIGIN      
        1 tgataatttt tatattaaac ttgttccgta actgaaaagt gacatactat atgaatacaa
       61 aaatgatgtc acaattttct gttatggata atgaaatgct tgcttgcgtt gaaggtggag
      121 atattgattg gggaagaaaa attagttgtg cagcaggggt tgcatatggc gcaattgatg
      181 ggtgtgcaac aacggtttga catttctatt gggaccattt gctataggaa taggtgtaac
      241 tggtgctgca ggtggagcta tacttggagg tgtagcctat gcagcaacat gttggtggta
      301 attatggatt ttaaaagttt tattattggt ttagtagttg gtatatttgg tccttatatg
      361 gatgacttaa ttagaaaaat attttcaaaa tcttccaaaa aggatacgga tagtactctc
      421 taaaaaataa atttctcccc tgtatttgct agcttaattt acaggggagt ttctttaata
      481 ttgtttcgcc tgtcaaaatg atatgtgata gcaggatgaa aagtctacaa acaagttggt
      541 atcaatacaa tataaagagg aaagtatgtt tttcatgctt gcttttttaa tttttacaat
      601 tcaagaggtt ttgatgacca tttatgattt gagtgatcca aggtcaaaat aagtgctata
      661 ctagcagtgt aaatgttctt gctcaactaa gataaatcgc acatttagga ggaaaactta
      721 tgaagaaaaa aatactgatt attttcgttc tgtatctgat catgtccatc tttctttatc
      781 cgcttaggga gagtatttgg tataatctat tttataccat agcctatatg attgcggtta
      841 tgatctattt ttctttaatt aaaaagaaag aaaagaaatg aaagattttc ttgattaaaa
      901 agaatcatat tctttgtgag attgttcctg ggtcagttgc ctttgcttgt ttctacttat
      961 ctatttctat ttcgtcagtt ttccaatttc ttttagtagt tattaacttg acttctattt
     1021 tggttactgt ttatcttact agggaaatga gattaagaga gtttgaagat gatgatttgg
     1081 ttagtcctag aaccaatcaa ctcatgttta tcggcttgac aggctttatg tctattattt
     1141 gtttgtatag aggtatcaca gcaggagaat cttatcaaca actaatcgct tatattggtg
     1201 ctattctttg cttgatcatc atgcttctac tcatttgggg tttgaagtat tataaaaagt
     1261 aggggctaat gaagtttttg caatttagga ggtttcgatg acaattcaag atttggatga
     1321 aaaattttaa aaaacaatga tatactaaac ttgtcaaagt tgcaacgggt gctgctacat
     1381 tatgttaaaa atcgctcttg attgataagt tggatttgtt aggaaaggag taaataatta
     1441 tggataagaa aaagatagtt tcgactataa tatgtatagt atttcttgta gtgtcagtag
     1501 ataatttttt tagagactta accccgttac tgttcatttt aaatattatt ggcctgtctt
     1561 gcttttcagt gctaacatac attaacataa aagagatact cttaaatata agtaaatgat
     1621 gatttggtta gtcctagaac caatcaactt atgtttatcg gcttggcaga ttttatgttt
     1681 gtcatttgtt tgtatagagg tatttcagaa acagaattct atcaacaact aatcgcttat
     1741 ataggtgtat tctctgcttg tttatcacgc ttctgctcat gtggggcttg aagtattata
     1801 aaaagtagct ttctatcctt ttctgacagg atatttgagt agaaggctct attaaattgt
     1861 tagtgaaata aatgaatgga ggtatttaat atgaaataca gattattttt tgttattttc
     1921 ttgagtagtg tgttggatat tcttttaggg acatttttac aaatctctat cgtatctatt
     1981 gggtggcttg ttctttacag tggtttgttt gaagcaggag ttttccttct tgctaataaa
     2041 ggggtggcgg taaaaatcaa ggaagtagat attcgaaatc gctttaaatt tatttttgga
     2101 aaaaccttat ggtttcaaat tcttttgctc atctttttga taatcaaact ttatcttggt
     2161 ttggatgcga ggttgatttt attctatgga catattttca ttgtctttaa tgccttaatg
     2221 tatctattat ctagtagtca ggttagcctt aaaaaaaaca aactgtcttc ttaatcttac
     2281 agcagtagag cctgacaagg tgaggtcata gtagggagga tttctatgaa aaagtatcaa
     2341 cttctattca aaataagtgc agttttctct tacttatttt tcgtatttgg tctttctcag
     2401 atgacgctta ttattcaaaa ttattggcaa ttttcttccc agattggcaa tttcgtctgg
     2461 attcaaaatt tcttgagttt gctatttagc ggagtcatga tttggattct ggttaagaca
     2521 ggtcatggtt atctctttca cattccaaga aaaaaatggc tttggtattc gattttgaca
     2581 gtattagtgg tagtgctcca gatctctttt aacgttcaga cagctaaaca tgttcagtca
     2641 actgctgaag gttgggctgt attgatcggt tatagtggga ccaactttgc tgagctaggt
     2701 atctatataa ctttgttctt tctgactcca cttatggaag agctaatcta tagaggatta
     2761 ctgcaacacg ccttttttaa gcattcgaga tttggccttg atttgcttct tccgtcaatt
     2821 ttatttgctc ttcctcattt ttcaagcctg cctagtttgt tagatatctt catctttgca
     2881 acatctggca tcatctttgc tagtttgacc cgctatacca agagcattta tccttcctat
     2941 gcggtgcatg tgatcaataa tattttcgca acattaccat ttttgctgac ttttttacat
     3001 agggtcttgg ggtaaaaaaa caaaaggctt gcttttcagc catagaggag attatcatgt
     3061 ataaacactt atttttccta gattccaaaa ctttagatcg gttgacaccc tatattctag
     3121 tcttggcttc tgacaccatt gcttttaatg tttttgtgct aacctttgta tctgcggtgg
     3181 tttttaattt cctaaattcc atgctagctt taatggctat attcataggg gctggctatg
     3241 tggtcggatt ttggttacta atactcaatg aaaatcaaag agcaaactag gaaactagcc
     3301 gcaggctgta cttgagtacg gcaaggcgac gttgacgtgg tttgaatttg attttcgaag
     3361 agtataaaat ggtttgtttt ggaaaggcta gaactaaaga atgacgcgta ggaagtctgt
     3421 ttggtgagga ggatagtttt atggagtttt ttgataaatt tcatgccttg tgttttggat
     3481 ttttagtact aataattgtc attacagttc cttatacgat taaccatggg ggtttttttc
     3541 aaaatgaatc tgcattgatt cttgtaagtc ttcttgtaac ctcgctgagt gttgcttatg
     3601 ctagaaagtt tgaaatgatt tcttttggga tgttaagcaa gaaacaactt ttgcttttca
     3661 ttgcaatctt tcttctaagt gtacttgaga cgctggttta tattcatttc ttcgctgttt
     3721 cttctggctc aggggtccaa cacttggcgg aagtcagcag aggaatttcc ctgtctttga
     3781 ttttgactac ctcagttttt ggccccatcc aggaggaact cattttcaga ggacttcttc
     3841 aaggtgcggt ttttgacaat tcttggttag ggcttgtgct aacttcctct ctcttttctt
     3901 tcatgcatgg accttctaat gtcccttcgt ttatttttta tctacttggg ggtttgttgc
     3961 tgggctttgc ttataaaaag agtcaaaacc tatgggtttc tactctagtc cacatgtttt
     4021 acaacagttg gccactccta tattatttat aaaaattatg aaaaggtaag cagaagatgg
     4081 tgcttacctt tttcaagcta taatgatacc caagccaatc cagaggcttt tctttgagaa
     4141 tcgggttaga gtggttgacg aataggccaa aaactagtag aatagtaagg aaactttata
     4201 cggaggaaag aaatggattt gggtgataat gagctaacac tgactcccat acctgggaaa
     4261 agtggcaagg cttatatggg tagctatcct gacgggaagc gcatctttgt aaaaatgaac
     4321 acctctccaa tcctacctgg tctagctaga gaacaaattg ctccacaatt attatggagt
     4381 cgtcgtttgg cagatgggcg tgatatgtgt gctcaagaat ggttgacagg caagatattg
     4441 accccctatg atatgaatcg taagcaaatc gtcaatattt taacccgtct tcatcgctca
     4501 cgtccgttga tgacacaatt gagtcgtttg ggctatgcca tggaaacacc tgtagattta
     4561 ctacagtctt ggcaggaaac ggctccagat gctttgcgta aaaatcattt tatcagtgaa
     4621 gtgatggctg atttacgtca gactattcca ggatttagag aggaccatgc gaccattgtc
     4681 catggagatg tacgacatag taattggatt gagacagata gtggcttgat ttatttagta
     4741 gattgggatt cggttcgctt gaccgatcgc atgtttgatg tggcccatat gctctgccat
     4801 tatatttcag aacatcagtg gaaggaatgg ttgacctact acggttacaa gtacaatcaa
     4861 acggtattaa gtaaattgta ttggtatggt caattgtctt atttgagtca gatttccaag
     4921 tattatatga accaagattt agaaaatgtc aatcgggaga ttcatggttt gcgtcatttc
     4981 cgagacaagt atggaaagag aagatgagag ttagaaatcg taaaggggca acagaattac
     5041 tagaggcaaa tccccagtat gtggtcctca atcccttgga agccaaggca aaatggcggg
     5101 acttgtttgg caatgataat cccattcatg tggaagttgg aagtggaaag ggtgcctttg
     5161 tttcaggtat ggccaagcaa aaccctgaca tcaactatat cgggattgat attcaaaagt
     5221 ctgttttgag ctacgctttg gacaaggtgc ttgaagttgg agtgcctaac atcaagctct
     5281 tgtgggtaga tggttctgac ttaactgact attttgaaga cggtgagatt gatcgcttgt
     5341 atctgaactt ttcagatcca tggccgaaaa aacgccatga aaagcgtcgt ttgacctaca
     5401 agaccttctt ggataccttc aaacgtatct tgcctgaaaa tggagaaatt catttcaaga
     5461 cggataaccg tggcttgttt gagtacagtt tagtgagctt ttctcaatat ggcatgaaac
     5521 tcaatggtgt ctggttagat ttgcatgcca gtgattttga aggcaatgtc atgacagaat
     5581 acgagcaaaa attctcaaac aaggggcaag ttatctaccg agttgaggca gaattttaag
     5641 agatagccta aaattaggct gtacaagtgc ttttgcttta cataagttgg caaacgtgct
     5701 atactgatag taagaatatg aaaagtgagg cggggaaata tcttcgcctc ttgcttatga
     5761 ggaggtggac gcaatcgcaa caatcgtaga attagtcaga gaagttgtag aacctgtcat
     5821 agaagctcct tttgaactcg tggatatcga gtatggaaag attggcagtg acatgattct
     5881 cagtattttt gtagataaac ccgaaggaat taccttgaac gacacggcag acttgacaga
     5941 aattatcagt cctgtcctag acaccatcaa gccagatccc ttcccagaac aatatttcct
     6001 agaaattacc agtccaggtt tggaacgccc tttgaaaacc aaggatgccg tcgctggagc
     6061 ggttggaaaa tacatccatg tcgggctcta ccaagccatc gataagcaaa aggtctttga
     6121 aggaaccttg ttggccttcg aagaggacga gttgactatg gaatatatgg acaagacgcg
     6181 taagaaaacc gtccaaattc catacagttt agtatcaaaa gcacgtttag cagttaaatt
     6241 atagaaaaag aaaggatagc ttttgaggat tcaaaagtga agaaaacatg agtaaagaaa
     6301 tgctagaggc cttccgcatt ttggaagaag acaagggaat caaaaaagaa gatatcatcg
     6361 acgcagtagt agagtcgctt cgttccgctt atcgcagacg ctatggtcag tcagacagcg
     6421 tagctattga cttcaacgaa aaaacaggtg actttacagt ttatactgtc cgtgaagttg
     6481 ttgatgaagt atttgatagc cgtttggaaa tcagcttgaa agatgctctt gccattaatt
     6541 cagcttatga acttggagac aaaatcaagt ttgaagaagc accagctgag tttggtcgtg
     6601 tagcagccca gtctgccaaa caaaccatca tggaaaaaat gcgcaagcaa acacgtgcca
     6661 tcacttacaa tacttacaaa gaacatgagc aagaaatcat gtctggtaca gtagaacgct
     6721 ttgacaaccg ctttatctat gtcaaccttg gtagcatcga agcccaattg tcaaaacaag
     6781 accaaattcc tggagaagtt tttgcttctc atgatcgtat cgaagtttat gtttacaagg
     6841 ttgaagacaa ccctcgtggt gtgaacgtct ttgttagccg tagtcatcca gaaatgatca
     6901 aacgtttaat ggagcaagaa attccagaag tttatgatgg aactgttgaa atcatgagcg
     6961 tggctcgtga agcaggtgac cgtacgaagg ttgctgttcg tagccacaat ccaaacgtgg
     7021 atgctatcgg tacaatcgtt ggacgtggtg gtgctaatat caagaagatt actagcaaat
     7081 tccacccagc tcgttacgat gctaaaaatg accgcatggt accaatcgaa gaaaatatcg
     7141 atgttatcga gtgggtagca gatccagctg aatttatcta caatgccatc gctcctgctg
     7201 aggttgacca agttatcttt gatgaaaacg acagcaaacg tgccttggtg gttgttccag
     7261 ataacaagct ttctcttgcc attggtcgtc gtggacaaaa cgtgcgcttg gcggctcact
     7321 tgactggtta ccgtatcgat atcaagtctg ctagcgaatt tgaagccatg gaagacgctg
     7381 cttcagtaga gttggaagta gaaaacgata ctgtagaaga ataaaagctg ctagaggagg
     7441 gaaagatgaa aacaagaaaa atccctttgc gcaagtctgt tgtgtctaac gaagtgattg
     7501 ataagcgtga tttgctccgc attgtcaaga acaaggaagg acaagtcttt attgatccta
     7561 cgggcaaggc caatggccgc ggcgcttata tcaaactaga caatgcagaa gccctagagg
     7621 cgaaaaagaa gaaggtcttt aaccgcagct ttagcatgga agtggaagaa agcttttatg
     7681 acgagttgat cgcttatgtg gatcacaaag tgaaaagaag agagttggga cttgaataag
     7741 caaaagataa gtaatctctt ggggcttgct cagcgagcag ggcgcatcat atcgggtgaa
     7801 gaattggtgg tcaaggccat tcaagacggc aaggccaagt tggtctttct agctcatgat
     7861 gctggaccca atctgaccaa gaagattcaa gataaaagtc attattatca agtagaaatt
     7921 gtaaccgtgt tttcaacact ggaattaagc atagcagtcg ggaaatcgag aaaggttttg
     7981 gctgtaacag atgctggatt tacaaagaaa atgaggtctc ttatggaata gaagaggagg
     8041 acatgatttg tctaagaaaa gattgtacga aatcgcaaaa gaacttggaa aagaaagtaa
     8101 agaagttgta gcgcgtgcaa aagagttggg cttggatgtg aaaagccact catcaagtgt
     8161 ggaagaagct gtcgctgcaa aaattgctgc cagctttaag cctgcagctg ctccgaaagt
     8221 agaagcaaaa cctgcagcac caaaagtaag tgcagaaaag aaagccgaaa aatctgagcc
     8281 agctaaacca gctgtagcta aggaagaggc aaaaccggct gagccagttg ctccgaaaac
     8341 agaaaaagta gcagtgaaac cgcaaagtcg taatttcaag gctgagcgtg aagcccgtgc
     8401 taaagagcag gcagaacgac gcaagcaaaa taagggcaat aaccgtgacc aacaacaaaa
     8461 cggaaaccgt cagaaaaacg acggccgtaa tggtggaaaa caaggtcaaa gcaaccgcga
     8521 caatcgtcgc tttaatgacc aagctaagaa gcagcaaggt cagcaaaaac gtagaaatga
     8581 gcgccgtcag caagaggata aacgttcaaa tcaagcggct ccacgtattg actttaaagc
     8641 ccgtgcagca gccctaaaag cagagcaaaa tgcagagtac gctcgttcaa gtgaggaacg
     8701 cttcaagcag tatcaggctg ctaaagaagc cttggctcaa gctaacaaac gcaaggaacc
     8761 agaggaaatc tttgaagaag cggctaagtt agctgaacaa gcacagcaag ttcaagcagt
     8821 ggttgaagtc gtccctgaga aaaaagaacc tgcagtggat acacgtcgta aaaaacaagc
     8881 tcgaccagac aaaaatcgtg acgattatga tcatgaagaa gatggtccta gaaaacaaca
     8941 aaagaatcga agtagtcaaa atcaagtgag aaatcaaaag aatagtaact ggaataacaa
     9001 caaaaagaac aaaaaaggca ataacaagaa caaccgtaat cagactccaa aacctgttac
     9061 ggagcgtaaa ttccatgaat tgccaacaga atttgaatat acagatggta tgaccgttgc
     9121 ggaaatcgca aaacgtatca aacgtgaacc agctgaaatt gttaagaaac ttttcatgat
     9181 gggtgtcatg gccacacaaa accaatcctt ggatggggaa acaattgaac tcctcatggt
     9241 ggattacggt atcgaagcca aacaaaaggt tgaagtggat aatgctgaca tcgaacgttt
     9301 ctttgtcgaa gatggttatc tcaatgaaga tgaattggtt gagcgtccac cagttgttac
     9361 tatcatggga cacgttgacc acggtaaaac aacccttttg gatactcttc gtaactcacg
     9421 tgttgcgaca ggtgaagcag gtggtattac tcagcatatc ggtgcctacc aaatcgtgga
     9481 aaatggtaag aagattacct tccttgatac accaggacac gcggccttta catcaatgcg
     9541 tgcgcgtggt gcttctgtta ccgatattac gatcttggtc gtagcggcag atgacggggt
     9601 tatgcctcag actattgaag ccatcaacca ctcaaaagca gctaacgttc caatcatcgt
     9661 agctattaac aagattgata aaccaggtgc taacccagaa cgcgttatcg gtgaattggc
     9721 agagcatggt gtgatgtcaa ccgcttgggg tggagattct gaatttgttg aaatctcagc
     9781 taaattcaac caaaatatcg aagaattgtt ggaaacagtc cttcttgtag ctgaaatcca
     9841 agaactcaag gcagacccaa cagtgcgtgc aatcggtacg gttatcgaag cgcgcttgga
     9901 taaaggaaaa ggtgcggtcg caacccttct tgtacaacaa ggtaccttga atgttcaaga
     9961 tccaatcgtt gtcggaaata ccttcggtcg tgtccgtgct atgaccaacg accttggtcg
    10021 tcgtgttaaa gttgctggac catcaacacc agtctctatc acaggtttga acgaagcacc
    10081 gatggcgggt gaccactttg ccgtttacga ggatgaaaaa tctgcgcgtg cagcaggtga
    10141 agagcgtgcc aaacgtgccc tcatgaaaca acgtcaagct acccaacgtg ttagccttga
    10201 aaacctcttt gataccctta aagctgggga actcaaatct gttaatgtta tcatcaaggc
    10261 tgatgtacaa ggttctgttg aagccctttc tgcctcactt caaaagattg acgtggaagg
    10321 tgtcaaagtg actatcgtcc actcagcggt cggtgctatc aacgaatcag acgtgaccct
    10381 tgccgaagct tcaaatgcct ttatcgttgg tttcaacgta cgccctacac cacaagctcg
    10441 tcaacaagca gaagctgacg atgtggaaat ccgtcttcac agcattatct acaaggttat
    10501 cgaagagatg gaagaagcta tgaaagggat gcttgatcca gaatttgaag aaaaagttat
    10561 tggtgaagcg gttatccgtg aaaccttcaa ggtgtctaaa gtgggaacta tcggtggatt
    10621 tatggttatc aacggtaagg ttgcccgtga ctctaaagtc cgtgttatcc gtgatggtgt
    10681 cgttatctat gatggcgaac tcgcaagctt gaaacactac aaagacgacg tgaaagaagt
    10741 gacaaacggt cgtgaaggtg gattgatgat cgacggctac aatgatatta agatggatga
    10801 tgtgattgag gcgtatgtca tggaagaaat caagagataa gattttttgc tcctttctta
    10861 ggtggtgagg gacgcaagca aaccgatggt ttcattgctt atttttgagc ctagggtctc
    10921 aaaaatcccc tgtgatggga ctgataaatc agttccatca ctttcaccac ggcgaaagaa
    10981 gcagatgact tcaaattgaa cttcgtttca atttaaactg aaaatcaaga agtttaaaat
    11041 agctaggtct gctggcctag cttttggttc aaagtagaga aaggaatatc atggcaaatc
    11101 atttccgtac agatcgtgtg ggcatggaaa tcaagcgtga agtcaatgag attttgcaaa
    11161 agaaagtccg tgatccacgt gtccaaggtg tgaccatcac agatgttcag atgctgggtg
    11221 acttgtctgt tgccaaggtt tattacacca ttttgagtaa ccttgcttcg gataaccaaa
    11281 aagcccaaat cgggcttgaa aaagcaactg gtaccatcaa acgtgaactt ggtcgcaatt
    11341 tgaaattgta caaaatccca gatttgacct tcgtcaaaga cgagtccatc gagtatggaa
    11401 acaagattga cgagatgcta cgcaatctgg ataagaacta aagaagaggg gttgcccctc
    11461 ttttttggtg gaggaaaata ggttgaattt gaaatggaaa aatattcttt taaaatatat
    11521 tgaaactaga atagtacgcc tctacttcta aaacattgtt agaaatcgat ttgactgtcc
    11581 tgatcgattt g
//
LOCUS       DAVLVF010000055        11506 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVLVF010000055 DAVLVF010000000
VERSION     DAVLVF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11506
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.055"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(56..286)
                     /locus_tag="VYN66_001423"
     CDS             complement(56..286)
                     /locus_tag="VYN66_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV1495435.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(454..1098)
                     /gene="pcp"
                     /locus_tag="VYN66_001424"
     CDS             complement(454..1098)
                     /gene="pcp"
                     /locus_tag="VYN66_001424"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1495436.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(1113..2036)
                     /locus_tag="VYN66_001425"
     CDS             complement(1113..2036)
                     /locus_tag="VYN66_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV1495437.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(2033..2722)
                     /locus_tag="VYN66_001426"
     CDS             complement(2033..2722)
                     /locus_tag="VYN66_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV1495438.1"
                     /translation="MEWIRLIGIAIIVVGFIFKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(2787..3491)
                     /locus_tag="VYN66_001427"
     CDS             complement(2787..3491)
                     /locus_tag="VYN66_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1495439.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(3547..4569)
                     /locus_tag="VYN66_001428"
     CDS             complement(3547..4569)
                     /locus_tag="VYN66_001428"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV1495440.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(4701..7172)
                     /gene="parC"
                     /locus_tag="VYN66_001429"
     CDS             complement(4701..7172)
                     /gene="parC"
                     /locus_tag="VYN66_001429"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV1495441.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(7592..9535)
                     /gene="parE"
                     /locus_tag="VYN66_001430"
     CDS             complement(7592..9535)
                     /gene="parE"
                     /locus_tag="VYN66_001430"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV1495442.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGFKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAVVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            9671..10312
                     /gene="plsY"
                     /locus_tag="VYN66_001431"
     CDS             9671..10312
                     /gene="plsY"
                     /locus_tag="VYN66_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV1495443.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(10355..11506)
                     /locus_tag="VYN66_001432"
                     /pseudo
     CDS             complement(10355..11506)
                     /locus_tag="VYN66_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3270 a         2572 c         2031 g         3633 t
ORIGIN      
        1 ctctcctaca atcaacatgg actattatat catgaaaatt tcaaaaaaac aagacttatt
       61 ttgctaaatt ataattttca atcaaaattt ccacagcttt ttccaatttt ttataaaaac
      121 tatcgacatt cccattcttt ataatggcaa attgtccgtc taattcggca atttctccga
      181 taactttttt accgatagtc aatgtataac cttcaaaact gtctttacca acattaactt
      241 tggcatccgc tacttgaatt tcgatttttt tatctttctt gctcatttca tacctctctt
      301 cactttttct attttacaag aaaatcctaa aactagcaag aaaaaactct atatcaggct
      361 tctctgatat agagttttat gtaaacaacc tcaatcgatt tagcatagct aatagttagt
      421 aaacaagcat aattgactat cctttttagg ttatcagtga gtctcaccgc ctactaactg
      481 caaatcctta tctttgtagt ctacaatagc ttcaatagct acctctatgc ctcttacaat
      541 atcacataga ctcattcctg cagtattcgg cttatttact acttgttcca tcatatatgg
      601 aatatgcatg aaacctgctc tcatattagg gaatttttta tccactaaat aaagagcttg
      661 gtacatcaaa tgattgcaaa caaaggttcc tgcactattg gatacaactg ccggaagtcc
      721 ctgttttttg atagcttgta ccatcgcttt gataggtaaa ctactaaaat aggccgatgc
      781 tccatcaata cgaatcggtg tatcaattgg ttgattgcct tcgttatcag gtatgcgagc
      841 atcatcttga ttaatagcca ctcgttcagg tgttaagccg gtcctgccgc ctgcttgtcc
      901 aatacaaagt acagcatctg gttgatatcg taatatttct gcctctaaaa cttctgacga
      961 cttataaaaa accgttggaa tttctaccca gcgaacttca gccccattaa tctcagatgg
     1021 taataatttt acagcctcca aagctggatt aatcttttca cctccaaaag gattaaaacc
     1081 tgtaaccaat attttcattt tattttcctt tactaaaatg cgagaaagta cattaagaat
     1141 atgtgaataa caatcattac tagagcaaca cctgcttgag cctttataac gccattctga
     1201 tctttcatat ccatcaatgc tgctggtaga gcgttaaaat tagcagccat tggggtcaat
     1261 aaggtcccac aataacctgc tgtcatggca agagcaccag ccacaattgg attagctccc
     1321 agagcaaata caaagggaac tccaacacct gctgtaataa cggtgaatgc tgcaaaagca
     1381 tttcccataa tcattgtgaa tagaaccatt ccaagaacat aggccaaaac tcctataaag
     1441 cgactatctg aaggaacaat accgctaatc agatgagaga taacatcacc aacacctgct
     1501 acagtaaaaa tagcccccaa agcccctaat aattgaggaa caataccact tgttgaaact
     1561 tgctgagtca ttcgattatt ttctgataac agactcttag ggtgactatt ggtaatcaca
     1621 agaacagaaa ttgtagcaaa caaggcggca aggctaatcg aaatcttgct aaattctgga
     1681 atcatttgcg ctaagaccaa cgcaagtatt gccatcagca taactggaat aaaaatttta
     1741 tttttcaacc tgttagattc aatattggct ttcatttcat ctaaggatgg caaggttccg
     1801 atacggactt gcttaaacaa tgttaacagc gataatagga ttacaataat accaatactc
     1861 atatttggca tataggaacc acctataaac gtaatagaca atagagtcca aaatgcagat
     1921 gtcccaagtc gaactgggtt tgttttatct ttataactac aataggctgt atggagaaat
     1981 tgacaaccaa tcacaatata ggtcaactct aatagttgct ttgccaactc tgtcattttt
     2041 gttctcctcc cctagtcttt tttgatatca attttttatc aaataaataa ttgtaaatcc
     2101 ccactacaat aagtgttata acagcaacaa taatagatgt agaagcaatc cctgcataat
     2161 tgctttcata gcctaactga tctaatgttc cccctatcaa gaggactccc ccagcaccta
     2221 caaacgtatt ttgagcaaag aaatttccaa aattttcatt cgcagccgca cgcgctttta
     2281 ttgtctcatc ttcaacctct gttaactttc tacctaattg agactctgca gctgcttctc
     2341 ccataggttg aaccaaaggt ctgacaaact gagggtgtcc tcctagacga attgaaaaga
     2401 aaccagctaa ctctcgaata aaaaaataaa ctgtatagaa gtttccaact gtcagacctt
     2461 taatctttcg aatcaaatcg attgatcgtt gcttgagtcc aaaggtttct gacagcccca
     2521 caagaggcaa ggtaaccata aaaatcgtga gcactcgctg attgctaaat tcttttccca
     2581 aaatctccaa aaattcaacg agagaaacac ctgaaactaa agctgtaacc aaaccagcta
     2641 agactactgt tgcaattgta tcaaatttaa aaataaaacc cacaacaatg attgctattc
     2701 ctattaatct aatccactcc atatcaaact cctttatatt caaaatgaca gtattttaaa
     2761 aattttatca agatcaatac cattccttat ttaatgtatt tttctagttc tttttggtat
     2821 ttgctattgg attccaattt ttctttttgc cattttttaa aaacctcgtt atattctttt
     2881 gttgtaacaa tatctttttg caatttcatt cctttaaaga tatatggttc ccccttaata
     2941 ccaacttgtg agtatggttt tgagaatggt actacgttac ttacaactgg agaaccacca
     3001 gatgaagctg ttggcatcaa taatgaacta tctgtcgacc aagcttgagc tttggcatat
     3061 ttttcatatc tcttctctag gtcagtggtc tcagaaacag catcttctaa caatttctta
     3121 tatttatcca aaccaggttt agctacaaca tccttatctt ttcctttcgt aataccaagg
     3181 tgtttcatgg cagaaccaga ttttggatcc ataatattca agtaagacgc tggatcttga
     3241 tagcttggag cccatcctgt actattcaaa tcatagtctt tttgagaagg agcaacattg
     3301 ccgtatttat cattttccat caaaccatca ataacatttc caataacgtc tgtcctcgat
     3361 gttcgagtcg ctatactgta gcccaatgat gctggatcta ctgcatagac ataagaaaat
     3421 gttgtcggtg catctgcttc tttatcagtt tttccacaag ccactaaaat agctgacgtg
     3481 ctcaggacca ctcctgctgt taagagccac tttttctatt tcataaaaaa tctcctttgg
     3541 tttattttaa tctactttta caatccaacc ttctggcgct tcaatatcgc caaactgaat
     3601 acccgtcaat tcattatata atttacgcgt cacaggacct acttctgttt cactatagaa
     3661 tacatggaaa tcatcaccat gttgaatacc tccaattgga gaaataaccg ctgctgtacc
     3721 acaggcacct gcctctacaa aacggtcaag attatcaatt ggaacatcac cctcaatagg
     3781 agttaatccc aagcgatgtt ctgccaaata aagcaaggaa tacttggtaa tagatggcaa
     3841 gatagatgga ctcaatggtg ttacaaattc attatcagct gtaattccaa agaagttagc
     3901 tgatccgact tcttcaatct ttgtatgagt tgatgggtcc agatagataa catctgagaa
     3961 atgacgtgac ttggccattt ttcctggtaa gagacttgca gcatagtttc caccaacctt
     4021 agccgcacct gtaccatttg gtgctgcacg gtcgtactca tcctgaatca agaagttggt
     4081 tgggaccaaa ccacctttaa agtaatttcc aactggcata gcaaagatgg tgaaaatgta
     4141 ctcttctgcc ggttttaccc cgataatatc tccgacacca atcaaaagag ggcgaagata
     4201 taaggttcca cctgttccgt atggtggtac gtattcttca ttcgcacgga caactgcttt
     4261 acaagcttct acaaacatgt ctgtcggaac ttgtggcatc aagagacggt cacatgtacg
     4321 ttgcagacgt ttagcatttt catcaggacg gaacagttga acactgccat ccttagtacg
     4381 ataagctttc aaaccttcaa atgcttgttg tccatagtga agacttggag aagactctga
     4441 aatatgcaaa gttgcatcct ctgtaagctc tccttgatcc cattgtccat ttttgaaatg
     4501 agcaagatag cgataaggta atttcatata ggaaaaaccg aggttttccc aatcaatcgt
     4561 tactgtcata ttccactcct tattctaaaa acctatttct tatattctac actatttttc
     4621 taaaatagca agtatatttt gtaattttca gaaaatttct ccaataaaaa ccagctctta
     4681 gaactgattc ttcatttcac ttatttatct tcagtaacta cttcctgaag ataagcgtca
     4741 aaaacttctt catctgaaat cgtgtcagaa atgaagcttc cattgctagt gcgttctgac
     4801 aagttcaagt cttgcaatcg gctttcatag attgttcctt tattggattg gacaagcaga
     4861 gtttggtcgt tcacatccac ttccgtactg aagaaatcgc caacaaatcc ttgctctgca
     4921 actgctcctg ccaagaagac acgatgcggt ttgtttttca actcacgcaa gacttgtaat
     4981 cctcgtttgg cacggctggt tgctagaatt tcctcaatgg aaacacgttt caagcttcca
     5041 cgctgggtca agaggtagaa ggacgaagta ttacagataa agccagattg gaggacatca
     5101 tcttctttca aattcatagc cttgacacct gctgccttag caccgacaac cggaacctct
     5161 tcgatattga aacgcagggc ataaccattt tgactaacca agacaacatc atctagttta
     5221 atcggagcca ctgctacaat ctgatctgta tcgtctttga gcttagcata cttgacagac
     5281 ttagatctat aggtccgcca tggagtgaat tcttttcgct ctacccgttt gatttgacca
     5341 aggcgagtca ctgcaaagta ggttgtcgca tcgtcaaact gatccagtac ttccacataa
     5401 aggatttctt cattcgtttc aaagtttgtg atggtttggc tcagatgctc tccgatgtcc
     5461 ttccaacgaa tatctgccaa ctcatggatt ggtctgtaga tgacatttcc aagacttgtg
     5521 aacatcaaga ggtgctgggt tgtcttggca gattgaacaa aaatcaaacg gtcatcatca
     5581 cgcttgccaa tttcttccaa ggtggaagcc gcaaaggaac gtggactggt acgcttgatg
     5641 taacctgcct tggtcacgct gacgtaggta tcttcctcag cgataagact agctgtatca
     5701 atctcaattg ctttcgcagt gtcttctaaa gaactcaaac gaggagttgc aaatttcttc
     5761 ttgacctcac gaagttcttt cttcatgaga ttgtacatag tcctttcatc accgataata
     5821 gccgccagca tagcaatctt ctcacgaagc tctgcttctt cttcctgcaa gacaaccaca
     5881 tcggtattgg tcaaacggta cagttgcaaa gttacgatag cctcagcctg ttcttccgta
     5941 aaatcatagc taactttgag gttttccttg gcgtccgcct tattctcaga agcacggata
     6001 agagcaatga cttcatccaa aatcgaaatc acacgaatca aaccttcgac gatatggaga
     6061 cgtttctcag ccttttcttt gtcaaagcgt gaacgcgcca aaatcacttc tcgacggtga
     6121 gcgatgtagc tagacaggat tggaacaatc ccaacctgac gaggtgtgaa attgtcaatc
     6181 gccaccatat taaagttgta gttgatttgt aggtcggtgt acttaaataa gtagttgaga
     6241 acaagctcag tattagcgtc tttcttaagt tcgatagcga tacgaagacc atcacggtca
     6301 gactcatcac gaacctcagc aatcccagct accttgttat taacacgaac atcatcgatt
     6361 ttcttgacta gattggcctt attgatttca taaggaatct cagtaataac gatttgttcc
     6421 ttaccacctt ttagcttttc aatttcagtc ttggaacgaa caaccacgcg ccctttccca
     6481 gtttcataag ctttcttgat ttcatcacga ccctgaataa tagcccctgt agggaagtct
     6541 ggtccaggca agaattccat gagtttatca atctttgcag ttgggtggtc aatcatgtaa
     6601 actgcagcat ctatgacctc agctaaatta tggggaggaa tgtctgtggc ataaccagcc
     6661 gaaatcccag tcgaaccatt gaccaagagg tttggaaagg ctgctggcaa gaccgttggt
     6721 tctttctccg tatcgtcaaa gttccatgca aaaggaactg tatttttctc gatatcctga
     6781 agaaggtaac ctgcaatttc agacaaacgt gcctcagtat aacgcatagc cgcaggagga
     6841 tctccgtcca tagaaccgtt attaccgtgc atttcaacta gaatctcacg atttttccag
     6901 ttctgtgaca tacgaaccat ggcatcatag atagaagaat ccccgtgtgg gtggaaattc
     6961 cccatgatgt tcccgactga cttggccgac ttacggtagc tcttgtcaaa agtattgcta
     7021 tccttattca tagaataaag aatacggcgc tgaaccggct tcaacccatc acgaatatct
     7081 ggcaaagccc ggtcttgaat aatgtacttg gagtagcgac caaagcgctc tcccatgatg
     7141 tcctccaggg acatgttttg aatgttagac ataagataca aagcccataa aataccaagt
     7201 gaaaatagaa aattcttgaa gtaagcaaac tcacaagaga atttatcttt ttcacacagt
     7261 atctagggcg tgttcaactc ctttcaaaga atgtagagta ggtttttatg cagtaaaaga
     7321 tattttacgg gaattcctcc cgtgttcagt tacgataagt aaccaaacta tcctgtttgt
     7381 atttttcaat atgaaaatct ggttttccaa aattagtctt agtttgtgtc ttagccgctc
     7441 ccttaagcgc ctctttgaga taagcactca tagcagattc ttcattaata atcctgcaat
     7501 tttttcaaac caagattttc aaactgcttt ttcacatagt cattcacatc cgactctaat
     7561 ttccagttta ctaacatatt attttctttc attaaaacac tgtcgcttct tctagcgtaa
     7621 acttgacatt atcttcaatc catttacggc gtggttctac cttatctccc atgagaacat
     7681 tgacgcggcg ttcggcgcgc gctaaatctt caattgtgac acggatgagg gtacgtgttt
     7741 ctgggttcat ggttgtttcc cagagctggt ccgcattcat ctcaccaagt cctttgtatc
     7801 gttggagggt agcgccttta ccgaactgtt tacggagttc ttctagttct ccgtccgtcc
     7861 aagcgtaggc cacttcttct ttcttgcctt tacctttgga catcttataa agaggtggga
     7921 gggcaatata gacatgacct gcctcgacta gcggacgcat gtaacggtag aaaaatgtca
     7981 agagcaaggt ctggatatgg gcaccgtcgg tatccgcatc ggtcatgata atgatcttat
     8041 catagttggc atcttcaata gagaagtctg ctccaacacc cgcaccaatg gtataaatca
     8101 tggtattaat ctcttcattt ttgaggatat ccgccatctt ggccttggct gtattgacaa
     8161 ccttaccacg aagaggtaga atagcctgga acttgcggtc acgaccttgt ttggcagaac
     8221 caccggcaga gtccccctca actagataga gttcattctt agcaggattc ttagattggg
     8281 ctggggtcaa tttcccagac aacaagccct tatctttctt gtttttcttc ccatttcggc
     8341 tctcatcacg cgccttacgt gctgcttcac gagcatcacg ggccttgata gccttgcgga
     8401 tgaggttaga agctaattcc ccattttcca taaggaaaaa ggtcaactta tcagccacta
     8461 ttccatccac aactgggcga gctagggggc ttcctagttt atccttggtc tgtccttcaa
     8521 actgcaagtg ttcttcagga actaagatag aaagaacgac cgctagtccc tcacgatagt
     8581 ctgaaccttc aaggttttta tctttttcct tgagaagacc tgttttacgt gcatagtcat
     8641 tcatgacctt ggtaatggca gacttgaatc ctgtctcgtg cgttccaccg tccttggtgc
     8701 gaacgttatt gacaaaggat agaatgttat ctgagaatcc gtcattgtac tggagggcta
     8761 cttccacttg aaaaccattg tcttcccctt caaagtaaag aactggcgtc aagatttcct
     8821 tatcttcgtt gagataagaa acaaaatctt gtactccatt ctcatagtgg aactcaatcg
     8881 cttcatctgt tcgcttgtcc gttaaagaca aggtcacatt tttcaagaga aaggctgatt
     8941 cattaaggcg ctctgaaatg gtattgtact tgaaatctgt cgtagaaaag atagtcgcgt
     9001 caggcataaa agtaactttg gtgcctgttt tagacttggg tgctgtaccg attttcttca
     9061 aagtcgtgac aggttttcca ccattttcaa aacgttgctt gtaaactgcg ccatcacggg
     9121 taatttcaac ttctaaccag ctagaaaggg cgttaacaac ggaagaaccc actccgtgaa
     9181 gtccacctga tgtcttatag ccaccttgac cgaatttccc tccggcatga agaatggtaa
     9241 agataacctc aacagttgga attcccatag cgtgcatacc tgtcggcatc ccacgtccat
     9301 ggtcttgaac cgttagacta ccgtctttat tgatagttac atcaatacga tcaccaaacc
     9361 cagacaaggc ttcatcgact gcattatcaa cgatttccca aactaggtga tgaagaccag
     9421 cgccatcggt cgatccaata tacatccctg gacgttttcg gaccgcatcc aacccttcta
     9481 gcacctgaat agcatcatca ttataattgt taatattgat ttcctttttt gacacaagga
     9541 acctcctatt cgttcatctt tactattcta caggttttcc aaggattttg caaaattttt
     9601 ctttctccga tgtgacaatt tcagcagaga ttctctgctt ttctttccca attcatgata
     9661 taataggagt atgattacaa tagttttatt aatcctagcc tatctgctgg gttcgattcc
     9721 atctggtctc tggattggac aagtattctt tcaaatcaat ctacgcgagc atggttctgg
     9781 taacactgga acgaccaaca ccttccgcat tttaggtaag aaagctggta tggcaacctt
     9841 tgtgattgac tttttcaaag gaaccctagc aacgctgctt ccgattattt ttcatctaca
     9901 aggcgtttct cctctcatct ttggactttt ggctgttatc ggccatacct tccctatctt
     9961 tgcaggattt aaaggtggta aggctgtcgc aaccagtgct ggagtgattt tcggatttgc
    10021 gcctatcttc tgtctctacc ttgcgattat cttctttgga gctctctatc ttggcagtat
    10081 gatttcactg tctagtgtca cagcatcgat cgcggctgtt atcggggttc tgctctttcc
    10141 actttttggt tttatcctga gtaactatga ctctctcttc atcgctatta tcttagcact
    10201 tgctagtttg attatcattc gtcataagga caatatagct cgtatcaaaa ataaaactga
    10261 aaatttggtc ccttggggat tgaacctaac ccatcaagat cctaaaaaat aaaacgccag
    10321 ttctgtactg accccaaaaa gttagacaat taatttatcc gaaggattta gttctgtatt
    10381 gcacagggct aagtcctttt agttttacct taattcgttt attgttgtag taatcaatat
    10441 agtctacaat ggcttgttcc aattgcttaa gcgactgaaa cgacttctca taaccgtaaa
    10501 acatttccga tttcagaatc ccaaagaagg actccatcat accattgtct gggctgttac
    10561 ccttacgtga catggatgct tgaattccct taccctcaag aaactgatga taagaatcgt
    10621 gttgatattg ccagccttgg tcactatgga gaatcgtatt ctcgtagtgc ttctctgtga
    10681 atgcctgttc caacattgtt tgtacttgtt ctaagttggg tgaagttgaa agattatagg
    10741 cgataatttc gctgttaaaa ccatctaaaa ctggtgataa gtaaagcttt tgagtactta
    10801 ctggaatggc aaattcagtc acatctgtgt agcactgttc cattgtttta gagccttcaa
    10861 attggccttg aatgagattc tctgctttct taccaacgtc tcctttatga gaagaatatt
    10921 ttcgtttctg tcgcatttta gcttgtaaat tgagtacttt catcaagcct tgaactcttt
    10981 tatgatttac cagataacca cgatttctta gttctaaata aatccgacga taagcataat
    11041 ttcccttgtg ttcgataaaa atggattgaa tttcagcttt aagctcttgg tccttatctg
    11101 gtttgtctag ctgtttcaag tgatagtagt aggtcgaacg agctagttta atggctttta
    11161 gaagaatatc taacgaaaac tcagtcatta attcttgaac aatttctgtc tttcttcttt
    11221 ctctttttcc tccttcaatc ggagttctct taactttttt aggatggcat tctccgctct
    11281 caggtactca ttttctgctt gaagatgttc taattctgtc ctctcttcag gtctcttttt
    11341 tggcttacgt cccattttag gtactctccc tcttgttttc tcaacaatag tatacccgtt
    11401 tttttctgta ttgtgctagc cagttaagaa gtatcgtacg acttgggaga ccgtattcaa
    11461 gagaaactct gtctttagtc cagccttcat gtcagacttt attaat
//
LOCUS       DAVLVF010000056        11395 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVLVF010000056 DAVLVF010000000
VERSION     DAVLVF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11395
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.056"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..675)
                     /locus_tag="VYN66_001433"
     CDS             complement(<1..675)
                     /locus_tag="VYN66_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495444.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTR"
     gene            complement(685..1437)
                     /locus_tag="VYN66_001434"
     CDS             complement(685..1437)
                     /locus_tag="VYN66_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495445.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(1437..2201)
                     /locus_tag="VYN66_001435"
     CDS             complement(1437..2201)
                     /locus_tag="VYN66_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495446.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(2297..2700)
                     /locus_tag="VYN66_001436"
                     /pseudo
     CDS             complement(2297..2700)
                     /locus_tag="VYN66_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2880..3512)
                     /locus_tag="VYN66_001437"
     CDS             complement(2880..3512)
                     /locus_tag="VYN66_001437"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV1495447.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(3546..4247)
                     /gene="pyrF"
                     /locus_tag="VYN66_001438"
     CDS             complement(3546..4247)
                     /gene="pyrF"
                     /locus_tag="VYN66_001438"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV1495448.1"
                     /translation="MREHRPVIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGAAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHTIKDEWTQDWN"
     gene            complement(4480..5679)
                     /gene="divIB"
                     /locus_tag="VYN66_001439"
     CDS             complement(4480..5679)
                     /gene="divIB"
                     /locus_tag="VYN66_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV1495449.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEDDKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDLDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(5689..6747)
                     /locus_tag="VYN66_001440"
     CDS             complement(5689..6747)
                     /locus_tag="VYN66_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV1495450.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQTHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLLELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(6751..8103)
                     /locus_tag="VYN66_001441"
     CDS             complement(6751..8103)
                     /locus_tag="VYN66_001441"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV1495451.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLVKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(8207..8848)
                     /locus_tag="VYN66_001442"
     CDS             complement(8207..8848)
                     /locus_tag="VYN66_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495452.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(8850..10871)
                     /locus_tag="VYN66_001443"
                     /pseudo
     CDS             complement(8850..10871)
                     /locus_tag="VYN66_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            complement(10954..11253)
                     /locus_tag="VYN66_001444"
                     /pseudo
     CDS             complement(10954..11253)
                     /locus_tag="VYN66_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
BASE COUNT         3611 a         2459 c         1937 g         3388 t
ORIGIN      
        1 acgagtgaaa tagaaaatac ttttaaaaag aaagactaaa aatagcaact gaagaaataa
       61 atctcgtcct atatctccat tccctcgatt caaaatataa aatagataat tagttcgatt
      121 tcctacaaat agaccaccaa acacaatcat gagtaaaaag aaactaacgc aagcaaagtt
      181 catttgtaca ttcgctccta gtgcgtataa cataaagcaa aacaagacta acacacaagc
      241 attcaaagaa tcaactataa acaaaactcc tatatcccag tttggtaact gtaacataaa
      301 ttgaatcact agaactttta ccatgattaa aatgagtcca taaagaaaac aggtccaaat
      361 ctgataccta atccagagct taatagaaga aaatcgaatc acttccataa agaaatcgga
      421 tgctcttcta cgatatacaa ctgctagaaa aaatgtatga ataataaacc aatgaagcaa
      481 ccatatagat aagactaaaa aagagttttg actcagatta ggccctttta aaatttctat
      541 aacaagtcta tcatttcctt ctaaccaata ccataatcct aaacaaatca atatccaaaa
      601 aagaacataa ccccaaaagt acttggtata gattatctga ttcaacctcc acatgtgact
      661 acttaacttt cccatttcca acttttacac tctcctccag tttctatgaa cagaaaatac
      721 agtcaatgga attatgatag atagtaatgt aagccaataa atcaatgcat acggattgga
      781 atttaccata tactgaaaaa ttggcaataa tccaattatc ccattgtcca actgataaat
      841 ccagaataag gattctacaa ataaaaaagt aaacagaaat actaagctca tgtttgattt
      901 tttaaaccaa gaaaaactgc taatagtaag taaggataaa aagaaatagt atgctatata
      961 agagaaaaaa aatcctataa agaaactagc attgtttgca atacttatac cataaaattc
     1021 tcttaaaaaa tcaacctcct ttatctccaa agagaagcta aaaccattac taaatgcaat
     1081 tagaaaaatc aataaaaata aagtcgccgt ccaaatcccc gtactaagag ctgctaattt
     1141 gaaactaaaa ctggtaaagt gcttaattga tttcagacga atgcgacact ccaacctatt
     1201 aaaatagtta ttcatcaaat aaaaaaagaa caatatatat gtgaacggaa agcaatatac
     1261 tccagtcgtc atatcttgaa gtaaaactaa gatccattct aatacatttg gatggattga
     1321 atattggcga cagcgcaata aatatactgt actagataaa acacaggata gcagtaatat
     1381 aaaataaacc aatactgata aaaaatcttt tttgtaaaat tgaacaaatt gtttcattat
     1441 acgtagtcct ctgaatgtag aaaaaatgta ccataaacaa ccaaacaact aacaaataaa
     1501 ataaaagcaa gatgcccact aactaaggaa agactgatat ctttctgata tcccaaagct
     1561 aatgttgtca caggttctaa gtaagatagc cctaaaatag cccaaaaaat accaccaacc
     1621 atcatatagg caactgggat gaaaatagct cctatttttt tcttcactag caaagcacta
     1681 gctagtccaa aaatagagaa cacagcgccc caaactccat accagagagt cgttacaaga
     1741 ctatagagaa actgattgga atcaaataat tcttttaagg caccactata atctccaata
     1801 taaatttcct gataaggagt cactaaaaga ttaattccta ataataataa ataggggaga
     1861 aaaaagacta gaaaagaaga aataattaca gtactaccca caatagccag atacttcttt
     1921 ttagaaattc gacacaattg tgctgttaga aaatgactct cagcatcctc tattatctga
     1981 ctagaatagg gcagtgtaca gataagtgca gctactaggc taatcggtga aaatcctcga
     2041 atagaagaag cggcaaaaaa aatcgataaa ccttcaattt tataaatacc attgaaagca
     2101 aggaaatttc ctaaactcat gcaaagaagg gtcaaaaata aagacatata aaatcgaggt
     2161 gattgaacga ctcgtacaag attacaaatg aaaaattcca tccttactcc tccttataat
     2221 aaaaataggg tgtagcattc ttttttcatg ctacacccac aatcaaccat ctctaaggct
     2281 tactctgaca agtaagttaa taagaatctg gattccaaga acctgaagta tgaattctta
     2341 catgatttcc aaattgtggc gccatagcta atctagtacc agaaccaata taattgtcac
     2401 cacctccatt atagtacatg acaatcctag agccagaccc caatgaatat accggggtaa
     2461 tatctgaccc actataggcg ctacgaatag aggtatttaa ccttttaccg ccaccagtgc
     2521 tgtcattatt attaattcca gcagaggcgt tttctttctc aagctcacta gttgtatccg
     2581 aaccccaacg cggcaacgtt aaatcataat ctccaatctg tctagctaaa actgtaagga
     2641 gaaacaaact agatgcaatt gtaactacgc aaaaaaggaa tatatttttc ttcttttcat
     2701 gacatcgtcc tcctaagaag tgataaacaa gatagttgat tgatcaattt gatacatatt
     2761 acattttcat tatatcaaca aaagaaagcg ttgtcaatca ttttatttaa aatattgtca
     2821 tattttaaat aaaatgataa aagaggctcg gtagcctcat ctctctttat cttactgacc
     2881 taaccttctt gccaattttc ttggtcttct ttaaagcgtt ttagaagatc aaggccctct
     2941 ggcgtgatgt aaccttcttc ttgggctaga tggataagct cgctatagtt tgaaagcgtc
     3001 acaagtttaa caccagcatc tgcaaagttc ttatctgctt ttggcaattg gtagctgaaa
     3061 atcgctacaa ctccaagcac atctgctcct tctcgcttgg ctgctgctac agcttcaaga
     3121 actgaaccac ccgttgaaat aaggtcttca accactacca ttttttgacc ttgagctacg
     3181 cgaccttcga tttgattacc agctccgtgg tcttttggtt tgctacggat gtaggcaaaa
     3241 ggcaagtcca tcttatcagc aataatggct ccgtgtggaa tccctgctgt tgcagttcct
     3301 gcaatcactt ctacttcagg aaaggcttct ttgatagctt ccacaaaacc attttcaatt
     3361 agggtacgag tttctggata ggctagtgtc acacgattat cagtgtaaat cggtgacttg
     3421 ataccagatg cccaagtgaa gggttcctct ggtttgaggt aaacggcttg gattttcaag
     3481 aggtggctag cgatatcttt agcaagtgtc atggtattct ccttttattt ttctaatcta
     3541 gttctttaat tccagtcctg tgtccattca tccttgatgg tatgataagc tgcaacagga
     3601 tcctcagctt gggtaatggg acgtcccact acgatatagt cactgccgat ttgataggca
     3661 tcagcaggtg tcatgactcg tttttgatct ccaactgcag caccagctgg acgaatccct
     3721 ggtgtcagac agataaaatc tggattggta gcctgcttga tgacttgtac ttcctgagcc
     3781 gagcaaacaa caccatccaa gccagcttca gctgtcttct tggcatagtg aatcacagac
     3841 tcttgcagac tggtttggat attttgaaac tcctgcatct gagcttctga cgttgatgtg
     3901 agctgagtta cagcgatcaa tttagcttga ctcccaaggc cttcacgcgc cgccttcatc
     3961 atctctacac caccagccgc atggacatta gtcatatcga caccaagctg agacaagacc
     4021 ttcatggctg acttgactgt attaggaatg tcatgaagtt tgagatccaa aaagacacta
     4081 tgacccaaac cttttaagta ggacacaatc tcaggccccg ctgcgtaata aagctccatc
     4141 cctaccttga gataaaggct ttcttctgct gggaaaagag ctaaaaattc cttgaccgcc
     4201 tcaaaactag gaaaatcaag agcaatgact ggacgatgtt ctcgcatagg tttctccttt
     4261 tacctttact agtgagagag tctggccaac agaagccacg aaaaaaggaa cctgccaaca
     4321 gcaaggttcc acgaaaaatg ggtagtctcc accctttcac cgtctaacct tagctgcctc
     4381 tctggactgc tttaaaggtt ttttactatt ctattatttc tactagcact tgtcaagtaa
     4441 aaacatggtc actaaagaac tataagagaa aaggtaaacc tagcgacgcg atgagcgctg
     4501 ggtcgtttgg tttcgattgc tctcttcctc ttgttttttc tgttcttctt cttgtttttt
     4561 ctcagcttcc ttagcctctt gtttggcttt ttcctcagct tccataatta atttatccgc
     4621 cacagtgtaa ctgtaaattc cagcttccat gtcaatcaca ctcggttctg acaattgtgg
     4681 cttaatctta ctataataag gcagtttctt gctcatttca gaaagcggaa ccaagacttc
     4741 gtccaaatca ttcatagtca atcgaattaa atcggatgtc accttgcttg gggctaattc
     4801 caccttttgg atagccgcct tgagttctgg gctaatttga gcaagttctg agacaaaaac
     4861 cttgatttgt tcactatcat taaagagaac tgataaataa gtttctggta aactgttcag
     4921 gctcacagaa ctagtctcaa gctgaccact ggaaagaata ggataatgat tttcaccaga
     4981 aatatagtag gccacaatat catattcctt gaccttaata gtgaacttag ttggaaattg
     5041 atagacaagt tgagctgatt caacccaata gttagactta atctgctttt catattttgc
     5101 cttgtctagc agaaggttaa tcgtataatc cgaatcctga atgcctgaag cctgtcgaat
     5161 atcatcagct gtagtttgca ccgttccctc aacacgaata tctttcatgg tcgcataagg
     5221 actgagcaag taggcagaga caatcaataa aagcagactt ggaaataaaa tcgtgaaggc
     5281 tcgcaagata tggataccag gaatctttgc tttggctggt ttttcctttg tagccttttt
     5341 agcaagcttt ttatcctgtt cctccttctc tttagactct ggttcttctt tctcttcttt
     5401 ctctttgtca tcctctgagg atgctacttt ttcttcagac tcttccttag ctgattctga
     5461 atcttcctgg tctgtttcac tctcctggtc ctgtttgtcc tctgacttct cagattcttc
     5521 tcccatccga gcttgtcttt ccttttcctt ctcctcagct agagccgctt cttcttcagc
     5581 cttctttttt agatattctt ggtttcgttt ctgccattct gataactctt tcaattcttc
     5641 gaggatttct ttgtcctcat ttttcttatc ttttgacatt tactttcctt atgataaatc
     5701 ttttttcaac aattgataaa aatctgctag agatttcaat tccttagaag ctttcatctt
     5761 agcttggtaa tcttccttgt gacttagtaa gtgagaaagc ttctcttcca aactatccaa
     5821 ggtcaaatcg ctttcttgaa ggtcttctgc atagcctttt tttacaaagt aagctgcatt
     5881 ttcaatctgg tcaccacgac tagcttcacg accaagcggc acaatgacat gcaattttgc
     5941 tatcgccaag agctcaaaaa tcgtattggc accacctcgt gtcacaacaa tatcagccaa
     6001 ttccaacaag ggttgataga gatcggtcac atagtcaaca cgaaaaagat tttggctcaa
     6061 ctcattcagg ctagaatctc cagttagatt gataatattg tagcgctctg ttagttcttt
     6121 cttatggtct gtcaccaatt ggttaaagac acgagcacct gcagaaccgc caacaaacaa
     6181 tacagtcggc aatttatgat taaagtgggt ttgaatatcc accaattcat ctggttctgg
     6241 atttttttga tctgaaacct tggtcactgc tcccacatgc tcaaccttag acaaactcga
     6301 agcttgctca aaggttgaat acatcttagt cgcaaattta taggcgattt tattggccaa
     6361 gcccatagac aggtcagatt cgtgaataaa gacaggcact cctgacacac gcgctgcgat
     6421 aacaggcggt actgagacaa agcccccctt tgaaaaaagg gtctgtggac gaagtcgcaa
     6481 catgataaag agcgattgga cgattcccca gccaactttg aagacgtcca gcatattttg
     6541 ccaagagaaa tagcgacgca atttcccagt cgcaatggag tggaaagtga catccaaacc
     6601 tgacttaagg atttcttggt gttcgatacc acgcttgtcc ccgatatagt ggacttccca
     6661 accatcttcg atgaacttgg gcattaacaa aagattgagg gtaacgtgtc caaccgtccc
     6721 cccacctgta aagacaattt ttttcatatt ttattctttt aactccgcta ctgtgtcgat
     6781 aaagaggtcg ccacgtactt caaagttagc atacatatcc cagctggcat tggcaggact
     6841 aagaagaacc acatctcctt gagtcgcaag ctcataggcc ttgcgggtcg catctgcaat
     6901 atctgtcgcc tccacataag cgacaccagc cttgtctgct gcccgtttga cacgttctgc
     6961 agattgaccc aggatgacca tcttcttgag tccagtaatg tctggcacca attcgtcaaa
     7021 ctcattgcca cggtccaaac cacctgcaat caagacgacc ttgctgttgt caaatcctga
     7081 taaggctttt tgagtagcca agatattagt tgatttactg tcgttataga atttaacacc
     7141 cttgatgtca tccacaaact ggagacggtg tttgacacca ccgaaggctg aaagagtttc
     7201 cttgatggtt tgattgtcca catcacgaag cttggctaca gcaatagtcg caagggcatt
     7261 ttccacattg tggctacctg gaacaccgat ttcattcgct gccatgacta cttcaccacg
     7321 gaagtagagt tgaccatctt ccagataagc tccatcaacc ttttcaagtg ttgaaaatgg
     7381 tacaacagtg gcttctgtct tggaagccaa gtcttttacc aagtcttgat taaagttcaa
     7441 gacaaggaaa tcagctgctg tcatcttgtt ctggatattc cacttggctg ctacatattc
     7501 ctcaaataat ccatgataat cgatatgagt tggcatgagg ttggtaataa ccgcaatctc
     7561 tggatggaat tcttgaacac ccatgagttg gaaagaagaa agttccataa caagcgtgtc
     7621 cttatctgat gctatttgag caacctgact ggcaggatag ccgatattcc ctgataaaag
     7681 accatgttgc ccagcagcag tcaaaacttc cccaatcata gtcgttgtgg ttgtcttacc
     7741 gttcgatcct gtgataccaa taatcggtgc ttctgaaatc aaataagcca attccacctc
     7801 agtcaagact ggaattccct tggccaaagc cttttcaatc atgggattgc tgtaggggat
     7861 acctggattt tttaccatga gggcaaactc ttcatccaag agttccaaag gatggccacc
     7921 tgtaatgacc ttgatccctt cttccagcaa acattgggca gctggattgt cctcgaatgg
     7981 tttcccatca tttactgtca caatggcacc taacttgtcc aacaaacgag ccgcagattc
     8041 accagacttg gccaaaccta aaacaagaac tttcttattt ttaaattgat ctattacttt
     8101 catgtctcga actccatttc tactcctact attttaccat ttttatggaa ataaaaaagc
     8161 cacaaagtgt gtttgtgact ctttcttcta actgaatctt accatatcat ctatgtgata
     8221 aatcggtaac tcgaatgacc tgatccactt gctcccaaat cagaggattg tgggtcgcaa
     8281 taataatggt ccgattcgga ttttttaaag attctaggat ggaaagtaat tcctcagaat
     8341 ttttggggtc taaggaagcg gttggttcat ctgcaagaat caaaggcgga tcctttaaaa
     8401 ttatcttcgc tagtgcaaca cgttgtgctt ctcctcctga taactcaaat ataggttgct
     8461 tcaaatccaa ataagagagg tttacacagt ttagagcttg tttcatcaaa gagattttct
     8521 ctttttcctt caacttttta ccaactaaac ccagattgag attctctttg acggtttggc
     8581 tttcaattaa gccaaaatct tgaaataagt atcctaagta atctctaaag aaaacagaag
     8641 gcttgatgtc cttaagagaa gtgccatcat agatgatttg ccctttgtca tatggctcca
     8701 atcgtccaat catattcaag agtgttgtct taccacagcc acttgtaccg attaaggcat
     8761 aaattttccc accttcaaaa tgaagattcg tatctgaaaa tagctgacgg cttccaaatt
     8821 ttttagatat attctttagt tcaatcatcc tattttcctt tcataattgt catagaaaca
     8881 cgagattctt tctgcgcttg acggtaaagc gtcaaaactg cactagctag aaagaccaat
     8941 aaagtgagca agccaatcac caagtctcga ctgcttaaaa taaagagact agcaccaaat
     9001 acaaaactgg caaattggct aaccatatac tgagcatgtg tttcaaaaaa tcgtaaacct
     9061 gaaattcgtt taatcaagat atctcggcgg aattgctcga aatatagaag attgacagaa
     9121 taaaatagta ataaggaact agctatcccc acaatagctc ctaagattaa agttgctgtt
     9181 tcagtttgaa cttcattata acgagttaga taaacacttc ttccttcttt aagataggat
     9241 acttgctcat aaattccagc tttcttcaag agttctagcc cactctcata tcctttgata
     9301 aagagttgtt ttccagcatt gatagaccaa ctagataagg atataaaact atcacctgta
     9361 gaagtcggcg tgaataccac taaaatcgga tcagtcaaat actgagtaga tacgggattc
     9421 tcaccgttat tataaacaaa ccgcttttct cccattgaaa gataactaac gtgcgctttc
     9481 atctcataat ccaaaggagc acttgcctcc tcaccagatt ttccataata actcaatctt
     9541 tcttcaaaaa ctttcttaag ttctgcttct cgagagcgca aatgttctgg gagcaagagg
     9601 ataaactcac ctttttggag atgggctaac ttctgtttgg tctcagcatc taccacgacc
     9661 ttttccttgt ccaaataact gggactaaca tagagcgtat tagcatctgg actataggta
     9721 tccagtgtct ctccctgttc attttttcct tgtggattgg caaaatggag cagattatcc
     9781 tttacataaa gagcttgttc ctcttcgatt gcttccttgg caaaggcata ccacttgctc
     9841 tgattttctg tatcttttcc tctatcacct aagccaaaag aaatttgata atagtctgct
     9901 ctatcctgcc atgcttgttt tgaaatttca agttctttca atcgttggta agacgtcaaa
     9961 cctgtcttaa cagcgtatcc tactgtaaag acagcaacca actgacacaa tagggttaaa
    10021 atcatcaggc gtttaagggg taatctcccc ttaataacgg gaactaatgc tttgtaactc
    10081 aaactcatta gataaagaag cattagtaaa attgaaatcg ccaataaaaa caacagatag
    10141 aaactaatcc caaaaccata ggtggctaac aagataggat aaaacaaacc ttgactaaaa
    10201 agaacgactc ccccacctag gaaggaaagg agggctgata gaaggagcta tttgatatca
    10261 gtagataaag aatgccccat gatggataag agagtctgac cagaaaagag ttttatacct
    10321 gctgctctca tttccttaat ccgagtgata atcactaaag caaagaaaga taagccaaat
    10381 attgctaaac taattaaaat aaggggattt agtaatattc gaaaagcaag aaaatagggc
    10441 ggtatctttc ggtcagcact tgctttataa cccaaatctc ctaatttatc ggcaagcttt
    10501 tctttcgtca aggagcctga caaaaggaga taactattta gcggatcact acgttcacga
    10561 ctttcttggc tagcttcttg gaattctttt ggtaaagttc cctgaccata agttgcataa
    10621 gtaaagtgag tcgtcccatc cttactcggc tctacaattc ttctagctat taaactctgt
    10681 tctgagtttg caaaattctc caattcctgt tcaaatacct cacgcgtcgg ttcctgagta
    10741 tcttttttga cacgaagtaa agaaacggaa tcatagcttg catataaata ttgtggcgca
    10801 cgtaagacaa taatccaagc aaggaagaag ctgagaaaaa aagttgataa taatatgaat
    10861 agtttcttca tagtagactc cttggtagac tccttgtaaa caaaattccc cctgtaattt
    10921 cttacaaggg gaacgattta aatcaatgaa cgattagtca taatcacagt aaaatgctac
    10981 ttgttctccc catttagtcc aaatccatgc ataggaggtt tttccagcag gagcttctcc
    11041 tttatcagaa tgaccatccc atctgctcac gatggatgaa taatgatatt ttttaccatg
    11101 atagtaattt gaaaaagcct aaccacctcc tgaaccttct ccatatgtcc atactcctcc
    11161 atctggatat tatacagcag ctgatgcagc tcccaataat gtaaaacttg aaataagagc
    11221 tagagcaagt aatctatgtt ttttcgtttt cattttattt tttctttcaa aaaaatcaca
    11281 ccttgagcaa caatgcaaca aaataaatcc tcctctctct tttattgaaa ccgctttctt
    11341 atgtgataag aataacattt ttattatttg ttgtcaagga aaaaatcgaa ttttt
//
LOCUS       DAVLVF010000057        11181 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVLVF010000057 DAVLVF010000000
VERSION     DAVLVF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11181
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.057"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            34..1029
                     /locus_tag="VYN66_001445"
                     /pseudo
     CDS             34..1029
                     /locus_tag="VYN66_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1055..1699
                     /locus_tag="VYN66_001446"
     CDS             1055..1699
                     /locus_tag="VYN66_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV1495453.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFTAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(1741..3729)
                     /locus_tag="VYN66_001447"
     CDS             complement(1741..3729)
                     /locus_tag="VYN66_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1495454.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVFRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFTKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3731..4489)
                     /locus_tag="VYN66_001448"
     CDS             complement(3731..4489)
                     /locus_tag="VYN66_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495455.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4917..5108
                     /locus_tag="VYN66_001449"
     CDS             4917..5108
                     /locus_tag="VYN66_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495456.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(5462..5734)
                     /locus_tag="VYN66_001450"
     CDS             complement(5462..5734)
                     /locus_tag="VYN66_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1495457.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(5793..6242)
                     /locus_tag="VYN66_001451"
     CDS             complement(5793..6242)
                     /locus_tag="VYN66_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV1495458.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            complement(6229..8358)
                     /locus_tag="VYN66_001452"
     CDS             complement(6229..8358)
                     /locus_tag="VYN66_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV1495459.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
     gene            8402..8485
                     /locus_tag="VYN66_001453"
                     /pseudo
     CDS             8402..8485
                     /locus_tag="VYN66_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            8474..8614
                     /locus_tag="VYN66_001454"
     CDS             8474..8614
                     /locus_tag="VYN66_001454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495460.1"
                     /translation="MDKTDEVSSKHCFEVVDRTDEVSSKHCFEVVDRTDEVSNRTTVR
                     RS"
     gene            8860..9765
                     /locus_tag="VYN66_001455"
     CDS             8860..9765
                     /locus_tag="VYN66_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV1495461.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            9762..10577
                     /locus_tag="VYN66_001456"
     CDS             9762..10577
                     /locus_tag="VYN66_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV1495462.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTNLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(10707..>11181)
                     /locus_tag="VYN66_001457"
     CDS             complement(10707..>11181)
                     /locus_tag="VYN66_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1495463.1"
                     /translation="LILLDGGYLDLDKILPLDTELEETKNYIKSQIISDLDVLTSKEK
                     SEAKHWSENMEKAVRQSYHWNVEYNRYELAINYENIEAILRLRRKIQAFKREVGDTLF
                     ISPRYPNEATWREEALKELPDYFDTIFLENFGHELYTQAPKEIASLMNEWLAYFL"
BASE COUNT         3353 a         2343 c         2156 g         3329 t
ORIGIN      
        1 ttgttcaacc acaaacacaa gaaaggcaca gagatgcaag acaattatac tacaaaagcc
       61 aaacatttga caatcgatag ccgtcgctta atcgaaagat ggaaaaaaga aggaaaatca
      121 aatagagaaa ttgcctctct acttggaaaa gctcctcaaa ctatccacac tgaaatcaag
      181 cgtaggacag tccgacaatg tcttggaaaa gggcgcttca aagaggttta ttctgccgac
      241 tatgctcaac agtcttatga aaataatcgc aagcactcgg tcaagagatc aagcgtgacc
      301 aaggaactaa aggaaaagat tctccactat cataaccaaa aattttcgcc tgaaatgatg
      361 gttatggcta aaggggttaa cgtgggaatt tcaaccattt actattggat tcatcatgga
      421 aaattggggt taagcaagca ggatctgctt taccctagaa aaggaaaagc gcttaagaaa
      481 caggctagca ccaactttaa acctgctggt caatccatcg aacagcggcc tgaagctatc
      541 aatcttcgct tggagaatgg gcattatgag attgatacgg ttctacttac gagatcgaaa
      601 aactactgct tgcttgtctt gacggatcga aagagtagac atcagatcat ccgattgatt
      661 ccaaataaaa gtgctgaggt ggtcaatcag gctctaaaac tcatcttaaa acaacacaag
      721 attctttcca tcacggcaga taatggaacg gaattcaatc gcttgtttga tatattttct
      781 gaggagcaca tctattatgc gcacccctat gcctcttggg aaaggggaac taatgagaat
      841 cacaacaggc tcattcgtag atagttacct aagggaacca agaaaatgac tcccaaagaa
      901 gtcgcattca tcgaaaagtg gattaacaac tatcctaaaa aatgcttgga ctacaagtca
      961 cccagagaag acttctggat ggctaacttg aacttgaaat ttagcgtcag aaacaaatca
     1021 cgaaattgat tcaaattttg caggtcgttt aaatatcctg cgtgcgggtg ttcttgatgc
     1081 taacgatgga attatttcca ttgctggtgt ggttatcgga gttgccagtg ccacgaccaa
     1141 tatctggatt atctttttat caggttttac ggctatctta gctggtgcct tttcaatggc
     1201 tggtggagaa tatgtatccg tttcaactcc aaaagatacc gaggaagctg ccgtttcgcg
     1261 agaaaaactc ttgctagacc aagataggga actagccaaa aaatccctct atgctgctta
     1321 tatccaaaat ggagaattca aaacttctgc ccaactcttg accaataaga tctttcttaa
     1381 aaatccactc aaggctctgg tagaggaaaa atatgggatt gagtatgaag aatttaccaa
     1441 tccttggcac gctgccattt ctagcttcgt tgcctttttc cttagaagtt tgcctccaat
     1501 gctgtcagtg accatttttc caagtgatta ccgcatccct gctaccgtcc ttattgtcgg
     1561 tgtggccctt cttctcactg gttacacaag tgctagactt ggaaaagccc caaccaaaac
     1621 agctatgatt cggaaccttg ctattggtct cttgaccatg ggagttacct tcctgctcga
     1681 acaacttttc agcatttaga atacaagaaa tacctcgatt ttgaagtcga ggtatctttt
     1741 ttacatttgc acaatcttgc gataacttct tgaagtaatc atgaaaatca gcacataggc
     1801 gatgaggaag atagcgcaga tagacaaggt cacaatcaac atcatagtcg tatccagtac
     1861 accaatcact tttaaaatca ggctaagcat atggtaggca aaggcgagat gtatgaaggc
     1921 aaagagcaaa ggaaggaaga aaacagttaa aacctgtttg ttgatggttt gcttgatttg
     1981 cttttggtcc aaaccgactt tctgcaagat aataaagcgt tcacggtctt cgtagccttc
     2041 agaaatttgt ttgtagtaga tgaccagaac agttccgacc ataaagataa tggataggaa
     2101 aataccgata aagaagacac caccaaagag ggcactcatc tgagaactag catctgctag
     2161 attgctacca taaacatagc taccttctgt gtctaattga gcattaaatt gattgaggta
     2221 gttttcatac tcctcagcga ccttgagttg ttcttcttcg ctggcatcta cattcatacc
     2281 accgtaaaac tgattataga tagccgaatc tgggaattga tccaaaaagg cttgtaaatc
     2341 aggaacaaca aggtaattgt aatcagtagt caagatatta aacttatttg gaacatggtt
     2401 gacaataaaa tctttagtaa attcttcttt gacagaaaat tgatgatcat ttagagttag
     2461 agctttctgt cctttcagtc cgtcattttt ggcaaagaga ccgacctcat ttcctgatag
     2521 agacagtttt tgaccagtca tattttcata atctttttgg tcaaatacca tgaaaactgt
     2581 ttttggttgg acacggtttt gtcctttttc aaaaatagtt aacttggttc cttcttgatt
     2641 cgcaacagca aagtaagtgt aacgaaatac ttctttttct ttaatcttat aacctttgtc
     2701 acttgcaaac tggctcaaga gtttgtccaa gtcttctttt tcaacatttt gccctgaaac
     2761 cccaaaatca tgaggattta gaactttttt aaagctttct gaggaattga aaatgcttgt
     2821 cgctgctgac atggttacca aaaccattgt tgacaagata gcgatggtgg ctagtccaac
     2881 tgcatttttc ttcatacgga aaatcaagtt agaaacagat atgaggttat ttggttggta
     2941 atagtatttc ttatttttct ttaagatttg gaggaaaacg gtaatccctg cattaaacaa
     3001 gagataagtc ccaaagataa ccagtaaaac agctatgaag aaggttgtta aggctgtaag
     3061 aggatctttt accgtaaggg caagataata gccaattcct aaacttatgg aaccaagaat
     3121 ggtttggaga ggaaggaagc gacctttttt ctctccacta gctttctcac gagagagctg
     3181 gagggcattc atacgggcga ttcgaagggc attcaggaac atgaggccta ggaaaatcaa
     3241 accgaagaca acaagcactg taatgacaac tttcgtctgg aaggtagcaa ccagctcaac
     3301 cttcaatttc attagtttga gcaggaaagc gaaaattaac ttgtcaaaca aggctccaat
     3361 accgataccc gctccaacag ttagaatccc aaataccact aactccttaa aggtcatact
     3421 gataagatga cgcttctcca agcccaacat gccataaatt cctagttcct tggaacggtt
     3481 cttcatgaca aaactattgg catagagaac gataatggct gacgcaaggg tgacgacaaa
     3541 cataccaaat ccaagtgtag cctgaatggt tgttcctcca cggatttccg caatcttagg
     3601 attgaaggtt agagagtaaa agagataggt gacagtgact gccaagagaa cagccagcgc
     3661 aaaaggatag tagagtttgc ggtttttaat caagttcgat accgctaact tattggttaa
     3721 tcgaaacata ctaattcacc tcgcttgcca tgacagtcaa ggtatcagag atttcttgga
     3781 acatctgacg ttctgtcttc tctccacggt agatttgatt gtaaagaatg ccgtctttga
     3841 taaagagtac acgtttagct ctgctagctg ctgctgttga gtgggttacc atgagaatgg
     3901 tttggccgcg ctcattgatt tcatcaaaga catcaagtaa ggctgcagat gacttggagt
     3961 caagagctcc tgtcggttcg tccgcaagga gaatttcagg ttctgtgatg atggcgcggg
     4021 ctactgctac acgctgtttc tgaccaccag aaatctcgta agggtacttc tcttgtaatt
     4081 ggtttattcc cagattctca gctgtcacca ccaatttctt catcatctct gtaataggtc
     4141 ttcttgacaa gacaagcgga agcaagatat tgtcctttac agatagagta tctagcaagt
     4201 taaagtcttg gaagacaaag cccaactttt cacgacggaa gctagaggcc tgtgaatttt
     4261 taatggttgc ggtgtcagtt ccattcaagt aaacctgacc acgacttggt ttatccagca
     4321 tagctagaat attgagaaga gttgatttac cagaaccaga ctcacccatg atggcaacgt
     4381 agtcaccctt ttcgacggta aagtgaatat ccttgagggc ttctacttgg ttgccctgaa
     4441 aacgtgtttt ataaattttt tgaacgtgtt ttacatctaa aagtgtcatg agaatctcct
     4501 ttcttaatat cccatcaaat gaaatgtagc ttgcatcata gtgcatccca gctgaacgcg
     4561 ctcaatttct ttgtgacttg gttttctagt tttcttatgt aagatttgtt tcatgatgtt
     4621 actcctttgt tctttatgag tctagtttac atcaaaaaaa gaccgcttgc cataacctaa
     4681 ccttacaaaa gagactttaa tcttacattt ttgtaagatt gggtgaaata taagcaaaat
     4741 tacttagaaa atgatttgat ttcctatttt taaagttgta aaatataagt gtgaaaaggc
     4801 ttactttgaa aaggaggaag cgggaactaa cattttagta aggttaccta gataacatac
     4861 cattcaattt aacacttgca agtcgttcaa cctataagga aagaaggtgc gctttaatga
     4921 aacacaaaga acatatactc atcggacttc tctatctgct ctctccattc atcggtcagc
     4981 tcttagttga acacacacac tttatcggta cagaatttac cggcactgct tatgttattt
     5041 gctggttatc agttgttatc agcatccacc atttttccaa aaatgtattg tctcagcagc
     5101 aaaaatagat taaaaaactg agtttaaatt actgctttta taataaaatg aaataagaac
     5161 aggacaaatc gatcaggata gtcaaatcga tttctaacaa tgttttagaa gtagaggtgt
     5221 cctattctag ttccaatcca ctatagtttg tggatgaagc tggcgaaata ttattaatac
     5281 tattcaaaaa tcaaagtgca aactatgtca acttcacatt gccgactaaa ttacagcctg
     5341 tggctggctt cctagtttgc actttgattt ttattgagta taaaagtaaa aaagctttaa
     5401 atcaagcagt ttagactatt gatttaaagc tttttgctta ttccaaatct tctgtagaac
     5461 ctcaccaaaa ccagccttca ttgtcgtcaa tggcttgggc ttctttgcgt ttacgtgggc
     5521 ctgttccgta catttctgct tcgatttctt ccttggtagg catgatagaa gctaggatga
     5581 tatagataat cacgccaagt ccaaagtttg caactgtaaa aatagcaaac aagaaacgaa
     5641 ctagggtaac atcaaaattc cacttgtctg acagacctgc cagaactcct gaaatcatgc
     5701 gattgcgtct cattttataa aatttactgt tcatgatatt tcctctttct gctaggttag
     5761 acatagtata tcacggggag gctcggcttt catcagtcct caggctgatt tatcagaagc
     5821 aatttacctc gacaaagtcc acagcgatag cgtttggtat caatcctacg cttgcgctga
     5881 tacctttgct ggcaggattg acaacgataa agcttgagtg gtttagagtt actattgggc
     5941 aaggatggta caaaccgtaa tccatccact gctttcaaaa gttccttaaa atcccgatcc
     6001 ttgtgttgat agcccttccc ttggaaataa aggtgataat gacagagttc atgtcggaca
     6061 attttcctaa aaacctccat acccagtttc tgataaacct tgggattaaa atccagatgc
     6121 ccatctttgg ggaaaaatcg cccacctgtc gaacgtagac gcctattcca ctggacatga
     6181 tggataaaag ctctcccgaa gtcttctagt gaaacctgct tgacataatc agtcagtttc
     6241 atttggagcg aggagcgaca gattaacttt ttcacgttca gtatcaattt tcttgaccca
     6301 aacggtcacc aaatctccaa ctgccaccac ttgactaggg tgtttgataa acttgcgact
     6361 catatgagaa atatgaatca agccgtcctc gtgaatcccg atatcaacga aggcaccgaa
     6421 gtcaacaacg ttacgcacaa caccttctag cttctgacct accactaggt ctttgatatc
     6481 taggacatct tggcgaagca caggtgcgtc aaaggaatca cggaaatctc gacctggttt
     6541 gagaagatct gcaatgatat ctttaagggt ttctggaccg aggtctagct cttgagccat
     6601 ttccttgact gaaagcgact tgagtttgct ttgggcctct tcattcagat ccttaatgtc
     6661 caagcatttg aagagttcct taacggcagc gtaattttct gggtgaactc ctgtattatc
     6721 aaggatattg ctactttcag ggatacggag gaaaccagca gcctgttcaa aagccttggc
     6781 ccccaaacga ggaactttct tgatttggga gcgtgaagtg atttttccct cttcctcacg
     6841 gtatttgacg atattttcag aaatggtttt attgagtcca gctacgtgtg aaagaagagc
     6901 tgggctagct gtattgacat tgacaccaac ttggttaacc actgtatcga caacaaagtc
     6961 cagactctca gatagtttct tctgactgac atcgtgttgg tattgaccga caccaattga
     7021 cttaggatcg attttgacca attccgcaag aggatcttgc aaacgacggg cgatagaaat
     7081 ggcagagcgt ttttcaacgg tcaagtctgg aaactcctga cgagcaagtt cgctggcaga
     7141 atagacagaa gcaccacttt cattaacgat aacatagctg acttcaggga aatctttcag
     7201 aacttccgct acaaaagctt cactttcacg actggccgtt ccatttccaa tggcaataat
     7261 ctctacaccg tattgaccaa ttaaatctgc taaatctttc ttggcttctt cgatttgacg
     7321 agctgatgct ggtttaacag gataaataac ctgagttgtc agcatttttc ctgttgcatc
     7381 cacgacagct agcttggcac ctgtacgaaa ggctgggtca aacccaagaa ccacgcgccc
     7441 tttcagtgga gcaaccaaga ggagattgcg cagattgtca gaaaaaagtt ggatagctcc
     7501 ctcttcagct ttctcagtta attctgtccg aatacgacgc tcaatagcag gcaagacctt
     7561 tttcttaacg gattgctgaa caacttcatc aatataagca tttttcacct tgaaacgagt
     7621 agcaaagaag gcaagaatac ggtccgtcgc atgttcaaaa ccgatcttca agacaccaag
     7681 tttctcccca cgattgagag ccaaggtacg atagccttgc atagttccaa ctgtctctga
     7741 aaaatcataa taaatctgaa aaacctgctt ttcatcaaga ctttcatcct tggcttgaga
     7801 agtgagttta gagtgtctca gcacttcctg ataagtcata gaacgcaagg tcacatcttc
     7861 cgataaggct tcgaccaaaa tatcaactgc accggtcaag gcttccttgc cagtcgcaaa
     7921 tccttcacag acgaactttt cagcttcttt ctctaagtca actatattct gcaaaatcaa
     7981 acgagcaaga ggaaagagtc cagcttcacg ggcaatggtt gccttggtac gacgcttttc
     8041 cttataagga agatagagtt cttcaacgtc tgctaatttt tcggcaacta agatagcttc
     8101 ttccaattcc ttggtcaact taccttgttc ttgaatctta gctaagacag cttccttacg
     8161 gtcattgaga tttgtcagac ttttatccaa atcaataata gccttaatcg ccacctcatc
     8221 cagactacca gtcatgtcct tgcgataacg cgcgataaag ggaatagtcg ccccttcagc
     8281 tgtcaaactt agaacggtat caatttgctt taacgtcact cccaaatcct gagagatttt
     8341 ttcatatttt ttatccataa atctattata ccacaagctg gaaggctttc tccaatcctt
     8401 ttcatactca ataaaaatca aaaagcaaac taggaagcta gccgtaggct gctcaaaata
     8461 ctgttttgag gttgtggata agactgacga agtcagctca aaacactgtt ttgaggttgt
     8521 ggatagaact gacgaagtca gctcaaaaca ctgttttgag gttgtggata gaactgacga
     8581 agtcagtaac cgtactacgg taaggcgaag ctgacgtggt ttgaagagat tttcgaagag
     8641 tatcaactat cgaaaacaaa ttaaaaaact cccttttccg caaaataata gtataataga
     8701 ggtagaattt agaatcgagg tacacctatg gctgtcaaat ttacaaaacg agacgacttg
     8761 gacaagatgt ttgaagagtt tgctaaactc cctgatttga aacaagttac tttccctgat
     8821 gacaaagaga aaaaagtcaa agcagaaaag aaaaactaga tgactgcttt tcaacaactc
     8881 ccatctagtg tacttcaaac tggagccatt tttctctcca ttatcattga agcccttccc
     8941 ttcgttctga taggaagcat tgtctcaggg ctgattgaag tttatatcac acctgacaag
     9001 gtttatcatt ttctccctcg aaatcgttgg gggagaatct tttttgggac ctttgtcggt
     9061 atacttttcc cttcttgtga atgtggaatc gtccccatca tcaatcgttt tctggaaaaa
     9121 aaggttccaa gttacacggc cgttcctttt cttgtgacag cacctgttat caatcccatt
     9181 gttctttttg cgacctattc tgcctttggc aactccttcc atgtcgccct attacgagct
     9241 ctgggttcca ttcttgtggc tgtaatacta ggaatttttc taggattttt ctggcaagaa
     9301 ccgattcaga aagaaaatcg tctggcttgt catgagcatg atttttctta cttgagttct
     9361 gcaaaaaaag tttttcaagt ctttgtgcag gccattgatg aattttttga tacggggcgt
     9421 tatttggtat ttggctgcct ctttgcttct ataatacagg tctacgttcc gactcggatt
     9481 ctgacctcta tcagtgcgac ccctcttttt gccatcctgc tcttgatgat tttagccttt
     9541 cttctttcgc tctgtagtga ggcggatgcc tttataggtg cttctcttct ctcgagtttc
     9601 ggtttggcac cagttctggc ctttctcgtc attggtccaa tgctggatat caaaaatatt
     9661 ctcatgatga aaaattactt gaaagcacga tttatcagtc acttcataac aattgtaact
     9721 cttgtcgtct tagtctattc tctcttgatt ggagtcatcc tatgattcga tttttagttt
     9781 tagctggcta ttttgaactg actatttacc tccatctgtc gggcaaacta aaccagtaca
     9841 tcaacatgca ctattcctat ctggcctata tctccatggt gctttctttt atcttggcta
     9901 tcgttcaatt gtatatctgg atgaagcaag tcaaaaccca cagtcatctg aacagccgat
     9961 tggccaagat gacgagtatt tctcttctgg ctattccact tgtcatcggc ttaactttcc
    10021 caactgttag cttggattct cagactgttt cagctaaagg ctatcatttc cctctatcgg
    10081 aaggaacgaa tctggctatt cagacaagtg aagggactac aagccaatat ttgaaaccag
    10141 atactagttc ttatttttca aaatcagcct atgaaaagga aatgcgaacg gcggcggata
    10201 aatacttatc ccaagacagc attcagatca ctaatgaaaa ctatatggaa gtcatggagg
    10261 ctatctacga ctatccagat gagtttgagg gcaagacaat ccagtttaca ggctttgtct
    10321 ataacgaccc cagtcatgcc aatagtcaat ttctgttccg attcggcatt atccactgta
    10381 tcgcagattc tggtgtctat ggattgctga ccaagggcaa tacccggcag tatgaaaaca
    10441 acacttggat ttcagccaaa ggaaaactgg tcaatcacta ccataaagaa ctcaaacaaa
    10501 accttccaac cttggaaatc gacagcttta ccaaagtcga taaaccagaa aatccctatg
    10561 tatatagagc tttttaagaa aatcaagata aaaacgaaca agttctcttc tgaataacag
    10621 aaaaagagcc tgttcgtttt ttggttatat gaaaattagt gacttgtaga ttttcatctt
    10681 ataccatttc cagcaataca agtagctcat agaaaataag cgagccactc attcattaga
    10741 ctagcgattt ctttaggtgc ttgagtataa agctcatggc caaagttttc taaaaaaata
    10801 gtatcaaaat agtctggcaa ttcttttagg gcttcctctc tccatgtagc ttcattagga
    10861 tagcgaggac taataaacaa ggtatctccc acttctctct taaaagcttg tatttttctc
    10921 cgtaggcgga gtatcgcttc tatattttca taatttatag ccaactcata tctattatac
    10981 tcaacattcc agtgatagga ctgtcttaca gctttctcca tattttctga ccaatgcttt
    11041 gcttcagatt tttctttaga agtaagaaca tctaagtccg aaataatttg agatttgata
    11101 taatttttag tttcctctaa ctctgtatcc aaaggtaaaa tcttatctaa atctagatag
    11161 ccaccatcca aaagaatcag t
//
LOCUS       DAVLVF010000058        11036 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVLVF010000058 DAVLVF010000000
VERSION     DAVLVF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11036
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.058"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            12..734
                     /locus_tag="VYN66_001458"
     CDS             12..734
                     /locus_tag="VYN66_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1495464.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            731..1579
                     /locus_tag="VYN66_001459"
     CDS             731..1579
                     /locus_tag="VYN66_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1495465.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1611..2540
                     /gene="psaA"
                     /locus_tag="VYN66_001460"
     CDS             1611..2540
                     /gene="psaA"
                     /locus_tag="VYN66_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV1495466.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2663..3154
                     /gene="tpx"
                     /locus_tag="VYN66_001461"
     CDS             2663..3154
                     /gene="tpx"
                     /locus_tag="VYN66_001461"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV1495467.1"
                     /translation="MVTFLGNPVSFTGKQLQGGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3250..5958)
                     /locus_tag="VYN66_001462"
     CDS             complement(3250..5958)
                     /locus_tag="VYN66_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1495468.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAS
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIITKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGIVVGETQIQFYWTYSLLALVLSWLASVLPVYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSETPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5960..6661)
                     /locus_tag="VYN66_001463"
     CDS             complement(5960..6661)
                     /locus_tag="VYN66_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495469.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANCDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6911..9322)
                     /locus_tag="VYN66_001464"
     CDS             complement(6911..9322)
                     /locus_tag="VYN66_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1495470.1"
                     /translation="MIRNKKQDYVLAYKQPVSTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEANVDAFTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAVRASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(9375..10067)
                     /locus_tag="VYN66_001465"
     CDS             complement(9375..10067)
                     /locus_tag="VYN66_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV1495471.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10118..10408)
                     /locus_tag="VYN66_001466"
     CDS             complement(10118..10408)
                     /locus_tag="VYN66_001466"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495472.1"
                     /translation="MIESIRTYADYIDLYKNIFDDYFTKAEEGFKGTAMENNDSFTKL
                     KESTQKLFDAQKKRLNNEDRIETTKNNVIAKHCQTVLSFLVLTSFFVKNCVK"
     gene            complement(10602..10982)
                     /locus_tag="VYN66_001467"
     CDS             complement(10602..10982)
                     /locus_tag="VYN66_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495473.1"
                     /translation="MKLKTLALISGIVGLVGGILLLIGPFVLLGTAVNTVATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDNRVGAAPSVLMIVFGGVSLIPFLGW
                     VGGILVIIGGSLFLATLKKFKSEE"
BASE COUNT         3227 a         2531 c         2055 g         3223 t
ORIGIN      
        1 aatacaagtt tatgatacgt atcgaaaacc tcagtgtctc ctacaaagaa acgttggcac
       61 ttaaggatat ttcactagtg ctccatggac caacaattac cggcatcatt ggtccaaacg
      121 gcgctgggaa atcaacacta ttaaaaggta tgctgggaat tatcccacat caaggtcagg
      181 catttctcga tgacaaggaa gttaaaaaat ccttacaccg aattgcctat gtcgaacaaa
      241 aaatcaatat cgactacaac tttcccatca aggtcaagga atgcgtctcg ttaggactat
      301 ttccctctat tcctctcttt cgaagtttaa aggctaaaca ttggaagaaa gtgcaagagg
      361 cccttgaaat cgtcggccta gctgactacg ctgaacgtca aattagtcaa ctgtctggag
      421 gtcaattcca gcgggtcttg attgccagat gtttggtgca ggaagccgac tatatcctct
      481 tggatgaacc ctttgctggg attgactctg tcagtgagga aatcatcatg aatacgctga
      541 gagatttgaa aaaagctggg aagacggttc tcatcgttca ccacgacctc agcaagattc
      601 cccactactt cgatcaagtc ttacttgtca atcgagaagt gattgccttt ggtccaacaa
      661 aagaaacttt taccgaaacc aatctaaaag aagcttacgg taatcaactc tttttcaatg
      721 gaggtgacct atgattgcag aatttatcga tggattgcaa aaattccatt tcctacaaaa
      781 tgccttgata acagctattg tcgtcgggat tgtagctgga gctgtgggat gtttcattat
      841 tctacgcggg atgtcactca tgggagatgc catttcacat gctgtcttac caggtgtagc
      901 cctctccttc atcttgggcc ttgacttctt tatcggagcc attgtctttg gattgctagc
      961 tgccatcatc attacctaca tcaaaggaaa ctcgattatc aaaagcgata ccgccatcgg
     1021 cattaccttt tcttctttct tagccctcgg tatcatcttg attggtgtcg ctaaaagttc
     1081 aactgacctt ttccatatcc tttttggtaa tatcctggcc gtccaagata cggatatgtt
     1141 tattactatg ggtgtggggg cagccatcct cttgttaatc tggattttct tcaagcaact
     1201 cttgataact tcctttgatg aactcttggc taaagccatg ggaatgcctg tcaatttcta
     1261 tcactacctt ctcatggtac tcctgactct cgtgtctgtg acagccatgc aaagtgtcgg
     1321 aactatcctg attgtagcca tgctgattac cccagctgca actgcttatc tgtatgctaa
     1381 tagtctgaaa agcatgattt tcctttcctc aaccttcgga gctactgctt cagttttggg
     1441 actctttatt ggctatagtt ttaatgttgc ggcaggttct agtatcgtgc ttacagctgc
     1501 tagtttcttt ctcattagct tctttatcgc tcccaaacaa cgatatttga aactgaaaaa
     1561 taaacatttg ttaaaataag gggcaaagcc ctaataaatt ggaggatcta atgaaaaaat
     1621 taggtacatt actcgttctc tttctttctg caatcattct tgtagcatgt gctagcggaa
     1681 aaaaagatac aacttctggt caaaaactaa aagttgttgc tacaaactca atcatcgctg
     1741 atattactaa aaatattgct ggtgacaaaa ttgaccttca tagtatcgtt ccgattgggc
     1801 aagacccaca cgaatacgaa ccacttcctg aagacgttaa gaaaacttct gaggctgatt
     1861 tgattttcta taacggtatc aaccttgaaa caggtggcaa tgcttggttt acaaaattgg
     1921 tagaaaatgc caagaaaact gaaaacaaag actacttcgc agtcagcgac ggcgttgatg
     1981 ttatctacct tgaaggtcaa aatgaaaaag gaaaagaaga cccacacgct tggcttaacc
     2041 ttgaaaacgg tattattttt gctaaaaata tcgccaaaca attgagcgcc aaagacccta
     2101 acaataaaga attctatgaa aaaaatctca aagaatatac tgataagtta gacaaacttg
     2161 ataaagaaag taaggataaa tttaataaga tccctgctga aaagaaactc attgtaacca
     2221 gcgaaggagc attcaaatac ttctctaaag cctatggtgt cccaagtgcc tacatctggg
     2281 aaatcaatac tgaagaagaa ggaactcctg aacaaatcaa gaccttggtt gaaaaacttc
     2341 gccaaacaaa agttccatca ctctttgtag aatcaagtgt ggatgaccgt ccaatgaaaa
     2401 ctgtttctca agacacaaac atcccaatct acgcacaaat ctttactgac tctatcgcag
     2461 aacaaggtaa agaaggcgac agctactaca gcatgatgaa atacaacctt gacaagattg
     2521 ctgaaggatt ggcaaaataa gcctctgaaa aacgtcattc tcatgtgagc tggcgttttt
     2581 tctatgccca catttccggt caaatcattg gaaaattctg actgtttcag atacaatgga
     2641 agaaaaaaga ttggagtatc ctatggtaac ttttctcgga aatcctgtga gctttacagg
     2701 taaacaacta caaggcggcg acaaggcgct tgatttttct cttactacaa cagatctttc
     2761 taaaaaatct ctggctgatt ttgatggcaa gaaaaaagtc ctgagtgtcg ttccttctat
     2821 cgatacaggc atctgctcaa ctcaaacacg tcgttttaat gaagaattag ctggactgga
     2881 caacacggtc gtattgactg tttcaatgga cctacctttt gctcaaaaac gttggtgcgg
     2941 tgctgaaggc cttgacaatg ccattatgct ttcagactac tttgaccatt ctttcgggcg
     3001 cgattatgcc ctcttgatca acgaatggca cctattagca cgcgcagtct ttgtcctcga
     3061 tactgacaat acgattcgct acgttgaata cgtggataat atcaattctg agccaaactt
     3121 cgaagccgca attgcagctg ctaaagccct atagaaaaaa tcctctgtca caaagagttc
     3181 cctgctcttt gaaacggagg atttttgaat attaataaat agaagttcaa tcagctaaaa
     3241 ataactacct tactctacag atttcagggc ttctaacata tcaacctttc tcagataata
     3301 attgacgaag aaaccaagca aggtcaaaat gatgcttact gctgccactg ggattacata
     3361 gacttcccag cctacctgcg gataaaagag aatagtcgca ggcgaaatca tttgaatcaa
     3421 aaattggtgt aaatagaaac cagctaccag accaagtacg attcccacaa gggacagcac
     3481 aatcgtctca cggtaaatgt agagggtgac ttcattatta tgaaaaccga gaaccttgat
     3541 agtggagagt tcacggattc tctcagctac gttgatattg gtcagattgt aaaggatgac
     3601 aatagctaat agaaccgata cgatgaccaa gatggtcatg gtctgattga gtgagctagc
     3661 gatagagtcg aagagtcgaa tggctgaagc attttggaca acgctggaca ccgcagattg
     3721 attcataagc aagcccgcct gactttcgat actagttgca ctggtatccc ttaatgagac
     3781 cagataagtg ttggcttggg gtagctgtcc gtaaagttgc tcatagctag cctgactcat
     3841 ataaataaag tgaccaacgt agttctcagt aatagcagcg acctttagtt ccttaccttc
     3901 aatttctaaa gtctgcccaa ccttgacacc tgccagctgg gcgagtttag ctgtaataac
     3961 gatgccatct tttaatgtca gctcctgctg atgatgttga agatggataa agggagtcaa
     4021 atcttccttc tctatcatca taagagtaat gttttgaaga ccagccttgc ctttgaaatc
     4081 cttgtctagc gttttagaat agattttctg gtaggctagt atctcctgcc ctttcaacac
     4141 ttctgctagc tctaccttgt cctgattggt cgcactagga gtttcagaga caagcatctg
     4201 atactgttgg atttgttgaa actgtttaga cggaactcct gctacagaag attggattcc
     4261 caaacctgca aagagcagag ctacagaacc tgccacacca aagattgtca tcaacattct
     4321 ctgcttataa cgaaagatgt tgcgggctgt taccttatga gtaaaactga gacgacgcca
     4381 gataaaaccg atacgctcca ataagatttt agctcctttg acaggaggtt taggaagtag
     4441 aagctgggct gcttcggcat gaagttccct ccaagccacc agatagactg gtaacacact
     4501 cgccaaccaa ctcaagacaa gagctagtaa gctataggtc caatagaact gaatctgggt
     4561 ttctcccacc actatgcctt ttgtaatgac acttgaaatt acactggcta gcaaataatg
     4621 accaagtata ctacctagag ccgttccgac agtcccagct actagtccat aaaggagaaa
     4681 cttggtgata atatccttac tacggtaacc caaggcctta aaaatccctg catgagttcg
     4741 ctcttcgtct acaaagcgag tcatggtcgt aaaggtcacc atggatgcta cggcataaag
     4801 taccacagga aagatattgc ccactgctcg aatactggat gaagcattgc tatacataag
     4861 atagccctga ccacctggca tggtctgacg attatagacc tgataccttg gctccgccaa
     4921 atcgtccaag acttgctgat gtttttctaa tttttccttt tcttgggcta gattttgttc
     4981 agcttgcttt agtttgtcct cttccttgcc caattcctgc ttggcttggg taagttgagc
     5041 actagcctgc tctctctgaa cttgaggaaa caaggctagt tgactttctt gagcctgtat
     5101 acgagcttga gcagctgcta aacgacgctt gccttcctgc aaattagtct gagccttgtc
     5161 aagggtctct tgccccttgt ctagagactc ttgtccttct tttttcaaaa gttgcagacg
     5221 taccttgcca ttatctgata aagtttgttc aaggtcttcc tgatgttgct tggatttttc
     5281 ttcataagct gatgaaaagg catttaaacc tgctaaatct tgatatttca aacgagctat
     5341 attgtagact ttctgatcaa actgactagg taaaatcacc ccataggctg tcagagtccc
     5401 acttccactt cctgcgtagc ccatatctcg ctgggagagg atttcagccg aatccacaaa
     5461 accagtaatg gtataagtat ggtcttttaa agaggaatga ccctcttctt tttctttaaa
     5521 actaatctcc tgtcccacgc tgtattggcc ttgcaaatga gtggccaaag cgatttcctt
     5581 gtctgactga ggaagtcgtc cctttcttag ctgaaaggtt gaaattcgct ctggtttgga
     5641 gtacagccga atggcatcct gcccattatc catagtcaca tctgtcaaat agccaaactc
     5701 gacctctgcg ccctccgtct gttttagttc ttcttggtct gcttgatcca agccatagtt
     5761 agacatgact gccaaatcca aggtttgagc agttgttaaa taagcattag ctgtcgcctc
     5821 catgttggga ctggttactt tgaggcctac taaggctaga gatcccaaca tcatcaggat
     5881 caagatggat aaaaaacgcc ccttggagcc tgtgaaggac tgaattaagt ccttccaata
     5941 agtttttcgc ttgatcatgc tagtactcca aactgtcaat atcctgagga tgctggttga
     6001 gcaccacatc cttgacactg gcatcgtgca tttgaatcac gcgatcagca atgggcgcca
     6061 aagctccatt atgagtcacg atgatcaccg tcgctccctt ttgacgagac atgtcttgga
     6121 gaattttcaa aacctgcttg cccgtctgat aatccaaggc tccagtcggt tcatcacaaa
     6181 ggagaatttt aggatttttg gctaccgcgc gtgcaatgga gactcgctgt tgctcccctc
     6241 cagaaagctg ggctggaaag ttattgagac gatgagccag acctacatct gtcaagacct
     6301 gatcaggatt caaggcatct gtcacaattt cagaagccag ttccacattt tccttagctg
     6361 tcagattaga aactagatta taaaactgaa aaacaaaccc cacatcattt ctacggtaat
     6421 tggtgcgctg gtgggaacta taatccgcaa tattaacacc atcaatccag atttcccctt
     6481 catcattggt atccattccc ccaagaaggt taagaactgt tgacttgcct gcacctgaag
     6541 caccaaggat aataaccagc tccccctttt caatctcaaa attcacatca caattggcca
     6601 caatctccgt gtccccaacc tgataacgct tgtaacagtg tttcatctca atataagcca
     6661 tcagacccac cctctatcaa acaaattata ctcctcgaaa atctctcttc aaaccacgtc
     6721 agcttccatc tgcaacctca aaacactgtt ttgagcaacc tgcggctagc ttcctagttt
     6781 gtgctctttg attttcattg agtattactt cctattatca ttataacgtt ttttcaacta
     6841 gttgaaaact gcttatattc ttaaatcctt attaaaaggc agtttcaaat tactgcctta
     6901 taacatcttc ttaaaaataa aattgaacaa gatcaccttc tgctgttgcc accgaaatac
     6961 aatctttccc catgcatttc gcttttattt tttcttgatt cattttaatc acactcttct
     7021 caatatctgg ataagaaact cgcaaatctc ctccactcct tgataaaatg gtcaactgta
     7081 agagtttttt atcttcccag ctcatgctca cttcaaaatg tccacgtgcc attaagcctg
     7141 aaacagaacc tgttgaccaa gcatcaggta gggcagctag aggtaccaga taagctgcat
     7201 gagactggag taacatttct gccatgccac tagtagcacc aaaattacca tctatctgaa
     7261 aaggaggatg gctacaccaa agatttggca aggtggatgt ctttaactgc tctgccaata
     7321 atttatgggc tcgattgcca tctcccaaac gcgcccagag attgatctta ttagccttgg
     7381 accagcctgt gccgccatct ccacgatcat tgaggctagc acgtaccgct tcaatatact
     7441 cttgtccctt gtagctaaag agattgccag gatagagtcc cactagatgg gaagcgtgcc
     7501 gatgctgggc ctccactttc tcattttgaa aatactgctc ttcctcctca taccactccc
     7561 tgattcgacc agattgagtg atttgcaaag gatttagtaa atcagacttc tccttaacct
     7621 cagtcaacaa gtcctcatcc agtcccaatt cctgagcagc ctgaataaaa tcatgaaata
     7681 actgccaaat cagagattgg tcataagtat tgccaatcga aatcggccca tgttctgggg
     7741 aataagacgg agaagacacc caacgctgcg cctgctgatc cttatgtaaa aaggcattcc
     7801 aaaaacgaac cgtttccctc aacatgggat aaattttctc cctgagatag tcttggtccc
     7861 tataaaatga ataggcttca taaacggttt gcatcatcca cgcattggca gctggtgacc
     7921 aaccccaata gtaatcccaa ccaggtgccg tccaaccaaa gggagtcgct tgagtatgaa
     7981 ccaaccaacc attctcctca cctttctgag agacgattcc tgcatacttt acagccgcta
     8041 gacgaccata gacacgcaaa tcatctacat agttgatgac tggaaagacc gtctctagga
     8101 gattggtaac ataggctggc caataattca tctgcagatt gacatttaag tgatagtccg
     8161 aattccaagg aggattgtcg accgcattcc agactccctg taggttagct ggtagagcat
     8221 ctgggcagtc tctggacgaa ctaatcaata aataccgtcc atactggaag aacagctcct
     8281 ccaaagcctg cccttcttgt ggcttataat tttttaacaa atcatctgta gtgaatgcgt
     8341 caacattagc ttccaaatcc aattgaacac gctggaataa ggcttggtag tcctcgatat
     8401 gccttgattt caattgggta tagccctttt ctttagctgt gtccaccaag tctatcacct
     8461 gttgctctaa atctagtttc ttgcgataat tgctagcagg attttgggca aaatccgtct
     8521 tagcggccaa gaagagattg gcataactgg ctcctgatat ctgaacccta tctgaccaaa
     8581 ctctaatatc tccatccgtt tcccaagcta gataactagc aaaccgcaga tcattatcct
     8641 taactcttcc cttcatcaag atatgagaat cagtaatatc caacttacac tccttgtaat
     8701 cagatttttc ctgctcatac tttccatcag aagccaaatc acaggtcaag gatagttcta
     8761 tagtaaaatc tagagtttcc gccccttcct tagtaaaacg ctgaaccaag agatcatctg
     8821 gaaaactcgc aaaagcttca cgttcaaatc gcgttccctt atagacataa gaagtcgtca
     8881 caagtgcctt actaatattc agctgtctct gatagtccgt cacctgagac aaagtcgtac
     8941 cttgctggct gaactcaatg tgaatatccc caaaagacag ataggtccca tattgactcg
     9001 tttttggccc aattaggtgc tgctcagcca gttccttagc cagattgtaa tctctcttct
     9061 ccaaagcctg ccgaatctca gctaaaaaaa catactgatc ctgaagattt ccaccctgat
     9121 aatctgaact atcaggaagt ggacctccag accagagact tttttcatta aattgaatcc
     9181 gttcagcccc tataaggcca aatacttttg ctcctaaaga accattgcct atcggtaaag
     9241 cttcttcttc ccaacccatg taagtggttg aaactggttg cttgtaggcc agtacataat
     9301 cttgtttttt attccttatc atacttccat actaacataa aaagcctaga aatcaattct
     9361 agacttacaa ttttttattt tccaaggtag tattcagaaa cgacgttcaa tttttcgtcg
     9421 aattcgaata ccaatggtgg gaagttaggg atttccacgt ccatgatctc gtcatctgac
     9481 aaacctttga tgtgttttac aagggcacgg attgagttac cgtgagctcc tacgaatacg
     9541 tttttaccat ctttaagagc tggagcgatt ttatcttccc agaatggaag agcacgttcc
     9601 aaagtcactt tcaagttttc agcatctggg attactgagt cgtcaagtga agcataacga
     9661 cggtctgtgt gagctgagtg ctcatcatca cggtccatat ttggaggcaa tacatcgtat
     9721 gaacgacgcc agatgtgaac ttgctcatca ccaaattgtt cagcagcttc agctttattt
     9781 ttaccagtca aaccaccgta gtgacgttcg ttcaagcgcc atgatttttc aactggaacc
     9841 cacaattggt cagaagcttc aagagccaag ttagttgttt tgatagcacg tttcaatact
     9901 gaagtgtaag cttggtcaaa tttgatacca gcttctttga tcaatttacc agcgtcaatc
     9961 gcttgttgtg tacctttttc agacaaatca acatcagccc aaccagtgaa aaggttagct
    10021 ttgttccatt cagactcacc gtggcgagca aaaaccaatt ttaccattag atggattctc
    10081 ctttaaattt tccgaggttt cccctcgttt atctattcta ttttacacaa tttttcacaa
    10141 aaaagctagt caaaaccaaa aaggaaagga ctgtttgaca atgtttggca atcacattgt
    10201 ttttagttgt ttctattcta tcttcattat ttaacctttt tttctgcgca tcgaataatt
    10261 tttgagttga ctcttttagt ttagtaaacg agtcattatt ttccatagct gtgcctttga
    10321 aaccttcctc agctttagta aaataatcat caaaaatatt tttataaaga tctatataat
    10381 ctgcataagt gcgtattgat tcaatcacat cgtctgttac aaccggtgta tcgaaagtga
    10441 ttgtttcttt tgaactattg gatgttgcta ctgtcttctc atcattactt gttgaagtat
    10501 cctttgaact ttatgttcca catgcaacta gaagaaaaag agataataga gctactccta
    10561 taccgataaa cttttttgtt cttttcatgc taaaatacct tttattcttc tgatttgaat
    10621 ttcttcaatg ttgcaaggaa tagagatcct ccgataataa caagaatccc cccaacccat
    10681 cctaagaatg gaatgagact aactccacca aaaacaatca ttagtacaga cggagctgca
    10741 cctacacgat tatctccttt atagtaaaca atagcaatga taccaagaac aagatttgca
    10801 atcttcaagg cattcaagag taaggcaaca cctgaaaaag cccctgcagt agctcctcca
    10861 ttaagagttg tagcaactgt gtttaccgct gttcccaaca agacaaaagg accaataaga
    10921 agtaaaattc ctcccacaag accgacgata ccactaatta aagcaagtgt ttttaatttc
    10981 ataataaaat ctcctttttt atttttttca aataaaattg tatcatattt gctttt
//
LOCUS       DAVLVF010000059        10915 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVLVF010000059 DAVLVF010000000
VERSION     DAVLVF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.059"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(319..1125)
                     /gene="yidA"
                     /locus_tag="VYN66_001468"
     CDS             complement(319..1125)
                     /gene="yidA"
                     /locus_tag="VYN66_001468"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV1495474.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(1138..2442)
                     /locus_tag="VYN66_001469"
     CDS             complement(1138..2442)
                     /locus_tag="VYN66_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1495475.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2514..2891
                     /locus_tag="VYN66_001470"
     CDS             2514..2891
                     /locus_tag="VYN66_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV1495476.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTNRYQVDGQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            2982..3314
                     /locus_tag="VYN66_001471"
     CDS             2982..3314
                     /locus_tag="VYN66_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV1495477.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            3326..4897
                     /gene="ffh"
                     /locus_tag="VYN66_001472"
     CDS             3326..4897
                     /gene="ffh"
                     /locus_tag="VYN66_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV1495478.1"
                     /translation="MAFESLTERLQNVFKSLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(5092..6375)
                     /locus_tag="VYN66_001473"
     CDS             complement(5092..6375)
                     /locus_tag="VYN66_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV1495479.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6678..7391)
                     /gene="rsmG"
                     /locus_tag="VYN66_001474"
     CDS             complement(6678..7391)
                     /gene="rsmG"
                     /locus_tag="VYN66_001474"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV1495480.1"
                     /translation="MKPETFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7485..8045
                     /locus_tag="VYN66_001475"
     CDS             7485..8045
                     /locus_tag="VYN66_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV1495481.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            8047..8946
                     /gene="htpX"
                     /locus_tag="VYN66_001476"
     CDS             8047..8946
                     /gene="htpX"
                     /locus_tag="VYN66_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV1495482.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKHM"
     gene            complement(9095..10654)
                     /locus_tag="VYN66_001477"
     CDS             complement(9095..10654)
                     /locus_tag="VYN66_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1495483.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
BASE COUNT         3188 a         2022 c         2439 g         3266 t
ORIGIN      
        1 tactactgta agatatgata aagtcttatt tttagtgaag tatcctaatt tgtataattg
       61 attgtgtgta gattcagggt caatttgaag agagtattta aaatatcttg ggaaattata
      121 atttctccct gttttttgag tccaatctat gagtatgagt tgcccaggag taaggccaca
      181 ttctaaatcg tttaggtaaa aattgttaca atcagaattt gtgtctctat tatgattatt
      241 ttgttttaaa ttaaatagtt tgctaaaaaa attcattgga ttttattgct aatcaattct
      301 tattgaaaaa tgatactttt acagtaccca tgttcggatg gcgtgggcaa cgccggattc
      361 gtcatttgtt ttagtgatgt atttggcgat ttttttgatt tctggatttc cattttccat
      421 gacaacgggg tttccaacga cttccagcat ggcacggtca ttttcttcgt caccgattgc
      481 catggtttca tctttggtca atccgagttt ttcagccaag tgagtaatag ctgaaccctt
      541 gtctacattc tttttaagga gttcgaggta gaaaggagca gatttgttga tagagtagtg
      601 ctcgtaaaat tcggctggta ttttttcaat cgcagcatcg agaatttctg gttcatcgat
      661 gaacatacac ttgacaattt ccttgccagc catttcttca ggggtacggt agaagatagg
      721 catgctgacg agggttgatt cgtgtacagt gtatgttccg atattgcgat ttgcagtata
      781 gataccgtcc ttggtaatgg catgcatgtg gacaccgagc ttgcgactga ggaattccat
      841 atccagataa tcctcatagg tcaaggattc gctgataatc tcatggccag tagcagtttc
      901 ttggacaagg gcaccattga aggtcaccac atagtcacct tggtctctca actgcaagtc
      961 gtccagaagt ttggcaacac ctgcgatggg gcggccagtt gcaatcacga ctttgacacc
     1021 agcttctttg gcatcttgga tggcagaaaa gacttcagga gtgatttcct tttggctgtt
     1081 gactagggtt ccgtcaatat cgacggcgat tagtttaata ctcataaatt tcctctttta
     1141 gtttttattt ggggtaaaat gatcgttctc aatcaagtgt aaaaattgct gggtgatgct
     1201 tgcgaagatg ctgttttggt ctaacatttc tttcgggaaa taaaagcgat tatctccgtg
     1261 gcgacttcca gcaagggatt ggacgatagg ggacaggcta gagagttctg ccagttctcc
     1321 atttttttgt aaaatctcaa tctgtgtcct tggattttca gattcgggac ggtaaatatc
     1381 ataagggagg tcaaagttct tatgaatggc agtatagtag tcgggatcaa agccgatgtc
     1441 ctcaaccaat tttctcatgc tagcgagttg gtcttggtct tcttgtgaaa aggtaatgga
     1501 tttaaagacc ttgcggttga caaagcgatg cgataaatct gcaagaatct tgtcaggact
     1561 ggtcatccaa agctggaagt aggtattcat cacgccatca tccagagcca gatagtcagt
     1621 caaggtcaca tttttttcga agaaaggcag gaggtgtgga gaagttcggg caaagaaatc
     1681 cttgtcctca ggatagagtt ccttggcgcg tttgagaaga ttctgtagga gaacttccat
     1741 ggcgcgtgtt gcggggtgga aataaacctg catgtacatc tggtagcgac tgaggacgta
     1801 gtcttcgatg gcgtgcatgc cattgcgctg aaaggcgata ccattttcga taggacgaat
     1861 gactcggagg attcgagtca ggtcaaattc cccataggat gctcctgtaa aataggagtc
     1921 gcgcaagaga tagtccatgc gatctgcgtc aatctgacta gaaatgagct gcacgacctg
     1981 cttattagga taggtatggt caatgacact ggccaccttt tctgggaaat caggtgccac
     2041 ttgtagcagg acttggtgaa tctctgtctc aggattttga ataatctcct gagtaatggc
     2101 ttcatggtct gtatcaaaga gattttcaaa agtatgggag taggcaccat gcccaaggtc
     2161 gtgtaggaga gcagcggtca tggtcaagag agactcggca ggattccatt cctcaggata
     2221 tttttcttcg aaaatctctg tgatgcgtcg tgcaatttca tagactccta gacagtgaga
     2281 gaagcgactg tgttctccac cgtggaaggt ataactggaa gttcccagtt gtttgatccg
     2341 gcgcaaacgc tgaaattctt ttgtattaat caagtcatag atgatttgat tattgacatg
     2401 gatgtagttg tgaacagggt cacggaatac tttttcgttc atatgaccat tatagcaaaa
     2461 tcctgctctt tttggtaaaa tagtaggaca agagacaaga aagagggcct gatgtgaaga
     2521 ttcggatgcg aaatacaatt cagtttgatg agcagttgga agtgattgac cagctttatg
     2581 atgtggaagt gcatgaaaaa ggagattata gctacctgct tttctataat gaggaaaagg
     2641 aaaaagtagt gattaaattt catggtcaag aacttgtgat gagtcgattt tccaatccca
     2701 agaccattat gcgctttcta aaggatagtg atagtttagc ctatattccc acacctatgg
     2761 gtatgcagga gtttattatc caaacgaatc gttatcaagt ggatgggcaa aagattgaac
     2821 tagattatca actacaaaat caagagggac atccctttgc cagctatcaa ttggaaatta
     2881 cttggggcta ggcttatgtc tttttcaaat ttagctatct atcttcttgg atcggcttcc
     2941 tagtgtggta gattaggcta atttttggta taataaaagt tatggaaatc gaaaaaacca
     3001 atcgtatgaa tgcgctcttt gaattttatg cggcgctttt gacagataag caaatgaatt
     3061 atatcgagct ctactacgct gatgattaca gccttgctga aattgccgag gagttcggtg
     3121 tcagtcgtca ggctgtctat gacaatatca agcgaacaga aaagattctg gaagattatg
     3181 agatgaaatt gcacatgtac tcggactata ttgtccgcag tcagattttt gatcagattt
     3241 tggagcgcta tcccaaggat aactttctgc aggagcagat agaaatttta acaagcattg
     3301 ataatagaga ataagaggaa gaaaaatggc atttgaaagt ttaacagaac gtttgcagaa
     3361 cgtctttaaa agtctacgta aaaaaggaaa aatctctgaa tctgatgtcc aagaggcaac
     3421 caaagaaatt cgcttggcct tgcttgaggc cgacgttgct ttgcctgttg taaaggactt
     3481 tatcaagaaa gttcgtgagc gtgcagtcgg gcatgaggtc attgatacac ttaatcctgc
     3541 gcaacagatt attaaaatcg ttgatgagga actgacagcc gttttaggtt ctgatacggc
     3601 agaaattatc aagtcaccta agattccaac catcatcatg atggttggtt tacaaggggc
     3661 tggtaaaaca acctttgctg gtaaattggc caacaaactc aagaaagaag aaaatgctcg
     3721 tcctttgatg attgcggcgg atatttatcg tccagctgcc attgaccagc ttaagacttt
     3781 gggacaacag attgatgtgc ctgtctttgc acttggaaca gaagtaccag ctgttgagat
     3841 tgtacgtcaa ggtttggagc aagcccaaac taatcataac gactatgtct tgattgatac
     3901 tgcgggtcgt ttgcaaattg atgagctcct catgaatgag cttcgtgatg tgaaagcatt
     3961 ggctcaacca aatgaaattc tcctcgtcgt tgatgccatg attggtcagg aagcagccaa
     4021 tgttgcgcgt gagtttaatg ctcagttgga agtgactggg gtcatcctta ccaagattga
     4081 tggcgatact cgtggtggtg cggctctatc ggttcgtcac atcactggaa aaccaatcaa
     4141 gttcactggt acaggtgaaa aaattacaga tatcgaaacc ttccacccag accgtatgtc
     4201 tagccgtatc cttggcatgg gggatatgct cactttgatt gagaaagctt ctcaggaata
     4261 cgatgaacaa aaagcccttg aaatggctga gaagatgcgc gaaaacacct ttgattttaa
     4321 tgatttcatc gatcaattag atcaggtgca aaatatgggg ccgatggaag acttgctcaa
     4381 gatgattcca ggtatggcca acaatccagc acttcaaaac atgaaggtgg atgaacgcca
     4441 gattgctcgt aaacgtgcca ttgtgtcttc gatgacacct gaagaacgtg aaaacccaga
     4501 tttgttaaat ccaagccgtc gccgtcgtat tgctgctggt tctggaaata cattcgtcga
     4561 agtcaataaa tttatcaagg actttaacca ggctaaacag ctcatgcagg gtgttatgtc
     4621 tggggatatg aataaaatga tgaagcaaat ggggattaat ccaaataacc ttcctaaaaa
     4681 tatgccaaat atgggaggaa tggatatgtc tgcccttgaa ggaatgatgg gacaaggcgg
     4741 tatgcctgac ttatcagctc tcggaggagc aggaatgcca gatatgagcc agatgtttgg
     4801 tggcggtttg aaaggtaaaa ttggtgaatt tgccatgaaa cagtccatga aacgtatggc
     4861 taacaaaatg aagaaagcga agaagaaacg caagtaagac aagagattga tgcaaatctc
     4921 atttgatgag aaaataaagt aaaaatttct atgataaaac gtacagtatc aaggttttag
     4981 aatatctgat attatacgtt ttttgatttt aataattttg ttaagacact tttattatga
     5041 tatacgaaaa atccactcag ctgtctgagt ggattaggtg tatttagact cttagtcttt
     5101 attttcgtat ggcaagatca agttcaagat gattcctgtc atggctgaaa gggcagtacc
     5161 tgaaagtgta actggaccaa gtttaaggat agctcctcca agtccaagaa ccaacatagc
     5221 acttgcgatg atgaggtttc gcatttgagc gaaatcaaca cgttctttaa tcaagacttt
     5281 caaaccattg ctggcgataa ccccatagag aaggattgac ataccaccaa gtacagcgtt
     5341 tggaatagtt gaaatcaagg cagtgaattt accaaggaag ctgagggcaa tcgcgatgaa
     5401 ggcagcgtta cggataactg agacagaagc gatacgagtc ataccgataa cccctgtatt
     5461 ttctccgtaa gttgtattgg ctggtccacc aaggaaggca gaaacagaag ttgcgatacc
     5521 gtcaccaata agagtacggt gaagacctgg ttcttttaag aattgacggc cacagatttg
     5581 acccaaaaca gtatggtctc cgatatgttc agaaattgtt acgatagcga ttggcaagat
     5641 agcgatggct tctggaccaa agtaaagatt gtactcttta aaggcaccac ctgtgctaaa
     5701 tggcaagtag aaaccaggaa tttcgaacca gttggcttta agaactggtg taaagtcaac
     5761 caagccaaga gttagtgcga aaaggtaacc accgataatg gcaaagagga atggaatgat
     5821 tcgtaggaag ccttttcctt ttgtattgat aaaggcagca attaggaaag taacaacggc
     5881 taccagagca tttttccaat ttccgtctgc tacaagacct gcattggtaa cagctgaacc
     5941 tgcaagtcca agaccgataa cgatgatcat aggaccgata atgattggtg gcaagagttt
     6001 atcaatccat tttgttccta caaatcggat gctggtagca acaaggacat agaccaaacc
     6061 agtcaagata acccctgttt gggcagcaga tacatccccc cccatttctt tcatagccag
     6121 tgacatagct gtgataaagg caaatgaaga acctagataa actggaactt taaaaccagt
     6181 agcaatcatg tagatgagtg ttccaacacc tgaagcaaaa agggcaacag atacaggcat
     6241 tcccaaaatc aatggtacca agatggtcgc accaaacatg gcgaaaacgt gttggaagct
     6301 aaggagaatt ccttttccat ccgaaggacg ttggtcaacg tctagtaaca aatcaacagt
     6361 tgattcttgt ttcatataaa cctcactttt gggcatcaga aaagagccca ttattttaca
     6421 gcaataggcc ctcagagtaa gacttctcta aaatctagac tttaaagagt ttcaaatatt
     6481 tctgcgtctt cgtcacctca cgggatgaca ttaaaaacct ttgcttagga tagtatagca
     6541 gaaaaaaatg attttgtaaa tcattttttc ccgagcctag aaataaaaga gcgaggctga
     6601 ttttgtaaat cattttttcc cgagcctaga aataaaagag cgaggctgat tttgtaaaca
     6661 tttgtttact aaaaaattta aagtgggcgt ttatttggca taccagcctt acgtggatat
     6721 ttatttggtg tttctttttt cttttctacc actgtgatat agcgcggatc tctattcggt
     6781 agggcgtagc tgagattgtc ttcgacctta ctaaaaagga gattgagggc attcttagct
     6841 tctaacaact cctcaggcgc attgctagcc ttgagtgcta atagtttgcc accaacctta
     6901 aggtagggaa tagtcaattc agataggacc tgcatacggg caaccgcacg agctgttaca
     6961 aaatcatatt gagcacggaa gttcttgtct tgggcaaaat cttcggcacg tccgtggtag
     7021 aaatgaactc cgttcaaatc cagttcttga gccaagagtt gtaggaagtt gatgcgctta
     7081 ttgagtgaat caataatggt cacatctaac tccggataga gaattttcat tggtagacta
     7141 ggaaatcctg ccccagcccc gatatcaaga agtttgatag tttcattggg aatcaaacct
     7201 tgaagaatgg gtgcaatcga atcgtaaaaa tgtttgagat aaacttcttc cttgtccgta
     7261 atcgccgtca aattaatctt ctcattccac tcgaccaaga gctcaaaata acgttcaaat
     7321 tgttcttttt gctggtccga aagtggaaga ttctgctcgg caagcaagtt gtaaaatgtt
     7381 tctggtttca tagttatttc cttctttagt atcatcttat tttaccacaa tcattaaaaa
     7441 tttgatatac tggtactaat ctaaaagtag aaaggactta tatcatgact tggattattc
     7501 ttggagttat cgctcttatt gttatttttg tgattgttag ctataacggt ttggttaaaa
     7561 atcgtatgca aaccaaggag gcttggagtc agattgatgt tcagttgaaa cgtcgaaatg
     7621 acctcttgcc aaacttgatt gagactgtaa aaggttatgc caaatatgaa ggttctaccc
     7681 ttgaaaaggt ggcagaacta cgtaaccaag tggcggcagc gacttcacca gcagaagcta
     7741 tgaaagccag tgatgccctc actcgtcaag tttcaggtat ttttgcagtt gcagaaagct
     7801 atccagattt gaaagctagt gctaactttg ttaaattgca agaggagttg acaaacacag
     7861 aaaataaaat ttcttactct cgtcaactct ataacagtgt tgtcagcaac tacaatgtaa
     7921 aattagaaac tttcccgagc aatattatcg ctggaatgtt tggatttaaa gcggcagatt
     7981 tccttcaaac acctgaagag gaaaagtcgg ttcctaaagt tgattttagc ggtttaggtg
     8041 actaagatgt tgtttgatca aattgcaagc aataaacgaa aaacctggat tttgttgctg
     8101 gtatttttcc tactcttagc tcttgttggt tatgcggttg gttatctctt tataagatct
     8161 ggacttggtg gtttggttat cgcactgatt atcggcttta tctacgcttt gtctatgatt
     8221 tttcaatcga cagagattgt catgtccatg aatggagcgc gtgaggtgga tgagcaaacg
     8281 gcaccagacc tctaccatgt agtggaagat atggctctgg tcgctcagat tcctatgccc
     8341 cgtgttttca tcattgatga tccagcctta aatgcctttg cgacaggttc taatcctcaa
     8401 aatgcggctg ttgctgcgac ttcaggtcta ctagctatca tgaatcgtga agaactagaa
     8461 gctgttatgg gacatgaagt cagtcatatt cgtaattatg atatccgtat ttcgactatt
     8521 gcagttgccc ttgctagtgc tatcaccatg ctttctagta tggcaggtcg tatgatgtgg
     8581 tggggtggag caggtcgcag acgaagtgat gatgaccgag atggaaatgg tcttgaaatc
     8641 attatgctag tggtttccct actagctatt gtactggcac ctctcgctgc aaccttggtt
     8701 cagctcgcta tttctcgtca gagggaattt ctggcagatg catctagtgt cgagctgact
     8761 cgcaatcccc agggaatgat taatgcccta gataagttgg acaatagcaa acctatgagt
     8821 cgccacgtcg atgatgctag cagtgccctt tatatcaatg atcccaagaa aggtgggggg
     8881 ttccaaaaac tcttttatac ccacccacct atctcagaac ggattgaacg tttaaaacat
     8941 atgtaaaatg aaggctggaa aaaagtcttt aaaatctgaa aaacgcataa tatcaggtgt
     9001 gaaaacttga tattatgcgt tttactatgg gaagatttac ttctttttct cctaaaattg
     9061 tgtttttgcc ccacctatct gctatgttgc aaatttataa atcttctaaa ttaactagct
     9121 tcaaaccgtg ttctgtcatg cgatagatga tcgagcagac ttcttttaag aaacgacggt
     9181 catgcgaaac agtgatgaga ccgccaggat aagtggcaaa gagttttctg atttggggtt
     9241 gagaagtggg agaaaagttt cgtgtgggtt catccagcag gagaaagttt ggtttgcgca
     9301 ggactaaatc caaaagcagg agttttccct gttgtccgcc agataaggag cgaatttgat
     9361 gctgcatttc tggataactg aaattgagac tagctaggtg agattggatt ttctgtagtt
     9421 cctctttttc cccagtttta ctgagatagg ctattgggga taaatccaat tgcagttttt
     9481 tgtggtaatc ttgtggcata aaaccaagtg aaatctctct tttatcattc agaagtctct
     9541 gtaacttggc taacagagtt gatttcccaa caccatttgg cccgataata ccgatttttt
     9601 cttggccacg gacagttagt tgtagttttt gaaccaaaac tcggtcgtca atggacaaat
     9661 tttctttttc cagttggact aagactttag aagctggtaa tggttggatg tctgaaaaga
     9721 aaagttggat ttgttcctct tcaagtggtt tttgagtcat ggactgagct gccttttcgt
     9781 agcgtttttc ttgtgagagg acagttttca tctttttagc caataggcga ccggcagtac
     9841 tatctttggt agctcgaagc gcagtttcta cattttgctt aactctccga tgtttttcca
     9901 tggttttatc gtaggctctt tggttgttag cagcttgctg actttgtttg gcaaaattag
     9961 cctttctctg ctcactatag ctatcatagt ctaaatgctc tactagcgtt tccgcttctt
    10021 tacggtgttt gaccagtcgc aagtgaacaa tagtgtctgc cgtttcagaa agaaagtctt
    10081 catcatggga aatgaaaata acggtttgcc tggtcttttg aatctggcct tttagccaat
    10141 caactgtctc aaggtctagg tcatttgaag gttcatctaa aaatagaatc tcaaagggtt
    10201 tggctaactc atggataagc tgaattttca aagcttctcc ccctgataga ttgccaatct
    10261 cttggtcact tgcgaaacga ttgctatcaa aatgcaattc ctccgccaaa cgatagagga
    10321 tactgtagtc taaatcaata gaatctaaaa agaagtagtc gtgtaaagtt ttctttttca
    10381 gctcctcggg aagtttttga ggaatgtagg ccagtgactg atagtcagat tggatgtctc
    10441 ccttgatagt gaaatcagac aaatcttccc ccattaaagt tttaagtaag gttgatttac
    10501 catttccttc ttcgccaata atagctacct tttccccgtc ttgtatggtc atgcttaggt
    10561 cagagacaag gtctcgtaaa tctttgtttt gtgtgatggt caggtgattg atttttatca
    10621 tactattgcc ctttctagaa tgtcgatagt gcataacaaa aagctctact ttacctagta
    10681 gagcccaaag tctatgtcaa aaaaactcta ttaacatgcc agaaaaagag cataaaagag
    10741 cacacaaaaa gagacaaaaa caagatctac taaataaagg ttctttattt gatagactta
    10801 gttgttcatg tctcccttac ctccgagtat tagtatcttt atattatacc tttaaggaaa
    10861 ttttgtcaac agaaaactgg aaattctatg ctctaaaatc caaaaacagg tccat
//
LOCUS       DAVLVF010000060        10840 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVLVF010000060 DAVLVF010000000
VERSION     DAVLVF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10840
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.060"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            117..281
                     /locus_tag="VYN66_001478"
     CDS             117..281
                     /locus_tag="VYN66_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1495484.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            365..931
                     /gene="amaP"
                     /locus_tag="VYN66_001479"
     CDS             365..931
                     /gene="amaP"
                     /locus_tag="VYN66_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV1495485.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            943..1113
                     /locus_tag="VYN66_001480"
     CDS             943..1113
                     /locus_tag="VYN66_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV1495486.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1152..1760
                     /locus_tag="VYN66_001481"
     CDS             1152..1760
                     /locus_tag="VYN66_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1495487.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1791..1994
                     /locus_tag="VYN66_001482"
     CDS             1791..1994
                     /locus_tag="VYN66_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1495488.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2104..2382)
                     /locus_tag="VYN66_001483"
                     /pseudo
     CDS             complement(2104..2382)
                     /locus_tag="VYN66_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3146..3916)
                     /locus_tag="VYN66_001484"
     CDS             complement(3146..3916)
                     /locus_tag="VYN66_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV1495489.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTTKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3931..4779)
                     /locus_tag="VYN66_001485"
     CDS             complement(3931..4779)
                     /locus_tag="VYN66_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV1495490.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDNFEDPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTGLDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4776..6251)
                     /locus_tag="VYN66_001486"
     CDS             complement(4776..6251)
                     /locus_tag="VYN66_001486"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV1495491.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVNFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6574..7929)
                     /locus_tag="VYN66_001487"
     CDS             complement(6574..7929)
                     /locus_tag="VYN66_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1495492.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLNQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWHAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(7939..8256)
                     /locus_tag="VYN66_001488"
     CDS             complement(7939..8256)
                     /locus_tag="VYN66_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1495493.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8273..8590)
                     /locus_tag="VYN66_001489"
     CDS             complement(8273..8590)
                     /locus_tag="VYN66_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1495494.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8605..9501)
                     /locus_tag="VYN66_001490"
     CDS             complement(8605..9501)
                     /locus_tag="VYN66_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV1495495.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9504..10766)
                     /locus_tag="VYN66_001491"
     CDS             complement(9504..10766)
                     /locus_tag="VYN66_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1495496.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
BASE COUNT         3720 a         2183 c         1681 g         3256 t
ORIGIN      
        1 aagaaattga ttttttgata ttaaaataaa aaaggagggt aggcagtgtt gtgatcaatt
       61 attgctggag gtcttattgg tctcttggca ggtaaaatca ctaaaaaagt agttctatgg
      121 gaatcatcgc aaatgtattc gctggtttag tcggggcata tgcaggacaa tctcttttag
      181 gtagttgggg tccatcaatc gctggaatgg ctttgctccc atctattgta ggtgcagcga
      241 ttgtgattac tgtagtgtca ttctttacag gtagaaagta aacttttcgc cagtaaagtt
      301 agcaaactat ttttaaatca atgacgggaa aaatagttta aatgttaaat cgaaaggatt
      361 gtatatgtca aaagcaaaga aaatatgttt cattattttc tgtattttaa tcttgacaat
      421 tttccttcct gttttgatag attatcatca agttagtgat ctaggtattc atctacttag
      481 ctggagacag aactccgtag ttgaattcta tcttgctaga tatgtctttt gggggacagt
      541 ggttctatca actttagttt tattatccat tttagttgtg atgttttatc ctaaacgtta
      601 cttggaaatc caacttgaaa ctaaaaacga tacattaaaa ttaaagaatt cggcaatcga
      661 aggttttgtt agaagtttgg tgagtgatca tagattgatc aagaacccaa ctgttcatgt
      721 aaatttacga aaaaataaat gtttcgttca tgtagaaggt aaaattcttc cttcagacaa
      781 catcgctgac agatgccaaa taattcaaaa tgaaataact aatggattga agcagttttt
      841 tggtattgag cgtcaagtaa aacttgaagt tgcagtaaaa aattaccaac caaaacctca
      901 aaacaaaaag actgttagtc gtgtgaagta aggaagtaaa aaatggaatg gcttaaacaa
      961 tatcgatatc caattatcgc tggtctcata ggcgtatttc tggcttgttt gattgtctcc
     1021 tttggcttct tcaaaacaat atttgtattg attttaggag cactgggagt tgcagctgga
     1081 ttatatatcg aaaaaaacta tatagataaa taaaaaaata aaaattacta atttaattaa
     1141 aggagtttca tatgtcaaac gaaaaaaaca caaacactaa cgtagaaaag aaagatgcta
     1201 ctgttgtagc tcacgaaatc aaaggggaac ttacttacga agataaagtt atccaaaaaa
     1261 tcattggtct ttcactagaa aacgtttcag gtcttttggg aatcgatggt ggtttcttct
     1321 caaatcttaa agaaaaaatc gttaacagcg atgacgtaac aagtggtgtt aacgtagaag
     1381 ttggtaaaac acaagttgca gttgacttaa acgttattgt tgagtaccaa aaaaatgttc
     1441 cagctttata ttcagaaatc agagaaatcg tatcttcaga agttgctaaa atgactgact
     1501 tggaaattgt tgaaatcaac gtaaacgttg tcgacatcaa aactaaagaa cagcatgaag
     1561 cagactcagt aagccttcaa gatcgcgtat ctgacgttgc tgaatcaaca ggagaattca
     1621 cttcagaaca attcgaaaaa gctaaatctg gtcttggatc tggtttctca actgttcaag
     1681 aaaaagttag cgaaggtgta gaagctgtta aaggtgcagc aaatggtgta gtatctcacg
     1741 aaaacactcg tgtaaactaa gataaaataa atataacagg agaaattatc atgtcagtag
     1801 aagaaaaatt aaatcaagct aaaggttcta ttaaagaagg tgttgggaaa gccatcggtg
     1861 atgaaaaaat ggaaaaagaa ggagcagctg aaaaagttgt ttctaaagta aaagaagtcg
     1921 ccgaagatgc caaagacgct gtagaaggtg ctgtagaagg tgttaaaaac atgttgagtg
     1981 gcgacgataa ataaggttaa aagttacttt atctttttag taacattagt caaaagagtc
     2041 tgagtcaaga tgattctcag aaaacaaaaa gctagagatt cccaattgcg gaactctagc
     2101 tttttaattt tgcctctttc tcttattata tttcagcagg ttgttggcca tgagtatgaa
     2161 tcccatgtca attctcactt gacgcttacc tctcagatga catctcttat aacccaaaca
     2221 aacctttatc tgcccaaaga cagatttcat atcaatctta cgtttagcga aaatttgtct
     2281 acccttggaa gataaaagtg cctgatattc tttagttttt aaacactggt aacgttcatt
     2341 catatacagt cccttttgag gggctgattc aggttcataa tcgcagtcaa cattgatttc
     2401 aaggctgttt gctttctatc tccccttttt catgatatat tttcgtacag gtgcttcaat
     2461 agtttctata gtaaaatgaa ccaaaaatag tacacgttgc ggtataatgt tcttgtggca
     2521 tattcattag attttcgtaa aaagttctct cctatttgtg agcgaacagg tatagtcaat
     2581 tgattgtcag tttgaagacc aagagtcgta aggcaaagga tatggcagaa tccatccaag
     2641 ggtggctggc acaatttcta gtgaacctct ttaaatctat tacgtttgac tgtggaaaag
     2701 agttttcaaa atggaaagat atttccaatc atcacgatag tgagagcttt tttgcgaatc
     2761 taggatgtcc aagatagcgg tgtctcaacg aacattcaaa tagactttta aggtgtcatg
     2821 acttgccaaa gcagacagat ttcaacgaag tcagtcaaga attttaggct gggcaagaac
     2881 tcaatttcag gagaaaatga aataaatatt cccacaataa aacgcatagt atcaaagttt
     2941 ttccaacacc tgatattatg cattttttaa ttttaaagac tttttcccag cctcaagaaa
     3001 tcacatccca cctaaatcct ttggatacca aatacctcat caggttattt tctgagttgg
     3061 taagactgtc tagcttaatt tgacaaatgg agggcgttaa atccagtttt tacaggaagt
     3121 ctatttaaat attttcacac tgaccttaca aaatatgaaa taaattatat aaagtcattc
     3181 ccatgacaaa gaataacatc cctttaaaac agtatcttac ccaattttca ggtaagactt
     3241 ttgatacaac cgtccccaat acccctccgc atatagaagc aagaaaaata actggaacag
     3301 gtgctaaatg atactgattt aaacctgtaa ccacaacaat tgtagccaaa tgagacagct
     3361 gggaaaagaa aataattgca agagaataca tcgttgcttc ttttatcgaa atagaaaaaa
     3421 acaccatcaa taaagataca ttgagcggac ctccaccaat ccctaaaaaa ctagaaatca
     3481 agcccaatag aagaccacaa atcatataga atcctatctt atcaaattga taagtttttt
     3541 taaaatctgt taaaacaaat gatacaagta gcatcacaaa tagtaaaatc atttgaacaa
     3601 tggatactag ccgtactgat aattgagata acaaaacttg aaaaataaga tgacctagaa
     3661 atcctcctaa aacagaagaa aaggataccg taaaaatcaa tctccatttt atctctttac
     3721 tttgtgaaaa tcttttggtt gttgaaataa tcgccattat aaaaacagag aaactagaat
     3781 aaaaagcaat atcactgacc gaatggtatc caaaactatc cataagtggc ttaattataa
     3841 ttccaccacc catgcctgat atagaaccca atccactagc tattataatg attagaaaat
     3901 aaataatctc aaccactttt taagtccatc ctattctttc aaacatctaa aacctagatg
     3961 gctactagtg gaagtagaaa tacatccatt tctagcagct actcggtatc gattacaata
     4021 agaacaatga cataaaaagg aacctcctct gatagcatac tcctcatctt gtatcccata
     4081 ttttggcaac tcatagtcat cttcattaaa actagctaat aaggtatatc tcggattgcg
     4141 acaccattcc caaacattcc caatcatttg atatagacca aaatcattag gtaaaaattc
     4201 atagacagga gccgttccta taaatccatc taagccagta ttttcataag gaaatcttcc
     4261 ttgccaagta ttggcatgat aatatccacc ttctaaaaga gtatctcccc aaggatattc
     4321 agattgtctt cctcctctag cagcgtattc ccactgtgct tcagtcggta aagacattcc
     4381 gctccaatta cagaaagcca atgcatcttc taaagcaaca tgaactactg gatggtcttc
     4441 taaacccact agattactgt tctcaccgta gggatgtttc cagcaagcgt tcggaacttg
     4501 taaccaccac ggagccccgg caggatgagg atatccctcc ctttctgcct ccggaacgaa
     4561 caatataaaa acaaaagacc actcttgtcg ctcagctagt gttttatagc ctgtttcttt
     4621 cacaaattga gcaaattctt gattcgtaac tggagtatcc gcaatggaaa aatcctttac
     4681 actaacagcg acttgaggat cttcaaaatt atctaaaaag ccctcttcag aatttgttcc
     4741 catcataaat gtcccaccct tgattttttt cattttcatg attgactatc ccccgttttc
     4801 gttagagttg gcgttatttt agaaagttca acaggaacta actcttcatc cacaacaaaa
     4861 ccttcttctc tatatctaag aaagtttact aattttttct tcatctgacg aacaataggt
     4921 tggtattttt cacttggata caaatcattc atttcatgcg gatctttttt catatcaaat
     4981 aactgataat gatttaaaac aggaaaccag ataaatttcc actggtctgt caaaatatat
     5041 tgactagaat cttttcctaa agcatgttca ccatgaaatt ctgttcgcca cccctcgtac
     5101 tgtccaaata ataaattctt aacactccgt ccatctagtt catctgtagt agttcctcct
     5161 gccaaatcca cgagtgaagg aaaaatatct tgaattttca ctaattgctt gattgttccc
     5221 ctattaccag ctatcaatcc agccggatcg taaataaacg aaggaatgtg gatacttccc
     5281 tgataaggat atccctttct aaacagataa tgttcaccaa gctgatctcc atgatccgaa
     5341 acaaaccaga taatcgtatc tttatcatga cgaaattctt ttagggcagt caaaaagcga
     5401 ctaatttgat ggtctatatg agtaatcaat ccaaaataag cagcaaccat tcttctttga
     5461 tcatcttcct ttaatttccc tcttaaagca taaatagacg gaatttgctt ttctaatact
     5521 tcccaattcc cgatatgcaa atcgagaaat tgaggcaagc gttccatata catatcaaaa
     5581 tagtattttg ggggatttaa tggagcatgt ggtttttcaa aagacatttt gagaaaaaaa
     5641 ggaaccgttg gatctcttct ttgtaaaaat gaaatggact cactcacaac ccagtttgtt
     5701 ggatgtaact tttcatcttt atcccaaggt ctagcttccc aagaattaca atccattccg
     5761 tcatcaatca aatcaacatc ataaccaact ttacccttta aaaaggctag atagtcacta
     5821 gcataatcaa attgacttcc ataagcctta tcatactttc tatcaacatg tagataccca
     5881 tcgtgaagta agacatgatc aaacccaagt ctctgtctgg aaggaaaaac atgcatctta
     5941 ccaatacact ctgtttgata acccatatct ttaaagactt ttggaagagt attagtaaaa
     6001 ttccaaggca cctcatcttg ataaccaaca cgtccacttt tatcttggtc taagccagtc
     6061 aataaagctg ccctagcagg aacgcaagaa ggaactggag aataggcatt ttcaaaatta
     6121 taacctacac tagccatcat atctaatgtt ggagtgctaa ctagcttatc cttactattc
     6181 aaggataagg cgtctgctct catttgatct acaacaatca aaataatatt tggctgtttt
     6241 gtctgaacca taaaatctcc tttctaatat ggcaaaagag gcacaagaag atatctacct
     6301 ttactgcacc cctttctata tcaatctctc tatataaaac aataacattc ttgttatgtt
     6361 ttatataaaa atgaactaaa atatgactaa atcgattagg aaattcaaat cattttctag
     6421 tactgtttta gaagttacag tgtactattc caacttcaat aaactataaa cctttgtcta
     6481 ttaacaattt tagtggagat aagaaatcct acacctaact catcttacac gtaatctatt
     6541 tctattttat cacaaaaaac gcaagtaaga ccattaactc aattcagttt tatctgccat
     6601 tttcacaaat gggaaataag tcaagacact aataatcaaa caaacaactg ataagatgat
     6661 ggcatgccaa tcaaatgctg tagagaagaa accatataaa attggaggca ttacccaagt
     6721 aacattttgt gtaacaggtg aaacaagacc ccagcttgtt gcccagtaag ctaccgttgc
     6781 catgaaaacc gggctaagta caaatggtat aaatagcaaa ggattcaaga caactggtaa
     6841 accataattg accggctcac caatattaaa cagaactggt gctagaccaa gtttagcaac
     6901 ttttcgataa tgactgtttc ttgaaaaaat taaaatagca agtactaatc ctaatcctcc
     6961 aaaccagaca aacgccccaa aagacccact tgtccatata taaggaatcg gttcaccttt
     7021 ttggaaagca tccagattcg ctaacatagc aactccaaat agcccttcca tgatgggagc
     7081 caatacattt cctccatgga gaccaaaaaa ccagaataac ttattcaaaa agatcatcag
     7141 aataactgca aagaaacttt gagacaaacc tagtaatggc gtttgtaaca ccttgtaaac
     7201 ccaatcaatc aataagtcat tgctaagtaa atggaaaaca taagtcaaga tggctactat
     7261 atacatcgcc ataaatcctg gaatgataga agtgaacggc ttagcaatcg cagggggaac
     7321 tgaatctggt aacttgatta cccagttctt tttcattact ttacagaaaa taatagaggc
     7381 taaaaatcca atcatcatgg ctgtaaagta tcctctggca ttaatatggt ttcctggaat
     7441 cacattccca atagttacca tcagattttt accatcaaat gctagattat caattccttg
     7501 taagatttga tttaatttca catctcctac atttgccaaa gggaaactct ttgtaactgt
     7561 acttccaatc gaaatgacaa acgaagcaag tgataccaaa ccagcagaaa ctgtatcaac
     7621 cttgtaaatc ttagcgatat tcactcccaa gcaatagatg aacaataagg aaacaattgg
     7681 tatacttccc ttgaatacca aattattgat gtcaacaagc cactgaaagg ttttcgtaat
     7741 actttctagg tgaaattgtt gtggtaaatc cactagaaaa gcatttaata acaaagcaat
     7801 ggaacctgtc ataataacag gcatagtccc cacaaatgaa tcacgtaaag caactaaaaa
     7861 cttctgattc ccaactttca ttgcaattgg agaaagtttt tcttgcattt tatccaacat
     7921 tgtattcata gcaaattcct aatttttttc aaatcgttcg tatacttcta ccacttcttt
     7981 cgccaagtca acaaaggtca gactcgtcat taaatgatct tgcgcgtgta ccatcaagag
     8041 gctaattgaa gtttcttccc cctgtgcctc tcttgtcaac atttctgttt gagccttatg
     8101 cgcctgtaat aaggcagcat tcgcatcagc taatcttcga ttggctttag agaaatcccc
     8161 tttttttgct gcctgaatag cctccatagc attacttttg gcttccccac catacataat
     8221 tagccccatt gcagattcta aattactttc attcatagca ttctcctcaa tcttagtcta
     8281 ataaactttc agctaaatct aagactttag aaccattcat cattccataa tctgtcatcg
     8341 gaataaccgc cacaggaatc tgtctatctt ttagtttttc ttgaaagtcc cctagtaaat
     8401 agcgaacttg agggcctaaa agcaacacat ttacttcctt tgttgctaca atttcttctg
     8461 cttcaggagc tggaactgca aaaatttctg catccaaccc cttatcttct gctgcctttt
     8521 gcatctttgt cactagcaga cttgtactca tacctgctgc acatgctaac ataattgtta
     8581 ctttagccat agcttactcc tttatcacct atttactgac ctagccaagc gagatactgt
     8641 atgtctcact cctctaattg ccttgctcaa tgcataaata ttatcgactg ttgccagtcc
     8701 cccatatcct gcgtcaccta tatgttgaat atcaactcca caaattttat ttctaagtgc
     8761 aatttccttg atagtatctg tatcagatgt ttcttggcta gtaccaatag cactcagtac
     8821 tagcccaccc ttactatgaa cgagatcaac gacttcacgc aactcttggt catgaaaggc
     8881 tggaacggtt ccaactgctg gaacaagtat cacatccgca cctgcttcca acaattgctc
     8941 tgctacagaa agctctgcca caggctcatt cactcctgcc ccgtgcatct tacctgcaat
     9001 aatcaaacca gaaaagtttt ccttagcagt ttgaacagcc ttaatgattt ctcgattgct
     9061 aactcctgtt ccaggatttc cagtcaaaca gacaaagtca aaacctaact cctctattcg
     9121 attcaatgtt tcaacactag caacacgacc tgcaacaatt tcctgtgttt cctctagcat
     9181 ctttgcagat gggtcaatcg gttccaaatt tacaccaatt ggacaagcaa caagctggtg
     9241 taagcgatga ataacatcat cacctgaact atccaaagca taaattttag gctcaaaaac
     9301 atcaacacaa ttcaataaaa tcatatcagc tccaaaagct ctagcaattt cagaattagt
     9361 tatatcccca acaaatgtct cacgagtaac tacattttca gatagaacaa ctctaccttc
     9421 acttgctaaa atactttgtt ttaattcttc agcattcatc tgtaatatct cagatggatt
     9481 tgcactaatt aaacgtttca ttctcaatcc tccaataact taaaccgttt ttcaatagct
     9541 attacaaatg gtaaataaag taagacatcc aaaacaatca atacaatttg taataatgaa
     9601 ccagaaatac ttcctgtagc aagtaaacca cttataacag gtggcatagt ccaacctgga
     9661 tctgtataag ttagcggaac caaacctgat gccattgctc cccacgctac tagtaagtta
     9721 aacattggag ctaagataaa tggaactagt aacaaaatgt tcaaaacaat aggaacgcca
     9781 aacattgctg gttcatttat attaaataat ccaggtataa cagttatagg tgccaaagtt
     9841 tttagttgtt tactagcttt cttcttatgt gctaaatatc ctaacgcaag aactaaacct
     9901 atggttgcac caccgccacc tatgaataca aaattatcca taaatacgct agtaaaaata
     9961 tttggaagag tttctccagt ttgataagct actttatttg catctagatt agctaaccat
    10021 aacggtttca taactgcatt aacaacattt cctccgtgta atcctacaaa ccaaaaacta
    10081 ctattaagga aaattaagat aagaagacca ataacattat ttcctaaaag tccaagtgga
    10141 cctcccaaag ctacttgagc aatttcatga agattaggta aacttaattt atctaaagtt
    10201 gcataaacaa tcaaccaacc aacaatagtc actgcaccag gaataatggc actaaagctt
    10261 ttagatactg ctggtggaac accatccggc atttttatct gtatattatg atttataaac
    10321 cattggtaga tatagccatt aatcaatcct aaaatcatag caacaaataa accttttgat
    10381 gccatgaaag cggtaggcat tcctgctcca gcctctcctc taatgaaagg cgtaactgta
    10441 ataaaagatg ataatgacac aataccagca ggaataccat caactttgta ttgtctagcc
    10501 ataaagtaag cgataccaaa actagctaca agaccaagta atccaaaact actatcaaca
    10561 cccttccata aatagtcagc aacaccaata tcacccagat atttttccca tcctggaata
    10621 ggaaaacttg caataaccat tagtaatgaa cctatcaaca ataaaggtat agttagagta
    10681 ataccgtctc gaatagaaac caacgcttta ttattaccca atctagccgc tactggaagt
    10741 aacttttctt gtaataattt ttccataacc ttttcctctt aaatatattt tattatcaaa
    10801 agttatcaat tatctttatt ttttgacgtc aagttgttat
//
LOCUS       DAVLVF010000061        10672 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVLVF010000061 DAVLVF010000000
VERSION     DAVLVF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10672
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.061"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            210..1997
                     /locus_tag="VYN66_001492"
     CDS             210..1997
                     /locus_tag="VYN66_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV1495497.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRIPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLNNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            1994..2470
                     /locus_tag="VYN66_001493"
     CDS             1994..2470
                     /locus_tag="VYN66_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1495498.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2498..3403
                     /locus_tag="VYN66_001494"
     CDS             2498..3403
                     /locus_tag="VYN66_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1495499.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3390..4205
                     /locus_tag="VYN66_001495"
     CDS             3390..4205
                     /locus_tag="VYN66_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1495500.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALTLSALGHFALGM"
     gene            4212..4616
                     /locus_tag="VYN66_001496"
     CDS             4212..4616
                     /locus_tag="VYN66_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1495501.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIGGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4914..6080
                     /locus_tag="VYN66_001497"
     CDS             4914..6080
                     /locus_tag="VYN66_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV1495502.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKKLVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALDCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6196..7233
                     /locus_tag="VYN66_001498"
     CDS             6196..7233
                     /locus_tag="VYN66_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV1495503.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGALLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7440..8294
                     /locus_tag="VYN66_001499"
     CDS             7440..8294
                     /locus_tag="VYN66_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1495504.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            8444..8701
                     /locus_tag="VYN66_001500"
     CDS             8444..8701
                     /locus_tag="VYN66_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV1495505.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADLVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(8743..9060)
                     /locus_tag="VYN66_001501"
     CDS             complement(8743..9060)
                     /locus_tag="VYN66_001501"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495506.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDRTDEVSNHIYGKVKLTWFEEI
                     FEEYKSPHNKTHTIKVFMYCPPKVRQKKSNFWGQYISIPDTMRFFNFKDFLQLLLTHI
                     KLF"
     gene            complement(9132..9410)
                     /locus_tag="VYN66_001502"
     CDS             complement(9132..9410)
                     /locus_tag="VYN66_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1495507.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNA"
     gene            9632..10396
                     /locus_tag="VYN66_001503"
     CDS             9632..10396
                     /locus_tag="VYN66_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV1495508.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
BASE COUNT         2977 a         2014 c         2452 g         3229 t
ORIGIN      
        1 tctatacaaa attattttag gtcctatatt ccgaatttaa agagcgtttg aaatgggtct
       61 aaaaatgttt gaaactaatt gaatgtgtgt ataatagtaa aaatgcagta aatatcccga
      121 caatttctat tgacaattca aacagattgg tttataatta atataacaac aaatgaaagc
      181 gcaaactttc gcggtcggaa ggtagtttta tgacacgatt tgagatacga gatgatttct
      241 atctcgatgg aaaatcattt aagattttat ctggtgccat tcattatttt aggattcctc
      301 cagaggattg gtatcattcg ctctataact tgaaggctct tggttttaat acggtagaga
      361 cttatgttgc ttggaattta cacgagcctc gtgaaggtga gtttcatttt gaaggtgatc
      421 tggatttaga gaaatttctc caaatagcgc aggatttggg tctctacgca attgtgcgtc
      481 cgtctccatt tatctgtgcg gaatgggaat tcggtggctt accagcttgg ctcttgacca
      541 agaacatgcg aattcgctca tccgacccag catatatcga ggcagttggt cgctactatg
      601 atcagttatt gccaagactg gtgcctcgtt tgttgaacaa tggtggcaat attctcatga
      661 tgcaggttga aaatgagtat ggttcttacg gagaagataa ggcttacctg agagcgattc
      721 gacagctaat ggaagagtgt ggcgtaacct gtcccctctt tacatcagat ggtccatggc
      781 gagctactct gaaagctgga accttaattg aagaggacct ctttgtaaca ggaaactttg
      841 gttctaaggc accttacaac ttttcgcaga tgcaggaatt ctttgatgaa catggtaaga
      901 aatggccact catgtgtatg gagttctggg atggttggtt caatcgctgg aaagaaccga
      961 ttatcacacg ggatcctaag gaattggcag atgcagttcg agaggttttg gaacaaggct
     1021 ctatcaatct ttacatgttc cacggtggta caaactttgg tttcatgaat ggttgctcag
     1081 ctcgaggaac tttggacctg ccacaagtta catcttatga ttacgatgcc cttctggatg
     1141 aagaaggaaa tccaactgct aaatatcttg cagtcaagaa gatgatggca acacattttt
     1201 cagagtatcc gcagttggaa ccactctaca aagagagtat ggagttggat gctattccac
     1261 tagttgaaaa agtttctttg tttgaaacct tagatagctt gtcaagtcct gtagaaagtc
     1321 tctatcctca aaagatggag gagctgggac aaagttatgg ctacctactt tatcgaacag
     1381 aaacaaactg ggatgcagaa gaagaaagac ttcgtatcat tgatggtcga gatagggccc
     1441 agctgtatgt cgatggtcag tgggttaaaa ctcaatatca gacagagatt ggggaagata
     1501 ttttttatca aggtaaaaag aaagggctat ctaggttaga tatcttgata gaaaatatgg
     1561 ggcgtgtcaa ctatgggcat aagttcttag cggatacgca acgtaaggga attcggacag
     1621 gggtctgtaa ggatctgcat ttcttactaa actggaaaca ctatccactc ccactagaca
     1681 atcctgagaa aattgatttt tcaaaaggat ggactcaagg acaaccagcc ttttacgctt
     1741 atgactttac agtcgaagag ccaaaagata cttacctaga cttgtctgag tttggtaagg
     1801 gagttgcctt tgtcaatggg cagaatctag gacgtttttg gaacgttggc ccaactctct
     1861 cactttatat ccctcatagc tatctcaagg aaggtgccaa ccgtatcatt atctttgaaa
     1921 cagaaggtca atataaagaa gagattcatt taactcgtaa acctacacta aaacatataa
     1981 agggggaaaa cttatgacaa ttgtaggatg ccgtattgat ggacgtttga tccacggaca
     2041 agtagccaat ctttgggctg gaaaactaaa tgtttcacgc attatggttg tagacgacga
     2101 agttgtcaac aacgatattg aaaagagtgg tttgaaactt gcgacaccac caggtgtgaa
     2161 attgagtatt ttgccagttg agaaagctgc agccaatatt cttgctggta aatacgatag
     2221 ccaacgtctc tttatcgtgg ctcgtaaacc agaccgcttc cttggtttgg tagaagcagg
     2281 tgtaccactt gaaaccctta atgttgggaa tatgtctcaa acaccagaaa ctcgttctat
     2341 tacacgttct atcaacgtag tagacaagga tgtggaagac ttccacaaac tggcagaaaa
     2401 aggtgttaaa cttactgctc agatggttcc aaatgatcca atttcagact ttttgagctt
     2461 attaaaatag gaaaaaaatt tttaggaggt cattgttatg atacaatggt ggcaaatttt
     2521 acttctcact ttgtactcag cttatcaaat ctgtgatgag ttgacgatcg tttcatctgc
     2581 aggttcccct gtatttgctg gtttcattac tggtttaatc atgggagatg tgactactgg
     2641 tttacttatc ggtggtaact tgcaactgtt cgttcttggg gttggtacct tcggtggtgc
     2701 ttctcgtatc gacgcaactt ctggtgcggt tcttgcgaca gccttctctg tttcacaagg
     2761 aattgatgca ccgcttgcca ttactacaat cgctgtacca gtagcagctc tcttgactta
     2821 cttcgacgtt cttggtcgta tgactactac cttcttcgct caccgtgtgg atgctgcaat
     2881 cgaacgcttt gactataaag gtattgaacg caactacttg cttggtgcga ttccgtgggc
     2941 tctatctcgt gcccttccag tcttctttgc ccttgctttt ggtggtgcct ttgtacaatc
     3001 agtagtagac ttcgttgaag cctacaaatg ggttgcagat ggcttgacac ttgcaggacg
     3061 tatgcttcca ggtcttggat ttgcaatctt gcttcgttac cttccagtta aacgtaacct
     3121 tcactacctt gctatgggat ttggtttgac agctatgttg actgttcttt actcatatgt
     3181 aacaggtctt ggtggcgctg ttgctggtat cgtaggtact cttcctgctg aagttgctga
     3241 aaaaattggt ttcgtgaaca acttcaaagg tttgtctatg attggtattt ctatcgtagg
     3301 tattttcctt gcagtgcttc acttcaaaaa tagccaaaaa gtagctgtag cagcaccttc
     3361 tacaccatca gaaagtgggg aaatcgaaga tgacgaattc taattacaaa cttacaaaag
     3421 aagattttaa tcaaatcaac aaacgtagct tgtttacttt ccaattaggt tggaactacg
     3481 aacgtatgca agcttctggt tacctttaca tgatcttgcc tcagttgcgt aaaatgtatg
     3541 gtgatggaac tcctgaattg aaagaaatga tgaaagttca tactcaattc ttcaatactt
     3601 caccattctt ccataccatt atcgctggtt ttgaccttgc catggaagaa aaagatggtg
     3661 taggttcaaa agacgctgtt aacggtatca agacaggttt gatgggacca ttcgctcctc
     3721 ttggggatac aatctttggt tcacttgtac ctgctatcat ggggtcagtc gcagcaacta
     3781 tggctatcgc tggccaacct tgggggatct tcctttggat tgcagttgca gtagcgtatg
     3841 acatcttccg ttggaaacag ttggaatttg cttacaaaga aggggttaac cttatcaaca
     3901 acatgcaaag taccttgaca gctttgattg acgctgcatc tgtacttggt gtcttcatga
     3961 tgggtgctct tgtagcaaca gtgattaact ttgaaatttc ttacaagttg ccaatcggtg
     4021 aaaagatgat tgatttccaa gacatcttga accaaatctt cccacgtttg cttccagcaa
     4081 tctttactgc ctttatcttc tggttgcttg gtaagaaagg tatgaactct actaaagcta
     4141 tcggtattat tatcgtactt gctttgactc tttctgccct tggtcacttt gcacttggaa
     4201 tgtaattcct tatgactaaa tcattaattt tggtgagcca tggtcgcttc tgtgaggagc
     4261 ttagaggtag cacagaaatg attatgggcc cacaagacaa catttacaca gtagctcttc
     4321 ttccagaaga tggcccagaa gaatttactg ctaaatttga agctgttatt ggaggattgg
     4381 atgatttcct agtctttgcg gatcttctcg gtgggacacc ttgtaatgtg gtgagtcgct
     4441 tgatcatgga aggtcgtgat attgaccttt acgcagggat gaatcttcca atggtgattg
     4501 aatttatcaa tgcgagcctt acaggcgcag atgcggacta caagagccgt gctgcagaaa
     4561 gcattgtgaa agttaatgac ctgttagcgg gcttcgatga tgacgaagat gaataatact
     4621 cttcgaaaat ctcttcaaac tacgtcaacg tcgccttgcc gtaggtatat gttactgact
     4681 tcgtcagtct tatccggcaa cctcaaaacg gtgttttgag ctgacttcgt cagttctatc
     4741 tgcaacctca aagtagtgct ttgagcagcc tgcggctagt ttcctacaga ttttagttgg
     4801 aactcgattc aattcatgtg acaacgtgaa aatcgttaga gcattttata tagaatatac
     4861 atgggaatgg agcttactcc cattcccata tttaatagaa aaagaggaac tcaatgctac
     4921 attatacaaa agaagacttg ctcgaattgg gtgcagaaat cactacgcgt gaaatctacc
     4981 aacaacctga tgtatggaga gaagcttttg aattttatca agcaaaacgt gaagaaattg
     5041 cagccttcct acaagaaatc gctgataaac atgactatat taaggttatc ttgacaggtg
     5101 ctgggacttc tgcttatgtg ggagacacct tgctacctta ctttaaggaa gtctatgacg
     5161 aacgcaaatg gaatttcaat gctattgcga caacagatat cgttgccaat ccagcaacct
     5221 atttgaaaaa agatgtggca actgtccttg tgtcttttgc tcgtagtggg aactcgcctg
     5281 aaagtgtggc gactgttgat ttggccaaat ccttggtgga tgagctttat caagtgacga
     5341 ttacttgtgc agcagatggt aaattggctc ttcaagctca cggtgatgac cgtaatctct
     5401 tgctcttgca accagctgtc tctaatgatg ctggatttgc catgacttct agctttacgt
     5461 ctatgatgtt gacagctctc ttggtctttg atcctacaga atttgctgtt aagtctgaac
     5521 gttttgaagt tgtatctagt cttgcccgta aagttttaga caaggcagaa gatgtcaaaa
     5581 agctcgttga tttagacttt aaccgtgtca tctatctagg cgctggtcct ttctttggac
     5641 ttgctcatga agctcagctc aagattttgg aattaactgc tggtcaagtt gcgaccatgt
     5701 atgaaagccc agttggcttc cgtcacggtc caaaatctct tatcaacgac aatacagttg
     5761 ttttggtctt tggtacaacg acagactaca ctcgtaagta cgacttggac ttggttcgtg
     5821 aagttgctgg tgaccagatt gctcgtcgtg ttgtgctttt gagtgatcaa gcttttggtc
     5881 ttgaaaatgt caaagaagtg gcccttgatt gtggcggtgt cttgaatgat atttaccgtg
     5941 tcttccctta catcgtttat gcccaactct ttgccctatt gacttcactc aaggtagaaa
     6001 ataaaccaga tacaccgtct cctacaggta cagtaaaccg tgtagtacaa ggtgtcataa
     6061 ttcacgaata tcaaaagtaa gacagtgttt atgaattctt gacaagagga tttgtaaatt
     6121 atcagataaa ccatagattg tcagtacgct ttctatggtt tgtttgcttg agagaaatag
     6181 taaaaggaga acagaatgaa agcatacaca gagcgtgtat ttggaaatgt tgagggcgag
     6241 gatgtcttgg cctatcgatt tgagacagac ggtggctacc aacttgaggt tatgacttat
     6301 ggcgcgacca tcttgcgcta tgtcgcacct gacaaggctg gaaattttgc caatgttatc
     6361 ttgggatttg atgactttga tagttatgta ggcaatagtc ccaagcatgg agcaagtgta
     6421 ggtcctgtag cgggtcgtat tgcaggtgcg acctttgagc tcaatggtaa gacctatgac
     6481 cttgaggtta ataatgctag caactgtaat cacagtggtt caactggttg ggattccagc
     6541 ttgtttgaag ttgaagaagt aagcgatcat ggcttgactc tctacacaga gcgtacagat
     6601 gggacaggag ggttccctgg aaatctcaag atttggatca gttatcactt ggaagaaact
     6661 ggtgcctatg aaatcagcta caaggtaacg accgatcagg atacgctggt caatccaacc
     6721 aaccacagct atttcaactt gtctggtgat ttcacgcaga cgattgaccg tcatgtcttc
     6781 caactaaaca cagagggcat ttactcaatc gctcctgacg gtgttcctgc caaaactcca
     6841 gaagccaacc gtgatgtggt caaacacatc tacaatggtg ccttgttgaa ggatatcttt
     6901 gcagaagaag atgagcaaat ccagctggca tcaggtttgg atcatccatt tgcccttcct
     6961 gcaggccatg acaatgctgg attcctttat gaccaaaatt caggtcgctt cctgcttttc
     7021 aagacagaag ctccttgctt tgtggtctac acagcaaact ttgtggatga aagtgtcatc
     7081 ataggaggtc agccaatgct acagcacaat gggattgctc ttgaagcgca agctttacca
     7141 gatgccattc acagtgacct taaaggccaa gtcattctta aagctggtca aaccttcacc
     7201 agtaagacac gttatgaact tgttgtgaaa taaaaaagct ggtcaaaaag tctttaaaat
     7261 taaaataacg catattgtca ggtgatgaaa agtttgataa tatgcgtttt attatgaaat
     7321 gatttacttt atattatcct gaaatggtgt tatttttgag ttatctggct tgactttctt
     7381 gatggaaatt atataatagt tgcaacaata ataattacaa cgaaaaaaga ggaccattta
     7441 tgacctatga atacaagagt cacatttatt tggcagagac agttttaaat gtaaaggatt
     7501 tggccagtca aacagccttt tatcagcaag ttatcggttt agaaatctta tctcaaactg
     7561 agacagagtc cattctaggc cttggtggaa aagtcttggt acagctgatt caagcacaag
     7621 agagcggaga agtgagggaa cattatggtc tttaccattt ggccattctc ttgcccacac
     7681 gcaaggcttt ggctgatgtc ttgaaacacc tgacagattt acagattcct cttgtcggcg
     7741 gtgcagacca tggttacagt gaagctattt acttggaaga tttggaggga aatggcattg
     7801 aactctatcg agataagcca gtttccacat gggatattcg agaagatgga cgcattatcg
     7861 gagtgacgga ggctcttgcg gcgcaggaca tttatgagtt gggggaaaga gtagagccct
     7921 ttatcctggc agaaggtacg agaatggggc atattcatct ttcagtcaag gatagtcgaa
     7981 agtccagcca gttttatcaa aaggtgttag ggctagagga taaattcagt gtgcctagcg
     8041 ctagttggat tgcggctgga gattaccatc atcatttagc agttaacgaa tggggaggaa
     8101 aaggtctggc ttcgcgtaag caaggcttac caggtttagc ctactatgtc atcgaagtcg
     8161 cacataaaaa agaactgtta acgattgccc aacgagcaca agaagttgac gcaccaatca
     8221 aatggatgac atcgagccaa ttggaaatca cagactcaga tggcatcgtg acccgtattc
     8281 gtttagcaag gtaaaattag gaatttttaa taagttataa gtattgctag tttgcttttt
     8341 gaacggtctt tggtaacaga actgcgtgag ctggatgggt gagaattttg acaaagaagc
     8401 tgattagaga tataatgaag aaaagtcgag caaggaaaaa gaaatgacaa attcaaagta
     8461 tattacttgt ttgaaacgtt cagagggcca gttgtgtggg attcaaaaga tgattgaagg
     8521 agatcgtgac tgtgctgatc ttgtgacgca actgacagca gtgagatcta gtgtagagcg
     8581 cgtgattgag atgataatta ccgaaaatct tactgaatgt atcaatcagc cactagatga
     8641 ttctgaagct caaaaggaac gcttagaaaa ggctatccga tacttgatta aacggaaata
     8701 aatgaatgtt ttgagacgat atgtgcaaag gtctattaac tattagaata gtttaatgtg
     8761 agtcaaaaga agttgtaaaa aatctttaaa attaaaaaaa cgcatagtat caggtattga
     8821 aatgtactgc ccccaaaagt tagatttttt ctgtctaact tttggggggc agtacataaa
     8881 aaccttgata gtatgcgttt tattgtgggg agatttatac tcttcgaaaa tctcttcaaa
     8941 ccacgtcagc ttcaccttgc cgtagatatg gttactgact tcgtcagttc tatccacaac
     9001 cttaaaacag tgttttgagc agcctgcggc taacttccta gtttgcactt taattttcat
     9061 tgagtattac ttcatcttct catgaaattg agtttttgcc cagctcctct tgattattct
     9121 acttgaccgg gctaagcatt cataccacct tctacgttgg taacggtgag gccttgggag
     9181 ctgagaaatt ggcaggtaga tgcagagcgg actcctcctt gacaaataac atagtattca
     9241 tggtcaggtt tgagttcttt gtagccttgc tctaaggtac ttaatggaag atttttggca
     9301 cctggtgcat gtcctgcttg gaattcatgt gcttcacgga catcgataag ttctagattt
     9361 tcattttgat attttccata aaagtcagcc atgctgatac tagtttccat attctactcc
     9421 ttctgggtta gccattttgt atagtgaata agcgccgtca aggttttgga cggtaaatcc
     9481 tgcttgcttg aggataggct ctgcgatata gctgcgcaaa ctactgtggc aactgacgat
     9541 gtaggcttgt ttttctcctc agttcatcag cttcctcctt gacactcggt cagattttga
     9601 tacaatagta caaaattaga ggaggtgggc tatgattcag aaacatgcga ttcccatttt
     9661 agagtttgat gataatcctc aggcggttat catgcccaat cacgaggggt tggatttgca
     9721 gttgccaaag aagtgtgtct atgctttttt aggtgaggag attgaccgct atgcgaggga
     9781 agtaggggcg aactgtgttg gcgaattcgt ttctgctacc aagacctatc cagtctatgt
     9841 catcaactac aagggtgagg aggtctgtct ggctcaggct cctgttggct ccgctccagc
     9901 agcccagttt atggattggt tgattggcta tggtgtggag cagattatct ctactgggac
     9961 ctgtggtgtc ctagctgata tagaggaaaa tgcctttcta gtccctgttc gcgctttgcg
    10021 agatgaggga gccagttacc actatgtggc accttgtcgt tatatggaaa tgcagccaga
    10081 ggctattgct gctattgagg aagttttgga agacagaggg attccttatg aagaagtcat
    10141 gacctggacg acagacggtt tttaccgaga aacggctgaa aaggtggctt atcgtaagga
    10201 agaaggctgt gctgttgtgg agatggagtg ttctgctctt gctgcagtag ctcaattgcg
    10261 tggggttctc tggggtgaat tgttgttcac agcagattct ctagcggact tggaccagta
    10321 cgacagtcgt gactggggct cggaagcttt taataaggcg ctagaactga gtttagcaag
    10381 tgttcaccac ctttagttgt actggcaaag gatttgtttt atcataaaat gtctagctca
    10441 tacttttcaa aaatatgttt aaacgaggtc accttcctct tgtcctaggc atgttgaggt
    10501 tgggaaaaat ctttaaaatc agaaaaacgt atcatatcag gtgatgaaaa ttttgatact
    10561 atgcgtttta tgttggtaag atttagagtg agatgaaatg atactcttcg aaaatctctt
    10621 caaaccaggt cagcttcacc ttgccgtata gatgttactg acttcgtcag tt
//
LOCUS       DAVLVF010000062        10636 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVLVF010000062 DAVLVF010000000
VERSION     DAVLVF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10636
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.062"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..226
                     /locus_tag="VYN66_001504"
     CDS             <1..226
                     /locus_tag="VYN66_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1495509.1"
                     /translation="LSEVFPEEHIYYAHAYSSWERGSNENYNRLIRRWLPKGTKKTTP
                     KEVAFIENWINNYPKKCLDYKSPSEFLLGG"
     gene            complement(255..1469)
                     /locus_tag="VYN66_001505"
     CDS             complement(255..1469)
                     /locus_tag="VYN66_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1495510.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1625..1919)
                     /locus_tag="VYN66_001506"
                     /pseudo
     CDS             complement(1625..1919)
                     /locus_tag="VYN66_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2081..2533
                     /locus_tag="VYN66_001507"
     CDS             2081..2533
                     /locus_tag="VYN66_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV1495511.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2568..3956)
                     /locus_tag="VYN66_001508"
     CDS             complement(2568..3956)
                     /locus_tag="VYN66_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1495512.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            4022..4984
                     /locus_tag="VYN66_001509"
     CDS             4022..4984
                     /locus_tag="VYN66_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV1495513.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(5190..6200)
                     /gene="ccpA"
                     /locus_tag="VYN66_001510"
     CDS             complement(5190..6200)
                     /gene="ccpA"
                     /locus_tag="VYN66_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV1495514.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLVKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(6624..7223)
                     /locus_tag="VYN66_001511"
     CDS             complement(6624..7223)
                     /locus_tag="VYN66_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1495515.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLAFQADVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(7224..8321)
                     /locus_tag="VYN66_001512"
     CDS             complement(7224..8321)
                     /locus_tag="VYN66_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1495516.1"
                     /translation="MLERLKSIDYMYWASLIFMIFPILPVVTGEIPSWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGLMLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     VKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKAYLKLERAEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVSPFSGEVSVISQKHPTEVQVRLPYKERK"
     gene            complement(8321..9058)
                     /locus_tag="VYN66_001513"
     CDS             complement(8321..9058)
                     /locus_tag="VYN66_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1495517.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAMFSFPAVLHTDKINNWQKTLRHTPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHFVRGVDMPLGSWIGAAFLLIVGSVAFVALGLTLTLLPSS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGGINL
                     SATVYILVFSAVLFGLTIYLQGHKENA"
     gene            complement(9060..9944)
                     /locus_tag="VYN66_001514"
     CDS             complement(9060..9944)
                     /locus_tag="VYN66_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495518.1"
                     /translation="MNMIKVESLNKNIKGKAILKGISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLSVTSSQLDYIRGYLPQENVIVQKLKVKELIAFFQRIY
                     PNSLSDQEIDQLLQFDQQQKEQFAEKLSGGQKRLFSFVLTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     GIEKHFILPLAYKEVIEQSNLVENWSQKQDALQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(9931..10125)
                     /locus_tag="VYN66_001515"
     CDS             complement(9931..10125)
                     /locus_tag="VYN66_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495519.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTGWFDQTLLRQFIRGALGFGFM
                     IFVVFLMRMEWLKGEYHEYD"
     gene            complement(10122..10253)
                     /locus_tag="VYN66_001516"
     CDS             complement(10122..10253)
                     /locus_tag="VYN66_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine aminotransferase"
                     /protein_id="HEV1495520.1"
                     /translation="MFSGLDESFYHFPWELFAGFGMMSWLVREGLKLVGDVKKEFEE"
BASE COUNT         3238 a         2434 c         1936 g         3028 t
ORIGIN      
        1 attgtctgag gtatttcctg aggaacatat ctactacgca catgcttact cttcatggga
       61 gagaggttca aatgaaaatt ataatcgatt aattcggaga tggttaccta aaggaaccaa
      121 gaaaacgact ccgaaagaag tagcttttat cgaaaattgg attaacaact accctaaaaa
      181 atgcttggac tacaagtcgc caagtgaatt tcttttgggt ggctaacttc aacttgaaat
      241 ttgggatgca agccctatct acgatactgt ttcaagaaac gagtcatctt ttcttggatt
      301 tgggctaact catcaacacg aggaaggtaa acgatacgga agtggtctgg ttcctgccag
      361 ttaaagcctc gaccatgaac caagagaacc ttttcctgct tcaagaaatc aaggacaaac
      421 tgctcatcat catcgatacg gtacatattg cggtcgattt ttgggaagat atagagcccc
      481 gccttgggtt taacggcaga caaacctgga atatcttgaa tggcattata gatgaaattt
      541 ctttgctcgt agattcgtcc accaggaaga agcaattcat cgactgattg gtgacccccc
      601 aaggaagttt gtacgacttg ttgggccaaa acgttagagc aaaggcgcat attggacagc
      661 atattgagcc cttcgatata gcccttaaca tgagtcttag ggccagacaa gaccatccaa
      721 cccacacgga aacctgctat gcggtgggat tttgacagac cattcatgct gacacagaag
      781 acatctggtg ccaagctcgc cacaggcgta tgcacatgtc cgtccattac catgcggtca
      841 tagatttcat ccgcaaagat aatcaaatcg ttttgacggg caatctcaat aatctccagc
      901 aagagttcct taggataaag ggctccagtt gggttatttg gattgataag gacaattgcc
      961 ttggtattgg aagtgatttt tgacttgata tcgtcaatat ctgggtacca ttccacagct
     1021 tcatcacaga tatagtgaac ggcatttcct ccagctaggc tgacagccgc cgtccataga
     1081 ggatagtctg gcattggcac taagacctca tccccattgt ccaaaagccc ctgcatggac
     1141 atgacaatca actcactgac accatttcca aggtaaatat catcaatatc tacgtttggg
     1201 aatttcttca gctggcaata ctgcatgatg gccttacggg ctgagaaaat ccctttggag
     1261 tcagaatagc cttcactatc acgcgcattc ataatcaagt catgaatgac ctcgtctggc
     1321 gctgtaaagc caaattctgc tggatttcct gtattcagac gtaaaatctt ttctccattc
     1381 gctcgcatcc gcatggcttc ttccaataca ggaccacgga tatcataagc aacatgctct
     1441 aacttactag acttgttata ttctttcatc ttgtttcctc aattcatcag gatagtaaca
     1501 ttataccact agaaagagaa tgcgacaagt ttactgaaat gtgttactag tttcgtatag
     1561 aagaaaaacc ttgctaaagg cgaggttaag aacgtagaga tagcaggtat tttgcaaaga
     1621 cacatcacat accaaaagat aaacctagaa aaaagcaagc ctagaaagga gtcataaaga
     1681 agattaaaca tgaggaaaag cccccatatc atcaagtaat cccaacgccc acttcgtttt
     1741 gcaactagaa ataagattag atggtagagt acatgaaaaa ctaaaaatcc aataaaactt
     1801 ggataaagaa gcggtaccag actctctaat tgacagatca tgatcacttc taccaagact
     1861 gaaactagga taattccata ataaaggaaa tttgattttt gaaaagagtt tttcatcatt
     1921 ctcccttcct tcacttcttt cctgctatcc tttcttttat tttatcatat atttatatag
     1981 agtttaagcc ctaatattct tttgaaaaat actttttttg ctttacttct atagtgaaaa
     2041 ggtttacaat tatagtaaga aaatttcagg aggtttcatt atggcacaac gttaccaaaa
     2101 tatcatggtc gcaatcgatg gttctaagga agcggacttg gcttttgtca agggagttca
     2161 ttctgctcta cgaaacgacg ctaaactcac catcgcacat gtcattgaca cacgcgctct
     2221 ccaaagcgta tccacctttg atgctgaagt ttacgaagaa ctccaagtcg acgctgaaag
     2281 tctgatgaaa gagtacgaaa aacgtgctaa agatgctggg gtagcagatg ttcatatcgt
     2341 cattgaaatg ggaaatccaa agaccctgct agcacgtact attccagatg ccgaggaagt
     2401 ggacctcatc ctcgttggcg caactggtct caacgccttt gaacgcctct tggtcggctc
     2461 ttcatctgaa tacatactcc gccatgctaa ggtcgatttg ctggttgtga gagaacaaga
     2521 aaaaacctta taatcacaaa gaaaaggagc ccctagctcc tttttgttta cgatttattt
     2581 ctctctttat ggcgttcgta agccttgagc tggcgctgca gttccttttt aatagcaggt
     2641 tctggagcat atttttcttc ccaattatct ggttttaaga ttttatgggt cactggatca
     2701 aaatgagcct tgccatctgg aaaaattttc cccatattgg cctgatggac aatatcaaaa
     2761 atacgttctg ggtccacccc catcaagaca aaactgccgt aggtgaagta aagcgtgtca
     2821 atcaaggcat ccacttgccc tatcaaatct tgctgagcag gtgtcttctt ggctacttta
     2881 cctgctgcct tatcaagggc ctgatgaagt tgcgatacag cttgaccaaa atcttcttca
     2941 gaaggactgg ctgatcgaac aaactccacc aattcttcta ttttaaaacc agccctatgg
     3001 gttgcaccct ctaaatccca agctcgaggt tcttcttggg ttcgttcatc catcatgtgg
     3061 tggaaggtct tgaccttatt gaaatgatag tcacggctga caaaaacttt ttctgaagac
     3121 aaaacaccaa aatgttccaa ggccttgtgg atcccatctt gttgattgct agtggtaatg
     3181 tgcttggcag cttccttgac actgctacta ccatttccca tagcgaccga cataccgacg
     3241 ccagccagca tttcaaggtc attatctgag tcaccaaaag ccatgacttg gttgaggtca
     3301 aagccatatt ctttcccaac tcggcgaatg ccttctaatt tagaatttcc ctgattgatg
     3361 acatccgatg caaaaggatt gctacgtgtc aatttcaagt cttcaaaatc agctgccgcc
     3421 ttctcagatt cttctggtgt catcagcatc aaaacttggt aaataggttg attcatcagg
     3481 tgaagcaggt cctcttcctt ttggggaaca accttgctga ccatgcgatt aaaagactga
     3541 ctcaccgtcc gagttaaaac agagggaacg aagcgactaa ttcgttggga aaaagaaccc
     3601 agaccaaagg acatgatttt agaacccaac atggcatcct tggtccctaa ggcaatctcc
     3661 gtgccctctt ttttagcata gctaattaga tggcgcaaat gtaacttgga aatagggctc
     3721 gtgaacaaga ctctgtcttt actaaagata tactggccat tataggttac cgcaaaatcc
     3781 agatccaaat cgtccatcaa ttccttaaca aaaaaaggtc ctcgccctgt cgctacgcca
     3841 actagtaccc cttgttcttt gacaatctta atcgcatcct tagtagattt caaaacactc
     3901 ttgcgattgt tgaccaaggt tccatcgata tcaaaaaaaa cagctttgac ttccatccta
     3961 tcccaatctc cccttttgtg atacaatgat tataccacat ttcagaaaga gtgagtaaat
     4021 catgcctaag aaaatccttg ttttacatac gggtggaact atttccatgc aggccgatgc
     4081 ttctggcgct gttgtgacga gttcagataa tcccatgaac catgtgtcca acccacttga
     4141 aggaatccaa gtccacgcct tggacttttt taaccttcca agtccccata tcaaacccaa
     4201 acatatgctg gtcctctacc agaaaattaa agaggaagca gataactacg atggagtggt
     4261 gatcacacac ggaaccgata ctttagagga aacagcctat ttccttgata ccatggaagt
     4321 tccccatatg cctatcgttc taacaggagc catgcgtagc tccaatgaac tcggtagtga
     4381 tggtgtttat aattacctaa gtgctttacg agtggccagc gatgacaggg ctgctgacaa
     4441 aggagttttg gtcgttatga acgatgaaat ccacgctgcc aagtatgtca ccaaaacaca
     4501 tacgactaat gtcagcacct tccagactcc aacacatggc ccccttggtc tcatcatgaa
     4561 acaggaaatc ctctacttca aaacagctga acctcgtgtt cgctttgacc ttgatcacat
     4621 acaaggttta gtccctatca tctcggctta tgctggtatg acagatgagc tgattgatat
     4681 gctggattta gaacacttgg acggtttgat tatccaagcc ttcggagctg gtaatattcc
     4741 caaagaaacg gctcaaaaat tagaaagcct tctgcaaaaa ggaattccag tcgctctggt
     4801 atcacgatgc tttaacggta ttgccgagcc tgtttatgcc taccagggtg ggggcgtaca
     4861 gttgcaaaaa gcaggcgttt tctttgttaa agaactcaac gcccaaaaag ctcgcttgaa
     4921 actcctcatc gccctcaatg ccggactaac aggacaggct ttgaaagact atatggaagg
     4981 ctaatactct tcgaaaatct ctgcaaacca cgtcacgtcg ccttaccgta tgtatggtta
     5041 ctgacttcgt cagtttcatc tacaacctca aaacagtgtg ttgcgcagcc tttaaccagc
     5101 cgcctagtcc gctctatggt attcattaag tcaacatctc ttgtttaaga gcacaaaatc
     5161 aggaaatctt ctcgattccc tgattttttc tatttacgtt ttcgtgttga gctacgttct
     5221 gtcaaaccat gaggtaagag aacttcacgt tcttccaact cttccttatg cataatcttg
     5281 gtcaacatac gcatactaat ggcaccaagg tcataaagag gttgggcaat cgttgtcaag
     5341 tttggacggg taaagcgtga gatttgtgaa tcatcactag taataatttc aaaatcttct
     5401 ggcacagaaa cacccttatc agccaaaccg ttcaagactc ctgctgccaa ctcatcacct
     5461 gtcacaactg ctgcagttgc atttgatgaa atcaaacgct ctgctaaggc gtaaccatca
     5521 tcatagctat atttagattc aaataccaaa ccctcactat aagtgattcc tgcttttttc
     5581 aaggtttcct tgtagccaac taaacgaacc ttaccattga tgtcatccac tagcggaccg
     5641 ctaacgaaag caatacgctc attttcttta acaaggtaac tcactgcatc aattgttgct
     5701 tgcttatagt caatattgac acttggcaac tggtgctcaa catcgacagt tcctgcgaga
     5761 acaatcggag tacgtgaacg cgaaaattct gagcgaattt tatctgtcaa gtgataaccc
     5821 atatagataa tgccatctac ctgctttgaa aagagggtat tgacaacaga aacttctttc
     5881 tcgttatctt catcgctatt agctaggaca atattgtact tgtacatttc tgcaatatca
     5941 tcaatcccct tagccaaact cgaaaaataa ccattggtaa tatttggaat cacgacaccg
     6001 acagtggttg tctttttact tgcaagacca cgcgcaactg catttggacg ataatccaaa
     6061 cgatcaatta cctctagcac ttttttacgg gtattctctt ttacattttt attgccattg
     6121 accacacggc tgaccgtcgc catggaaaca cctgcttcac gagcgacatc ataaatggtt
     6181 actgtatcat ctgcattcat tccttttcct gtcctttcta tctcacacat tcttttacaa
     6241 gtagaggtgc tgattgaagc tctatatcta cttacaaaag tgaagatgtg aaaatttcgt
     6301 tttcatattt ctacttattc cattctatca ctaattgtaa acactttcaa gtgttttttg
     6361 aagattgatt gaaaaaattt catagaaaac ctaggtttag ctccttgcta ccaccttaga
     6421 ctaaacaaaa aggaggaaac taagccctcc taaagttata gtaaaatgaa ataagaacag
     6481 gataaatcga tcaggacagt caaatcgatt tctaacaatg ttttagaagt agaggtgtac
     6541 tattctagtt tcaatctact ataggtattg ttccattcac taccgtcaat tttagcacat
     6601 agtcttcata aaatattata tcatcataac caaccagatt cttttgcgat attagctgcc
     6661 tctgttcgat taccagcatc cagttttgaa agaatattgg tgacatagtt tcgaatagtt
     6721 ccgtttgaga ggtaaagctg atctgcgatt tcttggttgg ataagccccg ggcgattccc
     6781 tttaagacag caatctcttg ttctgtcaac ggattgggac gggtcatcac cacttccatc
     6841 aattcaggcg aatactcctt gcgtccttcg aggacggtgt gcaaggtttg catgaggtct
     6901 gcaatgcttc gttcctttaa tacataagca tctactccag ccttgaccgc acgttcaaaa
     6961 taccctgggc gcttgaaggt cgtcactaca accacctttg tttcgagctt ttctgctcgt
     7021 atccactcca agacttcgag tcctgtctta acaggcattt ctacgtcaag gatggcgata
     7081 tctacagact ccttttctaa tagttgaatt gcttcttgac cattcttggc ttgtaagaca
     7141 gactctacat ctgcttgaaa ggcaagcaac tggcacatgg catctcgcaa catactttga
     7201 tcttctgcga ctaatacttt catctacttt ctctccttat aaggtagtcg aacttgcact
     7261 tccgttggat gtttctgact gattacactt acttctcctg aaaatggaga aacacgatct
     7321 cggactgtat ggagctcatc tccttttaga aaagcaaagc cgcagccatc atcacttact
     7381 gttaaaatga gttctttctc tgcccgttct aatttcaggt aggccttcga cgctttggca
     7441 tgtttgataa tattggtcac caactcaagc aaaatcatgg aagccgttga ctccaattct
     7501 tgagtaagac tagcagtatc tagttggtta tccgtttcca cctcaattcc agcaatttct
     7561 aacatctttt tcacagtctc tagttcggat gtcaaagttc tagacttaag attttccaca
     7621 atggttcgca cttcattcat ggaatcctta ctgatctggt gaatttcttt taattccttt
     7681 tccacctgtg gataagcctc catctgaaat aactgcaagg ctaaatctgt cttgacactc
     7741 aacatagcaa aggtatgtcc caagctatca tgcaaatcct gaccgatacg actgcgttca
     7801 ttttcagcaa gcaatagatt tatctgggca ttttgcttga cctgagcttc tttcaaatcc
     7861 tccacaatac gaatccgaac caagccaaaa gtcattaaat cgacaaaagc aagaattaca
     7921 agtagataga atagaaactc aacttcgatt ctctgaaaaa tcaacagttg gcccacaaca
     7981 aggacttgag caagaagaaa agtccagaca tgtaaagact ttaaaccacc tacgctgaaa
     8041 tgataactta agagattgga taggaaaaag aaaaaccaga tataattaac agcaacaaag
     8101 gcagtattcc caactacata agtcagcatg aggccccaat atagccaaga taggcgctgg
     8161 ctcttagttg ttaaaacacc caaatacgcc actacaaata gaatatcaat caataaatgc
     8221 cagctaggaa tctccccagt cactacaggt aggatgggga aaatcataaa aattaaactg
     8281 gcccaataca tatagtctat gctttttagt ctttcaagca ttaagcattc tccttatgac
     8341 cttgaagata aatggtcaaa ccaaacaaaa ctgctgaaaa aacaagtata taaactgtgg
     8401 ctgatagatt gatgccaccc tcgtttaaga aggttttgag caactccatc aactgataag
     8461 ttggtaggca cttcccgatt gcttgcatcc agtctggaaa taaagagatg ggcatccaga
     8521 gtccgcctaa aacagccaag cctagataga gaagattgcc cacgacagac atcaactgac
     8581 tagaaggcaa gagtgtcaag gtcaagccaa gcgctacaaa ggcaacactt cctactatta
     8641 gcaagaacgc agccccaatc cagcttccta gaggcatatc cacacctctt acaaaatgcc
     8701 caactgagaa aacaaccaga attgatacca aataatcaac catcatactt gttatctttg
     8761 atagataata ttctaccata tttacagggg tatggcgcaa tgttttctgc cagttgttga
     8821 ttttatcagt atgtaagaca gctgggaatg aaaacatagc tgtcgacatc atggaaaagg
     8881 ctgtcatgga gatgaggtaa tcacgcataa aattcgcgag ttcacctggt gtgtcctgat
     8941 agataccaga aaaaaataaa tagaaagccg tcggcatccc taccgacaat agataataga
     9001 ttaattgtcg tttggtcaat aaaaattcta tcttactaag tgctatccat cgtttcatct
     9061 tagttatctc ccttttgtgt ttcttcaaag attgtattca acaaactacg attattaact
     9121 tcgatttctt gcatcctaca tcctgcttgg gctaatagtt cccagaaagc atctgcttct
     9181 cgagtgacta cttgtagagc atcctgtttt tgtgaccagt tttcaaccaa gttagactgc
     9241 tcaatgactt ccttgtatgc cagaggaagg ataaaatgct tttcaattcc ctcactacgc
     9301 atagctagag gcgtcgtatc acgaatcaac tctcccttat ttaaaaccaa aatccggtca
     9361 gccgtatgct ctacctcttc aatataatga gacgaataga gaatcgtgac tccttgcgct
     9421 tttaggtccc gaacgatttc ccaaaagcgt tgacgagttg aagtatccat ggcagcagtt
     9481 ggttcatcta aaaagacaag ctttggtcgc ccaatcaagg tcaagacaaa agagaagaga
     9541 cgcttttgcc cgcctgacaa tttttctgcg aattgctctt tttgttgctg gtcaaactgc
     9601 aatagttgat cgatttcctg atcgctcaag gaatttggat agatacgttg aaagaaagca
     9661 atcaactctt tgacctttaa tttctgaacg atgacatttt cttgaggcag ataacctcta
     9721 atatagtcta actgagaact cgtcactgac aagccttgga tggatacttg accgcttgtg
     9781 accagtttat ctccaagcag acagtccaag agtgtggtct tcccagcacc attgggccca
     9841 atcaaggcga cgcattcacc ttcagctacc tcaaaggaaa tacccttcaa aatagccttg
     9901 cccttgatgt ttttatttag gctttctacc ttaatcatat tcatgatatt ctcctttcaa
     9961 ccactccatt ctcataagga aaacgacgaa aatcataaat ccaaacccca aagcaccacg
    10021 aatgaattgg cgaagcaagg tttggtcaaa ccaacctgta aacatttcca ctaaccatac
    10081 caagagtgac aggccgataa agaaatagat gatccctctc ttcattcctc aaactccttt
    10141 ttcacatctc cgactaattt caaaccttct ctaacaagcc aagacatcat tccaaagcca
    10201 gcaaagagct cccaaggaaa atgatagaaa ctctcatcca atcccgaaaa catgagttag
    10261 gtcataactc ctgctactac taaactcact gcgataatca ttttatttct catctcttct
    10321 tcctccattt catactacaa ttatagtctt ttgaaatcag aggagacaga agcttctgtc
    10381 actagaaaat atgacaaatg tcataaaaaa ttctgttcaa aacaagcaag atacactata
    10441 caataaaaca caatcagaaa aatctaaggc aacttcctca aaagagatat caaacccaat
    10501 tcacaccata atgtaaacta atacttattt aaaatcaaaa agagtagaaa tttttatcag
    10561 acaaacacat atatagtgta ttgaatctat aacagtaggg cttaaatact aacatatttc
    10621 tataaattaa tttaac
//
LOCUS       DAVLVF010000063        10451 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVLVF010000063 DAVLVF010000000
VERSION     DAVLVF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10451
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.063"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..1687
                     /locus_tag="VYN66_001517"
     CDS             <1..1687
                     /locus_tag="VYN66_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV1495521.1"
                     /translation="DKTNNEWFKEQTKAYIVEEKSTIKEVSDKQSIAGSPYSIGVYDR
                     LTSPSWKYPSMVLPLLTLPEKSVFIIANISTIGFGAYDRYRSKEHPAGTDLNDYVEKK
                     AKEAAVRFRDHYDYWYRILDDKNKEKLYRSVLVYDAFRFGTDEKEDKDTYQATFETNH
                     PAIKHFFGPAGNNVVHNSNGAYATGDAFYYMAYRMLDKDGAVTYTHEMTHNSDREIYL
                     GGYGRRNGLGPEFYAKGLLQAPDHPNDPTVTINSILKYDQSEESTRLQVADPTQRFGS
                     VDDLNKYMHNMFDVIYMLEILEGKAVAKLDTNQKYDLLRKIENEYKPDPDGNSVYATN
                     VVRRLKPEELTKLTTFNSLIEHDIITRRGYVDEATYKRNGYYTINLFSPIYSALSSKI
                     GTPGDLMGRRIAFELLAAKGYKDGMVPYISNQYEKEAKAQGKVITSYGKQIGLVTDEI
                     VLSKVFNNQYNSWIDFKKDMYKEREDKFGKLNKVSFIDPNGSWARQQKVTIDNINRLE
                     KMIEDAVKFDAEDEVAKLYPETNSRVLKLKKAIFKAYLDQTGDFRSSIFENKK"
     gene            1731..6944
                     /locus_tag="VYN66_001518"
     CDS             1731..6944
                     /locus_tag="VYN66_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1495522.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVG
                     TTGVDENGNLIEPPVNDIPEYTEPVGTTGVDENGNLIEPPVSDIPEYTEPISTVSEVA
                     SEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVRQVGEDGERQIVTSYEELHGK
                     KISDPVETVTILKEMKPKILVKGTKEKPKEKTAPVLTLDRTNTNVLNRSATLSYHLVN
                     TDGVTINKITATIKDGNEIVKTVDLTSEQLDKQVEDLKFYKDYKIETTMTYDRGKGEE
                     TATLEEKPLRLDLKKVEIKNIASTNLVKVNDDGTETPSDFMTEKPSDEDVKKMYLKIT
                     SRDNKVTRLAVDKIEEVTEEGKKLYKITAEAQDLIQHTDPTKVRNKYVHYIEKPVPKV
                     DDVYYNFKELVDAMNADKNGTFKIGADLNATNVPTPNKQYVPGTFKGHLSSVDGKQYT
                     IHNIARPLFDRVENGSVKNINLGNVDINMPWADGIAPVANMVKNATVEDVKVTGNVVA
                     NNNIAGIVNKIDSGGQLTNVAFIGNLTGVGDKGQYMAGIAGEIWRGNLAKAYVEADIV
                     ANRARIGGLVAKTDNGNDSMGIGKYGSIRKSVTKGTIKTKVLFETGGFINSNLPFGKL
                     EDNISMMRVENGEEFFGSSDLDYDGGYFTNGWLERNFVVKGVSSGKHSYKRSRDKIKE
                     ISQDEANKRIANFGLTADKYEINEPVVNRLNRLTRREDEYKSTQDYKSERDLAYRNIE
                     KLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGT
                     KEEMDVTKNTDSKVQQVREYSVSGLGDVVYTPNMVVKNRDKLIADVKSQLSSVELISQ
                     EVRDLMSRRDKPAENTDERKNGYIKDLYLEESFAEVKQNLDKLVKSLVENEDHQLNGD
                     EAAIKSLLKKVETNKAKIMMALTYLNRYYDIKYGDISIKNIMMFKPDFYGKTPSVIDR
                     LINIGSSEKNLKGDRTQDAYREIIAGNTGKSNLRNFLEYNMRLFTEDKDINDWFIHSA
                     KNVYVSEPKTTNTELKDKRHRVFDGLDNGVHGRMILPLLTLKDAHMFLISTYNTMAYS
                     SFEKYGKHTEEARNEFKTKIDEVAHAQQTYLDFWSRLALPNVRDRLLKSQNMVPTPVW
                     DNQTYNGSPVGRRGFDSKGNPIAPIRELYGPTWRHHDRDWRMGAMASIFPNPNNDDKV
                     LFMVTDMISPFGISAFTHETTHVNDRMLYFGGHKHRQGTDVEAYAQGMLQTPDSSTTN
                     GEYGALGINMAYHRPNDGNQWYNPDPDKLKTRDDIDRYMRNYNEAMMLLDHVEADAVL
                     PKIKGDNSKWFKKIDKEMRSKIQYNDLLGPNQWDSIRDLKDEEKVMTLSSVNDLVDNN
                     FMTKHGNPGNGRYRPEDFTPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGY
                     YGYENGFISYVSNKYKAEADKNNHGLLSDKLIINKVSKGNFNTLEEWKRHWYGEVLAK
                     AKKGFEAIDIDGVHISNYDELRPLFDKAVEEDLKKPDDFSHTVALKSKVFKALLKNTD
                     GFFNKLFKEDI"
     gene            7198..8049
                     /gene="ylqF"
                     /locus_tag="VYN66_001519"
     CDS             7198..8049
                     /gene="ylqF"
                     /locus_tag="VYN66_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV1495523.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            8036..8815
                     /locus_tag="VYN66_001520"
     CDS             8036..8815
                     /locus_tag="VYN66_001520"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV1495524.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            8831..10381
                     /locus_tag="VYN66_001521"
     CDS             8831..10381
                     /locus_tag="VYN66_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV1495525.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3572 a         1725 c         2137 g         3017 t
ORIGIN      
        1 agataaaaca aataatgagt ggtttaaaga acaaactaaa gcatatattg tagaagaaaa
       61 atctactatt aaagaggtaa gtgataagca atcaatagct ggtagtccat attcaattgg
      121 agtatatgat agattaacta gcccatcttg gaaatatcca agtatggtgt tgccgctatt
      181 aacattacct gaaaaatcgg tgtttattat agccaacatt tcgactatag gtttcggtgc
      241 ttatgataga tatagaagta aagaacatcc agcaggaact gatttaaatg actatgttga
      301 gaaaaaagct aaagaagcgg ctgttagatt tagagatcac tatgattatt ggtatagaat
      361 actagatgat aaaaataaag aaaaattata tagaagtgtt ctagtctatg atgcatttag
      421 atttggtaca gatgaaaaag aagataaaga tacatatcaa gcaacttttg aaactaatca
      481 tccagctata aaacatttct tcggtccagc gggaaataat gttgttcata attctaatgg
      541 agcatatgct acaggggatg cattctatta tatggcatat cgcatgcttg ataaagatgg
      601 tgcggtaact tatacacacg aaatgacaca taactcagat agagaaattt atcttggagg
      661 ctatggaaga cgaaatggac ttggaccaga gttttatgct aaaggactgc tacaagctcc
      721 tgatcatcct aatgatccaa cagtcactat taattccata ttaaaatatg atcaatcaga
      781 agaatcaaca aggcttcaag tagcagatcc aactcaacga tttggaagtg ttgatgatct
      841 aaataagtat atgcataata tgtttgatgt aatatacatg cttgaaatac tagaaggtaa
      901 agctgtagct aaattagata caaatcaaaa atatgattta ttaagaaaaa tagaaaatga
      961 atataaacct gatcctgatg gaaattctgt ttatgcaacg aatgttgtac gtagattaaa
     1021 accagaagaa cttactaaat taactacatt taacagtttg atagaacatg atattataac
     1081 aagaagaggt tatgtagatg aagcaactta taagcggaat ggctactata caattaatct
     1141 attctcacca atttattcag cattgagcag taaaatagga acaccaggag atttaatggg
     1201 acgacgtatt gcatttgagt tattggcagc caaggggtac aaagacggta tggttccata
     1261 tatttctaat cagtatgaaa aagaagcgaa agctcaagga aaagttatta catcatatgg
     1321 taaacaaata ggtttagtta ctgatgaaat tgttctttca aaagtattta ataaccagta
     1381 taattcttgg atagatttca agaaagatat gtataaagaa agagaagata aatttggaaa
     1441 gttaaataaa gttagtttta tcgatccaaa cggttcttgg gctcgacaac agaaagtgac
     1501 tattgataat attaatagac ttgaaaaaat gattgaagat gcagtaaaat ttgatgctga
     1561 ggatgaagtt gccaaattat atcctgagac aaatagtaga gttcttaaac tgaaaaaagc
     1621 aatctttaaa gcttatcttg accaaaccgg tgattttaga agttcaattt ttgagaataa
     1681 aaaatagtgt ttactattag gaaataaaaa gtgaaggtgg aggattagaa ttgtcattat
     1741 taaaaaaaga taaattttcc attcggaaaa taaagggtat tgttggttcg gtattcttgg
     1801 ggagtctttt atttgcaccg tcagttgttg gtgcatcgac ttatcattac ttagattata
     1861 gtagtttgac acaaactgaa cgtgatcaac tcaaacaagg tagacctgat gaatccaaag
     1921 agtcatatgc tttggattat gaaaaagacg cactaccaaa cacaggaagt tctcaatcta
     1981 ttatgactgc attgggtttg ttagctatcg ggagtcttat tgtgattatc acaaaagata
     2041 atagaaataa aaaaatagct acatttttga ttgtaggggc gacaggttta gttacactat
     2101 caactgcttc agcacttaat ttgaatgcta acatccatga gtctggacgt gatggtgtgt
     2161 tgcaaatttc tggttacaga tatgttggtt acttggagct tgatgataaa actgtttcgt
     2221 cggtttctcc agcatcaact gtttcgccag ttgagcaacc caaagttgta acagagaaag
     2281 gcgaaccgga agttcatgag aagcctgact atacgcaacc aataggtgca aaccttgttg
     2341 agcctgaagt tcatgagaaa ctagcataca ctgaaccagt agggacaaca ggagtagatg
     2401 aaaatggaaa cttgattgaa ccacctgtta acgacatccc agaatatact gagccagtag
     2461 gtacaacagg agtggatgaa aatggaaact tgattgagcc acctgttagc gacattccag
     2521 agtatactga accaatatct acagtttcag aagttgcatc agaaagagaa gagttgcctt
     2581 ctttacatac cgatattcgg acagagacta ttcctaaaac cactatagaa gaatctgatc
     2641 ctagcaaatt tataggcgat gattctgtta gacaagttgg tgaggatggc gaacgtcaaa
     2701 ttgttactag ctatgaagag ttacatggca aaaaaatcag tgacccagtt gaaacagtaa
     2761 ctattttgaa agaaatgaag cctaaaattc ttgtaaaagg taccaaagaa aaacctaaag
     2821 aaaaaacggc tcctgttttg accttagata gaacaaatac aaatgtatta aaccgatccg
     2881 caactctttc ttatcattta gttaatacag atggagtcac aatcaataaa attactgcga
     2941 caattaaaga tggaaatgaa attgtcaaaa cagtagattt gacttcagag caattagata
     3001 agcaggtaga agatttaaaa ttctacaagg actataagat agagaccact atgacctatg
     3061 accgtggcaa gggagaagaa actgcaacat tagaagagaa accacttcgc ttggatttga
     3121 aaaaagttga aattaaaaat atcgcatcta ctaatttagt gaaggtaaat gacgatggta
     3181 ctgaaacacc tagtgatttt atgactgaaa aaccttcaga tgaagatgtg aagaaaatgt
     3241 acttaaaaat tactagtcgt gataataaag taacacgttt agcagttgat aaaattgaag
     3301 aagtgactga agaaggtaag aaactttata aaatcactgc cgaagctcaa gatttgattc
     3361 aacatacaga cccaacgaag gttcgtaaca agtatgttca ttatattgaa aaaccagttc
     3421 caaaagttga tgacgtttat tataacttta aagagttagt agatgccatg aatgctgata
     3481 aaaatggtac atttaaaatc ggagcagatt tgaatgcaac gaatgtacca acacctaaca
     3541 aacaatatgt tccaggtacg tttaagggac atctatcaag tgttgatggt aaacaatata
     3601 caattcataa cattgcccgt cccttatttg accgtgttga aaatggttct gtgaaaaata
     3661 ttaatttagg taatgtggat atcaatatgc cttgggctga cggaatagca cctgttgcca
     3721 atatggtgaa aaatgcaact gttgaagatg ttaaggtaac tggtaatgta gttgctaata
     3781 acaatattgc gggtatcgtc aataaaatcg atagtggtgg tcagttgaca aatgtagcct
     3841 tcatcggtaa tcttacaggt gttggtgata aaggtcaata tatggctggc attgccggtg
     3901 agatttggag aggtaatctt gcaaaagcat atgtcgaggc tgatattgtt gctaacagag
     3961 cccgtatagg tggtttagtt gctaaaacag ataatggtaa cgactctatg ggaattggta
     4021 aatacggttc aattcgtaaa tcagtcacca aaggaaccat taagactaaa gttttgtttg
     4081 aaacaggtgg atttatcaat agcaatcttc cttttggtaa acttgaagat aatatttcaa
     4141 tgatgagagt tgaaaatggt gaagagttct ttggttcttc cgatttggac tacgatggtg
     4201 gttatttcac aaatggctgg ttagagcgta actttgttgt caaaggtgtc agttcaggta
     4261 agcattcata caaacgttct cgtgacaaaa ttaaagaaat tagtcaagat gaagctaata
     4321 agagaattgc aaattttgga cttactgctg ataaatacga aatcaacgaa cctgtggtca
     4381 atcgcttaaa tcgtttaacc agaagagaag atgaatataa gtcaactcaa gactacaaat
     4441 ccgaacgtga tttagcttat cgtaatattg agaaacttca accattttat aataaagaat
     4501 ggattgtcaa ccaaggtaac aaacttgcag aagattcaaa cttagctaag aaagaagtcc
     4561 tttctgttac ggggatgaag gatggtcaat tcgtgaccga tttatcagat attgataaga
     4621 ttatggttca ctatgctgat ggtactaagg aagaaatgga tgttactaag aatactgatt
     4681 ctaaagtcca acaagtgcgt gaatatagtg tatctggtct tggtgatgtt gtttatacac
     4741 cgaatatggt tgttaaaaat cgtgataaat tgattgctga tgttaaatct caattatcaa
     4801 gtgtggaatt gatttcacaa gaagtaagag atttaatgag tagacgtgat aaacctgcag
     4861 agaatacgga tgaacgtaag aatggttata ttaaagatct atacttagaa gaaagctttg
     4921 ccgaagtgaa gcaaaatcta gataagttag tcaaatcctt ggtcgaaaat gaagaccatc
     4981 aattgaatgg tgatgaagct gcgataaaat cacttctcaa gaaagtggaa accaataaag
     5041 ctaaaatcat gatggctctg acttatctca atcgttacta tgatatcaaa tacggtgaca
     5101 taagtataaa aaacatcatg atgttcaaac ctgattttta tggtaaaaca ccaagtgtta
     5161 ttgacagact catcaacatc ggttcaagtg aaaagaactt gaaaggggat cggactcagg
     5221 atgcttaccg tgaaatcatc gcaggtaata ctggtaaaag taatttgcgt aacttcttag
     5281 aatacaacat gcgtctgttt acagaggata aagacatcaa tgattggttt atccactcag
     5341 ctaagaatgt ttatgtttca gaacctaaga caacaaatac agagttgaaa gataaacgtc
     5401 atcgtgtgtt cgatggttta gataacggtg ttcatggtcg tatgatttta ccacttctaa
     5461 cgcttaaaga tgcccatatg ttcttaattt caacatataa cacgatggcg tacagttcat
     5521 ttgagaaata tggaaaacat acagaagaag ctcgtaatga gttcaaaact aagattgatg
     5581 aagttgcaca tgctcaacaa acttatttag atttctggtc cagattagct ctgcctaatg
     5641 ttcgtgatag acttcttaag agtcaaaata tggtaccgac accagtttgg gataaccaga
     5701 cctacaatgg ttctcctgta ggtcgtcgag gatttgacag taaaggtaac cctatagctc
     5761 caattcgtga attatatggt ccaacatggc gacaccatga tagagactgg cgtatgggcg
     5821 ctatggcatc aatattccct aatccaaata atgatgacaa ggtcttgttt atggtgacgg
     5881 atatgattag cccatttggt atttcagcct ttactcacga aacaacacac gtaaacgata
     5941 gaatgcttta cttcggtggt cataagcatc gtcaaggtac agacgttgaa gcctatgctc
     6001 aaggtatgtt gcaaacacct gacagctcaa cgactaatgg tgaatacggc gcacttggaa
     6061 ttaacatggc ttaccatcgt ccaaatgatg gtaaccaatg gtacaaccca gatcctgaca
     6121 aattaaagac acgtgatgac attgaccgct acatgagaaa ctacaatgaa gctatgatgt
     6181 tgcttgatca tgtagaagct gatgctgtac ttcctaaaat taaaggagac aatagtaagt
     6241 ggttcaagaa gattgacaaa gaaatgcgct ctaagattca gtataatgat cttttaggtc
     6301 caaaccaatg ggatagtatc cgtgatttga aagatgaaga aaaggtaatg actttatcta
     6361 gcgtcaatga tttagtggat aataacttca tgacaaaaca tggaaatcca ggtaatggac
     6421 gttatagacc agaagatttt acaccaaact cagcttacgt aaatgtcaat atgatggcag
     6481 gaatttacgg tgggaatacg agtcaaggtg ctcctggttc attatcattt aagcacaatg
     6541 ctttccgtat gtggggctat tatggttatg aaaatggctt tattagttat gtatcaaata
     6601 aatacaaagc agaagcagat aaaaacaacc acggtttact aagcgataag ctaataatta
     6661 ataaagtatc gaaaggaaac tttaatactc ttgaagagtg gaagagacat tggtacgggg
     6721 aagttcttgc taaagctaag aaaggttttg aggctatcga tattgatggc gtccatatca
     6781 gtaactatga tgagttaagg cctttatttg ataaagcagt tgaagaagat ttgaagaaac
     6841 cagatgactt ctctcacact gttgctctta aatctaaggt cttcaaagcc cttcttaaaa
     6901 acacagacgg tttctttaat aaactgttta aagaagacat ttaaatgttt agaatataga
     6961 aggaacagtt ttttgctccc tctgaaaagt catcatttga tggctttttt ctatataggg
     7021 tacttgaaag gctatcatcg gacaaaataa agaaggcatg atataatata aggtagattt
     7081 ctatgccata aaacaaaaaa ctgtttgaac atcattcatt tgagaaccct ttatgttcaa
     7141 acgatagtaa aataaaatag gatctctaaa cccttgctac gaaaggaaaa aaactcaatg
     7201 gctactattc aatggtttcc tggtcacatg tctaaagcgc gtcgacaggt gcaggaaaat
     7261 ttaaaatttg ttgattttgt aacgattttg gtggatgcac gcttacctct atctagtcaa
     7321 aatcctatgt tgaccaagat tgttggtgat aaaccaaaac tcttgatttt aaataaggcg
     7381 gatttggctg atccagcaat gaccaaggaa tggcgtcagt attttgaatc acaaggaatc
     7441 caaactctgg ctatcaactc caaggagcaa gtgactgtaa aagttgtaac agatgcggcc
     7501 aagaagctca tggctgataa gattgctcgc cagaaagaac gtgggattca gattgaaacc
     7561 ttgcgtacta tgattatcgg gattccaaac gctggtaaat caactctgat gaaccgtttg
     7621 gctggtaaaa agattgctgt tgttggaaac aagccagggg tcacaaaagg tcaacaatgg
     7681 cttaaaacca ataaagacct ggaaatcttg gatacaccgg ggattctctg gcctaagttt
     7741 gaggatgaaa ctgttgcact taagttggca ttgactggag ctatcaagga tcagttgctt
     7801 cctatggatg aggttaccat ttttggtatc aattatttca aagaacatta tccagaaaag
     7861 ctggctgaac gcttcaaaca aatgaaaatt gaagaagaag cgcctgtgat tattatggat
     7921 atgacccgcg ccctcggttt ccgtgatgac tatgaccgtt tttacagtct cttcgtgaag
     7981 gaagtccgtg atggcaaact cggtaactat accttagata cattggaaga cctcgatggc
     8041 aacgattaaa gaaatcaaag aattccttgt gacagtcaag gagttagaaa gccctatttt
     8101 tttagagctt gaaaaggata atcgctcagg agttcaaaag gaaatcagca agcgtaaaag
     8161 agccattcaa gctgaattag atgaaaattt gcgcttggaa tccatgcttt cttatgaaaa
     8221 agaactttat aagcaaggat tgaccttaat tgtaggtatt gatgaggttg gtcgtggtcc
     8281 tcttgctggt cctgtagtcg ctgcggccgt cattttacct aaaaattgta agattaaagg
     8341 tctcaacgac agcaagaaaa ttcctaaaaa gaaacatctg gagattttcc aagccgttca
     8401 agaccaagcc ttgtcgattg gaattggtat catagataat caggtcatcg accaagtcaa
     8461 catctatgaa gcaaccaaac tagccatgca agaagcaatc tcccagctca gccctcaacc
     8521 agagcacctt ttgattgatg ccatgaaact ggacttgccc atttcacaaa cctccattat
     8581 caaaggagat gccaactccc tctctatcgc agcagcatct atagtagcca aggtaacacg
     8641 tgatgaattg atgaaggaat acgatcagca gttccctggc tatgatttcg ctactaatgc
     8701 aggatatggc acagctaaac atctggaagg cctcacaaaa ctaggagtta ccccaattca
     8761 ccgaaccagc tttgaacccg ttaaatcact ggttttaggt aaaaaagaaa gttaattgaa
     8821 aggaaatacc atggaggaac agtcggaaat agtccgttct aagaaagaat tcgcctttgc
     8881 atccagcact atactatccc aagttggtcg aggaatcatt gtcggcctca tcgttggaat
     8941 tatcgtcgga tcctttcgtt tcttaattga aaagggcttc cacctgatac aaggagttta
     9001 tcaagatcaa gggtacttag tgcgcaatct ttttgtactg gttttgtttt atatactcat
     9061 ctgttggctc agtgccaaac taacacggtc agaaaaagat attaaaggct caggaattcc
     9121 tcaagtcgaa gccgaactga aaggcctcat gtccctcaac tggtggggca ttctttggaa
     9181 aaaatatgtg ctaggtattc ttgctattgc cagtggactc atgctgggtc gagagggacc
     9241 cagtattcaa cttggagcag ttggtggtaa aggaattgcc aagtggctca aatccagtcc
     9301 agtagaggaa cgttccttga ttgccagtgg agctgcagca ggtttagccg cagcctttaa
     9361 tgctcctatt gcagcacttc tctttgttgt agaagaagtc tatcaccatt tttcgcgctt
     9421 tttctgggtc tcaactctag cagccagcat cgtagcaaac tttgtgtctc tactcatgtt
     9481 cggtttgaca ccagtattgg atatgccaga taacattcct cccatgaccc tagatcagta
     9541 ttggatatat ctcgtcatgg gaattttcct tggattttca ggttttctct atgagaaagc
     9601 tgtattaaac gttggaagag tttatgactt gattggtcaa aaaatccatt tggatagggc
     9661 ttattatccc atcttggctt ttatccttat cataccagtc ggaatcttct tacctcaaat
     9721 cattggtggc ggaaatcagc ttgtcctttc tttaactgaa caaaatttta gtttccaagt
     9781 tttattagct tactttttaa tccgctttat ttggagtatg attagctatg gaagtggact
     9841 gccaggagga attttcctcc ccattttagc tcttggttct ttgcttggtg ccttagttgg
     9901 tgttatctgt gtcaatcttg gacttgtcag tcaagagcaa ttccctatat ttgtcattct
     9961 aggaatgagt ggctattttg gagccatatc aaaagctccc ttaaccgcta tgatcctcgt
    10021 aactgagatg gtaggagata ttcgcaacct tatgccactt ggtcttgtca ctcttgtttc
    10081 ttatattatc atggatttgc tcaaaggtac gccagtctat gaagccatgc tggaaaaaat
    10141 gcttccagaa gaagtatcta gcgaaggaga agttacactt atcgaaatac cagtttctga
    10201 taaaattgct gggaaacaag ttcatgaact caacttacca cacaacgtcc tcatcacaac
    10261 tcaagtccat aatggcaaga gccaaacagt taacggctca accagaatgt atctgggtga
    10321 tatgattcac ctggttattc caaaaagtga aattggaaaa gtcaaagatt tgttgttgta
    10381 gtatgatcat ttacataatt tatgttatgt aaatgatcag tttgatttat ttagaaaacc
    10441 gattctcaga a
//
LOCUS       DAVLVF010000064        10254 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVLVF010000064 DAVLVF010000000
VERSION     DAVLVF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.064"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            85..1260
                     /gene="mutY"
                     /locus_tag="VYN66_001522"
     CDS             85..1260
                     /gene="mutY"
                     /locus_tag="VYN66_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV1495526.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1315..2019
                     /locus_tag="VYN66_001523"
     CDS             1315..2019
                     /locus_tag="VYN66_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1495527.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2012..3361
                     /gene="vicK"
                     /locus_tag="VYN66_001524"
     CDS             2012..3361
                     /gene="vicK"
                     /locus_tag="VYN66_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV1495528.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3363..4172
                     /locus_tag="VYN66_001525"
     CDS             3363..4172
                     /locus_tag="VYN66_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1495529.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4263..4505
                     /locus_tag="VYN66_001526"
     CDS             4263..4505
                     /locus_tag="VYN66_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV1495530.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4507..4761
                     /locus_tag="VYN66_001527"
     CDS             4507..4761
                     /locus_tag="VYN66_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1495531.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEGPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4808..5503
                     /locus_tag="VYN66_001528"
     CDS             4808..5503
                     /locus_tag="VYN66_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV1495532.1"
                     /translation="MKKKWLFADYYDTTIILLALISVILVLLGFAEMTDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLTILPLNAIFTVFRLGRIFRLARLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPVSLFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSS"
     gene            complement(5852..6838)
                     /locus_tag="VYN66_001529"
     CDS             complement(5852..6838)
                     /locus_tag="VYN66_001529"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV1495533.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            7032..9500
                     /gene="gyrA"
                     /locus_tag="VYN66_001530"
     CDS             7032..9500
                     /gene="gyrA"
                     /locus_tag="VYN66_001530"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV1495534.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINAESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLTEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            9500..10243
                     /gene="srtA"
                     /locus_tag="VYN66_001531"
     CDS             9500..10243
                     /gene="srtA"
                     /locus_tag="VYN66_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV1495535.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         3180 a         1747 c         2288 g         3039 t
ORIGIN      
        1 gatagaaaag ttatatacta tctattgaaa atattataaa tcagatacaa agacaggtga
       61 aaccttctgt gttatactag agatatgtta gatttgaaag aatacggtat cgtcatgtgg
      121 ccggaggaga aagtcatttc tttccgtgag aaacttctcg cttggtatga tgaaaacaaa
      181 agagatttgc cttggaggag aagtaaaaat ccttatcaca tctgggtatc tgaaatcatg
      241 cttcagcaga ccagggtgga tacagttatc ccttactacg aaagattcct ggactggttt
      301 ccaactgtcg aaagtctggc aactgcgcct gaggagagtt tactgaaagc ttgggagggc
      361 ttgggctatt attctcgagt tcgcaatatg caggctgcag cccagcagat tatgactgac
      421 tttggtggcc aatttccaaa tacctatgaa ggaatttcca gcttgaaagg gattggacct
      481 tacacagcag gagccatttc cagtattgct tttaacttgc ctgagccagc tgtagatggt
      541 aatgtcatgc gggtcttggc gcgtctgttt gaagtcaacc acgatattgg gattccaagt
      601 aatcgaaaaa tttttcaggc aatgatggag atcttgatta atccggatcg tccgggtgat
      661 tttaatcaag ccttgatgga cttagggtct gatattgagt ctcctgtaaa tcccagacct
      721 gaagaaagcc cagtcaagga ctttagtgcg gcatatcaga atggaaccat ggaccgttat
      781 ccaattaaat ctcctaagaa aaagccggtt cctatttatc ttaaagcctt agttgtgaaa
      841 aattctcaag gacaattttt acttgaaaaa aatgaaagcg aaaagctctt ggcaggtttt
      901 tggcatttcc cctttataga agttgataac ttttcgcaag aagagcagtt tgacctcttt
      961 catcaggttg cggaagaaag tgtgaacttt ggccccagtc cagaagagag tttccagcag
     1021 gactatgacc tagatgttga ttggcttgat gtttgttttg atacagtcca gcatgttttt
     1081 agtcatcgaa aatggcatgt acagattgta gcaggtcagg tgagtgactt ccatgatttt
     1141 tcagataggg aagttcgttg gctttcacca gaagaattca agaattaccc acttgccaaa
     1201 ccccaacaaa aaatctggca ggcttatgca caagccaact tagacagtag ccaagactag
     1261 ctattgtgtc ttctgactat tttttggtat aatagtagag aaaaaggtga acatatgaaa
     1321 aaaatactaa ttgtagatga tgagaaacca atctcggata ttatcaagtt taatatgacc
     1381 aaggaaggtt acgaagttgt aactgctttt aatggtcgtg aagcgctaga gcaatttgaa
     1441 gcagagcaac cagatattat tattctggat ttgatgcttc cagaaattga tggtttagaa
     1501 gttgctaaga ccattcgtaa gacaagcagt gtgcccattc ttatgctttc agccaaagat
     1561 agtgaatttg ataaggttat cggtttggaa cttggggcag atgactatgt aacgaaaccc
     1621 ttctccaatc gtgagttgca ggcgcgtgtt aaagctcttc tgcgtcgttc tcaacctatg
     1681 ccagtagatg gtcaggaagc agatagtaaa cctcaaccta tccaaattgg ggatttagaa
     1741 attgttccag acgcctacgt ggctaaaaaa tatggcgaag aactagactt aacccatcgt
     1801 gaatttgagc ttttgtatca tttagcatcg catacaggtc aagtcatcac gcgcgaacac
     1861 ttgcttgaga ctgtctgggg ttatgactat tttggtgatg tccgtacagt tgatgtgact
     1921 gtacgacgtc tgcgtgagaa gattgaagat acgcccagcc gaccagagta tatcttgacg
     1981 cgccgtggtg tagggtatta catgagaaat aatgcttgat ttactgaaac aaaccatttt
     2041 taccagagat tttatcttta tcctgatttt gttaggtttc atccttgttg tgaccctctt
     2101 attactggaa aatagacgtg ataatattca gttgaagcaa atcaatcaaa aggttaaaga
     2161 tttgattgca ggagattatt ccaaggttct tgatatgcaa ggtgggtctg aaatcaccaa
     2221 tattaccaat aatttgaatg acttgtcgga ggttattcgt ctcactcagg aaaatctaga
     2281 acaagagagt aagaggctaa atagtattct gttttatatg acagatgggg ttcttgcgac
     2341 caaccgtcgg ggtcagatta tcatgattaa cgatacagcc aagaagcaac tggggttggt
     2401 taaggaagat gttctgaata gaagcatttt ggaattgctc aagatagaag aaaactatga
     2461 attgcgtgat ttgattaccc aaagtccaga attgttgcta gattcccaag atatcaatgg
     2521 cgaatatttg aaccttcgag ttcgctttgc cttgatacgt cgagagtctg gctttatttc
     2581 aggtttggtg gctgttttgc atgatacgac ggagcaggag aaggaagaac gcgaacgaag
     2641 actctttgtt tccaatgtta gccatgagtt acggactcct ctgactagcg taaaatccta
     2701 tcttgaagcc ttggatgagg gggctttgtg tgaaactgta gcaccagact ttatcaaggt
     2761 ttctcttgat gagaccaacc gtatgatgcg catggtgacg gatctcctcc atctttcacg
     2821 tattgataat gctaccagtc acctagatgt ggaactgatt aacttcactg cttttattac
     2881 ctttatcctc aatcgttttg acaagatgaa aggacaggaa aaggagaaaa aatatgagtt
     2941 ggtgagagat tatcccatca attctatctg gatggaaatt gatacagata agatgacgca
     3001 ggttgtcgac aatattttaa ataatgctat taagtattcg ccagatgggg gtaaaatcac
     3061 tgtcagaatg aagacaactg aagaccagat gattttatcc atttctgacc acggtttggg
     3121 gattcctaag caggatttac cacgtatctt tgaccgtttc tatcgtgtgg atcgtgctag
     3181 aagtcgtgca caaggtggta caggtctagg actgtctatc gctaaagaaa ttatcaaaca
     3241 acataagggc tttatttggg ccaagagtga atacggcaag ggttcaacct ttaccattgt
     3301 gctcccttat gataaggatg cagtgaaaga agaagtatgg gaggatgaag tagaagacta
     3361 gaatgagtga aataggcttt aaatacagta ttttagcgtc gggttccagt ggaaattctt
     3421 tttatctgga aacctcaaaa aagaagcttt tagtagatgc aggcttgtct ggcaagaaaa
     3481 ttaccagtct gctagctgaa attaaccgta agccagaaga cctggatgcc atcttgatta
     3541 cccatgagca ttcagatcat atccatggag taggcgtttt ggctcgcaag tatggtatgg
     3601 atctttatgc caatgaaaag acctggcaag ctatggaaaa tagtaaatat cttggcaagg
     3661 tggattcttc gcaaaagcat atttttgaaa tgggtaaaac caaaaccttt ggagatatcg
     3721 acatcgagag ttttggtgta agccatgatg cagtcgcacc gcagttctat cgctttatga
     3781 aggatgataa gagttttgtc cttttgacag atacaggtta tgtcagtgac cgtatggcgg
     3841 gcattgtcga aaatgcggat ggctatctta tcgaggccaa ccatgatgta gagattttgc
     3901 gatcaggttc ttacgcttgg cgactcaaac aacgaatcct atctgacctt ggtcaccttt
     3961 ctaacgagga cggtgctgaa gctatgattc ggacgctagg aaatcgtact aagaagattt
     4021 accttgggca tttatctaag gaaaataata tcaaggaact ggctcatatg accatggtca
     4081 atcagctggc tcaagctgat ctgggagtcg gagtagactt taaggtttat gatacctcac
     4141 cagataccgc aacaccattg acagagatat agaaagaacg ctgagaaggt gttcttttta
     4201 tattgactga acacctaaaa agtaatacaa tggtgttacc attaaaaaag ggagcacaaa
     4261 agatgactac tattacatta aaagtttctg aagctgataa aacatttatg aaagcaatgg
     4321 ctaagtttga aggagtttcc ctttcggaac ttattcgcac caaaactctt gaagccctag
     4381 aagatgaata cgatgctcgt gtggcagatt tagcctatca agagtattta gaagacttgg
     4441 aaaaaggagt tgaacccatt acttgggaag aaatgatgca tgatttaggc ttgaaggatg
     4501 aataatttgt ataaattagt tccaacaaga cgttttatca agcaattgaa aaaattggac
     4561 cgttatacgc agaagctaat tacaaactat ttacaaacca atgttttgga aggtccaaga
     4621 cgacacggaa aggctttggt tggtaatcgc gttggtcaat ggcgctatag aattggtaat
     4681 tatcgagtta tcgtacaaat tgtagatgat gaattagtcg ttgctactct agaagttggt
     4741 catcggagag atatttattg aattaaagct gagtaataac aatatagaaa gtaaggagag
     4801 taaaaatttg aagaaaaaat ggttatttgc agattattat gatacaacaa ttattttact
     4861 tgctttaatc tctgttattc tggtattact tggatttgct gagatgactg atttggacaa
     4921 tccgccttat agtattattg atttagtaat ctggggtgtt tttgtaattg attatagttg
     4981 gcgctttttt attactaaaa gaaaatggcg ctttattctt gaaaatattt ttgatttatt
     5041 gaccattctc cctttaaatg ctatttttac agtgtttcga ttaggacgta tcttccgctt
     5101 agcaaggctg acaaaattac tgaaactgac tcgtttactt agaataattg gacttacagg
     5161 taaactagaa agaaaaatta gtagattctt acgaacaaat ggattgattt atatcttgta
     5221 tgttaatatc tttattgttc tagtagggag ttcgatttta tccgttgttg aagaaaaatc
     5281 cttctcagat agtctttggt gggctcttgt gacagtaact actgttggtt atggtgatat
     5341 tgttccagtt tccctttttg ggaaatggct agcagtttta ctgatgcttg tcggtattgg
     5401 aacaataggg atgctaacaa gtgctttgac gaactttttt gtaaaggata atccagatga
     5461 acagataaaa cttgataaac tcaaagacga attatcgagc taaagaatat taatagagaa
     5521 acaatctgaa aaaatcgaag aactacattg aatgataaaa gacttagttg aaaaaatatc
     5581 ataaaggtga gacaaaataa aacttgattc gaggagaaat caagtaagtt ttgacacaat
     5641 agaacgcata ttatcaggta taaaaacttg atactatgcg ttttatagag aaataaaata
     5701 aaatctgaac aaatcaatta ggaatttaaa gcattttata acaacgtttt agagtaatgg
     5761 ggtgctattt caacttcaac ctactataat acagaaaaaa atacagaaaa aaacaactcc
     5821 ctgatgattc aaggagttgt ctatagttaa attagttttt agaagcttct tggaattctg
     5881 gatttttcca tgcttcgtca ataatagctt gcaattcttt agcagatgct tgcatttttt
     5941 gagtttctgc atcgttcaat ggaatgttta ctggacgaac gataccatgt gcaccaacaa
     6001 cagctggttg accgataaag acattctcaa ctccgtattg accttcttgg aatactgaaa
     6061 gtggaagtac tgcgttttca tcgtcaagga ttgctttagt gatacgagca agggctactg
     6121 cgataccgta gtatgttgca ccttttttgt tgatgattgt gtaggctgca tcacgaacac
     6181 cttcgaacaa ttcaatcaat tcagcttctt gaacattttg agtgtcttta aggaattctt
     6241 caaggtttac accagcgatg ttagcgtgtg accaaacagc gaactcagag tcaccgtgtt
     6301 cacccatgat gtaggcgtgc actgaacgag catccacatc caatttttca gcaagtgctt
     6361 gacggaaacg agctgagtca agtgaagtac ctgaaccgat aacgcgttct ttagggaaac
     6421 cagagaattt ccaagttgag taagtcaaaa cgtcaactgg gttagcagca acaaggaaga
     6481 tacctttgaa accagattca acaacttgag ttacgattga tttgttgata gcaaggtttt
     6541 tacctacaag gtcaagacga gtttcacctg gtttttgagg tgcacctgca gtgatcacaa
     6601 caaggtcagc gtctgcacag tcagagtatt gagctgcata gattttttta ggtgaagtga
     6661 aggcaagggc gtgactaagg tcaagcgcat caccaacagc tttttcatgc aattgtggaa
     6721 tttcgataat tccaagctct tgtgcaattc cttggttaac aagtgcaaaa gcgtaagatg
     6781 aacctacagc accatcaccg acaaggataa cttttttgtg ttgtttagtt gaagtcattg
     6841 ttttaaacat ctccttaatt ttattagggg attttcccta gacaacttca ttctatcact
     6901 tttaaaaaac tttgtcacga atatgcctta tagttctcga tgtaaacgtt ttagtggttt
     6961 agaggctgaa atagatggga atttatggta taatgttgtt acttactaat tgtgaaatga
     7021 ggcatttatt aatgcaggat aaaaatttag tgaatgtcaa tctgacaaag gagatgaagg
     7081 caagttttat cgactacgcc atgagtgtta tcgtagcgcg agctcttcct gatgttcgag
     7141 atggcttaaa acctgttcac cgtcgcattc tctacggaat gaatgaattg ggtgtgaccc
     7201 cagacaaacc ccataaaaaa tctgctcgta ttacagggga tgtcatgggt aaatatcacc
     7261 cacacgggga ttcctctatt tatgaagcca tggtccgtat ggctcaatgg tggagctacc
     7321 gttacatgct tgtagatggt catgggaatt ttggttccat ggatggagat agtgctgccg
     7381 ctcaacgtta taccgaggca cgtatgagca agattgctct ggaaatgctt cgtgatatca
     7441 acaaaaatac agttgatttc gttgataact atgatgccaa tgaacgggaa cccttggtct
     7501 tgccagcgcg ttttccaaac cttttggtta atggagcaac tggtatcgcg gttgggatgg
     7561 caaccaatat tccacctcat aatctgggtg aaaccattga tgcagtgaag ttggtcatgg
     7621 ataatcctga agtgactacc aaggacttga tggaagtctt gcctggacca gattttccaa
     7681 ctggtgctct tgtcatgggg aaatcaggta tccataaggc ttatgaaaca ggtaaaggtt
     7741 cgattgtcct acgttctcgt acagagattg aaacgactaa gactggtcgt gagcgtatcg
     7801 ttgtaacaga atttccttac atggtcaata aaaccaaggt gcatgagcat attgttcgct
     7861 tggttcagga aaaacgcatt gagggtatca cagcagtacg tgatgagtca aaccgtgaag
     7921 gtgttcgatt tgttattgaa gtcaagcgcg acgcctcagc caatgttatt ctcaataacc
     7981 tcttcaaaat gacccaaatg caaaccaatt ttggattcaa tatgctcgct atccaaaatg
     8041 gtataccgaa aattttgtct cttcgtcaga ttttggatgc ttatattgag caccaaaaag
     8101 aagtggttgt tcgtcgtaca cgttttgata aggaaaaagc ggaagcgcgc gctcatatct
     8161 tagaaggtct cttgattgcg ctagaccata tcgacgaagt gattcgtatc atccgtgcta
     8221 gtgaaacgga tgcggaagct caagctgagt tgatgagcaa gtttaagctt tctgaacgtc
     8281 aaagtcaagc tatccttgat atgcgtcttc gtcgtttgac aggtttggaa cgcgataaga
     8341 ttcaatctga gtatgatgac ctcttggctc tgattgcgga tttagcagat attcttgcta
     8401 agcctgaacg tgtttctcaa attatcaaag acgaattgga tgaagttaaa cgtaaatttt
     8461 ctgataaacg ccgtacagag ttgatggttg gacagatctt gagtctcgag gatgaggact
     8521 tgattgaaga atcggatgtc ttgattaccc tttctaacag aggctacatt aagcgtttgg
     8581 atcaggacga gttcactgct caaaaacgtg ggggtcgtgg tgtccaagga acgggagtga
     8641 aagatgatga ctttgttcgt gagttagtgt caactagcac ccatgatcat ctgctcttct
     8701 tcacaaacaa gggacgtgtc tatcgtctta aaggttatga aattcctgag tatggtcgga
     8761 ctgccaaagg gctaccagta gtcaatctct tgaaattgga tgaagatgaa agtattcaga
     8821 cggttatcaa tgctgagtct gatcgcagtg atgatgctta tctcttcttt acaacccgtc
     8881 acggtattgt gaagagaact agtgttaagg agtttgccaa tattcgtcaa aatggtctca
     8941 aagcgctgaa tttaaaggat gaagatgagt taatcaatgt cttgttgaca gaaggagata
     9001 tggatattat cattggtacc aagtttggtt atgcagttcg ctttaatcaa tcagccgttc
     9061 gtggtatgag ccgtatcgcc actggtgtga aaggtgttaa ccttcgtgaa ggagacacag
     9121 ttgttggtgc cagcttgatt actgatcaag atgaggttct tattatcaca gaaaaaggat
     9181 atggtaagcg tacagtcgct actgaatacc caacaaaagg tcgtggtggt aagggaatgc
     9241 agacagctaa aattaccgaa aaaaatggct tgctggccgg tcttatgact gttcaagggg
     9301 atgaggattt gatgattatc actgatacag gtgtcatgat tcgaaccaat cttgccaata
     9361 tttcacaaac aggacgtgca actatgggag ttaaagtaat gcgcctggat caagatgctc
     9421 agatagtgac tttcacaacg gttgcggtgg cagaaaaaga agaagttggg acagaaaacg
     9481 aaacagaagg tgaagcataa tgtctcaaaa aaataataaa aagaaaaaca agcgaaaaaa
     9541 tctgctgaca aatatcctag caggatttct gatattactg tcactggctt tgatttttaa
     9601 tactcaaatt cgaaatattt tcatagtctg gaataccaat aagtatcaag ttagccaggt
     9661 atcaaaagaa aaattagaag aaaatcagga tacagaaggc aattttgact ttgattctgt
     9721 caaagctatc tcttcggaag ctgttctaac ttctcaatgg gatgctcaaa aattaccagt
     9781 tattggggga attgcaattc ctgaattgga aatgaatttg ccgattttta aaggacttga
     9841 taatgttaat ctcttctacg gagctggtac aatgaaacgc gagcaagtaa tgggagaagg
     9901 aaattatagt ctagctagtc accatatctt tggtgttgat aatgctaata aaatgttatt
     9961 ttctccttta gataatgcta aaaatggcat gaagatttat ctaaccgata aaaataaagt
    10021 ttatacttat gaaatacgtg aagtcaaacg tgtgacaccg gatcgtgttg atgaagttga
    10081 tgatagagat ggggtcaatg aaatcacatt agtaacctgt gaagaccttg ctgctacaga
    10141 acgtattatt gtcaaaggtg atttgaaaga aacaaaagat tattcacaaa catctgatga
    10201 aattctaaca gctttcaatc aaccatataa acaattttat taatacaaat cagt
//
LOCUS       DAVLVF010000065        10207 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVLVF010000065 DAVLVF010000000
VERSION     DAVLVF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.065"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(89..1303)
                     /gene="thiI"
                     /locus_tag="VYN66_001532"
     CDS             complement(89..1303)
                     /gene="thiI"
                     /locus_tag="VYN66_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV1495536.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            complement(1312..2454)
                     /locus_tag="VYN66_001533"
     CDS             complement(1312..2454)
                     /locus_tag="VYN66_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1495537.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            2592..3020
                     /locus_tag="VYN66_001534"
     CDS             2592..3020
                     /locus_tag="VYN66_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495538.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
     gene            complement(3171..5396)
                     /locus_tag="VYN66_001535"
     CDS             complement(3171..5396)
                     /locus_tag="VYN66_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1495539.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(5560..7512)
                     /locus_tag="VYN66_001536"
     CDS             complement(5560..7512)
                     /locus_tag="VYN66_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1495540.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHSYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQVP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            complement(7509..8420)
                     /gene="pfkB"
                     /locus_tag="VYN66_001537"
     CDS             complement(7509..8420)
                     /gene="pfkB"
                     /locus_tag="VYN66_001537"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV1495541.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            complement(8417..9157)
                     /locus_tag="VYN66_001538"
     CDS             complement(8417..9157)
                     /locus_tag="VYN66_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1495542.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            9320..9407
                     /locus_tag="VYN66_001539"
     tRNA            9320..9407
                     /locus_tag="VYN66_001539"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9354..9356,aa:Ser,seq:gga)
     gene            complement(9590..9937)
                     /gene="ssrA"
                     /locus_tag="VYN66_001540"
     tmRNA           complement(9590..9937)
                     /gene="ssrA"
                     /locus_tag="VYN66_001540"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         2987 a         2315 c         1955 g         2950 t
ORIGIN      
        1 aaaataaaaa aagttagttt tttacctgtt tttagagaaa aaactaactt tttttactga
       61 ttttcgctat tcttttggat tttctgaatt agagcagatt gtcaatcaag tcatcaactt
      121 catctttttc ggcttgaggt gtgatttcag taatcatgat tccagccact gctcgctcaa
      181 ccaagccttc aacatccata cgcgcttcgt actgctccgc attcttaatt ttaggatttg
      241 tttttggacg atctggtgca aaaatagtac aacagtcttc aaacggttgg attgaaatgt
      301 caaaggtatc gatttcctgg gcgatgtcaa tgatttccaa cttgtccatg gtaaccacag
      361 gacgaatgat gggagtgttg gtaacagcat tgatagcctt catactttca agggtttggc
      421 tggctacttg acctagactt tccccattga tgataactaa accatttcgt acctcacgaa
      481 tacggtcagt aatacgcatc ataaaacgac gcgtcaaggt cataaggtag gcttctggcg
      541 ctttggcttt gatttcctct tgaatctctg taaaaggaac ttcgataaac tggatatttc
      601 ccccaaattt ggttaattta cgggtcaaat cttgggcttt tttaagagca ccaggactcg
      661 tgtaaggtgg gctggcaaag tgaactgcct ctatatctac ccctcgttta agcgctagat
      721 agccagctac aggggaatcg attccccctg acaacatgag catgccctta ccagaagttc
      781 ccactggtaa tccacctgcc cctcgaacgg tttcataaga aagataggct gcttcttcac
      841 gtatctccac ctgaagattg atgtcaggac ttttcatttg agcttgtacg tttggaatgg
      901 cttcgaatac agcccctcca agcgtttggt tgagttcacg actgtccaat tcaaaggtgt
      961 ggtcgctacg cttgctagag attttaaagg tcatgccttc cttgtagatg tcctgcataa
     1021 tctcttggac agcagacttc agaacttcta cagatttttc aaccttataa acaggggaaa
     1081 agttttgaat tccaaatact tgtttgagcg attctgctac tgctgtgtaa tctgctccat
     1141 tgaggtaagc gtgggcacgg tcgcgatctg ctgttacctt aacttgggga tagatagaca
     1201 aaacgtctga aatattattg cgaagtttat tgatgaaacg catacgattt ttgcccttgg
     1261 ttgacagctc accgtagcga atcataattt ctgaatactg catgaatgct cctatcttac
     1321 ttttctagtt tgattgtaaa ttaattttag cttggtcaag aactgctcga cctgactcat
     1381 atcattttca aggtctagac taagacgcac agctgactga gccttatcct tgtccactcc
     1441 catagcaatc agggttccgg ctggtttccc agccttggac gaacaagccg aggtcgttga
     1501 gatgaaaata tcatagtctt caaaggcgtg aacaatgacc tcaccacgaa cacccttgat
     1561 tccaaaagtc agaatatgag gggcgaagtc ttcctcatct gaaaagacaa atatatccgg
     1621 atagtccaga agtgcttgac gaatcactgc cttcatctgc ccagtcttgc tcctaaagat
     1681 atctagcttt tccatagata aacggagagc cttagcggtc gctgcaattc ctgccacatt
     1741 ttcagttgtc gaacggtaat cacgttcctg accaccacct gttaaaagag gcgtaatctt
     1801 cttgccagac ttgatataga taaaaccaac accacggatt ccgtggaact tgtgaccaga
     1861 gaaagtcgcg aaatccactc gttctgtcag atacttttca gtcggaatct tggcaagcgc
     1921 ctgaaccgca tcaacgtgga aggaaatagt tggcttgtct gccaagaatt ctgaaatagc
     1981 ctcaataggt tggatagagc cgatttcatt gttcacagcc atgatggaaa cgagggtcgt
     2041 atcaggtctt atcaaatctt ctagcgcctc aacatccaca aatcctttgt tatcaactgg
     2101 agcaaaatcc acttcaaaac cttgagcttt caaccagagg gctgattcct taactgctgg
     2161 atgttcaatg gctgatacga tgatgtgctt gccaaactgg gctttttcaa atgctactcc
     2221 cttgatgatc cagttatccc cttctgtccc accagatgta aagaagattt catcactttt
     2281 cttaccgatt aaatctgcaa tctgttgacg ggaagcatct aaaattcgtg ttgcctggtc
     2341 tcccaaacga tggagactag atggatttcc taaaattttt gaagccacct gcatataggt
     2401 ttcaagggct tctggatagg gcctggtcgt cgccgaatta tcaaagtaaa tcatgtttct
     2461 cacgctttct aaaatcactc cttctattgt atcatgaaac ggagcctgcg acaagaaagg
     2521 gcaatttctc tttttttaag atttttttaa agaaacagag tataataaat cataatcaaa
     2581 ggagagaatg tatgtctaac tatcgtagaa cttcaaaacc gaaaacagaa catatcaaaa
     2641 aaggctttac tgtttttcaa aaaaccatta ctactatcgg tagtatcctt ggtttgatta
     2701 ccgcgggtat cacgatcatg aacgccttgg ataataataa taaaaatact aaaaaagaac
     2761 ctacgacaag ccagacgaca acctttgtca aagaaattca aaaagaatcc cctcaggaaa
     2821 atactactcc aaataaggaa aataacactt ctcaagaaaa aacacaacaa gaagaaacgc
     2881 caaaatctag cgtcaaggaa gagaaaaaag aagatcagaa aacagcaact caggactcta
     2941 ctacacctgc tacaagtaaa cctgccactg aaaatgaaaa acagcccaat actccaactt
     3001 cagaaaataa tactcaatga aaatcaaaga gcaaactagg aagctagccg tagacagtac
     3061 ttgagtacgg caaggcgaag ctgacgtggt ttgaagagat ttgcgaagag tataaaagta
     3121 atcaatagcc agtaaaatag ctccttccaa ccttggaaag aagctatttt ttattgctgc
     3181 aatacttttc ttggcttggt accttcagct ggaccaatga cacctgccat ctcaagctct
     3241 tccatgagac gggtcgcacg gttaaatcca actgacaaac gacgctgaat catggatgca
     3301 ctggctttct gtgtttcgat aaccaaagac ttagcttctt caaaaagcgg atcaccacca
     3361 gcatctccat ccgaaaattc tccttcattt tcagaaacct cacctggatc aaaactctca
     3421 tcgtagtctg catctgcctg agtcttgatg aagttcacaa tgcgctcaac atcgtcatcc
     3481 gagataaagg agccttggag acgaactgga tgattttcat caatcggttt aaagagcatg
     3541 tctcctcgac caagaagttt ttctgctcca ttttcatcca aaatcgtacg ggagtctgtt
     3601 cctgatgaaa ccgcaaatgc tacacgagat ggaacattgg ccttaatcaa accagagatg
     3661 acatcaacag atggacgctg agttgcaaga atcatgtgga tacctgcagc acgcgccttc
     3721 tgcccaagac ggatgatagc atcttccact tccttgctgg ccaccatcat gaggtcagcc
     3781 aactcatcca caatcacgac aatgaatggt agcggaattt gcttgtactc agactgggaa
     3841 ttgaactctt ctaccttggc attaaaacct gcaatattcc gaactcccac cttggcaaag
     3901 agttcataac ggttttccat ttcatccaca accttttgca gagccttgct ggctttgcgt
     3961 ggattggtca cgactggaat caagaggtgg ggaatatcat tgtaaacaga taactcaacc
     4021 atcttgggat cgaccatcat aaatttaact tgatctggtc tcgccttcat gagaatgcta
     4081 gcaataatgc cgttaactgc tactgacttc cctgaacccg ttgaacctgc aactagcaag
     4141 tggggcattt tagaaaggtc aaaagctctt gcggttccat taacagcctt ccctaaagga
     4201 atttccaaga aattttctgc tttcgtttgc gattgttccc atagttctcg gaaagataca
     4261 gtggcaatat cggagttggg cacttcaatt ccgattaggg atttcccagg gattggtgct
     4321 tcaatccgga catctttggc agccaaggct agagcgaggt catctgatag attggaaatg
     4381 cggttgaccc ttacaccaac agccggcttg acttcatact tggtcactga tggcccaatt
     4441 tcggcccgtt caactgttac cttaatacca aagctagcaa aggttgcttc taagattttg
     4501 atattttctc tgacaatttt cttctcttta gactgatctt ttggtttatc tggtgcaaag
     4561 agttgtaagc ttggaagttt gtattcaagg gcttctttgg ctgaaaaatc gacctgaaca
     4621 tcttcgtcat ctgagtcatc ttcctgttca gggaatttaa gttcagcttg aggcaggatg
     4681 atttctggtt ccacccactt ttcttctgga ataggtggaa gattttgaac agcttcctct
     4741 gtcagaattt cacccgtttc catatcaaca ggaggcaaat cgagtaaggc tttttcagtc
     4801 tcttcttgtt ctaatctagc ctctttctca gccttttggc gagctttttc ttcttgtttg
     4861 acaaagcgtt cctcttttcg acgctcgtgc ccttcccacc atttggcaaa gcctctactg
     4921 aaaaattcag caatatcgta aacagaccaa gggctgacta ggagagaacc cactaaaatc
     4981 aagatagaac caataaagta agttccgata tttgaaaaga gaaaagctgt tggaatataa
     5041 agagcgaccc cgatcaagcc ccctccagca aaactagtcg ttcgaaaacc agtcagatct
     5101 gtcacaacct gagccatggt cccttttaga acggacttgt ccaaaccata tttccaaacc
     5161 aagtaggctt caaaaatcaa gagtaagcca gcaaatatgg tgaaaaagcc agataagagt
     5221 ccttcctgtt ttcgtatcca cttgaaaaaa aagagataga ttaataggcc gaatatcgcc
     5281 agataagcta ggctacccac tagcaagcga attaaattat aaagggttat acctgcagcc
     5341 cctaatttga aggctgcgaa aatcaataaa atcgcaattc ctaacgaaat caacattcgt
     5401 tgaatcgctt cttttctttc cagttctgct ttagacggtc tccgtcttgt tgtacttgta
     5461 ttcttgtttg ccattcttct attataccat atttcacagg catttcggaa agagaaaaag
     5521 actgcaccaa acggtccaat cttttcattt ttctattttt tatgcttgtg gtttgcgtag
     5581 gtaaccataa accacaccac ttacgattgc tcctaccaag acagaaacga ggtaaaggag
     5641 agcatttgaa gtaagggcga taacgaagat tcctccgtgt ggcgccatga gtttaatacc
     5701 agtaagacca acgagtccac ctgctactgc tgaaccaagg atgaagcttg gaatcgcacg
     5761 agctgggtca gcggcaccaa atggaatcgc tccctcagtg ataaatgaca agcccatgat
     5821 gatgtttgtc aaaccagagt tacgttcttc cttagtaaat ttatctttga aaagaagagt
     5881 tgcgacaaag attgcaagtg gtggcaccat tcctccagcc ataactgctg ccatggctac
     5941 agaaccacct gaagaaacag ttgctgcaag cgtacctgta ccaaagacat aagctgcttt
     6001 attaactggt ccacccatgt caacagccat cattccacca aggacgatac caagaaggac
     6061 agctgaacct cctccaagac cgcctaggaa gtcattcata gcagtgttga ttgctgccat
     6121 tgggatattc acagctagca taacaaatcc tgtcaagatt gttccaagaa gtggcaatag
     6181 aaggattgat ttagcacctt cgagtgaacg aggaacttta acgtatttct tgatggcaag
     6241 aaccaaggca cctgcgataa atccaccaac aagggcacct aggaaaccag atgagacacc
     6301 tgcaagagtt gaagttgctt caccacctgc ggcataagga attttaccaa aggcaaaacc
     6361 ttctttggca atagcaccag ccacgaaacc tgctaccaaa cctggttttt cagcaataga
     6421 gtaggcaaca taacccgcaa agactggaag catcaaacca aaggcagctc caccaatttt
     6481 catgaacata gaagctaact catggtaaga accaagattg ccaaggtttt catttggaac
     6541 acccaaagca ccgtcaatca agaaggcaag ggcaatcatg ataccaccac cgataacgaa
     6601 tggtaacatt tgagatacac cactcatcaa gtgtttgtag aaggcaccac caaggctttg
     6661 tttttcgtta gaggctgttg cagcttttgc accattagcg gcacggtaga cttcagtatc
     6721 tcctgaaaga gccaagttaa ttagctcttc tgtcttacgg ataccgtcag caactggacg
     6781 attgatcaat ggttttccat caaatcgatc catttcaacg gccttgtctg ctgcaatgat
     6841 aatagcttta gccttacgga tatcttctgc agttagttga tttccaacac cgctagcacc
     6901 gttggtttcg accttgatac caacccccat ttcagcagct actttttgaa gggcttcttg
     6961 ggccatgtaa gtgtgggcaa tacctgttgt acaagctgta acagctacga taaagtcacc
     7021 agagtcatta gcaggtactt gaacaagttc ctcagttttt tctgaagctt ggtcaaaaag
     7081 ttcgataact tggtctgcag atgttgcttg acgaagtttg tctgcaaaac cgtctttcat
     7141 caagtattga gacaattctg ccaaggctgc caagtgagta tcattggcac cttctggagc
     7201 tgcaatcatg aagaagaggt cagttgcttg tccatccaag ctctcgtagt caacaccctt
     7261 atttgactta gcaaatagaa ctgtcgcttc tttgacagca gcgtttttgc tgtgaggcat
     7321 tgcgattcca tcacccaaac cagtagaagt caaagcttca cgcgccaaaa ttccttcttt
     7381 aaatgtttca aaatctgtta cataactgtg ctctgtcaaa tttttaatca tctcgtcgat
     7441 gacagctgtt ttttcagttg cctgcaaatc tagcaacatg acatcttttc tcaataggtc
     7501 ttgaattttc atcttttttc tacctcaact tttccatatg tttctttaat aaattccgcc
     7561 gttgccaagt catctgagaa ggtagttgcc gttccgcaag ccactcccca tttgaaggct
     7621 tctactgcgt cttttgattt gacaaattca cctgtgaatc cagcaaccat agaatcacca
     7681 gctccaactg aatttttgac tgttcctttg attggtttag cgaagtaagc tccctcagat
     7741 gtgacaagaa gggcgccatc accagccata gagataataa cattttgagc acccttagcc
     7801 agtaactcac gagcgtattt ctcaatttca tctaaacttt cgagtttaac cccaaaaatc
     7861 gctccaagtt catgattgtt tggttttaca agaagaggct ggtagtccaa actatcaatt
     7921 aaggtctgtc cttcaaagtc acagaccact tgcgcaccag tctggcgcgt caaggaaatc
     7981 aaatccttat agataacatt gcctagattt ttagcacttg aacctgcaaa gacaactgta
     8041 tcttctgctg tcagactaga taaaatagct ttcaattctt ctagctgaac cggttcaaca
     8101 gttggacccg ttccgttgat ttctgtttct tggtctgctt tgattttaac attgatacga
     8161 gtatcttctg ccacctggac aaaacgtgtc tcgatttctt cctctgctaa agtatctgtg
     8221 ataaatttac cagtaaagcc accgataaat cccgtcgctg tatttggtat attcaaacgt
     8281 ttcaagacac ggctgacatt gattcctttc ccaccagcaa acttatcatc actgtccata
     8341 cgatttacac taccaacttt gacttggtcc aaacgaacga tatagtcaat ggatggattg
     8401 agtgtgactg tataaatcat acttctatta cctccgtttt ctccttaata acctgcaaga
     8461 gctcatgccc ttgactagtg ataacgatag cgcgtttgag tggtgctacc ttggcaaagc
     8521 aagtttgtcc aatttttgac gaatccacca agacgtaggt ctgcttggca ttctccaaaa
     8581 tagctctttt cacagctcct tcctccatat caggagtcgt ataatagcca tcgtcaacac
     8641 cattcattcc gataaaggca cggtcaaagt gcaattggtt aatctggtta agagcaacgc
     8701 ccccgatact agcatctgtc gccgtcttga cgtttcctcc aaccatgaca gttggaatct
     8761 gcttttcaac caactgagcg gcatggtgaa tggagttggt cacaactgta acattcttat
     8821 tgaccaattc atgaatcaaa aaagcagttg ttgttccagc atcgataaag atgacatctt
     8881 tttctttaat gagagaggct gctttctgag ccagcaactt cttttcttga aggtttttga
     8941 cagatttttc ttgaatggtt tcttcttcct gtaaggagtg ggggagttct gctccaccat
     9001 gcacacgacg aagcttgttt tccgcttcca actcatccaa gtctcttcga accgttgatt
     9061 ctgacgtttc tagcaaacta actaattttt ctagagaaac tacatgatgt tgatttaact
     9121 cctctaaaat tagttgttta cgttctgttt ttaacactga atcacctcct gttatcgttt
     9181 acactattca ttctatcata ctttctttcg aagtcaagca ttttttatca ttttctttca
     9241 aaatctatca ttttttctta tttctagaat ttttattgca agataagagc ctttatggta
     9301 gactatttga gtaagtattg gaagattact caagaggctt aagaggccgt gttggaaacg
     9361 cggtaggcgt gtaatagcgt gcgtgggttc gaatcccatg tcttccgtcg aaaaaagatt
     9421 atttctagga tgcgacaaat gttgtatcct tttttctatc ctttttttaa aatagggtct
     9481 gtttgtgact ccctttttag caaaagaatg caaaggaata ccaaagataa aaataaaaaa
     9541 cgttgattta acaacgtttt accaaggaat gcaaaagaat gcaaaggaat aatggagccg
     9601 gtgggagtcg aacccacgtc caaacacctg ccaacatatt tgtctacaac cataggttat
     9661 gtattatttt aacaacccct cgacacataa ctcaagtcta gaaactgcga gtctatcaat
     9721 ctcttatcaa gccgctagac aaggcttaat cgtatctcgc taataataag acctgtcatt
     9781 gaacacgagc aatccaaatc gggtcacgcc tgctggtttt taggcagcta gagcgtaaga
     9841 agtgttattt tttgcagtta tatttaactg agcgtttacg tcgccacacg agtcgcaaaa
     9901 tatgcctcat aatgcctgtc gaatccgtaa cgaccccaaa agacaataaa accattatac
     9961 cttatctaac tcaaaaatgc aaaaagaaat cacataccta aaaaagatac tattcgagaa
    10021 atttagcaaa ctggaagact aactacaact ttctcagtgc ataactttac gattgtaaat
    10081 agagcttacg aaatccaatt aacacatacc taagatgaag ctatctgttt tgaagaaatt
    10141 cacgaagaga attattctaa aatactttca gatgagaatt ttcgtactct ctttatttta
    10201 attcatt
//
LOCUS       DAVLVF010000066        10101 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVLVF010000066 DAVLVF010000000
VERSION     DAVLVF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.066"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..211
                     /locus_tag="VYN66_001541"
                     /pseudo
     CDS             1..211
                     /locus_tag="VYN66_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558102.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            309..521
                     /locus_tag="VYN66_001542"
                     /pseudo
     CDS             309..521
                     /locus_tag="VYN66_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            421..867
                     /locus_tag="VYN66_001543"
     CDS             421..867
                     /locus_tag="VYN66_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1495543.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1005..2309)
                     /gene="eno"
                     /locus_tag="VYN66_001544"
     CDS             complement(1005..2309)
                     /gene="eno"
                     /locus_tag="VYN66_001544"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV1495544.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2473..2919
                     /locus_tag="VYN66_001545"
     CDS             2473..2919
                     /locus_tag="VYN66_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV1495545.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAKPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2916..3719)
                     /locus_tag="VYN66_001546"
     CDS             complement(2916..3719)
                     /locus_tag="VYN66_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV1495546.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3728..4057)
                     /locus_tag="VYN66_001547"
     CDS             complement(3728..4057)
                     /locus_tag="VYN66_001547"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEV1495547.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(4002..4373)
                     /locus_tag="VYN66_001548"
     CDS             complement(4002..4373)
                     /locus_tag="VYN66_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV1495548.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4432..5865)
                     /gene="glgA"
                     /locus_tag="VYN66_001549"
     CDS             complement(4432..5865)
                     /gene="glgA"
                     /locus_tag="VYN66_001549"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV1495549.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYCWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5862..7001)
                     /gene="glgD"
                     /locus_tag="VYN66_001550"
     CDS             complement(5862..7001)
                     /gene="glgD"
                     /locus_tag="VYN66_001550"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV1495550.1"
                     /translation="MKIDKYSAILGNTVGFHDMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVELGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(6991..8133)
                     /locus_tag="VYN66_001551"
     CDS             complement(6991..8133)
                     /locus_tag="VYN66_001551"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV1495551.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8123..10051)
                     /gene="glgB"
                     /locus_tag="VYN66_001552"
     CDS             complement(8123..10051)
                     /gene="glgB"
                     /locus_tag="VYN66_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV1495552.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELREKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3069 a         2234 c         1790 g         3008 t
ORIGIN      
        1 gaaccacacc tactatgaac aagacccaga aaaagtagcc ttatttctta agaattttaa
       61 tagtttaaag cacctagcac aggtctaaca aatgaatggt gagttaatcg ctccaatgac
      121 ttacgaagag acgatgacga gcgacttttt tgaagcttgg tttcagaaat ttctcttacc
      181 aacattaacc gcaccattgg ttattatatt aaaacgaagt aagaatagga caaatcgatc
      241 aggacagtaa aatctaattc taacaatgtt ttagaagtag aggtgtacta ttctagtttc
      301 aatctactat attaatggac aatgcaagat tccatagaat ggggaagcta gaactcttgt
      361 gtgaagagtt tgggcataaa cttttacctc ttcctcccta ctcgcctgag tacaatctta
      421 ttgagaaaac atgggctcat atcaaaaagc acctcaaaag ggtattacca agttgcaata
      481 ccttttatga ggctcttttg tcttgttctt gtttcaattg actatataaa ttgtctaagc
      541 gaaacaaccg ataagaattg gcacaaaagc gaccgtattt ttgttaccaa tacaggaaaa
      601 ccagttcata gttctatctt gagcaagtct ctccagcgag caaacgaacg ccttaaaaaa
      661 ccaattccca aacatctgtc ccctcacatc ttcagacaca ccactattag catcttatca
      721 gaaaataaaa ttcctttaaa aacaatcacg gacagggttg gtcattccga ctctgaagtc
      781 actacttcca tctacaccca cgtcacaaag aacatgaaag atgaagcaat caatgtactg
      841 gataaagtta tgaaaaagat tttttaaaaa gttttgtccc ttttttgtcc tctaaataca
      901 aaaatagccc ttcggataaa atccgagggg ctagaaacgt tgttaaatca acggccgaac
      961 ttttgaattt catggttcgg gataaaatag ttcactgaac tattttattt tttaaggttg
     1021 tagaatgatt tcaatccacg atattcagct acttcaccaa gttggtcttc gatacgaagc
     1081 aattggttgt atttagcgat gcggtctgta cgtgaaagtg aaccagtctt gatttgtcct
     1141 gcgttagttg caactgcaat atcagcgatt gttgaatctt cagtttcacc tgaacggtgt
     1201 gatacaacag cagtgtaacc agcttcttta gccatttcga tagcttcaaa agtttcagta
     1261 agagtaccga tttggttaac tttgataagg attgagttag cagcaccttc ttggatacca
     1321 cgtgcaaggt agtcagtgtt tgttacgaag aagtcgtcac caacaagttg tactttctta
     1381 ccaagacgtt cagtaagagc tttccaacca tcccagtcgt tttcatccat accatcttca
     1441 atagtgatga ttgggtattt gttaaccaat tcttcaaggt agtcgatttg ttctgcagat
     1501 gtacgaacag cagcaccttc accttcaaat ttagtgtagt cgtaaacttt acgttcttta
     1561 tcgtagaatt ctgatgaagc acagtcaaat ccgataaata cgtctttacc tggtacatat
     1621 ccagcagctt caatcgcagc aaggatagtt tcaacaccat cttcagttcc ttcgaaacga
     1681 ggagcgaatc caccttcgtc acctacggca gtttccaaac cacgtgattt aaggattttc
     1741 ttaagagcgt ggaagatttc agcaccgtaa cgaagggctt ctttaaatgt tggcgcacca
     1801 actggcaaga tcatgaactc ttggaaagcg attggagcgt cagagtgaga accaccgttg
     1861 atgatgttca tcattggagt tggaagaact ttagtgttga atccaccaag gtagctgtaa
     1921 agtgggattt caaggtagtc agcagcagca cgagctacag cgatagacac accgaggatt
     1981 gcattcgcac ccaatttacc tttgttagga gtaccgtcaa gtgcaatcat agcacggtca
     2041 atagcttgtt gatcacgtac atcgtagcca atgatagctt cagcaatgat gttgtttacg
     2101 ttgtcaacag ctttttgtgt accaagacca ccgtaacgag atttgtcacc gtcgcgaagt
     2161 tcaactgctt cgtgttcacc agtagaagct cctgatggaa ccataccacg tccgaaagca
     2221 cctgattcag tgtaaacttc tacttcaagt gttgggttac cgcgtgagtc taggacttcg
     2281 cgagcgtaaa catcagtaat aattgacatt ttttactctc cttatgagtt aaatttttta
     2341 cacctctata ataccttaaa acccctcctt tttcaagaaa aaacgttatc tttgtgcaac
     2401 ttttccttaa ctttataaag taatcgcttt cttttgtctg ttttattcta acttttatga
     2461 tatactgttt tcatgacaga tttatcaaaa caattacttg aaaaagctca tggtgggtta
     2521 aaaataaatc cggatgagca aagacgctat cttggtactt ttgaggaaag agttcttgga
     2581 tatgtagata ttgacacagc aaatagccct cagttagaaa aaggcttttt atttatttta
     2641 gaaaaccttc aggaaaaagc aaagccacta tttgtgaaga tttcaccaac tatcgaattt
     2701 gataagcaag ttttctactt aaaagaagca aaagaaactg atagtcaagc caccatagta
     2761 tctgaagagc atattacttc tccttttggc ctggttattc atagcaatgc accagttcaa
     2821 gtagaagaaa aagaccttcg acttgctttt ccaaaacttt gggaagttaa aaaggaagaa
     2881 ccagccaaaa catccttatg gaagaaatgg tttagctaaa tcttgcacat atttaataag
     2941 tgcccaatat tggcagccgt gcgctccaga tagagactgg catttttcaa actatcttct
     3001 aaaggttcac ttttctccaa aatagaaaag acagcttgga tattttcaaa tggtagggga
     3061 ggtaaatctt cagcaagact accgcaaata gcaataacag gaactccaac aggggttctt
     3121 tttgcaacac ctataggcgc tttcccagca aagctttgac tatcaagtct tccttctcca
     3181 acaacaacca agtcagcatc tgaaactttc ttatcaaagt tgattaagtc caagcaggta
     3241 tcaattccag acacgatact tgcctgagca aaggcacaca aaccagcagc aatccccagt
     3301 cctccatcat tactggccgt gccaccaaca ccgatataaa tatctttaat ccctttagaa
     3361 atgagatgga gaatcaactc tccaatacca caagtttgga tttgaagtgg atttcgtttc
     3421 tctagcggaa tttttccaag accaaccaag tcagctactt caaatagtgc cagttcccct
     3481 ttttgaaaat agcgcatggc ttctttttgt ccaaaagggt ctgtcacttg gatccatttt
     3541 tcttttaggt caagagaatg tcggatagca tctacagtac cttctccccc atcaccaaca
     3601 gggcagagga gacattctac atctgctatc gattgttgga agcctctttt tattgcttca
     3661 gctacctgtt gagctgtcaa gctttcctta aacgaatccg gtgcaattac aatcttcata
     3721 ttttccctca ttctaaacag tcaatcaaag gaagaacttc taaaaaatcc ctcttgtcaa
     3781 catgatgtgg tatttctttt ttgagcattt ctttggaaca aaaggcgatt cctaactccg
     3841 ccgacttcaa cattaataga ttattaaccc catcaccgat tgccaccgtt ctttctttag
     3901 aaagttttag tttctttctc catttttcca gagtttcttt tttgacctgg ggacttataa
     3961 tttgtccaac taattttcct gttaaaagac cttctttgac ttcaagctgg ttggcagtga
     4021 aataggcaat accaagggat tttgctaatc tctccaacta ttggtgtaaa tccaccagac
     4081 accagaccaa ctaggatgcc attcttttgg agaatagaga tgaactctgg gacatttagc
     4141 gatagatgaa ttgagttgaa gacgttatca aagaccaaaa taggaagacc ttccaacaag
     4201 gacactcttt ttcttaaact gctttcaaag accaactctc ctcgcattgc tcgacttgta
     4261 atctgcgaaa tttccgcctc atgacctgcc tctctcccta aaagatcaat cacttcttct
     4321 aggattaagg ttccatctac atccaaaaca cacaagcctt ttacttgaga catcagttct
     4381 cctctctaaa cagcctaaaa atcgtatgaa gtcatcatac gattttatct attaattaac
     4441 taaactatgg tacaagtcaa ggtatgactt gcaggctgta tcccatgaga agtcactctc
     4501 catagcttgt ttttgtaggt ttctccaaat gtctggatgg tttctataca agtccaatgc
     4561 tgtttggaaa gtccaattta accaataagg agatagattg tcaaagctaa agccagtacc
     4621 gcttccttcg attggattga aagcgcgaac tgtatctcgc aagcctccaa cttcatggac
     4681 caatggcaag gttccataac gcatagccat catttgagac aagccacacg gttcaaaacg
     4741 acttggcatg aggaagaggt cacaagcagc gtagatttcc tgagcaagtt tgacatcaaa
     4801 agtgatattt gctgatagct tgtctgggta aatctgagca aaccatgaga aagctccttc
     4861 aaaggctgga tcgccagttc ccaaaagaac aatctgaaca tcttcttgca agatatggtg
     4921 aagactttcg accaccacat caaaaccttt ttgacgtgtc aaacgagaaa caattcccac
     4981 cagtggaacg tctgctctaa caggcaagcc aactctttct tgcaattttg ccttattttt
     5041 ggctttccca gacaaatctt cctgattgaa atgatagtct aaaagagcat ccgtctgagg
     5101 attataaaga tcagcatcaa tcccattcac gataccagat actttaccag actccatttt
     5161 aagaatctga tccaaattac atccaaactg actagtcata atttcatgag catagctagg
     5221 tgaaacggtt gaaacacggt tcgcatagag aatacctgcc ttcatccagt tcagacagtt
     5281 gttccatcga agggtgccat cagcgtaacg ttcaaggcca actccaaaca aatcacccaa
     5341 cattccttct gaaaattgtc cttggaattc taaattatga atggttaaaa ctgtttcaat
     5401 gtcctcatag gcttgaatcc aacagtattt ttccttcaac aagaaaggaa tcatagctgt
     5461 atggtagtca tgaacatgga gaagatcagg aataaagtca atcctttcca tagcctcaat
     5521 ggcagccagt tggaaaaagg caaagcgttc tccgtcatca aaatcaccgt aaacatgacc
     5581 acggaagaaa taatattgat tgtcaataaa gtagaaggtt acaccattta atactgtttt
     5641 cttaattcca caatactgtc tacgccaacc aacgctcacc tcaaaatgaa gcacatcttc
     5701 aatctgattt ccaaatttag cctctaccat atcatagtag ggtaaaatca ctgcaacttc
     5761 gtgcccagct tttaccagtg attttggaag agcgccaatg acgtctccca aaccacctgt
     5821 ttttgaaaag ggtgcaccct ctgctgctac aaataaaatt ttcatgaatg aatatcctct
     5881 gttactttag cacctttctt aaccacaact ggatgttctg cagttcctcg aatcacaaca
     5941 ccaagctcaa cttcaacccc tttgtccaag atagcatatt cgacctgagc cccttctcca
     6001 ataacaacac gagggaagag caggctatct ttaaccaagc tatccttatg gacatgaata
     6061 ttacgtgata gaacagaatt agctacttga ccttcaataa tactaccaga ggcaaactga
     6121 gaagtgctta ccttagatgt attagcatag taagttggct cttcgttttt gacctttgta
     6181 taaatctttt ggtttggtga gaaaagagaa tagaattttt gtgattcaag catatcgata
     6241 ttcgcttgat aataagattt aacagagtga atattggcta gatagcccgt gtactcgtag
     6301 gcaaaagctc cctcttttac agccaaatcc cgtaaaacat agcgcaattt ctctggatgt
     6361 tcttttttag cttcttcttc caagtgttca atcaaccaag gtgtatcaac gacaaagata
     6421 tctgtagaca tattgaacgt ttcagctgtt gacttgctat caaagagttt atgagaaaga
     6481 acatggtctg tttcatctac atccaagatt gcatttactt ctgaaatatc tttcttagct
     6541 agttttttat aaactacagt gataggctct tttgttgtac tatgtaggtg gaaaacttgg
     6601 ttcaaatcaa tgttaataag aacatcgcag ttgagggcaa ccgtttggtt tgagccagaa
     6661 cgtttcaaat aagtaagaag ctgttggtag tattcttttc caactgtact actttctaca
     6721 cgggtattgt aaattcctag atagtaatgg ctaagaaggg ttgataagcc ccactcgcgc
     6781 cctgaacgaa tatggtcaaa gactgagctg atattatcct gctggaaaat accaaagaca
     6841 ctacgaacac ctgcattagc aaggcttgaa agtgggaagt caatcaaacg atatttccca
     6901 ccaaatggca aacttgctac tggacggtgg tccgtcaatg tcgacatatc gtgaaaacca
     6961 actgtatttc ctaaaatggc agaatattta tcaatcttca tctgttgcta cccccactac
     7021 ttcattatat cctacaactt gtacttcatc tgttccatca atttcgacac cgtcagaaat
     7081 aatcgcacct tcaccaataa tggcacgttt aatcttagct ccttgaccaa tgatagctcc
     7141 actcatgata actgaatcaa ggacttccgc tccttcgcga acttgcgcgc ctgttgaaag
     7201 gatagaatgt ttaacagttc catcaacgaa acatccgtct acaactaatg agtcttccac
     7261 atgagcattt gccccgagga agtttggtgg tgaaatcaag tttcttgagt aaatcttcca
     7321 ttgacggtta cgactatcca aggcattttc tggagaaata tactccatgt tcgcttccca
     7381 aagtgactca atagtaccaa catctttcca ataaccacta aattcataag cataaacact
     7441 ttcacctgac tcaaggtaat ttggaatgac atttttacca aagtctgaca tgccaacctt
     7501 gctcttttca gcagcgacta acatattacg aaggcgttgc caatcaaaaa tgtagattcc
     7561 catagaagct tttgtagatt taggttgagc tggtttttct tcaaattcaa caatacgatt
     7621 gttagcatct gtgttcatga taccaaaacg gcttgcttct ttaagaggga cgtctaaaac
     7681 tgctactgtc aagctggcat tattatcctt atgagactgg agcatatcat catagtccat
     7741 tttgtagatg tgatccccag acaaaatcaa gacatactca ggattgacac tgtcgatata
     7801 gtcgatattt tggtaaatag cgtgactagt cccctcaaac caacgatttc cttcacttgc
     7861 agaataaggt tgaagaatag agacacctga attaataccg tctagtcccc agcttgaacc
     7921 attcccaata tggttgttga gagcaagtgg ttgatactgt gtaacgaccc caacattatg
     7981 aatccctgag ttggcacagt ttgatagggc aaagtcaatg atacggtagc gcccaccaaa
     8041 ttgcacagct ggttttgcga tgctttgagt gagtttaccg agacgagttc cttgcccacc
     8101 agcaagaatc aaagctaaca tttcattttt cattttctac tcctttttgg tttttatttg
     8161 tgacggtttt agtagatttc aagcgacgtt tgattttcca tacacttgct cccatagccg
     8221 gtagggtaaa ggttaaggtc tgctcataat ctttccatag tccttcttgc gtttgaacag
     8281 tttgattatg ttctttccaa acgcctcccc actcttccaa ctcagtattc catacttctt
     8341 cgtaaattcc tgcaacgggt agtccgattg taaaatcttt ccgctcaaca ggtaccatgt
     8401 taaagataca gactaacatt tctccctttt tacccttacg aataaaggaa agaacactct
     8461 ggtctcgatt atccgcatca atgatttcaa taccatcata gctggtatcg atttcccaca
     8521 gacagcgatg atctttgtaa aactggttta gctgagaagt gaaatacttc atcttagcat
     8581 tcattgggtc ttctaggtta gaccattcca actgttcttc agatttccat tctaggaatt
     8641 gaccgtattc gctacccatg aagagcaatt tcttaccagg atgacaaatt tggtacgtat
     8701 agagattgcg caagcctgcg aattgattgt aacgatctcc ccacatctta tgcatcatac
     8761 tcttcttgcc atgaaccact tcatcgtgcg agaatggcaa gagataattc tccttgaaaa
     8821 catacataaa gctgaaagtc accaggttaa agtcatattt acgatagatc ggatcttctt
     8881 cgtagaaacg gaggatatca ttcatccagc ccatgttcca tttgtagtca aatcctagac
     8941 caccaatctc tttcattccc gtaatcttgg tcgcagacga actttcttct gcaatcatca
     9001 tcacatctgg atattctaac ttaataacct cattcaagcg ctgaaggaaa taataacctt
     9061 catagttgag atttccgcca tctttattag gtgtccatgg agcatcatca tagtccaaat
     9121 agagcatgtt gctaacagca tccacacgaa taccatccaa atgatagaca tcaatccaat
     9181 gcttaatgca agaaattaag aaggactgga cttcattttt tccaaggtca aaattaaggg
     9241 caccccaacc atggttatga gccttattat ggtcttggta ttcaaaagtc ggtgtcccat
     9301 cataataggc taaggcatca tcgttgatgg taaagtgacc tggtacccag tccacaataa
     9361 ccccaatatt atgggtatga cactcctcga caaaatcttg aaactcctct ggtcggccat
     9421 aagcatgctc taaagcgaag taacccataa gctgataccc ccaactcaag cccaaaggat
     9481 gggacatcaa gggcataaac tcaatatgag tatagttcat ttcaacgaga taaggaatga
     9541 gttcatcctt gagctgggca aaactataag gactgccatc agaatttctt ttccatgatc
     9601 cagcgtgaac ttcataaata ttgacaggac gctcttcaaa gccccaacgt tttcttcgtg
     9661 ccagccaaag tccatccttc catttcttct cacgaagctc tgttaagatt gcccctgttc
     9721 ctggacgagc ctcatacctg acagcaaaag ggtcaatctt catcagttga tgaccatttt
     9781 gacgtgtgac atgatatttg taaatatgcc cttcttgagc catattggta aagacttccc
     9841 agaccccaaa atcatttctt accattggaa tctgattttc aatccagttg gtaaaatcac
     9901 caaccaagtg aacagcctga gcattaggtg cccaaacacg gaaggtatag ccatgctctc
     9961 catttagttc ttccctatgt gctcctagat aatgttggag ataaaaattt tcacccgtca
    10021 taaaggtttt taatgcttct ctattatcca tatactcccc ttctcctgta agcgttttct
    10081 atgtttttat tatactacct t
//
LOCUS       DAVLVF010000067        10005 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVLVF010000067 DAVLVF010000000
VERSION     DAVLVF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.067"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(113..1135)
                     /gene="cbpF"
                     /locus_tag="VYN66_001553"
     CDS             complement(113..1135)
                     /gene="cbpF"
                     /locus_tag="VYN66_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HEV1495553.1"
                     /translation="MKLLKKMMQVALATFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRQEIAFRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSPAEKRVYYFEDQRSYHTLKTGWV
                     YDDGDWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLDPATGI
                     MQTGWQHLGNKWYYLRSSGAMATGWYQDGSTWYYLDAENGDMKTGWQNLGNKWYYLRS
                     SGAMATGWYQEGSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(1154..1975)
                     /gene="cbpG"
                     /locus_tag="VYN66_001554"
     CDS             complement(1154..1975)
                     /gene="cbpG"
                     /locus_tag="VYN66_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEV1495554.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHSYYRHDYGKEADDIYVLPAVSP
                     SQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKVHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYHLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(2056..2295)
                     /locus_tag="VYN66_001555"
     CDS             complement(2056..2295)
                     /locus_tag="VYN66_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495555.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(2445..2711)
                     /locus_tag="VYN66_001556"
                     /pseudo
     CDS             complement(2445..2711)
                     /locus_tag="VYN66_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2736..3047)
                     /locus_tag="VYN66_001557"
     CDS             complement(2736..3047)
                     /locus_tag="VYN66_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002877158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1495556.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYKHPELEEIILIFFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(2972..3203)
                     /locus_tag="VYN66_001558"
                     /pseudo
     CDS             complement(2972..3203)
                     /locus_tag="VYN66_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3204..3836)
                     /locus_tag="VYN66_001559"
     CDS             complement(3204..3836)
                     /locus_tag="VYN66_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1495557.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(3850..4845)
                     /locus_tag="VYN66_001560"
     CDS             complement(3850..4845)
                     /locus_tag="VYN66_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1495558.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENQSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(4842..5540)
                     /locus_tag="VYN66_001561"
     CDS             complement(4842..5540)
                     /locus_tag="VYN66_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV1495559.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(5617..6627)
                     /locus_tag="VYN66_001562"
     CDS             complement(5617..6627)
                     /locus_tag="VYN66_001562"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV1495560.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(6611..7618)
                     /locus_tag="VYN66_001563"
     CDS             complement(6611..7618)
                     /locus_tag="VYN66_001563"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV1495561.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDILIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVVSFEEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(7605..8558)
                     /gene="mvaD"
                     /locus_tag="VYN66_001564"
     CDS             complement(7605..8558)
                     /gene="mvaD"
                     /locus_tag="VYN66_001564"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV1495562.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEYLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(8540..9418)
                     /gene="mvk"
                     /locus_tag="VYN66_001565"
     CDS             complement(8540..9418)
                     /gene="mvk"
                     /locus_tag="VYN66_001565"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV1495563.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITETCVRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(9540..9719)
                     /locus_tag="VYN66_001566"
     CDS             complement(9540..9719)
                     /locus_tag="VYN66_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV1495564.1"
                     /translation="MLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNT
                     TVDGYFVNYNGEWVQ"
BASE COUNT         2897 a         2360 c         1768 g         2980 t
ORIGIN      
        1 gtttgagggc agtacagaaa aaccttgata ctatgcgttt tttgattgtg aagactttta
       61 tccacctatt atacaaagtt aagtatccat cacagtttac aattagcctt cattacttaa
      121 cccattcacc attatagttt aagtagtaac cacctactgt ggtattaaca gctaaagcac
      181 ctgaatcata ggcatagtac cagttaccat tgacttggaa ccagcctgtt ttcatatctc
      241 cattacttgc atttagatag taccaagtcg aaccttcctg ataccaacca gttgccatag
      301 ctcctgatga acggagatag taccatttgt tcccaaggtt ttgccaacca gttttcatat
      361 cgccattttc agcatctaaa tagtaccaag ttgaaccatc ttgataccag ccagttgcca
      421 tagctcctga tgaacggaga tagtaccatt tattaccaag atgttgccaa cctgtttgca
      481 tgatgccagt tgctggatct agatagtacc atggagctgc ttttagcttc tcttcatcat
      541 aggtaagagg gtaatcctta acccaaccac gtgctagctc tccaaccgtc aatctgttga
      601 tgcgagaatc aaagccacca tccttctgca aataatacca gtccccatca tcataaaccc
      661 aaccagtttt taaagtatga taactacgct gatcttcaaa ataatagact cgtttctctg
      721 ctgggctatc atattcttcc ccatgatgtt tgttggtatt tgtagcagtt tttgcttcta
      781 acacttgctt gccaacaaat tcttgtagta ccccatcttg accaaagtaa aaccaatctg
      841 gtctaaaagc aatctcttgt cttggagaag gaccaatcgt aacccccttg tgtggagcag
      901 gtatatattg ccagccgaca accatctctc cggataaagg atcaaaataa tagtatttcc
      961 catctatcac tctccagtag gtttctttta gatccccctt tttgtagtag gttctaccat
     1021 tttcttggac aaactgccat ccttcagaat catctgcaaa tactgtactg gtccctagca
     1081 aaccgaagaa aaatgtggct agtgcgactt gcatcatttt tttcaaaagc ttcattttat
     1141 atatcctcca acattaaatc cactcaccag atgaagcgaa gttataaact ttaccatcga
     1201 tagtttggct acctgtaacc attgctccat ttgaattgag ataataccat tttccattta
     1261 ctttttgcca atctgtaagc atcttaccgg aactgtctaa atgataccaa gtatcattta
     1321 tctcctgcca acccgtttgt ttatcatgtt gtctataatg gtaccaacta ccatttattt
     1381 tcttccatcc ttcaagagag taacctttaa gaaccgaata aataaatggc aaatttcgtt
     1441 catttaattt aactgcactg ttaatttgat tagctccatc tcctaaagta tgcactccta
     1501 ctacacggtg actagcatca taaactgtag atccactaga cccctctaaa gtatcaactt
     1561 ggtaatccaa gaacatgcca tcatcactta aaacttgttt tttatctgta tacatttgat
     1621 gaactttaaa attatatgat ggatagcctg tgatagtcac agttattcct gtcaaatttt
     1681 tttgactagt aggaagaccc aaagtcccta attttgcacc aatgggctct tctagaatta
     1741 ataaagccaa gtcatattcc cttgcatcct tagaatttaa atttctaaat tccttcaaat
     1801 aacgaacttc ctttactttg atctttccaa atggttcttg acttggacta acagccggaa
     1861 gaacataaat atcatccgct tctttcccat agtcatgtct gtagtagctg tgagccgctg
     1921 taattaacac attatcttta ataaaattcg cacttcccct cccttctaca ttcattccat
     1981 cggctactcc ataatactta gataaaacca tagctgaagt cgaatacgga tactgtaaag
     2041 tattatcaat tttaatcaaa tcatcattac cgataatact tctgattgct tttggtagta
     2101 tgaaccatat gttggtgaaa tctcagataa tgaagaatca ttagatcctg gacctttttc
     2161 tagtgtctca cttacctcat attcttcacc cgtactagaa ataacactca aagcagatac
     2221 tgtcgataac tggctagcca ataaagtact cgcaataatt gaaataccca attttttata
     2281 aacagttttc ttcattattg tatcctccta atgtaattat agcgtactat tctaaatttc
     2341 ttaatctact atagaatcaa gaaatctacc accttcttta aataccctcc attatcacat
     2401 aaacaggtaa acttttcaat taatgactgc gcttttcaat cacgctagag gtacttactt
     2461 gcttctttga tactaagttc agccattctt tccttgtttt tctcaataaa gcatgttacc
     2521 caagtgggat tcgttttgga gtagtctcgc agagtccagc caatggcttt attgataaaa
     2581 aattctgttt ggttcaagtt atgaaggaga atcttttcca ttaattgagt attggtcttc
     2641 tcttttctta acaactggtt gtcaatagcg acacgtctca gccagatatt atctgatagg
     2701 ctccatttta tactcaatga aaatcaaaga gcaaactagg aagctagccg cagattgctc
     2761 aaaacactgt tttgaggttg cagatagagc tgacgtggtt tgaagagatt ttcgaagaat
     2821 attaagatta tttcttctag ttcaggatgt ttatacacca aactccctac tactcgatct
     2881 aggatatcta ccgtgtccca ccaggatttt gtcacgacta actgctctag cttaggcaaa
     2941 tcggtttcct ttagataaga ctgcattgct ttcaaatagt tagcagccac atattggtat
     3001 tttctaggct ccttttccca gcaagtgtct gcaaaatccc aatcgataat ctttgttttt
     3061 ttcgcttctg gaaaatattt ttatagagtt tatttctttc aacctagaaa agaaaattga
     3121 tggcacatat aggcttccat gggccttgct tttttagagt cttttgctgc ttctagctcc
     3181 tcaagtaaat ctgctaaact catctaaaac tcctcatgcc ccaccaaatg gtgctgaaag
     3241 gcatagacag ccgcctgggt acgatcgcta acttcaagtt tggcaagaat gttggacacg
     3301 tgggtcttga ccgtcttgag agagataaag aggtcatctg cgatgcgctg attttcgtag
     3361 cccttggcga tgagttggag aacatctcgc tcacgcgcag tcaattcttc atgaagttcc
     3421 atatgattgc ggtggtattc aaccttcttg ctaacctctt gctcaatggc cagctcgcca
     3481 gcagctacct tactgacggc atgaagcaat tcatctgcac tagaagtctt gagcatatag
     3541 cctttggcac cagcatctaa gactggcatg attttttcat tgtccaaata agaggtcaca
     3601 atcaaaatct tggcttcagg ccattcttta aggattgcta aggtcgcgtc aatcccattc
     3661 atctcaggca tgacaatatc catgacaatg acatctggac gcagttccaa ggccaagtca
     3721 atcccttgag acccgttgga cgcctcaccc acaacttcta catcgtcttg gaggtcaaag
     3781 tagcttttca agcccaatcg gaccatttca tggtcatcta ctagtaaaat tttcatcttt
     3841 actcctttat cattccttat ctaacagggg aatacggata tcaaccgcca gcccttgctt
     3901 gggagctgtc aagagttgaa ctgttccagc catatcttca acccgctcct tgatatttcg
     3961 cagtccataa ctcaagtcgt ctaagctccc taactggaaa ccaatcccat tgtccaccac
     4021 cttcagttgc aattcaacat ctgtctgata gaggtagaca tctaggcaag atgcctgggc
     4081 atggcggagg gtattgctaa tcaactcttg caggatacgg aagatatgct cctcgatttt
     4141 cttaggcaat ttcgtcatat tctgcttgag actaacccta agatcactct tgtcctcaag
     4201 ctcttttaag agaatctgaa tcccttctat caagctcttc tgctccagtt caactggtcg
     4261 caaatgcaag agcaaaaccc gcaaatcctt ctgggctgtt tctaaaatag ctgtgacact
     4321 ctgcaactgg atctgcatct tttctctatc caatttcaaa gcctgctgac tgatacccga
     4381 taaaatcatg tgggccgcaa acaactcctg actgactgta tcgtgcaaat cccgagcaat
     4441 tcgcttccgt tccttctcga tgatttcctc ttcctgagca aggctctgat tttcagcttt
     4501 ttgaagagct tctgtcaaaa ggttaagttt acctgataag gacttgaaac tggcatccaa
     4561 atctggatct gcaacctgaa ccacttcttg ccctgccaat aaacgcttga gattagcctg
     4621 catttttctt agagaaagct cttcgatccc tcgccaaaac agggctaaga gacaggttat
     4681 ggacatgctg aaaaccaaca ataaaaagac aaatttttct gttttttcga catcgtgcaa
     4741 aaagatagac cagtcaaaat caagtatttc cagcaagctg tgggagaaaa aaaagacaaa
     4801 caggaaggag gtgagagcaa taatgacata ggcttgtttt ttcatcctct aaccacctcc
     4861 acatcaccaa tcatagtggt caagaaaatc ttgacactct tgttactctt gagatagtct
     4921 tttgtttctt gatgatagtg ttcattgcgg agggctcgct tgggctggtt gaaaaaagtc
     4981 aaatccccat agagacagtt aacgctgaga ctgacttcca catctacagg tacgatgatt
     5041 ttggtcgttc ctaccatctt tctgaggata atgacattgt catgattggt taagatgacc
     5101 ctctccagat gaatagtgtc cttgcccatg aagcgaaaga gattgatatc atcgaattgg
     5161 caagtctggt agcttgaaaa atgatgaaga tttccaaacc aacgattttt ctccttctta
     5221 accgtcacga cctcttcaaa aaccaaattg gtctgctctt tttcctggtt catcatcggg
     5281 taaagaagaa agaggctata gataaccgca acaaaaatag ctagaatcac aaaaggattg
     5341 agcataacga tgaaaaagaa gagaatggtt gccgctacta aaagaagatt atttccctct
     5401 ttaccagtgt agtagcgaat caaaagcaaa aagaggaata gtatcagcag aaaacgcgaa
     5461 aaatgctctg ataccatcaa aatcagagct cctgtcagaa gacaggcttc gataaataaa
     5521 aagattttaa attttctcat aggttcatcc tctcccttct attttatcac aattcaaaaa
     5581 agtcacctca gtctgaggat ggaaaaaagg cgctggttac gcctttttca tctgatcctt
     5641 tgcttctttt aattttccat aaagaagata gtctactttt tgtagatctg ctatggtggc
     5701 acagttaagg gaacacataa tcaagcgtag gtctgctttc cagccttgga caatgccaat
     5761 cacttcttca actgtgtagg tttcaaccaa ttccagaacg gttcgtgaca atcccacaga
     5821 cttagcacca aaaaccaagc acttaatcat atccagcgga ttccgaaccc ctccactaac
     5881 caagagttcg accttatctt tccattcttg ggcattgaga agggcctgca tggtagactg
     5941 accccattga ttgaggtaat cacgctggcc actacgacgg ttttcgatat aggcaaagct
     6001 ggtgccacca cgacccgata ggtccactgt acgaacaccg aattcatagg ctctttcgat
     6061 tgtcttggca tccattccaa agcccacttc cttgaggaca ataggaacgg gaatttgctc
     6121 gctataatct gctagatgcg attgccagct tctaaacttc ctttctccct cgggcatgag
     6181 taattcctgc atgacattga catgcacttg caatagaaca ggattcatct cttctacagt
     6241 ctgaagtcct aactcgacag gcttgtccaa tccaatattg gttccaagga ggagattggg
     6301 atgactagac ttgacagaaa aagaatcatc cgttggattt ttgagggctg cgctataaga
     6361 acccgttaca aataaaatac cacaggattc cgccacctga gccagctttt gattgatttc
     6421 tcttccctta ttacttccac cagtcatggc attgatataa aaaggaaagt cccactttcg
     6481 accagcaaac tctgtcgaaa gatcgatttc atccagattg taaagaggca aggaagaatg
     6541 aatcagctcc acctcatcaa agctattata ggaacttttc tgctcaaggg catagaggat
     6601 atgctcgtcc ttacgatttg tcgtcatgtc ctatcctttc ttgatataag agctcaatcc
     6661 ccagatcggc ccaacgattt tttaaggttt tggttgattg cgcatcaaaa ctcagggcga
     6721 tgccacagtc accaccacca gcaccactac tcttggcaac ggcctgcaaa tcttgactgg
     6781 cttctttcaa ctgtctaagc aaaggcgtgt aaatatctgt actcaagcct tctaaaagct
     6841 tgctggctac ttctacttgc tcgataatct tttctgattt cccctgttcc aaggcttcct
     6901 caaaagaaac caccgtttct tttgaggaag ttaaaaaatt ttgattgata ttttgcttga
     6961 tttgctggac catgtgactc gatacagcca cttccttggt ccatcccact aagaaatcac
     7021 attctaaagt tggtttcact tgtgaaattg aaaagcccca atcacgctcc agaactgtcg
     7081 ccaagttttc ttcttctaac caagcagcca ccttctggcg atcaaatgac tggtagagaa
     7141 ccaaatcctc tgccacaata caggcaaggt cgcccatgga accattgtct cctcgcttga
     7201 gcaagacagc gctagtcagc ttgaacaaga gctcctgatc aacagaaaca tcatacagag
     7261 ccagtaaagc cttgacaacc aagacaacga cgctgccact agaacctaga ccaaactttt
     7321 tcccttctcg ttccattttg ccacagattt ctagagaaaa aggtcttaaa ttctgaccac
     7381 gaacagcgag gaagtctccc atcaaagcaa tcgtttcttg aatcaagctg tagtcaggat
     7441 taggccttaa gtccactgcg aaatcaaaca tatctgaata gatacggtag ctgtcagaaa
     7501 aagcaatctc agccctcata tagatgagaa tatcctttat caaagctaac tgccctggct
     7561 ctaaaatagc atattcacct gcccaataga gttttccgca agttttaaca gcaatcatct
     7621 tgactcaaat cctttgtttt tgacacaatc aagcgataac gctgaccgaa aatttctgac
     7681 aaatactcca agtctttctc ctgacagaag accttaacat tgggaccagc atccatggta
     7741 aagtagcagg cctctccttt ctcacgaagc tggcgaacaa agtccatagc ctcataagag
     7801 gcatccgtca gataagaaaa ggctggacta gcagtctttg tcgtagcatg catagccagg
     7861 gcatttttct ccgttaattc tccaatcttg gcaaaatcat tttccttgag ataaatcagc
     7921 atatcctgat agtccttctc agactgacga acccagtcgt cgaaagtcgt cgaggtttcc
     7981 acacaaagtt tcatcccgtc acggctagag attggttttt tcttgtcctc tagcaccaac
     8041 ataatcatag ctagtttcaa gtctgtctct acagggtaaa tttctccact atccttatcc
     8101 caggctccta gtggtccata aaaactccga gaagaagagc ctgaggcaaa tttggcttcc
     8161 tgtgccaact gacttctatc caatccaagc ttgaaataag cattacaagc cttgaccagg
     8221 gcggacaaac cactagaact tgaggacagg cccgctgccg taggcatatt gttttgagta
     8281 tcgatacgga caaagccctc accagctgga cgataacggt caataatctt actcatcttg
     8341 gcatgctcga cctcattttg tagctgacca ttgatgtaaa attcgtcagc tgttacattg
     8401 gctggtaaag gcgacaaggt cgtctctgta tacatatttt ccaaagttag agaaatactg
     8461 ctagtagcag gcaccatctc tttttctttt ttctttcccc aatatttgat aatagcaata
     8521 tttgcgtagg aacgtactgt tacaggctct ctatccatgt ctgaacagct cctttctctt
     8581 ctaatctttc tgctagttct tgtgcgtgtg tcaaattggt taccaaggct atgatacaac
     8641 ctcctagccc accaccgctc atcttggcac ccagagcacc atggctaaga gtcgtttcaa
     8701 ccaaaaagtc tgcctcaggg ctactgactc caatttcttt taaatgtaaa tgcgcttgac
     8761 tgaggatttg tcccagtcct tcagcatctt tttgtgaaat cgcaacttct gcttgctggg
     8821 ttaattctcc caaggcatgc aaaaacggta gggcatcctt gcccttattt tgaaccactt
     8881 ggatggcttc acgagtatga ccataaacac ccgtatcggc aatcaccaaa taggcggata
     8941 aatccatctc aagttctgta aatcctacgt tcttgataaa gcgaataggt tggtcactga
     9001 gacaggtctt agcatccaaa ccactaggat tcatatgagc aatcatctca gctcgattga
     9061 ccaagatttc tagtacatca tgaggcagat cagcctgata gtagtcaaat accgcacgaa
     9121 tggccgctat gctgatagcc gctgacgaac ccatcccccg tttctcaggg atagccgagt
     9181 caatctcaca acgaacgcag gtttctgtga tatccaaata ctccagtgaa gcataaaccg
     9241 ccatggacaa ggtatcctcc tcataaaggc gccaaggact ctctgcagga actaccttac
     9301 aggtcacctc cacctccaaa agaggcaggg aaatggcagg ataaccgtaa acgaccgcat
     9361 gttcccctat taaaattatc ttactatgtg cctgaccgac accaactttt tttgtcattt
     9421 tttcctttta ctagacgaaa aaacgtctta tttttcatac aagtattaat tctttcctat
     9481 ctattttatt atattttcac aaaaaaagcg attgtttcca ttcacaatcg cttctttcat
     9541 tattgaaccc attcgccatt ataattgaca aaatagccat ctacggtcgt attcactgcc
     9601 aaggcacctg agctataagc gtagtaccat ctgccattga cctggaacca acctgtcgtc
     9661 atagaacgac gaaagaaact ccataccatt aagtaaagag gaaagtcgtg agggagcatg
     9721 cgccattgac aacctgtttt agtgacgtac aaagtctcat taacaagtac tcgtttcggt
     9781 catttatagg tgcggtgttt ggagaaatag ggttcaatct tagcccattc ttgatcgttt
     9841 aaatcagtat catatacttt gcgtgtcata actctagttt aacatttttt gtgaatacag
     9901 gttctaaata atcgaccacg aaaatttctt aagtggaaaa cgccttatga agtatgctac
     9961 gggaaagtta tgcacttaat ttgacaattc aagatgtaaa aatat
//
LOCUS       DAVLVF010000068         9894 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVLVF010000068 DAVLVF010000000
VERSION     DAVLVF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9894
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.068"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            157..5067
                     /locus_tag="VYN66_001567"
     CDS             157..5067
                     /locus_tag="VYN66_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV1495565.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEAASAAKPEEKAGEVVAETPS
                     AEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKTLKPKEIKFNS
                     WEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSNTNSKAKDHAS
                     VGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLFFNWSNSIADQ
                     ERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLGEKMRQFMLYS
                     KEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPADNFFANFNWD
                     KAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGKLRLSLGLFAP
                     DTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADRTPAVGNTFTT
                     SFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAEYDFTDAYNGG
                     NSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAFSTTPDYKFDD
                     ADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYRFNLGQLTISDNHQ
                     EPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKLLTGSSSTTIY
                     LPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFEWGMTVQDTSLPRPLAENIVPG
                     ATVIGSTFPDTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKPRTVVRWVMDH
                     AGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLDKPITAQDWRL
                     NVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQVGFADVPAGA
                     TITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGKEISNVLAVSV
                     PKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIRLTHAGVSVSG
                     FDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEPKKDYLVGDSL
                     DLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGHEVNFDVLVSP
                     KAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLKDHETSTQDQV
                     NDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSGSALAPLLEKNK
                     ALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSNKEKTVIKGLK
                     VERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEKDKKVKKVFFL
                     PEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNTVLPKPTYQPA
                     SDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVFEVDENGQRRL
                     RSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQLPNTGTADANE
                     ALIAGLASLGLASLALTLRRKREDKD"
     gene            5157..6353
                     /locus_tag="VYN66_001568"
     CDS             5157..6353
                     /locus_tag="VYN66_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV1495566.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     VALTEK"
     gene            6489..7016
                     /locus_tag="VYN66_001569"
     CDS             6489..7016
                     /locus_tag="VYN66_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1495567.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            7093..7449
                     /gene="glnR"
                     /locus_tag="VYN66_001570"
     CDS             7093..7449
                     /gene="glnR"
                     /locus_tag="VYN66_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV1495568.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYENQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            7486..8832
                     /gene="glnA"
                     /locus_tag="VYN66_001571"
     CDS             7486..8832
                     /gene="glnA"
                     /locus_tag="VYN66_001571"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV1495569.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(8928..9047)
                     /locus_tag="VYN66_001572"
     CDS             complement(8928..9047)
                     /locus_tag="VYN66_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV1495570.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            9535..9894
                     /locus_tag="VYN66_001573"
                     /pseudo
     CDS             9535..9894
                     /locus_tag="VYN66_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3191 a         1785 c         2154 g         2764 t
ORIGIN      
        1 aaccttattt tagatttgtg caaacagtta ttgttttttg tctaaaaatg ttttaggata
       61 aaaatgtgat tatcatggtt ctagagtttg ataaaaaact acagaatata aagcgctttc
      121 tttaaaacaa caaaatcaat cttttaggag gagaaaatga agaatccatt ttttgaaaga
      181 cgttgtcgtt acagtattcg taagttatca gtaggagcct gctcgctgat gattggtgct
      241 gttttatttg ctggtccagc cttggctgaa gaaactgcag ttcctgaaaa tagcggagct
      301 aatacagagc ttgtttcagg agagagtgag cattcgacca atgaagctga taagcagaat
      361 gaaggggaac atgctagaga aaacaagcta gaaaaggcag aagcagctag cgcagctaaa
      421 ccagaggaaa aagcaggtga ggtggttgca gaaacaccat ctgcagaagc aaaacctaag
      481 tctgacaagg aaacagaagc aaagcccgaa gcaactaacc aaggggatga gtctaagcca
      541 gcagcagaag ctaataagac tgaaaaagaa gtccagccag atgtccctaa aaatacagaa
      601 aaaacattaa aaccaaagga aatcaaattt aattcttggg aagaattgtt aaaatgggaa
      661 ccaggtgctc gtgaagatga tgctattaac cgcggatctg ttgtcctcgc ttcacgtcgg
      721 acaggtcatt tagtcaatga aaaagctagc aaggaagcaa aagttcaagc cttatcaaac
      781 accaattcta aagcaaaaga ccatgcttct gttggtggag aagagttcaa ggcctatgct
      841 tttgactatt ggcaatatct agattcaatg gtcttctggg aaggtctcgt accaactcct
      901 gacgttattg atgcaggtca ccgtaacggg gttcctgtat acggtacact cttcttcaac
      961 tggtctaata gtattgcaga tcaagaaaga tttgctgaag ctttgaagca agacgcagat
     1021 ggtagcttcc caattgcccg taaattggta gacatggcca agtattatgg ctatgatggc
     1081 tatttcatca accaagaaac aactggagat ttggttaaac ctcttggaga aaagatgcgc
     1141 cagtttatgc tctatagcaa ggaatatgct gctaaggtaa accatccaat caagtattct
     1201 tggtatgatg ccatgaccta taactatgga cgttaccatc aagatggttt gggagaatac
     1261 aactaccaat tcatgcaacc agaaggagat aaggttccgg cagataactt ctttgctaac
     1321 tttaactggg ataaggctaa aaatgattac actattgcaa ctgccaactg gattggtcgt
     1381 aatccttatg atgtatttgc aggtttggaa ttgcaacagg gtggttccta caagacaaag
     1441 gttaagtgga atgacatttt agacgaaaat gggaaattgc gcctttctct tggtttattt
     1501 gccccagata ccattacaag tttaggaaaa actggtgaag attatcataa aaatgaagat
     1561 atcttcttta caggttatca aggagaccct actggtcaaa aaccaggtga caaagattgg
     1621 tatggtattg ctaacctagt tgcggaccgt acgccagcgg taggtaatac ttttactact
     1681 tcttttaata caggtcatgg taaaaaatgg ttcgtagatg gtaaggtttc taaggattct
     1741 gagtggaatt atcgttcagt atcaggtgtt cttccaacat ggcgctggtg gcagacttca
     1801 acaggggaaa aacttcgtgc agaatatgat tttacagatg cctataatgg cggaaattcc
     1861 cttaaattct ctggtgatgt agccggtaag acagatcagg atgtgagact ttattctact
     1921 aagttagaag taactgagaa gaccaaactt cgtgttgccc acaagggagg aaaaggttct
     1981 aaagtttata tggcattctc tacaactcca gactacaaat tcgatgatgc agatgcatgg
     2041 aaagagctaa ccctttctga caactggaca aatgaagaat ttgatctcag ctcactagca
     2101 ggtaaaacca tctatgcagt caaactattt ttcgagcatg aaggtgctgt aaaagattat
     2161 cggttcaacc taggacaatt aactatctcg gacaatcacc aagagccaca atcgccgaca
     2221 agcttttctg tagtgaaaca atctcttaaa aatgcccaag aagcggaagc agttgtgcaa
     2281 tttaaaggca acaaggatgc agatttctat gaagtttatg aaaaagatgg agacagctgg
     2341 aaattactaa ctggctcatc ttctacaact atttatctac caaaagttag ccgctcagca
     2401 agtgctcagg gtacaactca agaactgaag gttgtagcag tcggtaaaaa tggagttcgt
     2461 tcagaagctg caaccacaac ctttgagtgg gggatgactg tccaagacac aagtcttcca
     2521 agacctctag cagaaaacat cgttccaggg gctactgtta tcggaagtac cttcccagat
     2581 acagaaggtg gagaaggtat tgaaggtatg ttgaacggta ccattactag cttgtcagat
     2641 aaatggtctt cagctcagtt gagtggtagt gtggatattc gtttgaccaa gccacgtacc
     2701 gttgttagat gggtcatgga tcatgcaggg gctggtggtg agtctgttaa cgatggcttg
     2761 atgaacacca aagactttga cctttattat aaagatgcag atggtgagtg gaagctagct
     2821 aaggaagtcc gtggcaacaa agcacacgtg acagatatca ctcttgataa accaatcact
     2881 gctcaagact ggcgcttgaa tgttgtcact tctgacaatg gaactccatg gaaggctatt
     2941 cgtatctata actggaaaat gtatgaaaag cttgatactg agagtgtcaa tattccgatg
     3001 gccaaggctg cagcccgttc tctaggcaat aacaaggtac aagttggctt tgcagatgta
     3061 ccggctggag caactattac cgtttatgat aatccaaatt ctcaaactcc actcgcaacc
     3121 ttgaagagcg aagttggagg agacctagca agtgcaccat tggatttgac aaatcaatct
     3181 ggtcttcttt attatcgtac ccagttgcca ggcaaggaaa ttagtaatgt cctagcagtt
     3241 tccgttccaa aagatgacag aagaatcaag tcagtcagcc tagaaacagg acctaagaaa
     3301 acaagctacg ccgaagggga ggatttggac cttagaggtg gtgttcttcg agttcagtat
     3361 gaaggaggaa ctgaggacga actcattcgc ctaactcacg caggtgtatc agtatcaggt
     3421 tttgatacgc atcataaggg agaacagaat cttactctcc aatatttggg acaaccagta
     3481 aatgctaatt tgtcagtgac tgtcactggc caagacgaag caagtccgaa aactattttg
     3541 ggaattgaag taagtcagga accgaaaaaa gattacctag ttggtgatag cttagacttg
     3601 tctgaaggac gctttgcagt ggcttatagc aatgacacca tggaagaaca ttcctttact
     3661 gatgagggag ttgaaatttc tggttacgat gctcaaaaga ctggtcgtca aaccttgacg
     3721 cttcgttacc aaggtcatga agtcaacttt gatgttttgg tatctccaaa agcagcattg
     3781 aacgatgagt acctcaaaca aaaattagca gaagttgaag ctgctaagaa caaggtggtc
     3841 tataactttg cttcaccaga agtaaaagaa gctttcttga aagcaattga agcggccgaa
     3901 caagtgttga aagaccatga aactagcacc caagatcaag tcaatgaccg acttaataaa
     3961 ttgacagaag ctcataaagc tctgaatggt caagagaaat ttaaggaaga aaagacagag
     4021 cttgatcgtt taacaggtga ggttcaagaa ctcttggctg ccaaaccaaa ccatccttca
     4081 ggttctgccc tagctccgct tcttgagaaa aacaaggcct tggttgaaaa agtagatttg
     4141 agtccagaag agcttgcaac agcgaaacag agtctaaaag atctggttgc tttattgaaa
     4201 gaagacaagc cagcagtctt ttctgatagt aaaacaggtg ttgaagtaca cttctcaaat
     4261 aaagagaaga ctgtcatcaa gggtttgaaa gtagagcgtg ttcaagcaag tgctgaagag
     4321 aagaaatact ttgctggaga agatgctcat gtctttgaaa tagaaggttt ggatgaaaaa
     4381 ggtcaagatg ttgatctctc ttatgcttct attgtgaaaa tcccaattga aaaagataag
     4441 aaagttaaga aagtattttt cttacctgaa ggcaaagagg cagtagaatt ggcttttgaa
     4501 caaacggata gtcatgttat ctttacagca cctcacttta ctcattatgc ctttgtttat
     4561 gaatctgctg aaaaaccaca acctgctaaa ccagcaccac aaaacacagt ccttccaaaa
     4621 cctacttatc aaccggcttc tgatcaacaa aaggctccta aattggaagt tcaagaggaa
     4681 aaggttgcct ttcatcgtca agagcatgaa aatgctgaga tgctagttgg ggaacaacga
     4741 gtcatcatac agggacgaga tggactgtta agacatgtct ttgaagttga tgaaaacggt
     4801 cagcgtcgtc ttcgttcaac agaagtcatc caagaagcga ttccagaaat tgttgaaatt
     4861 ggaacaaaag taaaaacagt accagcagta gtagctacac aggaaaaacc agctcaaaat
     4921 acagcagtta aatcagaaga agcaagcaaa caattgccaa atacaggaac agctgatgct
     4981 aatgaagccc taatagcagg cttagccagc cttggtcttg ctagtttagc cttgaccttg
     5041 agacggaaaa gagaagataa agattaaata tcgaaaaatc ttgtgaaatc tttccttata
     5101 tttctaaagt gtgatataat agtttcgaat aaaataaata aaggagtttt tgtaacatgg
     5161 caaaacttac tgttaaagac gttgacttga aaggtaaaaa agtcctcgtt cgtgttgact
     5221 tcaacgtacc attgaaagat ggtgtaatca ctaacgataa ccgtatcaca gcagctcttc
     5281 caactattaa gtacatcatc gaacaaggtg gacgtgcaat tcttttctct caccttggac
     5341 gtgtgaaaga agaagctgat aaagctggta aatcacttgc tcctgtagca gctgacttgg
     5401 cagcaaaact tggtcaagat gttgttttcc caggtgtcac tcgtggtgct gaattggaag
     5461 cggcaatcaa cgctcttgaa gatggacaag ttctcttggt tgaaaacact cgttacgaag
     5521 atgttgacgg caagaaagaa tctaaaaacg atcctgaact tggtaaatac tgggcatcac
     5581 ttggagatgg tatcttcgta aacgatgcat tcggtacagc tcaccgtgca cacgcatcta
     5641 acgttggtat ctcagcaaac gttgaaaaag cagttgctgg tttccttctt gaaaacgaaa
     5701 ttgcctacat ccaagaagca gttgaaactc cagaacgtcc attcgtggct atccttggtg
     5761 gttcaaaagt ttcagacaag atcggtgtta tcgaaaactt gcttgaaaaa gctgataaag
     5821 tccttatcgg tggtgggatg acttacacat tctacaaagc acaaggtatc gaaatcggta
     5881 actcacttgt agaagaagac aaattggatg ttgcgaaagc tcttcttgaa aaagcaaatg
     5941 gtaaattgat cttgccagtt gactcaaaag aagctaacgc atttgctggt tacactgaag
     6001 tgcgtgacac tgaaggtgaa gcagtttctg aaggcttcct tggtcttgac atcggtccaa
     6061 aatctatcgc caaatttgac gaagctttga ctggtgccaa aacagttgta tggaacggac
     6121 ctatgggtgt atttgaaaac ccagatttcc aagctggtac aatcggtgtg atggacgcta
     6181 tcgtgaaaca accaggagtt aaatcaatca tcggtggtgg tgactcagct gccgcagcga
     6241 ttaaccttgg ccgtgcagac aagttctcat ggattagtac gggtggtgga gcatcaatgg
     6301 aacttcttga aggtaaggtt cttccaggac ttgtagcctt gacagaaaaa taagatttta
     6361 taaacaaatc aaagaagaga gggatgaaag ttcctctttt cttttgctta aaataaaaac
     6421 gcttcctctc aactattact cataaaaatc accgatttat gataaaatgg aaatagaaag
     6481 ttgagattat gagttatttt aaaaaatata aattcgataa atcccagttc aaacttggta
     6541 tgcgaacctt taaaacaggt attgctgttt ttctagttct cttgattttt ggcttttttg
     6601 gctggaaagg tcttcaaatt ggtgctttga cagccgtttt tagcctgagg gagagttttg
     6661 atgagagtgt tcattttggg acttcgcgta ttctaggaaa tagtatcggt ggactctatg
     6721 ccttggtctt cttcttatta aatacctttt tccacgaagc cttttgggtg accttggtag
     6781 ttgttccaat ctgcaccatg ttaaccatta tgacaaatgt agccatgaat aacaaagcag
     6841 gggttattgg tggtgtagca gctatgttaa ttattaccct atcaattcca agtggtgaga
     6901 caattttgta cgtgtttgtg cgtgtattag aaacatttat gggagttttt gtcgcaatta
     6961 tcgtaaatta cgatattgat cgtattcgtc tctttttaga gaaaaaagaa aaataatgtt
     7021 acattttata acattattaa ttgacgtttg tcttttttta gactataata gacagaaaga
     7081 aggaaattgt aaatgaagga aaaagaattt cgccgaaata tggctgtttt tcctatcggc
     7141 agtgttatga agttgaccga tctatcggcg cgtcagattc gttattatga aaatcaagag
     7201 ttgatcaagc ccgatcgaaa cgaaggaaat cgtcgcatgt attccttgaa tgacatggat
     7261 cgtctgcttg aaatcaaaga ttatatctct gaaggttata atatcgctgc cattaagaaa
     7321 aaatatgctg aacgtgaagc gaaatccaag aaagcggtta gtcagactga agtacgtcgt
     7381 gcacttcaca atgaactcct ccaacagggg cgttttgctt cagtacagtc accttttggt
     7441 cgcggttagg caaccgcaag tagtcataga ttaaggagaa aacttatgcc aatcacagct
     7501 gcagatattc gtcgtgaagt caaggaaaaa aatgttacct ttattcgtct tatgttctca
     7561 gatattttgg gaaccatgaa aaacgtcgaa attcctgcta cagatgaaca gttagataag
     7621 gtcttgtcga acaaggttat gtttgatgga tcttctattg aaggttttgt acgtatcaat
     7681 gagtcggata tgtacttgta cccggacttg gatacatgga cagtcttccc ttggggagat
     7741 gaaaatggaa gtgttgcagg tctgatctgt gatgtttata caacagaagg tgaaccattt
     7801 gcgggtgacc ctcgtggtaa tttgaaacga gctcttcgtc acatggaaga agttggattc
     7861 aaatccttca accttggtcc agagccagaa ttcttcctat ttaagttgga tgaaaatggg
     7921 gacccaacac ttgaagtgaa tgacaagggt ggctactttg atttggcacc tactgacctt
     7981 gcggacaaca cacgtcgtga gattgtgaat gtcttgacca aaatgggatt tgaagtagaa
     8041 gcgagtcacc acgaggttgc ggttggacag catgagattg actttaagta cgatgaagtt
     8101 ctccgtgctt gtgataagat tcaaatcttt aaacttgttg ttaaaaccat tgctcgcaaa
     8161 cacggacttt acgcaacatt tatggcgaag ccaaaatttg gtattgctgg ctcaggtatg
     8221 cactgtaata tgtccttgtt tgatgcagaa ggaaataacg ccttctttga tccaaatgat
     8281 ccaaaaggaa tgcagttgtc agaaacagct taccatttcc taggcggttt gatcaagcat
     8341 gcttacaact atactgccat catgaaccca acagttaact catacaaacg tttggttcca
     8401 ggttatgaag cgcctgttta cattgcttgg gctggtcgta accgttcgcc acttgtgcgc
     8461 gtacctgctt cacgtggtat gggaactcgt cttgagttgc gttcagtgga tccaatggcg
     8521 aacccttacg ttgctatggc tgttcttttg gaagttggtt tgtatggtat tgaaaataaa
     8581 atcgaagcac cagctcctat cgaagaaaat atctacatca tgacagcaga agagcgcaag
     8641 gaagctggta ttacagacct tccatcaact cttcacaacg ctttgaaagc tttgacagaa
     8701 gatgaagtgg ttaaagctgc tctcggagat cacatctata ctagcttcct tgaagccaaa
     8761 cgaatcgaat gggcaagtta tgcaaccttc gtttcacaat gggaaattga taattattta
     8821 gacctttact aatactaata tagaagaaag attgcctgtg ggtaaacagt cacacagcag
     8881 tctaagccat caaataccta taacccttaa aatagcgatt tcggtcgcta tttatttttc
     8941 gaaaaatcct cttgactctt ttgcgaatta gatttatcat cagacgtgta gaagaaaatt
     9001 aaggagtcac tatggaaaaa ttcaatttca aaaacaatat cgggcaagaa aacaagttac
     9061 ttcaaatcga aatctacaag tttactaact tttgcaagct tcaaaattac acatctgtaa
     9121 atattttttc gaaagatata tttgaagcca tagtaaattg agactagaat agtacactac
     9181 atctgctaaa aatttctaga aactaattta actttcctaa tcgatttgtt tacatcttat
     9241 ttcaatctac tatattatct caataaatga tgatggcgac tgttttgttt gtgcttgtcg
     9301 atttttgaat cctgatcgag cggaagaata tttctgccga ggttgccaag gggaaaatcc
     9361 tgaagatatt gaattttatg atgaacaatt acaggcagaa aaggtggagg ttttgaatat
     9421 tcgattggaa gtaaaaaatt gaattatcta caatctcatc aacaataaac ctatttcaac
     9481 cccattatct ataggcgtat ttttacgcct ttttatatac ttgaagcaca taagaaacat
     9541 tatgggaagt ttgctgatgg aagtactcaa gaaattgatg ttccttatga tattcctgat
     9601 acttgggagt gggtgaggat aaaatcaatt tattggaatt ttgggcaaaa taagccagag
     9661 aaatccttta ggtatataga tacgtctagt attgatagaa aaaagaacat aatcaactac
     9721 aaaaatctac aatatctttc acctgaacaa gcgccttccc gtgctagaaa attagtttcg
     9781 cagaatagtg tcttattttc aacagttaga ccatatctaa aaaatattgc tgtagttaga
     9841 gaacttaaag agtatttgat agctagtaca gcatttattg ttttggatac ttta
//
LOCUS       DAVLVF010000069         9856 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVLVF010000069 DAVLVF010000000
VERSION     DAVLVF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9856
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.069"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            30..893
                     /locus_tag="VYN66_001574"
     CDS             30..893
                     /locus_tag="VYN66_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1495571.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERS
                     TFLTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            1276..1896
                     /locus_tag="VYN66_001575"
     CDS             1276..1896
                     /locus_tag="VYN66_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495572.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTVFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1982..2425
                     /gene="tsaE"
                     /locus_tag="VYN66_001576"
     CDS             1982..2425
                     /gene="tsaE"
                     /locus_tag="VYN66_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV1495573.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2415..2933
                     /locus_tag="VYN66_001577"
     CDS             2415..2933
                     /locus_tag="VYN66_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1495574.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGELFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2941..3957
                     /locus_tag="VYN66_001578"
     CDS             2941..3957
                     /locus_tag="VYN66_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV1495575.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4042..5298
                     /locus_tag="VYN66_001579"
     CDS             4042..5298
                     /locus_tag="VYN66_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV1495576.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGASSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQEQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5353..6519
                     /gene="recA"
                     /locus_tag="VYN66_001580"
     CDS             5353..6519
                     /gene="recA"
                     /locus_tag="VYN66_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV1495577.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            6826..8196
                     /locus_tag="VYN66_001581"
     CDS             6826..8196
                     /locus_tag="VYN66_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1495578.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            8572..9528
                     /gene="lytA"
                     /locus_tag="VYN66_001582"
     CDS             8572..9528
                     /gene="lytA"
                     /locus_tag="VYN66_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1495579.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
BASE COUNT         3017 a         1682 c         2182 g         2975 t
ORIGIN      
        1 taagcaaata ggaaggttta ttatctttaa tgaatacact cgcagagaaa ttcagattga
       61 aaagaaaaga gttgagactc tcccaacaaa ctcttgcaga aggaatttgt gaacaaagcc
      121 agattagtaa aattgaaaga ggtcatttta ttccctccgc agacttgtta ttcaaactct
      181 ctcaacgact tgaagtccct ttggattact ttttcaatga acaaatcgaa gttaagtcta
      241 acctctctaa tttcaaacat ctatctgctc gcctattaga tgacagaaat tatgacgatt
      301 tagaatatct ttataaaata gaggtagaac gaagcacttt tctaacatta gaagaccgaa
      361 cttaccttga atggattaaa tctataattg acttctatca atatgacagt aagtgtgagg
      421 caatttcgtc attggaaaat atattattaa aagtctcttc aaatactctg atttatttaa
      481 aggcgttgaa tactctatct aatttctatt ccttagtggg tcgtgaacaa gaatatgagg
      541 caaactactc tcatttaata gagttgtatc agacaaaaaa tttggaccat caagagtttt
      601 tatttggcta catcagagtt cgttacaact acgctcacta cctagtatca aaggaaaaat
      661 ataatgaagc catccaagaa gctcttgaaa cgattgaact ctgtaaacaa agacagacaa
      721 gctaccaatt ggctcctcta cttattcttg taggaaatgc tggagccaaa tttctagaca
      781 gagaacaagt caaaaattat tatatagaag caagagagtt atgtaagatt tataacaatc
      841 ctttaatgtt gatgaagata gaaaattatt tgaaggaatt agatactgtt tagttaatct
      901 tgacattata gtttttctga aacgttaaat aacctgtaag gctgatagtt agattaatac
      961 tatcagcctt ataattatgt gactctgagt ccgtctcgct ccgtgaggtg cgagacaaaa
     1021 caaccacccg tttgacggat tttcatgatt tcaaatttat attcttcgaa aatctcttca
     1081 aatcacgtca gctctatctg caatctcaaa acagtgtttt gagcaacctg tggctagcta
     1141 cctagtttga tctttgattt tcattgagta ttagtctcta gtgtttgttt agcgtcttaa
     1201 agagacattt gagaagatta gaaacagttc tctcttttct cctagtataa tgattggtag
     1261 gaagggagag agaagatgaa atcaatgaga atcttatttt tgttagcttt aattcaaatc
     1321 agtttgagta gctgtttcct atggaaggaa tgcatcttgt cctttaaaca aagtacagtt
     1381 tttttcatcg gaagcatggt tttcgtttca ggaatctgtg ctggagtaaa ttatctttat
     1441 acccgtaagc aagaagtcca tagtgtccta gccagtaaga agtctgtgaa gcttttttac
     1501 agtatgttac tcttaattaa tttgttagga gctgttcttg ttttgtcaga taacttgttc
     1561 atcaaaaata cgctgcagca agaattagtt gactttttat tgccatcctt ctttttccta
     1621 tttgggctag atttgctgat ttttttaccc ttgaaaaaat acgtgcgcga ttttcttgct
     1681 atgctagaca gaaaaaagac agtgttggtg actattttag caacacttct tttcttaaga
     1741 aatccaatga ccattgtctc acttctgatt tatattggac tgggcttgtt ttttgcagcc
     1801 tatcttgtcc caaattcggt taagaaggaa gtttcctttt atggtcatat tttccgagat
     1861 cttgtattgg tcattgttac gctcattttc ttttagagaa agcctgtttt ttatggatgt
     1921 tatgatgata agtttagtag gaaaatagac acaaaagaaa agtttttggt ataataagag
     1981 tatgtacaca aaaaatgaag aagagttgca agccttaggg gagcgtttgg gccatctatt
     2041 agcaaagaat gatgttttaa tcttaactgg agaactgggt gcaggtaaaa cgacctttac
     2101 taaaggactt gcaaaaggat tacagatttc tcaaatgatt aaaagtccca cctatactat
     2161 cgtgagagag tatgaaggtc gacttccact ttatcaccta gatgtttatc gtattgaagg
     2221 agatgctgat tctatcgact tggatgagtt tatctttggt ggcggcgtga ctgttattga
     2281 gtggggcaat ctcttaggag atgccttgcc agatgcttat ttagaattgg aaattctaaa
     2341 agaagcagat ggacgccgtt taaattttca ggcaaagggt ttgcgtgctg agaaattgtt
     2401 agaggagctt caatatggag tatgaattgc tcattaggga agcagagccc aaagatgcag
     2461 ctgaattagt ggccttttta aatcgtgtga gtttggagac agactttacc agcctagacg
     2521 gagatggtat tctcttgact agtgaggaga tggaaatatt cctcaacaag caagctagtt
     2581 cggacaatca gataacctta cttgcattct taaatggcaa aattgctggt attgtaaata
     2641 ttacagctga ccagcgtaag agagtccgtc atattggaga actcttcatt gtgattggaa
     2701 aaagatattg gaataatggc ttgggaagtt tgttgctaga agaagcgata gagtgggcac
     2761 aagcaagtgg cattctgcgt cgtctccaac tgactgtcca aactcgtaat caagcagcag
     2821 tccatcttta tcaaaagcat ggctttgtca ttgaaggtag ccaagagcgt ggggcatata
     2881 tagaagaagg gaaatttatc gatgtttact tgatgggtaa actgataggt tagtagaaat
     2941 atggttaaaa aaattattgg aatggtgcta gctttacttt ctgtaactgt agtaggagta
     3001 ggtgtttttg cttatactat ttatcaacaa gggacagaaa ccttagctaa aacctataaa
     3061 aaaatcggtg aagaaaccaa ggttattgaa gcgactgaac ctctaaccat tctgttaatg
     3121 ggagtggaca ccggaaatgt tgaacgaact gaaacttggg tcggtagaag tgatagcatg
     3181 atcttgatga cagtgaatcc taaaacgaaa aaaacaacaa tgatgagttt agagcgggat
     3241 attctgacgc gcattgaatc agggaatggt caggctcatg aagcgaaact gaactcagca
     3301 tatgcagatg gtggagcaga gcttgctata gaaaccattc aaaaaatgat gaatatccat
     3361 attgatcgct atgtgatggt caatatgaga gggttgcaaa aactagtgga tgcagtagga
     3421 ggtattacag tcaataatat cctaggtttc ccaatttcta tcagtgacca agaagaattt
     3481 aatactattt ctatcggtgt tggggagcaa catattgggg gagaagaagc cctagtctat
     3541 gcacgaatgc gttaccaaga tcctgagggg gattatggtc gtcaaaaacg tcaacgtgaa
     3601 gttattcaaa aagtcatgga aaaagctctc agtttaaata gcgttggtca ttatcaagag
     3661 attctaaaag ctttgagtga caatatgcag accaatattg atttgtctgc aaaaagtatc
     3721 cctaacttgc taggctataa agattcattt aaaaccattg aaactcagca gttgcagggt
     3781 gaaggagaga tacttcaagg tgtttcttac cagattgttt cgagagcaca tatgttggaa
     3841 atgcaaaata tactccgacg ttctttggga caagaagaag ttactcagct tgaaaccaat
     3901 gcggttttat ttgaagattt atttggcaga gcacctgttg gtgatgaaga taattaactt
     3961 ttgatacaaa taaaaaaatc aatcgtagga aaattcctgc gattttttca aaaaaatacg
     4021 aatagatagg taggaggaaa catgaaagca gaaatcattg ctgttggaac agagattttg
     4081 acaggacaga ttgtcaacac caatgcccag tttttgtcgg aaaaactagc tgagattggg
     4141 gtagatgtat attttcagac ggctgtagga gacaatgaag ttcgtctctt gtctttgctt
     4201 gagattgcca gtcaacgtag cagtctggtg attttgacag gcggtttggg gccaactgag
     4261 gacgacctaa ccaaacaaac cctagctaaa tttttaggga aagcattagt ctttgatcct
     4321 caggctcagg agaagttgga tatctttttt gccctgcgac cagactatgc ccgaacaccg
     4381 aataacgaaa gacaagctca aattgtagaa ggagcgattc cactgccaaa cgaaacagga
     4441 ctggctgttg gaggaaaatt agaagtagac ggagtgacct atgtcgtcct tccaggtccg
     4501 ccaagtgaat tgaaacccat ggtcttaaac caacttctac ccaagttgat gacagggagc
     4561 aagctgtatt cccgagttct tcgtttcttt gggattggcg agagccagtt ggttacgatt
     4621 ttggctgatt taattgataa tcagatcgat cctaccttgg ccccttatgc caagacagga
     4681 gaagtcactc tacgtctgtc aacaaaggct agcagtcaag aagaggcgaa tcaagcgctg
     4741 gatatcttgg aaaatcaaat cttggactgc cagactttcg aaggaatttc tttacgagac
     4801 ttttgctatg gttatgggga agaaactagt ttagccagca ttgtggtaga agaactgaaa
     4861 aggcaaggga aaaccatcgc ggctgcagag agtttaacgg caggtctttt ccaagctacc
     4921 gtggcgaatt tttctggagc ttcaagtata tttaagggtg gttttgtgac ctatagcttg
     4981 gaggaaaaat caaggatgtt ggatattcct gccaagaatt tggaagaaca tggtgtggtg
     5041 tctgaattta cagctcagaa gatggctgag caggcacgaa gcaagaccca gtctgatttt
     5101 ggaattagtt tgaccggagt ggcaggacca gatagcctag aaggacaccc agttgggaca
     5161 gtcttcatag gcttggcgca agagcaagga actgaggtta tcaaggttaa tattggaggc
     5221 agaagccgag cagatgtacg tcatattgcg gttatgcatg cctttaacct agttcgcaag
     5281 gctttattaa gtgactaact tttgatataa tagtagatag gtctgaggat cattagaatg
     5341 taggagaata gaatggcgaa aaaaccaaaa aaattagaag aaatttcaaa aaaatttggg
     5401 gcagaacgtg aaaaggcctt gaatgatgct cttaaattga ttgagaaaga ctttggtaaa
     5461 ggatcaatca tgcgtttggg tgaacgtgcg gagcaaaagg tgcaagtgat gagctcaggt
     5521 tctttagctc ttgacattgc ccttggctca ggtggttatc ctaagggacg tatcatcgaa
     5581 atctatggcc cagagtcatc tggtaagaca acggttgccc ttcatgcagt tgcacaagcg
     5641 caaaaagaag gtgggattgc tgcctttatc gatgcggaac atgcccttga tccagcttat
     5701 gctgcggccc ttggtgtcaa tattgacgaa ttgctcttgt ctcaaccaga ctcaggagag
     5761 caaggtcttg agattgcggg aaaattgatt gactcaggtg cagttgatct tgtcgtagtc
     5821 gactcagttg ctgcccttgt tcctcgtgcg gaaattgatg gagatatcgg agatagccat
     5881 gttggtttgc aggctcgtat gatgagccag gccatgcgta aacttggcgc ctctatcaat
     5941 aaaaccaaaa caattgccat ttttatcaac caattgcgtg aaaaagttgg agtgatgttt
     6001 ggaaatccag aaacaacacc gggcggacgt gctttgaaat tctatgcttc agtccgcttg
     6061 gatgttcgtg gtaatacaca aattaaggga actggtgatc aaaaagaaac caatgtcggt
     6121 aaagaaacta agattaaggt tgtaaaaaat aaggtagctc caccgtttaa ggaagccgta
     6181 gttgaaatta tgtacggaga aggaatttct aagactggtg agcttttgaa gattgcaagc
     6241 gatttggata ttatcaaaaa agcaggggct tggtattctt acaaagatga aaaaattggg
     6301 caaggttctg agaatgctaa gaaatacttg gcagagcacc cagaaatctt tgatgaaatt
     6361 gataagcaag tccgttctaa atttggcttg attgatggag aagaagtttc agaacaagat
     6421 actgaaaaca aaaaagatga gccaaagaaa gaagaagcag tgaatgaaga agttccgctt
     6481 gacttaggcg atgaacttga aatcgaaatt gaagaataag ctgttaaagc agtggagaaa
     6541 tccgctactt tttcgatttt tgattcaagt ttttagatta tatatagtag cttgaaataa
     6601 gatatgaaca actttattag gaaagtcaaa ttaatttcta gaaatgtttt agcagctaca
     6661 gcgtactatt ccaaactcaa ccaactataa tagatcgaaa ctagaatagt acacatctac
     6721 ttctaaaaca ttgttaaaaa tcgatttgac tgtccttatt tcattccgct atatataagt
     6781 ttgctgtttc ttgtcgctcc tctggaaagc tgatataata gctttatgaa taaaaaacga
     6841 acagtggacc tgatacatgg tccgattctt ccctcgctct taagcttcac ctttccaatc
     6901 ttgctatcaa atatttttca acagctctat aacactgctg atgtcttgat tgttggacga
     6961 tttcttggtc aagaatcctt ggctgcagta ggagcgacga cagcgatttt tgacctgatt
     7021 gtaggcttta cacttggtgt tggcaatggc atggggattg tcattgctcg ttattatggg
     7081 gctcggaatt tcactaaaat caaggaagca gtagcagcca cctggatttt aggtgctctt
     7141 ttgagcattc tagttatgtt gctgggcttt cttggcttgt atcctctctt gcaatactta
     7201 gatactcctg cagaaattct tcctcaatct tatcaatata tttctatgat tgtgacctgt
     7261 gtaggtgtca gctttgctta taatcttttt gcaggcttgt tgcggtctat tggtgacagt
     7321 ctagcagccc tgggatttct gattttctct gccttggtta atgtggttct ggatctctat
     7381 tttattacgc aattgcatct gggagttcaa tccgcaggac ttgctaccat tatttcgcaa
     7441 ggtttatcag cggttctctg cttttattat attcgtaaaa gtgtgccaga actcttgcca
     7501 cagtttaaac atttcaaatg ggacaaaagc ttgtacgcgg atctcttgga gcaaggtttg
     7561 gctatgggct tgatgagttc aattgtatct atcggcagtg tgattttaca gttttctgtt
     7621 aatacatttg gtgcagtgat tattagtgcc cagacggcag ctcgacgcat tatgaccttt
     7681 gcccttcttc ctatgaccgc tatttctgca tcaatgacga cctttgcttc tcagaatcta
     7741 ggagctaagc gacctgaccg cattgttcaa ggtcttcgaa tcggcagtcg tttaagtata
     7801 tcctgggcag tttttgtttg tattttcctc ttttttgcca gtccagcctt ggtttccttc
     7861 ttggctagtt cgacagatgg ttacttgata gaaaatggaa gtctctatct gcaaatcagt
     7921 tcagcctttt atcccatttt gagcctcttg ttgatttatc gcaattgctt gcagggcttg
     7981 gggcagaaga tccttcctct agtttctagc tttattgaac taatcggaaa aatcgttttt
     8041 gtggttttga ttattccttg ggcaggatat aagggtgtta tcctttgtga acctcttatc
     8101 tgggttgcca tgacagttca actgtacttc tcactattcc gtcatccctt gataaaagaa
     8161 ggaaaggcaa ttttggcaac caaagtgcaa tcctagttgg atttactgaa taaaatccat
     8221 ttcctctagt gaacatcgaa aaaacttgtg ttctcttctt tagtttggtg ttgaaaatag
     8281 tttaacagac ttttgacttc ttttatatga tataataaag tatagtattt atgaaaagga
     8341 catatagaga ctgtaaaaat atacttttga aaagcttttt agtctggggt gttattgtag
     8401 atagaatgca gaccttgtca gtcctattta cagtgtcaaa atagtgcgtt ttgaggttct
     8461 atctacaagc ctaatcgtga ctaagattgt cttctttgta aggtagaaat aaaggagttt
     8521 ctggttctgg attgtaaaaa atgagttgtt ttaattgata aggagtagaa tatggaaatt
     8581 aatgtgagta aattaagaac agatttgcct caagtcggcg tgcaaccata taggcaagta
     8641 cacgcacact caactgggaa tccgcattca accgtacaga atgaagcgga ttatcactgg
     8701 cggaaagacc cagaattagg ttttttctcg cacattgttg ggaacggttg catcatgcag
     8761 gtaggacctg ttgataatgg tgcctgggac gttgggggcg gttggaatgc tgaaacctat
     8821 gcagcggttg aactgattga aagccattca actaaagaag agttcatgac ggactaccgc
     8881 ctttatatcg aactcttacg caatctagca gatgaagcag gtttgccgaa aacgcttgat
     8941 acagggagtt tagctggaat taaaacgcac gagtattgca cgaataacca accaaacaac
     9001 cactcagacc atgtggatcc atacccttac ttggcaaaat ggggcattag ccgtgagcag
     9061 tttaagtatg atattgagaa cggcttgacg attgaaacag gctggcagaa gaatgacact
     9121 ggctactggt acgtacattc agacggctct tatccaaaag acaagtttga gaaaatcaat
     9181 ggcacttggt actactttga cagttcaggc tatatgcttg cagaccgctg gaggaagcac
     9241 acagacggca actggtactg gttcgacaac tcaggcgaaa tggctacagg ctggaagaaa
     9301 atcgctgata agtggtacta tttcaacgaa gaaggtgcca tgaagacagg ctgggtcaag
     9361 tacaaggaca cttggtacta cttagacgct aaagaaggcg ccatggtatc aaatgccttt
     9421 atccagtcag cggacggaac aggctggtac tacctcaaac cagacggaac actggcagac
     9481 aagccagaat tcacagtaga gccagatggc ttgattacag taaaataata atggaatgtc
     9541 tttcaaatca gaacagcgca tattattagg tcttgaaaaa gcttaatagt atgcgttttt
     9601 ttgtggagat atttccttca atgtttgcta ctataattaa acaaaaatca gaaagcaaac
     9661 tagaaagtta tgctcaaata aaatctaaat ttgacaatgt aaaccgagtc ggatagcttt
     9721 aagtactgtt ttgaggttga agatacgatt tttgatagga actcatcaat tttagatttt
     9781 taagcagcat caataaattg cttccttgtt ttgtcataat ttttttattt aaaaaattat
     9841 gacaaaagtg tgctat
//
LOCUS       DAVLVF010000070         9758 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVLVF010000070 DAVLVF010000000
VERSION     DAVLVF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.070"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(162..1925)
                     /locus_tag="VYN66_001583"
     CDS             complement(162..1925)
                     /locus_tag="VYN66_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV1495580.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINFLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFEFMKKLTGAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASFFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
     gene            complement(1934..2524)
                     /locus_tag="VYN66_001584"
     CDS             complement(1934..2524)
                     /locus_tag="VYN66_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV1495581.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            complement(2622..3113)
                     /gene="rocS"
                     /locus_tag="VYN66_001585"
     CDS             complement(2622..3113)
                     /gene="rocS"
                     /locus_tag="VYN66_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV1495582.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            complement(3190..3882)
                     /locus_tag="VYN66_001586"
     CDS             complement(3190..3882)
                     /locus_tag="VYN66_001586"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV1495583.1"
                     /translation="MKIGIIAAMPEELAYLVQHLYNVQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHTLNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLAFLKALD"
     gene            complement(3899..4213)
                     /locus_tag="VYN66_001587"
     CDS             complement(3899..4213)
                     /locus_tag="VYN66_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV1495584.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKNHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            complement(4225..4770)
                     /locus_tag="VYN66_001588"
     CDS             complement(4225..4770)
                     /locus_tag="VYN66_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1495585.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            complement(4780..6159)
                     /gene="glmU"
                     /locus_tag="VYN66_001589"
     CDS             complement(4780..6159)
                     /gene="glmU"
                     /locus_tag="VYN66_001589"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV1495586.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            6303..7103
                     /locus_tag="VYN66_001590"
     CDS             6303..7103
                     /locus_tag="VYN66_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV1495587.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            7198..8040
                     /locus_tag="VYN66_001591"
     CDS             7198..8040
                     /locus_tag="VYN66_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV1495588.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            8040..8522
                     /locus_tag="VYN66_001592"
     CDS             8040..8522
                     /locus_tag="VYN66_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV1495589.1"
                     /translation="MAKKVKIKKTLAEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            8522..9142
                     /locus_tag="VYN66_001593"
     CDS             8522..9142
                     /locus_tag="VYN66_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1495590.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(9396..9491)
                     /locus_tag="VYN66_001594"
                     /pseudo
     CDS             complement(9396..9491)
                     /locus_tag="VYN66_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2981 a         2075 c         1689 g         3013 t
ORIGIN      
        1 agaagttata gtgtagcatt ccaacttcaa agcactataa agtaaattga aacaagaaca
       61 atacaaacaa ttctcgtaaa cggattgcaa ccacaaaaaa gcaagcattc acaagaatac
      121 ttacctatca tgggaggaac aaccgttcct cttttttatt actaaaattc aaagaattcc
      181 aatgcttttt tcaagagcaa atccgtatat tctggatctt cttgggctac ttctatttcc
      241 cgctgaactt tttccaaatc atctgtaatc actccatcta ctcctaagtg aagagatttg
      301 ctgatagctt ctgaatcatt gacagtccag acataaagtt tctgatccgt tgtccatagt
      361 ttgcttacaa aatattcatc caaggttgag tactccatag tatatcctgt cgctcttgtt
      421 ttaggaaaga cagaattgta gggcatgatg aaataaactg gcagttcggc atcatactgt
      481 cttacttttt cgacaacatg gtagtctaaa gactggattt gatgtccata aatcttgagc
      541 tttgcagcat aacgggctaa aaagcggttc atcatgtctg gactatcttt tttactggtt
      601 ttaatttcaa ttagtaattt ttgaccaagt tcgttggctc gactgagata atcttcaaag
      661 cttgaaattt tagtctggta gccattttca aaaatatcaa tccctttaag ctcctccaag
      721 tttaagtctt gaggactttt attgatacct gctagatttt tcaagttagc atcatgcatc
      781 atgacaaact gcccatcttt tgtttcctgc acgtccgtct ccaccaagtc tggtttgagt
      841 tgtgctgtag tttccaagga ctctactgta ttttgaatcc catttgcatt ggaaacccct
      901 cggtgagaaa taagttgagg tagatgaacc atgggagcct ccagataaat ataaccttct
      961 aaggcaaaga aaaaactggc acaagtcatg acaccccatc gcacgatgtg atctttttct
     1021 ctcctaggaa gcatatccag ctcctttcct gtcaaaaatg aaacaaattt aaccaaaaaa
     1081 taagtcagag ccatataata gagattttta atcacgacaa aattcaaaat accaagaatc
     1141 agagactctc tctgagtgat atcatctacc aaagtttgag ccaataataa aggaatcaaa
     1201 ggaagataaa ataataaatg tgctttgagc aagatgtaaa ataaattcca agcataaaaa
     1261 gtaactctct tcttggtttt ctccaagcta aacatcactg cttctcgaac agtcagctga
     1321 tcatatacaa tcttcggaag ggcaaacatc aatctgacag agacatagag aaagataaga
     1381 gatagaagta ggatgctcag ccaccacatc caatatttat cttctaaata agcttggata
     1441 aactctggaa tgacgatttt attaagataa taaatcttca gcattttccg tataaaagga
     1501 aacagcatag ctatatagaa aaagataaac aaggctttag cgccagttag ctttttcata
     1561 aattcaaaac tttcatggaa aaccttgcgg atatactcaa ttagccttcg cttttcatta
     1621 tagaggagat gacgagcacc aataaagagg agtcctattt gaaaataagc aaccagaaag
     1681 ttaattacaa tcaaggctaa aaaagctaga ctaatcaatg gagaatgagt aaggatggct
     1741 aagacattgt tataggaaat aaaaagataa cctgtctgat ctaataagaa gctagccaac
     1801 catgaattga atggtaccca caaatactcc actatcataa aaatcaagaa aaatagaaag
     1861 aggattttat caagattgag gtaaatctgt ttaagaccca attttttagg tttttcaggt
     1921 ttcataggca ctcctagtca aataattgag acaagtccaa gccaccaaaa ggattgtttg
     1981 ataagctact ttctgtctct aacaattccc tagcttgatc cgactctaag aaggattcgt
     2041 aaacacgcgc cgtcatccga gcatcctcta aactattatg agactgacct tgaaatccaa
     2101 gaaatgaggc aacagtttgc aatttgagat tggcaatacc atgtaaatct gaactccgac
     2161 gttcaaaagc ttcatcatac aaatccacct tgtactgttg gctatagtct aaaccatgct
     2221 ctgctaaaat aggtaaatca cttttagcag cattgtagcc tatcatcagc aaagaaccca
     2281 caaactcttg gaattcttct aagactttct caactggagg agcatctttt aaggtctcag
     2341 ctgtaatccc ggtcaaacca ttgataaagc tttttagagg gacgctggta tgaacatagg
     2401 aatcataggc atcgatttcc cgaccatctc taaaacgcac tgccgatact tgaatcaagt
     2461 gtgtttgtcc ttcgtgctga ttaaattcta aatcaaaagc aatgtaatct tctaatcgtt
     2521 ccattctgta cctctcctct actgtctctg ttctaatcaa ctatactatt cacaaacaaa
     2581 agaagccatc aggttagcat ttaacctaaa cagctccttg attatcctcc aaataaacga
     2641 gcaaaaaagc cttttttagt ggattggact tcttcttttg cttggtccag ctctagctta
     2701 agattttctt gatccttcat ggcttgaagg gtcaattgtt gttgctgatc taattgtttg
     2761 tctttctcag caatctgacg gtctttgata cgcatctgct cgtctttttc tgataactga
     2821 cgatccttgg cttttaattg ttcataaaga cgaagaattt ctgcattctt ctcatccact
     2881 agaatctcca tcagttcacg ttgtttgaca tcttcactga caggttcatc ttcaaaaatc
     2941 gtttttttat agatttcttc tagcttaatc aagccacttc tggtaacaac tgttacccct
     3001 ttgtcatttt tatctgtgtc ttcttctggt aattctttga cacggttatt gattgcttgg
     3061 cgagataatc ctaagacctc tgcaatctca ctgacggtca tttcaatact cataatatcc
     3121 tctgaaacgt tttctagctt ttctttactt aaatcttatc ataagctaga aaaactgtca
     3181 aatttccgct taatctaaag ccttcaaaaa ggccaacaag acttgggcag agcgacgtcc
     3241 agcttcgata ataaactcat caaaaaagat gtttgcttca tggttggcat tgtcactcat
     3301 agctcggatg actaagactg ggagattgag ggtatgcgct gcttgagcaa tagctgcccc
     3361 ctccatctcc acggctaaaa cttctgggaa atgggactta atcgcttcta tcttgtcatt
     3421 tcctgcaaca aaactatctc ctgtagcaat caaaccaaga tgccagtttt ggtccaattg
     3481 agataaactc tcttggattt gagcaacaaa ggttttgtct gattcgaaat aaagcggttg
     3541 ttgcgccatt tgtccataag catagccaaa agctgtgaca tccacgtcat gataggctaa
     3601 tttgtcagca atcacgacat ccccaacagc gataccttct gctactgccc cagctgaacc
     3661 cgtattaata agggcatcca cctggaaatg atcagccaaa atcgccacac tcatagcaga
     3721 catgacctta ccaattccac tttctacaag aacgacttca tgagaagcaa tggttcctgt
     3781 atgataggta ttccccaaaa caacttgctc ctggacatta tataaatgct ggaccagata
     3841 agccagttct tctggcatag cagcaataat tcctattttc atttcaattc cttttctatt
     3901 acaaaagttt cattgctaaa acaagcaaaa tcaagagaaa gatgaccgcg aataagattt
     3961 tattcaactt agaattgaag acatttctct tggtattttc aatgcgacgg cttttatgaa
     4021 tagatggttc gacctgaatc ttcaaggttt cctggctaaa accatgattc ttaggattgg
     4081 cataaccaaa acgggaagaa gaggttggta aaatcttagt ctcctcatta tcatctagca
     4141 aaggaggacc tgaaattttt tcgcctctat tagctctttc aatcatttca tccgttaata
     4201 aagatttacc catactgacc tcctctattt tttctgcaac tcccaatact gaacagccat
     4261 aattgtcttg gcatcacaaa tatgacctga ttggattaat tctttcgctt cttctaagct
     4321 cacttcaagg acttccaagg tttcatcctc atcctgcgga cgcggatttt ccacttttgt
     4381 caaatcgctt gctaaatata gttttaactt ctcattacaa aagccaatag ctgaataaaa
     4441 atcgtacaag agttctaatt tccctgtata ggctgtttct tcctctaatt cacgaagggc
     4501 agctgccaca ggggctgtgt tttctcctac ttccaatttt ccggctggaa tttcgtaaga
     4561 gacagcctcg atagctttgc ggtactgctt gaccaagatc agtttttgtt catccgttac
     4621 tgctaaaaca cagacagccc cattgtggaa aatcaaatcc cgttgggcag ttcccttgcc
     4681 ttctggtaat tcaacctgat cttggaccag tttaaatatt ggtccttgat agatttcttt
     4741 tcggctaagc gttttttctt caaactccat gataggctcc tactggttct taggatgatg
     4801 aggaagacgt gttgcatatt cgtctttatt gatctgacga ccgcgaccaa tagcaatagc
     4861 atctgctggc acgtctttag taatagttga accagcacca acgagggaat tgtcaccaag
     4921 ttctactggt gcaataatgg ttgaatttga accaacaaag acattgtctc caatgactgt
     4981 cttgtatttg tttttgccgt cataattgac tgtaatagtt ccagcaccga aattaacgtt
     5041 gcttcccact tcacagtttc cgatataagt caaatgacca gccttggtat tctcaccgat
     5101 tgaagatcct ttcacctcaa caaagttacc aatatgaact tgggcaccca gacttgaatt
     5161 tggacgaatg tgagcataag gaccgactgt cacaccgtct gcaacactac tttcctcaat
     5221 catagaattg gtaatgaccg ctcctgctcc gatagtgctg tccactacat aagtaccgtt
     5281 tgtcaaaaca gtctcagcac caattttcgt ttgccctttc aaggtaacat tggcttcgat
     5341 ttgaacttcc ggagcaatct caacatcaat atcgatataa gttgcttctg gattgacaaa
     5401 gctaacaccg ttgaccatat gtttatgatt gatgcgacga cgcataactg actcagctgt
     5461 cgcaagcgcc acacggtcat ttaccccaag actttcatca aaatctttca aagtataagc
     5521 gccaactttt tcaccagttt cacggaaaat accaatgacg tctgtaatat agtattcgcc
     5581 ttgagcgtta ttggtattga tatttttcaa agcctcaaac aaacgctcgt tgtcaaagac
     5641 gtatgttcca gtgttgattt ccttgatttg cttttcaaaa tctgtagcat ccttctgctc
     5701 aacaatacga agaacctcag cattgtcatt acgaacaatt cgtccatagc caaaaggatt
     5761 atccgtttca gcagtcaaga tagtggccac atttttatga ttgatatgga aatcaatcaa
     5821 gtttttcaag ctttcaccag tgattaaagg agtatctcct gcaatgacca aggtgtgtcc
     5881 tgacaaacct tctaagatag gttctgtcat cataactgca tgaccagttc ccaactgttc
     5941 agattgagtc acaaattctg tctgtccagc caagacctcc tcaaccaatt ctgccttgtg
     6001 tcctacaact gttactgtct tttcaggttg gatagctccc acactacgga aaacatgttc
     6061 caacatagaa atacccgcaa ccttgtgcaa aacttttggc aaatcagatt tcatgcgagt
     6121 ccctttaccc gctgctaaaa taatggcaaa atttgacata atcttctctt ttctgtagaa
     6181 attccctttt attataccat atttcaaatc attccgcact cttgaatgaa aaaatgctct
     6241 aaagtctctt ccttaaccca tttttttgat tttttttgat ttttagggta gaattataag
     6301 atatgagaaa acaaattcat ccacttattt tatttagttt tttggggatt atgatgttca
     6361 ttttaatcat caatcgccca cttaatgaca accaatcgtt taaaacaaaa tccaatatag
     6421 cacagattga agcacaggct ttgaaacact tagacaaacc gattattgac ctctctggct
     6481 ggcagcgtcc tgaagaaatc aactacgatg ccctctcaca aaatatttca ggtgctattg
     6541 ttcgtgttca cagtggtgct caaacgacaa aagaaaatga tgcttcattt atcaatggaa
     6601 tagacaaggc ctataagagt catatcactg aacttcaaaa gcggaatgtc ccagttgctg
     6661 tctacgctta tgtagctgga aaaagtgtcc aagaaatgga aaaggctgcc gaagttttct
     6721 acaatgctgc ctctccttac agccctagtt actattggct tgacgtggaa gacaaaacca
     6781 tgtctaatat gaacgaaggt gtcgaaaact ttcgagcaaa actagcatca ctaggtgcta
     6841 aaaacatcgg catctacgtt ggtgtctact tcatggaaga acacagtatt gatacaggca
     6901 agtttacctc tgtttggatt ccgtcctacg gctcagactc aggatttttg gagagcagtc
     6961 ctaaaactga tttagattat gatattcacc aatacacatc taaaggaaaa attgccggct
     7021 ttgaccacga tttagatatc aacgttatct ctcccttaaa aaataaagaa gaaacctttc
     7081 gaaaactttt tttaaaacct taaaagcatt tgtttatcat ttgctttttc tttttgtgtc
     7141 tgattgtgat acaatatagt aaggattttt tgaaatagaa atagttggag gaaatatatg
     7201 ctctcatggt tagcacgcgt tattaaaggg attgtaattg ctcttggatt tatcctaccg
     7261 ggaatttccg gaggggttct agcagcaatc ttaggaatct atgaacgaat gattggcttt
     7321 ctggcccatc cctttaaaga ctttaaagaa aatgttttgt actttattcc agttgccatc
     7381 ggtatgcttc tgggaatcgg cttattttcc tacccgattg aatacctgct tgaaaattat
     7441 caggtttttg tattatggag ctttgcggga gctattatcg gtacagttcc tagcctcctc
     7501 aaagaatcaa ctcgagaatc tgaccgagac aagattgatt tagcttggtt atggacaacc
     7561 tttatcattt ctggattagg actctatgcc ttaaattttg tcgttggaac cttaagcgcc
     7621 agctttctta acttcgtcct agcaggcgca ctattggccc ttggcgtctt ggttcctggc
     7681 ctcagcccat caaatttact tttgattttg ggactctatg ctcctatgtt gactggtttt
     7741 aaaacttttg atttcttggg aaccttcttt ccgattggaa ttggtgcagg tgcaactctc
     7801 atcgtttttt caaaattgat ggattatgcc ttaaacaact accactcacg tgtctatcat
     7861 ttcatcatcg gtatcgtcct atcaagtacc cttttgatct taattccaaa tgcaggaaac
     7921 gctgaaagta tccaatacac aggactttca cttgtcggtt atgtcatcat cgccttcttc
     7981 tttgcgctgg gaatctggct tggtatttgg atgagtcaat tggaggataa atataaataa
     8041 tggcaaaaaa agttaaaatc aaaaaaacat tggcggaaca aatcctatct aaagcagcta
     8101 tccctcatca ggggattcaa atcaatgccc tagaaggaga gcttcctcaa ggttatgaac
     8161 gagatcagat tttcaaaacc ttggcgcttt tgggagacaa gaccggaccg attatcggaa
     8221 ttgtccctat cactcagcac ttgtctgaaa agaaactagc taaaatttct ggcaataaaa
     8281 aagtgagcat gattccacaa aaggacttag aaaaaacaac tggttacatt catggagcca
     8341 ataatcctgt cggaattcgt cagaaacaca attatcccat ttttatcgat aaaattgctc
     8401 tagatttgga tcaaatgatt gtctctgcag gcgaagtcgg acacagcatt atcgtcgcgc
     8461 cacaagactt ggctagcttt gtaaaggcag actttgtaga tattttggag gacatcaagt
     8521 aatgaaactc tactttgtcc gccacggtcg taccctctgg aaccaagaag gtcgttttca
     8581 aggtgctagt ggtgattctc ctcttcttcc tgaatccatt gaaaccctaa aacgacttgg
     8641 tcagtatctc aaggaaattc cttttgatca gatttattca agtgatttac ctcgagcggt
     8701 caaatctgct gagattatcc aaagtcaact ctataccccc tgttctttag aaatcgttcc
     8761 taatctccgt gaatggcaac ttgggaagtt agaaggtttg aaaattgcaa ccttggaagc
     8821 tatttacccg caacaaatcc aggcttttcg ttcaaatctt gctcaatttg acactcgaat
     8881 gttcggagcc gaatccctct atagtacaac tcaacggacc atccaattta tcaaatcatt
     8941 aaaagatagt ccagctgaac gtattctaat tgtcggacac ggcgccaatc ttactgccag
     9001 tcttcgtact ctcctaggct ataaagaacc acttcttcgt aaagatggcg gtctagcaaa
     9061 tgccagcctg accattatag aaacccatga ttttgaaaca ttcactctca atacttggaa
     9121 tgatacttct tatcaatcat aattatagcc tagccttctg aactagaacc ataggagata
     9181 tagtcagttg aaacaaaaat aagataaaag agcctcgtaa aaaggtattg caacttgcca
     9241 ataccttttt tgatatgaac acgtgtcttc tcaacaggat tgtagagcaa aatgaaataa
     9301 gaactgaata atatgatttg agaatttaaa tcaatttata agaatgtttt agaagcagtt
     9361 atgtactatt ttagattcac ttttctatac taagatgagt agggaggaaa aggtaaaagt
     9421 ttatgcccaa actcttcaca taagaactct agcttaccca ttctatggaa tcttgcatta
     9481 tccattaaaa cttccagact catacccttc taaaatctat tcaaaccacg tcagcttcac
     9541 attatatatg ttggttacct cacaagtttt atctgcaacc tcaaagctgt tctttgagca
     9601 gcctacagct agcttcctag tttaatcttt gcttttcact gagtatcaag ttaaaaaatg
     9661 aggctggtca aaaagtcttt aaaataaaaa aaacgcatat tatcaggtgt taaatgaact
     9721 gcaccccaaa agttagacag aaaaaatcta acttttgg
//
LOCUS       DAVLVF010000071         9748 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVLVF010000071 DAVLVF010000000
VERSION     DAVLVF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9748
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.071"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(114..1784)
                     /locus_tag="VYN66_001595"
     CDS             complement(114..1784)
                     /locus_tag="VYN66_001595"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV1495591.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIYVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2014..2703
                     /gene="coaB"
                     /locus_tag="VYN66_001596"
     CDS             2014..2703
                     /gene="coaB"
                     /locus_tag="VYN66_001596"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV1495592.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2715..3266
                     /gene="coaC"
                     /locus_tag="VYN66_001597"
     CDS             2715..3266
                     /gene="coaC"
                     /locus_tag="VYN66_001597"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV1495593.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            3250..3813
                     /locus_tag="VYN66_001598"
     CDS             3250..3813
                     /locus_tag="VYN66_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1495594.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            4091..4618
                     /locus_tag="VYN66_001599"
     CDS             4091..4618
                     /locus_tag="VYN66_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1495595.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            4630..5145
                     /locus_tag="VYN66_001600"
     CDS             4630..5145
                     /locus_tag="VYN66_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV1495596.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(5142..5609)
                     /locus_tag="VYN66_001601"
     CDS             complement(5142..5609)
                     /locus_tag="VYN66_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1495597.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGSLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(5683..6171)
                     /locus_tag="VYN66_001602"
     CDS             complement(5683..6171)
                     /locus_tag="VYN66_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1495598.1"
                     /translation="MTVIIKSMETPKEIEGKSLVHWQMWREAYDDLLPAEFQETMTLE
                     RCRLFSQKYPENTLIAIGDLKVVGFISYGNFRDETMQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            complement(6137..6700)
                     /locus_tag="VYN66_001603"
     CDS             complement(6137..6700)
                     /locus_tag="VYN66_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1495599.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            complement(6710..8698)
                     /gene="uvrB"
                     /locus_tag="VYN66_001604"
     CDS             complement(6710..8698)
                     /gene="uvrB"
                     /locus_tag="VYN66_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV1495600.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(8775..9728)
                     /locus_tag="VYN66_001605"
     CDS             complement(8775..9728)
                     /locus_tag="VYN66_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1495601.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLQQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSSLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
BASE COUNT         2893 a         2195 c         1747 g         2913 t
ORIGIN      
        1 tagtttgaag agattttcga agagtataaa tcttattgat gaactgcttg cagtctgaga
       61 aaaaatgagc ttggatatta tttccaaact cacttaaagt caatttcaat ccactagaac
      121 aagcctagta cagttccatc gctttcaaca tccatgttga gagctgctgg acgttttgga
      181 agacctggca tggtcataac atcaccagtt aaggcaacga tgaagcctgc acctaatttt
      241 ggtaccaatt cacgaatggt aatttcaaag ttttctggtg ctccaagcgc atttggattg
      301 tctgagaaac tgtattgagt tttagccata cagattggca atttgtccca accgttttga
      361 acgatttgag caatttgtgt ttgagctttc ttctcaaagt tcactttgct accacgatag
      421 atttcagtga caattttttc aatcttttct tggacagaaa gggcattatc gtacaaacgt
      481 ttatagttag ctggattttc agcaattgtc ttaacaactg tttcggcaag tgctactcca
      541 ccttctgctc catcagccca gacactagcc aattcaactg gtacatagat tgaggcacag
      601 agttctttta agactgcaat ttcagcttct gtatcagata caaattcgtt aatagctaca
      661 actgctggaa taccgaactt acggatattt tcaacgtggc gtttcaagtt agcaaaacct
      721 gcacgaactg cctctacatt ttcttcagtc agagcgtctt tagccacacc accattcatc
      781 ttaagggcac gaagggttgc gacaatgaca actgcatctg gagatgttgg caagtttggt
      841 gtcttgatat caaggaattt ctcagcacca aggtccgcac caaaaccagc ttcagtaaca
      901 gtgtaatcag ccaagtgaag ggctgttgtc gtcgccaaaa cagagttaca gccatgagcg
      961 atattggcaa atggaccacc gtgtacaaag gcaggtgtac cgtaaattgt ctgaaccaag
     1021 tttggcttaa tagcatcctt caaaatcaaa gccaaggcac cctcaacctg caaatcacct
     1081 acagaaacag gcgtacggtc atagcgataa ccaataacga tattcgccaa acgacgtttc
     1141 aagtcctcga tgtccgttgc caagcaaaga attgccatga tttctgaagc aactgtaata
     1201 tcaaaaccat cctcacgtgg aataccgttt agaggaccac caagaccaac agtcacatgg
     1261 cggagcgcac ggtcgttcaa gtccacaacg cgtttccaga ggatacgacg ttgatcaatt
     1321 tccagctcat tcccttggtg caagtggttg tcaatcaagg cagaaagggc attgttggca
     1381 gttgtaatag catgcatatc tccagtaaag tggaggttga tgtcttccat tggcagaact
     1441 tgtgcatacc caccaccagc agcaccaccc ttgatcccca tgactggacc aagagacggt
     1501 tcgcggatag caatcatggt tttcttgcca atcttgttca aggcatccgc aagaccaatg
     1561 gtaagcgtcg actttccttc acctgcaggt gttgggttga tggcagtaac caagatcaat
     1621 ttaccgactg gattgctctc aactgcacga attttatcaa agctgagttt agccttgtac
     1681 tttccgtaca actccaaatc gtcataagaa ataccaagtt tctctacaac atcaacaatt
     1741 ggcttcaact caatactctg tgcgatttca atatctgttt tcattcaaaa ttcctctaac
     1801 ctcttatatg ataattcatt atatcacaaa acaagatttt taacatccta aaactctcta
     1861 aacgttcgta aatatctctg tttttaaggc ttttagagtc ctttcttaaa ttttatatgg
     1921 ctttatagtt tgaaactata gtaaatcttc gtttttacca aaaatttatc actttcattt
     1981 tacttaccgc ttatttttgt gtacaatagt gctatgaaaa ttttagttac atcgggcggt
     2041 accagtgaag ctatcgatag cgtccgctct atcactaacc attctacagg tcacttgggg
     2101 aaaattatca cagagacttt gctttctgca gggcatgaag tttgtttaat tacgacaaaa
     2161 cgagctctga agccagagcc tcatcctaac ctaagtattc gagaaattac caataccaag
     2221 gaccttctaa tagaaatgca agaacgtgtt caggattatc aggtcttgat ccactcaatg
     2281 gctgtttctg actacactcc tgtttatatg acagggcttg aggaagttca ggctagctcc
     2341 aatctaaaag aatttttaag caagcagaat catcaggcca agatttcttc aactgatgag
     2401 gttcaggttt tgttccttaa aaagacaccc aaaatcatat ccctagtcaa ggaatggaat
     2461 cccactattc atctgattgg tttcaaactg ctggttgatg ttaccgaaga tcatctggtt
     2521 gacattgcac gaaaaagtct tatcaagaat caagcagatt taatcatcgc gaatgacctg
     2581 actcaaattt cagcagatca gcaccgagct atatttgttg agaaaaatca gcttcaaaca
     2641 gtccagacta aagaagaaat tgcagaactc ctccttgaaa aaattcaagc ctatcattct
     2701 tagaaaggaa aactatggca aacattctct tggctgtaac gggttcaatc gcctcttata
     2761 agtcggcaga tttagtcagt tctctaaaaa aacaaggcca tcaagtcact gtcttaatga
     2821 ctcaggctgc tacagagttt atccaacctt tgacactaca ggtactctca cagaatcctg
     2881 tccacttgga tgtcatgaag gaaccctatc ctgatcaggt caatcatatc gaacttggaa
     2941 aaaaagcaga tttatttatc gtggtacctg caactgctaa cactattgca aaactagctc
     3001 acggatttgc ggacaacatg gtaaccagta cagctctagc cctaccaagt catattccca
     3061 aactaatagc tcctgctatg aatacaaaaa tgtatgacca tccagtaact cagaataatc
     3121 tgaaaacatt agaaacctac ggctatcagc tgattgctcc taaggaatcc ctactagctt
     3181 gtggagacca cggacgagga gctttagctg acctcacaat tattttagaa agaataaagg
     3241 aaactatcga tgaaaaaacg ctctaatatt gcacccattg ctatcttttt tgctaccatg
     3301 ctcgtgatac actttctgag ctcacttatc tttaaccttt ttccatttcc aatcaaaccg
     3361 accattgttc atattcctgt cattattgcc agcattattt atggtccacg agttggggtt
     3421 acacttggat ttttgatggg attacttagc ttgacggtta acacgattac gattctaccg
     3481 acaagctacc tcttctctcc cttcgtacca aacggaaaca tctactcagc tatcattgcc
     3541 atcgtcccac gtattttgat tggtttaact ccttacttag tctataaact gatgaaaaac
     3601 aagactggtc tgattttagc tggagccctt ggttccttga caaatactat ctttgtcctt
     3661 ggaggaatct tcttcctatt tggaaatgtt tataatggaa atatccaact tcttctggca
     3721 accgttatct caacaaattc aattgctgaa ttggtcattt ctgcaattct aaccctagcc
     3781 attgttccac gactacaaac cttgaaaaaa taaaaacaat ctccgctttc aaacctgaag
     3841 gtggagattt tgctctatac agtttctttt taatgaaatc tttactatag taaaacgaaa
     3901 taagaacagg acaaatcgat caggacagtc aaatcgctaa caatgtttta gaagtagagg
     3961 tggactattc tagtttccat ctactataac ttttaactaa aaaatgatct ataaacccaa
     4021 gtaaattctt gctttattgt ctggtttatt gtaccatact agtgtatatg cagttaaaaa
     4081 ggagattctt gtgaatacac ggaaaaagac acaatttatg acaatgacag cccttttaac
     4141 ggctattgcg attttgattc caattgttat gcctttcaag attgtcattc cacctgcttc
     4201 ctatactttg gggagccaca tcgctatttt tatagccatg ttcttgtcgc ccttgatggc
     4261 agtttttgtc atcctagcct ctagttttgg atttttgatg gctggctatc ccatggttat
     4321 cgtttttcgg gctttttccc atatatcttt tggtgcttta ggagctcttt acctacaaaa
     4381 attccccgat accctagata aaccaaaatc ttcctgggtt ttcaattttg ttttggctgt
     4441 tgttcatgcc cttgctgaag tattagcctg tgtccttttt tacgcaactt cgggtaccaa
     4501 tgtagaaaat atgttctatg ttctatttgt actagttgga tttggcacaa ttatccatag
     4561 tatggtagac tatacattag cactagctgt ctataaagtg cttcgaaaac gccgttaaaa
     4621 ggaaaagcta tgacaaaaga tcgcaaacaa gccctgctcc aactcttgaa agaagctcct
     4681 aaagccctta atggccaaag tttggcggaa cattttcatg tcacacgtca ggtcattgtg
     4741 caggatattg caattttgag agccgatggc gctcctatcc tatccaccaa tcgtggctac
     4801 atctataagc aaattgatac caatccttat gttcacaaac ttttcaaagt gaaacatgaa
     4861 gttgaagaaa tcggtcagga actccttgct attgtcgata atggtgggca tgtccagaat
     4921 accttgattg accatcccgt ttatggagaa attgaaacct tgcttaaact gtcttgtcgc
     4981 agagatgtgc aacattttct agaacaagtc gagcattctg actttagacc tctatctgaa
     5041 ttgacagatg gcatccatta ccacctagtc gaagccgaaa cacaacaaga cctccactat
     5101 atcgaggagg ccttggatca gctaggttat ttagtaaaag actagaagat tttcttttcc
     5161 caatcgtctt ctgtacggcg agggtagaaa aactctgact tgtcgggagc ttccatcatt
     5221 tccatcaatg gtagcatatc ataggccaga tccaagtttg gaatctggtc tttttgcacc
     5281 caagaaactt ctccttcatc tgaagaccga aggctaccag agaactcagt cgccttataa
     5341 caaatgacaa tatagcgccc acctgtatct agtggccaat ttttaatgcc gacaagttga
     5401 ggattttgga tagtcaaccc tgtttcttcg tagatttcac gaatgacaga ctccgcaaaa
     5461 gcctcatcat tttctacatg acctccagga aaggcataac cagaccagcg attgttttca
     5521 ggggcgcgat actgcatcac cacgcgctga gtttcgaggt cttcaatcag acagatattt
     5581 gttaaaattg ttaattggga acgggacata aatttactcg ctttctaatt tattaaattt
     5641 tcagacttac taccagagat attatctttt acttttttaa aattatttag aatagaacac
     5701 cattcgtttt tcctttatag gctttccaag ttcaagtatt ttttcttgtc catcaaaagt
     5761 aaaacccatt ttttgataga aagcaatggc gcgcttgtta tctttcaata cccataagaa
     5821 aatttcagaa aaatgattaa gagcagtcaa agctgctttc attaactttt gtgcgatacc
     5881 ttttccataa tagtctttta aaacatataa agcaataatt tcaccagctt gcatagtctc
     5941 atcacgaaag ttaccataac tgataaaacc aactaccttc agatcaccta tcgcaatcaa
     6001 tgtattttct ggatactttt gactaaagag tcgacatctt tctaatgtca ttgtctcctg
     6061 aaattctgca ggcaaaaggt catcataagc ctctctccac atttgccagt gaacaaggga
     6121 tttaccttct atctctttag gagtttccat tgatttgata ataaccgtca tttattttct
     6181 cccagtcttc tctcaaaata ccatatttaa tactatcaaa atatttacct tgataataac
     6241 gaacttttgg aatatgagct tcttttttca ttcttaattt ttcagcaagt ttcatcatac
     6301 caagattccc tgaccaagtt gtcaaaccca gatgctccaa ctccaagtaa tcctgaaacg
     6361 tcctatctat ccactgcaac atagcaactt tcccaatacc agtgttccag aattttttat
     6421 cataaatacc aattcccaat tccatccatc ttgtttgttt acatacccaa taacgcgaaa
     6481 cagtcccaac tagtttatca tcaacaaaaa taccaaggcg atttgagttg cttaaaatgg
     6541 attctgattt ttgtagttcg aattctttaa aatttgaaaa atactgataa tcatcataat
     6601 agggagcatc atactgtttc caaactggat taagttgtga ataagccatt tcccataaag
     6661 acatcaaatc ttctctcttt aactttctta aataaatcat actattcccc taatccaagg
     6721 ccttgacttc caacatcata tcacggatct gagccgccag ttcaaagtca agcacttcga
     6781 cggcttcttg catttgtttt tctagtttct tgacgagttc tttacgctct tgtttgttga
     6841 ggctattgat gtcgacttcc ttgtcctctt ccttagcaac tgccttggtc acggcaatca
     6901 ggtcacggat ttctttcttg attgtctgtg gtacgatacc atgctcttca ttataggcca
     6961 tctgaatttt ccgacggcgg gcagtttcat cgatagcacg ttgcatagac tgggtaaccg
     7021 tgtctgcata catgatgaca tggccttcac tgttacgagc agcacgtcca atagtctgga
     7081 tgagtccacg ttcgttgcga aggaaacctt ccttgtcagc atcgagaata gctacgaggc
     7141 tcacttcagg aacgtcaatt ccttcacgga gcaggttaat tccgaccaag acatcaaaga
     7201 cacccaagcg caggtcacgg ataatctccg tccgttccaa ggtcttgata tccgagtgca
     7261 tgtacttgac cttgataccc atttccttga agtagtcggt caagtcctct gccattttct
     7321 tggtcaaagt tgtgataaag gtacgctcat ttttttcaac gcgggcattg atttcaccca
     7381 agaggtcatc aatctgtccc atagtcggac ggacttccac ctctggatcc aagagtcccg
     7441 ttggacgaat gatttgctca atcactgtct cggtctgttc attttcatag tcaccaggtg
     7501 tcgctgaaac gtaaacaatc tgatgaacgt gactctcaaa ctcctcccga cggagaggac
     7561 gattgtccaa agcagacggc aaacggaaac cataattaac cagcatttct ttacgcgaac
     7621 ggtctccatt gtacatgccc ttgatttgcc ctatggtcat atgactctcg tcaatcataa
     7681 tcaagaaatc atctgggaag aagtcgagaa gcgtataagg aggctctcct tcgctccgtc
     7741 catccatgtg gcgagaataa ttttcaaccc cattggtata gcccatctca cgcaacattt
     7801 cgatatcata ctctgtccgc tgtttcaaac gctgggcttc aagcagttta ccttcctttt
     7861 caaagacagc taactgctcc tccaactcgg cctgaatctt tgcaatggca acttccatgt
     7921 ggtcgtcatt ggtcacaaag tgtgtcgctg ggaaaatcgc taaatgatcc acttctccca
     7981 acacctgacc tgtcagagcc tcaacttcac gaatacggtc aatttcgtct ccaaaaaatt
     8041 ctactcgaaa ggcatgttca tctcgggaag ctgggaaaat ctctaccaca tccccacgaa
     8101 cacgaaatct tccgcgttgg aaatcaatat cattacgttc aaactgaata tcgaccaagt
     8161 cattcaagag tttatcacga gaaatctcta gaccaggacg gagactaacg acactatcag
     8221 cgtattcctt gggcgaaccc aaaccataga tacaagagac tgaggccacg acaataacat
     8281 cattacgctc caaaagggct gaggtagctg agtggcgaag cttgtcaatc tcgtcattga
     8341 cagaactatc cttctcaata taggtatcgc tagaagggac ataggcctct ggctggtaat
     8401 aatcatagta ggatacgaaa tactcaactg cattttcagg gaaaaattcc ttaaactccc
     8461 catagagctg accagccaga gttttattgt gggcaataac cagagttggt ttattgactt
     8521 tagaaatgac ctgactcata gtataggtct tccctgttcc agtcgccccc atcagaatct
     8581 gagctttttc tcccccctca atgttatcca ccaactgctc gatagcttgg ggttgatctc
     8641 ctgatggttg atattttgat actagtttaa attgattatc tgtaatgtga ttgatcataa
     8701 gaatcctctc tccatgtgct attcctattt tagcatactt atagtatttt cacgaagaaa
     8761 aggacggacc gaagtcacat cctttcattt tctttcttta atttattagc aagatagaca
     8821 atcagcagta gtaataccag acttgtcata atggaacctt ctatgccaaa agaaccacct
     8881 gatagccaat cttgattgcc ttgtggtaaa aaggtcatca gagacgttcc tgacggttga
     8941 ccactaacta aaatcccaaa gagattaccc tgagcaaaat tccaagcacc atgaatacct
     9001 gcaacacccc aaactgtatc agttttgaga agataaagag ccatggcaac tccgaataaa
     9061 aagagattta ctagagatag aggggtgagg ctagaattgc ccatatgaag cagggtaaag
     9121 aacaggctag atataagaat agctagtttt agattggttc ttgaggccaa ttgaggaagt
     9181 agccaagcac gggccaccac ttcttctgct gtcccctgta aaatccaaaa tgggatagta
     9241 aagacgacaa aggcaagaga ataaggattc aagtgaatgg attccaaacg atattgacct
     9301 aagaccacta aacctaacaa ggtcagaaga aaaagtgcca agcctagact aaatcctttc
     9361 agaagattgc tgaggaaatt ctctctataa aatcccaagg ttcgaatagg tcttttctca
     9421 acttttcttg tccatctgaa cacagtgtta agaataaaac caaaggccag taatcctaaa
     9481 attaaacgaa agtcacttgt tttatccgtc aagctctgtt gcaaggtctg caagtagctt
     9541 gcaagatttt gaccagcctc tccaaaattt ctagcaagtc cgatgagagc taacaagcca
     9601 ataccataca aagtaaaagc cacaaactct cctatgaaga caaaaacaaa ggctaacaag
     9661 gggcttgtgt aaatgtttaa actcattttt gaagcttgga agtctttaaa tattcttttg
     9721 ttcttcatat ccccgtcctc ttttcttc
//
LOCUS       DAVLVF010000072         9741 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVLVF010000072 DAVLVF010000000
VERSION     DAVLVF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9741
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.072"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            117..3218
                     /locus_tag="VYN66_001606"
     CDS             117..3218
                     /locus_tag="VYN66_001606"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV1495602.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVSYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDSALEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNYSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKSIYIGLKSIKGLSKDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3301..4308
                     /gene="pfkA"
                     /locus_tag="VYN66_001607"
     CDS             3301..4308
                     /gene="pfkA"
                     /locus_tag="VYN66_001607"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV1495603.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4367..5872
                     /gene="pyk"
                     /locus_tag="VYN66_001608"
     CDS             4367..5872
                     /gene="pyk"
                     /locus_tag="VYN66_001608"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV1495604.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6607..6832)
                     /locus_tag="VYN66_001609"
                     /pseudo
     CDS             complement(6607..6832)
                     /locus_tag="VYN66_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7680..8552
                     /locus_tag="VYN66_001610"
     CDS             7680..8552
                     /locus_tag="VYN66_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV1495605.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8698..9042)
                     /locus_tag="VYN66_001611"
                     /pseudo
     CDS             complement(8698..9042)
                     /locus_tag="VYN66_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9185..9497
                     /locus_tag="VYN66_001612"
                     /pseudo
     CDS             9185..9497
                     /locus_tag="VYN66_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9501..9741
                     /locus_tag="VYN66_001613"
                     /pseudo
     CDS             9501..9741
                     /locus_tag="VYN66_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT         3018 a         1688 c         2071 g         2964 t
ORIGIN      
        1 caaaaataca aatgaataga aaataaatta gtaagctgat gaaatttttc tcaagagaag
       61 ccatttatag gtgaaaatgg tataatatag tgagaaggat agaggagaag tgtaaattga
      121 tcgcacaact agatacaaaa acagtctata gttttatgga aagcgtcatt tcgatcgaaa
      181 agtatgtgag agcagctaaa gaatacggct acactcattt ggctatgatg gatattgaca
      241 atctttatgg cgctttcgac tttctagaga ttacaaaaaa atacggcatt catcctttgc
      301 tagggcttga aatgacagtg tttgtagatg atcaggaagt gaatttgcgc tttttagctc
      361 tatctagtgt gggctatcag cagttgatga agctttcgac agccaagatg cagggggaga
      421 aaacttggtc agtcctgtcc cagtacctgg aggatatcgc ggtcattgtg tcttattttg
      481 atagagttga gtcgttagaa ctaggctgtg attactatat aggggtttat ccagaaacac
      541 tagcaagcga atttcatcat cctatcttac ctctttatcg ggtcaacgct tttgaaagca
      601 gggatagaga agttcttcaa gttttaacag cgattaaaga aaatctaccg ctcagagaag
      661 ttcccttgcg ttcgagacaa gatgtcttta tatcagcaag ttctttagag aaactattcc
      721 aagagcgttt tccgcaagct ttggacaatt tagaaaagct tatttcaggc atttcttacg
      781 acttggatac tagtctgaaa ctgcctcgtt ttaatccagc tagaccagca gtagaggagt
      841 tgagagagcg tgctgaactg gggcttgttc agaaggggtt gactagtaaa gaatatcaag
      901 atagactaga ccaagaattg tctgttattc atgatatggg ctttgatgat tatttcttgg
      961 ttgtttggga tttgttgcgt tttggacgat cgaatggcta ttatatggga atgggaaggg
     1021 gttctgcagt aggcagtttg gtttcttatg ccttagacat cacggggatt gacccagtag
     1081 agaaaaatct gatttttgaa cgctttctta atcgtgaacg ctataccatg cctgatattg
     1141 atattgatat cccagatatt tatcgtccag attttatcag atatgttggt aataaatatg
     1201 gtagtaaaca tgcggcacaa atcgttactt tttcaacctt tggagccaag caagctcttc
     1261 gagatgtctt gaaacgcttt ggtgtgccag agtatgaatt atctgcaatt actaagaaaa
     1321 tcagttttcg tgacaatctt aagtcggcct atgagggcaa tctccagttt cgtcagcaaa
     1381 tcaatagtaa gttagaatac caaaaagctt ttgagattgc ttgcaagata gagggctatc
     1441 caaggcaaac ctctgtccat gcggctggtg ttgtaattag tgaccaagat ttaaccaact
     1501 acattcctct aaagtatggt gatgaaattc cactgactca gtatgatgct catggagttg
     1561 aggctagcgg acttttgaag atggactttc tgggactacg aaatttgacc tttgtccaga
     1621 agatgcaaga gttgcttgct gaaacagaag gtattcatct gaaaattgaa gaaatcgatt
     1681 tagaagacaa agaaacgtta gctttatttg cctctggtaa tacaaaaggt atctttcaat
     1741 ttgagcaacc aggtgccatt cgtctgctta agcgtgtgca accagtctgt tttgaagatg
     1801 tcgtcgcgac tacttctcta aatcgaccag gtgcaagtga ctacatcaat aattttgtgg
     1861 caaggaagca tgggcaggaa gaagtgactg ttctagattc agctctggag gatattttgg
     1921 ctccaactta tggcattatg ctctatcagg agcaggttat gcaggttgct cagcgatttg
     1981 ctggatttag tcttgggaag gccgatattt tgcgtcgagc tatgggtaaa aaggatgcct
     2041 ctgctatgca tgagatgagg gcttccttta ttcaaggctc catagaagca ggccatactg
     2101 cggaaaaagc agagcaggtt tttgatgtta tggagaagtt tgcaggttat ggatttaata
     2161 gatctcatgc ctacgcctac tcagccttgg ccttccagtt ggcttatttc aaaacgcatt
     2221 atccagccat tttttatcag gtcatgttaa attattccaa cagtgattac ttaatagatg
     2281 cacttgaagc aggttttgaa gtagcctctc tatccatcaa tactattccc tatcacgata
     2341 aaattgccaa caagtctatc tatataggtt tgaaatcgat taaggggctc agcaaggact
     2401 tggcgctttg gattattgaa catagacctt attctaacat tgaagatttt atagctaaat
     2461 tacctgagaa ttatctgaaa cttcctctgc tagaaccttt ggtaaaagtt ggtcttttcg
     2521 attcatttga aaaaaatcgt caaaaagtat ttaataactt agctaatcta tttgaatttg
     2581 tgaaagagtt gggaagtttg tttggagatg ctatttatag ttggcaggaa tcggaagatt
     2641 ggacggaaca agaaaaattt tatatggaac aagagctttt agggataggt gtcagcaaac
     2701 atccactaca agctattgca agtaaggcta tttacccgat taccccaatc ggaaatttgt
     2761 cagaaaatag ctatgctatt attttggttg aagttcagaa aataaaagtg attcgtacca
     2821 aaaagggtga aaatatggcc ttcttacagg cagatgatag taagaaaaaa ttggatgtca
     2881 ctctcttttc agacttatat cgtcaggttg gacaggaaat aaaagaggga gccttctact
     2941 atgtaaaagg aaaaatacaa tcacgtgatg gccgtctgca aatgattgca caagaaataa
     3001 gagaagcagt tgctgaacgc ttttggatac aggtgaaaaa tcatgaatcg gatcaagaaa
     3061 tttcacgcat tttagaacaa tttaaaggcc caatcccagt catcatccgg tatgaagagg
     3121 aacagaaaac catcgtttct ccccatcatt ttgtagctaa atccaatgaa ttagaggaga
     3181 aattgaatga aatcgttatg aaaacgattt atcgctaaaa atacggaaaa tagaagaatt
     3241 ttaaaatcaa atgtgatata atcaagaaga atgttaaaag aaaaaggagc ataaaccaat
     3301 atgaaacgta ttgctgtttt gactagtggc ggagacgcac ctggtatgaa cgctgccatc
     3361 cgtgcagttg ttcgtcaagc aatttcagaa ggaatggaag tgtttggtat ctatgacgga
     3421 tatgctggta tggttgccgg tgaaattcat cccctagatg cagcttcagt aggggacatc
     3481 atttctcgtg gtggtacttt tcttcactca gctcgttatc cagagttcgc tcaacttgaa
     3541 gggcaactta aagggattga gcaattgaaa aaacacggaa ttgaaggtgt agttgttatc
     3601 ggtggtgacg gatcttacca cggcgctatg cgtttgactg aacatggctt cccagctatt
     3661 ggtcttccag gtacaatcga taacgatatc gttggtactg actttacaat cggttttgac
     3721 acagcggtta ctactgccat ggacgctatc gataagattc gtgatacatc atcaagtcac
     3781 cgtcgtactt ttgtaatcga agttatggga cgtaacgctg gtgatatcgc tctttgggct
     3841 ggtattgcaa ctggtgctga tgaaatcatc atccctgaag caggcttcaa gatggaagat
     3901 atcgtagcaa gcatcaaagc tggttatgaa tgtggtaaaa aacacaatat catcgtctta
     3961 gctgaaggtg tgatgtcagc ggctgaattt ggtcaaaaac ttaaagaagc tggagataca
     4021 agcgaccttc gtgtaacaga acttggacat attcaacgtg gtggttctcc aactgcgcgt
     4081 gaccgtgttt tggcgtcacg tatgggtgca catgctgtta aacttcttaa agaaggtatc
     4141 ggtggtgttg cggttggtat tcgtaacgaa aaaatggttg aaaatccaat tcttggtact
     4201 gcagaagaag gggcattgtt tagccttact gcagaaggta agattgtggt taacaacccg
     4261 cacaaagccg atattgagct atctagcttg aataagagct tgtcataatt tacaatttag
     4321 ttaataagac caaaaaggtc atatatataa aggagtcaca aaaatcatga acaaacgtgt
     4381 aaagatcgtt gcaactttgg gtcctgcggt agaaatccgt ggtggtaaaa aattcggtga
     4441 ggacggatac tggggtgaaa aacttgatgt tgaagcttca gctaaaaaca ttgctaaatt
     4501 gattgaagct ggtgctaaca cattccgatt caacttctca cacggcgacc accaagaaca
     4561 aggtgagcgt atggcaactg ttaaacttgc ggaaaaaatt gcaggtaaaa aagttggttt
     4621 ccttcttgat acaaaaggac ctgaaatccg tacagaattg ttcgaaggtg aagctaaaga
     4681 atattcatac aaaactggtg aaaaaattcg tgttgcaact aaacaaggaa tcaaatcaac
     4741 tcgtgaagtg attgcgttga acgttgctgg tgctcttgat atctatgatg atgttgaagt
     4801 tggtcgtcaa gttttggttg acgatggtaa acttggtctt cgtgtggttg ctaaagatga
     4861 tgcaactcgt gaatttgaag ttgaagttga aaacgatggt atcatcgcta aacaaaaagg
     4921 tgtgaacatc cctaacacta aaattccttt cccagctctt gctgaacgcg ataacgacga
     4981 tatccgtttc ggtcttgaac aaggtatcaa cttcatcgca atttcattcg tacgtactgc
     5041 aaaagatgtg aacgaagttc gtgcaatctg tgaagaaact ggaaacggac atgttcaatt
     5101 gttcgctaaa atcgaaaacc aacaaggtat cgataactta gatgaaatca tcgaagcagc
     5161 tgatggtatt atgattgctc gtggtgatat gggtatcgaa gtaccgttcg aaatggttcc
     5221 agtttatcaa aaaatgatta tcaagaaagt caatgctgca ggtaaagttg ttatcactgc
     5281 aacaaacatg cttgaaacaa tgactgaaaa accacgtgca actcgttcag aagtatcaga
     5341 cgtattcaac gctgttatcg acggaactga cgctacaatg ttgtcaggcg agtctgcaaa
     5401 cggtaaatac ccactcgagt cagtaactac aatggctaca atcgacaaga acgctcaagc
     5461 tcttcttaat gaatacggac gtcttgattc agattcattt gagcgtaact ctaagacaga
     5521 agtaatggct tctgctgtta aagatgctac tagctcaatg gatatcaaat tggttgtaac
     5581 tcttactaag acaggtcata ctgcacgttt gatttctaaa taccgtccaa atgctgacat
     5641 cttagcattg acatttgacg aattgacaga acgtggcttg atgttgaact ggggtgttat
     5701 cccaatgttg acagatgctc catcttcaac tgacgatatg ttcgaaatcg ctgaacgtaa
     5761 agcggtagaa gcaggtctcg ttgagtcagg cgatgatatc gttatcgttg ctggtgtgcc
     5821 agtaggagaa gctgttcgca caaacacaat gcgtatccgc acagtacgtt aagaaaaata
     5881 taaaaaccta tcatatccag ctttctagcc tgtatgatgg gctctttttt tgtccagggg
     5941 gcaaaaaggg ggcaaaactt tcataatgga ttgatactct tcgaaaatca aattcaaacc
     6001 acgtcagcgt cgccttaccg tatatatgtt actgacttcg tcagttctat ctacaacctc
     6061 aaagcagtgc tttgagcaac ctgcggctag cttcctagtt tgctctttga ttttcattga
     6121 gtatgaaata agatatgcat aaattgatta gaaagtcaaa tgaatttcta caaatgtttt
     6181 agcaatcgca atctacttgt ctagattcga tctgatatat tttcgattta atgatatggt
     6241 atttaaaacc tccaaagtag cttgctccat tcttttacct acgtgagtct agatgttatt
     6301 tactgtttta gcgtttttgt gttctactct aaccattata gcattcttct cagctagtgt
     6361 actaaggagt gtgtgcctga aaatatggga actaaggggc tggtttatcg gtttctctag
     6421 tttagtattt gccttttgca aagtgatctt aaatgccttt ctctaaattt acatatcact
     6481 attgtttaac aaaatctaat ctattttagg tcacttattc tttttttgaa atgtagaatg
     6541 aactttttca aagtttttcg aatcttttaa aatctgtttg ttttatatcg ccattctccc
     6601 ccttttttaa ttctccctat atagcctgac aacttttccg atggtacgaa tatggttgct
     6661 ttcgtctagg tggatgtcgg agtattcggg attgagtttt tttgaggtag ccttggcgga
     6721 gtttcttgac atagttagtg ccgtctactt ggaagatgcc gatggtatta tagtcaatct
     6781 gtggggtatt cttgataaat aggtagtcgc tgtttcttat ctttggctcc atggacttgc
     6841 tgacgacata agcgattggg tcgtagtcgt ctgggataat ggaaactcca taactaaatc
     6901 gtcgtcctgc atcaagcggc tacctgcaga gataaactac ctaacacgag agtaagtagt
     6961 ctgtctgtag tcgtccagtc tgatgatttt tacgatactt cgtttttctg atcatacagt
     7021 tgcctctcgg cataggtcag aactttacct tgtctgggtg gtgcccgttg gtcgtagata
     7081 gattggatat cgctaggaga atccttttga actggaggaa agagggcatc aatcaagcta
     7141 ctgaatactt taactaagtc aaatatagta tttttcttag tagacctaac ccttttttca
     7201 taatttctaa tggtgttttt acttatacct atcttagtac ccaattctta ttgagtccaa
     7261 ccattactag tctatattgt tttatagttg attgagtttg gaatagtacg ctgtagctgc
     7321 taaaacattt ctaggaatta atttgccttt cctaatagag ttgttcatat cttatttcaa
     7381 tctactatat ttttcacctc taacaatcgc aatctcttct ttatccatga acgaaaacgc
     7441 tttctatctt tgtaagtaaa gcatcacacg aaatccacga aaatgaaaac ctttgttgtg
     7501 ttttcgtaaa aaatttgttg acagagcacg aaacgcctac tataattaaa tcaagtttaa
     7561 ggaaataaca aaaaaacaaa tcggagggaa agacgatgaa cacattaaat gagaaagtaa
     7621 tcaatatctg taaagcagta gttaaagaaa ctttaatcca agacatttag gagaaagaaa
     7681 tgaagaaatc aagaaaatta gccacattag gaatctgttc agccttgttt ttaggcttgg
     7741 ctgcttgtca acaacaacat gctacttctg aggggacgaa tcaaaggcaa agcagttcag
     7801 cgaaagttcc atggaaagct tcatacacca acctaaacaa ccaggtaagt acagaagagg
     7861 tcaaatctct cttatcagct cacttggatc caaatagtgt tgatgcattt tttaatctcg
     7921 ttaatgacta taataccatt gtcggctcaa ctggcttatc aggagatttc acttccttta
     7981 ctcacaccga atacgatgtt gagaaaatca gtcatctctg gaatcaaaag aagggcgatt
     8041 tcgttgggac caactgccgt atcaatagtt attgtctttt gaaaaattca gtcaccattc
     8101 caaagcttga aaagaatgac cagttgcttt tcctagataa tgatgcgatt gataaaggaa
     8161 aggtctttga ttcacaagat aaggaagagt ttgatattct attttcgaga gttccaactg
     8221 aggcaactac agatgtcaag gttcacgctg aaaagatgga aacattcttc tcacaatttc
     8281 aattcaatga aaaagctcga atgctgtctg tagtcttgca cgacaatctg gatggcgagt
     8341 atctgtttgt aggccacgtt ggggtcttag tacctgctga tgacggtttc ttatttgtag
     8401 agaaattgac tttcgaagag ccctaccaag cgattaaatt tgctagtaag gaagattgct
     8461 acaagtattt aggcaccaag tatgcggatt atacaggcga gggactggct aagcctttta
     8521 tcatggataa tgataagtgg gttaaacttt aatagtgtta tgatatgaca gtaatcttag
     8581 gatttttatt tctatttttg atgagttggt gggttatttc aatcgttaat agccagaaac
     8641 cacgcaaaaa tgaaacattt attggctata tccaaagata cgatattgat gggaatggct
     8701 atcaatcatt accgtgtcct cagaactgag aggagttctt gaaatcgtaa aactactttg
     8761 aacaagagtt acttcaaccc attggctccg acggattaag ttgctttcgt gaacaccaaa
     8821 atcagccgca atttcttcat aagtgcggta ttctcgcaca tattgaagag tggccataag
     8881 aaggtcttct aggcttaatt taggttttcg tccccctttt gtgtgtttaa gttgataagc
     8941 agtttttaat acagctaaca tctcttcaaa agtggtgcgc tgaacaccaa caagacattt
     9001 aaatcgtgca tcagttagtt gcttacttgc ttcataattc atagaactac tataccatat
     9061 tttgtttcgc aggaagtcta atgctaaaat cttatcagtg agaagatttc ttaaagttta
     9121 ttctttgtag tttcatctgt attagtacat aatttaactt agagaaaata ggaggattaa
     9181 tcgaatggaa ttaaaagatt ttacagaaaa agaacaggaa atgattaaga aaaggcttac
     9241 aatgtctaat attagtgaca aagaaactac tgagaagatt ctggcgctag taccacaaga
     9301 cttgattaag cgaatcccgt tttttgtcag aaaacatgct acaacacgta cgattaaacg
     9361 catttcaatt gaacaccctg aactctacgc tgtagctcaa acaagtggtg agattccaga
     9421 aaaaaaaacg cgaagaattg cgtcagatta tcacaactat ctttgaacaa aagatgaata
     9481 agcatagtat taagtagaga atgaaaaaat attttattgg cggtttggga agcaatgcct
     9541 atcatagcaa ggattttctt taagaactag attcgcaggt ctattttcta aatccatatg
     9601 aaaagcatct tcgagatgaa acagaattga aatcatggtt taaaaatgag attgtagagg
     9661 aagaatctat ctgtctgata ggtcattcta ttggaggcga tttagctcgt tatttcgcat
     9721 cggaatttga agaagtaaag a
//
LOCUS       DAVLVF010000073         9397 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVLVF010000073 DAVLVF010000000
VERSION     DAVLVF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.073"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(68..1048)
                     /gene="dusB"
                     /locus_tag="VYN66_001614"
     CDS             complement(68..1048)
                     /gene="dusB"
                     /locus_tag="VYN66_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV1495606.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1035..1907)
                     /gene="hslO"
                     /locus_tag="VYN66_001615"
     CDS             complement(1035..1907)
                     /gene="hslO"
                     /locus_tag="VYN66_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV1495607.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGVEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2072..3499
                     /locus_tag="VYN66_001616"
     CDS             2072..3499
                     /locus_tag="VYN66_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1495608.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            3658..5166
                     /gene="glpK"
                     /locus_tag="VYN66_001617"
     CDS             3658..5166
                     /gene="glpK"
                     /locus_tag="VYN66_001617"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV1495609.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNFVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5208..7034
                     /gene="glpO"
                     /locus_tag="VYN66_001618"
     CDS             5208..7034
                     /gene="glpO"
                     /locus_tag="VYN66_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV1495610.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKKEVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7104..7808
                     /locus_tag="VYN66_001619"
     CDS             7104..7808
                     /locus_tag="VYN66_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1495611.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8105..8308
                     /locus_tag="VYN66_001620"
     CDS             8105..8308
                     /locus_tag="VYN66_001620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495612.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(8395..9003)
                     /locus_tag="VYN66_001621"
     CDS             complement(8395..9003)
                     /locus_tag="VYN66_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495613.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT         2696 a         1871 c         2146 g         2684 t
ORIGIN      
        1 tttttttgat gaggaaagaa tggcggcact caagagactc tttaagagag ttacgggttt
       61 taaactatta agccttctcc aattgcaaga gagcttcaat ctctgccagg gtgctagctt
      121 gcgaaatggc tccacggagt ttggcagcgc cagatgttcc acggaggtag tgaggagcga
      181 ggccacggaa ttcacgaact gcgacgtttt ctcctttgag gttaatcaat cgtttcaagt
      241 gttcgtaggc gatcttcatc ttgtcttcaa aggtcaaatc aggtaggatt tctcctgttt
      301 caaagtaatg gttgatttgg ttgaagaggt aaggatttcc catggcagct cggccaatca
      361 tgactgcgtc agcaccaact tcttcgatgc gttgcttggc ttcttggaca gtacggatat
      421 caccgttggc gatgaatgga atcttggtta gagcttgggc aaccttgtac agggtctcaa
      481 ggtctgcgtg gccagtatac atttgttcac gggtacggcc atgcatggcg agggcagaaa
      541 cacctgcagc ctcagcagcg agggcatttt ctactgccag agatgggtcc gcccagccgg
      601 tacgcatttt gacagtaagt gggatatcaa ggacagactg gaccttgttg atgatagagt
      661 agatcttgtc aggatccttg agccacatag ctccagcttc gttcttcacg attttgttga
      721 cagggcagcc catgttgata tcgacgatat cggtcttggt gttttcttgg atgaattctg
      781 ctgcgcgtgc taggctgtct tcatcgctac caaaaagttg gatagagaca gggttttcgc
      841 cctcatcgat atgaagcatg tgcagggttt tttcgttgtt gtattggatt cccttgtcag
      901 agaccatttc cattacaacg agtccagctc cgagctcttt tgcgatggta cgaaaggctg
      961 agttggtcac gccagccata ggcgctaaaa cggtacgatt gggaatctca acattgccaa
     1021 tcataaaagg tgtattaaga tttgtcacga atgagttcct ccaggtcctt ttcatcaaag
     1081 ttgtaagtag tttggcagaa ttgacaagtg atttctaccc cgtggtcttc ctctttcatt
     1141 tcctgtaagt ctgagcttgg aaggctggca agagcgttca taaagcgttc atggctacag
     1201 tcacattgga aacggatttc ttcttcagaa agacgcttgt aggcttcgtc cccgtagata
     1261 gccttgagga gggcttcgat atggtcgtcg ctttcgagaa gagtagagat agctggcatt
     1321 tcttggatgc gtttttcaaa gcgagcaatc tcttctttct tggctcctgg caagacttga
     1381 actaggaaac cacctgcaac cttgaccttg tcttcctcgt ccaaaaggac attgaggccg
     1441 accgctgaag gcgtttgttg gctttcagta aggtaaaagg caaggtcttc accgatttct
     1501 ccagagatga ggggagttat agagttgtaa ggatttccag caccgtagtc tgtgataacg
     1561 aggaattgac catttccaac aaaaggtccg actaggactt caccagtcgc agtctttttg
     1621 atgtcaacac caggattttg aacatagcct ttgacgttcc ccttggtatc agcgacggtg
     1681 ataatagcac ctagagagct agagcccaac accttaactg taagtttggt atttcctttt
     1741 tcattggctg cgagaatctg gctggcgata agagttcgac caagcgctac agttgagcta
     1801 gcttgggttt gatgtttttc ttgagcagtg cggacggttt ccgtgctatc aaggacaaaa
     1861 gcacgaaagg ctccgctttc tgatatagtt ttaataattt tatccatagc tactatttta
     1921 gcataaaaat gcccaaaggg ggagccgtgt gtttactgat tttcaggata atggaccagg
     1981 aaatcagcat gaaaataaaa agagaaacag attattttag catttgtaag atttatgcta
     2041 tgcttaaggt agaaaatgaa aggggtaaca aatgtattta ggagatttga tggagaaagc
     2101 cgagtgtggt caattttcaa tcctttcctt tctattacaa gagtctcaga cgaccgtcaa
     2161 ggctgtaatg gaagaaacag gattttcaaa agcaacccta accaaatatg tcaccctgct
     2221 caatgacaag gctttggata gtggcttaga gctggctatt cactcagaag atgaaaatct
     2281 gcgtctgtct atcggtgcag ctaccaaggg gagagatatt cggagcttgt ttttggagag
     2341 tgctgttaaa taccagattt tggtttatct tctctaccac caacagtttt tagcccatca
     2401 gctggctcaa gaattggtga ttagcgaggc tacgcttggt cgtcacttgg ctggtttaaa
     2461 tcagattttg tcagaatttg atttatccat ccaaaatgga cgttggcgag gtccagagca
     2521 tcagattcgc tatttctatt tctgtctttt ccgaaaggtc tggtcgagtc aggaatggga
     2581 aggtcacatg cagaaaccag agagaaaaca ggagattgcc aatttagagg aaatctgcgg
     2641 tgcaagtttg tctgtggggc agaaattgga cttggttctc tgggctcaca tcagtcaaca
     2701 acgtcttcgg gtcaatgctt gtcagtttca agtcatagaa gagaaaatgc gagggtattt
     2761 tgacaatatt ttctaccttc gtttgctgag aaaggttccg tccttttttg ctgggcaaca
     2821 tattccacta ggagttgagg atggtgagat gatgatattc ttctcttttc tcctatctca
     2881 tcgcattctt cctcttcata ctatggagta tattcttggt tttggagggc agttggcaga
     2941 tttactgacg caattgattc aagaaatgaa gaaggaggaa ctattggggg attatacaga
     3001 ggaccatgtc acctatgaac tcagtcagct ttgtgctcaa gtctatctct ataagggcta
     3061 tattttacag gatcgctaca agtaccagtt agagaatcgt catccatatt tactgatgga
     3121 acatgatttt aaagagacag cagaggagat ttttcatgct ctacctgctt ttcaacaggg
     3181 gacagattta gataagaaga ttctctggga atggctccag ttaatcgaat atatggctga
     3241 aaacggtggc cagcatatgc ggattggtct ggatttgaca tctggttttc ttgtcttttc
     3301 aaggatggca gccattttga aacggtattt ggaatacaat cgttttatta ccattgaagc
     3361 ctatgaccct agtcggcatt atgatttgct ggttaccaat aacccgattc ataagaagga
     3421 acagacacca gtctattatt taaaaaatga cttggatatg gaggatttgg tagcgattcg
     3481 ccagttatta ttcacttaaa aggcttggtt aatccaggtc ttttttgtga aattcacaca
     3541 atctcctcac atttttttaa aaattaaaaa aagttgataa acaagaaagc gctttatttt
     3601 gtatactagt aagtgtaaag aggaaacacc tcaagatctt tatcaggagg acagtacatg
     3661 tcacaagaaa aatacatcat ggccattgac cagggaacta caagttctcg tgccatcatt
     3721 ttcaacaaaa aaggggaaaa ggttagctcg agtcaaaaag agtttaccca gattttccct
     3781 caggcaggtt gggttgagca caatgccaat gaaatttgga actttgttca gtcagttatt
     3841 gcgggtgctt tcatcgaaag tggtgtcaag ccaaatcaaa tcgaggcaat cgggattacc
     3901 aaccaacgtg aaacaacggt tgtctgggat aagaaaacag gacttcctat ctacaatgct
     3961 atcgtttggc agtcacgcca gacagcacct ttggctgagc aactaaaaag ccaaggttat
     4021 gtggaaaaat tccatgaaaa gactggtttg attattgatg cttacttctc tgctaccaag
     4081 gttcgttgga ttttggatca tgtagaaggt gctcaagagc gagcagaaaa aggggaattg
     4141 ctctttggta ctatcgatac ttggttggtt tggaaattga ctgacggtgc ggctcacgtg
     4201 actgactact caaatgcagc tcgtaccatg ctttataaca ttaaagaact caaatgggat
     4261 gatgagattt tggaaatcct taacattccg aaggctatac ttccagaagt tcgttctaac
     4321 tccgaaatct acggcaagac agctccattc catttctacg gtggagaggt gccaatctca
     4381 ggtatggctg gggaccaaca agcagccctc tttggacagt tggcttttga gccaggtatg
     4441 gttaagaata cttatggaac aggctctttc atcatcatga atactgggga agagatgcag
     4501 ttgtctgaaa acaacctctt gacaaccatt ggttacggaa tcaacggtaa ggtttattat
     4561 gccttggaag gttctatctt catcgcagga agtgctattc agtggcttcg tgacggtctt
     4621 cgcatggttg aaaattcacc agaatctgaa aaatacgctc gtgattctca caacaacgat
     4681 gaagtttatg tcgttccagc ctttacaggt ctaggcgctc catactggaa ccaaaatgct
     4741 cgtggttccg tctttggttt gactcgtgga acaagcaaag aagactttat caaggcgact
     4801 ttgcaatcta ttgcttatca agtgcgtgat atcatcgaca ccatgcaagt ggatactcag
     4861 accgccattc aagtactgaa ggtggatggt ggtgcagcca tgaacaactt cctcatgcag
     4921 ttccaggcgg atattttagg cattgacatt gcacgtgcta aaaacttgga aacaacagct
     4981 ctaggagcgg ccttcctagc aggtttgtca gtagggtact ggaaagactt ggacgagttg
     5041 aaactcttga acgagacagg agaactcttt gagccatcta tgaacgaatc tcgcaaggaa
     5101 caactctaca agggctggaa gaaggctgtg aaagcaactc aagtctttgc ggaagtagac
     5161 gactaatact ggcagaataa agcgatttat ttagaaagtg tgtaaatatg gaattttcaa
     5221 aaaaaacacg tgaattgtca attaaaaaaa tgcaggaacg caccctggac ctcttgatta
     5281 tcggtggagg aatcacagga gctggtgtag ccttgcaggc ggcagctagc ggtcttgaga
     5341 ctggtttgat tgaaatgcaa gactttgcag aaggaacatc tagtcgttca acaaaattgg
     5401 ttcacggagg tcttcgctac ctcaaacaat tcgacgtaga agtggtctca gatacggttt
     5461 ctgaacgtgc agtggttcaa caaatcgctc cacacattcc aaaaccagat ccaatgctct
     5521 taccagttta cgatgaagat ggagcaacct ttagcctctt ccgtcttaaa gtagccatgg
     5581 acttgtacga cctcttggca ggtgttagca acacaccaac tgcgaacaag gttttgagca
     5641 agaaagaagt cttggaacgc cagccaaact tgaagaagga aggcttggta ggaggtggag
     5701 tgtatcttga cttccgtaac aacgatgcgc gtctcgtgat tgaaaacatc aaacgtgcca
     5761 accaagacgg tgccctcatt gccaaccacg tgaaggcaga aggcttcctc tttgacgaaa
     5821 gtggcaagat tacaggtgtt gtagctcgtg atctcttgac agatcaagta tttgaaatca
     5881 aggcccgtct ggttattaat acaacaggtc cttggagtga taaagtacgt aatttgtcta
     5941 ataagggaac gcaattctca caaatgcgcc caactaaggg agttcacttg gtagtggatt
     6001 caagcaaaat caaggtttca cagccagttt acttcgacac aggtttgggt gacggtcgta
     6061 tggtctttgt tctcccacgt gaaaacaaga cttactttgg tacaactgat acagactaca
     6121 caggtgattt ggagcatcca aaagtaactc aagaagatgt agattatcta cttggcattg
     6181 tcaacaaccg ctttccagaa tccaacatca ccattgatga tatcgaaagc agctgggcag
     6241 gtcttcgtcc attgattgca gggaacagtg cctctgacta taatggtgga aataacggta
     6301 ccatcagtga tgaaagcttt gacaacttga ttgcgactgt tgaatcttat ctctccaaag
     6361 aaaaaacacg tgaagatgtt gagtctgctg tcagcaagct tgaaagtagc acatctgaga
     6421 aatatttgga tccatctgca gtttctcgtg ggtctagctt ggaccgtgat gacaatggtc
     6481 ttttgactct tgctggcggt aaaatcacag actaccgtaa gatggctgaa ggagctatgg
     6541 agcgcgtggt tgacatcctc aaagcagaat ttgaccgtag ctttaaattg atcaattcta
     6601 aaacttaccc tgtttcaggt ggagaattga acccagcaaa tgtggattca gaaatcgaag
     6661 cctttgcgca acttggagta tcacgtggtt tggatagcaa ggaagctcac tatctggcaa
     6721 atctttacgg ttcaaatgca ccgaaggtct ttgcacttgc tcacagcttg gaacaagcgc
     6781 caggactcag cttggcagat accttgtccc ttcactatgc aatgcgcaac gagttggctc
     6841 ttagcccagt tgacttcctt cttcgtcgta ccaaccatat gctctttatg cgtgatagct
     6901 tggatagcat cgttgagcca gttttggatg aaatgggacg attctatgac tggacagaag
     6961 aagaaaaagc aacttaccgt gctgatgtcg aagcagctct cgctaacaac gatttagcag
     7021 aattaaaaaa ttaagaaaaa ataaaagagg tggagggcag cattccttgt cgcccgtccc
     7081 ttctttttaa tggagacaga aagatgatga atgaattatt tggagaattt ctagggactt
     7141 taatcctgat tcttctagga aatggtgttg ttgcaggtgt ggttcttcct aaaaccaaga
     7201 gcaatagctc aggttggatt gtgattacta tgggttgggg gattgcagtt gcggttgcag
     7261 tctttgtatc tggcaagctc agtccagctc atttaaatcc agctgtgaca gttggtgtag
     7321 ctttaaaagg tgacttgcct tgggcttccg ttttgcctta tatcttagcc cagttcgcag
     7381 gggccatgtt gggtcagatt ttggtttggt tgcaattcaa acctcactat gaggcagaag
     7441 aaaatgcagg caatatcctg gcaaccttca gtactggacc agccatcaag gatactgtat
     7501 caaacttgat tagcgaaatc cttggaacct ttgttttggt gttgacaatc tttgctttgg
     7561 gtctttacga ttttcaggca ggtatcggaa cctttgcagt gggaactttg attgtcggta
     7621 tcggtctatc actaggtggg acaacaggtt atgccttgaa cccagctcgt gaccttggac
     7681 ctcgtatcat gcacagcatc ttgccaattc caaacaaggg agacggagac tggtcttacg
     7741 cttggattcc tgttgtaggc cctgttatcg gagcagcctt ggcagtgctt gtattctcac
     7801 ttttctagtt tatactcttc gaaaatcaaa ttcaaaccac gtcagcgtcg ccttaccgta
     7861 ctcaagtaca gcttgcggct agcttcctag tttgctcttt gattttcatt gagtattaga
     7921 aaacaattat gttgatagag cttgggcaag agcccaattt cagcaaaaaa tgaagtaaat
     7981 cttctcataa taaaacgcat catatcaagc acgaaaattc cacgaggtca actacagtca
     8041 gaaagctgaa caacaagcca aaacgcccaa aaaaggcggc aaaaagcaag cacctgcaag
     8101 caacgtgccg aaatggtcaa atcctgatta tgtcaacgaa ttagacccaa aaatcgttga
     8161 tatgctagta gaatttcaca agtcacaagg cactttggaa actcccgagg cgcaagcaga
     8221 aatcgcccaa aaacgtgaag aaatcgagca aaggagagct gagcttgagg ataaaaaaca
     8281 agagcttttg aaccgcttga acaaatagag tttcgcaagt attatgctta caaattactt
     8341 gagcaattaa ctaaaatata aaccctgcct agatataaag gcagggttta tattttagaa
     8401 attcacgtag gttgttacgg tttttacata cccagtatag tttgagtttc tatagtattc
     8461 agtgataaac ttccattttc tttgagcaac atggatataa gtacttgtta tgtagtatgg
     8521 atatgggctt tgtgaatcca agtaagactg ataagcttgt ataccaaaat atgctccacc
     8581 aattattgca ccccatggac cccccaataa agcacctatc ctaccaatca tataactgat
     8641 tccagcacca gtcatgaagt tagcgaatgt gttagcttgt ttattcccat gtattgtgtt
     8701 gacgtaattc caaacattag gatcgtatga tctaaaagat atatttaggt cgatttcatt
     8761 cttttgataa gccatataaa atgccccatt gatatagacg ccgtcagcac gtcgttcaat
     8821 agtgtctaca cttccatctg gattgacaac ctcaagaact tcatcgctta aaatatttac
     8881 ttgcgtatct ccgaaccgca ctgatgagcc attctcaaac tgagcctcac cagatacaac
     8941 tttagagttt gccgataagc tatcatcagc aaaaacaaca agcgacgggg aaatgctaga
     9001 catacagaaa acagacataa ctagcaaaca catgcattta aacatcttag acataacgga
     9061 aactcttttg tatttttgat ttttttcaac ttttattata caataaaacc aaataaaaag
     9121 aaagcggtaa caatatgctt aatgcgaaaa ttttttatat atttttatgt ttgatcgtta
     9181 tcgaaactac aggcttgttg ttgttgaaaa gaggtctcga aatgggttat ttagacacag
     9241 aagctattat cctcgcagtt ttttcatttg ctttttacaa cctatgttca ttcgcttggg
     9301 tctgctctac aataaaaaat aaatagacgt attttcaaaa aaaaaaaaat gaatatttat
     9361 attagcaaaa tgacgattta aatcgtcgtt tttttgt
//
LOCUS       DAVLVF010000074         9349 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVLVF010000074 DAVLVF010000000
VERSION     DAVLVF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9349
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.074"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..1242
                     /locus_tag="VYN66_001622"
     CDS             1..1242
                     /locus_tag="VYN66_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV1495614.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKTPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            complement(1441..2853)
                     /gene="sufB"
                     /locus_tag="VYN66_001623"
     CDS             complement(1441..2853)
                     /gene="sufB"
                     /locus_tag="VYN66_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV1495615.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFNQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(2907..3347)
                     /locus_tag="VYN66_001624"
     CDS             complement(2907..3347)
                     /locus_tag="VYN66_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV1495616.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            complement(3334..4560)
                     /locus_tag="VYN66_001625"
     CDS             complement(3334..4560)
                     /locus_tag="VYN66_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1495617.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            complement(4571..5833)
                     /gene="sufD"
                     /locus_tag="VYN66_001626"
     CDS             complement(4571..5833)
                     /gene="sufD"
                     /locus_tag="VYN66_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV1495618.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            complement(5861..6631)
                     /gene="sufC"
                     /locus_tag="VYN66_001627"
     CDS             complement(5861..6631)
                     /gene="sufC"
                     /locus_tag="VYN66_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV1495619.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEGDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            complement(6781..6975)
                     /locus_tag="VYN66_001628"
     CDS             complement(6781..6975)
                     /locus_tag="VYN66_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV1495620.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            complement(7003..8658)
                     /gene="dnaX"
                     /locus_tag="VYN66_001629"
     CDS             complement(7003..8658)
                     /gene="dnaX"
                     /locus_tag="VYN66_001629"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV1495621.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            complement(8658..9155)
                     /locus_tag="VYN66_001630"
     CDS             complement(8658..9155)
                     /locus_tag="VYN66_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV1495622.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
BASE COUNT         2745 a         2082 c         1762 g         2760 t
ORIGIN      
        1 atgaaaaaaa tatttttaac tttgttaact gtctctcttt tagggggtgc ttctactgct
       61 gttgctcaag attttaccat tgccgctaaa catgcgattg ctgttgaggc aaatactggt
      121 aaaattctct atgagaagga tgcaacgcaa cctgtcgaaa ttgcttccat aacaaaattg
      181 attactgttt atctggtcta tgaagctttg gaaaacggaa gtattaccct ctccactcct
      241 gtagatattt ctgattatcc ttatcaattg acgacaaatt ctgaagccag taatattcct
      301 atggaggccc gtaattatac tgtcgaagag ttgcttgaag caactctggt atctagtgcc
      361 aacagcgccg ctattgccct agctgagaaa attgctggct cagaaaaaga tttcgtcgat
      421 atgatgcggg caaaactctt ggaatgggga attcaggatg ccactgttgt caatacgaca
      481 ggtcttaaca atgaaactct aggggataac atttacccag gttctaaaaa agatgaggaa
      541 aataagctta gtgcttatga tgtcgctatc gttgctcgca acctcatcaa aaaataccca
      601 caagtcttag aaatcaccaa aaaaccttct tctacttttg ctgggatgac aatcacttca
      661 accaactaca tgttagaagg tatgcctgct taccgtggtg gttttgatgg gctaaaaaca
      721 ggaacaacag ataaggctgg agagtctttt gttggtacta ctgtcgaaaa aggcatgaga
      781 gtcatcacag ttgttttaaa tgcagatcat caagacaata atccttacgc tcgatttaca
      841 gctacatctt ccctaatgga ttatatttct tctacattta cacttcgcaa aatcgttcaa
      901 caaggcgatg cctatcaaga tagcaaaacc cctgtacaag atggaaaaga agatacagta
      961 actgcagtgg ctccagagga tatctatcta atagaacgtg ttgggaatca atcttcccaa
     1021 tctgttcaat tcacacctga ttccaaagca atcccagcac cacttgaagc tggaacagtg
     1081 gttggccatt tgacttatga agacaaggac ttgattggtc aaggttacat caccacagag
     1141 cgccctagtt tcgaaatggt agcagacaag aaaattgaaa aagccttctt cttaaaagtt
     1201 tggtggaatc agtttgtccg ctttgttaac gagaaattat aaaaaatcgc gattttaaac
     1261 cgcgattttt tgatacaagt tcatcttcaa aatcaaagaa caaactagga agctagccgc
     1321 aggttgctca aaacactgtt ttgaggttgt agatgaaact gacgaagtca gtaaccatac
     1381 atacggtaag gcgatgctga cgtggtttga agagattttc gaagagtata aaatcaaatt
     1441 ttatccaact gatccctcca tttcatagct aatcaagcgg ttcagctcaa ctgcgtattc
     1501 cattggaagt tcttttgtaa agggttctac aaatcccatg acaatcattt cagttgcctc
     1561 agattctgac aatccacggc tcatgagata atacaattgc tcttctgaaa tcttagatac
     1621 tttggcttcg tgttccaaag ccacttgcga gttgtgaatt tcattaaatg gaatagtatc
     1681 tgatgctgac aagtcatcca tgataatggt atcacattca atgtgggaaa cagatttctt
     1741 agagttcttg ttaaaggtga cttgtccacg gtagtcaacc tttcctccac ctttagcgat
     1801 ggatttagac acaatagacg agctggtatg tggagcattg tgaatcatct tagcacccgt
     1861 gtcttggtgt tgccctgcat tagcaaaggc gatagagagc atggtaccac gcgctccttc
     1921 tccatcaagg taaacagatg gatatttcat agtcgttttg gcacccaagt ttccatcaat
     1981 ccactcaaca gtggcatcct tttgagcctt agcacgcttt gttaccaagt tatagacgtt
     2041 atcagaccag ttttggatag ttgtataacg catataagct ccgtccaaag caaaaatttc
     2101 tacaatggca gcgtgtaagc tattgcttga atatgttggt gctgtacatc cttctacgta
     2161 gtggacgctt gctccctcat caacgataat caaggtacgt tcgaactgac ctatattttc
     2221 gttattgata cggaaataag tttgaagtgg aatatctacc ttgacacctt ttggcacgta
     2281 gataaaagtt ccacccgacc atactgctga gttgagggct gccaacttgt tatctgtcgg
     2341 cggtaccaac ttcgcaaagt attgattaaa taagtctggg tattccttga gtgcggaatc
     2401 tgtatctgta aagataatac ctaatttttg gaactcttcc ttcatgttgt ggtaaaccac
     2461 ttctgactcg tactgggcag aagcccctgc taaataagca cgttcagctt ctggaatccc
     2521 gatacgttca aaggtttctt taatcttttc aggtacatca tcccaagaac gggctggttt
     2581 gtcagatggt ttttggtagt agattaagtc atcaaagtca atctctgaca agtctgctcc
     2641 ccaagtttgc atgggcattt ttttgaaggt ttcataagac ttcaaacgga actccaacat
     2701 ccactcaggc tcacccttag cagcagataa ttcacgaata acaccttcgt tgagtccttt
     2761 tcctgtcgat aagacaggct ctacatcgtc atggaaacca aatttatatt caccaaggtc
     2821 aattggtttt ggttctactc tttcttcagc cataatatcc tttctttctt catttctaat
     2881 aattcataag acaaaagaaa cttgtcttac tgtttttctt gattttcaat tgttttctta
     2941 agggcattcc aagctagggt tgcacacttg attctttgag ggaatttggc aacacctgac
     3001 aagaatgccg cgtctccaag ttggtcttga cgctcatctt tttgcccttg aaccatttca
     3061 gaaaaaatag tcgccagttc taaaatttct tgtttggttt ttcctaaaac ggcatctgtc
     3121 atcatactag cagaagcagt tgaaatcgtg catcctgaat ttagaaaagc aatatcttcc
     3181 aaacggtcct ctgcatcaaa cttgacagag agattgatga catctccaca agtcggattg
     3241 ttgagactga tttgctcagc gtcttctaac ttcccttggt gatgtggatt tttcgaatgg
     3301 tctgctacca ctgccatata aaggctatct agtttagaaa gtgccattga aaaactcctt
     3361 tgtcttttgt agggcatcga ctagtttgtc gcaatctgcc ttggtattat agatataaaa
     3421 acttgcacga gctgttgctg ggacttccaa atactgaagc aagggttgcg cacaatggtg
     3481 accagcacga acagccactc cttcataatc cagagctgtc gcaagatcgt gaggatggag
     3541 atcacctagg ttaaaggcaa taacacccga acgttgagcc aaatcctgag aaccgtaaat
     3601 ggtcaatccc tcaattgcct gcagttttgg atagacgtac gcaatcaatt cctgttcatg
     3661 agcttcaacg gcatccatac caatcttttc cagataatca actgcagtcg caagtccaat
     3721 agctcctgcc atatttggcg ttccagcctc aaatttccaa ggcaattcct tccaactagc
     3781 agattgctcg tagacaaaat caatcatctc gccgccaaat tctactggag acatttgctc
     3841 aagatacttt tctttgccgt aaaggacacc gataccagtc ggaccagcca tcttgtgacc
     3901 cgaaaaggcg aaaaagtcca gatccaagtc ctggacatca atcttcatat gaggtgtaga
     3961 ttgagcacca tccactacca tgattgcccc aacttggtgg gctaattgag tgatttcctt
     4021 gatcggattg accacaccaa gaacattgga ggcatgagct agggaaacaa atttaacctt
     4081 atcagtcaat ttagctcgca aatcctccat atccaaggca ccgtctttaa gatagacata
     4141 gacaagctct gctccagtct ttcgacaagc ttcctgccat ggaatgatat tagaatggtg
     4201 ttccattact gaaatcaaga cctggtctcc ctcagtgaga atttcctcag caaagcgtgc
     4261 cacccagtta aggctggttg tcgttcctct ggtaaagaga acttcctttg tagagcctgc
     4321 attaataaac ttacgaatgg tttcacgagc agcttcataa gaagctgtcg ctcgttccgc
     4381 taaggtatgg acaccacggt gaacattggc attgtcctgc tcatagtaac tgttaatagc
     4441 tttcagaact actagtggtt tttgtgtcgt cgcagcattg tccagataga ccagaggttc
     4501 atcattgaca atctgatcta aaattggaaa atccttgcga atcgcttcta catctaacat
     4561 aggcttcccc ttagcgtttt gacaatttct cttcgatagt tgcaatcatt tcatcacgaa
     4621 cttccttgac tggaatctcc acgataacag atccaaggaa accacgaaca accaaacgct
     4681 ctgcagttgc cttatccaat ccacgactca tgaggtagta catatcttct ggatctacct
     4741 gaccaataga ggctgcatgt cctgcagtga cgtcattttc atcaatcaaa agaattgggt
     4801 tagcatctga acgcgcttgg tctgaaagca tgagaacacg gctctcttgt tgggcatctg
     4861 ctcccttagc acccttgatg atgtggccga taccattgaa agtcaaagtt gctttttcaa
     4921 ggataacccc atgttgtagg atatttccga tagagttgca gccatagtta gttacacgag
     4981 tatcaatccc ttgtacctga cgaccacttg aaagagctac aaccttgagg tcagcatggc
     5041 taccattacc aatcaagtca ctatcaaaat cagcaacgac atttccttcg ttcatgacac
     5101 cgatagccca gtcaatactt gcatcgttgc ctaatttacc acgacggcta atgtaggcag
     5161 tgacgttttc acctagacgg tcaatagcag caaacttgac ttgcgcacca gaacgtgcaa
     5221 tcacttccac tgtgatattg gcagttgctt tgtcacttcc ttcaccgcgt gactctaaac
     5281 gctccagata actaatctta gaatttttac cagcgataat cataatatgc ttgttaaacg
     5341 gcacattgct atcgctatct tggtagaaaa ttccttcaat tggctctgtg atttctacgt
     5401 tatctggaat atagagtaca gcaccactgt taaagtaagc tgtgtggtaa gccgccaact
     5461 tgtcatcatc atacttaaca gatgacatga agaattcttc gatcagctct ggaatttctt
     5521 ctaaagctga gtgaaagtct gtgaagacaa caccctgttc agctaactca actggagttt
     5581 gttcgaaaac agtttgagtt cctacttgca ccaacttcaa gtgatgatct aaagctgtga
     5641 aatctggaac atttgctgat ggctcatttt ctgtaatcgt tccatcaccc agattccaac
     5701 ggtggaattt gacacgctca ataactggta attccaaagt ctcaatcttg tcaaaagctt
     5761 tttgacggag atcagccaac cagcttggtt cagcgtgcat ttctgaaaaa agtttaatat
     5821 tttctctagt catttacttc tcctaaaaga tacgaggaaa ttacaattct tccttgtagt
     5881 cgtagccaag ttcttcagct aattttgcgt atccttcacg ttccaaacgc gcagccaatt
     5941 ctggaccacc agaaaggaca acacgacctt ccatcatcac gtgtaccaca tcaggtgtga
     6001 tatagttcaa aagacgttgg tagtgagtga tgatcatagc accaaaacct tcaccacgca
     6061 tggcattgac acctttagac acaactttaa gagcgtcaat atcaagacct gagtcaatct
     6121 cgtccaaaag agcaaatgtt ggctccaaca tcaaaagttg aagaatttca ttgcgttttt
     6181 tctcaccacc agagaagcct tcgttgaggt aacgctctgc catttcttct ttcatgttga
     6241 gcaattccat tttttcatct agcttagtaa taaactcacg aactgaaatc ttctcatcac
     6301 cttctttacc cgcattcata gcggcacgaa gaaactcagc attggtaatt ccagggattt
     6361 ctgatgggta ttgcatagca aggaaaagtc ccatacgcgc acgctcatcc acttccaact
     6421 caaggatgtt tacgccatca aacaaaactt cacctttagt tacttcatag tttggatttc
     6481 ccatgatagc ggcagaaaga gtcgatttac ctgtaccatt tggtcccatg atagcggcaa
     6541 tttctcctgt tttcagggtc aggttaaccc cttttaaaat ttcttttcct tcaatctcaa
     6601 cgtgaagatc tttaatctct aatactgaca tgatagttcc tttctttttc aaaactttta
     6661 cagtacttac tagaaaaacg tctgatttcc ccagaaatag agcaaaaatt ctataaatat
     6721 acttttctag tataacaaaa aaaagcctaa aaggctttga ttttgtctgg aagctgaggg
     6781 ctagtctttc ctattaaaat gctgatcgat gacatcaacg attttgccca tcacataact
     6841 gactcggaaa tttctaaaga gtaaaattgc ccaaaaggct gccataatat agcggccagt
     6901 catccaggct tcattgaaaa aataggtctc agcagctgta aacagcgcca taataataaa
     6961 ttgttgttta ttcataaact tattgtatca tgaaatcttt ctttagtctt cctctacctt
     7021 cactttatca gccaaaaatt caaatccttc tggaatcagg ctttcttcta cttctttttc
     7081 agtttgagaa gatttggctt tggctgaaaa ggctgcgcga acttctttcc attcctccat
     7141 ggaaatagct aaaatctcag gtgaaaaacc tgccgcctga ctgaggatat taccaaacat
     7201 ggtattgaga ttgtctcgtt tcatagtttg accagcattg aagttagact caaaagcaag
     7261 aatagcatgg tgttcattgg cagcaaccgg ttgagaacca actagcagag ccttgtccgg
     7321 cccacctaga ctttcaatta cctctcccca ggcattctgc aaacgaatta gattttgacg
     7381 tgctaaatca ggattttcga cggcctcttg taagatagat tgcactttat tgcgatcgac
     7441 acgatagact gttttgcccg tagctggtcg actaggagct ggtgcaactt gtttaggaac
     7501 cgcacctaca ttagaaagct cttgtttgag acgggcaact tcctgtctca gcgtagcaat
     7561 ttcattttca accgctcctg atagagctgg ttcgggcttg atttccgcca aacggacggt
     7621 catcatttca gcataaatct tgggctgcaa actagactta atatctgcta aactcactgt
     7681 tgctaagcga atcatttcaa acagattttt ttgaggaagt gccaaatttt ctacaaagac
     7741 tgaactatga tgagtatttt ctccccctgt ttgaacaatt aacaagtctc ttaaatagtg
     7801 caaaagatcg gtcacaaaac gagtcatgct cttaccattg tcaaaaagaa gattcaagca
     7861 agacaaagct ttgggaacat cctgttgaga caaggccgcc acataatcat ccaaggctga
     7921 taggctaatg gtgccagtaa tttcttcaga gatagcagtc gtcagctcat ttccctgtgt
     7981 caaactcagg gcttgatcca aaatagacaa ggcgtcccgc attccacctt ccgcccgcct
     8041 ggcaatgatt tccacagcct ctggttcaga actgatattt tctttttcta agatatagtg
     8101 aatatgttcc ttaatatcct gtgtcttaat tgatttaaac tcaaaacgtt gcacacggga
     8161 tagaatagta gcaggaatct tgtgcaattc agtagtggcc aaaataaaga ctacattctg
     8221 tgttggttct tccagcgtct ttaggagggc attaaaagcc cctgtagaca gcatgtgaac
     8281 ctcatctatg atataaacct tataacgagc aaggctaggc gcataggtag atttatcacg
     8341 aatttcgcga atttcatcta ccccattatt agaagctgca tccatttcaa tgacatcttc
     8401 taaactaccg tccgtcactg cttgacaaat atagcagtta ttgcaaggtt cgccacccac
     8461 ttgattggga cagttcatag ccttggcaaa gattttagca acactggttt ttcccgttcc
     8521 acgaggacca gaaaaaagat aagcgtgact tattttctct tgctccaccg cttgtttaag
     8581 agtcttagcc acaacttctt gaccaactaa ctgggagaag ttttgacttc tatattttcg
     8641 ataaagtgct tgatacatta agatttttcc tcaaacatcg taaagtccca tgttgtcttt
     8701 tcaagcaaaa tagcgacaaa ttgttccaaa taatctcgat ccatagcatc gtaatcctca
     8761 atctctgaag aatccagatc cagaactcca agtaactgac cattcttcat catcggcacc
     8821 acaatttcac ttttagctag actatcacaa gaaatatagt tgagataggt cgtcacatct
     8881 ccaacaataa cagtttcctg aaagtgagct gcctcaccac aaacaccctt gcctagtgca
     8941 atacggatgc aggaaacacc tccttggaag gggcctaaaa ccaattcctt tccatcgaac
     9001 aaataaaagc ctgcaaatac ggtattagga aaacgtgatt ttatgagagc actggcgttg
     9061 gaaagattag ccaaaacatt ggtttcgcct tccaatagga aggacaattc ttcatttaac
     9121 atttgataac gtgattgttt ttctgatttt aacatagaac tattatatca aaaaaggagc
     9181 agagacaaaa gaaaaatccc ttgtttagta aacaaaggat tttgggctct ataatatttg
     9241 tagtgggtaa atcccctatg gatattatgg agcctatttt gtgtagaaaa aaagtcccat
     9301 atgacctata atgaaaagca acaaaacaac tcattagaaa gaatcatat
//
LOCUS       DAVLVF010000075         9077 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVLVF010000075 DAVLVF010000000
VERSION     DAVLVF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.075"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(405..2684)
                     /gene="pulA"
                     /locus_tag="VYN66_001631"
     CDS             complement(405..2684)
                     /gene="pulA"
                     /locus_tag="VYN66_001631"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV1495623.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(2796..4754)
                     /gene="ligA"
                     /locus_tag="VYN66_001632"
     CDS             complement(2796..4754)
                     /gene="ligA"
                     /locus_tag="VYN66_001632"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV1495624.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVADSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(4847..6025)
                     /locus_tag="VYN66_001633"
     CDS             complement(4847..6025)
                     /locus_tag="VYN66_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1495625.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LNMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(6105..6968)
                     /locus_tag="VYN66_001634"
     CDS             complement(6105..6968)
                     /locus_tag="VYN66_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1495626.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQKAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(6971..8872)
                     /locus_tag="VYN66_001635"
     CDS             complement(6971..8872)
                     /locus_tag="VYN66_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1495627.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKYSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         2735 a         1999 c         1624 g         2719 t
ORIGIN      
        1 acgtcaaggc gaggctaatg tggtttgaag aaattttcga agagcgtgaa tgagtatcat
       61 ctatagtaaa ataaaaaaac tgaacaattt ggttggggac agccaaacca atttctcaca
      121 atgtttcaga aacaagggtg tgctactcca atttcagcct actataactg tcatagattg
      181 ctgaaacaaa gtctaggtaa aagtcttcat aataaaaaga cctcctatca agtgttcaaa
      241 aactttgata ggaggtcttg ttttgtgaaa atatttatca aattttctat acaagtgagc
      301 tgttagccag gttctttcta ttctttcaat ttcaatgaat ggatttttta ctaatactca
      361 taactgggaa tttgtctgtg taaaaatagc gagatagatg gtatttataa aacactcaag
      421 acagctagac taatatcatt taaaacatta tcttcttttg agcgactgtt ggttaccaac
      481 atagctaaat ttcctgcatt ttcaaattga tagggttctg atttagcatt cacaaccacc
      541 aagaggtgtt ctttgccgtg aacttcatag ataaggtagc cgctatgttc aatcgcagaa
      601 tgcacaaaga catgatggta aatttcatca tagctagagt aagaaaaggc accagttttt
      661 gtcttcaatc ggatgacttg acggataaac tcaatactgt cttgacgctc attaatcaag
      721 ttccagttca cttggttcac actgtcagga gcattatagc tattcatcgc acgctctcta
      781 tcatcatggg tcaactcacc attttcacca gtcgcaacca gtttggtacg accaaattct
      841 tgaccgattt ccataaaggc catcccctgc atgagcagat tcatggctgt ggcagtttcg
      901 accttgcgca tgatttgctc tgaactttgg tctggatgaa gggttgccaa taaatcgtga
      961 agattgtaat tgtcatgggc ttctacatag ttaagcacct gatttggatg tgtatagctt
     1021 cctaattcac gacttcctag gattgcttta gctagaattg gctctgtcgc agcaccactg
     1081 acaaaacctg acttgatagc accataaact tctccccctt tgacagcatc gcgctgattg
     1141 tcattaaaga aaccaatatt tggcatctgg taggcattgt ccttcttggc cttatcataa
     1201 ggggcaagac ctgttcccat atcccatcct tctccataga ggataatgtt ggagtcgatt
     1261 tcatccaagc tttgacgaat catctgcatg gtcttgacat catgaatccc catcaagtca
     1321 aaacggaagc cgtcaatatt atattcctgc acccagtata gaagagaatc aatcatatac
     1381 ttgcgaaaca tttcgtgttc actggctgtt tcatttccaa cacccgttcc attctggaag
     1441 gtaccatctg gattcatacg ataatagtaa tcagggactg ttgtttggaa tggtgcatca
     1501 acaactgaga aggtatggtt atagactaca tccataatga ctccaatacc cgcatcgtga
     1561 taagcttgaa ccatcacctt caaatcacga atgacctgag ctggatcatc tggattagtt
     1621 gaaaaactag tttctggcgc gttatagttt tgtggatcat aaccccagtt gtaggttaca
     1681 tttccatcct catcgtattc tttatgacgg tctgcaattg gttgcaattg aacataattg
     1741 tagcccagct tcttgatgta atcaaaagca gttgactggc cgtattggtt aactgttcca
     1801 gcctgagcag cacccaagaa agttcctcga agatgttcat ctacacccga tgtaggtgat
     1861 ttagtcaaat cacgaatatg catttcacag ataactgcct tacatggatt ttccaagcgc
     1921 caagtagcct ccgaaccgtg cttaacctcg aagttttcaa cttgcttttc tacatggctc
     1981 agaatagctg aacgtttgcc atcagggctg gtcgcgattg tataaggatc acgtgtcagt
     2041 gtttggtgat gagggaattg gacttgatac tgataagcct tacctaccaa atcttcctca
     2101 acatccaaac tccagacacc gattgtattg tccttatgat tataagagta gctattgcct
     2161 cttttcatct caaaagtctt ccaaacgggt gcatcattag cagctgattc ataaacgaca
     2221 acttgcactt ctgtcgctgt aggtgaccag agggaaaaat gagcctgatt gtcctctaca
     2281 cggcaaccca attctccttg gtaaccccaa tgatggtcaa aactagcact gttaatggcc
     2341 ttatcaaagg caaaaggatt ttgattttta tagaaaggac tggcaatagc aggattttca
     2401 gagtaataaa tcctatcatc gccttccaaa atccagacct ctgttaatag gggatagtga
     2461 ttaaaacgga tagaatattc tttactagtt tgacctgtat gaaccacaaa attcaagctt
     2521 tctataacat gtgaacttgg gtgttcaaag ctaaataaag ctccaaaata atcttctttg
     2581 taggttagca aatcaattcg ttgatcctga ctttttacaa aggagcaagt gtcatattct
     2641 ccattcttac gatggtaatg aatgcgcata gggtagttat acatttttta tttttccttt
     2701 ttactttgtt tctatttcac taataaattt ttgtcaatct cgtctcaatt aacagacata
     2761 gtcatattct ctaaactctg tttttaaacg atccattaca aactttctag ccatgcctca
     2821 tctctgacct ggataccaag ttcttgtgct ttttgcagtt tacttccagc gtctgcacct
     2881 accacgacga ggtcggtctt tttagaaata ctacctgtca ctttggcacc cagactttcg
     2941 agtttacttt tagcttctga gcgcttgagt cgttccaatt ttcctgtcaa taccacggtc
     3001 aaacctgaca aggccgcatc cgctactacc gtctgtcctt tatagtccag attgacccca
     3061 gtttctttca attctctgag cagaatttca gagccttctg tcgcaaaata agtctgaaga
     3121 cttttggcaa tcacgccacc tagactttca atactagcca cttcctctga atctgcctga
     3181 gacagatttt caattgaatg gaaatattga agtaaaagct gactaacctt gcttccgaca
     3241 tgacgaattc ccaaaccaaa taagagcttc tcggcagaat tttcctttga tgcttggata
     3301 gcctgataca gtttagcagc ggacttttcc ttaaccccct ctaaaaggag gaaatcctct
     3361 tcttgcaaac gataaatatc cgccacatcc ttgactaaat tagcagcaaa aagcttctca
     3421 acaatagatg gaccaaggcc tgtaatattc atagcatcac gagaagcaaa gtgaatcaag
     3481 ccttccatga tttgagcagg gcaacgcgga ttgatacaac gtagggccac ttcatcttca
     3541 aagtgcaaca agtcagagtt acaacttgga cagtttgtag ggatatctag tttttcttca
     3601 gaaacccgtt tggactctac cacacgtaaa acggcaggga tgatgtcacc agccttatat
     3661 acaatgaccg tatcgtcttt tcggatatct ttttcagcaa tataatctac attgtgcagg
     3721 gtcgcacggc taacagtcgt accggcaagt tgtactggtg ttagattagc agttggagtt
     3781 acaacaccgg tacggccaac tgtccagtca actgataaga gttgagcttc tttttcttcg
     3841 gcagggaact tgtaggctac tgcccacttt ggagccttaa ctgtaaaacc aagttcttct
     3901 tgacttgcta ggtcgttgac cttgattacc actccatcaa tatcgtaagg cagattttcc
     3961 cgttcctgtc ctacttcttg gataaaattc cagatttcat ctatgttttc agccaagatt
     4021 cgcttaggat tgaccacaaa acctagttgt tctaggtact tcaaaccctt ttcttggcta
     4081 tcacgagttg aagggctggc ttcttgatag agaaacgttg caagattacg cttggcaact
     4141 actgctgtat ccaactgacg cagagttcct gctgccgcat tacgaggatt agcaaattca
     4201 ggctctccat tttcttggcg cacttggtta acttggtcaa aggaagcgcg tggcatgtaa
     4261 cattccccac gaactgtgat atctagttct tctggcaaag tcaaagggat gtccttaaca
     4321 cgcttgaggt tttctgtgat attttcacca attgaaccat ctccacgtgt taccccagca
     4381 accaaaatcc ccttttcata agtcagcgag atagataagc catcgatttt cagctcacaa
     4441 atataggtcg gatgagccac ttccttacga acacgcgcat caaaagcatc tagctcctca
     4501 cgtgaaaaag catcctgcaa actataaaga ggatactgat gactgtattt ttcaaaacca
     4561 tctaaaacct tgccaccaac acgatgagtc ggactgtctg ctagcacttg ctctggataa
     4621 gcagtttcta actcgaccaa ctcacggtaa aggcggtcat actcactgtc tgcaaccgag
     4681 ggattatcgc tggtatagta ctcagtcgca tagcgattga gcaaagcgac taactcattc
     4741 attcttttat tcataagacc attttaccat aaaacaagcc ctcctcacaa acgagaaggg
     4801 cggaaaaaac acttagtttg aaattatttt tgaaactcaa gcaaccttat atcaattttt
     4861 caaaatgagt tcgaacataa ataaacgata tacaagacaa gatgataaca ccacttccaa
     4921 ttatcaggaa agaagagaga tgtacacttg gcaagactgt cataaatcct tttgcaatag
     4981 gcataaatag aatagctaag gtaaaaattg tactcagtac tcttccaaga aattcgctct
     5041 caaccttggt ttgtacttga gtaaaaaagt gaatattaaa aatcgtcata aacaattcac
     5101 aaactaaatt tccagaaaag gaaagaaaag ttggaagtgg taatcccatc ataaaaactc
     5161 cgacacctgt caaagccagt aaaatcaaaa gattataaat attagcttta attttactag
     5221 ctagaagagc cccaatgatg gaaccaatag cccccatagt taaaatactt gcataggctc
     5281 cttctgaccc gtaaagctga ttcgaaaagg gaagtagaaa ttcaaaagct gcaaaaaaga
     5341 aattaacgct ggaagctacc agcaaaagga agaaaatttc ttgctgatgc cagatatagt
     5401 gtaacccatc cttgatatct acaaaaatat ctctcccagt aaaagccttt ttctcttgaa
     5461 cttttgcttc ctcttttgga aggaaagcca ctagaacaaa agcaatgaaa aaagtcagcg
     5521 agtctagcag tagcgtcata ttgagacttg caaactgtaa aacaaggaag gaaagaacag
     5581 gagagctaac acctacaacc tgcaaaacca gctctaagcg agaattatag atcacaatct
     5641 catctttctc caccacttca gttatgatag ctttattggc tgtgcgagaa aaggcaaaag
     5701 caatagcctg cacaatgtta gcaacaatca aagcgccaat catccagcta tcattcctta
     5761 tgaaagaaat agccagacaa agaatcccac aaacaagatc tgccgtcatt aaaatcttac
     5821 gacgagaaaa acggtctgaa ataactccgc caaagggatt gacgagaata gatgtgacga
     5881 gctcagaaat ctgatacatt cctaaaactg tctgtcctat agtccccata gaagccaacc
     5941 agacactatt tccataatca tagagcatat ttcccatttt attgatagcc ccacggctaa
     6001 tcaactgcac tgcatagcga ttcatattaa agctcctctc aaattttgaa actattgtat
     6061 caaaaccgaa aggagctttt tattttttcc cttatttggg aaaattaact tttgacaaat
     6121 ttttcgtagt gttcctgata ataggctact tgctctggaa gacctaacac atcaaaaata
     6181 tgcatggcct tttgcatctg cttacagcct tctttacact gtcctttttg atataaggca
     6241 aaacctttta aataatggaa aacattacgc tcataaagct taataccttt gtcaataatc
     6301 ttctctgtat aagcctcaaa atagttggca ttataaaaag aagaatgctc taaacaatgc
     6361 tggtaacaat tgagggccaa aatcaaaact aatctcttat ggcgactaat ctcttggtaa
     6421 aattcctccc tctccataac ttctctacca atccgagtga catagtctac atcgtagaaa
     6481 ctatagaggt taccgaaaag aatcaactca tacatggtcc attcttctgt tttgaagaga
     6541 taatctgcta ccttatccaa atcatcctgc ttcatatcat aagtcgcatc tctttgacaa
     6601 atcagacctt gtagcaaaat ccagttcagc tcaaaataaa gggaagtcgt cgaactctta
     6661 gacttttcaa gttgttctct ttgaagcttt tgaaaacctg caatatcgtt tgaatagtaa
     6721 agtgggataa tctgtgccat catagacaca tgttcatgat tatgaaaatt ccttgcctta
     6781 tccatgaaat tttcgattgt tacatgaatg ttatccaaaa tctcaaagaa acgggagact
     6841 gccaggtcag actccccaag ctcaaagcga gataactgag aggtagagca ggattcgcct
     6901 gctgcttcct ttaaagaata atttccactt gttcgaaatt cacgaaatac ttttccaaga
     6961 tgttccatct ttacacctga tctgataatt cttcccactc aagcatagct tcttcctgac
     7021 gatggctgat tttgtccagc tcagcctgta attccatgag tttgtcggca tcgtttgttt
     7081 ccaacatttg ttcagaaatg gcttggcttt gactttctag ctcttcaatt tcagcttcta
     7141 gactttcgat ttgtcgcatg agtttgcgaa cttctttttg actttctttc tgggcctgat
     7201 agtcattgac tggacttgct tcctttgctt gattgctagt tgaagcttcc tcagtctgac
     7261 tcatttctgc tgttgctttc ttctcaacat agtagtcgta atctccaagg tagagagttg
     7321 aaccattctc agacaattcc aaaacatgag ttgccacacg attgataaag taacgatcat
     7381 gactgacaaa aagcaaggta ccatcaaagt caatcaaggc attttctagc acttccttac
     7441 tatcaatatc caagtggttg gtcggctcat ccagaatcaa aaagttattg ttttccatag
     7501 acaatttagc taaaagcaaa cgagcttttt cgccaccaga tagcatgccg actgattttt
     7561 taacatcatc tcctgagaaa aggaaggccc caagacggtt gcggatttca acttctggtg
     7621 tcagtttgaa atcattccag agttcatcca gcaccgtatt acttggtgtc agcttgcttt
     7681 gggtttggtc atagtaacca acctcaacat tagcgccaaa gcgcttttca cctttgataa
     7741 aaggaatctg gtccacaata gacttgataa aggttgactt gccgatacca tttggaccaa
     7801 cgatagcgac agcattcatc ttacgaagat ctaggttgat aggttgtgac aaaacttccc
     7861 cgtcataacc aatagcagta ttttcaacag tcaaaacaac attgcccgac gttttttcag
     7921 actggaaggt catgttggct gctttcttgc cagcttcagg cttgtccaaa cgctccattt
     7981 tttctagttg tttacggcga gattgagcac gtttggtcgt tgaagcacga actagattgc
     8041 gattgacaaa gtcttccaga gcagcgattt ccttctgttg cttttcatag ttttttgcct
     8101 cagtagctag cttttgctcc ttcaactcaa caaaacgaga gtaattcccc acatagcgat
     8161 ccaaggaata cttggtcaaa tctagcgtaa ttgtcgcaac cttgtccaag aaataacggt
     8221 cgtggctgac gataatgagg gcaccgctat agtttaccaa gtaattctct agccaggaga
     8281 tggtttcaat atccaagtgg ttagttggct cgtccaagac caagagattg ggcttttcaa
     8341 ggagcatttt agctagagcc aaacgagtat tttgaccacc agaaagctca gcaattttca
     8401 tctgccacat agactcgtca aacttgaatc cattcaaaat cgctcgaata tcagcttcat
     8461 aggtaaagcc acctgcttgg cgaaaatttt cagataagcg gtcataatct gacatcagtt
     8521 tatccaaatc ctcaccagac ttttcaccca tctccagctc catctgacgc agttgtttat
     8581 ctgtccgacg taagtcatca aagacatgaa gcatctcatc atagatggta ttttcagact
     8641 caaaacggct atcttgagct aggtaagaca gagaaatatc ttttttctta ttgatttctc
     8701 cactagttgg ctcctcttct ccaactaaaa tcttcaaaag agtagactta cctgcaccat
     8761 ttttcccaac aagggcaatc cgatctcgtt catcaacctg caggttgata ttatcgaaaa
     8821 gaacctctcc tgcaaaagaa cgttcaattt tattagcttg taaaataatc atacaagtag
     8881 tatagcatgt ttccctaagg cattcaagat aatcgtaagt cttttagtac aacttttata
     8941 acataaaata aactaaatta tgtatatttt atattagatt ccttcactat cttgttggat
     9001 tttctaacca gctaatcttg tttcaaatag ttatcgcaca agcctattat ttaattcttt
     9061 tcattattta cgtacgc
//
LOCUS       DAVLVF010000076         8964 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVLVF010000076 DAVLVF010000000
VERSION     DAVLVF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.076"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            122..277
                     /locus_tag="VYN66_001636"
                     /pseudo
     CDS             122..277
                     /locus_tag="VYN66_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            477..3752
                     /gene="rexB"
                     /locus_tag="VYN66_001637"
     CDS             477..3752
                     /gene="rexB"
                     /locus_tag="VYN66_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV1495628.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGKQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3749..7399
                     /gene="addA"
                     /locus_tag="VYN66_001638"
     CDS             3749..7399
                     /gene="addA"
                     /locus_tag="VYN66_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV1495629.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKANFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7659..8837
                     /locus_tag="VYN66_001639"
     CDS             7659..8837
                     /locus_tag="VYN66_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495630.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2772 a         1875 c         1902 g         2415 t
ORIGIN      
        1 tagattgaaa taagatacga acagcttgat tagggaaatc aaattaattt ctagaaatat
       61 tttagcatcc acggtgtact attatagttt caatttacta tagttcaatc attctatcaa
      121 gaatattgaa tatataaagg cacgtaatat taaaacttct tctgatatcg aattagagtc
      181 cgatgttgat ggcgataaat cagacaatct accagtcgac attaagatac tgggacaaca
      241 cattgaagtg tattccgtac ctaagaaaca atcataaaac tcaaagcctt gttaccgagg
      301 cttttttatt atgtgatttt cagtagccag acttattcta tctaatacaa gtaaagctaa
      361 gtatttaaga tttagcagat gatgaagaaa agaaaggatg gcctaaatat tttaacagat
      421 ttacgaggct aatcatggta taatataggg tgatgaaaaa ttccaacgag gctgagatga
      481 aattacttta tactgatatt cggacttctt tgacagaaat tctaacaaga gaggcagaag
      541 agctagttgc agctggcaag cgggtcttct acattgcccc caactctctt tcttttgaaa
      601 aggaacgcgc cgtgctggaa tgcttgtccc agcaggcttc tttttcgatt accgtcacgc
      661 gctttgctca aatggctcgt tatctggtct tgaatgattt accagctaaa actactcttg
      721 atgatatcgg tcttgggttg gccttttaca aatgccttgc cgaactcgat cccaaggact
      781 tgcgtgttta tggcgctatt aagcaggatc ctcaattgat ccagcagtta attgagcttt
      841 atcatgagat gaccaaatcc cagatgagtt ttttggactt ggagaattta acagatgagg
      901 ataagagggc ggatttactc ttgatttttg agaaagtaac agcctatctt aatcaaggtc
      961 agttagccca gggaagtcag ttgtcccatt tgattgaggc tattgagaat gacaaggtaa
     1021 gtagtgattt taatcaaatc accttggtca ttgacggctt tactcgtttt tctgctgagg
     1081 aagagcgggt tgtggactta cttcacggca aaggtgttga gattgttatc ggggcttatg
     1141 ctagtaagaa agcctatacc agtcctttta gcgagggcaa tctctaccaa gccagcgtaa
     1201 aatttctcca tcatctggct tctaaatacc aaacgcctgc tcaggactgt tctcaaactc
     1261 atgagaagat ggatagtttt gacaaggcct ctcgtttgtt ggagtcttct tatgactttt
     1321 cagaactcgc tttggatgtc gatgagaaag accgtgaaaa tttacaaatc tggtcttgtt
     1381 tgacgcaaaa ggaggagttg gagctagtag cccgtagtat tcgtcagaaa ttacatgaga
     1441 actcagacct gagctacaag cattttcgta ttctcttggg ggatgtagct tcttaccagt
     1501 tatctctcaa aaccattttt gaccagtatc agattccttt ttatcttggt agaagcgaag
     1561 ccatggctca tcatcccttg actcagtttg tcgagtctat tttagcttta aaacgttacc
     1621 gttttcgtca ggaggatttg attaatcttc ttagaactga tttgtatact gacctcagtc
     1681 agtctgatat tgatgctttt gagcaatata tccgctatct tggtatcaat ggcttgccag
     1741 cctttcagca aatcttcacc aaatcccacc atggaaaatt taatcttgag cgtttgaatg
     1801 tcctccgcct gagaatttta gcacctcttg aaaccctctt tgccagccga aaacaaaagg
     1861 ctgaaaacct cctacaaaaa tggagtgtct ttctaaaaga aggagctgtg accaagcagt
     1921 tacaagattt gacaaccact ttggaagctg tagaacagga aagacaagcc gaagtttgga
     1981 aggctttctg ccatgtttta gaacaatttg cgactgtttt tgctggttca caggttagtc
     2041 tggaagactt cctagccttg ctccattctg gaatgagttt gtcccaatac cgtaccattc
     2101 cagcaacagt ggacactgtt ctggtgcaga gttacgattt gattgcacca ttgactgctg
     2161 actttgtcta tgctattgga ctaactcagg acaatttacc aaaaatttct caaaacacca
     2221 gtcttctgac agatgaagaa aggcaaaacc taaaccaagc gaccgaagaa ggcgttcaat
     2281 tactgattgc cagcagtgaa aatctcaaga aaaatcgcta cactatgctt tccttggtca
     2341 attctgctcg taagcagttg ttcttgtcgg ctccaagcct ttttaacgaa agtgaaagta
     2401 aggaatctgc ctatcttcaa gagttgatcc attttggatt taggcggaga gagaagagga
     2461 tgaatcacaa aggactgtct aaggaggata tggggtccta tcacagtctt ttgtctagtc
     2521 tggttgccta tcaccagcag ggtgagatga gcgatactga gcaagatttg acttttgtca
     2581 aggttctgtc gcgtgtcata ggtaaaaaac tagatctgca aggtctggaa aatccagcta
     2641 tcccaaccag tccaagcagc aagaccttag ccaaggacac cttgcaagct ctctatcctg
     2701 ccaaacagga gttttacctg tctacgtcgg gtttgacaga gttttatcgc aatgaataca
     2761 gttatttcct acgctacgtt ttaggcttgc aggaggaatt acgtttgcgt cctgatgccc
     2821 gtagtcacgg gaatttcttg catcgtatct ttgaacgcgc cttacagttg cctaatgaag
     2881 attcctttga ccaacgtcta gaacaagcta ttcaagaaac cagtcaagaa cgcgaatttg
     2941 aagctattta tcaagaaagt ttggaagccc agtttaccaa ggaagttttg cttgatgttg
     3001 cacggacgac tggacatatt ctccgacaca atccagccat cgaaaccatc aaagaagaag
     3061 caaattttgg tggaaaagac caagccttta ttcaattaga caatggacgc agtgtctttg
     3121 tacgaggcaa ggtggaccgg attgaccgtt tgaaagctaa tggagcgata ggagtagtag
     3181 actacaaatc cagtctgact cagttccagt ttcctcattt ctttaatggg ctcaattctc
     3241 agttaccaac ctatcttgct gccctaaaaa gagaagggaa gcagaacttt ttcggcgcca
     3301 tgtacttgga aatggctgaa cctgtccaat ctctgatggc ggtaaaaagt ctggcaggag
     3361 cagtggtaga agccagcaaa tctatgaaat accaagggct cttcttggaa aaagaaagca
     3421 gttatttagg cgaattttat aacaaaaaca aggctaatca actgacagat gaggaatttc
     3481 agctcctact ggactacaat gcctatcttt acaagaaagc tgctgagaag attttagcag
     3541 gccggttcgc catcaatcct tatactgaaa atggcagaag cattgcccca tacgtccagc
     3601 aacatcaggc tattacaggc tttgaagcca attaccatct gggccaagcc cgtttcctag
     3661 aaaaattgga cctagctgat ggcaagcgtc tggtcggaga aaaactcaag caagcttggt
     3721 ttgaaaaaat aagagaggag ttaaatcgat gaagcttatt ccctttttaa gtgaggagga
     3781 gattcaaaaa ctgcaagaag cagaagcaaa ttcgagcaag gaacagaaga aaactgccga
     3841 acaaatcgaa gctatctaca cttctggtca gaatatcctg gtctcagcat cggctggttc
     3901 tggaaagacc tttgtcatgg cagagcgcat tctggaccaa ttggcgcgtg gtgtcgaaat
     3961 ttctcaactc tttatctcaa cctttaccgt caaggctgca actgaactta aagaacgttt
     4021 agagaaaaaa atcagcaaga aaatccaaga aacagatgat gtcgatctca aacaacactt
     4081 gggtcgccag ttggcagacc tacccaacgc tgccatcgga accatggact ctttcacaca
     4141 aaaattcctt ggcaaacatg gttatctgct tgatattgca cctaatttcc gtattttaca
     4201 aaaccaaagc gagcaacttc ttctcgaaaa cgaagtcttt catgaggtct ttgaagcgca
     4261 ttaccaaggt aaacagaaag agagctttag tcatttgctg aaaaactttg ctgggcgtgg
     4321 caaggacgaa cggggtctgc ggcagcaagt ctataaaatc tatgacttcc tccaatccac
     4381 cagtaatcct caaaagtggc tgagtgaatc tttcctcaaa ggctttgaga aagctgattt
     4441 taccagtgaa aaagaaaaac tgactgagca aatcaaacaa gccctctggg atttggaaag
     4501 ctttttccgt taccatctgg ataacgatgc caaggagttt gcaaaggctg cctatttaga
     4561 aaatgttcag ttaattctgg atgaaattgg ctccctaaat caggagttcg atagtcaggc
     4621 ttatcaggca gtacttgcgc gtgttgtcgc catctctaag gagaaaaacg gtcgtgctct
     4681 gactaatgcc agccgtaagg ctgatttgaa gcccctggct gatgcctaca acgaagagag
     4741 aaagacccag tttactaaac taggacaatt atcagaccag atagcgattc tcgactatca
     4801 agaacgttat catggagaca cttggaaact agctaaaacc ttccaatctt tcatgagcga
     4861 ttttgtagag gcttatcgtc agagaaaacg acaggaaaat gcctttgaat tcgctgatat
     4921 cagccattac accattgaga ttttagagaa tttcccacaa gttcgtgagt cttatcagga
     4981 gcgcttccat gaagtcatgg tcgatgagta tcaggatacc aaccatattc aagaacggat
     5041 gctggaattg ttgtctaatg gccacaatcg ctttatggtg ggagatatca agcaatccat
     5101 ctatcgtttc cgtcaggcag acccgcagat tttcaatgag aaattccaac gctatgcgca
     5161 aaatccccaa gaaggcaagc tcattctcct caaggcaaat ttccgtagta gttcagaagt
     5221 gctgtcagca accaatgatg tctttgaacg tctcatggac caagaggtcg gcgaaatcaa
     5281 ctatgataac aagcaccagc ttgtttttgc caataccaaa ctgactccca atccagacaa
     5341 caaggcagaa tttctcctct acgacaagga cgatacaggt gaggaagaag agagtcaaac
     5401 agaaacgaaa ctaacaggcg aaatgcgctt agttatcaag gagattctga aacttcatca
     5461 agaaaaaggt gttgccttta aggaaattgc ccttctgacc tccagccgca gtcgtaatga
     5521 ccagattctc ctcgccctgt ctgagtacgg aattcctgtc aaaactgacg gagagcaaaa
     5581 caattatctc caatccctag aagtgcaagt catgctagac actcttcgtg tcattcacaa
     5641 tcccctgcaa gactacgcct tggttgccct tatgaagtct ccaatgtttg gttttgatga
     5701 ggatgagcta gcacgtttgt cccttcagaa agcagaggat aaagtccacg aaaatctcta
     5761 tgagaaactg gtcaatgcac aaaaaatggc aagtagtcaa aaaggcttga ttcacacagc
     5821 tctagctgaa aaactaaagc aattcatgga tatcctagct tcttggcgct tgtatgccaa
     5881 aacccactct ctctatgact tgatttggaa gatttacaac gaccgttttt attatgacta
     5941 tgttggggct ttgccgaatg gtcctgctag gcaggccaat ctctatgccc tagcactgcg
     6001 tgctgatcaa tttgaaaaga gcaatttcaa aggtttgtcg cgttttattc gtatgattga
     6061 ccaagtctta gaagcccagc acgatttggc aagcgtggcc gtcgcaccgc caaaagatgc
     6121 agtagagctc atgaccatcc acaagagtaa agggctggag tttccttacg tctttatcct
     6181 caatatggat caagatttca acaagcaaga ctctatgtca gaagtcattc tcagtcgtca
     6241 gaatggtctt ggtgtcaaat atattgccaa gatggagaca ggggcagtag aagaccacta
     6301 tcctaaaacc atcaaactct ccattcctag tctgacctat aggcagaacg aagaggaatt
     6361 acagctagca agctattctg agcagatgcg tttgctgtat gttgctatga cgcgggctga
     6421 gaaaaagctc tatcttgtcg gcaagggttc tcgtgaaaag ctggaagtca agcaataccc
     6481 agcagccaaa aatgggaaac taaatagcaa tactagactg caagcacgga atttccaaga
     6541 ttggctttgg gctatcagta aagtgtttac taaggacaag ctcaacttta gttatcgttt
     6601 tattggcgaa gatcagttga ccagagaagc tatcggagag ttggaaacca agagtcctct
     6661 ccaagatagc tcccaagcag acaatcgtca gtcagatacc atcaaagaag ctctggaaat
     6721 gctgaaggag gtggaagttt ataatactct tcaccgcgca gctattgaac ttcctagtgt
     6781 tcaaacccca agtcaaatca agaaattcta cgaaccagtt atggatatgg aaggtgtcga
     6841 gattgctggt caaggtcagt cagtaggcaa gaaaatcagc ttcgatttgc cagatttttc
     6901 aaccaaagaa aaggtaactg gagctgagat tggtagtgct actcacgaac tcatgcagag
     6961 aattgacctc agccagcaac taacccttgc tagcctaaca gaaacactca aacaagttca
     7021 aactagccaa gctgtcagag acaagatcaa tcttgataaa attcttgctt tctttgacac
     7081 agtactcggt caggaaattc ttgctaatac cgaccatctc tatcgcgagc aacctttctc
     7141 catgctcaaa cgagaccaaa agagtcagga agactttgtt gtccgtggta tccttgatgg
     7201 ctatctgctt tacgaaaaca aaattgttct gttcgactac aagacagacc gctatgatga
     7261 accaagtcaa ctcgtagacc gctatcgtgg tcagttagct ctatacgaag aggctttatc
     7321 acgagcctat tcgattgaaa atattgaaaa atacttgatt ttactcggta aagacgaggt
     7381 tcaagttgta aaagtataac ttatactctt tgaaaatctc ttcaaaccac gtcagcttcg
     7441 ccttgctgta tatatggtta ctgacttcgt cagttctatc cacaacctca aaacagtgtt
     7501 ttgagctgac ttcgtcagtt ctatccacaa cctcaaaaca gtgttttgag ctgacttcgt
     7561 cagttctatc tacaacctca aaacagtgtt ttgagctgat tgcggctagc tttctagttt
     7621 gctctttgat ttttattgag tattagaaag gagacctcat gacacttcca gttagaaaat
     7681 ccctgcacga tgcagtttta caggcttcga aagcggatac ttgggaacaa gctaccaagg
     7741 aatggaatga agtttccttg atttttaacg gtatcggccg tagcaattgt gtctgtggaa
     7801 atgccatcaa atacgcttat gaactcttta acggtgtcac aggccaacgc ctctttccaa
     7861 ttggtagcga ctgtgttcgt cattttcatc gattgtccct tgaccagcaa ttggaagagg
     7921 aagaaaaact gctcagaaag gttgaaaatc taaccagaaa agctcagaaa aaggaaaaaa
     7981 tcaaggtcaa taaaagtgac tttgacgagc gacttctaaa atggctctgg gaaaaaggtg
     8041 ttttcaaacc caatcgtggc aatcaatttg cgcctgagag agactaccag cttttcctag
     8101 aagtctttca gggaggaagt tggaccaagg cggaaccaaa gaaaaaggct cggatggaag
     8161 aagtccttga aaagtgtatc aaaccctttt tacttgggaa atcggatgac caactctacc
     8221 ttgtcaagct aggcaaggag aaaattgact acgaacaaga actccgtatc caggcagaga
     8281 aagaacgcaa gaagagggat aaaatcgcca agcaatatgc agacaatctg gttctagcca
     8341 tgggacccgc agagcgtgct tatcaagatt actttggctt tacagagacc ctgactcaag
     8401 aagaacgcaa gtgggaaaaa attctttttg gtaaaaatag agcagaacgg gctatcaagg
     8461 ccaaacaata ccaaaaagag ctggaaaaag accaacgaat tgccagtcag gatccaattg
     8521 aaagaaaaca gaagcagact tggctcctca attcctattt tcgtgagctt cctgaagaaa
     8581 aagctcgatt ttctaggctc ttattagaat atcgaaaaag tggtgaagta cccttttcaa
     8641 ctgaatacct gtccgatcat ctcatcgact ttttctataa aatgaaagcc tttgaatttg
     8701 aaatcgcacc agaacaagtc cgagattttc taaaaaaaag cctgcaggaa gaccatcgct
     8761 catcagcaca aggaagctgg atagaaggaa ttctcctcaa ctgcctcaaa ccatttttag
     8821 aacgattagt gatatgagta acacctaccg tgtaaatttc atggtaggtg ttattgtacc
     8881 cccaaataat ataaaacaaa gtatgaaagc gcatctcaat tttaagactt ttttgtaaat
     8941 taagagtata attaaggtat actg
//
LOCUS       DAVLVF010000077         8603 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVLVF010000077 DAVLVF010000000
VERSION     DAVLVF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.077"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(219..722)
                     /locus_tag="VYN66_001640"
     CDS             complement(219..722)
                     /locus_tag="VYN66_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495631.1"
                     /translation="MKLLDCILDYQERFNRKACQVSTNYKYLETFKVNFCLTDLHHLF
                     GLHKITRDYASQTKPAIQAGVFILEDFRNILLYNDVIERISLYEFIGDIFYFKITSCC
                     IVAKDLSKNTMKLDVIFFEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTD
                     VKEMKWL"
     gene            complement(1857..2585)
                     /locus_tag="VYN66_001641"
     CDS             complement(1857..2585)
                     /locus_tag="VYN66_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495632.1"
                     /translation="MNNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFATKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNRVLALI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIVYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEFELFKNSK"
     gene            complement(2582..2914)
                     /locus_tag="VYN66_001642"
     CDS             complement(2582..2914)
                     /locus_tag="VYN66_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV1495633.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICVALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGKIAYCIGYGALNRGCDISGNPYPWD
                     GFISATVR"
     gene            complement(2970..3872)
                     /locus_tag="VYN66_001643"
     CDS             complement(2970..3872)
                     /locus_tag="VYN66_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495634.1"
                     /translation="MMRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFVIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTNTLKDKIQKSDLSYSDLMSLQLYDAMNQSTFLTDSELFEDILSKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            complement(3878..4195)
                     /locus_tag="VYN66_001644"
     CDS             complement(3878..4195)
                     /locus_tag="VYN66_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495635.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGDWNTFKNNIKGLARASSGFRF
                     AARETGLWAAIWSTRVYSYTYVVSAFAPILGSVAAVNAALAVIGGISVAGITYALWTR
                     KFY"
     gene            complement(4340..5692)
                     /locus_tag="VYN66_001645"
     CDS             complement(4340..5692)
                     /locus_tag="VYN66_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEV1495636.1"
                     /translation="MKLYNKSELRYSRIFFDKRPPAFAFILIISTAIILSGALVGAAY
                     IPKNYIVKANGNSVITGTEFLSAIGSGKVVTLHKSEGDMVNAGDVIISLSSGQEGLQA
                     SSLNKQLEKLRAKEAIFQKFEQSLNEKYNHLSNSGEEQEYYGKVEYYLSQLNSENYNN
                     GTQYSKIQDEYTKLNKITAERNQLDADLQALQNELLQLQQQGDSPSLSDTTSADNKAK
                     LETKISEITTKIEALKTNITSKNSEIDSQQSNIKDMNRTYNDPTSQAYNIYAQLVSEL
                     GTARSNNNKSITELEANLGVATGQDKAHSILAPNEGTLHYLLPLKQGMSIQQGQTIAE
                     VSGKEKGYYVEAFVLASDISRVSKGAKADVAITGVNSQKYGTLKGQVRQIDSGTISQE
                     TKEGNISLYKVMIELETLTLKHGSETVVLQKDMPVEVRIVYDKETYLDWILEMLSFKQ
                     "
     gene            complement(5689..6156)
                     /locus_tag="VYN66_001646"
     CDS             complement(5689..6156)
                     /locus_tag="VYN66_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1495637.1"
                     /translation="MAKLEVKDNKKLVLKSVICKKLHDTKVEDVDQEINKFHQHLQLL
                     KAQIFGPLIVKSCGTTIHDDGLITTDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVR
                     FEDSPEYLQYAYSKLDLYVYENDIQTDGIVYTVYVNSSPEKMVVDIFRPIVSL"
     gene            complement(6149..8368)
                     /locus_tag="VYN66_001647"
     CDS             complement(6149..8368)
                     /locus_tag="VYN66_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEV1495638.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     RNSKFYVADPASGIRKMSSDELGEIYQGITLFMVPNSDFERGKLKGKGLLDLFGRLIF
                     NQKGLISTVILASFVLSIIGILSSLFSKVIMDEVIPYALKNSLYMFLIVFGIVSFLQT
                     LLSAFRQHVLLFLSRKIDIPVLMGYYDHIIHLPYSFFGSRRVGDVLTRFQDAMTIKNV
                     FTSVSISLVMDITLSVISAVVLWTINQSLFLILVFMVIVNIILIYCFKKPYKKINHEQ
                     MEANGLLNSQLIESIRNIDTIKSQHDEEQRLNKIEEKFVHTLEIGYKEGVLQNIQSTI
                     SSMTSTMGGLLFMGVGALFIIDGKMTIGDLLVFQTLSQYFTEPIQNLVGLQLTFQEVQ
                     VAVSRLQELMEVDREDIALDYSIRDFTLCDDIEFKDVTFAYGSRPPVIKDFNLRIKQG
                     EKIAFVGESGAGKSTLVRLLLRFINPSEGKIRIGENDLSDLDYGKLRKKISYIPQTIE
                     LFTGTIIDNLKIGNPSVTYEDMVRVCRIVGIHDTIQRLQNRYGSFVEEGGQNFSGGEK
                     QRLAIARALLSKADLYIFDEATSNLDSFSEQIIQDLIFNKIMDKTTIVVAHRLSTILR
                     CDKICFLENGTIMEYGTHEELMAKNGKYARMVGLQSVQVNQQIQSQAVLDTEEVTYG"
BASE COUNT         2913 a         1642 c         1162 g         2886 t
ORIGIN      
        1 atatttgtca aatgttttat attacatttt tattacaaat ttctatatca taagcttcta
       61 ctccaaaaag ataaagacac tacttttgcc cttttcgatt ctctttttaa acatagcatc
      121 atcttccttc attcctataa aaacatttcc gatgagagag cttgtttttt aatttcgtta
      181 aacaacaaaa aacgcaagct taagcctgcg gacaatcttt atagccattt catttctttc
      241 acatctgttt tacgaacttt agcatatttc ttagcgcttg taaaatgtag agtaactggt
      301 ttaaatactc cgcttttgtc tcttcgtaaa cctaaaactg cggatctttt atttttatcc
      361 tcaaaaaata tgacgtccaa tttcatagta tttttagata aatccttagc tacgatgcaa
      421 caacttgtta ttttaaaata gaaaatatca cctataaact catataaaga tatcctttcg
      481 attacatcat tgtacaatag gatatttcta aaatcttcta aaataaaaac accagcttga
      541 atagcaggtt ttgtttgact agcataatct cgtgtgattt tgtgtaagcc aaataaatga
      601 tgtaaatcag tcaggcaaaa attcactttg aaagtctcta aatacttata attcgttgat
      661 acttgacatg cttttctatt gaatctttct tgataatcca aaatacaatc aagtaatttc
      721 aacgacaaac tccatatttt ctcaaaataa aaaagcacgg ggcagttctc actactgccc
      781 aaagtggttg attagagtcg gcttctctaa ctccctatgt tgtgcttatt aggcttatcg
      841 caagggaaca gtttttagct ctgccaagcc acatagtgtg cttactacta tgtgtccatg
      901 tcaccatgaa ttgccgaacg gtttaacgag aaagtatagt agattgaaac aagatgtgaa
      961 caaatcgatt aggaaagtca aattaatttc tagaaatatt ttaacagtca taacggacta
     1021 ttccagattc aatctactat attactttta ggtttatccc aactcaatta tgacatcttt
     1081 ttttcaaaag tcaatatatc tcactctttt caacgacaag aaagaggctg ataatcgacc
     1141 aacctctgtt tttagataca gttcaagtcc aaactattct ttatagaaaa gtccgataaa
     1201 ttcgagcaat agttaacata cttacaaata taactcccaa aatagataac actattttca
     1261 caaaactcag ttccgaacct atgacatata tatgaaaaag aggggctagt cctacacata
     1321 tatacaataa gtagccaaca agttttatct caccaattcc tgcaaaaaaa tgtttcatgt
     1381 aacgattctc catttactct attaaatcaa ttttatttta gactatcata acacaatcac
     1441 ctcttcaata aaaaccattg gcgaaatact attctgaagc attgatagct cagatttctc
     1501 agaatagagc tgtcctaact atcgagcagc tcaaatacat atatatgagc aattctaagt
     1561 ttagaattgc tataaaataa atgtttccag gctattctaa gtctttgact gcttaaaatt
     1621 ttacgttctg aaggattcta aagctagaac agctcaaaat cacgagaact aaggtattcg
     1681 aaacttcaga gtaatacaat ccccatagga attctccatt cttattaagc atgattaaaa
     1741 tgtaatccta acaagtgatc ctcgactgtg tttgtagcac ttagttacct agataagact
     1801 atttcccttt atcatatctg attttaagcc gttctaagat ttaacctatg tttctattac
     1861 ttgctatttt taaataattc gaattccttt tctttgataa atggaactga aaaattagaa
     1921 aaaagaagta aaaccgaaaa caaatagcct gctttttgaa tcactaaaag atatcctaaa
     1981 aataagtaaa ctataacatc caatgcgaaa agaaaaaata ttctcctctt cattaaagta
     2041 cttgtgtcaa ataatctgat gaataaaaat tttatcgtag tatctacaca aatttttatc
     2101 aaaaagataa gagccaatac acgattatca actgtaccgt aaagaataga tggaaatata
     2161 gttaatataa aactagaaat ctctaaatat tttatccaag gagattgaga caccttgctt
     2221 ctaaactgac taattttctt atattcgtta gtcaatttag tagcaaaatc ttccccaatt
     2281 aaatgaaaga ttgctaactc taggataaaa acacttatta atacggtata agtcaaaaaa
     2341 ctttctggga aaaacttatt gattaaaatg gggaaactca ttaacagaat cgaatttatg
     2401 gctagacaga tagatactat tttatttttc acagaatata gaggtagtga tactattgag
     2461 tagataaaag ctaaaaaaat aagcaaaaaa tactccgtgt catttataat cgccctgaat
     2521 gataaaaata tatacatcca acatacgata atatgtattt ttcgaatctt atctacatta
     2581 ttcatcttac tgtcgcagat ataaatccat cccaaggata agggttcccg ctaatatcac
     2641 aacctctatt aagagcacca tatccaatac agtaagctat ttttcctgct gctgcaccaa
     2701 taagccagcc tccaagacct ccaaccttaa tatagtttat tagttttttg gttaaaactg
     2761 cagctccagc taatgctata aaagttcctg aactcatagc taaagctacg caaattcctt
     2821 gacaagcacg cttgcttaaa taagctcccc catcaagata catcatttct tcttcatcaa
     2881 tcacaacata attatttggt aatacaagtt ccatttgttt tctcctcctt agttgaaatt
     2941 gtttcatttg ttttacaaat aggacgaact taaactatct ttttattact cagtaataat
     3001 ttccgagatt tcattttatg atactctcta ttcatacaga aatcaaaatt ttgccttaac
     3061 caacttgtat aatagaaaaa atctaattga tttattctga tgagctgtat tctaatttta
     3121 tcaacctttt cttgccagtt gttattgtta ctaatagaaa tctcatacag cagtaactcg
     3181 acaagacctg tacacagaag ggtgttatga gaatcttcaa ttttactcag aatatcctca
     3241 aaaagttcac tatccgttaa aaaggtggat tgattcatcg catcatacag ctgaagagac
     3301 attaagtcag aataactgag atctgatttt tgtattttat cctttaacgt atttgtcaaa
     3361 tcactttcaa acgaatcttc tttttcccat ttattttgat tgaaaagaaa ttccgtctta
     3421 ttaaaaacga gatgattgat atccattata gtccaaaaaa atattgtagt aagcataatg
     3481 atgacaaaag gaatttttaa aataaatact aggataatag caatttcaag aataaaactc
     3541 catataatcc tagatcttaa atcatcaggc gacaaacttt tacgatggaa tgaaaaatag
     3601 ggcgagaacc tcacatttac tttcttcctg cctataaaaa taaaaggaaa aatcgtaata
     3661 agagcttctt tcttgtacaa atctttcaat gcgtatagta tgtctaaaac tatttttgat
     3721 aaaatcaaac tacagaaagc aagactcata ctaatcacaa aatcaagggg agattctaga
     3781 ttcaatagta agttattcac taggacatgc aactctagcc acaagacaaa aagagggagc
     3841 attagtaaga aaacaattag tttacgcatt ataacaatta gtaaaattta cgcgtccata
     3901 aagcatacgt aattcctgca actgaaatac ctccaattac agccaatgcc gcattaacag
     3961 ctgctactga ccctagtatt ggagcgaaag cagaaacaac ataagtataa gaatacactc
     4021 ttgtagacca tattgcagcc cataaaccag tttctcttgc ggcaaaccta aatcctgagg
     4081 aagctctcgc taaacctttt atattatttt taaatgtgtt ccagtctccc ccatcaaggt
     4141 acatcatctc ttcttcatca atcacaacat aattatttgg taatacaagt tccatttgtt
     4201 ttctcctttt tattaagcat ggttaggatg gtttcctaac aagtaatcgt cgtctgtgtt
     4261 tctatcactt agctacctag ataagatatc ctttctctac cttatttgtt tttataggtt
     4321 acctaaggtt taaaaccaat tattgcttga aacttaacat ttctaaaatc caatcaagat
     4381 aggtttcttt atcatagaca atccgcactt caactggcat atccttttgg aggacgactg
     4441 tctcacttcc atgttttaga gttaaggttt ctaattctat catgacttta taaagactaa
     4501 tattgccttc tttcgtttct tgggaaattg ttcctgaatc aatctgtctg acttgtccct
     4561 ttagtgttcc atatttttga ctatttacac cagtaatagc aacatcagct tttgctcctt
     4621 tcgaaacacg agaaatatca ctcgcaagta caaaagcttc tacatagtaa cctttttctt
     4681 tccctgaaac ttctgctatc gtttgcccct gctgaataga cattccttgt ttcaaaggta
     4741 acagataatg cagagtacct tcatttggcg ctaatatact atgagcttta tcttgacctg
     4801 ttgccactcc aagattagcc tcaagctctg taatactttt attgttgttt gaacgagcag
     4861 tacctaactc actaactaat tgagcataaa tattataagc ctgagaagtt ggatcattat
     4921 aggtacggtt catatcctta atattgcttt gttgactatc aatctcgcta ttcttagaag
     4981 taatatttgt ttttaatgct tcgatttttg ttgttatttc tgatatctta gtttctaact
     5041 tagctttgtt atcagctgat gtcgtatctg atagagaagg tgagtctcct tgctgttgaa
     5101 gttgaagcaa ttcattttgt agagcttgca agtcggcatc taactgattt ctttcagctg
     5161 ttattttatt caacttcgta tattcatcct gaatctttga atactgggta ccattattat
     5221 aattttccga atttaactga gataggtagt attcaacttt cccataatat tcctgttctt
     5281 caccagaatt agataaatga ttgtatttct catttaatga ttgttcaaat ttttgaaaga
     5341 tagcttcttt tgcacgtaac ttctccaatt gtttatttaa agagctcgct tgtaaacctt
     5401 cttgcccact tgataacgaa ataatgacat ctccagcatt taccatatct ccttctgact
     5461 tatgtaaagt aactaccttc cctgaaccaa ttgctgatag gaactctgta cctgttataa
     5521 ctgaatttcc attcgctttt acaatatagt ttttgggtat ataagctgcg ccaaccaatg
     5581 caccgcttaa gataatagca gttgaaataa tgagaataaa cgcaaaagct ggtggtctct
     5641 tatcaaagaa aatacgagaa taacgtaatt ctgatttatt atataatttc ataggcttac
     5701 aattggtcta aaaatatcta ctaccatttt ttcaggagaa gaattaacat aaactgtata
     5761 gacaatccca tccgtttgaa tatcattttc atagacatat agatccaatt tagaatacgc
     5821 atactgtaga tactctggac tgtcttcaaa acgaacatat aaacaatatg gaacagagat
     5881 agaatcctgt acatcataaa tgttactgta ctgttgagca ttatgagctt gaatataaaa
     5941 ctcaaaatca gtcgttatta atccatcatc atgaatagta gtaccacaac tttttacaat
     6001 taatggacca aaaatttgtg cttttaacaa ctgcaaatgt tgatgaaatt tattaatttc
     6061 ctgatctaca tcttctactt ttgtatcatg taacttttta cagataactg actttagtac
     6121 cagtttttta ttatctttta cctctaactt agccataagt aacctcctct gtatctaaca
     6181 cagcctgtga ctgaatttgt tgattcactt gaacgctctg caaaccaacc attctagcat
     6241 actttccatt tttcgccatt agttcttcat gagtaccata ttccataata gtcccatttt
     6301 ctaaaaagca tatcttatca catcgaagaa ttgtagacag cctgtgggct actacaattg
     6361 ttgtcttatc cattatttta ttaaagatta aatcctgaat aatctgttca ctaaatgaat
     6421 ctaagttaga ggttgcctca tcaaatatat acaaatcagc tttactcagt agtgctcttg
     6481 caatagccaa tcgttgtttc tctccacccg agaaattttg accgccttct tctacaaagg
     6541 agccataacg attttgaaga cgttgaatcg tatcatgaat accaacaata cgacaaactc
     6601 tcaccatatc ctcatatgta acagaaggat taccaatttt tagattatca ataattgtcc
     6661 ctgtaaagag ttctatggtc tgaggaatgt aggaaatttt cttacgtagt ttaccatagt
     6721 caagatctga taagtcattt tctcctatac gaattttccc ctcactcgga tttataaatc
     6781 ttaataacaa acgaactagc gtactttttc ctgctccgct ttctccaaca aaagctattt
     6841 tttctccttg tttaattcgc aaattaaagt ccttgataac aggagggcgt gaaccatagg
     6901 caaatgttac atctttaaat tcaatgtcat cacataaggt gaagtctcta atactataat
     6961 ccaaggcaat gtcttcacga tctacttcca tcaattcttg aagccgacta accgcaacct
     7021 gtacctcttg aaaggttaac tgcaagccaa ctaaattctg aataggttct gtaaaatatt
     7081 gacttaatgt ctgaaaaacc aagaggtccc caatagtcat ttttccatca atgataaata
     7141 aggcgcccac tcccataaat aataacccac ccatggttga ggtcattgaa gatattgtag
     7201 attgaatatt ctgcaaaaca ccctctttat aaccaatttc taaagtatga acaaattttt
     7261 cttcaatttt gtttaaacgt tgttcctcat catgctgaga cttaatggta tcaatgttac
     7321 gaatagattc aattaactgt gaattaagaa gtccatttgc ttccatttgt tcatgattaa
     7381 tttttttata aggcttttta aaacagtata taagaatgat gttaacaata accataaaaa
     7441 ctaatatcag aaataaagat tgattaatag tccaaaggac aaccgcacta ataactgaca
     7501 aggtgatatc catgactaat gaaatagaga ctgacgtaaa cacattcttt atcgtcatcg
     7561 catcttgaaa tcttgttaaa acatcaccaa ctctacgact tccaaaaaaa ctataaggaa
     7621 gatgaataat atggtcataa tatcccatta aaaccggtat atcaattttt cgagatagaa
     7681 acaaaagaac gtgctgacga aatgcagata gaagagtctg taagaacgac actattccaa
     7741 atactatcaa aaacatatac agactatttt ttaaagcata tggaatcacc tcgtccatga
     7801 taacttttga aaagagactc gataaaatac caatgatact taaaacaaag gaagctaata
     7861 tgactgtact aatcaatcct ttttgattaa aaattaacct tccaaataaa tcaagaagtc
     7921 cttttccctt tagtttccct ctttcaaaat ccgaatttgg aaccataaaa agagtaatac
     7981 cttgataaat ttctcccagt tcgtcacttg acatcttgcg tatgccacta gcagggtctg
     8041 ctacgtagaa cttactattc cttttgatac tatgtagtac cacaaaatga ttttgaccta
     8101 aatcattctt aacttgaaga atcgcaggaa atgttaattt tggagtcaaa tcttccagtg
     8161 ctactcgaac agcttttact gtaaaattca acttattcag acctgaaaca ataccactga
     8221 cagtcgttcc atacatgtct gtaccaataa tttccctaat cttcatgatt gatagttcct
     8281 ttttgtaaga taaaagaact gttgaaacaa ctgccgcagc acaatctgac tggtcatgtt
     8341 gcatcgtaca aggatatttt tttaacatag aaatactcaa ttctttagaa ttaatctaac
     8401 ttttctttga tgtcacccaa acctcaatca ctgcccagta gttcttgaat taatttattt
     8461 tcaaccgtat cattctttgt tcttgatatc tttccaagca ttgtagtaca gcagattttt
     8521 gcccaaggac tgagcctcct ttaacaactc aaactccctt acattatatc ggccagcccc
     8581 atggaaagat tatagccaga cta
//
LOCUS       DAVLVF010000078         8574 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVLVF010000078 DAVLVF010000000
VERSION     DAVLVF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.078"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            130..528
                     /locus_tag="VYN66_001648"
     CDS             130..528
                     /locus_tag="VYN66_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1495639.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            973..1980
                     /locus_tag="VYN66_001649"
     CDS             973..1980
                     /locus_tag="VYN66_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1495640.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSKMEIIFIKRF
                     Q"
     gene            2055..2255
                     /locus_tag="VYN66_001650"
     CDS             2055..2255
                     /locus_tag="VYN66_001650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV1495641.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            2290..3006
                     /gene="atpB"
                     /locus_tag="VYN66_001651"
     CDS             2290..3006
                     /gene="atpB"
                     /locus_tag="VYN66_001651"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV1495642.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            3020..3514
                     /gene="atpF"
                     /locus_tag="VYN66_001652"
     CDS             3020..3514
                     /gene="atpF"
                     /locus_tag="VYN66_001652"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV1495643.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            3514..4050
                     /locus_tag="VYN66_001653"
     CDS             3514..4050
                     /locus_tag="VYN66_001653"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV1495644.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            4065..5570
                     /locus_tag="VYN66_001654"
     CDS             4065..5570
                     /locus_tag="VYN66_001654"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV1495645.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            5586..6464
                     /locus_tag="VYN66_001655"
     CDS             5586..6464
                     /locus_tag="VYN66_001655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV1495646.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            6550..7956
                     /gene="atpD"
                     /locus_tag="VYN66_001656"
     CDS             6550..7956
                     /gene="atpD"
                     /locus_tag="VYN66_001656"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV1495647.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            7967..8386
                     /locus_tag="VYN66_001657"
     CDS             7967..8386
                     /locus_tag="VYN66_001657"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV1495648.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIYQERRAKIALQRAINRINVGNRL"
BASE COUNT         2655 a         1572 c         1856 g         2491 t
ORIGIN      
        1 attgagtatt agatgtatag tgcattgaag ttaaaatagt acactttgat ttctaagaca
       61 ttgttagaaa ttagattaaa ttccttaatc gatttgcgca tgttttattt catttcacta
      121 tactttagat tggaaaataa ggaatcacat aaatcgcaag aaatagggaa tctgattcgt
      181 gtttataacc gttcaaaaag agaagaggct gaaagtgagc cacttaatct ttatgtcgaa
      241 gatgaaaagg gcaatctcct ggcaggtttg atagcagaga cttttggaaa ttggctagaa
      301 atcgagtatt tgtttgtaaa agaggaactg cgagggcaag gaatcggttc aaaactattg
      361 caacaggcag aaagtgaagc taagaatcga aattgttgtt ttgcctttgt caatacttac
      421 cagttccagg caccggactt ttatcagaaa catggctaca aggaagtttt ttctttgcaa
      481 gactatctct acattaggca aagatattat taccaaaaga atctgtaagg tgagtatgga
      541 ggtataatac aaagagaggc tcgcacataa gtatggaggt ggactgatgt atatgagtaa
      601 ctatcgataa ataccagtta taacctagaa attgtgtctt tctagacgat attgaggaca
      661 atataatcgt agccccaaaa ttaggaatca agacttatca ggttaagaaa agaagtgatg
      721 ttgttgatat tttaaacaat ttatttaaac taaaaactct ctatctatta atccgagatt
      781 cccctctcgg attttgtgtt gctttcatta aatttacagg taagacttgc tagcttcagt
      841 aaatatacac aagtttttca gaatagagtg aaatccaaaa gaaaatggaa ataatgaaag
      901 ctcttgtagg cataagaaaa cacctctgtg ttatacttgt tgttcaacca caaacacaag
      961 aaaggcacag agatgcaaga caattatact acaaaagcca aacatttgac aatcgatagc
     1021 cgtcgcttaa tcgaaagatg gaaaaaagaa gggaaatcaa atagagaaat tgcctctctg
     1081 cttggaaaag ttcctcaaac tatccacact gaaatcaagc gtgggacagt ccgacaatgt
     1141 cttggaaaag ggcgcttcaa agaggtttat tctgccgact atgctcaaca gtcttatgaa
     1201 aataatcgca agcgctcggt caagaaatca agcttgacca aggaactaaa ggaaaagatt
     1261 ctccactatc ataaccaaaa attttcgcct gaaatgatgg tgatggctaa aggggttaac
     1321 gtgggaattt caaccattta ctattggatt catcatggaa aattggggtt aagcaaacag
     1381 gatctgcttt accctagaaa aggaaaagcg cttaagaaac aggctagcac caactttaaa
     1441 cctgctggtc aatccatcga acagcggtct gaagctatca atcttcgctt ggagaatggg
     1501 cattatgaga ttgatacggt tctacttacg agagcgaaaa actactgctt gcttgtcttg
     1561 acggatcgaa agagtagaca tcagatcatc cgattgattc caaataaaag tgctgaggtg
     1621 gtcaatcagg ctctaaaact catcttaaaa caacacaaga ttctttccat cacggcagat
     1681 aatggaacgg aattcaatcg cttgtttgat gtattttctg aggagcacat ctattatgcg
     1741 cacccctatg cctcttggga aaggggaact aatgagaatc acaacaggct cattcgtaga
     1801 tggttaccta agggaaccaa gaaaatgact cccaaagaag tcgcattcat cgaaaagtgg
     1861 attaacaact atcctaaaaa atgcttggac tacaagtcac ccagagaaga cttctggatg
     1921 gctaacttga acttgaaatt tagcaaaatg gaaataattt ttattaaacg cttccaatag
     1981 cggttaaaag ttgacaaatt taaattttag gactacacta agtaagtaaa ttttatttta
     2041 aaggagaatt tgttatgaat ttaacatttt taggcttatg tattgcctgt atgggcgtat
     2101 ctgtcggtga aggtttattg atgaatggac tgtttaaatc agtagcacgc caaccagata
     2161 tgctttctga gtttcgtagt ttgatgtttt taggtgttgc ctttattgaa ggaactttct
     2221 ttgtaactct tgtcttctca tttattatca aataaataca tggaacgaga agaaaaggga
     2281 ggattttaga tggaagaaag tattaatcca atcatctcta ttggtcctgt tatcttcaat
     2341 ctgactatgt tagccatgac tttgttgatt gtgggagtta tttttgtctt tatttattgg
     2401 gcaagccgca atatgacctt gaaacccaaa ggaaagcaaa atgtacttga gtatgtctat
     2461 gactttgtta ttggatttac agaacctaac attggttcgc gctacatgaa agattactca
     2521 ctctttttcc tttgtttatt ccttttcatg gtgattgcca ataaccttgg cttaatgaca
     2581 aagcttcaaa cgatcgatgg gactaactgg tggagttcgc caaccgctaa tttacagtat
     2641 gacttaacct tatcttttct tgtcattttg ttgacacata tagaaagcgt tcgtcgtcgt
     2701 ggatttaaaa aaagtataaa atcttttatg agtcctgttt ttgtcatacc gatgaatatc
     2761 ttggaagaat ttacaaactt cttatctttg gctttgcgga tttttgggaa tatctttgca
     2821 ggagaggtca tgacgagttt gttacttctt ctttcccacc aagctattta ttggtatcca
     2881 gtagcctttg gagctaattt ggcttggact gcattttctg tctttatttc ctgcatccaa
     2941 gcttatgttt ttactctttt gacatctgtg tatttaggga ataagattaa tattgaagag
     3001 gaatagaaag gagtaactga tgcacgtaac agtaggtgaa ttaattggta attttatttt
     3061 aatcactggc tcttttattc ttttgctagt cttgattaaa aaatttgcat ggtctaatat
     3121 tacaggcatt ttcgaagaaa gagctgaaaa aattgcttca gatattgaca gagctgaaga
     3181 agcccgtcaa aaagcagaag tattggctca aaaacgcgaa gatgaattgg ctggtagccg
     3241 taaagaagct aagacaatca ttgaaaatgc aaaggaaaca gctgagcaaa gtaaggctaa
     3301 tatcttagca gatgctaaac tagaagcagg acacttaaaa gaaaaagcca atcaagaaat
     3361 tgctcaaaat aaagtagaag ctttacagag tgttaagggt gaggtcgcag atttgaccat
     3421 cagcttagct ggtaaaatca tctcacaaaa ccttgacagt catgcccata aagcactcat
     3481 tgatcagtat atcgatcagc taggagaagc ttaatggaca agaaaacagt aaaggtaatt
     3541 gaaaaataca gcatgccttt tgtccaattg gtacttgaaa aaggagaaga agaccgtatc
     3601 ttttcagact tgactcaaat caagcaagtt gttgaaaaaa caggtctgcc ttctttttta
     3661 aaacaagtgg cagtagacga gtcggataag gaaaaaacaa ttgctttttt ccaagattct
     3721 gtgtcgcctt tattacaaaa ctttatccag gttctggcct acaatcacag agcaaatctt
     3781 ttttatgatg tgcttgtaga ttgcttgaac cgacttgaaa aagaaacaaa tcgatttgaa
     3841 gtgacgatta cgtctgctca tcctctaact gatgaacaga agactcgttt gctccctttg
     3901 attgagaaaa aaatgtctct gaaagtaagg agtgtaaaag aacaaatcga tgaaagtctc
     3961 attggtggtt ttgtcatttt tgccaatcac aagacaattg atgtgagtat taaacaacaa
     4021 cttaaagttg ttaaagaaaa tttgaaatag aaagtggtgt tcttttggca attaacgcac
     4081 aagaaatcag cgctttaatt aagcaacaaa ttgaaaattt caaacccaat tttgatgtga
     4141 ctgaaacagg tgttgtaacc tatatcgggg acggtatcgc gcgtgctcat ggccttgaaa
     4201 atgtcatgag tggagagttg ttgaattttg aaaacggctc ttatggtatg gctcaaaact
     4261 tggagtcaac agacgttggt attatcatcc taggtgactt tacagatatc cgtgaaggcg
     4321 atacaatccg ccgtacaggg aaaatcatgg aagtccctgt aggtgaaagt ctgattggtc
     4381 gtgttgtgga tccgcttggt cgtccagttg acggtcttgg agaaatccac actgataaaa
     4441 ctcgtccagt agaagcacca gctcctggtg ttatgcaacg taagtctgtt tcagaaccat
     4501 tgcaaactgg tttgaaagct attgacgccc ttgtaccgat tggtcgtggt caacgtgagt
     4561 tgattatcgg tgaccgtcag acagggaaaa caaccattgc gattgataca atcttgaacc
     4621 aaaaagatca agatatgatc tgtatctacg tcgcgattgg acaaaaagaa tcaacagttc
     4681 gtacgcaagt agaaacactt cgtcagtacg gtgccttgga ctacacaatc gttgtgacag
     4741 cctctgcttc acaaccatct ccattgctct tcctagctcc ttatactggg gttgctatgg
     4801 cggaagaatt tatgtatcaa ggtaagcatg ttttgattgt atacgatgat ctatcaaaac
     4861 aagcggtagc ttatcgtgaa ctatcgctct tgcttcgtcg tcctccaggt cgtgaagcct
     4921 tcccagggga tgttttctat ctccacagcc gtttgcttga gcgctcagct aaagtttctg
     4981 atgaacttgg tggtggatca attacagccc taccatttat cgagacacaa gcaggagata
     5041 tctcagccta tatcgcaacc aacgtgattt ctatcactga tggacaaatc ttccttggcg
     5101 atggcctctt caatgcaggt attcgtccag ccatcgatgc gggttcatct gtatctcgtg
     5161 taggtggttc tgcacaaatc aaagccatga agaaggttgc tggtacactt cgtatcgacc
     5221 ttgcttcata ccgtgagttg gaagccttta ctaagtttgg ttctgacttg gacgcagcaa
     5281 cacaggctaa gttgaaccgt ggacgtcgta ccgttgaggt cttgaaacaa cctgttcaca
     5341 aaccattacc tgttgagaaa caagtaacca ttctttatgc tttgacacat ggtttcttgg
     5401 atactgttcc agtagatgat attgttcgtt tcgaggaaga gttccatgcc ttctttgatg
     5461 ctcaacatcc agagattttg gaaaccattc gtgatacaaa agacttgcca gaagaagcag
     5521 tcttggatgc tgcgattaca gagtttctca atcaatctag cttccaataa gaatagaggt
     5581 gtcagatggc agtgtctcta aatgatatta aaacaaaaat cgcctcaaca aaaaatacga
     5641 gtcaaatcac taatgccatg caaatggtat cggctgctaa gttaggtcgc tctgaagaag
     5701 ctgctcgcaa cttccaagtt tacgctcaga aagtgcgtaa acttttgaca gatatccttc
     5761 atggtaatgg agctggtgct tcaactaatc cgatgttgat tagccgttct gtgaagaaga
     5821 caggctatat cgttatcact tcagaccgcg gtttggttgg aggttataat tcctctattt
     5881 tgaaagctgt tatggagttg aaagaagaat accacccaga cggtaaaggt tttgaaatga
     5941 tctgtatcgg tgggatggga gctgatttct ttaaggctcg cggtattcaa ccactttatg
     6001 aattacgtgg cttggcagac caacctagct ttgatcaagt tcgtaagatt atttcaaaaa
     6061 ctgttgaaat gtaccaaaat gaactctttg atgagcttta tgtttgctac aaccaccatg
     6121 tcaatacgct aaccagtcaa atgcgtgtgg aacaaatgct tccgattgtt gacttggatc
     6181 caaatgaagc ggatgaagag tacagcttga cttttgaatt ggaaaccagc cgagaagaaa
     6241 ttctggagca gttgttgcct cagtttgcag aaagtatgat ttacggtgcc attatcgatg
     6301 ccaagacagc tgagaatgct gcgggtatga cagccatgca aacagcgaca gataatgcta
     6361 agaaagtcat caatgatttg acaattcagt ataaccgtgc cagacaggcg gcgattacac
     6421 aagaaattac agaaatcgta gcaggtgcta gtgccttaga ataggctcta gtccagctcg
     6481 tatgaaaatg aacttaggac ctagttgagc taggaaccga cagtatctta tatagaatag
     6541 gagaaggaga tgagttcagg taaaattgct caggttatcg gtcccgttgt agacgttttg
     6601 tttgcagcag gggaaaaact tcctgagatt aacaatgcac ttgtcgtcta caaaaatgac
     6661 gaaagaaaaa caaaaatcgt ccttgaagta gccttggagt taggagatgg tatggttcgt
     6721 actatcgcca tggaatcaac agatgggttg actcgtggaa tggaagtatt ggacacaggt
     6781 cgtccaatct ctgtaccagt aggtaaagaa actttgggac gtgtcttcaa cgttttggga
     6841 gataccattg atttggaagc tccttttaca gaagacgcag agcgtcagcc aattcataaa
     6901 aaagctccaa cttttgatga gttgtctacc tcttctgaaa tccttgaaac agggatcaag
     6961 gttattgacc ttcttgcccc ttaccttaaa ggtggtaaag ttggactttt cggtggtgcc
     7021 ggagttggta aaaccgtctt aatccaagaa ttgattcaca acattgccca agagcacggt
     7081 ggtatttcag tatttactgg tgttggggaa cgtactcgtg aggggaatga cctttactgg
     7141 gaaatgaaag aatcaggcgt tatcgagaaa acagccatgg tctttggtca gatgaatgag
     7201 ccaccaggag cacgtatgcg tgttgccctt actggtttga caatcgctga atacttccgt
     7261 gatgtggaag gccaagacgt gcttctcttt atcgataata tcttccgttt cactcaggct
     7321 ggttcagaag tatctgccct tttgggtcgt atgccatcag ccgttggtta ccaaccaaca
     7381 cttgctacgg aaatgggtca attgcaagaa cgtatcacat caaccaagaa gggttctgta
     7441 acctctatcc aggctatcta tgtgccagcg gatgactata ctgacccagc gccagcaaca
     7501 gccttcgctc acttggattc aacaacaaac ttggaacgta aattggtaca attgggtatc
     7561 tacccagccg ttgacccact tgcttcaagc tcacgtgcct tggcacctga aatcgttgga
     7621 gaagagcact atgcagttgc tgctgaagta aaacgtgtcc ttcaacgtta ccatgaattg
     7681 caagatatca ttgctatcct tggtatggat gagctttctg atgaagaaaa gaccttggtt
     7741 gctcgcgccc gtcgtatcca gttcttcttg tcacaaaact tcaacgttgc ggaacaattt
     7801 actggtcagc caggttctta tgttccagtt gctgaaactg tacgtggctt taaggaaatc
     7861 cttgatggta aatacgacca cttgccagaa gatgccttcc gtggtgtagg ttctatcgaa
     7921 gatgtgattg caaaagctga aaaaatggga ttttaagagg tgacctatgg ctcagttaac
     7981 tgtccagatc gtgacaccag atggtctcgt ctatgatcac catgccagct atgtatcggt
     8041 tcgaactctg gatggtgaga tggggatctt gccacgacat gaaaatatga ttgcggtttt
     8101 agcagttgat gaagtaaagg taaaacgtat cgatgataaa gatcacgtga actggattgc
     8161 agtaaacggt ggcgttattg aaattgccaa tgatatgatc acaatcgtcg ctgactctgc
     8221 agaacgtgct cgtgatatcg atattagccg tgcagaacgt gccaaacttc gtgcagaacg
     8281 tgcaattgaa gaagcacaag acaaacattt gatttaccaa gaacgtcgtg ctaagattgc
     8341 tttgcaacgt gccattaacc gtattaatgt cggaaataga ctataagaaa aaatgaactt
     8401 gaaaatacca agttcatttt ttaggctctt tgtcaactgt agtgggttga aaaaaagcta
     8461 agctcgagaa aggacaaatt ttgtcctttc ttttttgata ttcagagcga taaaaatccg
     8521 ttttttgaag ttttcaaagt tccgaaaacc aaaggcattg cgcttgataa gttt
//
LOCUS       DAVLVF010000079         8318 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVLVF010000079 DAVLVF010000000
VERSION     DAVLVF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.079"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..259
                     /locus_tag="VYN66_001658"
     CDS             <1..259
                     /locus_tag="VYN66_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEV1495649.1"
                     /translation="MNRAISCYREEQSGCLDMVVIQKQNEIEELSTNIEKKIMNYIFE
                     DDGNVSEVIGALDIIHHLDKIAHTTQSIYKWIMYRKYGNIN"
     gene            272..1747
                     /gene="galT"
                     /locus_tag="VYN66_001659"
     CDS             272..1747
                     /gene="galT"
                     /locus_tag="VYN66_001659"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1495650.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGTDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYLYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFRFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1760..2740
                     /gene="galE"
                     /locus_tag="VYN66_001660"
     CDS             1760..2740
                     /gene="galE"
                     /locus_tag="VYN66_001660"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1495651.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKPALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGLPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWN"
     gene            2950..3609
                     /locus_tag="VYN66_001661"
     CDS             2950..3609
                     /locus_tag="VYN66_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV1495652.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3634..4701
                     /locus_tag="VYN66_001662"
     CDS             3634..4701
                     /locus_tag="VYN66_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1495653.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELASKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWGVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            4804..5814
                     /locus_tag="VYN66_001663"
     CDS             4804..5814
                     /locus_tag="VYN66_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495654.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            5826..7517
                     /locus_tag="VYN66_001664"
     CDS             5826..7517
                     /locus_tag="VYN66_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1495655.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7520..8230
                     /locus_tag="VYN66_001665"
     CDS             7520..8230
                     /locus_tag="VYN66_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1495656.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2819 a         1156 c         1639 g         2704 t
ORIGIN      
        1 tatgaatagg gctatatctt gttatagaga ggaacaatct ggatgtttag atatggttgt
       61 tatacaaaaa cagaatgaga tagaagagct tagtacaaat attgaaaaga aaataatgaa
      121 ttacatattt gaagatgatg ggaatgtttc tgaagtaatt ggtgctttag atattattca
      181 tcaccttgat aaaattgctc atactacgca atcaatttat aaatggataa tgtatagaaa
      241 atatgggaac ataaattaga aagaaaaata tatgaaaaca attattgata attttgttga
      301 tagagtaatt gaatacggaa tgtataatga aattgacaaa atatatgtga aaaatagagt
      361 actagcgtta ataggtgagg agggaactga tcgcatttcc gatgaaaatg acctgaaaca
      421 aataaaagat tacttagtgg aaatcgcctt aaaaaacggt aaaattaaag atttaatcga
      481 ggaaaaggaa tgtttaggag cagagttaat gaatttcata gttcctttac ctagtcgatt
      541 aaatgatatt ttttggagtt catatgatat atcacctcaa gaagcagttg aagagtttta
      601 taagttaagt aaagatagtg attatattaa aacttctgct attgctaaga atattgagtt
      661 tcgagcatca actaaatacg gagagctaga gattacgata aatttgtcaa aacccgaaaa
      721 agatccaaaa acaattgctg ctgaaaaatt agtaaaagcc actaattatc ccaaatgttt
      781 actatgtatg gaaaatgaag gatatcaggg gcgaataaat tatccagcac gttcgaatca
      841 tagaattatt cgattgaaac ttggtgacga agtatgggga ttccaatatt ctccctactt
      901 atactttaat gagcatgcga tatttttaaa tagtcaacat gtaccaatgg ctattacctc
      961 taaaacattt gaacaattat tggagattgt tgatattttg ccaggatatt ttgctggttc
     1021 aaattctgat cttcctatct caggaggctc cattcttagt cataaccact accagggagg
     1081 gaaacatatt ttcccaatgg aaaaagcaaa atttgagagt gaatttcgtt ttaaagactt
     1141 tgaggatgtt aatgctggta ttgtaaaatg gccaatgtca gtaattaggt tacaaagtga
     1201 aaataaaaat cgcttgttag atttagctac taaaattcta aataagtgga gagaatattc
     1261 agatttagaa gtagacatca ttgctatgac tgaagatgtc ccacaccata cggtgactcc
     1321 tattgctcgt aaagtggacg ggagatatga attagatatt gttctaagag ataatcatac
     1381 tactgaacag tacccagatg gtgttttcca tcctcatcaa gatgtacaac atattaaaaa
     1441 ggaaaatatc gggttaatcg aagtgatggg acttgcgata ttaccacctc gtttgaaacc
     1501 agagttagaa gaagttggaa aatatctttt aggagaggat aacgcaattg cggattatca
     1561 tttagaatgg gccgaccaat tgaaagaaaa atatcctcgc attaataaag aagaggttaa
     1621 tagcgtagtt caacacgaag caggacaagt gtttgcgaga gttctggaag atgcaggagt
     1681 ttataagaat acaccgtctg ggcatgaagc gtttatgaga tttgttaagt cggttggaat
     1741 taattaggag gaaacaaaaa tggcaatatt ggtaacaggc ggagctggtt atattggtag
     1801 ccataccgta gtagaattac taaatttagg aaaggaagtc atcattgtcg acaacctttc
     1861 gaactctagc atcttggtgc tagaccgtat tgaagcaatt acaggaatac gtcccgtgtt
     1921 ttacgaatta gatgtttgtg ataaaccagc attgagaaag gtatttgaac aagaatcgat
     1981 tgatgctgca attcattttg caggttataa agctgtcgga gaatccgtgc aaaagcctgt
     2041 gatgtactac aaaaataata ttatgagtac attggcactt gttgaagtga tgtcagaatt
     2101 taatgttaaa aagattgtct tttcttcaag tgcgactgta tatggaatta acaatcagtc
     2161 acctctaatt gagacgatgc aaacaagtgc gacaaatcct tatgggtata cgaaagtgat
     2221 gcttgagcaa attttaaaag atgttcatgt ggcagattca gaatggagta ttgcgttgct
     2281 tcgttatttc aatccaattg gtgctcatga gtctggtttg attggggaag atccctcagg
     2341 acttcctaac aacttgatgc cttatattgc acaagtagcg gtaggtaagc tatcagagct
     2401 aagtgtgttc ggaaatgatt atgatacgct tgatggtact ggtgtgcgtg attatatcca
     2461 tgtagtagat ttagcaatag gacatataaa agctttagaa aaagtatctg aaaaaacaga
     2521 tgtttatatc tataacctag gctcgggaga aggcactagt gtattacaac ttgtaaatac
     2581 atttgaaagt gttaataaga tccctattcc ttataaaata gttccaagac gttcaggaga
     2641 cgttgcaact tgttatgcaa atgcagacaa agcatataag gaattaaatt ggaggacaac
     2701 aaaatcgatt gaagacatgt gtagagatac atggaattag caatcaaaaa atcccaatgg
     2761 ctataattga tgatactgtc tatatgagtg ctgtattttg gaaaaattac ttaacctttt
     2821 aatgaaatgc atatttaatg aaagataaat taaaaaaaat gtttgacagc gcttgacaaa
     2881 ggaaacggtt tcattgtata atgtaaacat agtgacaccg atgtcaaata taaaaaagaa
     2941 ggtctttata tgaaaaactt aacaaaaatt aagttcaaag aaaatgggga atttaatcat
     3001 ttccctggga atacagttgt agcaaatctt tatactaaac aagatttgat ggaagttgtt
     3061 gatattattc aatcacgtta tagagaatta ccatttatcg ataagtttac tttaactcca
     3121 aggaattcta ttcacatgac tgtaattgaa ttgttgtgcc atgaaaatcg tgaaacggag
     3181 ttttggagca gtaatcttcc tctagataca cctttacagg aaatacatga ttactttgct
     3241 aaacaacttg aaatttttcc attgttggat gaagaaattc atatgcgtat aactgaaatg
     3301 ggaaaacaaa acatactagt tgaacctgca gatgaagctt ctgcaaagag attagaagaa
     3361 attcgtactt atgtctcaga aaaagcaggt gtttgtttcc ctaatcatga tagatatcaa
     3421 ttccatattt caattgggta tcttcggatt cctctaaccg aagaggagga agaagagttt
     3481 actaaagtca gagcagaatt aactgaaatt ttattagaga agattccaac tattactgta
     3541 aaccgtattg actatactgt atttgaagat atgagacaat ttgttccata tcacgaaaaa
     3601 tttaaataaa ataataaaaa aggagaactt atcatgaaaa aaatgaaagt ttggtctact
     3661 gtacttgcaa cgggagttgc tcttactaca cttgctgctt gctctggagg ttcaaattct
     3721 acgactgctt cttcatctga agaaaaagct gataaaagtc aagaattagt tatctattcg
     3781 aactcagtct caaatggtcg tggtgattgg ttaactgcta aagcaaaaga agctggtttt
     3841 aatataaaaa tggttgatat cgctggcgct caattagcag accgtgttat tgctgagaag
     3901 aataatgcag ttgcagatat ggtatttgga attggtgctg ttgactcaaa taaaattaga
     3961 gatcaaaaat tactagtaca gtacaagcct aaatggttag ataaaattga tcaatcttta
     4021 tcagataaag ataattatta taatcctgtg attgttcaac cattagtttt aattggggcg
     4081 cctgatgtaa aagaaatgcc taaagactgg acagagttag ctagtaagta taaaggtaaa
     4141 tattcaattt atggtcttca aggtggtaca ggacgggcaa ttctagcaag tatcttagtt
     4201 cgttaccttg atgaaaaagg agatttaggc gtatcagaaa aaggatgggg agttgcaaaa
     4261 gaatactttg caaatgccta tactcttcaa aaaggtgaaa gctcagttgt taaagcgtta
     4321 gataaagaaa gcccaattca atacggtatg atctggggtt ctggagcgtt aatcggacaa
     4381 aaagaacaaa atgttgaatt caaagttatg actcctgaga ttggtgtacc atttgtaact
     4441 gaacaaacta tggttttaag cactagtaaa aaacaagcgt tagctaaaga atttattgat
     4501 tggtttggtc aatcagaaat tcaagtagaa tatagtaaaa aatttggatc tattcctgca
     4561 aataaagatg ccctaaaaga gttacctgaa gatacgaaaa aatttgttga tcaagtgaaa
     4621 ccacaaaata ttgactggga agctgttgga aagcatttgg atgaatgggt agaaaaagct
     4681 gaattagaat acgtaaaata agaaataaga gttatttttt atgtccaagt ctctctatta
     4741 tttctaaatt taaggaagaa ataaagagag gcttttggtt tagtaataga gggagttagt
     4801 gagatgatta aatttgataa tattcaaatt aaatatggtg attttgttgc aattgataat
     4861 ctgaatttag atatacatga aggggaattc tttacatttc ttgggccttc aggatgtggt
     4921 aaatcaacta ctttgagagc attggtaggt tttttagatc catcatcagg aagtattgaa
     4981 gttaatggaa cagatgtcac tcatttggaa cctgaaaagc gtggaattgg tattgtattt
     5041 caatcttatg cgctatttcc aactatgact gtttttgata atattgcatt tggtttaaaa
     5101 gttaagaagg tagctccaga tgttattaaa gctaaagtat cagcagtggc agcaaaaatt
     5161 aagatctctg atcaacagtt acagcgtaat gtatcagaat tatctggggg tcaacaacaa
     5221 agggtagcat tggctcgtgc tctggttctt gaacctaaaa ttctttgtct agatgaacca
     5281 ttgtcaaacc ttgacgcaaa attacgtgta gatttgagaa aagagttgaa aagacttcaa
     5341 aaagagttag gtattactac tttatatgtt actcatgatc aagaggaagc cttgacttta
     5401 tctgatagaa ttgcagtctt taacaatgga tacatcgaac aggtcggtac accagtagag
     5461 atttatcata attctcaaac tgaatttgta tgtgatttta ttggagatat taatgttttg
     5521 accgatgaaa cagtccacga agtattactg aaaaatacaa gcgttttctt agaggataaa
     5581 aaaggttaca ttcgattaga gaaagttcga ttcaatcgtg aaactgaaca agattttatt
     5641 ctaaaaggga caattattga tgttgagttt tctggagtta caattcacta tacaataaaa
     5701 gtttctgaaa gtcagattct taatgtaaca agtattgata gtcaggctgc tattagatct
     5761 gtcggagaaa gtgtggaatt atttatcaca ccatcagacg ttctgcaatt ttaaggaggt
     5821 gcagtatgcg tcataaatta aatttaaaag attggcttat tcgtttaggg ttaatctggt
     5881 tcttagtaac atttattatt tatccaaact ttgatctagt agtgaatgta tttgtaaaag
     5941 gaggagaatt ttcccttgat gctgtacatc gtgttctaaa atctcagagg gcacttcaga
     6001 gtattatgaa cagttttaag ttagcatttt cactcattat tacagttaat gtcgtaggta
     6061 ttctttgtgt tctatttaca gagtactttg atattaaagg tgctaaaatt ttaaaattag
     6121 gttatatgac ctctttaatt tatggaggag tggttttagc gactggatat aaatttgtct
     6181 atggtcctta tggattgatt acaaaatttt tacaaaatgt tatcccttct ttagactcta
     6241 actggtttat tgggtatggt gcagtcttat tcattatgac attttcagga actgctaatc
     6301 atacattgtt tttaacaaat acaattcgaa gcgttgacta tcacactatt gaggctgctc
     6361 gaaatatggg agcaaaacca tttactgttt tccgaaaagt agtgttacca accttaattc
     6421 caactctatt tgcacttact attatggttt ttcttagtgg tttatctgca gtagcagcac
     6481 ccatgattgt tggtggtaaa gaatttcaaa ctataaatcc aatgattatt acatttgcag
     6541 ggatggggaa ttctcgtgat ttagctgcct tacttgcaat tattttaggt attgcaacta
     6601 caattttgct tactatcatg aataagatag aaaaaggtgg aaattatatt tctatctcta
     6661 agactaaagc gcctcttaaa aaacaaaaaa ttgcgtctaa gccttggaat atcattgctc
     6721 acattgtagc atatggattg ttcacagttt tcatgcttcc actaattttt atagtattat
     6781 actcatttac agatccagtt gcaattcaaa caggtaactt aacattatca aactttactt
     6841 tagaaaatta tcgcttattc tttagtaata gtgcggcatt ctctccattc ttggtcagct
     6901 ttatttattc tattattgct gcgacaacag caacaattct cgcagttgta tttgctcgtg
     6961 ttgtcagaaa acataaatct cgttttgatt tcttatttga atatggtgct ctacttcctt
     7021 ggttactacc aagtacactt ttagcagtaa gtttattatt tacttttaat cagccacaat
     7081 ttcttgtctt gaatcagatt ttggtaggta gtttggtaat tctacttatt gcatatatag
     7141 ttgtaaaaat cccattttct tatagaatgg tacgtgctat tttatttagt gttgatgatg
     7201 agatggaaga tgcagcaaga agtatgggtg cttcaccttt ttatactatg atgaaggtta
     7261 tcattccatt tattttaccg gttgttctct ctgttattgc tttaaacttt aactctttat
     7321 taactgactt cgacttatct gtattccttt accatcccct agctcaacca ttaggtatta
     7381 cgattcgatc tgcaggtgat gaaacagcaa catctaatgc acaagctctg gtatttgttt
     7441 atacaattgt tctgatgatc atttctggaa cggtattata cttcacacaa agaccggggc
     7501 gtaaagtaag gaaataatca tgacagccac tagtcttggg ttatcaaata ttgaaatagt
     7561 tgtcaggatt gttttatcag tagtcattgg tagtataatt ggtttagaga gagggagcaa
     7621 atcccagcct gcaggcatcc gaacttatag tattgtttgt ctagctgcat gtttgattat
     7681 gatgacgaat gaatacgtat cttataaatt tgggacagga gatcctacac gattaggagc
     7741 tcaagttata tcaggtgtgg gttttctagg cgctggaacg attcttatta cagataaaaa
     7801 gaaaattaca ggtctgacaa ctgcagcagg catttgggct tcggcaggaa ttggattagc
     7861 tattggagta ggtttttatg agggagctct tttagtagcc atttctgttt ggggtgtgat
     7921 atccatgttc caaccactaa aaaaatatct gcaaaatcgt tctaaaatga ttgaattgta
     7981 tatagtggtt aaatctacag aagcctataa tcgtgtattg gtttattgtg cagaaaatgg
     8041 tatcagaatg accgattcaa gaacagcgtt tggagatgtt aattctgaca gaattgaata
     8101 ctttgatgtt ccggacaaaa aaatagcgtc atttattact ctggaattat caggtagatt
     8161 tgaacatctt cgactgatgg aagaaattgc taatattgta ggtgttattt atgtcgaaga
     8221 aattagctga aatttagtaa acaaagggac agatgttttt gtccttttgt tttttagatg
     8281 ttttaatcat ctaatttttg agtagatttt tgctcaga
//
LOCUS       DAVLVF010000080         8245 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVLVF010000080 DAVLVF010000000
VERSION     DAVLVF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.080"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..431
                     /locus_tag="VYN66_001666"
     CDS             <1..431
                     /locus_tag="VYN66_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1495657.1"
                     /translation="RYASVNKHFSKVCDFLENTNLTDLVDGKIDIDGENVFANCMTYL
                     ADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDIAFYDSKDYQI
                     VELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            433..2655
                     /locus_tag="VYN66_001667"
     CDS             433..2655
                     /locus_tag="VYN66_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEV1495658.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPELVQPV
                     APTTSISEVQHKSGNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     NELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNHLVTRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPAPLEWPRDVGGRDLQISGGATYIDSVIVEKNNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLKKQGDTDYNYTIREHGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTQVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQISIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            2695..3804
                     /locus_tag="VYN66_001668"
     CDS             2695..3804
                     /locus_tag="VYN66_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1495659.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEYEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            3961..4851
                     /locus_tag="VYN66_001669"
     CDS             3961..4851
                     /locus_tag="VYN66_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1495660.1"
                     /translation="MKIIGIDIGGTTIKADLYDDFGTSLNQFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYVLNYSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTSEIEKRF
                     GLSTFVENDVNCAALGELWEGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLSSGDKVAKETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILAKKDILLPEIQSSLAKNAMDNRFLPKNVVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            4876..5199
                     /locus_tag="VYN66_001670"
     CDS             4876..5199
                     /locus_tag="VYN66_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495661.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELISIESNLEMKQQNLIDEVNKTIKENDAKIQYISSSRRGEFVEKIIGRVV
                     EKYGY"
     gene            5189..7180
                     /locus_tag="VYN66_001671"
     CDS             5189..7180
                     /locus_tag="VYN66_001671"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEV1495662.1"
                     /translation="MAISQMKRISLLFTKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFSTIQISHTSNSHHGVIEGNDALNYLMNKQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERNIQSNNEEIERLIKW
                     EKLEIIPAHLEQFSFCRGKVGTIPRTVDNRLYNNFLENNIEVQEIFSNDREYGVVVFY
                     QSSCSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKNLID
                     LQWQIDYILSIYARQISKNNLLSTPHLVALEGWIEETRILYFIKVMDERFGHSIYIYE
                     SETLSDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGIMLALKIFHLPSVTKKFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ITTTSDVMTILVVSVVFGFITVFVGLLASGLQKVRMKKYAEAYNSGFAWCVILLGLLF
                     IAVGMLMPDIRPLFVLGKWVSIFNAVGILIASIIQAKSLSGIGSGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGTLAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            7196..7672
                     /locus_tag="VYN66_001672"
     CDS             7196..7672
                     /locus_tag="VYN66_001672"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEV1495663.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            7706..>8245
                     /locus_tag="VYN66_001673"
     CDS             7706..>8245
                     /locus_tag="VYN66_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495664.1"
                     /translation="MSNLENLRESVIEQAHEKGSMKLLDSKKKIDDEFEMQKSVIVKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALIEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNHLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISK"
BASE COUNT         2842 a         1145 c         1655 g         2603 t
ORIGIN      
        1 gacgatatgc ttctgtcaat aaacattttt caaaagtctg tgatttttta gaaaatacaa
       61 atttgacaga tttagttgat ggaaaaattg atattgacgg ggaaaatgtt tttgcaaatt
      121 gtatgactta tctagctgat ggagttccag gagatatttt tgaaactcat aagaaatatt
      181 tagatattca tattgttgtt gaaaatacag aaaaaatggc agtaacttcc ccagttcgtg
      241 ctcagtcacg tgtacctttt agcgaagaga aggatattgc tttttacgat agcaaagact
      301 atcaaattgt tgaattgctt ccagggaata tgttggtgac ttttgaagaa gatcttcacc
      361 aaccaaagat tcattgcaat gatgaaactg ttaaaaaact tgttatcaaa gttttaaacg
      421 aggaaaaata aaatgaaaaa aaatattaaa caatatgtaa ccttaggtac tgtagtggta
      481 ttatcagcat ttgttgctaa ctcagttgca gctcaggaga ctgaaacttc tgaagtatca
      541 acaccagagt tggtgcaacc tgttgcacca acgacttcga tttcggaagt acaacataaa
      601 tcgggtaact cttcggaagt tactgtacaa cctcgaacag ttgaaactac tgttaaggat
      661 ccatcttcta cagcggaaga aactcctgtc ttagaaaaaa ataatgttac tttaacaggg
      721 ggcggagaaa atgttactaa tgagttaaag gataaattta ctagcggtga ctttactgta
      781 gtgattaagt acaatcagtc tagtgagaaa ggtttacaag ctctgtttgg aatatctaat
      841 tctaaacccg gtcaacaaaa tagttatgta gatgtgttcc ttagagacaa tggtgaattg
      901 ggaatggaag cgcgtgatac ttcttccaat aaaaatcacc tagtaaccag acctgcttca
      961 gtttggggta agtacaaaca agaggccgtg acgaacactg ttgcagtagt agcagattca
     1021 gtcaaaaaaa cgtattcttt atatgcaaat ggtacaaaag tagtagaaaa gaaagtggat
     1081 aacttcctaa acatcaagga tattaaaggt attgattact atatgcttgg gggagtgaaa
     1141 cgtgcaggaa aaacggcgtt tggttttaac ggaacactag aaaatatcaa attctttaat
     1201 agtgcattgg atgaagaaac tgttaaaaag atgacaacaa acgctgttac tggacattta
     1261 atttatacgg ctaatgatac aacaggttct aactatttcc gtattccagt tctgtatact
     1321 tttagcaatg gtcgggtttt ttcaagcatt gacgctcgtt acggtggaac tcatgatttc
     1381 ttgaataaaa ttaatattgc tacaagttat agtgatgata atggtaagac atggactaaa
     1441 ccaaaattaa cattagcatt cgatgatttt gcgccagcac cattagaatg gcctcgtgat
     1501 gttggtggac gtgacttaca aatcagcggt ggtgcaacct atattgactc tgttattgtt
     1561 gaaaaaaata acaaacaagt actcatgttt gctgatgtta tgcctgctgg agtaagtttt
     1621 agagaagcta ctagaaaaga ttcaggttat aaacaaattg atggtaatta ttaccttaaa
     1681 ttaaagaaac aaggtgatac tgattataat tatactattc gtgagcatgg tactgtatac
     1741 gacgatcgta ccaacagacc aactgaattt tcagtagata aaaatttcgg tattaaacaa
     1801 aatggtaatt atttgacggt agagcaatat tcggtttcat ttgaaaataa taaaaagact
     1861 gaatatcgta atgggactca agtccatatg aatatcttct acaaagatgc attattcaaa
     1921 gtagtgccaa cgaactatat tgcatatatt tctagcaatg atcatggcga atcttggtct
     1981 gcaccaactt tattacctcc tataatggga cttaatcgga atgcgcctta tttgggtcct
     2041 ggacgtggaa tcattgaaag ctcaactgga cgtattctta ttccgtctta cactggtaaa
     2101 gagtctgcat tcatttatag tgacgataat ggagcgtctt ggaaagttaa agttgtgcca
     2161 cttccttcta gttggtcagc agaagcacaa tttgtagaat tgagtccagg agtaattcaa
     2221 gcatacatgc gtacaaataa tggtaaaatt gcatatttaa caagtaaaga tgcaggtact
     2281 acttggagtg cgccggaata tttgaaattt gtttcaaatc caagttatgg aacacaatta
     2341 tcaatcatca attatagtca attgattgat ggtaaaaagg ctgtcatttt aagtactcca
     2401 aactccacaa atggtcgtaa acacggacaa atttcgattg gtctaattaa tgatgataat
     2461 acaattgatt ggcgttatca tcacgatgtt gattatagta actatggata ctcatattca
     2521 acattgacag agttaccaaa tcatgaaatt ggattgatgt ttgaaaaatt tgattcatgg
     2581 tctcgtaatg aacttcatat gaaaaatgtt gtaccatata taacatttaa gattgaagat
     2641 ttgaaaaaga attaaagctg aaatttaaaa atatatagaa agaggataaa aattatggta
     2701 aattacggta ttgttggagc tggatatttt ggagctgatt tggctcgctc aatgaacaaa
     2761 attgaagatg caaaagtggt tgcggtattt gacccaaatc atggagaaga agttgctcaa
     2821 gagttgggat cagatgtttg tgcaagttta gatgaacttg tagcacgtga agatattgat
     2881 tgtgtgatcg tagcttcacc tagctacctt caccgtgaac ctgttgtgaa agctgctcaa
     2941 catggcaaac acgtattttg tgaaaagcca attgcattgt cttatgaaga ttgtaaagcc
     3001 atggttgacg catgtaaaga aaataatgtt atctttatgg ctggtcacat catgaacttc
     3061 tttaatggtg tacaccatgc taaagaattg attactcaag gtaaaatcgg taaagttctt
     3121 tattgccatg ccgctcgtac aggttgggaa gaacaacaac caactgtatc atggaagaaa
     3181 cttcgttctc aatctggagg acatttgtac caccatattc atgaattaga ttgcattcag
     3241 ttcatcatgg gaggacttcc tgaaaaagcg acaatggtag gaggcaatgt atatcataaa
     3301 ggtgaaaact ttggtgatga agatgatatg ctcattgtaa acctagaata ctctgatgat
     3361 cgttatgctg ttttggaata tggtaatgct ttccgttggg gtgaacacta cgtcttgatt
     3421 caaggaactg aaggagctat caaacttgac ttgttcaata ctggaggtac tcttcgtgtt
     3481 aaaggtgaag gagaatcaca cttcttagta catgaaactc aagaggaaga tgatgaccgt
     3541 acagctatct ataccggtcg tggtatggat ggagcaattg cgtacggtaa accaggagta
     3601 cgttgcccat tatggttaca aacatgtatt gataaagaaa tggaatatct acatgacatc
     3661 attaaaggtg gagaaattac agaagaatat gaaaaacttc tcaatggtgt agctgcttta
     3721 gaatcaatcg ctaccgctga tgcatgtact ttatcagtta aagaagatcg aaaagtaagt
     3781 ctttcagaaa tcacaaatgc ttaactattg taaaacagaa tagtaaattc ttgtcattat
     3841 ataatttcta aagttctgtg atacaactca ttgaataaag aaatagagat gggactggga
     3901 taatgcccag tcccattttt tatcaaaaag taatgagatc aaaaatgtgg gagtgatgaa
     3961 atgaagatta tagggatcga tattggcgga acaacaatta aggcagattt atacgatgat
     4021 tttggaacga gtttaaatca gttcaaagag atagaaacaa ttattgacta tgatttggga
     4081 acgaaccaga tattaaatca ggtctgtgat ttaattggtg agtatgtttt aaattattca
     4141 attgatggtg ttgggatttc cactgctgga gttgttaatg ctaatacagg agaaatcatc
     4201 tatgcaggct atacaatacc agggtatatc ggagtaaact ttacttccga aatagaaaaa
     4261 cgttttgggt tgtctacttt tgttgaaaat gatgttaatt gtgctgcatt aggtgaattg
     4321 tgggagggac aagccaaaga taagaaaaat gtagtaatgg ttactattgg aacaggtata
     4381 ggaggcagta ttattgtcaa tggacaaatt gttaacggat ttaactatac tgctggtgaa
     4441 gtaggctata ttcctgtagg taattcggat tggcaaagca aagcctcaac aaccgcattg
     4501 attcatttat atcaaaagaa gagcttgaaa actaatcaaa ctggacgtac tttctttact
     4561 gatttaagtt ctggagataa agtcgctaaa gaaacttttg aaatttttgt agaaaatcta
     4621 acaaaaggtt tattaactat ttcttatcta cttaatccag aaattctcat attaggaggt
     4681 gggattctgg ctaaaaagga tattttgtta cctgaaattc aaagttcttt agctaaaaat
     4741 gcaatggata ataggttttt gcctaaaaat gttgtggcag ctacattagg aaatgaagct
     4801 ggtcgtatag gagctgtaaa aaatttctta gatagaattt ctaataaata gtgtgaaaga
     4861 taaggaggtg tcacaatgac taactctgta ttttcgacaa tgcaagatat tgagaatgtt
     4921 gcaaccgata ttataaaatc atatgataat gagatttata cgtataaagc tgtctcccaa
     4981 gaagaattgg aaaaactaga aaaaagttat gatgaaaaaa gtcacgaaga attaatttca
     5041 atagaaagca atttagaaat gaaacaacag aacctcattg atgaggttaa taaaacaatc
     5101 aaggaaaatg atgcaaagat tcagtatatt tcatcaagta ggagaggaga atttgtagaa
     5161 aaaattattg gtagggtggt agaaaaatat ggctattagt cagatgaaaa gaatttctct
     5221 actatttact aagagtagtc ttgatgatgt tttaaaaact attcaagaac tagagtcagt
     5281 gcagttccgt gatttaaagg ttcaggataa ctggtcagaa gcgctagaaa aagatgaggt
     5341 tgtattttca actattcaaa tttctcacac ttctaattcc catcatgggg ttattgaagg
     5401 aaatgatgcc ttgaattatt tgatgaataa acaacaacat ttggaagcaa ctgtagagaa
     5461 attacaagaa tacctaccga aagaaaacac gtttaagtta ttacagcaac ctccgataac
     5521 aacctcttat gaagaattag aaaaatttgg taaagctaat gttgctgagg gtgttcttaa
     5581 aaaagtgaat catcaaataa acagagttca tgaattagaa agaaacattc aaagtaataa
     5641 tgaggaaata gagagattaa taaagtggga aaaattagaa attattcctg cgcatttaga
     5701 acaattttct ttctgtagag gaaaagtcgg aacaattcca aggactgtag ataatcgctt
     5761 atacaataat tttttagaaa acaatattga agttcaagaa atattttcta atgatagaga
     5821 gtacggtgtt gttgttttct atcagtccag ttgctctatt gattttgatg aatacttatt
     5881 tgaaccattt gattattcta gaaaggaatt accgaagcag cgagtagtag atttggatca
     5941 agaaaacatg cagttaataa ctgaaaaaga gaatattatc gcatcgttgc aagattcaaa
     6001 gaaaaatttg atagatttac aatggcaaat tgactatatt ttatctatct atgctcgtca
     6061 aatctctaag aataaccttt tgagcactcc gcatctagtt gcattagaag gatggataga
     6121 agaaactcgt attttatatt ttataaaagt tatggatgag cgttttggac attctattta
     6181 tatttatgaa tcggaaacat tgtcggataa tcaagatgaa atacctatca aattaacgaa
     6241 tcactcttta attgaaccat ttgaattatt gacagaaatg tatgctctgc ccaaatatta
     6301 tgagaaagat cctacacctg tattagcacc attttacttt acattttttg gaatgatggt
     6361 tgctgattta ggctatggtt tactattgtt tttaggaata atgttagcat taaaaatttt
     6421 tcatctacct tcagtaacta agaaattttt aaaattcttt aatatattag gggtagccgt
     6481 tgcaatttgg ggtggaatct atggctcatt ttttggatat gagttgccat ttcatctgat
     6541 aactacaacc tcggatgtca tgactatatt agtagtgtca gttgtgtttg gatttattac
     6601 agtatttgta ggtttgttag cttcaggact acaaaaagta agaatgaaga aatatgcaga
     6661 agcatataat tcaggatttg cgtggtgtgt tattctgctt ggcttgttat ttattgctgt
     6721 cggaatgttg atgcctgata ttagaccgtt atttgtatta gggaaatggg tatctatttt
     6781 taatgctgtg gggattttga ttgcttctat tattcaagcc aaaagcttgt caggtattgg
     6841 ctcaggattg tttaatctat ataacatttc atcttatata ggtgatttag ttagtttcac
     6901 tcgattgatg gcattaggat tatctggagc aagtatagca tcagctttca atttaattgt
     6961 tggtttgttt ccggggacat tggctaaact gacaattgga ttagtattat tcattctttt
     7021 acatgcgatc aatatttttc tatcgttact atcaggatat gttcatggag cgcgtctgat
     7081 atttgttgaa ttttttggta agttttatga gggtggagga aaaccatttc aacctttgaa
     7141 ggcttctgag aaatatatta aggttattac aaagaattaa tggaggatat atataatgga
     7201 acatttagca acttattttt caacctatgg aggagctttc ttcgctgcat tgggaattgt
     7261 attggcggtt ggattaagcg gtatggggtc tgcttatgga gttggtaagg ctgggcaatc
     7321 tgccgcagct ttactgaaag aacagccaga aaaatttgcc tcagctttga tattgcaact
     7381 attgcccgga acacaaggat tatatggttt tgttattgga attttgattt ggttgcaatt
     7441 aactccagaa cttcctttgg aaaaaggcgt tgcttatttc tttgtagctc ttccaattgc
     7501 tattgtagga tacttttcgg ctaagcatca aggaaatgta gcagtagcgg gaatgcaaat
     7561 cttggctaaa agaccaaaag aattcatgaa gggagcaatt ttggctgcca tggtagaaac
     7621 ctatgcaatt cttgcctttg tcgtatcatt cattttgacc cttcgtgtat aagaaattaa
     7681 tttgctattc aaaggaggtg tctaaatgag caatttagaa aacttacgag aatctgttat
     7741 tgaacaagct catgaaaaag ggagtatgaa attattggat tccaaaaaga agattgatga
     7801 tgaatttgaa atgcaaaagt cggtcattgt aaagaaaaaa gaagctgaac atgaacgaaa
     7861 gttaaaagaa ttacaacaga aatatcaaat aatttttcaa caattaaaaa ataaggaacg
     7921 ccaatcaacg ttagtatcaa aacagaaaat attaaaagaa ctttttcaat ctgctttaat
     7981 agaaatggaa tcttggagtg cagataaaga aatggagttc atctatcgaa ttctggaacg
     8041 atattcacaa caagaggtca tagtaacttt tggggagcgg actttagcta aattcaattt
     8101 ggaacaatta gagaaattga aattctcttt tccaaatcat ttatttagtg aacagcctat
     8161 ctcaaatgaa tcaggcttac ttatttcaat aggtaaaatt gatgataact atttgtataa
     8221 aacgttaatt ggatcgattt ctaag
//
LOCUS       DAVLVF010000081         8039 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVLVF010000081 DAVLVF010000000
VERSION     DAVLVF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.081"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            58..1539
                     /locus_tag="VYN66_001674"
     CDS             58..1539
                     /locus_tag="VYN66_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1495665.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1901..2902
                     /locus_tag="VYN66_001675"
     CDS             1901..2902
                     /locus_tag="VYN66_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1495666.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     KVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3053..3970
                     /locus_tag="VYN66_001676"
     CDS             3053..3970
                     /locus_tag="VYN66_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1495667.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAVRLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            3981..4871
                     /locus_tag="VYN66_001677"
     CDS             3981..4871
                     /locus_tag="VYN66_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1495668.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            4900..6516
                     /locus_tag="VYN66_001678"
     CDS             4900..6516
                     /locus_tag="VYN66_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1495669.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVENLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6526..7845
                     /locus_tag="VYN66_001679"
     CDS             6526..7845
                     /locus_tag="VYN66_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1495670.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKIEQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKNNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
BASE COUNT         2700 a         1263 c         1530 g         2546 t
ORIGIN      
        1 ttgtggtata attgcaagag gtttaatccg ataatttata aagagagaaa gatattcatg
       61 agagatttat tatctaaaaa aagtcatagg caattagaat tattagaatt attatttgaa
      121 cataaacgtt ggtttcatcg ttctgaacta gcagagttat taaattgtac agaacgtgca
      181 gtcaaagatg atctatccca tgttaaatct gcttttcctg acttgatttt tcattcttct
      241 actaatggta tacgcattat taataccgat gatagtgata ttgaaatggt ttaccatcat
      301 ttctttaaac attcaactca tttttcgatt ttagaattca tcttctttaa tgaaggttgt
      361 caagctgaga gtatttgtaa agaattttat atcagttcat cttcgctcta tcgtattatt
      421 agccaaatca ataaagtgat taaaaggcaa tttcaatttg aagtcagtct gacccctgtt
      481 caaatcattg gaaatgagag agacattcgt tacttttttg cacaatattt ttcagaaaaa
      541 tattatttcc tagaatggcc atttgaaaat ttttcatcag agccactatc tcaattgtta
      601 gaattggttt ataaggaaac aagctttcca atgaatttgt caacgcatag aatgctaaag
      661 ttgctcctag ttacgaacct atatagaata aagtttggtc attttatgga agtagataaa
      721 gattctttta acgaccaaag tttggatttt ttaatgcagg cagaaggaat agaaggtgtt
      781 gctcagagtt ttgaatcaga atacaatatc tctttagatg aagaagttgt ttgccaatta
      841 tttgtgtctt actttcaaaa aatgtttttc atagatgaaa gtctctttat gaaatgcgta
      901 aaaaaggata gttatgttga aaaatcttac catctattga gtgattttat tgatcagatt
      961 tcagtcaagt atcagattga aatggagaat aaggataatc tgatttggca tctgcataat
     1021 accgcacatc tgtatcgtca agagttgttt actgagttta ttttatttga tcaaaaaggg
     1081 aatacaatca ggaactttca aaatattttt ccaaaatttg tttcagatat aaaaaaagag
     1141 ctttctcact atttagagac tcttgaggtt tgttctagtt caatgatggt aaatcatcta
     1201 tcttatacct tcatcactca taccaaacat ttggtgatta atttgctaca aaatcagcct
     1261 aaactaaagg ttttagttat gagtaatttt gatcagtatc atgcaaaatt cgttgcagag
     1321 acactttctt attactgtag caataatttt gaactggaag tttggactga attagaatta
     1381 tcaaaggaat ctttagaaga ttcaccttat gatatcatta tttctaattt tattattcct
     1441 cctattgaaa ataagagact catctattca aataatataa acacggtctc actcatatac
     1501 ttgttaaatg ccatgatgtt tattcgatta gatgagtaac caaaaataac aaaaaaacgc
     1561 tatttcgcgt ttgaactgta cctcaaaagt tagacagaaa aaatctaatt tttggggtgt
     1621 ttttgttaag aaaagacctt caatcattta agcaaaatgt acatctagat ttatagccct
     1681 ctaaaactct tgcttttttt gtagaagata cgaaaaaagc cataaaacca aggcttttcc
     1741 tgttttatgt agatgtttac acatgaaaat tgaagtattt tcaaaagcta attggagtga
     1801 ttttattcat gatgggataa atgatctggc caaaaatgat tgagaaatag ttgcttaaaa
     1861 agcatttgca tgatactata tattgaaagg aggtgataat atggtagaac aaagaaaatc
     1921 aattaccatg aaagatgttg ctttagaagc aggagttagt gttggaactg tttcacgtgt
     1981 aattaataaa gaaaaaggca ttaaagaagt aactttgaaa aaagtggaac aagcgattaa
     2041 aactttgaat tacattccag attactatgc tagaggaatg aaaaaaaatc gaacagaaac
     2101 gattgcaatc attgtaccaa gtatctggca tcccttcttt tcagaatttg ctatgcatgt
     2161 ggaaaatgaa gtctataaga gaaataacaa attactctta tgttctataa atggtacaaa
     2221 tagagagcaa gactatctgg agatgttgcg tcataataaa gttgatggag tggttgccat
     2281 tacctatagt ccaattgaac attacttgac gtcaggaatt ccctttgtta gtattgaccg
     2341 cacatactca gatattgcca ttccttgtgt ttcatcagat aatgatgcag gtggaagaga
     2401 agcagctaaa caattaatta gtaaaggatg tcagcattta gcctttgtgg gcggacataa
     2461 tacaaccatt aatgaaacca aacgtcgtag aatttcattt gaaaagtatg tccaagagca
     2521 taaaatacct cttagtattt ttgatttgga tgagaaagtt actgactatt atggaaagct
     2581 agagagtttc ttggaagaaa attcttctat agatggaatt tttacaatca acgattttac
     2641 agctttggat gtgattgaaa tactagaaaa aaatggcaaa cgtattcctc atgatgtcca
     2701 aattattgga tacgatggaa ttaaaatggc tggagataga gattatctac tttcaactat
     2761 tgaacaacca ttggaagaaa tggcaaaaga agcggttcgt attttgtttg atataattga
     2821 tggaaagact gttaatttgc agacaatcct accagtaaaa tttgttgaag gaaaaacaac
     2881 aaaaaatgaa aataagtctt gacagaaagc gctatcaatg atagaatgaa ttcagataaa
     2941 aagatttatt tttaaaacaa aaatgaaacg tttcaaaaaa agaaataaaa agacagcgcc
     3001 aagcgctatc ttttatagaa aaaatgaaac gtttcaaaaa aaggaggttg ctatgaatag
     3061 caaagcgaag caagtttctc tttgggaaag aatcaagaaa caaaaactct tgttattgat
     3121 gactgtcccc ggtttagttt taacctttat ctttaaatac atccctatgt atggggtttt
     3181 aatcgcattt aaagattaca atcctttaaa aggaatttta gggagtgatt ggattggttt
     3241 ttctgagttt acaaaattca tatcctctcc caactttggt atcttgttag ccaacacatt
     3301 aaaattaagt atctatggtt tattgcttgg ctttttacca ccaatcattc ttgcgattat
     3361 gctcaatcaa ctcttgagtg aaaaagtcaa aaaacgaatt cagctcattt tatacgcacc
     3421 aaactttatc tcagtcgttg ttattgtcgg tatgattttc ctcttctttt cagtgggagg
     3481 accaatcaac aattttcttt ctatgtttgg aatgaaggct gacttcttga caaatccaga
     3541 cttctttaga cccttataca tctttagtgg tatctggcaa ggaatgggct gggcttcaac
     3601 gctctacacg gcaacattgg taaatgtaga tccagcctta gtagaagcag ttcgactgga
     3661 tggagccaat atcttccaac gaatctggca cattgatatt ccagctctta agcctattat
     3721 ggttatccaa tttgttttag ctgcaggtgg aattatgaat gtcggatatg aaaaagcatt
     3781 cttgatgcag acatcgttaa atttgccaac ttctgaaatt atctcgacat atgtctataa
     3841 agttggtctt gtatcaggag actattctta ctcaacagcg gttggtttgt ttaatgcagt
     3901 gattaacgta gtattgcttg ttgcagttaa ccaaatcgtt aaacgcatga ataatggtga
     3961 aggaatttaa ggaggaaagt atgaaaaatt cgattatgga tacaaaattt gatagacgta
     4021 tcttactctt aaataaaatc attattgtct ttatcgtttt gatgactttg cttcctttac
     4081 tttatatcgt cgtagcatcc tttatggatc ctaaggttct ggttagtaga gggattagct
     4141 ttaatccagc cgattggact gtagaaggtt accagcgtgt attcagtgac caatctattc
     4201 taagaggttt tatcaattct ctactatact cttttggatt tgcagcttta acagtattgc
     4261 tatctgtgtt tacagcttat cctctttcta agaaagactt ggttggacgt cgttggatta
     4321 actacttctt gattgtgact atgttctttg gtggtggttt agtcccaact tacttgctcg
     4381 taaaagaatt gggaatgctc aatactccat gggctatcat tgttccaggt gctgtcaatg
     4441 tttggaatat tattcttgct agggcctatt tccaaggatt gcctgaagaa ttagttgaag
     4501 ctgctgtcat tgatggagcg aatgacttgc agattttctt taaaatcatg cttcctcttg
     4561 caaaaccaat tatgtttgtt ctcttcctct atgcttttgt aggacagtgg aactcatact
     4621 ttgatgcaat gatttatatc aaggatccaa acttggaacc attgcaactt gtacttcgta
     4681 aaattctcat tcagagccaa ccaggtcaag acatgattgg agcacaagcg gctatgaatg
     4741 aaatgaaacg tttagctgaa ttgattaaat acgcaactat tgtcatttcc agcttgccat
     4801 tgattgttat gtatccattc ttccaaaaat actttgataa aggaattatg gctggttcac
     4861 ttaaaggata aaaaaagaaa aaataaaagg agttttctca tgaaattcaa aacattctca
     4921 aaatcagcag ttttgttgac agctagttta gcagtacttg cagcctgtgg ctcaaaaaat
     4981 acagcttcaa gtccagatta taagttggaa ggtgtaacat tcccgcttca agaaaagaaa
     5041 acattgaagt ttatgacagc cagttcaccg ttatctccta aagacccaaa tgaaaagtta
     5101 attttgcaac gtttggagaa ggaaactggc gttcatattg actggaccaa ctaccaatcc
     5161 gactttgcag aaaaacgtaa cttggatatt tctagtggtg atttaccaga tgctatccac
     5221 aacgacggag cttcagatgt ggacttgatg aactgggcta aaaaaggtgt tattattcca
     5281 gttgaaaatt tgattgataa atacatgcca aatcttaaga aaattttgga tgagaaacca
     5341 gagtacaagg ccttgatgac agcacctgat gggcacattt actcattccc atggattgaa
     5401 gagcttggag atggtaaaga gtctattcac agcgtcaacg atatggcttg gattaacaaa
     5461 gattggctta agaaacttgg tcttgaaatg ccaaaaacta ctgatgattt gattaaagtc
     5521 ctagaagctt tcaaaaacgg ggatccaaat ggaaatggag aggctgatga aattccattt
     5581 tcatttatta gtggtaacgg aaacgaagat tttaaattcc tatttgctgc atttggtata
     5641 ggggataacg atgatcattt agtagtagga aatgatggca aagttgattt cacagcagat
     5701 aacgataact ataaagaagg tgtcaaattt atccgtcaat tgcaagaaaa aggcttgatt
     5761 gataaagaag ctttcgaaca tgattggaat agttacattg ctaaaggtca tgatcagaaa
     5821 tttggtgttt actttacatg ggataagaat aatgttactg gaagtaacga aagttatgat
     5881 gttttaccag tacttgctgg accaagtggt caaaaacacg tagctcgtac aaacggtatg
     5941 ggatttgcac gtgacaagat ggttattacc agtgtaaaca aaaacctaga attgacagct
     6001 aaatggattg atgcacaata cgctccactc caatctgtgc aaaataactg gggaacttac
     6061 ggagatgaca aacaacaaaa catttttgaa ttggatcaag cgtcaaatag tctaaaacac
     6121 ttaccactaa acggaactgc accagcagaa cttcgtcaaa agactgaagt aggaggacca
     6181 ctagctattc tagattcata ctatggtaaa gtaacaacca tgcctgatga tgccaaatgg
     6241 cgtttggatc ttatcaaaga atattatgtt ccttacatga gcaatgacaa taactatcca
     6301 agagtcttta tgacacagga agatttggac aagattgccc atatcgaagc agatatgaat
     6361 gactatatct accgtaaacg tgctgaatgg attgtaaatg gcaatattga tactgagtgg
     6421 gatgattaca agaaagaact tgaaaaatac ggactttctg attacctcgc tattaaacaa
     6481 aaatactacg atcaatacca agcaaacaaa aactagaggt tgattatggg agataagaaa
     6541 tacacagtag aaaaagccaa tcgttttata gcagaaaata aacatctcgt taatactcaa
     6601 tataagcctg aagaacattt ttcagctgag attggttgga tcaatgatcc aaatggattt
     6661 gtctattttc gtggagaata ccatctcttt tatcaattct atccatatga tagtgtttgg
     6721 gggcctatgc actggggaca tgctaaaagt aaggacttgg tgacttggga gcacttgcca
     6781 gtggcacttg ctcctgacca agattatgac cgaaatggtt gtttctcagg ctctgccatt
     6841 gtcaaggatg atcgcctctg gctcatgtac actggacata tcgaagaaga aaccggtgtc
     6901 cgccaagtgc aaaatatggc attttcagat gacgggattc actttgaaaa gattgaacaa
     6961 aatccagttg caactggatc agacttgcca gatgagttga ttgctgctga ttttcgtgat
     7021 ccaaaactct ttgaaaaaga tggacgctat tactccgtag tagctgccaa acacaagaat
     7081 aatgtgggct gtatcgttct actagggtcc gataacctag tagaatggca gttcgaatcc
     7141 atctttttaa aaggggtaga acaccaaggc tttatgtggg aatgcccaga ttacttcgag
     7201 ttagatggga aagattgcct tattatgtca cccatgcgtt atcagcgtga gggagactca
     7261 tatcataaca tcaactcatc gcttttgttc acgggtaagg tagattgggg agaaaaatgt
     7321 tttatcccag aatcagttca agaaattgat catggccaag acttctatgc gcctcaaaca
     7381 ttgttggacg atcaaaatcg tcgtatcctg attgcttgga tgcagacatg ggggcgtacc
     7441 cttccaaccc atgaccaaga acacaaatgg gcatgtgcca tgactttacc tagaatttta
     7501 agattggaag atggcaaact aagacaattc cctgttaaaa aaggccaata tcaaatccaa
     7561 atagataaag attgtcatta ccacttagga aatgatatag attatcttga atttggttat
     7621 gatagtaatg cgcagcaagt ttacattgat cgtagccatc ttattcaaaa aattctaggt
     7681 gaagaagaac aggacactag tcgacggtat gtagatattg aagctaaaga attggaagtt
     7741 gttctagata aaaattccat cgagattttt gtcaatcaag gtgaagcaag cttgactgca
     7801 acttattact taacggtgcc agctgagcta tcacgaattg attaaaaatt aagttatttc
     7861 tcctaaagaa aaagttctct ttctaaaata gtggaaagag gactttttgt gttttgggta
     7921 tataagctta gtttatggta tttgtaaaat tggtgttgga ttatgattta agctagtttt
     7981 ctaaagaatt tgaaaaaaat tttatttaag caaaaaaaac ccttggttcc aaggctttt
//
LOCUS       DAVLVF010000082         7999 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVLVF010000082 DAVLVF010000000
VERSION     DAVLVF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7999
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.082"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(164..304)
                     /locus_tag="VYN66_001680"
     CDS             complement(164..304)
                     /locus_tag="VYN66_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blpT protein"
                     /protein_id="HEV1495671.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQVTADDIRLQELLNTTMQELKKQ
                     KS"
     gene            473..1015
                     /locus_tag="VYN66_001681"
     CDS             473..1015
                     /locus_tag="VYN66_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV1495672.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            1015..1644
                     /gene="nth"
                     /locus_tag="VYN66_001682"
     CDS             1015..1644
                     /gene="nth"
                     /locus_tag="VYN66_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV1495673.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            1855..2376
                     /gene="pyrR"
                     /locus_tag="VYN66_001683"
     CDS             1855..2376
                     /gene="pyrR"
                     /locus_tag="VYN66_001683"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV1495674.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            2395..3318
                     /locus_tag="VYN66_001684"
     CDS             2395..3318
                     /locus_tag="VYN66_001684"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV1495675.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            3368..4447
                     /gene="carA"
                     /locus_tag="VYN66_001685"
     CDS             3368..4447
                     /gene="carA"
                     /locus_tag="VYN66_001685"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV1495676.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            4759..7935
                     /gene="carB"
                     /locus_tag="VYN66_001686"
     CDS             4759..7935
                     /gene="carB"
                     /locus_tag="VYN66_001686"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV1495677.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         2322 a         1617 c         1802 g         2258 t
ORIGIN      
        1 acaggtccat agagacttag tacgtgtttg acatactcgt aatggatttt atcatagttt
       61 cgccaagcag tgcgtgcttg attgtttagt tttaggctac ccagtccaat cagaggactg
      121 gtgggttggt aaaatttctc aagattaatt aagatactgt tgttcaactt ttctgttttt
      181 tgagctcttg catagtagtg tttagcagct cttgtagtcg aatatcatct gccgtaacct
      241 gtttacagtt ttggtagctt ttatttaact tggataggag ttgaatgagt ttcttgtctt
      301 ccatgtgagc atcctccttg atctgatgta ctctttccct atagtatagc actaaaaaat
      361 aaaactatca tagtaaatat gttaaaattt gaattttaat tacaaagttt ggtgtgctga
      421 tttgtagcct tttcatggta taatcaagag agtaaatctt tatggaggaa gtatgaaatt
      481 aaatattcaa gaaattcgta agcagtctga aggtttgaac tttgaacaaa cgttagattt
      541 agttgatgac ctgcgtgcac gtaatcaaga aattttagat gtaaaagata tccttgcagt
      601 tgggaaagta caatatgaag accgtatgta tttcttagat tatcaactat cttataccat
      661 tgttcttgct tcgagtcgca gtatggagcc agttgagtta gttgaatctt atccagtcac
      721 ggaagttttc atggaaggcg caactaacca gctagatcaa gaagttttag atgatgactt
      781 ggtcttgccc atcgaaaatg gggcgcttga ccttgctgag agtgtatcag acaatatcct
      841 gctaaacatt cctatcaagg tcttgacggc tgaagaagaa gctggtcaag gatttgtctc
      901 aggaaatgac tggcaaatca tgacagagga agaataccaa gctcaacaag cagtaaagaa
      961 agaagaaaac agtccttttg ctggcttaca aggactattt gacggagatg aataatggtc
     1021 ttgtcaaaaa aacgagcacg aaaggtgcta gaagaaatca ttgccctctt cccagatgcc
     1081 aagcctagtc ttgattttac caatcatttt gaactcctgg ttgcggtcat gttatcagcc
     1141 cagacgacag atgcagcggt aaataaggcc acaccaggtc tctttgttgc ctttccaaca
     1201 ccccaagcta tgtctgtagc gacagagagt gagattgctt cacatatttc tcgcttggga
     1261 ttgtatcgga ataaagctaa attccttaaa aaatgtgccc aacagttact agacgatttt
     1321 gatggtcaag tccctcagac acgtgaagaa ttggagagtt tggcaggtgt tggtcgcaag
     1381 acagccaatg ttgtcatgag tgtaggattt gggattccag cctttgcagt ggatactcat
     1441 gtggagcgta tttgcaaaca ccacgatatt gtcaaaaaat cagcgacgcc acttgaggtg
     1501 gaaaagcggg tcatggatat cttgccgcct gagcagtggt tagctgccca tcaggccatg
     1561 atttattttg gaagagccat ttgccatcca aaaaatccag agtgtgacca gtacccacaa
     1621 ttatatgatt ttagcaattt gtaagatgct acattgaaac tttttgcttg attttaagca
     1681 tgctttgtgc tatagtaaat gataactaaa tattccttta aacctgtccc gtgaggcagg
     1741 caaggagcgc gataaagtag aagggtgaag tctacactgt gtgcagtagg gctcgcttgg
     1801 ctataaattt caagtctcct tgtttaagga gacttttctt tttggaggtt tgtcatgaaa
     1861 acaaaagaag ttgtagacga attgaccgtc aaacgagcga ttacgcgtat tacttatgag
     1921 attatcgaac gcaacaaaga tttgaataag atcgtcttgg ctggtattaa aactcgtggt
     1981 gtctttattg cccaccgaat ccaagaacgt ttgaagcagt tagaaaatct ttcagttcct
     2041 gttgtggaat tggatactaa acctttccgt gatgatgtta aaagtggaga agatacttct
     2101 ttggtttctg tcgatgtgac agaccgcgaa gttatcttgg tggatgatgt gctctataca
     2161 ggtcgtacca tccgtgctgc tattgataat attgtcggtc atggtcgtcc tgcgcgtgtg
     2221 agtttagcag ttctagtcga tcgtggacat agagaattgc caatccgtcc agattacgtt
     2281 ggaaaaaata tcccaaccag tcgttctgaa gaaatcatcg tagagatgac agaacttgat
     2341 gaccaagaca gagttctgat tactgaagaa gcttagaaag ttaaaggagt agccatgtca
     2401 gaaaatcaac aagcattgaa ccatgtggtg tccatggaag acctcactgt cgatcaagta
     2461 atgaaattga tcaagcgagg aattgagttt aaaaatggag cccagcttcc ctatgaagac
     2521 catccgattg tttccaatct cttctttgag gattctacac ggacacataa gtcctttgaa
     2581 gtcgcagaga ttaaacttgg attggaacga cttgactttg atgtgaagac tagttcggtt
     2641 aataagggtg agacacttta tgacaccatt ttgactctgt ctgctttagg agtggatgtc
     2701 tgtgtgattc gccatccaga ggtcgactac tatagagaat tgattgctag tccaacgatt
     2761 acgacttcca tcatcaatgg tggagatggt tcgggccaac accctagcca gagcttgctt
     2821 gatttgatga ccatttatga ggaatttggc cactttgagg gtcttaaagt tgctattgca
     2881 ggtgacttgg atcactcacg cgttgccaaa tccaatatgc agattttgaa acgcttggga
     2941 gctgaactct ttttcgctgg acctgaggaa tggagaagtc aagagtttgc agactatgga
     3001 cagtttgtaa ccattgatga aatcattgat caggttgatg tcatgatgtt tctccgtgtg
     3061 caacacgaac gccatgatag tggagctgta ttttcaaaag aagactacca tgcccaacat
     3121 ggcttgactc aagaacgtta cgatcgtttg aaagaaacag caatcctcat gcacccagct
     3181 ccaatcaatc gtgatgtaga aatagcagac cacttggttg aagcaccaaa atcacggatt
     3241 gtccaacaaa tgaccaatgg tgtctttgtt cgaatggcaa tcttagaatc cgtactagcg
     3301 agtagaaacg ccaactaaaa cacaagacgt taaaagacgc cagtgagcgt ccacgagagg
     3361 tgaaaatatg acaaaaagaa ttctagtatt agaagatggc acagtttttg aaggcaaggc
     3421 cttcggagca gatattgatg taacaggcga aatcgtcttt aatacaggga tgaccggcta
     3481 ccaagaatcc attacagacc agtcttataa tggacaaatc ttgaccttta cttatccttt
     3541 ggtaggaaat tatggtatta atcgtgatga ttatgaatcc attattccaa cttgtaaggg
     3601 agtcgttgtt tttgaagaag cgcgtagagc tagcaactgg cgcaaccaaa tgaccttgga
     3661 tgaatttttg aaagccaaga aaattccagg gatttcagga attgatacgc gtgctcttac
     3721 caagattatc cgtaagcatg gtactatgcg tgcaaccttg acccatgttg gggacagtat
     3781 ggaccatgtg acggaccagc tccaagcaac agttttaccg acagacaata tcaaacaggt
     3841 ttctactaaa acgtcttatc cagctccagg agttggtttg agcgtggtgc tagtggactt
     3901 tggtctcaag cactcaatcc tacgtgaact ttctaaacgc aactgtaacg tgacagttgt
     3961 tccttattcg acaacggctg aagaaattct ccatctcaat cctgatggag ttatgttgtc
     4021 aaatggtcca ggtaacccag aagacgttcc acaagcactc gacatgattc gcggtgtaca
     4081 aggaaaaatt ccaatctttg gtatttgtat gggacaccaa ctctttgcaa tggcaaacgg
     4141 ggctaagacc tataagatga agtttggcca tcgtggattc aaccatgcgg tacgtgaaat
     4201 cgcaacagga cgagtagact tcaccagcca gaaccatggt tatgcagtca gccgcgagga
     4261 tttgccagag cacttgatta ttacccacga agaaatcaat gacaagtcag tggaaggtgt
     4321 gcgccacaga taccaacctg ctttctctgt tcaataccac ccagatgcag ctcctgggcc
     4381 acacgacgct agctacctat ttgacgagtt tatcgagatg atggaagttt ttaaacaatc
     4441 aaactaagaa cgagtaaaag ctcgtcggag aatttatgta actgaacacg gacggaaagc
     4501 tcagaattta gagaaaccaa cttggattgt caatccttcc ttgatttctt aaaattcaat
     4561 cgctttctat tcgtcctttg tatcttattt aatgtcgccc tgagaggcga cgaatagaaa
     4621 ggagaagata cattgttcgc ggaagtgaac acgccctaag aactgtgtga aaaagataaa
     4681 catcgtcttg acgctgaagg cgtctgcgcc gagtttccta ttttcactgt gttctttacg
     4741 ggctttgtat cataaactat gcctaaacgt actgatattc aaaaaattat ggtgattggt
     4801 tctggtccga ttattattgg tcaggctgct gagtttgact acgctgggac ccaggcttgc
     4861 ttgtcgttga aagaggaagg ttatgaggtt gttttggtta actcaaatcc tgcaaccatc
     4921 atgacggaca aggaaattgc tgacaaggtt tacattgaac cgattacact tgagtttgtg
     4981 acacgtattc ttcgtaagga acgtccagat gccttgctac caacactcgg tggtcagaca
     5041 ggtctcaaca tggccatgga attatctaaa aatggtatcc tagacgagct tggcgttgaa
     5101 cttctgggta ccaaattatc tgccattgac caagcggagg accgtgacct ctttaaacaa
     5161 ttgatggaag agcttgagca acccattcca gaatctgaaa ttgttaacac agtagaagaa
     5221 gcagttgcct ttgcagcgac aattggctac ccagtcatcg ttcgtccagc ctttaccctt
     5281 ggtggtactg gtggtggtat gtgtgccaac gaaaaagaat tgcgtgaaat cactgaaaat
     5341 ggtttgaaat tgtcacctgt tacccaatgt ttgattgagc gttcgattgc cggttttaag
     5401 gaaattgaat acgaagtgat gcgtgactca gctgacaatg ccttggttgt ttgtaacatg
     5461 gaaaactttg acccagttgg gattcacaca ggggattcca ttgtatttgc ccctgcgcaa
     5521 accatgtcag actatgaaaa ccaaatgctt cgtgatgcga gcttgagcat tattcgcgcc
     5581 ctcaagattg aaggtggatg taatgttcag ctagctcttg atcctaacag cttcaagtat
     5641 tatgttatcg aagtaaaccc tcgtgtatca cgttcgtcag cccttgcttc taaagcgaca
     5701 ggatatccga ttgctaagtt ggctgccaag attgcagtag gtttgacctt ggatgaggtt
     5761 ataaacccag ttacaggttc aacctatgcc atgtttgaac cagcccttga ctacgtagtt
     5821 gctaagattc cacgtttccc atttgacaag tttgaaaagg gtgaacgccg tctcggtaca
     5881 caaatgaagg cgactggaga agtcatggca atcggtcgta acatcgagga atctcttctc
     5941 aaggcctgcc gctcccttga aattggggtg caccacaatg aaatacctga acttgcagca
     6001 gtttctgatg atgccttgat tgaaaaggtt gtcaaagccc aagatgaccg tctcttctac
     6061 gtatcagaag ccattcgtcg tggctacaca cctgaagaaa ttgcagagct tacaaaaatt
     6121 gatatcttct atcttgataa actcttgcat atctttgaaa ttgagcaaga attgggtgct
     6181 catccacaag atctagaagt tttgaaaaca gccaaactta atggtttctc agaccgtaag
     6241 attgctgaac tctggggaac gactgatgat caagttcgcc aacttcgctt ggaaaacaag
     6301 attgttccag tttataagat ggtagatact tgtgcggcag agttcgactc tgaaacgcca
     6361 tatttctatt caacctatgg ttgggaaaat gaatctatca gatctgataa agaatctgta
     6421 cttgtcctag gttcaggtcc aatccgtatc ggtcaagggg ttgagtttga ctacgcaacc
     6481 gttcactctg ttaaggctat ccaggcagct ggttatgaag ctatcatcat gaactcaaac
     6541 ccagagaccg tttcaacaga cttctctgta tcagataagc tttactttga gccattgaca
     6601 ttcgaagatg ttatgaacgt cattgacttg gaacaaccaa agggagttat cgttcagttt
     6661 ggtggtcaaa cagccatcaa ccttgcggag ccattggcaa aagcaggtgt gaccatcctt
     6721 ggtacacagg ttgctgacct agaccgtgcc gaagaccgtg acctcttcga gcaagctctc
     6781 aaagaattgg atattccaca gccaccagga caaacggcta ccaatgaaga agaagcagct
     6841 cttgcagctc gtaagattgg cttcccagtt cttgtccgcc catcttatgt acttggtgga
     6901 cgtgccatgg aaatcgttga aaacgaagaa gaccttcgtt cttacatgcg aactgctgtt
     6961 aaggctagtc cagatcaccc agttcttgtc gactcttata tcgttgggca agagtgcgaa
     7021 gttgatgcca tttcagatgg taaagatgtg ctcattcctg gtatcatgga gcatatcgaa
     7081 cgtgctggtg tccactcagg tgactcaatg gctgtttacc caccacaaac cttgtcgcaa
     7141 aaggttcagg agaccatcgc agactacacc aaacgcttag caatcggtct tcactgcctt
     7201 ggaatgatga acatccagtt tgttatcaag gatgaaaaag tctacgttat tgaggtcaat
     7261 ccacgtgcca gccgtactgt tccattccta tctaaggtaa ccaatattcc tatggctcaa
     7321 gtagcgacca agctcattct tggtcaaagt ttatcagaac ttggctacca aaatggactt
     7381 taccctgaaa gcacccgtgt tcatatcaag gcacctgtct tctcctttac caaactagct
     7441 aaggtagaca gcttgctcgg tcctgaaatg aagtcaacag gtgaagttat gggttctgat
     7501 gctactttgg aaaaagctct ctataaagcc tttgaagctt cttatctaca cttgccaact
     7561 tttggtaatg tcgtctttac catcgctgat gatgccaaag aagaagcctt gaacttggct
     7621 cgtcgtttcc aaaacattgg ttatggaatc cttgcgacag aagggacagc agccttcttt
     7681 gctagtcatg gcttgcaagc ccaacctgtt ggtaagattg gtgatgacga taaggatatc
     7741 ccaagttttg tacgcaaagg aagaattcaa gctatcatta acacagtagg aacaaaacga
     7801 actgctgacg aagatggtga gcaaattcgt cgttcagcca ttgaacacgg ggtgcccctc
     7861 ttcacagcgc tagatacagc taatgccatg cttaaggtac ttgaaagtcg tagctttgtt
     7921 acagaagcga tttaaataga aaaataagat atagcagact gaatttagaa cagtacacag
     7981 gaatactaaa atatttcta
//
LOCUS       DAVLVF010000083         7680 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVLVF010000083 DAVLVF010000000
VERSION     DAVLVF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.083"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            63..605
                     /locus_tag="VYN66_001687"
     CDS             63..605
                     /locus_tag="VYN66_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1495678.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDVIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSIDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(646..719)
                     /locus_tag="VYN66_001688"
     tRNA            complement(646..719)
                     /locus_tag="VYN66_001688"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:Asn,seq:gtt)
     gene            complement(725..796)
                     /locus_tag="VYN66_001689"
     tRNA            complement(725..796)
                     /locus_tag="VYN66_001689"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(761..763),aa:Glu,seq:ttc)
     gene            complement(848..1600)
                     /locus_tag="VYN66_001690"
     CDS             complement(848..1600)
                     /locus_tag="VYN66_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1495679.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(1597..2922)
                     /locus_tag="VYN66_001691"
     CDS             complement(1597..2922)
                     /locus_tag="VYN66_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1495680.1"
                     /translation="MDLLGFGTVIVHFLIISHSYRLICKGRINRKELYIFGAYTLLTE
                     IVLEFSFYLLYLDKIGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     FKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVMVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(2943..3068)
                     /gene="comC"
                     /locus_tag="VYN66_001692"
     CDS             complement(2943..3068)
                     /gene="comC"
                     /locus_tag="VYN66_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV1495681.1"
                     /translation="MKNTVKLEQFVALKEKDLQNIKGGEMRISRIILDFLFLRKK"
     gene            complement(3233..3306)
                     /locus_tag="VYN66_001693"
     tRNA            complement(3233..3306)
                     /locus_tag="VYN66_001693"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3270..3272),aa:Arg,seq:tct)
     gene            complement(3350..3829)
                     /gene="rlmH"
                     /locus_tag="VYN66_001694"
     CDS             complement(3350..3829)
                     /gene="rlmH"
                     /locus_tag="VYN66_001694"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV1495682.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            4012..5193
                     /locus_tag="VYN66_001695"
     CDS             4012..5193
                     /locus_tag="VYN66_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1495683.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            5251..6009
                     /locus_tag="VYN66_001696"
     CDS             5251..6009
                     /locus_tag="VYN66_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1495684.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLETLLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            6222..7583
                     /gene="dnaA"
                     /locus_tag="VYN66_001697"
     CDS             6222..7583
                     /gene="dnaA"
                     /locus_tag="VYN66_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV1495685.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         2675 a         1380 c         1216 g         2409 t
ORIGIN      
        1 aaattacaag ataaaaaatc tatttttaga catatagtct aatttgttta caaggaggaa
       61 atatgcaaga aagtaacaaa cgcttaaaaa caaagcgaac tattgaaaat gctatggtac
      121 aattactgat ggaacagcca tttgataaaa tttctactgt caaattagta gaaaaagccg
      181 gaattagtcg ttccagcttc tatactcact ataaggataa gtatgatgtg attgagcact
      241 atcaaagcaa gctattccat acatttgaat atatttttca aaaacatgct catcacaaaa
      301 gagacgctat tttagaagtc tttgaatatc tagagtcaga accacttctg gctgcccttc
      361 tttctgaaaa tgggactaaa gaaatccaaa atttcttacg aaataaactt catatcatgc
      421 tcagtataga tttacaaaag cgatttatgc aactgaatct aaataccact gaattagaat
      481 atagtagcat ctatctaact catgcacttt ttggtgtctg ccaaacttgg attgcacatg
      541 gaaaaaaaga aagttctcaa gaaataacag acttccttat gaaaatgctt ggtgatacaa
      601 attgatacaa aaagaggaac acagctgtgt tcccttttat ctatactccg ccagtaggac
      661 tcgaacctac gacatcatga ttaacagtca tgcgctacta ccaactgagc tatggcggat
      721 aaaatagtcc gtacgggatt cgaacccgtg ttaccgccgt gaaaaggcgg tgtcttaacc
      781 ccttgaccaa cggaccttct atctgtagca gatataacca ttatatcaat ttcttgctaa
      841 ttgtcaatca cttttgagat tttttctcta aaatatcttt taattttcta atttttaatc
      901 ttgaaatagg acaacgatgg tcttcataga aaacaatttc taagtttttt cgatcaattt
      961 ctctgatatt gcctatattt accaaaaatg acttgtgagg agaataaaat cgctgagtat
     1021 gtttgtcctt ttcctgaata tctgtcatgg taccataaaa ctcttttgca aaattcttac
     1081 caataatgcg caatttatga gatacccctg ttgtttcaat atacaaaata tcatggtaag
     1141 gaatttttaa atcatttccc ttgtaattgt agtcgaaata atctacaaca tcttcatttt
     1201 caagtaacat actcttcgtg tagaagatat tttgctcaat tctcttctta aacatctcat
     1261 cattgatatc cttatcaaca aaatctaggg ctgatacctg gtatttatag gttagagtcg
     1321 caaactctga tcgactagtg ataaagacaa taatagcgta aggattgtaa tgacgaatga
     1381 gctgagccac ttcaaatccc tttttctcaa ttccatgaat atcgatatct aggaaataaa
     1441 gctgatttac ttcatcattt tcaatgtatt cttcaaattc acggactttt cccgttgtct
     1501 tgtatgatat tggaatattc gattctttcg aaatttcatc caatattctc tctagtctca
     1561 cttgatgttc aataacatct tctaaaatta aaactttcat tcaaattccc tcttaaatct
     1621 aatgatttgt ctaaatgtac tgccttccat ctctgtttct aaaataatat tgttgtactt
     1681 atctagtagt tctttcacat tatttaatcc gactccgcga tttcttccct tagtggagaa
     1741 tcctaaggca aatagatctc ctgaaggagt catcgtcatt ttacatgaat tctgaatcac
     1801 cataactgtt tcagtttcca tcttaataac tgctacttcc atctgctttt tatagctatc
     1861 agccgatcct tcgacagcat tattcaataa aacgctcatg atacgaacca aatccaatag
     1921 ttcaattgga agcttggtaa tcgtatcttt tacttccagt gtaaactcta caccattatt
     1981 tcgagcatag acaattgact gagcaaccaa acttcgtaaa gctgagtctt ctatgttgtt
     2041 caaatcaaag taagtgtact tatctgaacg caatttatga tttgctttga ctaaaacttc
     2101 attgtaaatt ctgtcaattt cctgtaaatt accactgtca attgccatct gcatgctgac
     2161 aagcattcca gcataatcat gtcgaaaacc acggatttca ttatacagac caacaatttc
     2221 atctgtgtaa ttctgtaaat gtttctgttc aaatttcttc tgcttaaaag caatctcttt
     2281 ctccatttga actttatgag aattcattgc aaagaaggtc aaaaggagag agataaagac
     2341 aatagatgac aaaatacttc caaaactatt caaatgttta atcgtactta ccatatctga
     2401 aacgaaagat acaatatgta gcaatagtaa agcaaaaaat acttttttca agaaaggata
     2461 aaggtagtcc ttgtcaaaat aggctagttc caaatggaaa taataaatga tttttaatgt
     2521 aacaaaatag gttaacaccg tcacaacgaa aaagaatggg aaatgatatt gtaaaacaaa
     2581 attatctcct gttatagagg agaaagttac ggacagaaag ttatgagtgc tctcatataa
     2641 aagagatagt agtaaactta ggaatagtcc tctatccctc tcatactgtt tcatccatcg
     2701 aaaataggaa tataagccca aaggaaataa aaatctttca atccctattt tatctaaata
     2761 tagaagataa aaggaaaatt caagtactat ttcagttagt aatgtataag caccaaaaat
     2821 gtataattct tttctattta ttcgaccttt acaaattaaa cggtaactgt gactaataat
     2881 taaaaaatga acaataactg tcccaaatcc aagtaaatcc attactcttt ctccttattt
     2941 cattactttt ttcgtaggaa aagaaaatca aggatgattc ttgaaatcct catctcccca
     3001 cctttaatat tttgtaagtc tttttccttc aaagctacaa actgttccaa tttaactgtg
     3061 tttttcataa taaaatctcc taaaatgttt tttcttgtaa gctaacttac aaaaaccatt
     3121 atacaaaatg gaatttcact ttagataaaa ttctctcaac tgtcattttt ttctcccaaa
     3181 gtgtactttt ttaagaaaaa agccgggaaa attcccagct ttgctattat attgatccca
     3241 gcaggattcg aacctgcgac cgttcgctta gaaggcgaat gctctatcca gctgagctat
     3301 gagacctaac acaattattc taccaaaaat tcaattaaaa gtcaattttc tatttatggt
     3361 aggggaatcc ctgctgaatc gtaaaagcgc gatagatttg ttcaacaaga actagtctca
     3421 ttaactgatg aggcaaggtt aggcgaccaa aactgacaga aagattggct ctatttttta
     3481 cagatgatga caatcctaaa cttcccccaa taataaaagt aagagtagaa aatcctttta
     3541 tagaagtttc ttctaactgc ttactaaatt cttctgagaa gaaagttttc ccttcaatgg
     3601 ctaacacaat aacgaaatca cggtcagcaa tttttgataa aattctctga ccttctattt
     3661 ctaaaatctt ttgattttct gattcactgg ccttatctgg tgttttttca tctgataact
     3721 caatcatttc aaacttagca aatctagaaa ttcgttttga atactctgcg ataccatctt
     3781 ttaaatactt ttctttcagt ttcccaactg ttacaacttt aattttcatg actctattct
     3841 aacatattct ctattttttc acatcttatt cacaaaataa aaaatagatt tcaattaaga
     3901 aaatcacagt ttcaaaagag ttatccacag tttgtgtaaa acttttgtgt ttaagttata
     3961 attaagctag tcagtttata ctttcagtaa ttcaaacata tggaggcaaa tatgaaacat
     4021 ctaaaaacat tttacaaaaa atggtttcaa ttattagtcg ttatcgtcat tagctttttt
     4081 agtggagcct tgggtagttt ttcaataact caactaactc aaaaaagtag tgtaaacaac
     4141 tctaacaata atagtactat tacacaaact gcctataaga acgaaaattc aacaacacag
     4201 gctgttaaca aagtaaaaga tgctgttgtt tctgttatta cttattcggc aaacagacaa
     4261 aatagcgtat ttggcaatga tgatactgac acagattctc agcgaatctc tagtgaagga
     4321 tctggagtta tttataaaaa gaatgataaa gaagcttaca tcgtcaccaa caatcacgtt
     4381 ataaatggcg ccagcaaagt agatattcga ttgtcagatg ggactaaagt acctggagaa
     4441 attgtcggag ctgacacttt ctctgatatt gctgtcgtaa aaatctcttc agaaaaagtg
     4501 acaacagtag ctgagtttgg tgattctagt aagttaactg taggagaaac tgctattgcc
     4561 atcggtagcc cgttaggttc tgaatatgca aatactgtca ctcaaggtat cgtatccagt
     4621 ctcaatagaa atgtatcctt aaaatcggaa gatggacaag ctatttctac aaaagccatc
     4681 caaactgata ctgctattaa cccaggtaac tctggcggcc cactgatcaa tattcaaggg
     4741 caggttatcg gaattacctc aagtaaaatt gctacaaatg gaggaacatc tgtagaaggt
     4801 cttggtttcg caattcctgc aaatgatgct atcaatatta ttgaacagtt agaaaaaaac
     4861 ggaaaagtga cgcgtccagc tttgggaatc cagatggtta atttatctaa tgtgagtaca
     4921 agcgacatca gaagactcaa tattccaagt aatgttacat ctggtgtagt tgttcgttcg
     4981 gtacaaagta atatgcctgc caatggtcac cttgaaaaat acgatgtaat tacaaaagta
     5041 gatgacaaag agattgcttc atcaacagac ttacaaagtg ctctttacaa ccattctatc
     5101 ggagacacca ttaagataac ctactatcgt aacgggaaag aagaaactac ctctatcaaa
     5161 cttaacaaga gttcaggtga tttagaatct taattgacat ctatgtaaag aaagctttac
     5221 ataagagaaa agatgtgtta gtgtagaatc atggaaaaat ttgaaatgat ttctatcaca
     5281 gatatacaaa aaaatcccta tcaaccccga aaagaatttg atagagaaaa gctagatgaa
     5341 ctagcacagt ctatcaaaga aaatggggtc attcaaccga ttattgttcg tcaatctcct
     5401 gttattggtt atgaaatcct tgcaggagag agacgctatc gggcttcact tttagctggt
     5461 ctacggtcta tcccagctgt tgttaaacag atttcagacc aagagatgat ggtccagtcc
     5521 attattgaaa atttacagag agaaaattta aacccaatag aagaagcacg cgcctatgaa
     5581 tctctcgtag agaaaggatt cacccatgct gaaattgcag ataagatggg caagtctcgt
     5641 ccatatatca gcaactccat tcgtttactt tccttgccag aacagattct ctcagaagta
     5701 gaaaatggca aactatcaca agcccatgcg cgttccctag ttgggttaaa taaggaacaa
     5761 caagactatt tctttcaacg gattatagaa gaagatattt ctgtaaggaa gttagaaact
     5821 cttctgacag agaaaaaaca aaagaaacag caaaaaacta atcatttcat acaaaatgaa
     5881 gaaaaacagt taagaaaact actcggatta gatgtagaaa ttaaactatc taaaaaagac
     5941 agtggaaaaa tcattatttc tttttcaaat caagaagaat atagtagaat tatcaacagc
     6001 ctgaaataag gctgttcttt tattttttta tctcacaagg ttatccacta tgtttttcga
     6061 taaaaagctt aataaatcaa taatttcttc ttttatcccc aacctgtgga taaagtttgg
     6121 taacattgtg gattattttt cacagcttgt ggaaaattct tgctatctat ggtaaaatat
     6181 ctctagtatt aaacttttaa atagtaaagg aggagaaagg attgaaagaa aaacaatttt
     6241 ggaatcgtat attagaattt gcacaagaaa gactgactcg atccatgtat gatttctatg
     6301 ctattcaagc tgaactcatc aaggtagagg aaaatgttgc cactatattt ctacctcgct
     6361 ctgaaatgga aatggtctgg gaaaaacaac taaaagatat tattgtagta gctggttttg
     6421 aaatttatga cgctgaaata actccccact atattttcac caaacctcaa gatacgacta
     6481 gctcacaagt tgaagaagct acaaatttaa ctctttatga ctatagtcca aagttagtat
     6541 ctattcctta ttcagatacg ggattaaaag aaaagtatac ctttgataac tttattcaag
     6601 gggatggaaa tgtttgggct gtatcagccg ctttagctgt ctctgaagat ttggctctga
     6661 cctataaccc tctttttatc tatggaggac caggccttgg taagactcac ttattaaacg
     6721 ctattggaaa tgaaattcta aaaaatattc ctaatgcgcg tgttaaatat atccctgccg
     6781 aaagctttat taatgacttt cttgatcacc taagacttgg ggaaatggaa aagtttaaaa
     6841 agacctatcg tagtcttgat cttttgttaa tcgatgatat acagtcactc agcggaaaaa
     6901 aagtcgcaac tcaggaagaa tttttcaata cctttaacgc ccttcatgac aagcaaaaac
     6961 agattgtcct aacgagtgat cgtagtccaa aacatctaga agggctcgag gagaggcttg
     7021 tcacgcgttt tagttgggga ttgacacaaa ctatcacacc ccctgacttt gaaacacgta
     7081 ttgccatttt acaaagtaag acggaacatt taggctacaa tttccaaagt gatactctag
     7141 aatacctagc tgggcaattt gattcaaatg ttcgagatct tgagggagcc atcaacgaca
     7201 tcactttaat tgccagagta aaaaaaatca aggatatcac tattgatatt gctgcagaag
     7261 ccattagagc ccgcaaacaa gatgttagcc aaatgctcgt catcccaatt gataaaatcc
     7321 aaactgaagt tggtaacttt tatggtgtta gtatcaaaga aatgaaggga agtagacgcc
     7381 ttcaaaatat tgttttggcc cgtcaagtag ccatgtattt atctagagaa ctaacagata
     7441 atagtcttcc aaaaattggg aaggaatttg ggggaaaaga tcataccaca gtcattcatg
     7501 cccatgccaa aataaaatct ttgattgatc aagacgataa tttacgttta gaaattgaat
     7561 caatcaaaaa gaaaatcaaa taatttgtgg ataactttta gttttttatc ttttttatcc
     7621 acatttttta aacaagctaa aaaacttgat atgacttgtt taaaggctgt tttccacaga
//
LOCUS       DAVLVF010000084         7301 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVLVF010000084 DAVLVF010000000
VERSION     DAVLVF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.084"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(1..1190)
                     /locus_tag="VYN66_001698"
                     /pseudo
     CDS             complement(1..1190)
                     /locus_tag="VYN66_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1399..3168
                     /locus_tag="VYN66_001699"
     CDS             1399..3168
                     /locus_tag="VYN66_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEV1495686.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            3192..5147
                     /locus_tag="VYN66_001700"
     CDS             3192..5147
                     /locus_tag="VYN66_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1495687.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEIMKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQTYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            5149..5586
                     /locus_tag="VYN66_001701"
     CDS             5149..5586
                     /locus_tag="VYN66_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1495688.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            5648..6784
                     /locus_tag="VYN66_001702"
     CDS             5648..6784
                     /locus_tag="VYN66_001702"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEV1495689.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNDFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPTQSHEDSLFVMVEPFNEWVVETKRLKNPDLRLEDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
BASE COUNT         2179 a         1261 c         1445 g         2416 t
ORIGIN      
        1 tccgatttca gaatcccaaa gaaggactcc atcatactat tgtctgggct gtttccctta
       61 cgtgacatgg atgcttgaat tcccttactc tctaggaacc gatgataaga atcgtgttgg
      121 tattgccagc cttggtcact atggagaatc gtattcttgt agtgcttctc tgtgaatgcc
      181 tgttccaaca ttgtttgtac ttgttctaag ttgggtgaag ttgaaagatt ataggcgata
      241 atttcgctat taaagccatc taaaactggt gataagtaaa gcttttgagt acttgctgga
      301 atggcaaatt ctgtcacatc tgtgtagcac ttttccattg ttttagagcc ttcaaattgg
      361 cgttgaatga gattctctgc cttcttgcca acgtctttta tgagaagaat attttcgttt
      421 ctgtcgcgtt ttagcttgta aattgagtac tttcatcaag ccttgaattc ttttatgatt
      481 taccagataa ccacgatttc ttagttctaa atgaacccgg cgataagtat aatttccctt
      541 gtgttcgata aaaatggatt gaatttcagc tttaagctct tagtccttat ctggtttatc
      601 tagctgtttc aagtgatagt agtaggtcga acgagctagt ttaatgactt ttagaagaag
      661 agctaacgaa aattcagtca ttaattcttg aacaatttct gtctttcttc tttctcctcc
      721 ttcaatcgga gttctcttaa cttttttagg atggcattct ccgctctcag gtactcattt
      781 tctgcttgaa gacgttctaa ttctgtcctc tcttcaggtc tcgtttttgg cttacgtccc
      841 attttaggta ctctccctct tgttttctca acaatagtat acccgttttt cctgtattgt
      901 gctagccagt taagaagtat cgtacgactt gggagaccgt attcaagaga aactctatct
      961 ttagtccagc cttcatgtca gactttatta ctcatttctt gttttaaatc aggagaatag
     1021 taacgatttt ttcctttttt gacgaactct attccgtaac gatcaatcaa tttaatcatg
     1081 tacctaatat tagaattgct tatcccaaat ttatttgaaa gcttctctaa gctatatcct
     1141 tgttttctaa gttcatagat ctgaacttta tcatcataag ttagtttcat aataaaaaca
     1201 ccccaaaagt tagatttttt ctgtctaact tttggggtgt agttcatgta cacctgatat
     1261 gatgcgtttt ataattttta agcctttttg cccagcctcg tcaaaagtaa tgttttgaca
     1321 caaaatctgt gacaaaactt tagttttaaa ggtttttaac tttgtatata ctagttttaa
     1381 gaaaaggagg atgatctaat ggaagaaaaa gtatcattga aagtcagggt tcaaaaacta
     1441 gggacatcgc tttcaaatat ggttatgccc aatattggag catttattgc ttggggagta
     1501 ttgactgccc tctttatcgc tgatggctat ctgccaaatg aacagttagc tactgttgtt
     1561 ggtcctatgt taacgtattt attgccaatc ctgattggtt acacaggtgg atatatgatc
     1621 catggccaac gtggtgccgt tgtaggagct attgctactg ttggtgcaat cacaggttct
     1681 agtgttccta tgtttatcgg agctatggta atgggcccac tgggaggatg gactatcaag
     1741 aaatttgatg agaagttcca agaaaaaatt cgtcccggat ttgaaatgtt agttaataac
     1801 ttctcagctg gtctcgttgg ttttgcatta ttgcttttgg ctttctacgc aatcggtcca
     1861 gtcgtatcga ctcttactgg agctgttggg aatggtgttg aggctattgt caatgctcgc
     1921 ctccttccta tggctaatat tatcatcgaa ccggctaaag tccttttcct caataatgcc
     1981 ctcaatcatg gcatttttac tcctctggga gtagaacagg tagctcaagc tggtaagtca
     2041 attctcttcc tattggaagc taatcctgga ccaggcctgg gaattctatt agcttatgct
     2101 gtattcggta aaggttctgc taaatcttct tcttgggggg caatggttat tcatttcttc
     2161 ggagggattc atgaaattta ctttccttat gttatgatga agcctactct atttttagct
     2221 gctatggcag gaggtatctc tggaactttt acttttcaac tcttagacgc tggtcttaaa
     2281 tctccagctt caccaggttc tattattgcg attatggcta cggcgccaaa aggtgtttgg
     2341 ccccatctaa atgttcttct aggtgtttta gtagcagcag ttgtttcttt ccttgtagca
     2401 gcccttattc ttcatgcaga caagtcaact gaggattcgc tcgaagctgc tcaggcggct
     2461 acccaagcag ctaaggctca gtctaaaggt cagttagtat caacttctgt tgatgcagtt
     2521 gtttcgacag actcagtgga aaaaatcatt ttcgcctgcg atgctggtat gggaagctct
     2581 gctatgggag ctagtattct tcgagataag gttaaaaaag caggtctaga gattccagta
     2641 tctaatcagg caatctcaaa tttgcttgat acaccaaaaa cattaattgt tactcaggaa
     2701 gaactgacac caagagctaa agacaagagt ccaagtgcta ttcatgtttc tgttgataat
     2761 ttcttagcgt cccctcgtta tgatgaaatt gtagcttcat taacaggagc ttctccaata
     2821 gcagaaattg aaggagatat accaacttca gcaccagtag atagtcagga aattgacctt
     2881 aaccatattg atgctgtagt agttgcttat ggtaaagcac agggaactgc aactatgggc
     2941 tgtgaaacga ttcgggctat ctttagaaac aagaatattc gtattccagt ttctactgcc
     3001 aaaatttcag aattaggtga atttaattct aaaaacataa tgattgtaac aactatttct
     3061 ttacaggcag aagtgcagca agcagcaccg aattctcaat ttcttattgt ggatagttta
     3121 gtaacaacac cagaatatga caaaatggct gctagaatgt acaaatagaa ctagaggttt
     3181 ctaaattacg aatgctatta accaaacgag aagaacaatt attgaaggct ttcctacatg
     3241 tagggaagct ttcaatgcaa gatatgactg aaatcttaca ggtttcatct agaacaattt
     3301 atcgaacttt atcagatttg acagatagca tggagcaata tggaatcgaa ataatgaagc
     3361 atgggaaata ctatattttg actggagagt tggatgattt gccgacagaa cttgaagtgt
     3421 tagttgagta tagtccccaa gaaagacaag agttgattac ctatcgcctt ctgactgaga
     3481 gcggttttgt caccaatgaa gcattgcaag agtgcacgaa agtcagtaat gtaactatta
     3541 ttcaggatat ttcagatatt gataagcgtc ttttagactt tgatctgaaa attgaacgac
     3601 aaaaaggtta tcggatttct ggtgattcag ttggtaagag aagatttttg gctattttac
     3661 tgacaaactg tatctcagta gcagattttt caaccggtaa ttttgggagc tttgatattt
     3721 tagaagcaga tagaactagg ctggccagtc agattgttaa taagcaactg tcaggttttc
     3781 cagatatgga tgctaggatg aagatgtttt ttgcgatctt gttatctctt ataggtcagg
     3841 agcaaaacat tgaaaattca cctaatacta gtaagcaggc tttggaaatt tctcaaaaaa
     3901 tttttcaaac ttactctaag cagactgcac aattttatag tattcaggaa attatctatt
     3961 ttgcgagcat cttggatgaa ttaatcatta aacgtcagga caatccgctc tttacggaga
     4021 aatttgatgg tgaatttttc tacaatattt caaatctgat tgatacggtt tccatgtata
     4081 ccaagattga cttttttaag gacaaggttt tattcaattt tcttttccat catattcggc
     4141 tcagtttagg cgtccctatc ctttttcagg gtgaaaattt gccagaatct gtccagattt
     4201 tagttgaaag gaataaattt ctttatacag tcatcagtct tttagtgaat gatatttttc
     4261 cgaaatatct tcatacagag tatgagtatg gcatgattgc cctacatttt atctctagct
     4321 taggccgtag tccagagatt tatccagtcc gtgttttgct tttaacggat gaacgtcggg
     4381 tcactagaga tttattagtc agtaaaatta agagtgttgc tccttttgta gagttgatag
     4441 atattcagtc tctagtagat taccacagta ttgatctcag tcagtatgat tatattttat
     4501 ctaccaagcc gctgactaat caggaaatcg atgtaatttc tagttttcca accgtcaaag
     4561 aattgcttga attacaggaa cgacttcagt atgtacaggc acatcgtaca attgtcgcgc
     4621 gtgatgctat cgctccagag aaaagttatg acttgcaaga ttatttaata tctagtagtc
     4681 agcttttgag tcaattcgag ttggttcaat tggagaataa tcaatcattt gagcacacgg
     4741 tagaacaaat catccaatat cagaagaatg tgagtgacag agattaccta acaagaaaat
     4801 tgttatctca cttccagaat agtcctatgg ctattcctaa tactggtctg gtgcttttac
     4861 atagtcagtc tagcaaagta acaacaaata gttttactat gtttgaactc aaactaccta
     4921 tctccgcatt gtcaatgaaa cgagaggaag aagaggtcaa aaggtgtctg ctaatgctaa
     4981 tgtctaaaga agctagcgag gaagcgagag atttaatgac agctatcagt cagtcgatta
     5041 ttgaaaatca tctttataca gagatttaca agacgggaaa tcaatccatt atttatcaga
     5101 tgctaaatac tatttttaac gaaaaaatta agaaattgga gaactaatat gaaacttgaa
     5161 aaacatttga ttaagcttaa taaacaattt tctaacaagg aggaagctat ttgttattgt
     5221 gggcaagttc tttatgaggg tggatatgtt aatgaagact atattgaagc catgattgag
     5281 cgagataaag agctatctgt ttacatgggt aactttatcg ccataccgca tggaacagat
     5341 gcagcaaaaa atgatgtcct caagtctggt attacagtcg ttcaagtccc tagaggggtt
     5401 gattttggga atgtatctaa ccctcaagtg gcaacggttc tttttggtat tgctggtatt
     5461 ggtaatgaac acttagaaat tattcagaaa atttctatct tctgtgcaga tgtagataat
     5521 gttcttaaac tagcagatgc tcagtcaaaa gaggaagtat tgcgcttatt tgatgctgtt
     5581 gaataattga atttcgtcat ttgtcatcta gtatatatgt ccctcaaata ggaaaaggag
     5641 aaattgaatg aaacattctg ttcattttgg tgccggtaat atcggtcgtg gttttatagg
     5701 tgaaattcta tttaaaaatg atttccatat tgattttgtg gatgtcaata atcagataat
     5761 tcatgctcta aatgaaaagg gcaagtatga aattgaaatt gcacagaaag gacagtctcg
     5821 tatagaagta actaatgtgg ctggaattaa tagcaaagaa catcctgagc aagtcattga
     5881 agcgattcaa aagacggata ttattactac tgcaatcgga cctaatatac tcccttttat
     5941 cgctgaactt ctagccaaag gaatcgaagc tcgccgagtt gcaggaaata cacaggcatt
     6001 ggatgttatg gcctgtgaaa atatgattgg cgggtctcaa tttctttatc aagaagtcaa
     6061 gaaatattta agtccggaag gtttgacatt tgctgataac tacataggtt ttccaaatgc
     6121 tgcagtagac aggattgttc caacacaaag tcacgaagat tccctttttg ttatggtcga
     6181 gccctttaat gaatgggtcg tggaaaccaa gcgtcttaaa aatccagatt tacgtctaga
     6241 agatgtgcat tatgaagaag atttagaacc ctttattgag cgaaaacttt tttcagtcaa
     6301 ttctggacat gcaacttcag cttacattgg tgcgcattat ggtgccaaga caattttgga
     6361 agctcttcaa aatcctaata tcaaatctcg gattgaatct gtattagctg aaattcggag
     6421 tctcttgatt gccaaatgga actttgataa aaaagaattg gagaattatc acaaagtcat
     6481 tatagaacga ttcgaaaacc ctttcatagt ggacgaggtt agtcgcgtag ctcgtactcc
     6541 aatccgaaaa ttaggctata atgaacgatt catccggccg atacgtgaat tgaaagaact
     6601 cagtttgtca tataaaaacc tacttaaaac agttggctat gcctttgact atcgcgatgt
     6661 aaatgatgaa gaaagtattc gattaggtga attgttggct aaacaatcag tcaaagatgt
     6721 tgttatacaa gttacaggtt tagacgacca agaattgatt gagcaaattg tagagtatat
     6781 ttaatctttt tcgaaaatct cttcaaatca ggttagcatc catctgcaac ctcaaaacag
     6841 tattttgagc tgacttcgtc agttctatct acaacctcaa agcagtgctt tgagctgact
     6901 ccgtcagtct tatctgcaac ctcaaaacac tgtttgagtt atctgcggta atctttctag
     6961 cttgtctttg atttttgttg ttatttataa ggtaaaagaa gctggacaaa aagtcttcaa
     7021 aatcggaaaa aggcagccta tcgggtgttc aaaaatcttg ataggatgtc ctttattatg
     7081 gaaagtctta ttggattttc tcctcagatt gagtttttga tcagctttat gagataggtt
     7141 ttgctagaga tgtagcccat catgttattt ttatggacag tgggaaaatt gttgaaaaaa
     7201 ataatgccca tcaattcttt agtcgtccaa gagaagaacg aaccaagcaa ttttggaacg
     7261 aattctttcg aatgtgatct atatagtaaa atgaaacaag a
//
LOCUS       DAVLVF010000085         7263 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVLVF010000085 DAVLVF010000000
VERSION     DAVLVF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.085"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            18..302
                     /locus_tag="VYN66_001703"
     CDS             18..302
                     /locus_tag="VYN66_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495690.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            295..533
                     /locus_tag="VYN66_001704"
                     /pseudo
     CDS             295..533
                     /locus_tag="VYN66_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            629..847
                     /locus_tag="VYN66_001705"
     CDS             629..847
                     /locus_tag="VYN66_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495691.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(1214..3538)
                     /gene="pflB"
                     /locus_tag="VYN66_001706"
     CDS             complement(1214..3538)
                     /gene="pflB"
                     /locus_tag="VYN66_001706"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV1495692.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            3932..4993
                     /gene="dinB"
                     /locus_tag="VYN66_001707"
     CDS             3932..4993
                     /gene="dinB"
                     /locus_tag="VYN66_001707"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV1495693.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            complement(5140..5985)
                     /locus_tag="VYN66_001708"
     CDS             complement(5140..5985)
                     /locus_tag="VYN66_001708"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV1495694.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(6016..6153)
                     /locus_tag="VYN66_001709"
     CDS             complement(6016..6153)
                     /locus_tag="VYN66_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495695.1"
                     /translation="MRYQTHYQLKQKLWLDPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            6240..7211
                     /locus_tag="VYN66_001710"
     CDS             6240..7211
                     /locus_tag="VYN66_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV1495696.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALKLLSDPNREQFQHDNRQVDLKILARHIHRLKKKQSDWKVQYTRCLD
                     IIFPELDKIVGKHSEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTASKIQDILSVAPR
                     SIETTSPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAVILA
                     EIRNIHAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQAAKVCARF
                     SPAFKAYLKTKLEQGKHYNVAIIHLAKKLIRTLFYILKKSCHLTNKK"
BASE COUNT         2290 a         1416 c         1423 g         2134 t
ORIGIN      
        1 tttataggag gaagacaatg gattggtacg attatatgat acaggcatcc aaacaatcac
       61 aattcaacgc aagccattgg tttcgctatt tgcgaaaagt tatttttgaa gactattctt
      121 atttaacaaa ccaagatgta gaaaagttgc tagactccaa agaactaacc cgttttcaaa
      181 aaattagctt gaagtatgcc tttcaagagc atactccaac tcataaatat gtgatttcat
      241 taaataaacc tgctaagtta accaatgttc aaaaattgat ggagaaatac aaacatggat
      301 aaaatgaaac cggtcttcca agccctaaat aagaaattaa ttcaggaaaa tctgacttta
      361 acaattatgg ttatgtctta gaatatcatg gtttacgtac cacacaagat gttgatgctt
      421 tttatgacca aaatcagaaa atcaatgaaa ttattgctcg tgtaggaaaa cagtttaatc
      481 ttaacatcca cgaagagtta tggctcaaca atcatgttgc aaagcaaata tgaacaaaca
      541 acctccctta agtttatgcg aatccttgta ctcatttgag aatctgaccg ttttagtagt
      601 ccctattgag tatgtgttag gaatgaagat gatgagtatc agagagcagg atttgcaaga
      661 tattggtgca attattaaat acaaaaactt tcattcacca ttcgatacct ttaaatattt
      721 aaaagacatg ggatttgata ctattgacct ctctgttcta ttagaaggtt ttagttatgc
      781 ttatggtatg gattggttag aaaaattttt taaagaaaat caagataaac ttcgcgaatt
      841 ttattaacag tatcaaacaa gatgattgtc aattagccag tcatcttatt tttatgatga
      901 aatgaaataa aatcggaaca aatcacttag agaattccag tcaatttcta gcactgtttg
      961 agccgtttca gcggactata gtaaaatgaa ataagaatag gacaaatcga tcaggacagt
     1021 caaatcgatt tctaagaatg ttttagaagt aaaggtgtac tattctagtt tcaatctatt
     1081 atatttcaga ttcggtccac tattccttcc caaaaacatt gcatattgcg ccgaaggaat
     1141 tgctcatact atcccaagca caaaaccctc agtcgtttga ccgagggttc cttttcatta
     1201 ttcaaaaact tgattagctc aatgcatcca aggcatcatc cattgaaaga acttcgtgga
     1261 agacacgttg tgtcaattca gttttttgtt ctggagtgag gtatttagta tttacacagt
     1321 atccagagat acgtacgata acgtcttcac ctgacataat tttttcgtaa acatcgttca
     1381 agtccataac gttcaagtta acgtgttgtc caccgttttc gaagtaacca tcaaggattg
     1441 ttaccaagtt atcaacttgt tcatcacgag tcttaccaag agcgcgaggt gatacttgtg
     1501 tagtcaatga gataccgtca gctgcataac taaagtcaag gctagaaagt gagttcaagt
     1561 tttgcaacca accaccttta gctttgttag atgggttagc acctggtgag aagaattcaa
     1621 gtttagacaa gttcacagaa ccatcttcgt tgaggtatac acctttgtga actggtgagt
     1681 taccagtttg tttagagtaa gcaacgttag atgtgattgt caaaagtgat actgtagctt
     1741 ctgcgtcttt gtatagtttg tggctacgta gacgagttgt gtaagcttcg atcaaccatt
     1801 ctgccaattc gtttgaacgt gggtcatctt caccccagcg tgggtagtca ccgattgttt
     1861 cgtaatcgta gatgtagcca tcttcgtcac ggattggttt aactgtagcg tatttgatag
     1921 ctgacaatgt atcaacagtg ttagcaaatc cacagatacc gaatcccatg ttggcacgtt
     1981 gtttagttgg caagaaggcc atttgaacag cttcgtagtt gtacctatca gtcatgtagt
     2041 ggatgatgtt caaggcatct acgtaagtgt cagtcaacca gtcaagagat ttttcaaagt
     2101 tcgctttaac tgattcgaat tcaagaactt cgtcacggat tggttcgata tcaaatactt
     2161 tgtagtcttt gtgaacatcg tcgtaaccac cattcaaacc agtaagaagg gctttaagaa
     2221 cgtttacacg agcaccgaag tactggatgt tgtggcgttg ttcttcattt tctggatcaa
     2281 gtggagacac acagcatgag atacagctca tttcaccata tccgtcttta gccattgttg
     2341 ttacaccttc gtattggata gaagagtgtt tgtggctcat gtgcatacag tagcgacgga
     2401 agttgtatgg caatttgtca gtccaaagaa ctgtcaagtt tggttctggt gagttaccga
     2461 tgttgtcaag agtgttcaag aaacggtagt ccatcttagt aacacggtga cgaccgtcgt
     2521 tacccatacc agccatagaa gttgtgataa aggttgggtc acctgagtac aattggtcat
     2581 aagcttttgt acgagcaaat ttaactgtac gaagtttcat aacgaaatca tcaacgaatt
     2641 cttggatttc tgattcagta aatgtaccac gagcaaggtc acgttctgca aagatgtcca
     2701 atacgattgg tacacgacct agagatgtag cagcaccgtt aatcacacgg cagacagcca
     2761 tgaaagcaat gttaacccat tggattgctt ctttcacgtt catcgctggt ttgcgaacat
     2821 caaccccgta aaggtcaccc aggcgaacaa cttgttgcaa tgcttggtat tgaaggttta
     2881 cttcttcacg aagacggatt gtttcttcat cgatttcttt gattgcattc cagtcattta
     2941 ctttttcttg catcaagtag tctgcaccgt aaagagcaag acgtgcgtaa acaccgatga
     3001 tacgtccgcg tgagtatgca tctggaagac cagttacagt gtgtgcgtga cgagcgcgac
     3061 gaatatttga agtgtaggca cggaaaatac cgtcgttaac tgttgttaca tatttagtga
     3121 agatttcgtg aacagctggg tctggttcgt atccattttc tttcaaagta gtttcagcca
     3181 tacggatacc accttttggc atgaagttca atttgaagag ttcatcgttt tggataccga
     3241 agataacttc attttctttg tcgataaatc cagcagggat atcagcgata gatgttggac
     3301 gagtgtccat tgggaaacga gtttcttcgt agtgtgcttt agtttcttct acaattttct
     3361 tgatgtgaag tgaacgctct gttggtcctg caaggaagct ttcgtctcca tcataaggtg
     3421 tgtagttagc ttgtacaaag cgtgatacac ttgctttttc tttccaatct acgcctttga
     3481 agccttccca agctttgtca aaaatatctt gtgcttcaac aactgtctta acaaccatat
     3541 taatgtcctc ttttttctag taacagtcat ctgttacatt catgagacaa gtataccata
     3601 tagtaaccga ttttaacaag tgaaaaatcc ctatttttac actttctttt ctaaaacagt
     3661 ctatatttta tttcaaactg tattatattt ttgaaaaaat aaagtccttt tttctttttt
     3721 tcagaaaaaa gggtataata aaagaaaata agcagtaaca ctcaatggaa atcgaaaagc
     3781 aaactaggaa gctagccgca ggctgctcaa agcactgctt tgaggttgta aatagaactg
     3841 acgaagtcag ctcaaaatac tgttttgagg ttgcagatgg aagctgacgt ggtttgaaga
     3901 gattttcgaa aagtataaaa aggtgctagg catgttgatt tttcctttgc taaatgattt
     3961 gtcaagaaaa atcatccata ttgacatgga tgcctttttt gctgcggtgg aaatcaagga
     4021 taatcctaaa ctcagaggaa aacctgtcat tattggaagc gaccctcggc aaacaggtgg
     4081 acggggagtc gtttctacct gtagctatga ggctcgagct tttggtgtcc attctgccat
     4141 gagttccaag gaagcttatg aacgttgtcc ccaggccgtc tttatctcag gaaattatga
     4201 gaaatacaaa gctgtgggac tccagattcg agctattttt aagcgctata cagatttgat
     4261 tgaacccatg agcattgacg aagcctattt ggatgtgaca gaaaataaac tcggtatcaa
     4321 gtcagcggtc aaaattgctc gcctcattca aaaagatatc tggcaagaac tccatctaac
     4381 tgcttctgcc ggtatttcct ataacaagtt cttagctaaa atggcgagtg attatcaaaa
     4441 accacatggt ttgacagtga ttctacctga acaggctgag gattttctca aacaaatgga
     4501 tatttccaaa tttcatggag taggaaaaaa gacagtagaa cgtcttcatc aaatgggcgt
     4561 ttttactggt gctgatttac ttgaagttcc tgaggtaacc ctaatagacc gttttggtag
     4621 actaggctat gatctgtatc gaaaggctcg tggcattcac aactctccag tcaaatccaa
     4681 tcgcatccgt aaatcaatcg gcaaggagaa aacctacggg aagattctcc ttgctgagga
     4741 agatatcaaa aaagagctga ctcttctatc agaaaaagtc gctctcaatc tacatcaaca
     4801 agaaaaagct ggaaaaattg tcattttgaa aatccgctac gaggactttt caactcttac
     4861 caaacgaaaa agtcttgctc aaaaaacaca agatgctagt cagataagcc aaatagccct
     4921 gcaactctat gaagaattaa gcaagaaaga aagaggtgtc cgcctattgg ggattaccat
     4981 gactggattt taaagctcaa tgaaaatcaa agagcaaact aggaggctag ccgcaggctg
     5041 ctcaaaacac tgttttgagg ttgcagataa agctgacgcg gtttgaagag attttcgaag
     5101 agtataaaac cttgaagagt tgccccttca aggtttttct tatacaaata aacggacaaa
     5161 actgtaaagt agcaaaacac taccaagcac gatacggtat ttaccaaaaa gggtgaagtc
     5221 gtgttttttc acatagctgg tcaagaagcg aatagccacc atgctgaccg caaaagctac
     5281 tcccatcgcg accaagagca aaaacaattg cccaaagctc aagagttctc cggctttcac
     5341 aaatttgaaa atctttaagg cactagctcc aaacataaca ggaatcccaa gatagaaggt
     5401 aaattctgtc acaactgaac gactggttcc atttaacaaa ccaccgacaa tcgttgcacc
     5461 tgaacggcta gtccctggta aaagagcaag aacttggaag agtccgatat agaaagcggt
     5521 cgtataagga agcttgtcca actctgttac acttggctcg atagcacgcg ctttattgcg
     5581 cttttccaaa tagatgaagg caaccccgta gataatcaac atgagagcaa ctgaaaccat
     5641 gttatggaag tgggtatcaa accaatcatc aaatttaaag acaccaagta aaggtaaagt
     5701 agcaatcaag accttcaacc atagtctcca agtcttacga acttcctgtt tgtccttagt
     5761 cggtttaaaa ggattgagct tgttaaaata aatcaccata actgctaaaa tagcaccaag
     5821 ctgaatcacg acattaaaca tggacataaa ggcttcattt tgattttggt attggatgaa
     5881 ttcctctgct aaaatcaagt gacctgtact ggaaatcggc aaccattccg taattccttc
     5941 aacaatcccg aagaagatag attttaaaat ttcaataaga tacatagatt actccctata
     6001 ttataattag cgcagttagc tactgccaaa gcccaagtgg tatacttgga ataagccact
     6061 gtggattagt tcatttcctc caatcgcttg acgatttccg aaaggatcta accacagctt
     6121 ttgtttcaat tggtaatgag tttgataacg catagacgtt ttatctcgtc taaggtaaca
     6181 aggcaaaaag ttcgtggata accaagtaga cttgaaatac tatatctgga ggtactccca
     6241 tgctttatgt gggcattgat atcgctaaaa ataaacacga tgttacagcc ttgaatgttc
     6301 caggaaaaac tgttcttaaa ccactcactt tttcaaataa taaagctggt tttgaactct
     6361 tagatctgtc tcttcgacag ctcaaccaag actgtctcat cgctcttaaa cttctttctg
     6421 atcccaatcg tgaacaattt caacacgata atcggcaagt agacctaaaa atactggcta
     6481 gacatattca tcgtctcaag aaaaaacagt ctgattggaa agtacaatac actcgttgtc
     6541 ttgatatcat ctttcctgag ttggataaaa tcgttggaaa gcattcagaa tatacctacc
     6601 aactcttgac gcgctaccct aatcctcaga aaaggattga ggcaggattt gataagctga
     6661 tagaaattaa gcgattgacc gcttctaaaa ttcaggatat cctctcagtc gcacctcgtt
     6721 ctatcgaaac aacatctcct gctcgtgaat tcgaaatcat cgaaatcatc aaacattaca
     6781 agaggctcat tgacaaggcg gaaacatgtg tcaatgactt gatggctgag ttcaactcag
     6841 tcatcacgac ggttactggg attgggggtc gtttaggggc ggtcatttta gccgagattc
     6901 gaaatattca tgcctttgat aatcctgctc aattacaagc tttcgctgga ctggattctt
     6961 ctatttatca gtcaggtcag attgatttag ctggaagaat gatcaaacgg ggttcccctc
     7021 atctgcggtg ggcactcata caagctgcca aagtatgcgc tcgcttttca cctgctttta
     7081 aggcctatct taagactaag ttagaacaag gaaaacatta caatgtagcc atcatccacc
     7141 ttgcaaaaaa acttatccga accctgtttt atatcctaaa aaagagctgc catttgacga
     7201 acaaaaagtg atgtaatttc tgaaattaac acaggattac ctttgaaaaa atggactttt
     7261 tca
//
LOCUS       DAVLVF010000086         7242 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVLVF010000086 DAVLVF010000000
VERSION     DAVLVF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.086"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(98..2356)
                     /locus_tag="VYN66_001711"
     CDS             complement(98..2356)
                     /locus_tag="VYN66_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1495697.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2585..2815)
                     /locus_tag="VYN66_001712"
     CDS             complement(2585..2815)
                     /locus_tag="VYN66_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV1495698.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3005..3688
                     /locus_tag="VYN66_001713"
     CDS             3005..3688
                     /locus_tag="VYN66_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1495699.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRLKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3688..4422
                     /locus_tag="VYN66_001714"
     CDS             3688..4422
                     /locus_tag="VYN66_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1495700.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4576..5433
                     /locus_tag="VYN66_001715"
     CDS             4576..5433
                     /locus_tag="VYN66_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV1495701.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5570..6391
                     /locus_tag="VYN66_001716"
     CDS             5570..6391
                     /locus_tag="VYN66_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEV1495702.1"
                     /translation="MILKIYNREYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGQLLSAVKAFRRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVTGYIFDGYHVAKIKDMLNLFS
                     ELYICIIPTHNKSQFENIIPTKIQDRVHYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            6405..>7242
                     /locus_tag="VYN66_001717"
     CDS             6405..>7242
                     /locus_tag="VYN66_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV1495703.1"
                     /translation="MKVIDQALLEKVIIERSRTSHKGDYGRLLFLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEAMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDAFGEDLVKQVFAGLRQNQILIVDGGALTILARTSLSFSSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASALTSFPQGTILVEKGSATRIWQAGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFAGQFKQASLYERVAVATHLHSAIAQELAQEQYVVLPTEISNCLPKVMK
                     RYV"
BASE COUNT         2067 a         1324 c         1620 g         2231 t
ORIGIN      
        1 atcataatgg acaaagggca agctgtcaag aaattacagt tattttagta aaaaaagaag
       61 cagaaaaatt tcattttcta ctccttttct ataggattta tttttctttt tcagaacttt
      121 taacttcagc ttttttggca gatttaatct gaatgtttcc cttgctagtc ataactgctt
      181 tgagatcttt ttcgcttgga ttttcaaggt agtagtcagt gattgtgtcc tcaatatagt
      241 cttgaatagt ccgacgaagt gggcgtgctc ccatttttgg atcataacct aggtcaacca
      301 acttttcctt gaccttgtca gttacatcca aacgaatgtt gttgctagag aggcgcttgt
      361 taacatctgc tagcatgagc tcgacaatct gaaggaggtt atccttgctg agagccttaa
      421 attcgataat gccatcaaaa cggttcataa actctgggct aaagaagtta ccgagttcac
      481 cgaggacaga attggtacgt ccttctctag cagcaccaaa tccaacgcta gcttcggtct
      541 ttcctgtacc tgcatttgag gtcatgataa tgatggcatc cttgaagcta acggtgcgtc
      601 cttgcccgtc tgtcaaacga ccatcgtcca agacttgaag aaacatgtgc ataacatctg
      661 ggtgagcttt ttccacttca tcgagaagga tgagagaata cggattgtgg cgaacttttt
      721 cagttaattg accagcctca tcatagccaa cataacctgg aggagcgccg accaacttag
      781 ccacactatg tttttccatg tattcactca tatcaaagcg aatcatacta tcagcagaac
      841 caaaaagttc gatagccagt tgtttggaaa gttctgtctt accgacacca gttggtccaa
      901 cgaagaggaa gcttccgatt gggcggttag gggtaccaag tccgacacga ttacggcgaa
      961 tagccttggc aatcttatcg actgcatcat cttggccaat aacatgagac ttgagatctt
     1021 cggctagatg gatgagttga gattgttctt tctctttcaa atcaccaaca gggatattgg
     1081 ttttctgctc gataatgtgc tcaatagttt tctcgctgat gataggagta tcctggtctg
     1141 tgatcttttt cttttgcatt tccttatact tggcaatctg gtcgcggaag taggccgcct
     1201 tctcaaaatc ttcttctcgt gtagcttgag acttgagatt ttcagcctca atcaagcgct
     1261 gatcaattac tttaggatcc acaaaattca aggtcaagtt catcttagaa ccagcttcat
     1321 ctaggaggtc aatggccttg tcaggcaaga agcgatcttg gatgtagcga ttggaaagag
     1381 ttgcagctgc ttcaatcgca gcatcggtat attgaacgtg gtggtaatct tcgtatttct
     1441 tttgaatccc tttgagaata gtgattgttt catccaccgt tggttcatcg actttaacag
     1501 gctgcatacg acgctcgagg gcagcatcct tttcaatgat acggtattca ttgagggtag
     1561 tagcaccgac tagttgcagt tctccacgag caagggctgg cttgaggata tttcctgcgt
     1621 ccatattacc atcactcgca gaaccagcac caacaatttc atggatttca tcgataaaga
     1681 ggatgatgtc ttcacgtttg cgaatttctt ccatgagttt ttgcatgcgt tcttcaaatt
     1741 gtcctcgaat ccccgttcct tgaactaagc taaccacatc cagacggatg acttgtttac
     1801 cttggagttt atgtggcaca tcgccatcga caattttctg agctagacct tcgacaacgg
     1861 ccgtttttcc gacaccaggt tcaccgataa ggacaggatt attcttggtt ctacgattga
     1921 gaatctcgat gacacggata atctcatcgt cgcgcccaat aacggggtca atgtctccac
     1981 gacgggcaat ttcagttaca ttaataccaa attcttccag caggcctttt tcttggctag
     2041 gtggcggagt ttgagcagat ccacgatttt gggaaccata accgccgttt ccaccgtaac
     2101 ctccacctga ttgggttggg ggaataggag gagtattgct agaaggtctg aaattgttta
     2161 gatcattgaa gaaatcacca aagggatcga agtcacgatt gttcagatcc gtcatacctt
     2221 tgaagaggct attgttagga tctgttttga taatcttata gcagttttga cagaggtcaa
     2281 tttgtttttg ttttccattg agattggtgt aaagatgaat tgttgagtcg ttaattttac
     2341 agttttgaca aagcatacat ctacctcatt tctttcttta gcctgacttg tttaaaggtc
     2401 aaaaatagtc aaattctata ctctcattat aacaggattg gggtgtaaag caagtaaaaa
     2461 gattgaagct ttgagaagtt gttacaatga aaatttttgt gaatttggac tataaaaaaa
     2521 tcctatttgt gagacaaaaa ggattttact tagacatgca gggacaatcc caacttgttt
     2581 tagattagta gagaagttcg taaatttcca ttgcgattaa gtcaatgcta tcaaacgtat
     2641 acgtttcgcg atcttctacg tcacgaaggg taaagattgg gccgttttct tcgtcgtggc
     2701 tgtacgcaac ttctgcaaca acaactcctt cgcgttcaaa attacgtttt aaatttccgc
     2761 catcgtttgc cattgcttca aggcggttga tgattctaac caaatgtgat tccattttga
     2821 ttctccttca tttcttatcg ttactatttt accataaagg agggcaactt gactagaaaa
     2881 aactaagatt tctcgctaat tctgctagtt taaagatttt taaataaatt ggataaaaaa
     2941 tttaaatttt aattcaaaac atgttataat catacagtat tctattttag aaagcagtgt
     3001 gactatgaat ttttcttttt tacctaagta tttaccttat tttaactatg gggctgttgt
     3061 gacgattctt atttctatct gtgttatctt tttgggaact attttgggtg ttgtcttggc
     3121 ttttgggcaa cgttcaaagt ttaaaccgct tgtttggttg gccaacttgt acgtttggat
     3181 tttccgtggg acaccgatga tggttcaaat tatgattgcc tttgctctta tgcatatcaa
     3241 tgctccgact attcagattg gaattttagg tgttgatttt tcgcgtctga ttccagggat
     3301 tttgattatc tctatgaata gtggtgctta tgtttcggag actgttcgtg ccggaatcaa
     3361 tgcggttcca aaaggtcagc tagaggcggc ttattcgcta gggattcgtc ttaaaaatgc
     3421 gatgcgttat gtgattttgc cacaagcagt caaaaatatc ttgccagcat tggggaacga
     3481 atttatcacc attatcaagg acagctccct cttatcagct attggggtca tggagttgtg
     3541 gaatggggct acaacagttt ctacaacaac ctatctacct ttaacaccac ttttatttgc
     3601 agcattttac tacttgatta tgacctctat tctgacagta gccttgaaag cttttgaaaa
     3661 acatatggga caaggagata agaaataatg acagaaacct tgataaaaat tgaaaattta
     3721 cataaatctt ttggaaagaa tgaagtattg aagggcatca acctcgagat taaaagagga
     3781 gaagttgtcg ttatcatcgg tccttcaggg agtgggaaat ctaccttgct tcgctctatg
     3841 aatttgttgg aggaagcaac caaggggaag cttatctttg agggagtcga tattacggac
     3901 aagaagaatg acctgtttgc catgcgtgag aagatgggca tggttttcca acaatttaac
     3961 ctctttccta atatgactgt gatggaaaat attaccttgt cacctatcaa gactaaaggt
     4021 gaaagcaggg aagttgctga gaagagagcc caagagcttt tggaaaaagt tggtttgcca
     4081 gataaggcag acgcttatcc acagagtctg tcgggtgggc agcaacaacg gattgccatc
     4141 gcgcgtgggt tggctatgga accagatgtt ttgctctttg acgaaccaac ttcagccttg
     4201 gatcctgaaa tggtaggtga ggtattggct gttatgcaag accttgccaa gtcagggatg
     4261 actatggtta tcgtaacaca tgagatggga tttgcccgtg aggtggcaga tcgtgttatc
     4321 tttatggcag acggtgtggt tgttgaagat gggacacctg agcagatttt tgaacaaacc
     4381 caagaacaac ggactaaaga tttcttgagt aaggttttat aatctatttt aaaatttcaa
     4441 gacaagatta gtgaaaagtt caaccagagc tttttcttat agtttgaaac tataggataa
     4501 cctaggaaag aagtgttaga gggcactagc agtttttggt ataatggaag atatttgaaa
     4561 gaaaagagaa gtgatatgac acagattatt gatgggaaag ctttagcggc caaattgcag
     4621 gggcagttgg ctgaaaagac tgcaaaatta aaggaagaaa cagctctagt gcctggtttg
     4681 gtagtgattt tggttgggga taatccagcc agccaagtct acgttcgcaa caaggagagg
     4741 tcagctcttg cggctggttc ccgtagcgaa gtagtgcgag ttccagagac cattactcaa
     4801 gaggaattgt tagacctgat tgctaaatac aatcaggatc cagcttggca tgggattttg
     4861 gttcagttgc cattaccaaa acacattgat gaagaggcgg ttctattggc tattgaccca
     4921 gaaaaggatg tggatggttt ccatcctcta aatatgggac gcctttggtc tggacatcca
     4981 gtcatgattc catcaacacc tgcaggaatt atggaaatgt ttcatgaata tgggattgaa
     5041 ttagaaggta aaaatgcggt tgtcatcggt cgttctaata tcgttggaaa acccatggct
     5101 cagcttcttt tggccaagaa tgcaacagtg actttgaccc actcacgcac ccataatctt
     5161 gctaaggtgg ctgctaaagc agatattctg gtggtcgcaa tcggtcgtgc caagtttgtg
     5221 actgctgact ttgtcaaacc aggtgcggta gtcattgacg ttgggatgaa tcgtgatgaa
     5281 aatggtaagc tctgtgggga tgtggattat gaggcagttg ccccacttgc tagctatatt
     5341 acgccagtcc ctggaggtgt cggccctatg accattacta tgctgatgga gcaaacttat
     5401 caagcagcac ttcggacatt ggatagaaaa taagagaaaa atcgctgaag agagtgtatt
     5461 ttctatagct atatttaaaa tggcagaaat gaatattaaa ttttagatat aagattacaa
     5521 aaggaggtct gcgcctcctt tttgttgtat aatggagtga ggtgattaga tgattttaaa
     5581 aatttataat agggaatata gtttacaatg ggatggaata tactacctag cactaattga
     5641 ttatccaaat attcaagagt gggaattaga aaaaattgct aaatttatag cttacgaaaa
     5701 acttcataaa cgtcaaacaa gtattgagtg tgctgattct tgtttaaaaa aagaaatttt
     5761 agattacatc tgtcagcatc cctttctgcc accatttacc cctacagata aaagagtagc
     5821 ctcgacttat gatctacata agaggttagt gacttcagac tactgtagtc atactacgac
     5881 tatagatgca gcgatttcta tctttaaaac aggtcagctc ttatctgctg tgaaagcctt
     5941 taggcgagat gctgaagagt tggttttgga tagtagaaat gctgcatcgg atccgataga
     6001 ttattttgat tatgtcatgt tagggtggtc aaatacaagt tctggttatc gattagcgat
     6061 ggagcgttta ttaggtcgag ctccttcaga gaaagaatta caagacaagt ttattcctgg
     6121 agtgagtttt cattttatct atacagattt gattaaagtt actggttata tttttgatgg
     6181 ctaccatgtt gcaaaaatta aggacatgtt gaatttattt agtgagttgt atatttgtat
     6241 tattccaact cataataaga gccaatttga aaatattatt ccaactaaaa tacaagatag
     6301 ggtgcattat cttgactatg ctggagaaga cttagaagag tggactaaga aagtctatca
     6361 agttgtttta aaacaatcag ataaaggata gttgaggaag aacgatgaaa gtgattgatc
     6421 aagccttact agaaaaagtt attattgaac gttctcgtac tagtcataaa ggagactatg
     6481 gccgtctgct gtttcttggt ggaacttatc cttatggtgg tgccatcatc atggctgctt
     6541 tggcagctgt aaaaagcggt gcaggattgg tgaccgttgg aacagatagg gaaaatattc
     6601 cagccctgca cagccattta cctgaggcta tggccttttc tcttcaagac cagcagttgt
     6661 taaaagagca attggagaag gcagaagttg tcttgctggg gccgggttta cgagacgatg
     6721 cttttggaga agatctagtt aaacaggtct ttgctggctt aagacaaaat cagattttga
     6781 ttgtagacgg aggtgccttg accattcttg ctaggacaag tttgtcattt tcctcaagcc
     6841 agcttatcct aactcctcac caaaaagaat gggaaaaatt atctggaatt acgattgaaa
     6901 agcaaaagga agatgcaacg gctagtgccc taacttcctt ccctcaagga acaattttgg
     6961 tggagaaagg ttcagctact cgtatttggc aagctggcca atcagattat taccagttac
     7021 aggttggcgg tccctatcag gcgactgggg gaatgggaga tacgcttgct gggatgattg
     7081 caggatttgc aggccagttt aagcaagcta gtctttacga acgtgtggca gtagcgaccc
     7141 atcttcattc agccatagcc caagaactag ctcaagaaca gtatgtggtt ttgccaacgg
     7201 agattagtaa ttgtcttcct aaagtaatga aaagatatgt ct
//
LOCUS       DAVLVF010000087         7137 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVLVF010000087 DAVLVF010000000
VERSION     DAVLVF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.087"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(344..1237)
                     /locus_tag="VYN66_001718"
     CDS             complement(344..1237)
                     /locus_tag="VYN66_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1495704.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
     gene            complement(1988..2218)
                     /gene="blpU"
                     /locus_tag="VYN66_001719"
     CDS             complement(1988..2218)
                     /gene="blpU"
                     /locus_tag="VYN66_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEV1495705.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            complement(2421..2711)
                     /locus_tag="VYN66_001720"
                     /pseudo
     CDS             complement(2421..2711)
                     /locus_tag="VYN66_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2757..2990)
                     /locus_tag="VYN66_001721"
     CDS             complement(2757..2990)
                     /locus_tag="VYN66_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1495706.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(2996..3988)
                     /gene="plsX"
                     /locus_tag="VYN66_001722"
     CDS             complement(2996..3988)
                     /gene="plsX"
                     /locus_tag="VYN66_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV1495707.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(3985..4755)
                     /gene="recO"
                     /locus_tag="VYN66_001723"
     CDS             complement(3985..4755)
                     /gene="recO"
                     /locus_tag="VYN66_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV1495708.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(4752..5921)
                     /locus_tag="VYN66_001724"
     CDS             complement(4752..5921)
                     /locus_tag="VYN66_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1495709.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            6070..7080
                     /locus_tag="VYN66_001725"
     CDS             6070..7080
                     /locus_tag="VYN66_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEV1495710.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSIVLGAICWIAIILTTLGMQTLIG
                     IF"
BASE COUNT         2027 a         1693 c         1162 g         2255 t
ORIGIN      
        1 tatttaactt ttatacgatt actacctcct ttttagttta cacttcctac atcataaata
       61 aacatactca accctcacaa tctccaaata actcttctat atcattgaaa attgagtttc
      121 atcctctata ctaatgtagg aaacctatta aaacattcct ttaatccaat ataagaatac
      181 cttgttccat taatctctac ccgatattat agatagaacc agactcctat acttaaaagg
      241 acaaataacg ttccaaccat aataaacctc ctttcttttt gagaacgttt tctttatagg
      301 tttagtatag caaaaacatg aaaaaaacaa cctgacttag aagtcaggtt gttgggaaat
      361 ctatctgttt ttctgctaaa attctctgaa ctaatacctg atcgtctagc aattctgcta
      421 gagaaatcgc cttttcatag aaactttctg cctctaaaat attcttgtct acaaataaag
      481 catattttgc ctttagcaaa aatagatgag gtcggtaaaa aacatgtctt gttttagcaa
      541 taattttctc aaatgtttga atatatatca gactatcttc tgtctgaatt tggataaata
      601 ggctcagaag aactttttcc atttgaacta ggctagcctt atcttcatat tctgaacata
      661 gcaatacttt ttgagctaac tcctcaaaat gttctttatc ttctaatccc atagcacaac
      721 aaaagaaata caattcaata atcaacaaat catttacaga aaaatatttc ttttttaata
      781 actgctggaa atattcttcc actaacccta aaccgtaggt cacatctgaa ctaccataaa
      841 tatcaaaacg agcttgtgtc acatccacaa ttaactgctc ttcctcaggc aaattctcat
      901 aatagttttc atatatttcc tcaagtatag cttcttttcg ttcgattctt cccttatcag
      961 cgtaggtttg agaatgaatc agctcatctt tgagttttag gaatcctttt ggaatctcta
     1021 taaccttaac atccacaata ttctgtaatg atactccaag tttatccgca atcaataata
     1081 atttaggaag tgttgctaaa gcttgcccat tttcaatacg tgctagctga cgaatcgtca
     1141 actctgattc atcaccacaa atatctatct gagtttttcc acaagatcgc ctaatagttt
     1201 tgattttata accaatttca cgtttcaact catccatagt aatactcctt aagttgatta
     1261 caaagatttc tttttcttcc taattctttt gtcaatcata tataaaacga taagcgaaaa
     1321 cagcggtaga acaatataac tcttttcact cattatcagc tgaaatccca gggttgctag
     1381 tatcttaaag atgaaaatat tcatcaaaaa tagaaaaaca aatactaaaa aatattttaa
     1441 aaccttcttc atttcttttc ttcaactttc ctaatttcta cacctatttt atcgaaaata
     1501 tatataaaac tccaaataaa cacttcgact gcactttttt tggcaaaaaa tagacagaac
     1561 aaacatccta tctactaaaa atatgatatg aaaggaaatt gattttttac tgtcacccta
     1621 ggcaagcttg aaacaaataa atcattgata gtaacactta cgacatccta tttattactt
     1681 caatttctaa tgtctctgct atttctaagc tacctctggt atgatctatt cctagtcaga
     1741 atagataaca ccgttctaaa ttacgattca tagttactcc ttttttgact cgttactata
     1801 agactcctct ttacatgcgc aaaataattt tgattcagat tcatcattta tagttttgat
     1861 tattttttaa cagatttttc tgttttcttc tccgaagact ttaaaaattt ttttctaatt
     1921 aaatcatcca tataaggacc aaatatacca actactaaac caataataaa acttttaaaa
     1981 tccataatta ccaccaacat gtcgctgcat aggccacacc tccaagtata gctcctcccg
     2041 cagcaccagt tgctgcacct tgccatgttc ttgttttaat tcctagctga agacctcgta
     2101 ctgctcctcc tccaacacct gctttggcaa aatctcccca attgcatccg ccaccttcaa
     2161 cgcaagcaag catctcagta tccataattt caaattgtga catctttttt gtattcataa
     2221 cgaatacctc ttttttattt ttaatatttg tcttgttaca aacttgacaa gtttagtata
     2281 acagtatcta ttaatttttt tcatccaaat cttgaattgg catcgaaacg tcttgaatta
     2341 gcttttttgt ttcaaaatca tctctatttt taaaaaagat gttttctaat cactttttta
     2401 ctattagact tcctgcaaaa ctagaatcct agttcatgat tgataatgcc agcaatcaaa
     2461 ttcattcgta atccgaagtg tttacgatga tttcgatagg ttgttgaaat cattttaaac
     2521 gtttttactt tggcaaagat gttctcaacc ttgcttctct ccttggatag cgcatggtta
     2581 taggctttat cttcagctgt tagcggcttg agtttgctgg atttacgtgg agtttgtgct
     2641 tgaagatata tcttcatgag cccttgataa ccactgtcag ccaagatttt accagcttgt
     2701 ccgatatttc tcatttctaa aaaccatcta cttccgttga cttggaactc cgattactat
     2761 tttccttgaa tgattttaac cacatctcct acgttttgga gttggtcaat ttcttcatcg
     2821 ctgatttcga tactaaattc atcttccaga gtcaagataa actccatcaa gtcaacagaa
     2881 tccgcatcca aatcgtcttt cagactcaag gattctgtca cgacaaagtc ctctccctgt
     2941 cgctcttgga taatggtcac aatacggtca aaaatttctt tttctgtcat ctcttttatt
     3001 ctcctgaaaa ttcacgcgca gtctgggcaa ccacgtctgt ttctagcatg gtacggatct
     3061 gacgaatcgt actataaaca gccttggcat cgcttgagcc atgagtcttg acaacaggtg
     3121 ctttaacacc aaacaagacc gctccaccaa catctgaata attgagctgt tttttcaaac
     3181 cactgaggct gtccttgaga aggagggcac ctagtttcgc tcgaagacca ccacctgtaa
     3241 tagctgtctt gagcaagccc atgattccca tagctgtccc ttcgatggat ttgagcacag
     3301 cgtttcccgt gaaaccatct gccacaacaa catctgcaac gccattcatc aaatcacgcg
     3361 cttccacgtt tccgataaag ttcaaacttt catcagccgc cagtaattca taagtttcct
     3421 tacgaagcgg gtcgcccttg ctactctctg ttccgttgtt gagcaaacca acgcgtggtt
     3481 gcgcaatgcc acggacattt ttagcataga aggaacctag aaccgcatat tgatggaggt
     3541 gctgggctgt attttctgca ttagcaccaa ggtctagcat gtcaaaacct tttccatcaa
     3601 cggtaggcaa ggtagacatg agtccaggac ggtcgatatt cttgatacga cccacgatga
     3661 agaatcctgc tgccaacaag gcacctgtat tcccagccga aaggacagcg tctgcttcac
     3721 catctttgac agccttggct gccaatacca tactggcatt tttcttattc ctaatagctc
     3781 tcgtaggttc atcatccgaa tcaatcttct catccgtatg gataatgctg acgcgctctg
     3841 tcgctgtcag atattgcttg attttagctt catctccgta aagttgaacc tcgatatctg
     3901 aaaagtcaga tagggcttga ttgacaccct caacaatggc ctgaggtgcg taatcgcccc
     3961 ccatggcatc tactgcgatt tttttcattt cttttcctct tttagtaatt gtccccagtc
     4021 tgctagggaa tcaataaatt tctttgattt taggtgaatc ccaacgtact cttcatatag
     4081 ttgatccata aattggcgta gctcttgctt gattccaggc ttgagcgaaa tggtctccaa
     4141 agtctcaaaa tcaatagctt gaaattgatt gagcagatag gggatattgg gattgagatg
     4201 acaacgtctc ttatcctcat gataatgctc tggacagagg caggctccat atttgaaaga
     4261 aaagtcaaaa gcctgaccaa cccgatggca gaagacacac tcattaaaat tgaggctgat
     4321 tccaaatcga gtcaaaattt gaatttcaaa aatattggtc aaaacctgat aatccaagcc
     4381 tgcttccatc aactccaaag tcttttgcaa aaaagcaaac aagggagcat cctgctgatt
     4441 gtcctgcaaa ctagcatctg caagagctgc cacataggtc gcataggcca tgacaaagag
     4501 gtcactatta atcttgggaa aagtcatgac ctcatgatag tcttcgatgt aactgagtcc
     4561 gtcatcattg attcgcaaga gaaatcgtgc cagcaccaag ggctgaataa caggcgccag
     4621 cttagactga ccagcgtgtt tgacaaaaaa catgcgtttg ccaacctgct ctgtaaaaat
     4681 tttgacgagc ttgtcatcct cacgaaaatt gcgattgtaa agcaccaagc cttgactcgt
     4741 gatagactga atcatgcttc tctcatgtac tcctcaagtc gtttcatggc ttctttgata
     4801 gtctccatgc tggctgcata agataggcgg acgtagcctt ccccgtaacg tccaaaggct
     4861 gcaccaggga taaaggcaac ggccttcttc tgagcaaaat ccttcagaaa agcaaaggag
     4921 tcttgattgt agcccgctgg aattttagca aaaatataga aggcaccgtc tggtttgata
     4981 atctcaaaac caagagcagt cattttttcg atgatatagt cccgacgttg gatatattcc
     5041 ttcttcatgg gctccgcatc gtttttacca gccgtcaagg cttctactgc agcatgttgc
     5101 gccatggtat ttgcggcagt gaccaagtac tggtgactct tgattaactg ggctgtgaag
     5161 gtcgcaggag cgaaaatcag ccccaaacgc caacctgtca tggcatgcga tttagacaaa
     5221 ccattgataa taatagcctg gtctctcaac atcgttccta gagacacatg ggcttcgcct
     5281 gtgtaggtca attctgagta aacctcatca cagacaacaa aaatttcgta cttgcgtaaa
     5341 acagctgcca aggcctctaa ctgctctcga ctgtaggtaa ttcctgtcgg attggctgga
     5401 tagttgagaa taaccgcctt gagcttatca ccctgctcca aaatggcctt ctccaacatc
     5461 tcaggagtca agacaaaacc attttcagtc gtatcaatct caacaatttc tgccccaact
     5521 aagttaacaa tcggttcata gcctggataa gcaggagctg gcaaaagtac cttgtctccc
     5581 tcttccaaaa tagccgtcaa agtcgcagat aaagcctctg tcgccccaat tgtaaccaag
     5641 atttcatttt caggagcata gtccagttgg tacttttcct taacaaagtc actggctgcc
     5701 tgacgtagag tcagcagacc actcatccct gtatagtagg attggttctg atcaatcgct
     5761 cgcttggccg cctccttgac atggtctggc gttgtaaaat caggttcccc caaggtcaaa
     5821 cgcaagaccc caggaatctc cgaaataacc tggtcaaact gacgaatcaa cgaaacttga
     5881 attttatcta actgtttatt aaagcgctta gttaagtcca tagatacctc ctactttcaa
     5941 aacgtatctc tattatacta caaaagcccc ccgaccgcaa aaatacatta gagaatagct
     6001 ttccactttc tattttactt ttcctgttta taggtctata atggtaatag attttatttg
     6061 gaggttttta tgtcatttct atcaaaaaat ggagcaggta tcttggcctg ccttctcatt
     6121 tccatcctat cttggtactt aggaggattc ttccctgtga ttggcgcgcc cgtttttgcc
     6181 attttcatag gcatgctcct acatcccttt ctctcgtcct ataaacaact ggatgctggt
     6241 ttgaccttta gttccaagaa gttgctccaa tatgccgttg tcttgcttgg ttttggtctc
     6301 aatatctcgc aggtcttcgc agttggccaa tcttcactcc ctgtcatcct gtccactatc
     6361 tcaatagctc tgattattgc ctacctcttc cagcgtttct ttgcactgga tacaaaactg
     6421 gctaccttgg ttggagtagg ttcttctatc tgtgggggtt ctgccattgc agcgacagcg
     6481 cctgttatcc atgctaagga aaaggaagta gcccaagcca tttccgttat ctttttcttc
     6541 aatgtcttgg ctgcgctcat ctttccaacc ctcggcacct ggcttcatct atccaatgaa
     6601 ggcttcgccc tctttgcagg gactgcggtc aacgacactt cctctgtaac ggctgccgcc
     6661 agcgcttggg acagtcttta ccaaagcaat accctcgagt ctgcaaccat tgttaaactc
     6721 acacgtactt tggccattat ccctatcacg ctctttctat cctactggca aagtcgccaa
     6781 caagaaaaca agcaaagcct acaactgaaa aaagtcttcc cactttttat cctttacttt
     6841 atccttgcct ctctcctcac tacactactc acctctctag gtgtgtccag tagtttcttt
     6901 acccctctca aacaactctc taaattcctt attgtcatgg ccatgagtgc tatcggtctc
     6961 aaaaccaatc tggtcgctat ggtcaaatcc agtggaaaat ccattgttct tggagccatc
     7021 tgctggattg ccatcatcct caccactctt ggtatgcaga cccttatcgg cattttctaa
     7081 cactcttcga aaatctcttc aaaccacgtc aacgtcgcct tgccgtaggt atatgtt
//
LOCUS       DAVLVF010000088         6999 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVLVF010000088 DAVLVF010000000
VERSION     DAVLVF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.088"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            27..6461
                     /locus_tag="VYN66_001726"
     CDS             27..6461
                     /locus_tag="VYN66_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1495711.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKE
                     DSNQSQGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDRNISTIRDFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIA
                     YDDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIE
                     IKATKYDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDNRIKAETKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDT
                     GEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKK
                     DAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDL
                     QREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIV
                     GIFLGLKKKNQD"
BASE COUNT         2750 a          958 c         1359 g         1932 t
ORIGIN      
        1 gttaaataaa tatgtaagga attaaaatga aaaaaagtac agtattgtca ttaaccacag
       61 ctgcagttat tttagcagcc tatgccccta atgaggtagt cttagcagac acatctagct
      121 ctgaagatgc tttaaacatc tctgataaag aaaaagtagt attagataag gaaacagaaa
      181 ataaagagaa acatgaagat attcataatg ctatagaaac ttcaaaggat actgaagaaa
      241 agaaaacaac agttattgag gaaaaagaag ttgttagtaa aaatcctgtg atagacacta
      301 aaactagcaa tgaagaagca agaatcaaag aagattccaa tcaatcccaa ggagataatg
      361 cacactcgtc tgcaaataaa ggcacagaaa atccgaaaaa agaagataga cttgtctata
      421 ttgctgaatt taaagataaa gaatctggag aaaaagcaat caagcaacta tccaatctta
      481 agaatacaaa agttttatat acttatgata gaatttttaa cggtagtgcc atagaaacaa
      541 ctccagataa cttggacaaa attaaacaaa tagaaggtat tacatcggtt gaaagggcac
      601 aaaaagtcca acccatgatg aatcatgcca gaaaggaaat tggagttgag gaagctattg
      661 attacctaaa gtctatcaat gctccgtttg ggaaaaattt tgatggtaga ggtatggtca
      721 tttcaaatat cgatactgga acagattata gacataaggc tatgagaatc gatgatgatg
      781 ccaaagcctc aatgagattt aaaaaagaag acttaaaagg aactgataaa aattattggt
      841 tgagtgataa aatccctcat gcgttcaatt attataatgg tggcaaaatc actgtagaaa
      901 aatatgatga tggaagggat tattttgacc cacatgggat gcatattgca gggattcttg
      961 ctggaaatga tactgaacaa gatatcaaaa actttaacgg catagatgga attgcaccta
     1021 atgcacaaat tttctcttac aaaatgtatt ctgacgcagg atctgggttt gcgggtgatg
     1081 aaacaatgtt tcatgctatt gaagattcta tcaaacacaa cgttgatgtt gtttcggtat
     1141 catctggttt tacaggaaca ggtcttgtag gtgagaaata ttggcaagct attcgggcat
     1201 taagaaaagc aggcattcca atggttgtcg ctacgggtaa ctatgcgact tctgcttcaa
     1261 gttcttcatg ggatttagta gcaaataatc atctgaaaat gaccgacact ggaaatgtaa
     1321 cacgaactgc agcacatgag gatgcgatag cggtcgcttc tgctaaaaat caaacagttg
     1381 agtttgataa agttaacata ggtggagaaa gttttaaata cagaaatata ggggcctttt
     1441 tcgataagaa taaaatcaca acaaatgaag atggaacaaa agctcctagt aaattaaaat
     1501 ttgtatatat aggcaagggg caagaccaag atttgatagg tttggatctt aggggcaaaa
     1561 ttgcagtaat ggatagaatt tatacaaagg atttaaaaaa tgcttttaaa aaagctatgg
     1621 ataagggtgc acgcgccatt atggttgtaa atactgtaaa ttactacaat agagataatt
     1681 ggacagagct tccagctatg ggatatgaag cggatgaagg tactaaaagt caagtgtttt
     1741 caatttcagg agatgatggt gtaaagctat ggaacatgat taatcctaat aaaaaaactg
     1801 aagtcaaaag aaataataaa gaagatttta aagataaatt ggagcaatac tatccaattg
     1861 atatggaaag ttttaattcc aacaaaccga atgtaggtga cgaaaaagag attgacttta
     1921 agtttgcacc tgacacagac aaagaactct ataaagaaga tatcatcgtt ccagcaggat
     1981 ctacatcttg ggggccaaga atagatttac ttttaaaacc cgatgtttca gcacctggta
     2041 aaaatattaa atccacgctt aatgttatta atggcaaatc aacttatggc tatatgtcag
     2101 gaactagtat ggcgactcca atcgtggcag cttctactgt tttgattaga ccgaaattaa
     2161 aggaaatgct tgaaagacct gtattgaaaa atcttaaggg agatgacaaa atagatctta
     2221 caagtcttac aaaaattgcc ctacaaaata ctgcgcgacc tatgatggat gcaacttctt
     2281 ggaaagaaaa aagtcaatac tttgcatcac ctagacaaca gggagcaggc ctaattaatg
     2341 tggccaatgc tttgagaaat gaagttgtag caactttcaa aaacacggat tctaaaggtt
     2401 tggtaaactc atatggttcc atttctctta aagaaataaa aggtgataaa aaatacttta
     2461 caatcaagct tcacaataca tcaaacagac ctttgacttt taaagtttca gcatcagcga
     2521 taactacaga ttctctaact gacagattaa aacttgatga aacatataaa gatgaaaaat
     2581 ctccagatgg taagcaaatt gttccagaaa ttcacccaga aaaagtcaaa ggagcaaata
     2641 tcacatttga gcatgatact ttcactatag gcgcaaattc tagctttgat ttgaatgcgg
     2701 ttataaatgt tggagaggcc aaaaacaaaa ataaatttgt agaatcattt attcattttg
     2761 agtcagtgga agaaatggaa gctctaaact ccaacgggaa gaaaataaac ttccaacctt
     2821 ctttgtcgat gcctctaatg ggatttgctg ggaattggaa ccacgaacca atccttgata
     2881 aatgggcttg ggaagaaggg tcaagatcaa aaacactggg aggttatgat gatgatggta
     2941 aaccgaaaat tccaggaacc ttaaataagg gaattggtgg agaacatggt atagataaat
     3001 ttaatccagc aggagttata caaaatagaa aagataaaaa tacaacatcc ctggatcaaa
     3061 atccagaatt atttgctttc aataaccaag ggatcaacgc tccatcatca agtggttcta
     3121 agattgctaa catttatcct ttagattcaa atggaaatcc tcaagatgct caacttgaaa
     3181 gaggattaac accttctcca cttgtattaa gaagtgcaga agaaggattg atttcaatag
     3241 taaatacaaa taaagaggga gaaaatcaaa gagacttaaa agtcatttcg agagaacact
     3301 ttattagagg aattttaaat tctaaaagca atgatgcaaa gggaatcaaa tcatctaaac
     3361 taaaagtttg gggtgacttg aagtgggatg gactcatcta taatcctaga ggtagagaag
     3421 aaaatgcacc agaaagtaag gataatcaag atcctgctac taagataaga ggtcaatttg
     3481 aaccgattgc agaaggtcaa tatttctata aatttaaata tagattaact aaagattacc
     3541 catggcaggt ttcctatatt cctgtaaaaa ttgataacac cgcccctaag attgtttcgg
     3601 ttgatttttc aaatcctgaa aaaattaagt tgattacaaa ggatacttat cataaggtaa
     3661 aagatcagta taagaatgaa acgctattcg cgagagatca aaaagaacat cctgaaaaat
     3721 ttgacgagat tgcgaacgaa gtttggtatg ctggcgccgc tcttgttaat gaagatggag
     3781 aggttgaaaa aaatcttgaa gtaacttacg caggtgaggg tcaaggaaga aatagaaaac
     3841 ttgataaaga cggaaatacc atttatgaaa ttaaaggtgc gggagattta aggggaaaaa
     3901 tcattgaagt cattgcatta gatggttcta gcaatttcac aaagattcat agaattaaat
     3961 ttgctaatca ggctgatgaa aaggggatga tttcctatta tctagtagat cctgatcaag
     4021 attcatctaa atatcaaaag cttggcgaga ttgcagaatc taaatttaaa aatttaggaa
     4081 atggaaaaga gggtagtcta aaaaaagata caactggggt agaacatcat catcaagaaa
     4141 atgaagagtc tattaaagaa aaatctagtt ttactattga tagaaatatt tcaacaatta
     4201 gagactttga aaataaagac ttaaagaaac tcattaaaaa gaaatttaga gaagttgatg
     4261 attttacaag tgaaactggt aagagaatag aggaatacga ttataaatac gatgataagg
     4321 gaaatattat tgcttatgac gatggtagtg ccttacaata tgaaactgaa aaacttgacg
     4381 aaataaaatc aaaaatttat ggcgttctaa gcccatctaa agatggacac tttgaaattc
     4441 ttggaaagat aagcaatgtt tctaaaaatg ccaaggtata ttatggaaat agctataaat
     4501 cgatagaaat caaagcgact aagtatgact cacattcaaa aacgatgaca tttgatttat
     4561 acgctaatat taatgatatt gtggatggat tagcttttgc tggagatatg cggttttttg
     4621 ttaaagatga taatcgaata aaagctgaaa ctaaaattag aatgcctgaa aaaaataagg
     4681 aaactaaagc agaatatccc tatgtatcaa gttatgggaa tgtaatcgaa ttaggagaag
     4741 gagatctttc aaagaacaaa ccagacaatt taactaaaat ggaatctggt aaaatctatt
     4801 ctgattcaga aaaacaacaa tatctgttaa aggataacat cattctaaga aaaggctatg
     4861 cactaaaagt gactacctat aatcctggaa aaacggatat gttagaagga aatggagtct
     4921 atagcaagga agatatagca aaaatacaaa aggccaatcc taatctaaga gtcctttcag
     4981 aaacaacaat ttatgctgat agtagaaatg ttgaagatgg aagaagtacc caagctgtat
     5041 taatgtcggc tttggacggc tttaacatta taaggtatca agtgtttaca tttaaaatga
     5101 acgataaagg ggaagctatc gataaagacg gaaatcttgt gacagattct tctaaacttg
     5161 tattatttgg taaggatgat aaagaataca ctggagagga taagtccaat gtagaagcta
     5221 taaaagaaga tggctccatg ttatttattg ataccaaacc agtaaacctt tcaatggata
     5281 agaactactt taatccatct aaatctaata aaatttatgt acgaaatcca gaattttatt
     5341 taagaggtaa gatttctgat aagggtggtt ttaactggga attgagagtt aatgaatcgg
     5401 ttgtagataa ttatttaatc tacggagatt tacacattga taacactaga gattttaata
     5461 ttaagctgaa tgttaaagac ggtgacatca tggactgggg aatgaaagac tataaagcaa
     5521 acggatttcc agataaggta acagatatgg atggaaatgt ttatcttcaa actggctata
     5581 gcgatttgaa tgctaaagca gttggagtcc actatcagtt tttatatgat aatgttaaac
     5641 ccgaagtaaa cattgatcct aagggaaata ctagtatcga atatgctgat ggaaaatctg
     5701 tagtctttaa catcaatgat aaaagaaata atggattcga tggtgagatt caagaacaac
     5761 atatttatgt aaatggaaaa gaatatacat catttgatga tattaaacaa ataacagaca
     5821 agacactaaa cattaagatt gttgtaaaag attttgcaag aaatacaacc gtaaaagaat
     5881 tcattttaaa taaagatacg ggagaggtaa gtgaattaaa acctcatagg gtaactgtga
     5941 ccattcaaaa tggaaaagaa atgagttcaa cgatagtgtc ggaagaagat tttattttac
     6001 ctgtttataa gggtgaatta gaaaaaggat accaatttga tggttgggaa atttctggtt
     6061 tcgaaggtaa aaaagacgct ggctatgtta ttaatctatc aaaagatacc tttataaaac
     6121 ctgtattcaa gaaaatagag gagaaaaagg aggaagaaaa taaacctact tttgatgtat
     6181 cgaaaaagaa agataaccca caagtaaacc atagtcaatt aaatgaaagt cacagaaaag
     6241 aggatttaca aagagaagat cattcacaaa aatctgattc aactaaggat gttacagcta
     6301 cagttcttga taaaaacaat atcagtagta aatcaactac taacaatcct aataagttgc
     6361 caaaaactgg aacagcaagc ggagcccaga cactattagc tgccggaata atgtttatag
     6421 taggaatttt tcttggattg aagaaaaaaa atcaagatta agacaaaagc tatagaaaaa
     6481 aatggtttat gtactgagat tagatagtga ggtgatgaca tagttttgtg aaaacggcca
     6541 tttataactc aattatttag tttacttcac ttcactattg atactatttg gagttattaa
     6601 cggacttagt ttagataact aatgaattga ttgaaagggt tagtattgac aatattggtc
     6661 atattgatta gaaaatagag tctatcaaaa tttaaaggct aatagaggtg atgagacaat
     6721 ttcaaggaat taagtagcgt ttggttgctt aattcctttt tatttggaag gaagtttttc
     6781 gtaaccgccc aataacgaag tctattggac ggttacgata tgatcactga cttcgtcagt
     6841 cttatctaca acctcaaaac tgtgttttga gcaacctgcg gctagcttcc tagtttactc
     6901 tttgattttc attgagtatt agaacagaaa aaatgcttgg agtatttgtt tgtgtgttta
     6961 tttttatata acaaactata aacaaaacaa aaatataaa
//
LOCUS       DAVLVF010000089         6945 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVLVF010000089 DAVLVF010000000
VERSION     DAVLVF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.089"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            70..484
                     /locus_tag="VYN66_001727"
                     /pseudo
     CDS             70..484
                     /locus_tag="VYN66_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(523..966)
                     /locus_tag="VYN66_001728"
     CDS             complement(523..966)
                     /locus_tag="VYN66_001728"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV1495712.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(996..3218)
                     /locus_tag="VYN66_001729"
     CDS             complement(996..3218)
                     /locus_tag="VYN66_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV1495713.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            3629..3871
                     /locus_tag="VYN66_001730"
     CDS             3629..3871
                     /locus_tag="VYN66_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495714.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            4211..4840
                     /locus_tag="VYN66_001731"
     CDS             4211..4840
                     /locus_tag="VYN66_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1495715.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(4963..6855)
                     /gene="pepO"
                     /locus_tag="VYN66_001732"
     CDS             complement(4963..6855)
                     /gene="pepO"
                     /locus_tag="VYN66_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV1495716.1"
                     /translation="MTRYQDDFYDAINGKWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         1879 a         1636 c         1355 g         2075 t
ORIGIN      
        1 gaatatctaa gcaatataca ttctaaaata cctgtagggg agtgtctcta aggaaaagga
       61 ggttttagca tgaaaaaaat catcttcatc aaaaccattc aactccttgt cattgatgga
      121 atcatgctgg tatttttgac atttaaaagg ggggcttact tgggactgga ttttgattta
      181 tagtggttgg ctcattttct ttcatcccgt gctattgacc tatctttcaa accaactttg
      241 tgaccacttt agtcaactct attcccagat tagaccgaga ttctggcgtt ttgctttaca
      301 aattctccta tgggatagcc tgatgattct ctccttggtg tctttaagtg atattccact
      361 tttccttcag ggaactctcc tcatcctagg acatctcatc ccttcctatc gcatctgcca
      421 aagcctgaaa agagacttcc cccaagcata tcaagaaccg atttcttttt ggagtatttt
      481 atgatcgatg agaaagacca agccgactgg gcttggtctt tcttatctct ttttagtatc
      541 taggataatg gtaacaggtc cgttattaac cagctcaacc tgcatatctg ctccaaagat
      601 acctgtctga acgggcactt cttgcgctaa tttttgattg aaagcatcat agaagtctga
      661 tgccatatca ggtttagctg cccctgtaaa ggctggacga ttgcctttct tagtatccgc
      721 aaagagggta aactgagaaa tggagaggat ttctccttca atatctttga cagacaggtt
      781 catcttgcct tctgcgtctg aaaaaatccg catattgacc agttttctca cagcatagtc
      841 caaatcttcc tcttggtcct ctggtccaac accaaccagc aataaaagtc cctgattgat
      901 ttttccctga atctggcctt ctatactcac ttgggctttt ttaacccgtt ggataatgat
      961 tttcataata gcctttctag taagagctag gacaactagc cgttggtccg tttgacagag
     1021 taaacttctg gcacactctt aattttatcg acaaccgtgg tcagtgtaga gaggttggca
     1081 ataccgaagg acacatggat attagcaaac ttcatatcct tggttggttg ggcattgacc
     1141 gttgaaatat tcttggttgt atttgaaaga acttgcagta catcgttcaa cagtcctgta
     1201 cggttgagac cgtagatatc gatatgggcc atatactcct tatttgagct agagtactgg
     1261 tcttcccatt ccacatcaag gagacgttgc tcgtagtttt cttgggcacg caggttcata
     1321 cagtccacac ggtgaatagc cacaccacga cccttggtaa tgtagccaac aatatcgtca
     1381 ccaggcacgg ggttacaaca cttagcaatc cgcactagga gaccagaagc accttcaata
     1441 accactcccc cctcatgctt gaccttgaga gtttctttat tttcaacctt gacctcgcca
     1501 cctttgacaa gctcctctgc ctcagccttg gccttggcac gctcttcctc acggcgttcc
     1561 ttttcagtca gacggttaaa gacggtaatc gcaccgattt ccccaaaacc aatggccgca
     1621 aagagggagt cttctgtctt gtaactggtc ttttgcagaa cttgatccat gtggcgcttg
     1681 tccataaatt tatttgccac atagccattt tcttggaact gagccatcag catctcacga
     1741 cccttgttga cagacaattc cttatcttgg tttttaaaga actggcgaat cttattgcgc
     1801 gccttgctag tcttgaccat attgagccag tcacggctag gtccaaagga gttcgggttg
     1861 gcgataattt caacctgatc ccctgtcttt aacttggttg tcagtggaac catgcggcca
     1921 ttgaccttgg caccagttgc tttttcaccg accttggtat ggatttcgta ggcaaaatca
     1981 atcggtcctg aatctttggg aagggaacgg acagctccat ctggggtaaa aacgtaaatc
     2041 tcctcagcca aatagttttc cttaacagag tccacaaatt ccttagcatc atcagcctgg
     2101 tcttggagct ccatcatctc cttgatccag ttcattccaa tagctgattc cttgctgtta
     2161 acttgcccct ttataccttt cttataagcc cagtgagccg caaccccgta ctcagccacc
     2221 tcgtgcattt ccttggttcg aatctggaat tcaatcggcc cttttggtcc ataaacagtc
     2281 gtatggatag actgataacc attggccttg cggttggcaa tatagtcttt gaagcgacct
     2341 ggcatcggtt tccaaaattc atgcacgtaa ccaagcatgg cataaacatc actttgggta
     2401 tctaaaatac aacgaatagc aatcagatca tagatttcct caaaccgttt tctcttgtcc
     2461 tgcattttgc ggaaaattga gtaaatatgc ttgggacgac cataaatctt ccctttcaag
     2521 tgacgttctg tcgtatactc ctctaatttt gtgactacct catccaccaa ggcctcacgc
     2581 tccctgcgct tttccttcat catatgggta atcttgtaaa actccgttgg attgagataa
     2641 cggaaagaca agtcttctaa ttcccatttg acactggaaa tccccaaacg atgggcaagt
     2701 ggggcataga tttccatggt ttctttggaa atacgctcct gcttgtcttt tcgaagatgt
     2761 ttcagggtcc gcatattgtg caagcggtca gacagtttga ccaaaataac gcggatgtcc
     2821 tcagacatgg ccatgagcat cttgcgatga ttttccgcta attgctcctc gatcgatttg
     2881 tactcgacct tgccaagctt ggtaactccg tcaacaatca cccgcacatc aggaccaaac
     2941 tctctttcca aatcgtccaa ggtcgcatct gtatcttcca ccacatcatg caagaatcca
     3001 caagctactg ttacagcatc cagctttagc ttagctaaaa tacctgccac ttggataggg
     3061 tgaatgatat aaggctcgcc tgatttgcga tattgaccac tgtggcattc aacagcatag
     3121 accaaggcct tatggacaaa atgaacatcc tcttccgtta aatattcttt ggttaaagcg
     3181 acaacttctt cgcctgttaa attcacttct ttcggcatct ctactctcca attcttccta
     3241 ccattttatc acttttttaa gaatatgaaa actagattgg aacagaataa gaaaaaaata
     3301 attcaaaatt gcttgataat tctgaattat tggcccataa tatactacga agttagattt
     3361 taaacttagg tgatagaagg agagatagaa gaacggaaac catattgtaa cccaaagact
     3421 ttctgatttc cccaattcca ttgaagatac gaaagataaa cggtggaact cgtatcacat
     3481 acactggtac cttgactgga ttttggaatt aatactcaat gaaaatcaaa gagcaaacta
     3541 ggaaactagc cacaggttgc tcaaagcact gctttgaggt tgtagataag actgacaaag
     3601 tcagtcacat atatacggca aggcgacgtt gacgcggttt gaagagattt ttgaagagta
     3661 taaaaatcct caagatactt tcttctatcc tttagtttat aaggagaata cctatgaaaa
     3721 aactgctatt tctatctttg ctctcctaat gttaggagtt tgctgcctgt tcctattcag
     3781 ccagcaaagc tataaaaaac tcgttcaata ctatgctaac gaccagaacc tgcccagtag
     3841 gataacttat agtgaatata gcgacaaatg agaagccaac tacggtagca ctctaaacat
     3901 cacgtctatc aaacaagcta atgacggagt ttatgcaacc tatgaagggc aattgacacc
     3961 tttccaatat tgataaattg ataaccagcc tgtcttcatc tagtcatgct ggtttttaag
     4021 ttcattttaa atccttacct attctcccta actgtgctat acttaattta tactcaatga
     4081 aaatcaaaga gcaaactaga aagctagccg caggctgttc aaagcactgc tttgaggttg
     4141 cagataaagt tgacgcggtt tgaagagatt ttcgaagagt attagtacat tctttgagat
     4201 tggagttagt atgaaaatcc ataaaaccgt gaatcctgtt gcctatgaaa atacctatta
     4261 tctagaaggc gaaaagcacc tcatcgtcgt cgatcctggt agtcattggg aagccattcg
     4321 tcagacaatc gagaagatca acaaaccgat ctgtgctatt ctcttgaccc acgcccatta
     4381 tgaccatatc atgagtctgg acttggttcg cgagacgttt ggcaatcctc ctgtctatat
     4441 cgcagagagc gaagccagct ggctctacac tcctgtcgat aatctctccg gtctccctcg
     4501 ccacgatgat atggcagatg tggtcacaaa acctgcagaa cacacctttg tctttcacga
     4561 agaataccaa ctagaggaat ttcgttttaa ggttctaccg accccagggc actctatcgg
     4621 tggtgtttcc ctagtctttc ctgatgctca cctagtcttg acgggagatg ctctattccg
     4681 cgaaactatc ggacggaccg accttccgac tggtagcatg gagcaactcc ttcatagtat
     4741 ccagacccaa ctcttcaccc taccaaacta cgatgtctat ccaggacatg gtccagctac
     4801 tactatcgct cacgaaaagg ccttcaatcc ctttttctag caagatgatg acaatcgaaa
     4861 tttaagtaaa ctatccagca aatctttcta ttacaaaagg catcctatca aggttttcac
     4921 acgtgattgg atgccttttt tctgatgact agattttttg tattaccaaa taatcacgcg
     4981 ctcctctggt gaacgccaca ttccgtctcc ttctttgaca tcataggttg taaagaaatc
     5041 gtcgaagttt ggtacttgca cattgacacg gagtttggct ggtgcgtgca catcgacgct
     5101 agccaaaagt ttcataaatt ctggtcgacc tttcatgcgc cagatgcgac cgaagttgta
     5161 gaagaactct tctgctgaga agtctgcttc tctcttagct gcttcaagcg ctgctgcgat
     5221 tcctcccaag tcagccacgt tttctgatac agtcaattta ccgttaatgg ttgctccata
     5281 agaatcctgt ccatcaaatt ggtcaatgac tttttgtgtt ttctccttga aggcagcata
     5341 gtcgctctct gtccaccaat ccttgaggct accattttcg tcaaaggaag ccccgttagt
     5401 atcaaaggcg tgggaaattt catgggcaat cactgcccca ataccaccgt agttagcaga
     5461 agatgactga tgcaagtcat agaaaggcgc ctgtaaaatg gccgctggaa agacaatcag
     5521 gttcttctga ggattgtagt aggcattgac catatgagca ggcatgcccc attccttata
     5581 gtctacaggc tggttccact tactccaact gtgcttgatt tccacacgcg caaaggctag
     5641 agcattctca aaaagactgg cagtttcatt cactacctta tccttgtaac gtgcaggcaa
     5701 ttcttctgga tagccaatat aaggtttgat cacattgagc ttcacgatag cctgtttaca
     5761 ggtttctgga gtgagccagt cattcttaag cagacgctcc ttatagacat caatcatggt
     5821 tgccactttt ttctccacat ccgccttggc ttctggagag aacttctcac gggcgtacca
     5881 aagccccagg gcttgcttga aaggttcctg tgctagatga taagctgctt tgaccttatc
     5941 ttttgcctct ggaactccag aaagggcacg gctgtaggca ccagacaaaa cacggatatc
     6001 ctctgttaaa tagctggttg aaagattgac aacactcaaa atcaaggttg ctttaaggag
     6061 agaccaggct tcctcactgt aaaattgctc tgctgcttgc cagaaacgtt cctcgtctac
     6121 aataaccttg tctggtaatt gcccaataac tgctttgaag aagtcatcca aaggtagggc
     6181 aggcgcgaat ttcttgaaat cttcgtaaga atatggatga tagagtttag catattctga
     6241 actttcttca ttagagagca ccactgccgc aactcggcgg tccaattcaa gtcttttttc
     6301 tagcaagtct tcaatttctt catcagagaa atcataagcc ttgaggagat ttgcgctgct
     6361 ttctttccaa agagtcaaga gctcttcgcg ctgaggatgt tcttctgcat agtaggtcgt
     6421 atctggcaag attgtgcttg gagcgctagc ccatagaaca ttgattctag catccataaa
     6481 gtctggcgat acaccaaaag gaaggaagtt tggttttcct gcaagctcaa actctgctag
     6541 tttagctgta aaatccgcaa aagtttccaa ttcttggaat tctttaagga gtggtaagac
     6601 aggtgtgata ccgtcagctt ctctcttgtc aaaatcacga actaggcggt ggtatttgac
     6661 aaagttttcc aagatagcat cctcaggcac ttcttcacct gctaaccact tgtctgttgt
     6721 cgccagcatc aggtcttcaa tttcctggtc taaatcaaca aaacctcctg tttgagactt
     6781 atctgctggg atttcagctg tctgttgcca ttttccattg atagcatcat aaaaatcatc
     6841 ttgataacgt gtcatcttgt tctcgctttc atttgtattt gcatttatct taacaaaaat
     6901 cactagggaa tgcaattaat aaccatttaa ctattatttt ttagc
//
LOCUS       DAVLVF010000090         6830 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVLVF010000090 DAVLVF010000000
VERSION     DAVLVF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.090"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            87..3437
                     /locus_tag="VYN66_001733"
     CDS             87..3437
                     /locus_tag="VYN66_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1495717.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSLLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(3943..4413)
                     /locus_tag="VYN66_001734"
     CDS             complement(3943..4413)
                     /locus_tag="VYN66_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1495718.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4430..6703)
                     /locus_tag="VYN66_001735"
     CDS             complement(4430..6703)
                     /locus_tag="VYN66_001735"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV1495719.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         2091 a         1147 c         1478 g         2114 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgagaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tagagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttgcatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgagttt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gatcaattat ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtagaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctc ttgagaaaca tatcggctat cagccaattt
      961 gctttattac aaatggcttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cacttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 atgccattgc aagcgtttgt gaagctttct ctaatcatcg tagacaggca cttttggtta
     1201 tggcaactgg ggctgggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcct
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagataaag
     1381 aaggagctca atcaagtcgc atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt tcactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caagaattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagacg
     1801 atagctgtga aaaagatatt gatgggagtg tatttaattc ctttgttttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgatca tgcggaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggacaattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgttgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctctgctt ttatttgatg aagataaaga agatgagatg gcgaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg
     2761 tttttgagca ggctgaaatt atcaggaaag tacaggagcc tgaattttgg aaagaagtta
     2821 acttgtctga tttggaaaaa attcgtcttg ctattcgaga tttattacag tttttggata
     2881 aaacagaccg taaaccctac tatgttaact ttgaagatcg tatactctcc actgttcacg
     2941 agaccacagc atttttgcag gtcaacgatc ttcggtctta caatgaaaaa gttgagcatt
     3001 atttgaaaac tcatctggat gaggagtcca tttctaagct ataccataat aaaaagttga
     3061 catctgatga tatgcttgca cttgaaaaat tgctttggga aaaattaggt agtaaagcag
     3121 actaccaaag tcattatgaa aataaggcaa ttccgagatt ggttcgtgag attattggct
     3181 tagatagaga gtctgccaat cgtatttttt ctaaattttt gtcggatgag aatcttaatg
     3241 ccaggcagat ttcatttgta aaattgattg tagactacat tgtagaaaat ggttttttag
     3301 agacgaaagt gttaacgcaa gagccgttta aatcttatgg ttctgttcaa ctactcttcc
     3361 aacaccaact accagtactt cgtaatattg ttcaaatcat tgaacttatc aataatcgag
     3421 ctggagaagc ggcttaaatt ctaaagtgat tgccatgctg agactcattt aaaattaaaa
     3481 agagtagaaa tttatgctat atatgagaag ttttattagg aagaatgtca tcgttttcct
     3541 agaatacagt atcagttgtt aagtggttga taaatttcaa agtagatact tgtaccacga
     3601 tgtttgttga tcgagttatt aacaaaagag ctactttgat tttaaagaaa tagaaaacaa
     3661 aaagccgagc aagaattcaa ttgcaggaga aaatgaaata atactcaatg aaaatcaaag
     3721 agcaaactag gaaactagct gcaggctgct caaaacactg ttttgaggtt gtagatggaa
     3781 gctgacgcgg attgaagaga ttttcgaaga gtataaatct tcctaggata aagcaaaacg
     3841 catagtatca agggttttca acacttgata ctatgcgttt tctgatgtta aagactttct
     3901 accaggtttt ttaaaagcat aattgttagt tgtagtcatt tattattctt caaagaaaaa
     3961 tggtggggcg aattttttca gttcttcaaa gcacttttga gcagtatctg catcttcaca
     4021 gatgataaga cagacatcat taccacaaag ggtagcgata gcgtcaggga agctcaaagt
     4081 atcaatgata gaaccaaagg attgagccag tccaggaagg gtttttagta ggacttggtg
     4141 ttgaactggg cgcatccaga caagggcgtc ttccatgtag agttcgagac gtttttccca
     4201 ttttgagatg gaaccattgt taagaacata ataagcgcta tcttcttcgc ggacttttga
     4261 taggttcata tttttgatgt cgcgtgagag ggttgcctgg gttacttgaa tgtcgttctc
     4321 agcaagaagg gcttgcaact cagcctgtgt atgaatcttg ttttttgtga taagagcgcg
     4381 tataagttgg tggcggtgtt ctgatttatt cataataatg taactccttt tagcaaggta
     4441 aggtaagcat ggactgagcg aggtcgacag tcaagtggta gtctgtattg tcacggatgg
     4501 tgatttcaaa gtcagtagta tagaggacta aacggagagt gtctccttct tttagcttgt
     4561 aaatagttgg ctgcagttca aattgaacgt ccatccattc atctgcagta atatcctcta
     4621 ctaacagtaa atcatttcta ttttgtaaat taaggtaacc ttttgtcacg actcgttgtg
     4681 cctctggtct aaatggcaat tcacagagat tttccaacat gtgatagcga ccgttgtcaa
     4741 tggttctagc acttaaaata gctggataag gttgtaggta tttcttttgc ccaaattcta
     4801 gcagttgggc agataagagc cccttgtttg tactggattt gatacgaaga ttgagctgag
     4861 cgcgaccgtt taggtggaga tctttagtca caggaaggtt aatagtaatc tgattggctt
     4921 tcccttgata gagctctgta ttgaaggttt ggtatgtctt accatagcgc tcaaaatcct
     4981 tatctgggta ctggttttga atagcttgct cttcttgacc aagtgagaag gtttcacagt
     5041 tttcttgccc accgaagtta tcaagtgata accaagtctg tggagctgta ttgtcctgcc
     5101 agataacagt aggaagttga aagtctgttt cctgtcctag taatttcttg gtcaataagg
     5161 catttatgga ctcacggaag tcaattgatt gccaattgtt catgtaaaca tgggcaccat
     5221 tatggaaaaa gagatgcttg tgtatatgag taggaagagc atggaacatc tggtaaacat
     5281 gaagtggttt gacattccaa tcctgagaac catgagtaaa gacaacctct gcctttactt
     5341 tatgggcatt gagcagataa ttgcggtcat gccaaaactg attgtagtcc ccagtttttc
     5401 ggtctagctg agctttcact ttttctaagt cagcttggtg agcttcattg ccacggatat
     5461 agtcgccagc taagagatta cgagaatagg ttaactcagc aagggagtca aagtcctcac
     5521 ctggataacc acctgggcta gtcaccagac cgttttcacg gtagtagttg taccatgatg
     5581 aaattcctgc ctcggcaatg ataacttcta aaccatcgac tcctgtagtc gcaagaccat
     5641 tggacatggt acctagatag gaaagtcctg ttgtagcaac ttttccgttt gaccaatcag
     5701 ccttgacttg acgctggcgc gtgtgatcag taaaggcacg gcaacggccg ttaagccaat
     5761 cgatgacatt tttataagcc tcgatttgct ggtagtctcc attagtcatg aaacctgtcg
     5821 agtctttggt accaacacct gagacataga gattggcaaa gcctctcgga aggaagtagt
     5881 cgtttagtgt atagctagag ttgatgtgag ttagcttttc ctcagcctct gctataagct
     5941 cagctttacc ttggggttgg acgagattta gttgaggttt ctctagctca atcttgtgag
     6001 gaagtttaac ctcaagctcg ccctccatct tgtagagagc cttgtcacta gccttgtcat
     6061 tggttccctg atgataaggg ctggctgtca tgatggcagg gatttttcca tcaaaacgag
     6121 ggcgaataat gctaaccttt actaggtctg atagcccttt ttggtcagta tcgacacgag
     6181 actcaacgta aacgacttca cgaatgacat cctggttaga aaaagtagcc aaactcttgc
     6241 cgttaaagta gtggtagtca ttatcctccg gaataagacc atcactaaca agttggtcga
     6301 taagagtatt tccttttttg gtgcgagtat tgagtaactg atagagattt tcaatcaagt
     6361 caccatatat aatgggaaat ccagtttctt tacgaaaaac gtcactatct tcgaagtcaa
     6421 ccaaataaga aaagcctaaa agttgaaaag caacagtata aaaaatatct gctgtcagtt
     6481 catcttctga ttgaaaaaat gtcagcaggt ctgttttttt atcagctgct aggatagaaa
     6541 gtgggtagtt ggtgtcttga taagtgaaaa agaaacgacg taaaaaggtt tcaagtgagt
     6601 ctttgtgatt ggctgtattt tgtaaatcaa agccacattt ttttagttca gataagacat
     6661 tttcttttgg aaaattgata taactatatt gattaaaacg catagaacct ccatatagaa
     6721 tgatagttaa ggttattata tcaaaaaaaa agcagaaagg gaattgttaa cttcaaaagg
     6781 aaataatcca ataaaaatga ataaagtact aaattcaata tagagaacag
//
LOCUS       DAVLVF010000091         6824 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVLVF010000091 DAVLVF010000000
VERSION     DAVLVF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.091"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..379
                     /locus_tag="VYN66_001736"
     CDS             <1..379
                     /locus_tag="VYN66_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1495720.1"
                     /translation="RGKVSGRRYQRISLVAGLTNGELIAPMTYEEMMTSDFFEAWFQK
                     FLLPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKK
                     HLKKVLPSCNTFYEALLSCSCFN"
     gene            465..1415
                     /locus_tag="VYN66_001737"
     CDS             465..1415
                     /locus_tag="VYN66_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1495721.1"
                     /translation="MGKKRWARNGSESNDASYAQVVSLYDDTSISVSNNETDKVLTGS
                     LYTETNEQGLTIPSSLLKNWNEQTGKNLTTNDLIGKSVSASIVESAAETSKIAQFQTK
                     IVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQK
                     NKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIG
                     VMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFE
                     LNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1592..2599
                     /gene="gap"
                     /locus_tag="VYN66_001738"
     CDS             1592..2599
                     /gene="gap"
                     /locus_tag="VYN66_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV1495722.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2667..3503)
                     /locus_tag="VYN66_001739"
     CDS             complement(2667..3503)
                     /locus_tag="VYN66_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1495723.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVRWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGKYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            3629..5824
                     /locus_tag="VYN66_001740"
     CDS             3629..5824
                     /locus_tag="VYN66_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1495724.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLKNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYNSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5877..6029
                     /gene="rpmG"
                     /locus_tag="VYN66_001741"
     CDS             5877..6029
                     /gene="rpmG"
                     /locus_tag="VYN66_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1495725.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6039..6215
                     /gene="secE"
                     /locus_tag="VYN66_001742"
     CDS             6039..6215
                     /gene="secE"
                     /locus_tag="VYN66_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV1495726.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6270..6806
                     /gene="nusG"
                     /locus_tag="VYN66_001743"
     CDS             6270..6806
                     /gene="nusG"
                     /locus_tag="VYN66_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV1495727.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2124 a         1190 c         1480 g         2030 t
ORIGIN      
        1 aagaggtaaa gtatctggaa gaagatatca gaggatttct ttggttgcag gtctaacaaa
       61 tggtgagtta atcgctccaa tgacttacga agagatgatg acgagcgact tttttgaagc
      121 ttggtttcag aagtttctct taccaacatt aaccacacca tcggttatta ttatggataa
      181 tgcaagattc catagaatgg gtaagttaga acttttatgc gaggagtttg ggcataaact
      241 tttacctctt cctccctact cacctgagta caatcctatt gagaaaacat gggctcatat
      301 caaaaagcac ctcaaaaagg tattaccaag ttgcaatacc ttttatgagg ctcttttgtc
      361 ctgctcttgt ttcaattgac tatagttcac ggatacagtt gggaaagaag ctattaaata
      421 tttggagagt ccttttggga tgaacatgtc aaatattact atagatgggc aagaaaagat
      481 gggcaagaaa tggatctgag tcaaacgatg cttcttatgc tcaagtagtg agtctttatg
      541 atgatacaag tatctctgtt agtaataatg agacggacaa agtgttgact ggatccctct
      601 atactgaaac aaatgaacag ggattaacga ttccaagcag tttactaaaa aattggaatg
      661 aacagacagg aaaaaatttg acaactaatg atcttattgg caaatcagtc tcagccagca
      721 ttgtagaaag tgctgccgaa actagtaaga ttgctcaatt tcaaacgaag attgtacgtg
      781 taatcaatga tgaagatgac atggaggaca gcaacagttt catgctgtct catcaaatgg
      841 aaacgatttt gaaagaggct ggatttacga aagctgtatc ttattttatc ttggaactca
      901 aagatccatc acagacaaaa gtagtaacag aagaattaca gaaaaataag aagtatactg
      961 tgctttctca acagagagtt cttgatattg tgattacctt tattcgtgtt attcagggat
     1021 tattgattgt gctttcatca caagctattg tggtagcagc ggttatgatt ggtatcatta
     1081 tttacatcaa tatcatgcaa cgttccaagg aaataggtgt catgaaagca gttggttatc
     1141 agaatcgtgg tgtcaaagga atttttattt acgaggctat ctggattgta ggcatcgcct
     1201 tgctgctggc atttttggtt gcacaagggg tgggaagttt ggcgaatgcg attgtaagtc
     1261 acttttaccc atccatcact aaggtttttg aattaaatct tttatctgtt ttaggaactc
     1321 tagttttcgc tctattactt ggttatgtct cagcctactt cccggcgcgt aagattagta
     1381 aaatggatcc tgtagaatcg ttacgctatg aataataagg gattccttga tttacagtct
     1441 taaaaagaaa ctcacataga aaaacaatgg ccatacttgc aattcaactc aaatagttgt
     1501 ataataggtt tgttgaaagg tgatttgtag tgattcaagt tactcttttc acaataaaaa
     1561 tttcatgatt ttcataagga ggaaatcact aatggtagtt aaagttggta ttaacggttt
     1621 cggacgtatc ggtcgtcttg ctttccgtcg tatccaaaac gtagaaggtg ttgaagttac
     1681 acgcatcaac gaccttacag atccagttat gcttgcacac ttgttgaaat acgacacaac
     1741 tcaaggtcgt ttcgacggta ctgttgaagt taaagaaggt ggattcgaag ttaacggtaa
     1801 attcgttaaa gtttctgctg aacgtgatcc agagcaaatc gactgggcta ctgacggtgt
     1861 agaaatcgtt cttgaagcta ctggtttctt tgctaagaaa gaagcagctg aaaaacacct
     1921 taaaggtgga gctaaaaaag ttgttatcac tgctcctggt ggaaacgacg ttaaaacagt
     1981 tgtattcaac actaaccacg acgttcttga cggtactgaa acagttatct caggtgcttc
     2041 atgtactaca aactgcttgg ctccaatggc taaagctctt caagataact ttggtgttgt
     2101 tgaaggattg atgactacta tccacgctta cactggtgac caaatgatcc ttgacggacc
     2161 acaccgtggt ggtgaccttc gccgtgctcg cgctggtgct gcaaacatcg ttcctaactc
     2221 aactggtgct gcaaaagcta tcggtcttgt aatcccagaa ttgaatggta aacttgatgg
     2281 atctgcacaa cgcgttccaa ctccaactgg atcagttact gaattggtag cagttcttga
     2341 aaagaacgtt actgttgatg aagtgaacgc agctatgaaa gcagcttcaa acgaatcata
     2401 cggttacaca gaagatccaa tcgtatcttc agatatcgta ggtatgtctt acggttcatt
     2461 gtttgacgca actcaaacta aagttcttga cgttgacggt aaacaattgg ttaaagttgt
     2521 atcatggtac gacaacgaaa tgtcatacac tgcacaactt gttcgtactc ttgaatactt
     2581 cgcaaagatt gctaaataat tcttgagttg atagaaagca aggctttgtg gtcttgcttt
     2641 tttatatgga aaaatggatg acacgatcat ccattctttt ttaattcttt ttcaaatgta
     2701 tttgaaaggg tagtgaaagt tagcctctct aaagtaagtg ggtgggtaaa ggaaagtcgg
     2761 aaggcatgaa gcataagccg gcttgtcttt gatttactat tatagagagg gtctcccagg
     2821 ataggaagat tatgatgcaa aaggtgcaca cgaatctgat gggttcgccc tgtctttagc
     2881 ttgcaatgag ccaaggaagt cttgtttgag aattgcttta atctgcttac atgcgtttca
     2941 gcatatttcc cattttttgc atcaactatt ctttttctac gatcatggcg atcacgtcca
     3001 attttgtctc tgaaaacaag ttcttttctg ttgatatttc catcaactag agcccaatat
     3061 tctctagaaa tctctttttt ctccaataag cgattgagaa tgggcaggat aaaaggattt
     3121 ttggcaaaga gaactaagcc actggtttcc atgtccagac gatgaacgac atagcaggtt
     3181 tggccaacat aggtactgac atggttaaga agggcaattt cgtttggttg attaccatgc
     3241 gttttcatcc cctctggttt gtttacaata atcaagtgtt gatcttgata aacttcctgc
     3301 actaagtctg ggttgcccca agggatcgtc ttttggggat aatcttcctc gtcaaaagtc
     3361 aactggcaaa catctccagg atttacgatt tcgttccagc ggacttcttc ttgatttatc
     3421 aaaatatgtt tcttgattct caaaaaatga cggatttttc tagggatgag gagttgttcc
     3481 tcaagtaatt gctttaccgt catttgaggt agagaggcgg gtaatgtaaa tgtgaattgc
     3541 atacagatat tgtaacaaaa aaagccctat ttggatagga aatagctaaa ttcttgtctt
     3601 cctatgatga agatgataaa ataaacgcat gaaattagat aaattatttg agaaatttct
     3661 ttctcttttt aaaaaagaaa caagtgaact agaggactct gattctacta tcttacgtcg
     3721 ctctcgtagt gatcgaaaaa aattagccca agtaggtccg attcgaaaat tctggcgtcg
     3781 ttatcatcta acaaagatta tccttatact aggtttgagt gcaggcttgc tagttggaat
     3841 ctatttgttt gctgtagcca agtcgaccaa tgtcaatgat ttgcaaaatg ccttgaaaac
     3901 tcggactctt atttttgacc gtgaagaaaa agaggctggt gccttgtctg gtcaaaaggg
     3961 aacctatgtt gagctgactg acatcagtaa aaacttgcag aatgctgtta ttgcgacaga
     4021 agaccgttct ttctataaaa atgacgggat taactatggc cgtttcttct tggctattgt
     4081 cactgctgga cgttcaggtg gtggctctac cattacccaa cagctggcta aaaacgccta
     4141 tttatcgcag gatcaaactg ttgagagaaa agcgaaagaa tttttccttg ccttagaatt
     4201 aagcaaaaaa tatagtaagg agcaaattct aaccatgtac cttaacaacg cttattttgg
     4261 aaatggtgtg tggggtgtag aagatgcgag taagaaatac tttggagttt ctgcatcaga
     4321 agtgagtctg gatcaagctg cgactctggc agggatgctc aaggggccgg aactgtataa
     4381 tcccttgaat tccgtagaag attctactaa tcggcgcgat actgtcttga agaatatggt
     4441 tgcagcagga tatattgata aaaaccaaga aaccgaagct gctgaagttg atatgacttc
     4501 gcaattgcac gataagtatg aaggaaaaat ctcagattac cgttacccct cttattttga
     4561 tgcggtggtt aatgaagctg tttccaagta taatctaaca gaggaagaga ttgtcaataa
     4621 tggctaccgc atttacacag agctggacca aaactaccaa gcaaatatgc agattgttta
     4681 tgaaaacaca tcgctatttc cgagggcaga ggatggaacg tttgctcaat caggaagtgt
     4741 agctctcgaa ccgaaaacag ggggagttcg tggagttgtc ggtcaagttg ctgacaatga
     4801 taaaactgga ttccggaatt tcaactatgc aacccaatca aagcgtagtc ctggttctac
     4861 aattaagcct ttagttgttt atacgccagc agttgaagca ggctgggctt tgaataagca
     4921 gttggataac cataccatgc agtataacag ctataaggtt gataactatg cagggatcaa
     4981 aacgagtcga gaagttccta tgtatcaagc cttggcagaa tcgcttaatc tacctgctgt
     5041 tgccactgtt aatgatttgg gtgttgacaa ggcttttgag gcaggcgaaa aattcggact
     5101 caacatggaa aaggtcgacc gtgttcttgg tgtcgccttg ggaagcggtg ttgaaaccaa
     5161 ccctcttcaa atggctcaag catacgctgc ctttgcaaat gaaggtttaa tgcctgaagc
     5221 tcattttatt agtagaattg aaaatgctag tggacaagtt attgcgagtc ataaaaattc
     5281 acaaaaacgg gtgattgata agtctgtagc tgacaagatg accagtatga tgttggggac
     5341 tttcaccaac ggtaccggta ttagttcatc gcctgcagac tatattatgg cagggaaaac
     5401 tggaacaact gaagcagttt tcaatccgga gtacacaagt gaccagtggg taattggtta
     5461 tactccggat gtagtgatta gccactggct tggctttcct accactgatg aaaatcacta
     5521 tctagctggc tctacttcaa acggtgcagc tcatgtcttt agaaacattg ccaataccat
     5581 tttaccttat acgccaggaa gtacctttac ggttgaaaat gcttataagc aaaatggaat
     5641 tgcaccagcc aatacaaaaa gacaagtaca aaccaatgat aatagccaga cagatgataa
     5701 tttgtctgat attcgagggc gtgcgcaaag tctagtagat gaggctagcc gggctatctc
     5761 agatgcgaag attaaggaaa aggctcaaac aatatgggat tcgatagtca atctatttcg
     5821 ttaagatgct tgtcaaagcc tagctttctt gttataatgg ataagatgga ggcgttatgg
     5881 cactaaaaaa agcaagccta gcttgtgcgg tttgtggttc gagaaactat tcaatcaaga
     5941 tcagcggaaa ccccaagcct acacgactag aagtaaataa attttgtaag cattgtggca
     6001 agtacactac acacagagaa acgagatagg agagagcgat gcgttttatt ggagatattt
     6061 ttagacttct taaagacaca acatggccaa ctcgcaagga aagctggaga gattttcgtt
     6121 ctatcatgga atacacagct ttctttgtag taattattta catttttgac cagttgattg
     6181 tttcaggttt gattcgattt attaacattt tttagaagat tagtggagtt aattacacta
     6241 gaaatcttct atttatgaaa ggaaatatca tggatagttt tgataaagga tggtttgttt
     6301 tacaaactta ttctggttat gaaaataagg tgaaagaaaa tctattacaa cgtgcacaaa
     6361 cctacaatat gttggataat attctacgcg ttgaaattcc aacacaaaca gtgcaagttg
     6421 aaaaaaatgg aaagagaaaa gaagtagaag aaaatcgctt tccaggttat gttcttgtag
     6481 aaatggtcat gacagatgaa gcttggtttg ttgttcgaaa cacaccaaat gttacaggat
     6541 ttgtcggatc acacgggaac agatcaaaac caactccatt attggaacaa gaaattcgtg
     6601 acattttggt atctatggga caaactgttc aagaatttga tttcgatgtt gagattggtc
     6661 aaactgtacg tattattgat ggtgcttttg cagactacac tggtaagatt acagaaattg
     6721 ataataacaa agtgaaaatg attatctcta tgtttggtaa tgacacagtt gcagaagtaa
     6781 acctaaacca aatcgcagaa ttataaccct gagagaggct ggag
//
LOCUS       DAVLVF010000092         6581 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVLVF010000092 DAVLVF010000000
VERSION     DAVLVF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.092"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(231..860)
                     /locus_tag="VYN66_001744"
     CDS             complement(231..860)
                     /locus_tag="VYN66_001744"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1495728.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(862..1644)
                     /gene="thiM"
                     /locus_tag="VYN66_001745"
     CDS             complement(862..1644)
                     /gene="thiM"
                     /locus_tag="VYN66_001745"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1495729.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPE
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(1881..2549)
                     /locus_tag="VYN66_001746"
     CDS             complement(1881..2549)
                     /locus_tag="VYN66_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV1495730.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLIMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(2764..2852)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3106..4242)
                     /gene="lctO"
                     /locus_tag="VYN66_001747"
     CDS             complement(3106..4242)
                     /gene="lctO"
                     /locus_tag="VYN66_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV1495731.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDKRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGTQTIEDVKHFKLRHNPYNPTFPVDPRDLRLY"
     gene            complement(4867..6357)
                     /gene="lysS"
                     /locus_tag="VYN66_001748"
     CDS             complement(4867..6357)
                     /gene="lysS"
                     /locus_tag="VYN66_001748"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV1495732.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYTNLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(6485..>6581)
                     /locus_tag="VYN66_001749"
     CDS             complement(6485..>6581)
                     /locus_tag="VYN66_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEV1495733.1"
                     /translation="YDLEPMAQQVIPKAAFGYIASGAGDTFTSFQ"
BASE COUNT         1952 a         1406 c         1129 g         2094 t
ORIGIN      
        1 taaagcaccc caaatgtctt tattatttaa ttatgtgatt attataacac acattttata
       61 ctagttcaag aaattgaact ggaaatacag ccttgcactc acaaagacag cagatctttc
      121 ttttgcaaaa aacaaatgac ctgtttgatg aattagccat tcaagctgaa tctggacata
      181 gctttttaaa aaaggaaaat cctacttact tagaatccaa ggatagatat ctattgttca
      241 ctcatttccc gaacagtttt ttctatattt tttgcatacg atattgccga aatgattgaa
      301 acgccatcca tattggtctt tataatgtct ttaatatgtt tcgtctgtat cccaccaatt
      361 gcaactaaag gcatttgtgg caatagtttt ctcatcaatt caagaccttc ataacctata
      421 gcaccaccag catcatcttt tgactgggta tcaaatacag gcccaacacc tacataatct
      481 acatattcaa cttttgattg ttgaaattct tcttcgtttc ttatagaaag accaattatt
      541 ttatctggca tcaattttct aatttcatca acaccaatat catcttgacc tacatgtacg
      601 ccatcggcgt caatttccat tgctaaatct atatcgtcat taacgataaa tggaacattg
      661 tattttttac aaagttcttt aatttggata gctagctcaa gtttttctaa gccttctaaa
      721 gcaccctcac ctttttctcg aaattgaaat aaggttatac caccttttaa ggcttcctca
      781 acgactgtat atagattttt tccttggcaa gtagtcgttc cacaaataaa atatagtttt
      841 agtaattctt tatgaaacat cttacttcac tcttttgaat tcctttacat cttcatctgt
      901 aatctcgtat aaggcattta taaattcaac tttaaatgtc ccaggaagat gtccatttgg
      961 acgtttttct gccatttctc cagcgatatt gtaaactaac attgctgttt ctaatgattt
     1021 taattcttga cctttttcca gtccgataaa acttgctact acagctccta ataaacatcc
     1081 agttccaata acttttggca tcatggcact accattatga atcgttacca cttctccatt
     1141 taccgcaata gcatccactt cacctgttac tactattgga atattgaact tctcatttgc
     1201 tgctagagca atctcgtcaa tattatctac tcccgcacta tctactcctt tagatgccac
     1261 atctattcct actaaagagg caatctctcc agcattcccc ctaatcgcag ctagtttgta
     1321 attgttgatt aaatcatctg ctactttttt tctatattct cctgctccac aggctacagg
     1381 atctaaaact gctgggacat tatatttctc tgcaattttc agagcagctt ggtataattt
     1441 ccaattttca tctgtcaatg ttcctatgtt tattaataaa ccaccagcat actttaataa
     1501 gtcctctaaa tcttctggaa actcactcat ggctggtgag gcgcccagtg ctactaatcc
     1561 atttgctgtg aaatttttta ctacatcatt ggtgatgcaa atgaccaaag gtgctttttc
     1621 ttttaataat tttaaacttg tcatattgaa atccttcctt ttcactttat acgatctact
     1681 aatttcgatt tatctttagt tgagaatttt tttcatttac attgaatgat ttatactcaa
     1741 tgaaaatcaa agagcaaact aggaggctaa ccgcaggttg ctcaaaacac tgttttgagg
     1801 ttgtggatag aactgacgtg gtttgaagag attttcgaag agtcttacct catcaaattt
     1861 gtaaatatca tgagccttct ctagacatcg taaccaatat caaaaaaagc taattctaaa
     1921 gcgactgctt gattccagcg ttgctgaagt tctgtcaaat cttctcgatt tttaccgaca
     1981 cgattgagtt cgtcaaccag aaattgaacc cactctgcaa agaaaggacc tctgtggaga
     2041 ttgatccatt ccgaatgaat atagacttca ggtaaagcca aatctttaga accccagtct
     2101 aaatagagac cttctgcaat gaccagcata atcaaaagat gggcatagtc tgatgaagcc
     2161 actgccgaat acattagatc ctgaaaggct tttgttacag ggtgcaaagt cacttctaga
     2221 tagtcattct ctgctacttt taactcttta aaagcctttt ggaaataacc atcttcatct
     2281 gcttcaagaa agcctagttg cttggcaaaa cgaagcttgg attcaagttt atctgcgtga
     2341 gctacgcagg cacccagcat ggataagaag gcatcaaaga agtgataatc ttgaatcaga
     2401 tagtccttta agaccttatt ctcaattgtc cccgcaaaaa gttccttaac aaaacgatga
     2461 ttgattgcag cctgccaatc cttctgactg ctttttaata attctccaac agtcaaacct
     2521 ggctgaaatg catagtcttg tgtttccata tttacttctc ctctctttac ttgttagtaa
     2581 ttaataaaac accaagaaat atcaagcaaa atcgtaattc cacttgatcc ttttaaagca
     2641 catcgagagc atttgcagag agctaactaa acaagcctat ccagtttata taaacaaaaa
     2701 actccaatta caatcaagaa ttagagttga cttacaagat tagatcgttc atttcgtcat
     2761 acgaaaaaac gtttcacatt tcccttcgcc agtcttaact gtatcaggtt caatgggtat
     2821 catctcagct taaagcaccc caaatgtctc tattatttaa ttactgaacc agtatagcaa
     2881 aaaatgaaag ccctagcaag atatttgacc gaaaaatatc tttatatata gtagattgaa
     2941 actagaatag tacacatcta cttctaaaac attgttaaaa atcgatttaa ctgtcctgat
     3001 tgatttgtcc tgtttttatt ccattttact atggttttcg atagcgattt attcttccaa
     3061 tacacgaaga aaaacctcca cattcagtgg aggcaatctc ttttatcaat acaatcttaa
     3121 gtcacgaggg tcaactggga aagttgggtt gtatgggttg tgacggagtt tgaagtgttt
     3181 aacatcttca atagtctgag ttccagacaa ctgcataact gtcttcaatt ccgcattcaa
     3241 gtgttcaaag acttgacgca caccaacact accgccgaga gccaagccat agataaccgg
     3301 gcgaccaata gctaccaagt ctgctcctga agccaaggct ttaaagacat gttgaccacg
     3361 acgaacacca gagtcaaaga caattggcac acgtttatca actgcttctg ccacttcttg
     3421 aagcgagtca aaggcagctg gtccaccgtc gatttgacga ccaccgtggt tggttaccca
     3481 gataccagaa gctcctgcag caagcgaacg ttcaacgtcc tcacggcatt gtggtccctt
     3541 gacatacaca ggaagtcctg agtattcagc gataaattct acatcacgtg gagacaagcg
     3601 ttgtttagct gatttgtaaa caaagtccat tgatttacca gcaccttctg gcaggtattc
     3661 ttcaacaatc ggcatgccaa ctgggaagac aaaaccatta cgcttatcca cttcacgatt
     3721 cccccctaca gtagcatctg ccgtcaagac aatcgcttta taaccttcag ccttcacacg
     3781 atccatgatg tggcggttga taccgtcatc cttactaaag taaaattgga accaatgggg
     3841 tgtcccttga agggcttcag aaatctctgg aaggtcaaca gtagagtaag aactggttgt
     3901 ataaagagaa ccaaactcat gcacaccacg cgcagtcgcc acttccccct gttcattcgc
     3961 taatttatgg gccgcaacag gcgccataat gattggtgaa gatagttttt cacctgcaaa
     4021 ttcaatctct gtacttggat tttcaacatc gcaaagtgta tgaggaacga tgagtttgtg
     4081 gttaaaggcg cggatattct ctcttaaagt gaaagtatct tccgccccac tagcgatata
     4141 gccaaatgct gctttaggaa taacttgttg cgccattggc tccaaatcat aggtattgat
     4201 gaagtctaca tgaccttctg cattgcttgt tttgtatgac ataaaatgcc ctccttgatt
     4261 aagtaagcgt ttgcttttta ctacacaact ataataactc ttttttcaat cattttcaag
     4321 agttttgctt aaaaaccatc ttattttttc atacaatata ctagccctac tttccttaaa
     4381 ctctaaacct tagtctatat taagtgaatc gttgagattt tttgctgcca ggcatttgaa
     4441 ctactttttt atttgcggat tctatctact aatactataa aaaaacaagt gaataagaca
     4501 ccagaacatc ctctaatagg acctatggac ttatatcagt cccatatata ctgtttaagc
     4561 aagatactat cctagttgac gctaacttcc tatcccactt attaattttt tatttatagt
     4621 gaatctgaac aaatcaattg gggaatccaa gtcaatttct agtacttttt gaacagtttc
     4681 agcgtactat cgtaaaatga aataaaacat gcacaaattg cttagggaat ttaaaccaat
     4741 ttctaacaat gtcttagaaa tcaaagtgta ctatttcaac ttcaatatac tacacttcat
     4801 tttctcctta aattcagtct ctttttgaat caaaaaatcc cctggtaaga gccagaggat
     4861 aagaatttat ttcattgttg ggaagagcaa tacatcacgg atagttgttg tatcagtgag
     4921 gagcatgcag agacggtcga taccgattcc caaaccacct gttggtggca taccgtattc
     4981 aagagcttca atgtagtcat agtcgattcc tgtcgcttca tcatcaccaa gttctttggc
     5041 tttagcttgg gcttcaaaac ggctaagttg gtcgattggg tcgttcaact cagtaaaggc
     5101 attaccgtac tccttagtca tgataaagag ctcgaaacgg tcagtaaagc gttggtcttc
     5161 aggatttttc ttagcgagtg gagatacagc tactggatgt ccatagacaa aggttggttg
     5221 gattaaagtt tcttcaacaa actcttcaaa gaaggcattg atgatgtgac caacctcagt
     5281 gtagtgtttc tcaactggaa ctttcttctc agcagcgata gctttagctt cttccaaagt
     5341 catgtcttgc cagaaatcga caccagtaat ttctctgata gcatccacca tatgaacacg
     5401 cttaaatggt tcgttaatct tgatttcagt accttggtag ttgactgggc catcaccttt
     5461 gactgattta gcagcgtgtt ggataatgcc ttcagtcaag tccatgatgt cttggaagtc
     5521 tgcataagct tggtaaactt cgatagaagt gaactcaggg ttatgagtag cgtccattcc
     5581 ttcgttacgg aagatacggc caatttcata gacacgttcc ataccaccca cgataaggcg
     5641 ttttaagtga agctcagtcg cgatacgaag caccatgtca atgttttggg cattgtggtg
     5701 ggtgataaat ggacgggcag cagcaccacc ggcttcatta tgaagaacag gtgtttccac
     5761 ttcaaggaat cctttttggt caaggtaacg acggatttca gagatgattt ttgaacgagt
     5821 gacaaagcgt tcaaagcttt cacgattaga aatcaagtca aggtaacgtt tacggtaaat
     5881 tgtttcaacg tctgtcaaac catggaattt ctcaggaaga ggacgaagag ccttagacaa
     5941 gtgtgtgatg tgggttgcct tgatagagag ttctcccata tccgtacgca tcacttcacc
     6001 ttcgacacca aggaagtcac caaggtctgc ttttttgaag atttcgtagt tttcttcacc
     6061 gacagcatcc ttacgaacgt agatctgaat ctggccttcg cggtcttgaa ggtgggcaaa
     6121 accaactttt cctttaccac gtttggttac caagcgtcct gcgatagtag ctgtttcgtt
     6181 tttatcgtgt aattgttctt tatcgaggtt ggtatattta tcttttaatt cttgtgaatt
     6241 tgcagtacgt tcaaaacgtt ttccgaaagg atcgattcct tgttcgcgga gcgcagccat
     6301 tttttcacgg cgaacgatct gctggtcatt tagttcttcc atatgttctg tagacatggg
     6361 atcctcctag ttttactcaa aattgaatac gatgagtcat tttttgcgat actcccattt
     6421 tatcataaat aagcaagaaa ttcacggata atctttaaaa actaaaaaga acctgacgct
     6481 aaaatcactg gaaagaagtg aaagtatctc ccgccccact agcgatatag ccaaatgctg
     6541 ctttaggaat aacttgttgc gccattggct ccaaatcata g
//
LOCUS       DAVLVF010000093         6551 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVLVF010000093 DAVLVF010000000
VERSION     DAVLVF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.093"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            219..482
                     /locus_tag="VYN66_001750"
     CDS             219..482
                     /locus_tag="VYN66_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV1495734.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            488..2221
                     /gene="ptsP"
                     /locus_tag="VYN66_001751"
     CDS             488..2221
                     /gene="ptsP"
                     /locus_tag="VYN66_001751"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV1495735.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            2476..4926
                     /gene="phtA"
                     /locus_tag="VYN66_001752"
     CDS             2476..4926
                     /gene="phtA"
                     /locus_tag="VYN66_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEV1495736.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            5084..>6551
                     /locus_tag="VYN66_001753"
     CDS             5084..>6551
                     /locus_tag="VYN66_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1495737.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSTNDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWLPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPS
                     NPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLG
                     AKKTDLPSSDREFYNKTYDLLARIHQDLLDNKGRQVDFE"
BASE COUNT         2226 a         1253 c         1363 g         1709 t
ORIGIN      
        1 tgtgaaatag caatgattat cctataaatt gtttttgaag aaactatatc ctgtgttttg
       61 ttatctagga tagaagattt tcagcaagtt atctgaaagg aaattgaagg aaaatccaga
      121 aaatgcttga aaaaaatcct agaagatggt ataatactaa attgtaaggg ttatcacata
      181 taactcaaaa aaagaaagaa caaaaggaga gtcaaactat ggcttctaaa gatttccacg
      241 tagtggcaga aacaggtatt cacgcacgtc cagcaacatt gttggtacaa actgctagca
      301 aatttgcttc agatatcact cttgagtaca aaggtaaatc agttaacctt aaatcaatta
      361 tgggtgttat gagtcttggt gttggtcaag gtgctgacgt aactatctca gctgaaggtg
      421 cagatgcaga tgacgctatc gctgcaatct cagaaacaat ggaaaaagaa ggattggcat
      481 aagggaaatg acagaaatgc ttaaaggaat cgcagcatct gacggtgttg cagttgcaaa
      541 agcatatcta ctcgttcagc cggatttgtc atttgagact attacagtcg aagatacaaa
      601 cgcagaagaa gctcgccttg atgccgctct acaggcatca caagacgagc tttctgttat
      661 tcgcgagaaa gcagtaggta cgctcggtga agaagcagct caagtttttg atgctcactt
      721 aatggttctt gctgacccag aaatgatcag ccaaatcaag gaaactatcc gtgcgaagaa
      781 agtgaatgca gaagcaggtc tgaaagaagt tacagatatg tttatcacta tctttgaagg
      841 catggaagac aacccataca tgcaagaacg cgcagcggat atccgcgacg tgacaaaacg
      901 tgtattggca aaccttcttg gtaaaaaatt gccaaaccca gcttctatca atgaagaagt
      961 gattgtgatt gcgcatgact tgactccttc agatacagct caattggaca aaaactttgt
     1021 aaaagctttt gtaaccaaca ttggtggacg tacaagccac tcagctatca tggcacgtac
     1081 acttgaaatt gctgctgtat taggtacaaa caacatcact gaaatcgtta aagacggtga
     1141 catccttgct gttaacggga tcactggaga agtgattatc aacccgacag atgaacaagc
     1201 ggcagaattt aaagcagctg gtgaagccta tgcgaaacaa aaagctgaat gggcactttt
     1261 gaaagatgct caaacagtga ctgctgacgg taaacacttc gagttggctg ccaatattgg
     1321 tactccaaaa gacgttgaag gtgttaacaa caacggtgca gaagctgttg gactttaccg
     1381 tacagagttc ttgtacatgg attctcaaga cttcccaact gaagatgagc agtatgaagc
     1441 atacaaggct gttcttgaag gaatgaacgg taaacctgtt gtcgttcgta caatggatat
     1501 cggtggagat aaggaacttc cttacttcga tatgcctcac gaaatgaacc cattccttgg
     1561 attccgtgct cttcgtatct ctatctctga gactggagat gctatgttcc gcacacaaat
     1621 ccgtgctctt cttcgtgcgt ctgttcacgg tcaattgcgt atcatgttcc caatggttgc
     1681 gctcttgaaa gaattccgtg cagcgaaagc agtctttgat gaagaaaaag caaaccttct
     1741 tgctgaaggt gttgcagttg cggataacat ccaagttggt atcatgatcg agattcctgc
     1801 agcggctatg cttgcagacc aatttgctaa agaagttgac ttcttctcaa ttggtacaaa
     1861 cgacttgatc caatatacaa tggcagcaga ccgtatgaac gaacaagttt cataccttta
     1921 ccaaccatac aacccatcaa tcctacgctt gattaacaat gtgatcaaag cagctcacgc
     1981 tgaaggtaaa tgggctggta tgtgtggtga gatggctggt gaccaacaag ctgttccact
     2041 tcttgtcgga atgggcttgg atgagttctc tatgtcagca acatctgtac ttcgtacacg
     2101 cagcttgatg aagaaactcg acacagctaa gatggaagag tacgcaaacc gtgcccttac
     2161 agaatgctca acaatggaag aagttcttga acttcaaaaa gaatacgtta attttgatta
     2221 atcgaaaagt ccctgcaact cagttacagg gacttttttt gatattttaa aaagaatttt
     2281 caagaaaatc tttcttataa aaagtccaac cttgaaaaag tagtggtcag aacaaaaaat
     2341 acttaaatgg ttcataaaat tcttgacaag ttggagattt aggagtaaac tattaaccag
     2401 ttaagtaata gagaggagtt tctgcaattt agaaatgaat tgcaactaga aatatcaaat
     2461 agaaagagag tttcgatgaa aattaataag aaataccttg ttggttctgc ggcagctttg
     2521 attttaagtg tttgttctta cgagttggga ctgtatcaag ctagaacggt taaggaaaat
     2581 aatcgtgttt cctatataga tggaaaacaa gcgacgcaaa aaacggagaa tttgactcct
     2641 gatgaggtta gcaagcgtga aggaatcaat gctgagcaaa tcgtcatcaa gataacagac
     2701 caaggctatg tcacttcaca tggcgaccac tatcattatt acaatggtaa ggttccttat
     2761 gatgcgattt tcagtgaaga attactcatg aaagatccaa actataagct aaaagatgag
     2821 gatattgtta atgaggtcaa gggtggatat gttatcaagg tagatggaaa atactatgtt
     2881 taccttaagg atgctgccca cgcggataac gtccgtacaa aagaggaaat caatcgacaa
     2941 aaacaagaac atagtcaaca tcgtgaaggt gggactccaa gaaacgatgg tgctgttgcc
     3001 ttggcacgtt cgcaaggacg ctatactaca gatgatggtt atatctttaa tgcttctgat
     3061 atcatagagg atactggtga tgcttatatc gttcctcatg gagatcatta ccattacatt
     3121 cctaagaatg agttatcagc tagcgagttg gctgctgcag aagccttcct atctggtcgg
     3181 ggaaatctgt caaattcaag aacctatcgc cgacaaaata gcgataacac ttcaagaaca
     3241 aactgggtac cttctgtaag caatccagga actacaaata ctaacacaag caacaacagc
     3301 aacactaaca gtcaagcaag tcaaagtaat gacattgata gtctcttgaa acagctctac
     3361 aaactgcctt tgagtcaacg acatgtagaa tctgatggcc ttgttttcga cccagcgcaa
     3421 atcacaagtc gaacagctag aggtgttgca gtgccacacg gagatcatta ccacttcatc
     3481 ccttactctc aaatgtctga attggaagaa cgaatcgctc gtattattcc ccttcgttat
     3541 cgttcaaacc attgggtacc agattcaagg ccagaacaac caagtccaca accgactccg
     3601 gaacctagtc caggcccgca acctgcacca aatcttaaaa tagactcaaa ttcttctttg
     3661 gttagtcagc tggtacgaaa agttggggaa ggatatgtat tcgaagaaaa gggcatctct
     3721 cgttatgtct ttgcgaaaga tttaccatct gaaactgtta aaaatcttga aagtaagtta
     3781 tcaaaacaag agagtgtttc acacacttta actgctaaaa aagaaaatgt tgctcctcgt
     3841 gaccaagaat tttatgataa agcatataat ctgttaactg aggctcataa agccttgttt
     3901 gaaaataagg gtcgtaattc tgatttccaa gccttagaca aattattaga acgcttgaat
     3961 gatgaatcga ctaataaaga aaaattggta gatgatttat tggcattcct agcaccaatt
     4021 acccatccag agcgacttgg caagccaaat tctcaaattg agtatactga agacgaagtt
     4081 cgtattgctc aattagctga taagtataca acgtcagatg gttacatttt tgatgaacat
     4141 gatataatca gtgatgaagg agatgcatat gtaacgcctc atatgggcca tagtcactgg
     4201 attggaaaag atagcctttc tgataaggaa aaagttgcag ctcaagccta tactaaagaa
     4261 aaaggtatcc tacctccatc tccagacgca gatgttaaag caaatccaac tggagatagt
     4321 gcagcagcta tttacaatcg tgtgaaaggg gaaaaacgaa ttccactcgt tcgacttcca
     4381 tatatggttg agcatacagt tgaggttaaa aacggtaatt tgattattcc tcataaggat
     4441 cattaccata atattaaatt tgcttggttt gatgatcaca catacaaagc tccaaatggc
     4501 tataccttgg aagatttgtt tgcgacgatt aagtactacg tagaacaccc tgacgaacgt
     4561 ccacattcta atgatggatg gggcaatgcc agtgagcatg tgttaggcaa gaaagaccac
     4621 agtgaagatc caaataagaa cttcaaagcg gatgaagagc cagtagagga aacacctgct
     4681 gagccagaag tccctcaagt agagactgaa aaagtagaag cccaactcaa agaagcagaa
     4741 gttttgcttg cgaaagtaac ggattctagt ctgaaagcca atgcaacaga aactctagct
     4801 ggtttacgaa ataatttgac tcttcaaatt atggataaca atagtatcat ggcagaagca
     4861 gaaaaattac ttgcgttgtt aaaaggaagt aatccttcat ctgtaagtaa ggaaaaaata
     4921 aactaatgaa aaatgaaagt ctcgataaag aggctttcat ttttattatg tatatatgta
     4981 aaattcttga caagcaatat taaaaagagt aaactattaa ctagttaatt aaccggttta
     5041 ttactttata gtgaatcaaa tatacttaag aaaagaggaa agaatgaaaa tcaataaaaa
     5101 atatctagct gggtcagtag ctacacttgt tttaagtgtc tgtgcttatg aactaggttt
     5161 gcatcaagct caaactgtaa aagaaaataa tcgtgtttcc tatatagatg gaaaacaagc
     5221 gacgcaaaaa acggagaatt tgactcctga tgaggttagc aagcgtgaag gaatcaatgc
     5281 cgaacaaatc gtcatcaaga taacagacca aggctatgtc acttcacatg gggaccacta
     5341 tcattattac aatggtaagg ttccttatga cgctatcatc agtgaagaat tgctgatgaa
     5401 ggatccaaac taccagctaa aggacgagga tattatcagt gaaatcaagg gtggttatgt
     5461 gattaaggta gatggaaaat actatgttta ccttaaggat gcagctcatg cggataatgt
     5521 ccgtacaaaa gaagaaatca atcggcaaaa acaagaacat agtcagcatc gtgaaggagg
     5581 gacttcaaca aacgatggtg cggtagcctt tgcacgttca caaggacgct atactacaga
     5641 tgatggttat atctttaatg cttctgatat cattgaggac acgggtgatg cttatatcgt
     5701 tcctcacggc gaccattacc attacattcc taagaatgag ttatcagcta gcgagttagc
     5761 tgctgcagaa gccttcctat ctggtcgggg aaatctgtca aattcaagaa cctatcgccg
     5821 acaaaatagc gataacactt caagaacaaa ctggctacct tctgtaagca atccaggaac
     5881 tacaaatact aacacaagca acaacagcaa cactaacagt caagcaagtc aaagtaatga
     5941 cattgatagt ctcttgaaac agctctacaa actgcctttg agtcaacgcc atgtagaatc
     6001 tgatggcctt gttttcgacc cagcgcaaat cacaagtcga accgccagag gtgtagctgt
     6061 ccctcatggt aaccattacc actttatccc ttatgaacaa atgtctgaat tggaaaaacg
     6121 aattgctcgt attattcccc ttcgttatcg ttcaaaccat tgggtaccag attcaagacc
     6181 agaacaacca agtccacaat cgactccgga acctagtcca agtctgcaac ctgcaccaaa
     6241 tcctcaacca gctccaagca atccaattga tgagaaattg gtcaaagaag ctgttcgaaa
     6301 agtaggcgat ggttatgtct ttgaggagaa tggagtttct cgttatatcc cagccaagga
     6361 tctttcagca gaaacagcag caggcattga tagcaaactg gccaagcagg aaagtttatc
     6421 gcataagcta ggagctaaga aaactgacct cccatctagt gatagagaat tttacaataa
     6481 gacttatgac ttactagcaa gaattcacca agatttactt gataataaag gtcgacaagt
     6541 tgattttgag g
//
LOCUS       DAVLVF010000094         6267 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVLVF010000094 DAVLVF010000000
VERSION     DAVLVF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.094"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            47..1747
                     /locus_tag="VYN66_001754"
     CDS             47..1747
                     /locus_tag="VYN66_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV1495738.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            1740..2216
                     /gene="ilvN"
                     /locus_tag="VYN66_001755"
     CDS             1740..2216
                     /gene="ilvN"
                     /locus_tag="VYN66_001755"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV1495739.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            2282..3304
                     /gene="ilvC"
                     /locus_tag="VYN66_001756"
     CDS             2282..3304
                     /gene="ilvC"
                     /locus_tag="VYN66_001756"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1495740.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            3411..3647
                     /locus_tag="VYN66_001757"
     CDS             3411..3647
                     /locus_tag="VYN66_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495741.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            3885..4241
                     /locus_tag="VYN66_001758"
     CDS             3885..4241
                     /locus_tag="VYN66_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1495742.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            4291..5541
                     /gene="ilvA"
                     /locus_tag="VYN66_001759"
     CDS             4291..5541
                     /gene="ilvA"
                     /locus_tag="VYN66_001759"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV1495743.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
BASE COUNT         1934 a         1043 c         1429 g         1861 t
ORIGIN      
        1 ggtatgatag taacaagtta tttttaagag gaaagaaagg ggaataatgg agaaaatcag
       61 tttagaatct cctaagacgg ggtcggacct agttttggaa acacttcgtg atttaggagt
      121 tgataccatc tttggttatc ctggtggtgc ggttttgcct ttttatgatg cgatatataa
      181 ttttaaaggc attcgccaca ttctagggcg ccatgagcaa ggttgtttgc atgaagctga
      241 aggctatgcc aaatcaactg gaaagttggg tgttgccgtc gtcactagtg gaccaggagc
      301 aacaaatgcc attacaggga ttgcggatgc tatgagcgat agcgttcccc ttttggtctt
      361 tacaggtcag gtggcgcgag cagggattgg gaaggatgcc tttcaggagg cagacatcgt
      421 aggaattacc atgccaatca ctaagtacaa ttaccaagtt cgtgagacag ctgatattcc
      481 gcgtatcatt acggaagctg tccatatcgc aactacaggc cgtccagggc cagttgtaat
      541 tgacctacca aaagacatat ctgctttaga aacagacttc atttattcac cagaagtgaa
      601 tttaccaagt tatcagccga ctcttgagcc gaatgatatg caaatcaaga aaatcttgaa
      661 gcaattgtcc aaggctaaaa agccagtctt gttagctggt ggtggaatta gttatgctga
      721 ggctgctacg gaactaaatg aatttgcaga acgctatcaa attccagtgg taaccagtct
      781 tttgggacaa ggaacgattg caacgagtca cccactcttt cttggaatgg gaggcatgca
      841 cgggtcattc gcagcaaata ttgccatgac ggaagcggac tttatgatta gtattggttc
      901 tcgtttcgat gaccgtttga cggggaatcc taagactttc gctaagaatg ctaaggttgc
      961 ccacattgat attgacccag ctgagattgg caagattatc agtgcagaca ttcctgtagt
     1021 tggagatgct aagaaggcct tgcaaatgtt gctagcagaa ccaacagttc acaacaatac
     1081 tgaaaagtgg attgagaaag tcactaaaga caagaatcgt gttcgttcgt atgacaagaa
     1141 agagcgtgtg gttcaaccgc aagcagttat tgaacgaatt ggtgaattga cgaatggaga
     1201 tgccattgtg gtaacagacg ttggtcaaca ccaaatgtgg acagctcagt attatcccta
     1261 ccaaaatgaa cgtcagttag tgacttcagg tggtttggga acaatgggct ttggaattcc
     1321 agcagcaatc ggtgctaaaa ttgctaaccc agataaggaa gtagtcttgt ttgttgggga
     1381 tggtggtttc caaatgacca accaggagtt ggctattttg aatatttaca aggtgccaat
     1441 caaggtggtt atgctgaaca atcattcact cggaatggtt cgccagtggc aggaatcctt
     1501 ctatgaaggc agaacatcag agtcggtctt tgataccctt cctgatttcc aattgatggc
     1561 gcaggcttat ggtattaaaa actataagtt tgacaatcct gagaccttgg ctcaagacct
     1621 tgaagtcatc actgaggatg ttcctatgct aattgaggta gatatttctc gtaaggaaca
     1681 ggtgttacca atggtaccgg ctggtaagag taatcatgag atgttggggg tgcagttcca
     1741 tgcgtagaat gttaacagca aaactacaaa atcgttcagg agtcctcaat cgctttacag
     1801 gtgtcctatc tcgtcgtcag gttaatattg aaagcatctc tgttggagca acagaagatc
     1861 cgaatgtatc gcgtatcact attattattg atgttgcttc tcatgatgaa gtggagcaaa
     1921 tcatcaaaca gctcaatcgt cagattgatg tgattcgcat tcgagatatt acagacaagc
     1981 ctcatttgga gcgcgaggtg attttggtta agatgtcagc gccagctgag aagagagctg
     2041 agattttagc gattattcaa cctttccgtg caacagtggt agacgtagcg ccaagctcga
     2101 ttaccattca gatgacggga aatgcagaaa agagcgaagc cctattgcga gtcattcgcc
     2161 catacggtat tcgcaatatt gctcgaacgg gtgcaactgg atttacccgc gattaaaaat
     2221 ccaacttaaa tttgttaaac cagcttaaaa ggcaataaat aatagaaaag agagaaaagc
     2281 tatgacagtt caaatggaat atgaaaaaga tgttaaagta gcagcacttg acggtaaaaa
     2341 aatcgccgtt atcggttatg gttcacaagg gcatgcgcat gctcaaaact tgcgtgattc
     2401 aggtcgtgac gttattatcg gtgtacgtcc aggtaaatct tttgataaag caaaagaaga
     2461 tggatttgat acttacacag tagcagaagc tactaagttg gctgatgtta tcatgatctt
     2521 ggcgccagac gaaattcaac aagaattgta cgaagcagaa atcgctccaa acttggaagc
     2581 tggaaacgca gttggatttg cccatggttt caacatccac tttgaattta tcaaagttcc
     2641 tgcggatgta gatgtcttca tgtgtgctcc taaaggacca ggacacttgg tacgtcgtac
     2701 ttacgaagaa ggatttggtg ttccagctct ttatgcagta taccaagatg caacaggaaa
     2761 tgctaaaaac attgctatgg actggtgtaa aggtgttgga gcggctcgtg taggtcttct
     2821 tgaaacaact tacaaagaag aaactgaaga agatttgttt ggtgaacaag ctgtactttg
     2881 tggtggtttg actgccctta tcgaagcagg tttcgaagtc ttgacagaag caggttacgc
     2941 tccagaattg gcttactttg aagttcttca cgaaatgaaa ttgatcgttg acttgatcta
     3001 cgaaggtgga ttcaagaaaa tgcgtcaatc tatttcaaac actgctgaat acggtgacta
     3061 tgtatcaggt ccacgtgtaa tcactgaaca agttaaagaa aatatgaagg ctgtcttggc
     3121 agacatccaa aatggtaaat ttgcaaatga ctttgtaaat gactataaag ctggacgtcc
     3181 aaaattgact gcttaccgtg aacaagcagc taaccttgaa attgaaaaag ttggtgcaga
     3241 attgcgtaaa gcaatgccat tcgttggtaa aaacgacgat gatgcattca aaatctataa
     3301 ctaattagaa atatatagcg ctggagatga ttttatgaaa aagattatga gaaaaattgc
     3361 atcgttatta ttggttctag ttgtataatg taattacacc gtcggtaata gtgctagcag
     3421 accaaaataa agcagattgg tcgtatgatg aaaatgctgt aattaacatt tatgatgatg
     3481 ctaattttga agatggtagg ttgcatatga cctttgaaca attcttcaaa ttggcacaaa
     3541 tagctagaga agaaggtctt gaaattcatt ctccgtttga gagagctggt gcgactaaat
     3601 ctgctcgtta tatagcgaaa tggattttga gaaataaaaa atattaacaa atatagttgg
     3661 taaatcatta ggacctaaat cagctgttag attcggagaa gctttatcct atattgaagg
     3721 tcctcttcgc agaataaatg agacgataga tggcggttta tatcaaatag agcaaattat
     3781 tgcatctgga ttgaaagaat cgggtttaaa tgactggact gcgaaaactt tagcttcagc
     3841 tattcgtggg atattagatg tacttattta ggggttgaaa tcatatgaat attaccaatt
     3901 tgttttctat caagacagga tgtgatgaaa ctgataggca actgcaaaaa ctattttttc
     3961 agttggattt acaattggga gaattgacag atcaactaag aaaattagat tctaattttg
     4021 ttcctcgtag tcaatttgta gacacgttgg atttgaatga tgtagaatat aaagaaattt
     4081 taaactattt tatcttccat cgtaatgata gtgaagaaag tttggtagaa tggttatatg
     4141 attggatttc cacaaatcgt tatgaacttc ctaaagagtt ttcgattcgt atggctcata
     4201 aataccatga aagtgttact gaaattttcg gagatgaata actaaaaaac agtcattagt
     4261 gactgttttt tatagaaaaa gaggttttat atgttaagtt caaaagatat aatcaaggct
     4321 cacaaggtct tgaacggtgt ggttgtgaat actccactgg attacgatca ttatttatcg
     4381 gagaagtatg gtgctaagat ttatttgaaa aaagaaaatg cccagcgtgt tcgctccttt
     4441 aaaattcgtg gtgcctatta tgccatttcc cagctcagca aggaagaacg tgaacgtggg
     4501 gtagtttgcg cttctgcggg aaatcatgcg cagggagtag cctatacttg taatgaaatg
     4561 aaaattcctg ctactatctt tatgcccatt actacgccac aacaaaagat tggtcaggtt
     4621 cgcttttttg gtggggattt tgtaactatt aaactagttg gagatacctt tgatgcctca
     4681 gccaaagcag ctcaagaatt tacagtctct gaaaatcgta cctttattga tccttttgat
     4741 gatgctcatg ttcaagcagg tcaaggaaca gttgcttatg agattttaga agaagctcga
     4801 aaagaatcga ttgattttga tgctgtcttg gttcctgttg gtggtggcgg tctcattgcc
     4861 ggggtttcta cctatatcaa ggaaacaagt ccagagattg aggttatcgg agtagaggcg
     4921 aatggagcgc gttccatgaa agctgccttt gaggctggag gtccagtcaa actcaaggaa
     4981 attgataaat ttgcggatgg gattgctgtg caaaaggtag gtcagttgac ctatgaagca
     5041 actcgtcaac atattcaaac tttggtaggt gtcgatgagg gattgatttc tgaaaccttg
     5101 attgaccttt actctaagca agggatagtc gcagaacctg ctggagcagc tagtatcgcc
     5161 tctttagagg ttttagctga atatattaag gggaaaacca tttgttgtat catttctgga
     5221 ggaaataatg atatcaaccg tatgccagaa atggaagagc gtgccttgat ttatgatggt
     5281 atcaaacatt attttgtggt caatttccca caacgtccag gagctttgcg tgagtttgta
     5341 aatgatatcc tggggccaaa tgatgatatc acacgttttg agtatatcaa acgagctagc
     5401 aagggaacag gcccagtatt gattgggatc gctttagcag ataagcatga ttatgcaggt
     5461 ttgattcgta gaatggaagg ttttgatcca gcttatatta acttaaatgg taatgaaacg
     5521 ctttataata tgcttgtctg aggactaata aaaaaatatc atattatttg tacaactgat
     5581 gtatatatgc tataatgaag atgaagaaag gggacgattc ctatggtttt agaaaactta
     5641 tcgtgcaatt cttacttctg ttcattatag cacttatgat gtttggctca gatttccatc
     5701 cttcattttg atttcctatc tattgacaag catagtcaca ctgtctttaa tactcttcga
     5761 aaatctcttc aaaccacgtt agctctatct gcaacctcaa aacagtgttt tgagcaactt
     5821 gcggctagct tcctagtttg ctctttgatt ttcattgagt ataaggtatg atttgatttc
     5881 tttttgttga caaatatacc atattaaaaa gatatataag taattaactg agcttatctg
     5941 tcttgtcatc tctattaagg atggtttaga taatcgggtg tctgcttcta ggctagcacc
     6001 tcaatatcca aaggagtgat gaatttgaag gatataagaa atacctatct ctcagatgat
     6061 ttattgagga agaaagatag gagtttttga gctagtgaag gcttggattt ctaaaggtta
     6121 gaactatcat cttcagttct taaatcgaag aaataagcta tcttacggaa atagagaagc
     6181 attttttaag aacttgaata atttcgcacc ttaagagggt aataatacag tagttttatt
     6241 agcaaatatt tatggtgtag aggctag
//
LOCUS       DAVLVF010000095         6153 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVLVF010000095 DAVLVF010000000
VERSION     DAVLVF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.095"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(104..379)
                     /locus_tag="VYN66_001760"
     CDS             complement(104..379)
                     /locus_tag="VYN66_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV1495744.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(486..1325)
                     /locus_tag="VYN66_001761"
     CDS             complement(486..1325)
                     /locus_tag="VYN66_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1495745.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1467..2315
                     /locus_tag="VYN66_001762"
     CDS             1467..2315
                     /locus_tag="VYN66_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1495746.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2408..3325)
                     /locus_tag="VYN66_001763"
     CDS             complement(2408..3325)
                     /locus_tag="VYN66_001763"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV1495747.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3762..3967
                     /locus_tag="VYN66_001764"
                     /pseudo
     CDS             3762..3967
                     /locus_tag="VYN66_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4278..4571)
                     /gene="rpmA"
                     /locus_tag="VYN66_001765"
     CDS             complement(4278..4571)
                     /gene="rpmA"
                     /locus_tag="VYN66_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV1495748.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4588..4932)
                     /locus_tag="VYN66_001766"
     CDS             complement(4588..4932)
                     /locus_tag="VYN66_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV1495749.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(4948..5262)
                     /gene="rplU"
                     /locus_tag="VYN66_001767"
     CDS             complement(4948..5262)
                     /gene="rplU"
                     /locus_tag="VYN66_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV1495750.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5298..5385)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5444..6148)
                     /locus_tag="VYN66_001768"
     CDS             complement(5444..6148)
                     /locus_tag="VYN66_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV1495751.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
BASE COUNT         1889 a         1266 c         1032 g         1966 t
ORIGIN      
        1 cggaacattc aactttttaa ctctatttat tactagattt cataattaaa aagcctactg
       61 accaagctag aaagcttgat acaataggct ttttaaagac tgattattta acagcgtctt
      121 taagagcttt accagctttg aatgctggta ctttagaagc tgcaattgtc atttctttac
      181 cagtttgtgg gttgcgacct ttacgttctg cgcgctcacg aacttcaaag ttaccaaaac
      241 cgatcaactg aactttttca ccagctgcaa gatagtcagc tactgctgca aatacagctt
      301 caactgctgc tgctgagtct ttcttagtca attctgtagc ttctgctact ttagcgatca
      361 aatcttgttt gtttgccatg ttaatgattc ctccaaataa tttctaatta acaaatataa
      421 tcatatccta aaatcccttt taggtcaagt taaaaacgct atttcttccc attttattta
      481 tttttttagg agtggtaacg tatcaaaata gcccaagcgt tctcacccgt gtgagtttga
      541 ataatggaac ccgtttccaa aacagaaatt ggcttttcaa cataagcttg taagctttct
      601 ttcatctctt ttgcccaatc atcactacca gaatatgaaa ttccaatctc tgctacagca
      661 cgttcagaaa gcgatgttat caactcatct aaccattttt taaatgtttt agttccacga
      721 cctttaacca ttggctgcaa ttcatggtct ttcatttgca tgacaacacg gatattgaga
      781 agagagctca acaagccagt tacacggcta attcgtccac ctttgacaag attttccaaa
      841 gttgaaacac caatataaag ctctgtatgg tttttaacct cttctacatg agataaaatt
      901 gcctccatat ctttaccttc ttgagctaac ttcgcagcct caacaacttg gaatttcagg
      961 gcttggtcag tgaaggaact atcaacaaca gtcacatctg cagtagatag gctagcacct
     1021 tggcgtgctg cttctaccgt acccgaaaga gcatgggaca tatgaatagc aagaatctgg
     1081 ccaccatctt tgcataggtc ttcaaaaatc tcagcaaaga cacctacagg tggctgactt
     1141 gttttcggaa gattcttact ttcttgcatc aactgaagaa atttaccttc ttctttcaaa
     1201 tccgcatcag aataaacaac attatcaatc attacagata atggaacaat tgtaatatct
     1261 aattgcttta ctagttcagg ttcaatagta acagatgaat cggttacaat cttaattttt
     1321 gtcatagtat caatctttct attttaggat tcagattggt ttccttactt ctaattatat
     1381 caaaaaaaag attaaaaatc ctaatggagt caatcaaatt ttccgtaaaa tttgatataa
     1441 tcaacttata agaaaagagg tgtcctatga ttaaaaaaat ttaccccatt tttaccattt
     1501 tactaggtgc tgctatttat gcttttggac tgacttattt tgtagttccc catcatctct
     1561 ttgaaggagg ggcgacaggc attaccctca tcacctttta tctttttaaa atccctgttt
     1621 ccctcatgaa cctgctgatt aatattcccc ttttcatcct agcttggaag atctttggag
     1681 ccaaatccct ctattctagt ttactaggaa ccttagcttt gtccgcctgg ttagcttttt
     1741 ttgagcatat tccccttcat attgatcttc aaggtgattt actaatcaca gcccttatag
     1801 cgggaatcct attgggaatt ggccttggaa ttatttttaa tgctggaggt acaactggcg
     1861 gaactgatat tctagctcgt attctcaaca aatacactca tatatccata ggaaaactgc
     1921 tctttatctt agatttttgt attctcatgt tgattctcct aatcttcaag gatttgagat
     1981 tggtttccta cacgcttttg tttgatttta ttgtttctcg tgttattgat ttgattggtg
     2041 aaggaggata tgccggcaaa ggctttatga ttatcacaaa acgtcctgac caacttgcta
     2101 aggcgattaa tgatgacctc ggaagaggtg ttacttttat ttctggtcaa ggctactata
     2161 gtaaagaaaa tttgaaaatc atctactgta ttgtcggaag aaatgaaatt gtgaaaacga
     2221 aggaaatgat tcatcgaatc gatcctcaag cctttataac tattacagaa gcccatgaaa
     2281 tcctaggaga aggcttcacc tttgaaaaag aataaaaaga ggtaatgtcg tgacctcaaa
     2341 agttagacta aatcatctat cttttgggtt acagacaacc tcttttttgt tttatttact
     2401 caagctctta agaccaattc cgagttactt cttcatcagc ctttaactga tccactaatt
     2461 ggtcaactga gtcaaatttg gtcatatcac gaatgcgatc aagccaataa accatgacgg
     2521 tttccccata aatatcttga ttaaaatcaa aaatattgac ttcaaaacgt gcttcttctc
     2581 catcaaaggt cacatttttc ccgacactag ccatagcacg atacttctgt ctttgaatct
     2641 caacatcaac gacataaacg ccatctgctg gcatataagt acggtctaaa agcactaaat
     2701 tcgctgtcgg ataaccaatt gtacgaccac gagcattacc atgaaccacc atacctcttg
     2761 atggaagcgg tgcccccaaa agttttcctg cttctttcac atttccatct aaaatagctt
     2821 gacggatacg agttgaacta atctttcctt tctcatcttc tacaggtgga acaatgataa
     2881 cttctccatc aaagtaatcc tttaaatctt ctgctgtttt tttgtcagaa ccaaatgtat
     2941 aatcaaaacc tgcaacaata attttggcat tcatagcctt gatataagtt gcaaagaatt
     3001 cttgtgcagt gagactagcg aattgactac taaaatcaag gagatataat tcttctacac
     3061 cttcgcgctt taattttctt tcacgttcag cagggttcaa aatatgcaaa aacaaatctg
     3121 gatgataagg ctctaaagcg atctttggag attcattaaa ggtcataacg acgataggca
     3181 ataaatcctt tctcgcagcc ttgttggcaa cacgaaataa ttcttgatgc cccttatgta
     3241 tgccatcaaa atagccgaga acaacgactg aatcagatgg tgtgccaata tctttttggt
     3301 tttttatagg aatagtaata atcataaaat aattatatca tagcgatagc tatttctgga
     3361 acagaaaatc tgaaatgttg tttttttcac atgaagtgta cctgttttca aaaagcactt
     3421 tattctatcg ttgcttaact atgaactttg caatattctt ctcaaaaact tgtaggacat
     3481 cttcaaaatt ttgcaaggag tgattagact tgttcggtaa ccataaagtg tcatactatg
     3541 ttatgtatga aaaagcaatg caactaactc ctgagaactt taaattacta attggtgcca
     3601 aaaaggtaga atttagaatc gaggtacacc tatggctgta aaatttacaa aatgagacaa
     3661 cttgggcaag atgtttgaag aatttcctaa actccctgat ttgaagcaag tcactttccc
     3721 tgatgacaaa gaaaaaagcc aaaacagcaa agaaaaacta gatgactgct ttccaacaac
     3781 tcccatctag tgtgcttcag actgggctat ttttctctcc atctgttagc ttggattctc
     3841 agaccgtttc agctaaagaa tatcttttcc cttatcagaa ggaacggctc aaaccattca
     3901 gacaagtgaa gggacgacaa gccaatattt gaaaccagat agcagttctt atagtcaatt
     3961 gaaataaaat ctgaagaaat cgagtaggaa actcatatca atttttaaca gtgttctatt
     4021 ccagattcat actcaatgaa aattaaagtg caaactagga agttagccgc aggctgtact
     4081 tgagtacggc aaggtgaaat tgacgtggtt tgaatttgat tttcgaagag tatcaatcca
     4141 ctatacatat taaaaagtat gggtatcaaa ataaaaaaac aggctctccg aaaactcgga
     4201 aagccttatt ttaagctgtt tctagcttcc tcacctttac atttcaaggt tcgggataaa
     4261 aggttcaatg aaccttttta ttttgcgatt gggtaaacag aaacttgttt tttatcgcgt
     4321 cctttacgtt caaagcgtac tacgccttca actttagcga acaaagtatc gtctccacca
     4381 cgaccaacgt ttacacctgg atagatgtgt gtaccacgtt gacggtaaag gattgatcca
     4441 cctgttacag tttgtccgtc agctgcttta gctccaagac gttttgcttg tgaatcacgt
     4501 ccgtttgatg tagaacctcc accttttttg tgggcgaaaa gttgcaagtt gttaagagtc
     4561 atttttaaca taatgttttc ctccgtgtta gttttctgtg ataactctgg tttggacgaa
     4621 ctcagaatag ttctccgata agtttgccat acctaagaaa aatgattcaa agaataactg
     4681 ggtcatttct ctctggtgtg aaggaagatc ttttggtatt tcaaccatca gatagccacc
     4741 ttcatcttcg tttaattcta ggattggttc atagcctgca aatttctcaa tagaattgat
     4801 aaagttaatg gcaagcgtag aaaccgatgc acacacgaca tctaagccgt attcgccact
     4861 ctcggcgtgt ccagtaattt ccgcactcct cagctcgcca tcttcggctc tctcaaagac
     4921 tgcctgtatc atgtgttctc cttaaaatta agcgttgatt gcgttgatga caacttttgt
     4981 atatggttga cggtgacctt gtttacggtg gctacctttt ttaggtttgt acttgtaagt
     5041 aaccactttc ttttgttttc cttgtttttc aacagttcca actacagtag ctccagcaac
     5101 aagtggagtt ccgacaacag tgttttcacc accaacaaga acaacttcgt taaaagtaac
     5161 ttcttgacca gcttcaacgt tcaatttttc aacgtaaact gcttgaccaa cttcaacttt
     5221 aacttgtttt ccgccagttt tgataattgc gtatgtgctc attatgcacc tcctatgatt
     5281 tttatgggtt tccccgattt tttgtgaaga ctcgcctagc atcgtgggac gaaccactta
     5341 aacttgaata atcaagcaac gatgttcgtg cggttgcaca ggatcgtgca tagtcaactc
     5401 ttcaagtata gcatatctcc tattttctta caagtaataa cacctaaaat gaagcttttt
     5461 cttttacttt tttctgccaa gaggcaaaaa gcatgctgag gtaaaaaacg ctcatcataa
     5521 taggaacacc aagaatggtc ttttcatgat agaaaatcgt caaataggct gaaaagacaa
     5581 cgccaaggac aaaactacta agcaggctaa caaatatgaa tccttcacgc aaaaaaggag
     5641 tgtgcttggt tcggaaataa tctccaaaag ccagcatggt ccgtttgata ttccctgtca
     5701 taaaagcgtt attataggca atacccgaca cttctccaaa agcagttgtc accagtccca
     5761 tacagaaggc caagggcggc actagataga tattatccac agtttgcggc acaaaagcaa
     5821 taatgattga taagattgcc aagggaatca aggacagaat aggttttttc acaattctca
     5881 atttttcctt ataaatcgtt aataaaaaga ctcccatcat aaacgctagc aaggtgagaa
     5941 ccttgtccct aacatccgaa acattatttt taattaattc tactgaaaga aagacaacat
     6001 ttccagtttg tccagctaca agggtattcc cgcgaacaat aaaagtgtaa gcatccacat
     6061 atccagcaca aaacgtcaaa aaaagtgcta accttttaga ctgacgtgat atttttctta
     6121 taggtaataa cctcatttta cctcccattg tat
//
LOCUS       DAVLVF010000096         5937 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVLVF010000096 DAVLVF010000000
VERSION     DAVLVF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.096"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            64..831
                     /locus_tag="VYN66_001769"
     CDS             64..831
                     /locus_tag="VYN66_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV1495752.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDSYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            916..1254
                     /locus_tag="VYN66_001770"
     CDS             916..1254
                     /locus_tag="VYN66_001770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495753.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKS"
     gene            1282..1596
                     /locus_tag="VYN66_001771"
     CDS             1282..1596
                     /locus_tag="VYN66_001771"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV1495754.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(1697..4249)
                     /locus_tag="VYN66_001772"
     CDS             complement(1697..4249)
                     /locus_tag="VYN66_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV1495755.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(4311..5933)
                     /locus_tag="VYN66_001773"
     CDS             complement(4311..5933)
                     /locus_tag="VYN66_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1495756.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEDDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         1992 a          980 c         1104 g         1861 t
ORIGIN      
        1 aaaggagaaa ctaagtttaa agaatggaaa gcaatttaaa aaaaaccaac ctttattatt
       61 gtcatgatcg ggatttctct tattccagat ctgtacaata tcatattttt gtcatcaatg
      121 tgggattcat atgggcaatt gtctgactta cctgtggcag ttgtaaataa tgataaagag
      181 gcttcctata atggtaatac tatggcaata ggaaaagaca tggtgtccaa tttaaaagaa
      241 aataaaacct tggattttca ttttgtagat gaagaggaag gaaagaaggg attggaagat
      301 ggcgattact atatggtagt gactttacca agtgatttat ctgaaaaaac aactacatta
      361 tccaatattc aatcgacagc agcttatcaa tcattgacaa gtgagcaaca aactgagata
      421 agtgattctg tatctcaaaa ttcaactgat agtattcaat cggctcagtc aattgtagct
      481 ttagtacaag atttacaggg aagtttagaa aacttacaaa atcaatcttc taatctttcg
      541 actttaaaaa atcaatctaa tcaagtatca cctattactt ctacttcttt gataggattg
      601 tcaagtggat taacagagat acaaggagat gttactagca aattagttcc tgccagtcag
      661 tcgattgcat caggtgtaaa cgcatatact acaggtgttg ataaagtttc tcagggcgca
      721 agtcaactaa gtgaaaaaaa tgccaccttg acaggtagtt tggataaact agtttcaggc
      781 tcaaacacct tgacacaaaa atcttctaga ttgacagcag gagttggtta attacaatca
      841 ggatctgggc aattagcaga caaatccagt cagttacttt caggtgcttc tccattagag
      901 aatagagcta ataaattggc agatggatct gggaaactag cagaaggtgg aacaaagtta
      961 acttctggat tggaagattt acagacagga cttgcttctt taggacaagg actaggtaat
     1021 gctagtgatc aactcaaatc agtatcaaca gaatctaaaa atgcagagat tttgtcaaat
     1081 ccactcaatc tttcaaaaac agacaatgat caagttcctg taaatggaat cgcaatagct
     1141 ccttatatga tatcagttgc tctttttgtt gcagcaatat caacaaatat gatatttgcg
     1201 aaattgcctt caggacgtca tccagagagc cgttgggctt ggttgaaatc ttgagctgaa
     1261 ataaatggta ttatagctgt tttggcagga attttggtat atggaggagt tcagcttatt
     1321 ggtttaactg ctaatcatga gatgagaata tttattctca tcatcctaac aagtttagta
     1381 ttcatgtcta tggtgaccac tttagcaacg tggaatagcc gtataggagc ttttttctca
     1441 cttattttgc ttttactaca gttagcatca agtgcaggta cttatccact tgctttgaca
     1501 aatgatttct ttagatctat taatccctgg ttaccaatga gctattcagt ttcgggatta
     1561 cgacaaacaa tctctatcaa caagtcattt tcctagctgt catactagtt ctatttacta
     1621 gtttaggtat gctagcctat caacataaga aaatggaaga agattaaaaa aatcgaccga
     1681 ttaactggtc gattttttat gccttagatg actttcgtct gtgattatag attccaaata
     1741 gtaagagaga agtaaaggaa cagattgctc cagtaataaa accattggga atgaaggaaa
     1801 gtgtaatagt tcctttcccc ttgggaatgt caactttcat aaatccagtt tgagcttgtt
     1861 taatttctat tttcttacca tcttggtagg cagaccaacc tttgtcataa ggaatggtga
     1921 agaaaataga tgtatcttgt tggacatcat atgtagcaaa aaccttgttt ttagaagttg
     1981 atactgtgac aggttgttct ttaatttttt gaattgcctc ggtgaaagtt ttggtatcta
     2041 aacgatagaa ggtaggagat tcaaatgata cttgtgaatt tccagggaaa ctaacattga
     2101 tattgaaagt ttttttctct ttagtatatc ctagattaaa gaaggagaag acattatcag
     2161 ttgtaaaagt ctttttttca ccatttacaa ggatgtcaac cttcttttgt ttatcgttag
     2221 aaaagtgaag gtttatgaaa gagagataaa cttggctgtt ttctggaact tcaatttgat
     2281 actggattgc tgcatcttca tttgaagaac ttgtgacact aatcaaatca ttagtatttt
     2341 ctattttttc tgttttttca taaggtattg gagaaaaata atcaaaattg acgttagcaa
     2401 gttgatttaa aaatgaggcc tgattatcca aggtatgttc attgaacttg acatcattgt
     2461 aaacagattg actagcaact gcaatcggaa gagagtattg attttcatat agggtaagat
     2521 tatctttttg atagatatct ttaaagccat acttatcaat aggactgtct gagatattgt
     2581 actggatacc aaataaacta tcagccaaaa tactattatt tgcatatcgg agattgagat
     2641 tagtcccaga ggatttaaaa ccaagtttat ctaaagtaga gcttgatgaa cgatttcgaa
     2701 cagatgaaaa ttgagagatt ccattgtagt tgaatttcat actgtcattt cctgtctgag
     2761 tttgtagttt ttcagtacga gtaaattgat ttccaatata tgttgagaaa gattccatag
     2821 ctgggatatc tcgactataa gcacttcgag aagcaaatcc ccattcctta gcaattccgt
     2881 ccatttgaga tgaagcattt aaactcattt caaccagtat aaataaagag attagaatgg
     2941 caaatagatt cacagatata aactttttga taactgcaag gagtaaaaga gaatagacaa
     3001 ccaaaaattc aagagtaagc agaatattca aatctgttaa aaaagaataa tgcgatttta
     3061 gatagatggt agctaaaaat cctgttacta caagaaaaag cgaaactaaa aaattccaga
     3121 ctttaagttc tttcagacgc tttaagactt ctgctgctgt gtaaattaac aaggtagaga
     3181 aaatccaagc atagcgatgt aaaaacatgt ttggagtatg catgccttgc caaaataagt
     3241 caagagcttc tatgtaaaag cttgcaatta gaaatgcaaa gaatattaca tatatgagtt
     3301 tcacgtgaaa cttaatagat ttcagcgtaa aaaataaaat ggtcaaaata aagggaaata
     3361 gtccaacaaa aatcattggg atggccccat actttgttgt gtcaaaggaa ccaatgaatt
     3421 gcttagcaaa gagatcaaga taccagctac tttcagtttg aaactttgta acttcagtca
     3481 atttttcccc atgtgtctgt aaatcaaata gagtgggaag agtcataatc aaactagcca
     3541 taccagctaa aaaggagata actatgaaat caagaacaga tgattttcga gtcttaaagt
     3601 cccacgaaat ttgacagaga taccagaaaa taagaaacaa tactgtcata tatccaaaat
     3661 aataattttg aataaataag attgacagac ttgtaaagta caataggagt ttcttttcag
     3721 ttatcagtag atgtaaacca gttataatta aaggaatcaa gataaaaaca tctagccagg
     3781 tttttatctc taattgactg acagtgaaac tcatcagagc ataggaagta gataaggcta
     3841 gttttaaagt ctgagggata gattgaaaca atttattcaa actaaaaaag gttgacagac
     3901 caatcaatcc aaattttaag agagtggtca gatagacagc atctggcata ttcgttagat
     3961 caaaaaagta aactagaggc gcgagaaaac tacccaagta ataactagat agggcataga
     4021 agtttagccc tagaccactt gtaaaggtgt aaaacagact actatttcca tgtaggatat
     4081 ttcgtaaggc tacatcaaaa ataacgtatt gatgaaagcc atctcctaat agaggagagt
     4141 tgtcgctatt ccagtagata ccttgagata gatatactcc agacataatc actacaggaa
     4201 tgatgaaaga aataaaatag gttcgatatg tttttaaaaa tgatttcatg ttacctcgta
     4261 gaatgataga aaactcagtt ggttaaccca actgagtttt gaagttttat ttagtctttc
     4321 caaagttctt taacttttgc ttgtacttct gcattttcta ggaattcatc gtaggtttca
     4381 tcgatacggt caatgacgcc atttttagat aagacaatga tatggttagc caaagtttga
     4441 ataaactcgt ggtcatggct ggcaaagatg attgattctt taaagttttt caatccatca
     4501 ttcaagcttg agatagattc caagtccaag tgatttgttg gatcatcaag tacaaggaca
     4561 tttgatttta agagcatgag ttttgaaagc atgacacgaa ctttttctcc ccctgacaag
     4621 acatttacag gtttgttaac ttcatctcca gagaagagca tacggccgag gaaaccacgt
     4681 aggaaagtat tgtcatcatc ttctttactt gcgaattgac gcaaccagtc aagaattgat
     4741 tctcctcctg caaaatcagc tgagttatct tttggtaggt aagattgact agttgtaact
     4801 ccccacttga cagttccttc atagtcaata tctcccatga ttgcacgaat taatgcagtc
     4861 gtttgaatat cattttgtcc aataagtgct gtcttatcat ctggacgcaa gatgaaacta
     4921 atattatcca agatagtttc accatcaatc tttacagtta gattttctac tgtcaagaga
     4981 tcattaccaa tctcacgttc cgctttaaag ttgataaatg gatatttacg actagatggt
     5041 acaatctctt ctagctcaat cttatcaagc attttcttac gtgatgttgc ctgccttgac
     5101 ttagaagcat tggcagagaa acgagcaacg aattcttgca attgtttaat tttttcttct
     5161 gctttagcat tacggtctgc tagcaattta gcagcaagct cagaagattc cttccagaag
     5221 tcgtagtttc cgacatagag tttgattttt ccaaagtcaa ggtcggccat gtgagtacaa
     5281 actttgttta agaagtgacg gtcgtgggat actacgataa ctgtgttatc aaagtcaatt
     5341 aagaagtctt ctaaccaagt aatcgattgg atatccaaac cgttagtagg ctcgtccaag
     5401 agaagaacat ctggtttacc aaaaagtgct ttggcgagga gaacctttac tttttcaccg
     5461 ttggccaatt cgctcatgtt ttggtagtgt aattcttctg gaatgtttag gttttgaagt
     5521 agttgagagg cttcactctc tgcttcccaa cctccaagtt cggcaaactc tccttcgagt
     5581 tcggcagcac gaaccccgtc ctcgtctgag aaatcttcct tcatgtagat agcatctttc
     5641 tctttcatga tgctataaag tttttcattt cccatgataa cgacatcaat ggcacgttca
     5701 tcttcgtagt caaagtgatt ttgacgaaga acagagagac gttcatctgg accaagagag
     5761 atgtgaccag tagtaggttc gatatctcca gctaaaattt ttaaaaaggt tgattttccg
     5821 gcaccattag caccgattaa tccgtaagta tttccttctg taaatttgat attgacatca
     5881 tcaaaaagtt tgcgatcact aaaacgtagt gaaacatcag atactgtaag caatatt
//
LOCUS       DAVLVF010000097         5858 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVLVF010000097 DAVLVF010000000
VERSION     DAVLVF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.097"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..427
                     /locus_tag="VYN66_001774"
                     /pseudo
     CDS             1..427
                     /locus_tag="VYN66_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            476..940
                     /locus_tag="VYN66_001775"
     CDS             476..940
                     /locus_tag="VYN66_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV1495757.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDRGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            1097..1522
                     /gene="lacA"
                     /locus_tag="VYN66_001776"
     CDS             1097..1522
                     /gene="lacA"
                     /locus_tag="VYN66_001776"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV1495758.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1553..2068
                     /gene="lacB"
                     /locus_tag="VYN66_001777"
     CDS             1553..2068
                     /gene="lacB"
                     /locus_tag="VYN66_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV1495759.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            2079..3008
                     /locus_tag="VYN66_001778"
     CDS             2079..3008
                     /locus_tag="VYN66_001778"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV1495760.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            3010..3990
                     /gene="lacD"
                     /locus_tag="VYN66_001779"
     CDS             3010..3990
                     /gene="lacD"
                     /locus_tag="VYN66_001779"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV1495761.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGETAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            4224..5060
                     /locus_tag="VYN66_001780"
     CDS             4224..5060
                     /locus_tag="VYN66_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1495762.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYCQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            5096..5413
                     /locus_tag="VYN66_001781"
     CDS             5096..5413
                     /locus_tag="VYN66_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1495763.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     DAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5415..>5858
                     /locus_tag="VYN66_001782"
     CDS             5415..>5858
                     /locus_tag="VYN66_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1495764.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFI"
BASE COUNT         1922 a          984 c         1230 g         1722 t
ORIGIN      
        1 aattagcgaa tgattctggt aaaaagaaat gccacgctat gaaggctcaa gcgattgtca
       61 caagtcaagg gagattgttt ctttggatat cactgtgaac tattgtcatg atatgaagtt
      121 gttcaaaatg agtcgcagaa atatcggaca agctggtaaa atcttggctg acagtggtta
      181 tcaagggctc atgaagatat atcctcaagt acaaactcca cgtaaatcca gcaaactcaa
      241 gccgctaaca gttgaagata aaacctataa ccatgcgcta tctaaggaga gaagcaaggt
      301 tgagaacatc tttgccaaag taaaaacgtt taaaatgatt tcaacaacct atcgaaatca
      361 tcgtaaacgc ttcggattac gaatgaattt gattgctggc attatcaatc atgaactagg
      421 attctagttt tgcaggaagt ctaataaaga aactagcaag gtcaggcctt actttattgg
      481 tggacatcca ggatttaatt gtcctctact tgatgatgaa gtctatgaag attattatct
      541 agagtttgag aaagaagaga cttgctctgt tccacgtcct ttcccagaaa caggtatgtt
      601 ggatttccaa gatagaagtc catggctaga gggacaaaaa gaaatagatc ttagttatga
      661 tcttttcagc aaagatgcag tgactttgga tgaattgcaa tctcgaacaa ttgcccttcg
      721 ttctcttaaa catgataggg gattgaaagt gcattttgca gaatttccaa acctcatcat
      781 ctggtcaact ttgaacaagg gacctttcat tacctttgaa ccatggtctg gcttgtcaac
      841 attccttgaa gaaggagatc atttagaaga taagaagaat gtttgtctct tagaagccaa
      901 tcaggttgaa gaattagggt ttgagataga agttttataa aaaagtaaaa aataaataac
      961 aaaaacaaac acaaactgtt gacttcttca aacaatagta gtatattatg tttgtgagga
     1021 ctaattgact gttttttaaa gacagccaat acactgctgg aatccagcat agaaaaaata
     1081 aaaaggagta tacaatatgt ctattgttat cggtgcagat gctgcaggtt tgagattgaa
     1141 agaagttgtg aaagacttct tagaaaaaga aaacttccac cttgtggatg ttacagctga
     1201 aggtcaagac tttgttgatg tgactctcgc tgttgctgca gaagtaaaca aagaagaaca
     1261 aaaccttggt atcgtgattg atgcttatgg agctggtcca tttatggttg caactaagat
     1321 caaaggaatg gttgctgcag aagtttctga tgaacgttca gcttatatga ctcgtggcca
     1381 caacaactca cgtatgatca ctatgggtgc acaacttgtt ggtgatgaat tggctaaaaa
     1441 catcgctaaa ggatttgtta atggtaaata cgacggtggt cgtcaccaaa tccgggttga
     1501 catgttgaac aaaatgggct aattagatta tagtaagaaa ggtaagttaa aaatgagaat
     1561 tgcaattgga tgtgaccaca tcgtaacaga tgaaaaaatg gcggtttcag aatttttgaa
     1621 atcaaaagga tatgaagtca ttgactttgg tacatatgac catacacgta ctcactaccc
     1681 aatctttggt aaaaaagtag gggaagctgt aactagcggt caagctgatc ttggagtatg
     1741 tatctgtggt actggtgttg gtatcaacaa cgctgtaaat aaagttccag gtgttcgttc
     1801 tgccttggtt cgtgatatga caacagccct ttatgctaaa gaacaattga acgctaacgt
     1861 tattggtttt ggtggtaaaa ttactggtga attgcttatg tgtgatatca tcgaagcttt
     1921 catccatgct gaatacaaac caactgaaga aaacaaaaaa ttgattgcga aaattgaaca
     1981 tgttgaaagt cacaatgctc aacaaacaga cgcaaacttc ttcactgaat tccttgaaaa
     2041 atgggatcgc ggagaatacc acgactaaga ggtaacctat gattttaaca gttacaatga
     2101 acccatccat tgatatttcc tatcccttgg atgagttgaa gattgatact gtcaatcgtg
     2161 tggtggatgt aaccaaaacg gctggtggta agggactcaa tgttacccga gtactttcag
     2221 aatttggcga ttctgttctt gctactggtt tagtgggtgg caaacttggt gagtttttgg
     2281 ttgaacatat cgataatcaa gtaaagaaag atttcttctc aattcaggga gaaactcgta
     2341 actgtatcgc tattctccac ggagacaacc aaacagaagt tcttgaaaaa ggtcctgaag
     2401 tattggaaca ggaaggtcaa gactttttgg aacatttcaa aaaactcttg gagtcagttg
     2461 aagtagtggc catctcaggt agtctgccag ctggccttcc agttgattac tatgcgagct
     2521 tggtagaact tgctaatcaa gctggcaagc ctgtagtctt ggactgctca ggtgcagcac
     2581 ttcaggctgt tcttgaatca ccccataaac caacagtcat caaaccaaat aatgaagaat
     2641 tgtctcagct tcttggaaga gaagtttctg aggatttgga tgaattaaaa gaagtacttc
     2701 aagaaccttt gtttgcaggg attgaatgga ttatcgtttc acttggtgcc aacggtactt
     2761 ttgccaaaca tggtgacact ttctacaagg tagatattcc tagaattcag gtggtaaatc
     2821 ctgttggatc tggagactct actgtggcag gaatttcttc aggacttctt cacaaagaat
     2881 cggatgcaga attactcatc aaggcaaatg tccttggtat gctcaatgct caagaaaaaa
     2941 tgactggtca tgtcaacatg gccaactatc aagttctata tgatcaatta atagtaaaag
     3001 aggtataaaa tggctttaac agaacaaaaa cgtgtacgct tagaaaaact ttctgatgaa
     3061 aatggtatca tctcagctct tgcatttgac caacgtggtg ctttgaaacg cctcatggct
     3121 caacaccaaa cagaagaacc aactgtggcc caaatggaag aacttaaagt cttggtagca
     3181 gatgaattga ctaaatacgc ttcatctatg cttcttgacc ctgagtatgg acttccagca
     3241 actaaagctc ttgatgaaaa agctggtctt ctccttgctt atgaaaaaac aggttatgac
     3301 acaacaagca caaaacgctt gccagactgc ttggatgtct ggtctgcaaa acgtattaaa
     3361 gaagaaggtg cagatgcagt taaattcttg ctttactatg atgtagatag ctcagacgaa
     3421 ctcaatcaag aaaaacaagc ctacatcgaa cgcatcggtt ctgagtgtgt ggctgaagat
     3481 atcccattct tccttgaaat tcttgcttac gatgaaaaaa ttgcggatgc aggttctgta
     3541 gaatacgcga aagtaaaacc acacaaagtt atcggcgcta tgaaagtctt ttcagaccca
     3601 cgctttaaca ttgatgtctt gaaagttgaa gttcctgtta acattaaata tgttgaaggc
     3661 ttcgctgaag gtgaagtagt ttatacacgt gaagaagcag cagccttctt caaagcgcaa
     3721 gatgaagcaa cgaacttgcc atacatctac ttgagtgctg gtgtatcagc taaactcttc
     3781 caagatactc ttgtatttgc tcatgaatca ggtgcaaact ttaacggagt tctttgtggc
     3841 cgtgcaacat gggctggatc agttgaagca tacatcaaag atggtgaaac agcagctcgc
     3901 gaatggcttc gcacaactgg atttgaaaac attgatgagc tcaacaaagt tcttcaaaca
     3961 acagcaactt catggaaaga acgtgtgtaa gaaagtcctc ctagtttagg aacatgaatc
     4021 taaaaaaatt taaaaaaagt tgtatgtaaa ggtttacaaa ataacttact tgtgctatac
     4081 ttaaatcaca agttaataca aggtgagtgt tactaagtaa tattaggcat gatcacaggt
     4141 gaattagaaa tcagctgatt ttctagttca tttgtggtca ttttttgtac tcatatacct
     4201 ttaagatata aaaggaggtt gacatgtatc gaattctaaa tccaatgaat cacaatgtct
     4261 cgcttgtcag aaatgataag ggagaagagg tgattgtaat tggtaagggg attgcattcg
     4321 gaaagaagaa gggggatttg attgctgaaa atcatgttga gaaaatcttt cggatgaaga
     4381 ccgaagagtc cagagaaaac tttatggctc ttctcaaaga tgttccgctt gattttatca
     4441 cagtgaccta tgaaatcatt gataagctat caaagaaata tcattatccg attcaagagt
     4501 atctctatgt aaccttgaca gatcatattt actgttctta tcaagctcta actcaaggaa
     4561 ggtacaagga tagtaatctg ccagatattt ctgctaagta tcctgtcgct tttcaaatcg
     4621 caaatgaagc ttttgaaatt tactgtcaga agctagcaga tcattttcct gaggacgaaa
     4681 ttattcggat tgcttatcat ttcattaatg ctgaaggtga aaatgaagtg gaacttgtgg
     4741 agtcgattga taagaggaaa gaaattctca ggaatgttga agaagtttta acggactatg
     4801 caattcaacg aactaaaaag aataaccatt tctatgatcg ctttatgatc catttgaatt
     4861 atttcttgga ttatttagac agatctagag atgataacca atcacttctg gatatggaag
     4921 atcatattaa acaatcctat ccaaaagcct tcgagattgg ttccaagatc tatgatgtga
     4981 ttacgcaaca tacgggtctt gatttgtata aaagtgaacg agtttatcta gttctacata
     5041 tccaacgttt attgtcataa aaatttattt aaaactatat aaggagaatt ctatcatgaa
     5101 tagagaagaa gtaacattgt taggttttga aatcgtagcc tatgctggcg atgctcgttc
     5161 aaaactattg gaagccttga aggctgctga agctggtgat tttgaaaaag cggacgctct
     5221 ggtagaggac gctggtagct gtattgcaga ggctcaccac gcgcaaacaa gtctattgac
     5281 taaggaagct tctggtgagg acttagcgta tagtgtgacc atgatgcatg gtcaagatca
     5341 cttgatgaca actatcttgt taaaagacct aatgcaccat ttaattgaac tttacaagag
     5401 aggagttaaa taaaatggat aagttaataa tttttattga gaaaggaaag cctttctttg
     5461 agaaattatc gagaaatata tacttaagag cgataaaaga tggttttatt tccagtatgc
     5521 cagcagtatt attctcaagt atttttatac taatagctgc tgtgcccaat atttttgggt
     5581 ttaaatggtc tgatgaacag ttagctttta ttttgaaacc ttacaactac tcaatgggga
     5641 ttttagctct cttagtagct gggacaactg caaaatcgtt aacagattca gttaatacac
     5701 gtagtatgga gaaaaccaac caaatcaact acatgtcaac ttttttagca gctgttgttg
     5761 gattgttaat actggcagct gatccaattg aaggtggatt tgctaatggt cttttaggta
     5821 ctagaggatt acttactgca tttttggcag cttttatt
//
LOCUS       DAVLVF010000098         5701 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVLVF010000098 DAVLVF010000000
VERSION     DAVLVF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.098"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            123..311
                     /locus_tag="VYN66_001783"
     CDS             123..311
                     /locus_tag="VYN66_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV1495765.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            373..1131
                     /locus_tag="VYN66_001784"
     CDS             373..1131
                     /locus_tag="VYN66_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1495766.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            1189..2043
                     /locus_tag="VYN66_001785"
     CDS             1189..2043
                     /locus_tag="VYN66_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1495767.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            2155..3039
                     /locus_tag="VYN66_001786"
     CDS             2155..3039
                     /locus_tag="VYN66_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1495768.1"
                     /translation="MKKAMVIITPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEAIHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKLKED"
     gene            3202..4941
                     /locus_tag="VYN66_001787"
     CDS             3202..4941
                     /locus_tag="VYN66_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEV1495769.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESASNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYA
                     SVIISHGKYSLQEINSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELD
                     NQLVISPDGFMIF"
     gene            5027..5344
                     /locus_tag="VYN66_001788"
     CDS             5027..5344
                     /locus_tag="VYN66_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495770.1"
                     /translation="MNCRGHETRQRIVRDFEVQPKVHIKLLANQQKHSDAVATIEDEY
                     YVFIAESKIDGKKEVIQCCMGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGS
                     GNL"
     gene            5390..5671
                     /locus_tag="VYN66_001789"
     CDS             5390..5671
                     /locus_tag="VYN66_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495771.1"
                     /translation="MWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
BASE COUNT         1915 a          847 c         1198 g         1741 t
ORIGIN      
        1 tttaatacca aagatttaaa gttactgtgt taaaaatcct ctccactttt aaactaatgt
       61 attataaaag ccagttgaca ctagtgttac acctgtgata gaattaactt gaggtgatga
      121 aaatggcaac cgataaaaat agaataatga ttagcttgga tgataaaaat ctagagaaac
      181 ttgaaaattt agtagaagat gctagagata gaaggggaat gcgactaaca aagtcccagg
      241 ttattgaatt actcctaaat accgttgatt atttcgatga tattatgggg gctatttact
      301 caaaaaaata agagcaaaat tgctattatt ttttacaaat tggtgtaaca ctagtgatat
      361 aggagtgatg aaatggttgt ttatattaga caaagtaaat taccaagtga ggtttcaatt
      421 aataaataca atgctcaagt aggtgcctac ttacaaggag aagaagttat tttatatcaa
      481 tctttttctg aaataaaaga gttaacaagt gaagatatag ttgtagatta tataatggag
      541 actagagcat tactaaaaat gatgggctta aacgttccgg ttcatgacta tcctattgag
      601 cttaaagagt tttatggtcg aaagatttat gctggtattt taggagagat tgtgaatata
      661 cctgataatt ggggaaaatt tattaagcct aaagctggtt caaaagtctt tactggaaga
      721 gttgttaatg gaacccatga tttaataggt attggtctac ctttcgacta tcctatatgg
      781 attagtgagg ttgtagaatt catagctgaa tggcgttgtt ttgtgttaga tggtcgcgta
      841 ttagatgttc gaccctatac aggtgattat catgcacaat ttgatgcaag tgtaattgat
      901 gaagcgatat catgttggaa agatgcgcca atagcttatg gactagatat cggtgttact
      961 cgcgatggca gaacacttgt tgttgaagta aatgatggtt atgcattggg aaattatggc
     1021 ctatctcctt taaaatcaat caattttcat agagctaggt ggaaagaaat ggtaaaaccc
     1081 tattttgaaa aaaatgaaat ttttaaaatc caacaggatg ttattttcta aaataaatat
     1141 ggtagataac caatataaaa agcatcaaaa tgaacaagga ggaatctgat gcttggagca
     1201 attattggag atattgtcgg ttctgtttac gaatggaaca atatcaaaac aaaggacttt
     1261 cctttatttc ggaaggactg cttttttaca gatgacacgg ttatgacctg tgctgttgca
     1321 gaagcaatta tgaatggtgg acaaaaagat gacttcattg acgcgatgaa gaaatatggc
     1381 agaatgtatc cgaatgctga ttacggtgct cggtttaatg catggctaaa cagcgataac
     1441 cgtgagcctt ataatagctt tgggaatgga tcagctatgc gtatttctcc atgtgcttgg
     1501 atcatggact gtggttttta tgcgaaaact ggtatatggc catcatctag aggacttacg
     1561 agtctttctg cagaggtaac tcataatcat ccagaaggtg tcaaaggcgc tatggctaca
     1621 gctgatgcta tctttctatg tcgtttttac tttggtggtt attgtagaga gtacgagcaa
     1681 tcaattaacg acaatcctac agagtgtaaa agacgaatta aggattatat agaaaaagaa
     1741 tacgattaca atctatctca aactttagat gaaatccgtc ctaactatcg ttttaacgaa
     1801 acatgtcagg aaactgtccc tcaagccatt atcgcctttc ttgaaagtag agacttcgaa
     1861 gatgcgataa gaaatgccat ctcacttggt ggcgacagtg atactctcgc agcaatcact
     1921 tgtagcatag cagaggcagc ttacggtatt cctgattgga tcaaggataa ggcctattct
     1981 tacttggatg aacccttaaa ggatgtagtt aggcgatggg aaaatagaat aaaagcgtat
     2041 taatctccag tacgtcagat ttctcttttc tgtattttca ttgtataata ggattacatt
     2101 tacttgcata cgacgaaatg taaacatttc tgtttaaaat taaggataaa atcaatgaaa
     2161 aaagcaatgg taattatcac ccctacttct ggtggcgaga aggctttgga ttacaaagaa
     2221 aagctggaga ataaagcaaa agaatacttt gaatatgttg aaaccaaaat taccgaaaaa
     2281 gcgctggatg caacacattt tgctgaagaa gcttctcgtg agcagtatga tgcagtggtt
     2341 gtgtttggtg gagatggaac tgtcaatgaa gtcatttcag gtattgatga gagagactac
     2401 attcctaagt tagggattat cccaggcggt acgggtaacc tcattacaaa acttttggaa
     2461 atcaatcaag acatcgatgg cgcaattgac gaactggatt ttgatttaac caataagatt
     2521 gatatcggta aagcaaatga caactatttt ggttatatct ttagtatcgg ttctctgcct
     2581 gaggcgattc acaatgttga aatagaggac aaaacaaaat tcggtatttt aacctatgct
     2641 gtaaatacca tgaagtctgt catgacagat caggtcttta acattaaggt tgagacagaa
     2701 aatggaaatt atgttggtga agctatccat gttttggtcc ttttgacaaa ttacttcgct
     2761 gataagaaaa tctttgaaga aaacaaggac ggctatgcca acattttgat tctgaaagat
     2821 gcctctatat tctccaaatt atccgtcatt cctgatttat taaaagggga tgttgtcgca
     2881 aatgataata tcgagtatat caaagcgcgt aatattaaaa tctcttcaga tagtgaattg
     2941 gagtcagatg ttgacggaga taaatcagat aacctacctg tagaaatcaa agtcctagct
     3001 cagcgagtag aagtattttc aaaactgaaa gaggattagt atatagagaa agcctttttt
     3061 aaggcttttt gtatacttta aaagatagtt cctttaacaa cggacattcc ttgcaaatag
     3121 tttacaaaaa tagtatactg gatttattga gtttgaaaac gtttgcgtaa aatttgaatg
     3181 aatactttag gagacaaatt gatggaattg agtgctattt accataggcc tgagtcggag
     3241 tatgcctatc tttataagga taagaaactc catattcgaa ttcgaactaa gaaaggggac
     3301 attgaaagca tcaacttgca ctatggggac ccttttatct ttatggagga gttttatcag
     3361 gatacaaaag aaatggtcaa gataacttct ggtaccttat ttgaccattg gcaggttgaa
     3421 gtgtcagttg actttgcacg tatccagtat ctctttgagc tcagagatac agaaggtcaa
     3481 aatattttgt atggcgataa agggtgtgtg gaaaattctc tagaaaatct tcatgcaatt
     3541 gggaatggat ttaagttgcc ttatcttcat gagattgatg cctgcaaagt tcctgactgg
     3601 gtttcaaata cggtatggta tcagatattt cctgaaagat ttgccaatgg caatgctcta
     3661 ttaaacccag aagggacttt agactgggat tcatctgtca cacctaagag cgatgatttc
     3721 tttggtggtg atttacaggg gattattgat catatggatt acttgcaaga cttgggtatt
     3781 actggactat atctttgtcc catctttgaa tctgcaagca atcacaagta caatacgaca
     3841 gattactttg aaattgaccg tcattttgga gacaaggaga catttcggga actggtggat
     3901 caagcgcatc atcgtggcat gaaagtcatg ctggatgcgg tatttaatca tattggttcg
     3961 caatctcttc aatggaaaaa tgtcgtcaaa aatggtgaac agtctgctta taaggattgg
     4021 ttccatattc aacaattccc agtgacaact gaaaagctag ttaataagag agacttaccc
     4081 tatcatgttt ttggtttcga ggactatatg cctaagctaa atacagccaa tccagaggtc
     4141 aagaattatc ttttaaaggt tgcgacttat tggattgaag agtttaatat cgatgcttgg
     4201 cgtttggatg tggctaatga gattgaccat cagttctgga aggattttcg taaggcagtt
     4261 ttagctaaaa atcctgatct ttatatccta ggagaagtct ggcatacatc tcagccttgg
     4321 ctaaatggag atgagttcca tgccgtcatg aattatcctt tatctgatag tatcaaggac
     4381 tatttcttac gaggaattaa gaagacagac cagttcatcg atgaaatcaa tggagagtct
     4441 atgtattaca agcagcagat ttcagaggtc atgtttaatc tcttggattc acatgataca
     4501 gagcgaatcc tgtggacggc caatgaagat gttcaactgg ttaaatcagc cttagccttt
     4561 ctctttttac aaaaaggaac accgtgcatt tattacggaa ccgagctagc cttgactgga
     4621 ggaccagatc cagattgtcg tcgttgtatg ccttgggaac gtgtatcaag tgacaatgat
     4681 atgctgaact ttatgaagag gctgattaag attcggaaat acgcgtcagt aatcatttcg
     4741 catggcaagt atagccttca agaaatcaac tctgatctag tagctctgga atggaaatac
     4801 gaaggacgga tcctcaaagc aatattcaac caatcaacag aagattatct tttagagaaa
     4861 gaagcagtag cactagcaag caattgccaa gaattggata atcagcttgt catctctcca
     4921 gatggattta tgattttcta aaaactagtt gatgaagatt atggtacatt tcatacctta
     4981 tatagtataa taaggctagt tactaaactt gtaaaggaga acttaaatga attgtagagg
     5041 acatgaaaca agacaaagaa ttgttagaga ttttgaagtt cagcctaaag tacatattaa
     5101 gctgttagca aatcaacaaa aacatagtga tgcagtagca actattgaag atgaatatta
     5161 tgtatttatc gctgagagta aaattgatgg caagaaggaa gttattcagt gttgcatggg
     5221 tgcggcaagg gattttttag aactaattaa tcacaaaggg ctacctcttt ttaatccgct
     5281 tgtaggtgat tctcatgtaa ataatagaca agaatatgac aatacaggga gtggaaattt
     5341 ataacctgaa aagtggaatg aaactgcaaa gcagctttat aatgctataa tgtggttgat
     5401 tattttatgg aatgctaagc cggatacacc tttatttaat tttaaagacg aagtaattaa
     5461 gtataaaaca tatgagcctt ttgaaagcag tataaaaaga gtaaatacta ctataaagaa
     5521 tggtagtaaa gggaaaactc tgactgagat gattaatggc tacagagcgg ataacgatat
     5581 tagagatgaa atttgtaact ttaatattct gaaaaataaa attcgtgata tgaaagacca
     5641 acaaggaaat acaatggaat cttactttta gttattgttg aattttgggt attctataaa
     5701 a
//
LOCUS       DAVLVF010000099         5377 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVLVF010000099 DAVLVF010000000
VERSION     DAVLVF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.099"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            10..957
                     /locus_tag="VYN66_001790"
     CDS             10..957
                     /locus_tag="VYN66_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEV1495772.1"
                     /translation="MTEEKFQKIIQSKDTETLAFILEATPYHLSIDVLENPSQTEISL
                     MTKLVNDYRWAYAESPSDKIVTLFALRYVYHNIKVLLKSKAAIKKDFSKLLIPIGIFD
                     IESLKHLVSSLHSDTLPDFMVHEVESIWNEYETFNNIRVLDVGADLAYFKHLKLLSNE
                     LGEVLSQVIVAMIDFYNIITVKRGLSQNKSHGDILQLLSDEGSISAKEFIYIVENQEI
                     FVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDLLLYKTLDQGRYNVDGPLVLA
                     RYLLGCEFEVKNLRMIISALQNTIPFESIKERIRPHYGS"
     gene            947..1267
                     /locus_tag="VYN66_001791"
     CDS             947..1267
                     /locus_tag="VYN66_001791"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEV1495773.1"
                     /translation="MEANKYKIGIIGSHDIILPFSMIGFDIFSAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            1330..3105
                     /locus_tag="VYN66_001792"
     CDS             1330..3105
                     /locus_tag="VYN66_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEV1495774.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     AFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            3106..4491
                     /locus_tag="VYN66_001793"
     CDS             3106..4491
                     /locus_tag="VYN66_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEV1495775.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            4514..5125
                     /locus_tag="VYN66_001794"
     CDS             4514..5125
                     /locus_tag="VYN66_001794"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEV1495776.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIYKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         1775 a          764 c         1106 g         1732 t
ORIGIN      
        1 aacgatttta ttacagaaga aaaatttcaa aaaattatac aatccaaaga tacggagaca
       61 ttggcattta tcttagaagc aactccctat catttatcga ttgacgtttt agaaaatcct
      121 agtcagacag agatttcgct aatgacaaaa ttagtcaatg attatagatg ggcctatgct
      181 gaaagtccgt ctgataaaat tgtgacttta tttgctttac gatatgttta tcataatatc
      241 aaagttttat taaaatctaa ggcggcaatt aagaaagatt tttctaaatt attaattcca
      301 atagggattt ttgatataga aagtttaaaa catttagttt cttccttaca ttcagataca
      361 cttcctgatt ttatggttca tgaagtagaa tcaatttgga atgagtatga aacttttaat
      421 aatattcgtg tacttgatgt cggagctgat ctagcatatt ttaaacatct gaaactttta
      481 tctaatgagt taggtgaggt gctgtctcag gttattgttg caatgattga cttttataat
      541 attattactg taaaacgtgg tttatctcaa aataagagtc atggggatat tttacaatta
      601 ctttcagatg aaggaagtat ttctgctaaa gaatttatat acattgtaga aaatcaagaa
      661 atatttgtgt ggttcaataa aataaatcca agcttagatt caatcttttc aacttacgaa
      721 ttgaagatgc aggacgcaac aatttcatct tctgagttag aatttttatg tgatttacta
      781 ttgtataaaa ctttagatca aggaaggtat aatgtagatg ggccgttagt tcttgctaga
      841 tatttattgg gatgtgagtt tgaagtaaag aatcttagaa tgatcatatc agctcttcaa
      901 aatacaattc cttttgaatc aataaaagaa aggatacgcc cgcattatgg aagctaataa
      961 gtataaaatt ggcataattg gtagccatga tattatttta ccctttagca tgattgggtt
     1021 tgatatattt tctgcctatc aagaacaaga agctataaat actctaagaa aattagctca
     1081 atctgattat ggtgtcattt atatcactga agacattgct tcaatgatat tagatacaat
     1141 tcgccattat gattctcaag ttgtgcctgc tattatttta ttaccgactc ataaacaagg
     1201 tttaaattta ggattaaaac gtatagagga taatgtagag aaagcagtag gacacaatat
     1261 tttataataa tgtacaaaat tgtctgtaat attattctat aatttttgga cttagtaagg
     1321 agaataactt tgactcaagg gaagattata aaagtatcgg gacctctagt tattgcatca
     1381 ggtatgcagg aggctaatat tcaagatatt tgccgtgtag gtaagctagg gttaatcggt
     1441 gaaattattg aaatgagaag agatcaggca tctatccaag tctatgaaga aacatctggt
     1501 cttggtccgg gagaacctgt tgttacaact ggagaacctc tctcggttga attagggcca
     1561 ggattgattt ctcaaatgtt tgatggcata caacgcccat tagatcgatt taaattggct
     1621 actcataatg attttctagt tcgtggggta gaagttccaa gtttggatag agatattaag
     1681 tggcattttg attccactat agcaattggt caaaaagtga gtacgggtga tattcttgga
     1741 actgtcaagg aaaccgaggt agttaatcat aaaattatgg ttccttatgg agtatctgga
     1801 gaagtcgttt ctattgcatc tggcgatttt acaattgatg aagttgtata tgaaataaaa
     1861 aaattggacg gtagtttcta taaaggaacg cttatgcaaa aatggcctgt ccgcaaggcg
     1921 cgtcctgttt ctaaacgttt aattccagaa gaaccattaa tcacaggtca acgagttatt
     1981 gatgcattct ttccagtaac caaaggggga gctgcagcag ttcctggacc gtttggagca
     2041 ggaaagacag ttgtacaaca ccaagtagct aaatttgcca atgttgatat tgttatttat
     2101 gtcggttgtg gagaacgtgg aaatgaaatg acggatgtac tgaatgagtt tcctgagttg
     2161 attgacccta ataccggaca atcaattatg caacggacag ttctgattgc taatacttca
     2221 aatatgcctg ttgctgctcg tgaggcttca atttatacag gaattaccat ggctgagtat
     2281 tttcgtgata tgggctactc tgtcgccatt atggctgatt caacttcacg ttgggcagaa
     2341 gcgctacgtg aaatgtcagg acgtctagaa gaaatgcctg gtgatgaggg ttatcctgct
     2401 tatctgggaa gtcgtatcgc tgaatattat gaaagagcag gacgttctca ggttctaggg
     2461 cttccagaac gtgaaggaac gattactgct attggagctg tatcgccacc tggtggagat
     2521 atttcagaac cagttactca aaacacttta cggattgtga aagttttttg ggggcttgat
     2581 gctccgttgg cacagcgacg tcattttcct gcaattaact ggcttacatc ttattcacta
     2641 tataaagaca gtgtgggcac ttatatagat ggtaaagaga agacagattg gaatagtaaa
     2701 ataactcgtg cgatgaacta cttacaacgg gaatctagtt tagaggaaat tgttcgtctt
     2761 gttggaattg attctctgtc tgataatgaa cgactaacga tggaaattgc taaacaaatt
     2821 cgagaagatt atttgcaaca gaacgctttt gattcggtag atacattcac ttcgtttgca
     2881 aaacaagaag caatgctaag taatattctc acttttgctg atcaggcaaa tcatgcttta
     2941 gagttgggtt cttactttac agagattatg gaaggtaccg tggcagttcg agaccgtatg
     3001 gcgagaagta aatatgtttc agaagataga ttagatgaaa tcaaaattat atcaaatgag
     3061 attacacatc aaattcattt gatattagaa acaggaggtc tataaatgag tgttataaaa
     3121 gaatacagaa ctgctagtga agttgttggg cctcttatga ttgttgaaca agtaaataat
     3181 gtgtcttaca atgagttagt tgaaattcaa cttcataatg gagaaattcg tcgtggacaa
     3241 gttttagaga tccacgaaga taaagcaatg gttcagcttt ttgaaggatc tagtggaata
     3301 aatttagaaa agtctaaaat tcgttttgct ggtcatgcat tagaattggc tgtatctgag
     3361 gatatggttg gtcgtatttt taatgggatg ggaaaaccaa ttgatggtgg accagattta
     3421 attccagaga aatatttaga tattgatggt caagctatta atcctgtatc tagagattat
     3481 ccagatgaat ttattcagac agggatctcc tctattgatc atttgaatac tcttgtacgt
     3541 ggtcaaaaat taccagtatt ttcaggttcg ggcttacctc ataatgaatt agctgctcag
     3601 atagcaagac aagcgactgt tttaaattct gatgaaaatt ttgcggttgt atttgcagca
     3661 atgggtatta cttttgaaga agctgagttt tttatggaag aactcagaaa aacaggagcg
     3721 atcgatcgtt cggttttatt tatgaacttg gcaaatgatc ctgcaattga gcgtattgca
     3781 actccccgca ttgctttaac tgcggcagag tatctagctt ttgaaaaaga tatgcacgtt
     3841 ctagttatca tgacggatat gactaactat tgtgaagcgt tacgtgaagt ctcggcagct
     3901 cgccgtgaag ttccagggag acgaggctat ccgggatatt tatatacaaa tttatcaact
     3961 ctatacgaaa gggctggtcg cttagttggt aaaaaaggtt cggtgacaca gattcctatt
     4021 ttaacaatgc cagaagatga cataacacat ccaattcctg atttaactgg atacattact
     4081 gaagggcaaa ttattttgtc gcatgagttg tataatcaag gttatcgtcc accaatcaat
     4141 gttttacctt ctctctctcg attaaaagat aagggatctg gagaaggtaa aactcgtgga
     4201 gatcatgctc caactatgaa tcaactgttt gcagcctatg cccaagggaa aaaggttgaa
     4261 gagttagcag tagtattagg agaatcggct ttatctgatg tagataaatt gtatgtgagg
     4321 tttacaaagc gttttgaaga agagtacata aaccaaggat tttataaaaa tcgaaatata
     4381 gaagatacgt tgaatcttgg gtgggaatta ctatcaattc ttcctagaac agagttaaaa
     4441 cgtatcaaag atgatttgct tgataaatac ttacctttgg tagaagttta atccggaaat
     4501 ggagtgatta tctatggtac gtttgaatgt aaaaccaact cgtatggaat tgaataactt
     4561 aaaggaacgt ttgacaacag ctgaacgtgg acataagtta ttaaaggata aaagagatga
     4621 attgatgagg cgatttattt ctttgattcg tgagaataat caacttcgga aagaagtgga
     4681 aagttatcta attgataatc taaaatcctt tgcagttgct aaatcattaa agaattctca
     4741 aatggtggag gaattatttt caattccatc gaaagaaatt gaattatttg ttgagaaaga
     4801 aaatatcatg agtgtaacag ttcctagaat gcatatgaat attacttctc aaaatgagaa
     4861 cagtgaatac agctatttat cttctaatag tgaaatggat gatgtatttg ctacaatgaa
     4921 tagtttaatt tataaattac taagactggc agaagttgaa aaaacgtgtc agttaatggc
     4981 tgatgaaata gaaaaaacac gtagacgtgt aaatggttta gaatactcga ttattccaaa
     5041 cttgtcggaa actattcatt atatagaatt gaaactagag gaggcagaaa gagccaattt
     5101 agttcgtatt atgaaagtga agtagatcct ttatttagat tattaattag atgaacaaat
     5161 atcagcttgg ataaggcttt aagcctttct aagctttttt tattgacagt atcaggatat
     5221 ctttttcaaa attttggttt gttagataat gaaaatgttt ctactaatct agatttagga
     5281 ttagtaaatc gtaaatgtaa ttatatagaa agtaagcgcg tcataacaag gtatctatca
     5341 ttcatggagc tcctcctgta tactattagt aaagtaa
//
LOCUS       DAVLVF010000100         4920 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVLVF010000100 DAVLVF010000000
VERSION     DAVLVF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.100"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(145..2034)
                     /locus_tag="VYN66_001795"
     CDS             complement(145..2034)
                     /locus_tag="VYN66_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV1495777.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNFFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2172..3737
                     /locus_tag="VYN66_001796"
     CDS             2172..3737
                     /locus_tag="VYN66_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1495778.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3737..4477
                     /locus_tag="VYN66_001797"
     CDS             3737..4477
                     /locus_tag="VYN66_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1495779.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4586..4801)
                     /locus_tag="VYN66_001798"
     CDS             complement(4586..4801)
                     /locus_tag="VYN66_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495780.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
BASE COUNT         1520 a         1127 c          884 g         1389 t
ORIGIN      
        1 ggctttttgc cgtgctctaa aatcatcgcc aaatcatcaa attgaaataa gaaatagttt
       61 agataaaact attgactttt cttatagttt gtctttcttc tatcttccat tatatcatac
      121 tttttcagtc tatggtagct ctctttaaaa ggctccgata ctgccgccac ctccgcctcc
      181 agagaagcca ccgccagaac ttccacttcc agaagatacg gagtaggtac ttgctgtatt
      241 tgcgacacta gcataatggc tcatttgcgc gcttgaatga taaaacatac tgtgccagcc
      301 ataagctaca tagagattga tatctggatt ttcaacttga atctgatgaa ccttcatcaa
      361 atgactaacc ttgtccgcat agccaaataa ggttgcatag accaagagac gattccaaac
      421 aacaatactt tccaattcag ccttatccag tcgtgcaatc tcacgtaaca tattttcaaa
      481 actggtccag agatagtaga cctcagctcc cgcttcattt agaacaccat cacgattatc
      541 tagtcgaagc ttccaataat agaaaacaga caaaactaac cctagaaaac caagtattgg
      601 caaagggagg taaagatagc catgaacgtc taaactgtac aagaacaaac caaatccgat
      661 aaataggggc aagatagtca agacacccat acccacttgc aaagtctttt ccaaaccagt
      721 taaaggacga tagtaatctg ggagccccca gaaggaaact cgttttctca ctccttcttg
      781 catctggttc aatacctctt caaaagaaga tttgagttga agccctcttg cttgaatccg
      841 tttttcatca gaaactttgg ctctacgata aagactatca gataccttgt aatccgcaaa
      901 aaaattggaa agagtggctt cttttttgcc tgaaaaggct agatttagac agtctctctc
      961 aaatcttgac aaaccatctt ctttcaccaa tctcaagcca actgcatctc cttctgaaat
     1021 aatagagaca ttcccacggt ctatcacatc tagcaaggta gcttgaataa gttggtcaaa
     1081 agtgaatttg ccagccccct tgaccaaggg actcacttcc tccaaggagg tcgagtagac
     1141 tgcttctgat aaaaccatag gctctaattc cattggtggt tcatagagac gatgattttt
     1201 ggcatatttg actgaaggag tggtctttct tctataaata aaatagaagc agacactcaa
     1261 taacaaggag atagaaagta tcgaagggag gacccaagta acgagttgtt tactctgatc
     1321 tttttctcta acaatcgagt cttctatctt attaaactct tctaaacgat tccctttcaa
     1381 gccctgatcc ctagcgctag caaaatcggt ccgagaccaa taggcatgca actcaactcc
     1441 acgcttagcc ggaagattgt ctaaacggat agtataatca aggttactct tttcaatcgt
     1501 tccctctcta aaaagtttcc ctgtatggaa aaagagtttc tcagccccct tgtctcccct
     1561 tacatgaaat tcaaactttt caatagactc tgaactatct gtcagaggtt gccaatttaa
     1621 ttcagcgata tcatcataaa ggaaaagtaa attttttaag ttccagacga ggtcaacttc
     1681 aactatgtcg ccctcctgac ctggattata gactctcaca gtataaccat ccgcttcttc
     1741 tgttacttcg ctagtcacat ctgctagttc tgcaccgttt ttcgcagcct gaacctttgg
     1801 atgagggtca atgtcaaacc cgctaggcat cttaccagca cgtccaagtc ccacgatttg
     1861 gcccttaaag tcctcctcaa actggtaaac tatcttctgt ctaaactctg ccgtattatc
     1921 tgcatgaata tacaaatccc cttgataaga gtttatcttg aaatcaatgg caaaaacaga
     1981 gagtggaaga aggcaaaaca agcctaacac cagtaagaaa aaagtttttt tcatcaacta
     2041 agcccccttt ttatctttct tatcattata gcatcttttc gtaagtaaag gcttacttat
     2101 gttgaaaaat tacgcatttt tcgataaaat aggagacagt tattttttaa tagatcagaa
     2161 taggagaaat catgagaaaa atatacctat ctattttcac aagtctcttg ctgatgctag
     2221 gacttgtcaa tgttgctcaa gccgatgaat atttacgcat cgggatggaa gcagcatatg
     2281 ctccctttaa ctggacccag gatgatgata gcaacggagc tgtcaaaatc gatgggacca
     2341 atcagtatgc caacggatac gatgttcaaa tcgccaagaa aatcgctaag gacttaggta
     2401 aagaaccttt ggttgttaaa accaagtggg aaggtctagt ccctgccctt acttctggta
     2461 agattgacat gattatcgca ggtatgagtc caactgcaga acgcaaacaa gaaattgcct
     2521 tttcgagcag ttactacact agcgaaccag ttttgcttgt caaaaaagat tctgcctacg
     2581 caagtgctaa atctttggat gactttaatg gtgcgaaaat cacttctcaa caaggtgtct
     2641 acctctacga cttgattgca caaatcccag gtgctaaaaa agaaacagcc atgggagact
     2701 tcgctcaaat gcgccaagct cttgaggctg gtgtcattga tgcttatgtt tctgaacgtc
     2761 cagaagcact gactgctgaa gctgcgaact ctaagttcaa gatgattcaa gtagaacctg
     2821 gtttcaaaac tggggaagaa gatacagcta tcgctatcgg gcttcgtaaa aatgacaatc
     2881 gtattagcca aatcaatgcc agcattgaaa ccatttcaaa agatgaccaa gttgccttga
     2941 tggatcgtat gatcaaggaa caacctgccg aagctacaac aactgaagag actagcagta
     3001 gtttctttag ccaagtcgct aaaattcttt ctgaaaactg gcaacaactc ttgcgtggtg
     3061 ctggtatcac tcttttaatc tctatcgtcg gaaccatcat aggtctcatt attggacttg
     3121 ccattggtgt cttccgtact gctcctctct ctgaaaacaa agccatttat ggccttcaaa
     3181 aactagtcgg ctgggttctc aatgtctaca ttgaaatttt ccgtggtacg ccaatgattg
     3241 ttcaatcgat ggttatctac tatggaactg cccaagcttt cgggatcaac cttgaccgta
     3301 cactggctgc tatcttcatc gtttcaatca ataccggtgc ctacatgact gaaatcgtcc
     3361 gtggtggtat cctagcagtt gacaagggac aatttgaagc tgcgactgct cttggtatga
     3421 cccataacca gaccatgcgt aagattgtcc tacctcaggt agtccgtaac atcctacctg
     3481 caactggtaa tgaatttgtc atcaatatca aagatacatc tgtattgaac gttatctctg
     3541 ttgtcgaact ttatttctca ggaaataccg tggcaacaca aacctatcaa tacttccaga
     3601 catttacaat catcgccgtg atttactttg tcctcacctt caccgtaaca cgtatcctac
     3661 gatttatcga gcgccgaatg gacatggata cctatactac aggtgctaac caaatgcaaa
     3721 cggaggattt gaaataatga cacaagcaat ccttgaaatt aaacacctca aaaaatccta
     3781 tggacaaaac gaagtgctaa aagacatttc actcactgtc cacaagggag aggtcatctc
     3841 tatcatcgga agctctggaa gcggaaaatc gaccttccta cgctccatta acctacttga
     3901 aacaccaact gatggacaaa tcctttatca tggacaaaac gtcctcgaaa aaggctatga
     3961 cctcacgcaa taccgtgaaa agttggggat ggttttccaa tcctttaacc tctttgaaaa
     4021 tctcaatgtt cttgaaaaca caatcgtcgc tcagacaact gtcctaaaac gcgaacgcac
     4081 agaagctgaa aagattgcca aagaaaacct ggaaaaggtc ggcatgggag aacgctactg
     4141 gcaagccaaa ccaaaacaac tctcaggtgg tcaaaaacaa cgtgtggcca tcgctcgtgc
     4201 cctctccatg aatccggacg ctattctctt tgatgaacca acatcagctc tcgatccaga
     4261 aatggttgga gaagtcctca aaatcatgca ggacctggct caggaaggct tgaccatgat
     4321 tgtcgtaacc catgaaatgg aatttgcccg tgatgtctct caccgtgtta tctttatgga
     4381 taagggcgtg atcgctgaag aaggtaaacc agaagacctc ttcaccaatc ctaaagaaga
     4441 ccgaacaaaa gagttccttc aacgctatct caaataaaaa gaaaaggctg catcaaccgt
     4501 gcagtctttt tgctgtcctc aaaatgaaaa ggcagattct ctatctctac tacttgaagg
     4561 tcaagaatcc gccgttatcc aaagattaat cttcaaattt ttcataattt ttttcataga
     4621 aatcaattaa gcctagggct gtttcaaatg aaatattttt tacttttgca cgaccctgcg
     4681 ccagagcaat gatagacatt tcacgcgcat tcgtttcttt agagatacgg tagcctgtga
     4741 ttttcttatc acgaacccag ccaacaactg attctacttt ttcaaagttt gacttagcca
     4801 tgtttttctc ctattttctt ctttataata tttaattgta tacgttttta ctacttttgt
     4861 caataaaaaa aacatatatt caacgaaaat aattagttca gaattttctt acttcctttt
//
LOCUS       DAVLVF010000101         4841 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVLVF010000101 DAVLVF010000000
VERSION     DAVLVF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.101"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            61..225
                     /locus_tag="VYN66_001799"
     CDS             61..225
                     /locus_tag="VYN66_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV1495781.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKENKDKFLSILTSLAGLALV
                     LVAVWLGWPK"
     gene            304..1032
                     /gene="yaaA"
                     /locus_tag="VYN66_001800"
     CDS             304..1032
                     /gene="yaaA"
                     /locus_tag="VYN66_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV1495782.1"
                     /translation="MKILIPTAKEMNTDLPSIETTLLRSESQAVLDALALYSASQLES
                     FYKVSAEKAAEECQNIQALKRQTAQHYPALKLFDGLMYRHIKRDKLTEVEQAYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAAYDEAVKQEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(1083..2567)
                     /locus_tag="VYN66_001801"
     CDS             complement(1083..2567)
                     /locus_tag="VYN66_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1495783.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYISGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            2783..>4841
                     /locus_tag="VYN66_001802"
     CDS             2783..>4841
                     /locus_tag="VYN66_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1495784.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETK
                     PAVTTTERSAVDSAILPISTAAKVETTSAPASTNEILKPSLSLSDARQNPAIRKEDVD
                     RGYSGFRTAGSGFRAAGSGFRAAGPENKPILNPNNTIAFSDISQGLHSFRGIGHSRGG
                     REIHYDVTTVRRGNRLNFTIKYSGPGEFVNNNFILDKGDGFGNPSNATITSSNPRVRE
                     QSKSISQGANYVSHSGYSMTSATSTNTEQTIRFSLPIINPNGDLSVRLKPVTFNVDQG
                     GGGAATSNDPYSNSNYYHRANPLLLDANPYGGTNNKTVSEDIDFQTVYLPTSKLPEGQ
                     TRLVREGEKGQRQITYKVHRFGNETLLGLPISNRVTKEAKPRIMQIGVAKELIDTVKP
                     RVDQNKVGDTNNLTFYLDNDGNGVYTEGVDELVQKIAIKDGAKGEKGDQGERGLTGAK
                     GEKGDRGERGLTGAQGAKGEKGDRGERGLTGAQGAKGEKGARGERGLTGAQGAKGEKG
                     DRGE"
BASE COUNT         1641 a          796 c         1019 g         1385 t
ORIGIN      
        1 ggtttattta agaaatatat gttatagtaa aataaattaa gactaatgga ggacaaagaa
       61 atgggttttt atttgatggt tgcttcaatg ctactcggac tgctcgcttt gaagataggt
      121 ttttctcagt tcaaggagaa caaagataaa ttcttatcta tcttaacaag tttagctggg
      181 ctagcccttg ttctcgtggc tgtctggtta ggatggccca aataaataga aaaacctcgg
      241 tcaaaccgag gtttttcaga tgaatttctt ggttgtggct aaaaaatatg ctacactatc
      301 aatatgaaga ttttaatccc tacagcaaaa gaaatgaata cagacttgcc gagtatcgag
      361 acaactcttt taagatcaga aagtcaggct gtacttgatg ccttggctct ctattctgcc
      421 agtcaattgg aaagtttcta caaggtatca gctgagaagg ctgcggaaga atgtcaaaat
      481 atccaagctt tgaaaaggca aactgctcaa cactacccag ccttgaagct ttttgatggg
      541 cttatgtacc gtcatatcaa gagagacaaa ctgaccgagg tagaacaagc ctatcttgaa
      601 aatcatgtct tcattacctc ggctttgtac ggagtcgttc cagccttgtc gcctatggca
      661 cctcaccgtt tggatttttt gatgaaatta aaagtcgctg gtaagacttt gaagagccat
      721 tggaaggcag cctatgatga agctgtgaag caggaagaag tgattttctc tctcttgtca
      781 tcagagtttg agactgtatt ttctaaggaa atcagagcaa agatggtgac cttcaaattc
      841 atggaggata gaggcggtca gctgaagatt cactcaacta tctccaagaa agcgcgcggg
      901 gcctttctaa cagctttaat agaaaatcaa gtacaaactg tgggggaagc acgtcgcttg
      961 aactttgctg gatttgttta ccgagaagat ttgtcacaac cacagggatt ggtttttgta
     1021 aaggaagtat agaaatcaga taagagaaaa gtcagcacga ttatgctgac ttttttgata
     1081 acttataaag tattggacaa atgaatctca tttgataagg tgttaagatg attaaccaaa
     1141 ttcatgattg acagatgatt tcggcaaata aacttcttgg taatccctga aatatagaaa
     1201 ttagacacga tgatatcgta atctgtctga tttaaaatct caggacttgt ttttaattca
     1261 tcccaaatat cgaaggtaaa gcggttatta cagtaatagg aaagcatatc aacaaaggta
     1321 agggatatag catggtcaaa gttactgata atcaaaacct tgataggtgg tcgattttct
     1381 aacaactgag tagagaggtt ttcagcatgg gttaagatgg tgtacatcaa gtgttccaac
     1441 tgttcaggat ggtcatgctg tccaattgcc tgacggtatt gagccagttc ttgacgcgca
     1501 cttcccataa agtctgggaa gtaaacttca aattttttaa tcgtcaatgc tttttgttca
     1561 aataagattg gggtagaaaa gatttcctgt ctttcaaaga aagcagtgtt gtgtaaatgc
     1621 caaatcagct catcgtggtt agtaaaagta atcttatatt gttttgatag gcgttctaat
     1681 atttgactaa gtaattggta agagtatctt gcttgactgt tatcttctaa agagttgaag
     1741 aattcttgag gatctaggaa aatatcattc tgaagaaaag aaataaaaat ttgagcgatt
     1801 gtatctggag tcatttcaag cccaaaatgt ttagagaaat aagcaagtgt ctcctggaaa
     1861 tttggaatgc tctttaaaag aggataatat tctttgtaaa agtggttggg gaggtcaatg
     1921 aagtgaccat ttttaaccct gtggatactg atagctatca tcagtttgta cattctataa
     1981 attgaaaatc gcattggata attcgtgatt ttatagaaga agtcagcaaa ctctgttaaa
     2041 tcttcctcag gaaggtcagg aaaaggccaa tctaggaaat aatagcgctc agaaaagtat
     2101 tgggcaaaaa agtaacgaat atcgatctca tttccacgta tctcggatgg agtaaaagat
     2161 aattcaattt gaaactgaga tgaaagaacc tttgtaatat ttctgcccag tcgatagagg
     2221 ttggcactac taaagtaaag attttctatt gttcgataaa taggtagccc ctcattgaag
     2281 aacatatact ctagtaattg aaaagattga gaattagcaa gaaaatattg ataaatatct
     2341 tctacactag tgttaacttc taaatctatc atgattccgt taacagaaga ttggatgtta
     2401 atagatggaa aggcaatacg taattcattt aaatcacttt ttaagattcg ttctgtacat
     2461 cctaattttt cggctagttc atgtaatttc atccaattac gattctgaat tagggtttcc
     2521 attaagcgta attgtctttg aacttttgtg gataaaaggt ttctcatacg ggtatttcct
     2581 ccataattca ttatatcttt ttttgtctaa gtagtataaa aaaattagag caagaaaatt
     2641 gtcgttagta atcggaaaaa ttagaataat ataagtggct tttaagtgca aaaacagaat
     2701 atattaattg ttcagaaaat attgattaat gcctcaaata gttgtacatt gtaaactata
     2761 aaatactatc taggagttta aaatgaaaca ttcacataaa aaatcatttg actggtatag
     2821 catgcaacaa cgttattcta ttcgtaagta ttactttggt gcagttagtg tcttgctcgg
     2881 taccgctttg gtattaggtg cagcagctag tgtccaaaca gtacaagcgg aagaaaacaa
     2941 acaagaaact accaatagta tttctgttgg taggggagaa gcagctacta aaccagcaga
     3001 ggtttctgcg tctaataaag agaaaaccta tgcagctcca actgtagcta atccagtaga
     3061 aacgactcca gttaaaactg gagaagttac taaaccagca gaaaaagttg aagaagcaaa
     3121 agacaaaaaa gaggaagtaa cgcatcaaga tgctattgac aagtcaaaat tgttaacggc
     3181 tctttcgcgt gctaaaaagt tagaaagcaa gttatataca gaagcaagtg ctgctaactt
     3241 gcaaacaagt atccaagctg gtcaaagctt gcttgggaaa gcagatgcat ctgaagctga
     3301 attatcagca gcagagtcat ctattcaatc atctattatt ggtctagaac ttcgttctaa
     3361 ctctaacaaa ggaactgtat cagaaacgcc tgtagcaaag aaagctaata tagttgaagc
     3421 gaaggaagag actaaaccag ctgtaacaac gactgaacgt tcagctgttg atagcgctat
     3481 tttgccaatt agcacagctg ctaaagtaga aacaacttca gctccagcat ctactaatga
     3541 aatcttgaaa ccaagtttga gcctttctga tgctcgccaa aatccagcta tccgtaagga
     3601 agatgttgat agagggtata gtggttttag aacagcagga agtggcttta gagcggctgg
     3661 aagtggtttt agagcagctg gaccagaaaa taaaccaatc ttaaatccaa ataatactat
     3721 tgcattttct gacatcagtc agggcttaca ttcatttcgt ggtataggac attcacgtgg
     3781 tggtcgtgaa atccattatg atgtaacgac agtacgtaga ggtaatagat tgaatttcac
     3841 aattaaatac tctggtccag gtgaatttgt taataataat tttatcttgg ataaagggga
     3901 tggatttgga aatccttcaa atgcaacgat cacaagttca aatccaagag taagggagca
     3961 atcaaaatct attagtcagg gtgccaacta cgtatctcac tcaggataca gtatgacctc
     4021 tgctacttca acaaatacgg aacaaactat cagatttagt ttgcctatca ttaatccaaa
     4081 tggtgatttg tctgttcgtt tgaaaccagt tacttttaac gtggaccaag gtggtggtgg
     4141 tgccgctact agcaatgacc catacagtaa ctccaactat tatcatagag caaatccact
     4201 cctcttggat gcaaaccctt atggtggtac taataataag actgtttcag aagacattga
     4261 cttccaaact gtctatcttc caactagtaa gttaccagaa ggtcaaacta gattagttcg
     4321 agaaggtgaa aaaggacaac gtcaaattac ctataaagta catcgatttg gtaacgaaac
     4381 acttttagga ttgccaatta gtaatagagt tactaaagaa gctaagccac gtattatgca
     4441 aattggtgtg gctaaagagt taatcgatac agtaaaacca cgtgttgatc aaaataaagt
     4501 tggtgataca aataacctca ctttctatct tgataacgat ggaaacggtg tttatactga
     4561 gggcgtagac gaacttgttc aaaaaattgc tattaaagat ggagccaagg gtgaaaaagg
     4621 agaccaaggt gaacgcggtc taactggagc taaaggtgaa aaaggagacc gaggcgaacg
     4681 cggtctaact ggagctcaag gagctaaagg tgaaaaagga gaccgaggtg aacgcggtct
     4741 aactggagct caaggagcta aaggtgaaaa aggagcccga ggtgaacgcg gtctaaccgg
     4801 agctcaagga gctaaaggtg aaaaaggaga ccgaggcgaa c
//
LOCUS       DAVLVF010000102         4644 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVLVF010000102 DAVLVF010000000
VERSION     DAVLVF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.102"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..643)
                     /locus_tag="VYN66_001803"
     CDS             complement(<1..643)
                     /locus_tag="VYN66_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1495785.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFN"
     gene            complement(931..1893)
                     /gene="nrdF"
                     /locus_tag="VYN66_001804"
     CDS             complement(931..1893)
                     /gene="nrdF"
                     /locus_tag="VYN66_001804"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV1495786.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(2081..4240)
                     /gene="nrdE"
                     /locus_tag="VYN66_001805"
     CDS             complement(2081..4240)
                     /gene="nrdE"
                     /locus_tag="VYN66_001805"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV1495787.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWTELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(4321..4539)
                     /gene="nrdH"
                     /locus_tag="VYN66_001806"
     CDS             complement(4321..4539)
                     /gene="nrdH"
                     /locus_tag="VYN66_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV1495788.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPEKLKQLA"
BASE COUNT         1350 a          857 c          952 g         1485 t
ORIGIN      
        1 tattgaactt actatgatct gcgagtaaat attttttatt agaattattt aaagcgatgc
       61 gttgagcctc tccctcttcc tcgctaaaag tagctagagc tccgttttga ataccattac
      121 agctaacgaa agctttagaa aattggagat tagagagatt ttgtagggtc aatgtgccaa
      181 caaaagcacc tgtaatatcg cgataatttc cacctatcaa aatcaaatct gttaattttc
      241 gttcgcttaa aatcagaaaa acaggtagac tgttggttac gacgcggata ttgtcaatag
      301 gcaactcacg cgcaaaaaac tctaatgttg ttcctggtcc aatgaaaata gtttctcttt
      361 cttctactag actgcctgca aaatgggcta tttcttgttt ttctgccgtt tggagggctt
      421 gtttttcaat atttgatcgc tcattagtca aaagggagtt ggttcgaagt ttttcagctc
      481 caccatgcac acgaatcagc aaatctttat cagctaattc ctgtaaatag cgccttgcag
      541 tcatatctga aacggctatt tcgtccataa tctgtttaac tgttatggtt cctttactat
      601 ttactatctc taaaattttg gttaattttt cttgtttgag catattatca cctcgtttcc
      661 tactactatc ttaccataaa caaactcatc attcaaatac aaaaacaaca aaatgaaaca
      721 aaaacaaaaa tatcgaagtt tgttttcaaa actttcgata tttttgttgg gttattattt
      781 tgttgtttct agtttacttt ttgatggtta agagtggtgg agaattatac tcaatgaaaa
      841 tcaaagagca aactaggaag ctagccgcag gctgtacttg agtacggcaa ggcgaagctg
      901 acgtggtttg aatttgattt tcgaagagta ttagtccaaa ccgtagttgt agtcatcatc
      961 ttgcatggct tcaacttcgc caagaaggta accatttccg acttgagaga agaagtcatg
     1021 gttggaagtt cctgttgaaa taccgttcat aacgattggg ttgacatctt cagctgaatc
     1081 tgggaaaagt ggatcttgtc ccatgttcat gagagcttta ttggcattgt agcgaaggaa
     1141 ggttttaacc tcttcagtcc aaccaacacc gtcataaaga ctctctgtgt agccttcttc
     1201 attttcataa agagtataga gtaggtcgta catccattct ttgagttttt cttgctcttc
     1261 ttcaggtaat tcattgaaac caagttggaa tttgtaacca atgtaggttc cgtgaacaga
     1321 ctcgtcacga ataatcaatt taatgatttc tgcaacgttg gcaagtttgt tgttaccgag
     1381 atagtagagg ggagtgaaga aaccagagta gaagaggaag gtttcgagga agacgctggc
     1441 aactttcttt tcaagtgggc tgccgtttag gtagatttcg ttgacaatct cagccttctt
     1501 ttgtaggtaa ggattggtat tggtccattc gaaaatttct tcaatctcag ccttagtatt
     1561 caaggtagaa aagattgatg agtaagattt agcgtggaca gattccataa attggatgtt
     1621 attgaaaaca gcttcctcat gtggtgtacg gatgtctgcg cgaagggctt gaaccccagt
     1681 ttcagattgc atagtgtcaa gaagggttaa accaccaaaa acttttccga ccaagtcttt
     1741 ctctttgtta gatagctttc tccagtcatc caagtcgttt gataagggaa tacgtgtatc
     1801 gagccaaaat tgctccgtca gtttttccca agttgatttg tcgatgacat cttcgatggc
     1861 attccagtta atggctttgt agtaagtttc catttaaaat ctctttctgt gtttagtatt
     1921 gcgaactcac aattatttct actttaccat aattctatag gagtatcgca caaaaagtcg
     1981 gaagcccgac ttttaaaatg ttacataaat tatgttatga catagtagat ttgattttat
     2041 cagtgctgct tagggaaaaa tagtgtttct atgctagaaa ctaaatcaca cagctttcac
     2101 attggttggc gccgacttct ccaccgtcat ctgtaaaggt acggacgtag tagatagact
     2161 tgattccctt gttaaaggca tagttacgaa ggatggacaa gtcacgtgtc gtttgtttat
     2221 tttccctctt ccattcgtaa aggccttttg gaatgtcact acgcatgaag agggtgagtg
     2281 aaagtccttg atccacgtgt tcagtcgcag cagcgtaaac atcgatgact ttacgcatat
     2341 ccatatcgta ggcagaagtg tagtaaggaa tggtttctgt agacaagcca gcagcagggt
     2401 agtagatttt accaattttc ttttcctgac gttcttcaat acgttgcgta atcgggtgga
     2461 tagaagcaga aacatcgttg atgtagctga tagaaccatt tggcgctaca gcaaggcgat
     2521 tttggtggta aagaccatcc tcttgaacct tgtcgcgaag ttcagtccaa tcagcaacac
     2581 cagggataaa aacatttttg aagagttctt taacacggtc tgatgttgga acaaattcac
     2641 cagttacata cttgtcaaag taacttccgt tagcatagtc tgatttttca aagttgtgga
     2701 aggtaatacc acgttcacgc gcaatattgt ttgactctac caaggtccag tagttcataa
     2761 gcataaagta gatgcttgta aattcaacag actcaggtga accatattca atgagttgtt
     2821 gggcaaggta gctgtgcagt cccatggcac cgagaccaaa ggtgtgggct tggctatttc
     2881 catggtcaat cgttggtaca gctacgatat gtgaactatc tgtaacgaaa gtaagggcac
     2941 gaaccatagc acggatagaa cgaccaaaat caggtgaagt catcatgtta accacgttgg
     3001 ttgaacccag gttacatgaa acatctgttc ccatttgaag gaattcttga gcatcgttga
     3061 tcaagcttgg ttcttgaact tgaagaatct cagaacacaa gttactcatg ataatctttc
     3121 catcgacagg atttgcacgg ttagccgtat ctatgttgac tacataagga tagccagact
     3181 cttgttgcaa tttagagatt tcagtttcca aatcccgcgc cttgattttt gtcttgcgaa
     3241 tatttggatt tgcgaccaat tcatcgtatt tttcagtaat gtcgatgtaa ttgaatggca
     3301 caccgtattc tttttctaca gagtaagggc tgaagaggta catttcttca tttttacgag
     3361 ccaattcgta gaatttatca ggtactacaa caccaagtga tagagtcttg acacgtactt
     3421 tttcatcagc gttttctttc ttagttgaaa ggaaagcgat gatatctggg tgaaagacgt
     3481 tgaggtagac aacaccggca ccttgacgtt gccccaattg gttggagtaa gagaagctgt
     3541 cttcaaaaag cttcataaca ggaacgacac ctgaagcagc tccttcatag cctttgatag
     3601 gtgcaccagc ttcacgaagg ttgctgaggg taattcccac accaccacca atacgtgaaa
     3661 gttgaagagc tgagttgata gaacgtccga tagagttcat atcatccgtc acttggatta
     3721 ggaaacaaga taccaactcc ccacgacgag cacgtccagc attcaagaag gaaggagtag
     3781 caggttggta gcgttggtgg atgatttcat tggcaatatc gattgcaaca gcttcattcc
     3841 catcagcgaa ataaagggcg ttgaagaaga cacggtcttc catattttca agatagtatt
     3901 caccgtcatt agtctttaag gcatattgat tgtaaaattt ataagctgcc atgaatgact
     3961 tgaattggaa gttttggtct ttgataaatt gagccaattc ttccaagaac tctggacggt
     4021 atttcttgat aaaggctgtt tcgatgtagt tgtgttcaat gaggtaattg attttgtctt
     4081 tgattgaatc aaaaaccata gtgtttggaa ctacattttc tttaaagaaa gcatccaagg
     4141 cttccttatc tttatgaagc atgatttgtc cattaacagg acggttaatt tcgttattaa
     4201 gacggaagta agtcacgtct tcaagatgtt ttaatcccat aaaatttccc ttatctaatt
     4261 acaaaagaaa ggcttctaag ttagccctaa aagcagtttc ttctggatga tgtactaaga
     4321 ttatgctaat tgtttcagtt tttctggttg gaaacctgaa aagacttcag ttggtgtttg
     4381 gataacagga gctgcgctaa aaccgagctc tttaacttga tcgacgtact caggttgctc
     4441 atcaagattg atttcacgat aagagacatt attactgtcc aagaaacgct tggtcatttt
     4501 acattggaca caattgtttt tagaataaac ggttaccatt gtgtaactcc tcttcaaaat
     4561 ttaatactat cttagtatat cagaaaataa aattttgtca atgatttaaa ctaaatatag
     4621 tggtgttctt ttgttgtttt tata
//
LOCUS       DAVLVF010000103         4573 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVLVF010000103 DAVLVF010000000
VERSION     DAVLVF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.103"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            96..329
                     /locus_tag="VYN66_001807"
     CDS             96..329
                     /locus_tag="VYN66_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV1495789.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            329..1663
                     /locus_tag="VYN66_001808"
     CDS             329..1663
                     /locus_tag="VYN66_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV1495790.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDENGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            1673..1927
                     /locus_tag="VYN66_001809"
     CDS             1673..1927
                     /locus_tag="VYN66_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV1495791.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            2023..2418
                     /locus_tag="VYN66_001810"
     CDS             2023..2418
                     /locus_tag="VYN66_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1495792.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLDLKGIGPATIKKLKENGIKFK"
     gene            2918..3280
                     /locus_tag="VYN66_001811"
     CDS             2918..3280
                     /locus_tag="VYN66_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495793.1"
                     /translation="MLAMEQAGFISHQEKLERLGVHYEISERTQTPILDACPLVLDCR
                     VDRIVEEDGICHIFAKILERLVAPELLDEKGHFKNQLFAPTYFMGDGYQRVYRYLDKR
                     VDMKGSFIKKARKKDGKN"
     gene            3267..3824
                     /locus_tag="VYN66_001812"
     CDS             3267..3824
                     /locus_tag="VYN66_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1495794.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSIYFNHRHEDDVMEIGY
                     TLHSDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSQRVAEKLGFTLEARI
                     RDRKDVQGNRCDSLIYGLLKSEWEE"
     gene            3826..>4573
                     /locus_tag="VYN66_001813"
     CDS             3826..>4573
                     /locus_tag="VYN66_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV1495795.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDTNFAYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYFNALTELYGNYNKLFLK"
BASE COUNT         1407 a          819 c         1055 g         1292 t
ORIGIN      
        1 acaacctcaa agcactgttt tgagcaacct gcggctagct tcctagtttg ctctttgatt
       61 ttcattgagt attagaacat acaatggagg tcgtcatgga caatatcatc gatgtgtcaa
      121 ttcctgttgc agaagtggtg gacaagcatc cagaagtctt ggaaattcta gtggagttgg
      181 gttttaaacc ccttgccaat cccttaatgc gcaatacagt tggtcgtaaa gtatcactta
      241 aacaaggttc taagctagca ggaactccta tggacaagat tgtacgcaca ctggaagcga
      301 atggctacga agtgattgga ttagactaat ggcagatgaa cggattcatg tcctacggga
      361 tattttatta gaattgcata atggcgcctc tcctgagtcg gttcaggagc gttttgatgc
      421 gacctttgcg ggtgtctcag ccattgagat ttcccttatg gagcacgagc tgatgaactc
      481 agactcaggt gtcacctttg aagatgttat ggaactctgt gatgtccatg ccaatctttt
      541 taaaaatgct atcaaaggtg tcgaagtttc agatactgag catccaggtc acccagttcg
      601 tgtcttcaaa gaagaaaatc tggctctccg tgcggccttg attcgcattc gtagattgtt
      661 agatacctat gagtctatgg aagacgagga aatgctggcg gagatgcgta agggtttggt
      721 gcgtcagatg ggacttgtgg gtcaatttga catccattac caacgtaagg aagaactctt
      781 ctttcctatc atggagcgct atggacacga ttcacctccc aaagttatgt ggggagtgga
      841 tgatcagatt agggaactct ttcaaacagc tctaacgaca gccaagtcac taccagaagt
      901 gtcaattagc agtgtaaagg aagcttttga agcttttgcg acagagtttg aaagtatgat
      961 tttcaaggaa gagtccatcc tcctcatgat tctccttgag tcttttactc aggatgactg
     1021 gcttcagatt gcggaggaga gcgatgccta tggctatgcc atcatccgtc cgtcagagaa
     1081 atgggtgcca gaacgacaga gctttattga ggaaaagatt gcagaggagc ctgtacagct
     1141 agatacggca gaaggtcaag ttcaacaagt catagatacg ccagaaggcc attttaccat
     1201 tacctttacc cctaaggaaa aggaagctgt gctggaccgc catagtcaac aggcttttgg
     1261 taatggctat ctttcagtcg agcaggccaa tctcatcctc aatcatctcc ctatggagat
     1321 tacctttgtc aataaagaag atattttcca gtattacaat gacaatacgc cagctgatga
     1381 gatgattttc aaacggacgc cgtcccaagt cgggcgcaat gtcgaactct gtcatccgcc
     1441 taagtatttg gacaaggtca aggctatcat gaagggactt cgtgagggga ccaaggacaa
     1501 gtatgaaatg tggttcaagt ctgagtcgcg aggcaagttt gtccacatca cctatgctgc
     1561 agtacacgat gaaaacggag aattccaagg tgtactggaa tacgttcagg atatccagcc
     1621 ctaccgtgag attgatacgg actatttccg tggattagaa taaggagaaa aaatgagtta
     1681 cgaacaagaa tttatgaagg aatttgaagc ttgggtcaat acccaaatca tgattaacga
     1741 catggcgcac aaggaaagcc aaaaagttta cgaagaagac caggacgagc gtgccaaaga
     1801 tgccatgatt cgctacgaga gtcgcttgga tgcttatcag ttcttgcttg gtaagtttga
     1861 aaacttcaaa gtaggcaagg gattccatga tttgccagaa ggcttgtttg gtgagcgaaa
     1921 ttattaaacg agaaagattc ttgatttttc actaaaatct tgatagaatg tttatgttaa
     1981 atccttgtca gagcagggat tttttattga aaggatttta tcatgtcaaa gaaactcaat
     2041 cgtaaaaaac aattacgaaa tggcctccgt cgcgcaggtg ccttttcaag tactgtgact
     2101 aaggttgtag atgagacaaa aaaagtcgtg aagcgtgcag aacagtcagc aagcgcagct
     2161 ggtaaggctg tttctaaaaa agttgaacaa gcagtagaag ctaccaaaga gcaagctcaa
     2221 aaagtagcta attctgtaga agattttgca gcaaatttgg gtggacttcc acttgatcgt
     2281 gccaagactt tctatgatga aggaatcaag tctgcttcag atttcaaaaa ctggactgaa
     2341 aaagaactcc ttgacttgaa aggaatcggc ccagctacca tcaagaaatt gaaagaaaat
     2401 ggcatcaagt tcaagtaatt tttcttgagc cttgcatttc cgaaaaaatc ttgctacaat
     2461 agagccatta gaggtgtttt gaatcccaca ttttacagaa agtggcggcg ctgagaagtc
     2521 cacaaatgtg tcaaaactgg ttgctaatgg atgaaaaatt gaaataaaag tgtctttttg
     2581 ctttaaagac gagagttgcg ggcaactgcc cgaaattggg tggtaccgcg gataaacaca
     2641 ttcgtccctg tcatgtagat ggcagggctt ttcttttacg tcttagtcaa agggggttgt
     2701 tatgaagcaa tcttttaaca caagtaaact atattatggt tttcctgttt ttattttagg
     2761 atatcaagat tagaattttg gctataacat cacgacctgt agttcctctt atagtctagg
     2821 agattgggtt gtgattggag tcgttgcgag agagaatgcc gcagagtaga tcaaacagta
     2881 tcaaaaattt actgtgaata tttctgatga aacttctatg cttgcgatgg agcaggctgg
     2941 ttttatcagt catcaggaga aattggaacg tttgggagtg cattatgaaa tttctgaacg
     3001 aactcagact cctattttag acgcctgtcc acttgtttta gattgtcggg tagataggat
     3061 tgttgaggaa gacggtattt gccacatctt tgccaagatt cttgagcgac ttgttgcccc
     3121 agaactcctg gatgaaaagg gacattttaa aaatcaactg tttgccccaa cctatttcat
     3181 gggagatgga tatcagcgcg tttatcgcta tctggacaag cgtgtagata tgaagggcag
     3241 tttcatcaaa aaagcgagga agaaggatgg caagaactga gctgccagat aaaatcgaaa
     3301 cagaacgtct cgttttacga gtccgtactg tggcggatgc tgaggatatc tttgactatg
     3361 ctagtttgcc agaggtcgcc tatccagcag gttttcctcc agtcaagacc ttggaagatg
     3421 agatttatta tctggagcac attcttccgg agcgtaatca aaaggaaaat ctcccagcag
     3481 gctacgggat tgtcgtcaaa ggaacggata agatcgttgg ctctatctat ttcaaccatc
     3541 gccatgaaga tgatgtgatg gaaatcggct ataccttaca ctcagactat tgggggcgag
     3601 gttatgtgcc agaagctgcg cgtgccttga ttgacttagc ctttaaagat ttgggtcttc
     3661 acaagattga actaacttgc tttggatata accttcaaag tcaacgagtc gcggaaaagc
     3721 ttggctttac cctcgaagct cgcataagag accgaaagga tgttcaagga aaccgctgtg
     3781 acagtctgat atatggcttg ctgaagagcg agtgggagga ataagatgag cgatgtaaaa
     3841 gaggaagtaa gctctctatc tgagaagcag ttacgccaaa ttgatgttga gtatgctgaa
     3901 ttaaatgata gtgatattat cgagcgatta gcttatttag aaattaacaa caatgaaaaa
     3961 cgtattgtca tctctgatat tgaaccaacc aaggaaataa tgtcagtctc ggaccaaatt
     4021 tttgagattc aaaagaattt tcaaaaaatc aagaatatgt tcgaattgtt tatttcagat
     4081 gtctcggatt ttttaagtat taaaaataaa cttgagtcaa aggaattaga aattgaagag
     4141 gcagatgtaa atcgtttcat gattcattta ttatccagtg gaaaattgtt tgtcgatttt
     4201 aacgaaaacc aaattaaaca aaaatattcc aaagatagtg aggagtttga ttgtatacat
     4261 ggttttgcaa gctatcagta tgataccaat tttgcttatc gattttgcca ttctttgaga
     4321 aattattcgc aacatactga cttaccaata aatgaggtaa aagctgttag cccagatgat
     4381 gagactgtta taatagattt ctatattgat ttagattatc ttttaaattc caactttaaa
     4441 tggaaaaaat tgaaagggga gttgataaaa ctaaaccaag aaacatctaa gattgacgct
     4501 atagctctag tgaaagaata ctttaatgct cttacagagc tctatggtaa ttataataag
     4561 ttgtttttaa agt
//
LOCUS       DAVLVF010000104         4546 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVLVF010000104 DAVLVF010000000
VERSION     DAVLVF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.104"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            22..846
                     /locus_tag="VYN66_001814"
     CDS             22..846
                     /locus_tag="VYN66_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1495796.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIKHKTGWFDGMMTTGCSMGAYHALNFFLQHP
                     DVFTKVIALSGVYDARFFVGDYYNDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCT
                     GLGAWEQDGLPSFYKLKEAFDQKQIPAWFAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            859..2025
                     /locus_tag="VYN66_001815"
     CDS             859..2025
                     /locus_tag="VYN66_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1495797.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            2533..4026
                     /locus_tag="VYN66_001816"
     CDS             2533..4026
                     /locus_tag="VYN66_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1495798.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            4039..>4546
                     /locus_tag="VYN66_001817"
     CDS             4039..>4546
                     /locus_tag="VYN66_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1495799.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKGLITKRK
                     LQLDELNLL"
BASE COUNT         1474 a          790 c          948 g         1334 t
ORIGIN      
        1 aatatagaga gttttctgac aatgaatcaa tcctactttt atctaaaaat gaaagaacac
       61 aaactcaagg ttccttatac aggtaaggag cgccgtgtac gtattcttct tcctaaagat
      121 tatgagaaag atacagaccg ttcctatcct gttgtatact ttcatgacgg gcaaaatgtt
      181 tttaatagca aagagtcttt cattggacat tcatggaaga ttatcccagc tatcaaacga
      241 aatccggata tcagtcgcat gattgtcgtt gctattgaca atgatggtat ggggcggatg
      301 aatgagtatg cggcttggaa gttccaagaa tctcctatcc cagagcagca gtttggtggt
      361 aagggtgtgg agtatgctga gtttgtcatg gaggtggtca agccttttat caagcacaaa
      421 acaggttggt ttgatggcat gatgacgaca ggttgctcta tgggagccta tcatgcactc
      481 aatttcttcc tccagcatcc agatgtcttt accaaagtga ttgctctcag tggtgtttac
      541 gacgcacgtt tctttgtcgg cgattactac aacgatgatg ctatttacca aaactcgcca
      601 gtagattata tttggaacca aaacgatggc tggtttattg accgttaccg tcaggcagag
      661 attgtgctgt gtacggggct tggagcctgg gaacaagatg gtttaccatc cttttacaag
      721 ctcaaagaag cctttgacca gaaacaaatt ccagcctggt ttgctgaatg gggacatgat
      781 gtcgcccacg actgggaatg gtggcgtaaa caaatgcctt atttcctcgg taatctctat
      841 ttataaaagg agttacctat gaattacctt gttatttctc cctactatcc acaaaacttt
      901 caacagttta ccatcgaact agctaataaa ggcatcacag tcttgggaat tggtcaagag
      961 tcttacgagc aattggatga gcccttgcgc aatagcttga ccgagtattt tcgtgttgat
     1021 aatcttgaga acatagatga agtcaaacgt gcagttgctt ttctctttta taaacatggt
     1081 ccaattgacc gcatcgagtc tcacaatgaa tactggcttg agctagacgc aacactcaga
     1141 gaacaattca atgtttttgg tgccaaacca gaggatctca aaaagacgaa atataagtct
     1201 gaaatgaaga aacttttcaa aaaagcaggt gttcctgtgg tacctggagc tgttatcaag
     1261 acggaagcag atgttgatca agcagtgaaa gaaatcggtc ttccaatgat tgccaaacct
     1321 gataatggag tgggagcagc cgcaaccttt aaacttgaga cagaagacga tatcaatcac
     1381 ttcaagcaag aatgggacca ttcaaccctt tatttctttg aaaaatttgt cacttccagc
     1441 gaaatctgta cctttgacgg gctcgtggac aaggatggaa agattgtctt ctcaacaacc
     1501 tttgactacg cctatacacc gcttgacctc atgatttata agatggacaa ttcttattat
     1561 gtgctcaagg atatggatcc taaactgcgc aagtatgggg aagcaattgt caaagaattt
     1621 ggtatgaaag aacggttttt ccatattgag ttcttccgtg agggggacga ttatattacc
     1681 atcgagtaca ataaccgccc tgcaggtggt tttaccattg atgtttataa ctttgctcat
     1741 tccttggacc tttatcgtgg ctatgcagct attgtcgcag gagaggagtt cccggcgtca
     1801 gactttgaaa ctcagtattg tttggctact tctcgccgtg caaatgctca ctatgtttat
     1861 tcagaagagg atttgcttgc caaatatagc cagcagttca aggttaaaaa agtcatgcca
     1921 gctgccttcg cggaacttca aggagattac ctgtatatgc tgacaactcc gagtcgacaa
     1981 gaaatggatc agatgattgc tgatttcgga caacgtcaag aataagaact atcggattaa
     2041 ggaaattcac tcccttaatc cttttgtttt gtctggtaaa aaataagagc atcctaacaa
     2101 ggtagctatc ataaaacttg ttcgataact atttgaagca ggattaggtg gctagaaatt
     2161 gaattttaac atttcaattt agtcatagta ttgtgttttc gtatccttaa atcagctaaa
     2221 aggatccatg acgacaccta tacgatatag ttttcaggat accaaaccag tctattaata
     2281 ctcaatgaaa atcaaagagc aaactaggaa gctagccgca ggttgctcaa aacactgttt
     2341 tgaggttgtg gatagaactg acagagtcag tatcatatac ctacggcaag gtgaagctga
     2401 cgtggtttga agagattttc gaagagtata aaatattcag gtgacgcata gatatagtta
     2461 attgaagctt tgtttgaaat ctgataaaat aatgatatta ctaagtttta aaaactaaag
     2521 aaaagggaag atatgattac aggcgaatta aaaaataaaa tcgatcagct gtgggaaatt
     2581 ctttggacag aaggaaacgc aaatccttta acaaatattg aacagttgac ttatctctta
     2641 tttatgaaag atttggatag tgtcgagctt ggacgtgaaa gtgatgctga atttctaggg
     2701 attccttatg agggagtttt tccaaaagat aaacctgaat accgttggtc aacttttaaa
     2761 aatataggag atgctcagga agtttatcgt ttaatgactc aggagatttt tccgtttatt
     2821 aaaaatctca agggggatac agatgataca gccttttcac gatatatgcg agaagctatt
     2881 tttcaaataa ataaacctgc tacgcttcaa aaggcaattt ctatcttaga tgtttttcca
     2941 actaggggat tagatgtaga ttttgataat gacaaacaaa gtattactga tatcggagat
     3001 atctatgaat atctgttatc aaaattgtcg accgcaggta aaaatggaca gttccgtaca
     3061 cctcgtcaca tcatcgatat gatggttgag ttgatgcaac cgactatcaa agatatcatc
     3121 tcagatcccg ctatgggttc tgctggcttc ttagtatctg ctagccgtta cttaaagcgt
     3181 aagaaagatg aatgggaaac caatacagat aatatcaatc attttcataa tcagatgttt
     3241 catggaaatg atacggatac gactatgttg agacttgggg cgatgaacat gatgctacat
     3301 ggagtagaaa atccacaaat cagttacctt gactcgctgt ctcaagataa tgaagaagcc
     3361 gataaatata ctttggtttt agcaaatcct ccttttaagg gctcacttga ctacaattca
     3421 acctctaatg accttcttgc aaccgtaaaa accaaaaaaa cagaattact ctttctttct
     3481 cttttcttgc gaactttaaa accaggtgga cgagcagcag ttatcgtacc tgatggtgtc
     3541 ctttttggtt cgtctaaagc tcataaagga attcgtcagg aaattgtaga gaatcataag
     3601 cttgatgctg taatctcaat gcctagtggt gtgttcaagc cttatgctgg agtttcaact
     3661 gccattctca tctttacaaa aactggtaat ggtggtactg acaaagtctg gttttacgat
     3721 atgaaagcgg atggtttaag tttggatgat aagcgacaac cgattagcga caatgatatt
     3781 ccagatatta tcgaacgctt tcatcatctt gaaaaagaag cagaacgtca gagaacggat
     3841 caatctttct ttgttccagt tgctgagata aaggaaaatg attatgattt gtctatcaat
     3901 aaatataaag agattgagta tgaaaaagtt gagtatgaac caacagaggt catcttaaag
     3961 aaaatcaatg atttagaaaa agaaattcaa gctggcttgg ctgaattaga aaaattactc
     4021 aagtagggag gtggctgtat gaaaaaagtg aagttggggg aagtcttatc tctaaaaaaa
     4081 ggcaagaaag ccactgtact tgctgaacaa acaactctaa gccaacgtta tattcaaata
     4141 gatgatttaa gaaataataa taatttaaaa ttcactgaaa gtttaaatat gactgaagca
     4201 ctcccagatg atattctgat agcatgggat ggagctaatg caggaacagt tggttatgga
     4261 ttatcgggag ctgttggtag tacaattacg gtcttaaaaa agaatgagcg atacaaagaa
     4321 aaaattatat cagattactt gggagtcttt ttggaaagta aatcgcagta tttacgagaa
     4381 cattcaacag gtgcaacaat tcctcattta aacaagaata tattacttga tttacaatta
     4441 gaattgctag gtatcgaaga acaagagaac attatctgta ttcttaatac gattaaaggg
     4501 cttattacta aaagaaaatt gcagttagat gaactaaact tgctcg
//
LOCUS       DAVLVF010000105         4530 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVLVF010000105 DAVLVF010000000
VERSION     DAVLVF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.105"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(131..202)
                     /locus_tag="VYN66_001818"
     tRNA            complement(131..202)
                     /locus_tag="VYN66_001818"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(167..169),aa:Arg,seq:cct)
     gene            complement(244..591)
                     /gene="rplS"
                     /locus_tag="VYN66_001819"
     CDS             complement(244..591)
                     /gene="rplS"
                     /locus_tag="VYN66_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV1495800.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(710..1039)
                     /gene="crcB"
                     /locus_tag="VYN66_001820"
     CDS             complement(710..1039)
                     /gene="crcB"
                     /locus_tag="VYN66_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1495801.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1033..1407)
                     /gene="crcB"
                     /locus_tag="VYN66_001821"
     CDS             complement(1033..1407)
                     /gene="crcB"
                     /locus_tag="VYN66_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1495802.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(1404..1670)
                     /locus_tag="VYN66_001822"
     CDS             complement(1404..1670)
                     /locus_tag="VYN66_001822"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV1495803.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(1786..2229)
                     /locus_tag="VYN66_001823"
     CDS             complement(1786..2229)
                     /locus_tag="VYN66_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV1495804.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            2333..3268
                     /locus_tag="VYN66_001824"
     CDS             2333..3268
                     /locus_tag="VYN66_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV1495805.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3322..3606
                     /locus_tag="VYN66_001825"
     CDS             3322..3606
                     /locus_tag="VYN66_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV1495806.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3663..3881)
                     /locus_tag="VYN66_001826"
     CDS             complement(3663..3881)
                     /locus_tag="VYN66_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495807.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     IAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(3878..4452)
                     /locus_tag="VYN66_001827"
                     /pseudo
     CDS             complement(3878..4452)
                     /locus_tag="VYN66_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1403 a          997 c          807 g         1323 t
ORIGIN      
        1 ctcaatttta tctgatgatt gcccaaattt ttcaataata aaacgcataa tattatggag
       61 acaatcccct atattatgcg ttcttttaat atcaaagact ttttgacaaa cttcttcgat
      121 atctaattac tgccccctgc aggaatcgaa cctgcaacta ctccttagga gggagttgtt
      181 atatccattg aactaaggga gctagataaa aactctgctg aaaagcagag ttttttagtc
      241 gaattaacga cggatttctt tgatacgagc tgccttacct tgaagagcac gcaagtagta
      301 caatttcgca cgacgtactt taccgtaacg aacaacttcg attttttcaa cacgtggagt
      361 gtggattggg aagatacgct caacacctac accgttagag attttacgaa ctgtgtagtt
      421 ttctgagatt ccagcacctt tacgtgcgat aacaacacct tcaaaaatct ggatacgttc
      481 acggttacct tcgacaactt tcgcatgtac acgaacagtg tcaccaggac ggaatgatgg
      541 gatatctgta cgaagttgac cttcagtcaa gctttggatt aatggattca ttttattctc
      601 ctatctttgt caatcttgag gaatcttcct cagcggataa actgtatttt tgtgcgtcca
      661 ttacacacaa gatatagttt accaaatttc taaaaaaaag taaagaaatt tatagcaaaa
      721 ttcctaaaaa aatcgcaacc aaaccaccta tgtaagtcaa agtcaggtaa gaataaaaga
      781 ccttcttatc acttagcagt ctttgaagtt cgtcattcaa ggtcgaaaaa gttgttaaac
      841 cgccacaaaa tcctgttgct agaatagcat atacttcctt agactctaca tgattgtaga
      901 atactccaat taaaaaacaa cctaaaagat tagctatgag cgttccgagt ggcaatttag
      961 aagcttgatt atagcgagaa aagaaatacc gtacgagtgc tcccaagcca caggcgattg
     1021 caagatagac gattaccatt tcttcctccc caaatagtaa gctaaaagca ggcctccacc
     1081 gatactcaaa agcaaataca gtatcaaact aagataacgc cctgtatcaa gcagcttcac
     1141 agtatcaagc attaggctgg aaaaagttgt taaacctccg caaaaacccg tccccagtgc
     1201 taaaatcaag cccttactag tccccttata gaccagatag cccttgacaa gataaatcaa
     1261 gcagaaaatt cccagatagt tgacaaagag agtgccccaa gggaaatctg gactggctgg
     1321 taaccaagtg gaaactaggt agcggacaag tccgcccaac atagcagcta gaaaaatccc
     1381 tagcggataa aattgttctt ttttcatttg atattttgat cctgataatc acgcgaacgt
     1441 ttgagtatgt ccgaaaaagt cgcgacaata gtctcctgat agcgcttgtc ctctacacga
     1501 cttctaacct tttcaaaaat aactgcttct ctcttagtat ctaaaatcgg tttccctgaa
     1561 gccttcttat aagcgacaac tccttcaact aagtgcattc gttcttctaa cagtttgaca
     1621 atttggtcgt cgatttgatc gatttcttga cgaataatat ctaaatccat acagtctcct
     1681 cctttatttg aattattgta tcaaaaagcc atcaattagg ctagtaaaat cccaactctc
     1741 gataagaaaa atgcactgat tgattacatc agcgcacttt tatgcttatc ctactttagc
     1801 agccaattct tctgcgaatt gttccaagcg ttcaatatct tcttcctcgg cagaaagatc
     1861 aactttaaca cactctgaac ctttttctgc tcctgttgac acaaagacac ggtcaaagtc
     1921 atcaacagcc ttacaaaatt catcgtagaa ggtatctcct gagccgacca ctccgtagat
     1981 tttgccattc aagttgagat ctgctaggtc ttcgtagaag tccatcatct catctggcaa
     2041 ttcaccatca ccataagtat aggtcgcaac gatagcgatg tctgcttcca agaagtctga
     2101 agcgtcaaca gttgtacatt catcaacatc aacatccaag cccaagtcac gtaatttgtc
     2161 tgctacaata tctgcaattt cttcggtatt accggtcata ctggcaaata caatttttgc
     2221 taatgccata tcgtcctcct caatttatct ttctccatta tatcacatct ttttcatttt
     2281 aaaaataaaa attcttgtgc tacaatgaaa tgagaaagaa ttgaggttat ttatggagat
     2341 ttgccaacaa attttagaaa aaattaaaga atacgacacc attatcattc atcgtcatat
     2401 gaaaccagac cctgatgcct tgggaagtca ggtgggattg aaagccttgc tagaacatca
     2461 tttcccagaa aaaaccatca aagccgtcgg ttttgatgaa ccaactctta cttggatggc
     2521 tgagatggat cttgttgaag atagagccta ccaaggcgca cttgtcatcg tctgtgatac
     2581 agctaatact gctcgtatcg atgataagcg ctatagtcaa ggtgattttc tcattaagat
     2641 tgaccaccat ccaaatgatg atgtatacgg tgacctgtct tgggtcgata ctagttcaag
     2701 tagcgctagc gagatgatta ccctatttgc ccaaacaacc caactagcct tggcagatcg
     2761 cgatgctgag ttgctctttg caggaattgt cggtgataca ggtcgcttcc tctacccttc
     2821 taccactgca cggactcttc gcctggctgc ttatttgaga gaacataact ttgactttgc
     2881 ggctctcact cgcaaaatgg acactatgag ctacaaaatt gctaaactgc aaggctacat
     2941 ctacgaccat ctggaagtgg atgaaaatgg tgctgctcgc gttatcctga gtcagaaaat
     3001 cttgaaacaa tacaatataa ccgatgctga aactgcggcc attgtaggtg cacctggacg
     3061 cattgacaga gtgagtctct ggggaatttt tgtcgaacag gctgatggcc actaccgagt
     3121 tcgcttacgc agtaaagtcc atcctatcaa tgaaattgcc aaggagcatg atggtggagg
     3181 ccaccctcta gcaagtggtg ctaattccta tagcctagaa gaaaacgaaa tcatctacca
     3241 aaagttaaaa aacttgctta aaaactgata aaatacttgc caaacttttc agaatctgat
     3301 agactagtat agtaacaatc tatggctcgc aaagagacca tggcagaaag gaaatattgc
     3361 aaaatgaaaa aagatatcca tccagaatat cgcccagttg tcttcatgga cacaactact
     3421 ggttaccaat tccttagcgg ttcaacaaaa cgctctaacg aaacagttga gttcgaaggc
     3481 gaaacttacc cattgatccg tgtggaaatt tcatcagact cacacccatt ctacactgga
     3541 cgtcaaaagt tcactcaagc agatggacgc gtggatcgtt tcaacaaaaa atacggtctc
     3601 aaataatgat aagagaacag ttttggctgt tcttttttgt ttcttgaaat caactgctgt
     3661 tttcatgttc cagactcatc tgtaggttcg atttccatgc tactaggcag gaaggaaata
     3721 gctgtttcaa cacgtccata atgagctata ctattgtcac gaaccacact ttcattgatg
     3781 gtccaagtgg aattcatttt cttaaaagct tctcggactt tttccaaatc tttggaggca
     3841 atggcctgct ctaaggtttc aaaacgagga cttatactca tctgctttca aaaagcattc
     3901 tagtccatct ccgattaccg atggacttta tcacctcctt ctccagtcct tgtatgacat
     3961 cttgaagttg attcatgaca tcttccaaag ttcgaaaggc tttattctta aatccacgtt
     4021 tacgaatctc tttccacact tgttcaatgg gttcatctct ggtgtgtatg gaggaataaa
     4081 ggtaaaatca atattagtcg gaatatttaa ggtacttgat ttatgccata tagcattgtc
     4141 cataacgagt aaaaggataa gcttgtgaaa gctcttctaa aaaggcgttc atccacactc
     4201 ctttttataa acctgaaata aggcatcaat tgtaacaaat tctcctgcct ctgtagcctt
     4261 caaatgacgg gcaagaaagg ctttctcttc ctcaactgtc atatatgcat ggttacgacc
     4321 accacgtgtt tcttgaagga gagagtcgag tccgaactcc tcatattttt ttacgtttcg
     4381 ccaaatcgtt gtttgattac agtctaaaag ctctataatc tctttataag atttgcccat
     4441 cagacgaaat atagtagatt gaaactagaa tagtacacct ctacttctaa aacattgtta
     4501 gaaatcgatt tgtcctgttc ttgtttcatt
//
LOCUS       DAVLVF010000106         4493 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVLVF010000106 DAVLVF010000000
VERSION     DAVLVF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.106"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(275..826)
                     /locus_tag="VYN66_001828"
     CDS             complement(275..826)
                     /locus_tag="VYN66_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1495808.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(1176..2000)
                     /gene="nadE"
                     /locus_tag="VYN66_001829"
     CDS             complement(1176..2000)
                     /gene="nadE"
                     /locus_tag="VYN66_001829"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV1495809.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(1997..3457)
                     /locus_tag="VYN66_001830"
     CDS             complement(1997..3457)
                     /locus_tag="VYN66_001830"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV1495810.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3573..4061)
                     /locus_tag="VYN66_001831"
     CDS             complement(3573..4061)
                     /locus_tag="VYN66_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495811.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(4051..4260)
                     /locus_tag="VYN66_001832"
     CDS             complement(4051..4260)
                     /locus_tag="VYN66_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1495812.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
BASE COUNT         1283 a         1037 c          833 g         1340 t
ORIGIN      
        1 gatagaggat tctattttaa atttaacaac agctaggatt acactataaa aagtacatta
       61 ccacctgttc ggctggagaa tctagtcgat taggtccctc ttagttacaa gcttaaagcg
      121 attttttaac taatactaaa ccagattaaa gaaggaacta gaacatctgt cttgtactaa
      181 tcaatatgct attttaaagt ttcaaataaa cttggaatgc taattattat atttacaata
      241 aatagttgac ttcgatagaa acagaaaata gaattcaaca ttcttcccta cttataccat
      301 aataaataag atccgcaaga tagccttttc tctctacacc attaacaatg gtttgtagat
      361 aggacattcc agccttttcc atgacacgac ctgaagctgg gttgagactg gcataacgtg
      421 ctctgacctt ttgaaaacct gcttgagtaa aacaaaagtc caagatagct ttcaaggcct
      481 ctgtcatcat accatgtccc cagtaagcct tgcctagcac atagccgatt tcacagctta
      541 aatcagcctc gtctatctta acaatgctga tatctcctat cacttgctct gggttttctt
      601 ttagacaaat ggcccattta taatagttga gattagtata ggaagcaacc caattgcgaa
      661 tcgagtttcg agtgatttcg atatcaggat ggggatccca ggtaacatag gtcagattct
      721 cagcggatga agcccaattt tgaaacatag cttctgcatc actctccaca aatcttctta
      781 aaatcaaacg ttctgtctgt aatatttgcg taccaataga tttcatgatg ctacctcact
      841 tccttatttc tatgctcgta gtaaaaaggc tcaactcagt aacagctaga aaacgagttt
      901 gtcttctaat ttcttgacgc aatcgctgcc tggtttgaaa tgcgatctag caagcttttt
      961 caaacctagt catcgttgca ggggtaagac gatgaaatcg aactcttcga gttaccgaat
     1021 ctgcctcatt ttcttatttc taaactcggg gttcaagatc ccccagacct tctctcactt
     1081 tcttatttta agatgagagg ctgaaaacct ccagtggagg tttttcttgc tttttaattt
     1141 cagggcaaga agctaaaaag gtcccccgga cctttttact cccaaaagtc atcaaataca
     1201 gtgatgggta agtggcgttt gtgttggcct ttgtgccacc agttttcaat ggtcgcttga
     1261 gcttctgggc tgattgtttt gccttctagg tagtcgtcaa tctctgcgta ggtgactcca
     1321 agtgcgactt cgtcagctag gcctggttta tcttcttcta ggtctgccgt tgggattttt
     1381 tcataaaggg ctggctctgc gccaagtttc tgcaagagct gttttccttg gcgtttattg
     1441 aggcggtaaa gagggagaat atccgcaccg ccgtcaccaa acttggtaaa gaaacctgtg
     1501 atattttccg cggcgtggtc tgttccaatg accgctccgc tatgggaacc agcaagggca
     1561 tactgagcaa tcatacggca acgtgccttg atattcccct tgttgaagtc tgaaacagga
     1621 ctacctgtcg cttcaactgc agctgtcatg gcatcagctg attccttgat attcacaacc
     1681 aagctgacat ctggctggat gaaggctaga gctttttgag catctgcttc atcagcttgc
     1741 actccgtatg gcaggcggac agcgataaat ttgtagctat cgtctcccgt ttcagctcgc
     1801 agttcttcca tagctaattg cgccaaacgc cctgccaagg ttgagtcttg tcccccagaa
     1861 atccctagta caaaggtttt taggaaggga tgttttttca gatatctttt taagaaatca
     1921 atagaacgac ggatttcttc ctgggcatca atcactggtt tgacacccag ctcttggata
     1981 atcgtttctt gcaaactcat tcttcttctc cttcaccaag ggcttccttg cgcatcttat
     2041 caatcaagtc catcttatct tgccatacat cacgcgccaa atccactgga tagtgctgcg
     2101 gattgagcac acgcttatac tcatcccaca acttgtcaaa ttctttacgg gcataatcct
     2161 gaatgtcagt caaactaggc aagttgtaaa ctaatattcc ttctttgaag atatccacca
     2221 agagaggaac ggcatcaaaa ttacgaaccg tcttcttgat gtatgtatag gtcggatgga
     2281 acatcttgat ttctgtcatg tcgctaatat ccacaccatc ataagtgata tagtcgcctt
     2341 ctgacttgcc tttttcacga ctggtaatgc gccacacctg cttcttacct ggcgtagaaa
     2401 ctttttcagc attattagac agcttaatcg tattgcgcat ctgaccagtt tcatcttcga
     2461 ttgcaacaat cttgtaaact gcaccaagag ctggctggtc ataggctgta atcagcttgg
     2521 tacccacgcc ccagacatca atcttggcct tttgcatctt gagattgagg atagtatttt
     2581 cgtccaaatc attagaagca taaatcttag cctctgtaaa tccagcctcg tccagttgct
     2641 gacggacttt cttagaaatg taggcaatat ccccagagtc aatccgcaca cccataaagt
     2701 taatctgatc acccagctca cgcgccacct gaatggcagc tggtacaccg atgcgaaggg
     2761 tgtcataggt atccacaaga aagacacaat ttttgtgggt cgcagcgtaa gccttgaaag
     2821 cttcatagtc attgccataa acctgtacca aggcatgggc atgggttccc aaaacaggaa
     2881 tgtcaaagag cttacccgca cgcacgttgc tagttccatt ggcgccacca atcacagctg
     2941 cgcgtgttcc ccagatggcc gcatccattt cttgagcccg acgtgtccca aactccatca
     3001 agggttcatc ttcgataacc gaacgaatac gagctgcctt cgtcgccacc aaggtctggt
     3061 agttgacgat gttcaaaaga gccgtttcga ccaactgaca ttgggctaga ggtccttcca
     3121 cctgcacaat cggttcatta gcaaaaacca aatccccttc ttgggcagaa cgaacggtca
     3181 actccaactt gaaattgcga aggtaatcca agaacgcccc atgataacca agcgactcca
     3241 aataggctat atcactatct gaaaaacgca agtcttcaag atagttcaca attctttcca
     3301 aacctgcaaa aaccgcatag ccgttcttaa aaggctgttg gcggaaatac acctcaaaga
     3361 ccgccttctt attgtgaatc ccttggtcaa agtaaacctg catcatgttg atctggtaca
     3421 agtccgtgtg caatgtcaaa ctatcatctg gatacatact tttcctactt ccttagctag
     3481 aaacccatga aaattttcaa gaactttcat gtattccaat aaattagtac tattatatca
     3541 cattttagct ggattgagaa aagagtaaca agctattctc cactctccaa ttcatccata
     3601 tcttgttcaa attttttctg agcccattcg ccatagctct taagaccaag attgccaata
     3661 aagatccacg gaaggtaaat gatataagta atgacccaag cagacaggta tttaaaattc
     3721 aaaggattgt gctgataaat ttctatgttg aattgataat tctgcaacat caaaagagcc
     3781 gtaatagcca aggttaggaa aaaacaaccc aaaatcgtaa aatgaaaacg actatagtag
     3841 gtcactccca gataacgggc acgattgaaa aagtaaaatg tccctatgat gataacgatt
     3901 agcagcatat tagaattaaa aaggcttggt gctaatactg aaatgatata agataggagc
     3961 gacaaagcga tgcagatata gaaactttca gagcccgctt tattgaacag ttgttcttct
     4021 ctttcgtcta gtaattgata ataataaaat ctatttttca tcttcttcct cccaaaatag
     4081 ttggtctagg gttttcccta aacatctgca aatggactgg cagagcgaga gactgggatt
     4141 gtatttcccc gcttctatca aaccaatagt ctggcgcgtc accccgacag cctctgccag
     4201 ttgaccttgt gttaaatcac gctctacccg agctaatttt aattttaaat ttttagccac
     4261 cttcgtcctc cttatagttt taatactcat ctactcttaa aaaatccaaa accaacacaa
     4321 gctatcagct atttattata accccattaa tttgcactcc agtattatct tcagccgaaa
     4381 atcagtattt taggttgttt gaaataaact tcctagttta ctcttttatt tttatttagt
     4441 attagcttta gtaggatctt ttaaacgagt ttttattgct tatatttctt gtt
//
LOCUS       DAVLVF010000107         4462 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVLVF010000107 DAVLVF010000000
VERSION     DAVLVF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.107"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            167..1369
                     /locus_tag="VYN66_001833"
     CDS             167..1369
                     /locus_tag="VYN66_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1495813.1"
                     /translation="MKILFVNMFYNEAGGAQKSTKFLAEQLVKEGHEVFAYSDDAIDS
                     DIDEVINRVKIFRRRTPLFSLHYIFSAKKNPAKHFFYKIFETYNVFAKRKFINIIEEV
                     QPDIVHFNTISGMSLSIVKEAKKRNIRTVWTLRDYWLEYPWGEKDISLIKLLNRIYRP
                     FVKKSLRHLDIVTAPSAFTLNNFINKGFINPSSQKCMVIENAVIFSKGKLENIIKQKQ
                     ETKRNPEKTNFLFVGSILEIKGVFNLVKAFKKLTSPEISLKIVGKGKDLEKLQKEIES
                     DPRIQYLGFQDTETLYKTYQNSDVLVVPSAWDEPFGRVVIEGNANGLPVITSDRGGLP
                     EIVQTVGGGEIFTDESGNTLYSLLKKFVEMENYNVYYQCILENIDIYSIEKQSEKFTN
                     LYLDKEIE"
     gene            1403..2635
                     /locus_tag="VYN66_001834"
     CDS             1403..2635
                     /locus_tag="VYN66_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV1495814.1"
                     /translation="MKIAVAGTGYVGLSIAVLLAQHHEIKAVDIVAEKVELINQKKSP
                     IQDDYIEKYLVEKDLNLEATMDAKYAYEDAEFVVVAAPTNYDSKKNFFDTSAVEAVIQ
                     QVKKINPKAVIIIKSTIPVGFTESVQKKYQYKNILFSPEFLRESKALYDNLYPSRIIV
                     GTDLEDTYLKDKANIFASLLKEGAIKEDIDTLFMGVTEAEAVKLFANTYLALRVSYFN
                     ELDTYAEVKGLDAKAIIDGIGLDPRIGNHYNNPSFGYGGYCLPKDSKQLLANYFDVPQ
                     NMMTATVESNRTRKDFIAQRILEKAGVSETDSLDAFKNIQDIVIGIYRLTMKSNSDNF
                     RHSSIQGVMKRLKAKGVTVIIYEPTLKDGETFFGNKVVNNLDKFKEASNVIVANRFEP
                     SLEDVSNKVYSRDIFKRD"
     gene            2854..3608
                     /locus_tag="VYN66_001835"
                     /pseudo
     CDS             2854..3608
                     /locus_tag="VYN66_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            4003..4389
                     /locus_tag="VYN66_001836"
     CDS             4003..4389
                     /locus_tag="VYN66_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase"
                     /protein_id="HEV1495815.1"
                     /translation="MFAWFLFYEVGSKKDYVSYINPGLWGIAFIVCVLVAFAIFPKME
                     KNFPKTFLYFTKYGKDSLGIYILHAPICSMIRILMLKVGINSVFLHVVVGIVLGWYLS
                     ILATYILKKIPFLNIVLLPQKYIKLK"
BASE COUNT         1520 a          628 c          829 g         1485 t
ORIGIN      
        1 tccctatatt gtgtttaatt tgtttatctc ggattttgta aacgacctta ttttacattt
       61 atcagttcag tttgttattt tatttttaat atggggaatt gggatgtata ttagcggaga
      121 gtatcgacga gtcttcgcgg ttataagaaa ataaaggagc aattttgtga aaattttatt
      181 tgtgaatatg ttttataatg aagcaggagg tgcacaaaag agcaccaaat ttctagctga
      241 acaacttgta aaagaaggac atgaggtatt tgcatactct gatgatgcca ttgattcaga
      301 tattgatgaa gttattaata gggttaagat ttttagaaga agaaccccgc tttttagtct
      361 tcactatatt ttcagtgcta agaagaatcc agctaaacat tttttctata aaatatttga
      421 aacctataat gtatttgcga aaagaaaatt tatcaatatc attgaggaag tacaaccaga
      481 tattgttcac tttaatacaa tttcaggaat gtctttatca atagttaaag aagctaaaaa
      541 gagaaatatc aggaccgttt ggaccttgcg agactattgg ctagaatatc catggggtga
      601 gaaagatatt tctcttatca aacttctaaa tagaatatat agaccttttg ttaagaaaag
      661 tttaagacac ttagacatag taacagcacc atcagcattt actttgaata attttatcaa
      721 taaaggattt attaacccat cttctcaaaa atgtatggtc attgaaaatg ctgttatatt
      781 ttcaaagggt aagttggaaa atattatcaa acaaaagcaa gaaacaaaaa gaaatccaga
      841 aaaaacgaat tttctttttg taggttctat acttgaaata aagggcgttt ttaacttagt
      901 aaaggccttt aaaaaactaa ctagtccgga aatttctttg aaaattgtag gaaaaggaaa
      961 agatttagaa aaacttcaaa aagaaattga gagtgatcct agaatacaat atttaggctt
     1021 tcaagataca gaaaccctct ataaaactta tcaaaattcg gatgtattgg tcgtcccatc
     1081 tgcttgggat gaaccgtttg gtagagttgt aattgaagga aatgcaaatg gccttcctgt
     1141 catcacttca gatagaggtg gattacctga aattgtgcaa acagtaggag gaggagaaat
     1201 ttttacagat gaatcgggaa atacattata tagtctttta aagaaatttg tagaaatgga
     1261 aaactacaat gtctattacc aatgcattct tgagaatatt gatatttatt ctatcgaaaa
     1321 acaaagtgaa aagttcacta atctgtattt agataaagaa atcgaataat aagttgtttt
     1381 tgtagcagta acggaggtaa ttatgaaaat agcggtagca ggaacaggtt atgtaggatt
     1441 atcaattgct gtgttgttgg cacaacatca cgaaataaaa gcagttgata ttgttgctga
     1501 aaaagttgaa ttaatcaatc aaaaaaagtc tccaattcag gatgattata ttgaaaaata
     1561 tttagtggaa aaagatttaa atttggaagc aactatggat gctaagtatg cttatgaaga
     1621 tgcagaattt gttgttgttg cagcaccaac aaattatgat tcgaaaaaga acttttttga
     1681 tacatcagca gtagaggcgg tcattcaaca agtaaaaaaa attaatccca aagcggtaat
     1741 aattattaaa tctacaatcc ctgttggatt cacagagtcc gttcaaaaga aatatcagta
     1801 taaaaatatc ttatttagtc cagaattttt acgcgaatct aaagcattat acgataacct
     1861 gtatccaagt cgcatcattg ttggaactga cttagaagat acttatttaa aagacaaagc
     1921 taatatattt gcttccctac taaaagaggg ggctattaaa gaagacatag atacactttt
     1981 catgggagta acggaagctg aggcagttaa attatttgcc aatacctacc ttgcgttgcg
     2041 tgtttcttac tttaatgaat tggatactta tgcagaggtg aaaggtttgg atgcaaaagc
     2101 cattattgat ggtattggcc tagatccacg tataggaaat cattataaca atccatcctt
     2161 tggctacggt gggtattgtt tacctaaaga tagtaagcag ttattagcaa attactttga
     2221 tgtaccacaa aatatgatga cagcaactgt tgaaagcaat cgaacgcgta aggattttat
     2281 cgctcaacgg attttagaaa aagctggagt atctgagact gatagtttag atgcgtttaa
     2341 gaacatacaa gatattgtta taggtattta tcgtttaaca atgaaaagta attcggataa
     2401 cttccgtcac tcctctattc aaggcgttat gaaacgattg aaagctaagg gagttacggt
     2461 tatcatctat gagccaactt tgaaggacgg ggaaacattc tttggtaata aagtggtaaa
     2521 taatctagat aaatttaaag aagctagtaa tgttattgta gccaatcgtt ttgaaccttc
     2581 actagaggat gtgtctaata aagtttatag tcgagatata tttaagcgtg actaataaat
     2641 acgtccacgt gacgtaatat ctattaaatg aataaaatat taataaacat ggcatcgata
     2701 tcttttttgg acttgaattg tcaaattaag tgcacatttt tttcatagaa aacttcataa
     2761 ggtgttttcc ggttaagaca ttttcgtggt ctattatcta agttggttta tataatagac
     2821 ttcctgcgaa acaaaatatg gtataatagt tctatgaatt atgaagcaag taaacaacta
     2881 accgatgtac gatttaagcg tcttgttggt gttcaacgca cgacttttga agagatgtta
     2941 gctgtattaa aaacagctta tcaacttaaa cacgcaaaag gtggacgaaa acctaaatta
     3001 agcctagaag actttcttat ggcgactctt caataagtgc gagaataccg cacttatgaa
     3061 caaattgcgg ctgattttgg tattcacgaa agcaacttaa tccgtcggag ctaatgggtt
     3121 gaagtaactc ttgttcaaag gtggtgttac gatttcaaga actcctctca gttctgagga
     3181 cacggtaatg attgatgcga cggaagtaaa aatcaatcgc cctaaaaaaa gaattagcga
     3241 attattctgg taaaaagaaa tttcacgcta tgaagactca agcgattgtc acaagtcaag
     3301 ggagaattgt ttctttggat atcactgtga actattgtca tgatatgaag ttgttcaaaa
     3361 tgagtcgcag aaatatcgga caagctggta aaatcttggc tgacagtggt tatcaagggc
     3421 tcatgaagat atatcctcaa gcacaaattc cacgtaaatc cagcaaactc aagccgctaa
     3481 tagctgaaga taaagcctat aaccatgcac tgtctaagga tagaagcaag gttgagaaca
     3541 tccttgccaa agtaaaaacg tttaaaatgt tttcaacaac ctatcgaaat catcgtaaac
     3601 gcttcggata ttttaggcat actatatatt ttttattcaa ttattcactt ttgtattcaa
     3661 aaggttgcgg gtgcctctgt ccgagtaccc actatgatat ttaacctatt gaatatctat
     3721 aaatttcctc tgggagtttc atggtatcta tatacgttat ggtcgatatt gatagtttat
     3781 ggtttactat ctgttgtttt caaaaatcgt aagtcccttt tgttagttag tgtttttgcc
     3841 tatattttca cactatttat tcaaactgat atttttattg tgcaacggac gttggtttgg
     3901 gggatttgtt tctttcttgg cagtgtattg agtgaaattc actttgataa aattaatttg
     3961 aaaaaatttc tttttttctt tgtgttattt gattttattt atatgttcgc ttggttcttg
     4021 ttttatgaag tagggtctaa gaaggattat gtaagctata ttaacccagg tttgtggggg
     4081 attgctttta ttgtctgtgt attagttgct tttgcgattt ttcctaaaat ggagaaaaat
     4141 tttcctaaaa ctttcctata tttcactaaa tacgggaaag atagtttagg gatctatatt
     4201 cttcatgcac caatttgtag catgattcgg attctaatgt tgaaagtggg aataaactca
     4261 gtttttcttc acgttgttgt tgggattgtg ctaggctggt atttatccat actagcaact
     4321 tatatattga aaaaaattcc atttttgaat attgttttat taccacaaaa gtatattaaa
     4381 ttaaaataaa ttattttaga tttataatgt ggctctttgt caactaacat ctggagagga
     4441 caatcactgt cttctctttt tt
//
LOCUS       DAVLVF010000108         4077 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVLVF010000108 DAVLVF010000000
VERSION     DAVLVF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.108"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            661..1653
                     /locus_tag="VYN66_001837"
     CDS             661..1653
                     /locus_tag="VYN66_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1495816.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQKIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1655..2473
                     /locus_tag="VYN66_001838"
     CDS             1655..2473
                     /locus_tag="VYN66_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV1495817.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2475..3260
                     /locus_tag="VYN66_001839"
     CDS             2475..3260
                     /locus_tag="VYN66_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1495818.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3358..3576
                     /locus_tag="VYN66_001840"
     CDS             3358..3576
                     /locus_tag="VYN66_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV1495819.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3561..3722
                     /locus_tag="VYN66_001841"
     CDS             3561..3722
                     /locus_tag="VYN66_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1495820.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNP"
BASE COUNT         1130 a          696 c          889 g         1362 t
ORIGIN      
        1 attacaaaca gaatatgaaa gctttttata tcactattga aaaatttata cgagatgatg
       61 aaagccttaa gtgttatttt acaaaggtta tttcaagtcg ttccaaggta acaagtctag
      121 atcagattga agctgataaa acgatacaaa gaaaatattc aagtgagcta aaaaaattta
      181 ttggatttta taatgagatt atttgtgagg aaaatagttt cctacatgta cgaaagaggt
      241 ggtcgagttg gtttaggtag tcggtgcgtg agttgataat tctcagggta tggacttctt
      301 tttcatgaat gaggtaaaag agcaggtatt gtttagagac aatcattctg agcatatttt
      361 ctggatagag ggagtatccg attttatgat caaagttaat accgccctct ggtgagaaga
      421 tgagtaggtt ggtaatttaa actattaaac agaatttttg attaaaagta ttatttcatg
      481 agagaaatcc taatttcaca atctataggc aaacgcttgc atttcgtttt ttattggact
      541 ataataggtt ggtataaagc cttctgtagt aataaaatgt agaaggtgta gaaagtaagg
      601 atttagaata tttgtagtta aaaacacaat gttgctattc cttacgatag ggagatagat
      661 atggcaatga tagaagtgga acatcttcag aaacattttg tgaagattgt taaggaaccg
      721 ggcttgaagg gggctttgcg ctcctttatt catcctgaaa agcagacctt tgaagcggtc
      781 aaggatttga cctttgaggt tccaaaaggg cagattttag gatttatcgg ggcaaatggt
      841 gctgggaagt cgacaaccat taaaatgctg acaggaattt tgaaaccaac atctggtttt
      901 tgtcggatta acggcaagat tccccaggac aatcggcaag attatgtcaa agatattggc
      961 gtagtctttg gacaacgcac ccagctatgg tgggatttgg ctctgcaaga gacctacact
     1021 gtcttaaaag agatttatga tgtgccaaac tcgctctttc ataagcgtat ggactttttg
     1081 aatgaagtct tggatttgaa ggactttatc aaggatcccg tgcggactct ttcactggga
     1141 caacggatgc gagcggatat tgcggcctcc ttgctccaca atcccaaggt tcttttttta
     1201 gatgagccga ccattggttt ggacgtttcg gttaaggata atattcgtcg ggcaattact
     1261 cagatcaatc aagaggaaga aactaccatt cttttgacca ctcacgattt gagtgatatt
     1321 gagcaacttt gtgatcggat tttcatgatt gacaaggggc aaaagatttt tgatggaacg
     1381 gtgagccaac tcaaggagac ctttggtaag atgaagactc tctcttttga actgctacca
     1441 ggtcaaagtc atctcgtctc tcactatgaa ggtctgtctg atatgaccat tgatagacaa
     1501 ggaaacagcc tcaacattga atttgatagt tctcgctacc agtcagctga cattatcaag
     1561 caaaccctat ctgattttga aatccgcgat ttgaagatgg tggatacgga tattgaggat
     1621 attatccgtc gcttctaccg aaaggagctc taggatgatc aaattgtgga gacgttataa
     1681 accctttatc aatgcagggg ttcaggagtt gattacttac cgagtcaact ttattctcta
     1741 tcggattggc gatgtcatgg gggcttttgt ggccttttat ctctggaagg ctgtctttga
     1801 ttcttcgcaa gagtctttga ttcagggctt cagtatggcg gatatcaccc tctacatcat
     1861 catgagtttt gtgaccaatc ttctgactag atccgattcg tcctttatga ttggggagga
     1921 ggtcaaggat ggctccatta tcatgcgttt gttgcgacca gtgcattttg cggcctccta
     1981 tcttttcacc gagcttggtt ccaagtggtt gatttttatc agcgttggcc ttccattttt
     2041 aagtgtcatt gtcttgatga aaatcatatc gggtcaaggt attgtagagg tgctaggatt
     2101 aactgtcctt tatcttttta gcttaacgct cgcctatctg attaactttt tctttaatat
     2161 ttgctttgga ttttcagcct ttgtgtttaa aaatctttgg ggttccaacc tacttaagac
     2221 ttccatagtg gcttttatgt cggggagttt gattcccttg gcattctttc caaaggttgt
     2281 ttcagatatt ctctcctttt tgcctttttc gtccttgatt tatactccag ttatgatcat
     2341 tgttggaaaa tacgatgcca gtcagattct tcaggcactc cttttgcagt tcttctggct
     2401 cttagtgatg gtgggattgt ctcagttaat ttggaaacgg gtccagtcct ttatcaccat
     2461 tcaaggaggt tagtatgaaa aaatatcaac gaatgcatct gatttttatc agacaataca
     2521 tcaaacaaat catggaatat aaggtggatt ttgtggttgg tgtcttggga gtctttctgg
     2581 ctcaaggctt gaatctcttg tttctcaatg tcatctttca acatattcca ttcctagaag
     2641 gctggacctt tcaagagata gctttcattt atggattttc cttgattccc aagggaatgg
     2701 accatctctt ttttgacaat ctctgggcac tagggcaacg cctagtccga aaaggagagt
     2761 ttgacaagta tctgactcgt cccatcaatc ctctctttca catcctagtt gaaacctttc
     2821 agattgatgc cttgggtgaa ctcttagtcg gtggtatttt attgggaaca acagtgacca
     2881 gcattgtttg gactcttcca aaattcctgc ttttcctagt ttgtattcct tttgcgacct
     2941 tgatttatac ttctcttaaa atcgcaacag ccagtatcgc cttttggact aagcagtcag
     3001 gcgccatgat ttacatcttc tatatgttca atgactttgc taagtatccg atttctattt
     3061 acaattctct tcttcgttgg ttgattagct ttatcgtgcc tttcgccttt acagcctact
     3121 atccagctag ctatttctta caggaaaagg atgtgttctt taacgtagga ggtttgatgt
     3181 tgatttctct ggttttcttt gttatttccc ttaaactttg ggataagggc ttagattcct
     3241 acgaaagtgc gggttcgtaa aagctaaagt aagactaaaa tcaagaaagg aacttatgat
     3301 gtttgtaatt gaagaagtca aggatgaaaa tcaaaaaagg cagttgtcgt tgaggttttg
     3361 aaggatttgc cagaatggtt tggaatccca gaaagcacac aagcctatat agaaggaacc
     3421 acgacactgc aagtttggac cgcctatcag gagagtgatt tgactggatt tgtaaggtta
     3481 tcctattcga gtgaagattg tgcagagatt gattgtctcg gcgtaaaaaa gcttatcaag
     3541 gtagaaaaat tgggagccaa ttgcttgcta ctttagagag tgaagctcgt aaaaaagttg
     3601 gttatctgca ggtcaaaaca gtggcagaag gttctaataa agattatgat cgaacaaatg
     3661 acttttatcg aggtcttggc tttaaaaagt tagagatttt ttctcagcta tggaatccgt
     3721 aaaatccttg tcagattttg attaaaaagc ttgaataata ttacttgaca tctattctca
     3781 gagtgccata ctgtaagtgt aatcgccgat ttagctcagt tggtagagca aggcactcgt
     3841 aaagcctagg ttataggtag ataaacgact gaggagttga aaaaatagat aggtagaaga
     3901 taaccgttaa gccttattct tagcggttat ttatattgtt taatagcgct gatatttcat
     3961 caattatgcc tgttttcgtg tttctggtag ttgttcaagt ttattgctac tatttttgat
     4021 ggtatgaatg tgcttataat gtatcccggt taacgaaagt tttggactta tactcat
//
LOCUS       DAVLVF010000109         4076 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVLVF010000109 DAVLVF010000000
VERSION     DAVLVF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.109"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            110..1519
                     /locus_tag="VYN66_001842"
     CDS             110..1519
                     /locus_tag="VYN66_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1495821.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKSIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            1537..2832
                     /locus_tag="VYN66_001843"
     CDS             1537..2832
                     /locus_tag="VYN66_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1495822.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVNRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            2837..3145
                     /locus_tag="VYN66_001844"
     CDS             2837..3145
                     /locus_tag="VYN66_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1495823.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            3142..3450
                     /locus_tag="VYN66_001845"
     CDS             3142..3450
                     /locus_tag="VYN66_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1495824.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1377 a          679 c          768 g         1252 t
ORIGIN      
        1 aagaaatgag gcaaacacaa gatatgtatc aacaattttt aaaaatgtct agacaaattt
       61 taaaattatg ctatacttta tgtatagaat aatagaaagg aggttgagca tgaaaaaaaa
      121 gttagtattt cctaatctgt tttggtgggg agcagcttct agcggacctc agacagaagg
      181 tcaatatgga aaagtacatg aaaatgtgat ggactactgg ttcaaaacgc atccagaaga
      241 ttttttcgat aatgtcggac ctcttgtagc cagtaacttt tttcatactt acaccgaaga
      301 tttccacttg atgaaggaaa ttggagttaa ttctttccgc acttccatcc aatggagtcg
      361 actcatcaag aatttagaga caggtgagcc tgatccaaaa ggtattgctt tctacaatgc
      421 catcattgaa gaagctaaaa agaaccagat ggatcttgtg atgaatttac atcattttga
      481 tttaccagtg gaacttcttc aaaaatacgg tggttgggaa agcaaacatg tagtggagtt
      541 attcgtgaag tttgccaaga ctgctttcac atgctttgga gataaggttc attactggac
      601 aactttcaat gagccaatgg tcattccaga agcaggatac ttatatgctt tccattatcc
      661 aaatctaaaa ggaaagggaa aagaggccgt acaagtcatc tataatctaa accttgctag
      721 tgcaaaagtg attcaactat atcgctcatt agaacttgat ggaaagattg ggattatttt
      781 aaacttgaca cctgcttatc caagaagtaa ttctccagaa gacttagaag caagtcgatt
      841 tacagatgac ttctttaaca aagtcttctt gaatccagct gttaaaggaa ctttcccaga
      901 aagattggta aaacagctag agagagatgg cgtgttatgg agtcataccg aaaaagagct
      961 tcaactgatg aaatcaaata cggttgattt tcttggagta aactactacc atccaaaacg
     1021 tgttcaagca caagcaaatc ctgaggaata tcagacgccc tggatgccag accaatactt
     1081 caaagagtat gaatggccgg agcgtcgcat gaatccatat cgtggttggg aaatttttcc
     1141 gaaatccatt tatgatattg ctatgattgt gaaggaagaa tatggtaata tcccatggtt
     1201 tatcagtgaa aacggaatgg gtgttgaaaa cgaagcacgg tttatcgatg aaaatggagt
     1261 tatcgatgac gtgtatcgta ttgaatttta tgaagaacat ttaagatggc tacataaagc
     1321 cattgaagag ggaagtcact gttttggata ccacgcttgg accgcatttg attgctggtc
     1381 ttggaataat gcatataaga atcgttacgg atttatctcc gttgatttag aaacgcaaaa
     1441 gagaaccatc aagagctcag gaagatggta tcgcaaagta agtgacaata acggttttga
     1501 agtagaaatt gaggagtaaa gagaggagat attcatatga aaaactcaaa atttatagac
     1561 caatttgcca cctttgctgg taaactaggg aaccaaattc atttaaaaac cctaagagat
     1621 gcattcgtaa cagtaatgcc attatatatt ttggcaggtt taatcgttct tttgaacaac
     1681 acggtattta agtggatttt ccaaggggat acattaacaa gattccaata ttggggaata
     1741 acaattgcaa acggtacttt aagtatttca ggtatgatta ttgctgtaat ggttggttat
     1801 ttcttagcta aaaacagaga tttcgaaaac ccgttagcag catcaatgct atcattagtt
     1861 tctttaattg tgatgatgcc aaatacagtt tctgtagttc ctgacggagc aaaagatgct
     1921 gtaaacattt caggtgttct ttcattcaac aacacaggta caggcgcaat gttcgccggg
     1981 gttatcgtag cgattattgc aacagaatta ttcattgaat tatcaaatgt taaagcttta
     2041 caaatgaacc ttggtgaaaa tattccacca gctgttaata gatcatttag cgtattactt
     2101 ccagtcatga ccgtcatctc cttatttggg gttgtttcag cattattatt caatataact
     2161 ggaatgaact taatctcaat cattacaatc tttattcaag aaccaattcg tcatattggt
     2221 acaagcttaa tcggggtcat tattatttac tctttaggaa atatgttatg gctatttggt
     2281 attcaccaag cagttattta cagtgccatc ctagaaccat tactattaat taacattact
     2341 gaaaacatca ctgcagcaaa taatggacaa gccattccac acatcatcaa cctatcacaa
     2401 atacaaacat tcgctttaat gggtggtagt ggatctacat tatgtttatt aatagcaaca
     2461 ttcttagtga gtcgcaatgc tgtctctaaa aacgtggcta aattatcttt tggacctggt
     2521 atcttcaata tcaatgaacc agtattattc ggttacccaa tcgtttataa catttcatta
     2581 gctattccat ttatcacagt tccagtcctt ggtattttaa tcagctactt agcaacagtt
     2641 acaggattca tgagtcctgc atttatacaa gttccttgga ctacaccagt attcttaaat
     2701 gcatggttag caacagcagg ggacgtgaga gcagttctag ttcaattcat catctttgca
     2761 cttggagttc ttctatacat tccatttatc aaagttaatg acaaagttgt tgaacaagaa
     2821 atggaaggtt aagattatga aacaaatttt attagtatgt aatgcgggaa tgtcgacaag
     2881 tatgcttgtt aaaaaaatgc aacaaagtgc gactgaacgt gggattgaaa tctctatcca
     2941 agcaaaatca atgacagaag ctaaaaagaa tatccatgaa gcagatgtca ttctaattgg
     3001 gccacaaatt cgttatgagt tacttgctgt gaaagaaatt gcaggaaata tccctgtaga
     3061 taccattgat atgcgggatt acggaatgat gaatggagca aaagttctgg aacaagcact
     3121 tgcatggata ggagaaataa gatgacagaa gaaatggaat atatctgttt tcagctcatt
     3181 gccaatagtg gagcagccaa atcatccttt attgaagcca tccaactagc taaggcagga
     3241 aatttaaaag aagcgaaaat taaagttgaa gaagcggaag actccttagt agaagcgcac
     3301 aagattcact ctaatctcat acaaaaagaa gctactggag aaaaaatagg attctccttg
     3361 ctatttatgc atgcagaaga ccaaatggct tcaacagaga ttattcagtt gctatctaaa
     3421 gaattcatcg atctctatca aaataaatag gaacactgtc attcccatct gtctttatta
     3481 atgacgacta cattgtagga acctccaaaa gttggacaaa atgattgaaa ggattggatc
     3541 ctgcattaca caagatccaa tccttttctt ttactctagt ttttataata tgttgaatct
     3601 agaatagtcc actgtagttt ctaaaaaatt gctggaaatg gatgttaagc tccaatttat
     3661 ttgtttatat cttatttcag tttactatac tagcttatta ttctagtgaa aaattgaatg
     3721 agccactatt tatttaatat gtatgatgag ggaatgactc aaaaacttct tgtcgatata
     3781 agaatatctg agatagtatt ttaaaacgaa aggactataa aagctcattt ccttcgcact
     3841 tattttatag agatctatca ttatactctt tagaaatcaa aaagcaacac accttgtcat
     3901 cgttttatcg taaatctgtt aatgaggggg ctagtcttat ctgcaatctc aaatcagtgt
     3961 tttgagctga atggcactcg ttttctagtt tgctctttga ttttaattgg gccttagtaa
     4021 atactactct taaattaaac tatagtgaat tgaaataaga tgtgaacaac tctatc
//
LOCUS       DAVLVF010000110         4066 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVLVF010000110 DAVLVF010000000
VERSION     DAVLVF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.110"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(131..1546)
                     /locus_tag="VYN66_001846"
     CDS             complement(131..1546)
                     /locus_tag="VYN66_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1495825.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1559..3397)
                     /locus_tag="VYN66_001847"
     CDS             complement(1559..3397)
                     /locus_tag="VYN66_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1495826.1"
                     /translation="MSYKDKVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3415..>4066)
                     /locus_tag="VYN66_001848"
     CDS             complement(3415..>4066)
                     /locus_tag="VYN66_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV1495827.1"
                     /translation="INVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHISSSIER
                     YKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHFVNANL
                     ENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVENTTYCD
                     DDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTKNLD"
BASE COUNT         1367 a          812 c          532 g         1355 t
ORIGIN      
        1 acaaaattaa attaattaat ccccctataa aataaagagg tttagaattt gatgtcttcc
       61 aaacctcttt atttcatatt taatgagatg ctgccttaac cgtgataata tctaatcatc
      121 aataaacaaa ctatttgaat aaggattctc catttgattc aatcactcct ttataccaag
      181 taaaggacat tttcttatat cgattgaatg tgccatttcc atcatcattt cgatcaacat
      241 aaatgagacc gtacctctta gaaagctgtg cagtggacat agaaacgcag tcaatacatc
      301 cccaagacgt atagcccata atttccacac catcttgtag agcttcagct acttgcaata
      361 aatgttcttt catatactga attctataat catcttggac tgttaagtta ttaaattcat
      421 cttttattag ttgatcttta gcacctaatc cgttttccac tataaataat ggaatttgat
      481 aacggtcata atatctattt aaaattatac gtagtccaat tggatcaatt tgccatcccc
      541 actcagaaga ctctaaataa ggatttacta aaccaccaat aatattccct tctcctgaat
      601 tatactgtgt tggaagagca gattgagtca cactcatgta atagctaaag gataaaaaat
      661 ctacagtata attttttaat aactctgcat cttcagctgc aaactctatg ttaatgtcat
      721 tttccttaaa atatcttttt gcataattcg gataataacc tctaacatgc acatctgaaa
      781 atagataatt tagattctca tactcatgag tcgcccatac atcttttgga tttggagtca
      841 ttggataagc tggcatagct aataccatac accccacctt aaactctgaa ttaatctcac
      901 gagcaatttt tgtaaccaaa ctcgaggcga ctaattcatg atgtatagct tgatataatt
      961 cttgtttcga aagattctcc ttaggtatat ctattcctcc actagtaaac ggtaattcca
     1021 aaacagagtt tacttcgtta aatgtaagcc aatatttaac tttatcttta tacctttcta
     1081 aaactgttcg agcaaatttt tcataaaaat gaatcattct cctatcaatc catccatgat
     1141 attttcttgc taaatataat ggagtctcat agtgtgaaag agttacaagt ggttctatcc
     1201 cgtgagcatg tagttcatca aacaattcat cataatattt caacccagct tcgttaggtt
     1261 cttcctcatc tccttttgga aaaattctac tccatgcaat agaagtacga aaaacattaa
     1321 agcccatttc agaaaacaag gatatatctt ccttatattt atgataaaaa tcaataccta
     1381 tcaattttaa gttatcttct gtaggatttt ctgttgcttc tcctaatcca cctttgggta
     1441 acacatcctg aactgataag cccttaccat cttcattata tgctccctct acttgattag
     1501 ctgcaacagc tccaccccaa agaaaatcat ctggaaaaat ggtcataact ttcctccatt
     1561 ataatattac cagtaattcc ttagaatgct cgattgtctg attattaggt aatactaata
     1621 catctagaaa atcattggta ttcgttacaa ttactggtgt aactgtttcg tagcctttag
     1681 tcttgattaa attcaagtcc atttcaaaaa tcaactgatt tttgaaaact ctgtctcctt
     1741 cttctacatg actaataaaa ccttgacctt ttagctcaac agtatctaat ccaatatgaa
     1801 ttagtaactc aacaccctca tcactcttca atccaattgc gtgcttagtc ggaaaaatat
     1861 ttgtaatttt cccatcaaat ggtgcataaa ccttaccttc acttggaata atcgctactc
     1921 cgtctccaat tagtttatct gaaaatgttt tatcctggac atcgcttaac ggaatgattt
     1981 ctcctgatat aggagaaaat atcatttttt tatttgaaac tccagcttta acttctaaat
     2041 tgctagaact ctcttcttca tcgattccaa atatataagc taatacaaag gtaataacaa
     2101 ccgaaataac cgccacaatt aaagcattta caatatttga tggcacatca gaataaataa
     2161 attgaggcaa cgctatcaaa gatgggacag caaatagata cgctttaaca ctagtaagac
     2221 ctgcaaataa tcccgctaat ccaccaccaa tcatagctgc ataaagcggt tttttatatt
     2281 ttaaagtcac accatataat gcaggttcgg taatccctgc aagtaaggct gagaaacctg
     2341 ctgcaaaagc aatttgtttt gtattattat ttttactctt taatgcaaca gccatcgaag
     2401 cagccccttg agctaagttt gaccctaaca ttgctggaag aattaatacg tctggagtag
     2461 caatagatgc cgccaaaaaa ataggtgcaa aagcccaatg cattccagtc ataacaataa
     2521 atggcataat agcaccaaga atagctaatg taagccatcc agctacacca tacatttgcc
     2581 caactagatt tgataatcct tcaccaacaa ttactccaat aggcccgact acaactaagg
     2641 caatacagct tgatactaat aatactagcg taggttgcaa aaaactctta gtaacagctg
     2701 gtgttaattt agcaatcatt ttttcaatat atttcatcaa ccaaaccata ataagaattg
     2761 gaacgactga cgaaccataa ctagctggtg tcacaggtac accaaataaa ctaagaggat
     2821 tccctgattg caccatttga acaaaatttg gatggagaag tacacctgct acagacatag
     2881 ctaatgtaga tgttactttt aatttttgtg atgcagaata agctaataac agcggtaaga
     2941 aataatatgg agcatcccca aaaaatgtca aaaaagcaat agtctgagaa tctgattgca
     3001 atataccaag cattggtaaa atgattacca agactttcaa catacctccc cctaacattg
     3061 ctggaatgat tggagtcatg gaaccagcga tatactcaat gattctttct aaaatattcc
     3121 ctttgtgccc ttgaacaact gaatcggatt caaaattgcc aagtttaacg aattctttat
     3181 aataattagc tacatcatta ccaagtataa tttgatattg tccattcttt ttcataatac
     3241 ctattacacc tggtatcttc ttcacatcat catcattgac taaattttca tcttttaatt
     3301 ctaatcttaa acgtgttaca caatgggtaa ctctattgac atttttttca cctccaatta
     3361 catcgaggat tttttgtacc ttatctttat aactcatggt attctcctat tctattaatc
     3421 taaatttttt gttaagcgac gaatatgagc catcaaataa actaattcac tagaagtcag
     3481 caaataattg tactcagttt gtataaacat tgccacctgt tcaccacatt catattctct
     3541 aggatattta tttttcatta atgctaacaa gtcttcatca tcatcgtcac aataagttgt
     3601 attttcaacc aagcgatggg caaataattt tatatgagtt atgaatctat aataatcaat
     3661 agaatcttgg ttaaactcag tacaatagaa atcttgaatg attttctcaa ttcccataat
     3721 tatttcagtg attttatacg actcttgaaa attattttct aaattagcat taacaaaatg
     3781 tagcgctatg aatgcagctt cgtcaattgg aagttcaata cctaactctt tttttattat
     3841 ttgtaaagcc cttttcccaa gctcaaattc ttctttataa tatttcgaga tttcccagcg
     3901 taggggattc gaaataataa tcccttcttt ataacgttct atgctcgaag aaatatgatc
     3961 cgttaaatta atatagataa tttcatcaaa attattcccc aatgttattt tacctagatt
     4021 aattattttt tcggtgatgt acaccaactc caaaggaacg ttaata
//
LOCUS       DAVLVF010000111         4016 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVLVF010000111 DAVLVF010000000
VERSION     DAVLVF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.111"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            546..1409
                     /locus_tag="VYN66_001849"
     CDS             546..1409
                     /locus_tag="VYN66_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1495828.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGSNQKEIEHFIQSLQVLENPQLIALFE
                     LRLQQYKKLID"
     gene            1695..1895
                     /locus_tag="VYN66_001850"
     CDS             1695..1895
                     /locus_tag="VYN66_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEV1495829.1"
                     /translation="MLNLQFAETMELTEAELEIVYGGEFGNNAVIPAGAWGGFGTPWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            1962..2627
                     /locus_tag="VYN66_001851"
     CDS             1962..2627
                     /locus_tag="VYN66_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1495830.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            2699..3925
                     /locus_tag="VYN66_001852"
     CDS             2699..3925
                     /locus_tag="VYN66_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1495831.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICMINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT         1148 a          707 c          774 g         1387 t
ORIGIN      
        1 ttaaacgtta gtatagataa cgtaattttt gaaaagaatt ttgaactatt tacaaaacaa
       61 agtgatctaa ttgttgcaaa tagatgggat aggggccttg aagcttataa agataaagtt
      121 tacacaaggg ggatctggat aagagattaa ctattgatat taaaaaggca ataatttata
      181 aaatttgtac aaaaaaatgt taaattttca cattttcata gtatactgtt taatagaaag
      241 taggttcttt gtaaccgctc cattataaaa tcctttcact cttccggtat actattagga
      301 aaaacataac tggaggatat tttgagctca cacctcatcg ttccagtggc tgttccccaa
      361 gctcgtgtac tgcccaaaaa agttagacat tctatttaga acgaggattg agttctgtat
      421 tgtacaaggc tcggtccttt tagagtcagc ttaaggctgg cttttttatt ttttctaact
      481 atcagaatat tttgacaaat gaacacaaat aatgatataa tatgcaaaag ctaggaggtg
      541 gtaggatgat tgaaaaaatg gaattggggg aattttacaa ggaattacgt ttggcgagaa
      601 agctcaagca gtcagatgtg gcttgtgacg ggctaacagc ttctcagttg tccaagtttg
      661 agctagggca gtccatgttg tctgctgata agctcatatt agccatccaa gggatcaatg
      721 tgacctttga tgagtttggg cacaagctca acaactatca agaatctcca catatgcaga
      781 ttggtagaaa ggttgtggat cgctttgctc atcaagatat agctggctta gagcaactgt
      841 tggaggaagt cgagcaagaa cagatggcag agacttatcg tcgtttgaat gctattgtaa
      901 tcaaagatgc catccactca ctagataaaa gctacccact agcagaggag gatagcgagt
      961 ttttgaccac ctatctctat gctatcgagt cttggacctg gtttgaactc tatctctttt
     1021 gcaatactct gcccttcttg agcaatcaag atctgatctt tttatcaacc tccttactcg
     1081 aaaaatccaa agaatttaaa gagttagtac acaatcgatt gtatatgaag caaggactct
     1141 taaatatcat ctcagagctc atggagcgca aacttttttc ttatatccca atctttgaag
     1201 ccgagctgga gagtatgctc cgtccatacg atgtttttga gaaagtgttg tggcagtttt
     1261 taaagaagat gagcattttc cttcaaacta agggaagcaa tcaaaaagag attgaacact
     1321 ttatccaatc tctgcaagta ttagaaaatc cacaattaat agcccttttt gaattgcgtt
     1381 tgcagcaata caaaaagctt atcgattagt tagaaatggc aaaataataa taatctcagc
     1441 aacgattttc aataatgtta aaatttgttt cttcatattt ctccactcct cattagtagt
     1501 aactttatta taccagagaa gtaatcaaat tcaccaaaat cgcaaatctg aaatttctat
     1561 aagaaaaaat atcaaatatg cgattttcta aaataagcca attttcgtgt tatactgtac
     1621 ttgtaaagca cttgaagcaa atcctaggtc gcagaagtgg tttacaaatg aagataattg
     1681 aaggagtgta aaagatgtta aatttacaat ttgcagaaac aatggaattg acagaagctg
     1741 agttggagat agtttatgga ggggaatttg ggaataatgc tgttatccca gccggtgctt
     1801 ggggagggtt cgggacacct tggtcaatca ctaatttctg gaagaaaaat tttaacgata
     1861 gacctgactt tgattctgac cgccgtcgtt attaatatcc aaagcccttg ccttttcttg
     1921 ggtaggggct gtctttattt catagtatag gaggaaatag aatgaaaaag ataatctcac
     1981 accgctactt tattatagtt tttctactag ttattgctga ccagaagttc agtgttctag
     2041 ttttacgcag cgaccttgtt actgggctat ctgactttgc ctattatctg tcggatatga
     2101 tgttgaattt tcttgtggtt ttatttgctc ttattgctat gatttggtcg gggaaatggc
     2161 agaaaatcaa tagtagaaag tttaaaggat cctatctttt ctattccttc ctagctctgc
     2221 ttgcttttgt tgtttggaat ttcgttacct tttttctttt cccacctact cgaaatgaaa
     2281 tttcttatca acatgctgct ccgactttta caggggctac agcatttttg atgtattttt
     2341 tctatcctgt gattgcaggt cccatttttg aagagataat ccatcgggga ttggtgatga
     2401 cagctctgga aaaaggaaag aaatgggggc tagatgtact tggctctgct attttatttg
     2461 gggtctcgca cattagtaat cacggttggg tcttgacaga ctttgtcttc tatatgggtg
     2521 gaggtcttat atttgcagtc ttatttagaa tgacaaagtc aatttattgg cctattggac
     2581 tgcatatagt ctacaatggt attggtcagc ttttgatgtt actgtaattt tggttgttag
     2641 tagtatcgcg gtctattctt ggggaaagat tcccatttga acttaagaag gagttgtaat
     2701 gaaagtattt cttcaaaata gagattttag gcaattaact atcaaccagt ggatttcaac
     2761 gcttggggat acgatttttt atctggcctt cctgaattat gtggcagatg cgtcttttgc
     2821 ccctttggcg attttactta tcacgatttc agaaaccctt ccccaagttc tacaaatctt
     2881 tctgggagtt ttggcggatt ttcaacatca tcgtgtcttg aaatacacag tcattagttt
     2941 tacaaaattt ttgctttact ctatagtgtc cctatcgctt tcagggcagt ctttttcctt
     3001 gttgctagta acctttattt gtatgattaa cctcttgtct gacacattga gttatttttc
     3061 aggcgccatg ctcactccga ttttcattag aattattggg caagatcatc tggcagaagc
     3121 tattggattt aaacagtcaa ctgttagttt agtgaaaaca atcagtaata tcctaggagg
     3181 agtcttacta ggtattctat ccatccagtt tatttcctta ctgaacgctc ttaccttttt
     3241 aatcgcattt ttaggtatcc tcttcataaa aactgacctc ttgaaagtag aaaaaacgat
     3301 tagctatcaa gaaggactct ctgtaaaatc cttttgccag catttgctcc aatcatcgaa
     3361 attgatatgg aatatgaata aggtgctctt ggttttgttt attatctcta ctagtcaagc
     3421 agtgataaat gttacagttc ctgtctctac tctatttttg aggaatcaac cctttttgaa
     3481 tttacaaaca ggtcaatctc ttgccttgct atccactttt gaactgtcag ccctgattgt
     3541 cggaagcctt gtaagtggct atctgcaaca taccatttcc ataaaaacag ctctctatgc
     3601 ctcacttgtc atccagttgc ttcttctagt aggatttgcc acagttcgtt ttgactggat
     3661 tctcatcttt agtaccttgg atgccttttt cgcaggtgtt ctctctccta gattgcaaga
     3721 actcgtcttt aaacaaatac ctgaggagtc aatgggagca gttcaatctt ctatcagtgc
     3781 cattacggtt gttttaccta gcttatttac aatatctttg gtaaccattg ctactagctt
     3841 tggaactctg gcagttagct ttgttttatt gctatttctt ctagttgcct ttgttatgct
     3901 tctgaatatt cgtgaaagta tttagtggag aatttctata atatatgttt gagactggat
     3961 tgccagtctt tttatgatgg tcaaaatcaa cagacttttt ctcttttctg aatgtg
//
LOCUS       DAVLVF010000112         3994 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVLVF010000112 DAVLVF010000000
VERSION     DAVLVF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.112"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(19..2565)
                     /locus_tag="VYN66_001853"
     CDS             complement(19..2565)
                     /locus_tag="VYN66_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV1495832.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(2688..3128)
                     /locus_tag="VYN66_001854"
     CDS             complement(2688..3128)
                     /locus_tag="VYN66_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV1495833.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(3103..3972)
                     /locus_tag="VYN66_001855"
     CDS             complement(3103..3972)
                     /locus_tag="VYN66_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV1495834.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
BASE COUNT         1161 a          879 c          789 g         1165 t
ORIGIN      
        1 tttttatttt aggcttattt atgcatttcc gtattgaaga acaactgctt cgactgcagc
       61 tttttcacgg ctaatcaagt caacacgtgc tgcaatttcc ttgattccca taccgatgtt
      121 acggctaaga gcaaggtcag aaagttgcgg ctcaaagaac tccttgtatt ccgccaagcg
      181 ttgctgagtc ttaaatacat gagcaggaag gataacaaag ctatcaaagc tcatatctcc
      241 tccaagggct gccttaatcc aagcccagtt ttcacgcgcc caagaccaag ctgttttctg
      301 agttgcttga tgagctagga attggtaata ccaagcagac aagtcctgtg gtttgaccac
      361 aaatttgtcc ttccaagaag taatcaagtt ttggatatta tccgcatctg tactgtatgc
      421 aagagctgct gccaactggc gtttaaagac agcatctgtt gcgtgagtat aagtatcaag
      481 ataaagtgct aacaagtctt tagtctcatg atgtttcatc tcattaatca gaacttgtga
      541 acgaatagct gctgggagtt ctgcaagatt ctccttgtgt gttgcgaaga tttggctagc
      601 gacttgacga gcttctgcat catttgagcg aatcatcatc gaaacagcca gctgacgaac
      661 caattcatcc tcatctgatt ctccgtcttt agcttcaaaa ccaagacggt catagttatg
      721 acgagccaat ttagcaacca gtcctttgaa ggctgtttca gcatccgttc cttcatcaat
      781 aaagcgctca agggctgaaa tcacttgaga aacagctgaa accaccagat aagactcttc
      841 cttagcaagt ttatcaagaa ctggaagcaa gtctgcataa gaaatgtgcc ctgcctcagc
      901 caacaaacga cgttcttgaa caatttgcag tttgcttgtg ttatcaagtg tctctagctc
      961 agcaagaaca gcttctaaca agtctccttg atagtcggta atatagtggg cagtattttc
     1021 agtgttgaga cgaagagctc cttcattttc agcaagaaga gctgcgtagc cagggatttc
     1081 gatactttca gtttcgagtg tatcaggcaa gcctttccag ttgctattga ggggcaccac
     1141 ccagagacgg ttcttgtctt cgttctcacc gatgaagaat tgtttttgtg aaatcttcaa
     1201 gacatcattt tcaactttga cagtaagaac tgggtaacca ggctgttcca accaagaatc
     1261 catgaaggct gcgacatcac gtcctgacgc ttgaccaagg gcatcccaaa ggtcactacc
     1321 aatggtgttg ctgtattggt gtttttcaaa gtaggcatgc aaacctttag caaaatcagc
     1381 atctcctagc caacggcgaa gcatgtgcat gagacggctt cctttggcat agacgatagc
     1441 gccgtcaaag agtgtattga tttcatctgg atgtttaact tcgacgtgga cagactgaac
     1501 gccatcagta gcgtcacgtt caagagcaag aggtactcca cctgtttgga aatcttcaaa
     1561 gatattccag cttggttcga tggtatccac acagacgtat tccatcatat tagcgaaact
     1621 ttcattgagc caaaggtcat cccaccattt catagtcacg aggttcccaa accattggtg
     1681 agccaattca tgggccacaa caagggcaac ttgttgacgg ctagcaaatg tagagttctc
     1741 atcgacaacc aagtaaactt cacggtaggt cacaagaccc cagttttcca tagcaccagc
     1801 tgagaagtca ggaagggcga tgtggagaga ttgaggaatt ggatacttaa ctccatagta
     1861 atcttcgtaa aactcgatag agcgaacagc gatatccagt gagaaatcaa gatttgaaag
     1921 tggatgtgct ttggttgagt agacacctac cagggtacca tttttagttt tagcggtcac
     1981 cccttgcaaa tcaccagcaa caaaggccaa caagtaagaa gacatgcgag gtgttgtctc
     2041 aaacttccag atacctgttt ccttacggtt ttcaacatcg atttctggca tgtttgacaa
     2101 ggccaattca ccttctgctt ggtcaaagcg aagagagagg tcaaaagttg ctttggcttc
     2161 aggctcatcc acacatggga aagcttcgcg cgcaaaatgg ctctcgaact gagtagacaa
     2221 gacctccttc ttgactccat caactgtata ataagaaggg taaatccctg tcatgttgtc
     2281 tgtaatttta ccagaaaagg caagaaccaa ttcaacttga ccagcctcag ccaattcgat
     2341 atgaagggct tcattgtcat ggtcaactgt aaatggacga gcttgacctg caacttctac
     2401 agaggtgatt tccaagtctt tttggtggag ggagatgcgg tcactctgtg cttgaccagt
     2461 gatggtcact ttcccagaaa aagtcttggt ctcacgactc aaatctaaaa ataaatcata
     2521 atgttcagga acaaattgct taataaaatg ttcaactgct tgcatagttt tctcctattc
     2581 taagtttaag agctgctgct cttcttcaaa caaacttatt atatcatatt ttgcttgaaa
     2641 aaaaaggatt ggacagcgat ttccaaaact ttcttccttc taatagtcta cagtgggtag
     2701 accttgcgaa attcttctaa aacaaccttg ctttcaggtg taaaagtcag ccctacttca
     2761 cccaaacagt ctgtgaaaaa gtcctcatga acctggcgcc aataggcaag tgatttgtct
     2821 ccctcacctt ccttaaaggc atgttgcgca gaaacttgat tgaagagttc aacagaaacc
     2881 tttgtaattt cgacaatgca gacagactga ttttgactat ctaaaatgat atcgaaggtc
     2941 cctacttggg gaagtggttc gtcttctagt acatagaggt cataggctga tgctgttgct
     3001 gtcttttctc ctttaaacac caaatccgct aaaagatctg gttcaactcc aaaagcccaa
     3061 gcatcgattt catctccgat caaaggattg atttgcttgt atttattcca catttcttgc
     3121 ggtatcatgg gtactccttt gtaatttttt actttcttct tttatgtgtt taagatgatc
     3181 tggatggtca atctctaaat caaaaatctc tggaatagaa ctgtagtgga taatgcactt
     3241 gatacccaac tgattcattt tttgtatgaa agaagtattc agatagcctg ctacagcaaa
     3301 atcaatcttg ttctttcttg ctttatcctg catatctctt agcatatcta acattattgg
     3361 actttccata tcatgccatt gactgtttct catagtcgca aaaacaaagg aagtcaaatc
     3421 attcattcca actacaatct ttgaaatgcc cgtttccagt atactagata agtcaaaata
     3481 cgctgacggt aattcaatca tcgttccgac tttcccagta aaaccctgct gacgcaatac
     3541 tgtaatagct tgttttaatt ggtcggcatc attgacaaaa ggaaagataa gagatagatt
     3601 gggattggtt tgataaactt ctgtaacgac atgtgcttca gcctgaaatt catccaaaca
     3661 cgccagtaaa cgcctggttc ctctatagcc aaacaaggga tgcccttcgt caaaaaactc
     3721 tctagtcccc actaaacaat tggcttctgt attcgttaat tcagaaaaac gataccaaac
     3781 ttcttcatct gagtacaggg agcagatagt ctctagataa tcttttacaa attgctgaca
     3841 acttgctaat atgatgtttt gattaagctc tctcaacaaa tattccccac gaatcatgcc
     3901 gacgtggtga aatagttgag gataaatttt ttcaagaatt ttttcgccac taagggcaag
     3961 ttgatttctc atagccattc acctcgtctt aaaa
//
LOCUS       DAVLVF010000113         3976 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVLVF010000113 DAVLVF010000000
VERSION     DAVLVF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.113"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            92..1483
                     /locus_tag="VYN66_001856"
     CDS             92..1483
                     /locus_tag="VYN66_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1495835.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            1626..2543
                     /gene="adcAII"
                     /locus_tag="VYN66_001857"
     CDS             1626..2543
                     /gene="adcAII"
                     /locus_tag="VYN66_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV1495836.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            2551..>3976
                     /gene="phtD"
                     /locus_tag="VYN66_001858"
     CDS             2551..>3976
                     /gene="phtD"
                     /locus_tag="VYN66_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEV1495837.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANPVQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVRDSRPEQPSPQSTPEPSPSPQPAPNPQPAPSNPIDEKLVKEAVRK
                     VGDGYVFEKNGVSRYIPAKNLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFY
                     NKAYDLLARIHQDLLDNKGRQVDFE"
BASE COUNT         1202 a          743 c          836 g         1195 t
ORIGIN      
        1 tcagattttt ttagaaaaac attttataaa tattcacata tctcctatat ttatggtaaa
       61 atagaatcat cagtttattt tggagtcaaa gatgaatata tttagaacaa agaatgttag
      121 tttagataaa acagagatgc ataggcattt gaagttatgg gatttgattt tgctgggtat
      181 cggagccatg gtagggacag gcgtctttac aatcacaggt actgcagctg caacacttgc
      241 tggcccagcc ctagtgattt caatcgttat ttctgccttg tgtgtgggat tatcagccct
      301 cttttttgca gaatttgcct cgcgagtacc cgctacagga ggtgcctata gttacctcta
      361 tgctatctta ggagaattcc ctgcctggtt ggctggttgg ttaaccatga tggagttcat
      421 gacagccata tcaggcgtag cttcgggttg ggcagcttat tttaaaggct tgctctctca
      481 atacgggata gcctttcctc aggctttaaa tggtaccttt aatcctcaag cagggacatt
      541 tgttgattta ttgcctattc ttgtcttggt cttggtgact tcgcttgttt tactaaatgc
      601 caaggcagcc ttacgcttta attcgattct agttattttg aaattttccg ctttagctct
      661 ctttgtttta gtaggaattt ggaatattaa gtttgataat tggagcaatt ttgctcctta
      721 cggttttgga caaatctatg gagctagcac tggtattatg gctggtgcgt ccttgatgtt
      781 cttcggtttt ttgggatttg aatccatctc tatggctgta gatgaagtca agactcctca
      841 aaaaaatatt cctagaggga ttgtcttatc gctttctatc gtaaccattc tctatgcctt
      901 ggtgaccctt gttttgactg gtgtggttca ctatagtcat ttaaatgtcg atgatgccgt
      961 tgcctttgcc cttcgtagtg ttgggattag ttgggcagcc aactatgtgt cattagtggc
     1021 tatcttgacc ttgattacag tttgtatttc gatgacctat gccctatcgc gtatgattta
     1081 cagtttagca cgtgacggct tagtgcctgc tgcctttaag gaactgacga agactagcaa
     1141 gataccaaag aatgctacta tcttgacagg tctagcttca gcagtagcag caggaatgtt
     1201 cccactagcc agtattgcag cctttttaaa tatttgtacc ttagcctact tgatcatgtt
     1261 ggcttatggt ctgattcgct tacggaaaga aaaaggaatg cccaaagctg gggaatttaa
     1321 aacaccattg gtacctctgt taccaatttt atcgatcatc atttgtttat ccttcatgtt
     1381 acagtataat atgaatacct ggattgcttt cctagttgcg ttgttagtag gaagtattat
     1441 ttacttcact tatggctata agcattctac catagaagaa taaagtgaaa gtctattgag
     1501 ttgttgacac tcagcagatt tttatatgtg aaagaaattt caaatttttt ctaaaaatat
     1561 cttgacagat taaattttca ggagtagaat atttactagt taattaaatg gttaaggagt
     1621 tgttcatgaa gaaacaaaat ttatttttag tcctgttaag tgtctttctt ttgtgcttgg
     1681 gggcttgtgg tcaaaaggaa agtcagacag gaaaggggat gaaaattgtg accagttttt
     1741 atcctatcta cgctatggtt aaggaagtat ctggtgactt gaatgatgtt cggatgattc
     1801 agtcaagtag tggtattcac tcctttgaac cttcggcaaa tgatatcgca gccatctatg
     1861 atgcagatgt ctttgtttac cattctcata cactcgaatc ttgggcagga agtctggatc
     1921 caaatctaaa aaaatccaaa gtgaaggtct tagaggcttc tgagggaatg accttggaac
     1981 gtgtccctgg actagaggat gtggaagcag gggatggagt tgatgaaaaa acgctctatg
     2041 accctcacac atggctagat cctgaaaaag ctggagaaga agcccaaatt atcgctgata
     2101 aactttcaga ggtggatagt gagcataaag agacttatca aaaaaatgcg caagccttta
     2161 tcaaaaaagc tcaggaattg actaagaaat tccaaccaaa atttgaaaaa gcgactcaga
     2221 aaacatttgt aacacaacat acagcctttt cttatctagc gaagagattt gggcttaatc
     2281 aacttggtat tgcaggtatc tctcctgaac aagaaccaag tccacgacaa ctaacagaaa
     2341 ttcaggaatt tgttaagacc tataaggtta aaacgatttt tacagaaagt aacgcttctt
     2401 caaaagtagc tgaaactctt gtcaaatcaa caggtgtggg tcttaaaact ctgaatcctt
     2461 tagagtcaga cccacaaaat gacaagacct atttagaaaa tcttgaagaa aatatgagta
     2521 ttctagcaga agaattaaag tgaggaaaga atgaaaatca ataaaaaata tctagcaggt
     2581 tcagtggcag tccttgccct aagtgtttgt tcctatgaac ttggtcgtca ccaagctggt
     2641 caggttaaga aagagtctaa tcgagtttct tatatagatg gtgatcaggc tggtcaaaag
     2701 gcagaaaatt tgacaccaga tgaagtcagt aagagagagg ggatcaacgc cgaacaaatt
     2761 gttatcaaga ttacggatca aggttatgtg acctctcatg gagaccatta tcattactat
     2821 aatggcaagg ttccttatga tgccatcatc agtgaagaac ttctcatgaa agatccgaat
     2881 tatcagttga aggattcaga cattgtcaat gaaatcaagg gtggctatgt gattaaggta
     2941 gacggaaaat actatgttta ccttaaagat gcggcccatg cggacaatat tcggacaaaa
     3001 gaagagatta aacgtcagaa gcaggaacgc agtcataatc ataactcaag agcagataat
     3061 gctgttgctg cagccagagc ccagggacgc tacaccacag atgatggtta tatctttaat
     3121 gcttctgata tcattgagga cacgggtgat gcttatatcg ttcctcacgg cgaccattac
     3181 cattacattc ctaagaatga gttatcagct agcgagttag ctgctgcaga agcctattgg
     3241 aatgggaagc agggatctcg tccttcttca agttctagtt ataatgcaaa tccagttcaa
     3301 ccaagattgt cagagaacca caatctgact gtcactccaa cttatcatca aaatcaaggg
     3361 gaaaacattt caagcctttt acgtgaattg tatgctaaac ccttatcaga acgccatgtg
     3421 gaatctgatg gccttatttt cgacccagcg caaatcacaa gtcgaaccgc cagaggtgta
     3481 gctgtccctc atggtaacca ttaccacttt atcccttatg aacaaatgtc tgaattggaa
     3541 aaacgaattg ctcgtattat tccccttcgt tatcgttcaa accattgggt acgagattca
     3601 agaccagaac aaccaagtcc acaatcgact ccagaaccta gtccaagtcc gcaacctgca
     3661 ccaaatcctc aaccagctcc aagcaatcca attgatgaga aattggtcaa agaagctgtt
     3721 cgaaaagtag gcgatggtta tgtctttgag aagaatggag tttctcgtta tatcccagcc
     3781 aagaatcttt cagcagaaac agcagcaggc attgatagca aactggccaa gcaggaaagt
     3841 ttatctcata agctaggagc taagaaaact gacctcccat ctagtgatag agaattttac
     3901 aataaggctt atgacttact agcaagaatt caccaagatt tacttgataa taaaggtcga
     3961 caagttgatt ttgagg
//
LOCUS       DAVLVF010000114         3945 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVLVF010000114 DAVLVF010000000
VERSION     DAVLVF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.114"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..667
                     /locus_tag="VYN66_001859"
                     /pseudo
     CDS             1..667
                     /locus_tag="VYN66_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213952.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1499..1858
                     /locus_tag="VYN66_001860"
     CDS             1499..1858
                     /locus_tag="VYN66_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495838.1"
                     /translation="MGQEIKLIRKQFRITRQEEKQIKEMMREQKVDSFSEFLRQNLLK
                     KNYQDRIFESWFSLWQSQKFEQISRDVYEVLVVARENHQVTQEHVSILLTCVQELIAE
                     VNQVQPLSREFREKYMG"
     gene            1868..2197
                     /locus_tag="VYN66_001861"
     CDS             1868..2197
                     /locus_tag="VYN66_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV1495839.1"
                     /translation="MVYRYRTNLKKVFLTDPELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSHLISQDQLQELSKGVGELIKE
                     VDKEFQV"
     gene            2220..3945
                     /locus_tag="VYN66_001862"
                     /pseudo
     CDS             2220..3945
                     /locus_tag="VYN66_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050775.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
BASE COUNT         1456 a          579 c          772 g         1138 t
ORIGIN      
        1 aacaagaatc atgggttaat caatggttaa gtgaaaatat tataagtgag aaagatggaa
       61 gttatcaaca ttcactgact ataatgaagg atttcaataa tcaaaaaata gttaactata
      121 ttgctcattt caatagttat gacattttga aaaaaggttc tcacttatca ggtgcatata
      181 aacgaaaaat tttttctaaa gaaaatacac gattttggag tttaatatta actcgaaaga
      241 attttcgcaa caacttgata ttctctataa agaagttggt gtattaagtt tgggacaaaa
      301 agttgtagcc atgcttgatt ttttattagc atatagtgat tattctaaag acttcagacc
      361 attgattatt gatcagcctg aagacaatct agacaatcgt tatatttaca ggcatttagt
      421 tcagcagttt agagatgtga aagctcaacg tcaaattatt ttagcaacac ataatgctac
      481 aattgtaaca aattctatga cagatcaagt tgttattatg gagtcagatg gagttaacgg
      541 atggattgaa tcacagggat atgttagtga aaaatatata aaaaatcata tcatcaatca
      601 attagaggga ggaaaagatt ccttcaagca taaaatgtct atatatgaga cggctttatc
      661 agagtagagt cagaaaaagt aggttagaaa tttagcctac ttttttcttt gtccgacagg
      721 catagtgtac atctgaggtc caagtcctct gtggatattt gctgcagatg aaaccaatag
      781 cgactcctaa gcctgaatat cgtgaggtag gggggatagg aaggaattag cgaaatcaag
      841 gttctacaaa cagaatcgtg acttgaaacc atatatagcg gatgaggaac tctaaaatcc
      901 aaataggtgt cgtaacctat atacgtaaat tacgagagta aactaggaaa gatgtacggc
      961 ttattccgtg agcgtttagg acgtagtaca acgaatcatg ggagtcagct gaacacatag
     1021 tattgaagaa atttctgtaa tggaaatgga gcgaagaagt gaacaattaa atgaatacct
     1081 ctctaattaa atttgtcaat tctaattcct ggtatgaaaa gacagtgacc tgaaaatgta
     1141 aacgatggga gctgatcata aatataggac ggtacatgca gtggtgttag agattagtcc
     1201 ttacttgatt tgtgataact tccccaaatt tcttctgcta tacttttctc aacttttaaa
     1261 aatccaacta agaattttac ctgggggttt gggggcggag ccactaagtt atcttatcgt
     1321 tagctgtcaa aactggtagg ttttgatagg ctggcgatat gatttttggg atattgtgga
     1381 cacaatatct gagctcgcaa agaccgaaca aggagagact gaggtcttta ggaatcgtac
     1441 tgacaatgtg aggcagcctt acattgtcag acaagtataa tgttgaaaga atggtaagat
     1501 gggacaagaa attaagttaa tccgtaagca atttagaatc acgagacaag aagaaaaaca
     1561 gataaaagaa atgatgaggg aacaaaaagt ggatagtttc tcagaatttc ttcgtcaaaa
     1621 tttattgaaa aagaattatc aggatagaat ttttgaaagt tggttctccc tttggcagtc
     1681 tcaaaagttt gaacagatta gtcgagatgt gtatgaagtt ctggttgtcg caagagaaaa
     1741 tcatcaagtg actcaagagc acgtctcaat cttattgact tgcgttcaag aattgattgc
     1801 agaagtgaat caagtacagc cactcagtcg tgagtttcgt gaaaaatata tgggttagga
     1861 ggatgtaatg gtttatcgct atcgtaccaa tctcaaaaaa gtatttctaa ctgatccaga
     1921 attacaccaa ttgaatgaac ggattgctaa gagcaattgt caaaattttt cagtctatgc
     1981 cagaaaagtt ttgcttaatc ccaatatgtc atttgtcacg attaacactg atacttatga
     2041 ccagttagtt tttgaattga gacgtattgg aaataacatc aatcaaattg cgcgtgcgat
     2101 taatcaaagc catctgattt ctcaggacca attacaagaa ttgagtaaag gagtcggaga
     2161 gttaattaag gaagtggaca aagaatttca agtgtaggtg aaaagactga aggagtttca
     2221 tggtagttac taaacacttt gcgacgcacg gaaaaaaata tcgtaggcgt ttgattaaat
     2281 atatcctcaa tccagataaa acagataatc taaaattagt atctgatttt ggcatgagta
     2341 attacttaga ttttcctagc catacagaaa tggtagaaat gtacaatgtt aactttacta
     2401 ataacgacaa gttgtacgag tccagaaatg accgacaaga aaaacaccaa caaactattc
     2461 atgcccatca tctcatccaa tcattttctc ctgaagataa tctgacacct gaagaaatta
     2521 accgcattgg ttatgagacc atgatggaat tgacaggagg tcgttttaag tttatcgtag
     2581 caactcacac agacaaagat catgttcata atcacatcct aatcaacgct attgaccgca
     2641 attcagataa aaagttgata tggaactatg ccttggaacg aaacctccgt atgatttcag
     2701 accgtatttc taaaatggct ggtgcaaaaa ttattgagaa acgttattct tatcgaggtt
     2761 ataaaaaata tagggaatct agtcataaat ttgaattgaa acaacgtctt tattttttga
     2821 tgcagcaatc taaatagttt gatgattttt tagagaaagc aaagcagttg catgttcaga
     2881 ttgattttag tcaaaagcat agtcgattca tgatgacgga tcgagcaatg acaaaaccta
     2941 ttcgaggacg ccaactcagc aaacgagatc tatatgatga agaatttttt agaacgcatt
     3001 ttgctaagca agagattgaa agtcgtttag aatttttgtt gaaccgtgtc aattctttag
     3061 aagagttaat aacaaaagca aaagaattga atctaaccat tgacttaaaa caaaaaaatg
     3121 taacttttat cctgaaagaa aataatcaaa agataagttt gggtcatcaa aaaataagtg
     3181 ataagaaatt atatgatgtc aaattttttc aagattattt taaaaataag gaagtcattg
     3241 cttcagaagg attagagaat ttacaggaac agtaccatgc tttccaagaa gaacgagata
     3301 aggataaagt atccactgaa gagattgagg aagcctttaa gacatttaag aagcctttaa
     3361 gacatttaag gaaaaatgag ataccgttcg tgaatttgaa gtggaacttg cagagaacca
     3421 aatagagaag ctagttgata agggcgtcta tatcaaggtg tctttcggtg tcaaatagag
     3481 cggtcttatt ttcattccca actatcaatt ggatattatg gaagaagaaa atcataaaaa
     3541 atataaagtc tacattcgag aaacgtcttc ttactttgtc tataataaag aaaacatgga
     3601 taataattgt ttcatcaagg gtaggacctt aatcagacaa ttgagcaacg atagccaaaa
     3661 actcccctat agacgaccaa ctttaaaaag tcttcaagag aagatttctg aaattaatct
     3721 tatgattgaa ctatctaaca cgaacaaaca ataccaagaa atcaaagatg agcttgtatt
     3781 agaaattgca gagattgata tgaagctaga agaaacccaa gaaaaaatcg ccaccttaaa
     3841 caagatggcg gaagtcctta tcaatttgaa gagtgaagat gagatcggta gaaaattagc
     3901 taagtatgat tttgaccaaa tgaacatgac ggaatcaata atgtt
//
LOCUS       DAVLVF010000115         3936 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVLVF010000115 DAVLVF010000000
VERSION     DAVLVF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.115"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..992
                     /locus_tag="VYN66_001863"
     CDS             <1..992
                     /locus_tag="VYN66_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1495840.1"
                     /translation="NLKNSKKILLLTVTKNMEKVLDKLLESDELSWKLVAVSVLDKSD
                     FQHDKIPVIEKEKIIEFATHEVVDEVFVNLPGESYDIGEIISRFETMGIDVTVNLKAF
                     DKNLGRNKQIHEMVGLNVVTFSTNFYKTSHVISKRILDICGATIGLILFAIASLVLVP
                     LIRKDGGSAIFAQTRIGKNGRHFTFYKFRSMRIDAEAIKEQLMDQNTMQGGMFKIDND
                     PRVTKIGRFIRKTSLDELPQFWNVFIGDMSLVGTRPPTVDEYDQYTPEQKRRLSFKPG
                     ITGLWQVSGRSKITDFDDVVKLDVAYIDNWTIWKDIEILLKTVKVVFMRDGAK"
     gene            998..1741
                     /locus_tag="VYN66_001864"
     CDS             998..1741
                     /locus_tag="VYN66_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEV1495841.1"
                     /translation="MNERMQILGVTIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCHEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMQCLLELSNKKG
                     YSVYFFGAKEEILQDMLKVFKQRYPNLNVVGYRNGYFSPEDEKKIQEDIKGGKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQKLNLEWLFRVVN
                     EPKRLFKRYFIGNVTFIKRVLDEKRKSKK"
     gene            1716..2801
                     /locus_tag="VYN66_001865"
     CDS             1716..2801
                     /locus_tag="VYN66_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1495842.1"
                     /translation="MKSENQKNNILHISRTMDIGGAERIVYQLSSDLKDEFDSVHVAS
                     TGGLWESKLLSKGVQHHKILDVDSKNPLTMVKILSSFYRIIKNNDITIVHTHHRMAAF
                     YIRLLKIINPKLIHVYTAHNVFKNKLPLYKFSLKKAYCVAVSKAVNDNLKHDVGISDS
                     TVIYNGVVFEKTDKLVGEIVNYNGIKLGCIARLSEQKGLTYLIDAMSLVKHRNITLFI
                     VGDGELRDELENKVKQLNLQKSVIFLGYRKDIVECINSFDYLVSSSLYEGFGLNAIEA
                     FMKGKTMVSSDIPGINEVVNNKNGILVPVKDDVALARAIEKLATDKKLREKLAYQAKK
                     DYETKFNYSLFLDNYRRLYRKLMGESK"
     gene            2798..3886
                     /gene="wecB"
                     /locus_tag="VYN66_001866"
     CDS             2798..3886
                     /gene="wecB"
                     /locus_tag="VYN66_001866"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEV1495843.1"
                     /translation="MKKVMLVFGTRPEAIKMCPLVNELKQNDSIKTLVCVTGQHKEML
                     EQVLEVFKVVPDYDLGIMKANQTLFTITTSILDKIQAVLEKEKPDLVLVHGDTTTTFA
                     TALAAFYMGIKVGHVEAGLRTYNLQSPFPEEFNRQTTSIIADYHFAPTEVAKENLLRE
                     DRKNIFVTGNTVIDALKTTVQDNYHHPILEWAKGSKLIMLTAHRRENLGKPMENMFNA
                     VNRILNEFEDVKVVYPIHKNPKVRELASKVFGDNERMKIIEPLEVIDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVVAGTLKLVGTEEENIYSNFKLLLEDET
                     EYNKMSKASNPYGDGMTCKRIVDIILEV"
BASE COUNT         1371 a          522 c          823 g         1220 t
ORIGIN      
        1 ttaatctaaa aaatagcaag aaaattttac tgttaacagt aacgaaaaat atggaaaaag
       61 ttcttgataa attgctagaa tctgatgaac tttcatggaa attggtagca gtaagtgttt
      121 tggataaatc tgattttcaa catgataaaa tacctgtaat tgaaaaggaa aaaattattg
      181 aatttgcaac gcatgaagtt gtggatgagg tgtttgtcaa tcttccagga gagagctacg
      241 atattggaga aattatctct aggtttgaga caatggggat agatgtaact gtaaatctta
      301 aagcatttga taagaatttg ggtcgcaata aacaaattca tgagatggta ggattgaatg
      361 tagtcacttt ctctacaaat ttttataaaa ctagtcatgt gatttcaaag agaattctcg
      421 atatttgtgg tgccactatt ggccttattc tttttgctat agctagtcta gttttagttc
      481 cattgattcg taaagatggc ggatcagcta tttttgctca aactcgtata gggaaaaatg
      541 gtcgacattt taccttttat aaattccgtt cgatgcgaat cgatgctgaa gctatcaaag
      601 aacagttgat ggatcaaaat acgatgcaag gtggtatgtt taagatagac aatgatcctc
      661 gtgttacaaa aattggtcgc tttattcgta aaactagttt agatgaattg ccgcagtttt
      721 ggaatgtctt tataggagat atgagtttgg tgggaacacg tccacctaca gtagacgagt
      781 atgatcagta tactccagaa cagaaacgtc gactcagctt taaacctggt attacaggtt
      841 tatggcaggt tagcggccgt agtaaaataa ccgattttga cgatgttgta aaattagatg
      901 tggcttatat tgataattgg acaatctgga aagatattga aattttgctt aaaactgtta
      961 aagttgtatt tatgagagat ggagcgaagt aattcatatg aatgagagaa tgcaaatatt
     1021 aggagttact atagatccat taacgatgaa agaaacagtg gatgctgttg aacagtatgt
     1081 tttaaagaag catcctttgc acttgatggg agttaacgca gataaaatta atcagtgcca
     1141 tgaagatgag aaaatcaaaa aaatcgttaa tgaatcagga atcattaatg cggatggagc
     1201 atcagttgtt cttgcaagta agtttttagg aacgcctgtt cctgaacgag tagcgggtat
     1261 tgatttgatg caatgtcttt tagagttgtc aaataaaaaa ggatattcag tttacttttt
     1321 tggagctaaa gaagaaattt tacaagatat gctaaaagtt tttaaacaaa gatatcctaa
     1381 tcttaatgtt gttggctata gaaacggata tttttcacct gaagatgaaa aaaagattca
     1441 ggaagatatt aaaggaggaa aaccagattt tgtatttgta gggattactt ctcctaaaaa
     1501 ggagtatatc atccaatcat tcatggataa tgggattaat gctgtcttta tgggtgttgg
     1561 tggcagtttt gatgtattat caggacacat taaacgagct ccattatgga tgcaaaaatt
     1621 gaatctggaa tggttgttcc gtgtagttaa tgaacccaaa cgccttttta agcgttattt
     1681 tatagggaat gttactttta ttaagcgagt tttagatgaa aagcgaaaat caaaaaaata
     1741 atattctaca tatttctcgt acaatggata ttggtggtgc agaacgtatt gtatatcaat
     1801 tgagttctga tttgaaagat gaatttgata gtgtccatgt cgcttcaaca ggtggactat
     1861 gggagagcaa acttttatca aaaggagttc aacatcataa aattttagat gttgacagta
     1921 agaatcccct aactatggta aagatccttt caagttttta tagaattatc aaaaataatg
     1981 atattaccat tgttcacact catcatcgga tggctgcctt ttatattcgc ttgttgaaaa
     2041 tcattaatcc taagttgata catgtttaca ctgcacataa tgtatttaaa aataaattac
     2101 cattatacaa attttcctta aaaaaagcgt attgtgtagc tgtaagtaaa gcggttaatg
     2161 ataatttgaa acatgatgta ggaatcagtg atagtacagt aatctataat ggggttgttt
     2221 ttgaaaaaac tgataagcta gttggtgaaa ttgtaaacta taacggaatc aaattggggt
     2281 gtattgctag gttatcagag caaaaaggat tgacctacct aattgatgcg atgtctttag
     2341 tgaaacacag gaatattacg ttgttcattg tcggagatgg agagcttcga gatgagcttg
     2401 aaaacaaggt gaaacaatta aatcttcaaa aatcagtgat atttttaggt tatcgaaaag
     2461 atattgttga atgtatcaat agttttgatt acttagtgtc atcatcttta tatgaggggt
     2521 ttggtctaaa tgcaattgaa gcatttatga aggggaaaac gatggtttca tcagatatcc
     2581 caggaataaa tgaagtagtc aataacaaaa atgggattct tgttcctgtt aaggatgatg
     2641 ttgccttagc gagagcgatt gaaaagcttg cgactgacaa aaaacttaga gaaaaactcg
     2701 cttatcaagc aaaaaaagat tatgagacaa aatttaatta ttccttgttt ttggataatt
     2761 atcggagact ttatagaaaa ttaatgggag aatcaaaatg aaaaaagtca tgttggtttt
     2821 tgggacacgt ccagaagcta ttaaaatgtg tcctttagta aatgaactga aacaaaatga
     2881 ttcaatcaag acacttgtgt gtgtaactgg tcaacataag gaaatgttag agcaggtttt
     2941 agaggtgttc aaggttgtcc cagattatga tttgggtatc atgaaagcaa atcagaccct
     3001 atttaccatt acgactagta tcttggataa aattcaggcg gttctagaga aagaaaaacc
     3061 agatcttgtt cttgttcatg gagatactac aacaacattt gcgacagctt tagcagcatt
     3121 ttatatggga atcaaagtgg gacacgtcga agcaggtctc agaacctata atctccaaag
     3181 tccatttcca gaggaattca atcgccagac aacttctatc attgccgact atcattttgc
     3241 gccgactgaa gttgcgaagg aaaatctact aagagaggac agaaaaaata tctttgtgac
     3301 gggaaataca gtcattgatg ctttgaagac gactgttcaa gataactacc atcacccaat
     3361 tttagaatgg gcaaagggta gtaagttaat catgctaacg gctcatcgac gagaaaatct
     3421 tgggaaaccg atggaaaaca tgtttaatgc tgttaatcga attttaaacg aattcgaaga
     3481 tgtaaaagtt gtatatccca tccataaaaa tcctaaggtt cgagaattag caagtaaagt
     3541 ttttggcgat aatgaacgaa tgaaaatcat tgaaccgtta gaggttattg attttcataa
     3601 ttttatgaat caaagctata tgattttgac agattctgga ggtgtacagg aagaagcacc
     3661 ttctttagga aaacctgtct tggtgatgcg tgatacaaca gagcgtcctg agggggtcgt
     3721 tgctggcacg ttgaagttgg taggaacaga agaagagaat atctatagca acttcaaact
     3781 tcttcttgaa gatgaaacag aatataacaa gatgagcaaa gctagcaacc cttatggaga
     3841 tggaatgact tgtaaacgga ttgttgatat tattctggaa gtataaaaat aattcaatta
     3901 tggatgagga aagaataaat tagattgatg ataaaa
//
LOCUS       DAVLVF010000116         3836 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVLVF010000116 DAVLVF010000000
VERSION     DAVLVF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.116"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(157..912)
                     /locus_tag="VYN66_001867"
     CDS             complement(157..912)
                     /locus_tag="VYN66_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEV1495844.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCFSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPCFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(982..3633)
                     /locus_tag="VYN66_001868"
     CDS             complement(982..3633)
                     /locus_tag="VYN66_001868"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV1495845.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            complement(3645..>3836)
                     /locus_tag="VYN66_001869"
     CDS             complement(3645..>3836)
                     /locus_tag="VYN66_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV1495846.1"
                     /translation="IKSKLESLKLKHSRYYISKISKYDLKYNPGNYTMSPLAAFAEIE
                     EIYIELSKIGLVKIVNKSN"
BASE COUNT         1076 a          854 c          758 g         1148 t
ORIGIN      
        1 aaataataaa agagttataa tcagcactac tgataaattc aataccgatt tcgccatctt
       61 aaaacttagt caaacgaata ccgttgattt ccagttcatt aagtcgttga cctgtcaaga
      121 cttctcgttc cttcaactgg aaaagttaag ggggaactaa tacaaatcct ctacgcttct
      181 atattcaatg atttcatttt tgataatatt ctttttaaac tcaaaatgtt ttaaaggatt
      241 atcgattaac actaactttg gaatatcatc tagatttgtg accaaggata aaataaaatc
      301 cgattgaaac tccttgactt tttcaaattc ttttgcagtt aaacgcacac gccccttaga
      361 ttttcggata ccttttactt cggcaaggta agaatactct tggacatcta cctgaaagtc
      421 atatccatca ccataaagac gggcatcggt taactgtcca ccttggaatt tttcttcttt
      481 atcaaaatga aggataaaat aattctcagc ctccattccc gtttcctgta gacgtttgaa
      541 tttagttcga actattggtt tttcaacgat ggttatgcct gttttcttcc cttcactggc
      601 aagtaacatt ttaactattt cagcataact atgaacatct tcatttccaa acatcatatc
      661 aattaaatct ttacgaaaac gataaacttc agctttctgc caccaacctt ttcgattatt
      721 gtcaaaacaa ggatcaaata aatccattct attttttaca actccagttg tttcagcaat
      781 tcccagagaa accacatagc gataaaactc cgacttgcta aagcattgaa attcttttat
      841 aaaaagcttg tcaaacttgg caagaccgta gccaattaaa tttaacaatt cataatgagg
      901 gtgtttggac atcactttct ccatcaatag atattgattt cattatacca aaaaacacgg
      961 ctcatcaccg tgtttctgtg tttatttcac caacttcttc atctcatcaa tacgtgctac
     1021 ggtcacatca tacttggctt ggtagtcggc ttgtttgtct ttttcttttt ggacaacttc
     1081 tggtttagcg ttggcgacga agcgttcgtt agagagtttc ttaccaacca tgtccagttc
     1141 tttttgccat ttagcgagtt ctttgtcgag acgagctagt tcttcttcaa cattgaggag
     1201 atcggccagt ggcaagaaga tttcggctcc tgtaataacg cttgacatag ccagttcagg
     1261 tgcagggatg gttgatgcga tttccaagtg ttctggattt gtaaagcgtt tgatatagtt
     1321 gacattgctg ttaaagaagg cttccaagtc actatcgctt gtcttaacaa ggatggtgat
     1381 aggcttgctt ggtgctacat ttacttccgc acgcgcattc cgaacagcac gaattaagtc
     1441 tttgagactt tccacaccag tgtgagccgc aaggtcttca aaggctagat taacagttgg
     1501 gtatgcagct gtcacgatag aaccttctga gatttgtcca aagatttcct ctgtcacgaa
     1561 tggcatgatt gggtgaagga gacgaaggat cttgtccaaa gtgtatagta gaacagaacg
     1621 tgtgatgact ttctcttctt cgttatcgct ataaaggact tccttggtca actcaacata
     1681 ccagttggca aattcttccc aaatgaagtt gtaaaggata tgaccagcca caccaaactc
     1741 gaacttatca aagttttcag taacttttgc aatggtttcg ttgagattgt ggagaatcca
     1801 gcggtccgtc acattaccag cctcacctgt tgcaactttt gtgacattgt catgcgccac
     1861 atccagcgtc aaacctccat tgttcatgag gatatagcga gaaatgttcc aaatcttgtt
     1921 aataaagttc catgaagcat ccattttctc ataagagaaa cgcacgtctt gtcctggcgc
     1981 agaaccgttt gaaaggaacc atcgaagggc atcggcaccg tatttctcga taacatccat
     2041 tgggtcaatc ccgttaccga gagacttaga catcttgcgt ccttgctcgt cacgaatgag
     2101 accgtggata aggacgtttt ggaatggttg acggcctgtg aattccaatg actggaagat
     2161 catacgagac acccagaaga agatgatgtc gtaacctgtt accaaggttg aagttgggaa
     2221 ataacgttta aagtcttctg agtcgacttc aggccagccc atggttgaaa atggccagag
     2281 ggcagaactg aaccaagtat ccaagacgtc ttcgtcctga gtccatccgt caccttctgg
     2341 agcttcttcg ccgacataca tttcaccatc agcattgtac caggcaggga tttggtgacc
     2401 ccaccaaagc tgacgagaga taacccagtc gtggacattt tccatccatt gaaggaaggt
     2461 atcgttgaaa cgaggtgggt agaattcgac cttgtcctct gtgtcttggt tggcaatggc
     2521 gttcttagcc aattggtcca tcttgacgaa ccattgagta gacaagcgtg gctcaactac
     2581 gacacctgtc cgttctgagt gaccgactga atggacacgt ttttcgattt tgacaagggc
     2641 accgatttct tccaacttag caacgactgc cttacgagct tcaaaacgat ccatgcctga
     2701 aaattcaaag gcaagctcat tcatagttcc gtcgtcgttc atgacgttga cttgtggcaa
     2761 gttatgacgt tggccaacca agaagtcatt tggatcgtgg gcaggtgtga ttttcacgac
     2821 accagtacca aactcaggat ctgcgtgctc atctccaacg attgggatga gtttattagc
     2881 gattggaagg atgacgtttt taccaatcaa gtccttgtag cgcgggtctt ctggattaac
     2941 cgcaaccgca acgtccccaa acatagtctc aggacgagtt gtagcaactt caagggcgcg
     3001 tgaaccatct tccagcatgt aattcatgtg gtagaaggca ccttctacat ccttgtgaat
     3061 cacctcaata tcagaaaggg ctgtgcgagc tgctgggtcc cagttgatga taaactcacc
     3121 acgatagatc cagcctttct tgtaaaggtc cacaaagacc ttacgaacag cttttgacaa
     3181 accttcgtca agagtgaaac gctcacgaga atagtctaca gaaagcccca tcttgcccca
     3241 ttgttccttg atggtagtgg catattcgtc tttccattcc cagaccttcg tcaagaaaga
     3301 ctcacgacct aggtcataac gcgtaatacc ctcaccacgt aagcgctcct caaccttagc
     3361 ctgagtcgca ataccagcgt ggtccatacc tggaagccaa agggtatcaa agccctgcat
     3421 gcgtttttga cggatgatga tatcctgcaa agtcgtatcc caagcgtgac caaggtgaag
     3481 tttcccagtt acgtttggtg gtggaatcac gattgaataa ggcttagcct tttgatcgcc
     3541 tgaaggcttg aaaacatccg catcaagcca tttttggtaa cgaccagcct caacctcggc
     3601 tggattgtat ttagatgaaa gttctttaga catgtgtgtt ctccttaatt acttttattg
     3661 acaattttaa ccaaaccaat ttttgaaagt tcaatataaa tctcttcaat ttcagcaaat
     3721 gctgcaagtg gtgacatagt ataatttcca ggattatatt taagatcata cttgcttatc
     3781 tttgaaatat aatacctaga gtgtttcaat tttaatgatt ccaactttga tttaat
//
LOCUS       DAVLVF010000117         3760 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVLVF010000117 DAVLVF010000000
VERSION     DAVLVF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.117"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            288..1667
                     /locus_tag="VYN66_001870"
     CDS             288..1667
                     /locus_tag="VYN66_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1495847.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            1681..2346
                     /locus_tag="VYN66_001871"
     CDS             1681..2346
                     /locus_tag="VYN66_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV1495848.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            2460..2555
                     /locus_tag="VYN66_001872"
                     /pseudo
     CDS             2460..2555
                     /locus_tag="VYN66_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2746..3285
                     /locus_tag="VYN66_001873"
     CDS             2746..3285
                     /locus_tag="VYN66_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1495849.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKGHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQEFESKGQEVDVFVTNFVYEKEGQSCKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YIMMHSLIYRTDLLRASQF"
     gene            3352..>3760
                     /locus_tag="VYN66_001874"
     CDS             3352..>3760
                     /locus_tag="VYN66_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1495850.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLY
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
BASE COUNT          997 a          712 c          865 g         1186 t
ORIGIN      
        1 tatccacaac ctcaaaacag tgttttgagc aacctgtgac tagctttcta atcgatgcct
       61 tggttttcat tgcctataat caaaaagaga aattttctcc tgaaaagcat atagagtagc
      121 tggcgttaaa agctcctgtc ttgctttttt gacctatagt catatctatc aagtattgtt
      181 cttgcctaag ctatcaataa aaaggtggca ttttttaggc ttggtgttag tgaattttgc
      241 cttatcctat ctaagtcatt tcgagctttt tatggtacaa tggaaacatg ttattcaaat
      301 tatctaagga aaaaatagag ctaggcttat ctcgtttatc gccagcccgt cgtatttttt
      361 tgagttttgc cttggtcatt ttactaggct ctcttctttt gagcttgccc tttgtccaag
      421 ttgaaagctc acgagcgact tattttgatc atcttttcac tgctgtctct gcagtctgtg
      481 tgacaggtct ctcaaccctt ccagtagctc acacctataa tatctggggc caaataatct
      541 gtttgctctt gattcagatc ggtggtctag ggctcatgac ctttattggg gttttctata
      601 tccagagcaa gcaaaagctt agtcttcgta gccgtgcaac tattcaggat agttttagtt
      661 atggagaaac tcgatctttg agaaagtttg tctattctat ttttctcacg acctttttgg
      721 ttgagagctt gggagctatt ttgcttagtt ttcgccttat tcctcaactt ggctggggac
      781 gtggtctttt tagttccatt tttctagcga tctcagcctt ctgtaatgcc ggttttgata
      841 atttagggag caccagttta tttgcttttc agaccgattt actggtcaat ctggtgattg
      901 caggcttgat tattacaggc ggccttggtt ttatggtctg gtttgatttg gctggtcatg
      961 taggaagaaa gaaaaaagga cgtctgcact ttcatacgaa gcttgtatta ttattgacta
     1021 taggtttgtt gttatttgga acggcaacta ctctctttct tgagtggaac aatgctggaa
     1081 cgattggcaa tctccctgtt gccgataagg ttttagttag cttttttcaa acagtgacga
     1141 tgcgaacagc tggcttttct acgatagatt atactcaggc tcatcctgtg actcttttga
     1201 tttatatctt acagatgttt ctaggtgggg cacctggagg aacagctggg ggactcaaga
     1261 ttacgacatt ttttgtcctc ttggtctttg cacgaagtga gcttctaggc ttgcctcatg
     1321 ccaatgttgc gagacgaacg atcgcgccgc gaacggttca aaaatccttt agtgtcttta
     1381 ttatcttttt gatgagcttc ttgataggat tgattctgct agggataaca gccaaaggca
     1441 atcctccctt tatccacctc atatttgaaa ccatttcagc tcttagtaca gttggtgtaa
     1501 cggcaaatct gactcctgac cttgggaaat tggctctcag tgttatcatg ccacttatgt
     1561 ttatgggacg aattggtccc ttgaccttgt ttgttagctt ggcagattac catccagaaa
     1621 agaaagatat gattcactat atgaaagcag atattagtat tggttaagaa aggaaagagc
     1681 atgtcagatc gtacgattgg aattttgggc ttgggaattt ttgggagcag tgtcctagct
     1741 gccctagcca agcaggatat gaatattatc gctattgatg accacgcaga gcgcatcaat
     1801 cagtttgagc cagttttggc gcgtggagtg attggtgaca tcacagatga agaattattg
     1861 agatcagcag ggattgatac ctgcgatacc gttgtagtcg cgacaggtga aaatctggag
     1921 tcgagtgtgc ttgcggttat gcactgtaag agtttggggg taccgactgt tattgctaag
     1981 gtcaaaagtc agaccgctaa gaaagtgcta gaaaagattg gagctgactc ggttatctcg
     2041 ccagagtatg aaatggggca gtctctagca cagaccattc ttttccataa tagtgttgat
     2101 gtctttcagt tggataaaaa tgtgtctatc gtggagatga aaattcctca gtcttgggca
     2161 ggtcaaagtc tgagtaaatt agacctccgt ggcaaataca atctgaatat tttgggtttc
     2221 cgagagcagg aaaattcccc attggatgtt gaatttggac cagatgacct cttgaaagca
     2281 gatacctata ttttggcagt catcaacaac cagtatttgg ataccctagt agcattgaat
     2341 tcgtaaagag ggatgacccc tcttttttga tgcttaagat ggcaaataga gacagaagcc
     2401 ccttgtcttc tagtaaaagt tcttcaaagg ctggacttta tggtaaaata gaaggaagtg
     2461 acaagagaga gtaatactca atgaaaatca aagatcaaac taggaaacta gctgcgggct
     2521 gctcaaaaca ctgttttgag gttgtggata gaactgacga agtcagctca aaacactgtt
     2581 ttgaggttgc agatagaact gacgaagtca gctcaaaaca ctgttttgag gttgcagata
     2641 gaactgacga agtcagtaac atctatacgg caaggcgacg ttgacgcggt ttgaagagat
     2701 tttcgaagag tataagaaaa aatcagtccc ctaaaggagc agattatgaa gttattgtct
     2761 atcgccattt ctagctataa tgcagcagcc tatcttcatt actgtgtgga gtcgctagtg
     2821 attggtggtg agcaagttgg gattttgatt atcaatgacg ggtctcagga tcagactcag
     2881 gaaatcgctg agtgtttagc tagcaagtat cctaatatcg ttagagccat ctatcaggaa
     2941 aataaaggcc atggcggtgc ggtcaatcgt ggcttggcag aggcttctgg gcgctatttt
     3001 aaagtagttg acagtgatga ctgggtggat cctcgtgcct acttgaaaat tcttgaaacc
     3061 ttgcaggaat ttgagagcaa gggtcaagag gtggatgtct ttgtgaccaa ttttgtctat
     3121 gaaaaggaag gtcagtcttg taagaagagt atgagttacg attcagtctt gcctgttcgg
     3181 cagatttttg gctgggacca ggtcggaaat ttctccaaag gccagtatat catgatgcac
     3241 tcgctgattt atcggacaga tttgttgcgt gctagccagt tctaattgcc tgaacatact
     3301 ttttatgtcg ataatctctt tgtctttacg ccccttcagc aggtcaagac catgtactat
     3361 ctgcctgtcg atttctatcg ttatttgatt gggcgtgagg accagtctgt caatgagcaa
     3421 gtgatgatta agtgcattga ccagcaactc aaggtcaatc gactcttggt agaccaactt
     3481 tatttgtccc aagtgagtca tcccaaaatg cgagaatatc tgctgaatca tattgaaatc
     3541 acgacggtga tttccagtac cctgctcaac cgatctggaa cagcggagca tctggcaaaa
     3601 aaacgccaat tgtggaccta tattcagcag aaaaatccag aagtctttca ggctattcgt
     3661 aagaccatgt tgagccgttt gaccaaacat tctgtcttgc cagatcgcaa actgtccaat
     3721 gtcgtctatc aaatcaccaa atctgtttat ggatttaatt
//
LOCUS       DAVLVF010000118         3758 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVLVF010000118 DAVLVF010000000
VERSION     DAVLVF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.118"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            24..638
                     /locus_tag="VYN66_001875"
     CDS             24..638
                     /locus_tag="VYN66_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495851.1"
                     /translation="MKLKKTCVFQNLNFSCKQGEIIGITGANGSGKSVLFKLIAGLYS
                     PSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLASIRGVVGNQEIN
                     DTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMNALDKSSVENMR
                     TLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(718..1579)
                     /locus_tag="VYN66_001876"
                     /pseudo
     CDS             complement(718..1579)
                     /locus_tag="VYN66_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(1591..2349)
                     /locus_tag="VYN66_001877"
     CDS             complement(1591..2349)
                     /locus_tag="VYN66_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1495852.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDIFLDGQSIVENKKNFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKTQGRDKKEVTEEALQLLDRVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            complement(2350..3027)
                     /locus_tag="VYN66_001878"
     CDS             complement(2350..3027)
                     /locus_tag="VYN66_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1495853.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            complement(2987..3655)
                     /locus_tag="VYN66_001879"
     CDS             complement(2987..3655)
                     /locus_tag="VYN66_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1495854.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LSQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGDSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
BASE COUNT         1151 a          687 c          828 g         1092 t
ORIGIN      
        1 taatcatatt aaaaaatatt gagttgaaat taaaaaaaac atgtgttttt caaaacctta
       61 attttagttg taaacagggg gaaattatag gaattactgg tgcgaatggc tcagggaaaa
      121 gtgtattgtt taaattaata gctggtttat atagtccgtc ttatggagaa gtgttaatca
      181 atggggaaaa tattgttcct gagagaaaaa ttccagctaa tttgggagct ttgattgaag
      241 aacctggttt tataaattat tatagtggct ttaagaattt acaatatttg gcaagcatac
      301 gaggagtagt tggtaatcag gaaatcaatg atacactgaa aatagttggt ctatatgagc
      361 aaaaagacca gaaagttaaa acttattcgc taggtatgag gaaaaagcta gggattgctc
      421 aagcaattat ggagaatccc tctattcttt tactagatga acctatgaat gccttggata
      481 aatcaagtgt agaaaatatg agaacattgt ttagaaagct ctctagtgaa aaagggacaa
      541 caattttgat tgctagtcat agtgaagagg atattcgtat cttatgtgat aaagtatatg
      601 caatagaaga taaagtatgt acactgtgtt cagattgaac atcttagata gactcaggat
      661 gttataggta aaatcttaaa aagggattgg agcttaaaat ccagtccctt ttaagagtta
      721 atggccacct tcaacaacca ggtcatctgc tttagcagcg tcaccgtagg ttgggtgaag
      781 tgttttttca taggccttgt ggaagaagtt ttccttgcct aatttttcaa tatctttatt
      841 gatgaagtct agcaattctt ggttgccttt ttgaactgct gccgcaatgg tatcgggatc
      901 accgagggaa gtaattccta cttcaaatcc tttattttca agcgcccaag ctagaacttc
      961 cgtattgtca gttgaaaagg catctccacg tccgtcaaga agagcttggt aagagtcact
     1021 gtattggtcg tatttttgga gtttgatttc tggatgattc ttttcaaaat aagtctcagc
     1081 agtcgttcct tttgtgacaa ttaaggtttt accttcaagt tgtttgacgt ctgtaatgag
     1141 accagtctta ggtgatacga cacccagaga aactttcata tatggaaggg caaaatcaac
     1201 ttgtttctta cgttcgtcag ttactgtaaa gttagcaaga gtaatatcta ccttgtttga
     1261 aatcaagtat tccgcacggt tggcagcatc gactgaaatg tatttaacct tgacaccaag
     1321 gtctttagct agttggttcc ctagttcaat atcgtagcct tggtaagaac catcattgtc
     1381 aacgtagcca aacggttttt tatctccaaa cacggcgatt cgcagttcac cgcctttttt
     1441 tgatttcatc gatagtgcga gctttggcag ttgtttttcc agaggatgaa ccagcgtttc
     1501 cacctgagct acaagcagtg ataaagataa gggcaaaggc aagtgctaaa acagttaaga
     1561 gtggtttgaa tagtttcata agaatctcct ttatagatat gagccaaatt ggctaaagtc
     1621 aaagacgttt aaaaattcct gggctcgttt ggtttgcgga ttggtaaaga aggcttgagc
     1681 tgttccttct tcagcgattt tcccttggtc gaggaagata atccggtcag caatggcttg
     1741 ggcaaactgc atttcgtggg ttactaaaat catggtacgg ccttcttggg ccaaatcatt
     1801 gataagttcc agcacctcac gcaccatttc tggatccagc gaagcagtca cctcgtcaaa
     1861 aaggatgatt tctggatgca ttaggagggc acggacaatt gcaacacgtt gcttctgtcc
     1921 accagataat tgacgggcaa agctatgttg tttatccagc aaaccgacac gatctagtaa
     1981 ttgcaaagct tcttccgtta cttctttctt gtcccttcct tgagttttga tagggcctag
     2041 gatgaggttt tgtaagacat ccagatgggg aaagagttca taactttgaa agaccatgcc
     2101 aatcttttgg cgaactaggt gaaaattttt tttattttca acgatagact gaccatccag
     2161 aaaaatatct ccaccttgaa tactttctaa gccgttgagg caacgaagga gggtactttt
     2221 cccacaacca gatggcccta ggatgacaac aacttcccct tttttgattt ctagagaaag
     2281 tccttggagg atgggattgt ctccgaagga tttttttagt tccttgattt ctaagatagt
     2341 ttcagacatt tagtttctcc aatgtttttc taagtgagtg gatagtttgg aaatagggta
     2401 gcaaactgcg aaatataaga ctagaatggt tccataaatc caaaatgaag cagttgggat
     2461 ggtcaggcga ttgctatcga tgatttgttg tccaactttg gtcacttcca caaccccaat
     2521 caaaacaact aatgaagtgg ttttaatcat ccgagtgaca agattgatag cctgcggtag
     2581 cagtcttctc aagacttgtg ggatgatgat gtggtagtaa agttgaacat tagtcaagcc
     2641 gagtgcctgt ccactttcaa actgatgttt agggagagaa gtgatagctc cacgtaccaa
     2701 gtctcccatt tcagctgttc cccagagggt aaaaacgata atagctgaag tctcacctga
     2761 gatattgata ttaaagtttc gagccaagcc aaagtaaacg atgaagagta gcaccagctg
     2821 gggcatgata cggataaatt ccagatacaa tcgtgttaaa aatcgtatga ttctagaatg
     2881 ggaggtcatg atgattccca tgactgttcc gaacatcatg gataagagga cagacaggat
     2941 ggatatccca atcgtaacgc ccaatccctg taagattctc aggagattat ttccttgaaa
     3001 gagtacttgg attcccgaat cctgcatggc ggagcctcct ttctatccag ctaaagacca
     3061 gtgagatggg tagcagcatg atcagataag caactaccaa catagctagc gcaatgtctg
     3121 tctcatagta gagaccaatc aaatccttgg cgacgtacat gaggtcggcc aaagccactg
     3181 ctgagaaaac agaggtttcc ttgataagga aaatgacatt ggcactaaag gaggggagtg
     3241 ccaccgctgt tgcttgcgga agaaccacat agtaaaagac ctgtagaggt gtcagaccaa
     3301 tagcgaggcc aatctcctgc tgggtttgac tgatggcttc cagcccgctt cggaaagatt
     3361 ctgccatata ggagtctcct aaaaagacaa gcccaagcgt tgcacagact tctgaagata
     3421 ggacaatccc gattcgggga agaccgaagt agagaaagaa gagttgaatc aaaaggggcg
     3481 tattacgtga caattcaatg taggctgtcg ctacttgcga caaaacaagg attcgataat
     3541 gtcggatgat actaacgatt aaaccgagca gaaaggatcc caaaattccc caaactgcaa
     3601 tatgcaaggt cagaaagaat gccttttgat atagtggtag atattgttca acaatggatc
     3661 aatccaaaaa tagaacctcc catctagaaa taatacagtt attgtagcac ttaaaatctt
     3721 ctttggataa tatctatttt ttattgccgt tataagga
//
LOCUS       DAVLVF010000119         3689 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVLVF010000119 DAVLVF010000000
VERSION     DAVLVF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.119"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            103..330
                     /locus_tag="VYN66_001880"
     CDS             103..330
                     /locus_tag="VYN66_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495855.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            618..3500
                     /gene="nanA"
                     /locus_tag="VYN66_001881"
     CDS             618..3500
                     /gene="nanA"
                     /locus_tag="VYN66_001881"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEV1495856.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGGSSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKISRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYGNYNDAPLKVKPGQWNSVTFTVEKPTPE
                     LPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGVIG
                     LEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQKIIGIAKGSIESM
                     HNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLVTLTTK
                     EDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1254 a          623 c          836 g          976 t
ORIGIN      
        1 tatctttatg tatatttaaa gatagattgc tgtaaaaata gtagtagcta tgcgaaataa
       61 cagatagaga gaagggattg aagcttagaa aaggggaata atatgatatt taaggcattc
      121 aagacaaaaa agcagagaaa aagacaagtt gaactacttt tgacagtttt tttcgacagt
      181 tttctgattg atttatttct tcacttattt gggattgtcc cctttaagct ggataagatt
      241 ctgattgtga gcttgattat atttcccatt atttctacaa gtatttatgc ttatgaaaag
      301 ctatttgaaa aagtgttcga taaggattga gcaggaagta tggtgtaaat agcataggct
      361 gatgtccatc atttgcttat aaagagatat tttagtttaa ttgcagcggt gtcctggtag
      421 ataaactaga ttggcaggag tctgattgga gaaaggagag gggaaatttg gcaccaattt
      481 gagatagttt gtttagttca tttttgtcat ttaaatgaac tgtagtaaaa gaaagttaat
      541 aaaagacaaa ctaagtgcat tttctggagt aaatgtctta tttcagaaat cgggatatag
      601 atatagagag gatcagtatg aatcggagtg ttcaagaacg taagtgtcgt tatagcatta
      661 ggaaactatc ggtaggagcg gtttctatga ttgtaggagc agtggtattt ggaacgtctc
      721 ctgttttagc tcaagaaggg gcaagtgagc aacctctggc aaatgaaact caactttcgg
      781 gggggagctc aaccctaact gatacagaaa agagccagcc ttcttcagag actgaacttt
      841 ctggcaataa gcaagaacaa gaaaggaaag ataagcaaga agaaaaaatt tcaagagatt
      901 actatgcacg agatttggaa aatgtcgaaa cagtgataga aaaagaagat gttgaaacca
      961 atgcttcaaa tggtcagaga gttgatttat caagtgaact agataaacta aagaaacttg
     1021 aaaacgcaac agttcacatg gagtttaagc cagatgccaa ggctccagca ttctataatc
     1081 tcttttctgt gtcaagtgct actaaaaaag atgagtactt cactatggca gtttacaata
     1141 atactgctac tctagagggg cgtggttcgg atgggaaaca gttttacggt aattacaacg
     1201 atgcaccctt aaaagttaaa ccaggtcagt ggaattctgt gactttcaca gttgaaaaac
     1261 cgacaccaga actacctaaa ggccgagtgc gcctctacgt aaacggggta ttatctcgaa
     1321 caagtctgaa atctggcaat ttcattaaag atatgccaga tgtaacgcat gtacaaatcg
     1381 gagcaaccaa gcgtgccaac aatacggttt gggggtcaaa tctacagatt cggaatctca
     1441 ctgtgtataa tcgtgcttta acaccagaag aggtacaaaa acgtagtcaa ctttttaaac
     1501 gctcagattt agaaaaaaaa ctacctgaag gagcggcttt aacagagaaa acggacatat
     1561 tcgaaagcgg gcgtaacggt aaaccaaata aagatggaat caagagttat cgtattccag
     1621 cacttctcaa gacagataaa ggaactttga tcgcaggtgc agatgaacgc cgtctccatt
     1681 cgagtgactg gggtgatatc ggtatggtca tcagacgtag tgaagataat ggtaaaactt
     1741 ggggtgaccg agtaaccatt accaacttac gtgacaatcc aaaagcttct gacccatcga
     1801 tcggttcacc agtgaatatc gatatggtgt tggttcaaga tcctgaaacc aaacgaatct
     1861 tttctatcta tgacatgttc ccagaaggga agggaatctt tggaatgtct tcacaaaaag
     1921 aagaagccta caaaaaaatc gatggaaaaa cctatcaaat cctctatcgt gaaggagaaa
     1981 agggagctta taccattcga gaaaatggta ctgtctatac accagatggt aaggcgacag
     2041 actatcgcgt tgttgtagat cctgttaaac cagcctatag cgacaagggt gatctataca
     2101 agggtaacca attactaggc aatatctact tcacaacaaa caaaacttct ccatttagaa
     2161 ttgccaagga tagctatcta tggatgtcct acagtgatga cgacgggaag acatggtcag
     2221 cgcctcaaga tattactccg atggtcaaag ccgattggat gaaattcttg ggtgtaggtc
     2281 ctggaacagg aattgtactt cggaatgggc ctcacaaggg acggattttg ataccggttt
     2341 atacgactaa taatgtatct cacttaaatg gctcgcaatc ttctcgtgtc atctattcag
     2401 atgatcatgg aaaaacttgg catgctggag aagcggtcaa cgataaccgc caggtagacg
     2461 gtcaaaagat ccactcttct acgatgaaca atagacgtgc gcaaaataca gaatcaacgg
     2521 tggtacaact aaacaatgga gatgttaaac tctttatgcg tggtttgact ggagatcttc
     2581 aggttgctac aagtaaagac ggaggagtga cttgggagaa ggatatcaaa cgttatccac
     2641 aggttaaaga tgtctatgtt caaatgtctg ctatccatac gatgcataat ggaaaagaat
     2701 acattatcct cagcaatgca ggtggaccga atcgtgaaaa tgggatggtc cacttggcac
     2761 gtgtcgaaga aaatggtgag ttgacttggc tcaaacacaa tccaattcaa aaaggagagt
     2821 ttgcctataa ttcgctccaa gaattaggaa atggggagta tggcatcttg tatgaacata
     2881 ctgaaaaagg acaaaatgcc tataccctat catttagaaa atttaattgg gaatttttga
     2941 gcaaagatct gatttctcct accgaagcga aagtgaagcg aactagagag atgggcaaag
     3001 gagttattgg cttggagttt gactcagaag tattggtcaa caaggctcca acccttcaat
     3061 tggcaaatgg taaaacagcg actttcctaa cccagtatga tagcaagacc ttgttgtttg
     3121 cagtagataa ggaagatatc ggacagaaaa ttattggtat agctaaagga agcatcgaaa
     3181 gtatgcataa tcttcctgta aatctagcag gtgccagagt tcctggcgga gtaaatggta
     3241 gcaaagcagc ggtgcatgaa gttccagaat ttacaggggg agttaatggt acagagccag
     3301 ctgttcatga aatcgcagag tataagggat ctgattcgct tgtaactctt actacaaaag
     3361 aagattatac ttacaaagct cctcttgctc agcaggcact tcctgaaaca ggaaacaagg
     3421 agagtgacct cctagcttca ctaggactaa cagctttctt ccttggtctg tttacgctag
     3481 ggaaaaagag agaacaataa gagaagaatt ctaaacattt gattttgtaa aaatagaagg
     3541 agatagcagg ttttcaagcc tgctatcttt ttttgatgac attcaggctg atacgaaatc
     3601 ataagaggtc tgaaactact ttcagagtag tctgttctat aaaatatagt agattgaaat
     3661 aagatgtgaa caactctatc aggaaagtc
//
LOCUS       DAVLVF010000120         3630 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVLVF010000120 DAVLVF010000000
VERSION     DAVLVF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.120"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(207..818)
                     /gene="rpsD"
                     /locus_tag="VYN66_001882"
     CDS             complement(207..818)
                     /gene="rpsD"
                     /locus_tag="VYN66_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV1495857.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1013..2065)
                     /locus_tag="VYN66_001883"
     CDS             complement(1013..2065)
                     /locus_tag="VYN66_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1495858.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2062..2760)
                     /locus_tag="VYN66_001884"
     CDS             complement(2062..2760)
                     /locus_tag="VYN66_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1495859.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGCETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2927..>3630)
                     /locus_tag="VYN66_001885"
     CDS             complement(2927..>3630)
                     /locus_tag="VYN66_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEV1495860.1"
                     /translation="EKAKGEGPFTAGVNHVIPYELFAGDGMLTRLLLKASDKAPWSDN
                     GEAKNPALSPLGENVKTKGQYFYQVALDGNVAGKEKQALIDQFRANGTQTYSATVNVY
                     GNKDGKPDLDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDS
                     MPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALA
                     GLLGGLGLKNKKEEN"
BASE COUNT          996 a          812 c          706 g         1116 t
ORIGIN      
        1 aaaatctatt gaatatgcca taaaaagatt ataccacatt gtgtactatt tttggttcac
       61 tttactatat cactttggac tatatcggtc tagttatgtc taaacgatta tagctggata
      121 ctaagagaaa tcaaaaaagc actaagggag aacgtcccaa agtgcttaat atcaaggcct
      181 caaagcctat cttatttaat aaaatattac aacattttgt tgtagaattc aacgacaagt
      241 gcttcgttga tttctgggtt gatttcgtcg cgttctggca agcgagtcaa tgaaccttcc
      301 aacttttcag cgtcgaatga tacgaatgct ggacgtccaa gagtagcttc tactgcttca
      361 aggattgctg gaactttcaa tgatttttca cgaactgaga tcacttgacc tggagttacg
      421 cggtatgatg ggatatcaac gcgtttcccg tcaacaagga tgtgaccgtg gtttacgaat
      481 tgacgagctt gacgacgagt agtcgcgaga ccaagacggt aaacaacatt atccaaacga
      541 cgttccaaaa gaagcataaa gttgaaacct aggattccac ctttgatttt tgtagcttgt
      601 acgaacaagt tacggaattg tttttcacct acaccgtaag tgaaacgaag tttttgtttt
      661 tcagccaatt gcaaaccata ttctgacaat ttagaacggt tgtttggtcc gtgttgtcct
      721 ggtacatagt tacgacgtgc caattcttta cctgtacctg taagtgaaag gccaaggcga
      781 cgagcttgtt tccaagatgg tcctgtataa cgtgacatat gaatgtcctc ctgatataaa
      841 taatatttcg gtggaaatag tcacttagaa agccctgatt cgtgcagatg cccttcgcct
      901 aaacagccaa ggttacttgt cataagacac ctgttgacga gcttcatgct ttcctgctga
      961 tatttcacac taaattcatt ttaccatgaa tagctggatt tgtaaagggg ttttaggctt
     1021 tattttcact accagagagg ttgagaacga gggtaaaggt acttcctaga ccgtactggc
     1081 tgctgactgt gatttcccca cccaattgat gggccaattc acgcgcaatc gcaagtccta
     1141 atccatgacc acctgtcttc atgttacgcg aagtttcgac acgataaagg cgtttgaaaa
     1201 tattttccaa atcctctggg gcaataccct gcccttcatc ggtcacactg attgaaagct
     1261 ggtccttctc cagcttagcc accacttcca gcttggttcc tggagcagaa tatttaaaag
     1321 cgttatcgac cagattcacc aagatacgag aaagcttagc ataatctccc tcaatccggg
     1381 cagactctgg gattacctgc aagtggacat ctcttctctc ctgctcaatc aaaaactgaa
     1441 attcactcat gcactcaatt aagagcttgt ccagaaaaat actgtcttta ctggtagttt
     1501 ccacctgatt tctagctgtg tttagggtca aaaaattcaa ctcctcaacc agtttattga
     1561 gcctctccgt ctggcgtcca atggttgcta gataatgagc ttgctccgac tccttgataa
     1621 taccatccaa aatcccttct accgtcgctt ggatcgaagt gataggagtc ttaatatcat
     1681 gcgacaactg ggcaatcatc aagccctttt ctcgttcgct ttcttccaag gaatcaaagc
     1741 ttacctgcaa atcatgggac atctcattaa aagtttgccc taattgctga aattctacag
     1801 gaccttgaac ctccaaattt gaaggaaaat ccttggccgc tacccgcttg gcatgctcct
     1861 tgagtttgcc caacgacgta aagactggca ataggagaaa gagactaatc ccagcaccga
     1921 caaggctggc aacgatggtc atcccaagca aaaagtaaat ctcgcctttc gcaatcagca
     1981 ttttttgaac agcccaaaaa acaaccaaaa tggttaagag ggttgaaata atatatccaa
     2041 ccaaaatata actttttagt ttcatgtttg tcctctcggt ttctctatct tatatcccaa
     2101 cccccaaact gtcttaatag tgggcgtttg gtcactacta tattttgcca gctcctgtcg
     2161 aagagcatgg atatgcacat tcaaggtatt ggtgtcatcc acgtagtctt ctttccagat
     2221 cttttcatag aggtctgtct tggagaaaac tcgctctgga ttgctagcta aaatccacag
     2281 caattcaaat gatttaacag ttaaatccag catttcttct cctatttgaa cttcatgact
     2341 actatgattc atttttagat tgccaaggga aatcagctct gtttcgcacc cacgatgaag
     2401 gcggcgcaaa atattgtgga cacgcaaaac tagctcacgt gggctaaaag gcttagcaat
     2461 aaaatcatct gctcccaagc tcaggccgta aatcttgtcc tgttcactgg tcttagcagt
     2521 aataaatagg aaaggctgct ctggtgataa gtattgaacc tcactgatta aatcataacc
     2581 atccatccga ggcatcatga catctgtgat aatcaaatca atcggttttt tcttgaacag
     2641 ctctagcgct tccagtccat catgggctac caagacctga taacctgcct gaattaagta
     2701 tctctgatga atatctgtga tttctacctc gtcgtcaacg agtaaaattg tctttcccat
     2761 ctgtctctcc tttgataaaa acagtgctat actgctttaa gtataacact atttttattg
     2821 ttttacgatg attttaatct gctattgaaa gaaagtcctg attgctgatt gagttgttaa
     2881 actaatctta gtcagcccac tatccatcat ttatttcctt agctgattag ttttcttctt
     2941 ttttgttttt caaacctaga ccaccgagta aacctgcaag cgcaagccca aggaaaccaa
     3001 tacttgccat tgatgtttga gtctcaccag tatttggtag catagcttta tcctctgaca
     3061 tcatcgtatc agacatcttg ttagcagaag cagccatgtt ttcacctgcc atcgtgttgg
     3121 tagaacttgt catggtgtca gcaggcatgc tatctgtagt acctgtagca tgattgtgat
     3181 tcatcggagt cacgccagaa ccagagttag aaggtgataa tgaaccattt gctgtgtctg
     3241 atgtttcttt aacatttatc ttaatagtga cttttttagt tgctacgatg ttgtccaagt
     3301 ctggtttacc gtctttgtta ccatagacat tgactgtagc gctgtaagtt tgagtaccgt
     3361 ttgctcggaa ctggtcaatg agcgcttgtt tttctttgcc agctacattt ccgtccaagg
     3421 ctacttgata gaagtattga cctttggtct tcacgttttc gcctagtgga gatagggctg
     3481 ggtttttagc ctcaccgtta tctgaccatg gtgccttgtc agatgccttg agcaagagac
     3541 gagtcaacat gccatcacct gcgaagagtt cgtatggaat cacatggttg acacctgctg
     3601 tgaatggacc ttcacccttg gctttttcta
//
LOCUS       DAVLVF010000121         3483 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVLVF010000121 DAVLVF010000000
VERSION     DAVLVF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.121"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..1210
                     /locus_tag="VYN66_001886"
                     /pseudo
     CDS             1..1210
                     /locus_tag="VYN66_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037353.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            1425..2831
                     /gene="lacG"
                     /locus_tag="VYN66_001887"
     CDS             1425..2831
                     /gene="lacG"
                     /locus_tag="VYN66_001887"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1495861.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            2921..3133
                     /locus_tag="VYN66_001888"
     CDS             2921..3133
                     /locus_tag="VYN66_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV1495862.1"
                     /translation="MNFEKCSQIPCLTSEELKSLGKWYVSTGKEWICHSDDELEEFKN
                     LFLNFINPEEWDTISFDSDFMPFQQS"
BASE COUNT         1087 a          596 c          691 g         1109 t
ORIGIN      
        1 agacttcctg aagaggttcc accaaatatt gcacaagttt ttaaagatgt tattccgttt
       61 gccttatctg ttctttcaat atatggattg gatttaattg ttagaaatat ttttggtaca
      121 aatgtagcag aatctgttgg taaaatatta gcccctctat tttcagctac tgatggttat
      181 atcggtttag ctattgtatt tggtgcttat gctttctttt ggtttgttgg aattcatggg
      241 ccatctgttg tagaaccttt aattgtagct atcagttatg caaatataga agctaatgtt
      301 cagcttgtgc aagctggtat gcacgcagat aaaattttaa atccagttac tcaaactttt
      361 gttgtaacaa acaatgggtg gtactggtgc aacactagta gttccattta tgtttatgtg
      421 gttatgtaaa tcaaaaagaa atagaattgt cggacgtgcg tctgtagttc ctactttctt
      481 tggcgtaaat gaaccaatcc tatttggtgc tccaattgtt ttaaatccta tcttctttat
      541 tccgtttgtt actgctccta ttatcaatgt ttggattatg aaattttttg tagatgttct
      601 acaaatgaat agtttcagta ttattttacc ttggacaaca cctgctccaa ttggtattgt
      661 tatgggaacg gcgctggctc cattgtcatt cgtcttggct ataactttaa tcattatcga
      721 cactttaatc tattatccat ttgttaaagt ttatgaccat caaattttag aagaagaacg
      781 gaaaggaaac tcttcatctg aattgaaaga aaaagttgct gcaaacttca acactgcaaa
      841 agcggatgct attcttgaaa aagcaggtgt cgatgcagca caaaatacca tcactgaaga
      901 aacaaatgtc ctcgttctct gtgctggtgg aggtacaagt ggactccttg caaatgcttt
      961 gaataaggca gcagcagaat acaatgtccc tgtgaaagca gcagcaggcg gctatggtgc
     1021 tcaccgtgaa atgttaccag agtttgattt ggttatcctt gctcctcaag ttgcttcaaa
     1081 ctttgaagat atgaaggcag aaacagataa gctcggtatt aaactagcga aaacagaagg
     1141 cgctcaatac atcaaattaa ctcgtgatgg aaaaggtgcc ctagcattcg tacaagaaca
     1201 attcgattaa ggctaggaac tgtgaaacag tctcctatcg ttacggaaat cgctatggcg
     1261 aatttcctaa tcgccttatt aattcgtcgg taaaaagata tcgtttttac ctcctcatgt
     1321 cacaattcgg tgacttggta caagaagtga gatggagaag gatggctcac tgactcctct
     1381 cctctcactt ttactttatt taaatcaaga aataggtgaa aaaaatgaca aaaacacttc
     1441 caaaagactt tatttttggt ggcgcaacag ctgcctatca agcagaaggt gctacacata
     1501 ctgatggtaa aggaccagta gcgtgggata aatatcttga agataactac tggtacactg
     1561 cagaaccagc cagtgatttt tacaatcgat atccagttga tctcaagcta gcagaagagt
     1621 atggtgtcaa tggcattcga atttctattg cctggtcacg tattttcccg actggttatg
     1681 gtcaagtaaa tgctaaaggt gttgagtttt atcataattt atttgcagag tgtcacaaac
     1741 gtcatgttga gccctttgta actcttcatc actttgacac gccagaagct ctccactcaa
     1801 atggagactt cttaaaccgt gaaaatatcg aacattttgt agactacgct gccttctgtt
     1861 ttgaagaatt tccagaagta aactattgga caaccttcaa tgaaattgga ccaatcggtg
     1921 atggtcaata tttggttggg aaattccctc caggtatcca gtacgacctt gccaaagtct
     1981 ttcaatcaca ccacaatatg atggtgtctc atgcatgcgc ggtaaaattg tacaaagaga
     2041 aaggctataa aggggaaatt ggtgttgttc acgccctgcc tactaaatat cctctagatc
     2101 ctgaaaatcc agcagatgtt cgtgcagcag agttggaaga tatcatccac aataaattca
     2161 tcttagacgc aacttatcta ggtcgctatt cagctgaaac catggaaggt gtcaaccata
     2221 tcttatcagt caatggtggt agtttggatc ttcgtgaaga agattttaca gcattagaag
     2281 ctgcaaaaga cttgaatgat ttcctaggaa tcaactacta tatgagtgac tggatggaag
     2341 cctttgatgg agaaactgaa attatccata atggtaaagg tgaaaaagga agctctaagt
     2401 atcaaatcaa aggtattggt cgtcgtgtag ctcctgacta tgtatcacgc acggattggg
     2461 attggattat ctaccctcaa ggtttgtatg accaaatcat gcgtgtgaag aaagattatc
     2521 ctaactacaa gaagatttac atcactgaaa atggtctcgg ctataaagat gagttcgttg
     2581 ataacactgt ttacgatgat ggtcgtattg attacgtgaa acaacacttg gagatcttgt
     2641 ctgatgcgat tgcagatgga gctaatgtaa aaggttactt catttggtca ttaatggatg
     2701 tcttctcatg gtcaaacggt tatgagaaac gttatggtct cttctacgta gattttgaaa
     2761 ctcaagaacg ttatcctaag aaatcagctc actggtacaa gaaagtagcg gaaactcaga
     2821 ttatagacta gtagaattag tcattagata tagaatttta gtgagtcaaa aagatgttca
     2881 aagattttat ccaatctatt tatgaaaaaa agtttatatt ataaatttcg aaaaatgctc
     2941 tcaaataccg tgtttgacga gtgaagaatt gaaaagtctt ggaaaatggt atgtctcgac
     3001 tggtaaagaa tggatttgtc attcagatga tgagctggaa gaatttaaaa atctattttt
     3061 aaattttatc aatcctgaag aatgggatac tatctccttt gattcagatt ttatgccgtt
     3121 tcaacaatcg taaccaattt ctcaaaaaag ttaaatctta tatttagtac tctgtaaaac
     3181 tcttatctaa tcacgttgct tatactcaat gaaaatcaaa gagcaacttt aaactaggaa
     3241 gcgagtcgca gatttctcaa tgcatagctt tgaggaattg ggcaaaaagt ctttgatata
     3301 gaaaaaagca tagtatcagg tgttgaaaca cctgatacta tgctttttat tgtgggaaga
     3361 tttacttttt ttcttctgaa attgagttgt tacccaggct ctttcagttt attaaggctt
     3421 gatgacttta atgtgtttag atagcttaaa aaggattgaa tcacttagtt tagaatctga
     3481 aac
//
LOCUS       DAVLVF010000122         3376 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVLVF010000122 DAVLVF010000000
VERSION     DAVLVF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.122"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            109..2742
                     /gene="polA"
                     /locus_tag="VYN66_001889"
     CDS             109..2742
                     /gene="polA"
                     /locus_tag="VYN66_001889"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV1495863.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEYGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAKGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCEDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPDIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2827..3264
                     /locus_tag="VYN66_001890"
     CDS             2827..3264
                     /locus_tag="VYN66_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV1495864.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT          915 a          596 c          865 g         1000 t
ORIGIN      
        1 gtttactaaa ttctctggct ctatctattt attcggaaaa agtttgaaaa atacttgctc
       61 tagctcttcc caatggtatt ttttgattct ttcctttata atgggtgtat ggataagaaa
      121 aaattattat tgattgatgg gtcttctgta gcttttcggg cgttttttgc gctgtatcag
      181 cagttggacc gttttaagaa tgcggctggt ttgcatacca atgcgattta tggttttcag
      241 ttgatgttga gtcatttgtt ggagcgggtt gagccgagtc atattttggt ggcttttgat
      301 gcgggaaaga cgaccttccg gacagagatg tatgcggact ataagggtgg tcgggccaag
      361 actcctgatg agtttcgtga gcaatttcct ttcattcgtg agttgctgga tcatatgggg
      421 attcgtcact atgagctggc tcagtatgag gcggatgaca tcattgggac gctggataag
      481 ctagcagagc aggatggttt tgatattacc attgtcagtg gggacaagga tttgattcag
      541 ctgacggatg agcatacggt ggttgaaatt tccaagaaag gtgtggctga gtttgaggcc
      601 tttacgccag attacctcat ggaagaaatg ggcctcacac cagctcagtt tatcgatctc
      661 aaggcgctca tgggtgataa gtcggataat atccctgggg tgaccaaagt cggtgaaaag
      721 acgggtatta agctcttgct ggagtatggt tcgcttgagg ggatttatga aaatattgat
      781 ggaatgaaga cttctaagat gaaggaaaat ctcatcaatg acaaggaaca ggcctttttg
      841 tctaaaacac tagcgaccat tgataccaag gcaccgattg cgattggttt agaggacttg
      901 gtctatagtg gtccagatgt tgaaaatctt gggaaatttt acgatgagat gggattcaaa
      961 cagctcaagc aggctttaaa tgtgtcgtca gctgatgtgg ctaagggttt ggattttact
     1021 attgttgacc aaatcagtca agatatgctg agtgaagagt ctatcttcca ctttgagctt
     1081 tttggtgaga attaccatac ggataatttg gttggatttg cctggtcttg tgaggataag
     1141 ctctatgcca cagacaagct tgagctgttg caagacccga ttttcaagga tttcttagaa
     1201 aaaacatctc tgagagttta tgactttaag aaggttaaag ttcttttgca acgttttggt
     1261 gtggatttgc aggcgcctgc ttttgacatc cgtttggcta aatacctcct ttcgactgtg
     1321 gaggacaatg aaattgcgac catcgctagt ctttatggtc agacttactt ggttgatgat
     1381 gaaactttct acggtaaggg tgttaaaaag gccattcctg aacgtgagaa attcttggaa
     1441 cacttagctt gtaaacttgc tgttttggta gaaacagagc ctattttact tgaaaaactc
     1501 agcgaaaatg ggcaattaga gcttctttat gatatggagc aacctctggc ttttgttctt
     1561 gccaagatgg aaattgctgg gattgtggtc aagaaagaga ccttgcttga gatgcaggct
     1621 gaaaatgagc ttgtcattga aaaactgact caagagattt acgagctggc tggtgaggag
     1681 tttaatgtca actcgcctaa gcagttgggc gtgcttctct ttgagaaatt gggacttcct
     1741 ctagaataca ctaagaaaac caagacaggt tattcgacag cagtggatgt gttggagcgt
     1801 ctcgctccta ttgctccgat tgttaagaaa atcctggatt accgtcaaat tgctaagatt
     1861 caatctactt atgtaattgg cttgcaggac tggattttgg ctgatggaaa gattcatact
     1921 cgctatatgc aggatttgac ccagaccggg cgtttgtcta gtgtggatcc aaacttgcaa
     1981 aatattcctg cccgattgga acaagggcgc ttgattcgga aggcttttgt gccagagtgg
     2041 gaggatagtg tgctactcag ctctgactat tcacagattg aattgcgcgt tttggcgcat
     2101 atttctaagg atgagcactt gattaaggcc ttccaagagg gggcagatat ccatacttcg
     2161 acagccatgc gggtctttgg cattgagcgt cctgataatg tgactgcaaa tgaccgtcgc
     2221 aatgccaagg cagttaactt tggagtggtt tatgggattt cagactttgg cttgtctaat
     2281 aatttgggaa ttagtcgtaa ggaagccaaa gcctacattg atacctactt tgaacgtttt
     2341 ccagatatta aaaactacat ggatgaagtg gtgcgggagg cgcgtgataa gggctatgta
     2401 gagaccctct ttaagcgtcg ccgtgagttg ccagatatca attcgcgcaa cttcaatatt
     2461 cgtggttttg cggagcgaac tgctatcaac tcacctatcc agggttcggc agcagatatt
     2521 ctcaagattg ccatgattca gctggataaa gccttggttg caggtggtta tcagactaag
     2581 atgctgttac aagtgcacga tgaaatcgtc cttgaagtgc ctaaatctga attggtagag
     2641 atgaaaaaat tggtgaaaca aaccatggaa gaagccattc aactcagtgt tcctcttatc
     2701 gcagatgaga atgaaggggc aacctggtac gaggctaaat aaaaaggggg ctagtcctcc
     2761 ttttttgtag tataattctg caaacctttt caaaatgtgc tatactgatg aaaaaggagg
     2821 atttctatga gtcaagaatt tatcaatcca agtgatggcg tgattcgtca gtatctcgca
     2881 acgagtaaaa cccttgctgt ggtgggtttg tctgaccgtg aagaaacaac cagcaatcga
     2941 gtaacaaagg aaatgcaggc tcggggctat aaaatcatcc cagtcaatcc caaggcggca
     3001 ggtggcgaaa tcttgggtga aaaggcttat gctagcctcg ctgaaattcc ttttcctgta
     3061 gatattgtca atgtttatcg tcgcagtgaa tttctgcccg atgtggcgcg tgattttctc
     3121 aaagctgatg ccaagatttt ttgggcacag ctaggacttg aaagtttaga agcgaaagag
     3181 attttgcgtg atggtggatg tgatgatatc gtgatgaatc gctgtatcaa gagagaacat
     3241 actcgcttga ttgaggaagc ataagaaaaa ggtagctgat gggctacctt ttgtgttata
     3301 ctcaatgaaa atcaaagagc acactagcaa actaggaaac tagccgcggg ctgctcaaaa
     3361 caccgttttg aggttg
//
LOCUS       DAVLVF010000123         3304 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVLVF010000123 DAVLVF010000000
VERSION     DAVLVF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.123"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            371..532
                     /locus_tag="VYN66_001891"
     CDS             371..532
                     /locus_tag="VYN66_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495865.1"
                     /translation="MNNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            624..1472
                     /locus_tag="VYN66_001892"
     CDS             624..1472
                     /locus_tag="VYN66_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1495866.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLGAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVW"
     gene            1518..2303
                     /locus_tag="VYN66_001893"
     CDS             1518..2303
                     /locus_tag="VYN66_001893"
                     /inference="COORDINATES: protein motif:HMM:NF024800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
                     /protein_id="HEV1495867.1"
                     /translation="MNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGTKYKDFY
                     YYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKINMKVDTS
                     EIEREIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMYDKLDEL
                     ESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISALISEIQVYE
                     EKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            2571..3137
                     /locus_tag="VYN66_001894"
     CDS             2571..3137
                     /locus_tag="VYN66_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495868.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
BASE COUNT         1250 a          421 c          710 g          923 t
ORIGIN      
        1 aaattttaaa aagattaata actttcgata aattttgaac cttaacaatt gaatagacca
       61 agacaagacg ttaattattt ttggagcgta ataacctatc cgtcaagatc tttcggctga
      121 ataattcggc aaaaaaagtt ctgctaaaca aaaagaaagt gagcgataag taagagtttg
      181 aatataggga gggataccct atccgctatg aagaaaatga ggataatgat acttctaagg
      241 ttgaagccgg ctcatccgga atagaggcgg aggtgagatt cctatgttgc taatccaaag
      301 cacttgtcaa tgtcaaaaaa cttaattaaa caaataatta ttagatataa aactttagag
      361 gaggtatata ttgaacaatg actggaatgg attggctgat ttaatagcaa atctgattgc
      421 aaaatatgct ggtgctttag atttagataa tctacccgat ccaacgccag caaaaaatca
      481 agagatgaaa aatagttttg acatggcaaa aacacaaatt gagacggact aataaaagtg
      541 ttataatata cttgaaatga tagtccaaac ttaaattaaa aaaattatat atgatataga
      601 aaaattatat tgaggttaat actatgtcaa aagaaaaaat aaaagtatac ctctatacac
      661 gagtatctac gtcaatacag atagaggggt attcgttagg ggcacaaaaa tcacgaatga
      721 aagcttttgc tatttacaac gattatgaaa ttgttggaga gtatgaagat gccgggaagt
      781 ctggcaagtc tattgaaggt agaatacagt tcaatcgaat gatggaagat ataaaatctg
      841 gaaaagatgg agtatctttt gttcttgtgt ttaagttatc aagatttgca agaaatgccg
      901 ctgatgttct ttctacactt cagataatgc aggattatgg agttaatctt atctgcgttg
      961 aagatggcat tgattcatct aaagatgctg ggaaactaat gatttctgtt ttatcagctg
     1021 tggctgaaat agaaagagaa aacatccgta ttcaaacaat ggaaggttgc attcaaaaag
     1081 caagggaagg aaaatggaat ggtggctttg ctccgtatgg atataaactt gaagacggca
     1141 agctgtttat aaatgaggaa gaggcagttg caataagaac gattttcgac cagtatgtaa
     1201 atactacgat aggagccaat gggatatcta aatacttaga gaatcatgga attagaaaaa
     1261 tcccaagaca gaatggtaag aatcctttgt ttgatgcagg tcttataaga aagatattaa
     1321 agaatcctgt atataatggg aaaatagcat ttggaagaag aactttagaa aaagttcatg
     1381 gtacaagaaa tgaatataag caggttgaac aagatgaata tctaatatct gaagggatac
     1441 atgaagctat agtttccgat gaagtttggt aagctgctca ggttaagcta aaatctcaag
     1501 caaagaaata tgagcatgtg aataaaggaa aagatacacg cacacacttg ctttctggaa
     1561 ttgtaaaatg cccgatatgt ggagtgggaa tgtttgggaa caagtgtatc aagaaaaaga
     1621 aagatggcac aaagtataaa gatttttatt actatggttg taaacatagg cagatgataa
     1681 gaggtcataa gtgtacattc agtaagcaaa ttagagaaga attgttagat gatgctgttg
     1741 cagaggtgat tgtcaagata gtaagtaatc cgaaatttgc ttctatgatg caagaaaaaa
     1801 tcaacatgaa ggtggatacc tctgaaatag aaagagaaat agataattac caaaaggaat
     1861 tgagaaagag tcattctaca aagtttaagc taattgagga aatagataat ttagatgttg
     1921 aagataagca ttataagaga aggaaacagg atttagatga tagactatat cgtatgtatg
     1981 acaaactaga tgaattagaa tcatcactaa ttgatgcgaa agcaaagaaa cagactattg
     2041 aagctgaaaa gcttacagga gataacatat ataaggttct gatctatttt gataagctct
     2101 ataaagtcat gaatgatgta gagcgtaggc agttaatttc agctttgatt tctgaaattc
     2161 aagtttatga agaaaaacaa tctaacggac aatggctaaa atccattact tttaagctac
     2221 caattataga agaaaatctc aacataggtt tggacaatga tgaacaagtt gaatgtgtaa
     2281 gtttattaga gaaacgtagt taatcctcaa aaggttcccc gaaccttttg agtcccactg
     2341 acgcatcacg ctgaggcggt atcactgctt gtacgagttg aagcatcagc aaagtaaaag
     2401 tagatgttca gccaatgcga cgaggtgaga ccgaaggtcg ataaggtgtg ttctgggtat
     2461 aagactcgta aagtaagtag gaaatatgat gagatgatac agagagcctg aattggtcag
     2521 gtgacgtgct tatttacatt agcctgataa tgaaaatgga gagattatcg atggttatat
     2581 tggaattgta ccaaaatgat tattctaaag atttagtagc atttgattcc atagaagatg
     2641 ggaaagcctt tgtagctcaa atcccgggat acactctgga aacagaagat ggttttgaag
     2701 tggagtattt taatcctaaa aacataccgg attatatgga aattattttt aacggaaata
     2761 ttgttccttt atctaaattt atgttcgatc ctgaggaaaa tgtaaacatt atttggaaag
     2821 agatttcaaa tttatccctc aaaaatgata gagtgatcga aggatattca aaaattgatg
     2881 catatgtcgt taacaaccat gaagttaaag cctatgtcga aacaagagaa acgaactatc
     2941 gtaaagcaaa agacttctta gaaagccgcg gatatgaaat cgacagaagc tttttcggaa
     3001 gtgaagacgg tgaagcgatt ctttacagaa aaaaagggat cgaagtttgg catttcttgt
     3061 gtcacttaga cccaatgttt gtagagattg aagatgtaga aggatatgtt aaagaagaag
     3121 ttggagagat tcaatagtca accaaagttc cttgtgagta tatacctttg ctagtaaaag
     3181 agtcaaaacg ttataacgaa gggggggaag aaaacccttg agtgtaagtc tttttcttgt
     3241 ttagagggaa agtattagag tagccgctca taatttatac tagatttgat aactgatttg
     3301 agac
//
LOCUS       DAVLVF010000124         3273 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVLVF010000124 DAVLVF010000000
VERSION     DAVLVF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.124"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            52..741
                     /locus_tag="VYN66_001895"
     CDS             52..741
                     /locus_tag="VYN66_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1495869.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPK
                     EIAQRFINVIK"
     gene            766..1683
                     /locus_tag="VYN66_001896"
     CDS             766..1683
                     /locus_tag="VYN66_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEV1495870.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            1711..3252
                     /locus_tag="VYN66_001897"
     CDS             1711..3252
                     /locus_tag="VYN66_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV1495871.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGNSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGAIAIVYSYTGGLKSVLWTDFIQGVVLISGVVVALFVLMANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLAIATVFYLIGTGLYVFYQVQNAGSAASNIPQDQIFMYFIAYQLPVGVTGL
                     ILAAIYAAAQSTVSTGLNSVATSWTLDIQDVISKNMSEDRRTKIAQFVSLAVGLFSIV
                     VSIIMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTENKVSAPKYTTIHDISEIKADSS
                     WEVRH"
BASE COUNT         1035 a          497 c          654 g         1087 t
ORIGIN      
        1 agtagatgtt attgaattag taaaaaaaat aaaaaaacgg aggttttctg aatggaaact
       61 aaaaaaatta aaaatttaaa aggtcaaata attgtttcct gccaagcgtt agaaggagag
      121 ccactttata ctccaaatgg aggagtgatg ccgttattag caaaagccgc ctttcaagca
      181 ggcgctaaag gtattcgtgc taattctgtc cgcgatattt cagaaataaa agaagaagtt
      241 gacttaccaa ttataggtat cataaaaaga gattacgatg gctttgaacc ttttatttca
      301 gcaactatga aagagattga tgaattagtt agtgaaggag tagacatttt agctttggat
      361 tgtactaatc gttcaagacc aggttatgat aatattactg actttattca tgatattaaa
      421 gtcaagtatc caaaccaatt attaatggct gatatttcta cttttgaaga agggaaagta
      481 gctgctgaat cgggagtaga ttttgtgggt acgacattat ctggttatac accatatagc
      541 cctaaaaaag ataatcctga ttttgagtta gttgaaagat tggtaaaaga gcttgacgta
      601 ccagttattg ctgaaggaag aataagtact ccggaacaag ctagaaaaat gttagattta
      661 ggtgcttacg cagtggttgt tggaggagct atcactcgtc caaaagaaat tgctcagcga
      721 tttattaatg tcatcaaata aaaaacaata taaaggagaa atctgatgaa agatttaact
      781 aaatacaaag gcgttattcc tgcattttat gcttgctatg atgaaaatgg tgaaattagc
      841 caagaacgtg taaaatctct ggtgcaatat ttcattgaca aaggtgtaaa aggtatctat
      901 gtaaatggtt cttcaggtga atgtatttac caaagtgtag aagatcgtaa acaaattatt
      961 gaagctgtta tggaagttgc taaaggtaaa ttaacagtta tcaaccatgt tgcatgtaat
     1021 aacacgaaag atagtattga attggcaaaa cattcagaaa gtgttggagt cgatgctatt
     1081 gctgctatcc cacctattta cttcaaattg ccagaatatt caattgcagc atattggaat
     1141 gcaatgagtg aagctgcgcc aaatacagat tttatcattt ataatattcc acaattggca
     1201 ggggttgcgt tgactggtag tttgtatgca acaatgcgtc aaaatcctcg tgtgattgga
     1261 gttaaaaatt cttctatgcc tgtacaagat attcaaatgt ttgtagctgc aggtggagaa
     1321 gattacattg tattcaatgg tccagatgaa caatatcttg gtggtcgctt gatgggagca
     1381 gaagctggta ttggtggtac ttatggcgtt atgccagatt tgttcttgaa attggaaagt
     1441 ttgattcaag aacgagattt agatactgct aaaaaacttc aatatgctat caatgaagtt
     1501 atctataaga tgatatcagg taaggcaaat atgtatgctg tagcaaaaga ggttttgcgt
     1561 ctaaatgaaa aacttgattt aggttctgtt cgtcaaccct tagaagcatt ggcagaaggt
     1621 gacttggaag ttgcaaaaca agcagcagaa cttattcaac aagcacgaaa agaattttta
     1681 taataaataa aatcaatatg gagatatgtt atgggcacaa caggatttac aataattgac
     1741 ttaattatct tgattgttta tttacttgcg gtgttggttg caggtatcta tttctctaaa
     1801 aaagagatga aaggaaaaga gttctttaaa ggagatggtt cggttccttg gtatgttact
     1861 tcggtatcca tttttgccac aatgctcagt ccgatttcct tcttgggact cgctggtaac
     1921 tcttatgcag gtagctggat tttatggttt gctcaattag ggatggttgt agctattcca
     1981 ctgacaattc gttttatctt acctatcttt gcacggatag acatcgatac ggcatatgat
     2041 tacttggata aacgttttaa ttctaaagca cttcgtatta tttcagcact cttgtttatt
     2101 atttatcaat tgggacgtat gtctatcatt atgtacctcc catcagctgg tttatcagta
     2161 ttgacaggaa ttgacatcaa tattttgatt attttgatgg gtgcaattgc aattgtttat
     2221 tcttatactg gtggtctaaa atccgtatta tggacagact ttattcaagg tgtggttctg
     2281 attagtggtg tcgttgtagc tttatttgta ctgatggcta atattaaagg tggctttggt
     2341 gcagtagcag aaacattagc aaacgggaaa ttccttgctg caaatgaaaa acttttcgat
     2401 cctaacttgc tttcaaactc catcttctta attgtgatgg gttcaggctt tacaatcttg
     2461 tcttcctatg cttcatctca agatttggtt caacgtttta ctacaacaca aaatattaag
     2521 aaacttaata aaatgttgtt cacaaatggt gtcttgtcac ttgcaattgc aacagtcttt
     2581 tacttgatcg gtacaggctt gtacgtattc tatcaagtac aaaatgcagg tagtgcagct
     2641 agcaatatcc ctcaagacca aatctttatg tactttattg cataccagtt accagtaggt
     2701 gtcacaggtt tgatcttggc agcgatttat gcagcagctc aatcaactgt ttcaacaggt
     2761 ttgaactctg ttgcaacttc atggacattg gatattcaag atgtcatttc taaaaatatg
     2821 tcagaggatc gtcgtacgaa aattgcacaa ttcgtatctc tagcagtagg tttattctca
     2881 attgttgttt ccattatcat ggctcattca gatattaaat ctgcatacga atggttcaat
     2941 agtttcatgg gacttgtact tggtctactt ggtggtgtat tcattcttgg ttttgtttct
     3001 aaaaaagcaa ataaacaagg tgcttatgca gcgctgattg tatcaaccat cgttatggta
     3061 tttattaaat acttccttcc tccaacagct gttagctact gggcatattc attgatttca
     3121 atttctgtat cagtagtttc aggttatatt gtatctgttc ttactgaaaa taaagtatct
     3181 gcacctaaat atacaacgat tcatgatatt tcagaaatta aagcggattc aagttgggaa
     3241 gttcgtcact agtcactaat agaaaagaga aaa
//
LOCUS       DAVLVF010000125         3225 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVLVF010000125 DAVLVF010000000
VERSION     DAVLVF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.125"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            347..1633
                     /locus_tag="VYN66_001898"
     CDS             347..1633
                     /locus_tag="VYN66_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1495872.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAVQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
     gene            1648..1959
                     /locus_tag="VYN66_001899"
     CDS             1648..1959
                     /locus_tag="VYN66_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495873.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            2219..2947
                     /locus_tag="VYN66_001900"
                     /pseudo
     CDS             2219..2947
                     /locus_tag="VYN66_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
BASE COUNT         1009 a          553 c          690 g          973 t
ORIGIN      
        1 aaaaaatcaa ttctaacagt tttatatcag aataataaat aaaaaagcga tattctagac
       61 tgtatcaatc cttttccatt ataggagcaa cagagtggga gtagtcatct aaggactaat
      121 ttatgtattt ttacgagtca gtatcttggg atactggttt ttactttttt agactttttg
      181 actacttgtt aaaactgggg taattttcga ctgtttaaca gttattatgc aaagtctaaa
      241 agattagaat tgtcaaaaca atccgtctag gcttgatttt atcctttatt tactataaaa
      301 tgagaaggaa aaatgtcaaa cttttatatt gcaaatagga gaaatcatga caaaaacatt
      361 aaaacgtcct gaggttttat cacctgcagg gactttagag aagctaaagg tagctgttca
      421 gtatggagca gatgctgtct ttatcggtgg tcaggcctat ggtcttcgta gccgtgcggg
      481 aaactttact ttcgaacaga tggaagaagg cgtgcagttt gcggccaagt atggtgccaa
      541 ggtctatgta gcggctaata tggttatgca cgaaggaaat gaagctggtg ctggtgagtg
      601 gttccgtaaa ctgcgtgata tcgggattgc agcagttatc gtatctgacc cagccttgat
      661 tatgattgca gcgactgaag caccaggcct tgaaatccac ctttctaccc aagccagtgc
      721 cactaactat gaaacccttg agttctggaa agagctaggc ttgactcgtg tcgttttagc
      781 gcgtgaggtt tcaatggaag aattagctga gatccgcaaa cgtacagatg ttgaaattga
      841 agcctttgtc catggagcta tgtgtatttc atactctgga cgttgtactc tttcaaacca
      901 catgagtatg cgtgatgcca accgtggtgg atgttctcag tcatgccgtt ggaaatacga
      961 cctttacgat atgccatttg ggaaagaacg taagagtttg cagggtgaga ttccagaaga
     1021 attttcaatg tcagccgttg acatgtctat gattgaccac attccagata tgattgaaaa
     1081 tggtgtggac agtctaaaaa tcgaaggacg tatgaagtct attcactacg tatcaacagt
     1141 aaccaactgc tacaaggcgg ctgtggatgc ctatcttgaa agtcctgaaa agtttgaagc
     1201 tatcaaacaa gacttggtgg acgagatgtg gaaggttgcc caacgtgaat tggcaacagg
     1261 tttctactac ggtataccat ctgaaaatga gcagttgttt ggtgctcgcc gtaaaattcc
     1321 tgagtacaag tttgtcgctg aagtggtttc ttatgatgat gcggtacaaa cagcaaccat
     1381 tcgtcaacga aacgtcatta acgaagggga ccaagttgag ttttatggtc caggtttccg
     1441 tcattttgaa acctatattg aagatttgca tgatgccaaa ggaaataaaa tcgaccgcgc
     1501 tccaaatcca atggaactat tgactattaa agtcccacaa cccgttcaat caggagacat
     1561 ggttcgtgca ttaaaagaag gactcatcaa tttttataag gaagatggaa ccagcgtcac
     1621 agttcgagct taagaaagga aaaggaaatg atagaggcac agggtttctt agtggataag
     1681 caaacaagat gcattcatta ccatagcaag ctggatatta ttgctttaca atgctatgat
     1741 tgtaaaaagt attatgcttg ttatcggtgt catgattcat tagaaaatca cccttttgag
     1801 ccttatccct tatctttgat acaggataag cctattttat gtggtgtttg tctaaaacta
     1861 ttaacatata agcaatataa agaaagctta agttgcctct tttgtttttc tcgctttaat
     1921 ccaggttgcc aaaatcataa ggaacgctat tttaaatagc aaatcatcta gttttgaagt
     1981 aggagaaaac tcaattccaa gagaaaatga agtaaatctt cccacaataa aacgcataat
     2041 atcaagattg ttcaatacct gatactatgc gtttttaaga ttttaaagac ttttttccta
     2101 gccccttttt actttatctg gtattttgac tacttgttaa aactggggta attttcgact
     2161 gtttaacagt tattatgcaa agtctaaaag gttagaattg tcaaaacaat ccgtctagag
     2221 tatgcgtgat gccaaccgtg gtggatgttc tcagtcatgc cgttggaagt acgaccttta
     2281 cgatatgcca tttgggaaag aacgtaagag tttgcagggt gagattccag aagaattttc
     2341 aatgtcagcc gttgatatgt ctatgattga ccatatctca gatatgattg aaaatggtgt
     2401 ggacagtcta aaaatcgaag gacgtatgga gtctattcac tatgtatcaa cagtaaccaa
     2461 ctgctacaag gcggctgtgg atgcctatct tgaaagtcct gaaaagtttg aagctatcaa
     2521 acaagacttg gtggacgaga tgtggaaggt tgcccaacgt gaactggcta caggatttta
     2581 ctatggtaca ccatctgaaa atgagcagtt gtttggtgct cgtcgtaaaa tccctgagta
     2641 caagtttgtc gctgaagtgg tttcttatga tgatgcgaca caaacagcaa ccattcgtca
     2701 acggaacgtc attaacgaag gggaccaagt tgagttttat ggtccaggtt tccgtcattt
     2761 tgaaacctat attgaagatt tgcatgatgc caaaggcaat aaaatcgacc gcgctccaaa
     2821 tccaatggaa ctattgacta ttaaagtccc acaacttgtt caagctggag acatggttcg
     2881 agctcttaaa gaggggctta tcaatcttta taaggaagat ggaaccagcg tcacagttcg
     2941 tgcttaatgt agttgtttag ttttaaaaaa ttatgcaaag ctccatatac aacacttaaa
     3001 cgagattaaa gaatggcgaa atcccttgat gcgcaagaga ttagctgtcc ttttttattt
     3061 tttaagtgat aaagtcggag cttaggtatt aaagcctatc aaattaaaca aagaagcgat
     3121 gtcttagata ttttgaaaaa aattaataag cagaaaactc tctattattt tgttgtagag
     3181 agttttttgt taataaaatt ttacaaaatg acatttatat attgc
//
LOCUS       DAVLVF010000126         3217 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVLVF010000126 DAVLVF010000000
VERSION     DAVLVF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.126"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            525..3083
                     /locus_tag="VYN66_001901"
     CDS             525..3083
                     /locus_tag="VYN66_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1495874.1"
                     /translation="MERSQFEEMFQLQSSRLTTQEKLQLFTSVFAGRYDVYAKNFINE
                     QGKIQYFPSYDYGWKQLPPEKRSFQTLTNSVLKSHFRGEAAIGIFPMHLDDSCYFLVL
                     DLDEGDWKEAGLTIRRIARERQMEAHLEISRSGYGLHIWFFFEEAILSRKARLFGKKL
                     LELAMQESMQLSFDSFDRMFPNQDVLPKGGFDNLISLPFQGEAYHQGRTVFVDEQFQP
                     YEDQWRYLQEIQKISTAKVALLIQEELGKQELDKELKIVLSNMIQLEKSSVTPKTLFF
                     LKNMASFSNPEFYLKQAMRQPTYQIPERMYLFGESDHYLWLPRGLLYPLQDKFKQVSV
                     EDRRKVQRSISVEFKGELTFEQELALSDMTSKENGLLHAETGFGKTVLGAALISERKT
                     KTIILVHNKQLLDQWLDRLNCFLTFEEEEAIRYTASGREKVIGYVGQYGGTKKWLSKL
                     VDVVMIQSLFKLENSQSLLDEYEMMIVDECHHVSALMFEKVVAQFRGKYLYGLTATPE
                     RKNGHEPIVFQRIGEILHTADKRETDFKRQLQLRFTSFGHLEIEKTKASNFIQLSDWI
                     ATDSARNQLILKDILAQVAEGRNILVLVNRIQQIDVFEKLLKEKEVDGCYIISGKTKV
                     RERTSLLETLEQLDKGFVLLSTGKYIGEGFDLPQLDTLILAAPFSWKNNLIQYAGRIH
                     RNYKDKSLVRIFDYVDIHVPYLEKMFQKRQVAYRKMDYRVIEGEEKQFVYVDSRYEKV
                     LREDLAGERQECLLILPYVHQTKLMNFLKEFRISQIEICIPETVANKAWLDQLKSQKI
                     KVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTILRLESASIVSELVAGLR"
BASE COUNT          979 a          486 c          709 g         1043 t
ORIGIN      
        1 ttgtcgcttt tcattataga tcttatggga ctttttttct acccaaaata ggctccataa
       61 tatccatagg gaatttaccc actacaaata ttatagagcc caaagtttta ggtcgcttga
      121 taatatgcgt tttttgaatt ttatagactg ctcgtttaaa ctctatttac ttcgtacctt
      181 ctggagcgag acggaatatt agtcacatac aaaatgagta ctattaggat tttattttca
      241 tgtacaattt cagccagtct tgttataatc agcctatagg aatcaaggag gtgactctta
      301 tggctgtttt tgtgtctttg gatggaattg tggtagaagt ccttgatgtc ttttcttctt
      361 ttaatgggga tagtgagttt ttcttgtgta tagcattttg aatctggaat aggacgccat
      421 gactgctaaa agatttctat aaattaattt gattttccta atcaatttgt tcatatctta
      481 tttcattcca ctataaacgt cttaaagaca agagtcagtt tgttatggaa cgctctcagt
      541 tcgaggagat gttccaactt caaagtagtc gcttgacgac gcaagaaaaa ttacaattgt
      601 ttacctctgt gtttgctggc cgttatgatg tttatgctaa gaattttatc aatgaacaag
      661 ggaaaattca gtattttcct tcctatgatt atggttggaa gcagttgcca cctgaaaaac
      721 ggagtttcca gacattgacg aactccgttt tgaaatctca ttttcgtggg gaggcagcta
      781 tcggtatctt tcctatgcac ttagatgata gctgttattt tttggtactg gatttggatg
      841 aaggagattg gaaagaagct ggtttaacca ttcgaagaat agccagggaa cgccagatgg
      901 aagcccattt agagatttct cgttcgggtt acggactcca tatttggttc ttctttgagg
      961 aagcgattct gagtcgaaag gctcgcctgt ttggaaagaa actattagaa ttggctatgc
     1021 aggaaagtat gcaactgtcc tttgattctt ttgatcgcat gtttccaaat caggatgttc
     1081 ttcctaaagg tggatttgat aatttaattt cccttccttt tcaaggagaa gcttatcatc
     1141 aagggcgaac ggtctttgtg gatgaacagt ttcagcctta tgaagatcaa tggaggtatc
     1201 tacaagaaat tcagaagatt tcaactgcta aagtggcact gttaatccaa gaggagttag
     1261 gcaagcaaga attggacaag gagttgaaga tcgtcttatc caatatgatc caacttgaaa
     1321 aatcgtctgt gacacccaag acactgtttt tcttgaaaaa tatggcttca ttttctaatc
     1381 ccgaatttta tttaaagcag gctatgcgac agccaaccta tcaaattcct gagagaatgt
     1441 atttatttgg agaatccgat cattatttat ggttgccaag aggtttgctg tatccattgc
     1501 aagataaatt taagcaggta tctgtggaag ataggagaaa ggtacaaagg tctattagcg
     1561 tggaatttaa gggagaactc acttttgagc aagagttagc cctgtcagat atgacttcta
     1621 aagaaaatgg tttacttcat gcggagactg gttttgggaa gaccgtttta ggtgctgctc
     1681 ttatctctga acggaaaaca aaaacaatta ttctagtcca taataagcaa ctcttagacc
     1741 aatggctaga tcgcttaaac tgctttttga ctttcgaaga ggaggaggct atccgttata
     1801 cggcatcagg tcgtgaaaag gtaatcggct atgttggaca gtacggtggg actaagaaat
     1861 ggctgagtaa actggttgat gtcgttatga ttcaatctct atttaagttg gaaaatagtc
     1921 aaagtctttt ggatgagtat gagatgatga ttgtggatga gtgtcatcat gtctctgcct
     1981 tgatgtttga aaaagttgtt gctcagttta gagggaagta tctttacggt ttgacggcta
     2041 cgcctgagcg taagaatggt catgagccta ttgtttttca gagaattggt gagatactcc
     2101 atactgctga taagagggaa acggatttta aacggcaatt gcaattaaga ttcacttctt
     2161 ttggtcattt ggagattgaa aagaccaaag caagtaattt tatacagctc agtgattgga
     2221 ttgctacaga ctcagcgagg aatcagttga ttctcaagga tattctagcc caagtggcag
     2281 aaggacggaa tatcttggtt ttagttaatc gaattcaaca gatagatgtc tttgaaaagt
     2341 tattgaaaga gaaagaggtt gatggctgtt acattattag cggaaaaacc aaagtccgag
     2401 aaagaacgag tttactggag acgttagaac agttagataa agggtttgtt ttgttgtcta
     2461 ctggaaaata cattggcgaa ggttttgact tacctcagtt ggacacgctt atcttggcag
     2521 cacccttttc ttggaaaaat aatttaattc agtatgcagg tcggattcat agaaactaca
     2581 aggataagtc tttggtgcgt attttcgatt atgtggatat tcatgttcct tatttagaaa
     2641 agatgtttca gaaacgacaa gtagcttatc gaaagatgga ttatcgtgtc atcgagggtg
     2701 aggagaaaca attcgtttat gttgatagta gatatgagaa ggtgttgaga gaggacttag
     2761 caggggaaag acaggaatgt ctgcttattt taccttatgt gcaccagaca aaactgatga
     2821 attttctaaa agaatttagg attagtcaaa ttgagatatg tataccagag acggttgcaa
     2881 ataaagcatg gctagaccag ttgaagagcc agaaaattaa agtgtctttt actcaatcaa
     2941 aaatagtaac gcctattctt ttggtgaata agactattgt ttggtatggt gcaatgccat
     3001 tattagggaa ggtagatgag atgaccatat tacgtttgga atcagctagt atagtttctg
     3061 aactagtggc aggtttacga tagagaaaat ttttaaaaat ttctatgtat gattttcatt
     3121 ttttaatgag actgttgaca ttatcacatt cgaagcacac aaaataaaaa aatttctaca
     3181 agtacttgac aaatagattg aaatatctta aaatgaa
//
LOCUS       DAVLVF010000127         3215 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVLVF010000127 DAVLVF010000000
VERSION     DAVLVF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.127"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            21..3140
                     /gene="phtE"
                     /locus_tag="VYN66_001902"
     CDS             21..3140
                     /gene="phtE"
                     /locus_tag="VYN66_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV1495875.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1191 a          536 c          632 g          856 t
ORIGIN      
        1 tttattgaaa tgagggattt atgaaattta gtaaaaaata tatagcagct ggatcagctg
       61 ttatcgtatc cttgagtcta tgtgcctatg cactaaacca gcatcgttcg caggaaaata
      121 aggacaataa tcgtgtctct tatgtggatg gcagccagtc aagtcagaaa agtgaaaact
      181 tgacaccaga ccaggttagc cagaaagaag gaattcaggc tgagcaaatt gtaatcaaaa
      241 ttacagatca gggctatgta acgtcacacg gtgaccacta tcattactat aatgggaaag
      301 ttccttatga tgccctcttt agtgaagaac tcttgatgaa ggatccaaac tatcaactta
      361 aagacgctga tattgtcaat gaagtcaagg gtggttatat catcaaggtc gatggaaaat
      421 attatgtcta cctgaaagat gcagctcatg ctgataatgt tcgaactaaa gatgaaatca
      481 atcgtcaaaa acaagaacat gtcaaagata atgagaaggt taactctaat gttgctgtag
      541 caaggtctca gggacgatat acgacaaatg atggttatgt ctttaatcca gctgatatta
      601 tcgaagatac gggtaatgct tatatcgttc ctcatggagg tcactatcac tacattccca
      661 aaagcgattt atctgctagt gaattagcag cagctaaagc acatctggct ggaaaaaata
      721 tgcaaccgag tcagttaagc tattcttcaa cagctagtga caataacacg caatctgtag
      781 caaaaggatc aactagcaag ccagcaaata aatctgaaaa tctccagagt cttttgaagg
      841 aactctatga ttcacctagc gcccaacgtt acagtgaatc agatggcctg gtctttgacc
      901 ctgctaagat tatcagtcgt acaccaaatg gagttgcgat tccgcatggc gaccattacc
      961 actttattcc ttacagcaag ctttctgcct tagaagaaaa gattgccaga atggtgccta
     1021 tcagtggaac tggttctaca gtttctacaa atgcaaaacc taatgaagta gtgtctagtc
     1081 taggcagtct ttcaagcaat ccttcttctt taacgacaag taaggagctc tcttcagcat
     1141 ctgatggtta tatttttaat ccaaaagata tcgttgaaga aacggctaca gcttatattg
     1201 taagacatgg tgatcatttc cattacattc caaaatcaaa tcaaattggg caaccgactc
     1261 ttccaaacaa tagtctagca acaccttctc catctcttcc aatcaatcca ggaacttcac
     1321 atgagaaaca tgaagaagat ggatacggat ttgatgctaa tcgtattatc gctgaagatg
     1381 aatcaggttt tgtcatgagt cacggagacc acaatcatta tttcttcaag aaggacttga
     1441 cagaagagca aattaaggct gcgcaaaaac atttagagga agttaaaact agtcataatg
     1501 gattagattc tttgtcatct catgaacagg attatccaag taatgccaaa gaaatgaaag
     1561 atttagataa aaaaatcgaa gaaaaaattg ctggcattat gaaacaatat ggtgtcaaac
     1621 gtgaaagtat tgtcgtgaat aaagaaaaaa atgcgattat ttatccgcat ggagatcacc
     1681 atcatgcaga tccgattgat gaacataaac cggttggaat tggtcattct cacagtaact
     1741 atgaactgtt taaacccgaa gaaggagttg ctaaaaaaga agggaataaa gtttatactg
     1801 gagaagaatt aacgaatgtt gttaatttgt taaaaaatag tacgtttaat aatcaaaact
     1861 ttactctagc caatggtcaa aaacgcgttt cttttagttt tccgcctgaa ttggagaaaa
     1921 aattaggtat caatatgcta gtaaaattaa taacaccaga tggaaaagta ttggagaaag
     1981 tatctggtaa agtatttgga gaaggagtag ggaatattgc aaactttgaa ttagatcaac
     2041 cttatttacc aggacaaaca tttaagtata ctatcgcttc aaaagattat ccagaagtaa
     2101 gttatgatgg tacatttaca gttccaacct ctttagctta caaaatggcc agtcaaacga
     2161 ttttctatcc tttccatgca ggggatactt atttaagagt gaaccctcaa tttgcagtgc
     2221 ctaaaggaac tgatgcttta gtcagagtgt ttgatgaatt tcatggaaat gcttatttag
     2281 aaaataacta taaagttggt gaaatcaaat taccgattcc gaaattaaac caaggaacaa
     2341 ccagaacggc cggaaataaa attcctgtaa ccttcatggc aaatgcttat ttggacaatc
     2401 aatcgactta tattgtggaa gtacctatct tggaaaaaga aaatcaaact gataaaccaa
     2461 gtattctacc acaatttaaa aggaataaag cacaagaaaa ctcaaaactt gatgaaaagg
     2521 tagaagaacc aaagactagt gagaaggtag aaaaagaaaa actttctgaa actgggaata
     2581 gtactagtaa ttcaacgtta gaagaagttc ctacagtgga tcctgtacaa gaaaaagtag
     2641 caaaatttgc tgaaagttat gggatgaagc tagaaaatgt cttgtttaat atggacggaa
     2701 caattgaatt atatttacca tcgggagaag tcattaaaaa gaatatggca gattttacag
     2761 gagaagcacc tcaaggaaat ggtgaaaata aaccatctga aaatggaaaa gtatctactg
     2821 gaacagttga gaaccaacca acagaaaata aaccagcaga ttctttacca gaggcaccaa
     2881 acgaaaaacc tgtaaaacca gaaaactcaa cggataatgg aatgttgaat ccagaaggga
     2941 atgtggggag tgaccctatg ttagatccag cattagagga agctccagca gtagatcctg
     3001 tacaagaaaa attagaaaaa tttacagcta gttacggatt aggcttagat agtgttatat
     3061 tcaatatgga tggaacgatt gaattaagat tgccaagtgg agaagtgata aaaaagaatt
     3121 tatctgatct catagcgtaa ggaatagcag tagaaaaagt ctgaatcaaa aatgaagttc
     3181 tctcaaaagt tagaaataaa actctgactt tggga
//
LOCUS       DAVLVF010000128         3163 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVLVF010000128 DAVLVF010000000
VERSION     DAVLVF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.128"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..156
                     /locus_tag="VYN66_001903"
     CDS             1..156
                     /locus_tag="VYN66_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1495876.1"
                     /translation="MINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKGER
                     NITNGFK"
     gene            143..349
                     /locus_tag="VYN66_001904"
     CDS             143..349
                     /locus_tag="VYN66_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495877.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            395..739
                     /locus_tag="VYN66_001905"
     CDS             395..739
                     /locus_tag="VYN66_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495878.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKVMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKK"
     gene            883..1308
                     /locus_tag="VYN66_001906"
     CDS             883..1308
                     /locus_tag="VYN66_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495879.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEHMTTGDILIEAFKA"
     gene            1716..2711
                     /locus_tag="VYN66_001907"
     CDS             1716..2711
                     /locus_tag="VYN66_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495880.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQHILKGKMLEIWKYRPF
                     VSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS"
     gene            2701..>3163
                     /locus_tag="VYN66_001908"
     CDS             2701..>3163
                     /locus_tag="VYN66_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495881.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNSEFPSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCG"
BASE COUNT         1116 a          395 c          645 g         1007 t
ORIGIN      
        1 gtgataaatg tatgtgatgt tggaaaaaga ataaaagaac ttagaatatc ttcaaatctt
       61 actcaagata agattgctga gtatttgtct ttgaatcaaa gcatgattgc caaaatggaa
      121 aaaggtgaaa ggaatatcac gaatggattt aagtaataaa gcttcaaatc ttagaaaaaa
      181 gttgggagct gatggtgaat cgccgataga tatttttaaa ttggtacaaa agatagaaaa
      241 tttgacgctg gtattttatg gactcggaaa gaatattagc ggagtctgtt ataaaggaac
      301 tcagttcagt ctcattgcag tcaattcaga catgtcatta ggaaggtaaa gattttcttt
      361 agcacatgaa ctgtatcatc tttattatga tgaggtgaag aagagttcag tcagtcttat
      421 cttgattggt gaaggagatg aaactgaaag aaaagcggat cagtttgctt cttatttttt
      481 aattttccca tcttcactgt ataggatggt tgaggaaatt agagaaaatg ccaatagaac
      541 tcatcttgaa gtagaagata ttataaaatt gggtcagttt tatggtatca gtcataaagt
      601 tatgttatat agattgagga atgatggata ccttgatgca gaagaaatta aaaatatgga
      661 tattagtgtt atagagacag cttcaagatt aggctatgat acaagtttat atcgtccttt
      721 gtcagaaagt aaaaagtaaa tgatattagg ataatatgtt aattcaactg aacaactttt
      781 agaaaataac agaatttcgc aagggaagta tgaggaactg ttactagatg ctttcagata
      841 tgatattgta tatgggctag atgaagaggg gggagttgtc gtttgactag tcgtgtattt
      901 attgatgcag attgtatttc agtattttta tgggttggta ctgaacatct tttagaaaag
      961 ctctattcgg gtaaaattgt tattccacaa gaggtgtatg atgaaatcaa tatacctaca
     1021 attccccatt taaaatctag gatagatcag ttggtagcta agggttcagc tgagattgtg
     1081 agcatagaca ttggaactga agaatacgca ttatatagag atttaacaag aaatcatgat
     1141 agtaacaaga ttattggtaa gggagaagcg gcttctattt ccttagcgaa aaagcataat
     1201 gggatattag gaagtaataa cctaagagat gttaaatcat atgtagaaga attttcttta
     1261 gaacatatga caacaggaga tatactgatt gaagcgttta aagcgtaatt tattactgaa
     1321 caagagggca atcatatctg gaataatatg cttaaaaaga gaaggaaaat tggtgcaaat
     1381 tcattttcag actatcttcg tggaagtatt catcaaaata gacaaaaata aatttggata
     1441 aatcgaactc actattcagg aggcatatga gcaattcgaa aaagaaaagt gtcaaattga
     1501 gcctatagga gtagaagtga aatagtaagt cctgcatagt ggatgagaga aaagttctcc
     1561 ttgaagtttt cctgaactat cagtcgcatg tcaaacgata tgtagggtaa tgtgagaggg
     1621 gatagggagt agtttttggt tattttatca aaaaacttat attttattat accgaatgat
     1681 aaaatataat aaaaatgata gaataaggaa aaaacatgaa tgtcaaaaag ataatgtcaa
     1741 tttttcaatc cttttatgtt gatgtcagta ttgaggaact gactttgact ttaccaatca
     1801 gttttgtaaa aaggtttgag tatactcaaa tgacttttca taaggaatca tttttattga
     1861 ttaaagaaaa gagaaggggg agtttgagtt catttgttac tcaggctcgc actatgggtg
     1921 aaaaagccaa tatggatgtt gttttggtgt tttcgaagtt atcagacagt gaaaaaaagc
     1981 aattacttca agctagagtt ccgtttgtag actttaaggg aaacctcttc ttccctccat
     2041 tgggactagt actcaatgcg aatgatactg aagtccctaa ggaattaaca cctagcgaac
     2101 aattaacgtg gattgccttt ttattgacaa aaggtcaaaa agtagtagat gttgatttgc
     2161 tttcacaagt cactggactt ccaaactcaa caatttatag gtgtttgagg acttttaaag
     2221 ctttatattg gttaaacaag caaaataagc tttacacata tacggtgtca aagaaagaat
     2281 tattcttaaa atccgtgtca tgtttattta atcccatcaa aaaacggatt ttattgccag
     2341 atggcgatat aaagcagata aaatctgttt ctaaccttct atatggtggt gcttatgctt
     2401 tgtcgcattc aactttttta gctgaaacgg atgaaaatac tagctatgtc atatggcaga
     2461 gaaaattcaa tcagttatcc ttgccacttt ctcagcatat tttaaaagga aagatgctag
     2521 agatatggaa atatcgtcct tttgtatctg agttttggaa tgattttaaa aataatcatg
     2581 ataaacaatt tgtagatccg atttctcttt atttgacctt aaaagatgat gatgacccac
     2641 gtatagagga agagagtgaa gcactagaaa atatgatatt acagtatctg ggagaagatg
     2701 atgccagcta atacgaaagt tatttttcaa gaaatgtttg cggattttca gaactattat
     2761 gttctgattg ggggaactgc tacctctatc gtattggatt cgcaaggatt taaaagtcgc
     2821 acaacaaaag attatgatat ggtcatcatt gatgaagtaa aaaataagga attttatact
     2881 accttgaatc attttttaga attgggagag tatcaaggaa gtcagaaaga tgagaaagcg
     2941 cagctttttc gatttacaac aactaattct gagtttcctt ctatgattga actatttagt
     3001 atcttaccag aatatccatt aaagaaggac ggtcgagaaa ttcccttata ttttgaccaa
     3061 gatgctagtt tatcagcctt attattggat gaagattatt ataatatatt ggtgcatgta
     3121 aaagaaacca ttcaggggta ttcggtattg agtaattgtg gtc
//
LOCUS       DAVLVF010000129         3087 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVLVF010000129 DAVLVF010000000
VERSION     DAVLVF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.129"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..175
                     /locus_tag="VYN66_001909"
     CDS             <1..175
                     /locus_tag="VYN66_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1495882.1"
                     /translation="NRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDTSSSGVTL
                     ITGKNGTGKSTLL"
     gene            complement(541..1851)
                     /locus_tag="VYN66_001910"
     CDS             complement(541..1851)
                     /locus_tag="VYN66_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV1495883.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2088..2960
                     /locus_tag="VYN66_001911"
     CDS             2088..2960
                     /locus_tag="VYN66_001911"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV1495884.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          948 a          518 c          702 g          919 t
ORIGIN      
        1 aaacagaact gtttcttttg ctattagttc aaaaggagaa aaaatgaaag tagaaaatat
       61 ttcgtatagg gtggatcatc gtatattgtt tgataatatt tcttttgata cttcgagttc
      121 aggcgtgaca ttaattactg gtaaaaatgg tacaggaaag tcaactttac tatagtagat
      181 tgaaactaga atagtacaca tctacttcta aaatattgtt agaaatcgat ttgactatcc
      241 tgatctattt gtcctgttct tatttcattt cactatatct caaattgagt atgacgaagt
      301 gcgctcccat gtcctgggaa cgcactttct tcatattttt catattcttg aatccatcga
      361 taaagactat tgggatgaat ttttaaagtt gaactaatca tttttacagg atgagattta
      421 cagcagagag tttgaaggct ttatcaaagg tttttcttgg cataatgact tttcctcgtt
      481 tccacttaat tttgtgtcta ctttattata ccaagtccac ccttaagtta gataataaat
      541 ctaacttaag gaagctagaa ggatgagaat ccaggtggtc aagagtccca aacttaagct
      601 gatggggaca cccagaataa tttgcttttt gaaggcaagg ccacgttcct ctatattggg
      661 aagtgagagt tgaatgagag aaccagctga tgaaaagggt gagatattag tagatagagc
      721 gccaataacg gtggctgttg tgagtaagtg aatatcaatc tgaggatttt gagcactgat
      781 gatagcaatg atgggaaaga gggctggagc tacaacggat agggtggaac taaagagtga
      841 catcactccg gctatcacac aaaagaacag aggtaaccag aaatgaggaa tggttgttgt
      901 catgaggtgc cctatcagtg tgactaaacc tgacttgacc gctagagaca ttagtaagct
      961 catgccgcag agcatgataa ttgtagccca gggaacctta gctaaaatgg cttcttgctt
     1021 ccctaatttg agccttaagg cgaggcagac catgagtatt gagacaaagc caatatcaaa
     1081 tgttttttga taagtagcta tccaggcgat gtttgggaaa atgagatgca acaagggaaa
     1141 aagccaaacc aaaaccatgc tgctgatcat gagcaaggtg gtttgtcttt gaaccttgct
     1201 gaggagtggt ggttggtcaa tagtcaagga tgagtttgtt cttcccttac tatagtgact
     1261 gtaacaggat aataaaagca agacgatgag tgggtagata atgctgacga taaagatatg
     1321 attgccaagt gaaaaagctt gctcttccca tcccatttgc ttaaacaggc cttgaaagac
     1381 aatgcctgag ctactggtta tcaaattagc ccctcctgaa gctccccaat tgacggcttg
     1441 agctccaatc aaagggtgtt tgtccgcttt ttgacagagg gtaatcgcta gaggacagca
     1501 aacggccata gtagtgaaaa atccagcacc taaagcagac aaaagggttg ccatcaggta
     1561 taaaatcatg tagagggcgt tagggtgggt gcgtgtgcgg tagagaatgt gttgagccaa
     1621 aacatcaaga gtaccgttag ttgttgcaac gttataaaag agagagacgc taaaaatggt
     1681 aaaaaagagt gaggttggcc aaaaatgaag aagttctttg gggcttaatc ccatgagagt
     1741 ggttgcgatg aggtaagaaa aagcaatagc cagcaggcca atattgattt tggtgcggta
     1801 accaattcca atggctagag caatggcgct aatcattatt aaatgaatca ttgaattgtc
     1861 ctttcagtta gaatatggaa ataggttggc aattttggcg ctcaaaagac cacctgtttc
     1921 ctattggtac ttgactatta tacacaaaaa aaatacaatt gtcttgacaa ttgtattgac
     1981 acttgttaga attaaaaatg aggtattcag gcttgtgatt tcctatggaa gttaatagta
     2041 attgcctcta gtgcttacaa gtgatattaa aaatagagga cttagtgatg tcaatcattt
     2101 caactgattt aacccctttt caaatagatg atacattgaa agcagccttg cgagaagatg
     2161 ttcattccga agattacagt accaatgcca tttttgatca tcatggccaa gccaaggtgt
     2221 cgctttttgc caaggaagct ggtgttttag cgggactaac cgtttttcaa agggttttta
     2281 ccctatttga tgccgaggtg accttccaga atcctcatca atttaaggat ggggatcgtt
     2341 tgactagtgg cgatttggtt ttagaaatca taggctcggt gagaagtctc ttaacatgtg
     2401 aacgcgttgc cttgaatttt ttacaacatt tatcagggat tgcttcgatg acagctgctt
     2461 atgtagaagc cttaggcgat gattgcatta aggtatttga tactcgaaaa actactccta
     2521 atttacgtct ttttgagaaa tatgccgtga gagttggcgg tggctataat catcgcttta
     2581 atttatcaga tgctatcatg ctaaaagaca atcacattgc ggcagtaggt agtgttcaga
     2641 aggcaattgc tcaagcgcgt gcctatgccc cttttgtgaa aatggtcgag gtggaagtgg
     2701 aaagccttgc tgctgccgaa gaagctgcgg cggcgggtgc tgatattatc atgttggata
     2761 atatgtcatt ggaacagatt gaacaggcca ttaccctaat tgcaggacgt tctcggattg
     2821 aatgttctgg aaatattgat atgaccacta ttagccgttt tcgtggttta gcgattgatt
     2881 acgtctccag tggtagttta acccatagtg ctaagagtct tgatttttcc atgaagggtt
     2941 taacctacct tgatgtctaa gttgtaaaat aaactaactt tttaaaggat gtctttcctc
     3001 tagaacgagt tttatgtcag atagtttaaa cgcctcttca aatatagtaa aatgaaccaa
     3061 aaatagtaca caatgtggta taatctt
//
LOCUS       DAVLVF010000130         3082 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVLVF010000130 DAVLVF010000000
VERSION     DAVLVF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.130"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(140..673)
                     /locus_tag="VYN66_001912"
     CDS             complement(140..673)
                     /locus_tag="VYN66_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV1495885.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(762..1538)
                     /gene="recX"
                     /locus_tag="VYN66_001913"
     CDS             complement(762..1538)
                     /gene="recX"
                     /locus_tag="VYN66_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV1495886.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLSQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1576..2931
                     /gene="rlmD"
                     /locus_tag="VYN66_001914"
     CDS             1576..2931
                     /gene="rlmD"
                     /locus_tag="VYN66_001914"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1495887.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            2993..3064
                     /locus_tag="VYN66_001915"
     tRNA            2993..3064
                     /locus_tag="VYN66_001915"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3026..3028,aa:Glu,seq:ttc)
BASE COUNT          970 a          556 c          577 g          979 t
ORIGIN      
        1 tacgaattct gcataaatct ctttctagta atgtgttgta actctgctat aatggattta
       61 ttcctttttg tatttacaca atttatttta tactaccaaa aaaacaaggt caggattttg
      121 ttcctgacct ttgacaactt taccgattct ttagttctac atagcgcttg taccaaatgt
      181 ttacataggc ttctgagaaa ggaccacgtc cattgttaat ccaatcaaca agaattttga
      241 catgttcttt taaaatatag tccaagtcat cagaataatt cattttgcgt ttgtgacgct
      301 cgtattcttc aacgtccaag agacgttttt ccccatctgt aaaaatttta acatccaaat
      361 cgtaatcaat atacttcagt gcttcttcat ccagatagta ggggctagcc atattgcaat
      421 agtaagaagt tccattatca cgaatcatgg caatgatatt aaaccaatat ttcttgtgaa
      481 agtaaacaat agccggttct cgagtgaccc aacgacgacc atcactttcg gtaacaagtg
      541 tatgatcgtt gacaccaata atggcgtttt ctgttgtttt tagtaccatg gtgtcccgcc
      601 aagttcggtg gagactccca tcatgcttat aactttgaat tgtaataaag tcgccttctt
      661 ttggaagctt cataactaac caactttcta caatttataa gtttatcatt tactattgta
      721 ccataaaatt acctaaaatc tgtgaatttc acttggaaat attaaagata ttctctaaga
      781 gcgcttgcta tatccgaaaa atcgtagccc tttcgtgcta aaacttgagt taaacgctgc
      841 ttcagttcgt atccttcata ctttcgagca tacttagtat attgcttatc aagttccttg
      901 aagatgagtt cttgagtcgt ttcttggtca acttggctat ctaagtcgtc aaaggcaatt
      961 ttagcatcag aataagagaa gcccttgtta gtcaagttct ggataatctt atcttgcagg
     1021 gcacgagctg gaagttttcc ctcatatttt ttcaatagtt tattggctac acgttgagca
     1081 acttcagaaa aatcaaattc tttcaagttc tcttctatag tagattttga aatccctttt
     1141 tgagatagtt tctgagtcag cacataagga cccttgtctc ctgaaagttg attggtattg
     1201 atgatagcat aagcgtactg accatcatta atccacttat cttctttaag attagcaata
     1261 acttgagaaa cgatgttttt atcaatatcg tattttttca gatattctct gacttctttt
     1321 tcagtgcgtg ctttaaagga taagtggtag agagcgagat tcttaccata agaaaattga
     1381 gcaaagtctt gaatctcttt caattcctct tcgcttatca ccttatctct cgataacata
     1441 aaacgaacaa ttgtatcttc ggtgatatag catttgtcgc cattatcaag ctccatcagg
     1501 tagagtcttt ttttcttttc aagttttgtg attttcatag ctctattata actcaaaatg
     1561 tgataagata ggggtatgaa tctgaaagtg aaacaaaaaa taccattaaa aatcaagcgc
     1621 atgggaatta acggtgaggg aatcggcttt taccaaaaaa cattagtctt tgtaccagga
     1681 gctctcaaag gcgaagatat ctattgtcag attacttcta ttagacgcaa ctttgttgaa
     1741 gcaaaattac tgaaggtcaa caagaagtct aaatttcgaa ttgtgccatc ttgtactatt
     1801 tataatgaat gcggaggctg ccaaatcatg cacctgcatt atgataagca gctggagttc
     1861 aagacggact tacttcatca agcgctgaaa aaatttgctc ctgcaggata tgaaaattat
     1921 gaaattcgtc caactattgg aatgcaggaa ccaaaatatt acagagctaa gttacaattt
     1981 cagactcgaa aatttaaaaa tcaggtcaag gcgggcttat atgcacaaaa ctctcactat
     2041 ttagtagagt tgaaagactg cctggtacaa gataaggaaa cccaagtgat tgctaatcgc
     2101 ttagcagaat tacttactta tcaccagatt ccaatcacgg atgagagaaa agttctaggt
     2161 gtccgtacta ttatggtccg acgcgcgaga aagaccggac aggttcagat tattattgtt
     2221 acaaaccgcc agcttaattt aactcaattg gtaaaagagt tggttaaaga tttcccagaa
     2281 gttgtgacag tagctgttaa tacaaataca gctaaaacca gtgagatata tggtgaaaag
     2341 acagagatta tctgggggca agagagtatt caagaaggtg tactcaatta tgaattttca
     2401 ctatcccctc gagcttttta tcaactaaat cctgagcaaa cagaagtcct ctatagcgaa
     2461 gcagtaaaag cgctggatgt tgataaagaa gaccatttga ttgacgctta ttgtggagtt
     2521 ggaacgattg gatttgcctt tgcaaagaaa gttaaaacac tcagaggtat ggatattatt
     2581 ccagaagcta ttgaagatgc caagcgaaat gctaaaagaa tgggatttga caatactcat
     2641 tatgaagctg gaacggcaga agagattatt cctcgttggt acaaggaagg ctaccgagca
     2701 gatgctttga ttgttgaccc accacgtaca ggtctggatg ataagttatt agatactatt
     2761 cttacttatg taccagaaaa aatggtttat atttcttgta atgtttcgac cttggctcgt
     2821 gatttggtac gcttagtaga agtctatgat cttcattata tccagtcggt cgatatgttc
     2881 ccacatacag ctcgaactga agctgttgta aaattaataa aaaaagttta aaaaagtagt
     2941 tgacaaagtt tgaaaaggct gtataatagt aagagttgaa aataacaact caggtccgtt
     3001 ggtcaagggg ttaagacacc gccttttcac ggcggtaaca cgggttcgaa tcccgtacgg
     3061 actatggtat gttgcgtcag ga
//
LOCUS       DAVLVF010000131         2862 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVLVF010000131 DAVLVF010000000
VERSION     DAVLVF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.131"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..496)
                     /locus_tag="VYN66_001916"
     CDS             complement(<1..496)
                     /locus_tag="VYN66_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1495888.1"
                     /translation="MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIIVPYTNLGEQQRIASELDLLSKLILRRQE
                     QLEEL"
     gene            complement(530..1495)
                     /locus_tag="VYN66_001917"
     CDS             complement(530..1495)
                     /locus_tag="VYN66_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1495889.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(1613..2026)
                     /locus_tag="VYN66_001918"
     CDS             complement(1613..2026)
                     /locus_tag="VYN66_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV1495890.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(2023..2259)
                     /locus_tag="VYN66_001919"
     CDS             complement(2023..2259)
                     /locus_tag="VYN66_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEV1495891.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            complement(2319..2855)
                     /locus_tag="VYN66_001920"
     CDS             complement(2319..2855)
                     /locus_tag="VYN66_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1495892.1"
                     /translation="MFGDPLNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAYGG
                     NGIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKVWISDNAIYIKEFKNSDFNLVFL
                     LELMKVIDFSKFADFSGQPKITQKPLENQKYILPPLALQNEFADFVALVDKSQLAIQK
                     SLEELETLKKSLMQEYFG"
BASE COUNT          855 a          562 c          410 g         1035 t
ORIGIN      
        1 taagctcttc aagttgttct tgtctcctta aaatcagctt gcttaacaaa tccagctcac
       61 ttgcaattct ttgttgctct cccaaatttg tatatggaac tataatatta tcaaaatatt
      121 ttttagttaa atgtttcatt gtggaaccat gagtatgttt tacagcatct tgtaaacctt
      181 tttcaacaac atatttaaaa tacgatttat ctatatctat cttatcaaaa accactttaa
      241 aaatgtgctg atttaatact gcggatcttc cacaccactg aaaaacccct aatgtacccg
      301 accacgaaat aagaatatca ccagcttcaa caatgtattt tttatcaatc gtacccgaat
      361 aataatttat cccttttgaa gttttagtca gattctgtat tctgataatc tctttccctt
      421 cagatgacca atcttgaggt ttaaaagcat agccattgat aaaagtagcc acttgcccta
      481 atttcacttt cttcatgcaa ccacctcctc ggaatatcgg gatttgcttt taagagatga
      541 atttttggtg tgaatgcttg acattatttt gattgaccat ggcataggcc agggttgtgt
      601 caatcttgct atgacctagc agtttttgga cttgttcgat aggcatacct ttatcaattg
      661 ccttagtcgc taaagttctt ctgaacttat gtgggtgaac cttttgtatg ccgagtttgt
      721 ctcctaactt tcttaagcga atctctacac cagcaattcc aagtctctgg gcttttccaa
      781 ctagcgttac aaaaagagca gggtgactat cttttctgtc gttaagataa tttcttaaat
      841 gaattttcgt ccgagcgtca aaatatactg gtctctcctt ctttccttta ccaaagacaa
      901 cacactctct gttttcaaaa tcaatatctg aacgattcaa ctgtacaagc tcccctacac
      961 gcatacctgt cgatgctagt aggtctatta ttgccaaatc tctcaaattt tcacagttat
     1021 cacgcataat ttccaaatgt tcatcagtat aagtttcctt cacattttgc tcagtcttaa
     1081 ttttctttat ccgtcgaatg ggatttttaa tgatatattc ctcttgctca agccaagcaa
     1141 aaaaagaaga caatatacgc ctaatattgt ctaaatttgc cttactacaa tccttttcag
     1201 actggtaatt tgctaaataa ctccttaaat catctgttgt gagttgtgtc acagactctt
     1261 taatagcatt aaacaagttt tcaatcgtcc tctggtaata acgaattgtt tttgagctac
     1321 agccctctac ctgcttggcg gtaataaata gttgcaacag gttattattc gaatacttct
     1381 gctgcttttt tccgtctact agatgatgtt ctaaactctc tctcaacgct agtaattgtt
     1441 cattatttaa aagaggtaac atttcttgca tgataattgt aacttttgat tccataattt
     1501 ctcactttct tattcatcaa taaaaagaga caatatcagt ttctgcatta cagaattcta
     1561 atattgtctg ttcatttatt ttcttgtgct cattcaaatc cagcatacta ggttactttt
     1621 tgaccttttc tctaacagaa tgttcagaaa cccatttgga gtagcttgtc attttttcat
     1681 cagttaaagc ttctactgcg atggttacac acatttttac tgcttcttct accgttacag
     1741 aaaaacgata gccgtttaat tgtaaaaatt tgaccaaaac gaagaaagca gttcttttat
     1801 tagcattagc aaaaacatgc ttctttatca attggacaaa tagtatcgtt gctttatcaa
     1861 aaattgttgg ataaagagac ttcccaaaga caaattgttc tggtaagttc acaatcatat
     1921 ttaaggcaga agaactaact gtttgaattt tctcatttgg agaataccgt tgaattgcta
     1981 aagcatttat tttttcaatt tgcttttctg tcagatagat tgtcattttt ccaccaaagc
     2041 tttgaaaaca tcgtcgtatt cgtcaaatat tttatctaaa tttaagtcaa aggcatcatc
     2101 agatacataa gaaacctgct gccccaattc ttcaggagta aagataatct caccacttgg
     2161 taacaatttc gcttcgtatt taacaccact tggaatatta aattcacttg gaatcgtaat
     2221 ggtgattgaa tttccttgtt ttcttgtttt tactaccatc tgatttcctc ctttgaagta
     2281 ttataacaaa tcattaccgt aattacaatg gcagaatatc agccaaaata ctcctgcatc
     2341 agagatttct tcaaagtttc aagttcttcc agagattttt ggattgccaa ttgtgatttg
     2401 tcgaccaggg ctacaaagtc tgcgaactca ttttggaggg ctagaggggg gaggatatat
     2461 ttctgatttt ctaatggttt ttgagttatc tttggttgac ctgagaaatc agcaaattta
     2521 ctaaaatcaa ttactttcat taattctaat aaaaatacaa gattgaaatc ggaattctta
     2581 aattctttta tatatatagc attgtcactt atccatacct taccgtgggt agttcttaca
     2641 ttaccacaat atgcacctac acgaccaatg atgatagtag gattatcaat taaatatttt
     2701 cgacttttcc aagcaatgcc atttccacca taggcaggat aaccttcaaa aactctatca
     2761 tgtttatcaa gaaattttcc acttgaaaat ttaaaacact gttgtcccgt ttttactgca
     2821 aatttcttgt tattatttaa aggatcccca aacatctcgt ta
//
LOCUS       DAVLVF010000132         2832 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVLVF010000132 DAVLVF010000000
VERSION     DAVLVF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.132"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(1..688)
                     /locus_tag="VYN66_001921"
                     /pseudo
     CDS             complement(1..688)
                     /locus_tag="VYN66_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(781..939)
                     /locus_tag="VYN66_001922"
     CDS             complement(781..939)
                     /locus_tag="VYN66_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEV1495893.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            complement(1010..1439)
                     /locus_tag="VYN66_001923"
                     /pseudo
     CDS             complement(1010..1439)
                     /locus_tag="VYN66_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1534..1974)
                     /locus_tag="VYN66_001924"
     CDS             complement(1534..1974)
                     /locus_tag="VYN66_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1495894.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNWHIKASQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHDQCRSGSVVQEFLGDYSGYVHCDILRQ"
     gene            1965..2273
                     /locus_tag="VYN66_001925"
                     /pseudo
     CDS             1965..2273
                     /locus_tag="VYN66_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2428..2751)
                     /gene="tnpB"
                     /locus_tag="VYN66_001926"
     CDS             complement(2428..2751)
                     /gene="tnpB"
                     /locus_tag="VYN66_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1495895.1"
                     /translation="MTIHLSSLGEVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLIWLSTEKDVKALTPEQVDWLMKGFS
                     ITPKI"
BASE COUNT          839 a          590 c          531 g          872 t
ORIGIN      
        1 gatgttctca accttgcttc tctccttaga tagcgcatgg ttatagactt tatcttcaat
       61 tgttagcggt ttgagtttgc tggatttacg tgaagtttgt gcttgaggat atatcttcat
      121 gagcccttga taaccactgt cagccaagat tttaccagct tgtctgatat ttctgcgact
      181 cattttgaac aacttcatat catgacaata gttcacagtg atatccaaag aaacaattct
      241 cccttgactt gtgacaatcg cttgagcctt catagcgtga aatttctttt taccagaata
      301 attcgctaat tcttttttta gggcgattga tttttacttc cgtcgcatca atcattaccg
      361 tgtcctcaga actgagagga gttcttgaaa tcgtaacacc actttgaaca agagttactt
      421 caacccattg gctccgacgg attaagttgc tttcgtgaat accaaaaaca gccgcaattt
      481 gttcataagt tcgatattct cgcacatatt gaagagtggc cataagaagg tcttctagac
      541 ttaatttagg ttttcgtcca ccttttgcgt gtttaagttg ataagctgtt tttaatacag
      601 ctaacatctc ttcaaaagtc gtgcgttgaa caccaacaag acgcttaaat cgtgcatcgg
      661 ttagttgttt acttgcttca tcattcatag aactactata ccatattttg tttcgcagga
      721 agtctattga aaaatctcca gactggagaa ctcacggata gttcctaatc tggagatttc
      781 ttatttgcac ttttcttgta caactttagt ccatggtaaa taagcctcta aaacctcttt
      841 gtttacgaga gtttcctcgt ttggaagaca ttctagaaga taggatagat atttctcgct
      901 atttaattga tgacgtttag ctgtttccaa caaactcata ataatagctc ttgctttagc
      961 tccttcaaaa ctttgagaaa ataaccaccc tcgctttttt aaactgagct taggctaaaa
     1021 gagtccactg gactctttta ctccgtccca taaccaatga tttaatggcg cgttcagcta
     1081 gattattgga aaggaccaga tgtccgtctt tcaaaatggt cttaaaggtt tcttcatact
     1141 tgaggctgta ttcaattgcc cttcctagtt ttgaacctgc taaaactgac tgacgccggc
     1201 actaagcaaa gaagtcttcc attaagggct ggagatgttc ttgacgtttc tgtagtcgtt
     1261 catcagctgg caaagcctcc cagtttcttt ccaaggaaaa taactgatca caataagcta
     1321 aacctttagc tcctaaggat gatttatccg cttgcttggg ggggcgcttc aaaaaacttc
     1381 cttctcacat gcgcccaaca tccaacaagt tcagcttctt ccagttgacg ataagcagac
     1441 gagcggttcg aagtttacca agcttagcgt cgccttgctc ctaaaccttg gactgctatc
     1501 gcataggcag aactagagga ctaaagtcct aagttactgc cgcaaaatat cacaatgcac
     1561 atagccagaa taatctccta ggaattcttg tactactgaa ccacttcgac actgatcatg
     1621 gtggtaaagc gtaatccctt gtttctctgc tttacctgac aaaaaagtcc aatagtaagt
     1681 cagctgacta tcactctcta gcaccctata agaagtttca tccgcatgaa gtaagggctg
     1741 agtcaatagt ctctctcgca agaggttata aaggggctcc aaatagtatt gactcgcctt
     1801 gatatgccaa ttagagattt ccttacgtgt gattggtaaa cccatcctag cccaatcttc
     1861 ttcttggcga taattgggta ccttcagatt aaacttctga tggatggtgt gagcgataat
     1921 agaagctgag ccaaagttat gcgctaaagg ggctttagga ataggagatt tcacaagctt
     1981 atccagatga ttatctttta ctcgttatgg acaatgctat atggcataaa tcaagtacct
     2041 taaagattcc gactaatatt ggctttgcat ttattcctcc atacacacca gagatgaacc
     2101 ccattgaaca agtgtggaaa gagattcgta aacgtggatt taagaataaa gccttttaaa
     2161 ctttggaaga tgtcatgaat caactccaag atgttataca aggactggag aaggaggtga
     2221 taaagtccat cgttaatcgg agatggacta gaatgctttt tgaaagcaga tgagtataaa
     2281 aagaaagtcc tcatttcaat agaaatcacg actttctgat ggatttatag taaaatgaaa
     2341 taagaacagg acaaatcgat caggacagta aaatcgattt ctaacaatgt tttagaagca
     2401 gaggtgtact attctagttt caatctacta tatttttgga gtgatagaaa agcccttcat
     2461 aagccagtct acttgttctg gtgtgagagc tttgacatcc ttttctgtac ttagccaaat
     2521 caatctgccg ttctcaaagc gtttatatag tagccaaaat ccttgaccat cccagtaaag
     2581 gactttaaag cggtctttac gtccaccaca aaagagaaag acttgaccgg agaaaggatc
     2641 caattcaaag tgggttttaa ccagataagc cagtgaatcg attccttgtc tcatatcagt
     2701 tttcccacac acgagataga cctcccctag gctagataga tgaattgtca tagagcaaca
     2761 ccttatccaa aagctgttct accatttcga gattgagaga ttgaaaaaaa cttacttcaa
     2821 gcttgccaat ac
//
LOCUS       DAVLVF010000133         2807 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVLVF010000133 DAVLVF010000000
VERSION     DAVLVF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.133"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..885
                     /locus_tag="VYN66_001927"
     CDS             <1..885
                     /locus_tag="VYN66_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1495896.1"
                     /translation="LAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDY
                     SLKKFDIDLKDVEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYD
                     YDKGQILIDGKDIKEISTESLFDKVSIVFQDVVLFNQSIMENIRIGKQDASDEEVKRA
                     AKLANCTDFIEKMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASL
                     DVNNEKKIQESLNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKS
                     KIYKNLIEKTKMAEEFIY"
     gene            896..1480
                     /locus_tag="VYN66_001928"
     CDS             896..1480
                     /locus_tag="VYN66_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1495897.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            1480..2175
                     /locus_tag="VYN66_001929"
     CDS             1480..2175
                     /locus_tag="VYN66_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1495898.1"
                     /translation="MSTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGISF
                     KNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            2163..>2807
                     /locus_tag="VYN66_001930"
     CDS             2163..>2807
                     /locus_tag="VYN66_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495899.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSINVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKG"
BASE COUNT         1010 a          361 c          541 g          895 t
ORIGIN      
        1 ctagctgcta tgaagataaa agactcttta gatgcatcta aagagggctt gatggaaata
       61 ttttatttat cgcccaaaat agaaagatta aaagaaattc aaaatcaaga tttacaagaa
      121 ggcgatgact atagcttaaa aaaatttgat attgatctaa aagatgttga gtttgcctac
      181 aataaagacg caaaagtttt aaatggtgta agttttaaag ctaagcaggg agaggtcact
      241 gctttggtag gtgcaagtgg ctgcggtaaa acaactatct tgaaacttat atcaagactt
      301 tatgattatg acaagggaca aatcttaatc gatggcaaag atataaaaga aatatcaaca
      361 gaatctcttt ttgataaggt ctctatagtt ttccaagatg tggttctctt taatcaaagc
      421 attatggaaa atattagaat cggaaagcaa gatgcaagtg acgaagaggt taaaagagca
      481 gcaaaacttg caaattgcac agattttata gaaaaaatgg ataaaggttt cgatacagtt
      541 attggtgaga acggagctga gctatcagga ggagaaagac aaagattatc aatagccaga
      601 gccttcttaa aagatgcgcc gatattgatc ttagatgaga taacagcaag ccttgatgtt
      661 aacaacgaga aaaagattca agagtcttta aataatttag ttaaagataa aactgttgta
      721 atcatttcac atagaatgaa atccatagaa aatgcagaca agatagtagt tcttcaaaac
      781 ggaagagtag aaagcgaagg taagcatgaa gagcttttac aaaaatcaaa aatttacaaa
      841 aatttaatag aaaagacaaa aatggcagaa gaatttattt attaggagga ctacaatgga
      901 taataaaaaa ttaaaagtaa aagatttagt aagtatcggt gtttttggcg taatttattt
      961 tgccttcatg tttggagttg gtatgatggg cttgattcca atattgttct taatataccc
     1021 gacagtatta gccatagttg caggaactgt tgttatgtta tttatggcta aggttcaaaa
     1081 gccatgggca ctatttatat ttggtatgat atcaccactt gtgatgtttg cagctggtca
     1141 tacctacgta gttgtggttt tatcacttat agtaatgata atagcagaat taattagaaa
     1201 gattggtaat tataattcat ttaaatacaa tatgctttct tatgcaatct tcagcacatg
     1261 gatatgtagc tctttaatgc aaatgctttt agcaaaagaa aaatatatgg agtggtcttt
     1321 gatgacgatg ggaaaagatt atgttgatgt attagaaaag ttaataactt atcctcacat
     1381 ggctttagta gccttaggtg ctttcttagg aggaattctt ggagcatata taggcaaggc
     1441 tctattgaaa aaacactttg aaaaagcagg cattgtataa tgtctacttt ttcttcaaat
     1501 gctcccttta atcccaatcc gatctctaag atattagtcg tatttatcac aggcttaact
     1561 gttatgcata gcataaacat ccgttttgag ctggcaattg tttgtcttat tggtattttg
     1621 ctttatttga atggatacaa aaaaacactt ttcaaatgga ttatattgtg tgggatactt
     1681 tactccttgc ctaattttat ggtgttatct gaattaaacc ctatagttaa gatgtttttg
     1741 agtataccta ttgttattag aatgtttatt ttaccattta tggcagcaag ctttatgata
     1801 aagacctcgg atgtaggcgc aataatttca tcgatggata agcttaagat ttcaaagaat
     1861 gtatccatac ctattgcggt tatgtttaga ttcttcccat cttttaagga ggagaagaaa
     1921 aacatcaaaa tggctatgag agtaagaggg ataagtttta aaaacccagt caaatatctt
     1981 gaatatgttt ctatgccact actcattata tcatctaata tatcagatga cattgcaaaa
     2041 gcggcagaaa caaaggcaat agaaaatcca attgccaaga ccagatacat tcgcgtaaag
     2101 atacagctaa ttgattttgt ttatgtttta gcggttgctg gacttattgt gggaggctta
     2161 atatggttga aataaaaaat ttaagtcttg attatggtga agagcatata ttagatgata
     2221 tatcactatc catagccgag ggagagtgcg tgctatttac aggaaaaagt ggaaatggta
     2281 agtcatcttt aataaattca atcaatggac tagctgtaag gtatgataac gcaaagacaa
     2341 agggcgaaat aattattgat ggtaagaata taaaaaattt ggaactttat caaatctcaa
     2401 tgcttgtttc aactgttttt caaaatccta agacatattt ttttaatgtc aatacgacat
     2461 tagaattatt attttatttg gaaaatatcg gtcttgcaag agaagagatg gacaggcgtt
     2521 tgaaggatat gcttaagata ttcccgataa aaaatctttt gaacagaaat atatttaatc
     2581 tatccggcgg tgaaaaacaa attctttgca ttgcagcttc ttatatagca ggtacaaaga
     2641 ttatagttat ggatgagcct tcatcgaatt tagatattaa aagcataaat gttttggcaa
     2701 agatgctaaa gatattaaaa gagaaaggca taagcataat tgttgcagag catagaattt
     2761 attatttgat ggacatagtt gaccgtgtat ttttaataga taaagga
//
LOCUS       DAVLVF010000134         2732 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVLVF010000134 DAVLVF010000000
VERSION     DAVLVF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.134"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(272..829)
                     /gene="sdbB"
                     /locus_tag="VYN66_001931"
     CDS             complement(272..829)
                     /gene="sdbB"
                     /locus_tag="VYN66_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV1495900.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPNFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKIVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            complement(840..1547)
                     /gene="ccdA2"
                     /locus_tag="VYN66_001932"
     CDS             complement(840..1547)
                     /gene="ccdA2"
                     /locus_tag="VYN66_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1495901.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            complement(1778..2183)
                     /locus_tag="VYN66_001933"
                     /pseudo
     CDS             complement(1778..2183)
                     /locus_tag="VYN66_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2225..2425)
                     /locus_tag="VYN66_001934"
     CDS             complement(2225..2425)
                     /locus_tag="VYN66_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495902.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            complement(2592..2732)
                     /locus_tag="VYN66_001935"
                     /pseudo
     CDS             complement(2592..2732)
                     /locus_tag="VYN66_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          800 a          573 c          497 g          862 t
ORIGIN      
        1 tctcaaagta acttccatct ctctcccaaa actggaagtt actctaagac gactctgttt
       61 tagggctatt tgtcgtgcac tttttctgag tcttttcgct tttgaaccct tatatatcaa
      121 gaaaaagaaa accagtggaa gttagtctaa gacggatttc cactgatgtc acgcgttttt
      181 ctcatactaa aagctaggtt gtgggaaact ggaagttagt tttagaagtt accaaaatct
      241 gactacatct tgtaatcaga ttttatcgat tctagttcat ttctttaaat gctgcttctg
      301 catccgcatt actgatagca ccaaattgaa tctttccaat ctttccttgg ctatcaatta
      361 aatattctgt aggaatgctt cgaatttgat aagcttggaa ggtggttgct ttggtatcat
      421 aaagaactgg gatatcctta tatccttgtt cctggaacca ttgtgggaat tgctcaacaa
      481 ttttttcacc ttgaattcct ggtgcaatga cagtaagaat ttcgaaatca cgatctggtt
      541 tcgccgctag ttccatcaac tctggcatac ttttcttgca tggaccacac catgaagccc
      601 aaaacttcaa gtaaaccttt ttacccttaa aatcagataa cttaacttct ttgccatcca
      661 tggattgcaa tgtgaagttt ggagcatctt ttccaacagc aatttgttgt acagtcgttt
      721 gttgttttgg ctgttgtgct gcttgagtct ttttagtttc ttcctcacca caggccatca
      781 atacaactaa tgacaagaga cttaagccag caaacattac ttttttcatt tgtcctcctt
      841 tattcaaaaa ttccagctag aacatttact tgtcctaata gtaacaaaat tcccattaaa
      901 acaatgagga aaccaccaat tttctttagt agcatcatat gacgcttgat tttactaaaa
      961 tatggcatga ctagacctga agctagtgcc aataccaaga aaggaagggc catgcccaga
     1021 gtgtaaatga gagtataaat cgctccttgc caagcgccat tgcctccaga agccgcaagt
     1081 gctaaaacag aacttaaaac tggaccaata caaggtgtcc aaccaaagct aaaggtaata
     1141 ccaagtaaaa aagctgacca ataacgatta gagtctgatt ttttaaaggt aaaacttttt
     1201 tgaacttcta atttcttcaa atgaaaaatt tccatctggt gaagacccaa aatgataata
     1261 atagctccca tgccatatcg aaaccaattt gcatagagaa tatgaccaaa gtaaccagca
     1321 ccaaagccta gaataaagaa aatgagagag ataccagcga taaagcaaag tgttcgaatc
     1381 aagcctgacc agagaacctt tctcccaaac aaagaaaagc tttttgcact ttcttgatca
     1441 tccaataaaa tcccagcata gactggcaga agaggaaaaa tacaaggaga aaaaaaggat
     1501 aaaacacctg ctagaaaaac agagattaaa aatactatcg tttccaataa agaaccaact
     1561 ttcttaataa ttctaatcct attttactat attcaatttt atttgtaagc tttctgctac
     1621 gcaaaatcgt atcgggcact attggaccaa tcttttcttt tgctagtcaa ggcggatctt
     1681 atcccccaaa atagccaaaa agcaacgaca aggattactc gtcgctgctt ttgtgaacga
     1741 aaatgtcttt taggtctgac atttcataaa tcatgtttta cttgagtttg tcaaggattg
     1801 ctttaagctc ctctactagt ttagtttctg tctctgctga gccattttct tctttcacga
     1861 aatcaagggt ttcttggaga aggttttggg ctttggcaag gactttttta tccgcttttt
     1921 tctgcatcta gctgtcctag aaccttggtc aattccgtgc ttaattgctg gatttctgac
     1981 tctttcttac ggcgaattag ccagaaggca atcacgccta ggagggcaag tagactgacc
     2041 acaatcactc ctgctggaac tgagtttgtt tcagtcatct tatctgaatc cttactatct
     2101 tccgttcctt gttttgcatc cttcttgtcc tgtgtaggct tgctgtcgct agcatttgct
     2161 ttcacatctt tgagagagtc caaggcagcc cagccttcac agactctact gcagtatgca
     2221 gaccttactc tgtcaaggca ctatcttccg gagctttttg agcatctagg aggacagcct
     2281 tggttgcatc gattttcgga tcagatactg ttgccaaagc tttcaagcgt tggtctaact
     2341 cttgactcaa ggcacgaagt tcagacttgt caacttgctc ttgagcttgt gtgctcgttg
     2401 agctagccga agcgcttgct accactctag gatcttgagt cggagctgag cttggagctg
     2461 ggacagggct tgcaggttga ctaggaacag ttatggtata ttgaaactag aatagtacat
     2521 atggacttct aaaacattgt tagaattcga ttttactgtc ctgatcgatt tgtcctgttc
     2581 ttatttcatt ttactataat aaccgatggt gtggttaatg ttggtaagag aaacttctga
     2641 aaccatactt caaaaaagtc gctcgtcatc atctcttcgt aagtcattgg agcgattaat
     2701 tcaccatttg ttagacctgc aaccaaagaa at
//
LOCUS       DAVLVF010000135         2653 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVLVF010000135 DAVLVF010000000
VERSION     DAVLVF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.135"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            34..105
                     /locus_tag="VYN66_001936"
     tRNA            34..105
                     /locus_tag="VYN66_001936"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:66..68,aa:Gln,seq:ttg)
     gene            267..1469
                     /gene="rpsA"
                     /locus_tag="VYN66_001937"
     CDS             267..1469
                     /gene="rpsA"
                     /locus_tag="VYN66_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV1495903.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1554..2653)
                     /locus_tag="VYN66_001938"
                     /pseudo
     CDS             complement(1554..2653)
                     /locus_tag="VYN66_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          808 a          443 c          593 g          809 t
ORIGIN      
        1 ggggttcgaa tccctctctc tccatttcat taatggggta tagccaagcg gtaaggcaag
       61 ggactttgac tccctcatgc gttggttcga atccagctac cccagttctt aggtaataat
      121 caagataaaa agcaaaatat cttagggtat tttattttta taattgaaag acgtgaatga
      181 tatgaacatg tccttgcggg tgcttaggaa aaaaattata agtatgtcaa gtttaagaaa
      241 aacttgattg ttggaggatt ttttagatga acgaatttga agatttgcta aatagcgtta
      301 gtcaagttga gactggtgat gttgttagtg ctgaagtatt gacagttgat gcgactcaag
      361 ctaacgttgc aatctctgga actggtgttg aaggtgtctt gactcttcgc gaattgacaa
      421 acgatcgtga tgcagatatc aatgactttg ttaaagtagg agaagtattg gatgttcttg
      481 tacttcgtca agtagttggt aaagatactg atacagttac ataccttgta tctaaaaaac
      541 gccttgaagc tcgcaaagca tgggacaaac ttgttggtcg cgaagaagaa gttgttactg
      601 ttaaaggaac gcgtgccgtt aaaggtggac tttcagtaga atttgaaggt gttcgtggat
      661 ttatcccagc ttcaatgttg gatactcgtt tcgtacgtaa cgctgagcgt tttgtaggtc
      721 aagaatttga tactaaaatc aaagaagtta acgctaaaga aaaccgcttc atcctttcac
      781 gtcgtgaagt tgttgaagca gctactgcag cagctcgcgc tgaagtattc ggtaaattgg
      841 ctgttggtga tgttgtaact ggtaaagttg ctcgtatcac aagcttcggc gctttcatcg
      901 accttggtgg tgttgacgga ttggttcact tgactgaatt gtcacatgaa cgtaacgtat
      961 caccaaaatc agttgtaact gttggtgaag aaattgaagt gaaaatcctt gatcttaacg
     1021 aagaagaagg acgtgtatca ctttcactta aagcaacagt accaggacca tgggatggcg
     1081 ttgagcaaaa attggctaaa ggtgatgtag tagaaggaac agttaaacgt ttgactgact
     1141 tcggtgcatt tgttgaagta ttgccaggta tcgatggact tgttcacgta tcacaaattt
     1201 cacacaaacg gattgaaaat ccaaaagaag ctcttaaagt tggtcaagaa gttcaagtta
     1261 aagttcttga agttaacgca gatgcagaac gcgtatcact ttctattaaa gctcttgaag
     1321 aacgtccagc ccaagaagaa ggacaaaaag aagaaaaacg tgctgctcgt ccacgtcgtc
     1381 caagacgtca agaaaagcgt gatttcgaac ttccagaaac acaaacagga ttttcaatgg
     1441 ctgatttgtt tggtgatatc gaactttaat caaattgaaa attcacaaaa tcctttgttt
     1501 actaaacaag ggatttttct ggctctttgt caactgtagt gggttgaagt cagctaaggt
     1561 tgagaaagga caaatttcgt cctttctttt ttgatgttca gagcgataaa aatccgtttt
     1621 ttgaagtttt caaagttccg aaaaccaaag gcattgcgtt tgataagttt gatgagatta
     1681 ttggtcgctt ccaatttggc gtttgaatag ggtagttgaa gggcgttgac gattttctct
     1741 ttgttcttta gaaaggtttt aaagacagtc tgaaaaagag gatgaacctg cttaagattg
     1801 tcctcaataa gtccgaaaaa tttctctggt tccttattat agagctgata gtggtgtttc
     1861 aagtcttctg aatagctcaa aagcttgttt aaaatctctt tattggttaa gtacatacga
     1921 aaagtagaac gataaaatcg cttatcactc agtttacggc tatcctgttg aatgagtttc
     1981 cagtagcgct tgatagcctt gtattcatga gactttcgat gaaactgatt catgatttgg
     2041 acatgcacac gactcatagc acggctaaga tgttgtacaa tatgaaagcg atcaagaacg
     2101 attttagcat tcgggagtga aacagtctga gagactgttt cagcctgagc ctagaaattt
     2161 gaaagcgaag ttgtctagct aagtcatagt aaggactaaa catatccata gtaatgattt
     2221 tcacttgaca acgaacggct ctatcatatt taagaaagtg atctcggatg atagcttgtg
     2281 ttctaccttc aagaacagtg atgatattga gcttttcaaa atcttgcgca ataaagctca
     2341 tctccatctc ccgattgaaa cagtcactcc ccggactgtt tcaacgtccc aagacataat
     2401 ctcaggcaga tttctaaaat tacactcaaa gtggaagtca ttgagctttc gaatgacagt
     2461 tgaagttgag atggaaagct gatgggcaat atcggtcata gaaatctttt caattaactt
     2521 ttgagcaatc ttttggttga tgatacgagg gatttggtga ttcttcttga cgatagaagt
     2581 ttcagcgacc atcatttttg aacagtgata gcacttgaat cgacgctttc taaggagaat
     2641 tctagtaggc ata
//
LOCUS       DAVLVF010000136         2638 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVLVF010000136 DAVLVF010000000
VERSION     DAVLVF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.136"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            31..>2638
                     /locus_tag="VYN66_001939"
     CDS             31..>2638
                     /locus_tag="VYN66_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEV1495904.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKSMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
BASE COUNT          931 a          319 c          536 g          852 t
ORIGIN      
        1 agtttgataa aatttcttgg agattttgtt atggattata atttcaattt ggagcatcct
       61 ttctttttca ctaataatga ttatagtaca gatactagca taaagtacca agtaagtctg
      121 ccttttaatt ggcacgaagt aatgaataat gatgagtggg tttatcaata ccctattgga
      181 aaatttgttg aaagacaagg gtggaaaata cacattagtt cagagtataa cagttcacat
      241 gaattgttac aagatgtagc aaaaatttgc catgaaatgc gaattccatt taaacatctt
      301 agtacagaag acaaatttat catgcgaaac gggaaattgg ttagtcgagg attttctggt
      361 aaatttatta catgttatcc aaatcaaaat gaattagagt cggttttgca aaggttagaa
      421 agtgccttaa agcaatataa tgggccatat attttaagcg ataaacgttg ggatgaagca
      481 ccaatatatc tgaggtatgg tgtgtttagg ccgtcaaggg atgatgaaaa gaaggttgct
      541 attgatgaat taattgttgg cgatgaagtt gttaaagatg aacgtttacc agtatttaaa
      601 attccaaaag gtatagtgcc ccctgacttt ttgaataaat ggcttgacaa gaaagataaa
      661 aaacagggag actttccctt tattatagat aatgcaatta gattttcaaa cagtggtggg
      721 atatataatg ctaggctaaa agaagatggg aaaaaaataa ttttaaaaga ggctagaccg
      781 tatacaggtc taggatttga tggtacttat tcttcagaga aattagcttc ggagtgcaaa
      841 gcactaaaaa ttctaaatga atggagtgag acgcctaaaa tatactggca cggtaagatc
      901 tgggagcaca ccttcttagg aattgaacat atgaagggcg ttccactaaa tagatgggtt
      961 accaataatt ttcctttata tgaagttgtt gataagacca aagattatct attgagagtt
     1021 tcaaagatag tagaaaaact catcgattta acgaataagt ttcattctga aaatgtatat
     1081 catcaagatt tacatttagg aaatatcttg gttaaggatg aagatgagat tagtataata
     1141 gactgggaac aggctgtctt ttcaaatgat gaaaaagttg tacataaagt tgcagcccct
     1201 ggttttagag catggcgaga aacacttccc agtgagatag attggtatgg tattagacaa
     1261 atagctcatt atttatacat gccactagta actacatcgg atttgacata caactatgta
     1321 agtcaaacca ggatagaagg aaaaaaacta tttgagtctt tgggttatac aagagaacat
     1381 atagattatg tagaaagttt attgtcatat ctagattcta aatgtccaca gattgagaat
     1441 atttccagga aaaaagtttt aaaatccatg catgagatta gaactatcga gagtgagcaa
     1501 gatatacaag attttattat aaaattattg agagggttta cgctaactta tggacagtgg
     1561 cgtaaagaat ttcaatcaag attcttccca gttcattatt atggtctgaa ttttaatcaa
     1621 ggaattgcgt tttcagattt agctatcctt tggagttatc aacaactagc taagaaagta
     1681 aaaaatttca agtttgatga ctattatgag attcgaacac aggttattaa tgaggcagta
     1741 aataatttta agaaatcgtc cctttctggt ctttttgatg gtaaaattgg tacaatctgg
     1801 ctcatttatg aatttgggga gatagatcgg gcagttgaac tttttacaac tcattttata
     1861 gagatatttg agaattcaca aaataaaaat ttatatagtg gacaggctgg aattttactg
     1921 gttggactgt attttcttag taaaggggaa attgataata aactaggaga agaaatacta
     1981 attaggctta gagaatatac tttaaattat atagaaaatc cagaaacttt ttgtaaagtt
     2041 ggcgctagtg atgttcagag taatgatcct tatgagaatt ttggtggatt actatatggt
     2101 cacgcagggg tagcttggct cttcggtgaa gcttataaat taactggtga aagtatctat
     2161 aaaaatggtc tagaattggc tgtagataaa gaattagttg cttataaagt agatagtaat
     2221 aattcattgc agtacagcca aggtcataga ttactaccat atctagcaac tggaagtgct
     2281 ggcctgcttt tacttattaa tagaaataaa gaaatcttgt caagcaaata cttaaaatat
     2341 ttaacttctt tagagagggc aactgatgta gttttttgtg tattaccagg tttatttaac
     2401 ggattttgtg gtcttgaagt tgctaataat atatatagtg atatcgatga taacttttca
     2461 ggacaaaaga aattaattga acaactttat agatatttgt gtgtaattga agaaggattt
     2521 gttattgcgg gagataatgg tttaaagata acaacagata ttgcttcggg atttgcgggt
     2581 gttgcaattg gattggtatc aataatggat aataaattga cgattcttcc tcaaatat
//
LOCUS       DAVLVF010000137         2584 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVLVF010000137 DAVLVF010000000
VERSION     DAVLVF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.137"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            144..1340
                     /gene="tuf"
                     /locus_tag="VYN66_001940"
     CDS             144..1340
                     /gene="tuf"
                     /locus_tag="VYN66_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1495905.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(1449..2584)
                     /locus_tag="VYN66_001941"
                     /pseudo
     CDS             complement(1449..2584)
                     /locus_tag="VYN66_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          792 a          505 c          547 g          740 t
ORIGIN      
        1 ttaaattttt accaagaata attcacaaaa acgttgtaaa acacttgcaa tttagctgaa
       61 atttgataaa atagtaagga aagttagact gtattgccta ctgtctatct ataaaatata
      121 ttttattgga ggcttttact caaatggcaa aagaaaaata cgatcgtagt aaaccacatg
      181 ttaacattgg tactatcgga cacgttgacc acggtaaaac taccctaact gcagctatca
      241 caactgtttt ggcacgtcgc ttgccttcat cagttaacca acctaaagac tatgcgtcta
      301 tcgatgctgc tccagaagaa cgcgaacgcg gtatcactat caacactgcg cacgttgagt
      361 acgaaactga aaaacgtcac tacgctcaca tcgacgctcc aggacacgcg gactacgtta
      421 aaaacatgat cactggtgct gctcaaatgg acggagctat ccttgtagta gcttcaactg
      481 acggaccaat gccacaaact cgtgagcaca tccttctttc acgtcaggtt ggtgttaaac
      541 accttatcgt cttcatgaac aaagttgact tggttgacga cgaagaattg cttgaattgg
      601 ttgaaatgga aatccgtgac ctattgtcag aatacgactt cccaggtgac gatcttccag
      661 ttatccaagg ttcagcactt aaagctcttg aaggtgactc taaatacgaa gacatcgtta
      721 tggaattgat gaacacagtt gatgagtata tcccagaacc agaacgtgac actgacaaac
      781 cattgcttct tccagtcgag gacgtattct caatcactgg acgtggtaca gttgcttcag
      841 gacgtatcga ccgtggtatc gttaaagtca acgacgaaat cgaaatcgtt ggtatcaaag
      901 aagaaactca aaaagcagtt gttactggtg ttgaaatgtt ccgtaaacaa cttgacgaag
      961 gtcttgctgg agataacgta ggtgtccttc ttcgtggtgt tcaacgtgat gaaatcgaac
     1021 gtggacaagt tatcgctaaa ccaggttcaa tcaacccaca cactaaattc aaaggtgaag
     1081 tctacatcct tactaaagaa gaaggtggac gtcacactcc attcttcaac aactaccgtc
     1141 cacaattcta cttccgtact actgacgtta caggttcaat cgaacttcca gcaggtactg
     1201 aaatggtaat gcctggtgat aacgtgacaa tcgacgttga gttgattcat ccaatcgccg
     1261 tagaacaagg tactacattc tctatccgtg agggtggacg tactgttggt tcaggtatgg
     1321 ttacagaaat cgaagcttaa ttcgatttag ttcccagaag aacaattatt taagtcagac
     1381 actaaaagaa tcttgcttgg caaggttctt ttttggttct ttgtcaactg tagtgggttg
     1441 aaaaaaaact aagctcgaga aaggacaaat ttcgtccttt ctttttgatg ttcagggcga
     1501 tgaaaatccg ttttttgaag ttttcaaagt tccgaaaacc aaaggcattg cgcttgataa
     1561 gtttgatgag attattggtc gcttccaatt tggcgtttga atagggtagt tgaagggcgt
     1621 tgacgatttt ctctttgtcc tttagaaagg ttttaaagac agtctgaaaa agaggatgaa
     1681 cctgcttaag attgtcctca atgagtccga aaaatttctc cggttcctta ttctgaaagt
     1741 gaaacagcaa gagttgatag agctgatagt ggtgtttcaa gtcttccgaa taactcaaaa
     1801 gcttgtttaa aatctcttta ttggttaagt gcatacgaaa agtaggacga taaaatcgct
     1861 tatcactcag tttacgacta tcctgttgta tgagcttcca gtagcgcttg atagccttgt
     1921 attcatgaga ctttcgatga aactgattca tgatttggac acgcacacga ctcatagtac
     1981 ggctaagatg ttgtacaatg tgaaagcgat caagaacgat tttagcattc gggagtgaaa
     2041 cagtctggga gactgtttca gcctgagcct agaaatttga aagcgaagct gtttagctaa
     2101 gtcatagtaa ggactaaaca tatccatagt aataattttg acgcgacatc ggacggctct
     2161 atcatattta agaaagtgat ctcggatgac agcttgtgtt ctaccctcaa gaacagtgat
     2221 gatattgaga ttgttaaaat cttgcgcaat gaagctcatc tccatctccc gattgaaaca
     2281 gtcactcccc ggactgtttc aacgtcccag gacataatct caggaagacg cgaaaaatca
     2341 tgctcaaagt gagaatcatt gagcttgcga atgacagttg aagttgagat ggaaagctga
     2401 tgggcaatat cagtcataga aatcttttca attaactttt gagcaatctt ttggttgatg
     2461 atacgaggga tttggtgatt cttcttgacg atagaagttt cagcgaccat catttttgaa
     2521 cagtgatagc acttgaatcg acgctttcta aggagaattc tagtaggcat accagttgtt
     2581 tcga
//
LOCUS       DAVLVF010000138         2188 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVLVF010000138 DAVLVF010000000
VERSION     DAVLVF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.138"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..346)
                     /locus_tag="VYN66_001942"
     CDS             complement(<1..346)
                     /locus_tag="VYN66_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495906.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYE"
     gene            complement(404..1117)
                     /locus_tag="VYN66_001943"
     CDS             complement(404..1117)
                     /locus_tag="VYN66_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV1495907.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1575..1955)
                     /locus_tag="VYN66_001944"
                     /pseudo
     CDS             complement(1575..1955)
                     /locus_tag="VYN66_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
BASE COUNT          725 a          424 c          339 g          700 t
ORIGIN      
        1 gttcgtaggc atattttgct acatgatcta ggtatatatc aaaaaagtca tgaggaaaat
       61 ctcccataca gccattacga ttcatattga ggctatattt tcgattagta agataattcg
      121 gaaatatatt tcctattact ctaagattat ctacttgtga atcccttctc ccaatataat
      181 ccccttcttt gactaatgat ggagatttcc aagtaaaaat taaatcgcca gagaaatttt
      241 tcttatcaaa aaacttaaaa agagattgat aaatactatt tgctaattga caagtgtcag
      301 atgtaaattc ataattaaca gcttcattaa aatcaacttc agacatttca atgtactctt
      361 caatagtaat attagctttt ttgtagtcat tagctcgtct cctttaaaat gtgatctttt
      421 caagaatatt tttatctaaa caatccagca agtcttggta agaatagact tcgtaagtcg
      481 gctgggcttg tgtgtgattt tcgaggtgat gaggattata ccagatagtg tcaatccccg
      541 cattattgcc accttgaatg tcggcggtta gagaatctcc aatcatcagc gtcttttctt
      601 tactaaatcc agcaatttgc tggccaatct tttcataaaa aagagcatcc ggcttttgag
      661 tttgcaactg ttctgagata aagacttgat tgaaataagg tgctagacca gattgagcca
      721 aacgtcctgt ctgaatggca gtaatgccat ttgtcgcagc atacaagtca taatcacgct
      781 caatgaggct gtccaagaga tcatgagcgc ccgatagtgt ttgtccctgc tgagcgagat
      841 aaaattggta acgctgggca agaaaactac cgtctttttc ctgtccaaaa tgagaaaata
      901 aacgagaaaa gcgcgtgtta accagctctt gtttactgat tttcttcagc tccaagtctt
      961 tccagagagc cttgttcata ggaacgtaat aatctttata agcctgaata tccgcaactc
     1021 cttcttcttt tagaagttgg gtcaaagcca catcctcagc agcatcaaaa tcaagaagag
     1081 tgtggtcgag gtcgaagagt agaaatttgt agaacaattt gaggttttcc tttctgaaaa
     1141 ttcattaaga acattatatc ataaagcacc tcatacaatt aactaattta atcacttaaa
     1201 aaaattcgaa cactttctat acaactgaca gctcaaatct ttcagaatag aacaatacta
     1261 actatcgagc accccgtatt cataaatata tgtgtaattc tagacctaga attcctataa
     1321 actaaatgct ttcatactct tccaagtaat tgattgcctt aaattttaat ttttgaaggt
     1381 ttctaaagct agaatagccc catcacaatc agttttgatt gattcacaat ttagaaacac
     1441 tatagtttca ctcctgttaa aataaaaagg aactgcataa agcaatccct ttctgatttt
     1501 gaaatcattt acttaacatt ttatagttga gataatcaat agcttaccta taaaaagagt
     1561 tatagtaaaa ttccttattt attgatttca agctccgcta actgtgtttg aataactgac
     1621 agttctgcac cagcctgaag aagagcagct gcattatagg caccttctac aattggaacc
     1681 ctgttgatga tgatactttt atcactgaaa tcagtcacca tttttaagtt cattttagca
     1741 gaacctaggt caaaaaaggc aagtaaagta tctgctgtat tttcggaaac aaccctatct
     1801 acttaatcaa aactcgttcc aattcctccg ccctcggttc ctcctacata agtaatcgga
     1861 acatctttag ctactttact aatcagttca acaacacctt ctgcaatgtg tttggaatgt
     1921 gaaacgataa caagaccaat accaatactt tccatcaaac caccccagtt tctaaaatag
     1981 cagtaaagag taatcctgat gagaatgatc caggatcaat atgtccaaga aaccatatgc
     2041 tcctaagaca agagccaaca gactggtcat caataatagt attgttcttt ttttcatcat
     2101 tactccttaa ctagtgttta actgattaat tagccagtaa atagtttatc tttatttaca
     2161 ctatctgtta agatatagta aaataaga
//
LOCUS       DAVLVF010000139         2165 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVLVF010000139 DAVLVF010000000
VERSION     DAVLVF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.139"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..1151
                     /locus_tag="VYN66_001945"
                     /pseudo
     CDS             1..1151
                     /locus_tag="VYN66_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1266..2069)
                     /locus_tag="VYN66_001946"
     CDS             complement(1266..2069)
                     /locus_tag="VYN66_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV1495908.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
BASE COUNT          691 a          450 c          363 g          661 t
ORIGIN      
        1 aaaattcctt accttgaaac gactggtatg cctactagaa ttctccttag aaaacgtcgt
       61 tttaagtgct atcattgctt gaaaatgatg gtcgctgaaa cttctatcat caagaaaaat
      121 caccaaattc ctcgtattat caaccaaaag attgcgcaaa agttaattga aaagatttct
      181 atgaccgata ttgcccatca gctggccatt tcaacttcaa ctgtcattcg aaagctcaat
      241 gacttccact ttgagtgtaa ttttagaaat ctgcctaaga ttatgtcttg ggacgttgaa
      301 acagtccggg gagtgactgt ttcaatcggg agatggagat gagctttatt gcgcaagatt
      361 ttgaaaagct cgatatcatc actgttcttg agggtagaac acaagctgtc atccgagatc
      421 actttcttaa atatgataga gccgtccgat gtcgcgtcaa aattattact atggatatgt
      481 ttagtcctta ctatgactta gctaaacagc ttcgctttca aatttctagg ctcaggctga
      541 aacagtctcc cagactgttt cactcccgaa tgctaaaatc gttcttgatc gctttcatat
      601 tgtacaacat cttagccgtg ctatgagtcg tgtgcgtgtc caaatcatga atcagtttca
      661 tcgaaaatcc catgaataca aggctatcaa gcgctactgg aaactcattc aacaggatag
      721 ccgtaaactc agcgataaac atttttatcg ccctactttt cgtatgcatt taaccaataa
      781 agagatttta gacaagcttt tgagctattc acaagacttg aaacatcact atcagctcta
      841 tcaactcttg ctgtttcact ttcagaataa ggaaccggag aaatttttcg aacttatcga
      901 ggacaatctt aagcaggttc atcctatttt tcagactgtc tttaaaacct tcctcaaaga
      961 taaagaaaag gttatcaacg cccttcaact acactattct aatgccaaac tggaagcgac
     1021 caataatctc atcaaactta tcaagcgcaa tgcctttggt tttcgaaact ttgaaaactt
     1081 caaaaaaacg gatttttatc gctctgaaca tcaaaaaaga aaggacgaaa tttgtccttt
     1141 ctcgaactta gcttttcttc aacccactac aattgacaaa gagccaaact ttgatgatat
     1201 acgttttctt gtgagaatat ttacttcatt tttgcctaaa attcaatgtt tactcagtat
     1261 ttggattatg aaaaatcaag gtctaaatct agatacattt tttctgaaga caaatcattt
     1321 tgaccaccga gcaagagatt ttcaaaaaaa gctgttaaaa actcaggacg tcgctgtaaa
     1381 atctttgcat tatctaatac caaggcatca cgaaaatatt tggaatgttg ctgaaatggt
     1441 gtattatcaa tatcaaaacc aaactcacga agatactgaa tcaaaaagac cgttactgtc
     1501 cgagtgtttc cttcgcgaaa tggatgaatc tgccagattc ctgaaataaa atgctggatt
     1561 tgtttaacca catccgcctg agttagtgtc gcatatgcaa cttgtttttc ctgattaaaa
     1621 tcataatcta aggtcatttg aatcatggag taatcagagt acacaacact ttcaccattc
     1681 aaaacaggtt cattctttgt gatattggtc tgacgaaatt gccccaccgg aatagagggt
     1741 tcaaatatat cttgaaacaa ctccttatga atagcaagta aggtcgcagg actaaagcta
     1801 aagcctcttc gagacaatag ttctacaata cgtagagaaa ccaagtctgc ctcttccgta
     1861 cttgcatcaa tagtatggtg ataagccgtt gcatcctcat aaacctgctc ataagtcagt
     1921 tctccccggg actgtttctc agccaaagat tccatatacg ctgatggcac tagattgtca
     1981 actttctgca gaccaaaacc tatccgccat aaatcacgct tcgcttcata agacaagttt
     2041 ggattgtcaa tgttgtaagt tggttgcata gaaatatcct ttcaattgtt tttcttcatt
     2101 accagtaaat cacgaaattg gttggaaact ggcttaaact ataaaaagtc ctcacaatag
     2161 gttct
//
LOCUS       DAVLVF010000140         2054 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVLVF010000140 DAVLVF010000000
VERSION     DAVLVF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.140"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..1022
                     /locus_tag="VYN66_001947"
     CDS             <1..1022
                     /locus_tag="VYN66_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1495909.1"
                     /translation="NKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDHAF
                     GQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELN
                     GKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLW
                     RDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTS
                     KFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGD
                     DKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNA
                     CKNAK"
     gene            1012..2019
                     /locus_tag="VYN66_001948"
     CDS             1012..2019
                     /locus_tag="VYN66_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1495910.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
BASE COUNT          728 a          292 c          365 g          669 t
ORIGIN      
        1 aaaacaaatt ttttctaata gctattttag ctatgtgtat agtttttagc gcttgttctt
       61 ctaattctgt taaaaatgaa gaaaatactt ctaaagagca tgcgcctgat aaaatagttt
      121 tagatcatgc tttcggtcaa actatattag ataaaaaacc tgaaagagtt gcaactattg
      181 cttggggaaa tcatgatgta gcattagctt taggaatagt tcctgttgga ttttcaaaag
      241 caaattacgg tgtaagtgct gataaaggag ttttaccatg gacagaagaa aaaatcaaag
      301 aactaaatgg taaagctaac ctatttgacg atttggatgg acttaacttt gaagcaatat
      361 caaattctaa accagatgtt atcttagcag gttattctgg tataactaaa gaagattatg
      421 acactctatc aaaaattgct cctgtagcag catacaaatc taaaccttgg caaactttat
      481 ggagagatat gattaaaatt gattcaaaag ccttaggtat ggaaaaagaa ggtgatgagt
      541 taatcaaaaa tactgaagct cgtatatcca aagaattaga aaaacatcca gaaatcaaag
      601 gaaaaatcaa aggaaaaaaa gtattattta ctatgattaa tgctgcagat acatcaaaat
      661 tctggattta tactagcaaa gatccaagag caaattattt aacagattta ggtctagttt
      721 tccctgaatc attaaaagaa tttgagagtg aagatagttt tgcaaaggaa atttctgcag
      781 aagaagcaaa taagataaat gatgctgatg taatcataac ttatggtgat gataaaactc
      841 ttgaagcttt acaaaaagat cctcttttag gtaaaataaa tgcaattaaa aatggtgccg
      901 ttgctgtaat tccagataat acaccgttag cagcctcatg cactccaaca ccactttcaa
      961 taaactatac tattgaagaa tacctaaatc ttttaggaaa tgcatgcaaa aatgcgaaat
     1021 aaaaaacaaa taaacctagg cataattttt gtaatctgcc taggtcttct tattacaata
     1081 tttttgtcat taaagcttgg aacaaaagaa attaatatca gagatttttt agcagctttt
     1141 ggaatgggca atacaaatga tgattttatt aaatcaatta tatataaaag aatacctaga
     1201 actatttttg caattttagc aggttctagt cttgccataa gcggtgtatt gatgcaatca
     1261 gttactagaa acccaatagc tgatccaggt atactcggta taaacacagg agcaagtctt
     1321 agtgtagtaa ttggtctttc ttttttagga atttcatcaa gcataagcca tataagtttt
     1381 gcaatcattg gtggcttagt aagtgcaatt tttgtatacg cgattgctgt aagcggaaaa
     1441 gcaggcctta cccctataaa acttgcctta tcaggaactt gtgttagtat ggctttaagc
     1501 agttttgtaa gttttttaat tttaccgaat aataacgtct tagacaaatt tagattttgg
     1561 caaataggta gccttggagc agctacatta tcttctatat ctacactact accttttata
     1621 attttaggtc acttgatagc tatatttatt tcatcagatt taaacgcttt agctatgggt
     1681 gatgaaatgg ctgttggtct tggagttaat gttaatagga taagatcact tgcaataatt
     1741 gcaagtgtgc ttttatgttc aagtattact gcaattggtg gacctattgg cttcgtaggt
     1801 cttatagttc ctcacttttg tggcttattt ataagcaaag atatacgcac aatgaccatt
     1861 tcttcatctt ttataggtgc agagctcttg cttatatgtg atataatcgg ccgtatgtta
     1921 ggtaaaccag gtgaaattga agtagggata attactgcaa taatcggggg tccagtactt
     1981 atttatgtaa ctatgaaaaa tagaggggtt aataactaat gcaaaattta attataggta
     2041 ttcaaaaaag aaaa
//
LOCUS       DAVLVF010000141         1859 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVLVF010000141 DAVLVF010000000
VERSION     DAVLVF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.141"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(128..1758)
                     /locus_tag="VYN66_001949"
                     /pseudo
     CDS             complement(128..1758)
                     /locus_tag="VYN66_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
BASE COUNT          565 a          365 c          394 g          535 t
ORIGIN      
        1 tagaggtgta ctattctagt ttcaatatac tatatatctg cagaatactc tttcagtttc
       61 attcagccta gtcttgctta ggaaaaagta agaagccatc caattatgat ggatagcttc
      121 ttggttatta gatttgttct gcaaaaacct tttcagcaag gttcatagcg tggtctgata
      181 cacgagtgta gtgtgagatg atgtcgataa agttgacccc agcttgtgtt gaacattcgc
      241 ctttgttgag gcgtttgatg tgggtttttc tgagaacacg ttctatctta ttgattgctt
      301 catgacgttc tacaagactg cgtgcttttt caatatcatt gttttccaca ctatccagag
      361 catctttgat aaagtcacta gtttggcggt aaacttcctc taattctttc aaggcggcat
      421 cagaaaattc aacatttttc cgttgaagat agtcagtcag attgagtaga gcctccgtgt
      481 ggtctccaat ccgttccaaa tcacgggagg aatcaaggat attggtgagc acttcacttt
      541 ctttttggct gagagcttcg cttgaaaggg caatgagata acgtgttaat tgctcatcga
      601 tggtgttaat tgcttcttcg gttttatgcc ctttttcagc aactttttca tccaagtcaa
      661 tgatgtactt ataggaaagg tcaaaggctt tagcagcgta gtttcctaag tgcaagagct
      721 cttttttagc atttcctaga gcgatagatg gggcttgttt gatgaaatgt tcatcaagat
      781 ataagggttc gtatttgaca acctcgtcct ctccaggaat aatcttggtt acaaagtaag
      841 ccagagctcc gataaatgga aattggacaa tggtgttggt aatattaagg ttccgtgagc
      901 aaaggcgatg gtcatttccg gtgctagatt tagcgtagct tcaaaccaat gaatcaggac
      961 agtaaaagga actagaaaaa taacgcagac gactgttccg ataacgttga aggcaacatg
     1021 agctcctgct acccgtttag ctgcaatatt agcccctaaa gaggcaatga tggctgtaat
     1081 ggttgtcccg atattgtcac caaatagaac tggcaaagct ccctgtaggt caattagatt
     1141 gccggcgtag aggttttgta aaatcccaat ggtagccgaa gaagcttgaa ttagcaaggt
     1201 caagccggta ccgacaaaga cacccaaaac aggattctta cttagctcaa tcatatagtc
     1261 cttaaagacc tgtaaatcct tgagtggagc cattgcgccg ctcatgagat tgagggcaaa
     1321 aaagatacca ccgacaccaa agaggatgcg tccgatatta ttgactgtcc gatttttcgt
     1381 aaaaaagaga cagacggcac cgataaagag cataggtagg gcatagttac ctaatttaaa
     1441 accgatgaga aaggatgtga cagttgtccc aatattagca cccatgacaa tcccgatagc
     1501 ctgacgtaag gttaagagac cggcactgac caggccgact gtaataactg ttacaccaga
     1561 actagactga attagagcag tcatcccaat accaaccaga actccaaaga aaggattact
     1621 agtatatttg tcaatgtaaa aacgaaggcg atctccagca gcttgttgca aaccgtctcc
     1681 catggtcttg atgctatata agaatagccc cagaccacct aaaaagtgaa ataaaatttc
     1741 ctgccaatta atggacattt ctttttcctc cgaaaaataa tcgcggaata tctcctattc
     1801 tattttaaag gataaaagta aatctaacaa gtgttaatgt caagtttgta aaagaaaat
//
LOCUS       DAVLVF010000142         1793 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVLVF010000142 DAVLVF010000000
VERSION     DAVLVF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.142"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            365..841
                     /locus_tag="VYN66_001950"
     CDS             365..841
                     /locus_tag="VYN66_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1495911.1"
                     /translation="MIGKNIKSLRKTHDLTQHEFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            841..1602
                     /locus_tag="VYN66_001951"
     CDS             841..1602
                     /locus_tag="VYN66_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV1495912.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLQELFFGEWSQVEKEMLQVGEKRLNELLEK"
BASE COUNT          661 a          241 c          346 g          545 t
ORIGIN      
        1 agtaaaaaca acactttatt gaggtattgg atacgctttg ctaatagcct aataatcaca
       61 tgtggagtgt tgctacaacg aaaaaggcga taatccttga tttcaaacta ttttataagc
      121 attttgtctt tgtagataaa ggcaattttg acaataaaaa tcctaaaagg tgaatcgtta
      181 tagatgtatt tgtagatatc gtttgcgcat cgaaaaaatt aatacaagaa taaatattta
      241 tggctcttta ggtgactttt atagaagtaa agtttaggat agaaaaacaa gaaataatgc
      301 accatttttg gtgcattatg cttttttatg ctataattga attataaaaa taaaggagtt
      361 tgccatgatt ggaaagaaca taaaatcctt acgtaaaaca catgacttaa cacaacacga
      421 atttgcacgg attgtaggta tttcacgaaa tagtctgagt cgttatgaaa atggaacgag
      481 ttcagtctct accgaattaa tagacatcat ttgtcagaag tttaatgtat cttatgtcga
      541 tatcgtagga gaagataaaa tgctcaatcc tgttgaagat tatgaattga ctttaaaaat
      601 tgaaattgtg aaagaaagag gtgctaatct attatctcga ctctatcgct atcaagatag
      661 tcagggaatt agcattgatg atgaatctaa tccttggatt ttaatgagtg atgatctttc
      721 tgatttgatt catacgaata tctatttagt agaaactttt gatgaaatag agagatatag
      781 tggctatttg gatggaattg aacgtatgtt agagatatct gaaaagcgga tggtagctta
      841 atggaaatcc aagattatac tgatagtgaa ttcaaacatg ctttagcaag gaatcttcgt
      901 tcactgacaa gaggaaaaaa gtccagtaag caacctatag cgattttgct tggaggtcaa
      961 agtggtgccg gtaagactac aattcatcgt attaaacaga aagaatttca aggaaatatt
     1021 gttatcatag atggtgatag ttttcgttct cagcatccac actatttaga actgcagcaa
     1081 gaatatggca aagacagtgt agaatatacc aaagattttg caggaaaaat ggtagagtct
     1141 ttagtaacaa aattgagtag tttgggatac aatcttttga tcgagggaac tttacgaaca
     1201 attgatgttc caaagaaaac ggcacaactc ttgaaaaata agggatatga agtacaattg
     1261 gccttaattg cgacaaagcc tgaattgtcg tatctaagta ctcttatccg ttatgaagaa
     1321 ctgtacatta tcaatccaaa tcaagcacgc gcaactccaa aagaacatca tgatttcatt
     1381 gtaaatcatc tagttgataa cacacgaaaa ttggaagaac tagctatctt tgaaagaatt
     1441 caaatttacc aacgagatag aagttgtgta tatgattcaa aagaaaatac aacttcagca
     1501 gcagatgttc ttcaagagtt attctttggg gagtggagtc aggtagagaa ggagatgttg
     1561 caggtggggg aaaagagact taatgaatta cttgaaaaat aaacaattga tatttttagg
     1621 agaatagaaa tgagagggtt taataacaag ataaagtctg tttatcaaga actaacaaat
     1681 tccaaagaga aattcggtag ctttcacaag actttaattc atttgcatac acctgtttct
     1741 tatgattaca agctattttc taattggact gcaacgaaat atagaaaaat tac
//
LOCUS       DAVLVF010000143         1785 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVLVF010000143 DAVLVF010000000
VERSION     DAVLVF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.143"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(135..803)
                     /locus_tag="VYN66_001952"
     CDS             complement(135..803)
                     /locus_tag="VYN66_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV1495913.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFANKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLIIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(914..1705)
                     /locus_tag="VYN66_001953"
     CDS             complement(914..1705)
                     /locus_tag="VYN66_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEV1495914.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLVFTSF
                     AKEIQEELFTQEQFHFIAEVVLADGGLDKEIDKVGLSTLERSFRALIYANLLSADANQ
                     QSVFYQGLQSEIRNVLLNQGLHYLSKEKDTTGFSSQYGWVHSFAHGADLLTEVVCHPD
                     FPINRIHEVFDILGQLFKRMSILFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFYKFSNFRSCLVEVYVQLDQRNSLQDDLKEAIQSFQY"
BASE COUNT          591 a          386 c          319 g          489 t
ORIGIN      
        1 tagatgaaac tgacgaagtc agtaaccata cctacggcaa ggtgaagctg acgtggtttg
       61 aagagatttt cgaagagtat catacgaaaa agccctgttc ctcaagatga ggagcaaggc
      121 tttttgagtt tcaattattc ttctgttcca aggaaatttt ttgcaacaag ggctgcaaga
      181 actccaccag cgattggtgc aaggatgaaa atccaaactt gttgaagggc tgcgccgcct
      241 accaagacag ctggtgccaa gctacgagct gggtttactg aaagtccagt aatcttcagt
      301 ccgacaagaa tcatcgccat caatgacaaa ccgattacca aaccagcaat cgcgccattg
      361 cccttacttt ctgaagtcac agtcatgata atcaagacaa acaagaaagt tgcgatgact
      421 tcaaacaaga aaccaccaaa gacagtgaca ccgtttgcca aggcattttc accaagacta
      481 gcagttgaca tacctgaatt acccaagagg aagaagactg cgccagaagc gatgaaagct
      541 ccaacaacct gaccaaggat gtagtttaca agttctgaag atgacaaacg tttgtttgca
      601 aacatagcaa tcgaaacagc cgggttcaag tgagcacctg aaacagttcc gattgagtag
      661 gctgccacca cgattgccaa accaaaggca aaggcgattc caaggtgacc aaggccatca
      721 agaccatttc caaaaacaac agctcctgtc ccaacgaaca caagcatgaa cgtaccgatt
      781 aactcagcaa caaatttttt cattttcttt ctcctttttt caaaaactag atactagtct
      841 atcaaaagta ggaaagggtt tcaagaaaat tgattggaaa ttttttgaaa atcatagaac
      901 tattagctaa tccctagtat tgaaaagact ggatagcttc tttcaggtca tcttgtaaac
      961 tatttctctg gtcaagttgg acatagactt ccaccagaca ggatctaaag ttggaaaatt
     1021 tgtaaaaatc ctccctttct tctatcggaa aatcaacagt ttttatccaa gaagctactt
     1081 gttcttgctc caacttccct tgtaaaatag gttcatagat cactcttgct aaacgccaat
     1141 cctcatcatc tgtaaagaga atcgacattc ttttaaatag ttggccaagt atatcaaata
     1201 cttcatgaat tctgtttata ggaaagtctg gatgacagac cacctctgtc agtaaatcgg
     1261 ctccatgtgc aaaagaatga acccaaccat actgacttga gaaacctgtt gtatcctttt
     1321 cttttgaaag atagtgcaag ccttgattta aaaggacatt acgaatttct gattgtaatc
     1381 cctgataaaa aaccgattgc tggttagcat ctgcagacaa gagatttgca taaataagtg
     1441 ccctaaaaga acgttcaagt gttgacaagc ctaccttatc aatctcttta tctaaccctc
     1501 catcagctaa gaccacctca gcaatgaaat gaaattgttc ctgtgtaaat agctcttcct
     1561 gaatctcctt agcaaagctt gtaaaaacaa ggtcatcacg aatttctgga gaaggatttc
     1621 ccaaatgatc aaacaaccac tggatttctt cctggttata atttggtttt tcttctgcta
     1681 tttttcttag taaatcttga tacatggtca atacctctac atttctagca actgttcaaa
     1741 aaggcagtct taaatgactt taccaatttg taaaattaag ttaaa
//
LOCUS       DAVLVF010000144         1750 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVLVF010000144 DAVLVF010000000
VERSION     DAVLVF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.144"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(38..274)
                     /locus_tag="VYN66_001954"
     CDS             complement(38..274)
                     /locus_tag="VYN66_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEV1495915.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCHRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            540..1337
                     /locus_tag="VYN66_001955"
                     /pseudo
     CDS             540..1337
                     /locus_tag="VYN66_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1372..>1750)
                     /locus_tag="VYN66_001956"
     CDS             complement(1372..>1750)
                     /locus_tag="VYN66_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1495916.1"
                     /translation="RGKVSGRRYQRISLVAGLTNGELIAPMTYEEMVTSDFFEAWFQK
                     FLLPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAYIKK
                     NLKKVLPSCNTFYEALFSCSCFN"
BASE COUNT          621 a          349 c          249 g          531 t
ORIGIN      
        1 aaagagtaaa ttagttcgga aatttactaa aattacctca acaagaaata aaaccccgat
       61 tcattaccaa tttttcaaga tacaaacgat aagcaacacg ataatggtaa acgataaaat
      121 ccctatgaca accaatgcca tatctcacta aataataaat taaaaattta aaatgaacat
      181 gttcccagtc aaaattatca ccaaatgtag gaccatactc ttcttcaata ctatcataga
      241 aattagccat ctgctcataa attttttgta acataatcaa atactccttt tcttttttat
      301 aaacttattc taacaaaaaa ttttacacat tcactatcaa ttcctgaatt gttaaaatac
      361 cagcctcaac aagataaaaa taggaaaagt tgacaaaata gaaacaaatt tgctacctaa
      421 atttcaaaca attaaagttc taaaaagacc gccttaaaac ttttgggtaa atcctattag
      481 aactataata tttttatata ggtattatgg ttctgatttt ctagtataaa aaaattcaaa
      541 ctagaattct tcttagaaaa ggccgtttta agtgctatca ttgctcaaaa atggagctcg
      601 ctgagacttc tatcgtcaag aagaatcatc aaattccttg tatcatcaac caaaagattg
      661 ctcaaaagtt aattgaaaag atttctatga ccgacattga tcatcagttg tctatttcaa
      721 cttcaactgt cattcgcaag atcaataatt ttcactttga gcatgatttt tcgcgtcttc
      781 ctgagattat gtcctaggac gttgaaacag tccggggagt gactgtttca atcgggagat
      841 ggagatgagc tttattgctc aggactttga taaactcaat atcataactg ttcttgagag
      901 cagaacacaa gccatcatcc gaaatcccat gaatacaagg ctatcaagcg ctactggaag
      961 ctcattcaac aagatagtca gaaactgagt gataaacgat tttatcgccc tacttttcgt
     1021 atgcacttga ctaataaaga aattctagat aaacttttga gctattccga agacttgaaa
     1081 caccactata atctctatca gctcttgctt ttccacctct gtttcagact ttgtttaaaa
     1141 ccttcctcaa agataaagag aaaatcgtca acgcccttca attaccttat tccaacgcca
     1201 aactggaagc caccaataat ctcatcaaac ttatcaagca caatgccttt ggttttagga
     1261 actttgaaaa cttcaaaaaa gaaaggacga aatttgtcct ttctagatct tcgctttctt
     1321 caacccacta cagttgacaa agaaccgaaa aatgtctcgc ctctaatata gtcaattgaa
     1381 acaagaacaa gagaaaagag cctcgtaaaa ggtattgcaa cttggtaata cctttttgag
     1441 gttctttttg atataagccc atgttttctc aataggattg tactcaggtg agtagggagg
     1501 aagaggtaaa agtttatgcc caaactcctc gcataaaagt tctaacttac ccattctatg
     1561 gaatcttgca ttatccataa taataaccga tggtgtggtt aatgttggta agagaaactt
     1621 ctgaaaccaa gcttcaaaaa agtcgctcgt caccatctct tcgtaagtca ttggagcgat
     1681 taactcacca tttgttagac ctgcaaccaa agaaatcctc tgatatcttc ttccagatac
     1741 tttacctctt
//
LOCUS       DAVLVF010000145         1748 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVLVF010000145 DAVLVF010000000
VERSION     DAVLVF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.145"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..68
                     /locus_tag="VYN66_001957"
     CDS             <1..68
                     /locus_tag="VYN66_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEV1495917.1"
                     /translation="RFSTFSTNRCGNWSAFSWENC"
     gene            364..1715
                     /locus_tag="VYN66_001958"
     CDS             join(364..805,805..1715)
                     /locus_tag="VYN66_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1495918.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSISLDYALPNQGMLSFWPAQYKKN
                     GYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENERLRTEVAYPKKVKRVRGKGRS
                     PRARKAETVREMVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDN
                     EHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQGEIGKKAE
                     NLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPNLEQ
                     VKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNGMME
                     SFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG
                     "
BASE COUNT          594 a          273 c          390 g          491 t
ORIGIN      
        1 tgcgatttag tacattcagt acaaaccgtt gcggtaactg gagtgctttt agttgggaaa
       61 attgttaatt catttaaatt gtaaacttta gggattctct gaagttatgg atgtcgttcc
      121 aatatgtgta cgttggaatg ttagtgctta tatgggggga acagaatcct ctcttgattg
      181 aagacaagtt agccattagg ctggtttgtc tctttgtcaa ctgtagtggg ttgatataat
      241 agtattagtg agtgggataa aagtttcatt tagtttattc agtacaaatt taacgggtca
      301 agatttattg aactgcaccc caatagttag acagaaaaaa tctaactttt ggggtgtttt
      361 attatgaaat tgagttatga agataaagtt cagatctatg aactaagaaa gcaaggacaa
      421 agcttcaaac agctttcaaa aagatttggt gtggatgttt ctggtctaaa gtacatggtg
      481 aaattaattg accgttatgg catagaaatc gttaaaaaag ggaagaatcg tcactattct
      541 tctaaattga aacaagaaat gatggataaa gccttactgg aaggttgttc acaaagaagt
      601 attagtcttg attatgccct cccaaaccaa ggaatgcttt cattttggcc ggcacaatac
      661 aagaaaaacg ggtatactat tgttgagaaa acaagaggga gaccagctaa aatgggacgt
      721 aaacggaaga agacttggga agaaatgaca gaactagaac gactccagga ggagaatgaa
      781 cgcttacgga ctgaggtggc ctacctaaaa aagttaaaag agttagagga aagggacgaa
      841 gccctagagc gagaaaggca gagacagtta gagaaatggt ttcaggagga tttcgactag
      901 attttcttct tgaaacagca cgtttagctc gctcgactta ctattatcag ttgaaacaac
      961 tggatggggt tgataaagat aaagagatta aaaccgaaat tcagggcatt gataatgaac
     1021 ataaaggcaa ctatggctat cgtcgaatcc atttagaact aaggaatcgt ggttttgtgg
     1081 tcaatcataa gaaagttcaa cggttgatga gaattcttgg tttaacggct cgaattcgcc
     1141 gtaaacggaa gtattcttcc taccaaggag agattggcaa gaaagcagag aatctcattc
     1201 aacgccagtt cgaagcatcg aggccaatgg aaaagtgcta tacagatgtg acagagtttg
     1261 ccattccaaa tagcacacag aaactctatt tatcaccagt tttagatggt tttaacagtg
     1321 aaatcattgc ttatcatctt tctacttcgc ccaacttaga acaagtgaag agtatgttag
     1381 aacaagcctt tacagagaaa tactatgaga atacgattct ccatagtgac caaggctggc
     1441 aataccaaca cgattcttat catcggttcc tagagagtaa gggaattcaa gcatccatgt
     1501 cacgcaaggg caacagccca gacaacggca tgatggaatc tttctttggc attttgaaat
     1561 cggagatgtt ttatggttat gagaagaact ttagatcttt agaaaacctt gaacaagcta
     1621 ttgtggacta cattgattac tacaacaaca aacgaattaa ggtaaaacta aaaggactta
     1681 gttctgtgca gtacagaact aaatcctttg gataaattat ttgtctaatt ttttggggtc
     1741 agtacagt
//
LOCUS       DAVLVF010000146         1743 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVLVF010000146 DAVLVF010000000
VERSION     DAVLVF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.146"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..619)
                     /locus_tag="VYN66_001959"
     CDS             complement(<1..619)
                     /locus_tag="VYN66_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1495919.1"
                     /translation="MEQLHFITKLLDIKDTNIQIIDVVNRDSHKEIIAKLDYDAPSCP
                     ECGNQLKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFKHDFSCLPEIMSWDEYA
                     FTKGKMSFIAQDFNNLNIITVLEGRTQAIIRNHFLHYDRAVRCRVK"
     gene            982..1581
                     /locus_tag="VYN66_001960"
     CDS             982..1581
                     /locus_tag="VYN66_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495920.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNLSLYTTIDKTSDATALLKLKLNNVDNQPATEVLSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNYP
                     VGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
BASE COUNT          584 a          265 c          341 g          553 t
ORIGIN      
        1 ttttgacgcg acatcggaca gctctatcgt agtgaagaaa gtgatttcga atgatagctt
       61 gtgttctacc ctcaagaaca gtgatgatat tgagattgtt aaaatcttgc gcaatgaagc
      121 tcatctttcc ctttgtaaaa gcatactcat cccaagacat aatctcagga agacaagaaa
      181 aatcatgttt aaagtgaaaa tcattgagct tacgaataac agttgaagtt gaaatggcca
      241 gctgatgagc aatatcggtc atagaaatct tctcaatcaa cttttgcgca attttttggt
      301 tgatgatacg agggatttgg tgattcttct tgacgataga agtttcagcg accatcattt
      361 ttgaacagtg atagcacttg aaacggcgtt ttctaaggag aattctagta ggcataccag
      421 tcgtttcaag ataaggaatt ttagaaggtt tttgaaagtc atatttcttc aattggtttc
      481 cgcactcagg gcaagatggt gcgtcgtagt ccagtttggc gatgatttct ttgtgtgaat
      541 ccctattgac gacatctata atttggatat ttgtgtcttt gatatcgagt agttttgtga
      601 taaaatgtaa ttgttccata tgaatctttc taatgagttg tttgatcgct tttcattata
      661 gatcttatgg gacttttttt ctacacaaaa ataggctcca taatatccat aggggattta
      721 cccactacaa atattataga gccgttaata tttggagaag aggatggcca tcttctccat
      781 ttttatgtgc agagttatag tgacttgatg ctgggaaagt acactgtgac tgctaatact
      841 caatgaaaat caaagtgcaa actaggaagc tagccgcagg ctgtacttga gtacgtcaag
      901 gtgaagctga cgtggtttga atttgatttt cgaagagtat aaaacattcc tagaagttgc
      961 tttgatttcc ctaatctatt tatgcaaatt ttatgttatt ttactataac agttgttgct
     1021 aagccaaata atagtgggga agttcattta gacgtaagca ttgaagataa tcagggaggt
     1081 agtgggcata atttcagttc tgtttcaagt agctcacaaa cagctaaata tgaagaaact
     1141 ggttataata acaatttatc attatataca acgattgata aaacgtctga tgcaacagct
     1201 cttttgaaat taaagttgaa taatgttgat aatcaacctg ctactgaagt tcttagttca
     1261 ggaattactg taaaattaaa tgctaaagat aatgctggaa actggacaag tgcttcgaat
     1321 aaaaaagaaa taacagtaaa aattgtttct gctaaaccga catatccaga caaaatctta
     1381 gtgaaaaatc ctgataatat aaaagataca gaaaaaaatg ccattattga aaaattgaaa
     1441 gaggcaaata aaaattatcc agtaggagct ccaacctttg ctaaaggtga aggagagcat
     1501 gcaaatgata ttgtagcaac ttattcagat ggtacaactt attatgtacc gttaaatgat
     1561 gtgacaaaat atgcgaggta gtggctgtac cactcactta ttcacctccc cgtgatttgt
     1621 agtagtgata ggctttctaa ctattattat aaaacaaaat aaagagcaca acactttttc
     1681 attctgtgtt gtgccttgag tgaaacgaaa ggaatgaatt ataaatatga aaagtatagt
     1741 cac
//
LOCUS       DAVLVF010000147         1707 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVLVF010000147 DAVLVF010000000
VERSION     DAVLVF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.147"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(644..1636)
                     /locus_tag="VYN66_001961"
     CDS             complement(644..1636)
                     /locus_tag="VYN66_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1495921.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
BASE COUNT          594 a          276 c          213 g          624 t
ORIGIN      
        1 gtagttataa aaactcattc agataattta ttatttgatt ttgatacgat tcctgaaaat
       61 ataatagtag ttgaccattt tgaacttaca atcgaaaaat ggttaagtaa aagtcaacag
      121 ttactatagt ttgcaaatta tttacagaaa gaacaaaaaa tgctctgaaa cactgatttc
      181 aaggcatttt ttgttagttt aaaattactc ccattcttct tttccaaacg tacaatatat
      241 ccaaaaccat tcaaaatact agattctatt ttttataata tcactaaatc cacctaatta
      301 taggacgttt tcagattttt agtcccgtcc cagtcccagt accggagaaa tattgtttta
      361 atataatatc tctttttgtc ttctaagctc ttaaaagcaa aagaacaagt aaagagtcaa
      421 gacaaggata aaaagtccat attagggcaa ataaaaagct ttaagacaga tgacaaatct
      481 aagtcaaata agaaagacca tagcaaaggt gcagagagat aaatattggc ggtcttcgga
      541 ctgcctttat ttttttatcc atttttcaaa tcaaatttat tcagactata tatgcacata
      601 tacacttaaa ttcatataaa aacatggctt gtaaaaaatt actttaatca caataatcgc
      661 atttaaaatt gtgatgtttg caagctaaat tacggacttc acttggaagt tttcccttgt
      721 atcttttata atagatagaa aatttgctgg cagatgaata tccaacagat tctgctatct
      781 cttttatagg tagttcagtg tttaaaagaa gagtttcagc tacattcatt ctttttcttt
      841 gagtgtactc tgtaatgctt tgacaatatt tttccttaaa taaatttttt aatttagtac
      901 cactcatttt agatattttt tcaagcgtgc cttgatttac attcgttgca aaatgatcat
      961 ctaagaatct tgctacatct tcaagtgctt tatcatcatc aatttcaatt ttatattttt
     1021 ttctatttaa gtatgtgtca attactatac ttatccattc atttgccttt gctttaaaga
     1081 aaaaatcagc ggcaggagcg tccatcttac aatttaatat ttccattgcc actctttcta
     1141 aggcctttgt aagtattatt tgattcggtt gaagcaaggt tgaataaaaa gattctggat
     1201 taatgttaat agatgctaaa tgtttttcta ttagctcttt tttaaaacca atggaaacag
     1261 caagataaca acaattctcg tgtaataaaa aaacaaaatc atcttttata ttatcaaaat
     1321 caaaagtaca taaagagttt gcggtaatag tttgatacgg attaaacttt tctccgtttg
     1381 cactgacaat gtaacttgaa taaattgaaa catagtctga catactataa gtgctatttt
     1441 gaactacttc ctctttgata taaaaatcat gtatatcgat aatgaagctg cctccttcat
     1501 aaaaccaata taggccttct gcataagttg aattctcttt tttccaacaa aatgtatgac
     1561 ctgcacttga atacttctca ttgtttttac attcatctac tttcatataa tcttttacaa
     1621 aatcataata tgacataaca cactatccct tttagacaat attccaatta gccttattaa
     1681 ttcaaaacta ttgtattagt aattata
//
LOCUS       DAVLVF010000148         1680 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVLVF010000148 DAVLVF010000000
VERSION     DAVLVF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.148"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(778..1647)
                     /locus_tag="VYN66_001962"
     CDS             complement(778..1647)
                     /locus_tag="VYN66_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1495922.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIVA
                     AIAAVAVFKFLYL"
BASE COUNT          560 a          342 c          314 g          464 t
ORIGIN      
        1 taaaaattta actaattaat agaaagtact aaatagactt atccttcaag gagttttcag
       61 cattatccat ccactcactt gctgaaaatg taaacagtct ctggtttata tagtaaaatg
      121 aaacaagaat agagcaaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa
      181 gtagatgtgt actattctag tttcaatcta ctatacaacc tcaaaactgt gttttgaaca
      241 acctgcggct agcttcttag tttgcccttt gattttcatt gagtataaaa ttgcagataa
      301 aacgataaca atcataggac tcagcagagt caatgtaggt cgaagaatac ccccctaatc
      361 gcttatttta tagcaaaatg aaatagaaac tgaacatttt gttgaggaag gtaaatcaat
      421 tcctcacaat gtttgagaaa gaagggagga ctattccaac ttcaacccac tataaagcag
      481 acttacaaga tagggaaccc atctccgata atatatagta gaatgaaaac tgcacaattt
      541 gattggagaa ttcaaaccaa tttctcacaa tgttttagaa acaagggtct attatttcaa
      601 ctgcaaccta ctatagtcca ttgaagctgg aatagcccac tgagacttct aaaccactgc
      661 tggagaggag tttgtttaga gtttctttca tttccctaaa aaatcaaaaa gagagaacca
      721 gtctgatcct ctcttattta tagatggtgc tcactcaaat gttaaaggag ctatttctta
      781 gagataaagg aatttgaata cagctactgc cgcaattgct gctacgatag gtgctactac
      841 tggtacccaa gaataccacc attttgaatc gcctttatgc tcaccaagaa ctgattttgg
      901 aaggaaagca tgaaggagac gtggtcccaa gtcacgggct gggttcaagg caggtcctgt
      961 aggtcctcca agtgatgtta ccaaagccat aacgaggaat ccaagtgcca agtgagccac
     1021 agaaagtcct gaagcagtgt gtggagccac ctgtgcttta atagccaagt cagaaaaatc
     1081 aactgtttgt cctgcttcta ttgccttttg tttcatgaat tgaagcactt cagcaccaaa
     1141 gaagttttta gtcaaaccaa gagctgcaaa gaacaaaaca aatgaaccaa caaactcatt
     1201 aatcaaacca ttgacagttg ctgcatagcg actttctttt gtaccatggt caatacttga
     1261 aatagttgag aaagttccca agatgttatt tgggttttca gttttcaagt agaatggacg
     1321 gtatgttgcc acaactaagg cttggccaaa gatagctccc aagacttgcg cgataatata
     1381 aggtaccact tgtgcccaag ggaaaagacc gctaactgca agccctagag tgaaagcagg
     1441 gttgatgtga ttcccagata cgttaccaaa catcaaggct gggatcataa cccccatacc
     1501 ataaccaaca gcgatgacga tccagccact ttggtgacct ttcgtacctt taagttcaac
     1561 gttggcaact gcaccattcc caagaatgat caaaatggca gttcccaaaa attcagtggc
     1621 atacttcagt gcccatgtga aatccatttg ctagattctc cttaaaattt tttagatatt
//
LOCUS       DAVLVF010000149         1661 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVLVF010000149 DAVLVF010000000
VERSION     DAVLVF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.149"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            145..1491
                     /locus_tag="VYN66_001963"
     CDS             145..1491
                     /locus_tag="VYN66_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEV1495923.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
BASE COUNT          491 a          359 c          325 g          486 t
ORIGIN      
        1 ctaaaaacca tccagaatcc ttgccttagc ttagatcctg gatggtttct tttttcaccc
       61 aatgggtgtt ttttactaga caaaaaagag tttccccttt atggtataag tgtagaaaaa
      121 aacacaaaaa gaaaggaaac tcacatgaac agtttaccaa atcatcactt ccaaaacaag
      181 tctttttacc aactatcttt cgatggaggt catttaaccc agtatggtgg tcttatcttt
      241 tttcaggaac ttttttccca gctgaaacta aaagagcgga tttctaagta tttagtaacg
      301 aatgaccaac gccgctactg tcgttattcg gattcagata tccttgtcca gttcctcttt
      361 caactgttaa caggttatgg aacggactat gcttgtaaag aattgtcagc tgatgcctac
      421 tttccaaaat tattggaagg agggcagctt gcttcacagc caaccttatc ccgttttctt
      481 tccagaactg acgaggaaac agtccatagt ttgcgatgcc tcaaccttga attggtcgaa
      541 ttctttttac agtttcacca gctaaaccaa ctcattgtag atatcgattc tacccatttc
      601 acaacttatg gcaagcaaga aggtgttgct tataacgccc actatcgtgc tcatggctat
      661 catcctcttt atgctttcga ggggaagaca ggttattgtt tcaatgccca gcttcgtcct
      721 ggtaatcgtt attgttctga agaggcagac agctttatca cacctgtttt agaacggttt
      781 aatcaacttc tctttcgaat ggatagtggc tttgcgaccc caaaattata cgatttaatt
      841 gaaaaaacag ggcaatacta cctcataaaa ctcaagaaaa atactgttct gagccgtctt
      901 ggagaccttt ccctcccttg cccacaggat gaggacttaa ccatcttgcc ccactccgcc
      961 tactcagaaa ctctctatca agcaggatct tggtcgcaca agcgtcgtgt ctgccagttc
     1021 tctgaacgaa aagaaggaaa cttgttctac gatgttattt ctctcgttac aaatatgacg
     1081 agtggaacaa gccaagacca gtttcagctt tatcgtggac gtggtcaagc cgagaatttc
     1141 atcaaggaga tgaaggaggg attttttggc gataaaacgg atagttcaac cttaatcaaa
     1201 aacgaagttc gtatgatgat gagctgtatc gcctacaatc tctatctttt tctcaaacat
     1261 ctagctggag gtgacttcca aactttaaca atcaaacgct tccgccatct ttttcttcac
     1321 gtggtaggaa aatgtgttcg aacaggacgc aagcagctcc tcaaattgtc tagtctctat
     1381 gcctattccg aattgttttc agcactttat tctaggatta gaaaagtcaa cctgaatctt
     1441 cctgttcctt atgaaccacc tagaagaaaa gcgtcgttaa tgatgcatta aagaacagtc
     1501 gagatgaaaa aatcgtgtga cgaaccaagg gaggagtctg cccttttgag gaaatctagc
     1561 gaggaaaaac gatactggaa cagcagaaag taaaactgac ctcatgagga ggaagaaagt
     1621 ggctcatgag gtcaggggtt ttgtaagtta catctagttg a
//
LOCUS       DAVLVF010000150         1632 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVLVF010000150 DAVLVF010000000
VERSION     DAVLVF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.150"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(158..1597)
                     /locus_tag="VYN66_001964"
                     /pseudo
     CDS             complement(158..1597)
                     /locus_tag="VYN66_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
BASE COUNT          413 a          345 c          272 g          602 t
ORIGIN      
        1 gtgcttctac tttctctggc tcgactatcg ctccggcttg cacccctgtg tattctggta
       61 gtggtgctac ctgctctggt tcaacaatag cgccagcttg tactccagca tattccggtt
      121 caactttttc aggttcaaca atagctccag cttgtactcc tgtatattct ggtagcggtt
      181 ctacctgctc tggatcaccc ttgtcagtca tgactgcctc tggtaacgct ggttgaactt
      241 ctggtacgcc tttatcactt actacagctt ctggcaactc tggttggact gctgactcac
      301 ctttgtcggt taatacagct gctggtagtt caggctgaat tgcgggttca acaatagctc
      361 cagactgtac ttccttatgt tctgcaccag tctcagcttg ttcctttata acttgagctt
      421 ttttagtacc tttttcgact attcttgaaa caggcgcagt cgttgaagtt gaaataattt
      481 ctcgtgaaac ttcttcattg tttacagaaa atattctgac gatttcaact ttcttaccta
      541 atgcaccttc ttgttttact cttacagttc cttctgctaa atcaggattt tcttgaattt
      601 cttcttgaaa atctattttt gtttctatag tttcgtcacg atataagagt tccggtttgt
      661 tcaactgacc tgatagcact tcatcctgtg gatttaatgt atttacccca gtcttttcct
      721 ttggagaaat cttctcctct ttcttcgttt ctagattctt atgttcggct aattgttctt
      781 gagaatctga agattgtttc tcttcttttc ttggattgat taattcagta gagaaaggtt
      841 tttcaactac ttgaacttct gtcggcttag ttgaagaaac aggtgtttgt tcctgaatag
      901 cttgtactgt tgatggatgg tctacaaaat ttggtgtaac attataatcc accttttgtt
      961 gttttgtagg agtggctgct gatttttctt gattacttac ttcaaaatca gaagtcgttt
     1021 ttccctcttt gatatatcca atataagtgt aacctgaaat ctctttagga agaggtaatt
     1081 tttctccaga ggtcaattca tagtccgtat tgtaatttag caaaagatga ttttctaaag
     1141 catggactga aactaagaca ccatttccca ttcccgcaac caatactaaa tgtaataccg
     1201 ttttattctt aacctttttc ttggaaacag caaaaattaa aattcccaga gcagctaagc
     1261 tagcaccagc aactagggct tgcctctcat tcttgcttcc agtatttggt aattccgcca
     1321 gttgattttg agaatttaac ttataaacaa gataataagt ttcatcatca ttctccacgt
     1381 atgtcggaat atcatagaca agctgcttct tttcttctga tgatagctct gaatctgcca
     1441 catatttata gtgaactccc gcagtttctt gagcatccac agatgaacta gctaatacag
     1501 acataaaaaa taaacttgaa atcgttgcag atacaagtcc tactgataat tttctaaatg
     1561 aaaaacgctc ttgtttttca ccaaaatact tttccattat tcctccttga aataaaattt
     1621 atatatgtta ca
//
LOCUS       DAVLVF010000151         1613 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVLVF010000151 DAVLVF010000000
VERSION     DAVLVF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.151"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            44..>1613
                     /locus_tag="VYN66_001965"
     CDS             44..>1613
                     /locus_tag="VYN66_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEV1495924.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPQVVEKSSLE
                     KKYEEAKAKADTAKKDYETAKKKAEDAQKKYDEDQKKTEEKAKKAEEAAKEVDDASLA
                     VQKAYVEQRKAQEEKNKHSGNPEYAKKLAEADKKITEETTKLTDAQTKFQSVQAMVVP
                     EPEQLAETKKKAEEAKAEEKVAKRKYDYATLKVVLAKKEVEAKELEIEKLQYEISTLE
                     QEVATAQHQVDNLKKLLAGADPDDGTEVIEAKLKKGEAELNAKQAELAKKQTELEKLL
                     DSLDPEGKTQDELDKEAEEAELDKKADELQNKVADLEKEISNLEILLGGADPEDDTAA
                     LQNKLATKKAELAKKQTELEKLLDSLDPEGKTQDELDKEAAEAELDKKVEALQNKVAD
                     LEKEISNLEILLGGADSEDDTAALQNKLATKKAELEKTQKELDAALNELGPDGDEEET
                     PAPAPQPEQPAPAPAPKPEQPAPAPAPKPEQPAPAPAPKPEQPAPAPKTGWKQENGMW
                     YFYNTDGSMATGWLQ"
BASE COUNT          666 a          270 c          343 g          334 t
ORIGIN      
        1 aagtatagtc taaaaatgac tatcagaaaa gaggtaaatt tagatgaata agaaaaaaat
       61 gattttaaca agtctagcca gcgtcgctat cttaggggct ggttttgtta cgtctcagcc
      121 tacttttgta agagcagaag aagctccaca agttgtcgaa aaatcttcat tagagaagaa
      181 atatgaggaa gcaaaagcaa aagctgatac tgccaagaaa gattacgaaa cggctaaaaa
      241 gaaagcagaa gacgctcaga agaaatatga tgaggatcag aagaaaactg aagagaaagc
      301 gaaaaaagca gaagaagctg ctaaggaagt agatgatgct agtttagcgg tgcaaaaagc
      361 ttatgtggaa caaagaaaag cacaagaaga aaaaaataaa cattcaggaa atccagagta
      421 cgctaaaaaa ttagcagaag cagataaaaa aataactgag gagacgacta aactaaccga
      481 tgctcagaca aaatttcaat ctgttcaagc aatggtagtt cctgagccag agcagttggc
      541 tgagactaag aaaaaagcag aagaagctaa agcagaagaa aaagtagcta agagaaaata
      601 tgattatgca actctaaagg tagtactagc gaagaaagaa gtagaggcta aggaacttga
      661 aattgaaaaa cttcaatatg aaatttctac tttggaacaa gaagttgcta ctgctcaaca
      721 tcaagtagat aatttgaaaa aacttcttgc tggtgcggat cctgatgatg gcacagaagt
      781 tatagaagct aaattaaaaa aaggagaagc tgagctaaac gctaaacaag ctgagttagc
      841 aaaaaaacaa acagaacttg aaaaacttct tgacagcctt gatcctgaag gtaagactca
      901 ggatgaatta gataaagaag cagaagaagc tgagttggat aaaaaagctg atgaacttca
      961 aaataaagtt gctgatttag aaaaagaaat tagtaacctt gaaatattac ttggaggggc
     1021 tgatcctgaa gatgatactg ctgctcttca aaataaatta gctactaaaa aagctgagtt
     1081 agcaaaaaaa caaacagaac ttgaaaaact tcttgacagc cttgatcctg aaggtaagac
     1141 tcaggatgaa ttagataaag aagctgctga agctgagttg gataaaaaag ttgaagctct
     1201 tcaaaacaaa gttgctgatt tagaaaaaga aattagtaac cttgaaatat tacttggagg
     1261 ggctgattct gaagatgata ctgctgctct tcaaaataaa ttagctacta aaaaagctga
     1321 attggaaaaa actcaaaaag aattagatgc agctcttaat gagttaggcc ctgatggaga
     1381 tgaagaagaa actccagcgc cggctcctca accagagcaa ccagctccag ctcctgcacc
     1441 aaaaccagag caaccagctc cagctcctgc accaaaacca gagcaaccag ctccagctcc
     1501 tgcaccaaaa ccagagcaac cagctcccgc accaaaaaca ggctggaaac aagaaaacgg
     1561 tatgtggtac ttctacaata ctgatggttc aatggcgaca ggatggctcc aaa
//
LOCUS       DAVLVF010000152         1609 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVLVF010000152 DAVLVF010000000
VERSION     DAVLVF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.152"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            84..491
                     /locus_tag="VYN66_001966"
     CDS             84..491
                     /locus_tag="VYN66_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1495925.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATPFKEVQKPLENFN
                     HIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
     gene            656..955
                     /locus_tag="VYN66_001967"
     CDS             656..955
                     /locus_tag="VYN66_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV1495926.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            1216..1539
                     /gene="tnpB"
                     /locus_tag="VYN66_001968"
     CDS             1216..1539
                     /gene="tnpB"
                     /locus_tag="VYN66_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1495927.1"
                     /translation="MMIQLSDLGQVHLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
BASE COUNT          552 a          321 c          282 g          454 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact cctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tcaaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattgacta atactcttcg aaaataaaat tcaaaccaca tcagcgtcgc cttaccatac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttt attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcagctag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag gcctctgttg atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataata aaacgcatag
     1021 tagcaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
     1081 caatgaagaa gcaaagatca aactaggcag ctagttacaa gttacgtaag cgcgtcataa
     1141 caaggtatct atcctaattc cccaacttcc agtatactag ataaaaaata attatcggag
     1201 gaaaagtatt gtactatgat gattcaacta agtgatttag gtcaagttca ccttgtttgt
     1261 ggcaagacag atatgataca aggaatcgat tcactggctt atctcgttaa aacccacttt
     1321 gaattggatc ctttctccgg tcaagtcttt ctcttttgtg gtggacgtaa agaccgcttt
     1381 aaagcccttt actgggatgg tcaaggattt tggatactat ataaacgctt tgagaacgga
     1441 aaactgactt ggcccagtac agaaaaggat gtcaaagctc tcgcacctga acaagtagac
     1501 tggcttatga agggcttttc tatcactccc caaatatagt ggatttaaac tagaatagta
     1561 cacccctact tctaaaacat tgttagaaat cgatttgact gtcctgatc
//
LOCUS       DAVLVF010000153         1561 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVLVF010000153 DAVLVF010000000
VERSION     DAVLVF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.153"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(41..1465)
                     /locus_tag="VYN66_001969"
     CDS             complement(41..1465)
                     /locus_tag="VYN66_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1495928.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
BASE COUNT          454 a          354 c          278 g          475 t
ORIGIN      
        1 aaaaaataaa accaggaaaa caatttcctg gttttacacg ttatttcaca tcaaaaacaa
       61 tggatttgac atttgtcatc gcttcaatgc tatatttaat tccttgcact ccagctccag
      121 aacctttgac accaaggaat gggaaattat ctggaccacg ctgggtttta ttattaatgt
      181 ggactgtacc tacttcaagt ttttcagcaa tttcaaaggc ttttttgaaa tcatttgtaa
      241 agactgatga ttgaaggccg aattcagatt cgttggcaaa ggcaatagct tcctctacac
      301 tagccacacg aatgattggt aaaacaggac caaatggctc ttcccatgcc actttcatat
      361 cttttgtaac ttggtcaaaa agcactggcc agagaagatt gccctcacgt ttgattggtg
      421 taagagcctg agcttctttt tcttgtgcat cctcaatcaa gccccaaatg aagtcggctg
      481 aagcattgtc aataacaggt gtaatatcag cattgtcaaa tggatcacca actgttaatt
      541 tagaaacttc ttcctgaagc aaagtagcta atttatctgc tacactttcg agaacaatga
      601 cacgtttaat ggccgtgcaa cgttgtcctg agtagctaaa ggctcccgca acaatttgct
      661 tggcagcatg ttccaaatct gcatcttcta gtacaagagc tgcatctttc ccaccaagtt
      721 ccaacatgat aggacgcata ccagctaaac gaccaatacg ttctccaata ggagttgaac
      781 ctgtaaagtt gatgaagttg acttctttgt gctcaatgat ataatcccca atttctgaac
      841 cacgacctgt aatggtgttg aaaacacctg ccggaatccc tgcttcttca aatgctttag
      901 ccaacaagag tccagaaatg gaaccttgtg ttggtggctt aaacatgacc acattccctg
      961 caatcaaggc aggtgcaatt ttagaagcag ataaattaac tggataatta aagggagcaa
     1021 tcgctagcac gataccaact ggttcacgac ggacaacagc cagtttgttt ttacttgctg
     1081 cctcaaaacc accaccttcc attgcttgtc cagtgatacg gagaccttcc tcagcagcat
     1141 aacgaatcaa gtctgctgta cgcactactt ctccaattgc tgctttaatc ccttttgcta
     1201 cttctttggc aaggatagta ccaatttctt ccttatcgcg ttctaaaata gctgctgttt
     1261 tatgtaaata agccgcacgt tcaactgctg ataaagctcg ccatgctggc agggctgcac
     1321 gcgcagcttg catagcctca tcagcttcag tctgagtcat ggctggaact gtacccaatt
     1381 cttcttgatt gattggtgaa taaatcgtaa tttcttgttc agatgatttc cattttccat
     1441 ttactaaatt ctgatatctt gtcaaattct tgtcctcctg aaaatgatta agatatattt
     1501 taccaaattt tcagaaaatc acaacccttt ttgcaaaatt tttgagatta ttttcacaaa
     1561 c
//
LOCUS       DAVLVF010000154         1555 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVLVF010000154 DAVLVF010000000
VERSION     DAVLVF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.154"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(129..216)
                     /locus_tag="VYN66_001970"
     tRNA            complement(129..216)
                     /locus_tag="VYN66_001970"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Ser,seq:gct)
     gene            complement(223..296)
                     /locus_tag="VYN66_001971"
     tRNA            complement(223..296)
                     /locus_tag="VYN66_001971"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Ile,seq:gat)
     gene            complement(332..402)
                     /locus_tag="VYN66_001972"
     tRNA            complement(332..402)
                     /locus_tag="VYN66_001972"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(368..370),aa:Gly,seq:tcc)
     gene            complement(422..494)
                     /locus_tag="VYN66_001973"
     tRNA            complement(422..494)
                     /locus_tag="VYN66_001973"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(459..461),aa:Phe,seq:gaa)
     gene            complement(498..571)
                     /locus_tag="VYN66_001974"
     tRNA            complement(498..571)
                     /locus_tag="VYN66_001974"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(535..537),aa:Met,seq:cat)
     gene            complement(582..671)
                     /locus_tag="VYN66_001975"
     tRNA            complement(582..671)
                     /locus_tag="VYN66_001975"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(633..635),aa:Ser,seq:tga)
     gene            complement(682..755)
                     /locus_tag="VYN66_001976"
     tRNA            complement(682..755)
                     /locus_tag="VYN66_001976"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(719..721),aa:Ile,seq:cat)
     gene            complement(769..842)
                     /locus_tag="VYN66_001977"
     tRNA            complement(769..842)
                     /locus_tag="VYN66_001977"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Met,seq:cat)
     gene            complement(848..921)
                     /locus_tag="VYN66_001978"
     tRNA            complement(848..921)
                     /locus_tag="VYN66_001978"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(885..887),aa:Pro,seq:tgg)
     gene            complement(937..1010)
                     /locus_tag="VYN66_001979"
     tRNA            complement(937..1010)
                     /locus_tag="VYN66_001979"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(974..976),aa:Arg,seq:acg)
     gene            complement(1020..1105)
                     /locus_tag="VYN66_001980"
     tRNA            complement(1020..1105)
                     /locus_tag="VYN66_001980"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1069..1071),aa:Leu,seq:taa)
     gene            complement(1113..1184)
                     /locus_tag="VYN66_001981"
     tRNA            complement(1113..1184)
                     /locus_tag="VYN66_001981"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1150..1152),aa:Gly,seq:gcc)
     gene            complement(1207..1279)
                     /locus_tag="VYN66_001982"
     tRNA            complement(1207..1279)
                     /locus_tag="VYN66_001982"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1244..1246),aa:Thr,seq:tgt)
     gene            complement(1298..1379)
                     /locus_tag="VYN66_001983"
     tRNA            complement(1298..1379)
                     /locus_tag="VYN66_001983"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1343..1345),aa:Leu,seq:tag)
     gene            complement(1385..1457)
                     /locus_tag="VYN66_001984"
     tRNA            complement(1385..1457)
                     /locus_tag="VYN66_001984"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Lys,seq:ttt)
     gene            complement(1482..1554)
                     /locus_tag="VYN66_001985"
     tRNA            complement(1482..1554)
                     /locus_tag="VYN66_001985"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1519..1521),aa:Asp,seq:gtc)
BASE COUNT          427 a          434 c          339 g          355 t
ORIGIN      
        1 gaaatttgtc ctttctcgag cttagctttt cttcaaccca ctacagttga caaagagcct
       61 taagtttacg gatctgaagt tttattgtct ctaaaaacaa aaaagagagt tacaaactct
      121 cattaaaacg gagaataagg gattcgaacc cttgcgccag ttacccgacc taacgattta
      181 gcaaaccgtc ctcttcagcc tcttgagtaa ttctccaatt aatgggcacg agtggactcg
      241 aaccaccgac ctcacgctta tcaggcgtgc gctctaacca cctgagctac gcgcccaagt
      301 taaaaaactt ggtaatttga acaaagttca aagcgggtga cgagaatcga actcgcgaca
      361 acagcttgga aggctgtagt tttaccacta aactacaccc gcataaatac tatcaataaa
      421 atggcgcgag acggaatcga accgccgaca catggagctt caatccattg ctctaccaac
      481 tgagctaccg agccttattg cgggagcagg atttgaacct acgaccttcg ggttatgagc
      541 ccgacgagct accgagctgc tccatcccgc gttaatataa aaggaggatg tgggattcga
      601 acccacgcac gcttttacac gcctgacggt tttcaagacc gttcccttca gccggacttg
      661 ggtaatcctc caatattcaa atggaccttg taggacttga acctacgacc actcggttat
      721 gagccgagag ctctaaccag ctgagctaaa ggtccgacaa gatcattata gcggcgaagg
      781 ggatcgaacc cccgacctcc cgggtatgaa ccggacgctc tagccagctg agctacaccg
      841 ccatgaatcg ggaagacagg attcgaacct gcgacacctt ggtcccaaac caagtactct
      901 accaagctga gctacttccc gagttaaata gaaaaatgca ccctagagga gtcgaacctc
      961 taaccgcctg attcgtagtc aggtactcta tccagttgag ctaagggtgc tccatattat
     1021 gccgaggacc ggaatcgaac cggtacgatc gttaccaatc gcaggatttt aagtcctgtg
     1081 cgtctgccag ttccgccacc ccggcctctc taagcgaacg acgggattcg aacccgcgac
     1141 ccccaccttg gcaaggtggt gttctaccac tgaactacgt tcgcactgtt ttcttctatc
     1201 taaaaatgcc ggctacatga cttgaacacg cgaccctctg attacaaatc agatgctcta
     1261 ccaactgagc taagccggct catttgttat atcttaatgc gggttaaggg acttgaaccc
     1321 ccacgccgtt aagcgccaga tcctaaatct ggtgcgtctg ccaattccgc caaacccgca
     1381 tatatgaccc gtactgggct cgaaccagtg acccattgat taaaagtcaa ttgctctacc
     1441 aactgagcta acgagtctaa aataacttgc gttatcttaa acggtcccga cgggaatcga
     1501 acccgcgatc ttcgccgtga cagggcgacg tgataaccgc tacactacgg gacct
//
LOCUS       DAVLVF010000155         1535 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVLVF010000155 DAVLVF010000000
VERSION     DAVLVF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.155"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            27..1373
                     /gene="gdhA"
                     /locus_tag="VYN66_001986"
     CDS             27..1373
                     /gene="gdhA"
                     /locus_tag="VYN66_001986"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV1495929.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          475 a          297 c          323 g          440 t
ORIGIN      
        1 aaatatgaca ataaaaggag aacgatatga catctgctaa agaatatatc caaagcgtgt
       61 ttgaaactgt gaaagctcgt aacgggcacg aggctgaatt cctccaagct gttgaagaat
      121 ttttcaacac tttagaacct gtatttgaaa aacaccctga gtatatcgaa gaaaatatct
      181 tggcacgtat tactgagcct gagcgcgtgg tttctttccg tgttccttgg gttgaccgtg
      241 atggaaaaat tcaagtaaac cgtggttacc gtgttcaatt caactcagct gttggaccat
      301 acaaaggtgg acttcgtttc cacccaactg taaaccaagg gatcttgaaa ttcctcggat
      361 ttgaacaaat ctttaaaaat gtcttgactg gacttcctat cggtggaggt aaaggtggat
      421 cagacttcga tcctaaaggt aaaacagatg ctgaagtgat gcgcttctgc caaagcttca
      481 tgactgaatt gcaaaaacac atcggaccat cacttgacgt acctgctggt gatatcggtg
      541 ttggtggacg tgaaattggt tacctttacg gtcaatacaa acgtcttaac caatttgatg
      601 ctggtgtctt gactggtaaa cctcttggat ttggtggtag cttgattcgt ccagaagcaa
      661 ctggttacgg tttggtttac tatactgaag aaatgctcaa agctaacggt aacagctttg
      721 ctggtaagaa agtcgttatt tcaggttctg gtaacgttgc tcaatatgct cttcaaaaag
      781 caactgaact cggtgcaact gttatttctg tttctgactc aaatggttat gtcatcgatg
      841 aaaatggtat cgacttcgat cttttggttg atgttaaaga aaaacgtcgt gctcgtttga
      901 ctgagtatgc agctgaaaaa gcaactgcaa cctaccacga aggtactgta tggacttacg
      961 ctggaaacta tgacattgct cttccatgtg ccactcaaaa cgaaatcaac ggtgaagcag
     1021 ctaaacgttt ggttgctcaa ggcgttatct gtgtatctga aggtgccaac atgccgagcg
     1081 accttgatgc catcaaagtt tacaaagaaa atggtatctt ctacggacct gcaaaagctg
     1141 ccaacgctgg tggtgtagcc gtttcagctc ttgaaatgag ccaaaacagt cttcgcctct
     1201 catggactcg tgaagaagtt gatggccgtc tcaaagacat catgacaaac atcttcaaca
     1261 cagctaaaac gacttcagaa acatacggtc ttgataaaga ctaccttgca ggagctaaca
     1321 ttgctgcctt tgaaaatgta gcaaatgcca tgattgcaca aggtattgtt taagatttat
     1381 ttactcaatc ctcaatcact acgattgggg atttttagct tgaattaaaa aattggaaaa
     1441 tctaattaaa taaaatgaaa tgaaataaaa actgaacaaa ttgattgcgg aattcaaacc
     1501 aatttctaac atatttctag aaattaattt gactt
//
LOCUS       DAVLVF010000156         1493 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVLVF010000156 DAVLVF010000000
VERSION     DAVLVF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.156"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            39..779
                     /locus_tag="VYN66_001987"
     CDS             39..779
                     /locus_tag="VYN66_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495930.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            782..>1493
                     /locus_tag="VYN66_001988"
     CDS             782..>1493
                     /locus_tag="VYN66_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495931.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVII"
BASE COUNT          485 a          193 c          295 g          520 t
ORIGIN      
        1 ggaacacttt tcaaaattaa gtgattggag gcaagaatat ggataaagat tatatattaa
       61 aagtgaaagg gctgtatcat caatttttac taggaaataa taaaacgttg caagtgctga
      121 aaaatgtttc tctttctgct tcgagaggag aatttataag tattctagga attagtggtt
      181 ctggaaagtc aactttatta aaatgtattt ctagtttgct tgaaccaaca agtggggaag
      241 taattttaaa tggaatcaac ccctataaaa tcagaaatgc aaaattgtca agtataagac
      301 gtaacgaagt atcttttata tttcaagcat acaatttaat accttccctg ccggtaatag
      361 aaaatatagc acttcctttg cgattatcac aaaaaaaatt aactattaaa aatgtagaaa
      421 acttactcaa aagaatgaag tttaatgctg gcttaaacga ttttgttgga actctgtctg
      481 gtggagaaca acagaaggtt gctatagcta gagcggttat tgctgatagt gatataatat
      541 ttgctgatga gccaactggg gctttagaca gcgtttctcg tgaagtaatt tttgaattat
      601 tgagagagtt agtaggggcg ggtaagtgtg taattatggt aacgcacgat atagaattgg
      661 cctcgaaaac tgatcgtgca ttaatattga aagatggaaa aattttcaaa gaacttcata
      721 gacctagcgg ggaagagttg tataaaatct tagaggtaca atcaactacg gaggaataga
      781 gatgctcaga ttagtttact atcaattttt acataataag aaacaatggt taggcgtttc
      841 accagtaatt tttgtttcga gtttagttat gggattggct gttaatgggg taattaatgt
      901 tgagaataat tcgcaagtat ttgtcggact tccagatcct aaaccaattt ttatgtttcc
      961 gatagtattc ggaggagtaa cattattctt tgtgctctct aacataataa atatgttggt
     1021 tgaaattttt agagatgatt acgaacttct ggaggtgttg ggagcaagtc tccttcaact
     1081 atcatttctt gtggggggac agatttttat catttcctcg attattagtt ttattgcata
     1141 tctatgctct atatttgtta cgtcaaatta ttattatttt ttacaatact ttttcgggga
     1201 aaatatatta cctgatatcc aatttcaaac gagtgctgtt ggatgtatca ttacagtagt
     1261 gttgatttca tttttagcct ttctaagtgg ttgcttttat acgttcaaga agattagaaa
     1321 taggaagagt tctaaaataa ggcatgttct ttcaatagta aaaagaatac tgctattggc
     1381 tggttttagt gttatctggt tgctttcttt gcaacaaatt ttccaagata gcacaatttt
     1441 agcgaaggcc cagataatat tcaatattgt aatcttagat attgtgatta ttt
//
LOCUS       DAVLVF010000157         1479 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVLVF010000157 DAVLVF010000000
VERSION     DAVLVF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.157"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(171..311)
                     /locus_tag="VYN66_001989"
     CDS             complement(171..311)
                     /locus_tag="VYN66_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495932.1"
                     /translation="MLFYLTKELNSGVKKIGTISTTLVVVAGVSAGVLGGKFDFKFPK
                     KI"
     gene            complement(265..768)
                     /locus_tag="VYN66_001990"
                     /pseudo
     CDS             complement(265..768)
                     /locus_tag="VYN66_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370715.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
     gene            complement(985..>1479)
                     /locus_tag="VYN66_001991"
     CDS             complement(985..>1479)
                     /locus_tag="VYN66_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1495933.1"
                     /translation="LDRKFHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEI
                     LDKLLSYSQDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKD
                     KEKIINALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFV
                     LSRA"
BASE COUNT          456 a          272 c          225 g          526 t
ORIGIN      
        1 attttaagtt tttcttttga atcatcatca aaaaacatct tacataaatc atcgtacaac
       61 gccttactac atacatcagg atcctctcta ttatctaatg aaaaaccttt gggaaaataa
      121 ggatataaat caaatagata atttttaaac ttcatattta ttattctcca ttaaatcttt
      181 ttaggaaatt taaagtcaaa ttttcctcca agcacacccg cacttactcc cgcaactact
      241 acaagtgttg tactaattgt tcctattttc ttcacgccac tgtttagctc ttttgttaaa
      301 taaaacaaca ttgatcatat ctgcttcact tacatacttg taagacaact gttcgttggt
      361 aagatctttt aagagatatt ctttgattgc atctgtatga attttgtagt taatcttaga
      421 aatttctcta ttcaaattcc agcctaatga tagcttagaa ttttaatctg acttaagtct
      481 tttgtaatcc tgaattacat acatcttaaa ttcagcagaa atacataaag caaattcaaa
      541 ggcaatatct gtatgtgcaa atgcaccacc atatcttcca ccttttgaga taattccgat
      601 tgctccagtt ttctctaccc atttcttagg agacaatgta aatgaattat atccagcttc
      661 cgatttaatt cggtcgaatt cgaccgaatt gaaattcatg ttatttaagc ttttccataa
      721 accaagaaac tcaattgtat cttttcttct catccaattt ttaattacat cactcgatac
      781 ttatccgaac gataagctcc acgtaaggtg ttcgcaatcg accaaactgc attgactaca
      841 tttgaaatct gaacatcgtt cgactggaat atcggtagtt tttctttaaa attagacatt
      901 tgcttcccct gtttttatga tttacaattc tcttcattat accacaaaat aggcgctttg
      961 tcaactgtag tgggttgaag tcagctaagc tcgagaaagg acaaatttcg tcctttcttt
     1021 tttgatgttc aaagcgataa aaatccgttt tttgaagttt tcaaagtttc gaaaaccaaa
     1081 ggcattgcgc ttgataagtt tgatgagatt attggtcgct tccagtttgg cattagaata
     1141 gtgtagttga agggcgttga taatcttttc tttatctttg aggaaggttt taaagacagt
     1201 ctgaaaaata ggatgaacct gcttaagatt gtcctcgata agtccgaaaa atttctctgg
     1261 ttccttattc tggaagtgaa aaagcaagag ctgatagagc tgatagtgat gtttcaagtc
     1321 ttgtgaatag ctcaaaagtt tgtctaaaat ctctttattg gttaaatgca tacgaaaagt
     1381 aggacgataa aatcgcttat cactcagttt acggctatcc tgttgtatga gcttccagta
     1441 gcgcttgata gccttgtatt catggaactt tcgatccaa
//
LOCUS       DAVLVF010000158         1443 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVLVF010000158 DAVLVF010000000
VERSION     DAVLVF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.158"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>1443
                     /gene="pcpA"
                     /locus_tag="VYN66_001992"
     CDS             <1..>1443
                     /gene="pcpA"
                     /locus_tag="VYN66_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV1495934.1"
                     /translation="TTAAVILAAYVPNEPILADTPSSEVIKETKVGSIIQQNNIKYKV
                     LTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQAFSYYPD
                     ETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFDFSELEEI
                     ELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLPKSVKTLG
                     SNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKTPKETKEL
                     ASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERLAFYGNLELK
                     ELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEIHIKNKSTEF
                     SVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETDVAKPKKNSN
                     QGVVGWVKDKGLWYYLNESGSMATGWVKDKG"
BASE COUNT          555 a          200 c          243 g          445 t
ORIGIN      
        1 aactacagct gcagttattt tagcagcata tgtccctaat gaaccaatcc tagcagatac
       61 tcctagttcg gaagtaatca aagagactaa agttggaagt attattcaac aaaataatat
      121 caaatataag gttctaactg tagaaggtaa cataggcact gttcaagtgg gtaatggagt
      181 tactcctgta gagtttgaag ctggtcaaga tggaaaacca ttcacgattc ctacaaaaat
      241 cacagtaggt gataaagtat ttaccgttac tgaagtagct agtcaagctt ttagttatta
      301 tccagatgaa acaggtagaa ttgtctacta tcctagctct attactatcc catcaagcat
      361 aaaaaaaata caaaaaaaag gcttccatgg aagtaaagct aaaactatta tttttgacaa
      421 aggtagtcag ctggagaaaa ttgaagatag agcttttgat ttttctgaat tagaagagat
      481 tgaattgcct gcatctctag aatatattgg aacaagtgca ttttctttta gtcaaaaatt
      541 gaaaaagcta accttttcct caagttcaaa attagaatta atatcacatg aggcttttgc
      601 taatttatca aatttagaga aactaacatt accaaaatcg gttaaaacat taggaagtaa
      661 tctatttaga ctcactacta gcttaaaaca tgttgatgtt gaagaaggaa atgaatcgtt
      721 tgcctcagtt gatggtgttt tgttttcaaa agataaaacc caattaattt attatccaag
      781 tcaaaaaaat gacgaaagtt ataaaacgcc taaggagaca aaagaacttg catcatattc
      841 gtttaataaa aattcttact tgaaaaaact cgaattgaat gaaggtttag aaaaaattgg
      901 tacttttgca tttgcagatg cgattaaact tgaagaaatt agcttaccaa atagtttaga
      961 aactattgaa cgtttagcct tttacggtaa tttagaatta aaagaactta tattaccaga
     1021 taatgttaaa aattttggta aacacgttat gaacggttta ccaaaattaa aaagtttaac
     1081 aattggtaat aatatcaact cattgccgtc cttcttccta agtggcgtct tagattcatt
     1141 aaaggaaatt catattaaga ataaaagtac agagttttct gtgaaaaaag atacatttgc
     1201 aattcctgaa actgttaagt tctatgtaac atcagaacat ataaaagatg ttcttaaatc
     1261 aaatttatct actagtaatg atatcattgt tgaaaaagta gataatataa aacaagaaac
     1321 tgatgtagct aaacctaaaa agaattctaa tcagggagta gttggttggg ttaaagacaa
     1381 aggtttatgg tattacttaa acgaatcagg ttcaatggct actggttggg ttaaagacaa
     1441 agg
//
LOCUS       DAVLVF010000159         1425 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVLVF010000159 DAVLVF010000000
VERSION     DAVLVF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.159"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            54..819
                     /locus_tag="VYN66_001993"
                     /pseudo
     CDS             54..819
                     /locus_tag="VYN66_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            835..1314
                     /locus_tag="VYN66_001994"
     CDS             835..1314
                     /locus_tag="VYN66_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495935.1"
                     /translation="MKLNKQKNRMIYVLSNFLYAISVSIIYALNGIVLLVIVSKLGIP
                     GDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYKLKSGLVFGILVALLLFIPNILNTMM
                     MNTSNDLFIKAIELLPFYSLPVFVASNTMSISQYLVVITTIILLYFFTLKKSKKYSF"
BASE COUNT          483 a          178 c          236 g          528 t
ORIGIN      
        1 atggtcattc aatcattctc aaagatataa attttgcact taacaagggt gaaattgttg
       61 gtctagtagg gagaaatgga gttggtaaga gtacgttgat gaaaattctt gttcaaaata
      121 atcaaccgac ttcaggtaat attataaaca gtgataatgt tgggtattta atcgaagaac
      181 caaaattatt tttatctaaa acaggtttag agaatttaaa atatttgtca aatttatatg
      241 gtgttgacta caatcaagaa agatttagaa gtttgatcca agagttagat ttgactcagt
      301 ctattaataa aaaagtaaag acctattctt tgggtacaaa acaaaaatta gctttgcttc
      361 taactctcgt tacggaacct gatatattga ttttagatga accgactaat ggtttagata
      421 ttgaatcatc acaaatagtt ttagcggttc taaaaaaaat tagctttaaa tgaaaacgtg
      481 ggaattttaa tatcgagtca taaattagaa gacattgaag aaatttgtga gagggttctt
      541 ttcttggaga gcgggctttt gacatttcaa aaagtaggaa aagatagtca taatttcttg
      601 tttgagatag ctttttcatc agctacagat agagacattt tcattaccaa acaagaattt
      661 ggggatattg ttcaggaaga gggattgaga attactatgt ctgggaatat tcaaaatagt
      721 gagcttttta aattttttaa cgaaaactct attaaagtag ttgattttga aactaaaaaa
      781 gagacgctta aagatattta cctaaatcgt tcaaaataaa ggaaggttat aatcatgaaa
      841 ttaaataaac agaagaatcg gatgatttac gtcttgtcta attttctata tgccatctca
      901 gtttccatta tttatgcttt gaatggcatt gtgttactag tcatagtaag taaattgggt
      961 attccaggtg atttaggatt agattttata gtagctattg tagtcaatac aattttgtta
     1021 gtcctgtttt attttctatt atcttacatt ttttatttat acaaattgaa aagtggcttg
     1081 gtatttggta ttttagtagc tttactactc tttatcccta atatattaaa tacgatgatg
     1141 atgaatacta gtaatgattt gtttatcaaa gcaattgaac ttcttccttt ttattcttta
     1201 cctgtatttg tggcttcaaa tacgatgtct attagtcagt atcttgtggt aatcactaca
     1261 atcattttat tgtacttttt cactctcaag aaaagcaaga agtattcatt ttagtttcat
     1321 ttagtattat tttgcatacc taaaatacaa taaaaaaatc agtcatcttg gtatgctcct
     1381 gctttcacta ttcaacacgt ttttgactta tactaggctc atttc
//
LOCUS       DAVLVF010000160         1399 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVLVF010000160 DAVLVF010000000
VERSION     DAVLVF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.160"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..740
                     /locus_tag="VYN66_001995"
     CDS             <1..740
                     /locus_tag="VYN66_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1495936.1"
                     /translation="NVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISG
                     FYNVNKGSIKIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDV
                     MRALKLAGCDLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEAS
                     ASIDPDNEFELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELM
                     SKDTRYKSLQEMFNSANEWRVSNERVL"
     gene            724..>1399
                     /locus_tag="VYN66_001996"
     CDS             724..>1399
                     /locus_tag="VYN66_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495937.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKM"
BASE COUNT          525 a          189 c          258 g          427 t
ORIGIN      
        1 agaatgttag ctttgcttat aatgataaag ctgtcattga aaatttatcc tttaatttag
       61 aagaaggaaa gtcctacgca cttgtcggtt catctggatc aggcaaatca acagtagcaa
      121 aacttatatc aggtttttac aatgttaata aaggaagcat aaagataggc gggatagcaa
      181 taagtgaata ttctgacgaa gccttaatta aagccatttc ctttgttttt caagattcaa
      241 aattattcaa gaagagcatt tatgataatg tagctttagc taataaagat gcgacgaaag
      301 atgacgttat gagagcctta aaattagcag gatgcgattt aatattagac aaattcccag
      361 aaagagaaaa tacaatcata ggctcaaaag gtgtttattt atccggtgga gaaaaacaaa
      421 gaattgcaat tgctagagca attttaaagg attccaaaat tattattatg gatgaagcat
      481 cagcatctat tgacccagat aacgagtttg aattgcaaaa agcttttaaa aatcttatga
      541 aggataaaac agttatcatg attgcacaca ggctatctac aattaaagac cttgatgaaa
      601 ttattgtcat ggatagtgga aaaattatag aaagagggtc tgacaaagaa ttaatgtcaa
      661 aagatacaag gtataagagc ctgcaagaga tgtttaacag tgcgaatgaa tggagggttt
      721 caaatgaaag agttttataa aaaaagattt gctcttacag atggaggagc aagaaattta
      781 agtaaagcaa cactggcttc atttttcgtt tattgtataa acatgcttcc tgccatatta
      841 cttatgattt ttgctcagga agttttggaa aatatgggca aaagcaatgg cttttatata
      901 gtattctcag ttttgacttt gatagcaatg tatattttgc tttctatcga atacgataaa
      961 ttatataaca caacctatca agaaagtgca gatttaagaa taaggacagc ggagaattta
     1021 tcaaaattac ctctatctta cttttctaaa catgacattt ccgacatttc acaaacaatc
     1081 atggctgata ttgaaggcat agagcatgca atgagccact caataccaaa ggtgggcggc
     1141 atggtactgt ttttcccatt aatatctgta atgatgctag cgggcaatgt caagatgggt
     1201 ttagctgtaa ttattccatc tattttaagc tttatattta tacctttatc taaaaaatat
     1261 caggttaatg gacagaatag atattatgat gtcttaagaa aaaactcaga aagctttcaa
     1321 gaaaatatcg aaatgcaaat ggagattaaa gcatataatt tatcgaagga tattaaagat
     1381 gacttatata aaaaaatgg
//
LOCUS       DAVLVF010000161         1382 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVLVF010000161 DAVLVF010000000
VERSION     DAVLVF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.161"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(237..1346)
                     /locus_tag="VYN66_001997"
     CDS             complement(237..1346)
                     /locus_tag="VYN66_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1495938.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT          514 a          252 c          203 g          413 t
ORIGIN      
        1 tagaggtgta ctattctagt ttcaatatat tataaatagg tttcattcga actttaatag
       61 actatgaaaa ccagtggaac ttaccctgac acggaattcc actggttttt tcgattttta
      121 tcagattaac ttctactatt cttagctgtg aaaattagtt taagacttta acttattttc
      181 agtcttctaa tctaaaggcc acttccagta aagaaaaatc agggttctcc tgacttttac
      241 ttagctaatt ctctttctcg ttctttcatt attttatgat tttcatacaa acgggataag
      301 aacttagaaa tttctttcaa gatagaatag gttggcactg cgacaatcat tccaactaca
      361 ccatagatat tgcttgacaa caaaagtaaa actaaaatcg tgattggatg aaccttcata
      421 acacttccta caattcgagg atataagata ttgccatcta cctgctgaac aacaagcata
      481 taaatcactg caatcagcag tctatgggga tcagtgaata tatttgcgat gatcatagga
      541 atcaaaccaa tacttggccc cacataagga attaaattgg ctacaccaga aaaaatggca
      601 aaaactaaag catattttaa accaataata ctatagccaa tataagccaa acaacctata
      661 atgattgcgt caatcgaaac tccactaata tagcgagcaa tcgtcgcatt taaattcttt
      721 aataagcctg caatatgcaa gcgatccctc tttagaatcg ttctttcaag catgggcaag
      781 aatttatgtc catctaataa gaaataaacc aaaaaaactg gagtcataat caaaatcaaa
      841 acagtactga taagagctga caagacgctc cccacactat ttgatacgct atttaggata
      901 ttttgaagaa tatcaacata ggataagttt aactgctgaa ttgtagcttc tacatccaaa
      961 ttctggagcg caggataatt agataagtct atgattaagt cttgtactcg actataaata
     1021 gtttgactag atataatcaa actagataac tgattaatca aaataggtaa gagatagaca
     1081 acacctatga ccattcccca gaccaaagta cacaaggtaa ttaaaatacc aagcaaacga
     1141 ttgagtttac agactttatt taagaaagta acaatagggt ttgtcaaata ataaagaaag
     1201 ccccctaata aaaatggaat cataattgta ttaagcacgc taacaaaagg gttaatcaaa
     1261 gatcccatct gtctccatag gtaaaagatg atggttaaga gtaaaatttc tgtggtccaa
     1321 aaaaataatt tatttctacg aaacatgagt tacctccctc actttatttt accatatttt
     1381 ca
//
LOCUS       DAVLVF010000162         1372 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVLVF010000162 DAVLVF010000000
VERSION     DAVLVF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.162"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(1..543)
                     /locus_tag="VYN66_001998"
                     /pseudo
     CDS             complement(1..543)
                     /locus_tag="VYN66_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(619..831)
                     /locus_tag="VYN66_001999"
     CDS             complement(619..831)
                     /locus_tag="VYN66_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1495939.1"
                     /translation="MIQPLLHADETSYRVLENDSHLTYYWTFLSGKAENQAITLYHHD
                     QRRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(840..1145)
                     /locus_tag="VYN66_002000"
     CDS             complement(840..1145)
                     /locus_tag="VYN66_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HEV1495940.1"
                     /translation="MFPVETEEITYKRKKSKGKRQAILAQFDSEEVHHQVEESICPDC
                     QDDLKEIGASLQRQELVFIPAQLKRVDHIQHAYKCQTCSKNNPSDKIVKAPIPKAPL"
     gene            complement(1132..1326)
                     /locus_tag="VYN66_002001"
     CDS             complement(1132..1326)
                     /locus_tag="VYN66_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1495941.1"
                     /translation="MKIIQQQSAIIDSLTNELSLLCEQVAYLTQKLSGKSSEKSVCPF
                     GQLNLFEEESPSEKDGDVPS"
BASE COUNT          378 a          306 c          253 g          435 t
ORIGIN      
        1 tccaacaaac tcataataat agctcttgct ttagctcctt caaaactttg agaaaataac
       61 caccctcgct tttttaaact gagcttaggc taaaagagtc cactggactc ttttactccg
      121 tcccataacc aatgatttaa tggcgcattc agctagatta ttggaaagga ccagatgtcc
      181 gtctttcaaa atagtcttaa aggtttcttt atacttgagg ctatacttga ggctgtattc
      241 aattgccctt cctagttttg aacccgataa aactgactga cgccggcacc aagcaaagaa
      301 atcttccatt aggggttgga gatgttcttg acgtttctgt agtcgttcat cagctggcaa
      361 agcctcccag tttctttcca aggaaaataa ctgattacaa taagctaaac ctttagctcc
      421 taaggatgat ttattcgctt gcttgggggt cgcttcaaaa aacttccttc tcacatgtac
      481 ccaacatcca acaagttcag cttcttccag ttgacaataa gcttctagcg gttcgcagtt
      541 taccaagctt agcgtcgcct tgctcctaaa ccttggactg ctatcgcata ggcagaacta
      601 gaggactaaa gtcctaagtt actgccgcaa catatcacaa tgcacatagc cagaataatc
      661 tcctaggaat tcttgcacta ccgaaccact ccgacgctga tcatggtggt acagcgtgat
      721 tgcttgattc tcagctttcc cagacaaaaa ggtccaatag taggtcagat ggctatcatt
      781 ctctagaacc cgataggagg tttcatctgc atgaagtaag ggctgaatca atagtctcgt
      841 tataaagggg ctttaggaat aggagctttt acaattttat cacttggatt attcttactg
      901 catgtttggc atttgtaagc gtgttggata tgatccactc tttttaattg cgcaggaata
      961 aagaccaatt cttgtcgttg aaggctcgct ccaatctctt ttaaatcgtc ctgacaatca
     1021 gggcaaatgc tctcttctac ttgatgatga acttcttctg aatcaaattg ggcaagaata
     1081 gcttgacgtt tccctttaga tttcttacgt ttgtaggtaa tttcctctgt ttcaactggg
     1141 aacatctcca tctttctcag atggagactc ttcctcaaaa aggttgagtt gtccaaaagg
     1201 gcaaacactt ttctcagagg attttccaga gagcttttgc gttagataag ccacttgttc
     1261 acacagaagg gaaagttcat tagtgagact atcaattata gcactctgtt gttgaatgat
     1321 tttcaatctt ctcccataga ctttcctcct tatttcctaa ctttattata ta
//
LOCUS       DAVLVF010000163         1363 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVLVF010000163 DAVLVF010000000
VERSION     DAVLVF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.163"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(151..339)
                     /locus_tag="VYN66_002002"
     CDS             complement(151..339)
                     /locus_tag="VYN66_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495942.1"
                     /translation="MRLGGSLGQRRENEIFFGLLAILFIKPIIGIVKFFWMIISFAVQ
                     LLFYKILFKILDWLFKLI"
     gene            complement(299..481)
                     /locus_tag="VYN66_002003"
     CDS             complement(299..481)
                     /locus_tag="VYN66_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495943.1"
                     /translation="MNPIKAFDKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIRPEERE"
     gene            complement(483..1262)
                     /locus_tag="VYN66_002004"
     CDS             complement(483..1262)
                     /locus_tag="VYN66_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495944.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLCAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIIAGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
BASE COUNT          449 a          295 c          199 g          420 t
ORIGIN      
        1 aacatatata ctgcaaggtg aagctgacgt ggtttgaaga gattttcgaa gagtataaaa
       61 tgattttact gcttatatta taccatatgg agagagatta aaaaactagc agtggagttc
      121 ctgctagctc tctgcgactt ggattaccat ctagataagt ttaaagagcc aatccaatat
      181 cttaaacaat atcttgtaaa acagcaattg aactgcaaag gaaatgatca tccaaaagaa
      241 tttcacaatc ccaataatcg gtttgataaa aagaatagca agaagaccaa aaaaaatttc
      301 attctctctc ctctggccta atgagccacc gagccgtatc cattagctta tctgcaataa
      361 aaagttttcc cagaccaaca acgacatggt cagctttctt tatcgttttc atcactttta
      421 caccttgcac ggtcaaaaag taattaccat aaattttatc aaaagctttg ataggattca
      481 tattactctc ctttaatgat ttcagcctct tttcggattg tttcaacatc ttcttgatat
      541 tgaacttcac tatagttgac tagcttggat aattctttct gcaagctttc ccccattggt
      601 ttcatagttg caaaggttaa accacctgca atgataccac ccaagatagg aataaatttc
      661 cctattcctt tggccagccc tcccttggta agattcacac caaatatttt taagactttt
      721 ttcaaaatag ggtaccaaag cgtctttgtt aaagctttat taggcactgt tttcattacc
      781 tgtttggcaa ttgttatacc accagcacat agcaaagcag cggttccatt cacccctaac
      841 attacgccta gataaagcaa gagggtattt tgagcatctt cactcaactc ctcgcgtgaa
      901 gcccaaagat cctcataacc ataaatataa cctaattctt gagccaattt cagagagaaa
      961 gcataaaatt gagccacatc agctggaatg gtaattgcca tagcaagccc tccagctaat
     1021 cctgccaaaa cagaagtccc actcgctaat aatacattgt cccgaataca agcattagct
     1081 acacgatcta atatttcttg agatagaaga gtcgttgtac cttgttccaa taaggtagga
     1141 atatcttttg gatccaattc tttggaaaac ttatccacta aaaatttcga acgatcaacc
     1201 ttaacaacag gtagtttcac cacttgttgc aatacttgca tagctaagtc ttgttcagcc
     1261 atatacaaac tccttatttc ttttttacac cttatcatat tatatttcct acaaaaaaag
     1321 cattttctaa ttagataaca ttaaaaaata cagaacccct ttt
//
LOCUS       DAVLVF010000164         1157 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVLVF010000164 DAVLVF010000000
VERSION     DAVLVF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.164"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            49..399
                     /locus_tag="VYN66_002005"
     CDS             49..399
                     /locus_tag="VYN66_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1495945.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWTYLCSQVN
                     QNLEKIRQREGDTH"
     gene            614..730
                     /locus_tag="VYN66_002006"
                     /pseudo
     CDS             614..730
                     /locus_tag="VYN66_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
     gene            723..956
                     /locus_tag="VYN66_002007"
     CDS             723..956
                     /locus_tag="VYN66_002007"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495946.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
BASE COUNT          380 a          227 c          243 g          307 t
ORIGIN      
        1 agttattcac atcttatttc aactcactat agaaggagga ataggaggat tctcagacat
       61 ccgggcatca gcccaactaa tgatttgatt gctaagaaaa tattcagcaa tccagaaatc
      121 acttgtcaat ttattcgcga tatgctggac ttgccagcaa aaaatgtgac cattttggag
      181 ggaagcgata ttcacgtatt actctccatg ccttactcgg tgcaggattt ttataccagt
      241 atagacgtct tggcggagtt ggataacggt actcaagtaa ttattgagat tcaagtccat
      301 catcagaatt ttttcatcaa tcacttgtgg acttacctgt gcagtcaggt taatcaaaat
      361 cttgaaaaaa ttcgtcagcg agaaggtgat actcactaga gctacaaaca catcgctcct
      421 gtttacgcca ttgctatcgt ggatagtaat tatttctcag atgacctggc ttttcatagc
      481 tttagtatgc gcgaagacac aacaggtgag gtattggcga ttaccaacaa tggacaggaa
      541 aaccatctgg ttaagatggc attcttggaa ttaaaaaata cagagaaacc agcaaagaca
      601 aggttcgcaa gccatggttg gagtttttcg gcaacaagcc ctttacccag caaccgcaac
      661 gagccattag ccaagcaaat caactgctgg actacaagag ctggtccgag gaggacagga
      721 aaatgtttag tcaactacat atgcgagaag aacaagtctt gttagcacag gactatgcct
      781 tggaaactgc tagggctgaa ggccttgaac aaggactaga gcgtgggaaa gttgaaggaa
      841 gggcagaaag gaaacttttt gccttcctag acatagtacg ccaaggtctt ctgacttctg
      901 aggttgccag ccagcaatta ggtatgtcag tatctgaatt tgaggcactg ttgtaaaatg
      961 gctccataat atccatagtg ggtaaatccc ctatggatat tatggagcct attttgtgta
     1021 gaaaaaaagt cccatatgac ctataatgaa aagcgacaaa acaactcatt agaaagaatc
     1081 atatggaaca attacatttt atcacaaaat tactagacat taaagaccct aatgtccaga
     1141 ttttaaacat catcaat
//
LOCUS       DAVLVF010000165         1148 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVLVF010000165 DAVLVF010000000
VERSION     DAVLVF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.165"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..650
                     /locus_tag="VYN66_002008"
     CDS             <1..650
                     /locus_tag="VYN66_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEV1495947.1"
                     /translation="MYVYKDEFEGINLESILELEIKNGLEIVYVDENLRSHKKYFYAM
                     KDNPNSIVVLVDDDIIYPRNTIKKLIASYRIYPQCVSAMRCHRIKLFSDGSLYPYNQW
                     EYEISGATIPSYFNFFTSGGGTLFPPCTRNEDLFNKKNIRELSFLADDVWLNFLVVKN
                     GIKTVKATRYKGTPLTIDDNPEESLVYLNTVYDNNNDKCIRNMVEYYQINFTEDK"
     gene            650..>1148
                     /locus_tag="VYN66_002009"
     CDS             650..>1148
                     /locus_tag="VYN66_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEV1495948.1"
                     /translation="MNVEKNLKRGIFYTAIGKYSNVVIQLLVTAILSRILSPVEYGIV
                     AVVNVFLLFFQMLADSGIGPAIVQNKELNKSDLISIFSLTIYSGIVLSCIFALLGYPM
                     GVLYGETIYVSLFPLLGLCVLFYTITIVPQAILMKTMNFKIVNFLTIFSNIASGLVGV
                     ILAVSH"
BASE COUNT          379 a          146 c          207 g          416 t
ORIGIN      
        1 ttatgtatgt ttataaagat gaatttgaag gcattaattt ggagagtatt ctagaacttg
       61 aaatcaaaaa tggtttggaa attgtctatg tggatgagaa tcttcgttca cacaaaaaat
      121 atttttatgc tatgaaggat aatccgaaca gtatagttgt tctagttgat gacgatatca
      181 tttatcctcg aaatactata aagaaactga ttgcatccta tagaatttat ccacagtgtg
      241 tttcagcaat gcgatgtcat cgaattaagt tgttttctga tggaagttta tatccatata
      301 atcaatggga atatgaaata tcaggagcta ctataccatc gtattttaat ttttttacta
      361 gcggaggtgg gacattattt cctccatgta ctcgcaatga ggatcttttt aataaaaaaa
      421 atatcaggga actcagtttt ctagctgatg atgtatggtt gaattttttg gtagtcaaaa
      481 atggaattaa aacagtaaaa gcaacacgat ataaagggac accattaact atagatgaca
      541 atcctgagga gagtttggta tatttgaata ccgtatatga taacaacaat gataaatgta
      601 ttagaaatat ggttgaatat tatcaaatta attttacaga ggataaataa tgaatgttga
      661 aaaaaatttg aaacgtggaa ttttctatac tgcaataggg aagtattcga atgtagttat
      721 tcaattactt gtaacagcaa ttcttagccg gattctctca cctgtagaat atggtatcgt
      781 agcagtagta aatgtatttc ttttattttt tcaaatgtta gcagattcag gaataggacc
      841 tgctattgtt caaaataaag aactgaataa gtcagattta atcagcattt tctcattaac
      901 gatttatagt ggaatcgtac tttcttgtat ttttgcttta ctaggatatc ctatgggggt
      961 gttatacggc gaaactattt atgttagttt atttccgctt ttaggtcttt gtgttttatt
     1021 ttataccatt acaattgtac cgcaagctat tctaatgaaa acgatgaact ttaaaatagt
     1081 taattttcta actatttttt ctaacattgc tagtggcttg gtgggcgtta ttctagcggt
     1141 atcacatt
//
LOCUS       DAVLVF010000166         1142 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVLVF010000166 DAVLVF010000000
VERSION     DAVLVF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.166"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            69..>1142
                     /locus_tag="VYN66_002010"
     CDS             69..>1142
                     /locus_tag="VYN66_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495949.1"
                     /translation="MNLERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRN
                     RVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTL
                     NHYHWSLTVATLFSLMIMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLA
                     SLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIK
                     GIVKIGTIEAIGALTGVIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEP
                     NEVNRDFPLYEAKNICYKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGF
                     LRDYSGELRFCGDDIKKTSYLNMV"
BASE COUNT          392 a          143 c          207 g          400 t
ORIGIN      
        1 ttgctggtca attattgcta atttatgcag caactattaa tgctctggtg ttgaatgaat
       61 taattgcgat gaatttagag cggtttttga aattgtcaat ctaccaaatg attgtctggt
      121 gtgggataat attccttgac tgggtagtga aaaattatca ggttgaagtg atccaagagt
      181 ttaatctaga gattcgaaat agagttgcca cagacatctc taactctacc tatcaagaat
      241 ttcatagtaa atcatcagga acatatcttt cgtggctaaa taatgatgtt cagactttaa
      301 atgatcaggc ttttaaacaa ctttttttag taataaaagg aatttctggt actatatttg
      361 cagttgtgac tcttaatcac tatcattggt cattgactgt agccaccttg ttttcattaa
      421 tgattatgct acttgtacca aaaatctttg catcgaaaat gcgagaagtt agtctaaatt
      481 taactaacca aaatgaagct tttttaaaat ctagtgagac tatattgaat ggatttgatg
      541 tgttagcatc cttgaatctt ttatatgtat tgcctaagaa aattaaagaa gcaggaattt
      601 tattaaagat ggttatacaa agaaagacaa ctgtagaaac gttagcaggc gctattagct
      661 tctttctcaa tatttttttt cagatatctc tcgttttttt aacaggctat cttgcaataa
      721 aaggaatagt gaaaattggt actattgaag caataggagc actaacaggt gttattttta
      781 cagcgctagg tgaattagga ggtcaattat cctctattat tggtacgaag cctatttttt
      841 taaaattgta ttcaattaat ccaattgagt caaataaaat gaatgatatc gaaccaaatg
      901 aggtgaatag agattttccg ttatatgaag caaaaaatat ttgctataag tatggagata
      961 aagaaatatt aaaaaactta aatttttgtt ttcaacgtaa tgaaaagtat ttaattttag
     1021 gtgaaagtgg aagcgggaaa tctacattat taaaattatt gaatggcttt ttgagagatt
     1081 atagtggaga attgcgattc tgcggggatg atataaaaaa aacctcctat ttaaatatgg
     1141 tt
//
LOCUS       DAVLVF010000167         1107 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVLVF010000167 DAVLVF010000000
VERSION     DAVLVF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.167"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(7..>1107)
                     /locus_tag="VYN66_002011"
     CDS             complement(7..>1107)
                     /locus_tag="VYN66_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1495950.1"
                     /translation="SYFLPSFFTVGSMATLFALLSTLVKLFVTFDYIPKVLLQHKVTL
                     IDILVVLFLFFQAFAAFQSQTLYLNYVGGQFFLLGLYSFLKHFLLLDCKTTIKSLFLT
                     FILFLCVQVITQLLFPVGFDSLHPTGDNRLYFLGRKNIATPYIIVGLGGFYLLNKKMN
                     EFISLKEIIFLGLFGILSFLTQSSTAIICYGLFIFIRLLGLKENIGKLYSLVSMAVYV
                     CFSLSIIFSQSTILSTFTAIFSKNATFSGRINIWQLAIRIFEENFWFGRGLNVNFNAW
                     TNGIIVNSAHNTLLDILARTGIFPGVLFIVLLMNLFLGKYRIESKTLLAMLASFMIYI
                     TMETSSMSILLLIIVLCVYWPRGEEKLHEQVT"
BASE COUNT          454 a          180 c          159 g          314 t
ORIGIN      
        1 atggtattaa gtaacttgct catgcaactt ctcctctcca cgtggccaat atacacaaag
       61 aacaataatt agcagcaata tgctcataga tgaagtctcc attgtaatat aaatcataaa
      121 tgaggctaac atagccaaca gtgtttttga ttctattcta tattttccaa gaaacagatt
      181 cataagaagc actataaata aaacacctgg aaatatacca gttcgagcaa gtatatctaa
      241 taaggtattg tgtgctgaat taacgataat cccatttgtc caagcattaa aattaacgtt
      301 gaggcctcgt ccaaaccaaa aattctcttc aaaaatacga atcgctaatt gccaaatatt
      361 gatacgtcct gaaaaagttg catttttgga aaaaatcgct gtaaaagttg ataaaattgt
      421 actttgtgaa aagattatag aaagagaaaa acaaacatat acagccatac tgactaaaga
      481 atatagtttt cctatatttt cctttaatcc caatagacgt atgaaaataa aaagcccata
      541 acatataatc gctgtactgg attgtgttaa aaatgataaa attccgaaca atcctaaaaa
      601 aataatctcc tttagtgata taaactcatt cattttttta tttaataagt aaaaaccacc
      661 taatccaaca atgatatacg gggtagctat gttctttctt cctaaaaagt aaagtctatt
      721 atctcctgta gggtgcaaag aatcgaaacc gaccggaaaa agcaattgag ttattacttg
      781 aacacacaaa aacaagataa aggttaaaaa gaggcttttt attgttgttt tacaatccaa
      841 caataaaaag tgtttcaaaa atgaatatag tcctaaaagg aaaaactgac ctccaacata
      901 gttaaggtac aacgtttggc tttgaaaagc tgcaaatgct tgaaaaaata aaaatagaac
      961 aactagaata tcaatcagtg ttactttatg ctgtaaaaga actttgggaa tataatcaaa
     1021 agttacaaat agcttaacta aagtagaaag caaagcaaat aaagtcgcca ttgatccaac
     1081 tgtaaaaaaa ctaggtaaaa aatacga
//
LOCUS       DAVLVF010000168         1105 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVLVF010000168 DAVLVF010000000
VERSION     DAVLVF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.168"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..>1105)
                     /gene="lanM"
                     /locus_tag="VYN66_002012"
     CDS             complement(<1..>1105)
                     /gene="lanM"
                     /locus_tag="VYN66_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 2 lantipeptide synthetase LanM"
                     /protein_id="HEV1495951.1"
                     /translation="ILSNLENDFSVLEEELGLRGKLNDIKFGKGDTHSQGKTVLILFF
                     DDAKIVYKPKNLIINNSLNTIAEYIRKVDEKIRIRIPRTIAYSDHSYEEFIDYLPLEQ
                     KKKLPEYYYNFGVLLAFIYLFNGSDIHFENLISYGDMPVIIDFETMLQQPLFDDKTGQ
                     SLLDTLFHRVTRTLLLPTEGVKREDGLDVEMSALTGNFKKDAFNGQVLINLNTDKVKF
                     DIGKIDFEGGKNLPVRDGDIEFDKYIKDFKKGFRDFYLIFEELNKTEEFKMLLKANLY
                     GLKTRVLFRDTNSYASVLSFLYHPDFYEEMLDREKALENLWSNKFSNQGIVASECEQM
                     RLLDIPIFYTDTNINEIYDDFGNHFISLHQFSG"
BASE COUNT          372 a          208 c          124 g          401 t
ORIGIN      
        1 tatccagaaa attgatgtag ggaaataaaa tgatttccaa aatcatcgta gatttcattg
       61 atatttgtat ctgtataaaa aatcggaata tctaataacc tcatttgttc acattcgctt
      121 gctacaatac cttgattaga aaacttattg ctccagagat tttccaatgc tttttctcta
      181 tctaacattt cttcataaaa atcaggatga tataaaaaag atagtactga agcatagcta
      241 tttgtgtctc taaaaagtac ccttgtcttt aaaccataca agtttgcttt taatagcatt
      301 ttaaattctt ctgttttatt taactcttca aatatcagat aaaaatccct aaaacctttt
      361 ttgaaatctt ttatatactt atcaaattct atatcaccat cccgaacagg caggtttttc
      421 ccaccttcaa aatcaatttt cccaatatca aactttacct tatcagtatt taaattaatt
      481 aaaacttgac cattaaaagc atccttctta aaatttcctg taagagcact catctcaaca
      541 tcaagaccat cctccctttt tactccctct gttggaagaa gcaaagttcg agtcaccctg
      601 tgaaacaggg tatccagcaa actctgacca gttttatcat caaatagagg ttgctgtaac
      661 attgtttcaa agtctattat tacaggcata tctccatagg aaattaaatt ttcaaaatgt
      721 atatcactcc cattaaataa atatataaat gctaaaagca caccaaaatt ataataatat
      781 tcaggtaatt ttttcttttg ctctagaggt agataatcaa taaattcttc atagctgtga
      841 tccgaataag caatagttcg aggtattctt atcctaattt tttcatcaac ctttcggata
      901 tactcagcaa tagtatttag tgagttattg attattaaat ttttaggctt gtaaacaatt
      961 ttcgcgtcat caaagaagag tatcaaaaca gtttttcctt ggctgtgtgt atcacccttt
     1021 ccaaatttta tatcatttaa tttccccctt agccctaatt cttcttctag cacactaaaa
     1081 tcattttcta aatttgataa aattt
//
LOCUS       DAVLVF010000169         1103 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVLVF010000169 DAVLVF010000000
VERSION     DAVLVF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.169"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>1103
                     /locus_tag="VYN66_002013"
     CDS             <1..>1103
                     /locus_tag="VYN66_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV1495952.1"
                     /translation="NKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEWP
                     LDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNKY
                     KDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTKL
                     LCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKLG
                     TMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEIA
                     KELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKET
                     QLIGGQDNFDEAWDSYLEYINVAEEKEYITKTS"
BASE COUNT          411 a          144 c          205 g          343 t
ORIGIN      
        1 gaataaaaga aaagttagtt tagaagattt ttataaatgg tatagtctaa ataaagaaga
       61 gttattaaat aaggcaactg ttggtgaaaa gtttaatgat aaattaaaag aagagtttct
      121 ccaggaatgg cctttggata ggattttaac aatgtcaatc gatgaatatg taataggaaa
      181 gggacagcaa aataagtctt tatgctacgc tcttgagaag ggaaaataca aaaatctatt
      241 tcttggaatt tctggtggct cagcttcaaa atttggtatt tattggaata aaaaaacaaa
      301 caaatataaa gatcaagcta ataatgagat ttcagagttg gatcagcgat tttcaaaatt
      361 aaaatcagat ttgtatgaaa ttatcaaaga aggtattcgt tttaactttg aaaatcctat
      421 ttttgatatg aaaagatcaa caaatgaatt tattggtcgt tctgctatgg tgacaaaatt
      481 actttgtatc tatactgagg gagatccttt ctttggtgta aatattaata gtcagaaaga
      541 attttggaac cactttgttt ctcagacaaa tcaaggtgga ccttatctgc aaaatcataa
      601 aataattgaa ctagtgtcca aaacttatcc tgagttggag ccatcgaaat taggaactat
      661 gctttttgag tattctaagc tttttatgga aaataaggaa gacaatagta caatggattc
      721 atcaaacaat tttcgtcatc aattaactca atctctatta aagtctccaa acctcatcct
      781 ccgtggtgct cctggcacag gaaagactta tcttgctaaa gaaattgcca aagaattaac
      841 ggatggcaac gaagaccaaa tcggatttgt acaatttcac ccatcctatg attatacgga
      901 ttttgtagaa ggtttaagac cagtatcaaa tggggatgga gctattgagt ttaggctaca
      961 ggacggtatt tttaaagatt tttgtcagaa agcaaaagaa acccaattga ttggaggaca
     1021 agataatttt gatgaggctt gggattctta cttagaatat ataaatgttg ctgaagaaaa
     1081 agaatatata acaaaaacat ctt
//
LOCUS       DAVLVF010000170         1084 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVLVF010000170 DAVLVF010000000
VERSION     DAVLVF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.170"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            91..1002
                     /locus_tag="VYN66_002014"
                     /pseudo
     CDS             91..1002
                     /locus_tag="VYN66_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          355 a          189 c          222 g          318 t
ORIGIN      
        1 ataagatgtg aacaactcta tcaggaaagt caaactaatt tatagaaata ttttagcagc
       61 taaggtgtac tgttatagat tcaatacact atagactgta atcaaacaac gatttggcga
      121 aatgtaaaaa aatatgagga gttcgaactc gactctctcc ttcaagaaac acgtggtggc
      181 cgtaaccatg catatatgac gattgaggaa aagaaagtct ttcttgcccg ccatttgaag
      241 gctacagagg caggagaatt tgttacaatt gatgccttat ttcaggctta taaaaaggag
      301 ttaggtcgtt cctacacacg tgatgccttc tatcaactgt tgaagcacca tggttggcga
      361 aatattatgc cacgtccaga acatcctagg aaagcagacg ctcaaaccat tgtcgcgtct
      421 aaaaataaaa tctcaattca agaagaaaag aaagcgcttt aaaagcagta gacgttttcg
      481 taaggttcgc ttgatgtacc aagatgaggc tggtttcggt agaatcagta aactgggatc
      541 ttgttgggct ccaataggag taggtccaca tgtccatagt cactatatac gagaatttca
      601 ctattgttat ggagctgttg atgcccatac aggcgaatca tttttcttaa tagctggtgg
      661 atgtaatact gagtggatga acgccttttt agaagagctt tcacaagctt atccagatga
      721 ttatctttta ctcgttatgg acaatgctat atggcataaa tcaagtacct taaagattcc
      781 gactaatatt ggttttacct ttattcctcc atacacacca gagatgaacc cattgaacaa
      841 gtgtggaaag agattcgtaa acgtggattt aagaataagg cctttcgaac tttggaagat
      901 gtcatgaatc aacttcaaga tgtcatacaa ggactggaga aggaggtgat aaagtccatc
      961 gttaatcgga gatggactag aatgcttttt gaaagcagat gagtattata tgcaatttct
     1021 ttatataaaa agaccggatt gctccgatct ttcaatagtt catattctca atttctattt
     1081 taaa
//
LOCUS       DAVLVF010000171         1083 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVLVF010000171 DAVLVF010000000
VERSION     DAVLVF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.171"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            119..>1083
                     /gene="pavB"
                     /locus_tag="VYN66_002015"
     CDS             119..>1083
                     /gene="pavB"
                     /locus_tag="VYN66_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV1495953.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQENPELTDALKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLE"
BASE COUNT          370 a          198 c          224 g          291 t
ORIGIN      
        1 taatataagt gttttataag agggatttaa gaaaaatttt aactttttct tagtcctttt
       61 taatttcagg agattatact agagtcatca aataaagaaa gactctaagg agaatcctat
      121 gaaattcaat ccaaatcaaa gatatactcg ttggtctatt cgccgtctca gtgtcggtgt
      181 tgcctcagtt gttgtggcta gtggcttctt tgtcctagtt ggtcagccaa gttctgtacg
      241 tgccgatgtg gtcaatccga ccccaggtca agtcttacct gaagagacat cgggaacgaa
      301 agagggtgac ttatcagaaa aaccaggaga caccgttctc actcaagcga aacctgaggg
      361 cgttactgga aatacgaatt cacttccgac acctacagaa agaactgaag tgagcgagga
      421 aacaaactct tctagtctgg atacactttt tgaaaaagat gaagaagctc aagaaaatcc
      481 agagctaaca gatgccttaa aagaaactgt agatacagct gatgtggatg ggacacaagc
      541 aagtccagca gaaactactc ctgaacaagt aaaaggtgga gtgaaagaaa atacaaaaga
      601 cagcatcgat gttcctgctg cttatcttga aaaagctgaa gggaaaggtc ctttcactgc
      661 tggtgtaaac caagtaattc cttatgaact attcgctggt gatggtatgt taactcgtct
      721 attactaaaa gcttcggata atgctccttg gtctgacaat ggtactgcta aaaatcctgc
      781 tttacctcct cttgaaggat taacaaaagg gaaatacttc tatgaagtag acttaaatgg
      841 caatactgtt ggtaaacaag gtcaagcttt aattgatcaa cttcgcgcta atggtactca
      901 aacttataaa gctactgtta aagtttacgg aaataaagac ggtaaagctg acttgactaa
      961 tctagttgct actaaaaatg tagacatcaa catcaatgga ttagttgcta aagaaactgt
     1021 cgaaaaagct gttaaagata acgttaaaga cagtatcgat gttccagcag cctacctaga
     1081 aaa
//
LOCUS       DAVLVF010000172         1058 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVLVF010000172 DAVLVF010000000
VERSION     DAVLVF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.172"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..559
                     /locus_tag="VYN66_002016"
     CDS             <1..559
                     /locus_tag="VYN66_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1495954.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(507..1052)
                     /locus_tag="VYN66_002017"
     CDS             complement(507..1052)
                     /locus_tag="VYN66_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1495955.1"
                     /translation="MNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT          382 a          166 c          154 g          356 t
ORIGIN      
        1 gaacaaagac gaaacaacct cttatcctat atatgaaatc ccagaagcat ggagatatat
       61 taaatttgct agcctagtta acttcagaat aggaaaaact cctccacgca gtgaagctac
      121 tttttgggga actgaaatac cttgggtatc tatatcagat atgcctatat ctggttatgt
      181 aactaatacg agagaatcta tttctaaatt agcattaaaa tcaaagaaaa tagacatttc
      241 acctaaagga actttattaa tgagttttaa attatctatt ggaaaagttg ccatattgga
      301 tattcctgcc actcataatg aagccattat atcgatattc ccctatgcta ataaagaaaa
      361 tattatcaga gattacttaa tgatattttt gccacttatc tctactttag gtgattcaaa
      421 agatgctata aaagggaaaa cattaaatag taccagtatc tccgaattat taatccctat
      481 ttctaatcat gaagagatga aaagaatcat ttccaaagtt gatttacttt ttcaaaaagt
      541 ttctcaactt tttgagtaat aagttcctgt tcctcaaaag gagctaacgg aattaatagc
      601 tcgcttagtg tagttttagg aatattatat aaagcttgac ctgatagttt agttattgct
      661 ttcaattgtt tataaaataa cggagaggac aagttaaata atagaaattt tgaaataatc
      721 tctgaacttt cgaatggtgt taattggaaa ataaatccac cagccacaac accatcatag
      781 tctttatcga ttcttgcaaa ctttccaata tgttctaaag aggttgatac aggtgttatt
      841 agctgattat gttttaaata aacttgttca gaggagatga attgtgtatc aatgtagtaa
      901 tcattatcca acagagaaaa ttctaaaggc ttaatattac caccacgtat aattctaaca
      961 cctttattat taatgcttaa atcgcccttc ttgtaagaaa gacctgtatt cattgaaaaa
     1021 atatctttta tttttataac aacccaattc ataggtat
//
LOCUS       DAVLVF010000173         1047 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVLVF010000173 DAVLVF010000000
VERSION     DAVLVF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.173"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            35..697
                     /locus_tag="VYN66_002018"
     CDS             35..697
                     /locus_tag="VYN66_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1495956.1"
                     /translation="MNEQLPDEMTLAKQFACSRMTIKKALDLLVSEGLIFRKRGQGTF
                     VLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRIIHFKLEFANEFLAEKLQVALQS
                     PVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDILQKSIYNYIEGKLGLHIASATKI
                     LRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTPFEYSISRHRYDLFEFNSFALRH
                     SS"
BASE COUNT          344 a          167 c          189 g          347 t
ORIGIN      
        1 gattcgtgaa aagattcttt gtaataaata taccatgaac gaacaattac ctgatgaaat
       61 gacattagct aaacagtttg cctgtagtcg aatgacgatc aaaaaagctt tagacttgtt
      121 agtttctgag ggcttaattt ttagaaaacg tgggcaggga acctttgttc tctctcgtgg
      181 cagctcaaaa agaaaattaa tcgttccaga aagagatatc cggggactga caaaaatatc
      241 tgaagatgct cattctacaa ttgactcgag gattattcac ttcaaattag aatttgcaaa
      301 tgaattttta gcagaaaaac tacaggtcgc tttgcagagt ccagtttata atatttaccg
      361 cctgcgtatt attgacggta aaccttatgt tctggaacaa acttatatga gtaccgatgt
      421 tattccaggt attactgaag atattttaca aaaatcgatt tacaattaca ttgaaggaaa
      481 gttaggattg catattgcca gtgctacaaa aatcttacga gcttcttcta gttcagaaaa
      541 tgagcaacat tacttgcagc tccttccaac ggaaccggta tttgaagtag aacaagtggc
      601 ttatttggat aacggaactc cgtttgagta ctcgattagt cgtcatcgct atgatttatt
      661 tgaatttaat tcttttgcat tacgacattc ctcctaggag aaaatgtgaa aatgaagcca
      721 atcttttaca gactctagtt taagaaaaat ttaaaacagg gcaagaaggt cccatctatg
      781 cttaaatggt ttctcttttc taaataagat ggctttaaaa gagtgatcgt tttatccatc
      841 atgttgaaaa atatcttcgt atagcttata gagtaggtac tgaaattgtt cacctgatct
      901 acttcttata gttatttagt tttaaatagt gtttcaaata ttcttacact gacgagaagt
      961 ttttgagtct tttcttgtaa cacatatagt atactgaggt tagaatagta gactgtgact
     1021 tctaacaaat tgctagaaat gaatttc
//
LOCUS       DAVLVF010000174         1036 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVLVF010000174 DAVLVF010000000
VERSION     DAVLVF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.174"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            2..>1036
                     /locus_tag="VYN66_002019"
     CDS             2..>1036
                     /locus_tag="VYN66_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV1495957.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGISSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLE"
BASE COUNT          349 a          146 c          199 g          342 t
ORIGIN      
        1 tgtgaatcag agtaatgaaa tatttttaaa tacaatacag gaaaaaacac actataagag
       61 gtctatctct tcatcatttc aggaattaaa aaaatggaat tctttgatat ttaggagagg
      121 gcgatattgg taccaatttg ataattggag tatacccaat actggtgtaa taaaggaatg
      181 gtatgtcgaa gataatgact ggattcctaa ggttagttct tccagtttag acaattcaaa
      241 ttcacttaaa attgtctttg atgtggaaaa atatactttg ccagatgata gtatcttaca
      301 ggatgttagg atttcatctg atgagagtta tatagttttg gttgtatcct cactgcgtag
      361 cactaattta attggaataa aaaaatatac tatggaagag ttatttatca ttgaagatat
      421 ttcagtagaa agttcgttct atttgggtaa attcggtgtt atgtatactc gctccaaaga
      481 gtacgggagg ccaagtaagt tattctataa atcttttgat agctttacag aagaggaact
      541 gtttgaagaa aacgaatgct cttttcgatt aaaaatagtc cacattgata gcaataattg
      601 ttttgtaaag tcagtagact ttcagaaagg caggattttt ctatacagtt tcgaccggac
      661 gggttttgtt agacactctt acacagaaac ggtagcccca acacctagag atatcgctct
      721 atttagtaca gaaaaagcac agtactttct tgggatctct tcaactgaag aaaagaaaga
      781 tcaactaatt ctaagggaaa cttcttcggg agacagagtt tctattacta taccatatca
      841 agatagggct agaagagttc actgtatagg acgttatatc cttttagact gttcaaacgc
      901 agcgaattcc gtcttttatc ttgctacatt taaaaataat agtttgaggg agttgagtgt
      961 taataagata acatttgatg aaaagacaac actttatgaa aattcatttt ctgatcgtgt
     1021 tcttctgttt ttagag
//
LOCUS       DAVLVF010000175         1027 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVLVF010000175 DAVLVF010000000
VERSION     DAVLVF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.175"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>1027
                     /locus_tag="VYN66_002020"
     CDS             <1..>1027
                     /locus_tag="VYN66_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV1495958.1"
                     /translation="GVQAGAIVEPEKVEALKEYTGKIEQPSAEDTKPNNENTNTPEEM
                     SIQKKSSALINMNFITDSSKVTGVGSATFIAPNVLLTVAHNFINNSTDNTTGEFRGDK
                     SKNVYEWVTPDGQKGTFTANNIHFYNKKDYPKGFIYDLAVIKLPETTGREHVELVKNY
                     SKVNLNDKLNVHGYPARKYTHLKDATVEMEQEYANNTYGVQYQGGNPGMSGGGIFNAN
                     GEVIGVHQNGAQNRSGGLILSPTQLAWIKSIIAGNEIPPVYDELYRHKDEKKDDAKDE
                     KEVIKKLELRNISSVELYSKDGNKYRHVTSLASLPSNAENYFMKVKSENFKDVMLPVT
                     SITNDTK"
BASE COUNT          407 a          143 c          193 g          284 t
ORIGIN      
        1 caggggtgca agccggagcg atagtcgagc cagagaaagt agaagcactg aaagaatata
       61 ctggtaaaat cgaacaacct agtgcagaag atactaaacc aaataatgaa aatactaata
      121 caccagaaga aatgagtatt cagaaaaaat ctagtgcatt aattaatatg aactttatta
      181 cagattcgtc gaaggttact ggagtaggat cagctacttt tattgcacca aatgtacttt
      241 taacagtagc acataatttc attaataatt ctactgataa tactactggc gaatttagag
      301 gtgataaatc taaaaacgta tatgagtggg tgactcctga tggacaaaaa ggaacattta
      361 ctgctaacaa tattcatttt tataataaaa aagattatcc aaaaggattt atctatgatt
      421 tagcagtgat taagctacct gaaacaactg gcagagaaca tgtagaatta gttaaaaatt
      481 attcgaaagt aaatcttaat gacaaactaa atgttcatgg atatccagct aggaaatata
      541 cgcacttaaa agatgctacg gtagaaatgg aacaagaata tgcgaataac acctacggtg
      601 ttcaatatca aggtggaaat cctggtatga gtggtggagg aatatttaat gctaatggtg
      661 aagttattgg tgtgcatcaa aatggagcac aaaatcgctc aggcggattg attttatctc
      721 ctacacaatt agcttggatt aagagtatta tagcaggtaa tgaaattcca ccagtatacg
      781 atgaacttta tcgtcataaa gatgagaaaa aagatgacgc taaagatgaa aaagaagtta
      841 ttaagaaatt agaacttaga aatatatctt ctgtagaact atattcaaaa gatggtaata
      901 agtatcgaca tgtaacttca cttgcttctt taccaagtaa tgcagaaaat tactttatga
      961 aagttaaatc agagaacttt aaagatgtga tgctaccagt tacaagcatt actaatgata
     1021 ctaaaga
//
LOCUS       DAVLVF010000176         1015 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVLVF010000176 DAVLVF010000000
VERSION     DAVLVF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.176"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>1015
                     /locus_tag="VYN66_002021"
     CDS             <1..>1015
                     /locus_tag="VYN66_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1495959.1"
                     /translation="NLDNKIKENVSHLDNRGGNIEHDKDNLESSIVRKYEWDIDKVTG
                     GGESYKLYSKSNSKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGI
                     ISDIQDRLGHGTAVVAQIVGDDNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVD
                     DGNDIINLSTGQYLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNV
                     SNQSDLLKLISSRKKVRKPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYA
                     PAGSTLSLSELGLNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDK
                     YKLK"
BASE COUNT          378 a          121 c          201 g          315 t
ORIGIN      
        1 taatttagat aataaaatta aggaaaatgt ttctcatcta gataatagag gaggaaatat
       61 agagcatgac aaagataact tagaatcgtc gattgtaaga aaatatgaat gggatataga
      121 taaagttact ggtggaggcg aaagttataa attatattct aaaagtaatt ctaaagtttc
      181 aattgctatt ttagattcag gagtcgattt acaaaatact ggattactga aaaatctttc
      241 aaatcactca aaaaactatg tccccaataa aggatattta ggaaaagagg agggagagga
      301 aggaataata tcagatattc aagatagatt aggtcatggt acggctgttg tagctcaaat
      361 tgtaggggat gacaatatta atggagtaaa tcctcacgtt aatattaacg tctatagaat
      421 atttggtaag tcgtcagcta gtccagattg gattgtaaaa gcaatttttg atgctgtaga
      481 tgatggcaat gatattatca atcttagtac tggacaatat ttaatgattg atggagaata
      541 tgaggacgga acaaatgatt ttgaaacatt tttgaagtat aaaaaggcta ttgattacgc
      601 gaatcaaaaa ggagtaatta tagtagctgc attagggaat gactccctaa atgtatcaaa
      661 tcagtcagat ttattgaaac ttattagttc acgcaaaaaa gtaagaaaac caggattagt
      721 agttgatgtt ccaagttatt tctcatctac aatttcggtc ggaggcatag atcgcttagg
      781 taatttatca gattttagca ataaagggga ttctgatgca atatatgcgc ctgcaggctc
      841 aacattatct ctttcagaat taggacttaa taactttatt aatgcagaaa aatataaaga
      901 agattggatt ttttcggcaa cactaggagg atatacgtat ctttatggaa actcatttgc
      961 tgctcctaaa gtttctggtg cgattgcaat gattattgat aaatacaaat taaaa
//
LOCUS       DAVLVF010000177         1011 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVLVF010000177 DAVLVF010000000
VERSION     DAVLVF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.177"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>1011
                     /locus_tag="VYN66_002022"
     CDS             <1..>1011
                     /locus_tag="VYN66_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV1495960.1"
                     /translation="ENYTYYLPKTQQSETGVYTSFKNLVDAMNSNPNGTFRLGATMDA
                     REVELPDGQESYVNNVFHGILVGTNNEKYYAIYNLKKPLFGELNGATVEKLSLKDVNI
                     SAKDDTATLAKEANNNTHIDNVHADGAIAGERSIGGLVSQVNNSTISNSSYTGRITNT
                     YKTVASYQIGGLVGKLSGPRGLIDKSFASIDLSSNATQGDQSIGGIVGAVENSALISN
                     SYAEGNLNNVQRFANVGGVVGNLWDPVGGLEKSGRLSNVLSDVNVTNGNAIAGYNFNS
                     IKANGTYSNKNNKVVNVVQEDDEILTKDSTVQRGEVLEDAQIKEKKATFVSKNTIKTE
                     DF"
BASE COUNT          386 a          129 c          204 g          292 t
ORIGIN      
        1 agaaaattat acatactatt taccaaaaac tcagcaaagt gaaactgggg tatatacatc
       61 atttaaaaat ttagtagatg caatgaatag caatcctaat ggcacattcc gtttaggagc
      121 aactatggat gctcgtgaag ttgaacttcc agatggtcaa gaaagttatg tgaataatgt
      181 attccatgga atattagtag gaacaaataa tgagaaatat tatgctattt ataatttgaa
      241 aaaaccatta tttggggaat taaatggtgc tacagtagaa aaactttcac taaaagatgt
      301 taatatttcc gcaaaggacg atactgcaac acttgcgaaa gaagcaaata acaatactca
      361 tattgataac gttcatgctg atggagcgat agctggggaa cgtagcatag gaggattagt
      421 atcacaagtt aataattcta ctatttctaa cagtagctat acaggtagaa taactaatac
      481 atataaaaca gttgctagtt atcaaatcgg aggtttagta ggcaaacttt ctggtccgag
      541 gggattaata gataagtcgt tcgcatcaat cgatttatct tcaaatgcaa cacaaggaga
      601 ccagtctatt ggtggaatag ttggtgcagt agaaaatagt gcgcttatta gtaatagtta
      661 tgctgaagga aatctaaata atgttcaacg ttttgcaaac gttggtggag tagttggtaa
      721 tctttgggat ccggttggag gattagaaaa atccggtcga ctttctaatg tactaagcga
      781 tgttaatgta acgaatggta atgctattgc aggttataat tttaatagta taaaagcaaa
      841 tggcacatac agtaataaaa ataataaggt tgtgaacgta gtacaagaag atgatgaaat
      901 cttaactaag gattctactg ttcaaagagg agaagtatta gaagatgcac aaataaaaga
      961 gaaaaaagct acttttgtat ctaaaaatac tattaaaaca gaagatttta a
//
LOCUS       DAVLVF010000178          991 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVLVF010000178 DAVLVF010000000
VERSION     DAVLVF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.178"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..164)
                     /locus_tag="VYN66_002023"
     CDS             complement(<1..164)
                     /locus_tag="VYN66_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEV1495961.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGILP
                     SKNEELMKFF"
     gene            complement(177..926)
                     /locus_tag="VYN66_002024"
     CDS             complement(177..926)
                     /locus_tag="VYN66_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV1495962.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
BASE COUNT          342 a          194 c          113 g          342 t
ORIGIN      
        1 caaaaaaact tcataagctc ttcattttta gatggaagaa tgcccttttt taaattattt
       61 atttttacag aatctatatc atatgcaaca actttatatt tagatgcaaa tagtaacgcg
      121 taggccagcc caacatgccc caaaccaatt actgctatat tcataaaact acttccttat
      181 ttcttaatcc aaaatctaat agaataagct gccccattcc ttaaatacaa ctctttaata
      241 ttgtttaaaa gtttttcaac tgatttccag attatcaaaa tctgagattt atagcacaat
      301 attgatgata ttctatcaat ataatgtttt tcatcaagtt cctcttgata catttttaat
      361 tctttagttt ttcccatata actaaccata ctactatcac ttacatatgg gaagtcctca
      421 taatatatta ctttataacg cataaattca agcgcccttc caatactatt cacaaaaaca
      481 tgatcaacat ggtcaccaag tgaaagcgga caatatacga cacatttgtc gtctaaatgc
      541 attaacagct cttttatgat atcattcttt aatgtgtcct cattttttaa ttcactatag
      601 atatgacggt atagaaaatt gccatttcta tctttcctat agagacattc atagtacgat
      661 aagtgtctaa aatcacactg tagacgttca caagctaacc tgtcttcttt cttcctttct
      721 tcaatcggat atttcccaag gttacacaac ttatgaaatt gcttagcaga gggctgtagc
      781 tgttggctca aagggtaacc agaaaatata gtaataacaa gtacaatttc tccttctgaa
      841 gttaattttg aaatataatc accacaggaa aaaattgcgt catctaaatg tggagataaa
      901 aagatatact tagtattgtt actcataacc attccctcta caatttatct aaaaactcac
      961 taagtgtctg attaaattcc acatcatcaa a
//
LOCUS       DAVLVF010000179          941 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVLVF010000179 DAVLVF010000000
VERSION     DAVLVF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.179"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..57)
                     /locus_tag="VYN66_002025"
     CDS             complement(<1..57)
                     /locus_tag="VYN66_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HEV1495963.1"
                     /translation="MKKISKFLPILVLVMDIII"
     gene            137..>941
                     /locus_tag="VYN66_002026"
     CDS             137..>941
                     /locus_tag="VYN66_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEV1495964.1"
                     /translation="MKSKLGITLRKVRKGKQISLCSVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLVLHNDDYTSSESFANLVQYIRKQYSSQSINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSNIIPSDEELLHLTDYLFKIEKWGYYEIILLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSLNKTNKQIVSQLAINCFILSIDKEEFSNCSYLISKIK
                     TLLDNELNFYEQTVFLYATGYFEFKRCQSTSGIEKMKQAIQVFDILGENK"
BASE COUNT          339 a          152 c          131 g          319 t
ORIGIN      
        1 gatgataata tccatcacca aaaccaaaat cggtaaaaat tttgaaattt tcttcatact
       61 tggacttcct ttccgttttt atttattgta caataaataa aaacggattt aatttttgtt
      121 gggaaaatgg gaaattatga aatcaaaact tggcatcaca cttagaaaag ttcgtaaagg
      181 aaaacaaatt agtctatgct ctgtagcaga tgaacatctt tcaaaatcac aaatatctcg
      241 ttttgaacgt ggggagtcag agatatcttg tattcgacta atcaatattt tagacaaatt
      301 acatattact ttggatgagt tccttgtttt acacaatgac gactatacca gttccgaatc
      361 atttgctaat ctagtacagt acattcgtaa gcaatattca tcacaaagta tcaataatat
      421 agcatgtcta ctttctgata cttctgatta cactctaaat tcttttgaga aaacaatggt
      481 aaaatctatt ttacacacaa tggattcgaa catcattcca tcagatgaag agcttcttca
      541 tctgacagac tatcttttca aaattgaaaa atggggctat tatgaaatca ttttattagg
      601 taactgtgta cgcacaatta actacaactc ctacttctta ttaactaaag agatgttaaa
      661 taactatatt tactcatctc tgaataaaac taataagcag attgtctctc agcttgctat
      721 taattgcttc attctaagca ttgataagga agaattttct aattgctcct atttaattag
      781 taagataaag acattgttgg ataatgaact aaacttttat gaacaaacag tttttctcta
      841 tgcaactggt tactttgaat ttaaacgttg ccaatcaacc agtggaattg aaaaaatgaa
      901 acaagccata caagtcttcg atattttagg agaaaataag t
//
LOCUS       DAVLVF010000180          938 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVLVF010000180 DAVLVF010000000
VERSION     DAVLVF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.180"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            83..880
                     /locus_tag="VYN66_002027"
     CDS             83..880
                     /locus_tag="VYN66_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV1495965.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLIHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
BASE COUNT          249 a          158 c          204 g          327 t
ORIGIN      
        1 acttttatga aatacagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaagatt taggagttta ttatggtttc ttcagaattt atctcaaaga ttgaatttgc
      121 ttgcaataag aaagaaagtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat
      181 gttcgcaggt gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat
      241 taataaaatt gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt
      301 ggcctacatt gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc
      361 tggtagtttc ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac
      421 cttgttcaac cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta
      481 tgcgaatctg atacacgata gtttcatctc aggtgttgtt gagatgaagt taggccgctc
      541 aaatgaattg gtcttgcttg aggcgatttt ggcaaatatt ttcgtaaata ttgcgattct
      601 gtcatttatt ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta
      661 catgtttgta ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt
      721 gaaattcagt gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca
      781 ctggggtgtg actttcatcg gaaactttat cggaggaggc ctcttgatgg gtcttccata
      841 tgccttcctc aataaaaacg aagatactta tgtagattaa gaaaatgagc acgattgagt
      901 cgtgcttttt tcattttcaa aataaggtaa tagctatt
//
LOCUS       DAVLVF010000181          921 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVLVF010000181 DAVLVF010000000
VERSION     DAVLVF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.181"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..865
                     /locus_tag="VYN66_002028"
     CDS             <1..865
                     /locus_tag="VYN66_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 2 lantipeptide synthetase LanM"
                     /protein_id="HEV1495966.1"
                     /translation="LKDKSSYTQLYNKMLSAIPPSVSLSKTTFGLTGDIGYFIVLSII
                     EDYDTNTLACLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINTLVRYYRRGIEKER
                     VLRLVLSLGDTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKVTRLKKYKELILQL
                     VPREKIKIHSLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGSMAIINFYIDYMKE
                     MDTSMELESLLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIAYTFLRYIALNQND
                     KKIVIPSLLEI"
BASE COUNT          322 a          118 c          168 g          313 t
ORIGIN      
        1 attgaaagat aaatcatcat atactcaact gtacaataaa atgttatctg caattccgcc
       61 atctgtttct ttatctaaga ccacatttgg tttaactggg gatataggat atttcattgt
      121 tctatccatt atagaggatt acgatacaaa tactctcgca tgcttaaatt atataaagag
      181 tgttttaact gagcttgaaa agactgattt tgcaagtgtt tcaaacaagt ctgactatat
      241 taatggtctt ctacctttaa ttaatacact ggtaagatat tataggagag gaatagagaa
      301 ggaacgtgtt ctaaggctag tattaagttt aggtgatacg ctatataatg atgtaagcga
      361 gagagataat agtaattttg gatatagttt tggccatgga ctatcaggtg taatttttac
      421 attaaaaaat atttataaag tgacaagact taaaaaatat aaagaattaa ttttacaact
      481 agttcctagg gagaaaatca aaattcattc tttgaagtgg tgtagtggag aatttgggac
      541 attagttaat catccccaaa gtgttccaga gggaattgta gctagtttag ataatgatac
      601 attttgccat ggaagtatgg ccataataaa tttttacatc gactacatga aagaaatgga
      661 tactagtatg gaattggagt ctttattgtg tagtgttgta atgaataaat caaaagaagg
      721 agagtatcgt atcgtccaaa caagcaattt cccagatttt tctttgtata caggtgtgac
      781 tggtattgct tatacttttt taaggtatat cgcactgaat cagaatgaca aaaagatagt
      841 tatcccctca ttacttgaaa tctagcttaa tttatagttt tgtttctaaa atagaggtgt
      901 ccactcatta agcaatataa a
//
LOCUS       DAVLVF010000182          919 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVLVF010000182 DAVLVF010000000
VERSION     DAVLVF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.182"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            14..489
                     /locus_tag="VYN66_002029"
                     /pseudo
     CDS             14..489
                     /locus_tag="VYN66_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            527..829
                     /locus_tag="VYN66_002030"
     CDS             527..829
                     /locus_tag="VYN66_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1495967.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEARPILFRIPAMSVPVFDNIRLPAKQNMV"
     gene            837..>919
                     /locus_tag="VYN66_002031"
     CDS             837..>919
                     /locus_tag="VYN66_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEV1495968.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEE"
BASE COUNT          299 a          158 c          185 g          277 t
ORIGIN      
        1 aagggaaaac cttatggtat tagattattt ctttgacaaa aaccttgtgt tttgcttaga
       61 agcggataat caagaataac tctttgatca ggtagcaagc ttgttggagg aacgagaaat
      121 tgtaactcca acttatcgtg aagctttgat cacgcgtgaa aagtcatttc caactggttt
      181 agatatggaa tttctaggaa aggacttgca aatgtaatga ttcctcacac agatattgtt
      241 cataatctag ctgagaaagt ggtggttgtt cgattagaga aaccggtgac tttccataat
      301 atgattgcgc caggtaagga agtagaagta tccttgctct tctttatcat caataattca
      361 agttcaagtc aaacgaacat tcttgctcag ttgatggact ttttcacagg aaatggacat
      421 cttgaagacc tatcaaaaat ttccgaacca gaaaaacttt atgcttatat agatgaagca
      481 atcgcttaat cttgtctatt aaaaaataaa atcggaggaa gtctatatga ttaaaattct
      541 tgctgcctgc ggtgcaggtg ttaactcaag tcaccaaatt aaaagtgctc tagaagaaga
      601 actttcaaac cgtggttacg atgttcactg tgatgcagtt atggtgaaag atgtaaacga
      661 agaccttatg aaaggttacg atatctttac accaatcgct gcaacagacc ttggttttga
      721 accaggtatc ccagttatcg aagctaggcc aatcttattc cgtatcccag caatgagcgt
      781 tccagtattt gacaatatta gacttcctgc gaaacaaaat atggtatagt agttctatga
      841 atgatgaagc aagtaaacaa ctaactgatg cacgatttaa gcgtcttgtt ggtgttcagc
      901 gtaccacttt tgaagagat
//
LOCUS       DAVLVF010000183          919 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVLVF010000183 DAVLVF010000000
VERSION     DAVLVF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.183"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            44..784
                     /locus_tag="VYN66_002032"
     CDS             44..784
                     /locus_tag="VYN66_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495969.1"
                     /translation="MENINIKIPEEKISVIIGSNGCGKSTLIKTLSRLIKPLEGEVLL
                     DNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGRFPYRKPFKSLGKDDLEII
                     NRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSILLLDEPTTYLDISYQIEL
                     LDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKLVAEGKPEDILNDKLVKDI
                     FNLEAKIIRDPISNSPLMIPIGKHHVNS"
BASE COUNT          355 a          152 c          149 g          263 t
ORIGIN      
        1 gtggtcaaat aatcttacct gcggttatga tgaaaaaata atcttggaaa atataaatat
       61 aaaaatacct gaagaaaaaa tatcagttat tattgggtca aatggttgtg ggaaatcaac
      121 actcattaaa accttgtctc gacttataaa gccattagag ggagaagtat tgcttgataa
      181 taaatcaatt aattcttata aagaaaaaga tttagcaaaa cacatagcta tattacctca
      241 atctccaata atccctgaat caataacagt agctgatctt gtaagccgtg gtcgtttccc
      301 ctacagaaag ccttttaaga gtcttggaaa agatgacctt gaaataataa acagatcaat
      361 ggttaaggcc aatgttgaag atctagcaaa taacctagtt gaagaacttt ctgggggtca
      421 aaggcaaaga gtatggatag ctctagccct agcccaagat acaagtatcc tacttttaga
      481 tgagccaact acttacttgg atatctcata tcaaatagaa ctattagacc tcttgactga
      541 tctaaaccaa aaatataaga caaccatttg catgattttg cacgatataa atctaacagc
      601 aagatacgct gattacctat ttgcaattaa agaaggtaaa cttgttgcag agggaaagcc
      661 tgaagatata ctaaatgata aactagttaa agatatcttt aatcttgaag caaaaattat
      721 acgtgaccct atttccaatt cgcctctaat gattcctatt ggcaagcacc atgttaactc
      781 ttaataattg aatattggaa ttcatttaaa agtcgcaaat tttgaactga ccctcaaaag
      841 ttaggccaaa aaactaacta aaggaggtca gttttttaat ggcaaataca gcacagaatt
      901 taagataaaa ttagtaaaa
//
LOCUS       DAVLVF010000184          892 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVLVF010000184 DAVLVF010000000
VERSION     DAVLVF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.184"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..880)
                     /locus_tag="VYN66_002033"
     CDS             complement(<1..880)
                     /locus_tag="VYN66_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1495970.1"
                     /translation="MGDESYSFSTLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWS
                     FGLAGFIFQTMLRNPLASPDIIGVTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSS
                     LILFLLAKKDGFSAARLIILGIGFQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFT
                     KLDDIPLVLIVSIIATILVLFFNKRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLT
                     AFSTSITGPIACISFLAGPIALNIGKKRSPILAGLVGILLVLLSDIFSQNILPARYPV
                     GVVTGLLGSPYLIYLLI"
BASE COUNT          336 a          152 c          132 g          272 t
ORIGIN      
        1 ttattagtaa gtatattaag tatggtgaac ctaacaagcc agttacaaca cctactggat
       61 atctagctgg taaaatattt tgagagaata tgtctgataa caaaactagt aaaattccaa
      121 ccaatccagc taatattggg cttcttttct tgccaatatt taaggctatg ggaccagcta
      181 aaaaagatat acaagctatt ggtcctgtaa ttgaagtaga aaaagcagtt aaagatacag
      241 cgcaaaaaat taaaacaagc cttgaaagct cgggatttgc tccaagtccg attgctattt
      301 cttcaccaag ttcaataatt tctagtcttt tattaaaaaa taaaactaat atagtagcaa
      361 taatacttac tattagaaca agaggtatgt catctaactt tgtaaaagat aaagagccac
      421 tgagccatct cataacttct tgtaattcat atcttgctac tttcaacaat aaaaatgagg
      481 tgcctgctct tgtgacagct tgaaaaccaa tacctaatat tatcagtctt gctgctgaaa
      541 aaccatcttt tttagctagt aaaaataata ttaaagatga tgttagtcca caagttattg
      601 aaataattcc agtagttaaa ctatttgttt ttaataccaa tatgcaaaag accgctgcaa
      661 tagatgaaga acttgtgaca ccgattatat caggacttgc aagaggattt cttaacatag
      721 tttgaaagat aaatcctgcc aatccaaaag accagccagc tataattcct gctaataatt
      781 ttggtaatct aatttccata atcgaaaaac tagctccagg aacagtttca ctatttaaga
      841 ctttaatcaa agttgaaaaa gaataacttt catctccgat aagtaaaatg aa
//
LOCUS       DAVLVF010000185          878 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVLVF010000185 DAVLVF010000000
VERSION     DAVLVF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.185"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            161..430
                     /gene="blpJ"
                     /locus_tag="VYN66_002034"
     CDS             161..430
                     /gene="blpJ"
                     /locus_tag="VYN66_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEV1495971.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            499..747
                     /gene="blpK"
                     /locus_tag="VYN66_002035"
     CDS             499..747
                     /gene="blpK"
                     /locus_tag="VYN66_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HEV1495972.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
BASE COUNT          283 a          106 c          208 g          281 t
ORIGIN      
        1 tattgaaatc ttagactaaa aaataggtac tgctaccttt aagaagatag tagacgtata
       61 tactttttta agaaaatcaa aaagatacta aaaaaaatct atttgtttat tgaatttaag
      121 actttggtaa caaattgaaa ataaaaagga ggtattcatc atgaatacaa aaatgttgtc
      181 acagttagag gttatggata ctgaaatgct tgcgaaagtt gaaggggggt atagctccac
      241 tgattgtcaa aatgcactga ttacaggagt cactacggga attataactg gtggaactgg
      301 agcaggtttg gccactttag gagtagctgg acttgctggt gcatttgttg gagcacacat
      361 tggtgcaatt gggggtggat taacatgctt aggaggtatg gttggtgata aattgggact
      421 aagttggtaa gacttatagt aaaattatga ggattctatt ctgatttatt ttagtttttt
      481 aaaaaaggag tatttgtcat ggatacaaaa atgatgtcac aattttctgt tatggatact
      541 gaaatgcttg cttgcgttga aggtggcgga tgcaattggg gagattttgc caaagcaggt
      601 gttggaggag cagctgtagt tgctgcttta ggttgtgcag cgggtggagt gaaatatggg
      661 aaaattctag gaccatgggg cgctgcaata ggaggaattg gaggagcagt ggtttgtgga
      721 tatttagcct ataccgctac atcataaaaa tgaaagctaa attttatgaa ggctatattc
      781 tttattattc tcttcgcttt tcaaacctat cttatttatc tatccataag tatctctgat
      841 aaaaaacaaa agactattga attaacatca ttgaattg
//
LOCUS       DAVLVF010000186          864 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVLVF010000186 DAVLVF010000000
VERSION     DAVLVF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.186"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..761)
                     /locus_tag="VYN66_002036"
     CDS             complement(<1..761)
                     /locus_tag="VYN66_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1495973.1"
                     /translation="MFSHILGFRLETNLRKRGIDGLEKASFRFFDLNPSGQIRKIIDD
                     NAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGIILLALTIIGGLILGAMMGE
                     QEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFKSFYKAIKDYSKYAYDYSLS
                     CKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLELIMILFLSGVLFVSFMRMM
                     WYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNN"
BASE COUNT          298 a          168 c          118 g          280 t
ORIGIN      
        1 aaattattga cattaccatg cactaatttg tctttttgca tatcttcgta aagcgcctct
       61 aaagtatcta ctgcataatt tccttgagaa atatacatgg agtaccacat cattctcatg
      121 aatgaaacaa agagaactcc tgataaaaat aaaatcatga taagctcaag taaaatcacc
      181 tttgcgctag ctaagctagt cataaaataa actataggaa taattaaaat tgcaatcagt
      241 ccaaaaaata accattgata caaaacataa ggccttttac aagataggga ataatcataa
      301 gcatactttg agtaatcttt tatcgcctta taaaagcttt taaaagactc tacatttgct
      361 ttaaatattt ttacaacttg cattcctctc acgtactcaa cagtttcagc acttagttta
      421 gatagggatt cttggtatat cttcataaat tcttgctcgc ccatcattgc ccctaaaatt
      481 aagccaccaa ttatagtaag agcaagcaaa attatgccaa cccttatact tactataaag
      541 ccaagtgcaa gtacaagtac gggtgtgatt attgcctgag aactatcggg aatcatgtgt
      601 gctaccacct gatgagtttg tgctgcatta tcatctatga tctttcttat ttgaccagat
      661 ggatttaagt caaagaacct aaaacttgct ttttccagac catcgattcc cctttttctt
      721 aaatttgttt caagcctaaa tcccaagata tgtgaaaaca ttcctgagac aaaataaaat
      781 atcgctccac ttgttagtgt aataacagat tttaatgcta tactctctgc accggataag
      841 tttgaattaa ttattaactt atct
//
LOCUS       DAVLVF010000187          814 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVLVF010000187 DAVLVF010000000
VERSION     DAVLVF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.187"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>814
                     /locus_tag="VYN66_002037"
     CDS             <1..>814
                     /locus_tag="VYN66_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1495974.1"
                     /translation="HKEIIAKLDYDAPSCPECGSQMKKYDFQKPSKIPYLETTGMPTR
                     ILLRKRRFKCYHCSKMMVAETSIVKKNHQIPRIINQKIAQKLIEKISMTDIAHQLSIS
                     TSTVIRKLNDFHFKHDFSRLPEIMSWDEYAFTKGKMSFIAQDFEKLDIITVLEGRTQA
                     IIRNHFLRYDRVVRCRVKIITMDMFSPYYDLARQLFPCAKIVLDRFHIVQHLSRAMSR
                     VRVQIMNQLDRKSHEYKAIKRYWKLIQQDSRKLSDKHFYRPTFRMHLTNKEIF"
BASE COUNT          249 a          171 c          143 g          251 t
ORIGIN      
        1 acacaaggaa atcatcgcca aactggacta cgacgcccca tcttgccctg agtgcggaag
       61 tcaaatgaag aaatatgact ttcaaaaacc ttctaaaatt ccttatcttg aaacgactgg
      121 tatgcctact agaattctcc ttagaaagcg tcgattcaag tgctatcact gttcaaaaat
      181 gatggtcgct gaaacttcta tcgtcaagaa gaatcaccaa atccctcgta tcatcaacca
      241 aaagattgct caaaagttaa ttgaaaagat ttctatgact gatattgccc atcagctttc
      301 catctcaact tcaactgtta ttcgtaagct caatgatttt cactttaaac atgatttttc
      361 gcgtcttcct gagattatgt cttgggatga gtatgctttt acaaagggaa agatgagctt
      421 cattgcgcaa gattttgaaa agctcgatat catcactgtt cttgagggta gaacacaagc
      481 tatcattcga aatcactttc ttcgctacga tagagttgtc cgatgtcgcg tcaaaattat
      541 tactatggat atgtttagtc cttactatga cttagctaga caacttttcc cgtgtgctaa
      601 aatcgtgttg gatcgctttc acattgtaca acatcttagc cgtgctatga gtcgtgtgcg
      661 tgttcaaatc atgaatcaat tggatcgaaa gtctcatgaa tacaaggcta tcaagcgcta
      721 ctggaagctc atacaacagg atagtcgtaa actcagcgat aaacattttt atcgccctac
      781 ttttcgtatg catttaacca ataaagagat tttt
//
LOCUS       DAVLVF010000188          807 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVLVF010000188 DAVLVF010000000
VERSION     DAVLVF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.188"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..794
                     /locus_tag="VYN66_002038"
     CDS             <1..794
                     /locus_tag="VYN66_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV1495975.1"
                     /translation="IWTKKDSYDVSIPISLFWKSEDTDELPRRKKCILSVYGAYGKND
                     NSDLDEIMLSIIDAGFIYAIVHVRGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRE
                     NDVIDSKRLGLITSSAGGIIAGAVLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKT
                     EIAEWGDIRAPEVKAYIKSYSPMQNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKK
                     LNSIGVKSLLYLNKAADHGGFTPSDVLLMIDTLNYFFEEVGRNNL"
BASE COUNT          282 a           90 c          172 g          263 t
ORIGIN      
        1 tgatttggac taaaaaagac agttatgatg taagcatacc gatttcttta ttttggaaaa
       61 gtgaagatac ggatgagctc ccaagaagaa aaaaatgtat tctaagtgtc tatggggctt
      121 atggtaagaa tgataattct gatttagatg aaattatgtt atctataata gatgcaggtt
      181 ttatttatgc tatagttcat gtaagaggtg gaggctattt gggtggtgaa tggtatcgct
      241 caggaaaggc gttaaacaaa tggaattcta tcagagattt tattgaggga gtaaattatt
      301 taagggaaaa tgatgtaatt gacagtaagc gattaggttt aataacttct agtgcaggtg
      361 gaataattgc tggtgcggtg ttgaacgagg aaaaaaattt attgcaaagc attctcttat
      421 tttcgccatt tataaatcct tatgatacac ttcagaatcc aaatgatcct ctttctaaga
      481 ccgaaatagc agagtgggga gatattagag cccctgaagt aaaggcttat ataaagtcat
      541 attcgcctat gcaaaatatt gaaaaggcac gagactcaaa tactgttata gttaatattt
      601 tgggtgagaa agacccatat attaataata acgaagtgat agagtggtca aagaaattaa
      661 attctattgg agttaaaagt ttgttgtatt taaacaaagc agctgatcat ggaggtttta
      721 ctccatcaga tgttctctta atgattgata ctttaaatta tttctttgaa gaagtgggaa
      781 ggaataactt atgaattatt taaaatt
//
LOCUS       DAVLVF010000189          798 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVLVF010000189 DAVLVF010000000
VERSION     DAVLVF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.189"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(81..269)
                     /gene="pneA2"
                     /locus_tag="VYN66_002039"
     CDS             complement(81..269)
                     /gene="pneA2"
                     /locus_tag="VYN66_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HEV1495976.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
     gene            complement(276..500)
                     /gene="pneA1"
                     /locus_tag="VYN66_002040"
     CDS             complement(276..500)
                     /gene="pneA1"
                     /locus_tag="VYN66_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HEV1495977.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
BASE COUNT          264 a          160 c           99 g          275 t
ORIGIN      
        1 ttaaaaaaaa acctctctca aaattattag ttacgagaga ggaaaataaa gagcaacttt
       61 tatttttaaa attgaatatt ttattttttc ttgttgtctt tgccacaacg agtctgagct
      121 gaccccaaat atgatgcaat aaaacttaat gtagcagaac atataattgt tgatctaggg
      181 tcagcaccat ctgtgccacc ataaaccaat tgcatttctt caagagataa ttctttcaaa
      241 ctctttccga ttacaaaatc atttttcatt ttaatttacc ccaaacaatc attattgtag
      301 ctaactagcc ccccaatacc agaaacagct gcactaatgc atactttcga gctagctgca
      361 gcagatttca aaataatagg agtaggcgtc caacgtggct ctgcaccatc agatgcacca
      421 taaattaaac tcatttcatc agcagacaat gattttaaac tttttccaca aaatttttcg
      481 tttgaattaa agttggtcat aagtaataac tcctccttat aaaaagttaa ataagttgct
      541 ctatattttt cgttttttca gataaattat aattctctca ttctcgtgca aaatatcggg
      601 ttttaatcag ccttcccaac atcaagacca ttggacttct tttgcgatta cactcgaatc
      661 tccaattgtt tattcttctg aataaactgt tggtaaggaa ttgtattact aaacataaaa
      721 acacctagca atgcaaatgt aactaatttt ttaattccac gtttttttct catgcaatga
      781 aacccctttc tttttcct
//
LOCUS       DAVLVF010000190          712 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVLVF010000190 DAVLVF010000000
VERSION     DAVLVF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.190"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            307..606
                     /locus_tag="VYN66_002041"
     CDS             307..606
                     /locus_tag="VYN66_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV1495978.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          266 a          133 c          120 g          193 t
ORIGIN      
        1 actatttatc caaaaataga tagatgtaac atgtttgcaa acaaagcata cgaaccttta
       61 gtaaaatcat ttccatgaaa ctagaataga gccctcttag caaaaatcat tattttaatt
      121 tatttctaat cactccttga cataaataac tctcaccaat aaaagactat gtcttaaaaa
      181 aatggtataa taaaatcaat acttgggctt gatggctatg ctactaataa caattaggag
      241 agaaaatcag gcacttgtta acaacaagga ttatcccctt gagatgaaag gaactttaga
      301 aatcttatga tgaacatgca aaacatgatg cgtcaagcac aaaaacttca aaaacaaatg
      361 gaacaaagcc aagctgaact tgctgctatg caatttgttg gcaaatctgc tcaagatctt
      421 gtccaagcga ccttaactgg cgataagaaa gttgtcagca ttgatttcaa tccagctgtc
      481 gttgacccag aggaccttga gactctttct gatatgaccg ttcaagccat caactctgct
      541 cttgaacaaa tcgatgaaac taccaagaaa aaactgggtg ctttcgctgg gaaattacct
      601 ttctaaaaac aaggagctag aacaatgctt gtcgataaca aaggctaaga aaggtgcaaa
      661 aatgactcta taatatttgt agtgggtaaa tcccctatag atattatgga gc
//
LOCUS       DAVLVF010000191          695 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVLVF010000191 DAVLVF010000000
VERSION     DAVLVF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.191"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>695
                     /locus_tag="VYN66_002042"
     CDS             <1..>695
                     /locus_tag="VYN66_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEV1495979.1"
                     /translation="TFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVSKILRVKP
                     NGKIILKSTVPFGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVVGDETVEG
                     RKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELDSFAERRS
                     LNAEVVIKGVCLDPRIGNFYNNLSFGFGGYCLPKDTKQLKKEFIEINAPVIEAIDISN
                     TNRKQFIVKQILE"
BASE COUNT          249 a           86 c          120 g          240 t
ORIGIN      
        1 tacattttct gaaattaaaa ataatattga ttattatatt attgcgcttc cgacagatta
       61 tgatgagaaa attggtagtt ttaatacata tgaaatcgaa caaacggtat cgaagattct
      121 gagggtaaaa cctaatggaa agattatttt aaagtcaaca gttccgttcg gcttttcaaa
      181 caaattaaaa aggctgtttg atacaaaaaa tatcattttt gtccctgaat ttttgagaga
      241 aggttgttct atatatgata atttatatcc aagtcgcata gttgttggag atgagacagt
      301 tgaaggaaga aaaattgcag agttgttcct ttcgattagt actcatagta ctgccaatat
      361 taaaaatgtt atgttagttt ctcctactga agcagaagca attaagcttt tttctaacac
      421 attcttagct ctccgtgttg ctttttttaa tgaactagat tcttttgctg agaggagaag
      481 tttaaatgct gaagttgtaa taaaaggtgt ttgtttagat ccaagaattg gaaattttta
      541 taataatctt tcttttggat ttggaggata ttgtcttccc aaagatacta aacaattaaa
      601 aaaagaattt atagaaataa atgccccagt gatagaagcg attgatattt caaacacaaa
      661 tagaaaacag ttcatagtta aacaaatatt ggaac
//
LOCUS       DAVLVF010000192          667 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVLVF010000192 DAVLVF010000000
VERSION     DAVLVF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.192"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..664
                     /locus_tag="VYN66_002043"
     CDS             <1..664
                     /locus_tag="VYN66_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1495980.1"
                     /translation="DYVLKGINVTIKKGETVAIVGESGSGKSTLAKILLGLLEPNIGS
                     IEVDGVEKEEIGQTLYRKIFGAVLQNSTLSYGTLRENLTFGHFVSDEELMTNLNSIGL
                     SNVVKSLPLGLETIIAEEGNNFSGGQQQMILLARCLLSKPSVVVLDEATSSLDNLSQQ
                     ITTSYLSEIGTTKILIAHRLDTIKSADKILVMHNGEIVEIGTHRELLELGGIYKQLYS
                     NN"
BASE COUNT          233 a           84 c          131 g          219 t
ORIGIN      
        1 tgattatgtt ttgaaaggaa taaatgttac tattaaaaaa ggagaaactg ttgctattgt
       61 tggagaatca ggttcaggta agagtacatt agctaaaatt ttattaggtt tattagaacc
      121 taatattggt tcaatagaag ttgatggagt agaaaaagaa gaaattggtc aaacattgta
      181 tagaaagatt tttggagcag tgttacaaaa ttcaacccta agttatggta ccttaagaga
      241 gaatttgaca tttggacact ttgtttcaga tgaagaatta atgacaaatc taaattcaat
      301 tggtcttagc aatgtagtta aatctttacc tcttggatta gagacaatca tcgctgaaga
      361 aggtaataac ttttctggag ggcagcagca aatgatactt ttagctcgtt gtcttttgtc
      421 gaaaccttcg gtagttgttt tggacgaagc aacaagtagt ttagataatt tatctcaaca
      481 aattacaact tcttacttaa gtgaaatcgg taccactaag attttaattg cccatcgact
      541 agatactatc aagtctgcag ataagatctt agtaatgcat aatggtgaaa ttgtagagat
      601 tgggacccat agagaacttc ttgaactagg aggcatttat aagcaattgt attcaaataa
      661 ttagttt
//
LOCUS       DAVLVF010000193          639 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVLVF010000193 DAVLVF010000000
VERSION     DAVLVF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.193"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>639
                     /locus_tag="VYN66_002044"
     CDS             <1..>639
                     /locus_tag="VYN66_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1495981.1"
                     /translation="IVVINNGINVSNSDLDVVGVQDFKKEFCIPNNKKIICYVGRLDP
                     EKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAALKEKICHLKCRDNFRLLGEIYPAT
                     IVYQISDLLYIPSDTEGIPMCVLESMSQGTPVLASNVGGLSEIIEHRVDGFLFEKEDV
                     EGVCACANFLLNDSEYLKYIGENSKSKIRKHFSVQKMFVETMRVYDELLEKS"
BASE COUNT          224 a           58 c          140 g          217 t
ORIGIN      
        1 agatagttgt aataaataac ggaattaatg tatcaaatag cgatttagat gtagtaggtg
       61 ttcaagactt taaaaaagaa ttttgtattc caaataacaa aaaaatcatt tgttatgttg
      121 gaaggttgga tccagaaaaa aggccggata gattccttga atttgcagaa aaattatttt
      181 tagtcagaga agatgtgatt tttattatgg ctggaaatgg tagtatgtgg gctgctttga
      241 aagaaaaaat ttgccatttg aaatgtagag ataatttcag gttacttggt gaaatctatc
      301 cagctactat agtttaccaa atatcggatt tattgtatat tccttctgat acagaaggaa
      361 tacctatgtg tgttttagag tcaatgtcac aaggaactcc ggttttagct agtaatgttg
      421 gcgggttaag tgaaattatt gaacataggg ttgatggatt tttatttgag aaggaagatg
      481 ttgagggagt gtgtgcttgt gctaattttt tactcaatga ttctgagtat ttgaaatata
      541 taggtgagaa tagtaaatca aaaataagaa aacatttttc tgtgcaaaaa atgtttgtag
      601 aaaccatgag agtatatgat gaattattag agaagagta
//
LOCUS       DAVLVF010000194          638 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVLVF010000194 DAVLVF010000000
VERSION     DAVLVF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.194"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>638
                     /locus_tag="VYN66_002045"
     CDS             <1..>638
                     /locus_tag="VYN66_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1495982.1"
                     /translation="GATNARNIGIKNSRGKYISFLDDDDEYMSDRILKLMACFKKSRM
                     KNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLCKKEVLELINGF
                     EKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAHSKENGITGVTQKTINADEEYYNY
                     CRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKCKALKVIKKAREEKIFNE"
BASE COUNT          246 a           67 c          127 g          198 t
ORIGIN      
        1 ggtgcaacta atgccagaaa cataggtatc aaaaattcta gaggtaagta tatatcattt
       61 ttagacgatg atgatgaata tatgtcagat cgaattttaa agttgatggc ttgttttaaa
      121 aagagtagaa tgaagaattt agctttagtt tatagttatg gaataataat ttatccaaat
      181 ggtacacgag aagaggagaa gaccgatttt gttggaaatc ccttgtttgt tcaaatggtt
      241 cacaatatag caggtacgtc attttggttg tgtaaaaaag aggtgctaga attaattaat
      301 ggttttgaga aaatagattc acatcaggac ggtgttgttt tattaaaact acttgctcaa
      361 ggataccaaa ttgatatagt gcgagaattc ttggtgaatt actacgctca cagtaaagaa
      421 aacggtatca ctggagtgac acaaaaaaca attaatgcag atgaagaata ttataattac
      481 tgtaggaaat attttaattt attgagtttc aacgagagaa tattggttac aaagaaatat
      541 tattctttaa acataaagcg gttactatta ataggagaca aatgcaaggc tttaaaagta
      601 atcaagaagg caagagaaga aaaaattttt aacgaatt
//
LOCUS       DAVLVF010000195          629 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVLVF010000195 DAVLVF010000000
VERSION     DAVLVF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.195"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>629
                     /gene="phtB"
                     /locus_tag="VYN66_002046"
     CDS             <1..>629
                     /gene="phtB"
                     /locus_tag="VYN66_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV1495983.1"
                     /translation="LKDVSSDKVKLVDDILAFLAPIRHPERLGKPNAQITYTDDEIQV
                     AKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKE
                     KGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPH
                     YDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGF"
BASE COUNT          200 a          129 c          139 g          161 t
ORIGIN      
        1 actcaaggat gtctcaagtg ataaagtcaa gttagtggat gatattcttg ccttcttagc
       61 tccgattcgt catccagaac gtttaggaaa accaaatgcg caaattacct acactgatga
      121 tgagattcaa gtagccaagt tggcaggcaa gtacacaaca gaagacggtt atatctttga
      181 tcctcgtgat ataaccagtg atgaggggga tgcctatgta actccacata tgacccatag
      241 ccactggatt aaaaaagata gtttgtctga agctgagaga gcggcagccc aggcttatgc
      301 taaagagaaa ggtttgaccc ctccttcgac agaccatcag gattcaggaa atactgaggc
      361 aaaaggagca gaagctatct acaaccgcgt gaaagcagct aagaaggtgc cacttgatcg
      421 tatgccttac aatcttcaat atactgtaga agtcaaaaac ggtagtttaa tcatacctca
      481 ttatgaccat taccataaca tcaaatttga gtggtttgac gaaggccttt atgaggcacc
      541 taaggggtat actcttgagg atcttttggc gactgtcaag tactatgtcg aacatccaaa
      601 cgaacgtccg cattcagata atggttttg
//
LOCUS       DAVLVF010000196          619 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVLVF010000196 DAVLVF010000000
VERSION     DAVLVF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.196"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            178..396
                     /locus_tag="VYN66_002047"
     CDS             178..396
                     /locus_tag="VYN66_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV1495984.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
BASE COUNT          207 a           73 c          115 g          224 t
ORIGIN      
        1 gtttgctctt tggttttcat tgagtataac acattgttag aagttggttt aaatttccta
       61 atcagtttgt tcacatttac cttcgatata ttatatccca tagttaaggt tggtcataca
      121 gatgattata gccatggagc cgtaaaactt agtgtttctt tagttgacaa agatgccatg
      181 aaaaaaatat ctgtaactgt aataggatat tttgaaataa atatagatga aaatatcacc
      241 gatattctat acgtaaatgg tactgctatt ctttatcctt atttacgttc tattgtttca
      301 atagtttcgg caattgatag cagtgaagca atgttgctac ctaccattaa tgttttagag
      361 ttactagata aatctcaacc ttttgaagaa gaataattta ttagctcact aaattgaggg
      421 taaggaaaag taaaagcagt aagaaaaatg tcttgcatta tacagcaact ttttgggaat
      481 gagtagatgg attgaataaa atttgattaa gagtggatga tttatctgta gattattatt
      541 ggacagttag tcttgaagta gtctaagaat taggttataa tcagtagaag ccttgctaat
      601 aatgaggagg ttagtttat
//
LOCUS       DAVLVF010000197          617 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVLVF010000197 DAVLVF010000000
VERSION     DAVLVF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.197"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(170..>617)
                     /locus_tag="VYN66_002048"
     CDS             complement(170..>617)
                     /locus_tag="VYN66_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1495985.1"
                     /translation="RNKNGQADTNQTEKPNEEKPQTEKPEEDKEHDEVSEPTHPESDE
                     KENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEK
                     VTDSSIRQNAVETLTGLKSSLLLGTKDNNTISAEVDSLLALLKESK"
BASE COUNT          143 a          130 c           97 g          247 t
ORIGIN      
        1 tcgatttcta acaatgtttt agaagtagag gtatactatt ctagtttcaa tctactatat
       61 tttgcacatt ttcatgaaaa aaatgagaac tagaactcac attctgctct catttttcgt
      121 tttcccgttc tcctatcctg tttttaggag ttagaaaatg ctgctacctt tacttactct
      181 cctttaataa agccaagaga ctatctactt ctgctgaaat agtgttatta tctttcgttc
      241 cgagaagaag actacttttt agaccagtca atgtctccac agcattttgt ctaatactag
      301 aatctgttac tttttctagc aaggcctccg catctgctat cttagcgtta ataacagaat
      361 gctctacttg aggaatttca gcctcatctg tagtatcttc agcttcttcc tctgtctctt
      421 ccgtatcagt gcttggttta taaagattat ctgctgaagg atttaaacca gcgtgattct
      481 ctttttcatc agattcaggg tgagttggct cacttacttc atcatgttcc ttatcttcct
      541 caggtttttc tgtctgaggt ttctcctcgt ttggtttttc cgtttgattg gtatcagctt
      601 gaccattttt gtttctt
//
LOCUS       DAVLVF010000198          612 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVLVF010000198 DAVLVF010000000
VERSION     DAVLVF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.198"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>612
                     /locus_tag="VYN66_002049"
     CDS             <1..>612
                     /locus_tag="VYN66_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1495986.1"
                     /translation="YLKEGGEYQIKNLSYKFTDDECLSLKDISFKLGKIYGIIGSNGR
                     GKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRL
                     GVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFIFFDEPSS
                     GMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADEIFE"
BASE COUNT          239 a           69 c          115 g          189 t
ORIGIN      
        1 ttatttaaaa gaaggtggag agtatcagat aaaaaatctt agctacaaat ttactgatga
       61 tgagtgttta agcttaaaag atatttcatt caagcttggg aaaatttatg gcataatagg
      121 atccaacgga cgaggaaaat caacgctttt aagatgttta ataggtcttg agaaaaaatc
      181 aaaagaagaa atttatttta agggagagaa gctatctaaa aaagaaagac tcaaaaactc
      241 ttcacttgtt atgcaagatg taaatcatca attattcaca gatgaagtat tcaacgagct
      301 tagattagga gtaaagaatt ttgatgaaga aaaggcgaaa atcattttaa aagatttagg
      361 cctggacgaa tttattgaaa ggcatccgat gagtttatca ggagggcaaa agcaaaggct
      421 tgcaatagca tctgttatgt gcaagaattc tccatttatc ttttttgacg aaccttcaag
      481 tggtatggat tattccaata tgataaaaat atctgaactg attaataagt ataaaaccat
      541 ggataaaata atttttattg tttcccatga tatagaattt ttaaatgaag tggcagatga
      601 aatttttgaa tt
//
LOCUS       DAVLVF010000199          607 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVLVF010000199 DAVLVF010000000
VERSION     DAVLVF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.199"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(222..518)
                     /locus_tag="VYN66_002050"
     CDS             complement(222..518)
                     /locus_tag="VYN66_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEV1495987.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
BASE COUNT          215 a          109 c           77 g          206 t
ORIGIN      
        1 tttatttttt tactttggat aggcagtttc atattataac gattcaaagt agcgtaatct
       61 actaccaaaa aaagtaaaaa taatttccct tgaaaaggaa gatagctttt aaaatagatt
      121 gacaaactta tgataacaaa aaacaaaagg gttatcccaa taaaattaag ataacctaaa
      181 atactttcac attctttttt agaaagtatt ttcatctagt tctaccatag agctgcttta
      241 gcagcacctt tctttttcaa tatataaaga actgttgctg caatacccga accaatagaa
      301 cctactcctg tgattgcacc tacaattgaa attactgctg taactgtact ataagcacta
      361 atcaaattga ctacagtagc agcagctttt gtcgaaattc ctaatgtccc agccaacata
      421 ggaaaagaaa caacacctaa gcttaaccaa ctaatgactg ctaacattgc tgaaacaata
      481 agaaatttat tatgaaattc cattctttta gatttcattt tagtgtactc cttttgtata
      541 ttatgtatgt tttattgaga gttaattcgc acatgttgta ttcctaactc tcttcaaatg
      601 ttcgtct
//
LOCUS       DAVLVF010000200          587 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVLVF010000200 DAVLVF010000000
VERSION     DAVLVF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.200"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            6..497
                     /locus_tag="VYN66_002051"
     CDS             6..497
                     /locus_tag="VYN66_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1495988.1"
                     /translation="MLSQNLGVVPQDSFLLNRSILDNITLKHEVTSQKIEEVCKAVQI
                     YDEIMAMPMKFNTIISEMGSNISGGQRQRIALARALINNPSIVILDEATSALDTINEE
                     RITKYIQSQGCTQIIVAHRLSTIKDADVIFVMKGGKIVESGNHKYLMDLGGEYYSLYT
                     KRK"
BASE COUNT          224 a           68 c          123 g          172 t
ORIGIN      
        1 aaaaaatatt aagtcaaaat ttaggagtag ttccacagga ttcattttta ttgaaccgaa
       61 gtattcttga taatataact ttaaagcacg aagttacttc acaaaagata gaggaagttt
      121 gtaaagcagt tcaaatctat gatgaaatca tggctatgcc gatgaaattt aatactatca
      181 tctcagagat ggggtcaaat atttcaggtg ggcaaaggca acggatagca ctggcacgtg
      241 cattaataaa taatcctagt attgtaattt tagatgaagc aactagtgca ttagacacta
      301 ttaatgagga aagaataaca aagtatatac aaagtcaggg ctgtactcaa ataattgtag
      361 ctcatagatt gtcaacgatt aaggatgcgg atgttatttt tgtaatgaaa ggtggtaaga
      421 ttgttgagtc aggaaatcat aagtacttaa tggatcttgg tggagagtac tacagcttat
      481 atacaaaaag gaaatgaggt gtaaagaaaa tgaagaaaga aaatgaatat gtaattttaa
      541 caacagcctc actaggagtg atgattggaa tagtgtttgc aattttt
//
LOCUS       DAVLVF010000201          574 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVLVF010000201 DAVLVF010000000
VERSION     DAVLVF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.201"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            1..424
                     /locus_tag="VYN66_002052"
                     /pseudo
     CDS             1..424
                     /locus_tag="VYN66_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            419..>574
                     /locus_tag="VYN66_002053"
     CDS             419..>574
                     /locus_tag="VYN66_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1495989.1"
                     /translation="MESLSDDDYDFAFDEALFRFSILNANHFLSKEDIDYLRVNLPRS
                     TAEEINNY"
BASE COUNT          219 a           86 c           81 g          188 t
ORIGIN      
        1 tgacaattta gatattccgt tcgaatttaa actaacaaaa gaagcacaaa atcttattat
       61 cgaacatagt gactatatca aagcatacta tgataaagac ataactatgg aagattattg
      121 taaagaaaaa aacaatatct cttttaaagc aacttttggt gatataaaat ggagtctctt
      181 aaataaacaa gctcatagga atactgtatc aggagatttt ggatgcttat ctaacacacg
      241 aaaggctcag ggaagacatt tggaacaaga aggtaatatt aaacatgctt taatatatta
      301 catagaatct ttgataatta ctatttcagg attagaaaac aatttttcag ccactgatta
      361 tccagtatat tatcccgatt cgatacctga ctactcacta aaacatattc aaacattaat
      421 ggaatcatta tctgatgacg attatgattt tgcttttgat gaagcattat ttcgcttctc
      481 aattttgaat gcaaatcatt ttttatctaa ggaagatatt gactatttaa gagttaattt
      541 acctcgttcc actgctgaag aaataaacaa ttac
//
LOCUS       DAVLVF010000202          562 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVLVF010000202 DAVLVF010000000
VERSION     DAVLVF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.202"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..429
                     /locus_tag="VYN66_002054"
     CDS             <1..429
                     /locus_tag="VYN66_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1495990.1"
                     /translation="ELANDSGKKKCHAMKAQAIVTSQGRIVSLDITVNYCRDMKLFKM
                     SCRNIGQAGKILADSGYQGLMKIYPQVQTPRKSSKLKSLIAEDKAYNHALSKERSKVE
                     NIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
BASE COUNT          193 a           98 c          106 g          165 t
ORIGIN      
        1 gaattagcga atgattctgg taaaaagaaa tgccacgcta tgaaggctca agcgattgtc
       61 acaagtcaag ggagaattgt ttctttggat atcactgtga actattgtcg tgatatgaag
      121 ttgttcaaaa tgagttgcag aaatatcgga caagctggta aaatcttggc tgacagtggt
      181 tatcaagggc tcatgaagat atatcctcaa gtacaaactc cacgtaaatc cagcaaactc
      241 aagtcgctaa tagctgaaga taaagcctat aaccatgcgc tatctaagga aagaagcaag
      301 gttgagaaca tctttgccaa agtaaaaacg tttaaaatgt tttcaacaac ctatcgaaat
      361 catcgtaaac gcttcggatt acgaatgaat ttgattgctg gtattatcaa tcatgaacta
      421 ggattctagt tttgcaggaa gtctaatgta attctcaagc tgtgagttca ttatgatttg
      481 taaatccgat atatcttcaa cagctaaaaa cacattttga aatgttccaa gtccgttttc
      541 accaaaacta tattctctct ta
//
LOCUS       DAVLVF010000203          554 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVLVF010000203 DAVLVF010000000
VERSION     DAVLVF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.203"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>554
                     /locus_tag="VYN66_002055"
     CDS             <1..>554
                     /locus_tag="VYN66_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEV1495991.1"
                     /translation="LQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKIL
                     HYHKQKFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNF
                     KPAGKSIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKT
                     AESVNQALTLLLGEHRILSITADN"
BASE COUNT          195 a          100 c          110 g          149 t
ORIGIN      
        1 tttacaacaa gtgagaaaag ggctatacaa aaaggtctat tctgccgatt acgcacaaac
       61 tgtttaccaa ttcaatcgaa aacggtcggt gaaaaagtta attttaacaa aggaaatcag
      121 agagaagatc ttacactatc ataagcaaaa attttcgcct gaaatgatgg ttaacaagaa
      181 gcaagtgaaa gttggtattt caaccatcta ctactggttt cataatggtc atttaggatt
      241 gacgaaggcc gacatgcttt atcccagaaa aaggaaaggt gtcaagaagc aagctagtcc
      301 gaactttaag ccggcaggta aatctatcga agaacgtcct gacgttatta atcttcgctt
      361 ggaaaatggt cattatgaaa ttgataccgt cctactgact aagataaaaa attattgcct
      421 gttagtctta accgaccggc ggagtagaca ccaaattata aggttaattc caaataaaac
      481 tgctgaatct gtcaatcagg cgcttacgtt actattaggg gagcatcgta ttctgtccat
      541 tactgcagat aatg
//
LOCUS       DAVLVF010000204          509 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVLVF010000204 DAVLVF010000000
VERSION     DAVLVF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.204"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..>509)
                     /locus_tag="VYN66_002056"
     CDS             complement(<1..>509)
                     /locus_tag="VYN66_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEV1495992.1"
                     /translation="SPSDLGYYDKAYQLSLYPNQILSQVVTPALHPIMSNFQDNISKI
                     GEVYLQISRIFVIVGIPISAYLYFNAQYVVTFMFGDNWSQSVPVFQILAATIWLQMAN
                     SPTGAFYQATNQTKLLFRIGLLTSFINILAIVIGVMLQSIQCVAYMLLISFSLSLVIN
                     TLYLTKKVL"
BASE COUNT          197 a           81 c           81 g          150 t
ORIGIN      
        1 taatactttt tttgttaagt aaagagtatt gattactaaa ctaagactga aagaaatcaa
       61 aagcatgtac gcaacacatt ggatgctttg caacattaca ccaataacaa tagctaaaat
      121 attaataaaa gatgtcaata agccaattct aaacaataac tttgtctgat tagttgcttg
      181 gtaaaatgca ccagtagggc tattagccat ttgtaaccaa attgttgctg ctaatatttg
      241 aaacaccgga acgctttggg accaattatc tccaaacata aatgtaacaa catattgagc
      301 attaaagtat aaataagccg aaataggtat gccaactatt acgaatattc tagaaatctg
      361 aagataaact tcgcctatct tactgatatt atcttgaaaa ttggacataa taggatgcaa
      421 tgctggtgtc acaacctgtg ataaaatttg attagggtac aaagataatt gataagcctt
      481 atcataataa ccaagatctg acgggctaa
//
LOCUS       DAVLVF010000205          498 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVLVF010000205 DAVLVF010000000
VERSION     DAVLVF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.205"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..369)
                     /locus_tag="VYN66_002057"
     CDS             complement(<1..369)
                     /locus_tag="VYN66_002057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495993.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQYLLYRLL"
BASE COUNT          213 a           83 c           60 g          142 t
ORIGIN      
        1 caataatcgg tataaaagat actgactcat accttgatac caaataaaat tcggtatttc
       61 aataagagca tgaggtaata ttccttttat tgtagctact attccaatca tctgaccaga
      121 tatacccaat tgtattccta aagatagcaa atcaaaaagt tgcaataagg gcgaaatcgg
      181 aaaacagaat agatatagca aaaaatttgt agaatttgcc tgtaaaactg tcaatgtaaa
      241 ttgtaattcc agtttcggag gtatagtagc aaaagtctca ttttttaata taaagcttaa
      301 aagtaaacct agtaaaaaac agactataca aaataacaca tagtaaatca gaaaaccact
      361 ttttctcatc taatgccccc tactaaaatt acaaatatta tcagaaaaca atataaaacc
      421 agaaaactcc atctcaataa actaactgta cctttaatta aaaatagagc tttcttataa
      481 tagacatagg ctataaaa
//
LOCUS       DAVLVF010000206          498 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVLVF010000206 DAVLVF010000000
VERSION     DAVLVF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.206"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..390
                     /locus_tag="VYN66_002058"
     CDS             <1..390
                     /locus_tag="VYN66_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1495994.1"
                     /translation="AYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNILDY
                     YVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDREDLT
                     VIIVTHAPHPELKQYFTKIYQFPKDFI"
BASE COUNT          179 a           51 c          106 g          162 t
ORIGIN      
        1 gcttatttgt ttgaaggtac gattagagat aatattttat tggaagaaaa ttatactgat
       61 gaagaaatac tacagtcttt agagcaagtt ggtttgagtg taaaagattt tcctaataac
      121 attttagatt attatgttgg tgatgatggg agattactgt caggagggca gaaacaaaaa
      181 attactttag ctagagggct aattagaaat aagaaaatag tattaattga cgagggaact
      241 tctgctatcg ataggagaac ttcgttagcg attgaacgta agatattaga tagagaggat
      301 ttgactgtca ttattgttac ccatgctccg catccggaac ttaaacaata ttttactaag
      361 atatatcaat ttccaaagga ttttatttaa gaatataggg gagggaaaca tgagattcta
      421 ttgtgaagct tatgaggtaa ttactgaaga gaaaaaagta tactttaatg atattgagtt
      481 agaagttaag tattcctc
//
LOCUS       DAVLVF010000207          490 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVLVF010000207 DAVLVF010000000
VERSION     DAVLVF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.207"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            41..259
                     /locus_tag="VYN66_002059"
     CDS             41..259
                     /locus_tag="VYN66_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1495995.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
BASE COUNT          214 a           54 c           92 g          130 t
ORIGIN      
        1 ataattaaat aaatctaatg aagaaaaaaa gagggatatt atggcactta actataaacc
       61 attatggata cagttagcaa aaaaaggact aaagaaaaca gatgtaatag ctatggcagg
      121 acttacaaca aatgttatgg cacaaatggg aaaggataaa ccaattacat ttaagaattt
      181 agaaagaata tgtaaggctt tatcttgcac tcctaatgat attattagtt ttgaagataa
      241 ttttagtgac gaggaataga aaatgacttt aaggacagaa gatcaagtta gggattatgc
      301 aagagaagta taggctttaa tgaagttgaa gaaaacatca atcaaggtac tggtcaaata
      361 actactttta atcaattagg cttcaaggga tattcagata agccagatgg ttggtattta
      421 cctaaaaata tgaatgatgt agcaataatc cttgaaacaa aatcagaaga aagagatatt
      481 agcaaacaaa
//
LOCUS       DAVLVF010000208          467 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVLVF010000208 DAVLVF010000000
VERSION     DAVLVF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.208"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..328)
                     /gene="tnpB"
                     /locus_tag="VYN66_002060"
     CDS             complement(<1..328)
                     /gene="tnpB"
                     /locus_tag="VYN66_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1495996.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKINL"
BASE COUNT          151 a           96 c           89 g          131 t
ORIGIN      
        1 ataaatttat tttgggagtg atagaaaagc ccttcataag ccaatctact tgttcaggtg
       61 tgagagcttt gacatccttt tctgtactgg gccaagtcag tctgccgttc tcaaagcgtt
      121 tatatagtag ccaaaatcct tgaccatccc agtaaagggc tttaaagcgg tctttacgtc
      181 caccacaaaa gagaaaaact tgaccggaga aaggatccaa ttcaaagtgg gttttaacca
      241 gataagccag tgaatcgatt ccttgtctca tatcagtctt cccacacacg agatagacct
      301 gccctaggct agatagatga attgtcatag agcaacacct tatccaaaag ctgttctacc
      361 atttcgagat tgagagattg aaaaaaactt acttcaagct tgccaatacg aatttaaagc
      421 agaatatcat ttctcttttt agaatcaaaa ggacgagatt gtggaat
//
LOCUS       DAVLVF010000209          444 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVLVF010000209 DAVLVF010000000
VERSION     DAVLVF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.209"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..>444)
                     /locus_tag="VYN66_002061"
     CDS             complement(<1..>444)
                     /locus_tag="VYN66_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1495997.1"
                     /translation="KNISLEIDKGIYRVEGENGSGKSTLLQLLAGLETFDSGLKNCVS
                     KDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINTFFDNKIGDNYMTAST
                     GTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISMKES"
BASE COUNT          148 a           83 c           58 g          155 t
ORIGIN      
        1 gctttctttc atggaaatta agcgtttagc aatcatatca atacttcttg catccactgt
       61 tgataaagtc tcatcaataa aaatataatc ccaattttta gcaaaaatta aggataagcc
      121 taatttgaat tttgttccag tagatgctgt catatagtta tctccgattt tattatcaaa
      181 aaaagtatta atttgttctt tatcctcaaa tgatagatgg atttgaaaag aatcacacaa
      241 caactgcaaa ttttcttcta tagtaaatgg aacaacccca agctgattgg tatcaaggta
      301 tagtacatct ttggagacac aattctttaa accactatca aaagtctcta gcccagctaa
      361 aagttgtaga agggtgctct ttcctgaacc attctctcct tcaactctgt aaattccctt
      421 atcaatctct agtgatatat tttt
//
LOCUS       DAVLVF010000210          432 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVLVF010000210 DAVLVF010000000
VERSION     DAVLVF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.210"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            138..>432
                     /locus_tag="VYN66_002062"
     CDS             138..>432
                     /locus_tag="VYN66_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495998.1"
                     /translation="MTRKVPNIEQMSQIECGLCCCLSILHFYKSKETLLDLRRDIEKG
                     RDGYSIGDLKQLLNKRNFDTGSYQVKDVNKISELPLPLITFWDNQHYVVIYKVK"
BASE COUNT          164 a           49 c           79 g          140 t
ORIGIN      
        1 accagaatta agtacggatt tgaatactat gaatacattt tcttttatgc taggagtatc
       61 tggagttgtc tatgaaatat ctcgaaaaca agatgataga ttacttaatg ttttattact
      121 ggagttaaga ggacatgatg actagaaaag tacctaatat tgagcaaatg tctcaaatag
      181 aatgtggttt atgttgctgc ttgtccatac tacatttcta taaaagcaaa gagacattac
      241 tagatttaag acgtgatata gaaaaaggga gagatggtta tagtataggt gacttgaaac
      301 agttattgaa taaacgaaat tttgatactg gctcatatca agttaaagat gtaaataaaa
      361 ttagtgaact tccgttacca ttaataacat tctgggataa tcaacattat gtagttattt
      421 acaaagtaaa aa
//
LOCUS       DAVLVF010000211          425 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVLVF010000211 DAVLVF010000000
VERSION     DAVLVF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.211"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..>425)
                     /locus_tag="VYN66_002063"
     CDS             complement(<1..>425)
                     /locus_tag="VYN66_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1495999.1"
                     /translation="NLLYRKPYIKSISAAITGAILLISSFQMISQNILSQFQDDSDLE
                     LKVAFIVYVGAPILIVLANIISIAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIF
                     LTFVTSLIAFLLNIKIMALIYYSLEDILINDMNLLGLIL"
BASE COUNT          167 a           66 c           53 g          139 t
ORIGIN      
        1 aagtattaac cccaaaagat tcatatcatt tattaaaata tcctctaaag agtagtatat
       61 taaagccata attttaatgt taagtaaaaa tgcaattaat gaagtaacaa atgtcaaaaa
      121 tatagcctca ccaactttaa tcttaaccat ctggtaatta gaagttccta aaatttcaaa
      181 ttgctgaatc tcaatccttt cttgatgcga tgacaaaaat gcaattgaaa taatatttgc
      241 aagtactatc aaaattggtg ctcctacata gacaataaat gccactttta gctctaaatc
      301 actgtcatct tgaaattgag atagtatatt ctgagaaatc atttgaaaac tagaaattag
      361 taatatagct cctgtaattg cagcactgat agattttata taaggcttac gatatagtaa
      421 attcc
//
LOCUS       DAVLVF010000212          422 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVLVF010000212 DAVLVF010000000
VERSION     DAVLVF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.212"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..>422)
                     /locus_tag="VYN66_002064"
     CDS             complement(<1..>422)
                     /locus_tag="VYN66_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1496000.1"
                     /translation="DYIGASIYWAQEAGLNDRIILDHLSISRTIGGHILFPRGGKLET
                     VNQARGGEKGYYDRFDLTLYAIKEWFVENKNTKIGYAIENYHEWFELFSGDDNCKNGF
                     ENFVEFFKLEGFIYEQNKIIDLIKSDLENNQVVFLDKE"
BASE COUNT          137 a           86 c           39 g          160 t
ORIGIN      
        1 cttctttatc taaaaagaca acctgattgt tctctaaatc tgatttaatt aaatctataa
       61 ttttattttg ctcatatata aatccttcta acttgaaaaa ctcgacaaag ttctcaaatc
      121 catttttaca attatcatca ccagaaaata gttcaaacca ttcatggtaa ttctctatag
      181 cataacctat ttttgtattt ttattttcca caaaccattc ttttatagca tatagtgtta
      241 aatcaaaacg atcataatat cctttttctc cacctctagc ctgattaacg gtttctaatt
      301 ttccacctct agggaaaaga atgtgtccac ctattgttct tgaaatactc aaatgatcta
      361 aaattattct gtcgtttaat cctgcctctt gtgcccaata aatagaggct cctatataat
      421 ca
//
LOCUS       DAVLVF010000213          416 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVLVF010000213 DAVLVF010000000
VERSION     DAVLVF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.213"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..>416)
                     /locus_tag="VYN66_002065"
     CDS             complement(<1..>416)
                     /locus_tag="VYN66_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1496001.1"
                     /translation="ISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYAF
                     TKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYDL
                     AKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQF"
BASE COUNT          145 a           76 c           86 g          109 t
ORIGIN      
        1 tcaaactgat tcataatttg aacacgaaaa cgactcatgg cacggctgag atgttggata
       61 atatggaaac gatctagaac gattttagca cacggaaaaa gctgtttagc caagtcatag
      121 taaggactaa acatatccat cgtaatgatt ttcacttgac aacgaacggc tctatcgtag
      181 cgaagaaagt gatttcggat gacagcttgt gttctgcctt caagaacagt gataatatta
      241 agattatcaa aatcttgcgc aatgaaactc atctttccct tagtgaaggc atactcatcc
      301 caagacataa tctttggaag ccgagaaaaa tcatgctcaa agtgaaagtc attgagcttg
      361 cgaatgacag ttgaagttga aatggccagc tgatgggcaa tatcagtcat agaaat
//
LOCUS       DAVLVF010000214          345 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVLVF010000214 DAVLVF010000000
VERSION     DAVLVF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.214"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            231..>345
                     /locus_tag="VYN66_002066"
     CDS             231..>345
                     /locus_tag="VYN66_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1496002.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLG"
BASE COUNT          118 a           53 c           78 g           96 t
ORIGIN      
        1 taccttagtc aagaattaga tgtttcacta tgtttgagta agttgatgat ttcatttgat
       61 aacaggtttg aaactgttag gctaggtggc caaggctaat catagccttg gtttagctga
      121 aaaaacaggt tcaagggttc ctgttgtcaa atgaaatgtg atttaaggta taagaaaaca
      181 cctctgtgct atacttgttg ttcaccacaa acacaaggaa aggcacagag atgcaagaac
      241 attatacccc aaaagggaaa catttgacaa tagataaccg tcgcttgatt gagcggtgga
      301 agaatgaaaa taagtccaat cgtgaaattg caggcttgtt aggaa
//
LOCUS       DAVLVF010000215          331 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVLVF010000215 DAVLVF010000000
VERSION     DAVLVF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.215"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..190)
                     /locus_tag="VYN66_002067"
     CDS             complement(<1..190)
                     /locus_tag="VYN66_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAT RNA binding domain-containing protein"
                     /protein_id="HEV1496003.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKIGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMF"
BASE COUNT          108 a           57 c           39 g          127 t
ORIGIN      
        1 taaacatctc tgtaaaacgg gtcatattat ctctattttt gagaaaaaaa cttttctcaa
       61 tatcaattgg atttacttta tctccaattt ttcttccaaa agctattccc cttccaaaaa
      121 gaagaatatc gatatctttt ttactttgcg caattacggc attgtggttt agtattcgtt
      181 ttatgatcat aaaatttccc ttttaaaata aaaggaacct acttaaaaac atcaagaaac
      241 ttcttccgat gtgattaaat aggttcctgc tcacttgtat tagtgataac atccttttat
      301 ttcaattttt aatggtgaca tgaagcttaa a
//
LOCUS       DAVLVF010000216          327 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVLVF010000216 DAVLVF010000000
VERSION     DAVLVF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.216"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            complement(<1..>327)
                     /locus_tag="VYN66_002068"
     CDS             complement(<1..>327)
                     /locus_tag="VYN66_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1496004.1"
                     /translation="LIQQDSRKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQ
                     IYQLLLFHFQNKDPEKFFGLIEDNLKQVHPIFQTVFKTFLKNKEKIVNALQLPYSNAK
                     LEATNNL"
BASE COUNT           93 a           43 c           77 g          114 t
ORIGIN      
        1 agattattgg tcgcttccaa tttggcgttt gaatagggta gttgaagggc gttgacgatt
       61 ttctctttgt tctttagaaa ggttttaaag acagtctgaa aaataggatg aacctgcttc
      121 agattgtcct caatgagtcc gaaaaatttc tcagggtctt tgttctgaaa gtgaaaaagt
      181 aagagttgat agatctgata gtggtgtttc aagtcttctg aatagcttaa aatcttgtca
      241 agaatttctt tatttgttaa gtgcatgcga aaagtagggc gataaaaacg tttatcgctg
      301 agtttacgac tatcctgttg gatgagt
//
LOCUS       DAVLVF010000217          327 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVLVF010000217 DAVLVF010000000
VERSION     DAVLVF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.217"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            49..327
                     /locus_tag="VYN66_002069"
                     /pseudo
     CDS             49..327
                     /locus_tag="VYN66_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          122 a           54 c           59 g           92 t
ORIGIN      
        1 gataaacaat tctaatatta ggtacatgat taaattgatt gatcgttaat gttggaacag
       61 gcattcaaag agaagcacta cgagaatacg attctccata gtgaccaaga ctggcaatac
      121 caacacgatt cttatcatcg gttcctagag agtaagggaa ttcaagcatc tatgtcacgc
      181 aagggaaaca gccaagacaa cggtaggatg gaatctttct ttggtatttt aaaatccgaa
      241 atgttttatg gctatgagaa aacatttaaa tcacttaatc aattggaaca agccattata
      301 tactatattg attattacaa caacaag
//
LOCUS       DAVLVF010000218          287 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072672
            SAMN33607474-rid17949563.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVLVF010000218 DAVLVF010000000
VERSION     DAVLVF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607474
            Sequence Read Archive: SRR23725599
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721476.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721476.1
            Genome Coverage       :: 273x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:21:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,070
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 1,908
            CDSs (with protein)               :: 1,908
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 56 of 115
            Pseudo Genes (incomplete)         :: 53 of 115
            Pseudo Genes (internal stop)      :: 41 of 115
            Pseudo Genes (multiple problems)  :: 29 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607474-rid17949563.denovo.218"
                     /strain="AUSMDU00072672"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST66"
     gene            <1..>287
                     /locus_tag="VYN66_002070"
     CDS             <1..>287
                     /locus_tag="VYN66_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase 2C family protein"
                     /protein_id="HEV1496005.1"
                     /translation="RLPTFAFIFAKLSEKKLEYMMLGDCVMLVNEMEITDHRVDNLFE
                     KGKNEIKDPIGTNSVLNKKIILQKIRKLSNQPSGYWIGSLDERFLDHAIIN"
BASE COUNT          112 a           32 c           54 g           89 t
ORIGIN      
        1 tcgcttacca acatttgctt ttatatttgc taaactttca gaaaaaaagt tggagtatat
       61 gatgctaggt gactgcgtca tgttagtaaa cgaaatggaa ataacggatc atagagtaga
      121 taacttattt gaaaaaggga aaaatgagat aaaagatcca attggtacaa atagtgtttt
      181 aaataaaaaa attattttac aaaaaatcag aaaattatct aatcaaccga gtgggtactg
      241 gataggttcg ttggatgaga gatttttaga ccatgctatt attaatc
//
